ribose phosphate pyrophosphokinase

Summary

Summary: An enzyme that catalyzes the formation of phosphoribosyl pyrophosphate from ATP and ribose-5-phosphate. EC 2.7.6.1.

Top Publications

  1. ncbi Phosphoribosylpyrophosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene
    B Hove-Jensen
    J Biol Chem 261:6765-71. 1986
  2. ncbi Impaired PRPP-synthesizing capacity compromises cell integrity signalling in Saccharomyces cerevisiae
    Ke Wang
    School of Life Sciences, Heriot Watt University, Edinburgh EH14 4AS, UK
    Microbiology 150:3327-39. 2004
  3. ncbi Novel class III phosphoribosyl diphosphate synthase: structure and properties of the tetrameric, phosphate-activated, non-allosterically inhibited enzyme from Methanocaldococcus jannaschii
    Anders Kadziola
    Centre for Crystallographic Studies, Department of Chemistry, University of Copenhagen, 5 Universitetsparken, DK 2100 Copenhagen Ø, Denmark
    J Mol Biol 354:815-28. 2005
  4. pmc Arts syndrome is caused by loss-of-function mutations in PRPS1
    Arjan P M de Brouwer
    Departments of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Am J Hum Genet 81:507-18. 2007
  5. ncbi Ramifications of impaired PRPP synthesis in Saccharomyces cerevisiae
    S Vavassori
    School of Life Sciences, Heriot Watt University, Edinburgh EH14 4AS, UK
    Biochem Soc Trans 33:1418-20. 2005
  6. pmc The genetic and functional basis of purine nucleotide feedback-resistant phosphoribosylpyrophosphate synthetase superactivity
    M A Becker
    Department of Medicine, University of Chicago, Illinois 60637, USA
    J Clin Invest 96:2133-41. 1995
  7. ncbi In Saccharomyces cerevisiae, impaired PRPP synthesis is accompanied by valproate and Li+ sensitivity
    S Vavassori
    School of Life Sciences, Heriot Watt University, Edinburgh EH14 4AS, UK
    Biochem Soc Trans 33:1154-7. 2005
  8. ncbi Phosphoribosylpyrophosphate synthetase (PRS): a new gene family in Saccharomyces cerevisiae
    A T Carter
    Institute of Food Research, Genetics and Microbiology Department, Colney, Norwich, UK
    Yeast 10:1031-44. 1994
  9. pmc PRS5, the fifth member of the phosphoribosyl pyrophosphate synthetase gene family in Saccharomyces cerevisiae, is essential for cell viability in the absence of either PRS1 or PRS3
    Y Hernando
    Genetics and Microbiology Department, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom
    J Bacteriol 180:6404-7. 1998
  10. pmc Implications of secondary structure prediction and amino acid sequence comparison of class I and class II phosphoribosyl diphosphate synthases on catalysis, regulation, and quaternary structure
    B N Krath
    Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, Copenhagen, Denmark
    Protein Sci 10:2317-24. 2001

Scientific Experts

  • Bjarne Hove-Jensen
  • Arjan Pm de Brouwer
  • Julia W Pridgeon
  • Yukio Kawahara
  • K E Latham
  • Yasukazu Yamada
  • Julio Vera
  • S Ishijima
  • M Camici
  • T D Read
  • W Dudzinska
  • William L Nyhan
  • Anders Kadziola
  • Kaname Kawasugi
  • M A Becker
  • S Vavassori
  • M Schweizer
  • M Tatibana
  • Shanchao Hong
  • T Sonoda
  • T Ishizuka
  • B N Krath
  • Sabela Balboa
  • Mark J Koenigsknecht
  • L M Schweizer
  • Xuezhong Liu
  • Fujio Takeuchi
  • Rena Shimizu
  • K Kita
  • Takayuki Morisaki
  • Alberto Jimenez
  • Taizo Iizasa
  • Toru Nakamura
  • Sudha Mannava
  • Maikun Teng
  • Silke Koslowsky
  • Xu Li
  • Md Atiar Rahman
  • Sumitaka Hase
  • Hee Jin Kim
  • Sheng Li
  • Megumi Shimaoka
  • Wenying Tang
  • Song Mee Bae
  • Hairong Huang
  • Atsushi Kaida
  • K Wang
  • Atsuo Taniguchi
  • H B Napolitano
  • Matthew M Hewitt
  • Cydney B Nielsen
  • Ke Wang
  • Pablo Garcia-Pavia
  • Shin Fujimori
  • Kimiyoshi Ichida
  • M Garcia-Gil
  • Y Hernando
  • I Ahmad
  • Tine A Eriksen
  • Orsolya Mezei
  • M Ahmed
  • Chen Chen
  • Jing Yv
  • P R Smith
  • Weijie Wang
  • Xinyou Yv
  • Yuan Lv
  • Dan Zhou
  • Lei Ma
  • Yvting Ye
  • Changliang Zhu
  • Yan Sun
  • Xuelian Chang
  • Bo Shen
  • Linna Shi
  • Donghui Zhang
  • Ping Zou
  • A W Karzai
  • A K Bentsen
  • K W Harlow
  • T Kawaguchi
  • H Nakano
  • Jose L Lopez
  • Cristina Gianzo
  • Jesus L Romalde
  • José Bermúdez-Crespo
  • T Yamaoka
  • Xiaomei Ouyang
  • Li L Du
  • Heng Xu

Detail Information

Publications107 found, 100 shown here

  1. ncbi Phosphoribosylpyrophosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene
    B Hove-Jensen
    J Biol Chem 261:6765-71. 1986
    ..Transcription terminated approximately 35 nucleotides downstream from the UAA translation stop codon, within a Thy-rich region following an inverted repeat sequence, indicative of an rho-independent transcription terminator...
  2. ncbi Impaired PRPP-synthesizing capacity compromises cell integrity signalling in Saccharomyces cerevisiae
    Ke Wang
    School of Life Sciences, Heriot Watt University, Edinburgh EH14 4AS, UK
    Microbiology 150:3327-39. 2004
    ..It is postulated that Prs polypeptides play a significant role in the remodelling of the cell wall and may have a direct involvement in cell integrity signalling...
  3. ncbi Novel class III phosphoribosyl diphosphate synthase: structure and properties of the tetrameric, phosphate-activated, non-allosterically inhibited enzyme from Methanocaldococcus jannaschii
    Anders Kadziola
    Centre for Crystallographic Studies, Department of Chemistry, University of Copenhagen, 5 Universitetsparken, DK 2100 Copenhagen Ø, Denmark
    J Mol Biol 354:815-28. 2005
    ....
  4. pmc Arts syndrome is caused by loss-of-function mutations in PRPS1
    Arjan P M de Brouwer
    Departments of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Am J Hum Genet 81:507-18. 2007
    ..To replenish low levels of purines, treatment with S-adenosylmethionine theoretically could have therapeutic efficacy, and a clinical trial involving the two affected Australian brothers is currently underway...
  5. ncbi Ramifications of impaired PRPP synthesis in Saccharomyces cerevisiae
    S Vavassori
    School of Life Sciences, Heriot Watt University, Edinburgh EH14 4AS, UK
    Biochem Soc Trans 33:1418-20. 2005
    ..This approach provides insight into the co-ordination of different biological processes...
  6. pmc The genetic and functional basis of purine nucleotide feedback-resistant phosphoribosylpyrophosphate synthetase superactivity
    M A Becker
    Department of Medicine, University of Chicago, Illinois 60637, USA
    J Clin Invest 96:2133-41. 1995
    ..The functional consequences of these mutations provide a tenable basis for the enhanced production of phosphoribosylpyrophosphate, purine nucleotides, and uric acid that are the biochemical hallmarks of PRS superactivity...
  7. ncbi In Saccharomyces cerevisiae, impaired PRPP synthesis is accompanied by valproate and Li+ sensitivity
    S Vavassori
    School of Life Sciences, Heriot Watt University, Edinburgh EH14 4AS, UK
    Biochem Soc Trans 33:1154-7. 2005
    ..Interference with the complement of PRS genes in S. cerevisiae has far-reaching consequences for yeast physiology and has uncovered unexpected metabolic links including cell wall integrity and phospholipid metabolism...
  8. ncbi Phosphoribosylpyrophosphate synthetase (PRS): a new gene family in Saccharomyces cerevisiae
    A T Carter
    Institute of Food Research, Genetics and Microbiology Department, Colney, Norwich, UK
    Yeast 10:1031-44. 1994
    ..The results presented here suggest that the presence of this NHR is not detrimental to the function of the gene. To date the possibility of protein splicing can be neither proven nor disputed...
  9. pmc PRS5, the fifth member of the phosphoribosyl pyrophosphate synthetase gene family in Saccharomyces cerevisiae, is essential for cell viability in the absence of either PRS1 or PRS3
    Y Hernando
    Genetics and Microbiology Department, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom
    J Bacteriol 180:6404-7. 1998
    ..However, simultaneous deletion of PRS1 and PRS5 or PRS3 and PRS5 rendered the strains inviable, which implies that PRS5 plays an important role in the maintenance of PRS function in S. cerevisiae...
  10. pmc Implications of secondary structure prediction and amino acid sequence comparison of class I and class II phosphoribosyl diphosphate synthases on catalysis, regulation, and quaternary structure
    B N Krath
    Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, Copenhagen, Denmark
    Protein Sci 10:2317-24. 2001
    ..subtilis enzyme show little conservation in the spinach enzyme. In contrast, residues of the active site of B. subtilis PRPP synthase show extensive conservation in spinach PRPP synthase isozyme 4...
  11. ncbi Heterooligomeric phosphoribosyl diphosphate synthase of Saccharomyces cerevisiae: combinatorial expression of the five PRS genes in Escherichia coli
    Bjarne Hove-Jensen
    Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, DK 1307 Copenhagen, Denmark
    J Biol Chem 279:40345-50. 2004
    ..The activity of PRPP synthases containing subunits 1 and 3 or subunits 1, 2, and 5 was found to be dependent on Pi, to be temperature-sensitive, and inhibited by ADP...
  12. pmc Mutations in PRPS1, which encodes the phosphoribosyl pyrophosphate synthetase enzyme critical for nucleotide biosynthesis, cause hereditary peripheral neuropathy with hearing loss and optic neuropathy (cmtx5)
    Hee Jin Kim
    Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Gangnam Gu, Seoul, Korea
    Am J Hum Genet 81:552-8. 2007
    ....
  13. pmc Crystal structure of human phosphoribosylpyrophosphate synthetase 1 reveals a novel allosteric site
    Sheng Li
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
    Biochem J 401:39-47. 2007
    ....
  14. ncbi Structural basis for the function of Bacillus subtilis phosphoribosyl-pyrophosphate synthetase
    T A Eriksen
    Center for Crystallographic Studies, Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK 2100 Copenhagen, Denmark
    Nat Struct Biol 7:303-8. 2000
    ..In addition to identifying residues important for binding substrates and effectors, the structures suggest a novel mode of allosteric regulation...
  15. pmc Higher biomass accumulation by increasing phosphoribosylpyrophosphate synthetase activity in Arabidopsis thaliana and Nicotiana tabacum
    Silke Koslowsky
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476 Potsdam Golm, Germany
    Plant Biotechnol J 6:281-94. 2008
    ..This study provides evidence that the supply of PRPP co-limits growth rates, and has obvious implications for biotechnological strategies aiming to increase plant biomass as an alternative renewable energy source...
  16. ncbi Identification and active expression of the Mycobacterium tuberculosis gene encoding 5-phospho-{alpha}-d-ribose-1-diphosphate: decaprenyl-phosphate 5-phosphoribosyltransferase, the first enzyme committed to decaprenylphosphoryl-d-arabinose synthesis
    Hairong Huang
    Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523 1682, USA
    J Biol Chem 280:24539-43. 2005
    ..tuberculosis, and the tuberculosis drug ethambutol inhibits other steps in arabinan biosynthesis. Thus the Rv3806c-encoded enzyme appears to be a good target for the development of new tuberculosis drugs...
  17. ncbi Disorders associated with purine and pyrimidine metabolism
    N L Edwards
    Spec Top Endocrinol Metab 6:95-140. 1984
    ..These will no doubt expand our understanding of the pathogenesis of these disorders and provide innovative therapeutic approaches...
  18. ncbi Phosphoribosylpyrophosphate synthetase overactivity as a cause of uric acid overproduction in a young woman
    Pablo Garcia-Pavia
    La Paz University Hospital, Madrid, Spain
    Arthritis Rheum 48:2036-41. 2003
    ..This is the first reported instance of PRS overactivity in which the propositus and sole affected family member is a woman...
  19. ncbi The purine metabolism of human erythrocytes
    W Dudzinska
    Department of Biochemistry, Faculty of Natural Sciences, University of Szczecin, Szczecin, Poland
    Biochemistry (Mosc) 71:467-75. 2006
    ..We also mention purine metabolism disorders and their clinical consequences. The literature is fragmentary because studies have concentrated only on selected aspects of purine metabolism; hence the need for a synthetic approach...
  20. ncbi [Phosphoribosylpyrophosphate(PRPP) synthetase]
    Kaname Kawasugi
    Department of Allergy and Rheumatology, University of Tokyo Graduate School of Medicine
    Nihon Rinsho 61:20-3. 2003
  21. ncbi [Urate production and regulation]
    Takayuki Morisaki
    Department of Bioscience, National Cardiovascular Center Research Institute
    Nihon Rinsho 66:653-8. 2008
    ..Here, regulation of purine biosynthesis pathway and relevant mechanisms are discussed. Genetic mechanisms for urate overproduction, dietary effects on urate amount and new drug therapy inhibiting urate production are also discussed...
  22. ncbi [Historical review of gout and hyperuricemia investigations]
    Toru Nakamura
    Department of Internal Medicine, Fukui University
    Nihon Rinsho 66:624-35. 2008
    ..However, the mechanism in majority of the overproduction has not yet been clarified and is currently under investigation...
  23. ncbi Overexpression of Bacillus subtilis phosphoribosylpyrophosphate synthetase and crystallization and preliminary X-ray characterization of the free enzyme and its substrate-effector complexes
    A K Bentsen
    Center for Enzyme Research, Department of Protein Chemistry, University of Copenhagen, Denmark
    Proteins 24:238-46. 1996
    ..The availability of the different complexes should allow questions regarding the molecular mechanisms of catalysis and allostery in PRPP synthetase to be addressed...
  24. ncbi [Genetic testing and gene-based testing for hyperuricemia and gout]
    Atsuo Taniguchi
    Institute of Rheumatology, Tokyo Women s Medical University
    Nihon Rinsho 63:285-9. 2005
  25. ncbi Phosphoribosylpyrophosphate synthetase and the regulation of phosphoribosylpyrophosphate production in human cells
    M A Becker
    The Unversity of Chicago, University of Chicago Medical Center, Illinois 60637, USA
    Prog Nucleic Acid Res Mol Biol 69:115-48. 2001
    ....
  26. ncbi [Hyperuricemia associated with inborn errors of purine metabolism: screening, enzymatic and genetic diagnosis]
    Yasukazu Yamada
    Department of Genetics, Institute for Developmental Research, Aichi Human Service Center
    Nihon Rinsho 61:278-83. 2003
  27. ncbi Rat liver phosphoribosylpyrophosphate synthetase is activated by free Mg2+ in a manner that overcomes its inhibition by nucleotides
    T Sonoda
    Department of Biochemistry, Chiba University School of Medicine, Inohana 1 8 1, Chuo Ku, Chiba 260 8670, Japan
    Biochim Biophys Acta 1387:32-40. 1998
    ..6) We propose that MgGDP binds to a regulatory site of PRS I and PRS II and MgADP to the substrate MgATP site and also the regulatory site. The allosteric interaction of the regulatory site and the Mg2+ binding site is also considered...
  28. ncbi Inactivation of Escherichia coli phosphoribosylpyrophosphate synthetase by the 2',3'-dialdehyde derivative of ATP. Identification of active site lysines
    I Hilden
    Center for Enzyme Research, University of Copenhagen, Denmark
    J Biol Chem 270:20730-6. 1995
    ..These results imply a functional role for at least two of the identified amino acid residues...
  29. ncbi Partial reconstitution of mammalian phosphoribosylpyrophosphate synthetase in Escherichia coli cells. Coexpression of catalytic subunits with the 39-kDa associated protein leads to formation of soluble multimeric complexes of various compositions
    S Ishijima
    Department of Biochemistry, Chiba University School of Medicine, Japan
    Biochim Biophys Acta 1342:28-36. 1997
    ....
  30. ncbi [Primary underproductive hypouricemia]
    Kimiyoshi Ichida
    Division of Kidney and Hypertension, Jikei University School of Medicine
    Nihon Rinsho 61:364-7. 2003
  31. ncbi Kinetic and regulatory properties of rat liver phosphoribosylpyrophosphate synthetase complex are partly distinct from those of isolated recombinant component catalytic subunits
    T Sonoda
    Department of Biochemistry, Chiba University School of Medicine
    J Biochem 122:635-40. 1997
    ....
  32. pmc Binding of cations in Bacillus subtilis phosphoribosyldiphosphate synthetase and their role in catalysis
    Tine A Eriksen
    Centre for Crystallographic Studies, Department of Chemistry, University of Copenhagen, 2100 Copenhagen, Denmark
    Protein Sci 11:271-9. 2002
    ..This strongly indicates that the binding of both substrates must lead to a stabilized conformation of the loop region, which remains unresolved in the known PRPPsase complex structures...
  33. ncbi Catalytic residues Lys197 and Arg199 of Bacillus subtilis phosphoribosyl diphosphate synthase. Alanine-scanning mutagenesis of the flexible catalytic loop
    Bjarne Hove-Jensen
    Department of Biological Chemistry, Institute of Molecular Biology and Physiology, University of Copenhagen, Denmark
    FEBS J 272:3631-9. 2005
    ..The data presented show that Lys197 and Arg199 are important in stabilization of the transition state...
  34. pmc Regulation of Escherichia coli glnB, prsA, and speA by the purine repressor
    B He
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907 1153
    J Bacteriol 175:3598-606. 1993
    ..The results indicated two- to threefold repression of these genes by purR. Coregulation by purR provides evidence that expands the pur regulon to include glnB, prsA, and speA. These genes have functions related to nucleotide metabolism...
  35. pmc Suppression of thermosensitive initiation of DNA replication in a dnaR mutant of Escherichia coli by a rifampin resistance mutation in the rpoB gene
    Y Sakakibara
    Department of Biochemistry and Cellular Biology, National Institute of Health, Tokyo, Japan
    J Bacteriol 177:733-7. 1995
    ..I propose that the dnaR product has a key role in transcriptional activation of the replication origin for the dnaA-dependent initiation of DNA replication...
  36. pmc Chromosomal location of the gene encoding phosphoribosylpyrophosphate synthetase in Escherichia coli
    B Hove-Jensen
    J Bacteriol 154:177-84. 1983
    ..The contribution of each mutation to the phenotype of the mutant has been evaluated...
  37. ncbi Nucleotide and deduced amino acid sequences of two distinct cDNAs for rat phosphoribosylpyrophosphate synthetase
    M Taira
    Department of Biochemistry, Chiba University, School of Medicine, Japan
    J Biol Chem 262:14867-70. 1987
    ..Transcripts of 2.3 and 3.7 kilobases were detected in YS cells and rat liver by Northern blot analysis, using PRS I and II cDNAs respectively, as probes. In the liver, the expression of both genes increased after partial hepatectomy...
  38. pmc Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae
    T D Read
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 31:2134-47. 2003
    ..pneumoniae. This crucial addition to the set of completed Chlamydiaceae genome sequences is enabling dissection of the roles played by niche-specific genes in these important bacterial pathogens...
  39. ncbi Characterization of novel genes expressed specifically in the sexual organs of the planarian Dugesia ryukyuensis
    Sumitaka Hase
    Department of Biological Sciences and Informatics, Keio University, Kouhoku, Yokohama, Japan
    Int J Dev Biol 51:345-9. 2007
    ..These genes are useful markers for elucidating the sexualization process...
  40. pmc Preliminary crystallographic analysis of sugar cane phosphoribosylpyrophosphate synthase
    H B Napolitano
    Instituto de Fisica de Sao Carlos, Universidade de Sao Paulo, Caixa Postal 369, 13560 970 Sao Carlos, SP, Brazil
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:49-51. 2005
    ..2, b = 152.6, c = 149.3 A. The crystals diffract to a maximum resolution of 3.3 A and a complete data set to 3.5 A resolution was collected and analysed...
  41. ncbi Primary structure of the tms and prs genes of Bacillus subtilis
    D Nilsson
    Enzyme Division, University Institute of Biological Chemistry B, Copenhagen, Denmark
    Mol Gen Genet 218:565-71. 1989
    ..The deduced Tms amino acid sequence was found to be 43% similar to the deduced amino acid sequence of ecourfl, a gene of E. coli with unknown function...
  42. pmc Direct role of nucleotide metabolism in C-MYC-dependent proliferation of melanoma cells
    Sudha Mannava
    Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA
    Cell Cycle 7:2392-400. 2008
    ..Our data establish a novel functional link between C-MYC and dNTP metabolism and identify its role in proliferation of tumor cells...
  43. pmc Tissue specificity and evolution of meristematic WOX3 function
    Rena Shimizu
    Department of Plant Biology, Cornell University, Ithaca, New York 14853, USA
    Plant Physiol 149:841-50. 2009
    ....
  44. pmc Loss-of-function mutations in the PRPS1 gene cause a type of nonsyndromic X-linked sensorineural deafness, DFN2
    Xuezhong Liu
    Department of Otolaryngology, University of Miami, FL 33136, USA
    Am J Hum Genet 86:65-71. 2010
    ..Being the second identified gene associated with X-linked nonsyndromic deafness, PRPS1 will be a good candidate gene for genetic testing for X-linked nonsyndromic hearing loss...
  45. ncbi Effects of X chromosome number and parental origin on X-linked gene expression in preimplantation mouse embryos
    K E Latham
    The Fels Institute for Cancer Research and Molecular Biology and Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    Biol Reprod 63:64-73. 2000
    ..The results also indicate that early dosage compensation in preimplantation embryos may normally be reversible, thus providing flexibility to meet different developmental requirements of the embryonic and extraembryonic lineages...
  46. ncbi A human testis-specific mRNA for phosphoribosylpyrophosphate synthetase that initiates from a non-AUG codon
    M Taira
    Department of Biochemistry, Chiba University School of Medicine, Japan
    J Biol Chem 265:16491-7. 1990
    ..These results suggested that the synthesis of the nascent polypeptide could initiate with methionine at the position corresponding to the ACG codon...
  47. ncbi Cloning of cDNAs for human phosphoribosylpyrophosphate synthetases 1 and 2 and X chromosome localization of PRPS1 and PRPS2 genes
    M A Becker
    Department of Medicine, University of Chicago, Illinois 60637
    Genomics 8:555-61. 1990
    ..Human HPRT and PRPS1 loci are not closely linked. Despite marked cDNA and deduced amino acid sequence homology, human PRS 1 and PRS 2 isoforms are encoded by genes widely separated on the X chromosome...
  48. ncbi Expression of rat phosphoribosylpyrophosphate synthetase subunits I and II in Escherichia coli. Isolation and characterization of the recombinant isoforms
    S Ishijima
    Department of Biochemistry, Chiba University School of Medicine, Japan
    J Biol Chem 266:15693-7. 1991
    ..Inhibition of rPRS I and rPRS II by 0.3 mM ADP was 87 and 54%, respectively, and inhibition by 1 mM GDP was 93 and 24%, respectively. rPRS II was 180-fold more sensitive than rPRS I to heat inactivation at 49 degrees C...
  49. pmc Complete cDNA sequence of rat phosphoribosylpyrophosphate synthetase subunit I (PRS I)
    S Ishijima
    Department of Biochemistry, Chiba University School of Medicine, Japan
    Nucleic Acids Res 17:8860. 1989
  50. ncbi Regulation of purine nucleotide synthesis in human B lymphoblasts with both hypoxanthine-guanine phosphoribosyltransferase deficiency and phosphoribosylpyrophosphate synthetase superactivity
    M A Becker
    Department of Medicine, University of Chicago Pritzker School of Medicine, Illinois 60637
    J Biol Chem 267:4317-21. 1992
    ....
  51. ncbi Accelerated transcription of PRPS1 in X-linked overactivity of normal human phosphoribosylpyrophosphate synthetase
    M Ahmed
    Rheumatology Section, Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 274:7482-8. 1999
    ..The genetic basis of disordered PRPS1 transcription remains unresolved; normal- and patient-derived PRPS1s share nucleotide sequence identity at least 850 base pairs 5' to the consensus transcription initiation site...
  52. pmc Ribose-phosphate pyrophosphokinase 1 (PRPS1) associated with deltamethrin resistance in Culex pipiens pallens
    Shanchao Hong
    Department of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu 210029, People s Republic of China
    Parasitol Res 112:847-54. 2013
    ..Cells transfected with PRPS1 had an increased resistance to deltamethrin compared with control cells. These results suggested that the increased expression level of PRPS1 may play roles in the regulation of deltamethrin resistance...
  53. ncbi Molecular cloning and sequencing of human cDNA for phosphoribosyl pyrophosphate synthetase subunit II
    T Iizasa
    Department of Biochemistry, Chiba University School of Medicine, Japan
    FEBS Lett 244:47-50. 1989
    ..This amino acid homology seems to be the highest so far reported for enzymes involved in nucleotide metabolism and glycolysis. The highly conserved structure may be required for unique catalysis and rigid regulation of this enzyme...
  54. ncbi Tissue-differential expression of two distinct genes for phosphoribosyl pyrophosphate synthetase and existence of the testis-specific transcript
    M Taira
    Department of Biochemistry, Chiba University School of Medicine, Japan
    Biochim Biophys Acta 1007:203-8. 1989
    ..In the testis, an additional PRPS1-related transcript of 1.4 kb was noted in rats, mice and humans. This transcript may belong to a group of testis-specific gene expressions or functions...
  55. ncbi The effect of protein expression of Streptococcus pneumoniae by blood
    Song Mee Bae
    Division of Bacterial Respiratory Infections, Center for Infectious Disease, National Institute of Health, Korea Center for Diseases control and Prevention, 5 Nokbun dong, Eunpyung Ku, Seoul, 122 701, Korea
    J Biochem Mol Biol 39:703-8. 2006
    ..This global approach can provide a better understanding of S. pneumoniae adaptation to its human host and a clue for its pathogenicity...
  56. pmc Expression, purification, crystallization and preliminary X-ray diffraction analysis of human phosphoribosyl pyrophosphate synthetase 1 (PRS1)
    Wenying Tang
    Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230027, People s Republic of China
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:432-4. 2006
    ..846, c = 61.857 angstroms, assuming two molecules in the asymmetric unit and a volume-to-weight ratio of 2.4 angstroms3 Da(-1), which was consistent with the result calculated from the self-rotation function...
  57. ncbi Rat liver phosphoribosyl pyrophosphate synthetase: existence of the purified enzyme as heterogeneous aggregates and identification of the catalytic subunit
    K Kita
    Department of Biochemistry, Chiba University School of Medicine
    J Biochem 105:736-41. 1989
    ..5) The amino acid composition of the 34-kDa subunit and the amino acid sequences of its N-terminal region and of two tryptic peptides were determined. The results are in accord with the results of cDNA analyses...
  58. pmc Complete cDNA sequence of rat phosphoribosylpyrophosphate synthetase subunit II (PRS II)
    S Ishijima
    Department of Biochemistry, Chiba University School of Medicine, Japan
    Nucleic Acids Res 17:8859. 1989
  59. pmc Structure of the gene encoding phosphoribosylpyrophosphate synthetase (prsA) in Salmonella typhimurium
    S G Bower
    Department of Biochemistry, University of Illinois, Urbana 61801
    J Bacteriol 170:3243-8. 1988
    ..The S. typhimurium leader contains a 115-base-pair insert relative to the E. coli leader. The insert appears to have no functional significance...
  60. ncbi Cloning and characterization of the prs gene encoding phosphoribosylpyrophosphate synthetase of Escherichia coli
    B Hove-Jensen
    Mol Gen Genet 201:269-76. 1985
    ..By deletion analysis and by cloning fragments of this leader sequence in a galK expression vector it was found to contain the prs promoter as well as a potential transcription termination site...
  61. ncbi Phosphoribosylpyrophosphate synthetase of Escherichia coli, Identification of a mutant enzyme
    B Hove-Jensen
    Eur J Biochem 126:327-32. 1982
    ..Our data suggest that the mutant harbors a mutation in the structural gene for PRib-PP synthetase. The mutation responsible for the altered PRib-PP synthetase was located in the purB-hemA region at 26 min on the recalibrated linkage map...
  62. ncbi Effect of amplification of desensitized purF and prs on inosine accumulation in Escherichia coli
    Megumi Shimaoka
    Fermentation and Biotechnology Laboratories, Ajinomoto Co, Inc, 1 1 Suzuki cho, Kawasaki ku, Kawasaki shi, Kanagawa 210 8681, Japan
    J Biosci Bioeng 103:255-61. 2007
    ..The spontaneous mutant I-9m harboring only pMWKQ and I-9m harboring both pMWKQ and pSTVDA (a plasmid having the desensitized-prs) accumulated 6.7 g/l and 7.5 g/l inosine, respectively, from 40 g/l glucose in a 3-d culture...
  63. ncbi Pyrimidine nucleotides impair phosphoribosylpyrophosphate (PRPP) synthetase subunit aggregation by sequestering magnesium. A mechanism for the decreased PRPP synthetase activity in hereditary erythrocyte pyrimidine 5'-nucleotidase deficiency
    N A Lachant
    Department of Medicine, Harbor UCLA Medical Center, UCLA School of Medicine, Torrance 90502
    Biochim Biophys Acta 994:81-8. 1989
    ....
  64. doi Proteomics and multilocus sequence analysis confirm intraspecific variability of Vibrio tapetis
    Sabela Balboa
    Departamento de Microbiologia y Parasitologia, CIBUS, Universidad de Santiago de Compostela, Santiago de Compostela, Spain
    FEMS Microbiol Lett 324:80-7. 2011
    ....
  65. pmc Thiamine biosynthesis can be used to dissect metabolic integration
    Mark J Koenigsknecht
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    Trends Microbiol 18:240-7. 2010
    ....
  66. pmc PRPS1 mutations: four distinct syndromes and potential treatment
    Arjan P M de Brouwer
    Department of Human Genetics, Nijmegen Centre for Molecular Life Sciences and Institute of Genetic and Metabolic Diseases, Radboud University Nijmegen Medical Centre, 6500 HB Nijmegen, The Netherlands
    Am J Hum Genet 86:506-18. 2010
    ..C., unpublished data)...
  67. ncbi Proteome analysis for 3T3-L1 adipocyte differentiation
    Md Atiar Rahman
    Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk 712 714, Korea
    J Microbiol Biotechnol 18:1895-902. 2008
    ..In conclusion, our results provide valuable information for further understanding of the adipogenic process...
  68. pmc Phosphoribosyl pyrophosphate synthetase activity affects growth and riboflavin production in Ashbya gossypii
    Alberto Jimenez
    Instituto de Microbiología Bioquímica and Departamento de Microbiología y Genética, CSIC Universidad de Salamanca, Campus Miguel de Unamuno, 37007, Salamanca, Spain
    BMC Biotechnol 8:67. 2008
    ..The industrial production of this vitamin using the fungus Ashbya gossypii is an important biotechnological process that is strongly influenced by substrate availability...
  69. ncbi [Increased activity of PRPP synthetase]
    Taizo Iizasa
    Department of Internal Medicine, Itoh Municipal Hospital
    Nihon Rinsho 66:694-8. 2008
    ..The mechanism producing increased mRNA is, however, not elucidated. The Japanese case has been found to be caused by the second mechanism...
  70. pmc Ribose metabolism and nucleic acid synthesis in normal and glucose-6-phosphate dehydrogenase-deficient human erythrocytes infected with Plasmodium falciparum
    E F Roth
    J Clin Invest 77:1129-35. 1986
    ....
  71. ncbi 5'-aminoimidazole-4-carboxamide riboside induces apoptosis in human neuroblastoma cells
    M Garcia-Gil
    Dipartimento di Fisiologia e Biochimica, Universita di Pisa, Via S Maria, 55 56100, Pisa, Italy
    Neuroscience 117:811-20. 2003
    ..Our results raise the intriguing clue that the neurotoxic effect of AICA riboside on the developing brain might contribute to the neurological manifestations of syndromes related to purine dismetabolisms...
  72. ncbi Regulation of purine synthesis de novo in human fibroblasts by purine nucleotides and phosphoribosylpyrophosphate
    M A Becker
    Department of Medicine, University of Chicago Pritzker School of Medicine, Illinois 60637
    J Biol Chem 262:14531-7. 1987
    ..3) Activation of amido PRT by PP-Rib-P is nearly maximal at base line in fibroblasts with regulatory defects in PP-Rib-P synthetase...
  73. ncbi Detection of potential enzyme targets by metabolic modelling and optimization: application to a simple enzymopathy
    Julio Vera
    Systems Biology and Bioinformatics Group, University of Rostock, Albert Einstein Street 21, 18051, Germany
    Bioinformatics 23:2281-9. 2007
    ..Each solution consists of a set of predicted values for metabolites, initial substrates and enzyme activities, which describe a biologically acceptable steady state of the system that shifts the pathologic state towards a healthy state...
  74. ncbi Effects of phosphate limitation on expression of genes involved in pyrimidine synthesis and salvaging in Arabidopsis
    Matthew M Hewitt
    Department of Biological Sciences, Goucher College, Baltimore, MD 21204 2794, USA
    Plant Physiol Biochem 43:91-9. 2005
    ..PRS2 mRNA levels increased twofold in roots and shoots of P-starved plants, while PRS3 was constitutively-expressed. PRS3 may play a novel role in providing PRPP to cellular metabolism under low P availability...
  75. ncbi Inherited hyperuricemic disorders
    William L Nyhan
    Departmen tof Pediatrics, University of California, San Diego, La Jolla, CA 92093, USA
    Contrib Nephrol 147:22-34. 2005
    ..The undersecretion disorders are autosomal dominantly inherited and are heterogeneous. A major number of these patients result from mutations in the gene that codes for uromodulin. Treatment is with allopurinol...
  76. pmc Patterns of intron gain and loss in fungi
    Cydney B Nielsen
    Department of Biology, Massachusetts Institute of Technology Cambridge, Massachusetts, USA
    PLoS Biol 2:e422. 2004
    ..Contrary to standard models, we find no increased frequency of intron loss toward the 3' ends of genes. Thus, recent intron dynamics do not support a model whereby 5' intron positional bias is generated solely by 3'-biased intron loss...
  77. ncbi A novel 39-kDa phosphoribosylpyrophosphate synthetase-associated protein of rat liver. Cloning, high sequence similarity to the catalytic subunits, and a negative regulatory role
    K Kita
    Department of Biochemistry, Chiba University School of Medicine, Japan
    J Biol Chem 269:8334-40. 1994
    ..Based on these results, we propose that PAP39, the major component of PAPs, plays a negative regulatory role in PRPP synthesis and hence is an important factor controlling nucleotide syntheses in general...
  78. ncbi Hepatic cytochrome p450-2A and phosphoribosylpyrophosphate synthetase-associated protein mRNA are induced in gerbils after consumption of isoflavone-containing protein
    Orsolya Mezei
    Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
    J Nutr 132:2538-44. 2002
    ..05) in gerbils fed all diets containing isoflavones. Induction of these two mRNA by soy may be due to the aryl hydrocarbon receptor element in the promoter region of both genes...
  79. ncbi The human phosphoribosylpyrophosphate synthetase-associated protein 39 gene (PRPSAP1) is located in the chromosome region 17q24-q25
    T Ishizuka
    Department of Biochemistry, Chiba University, School of Medicine, Japan
    Genomics 33:332-4. 1996
  80. ncbi Overexpression of the normal phosphoribosylpyrophosphate synthetase 1 isoform underlies catalytic superactivity of human phosphoribosylpyrophosphate synthetase
    M A Becker
    Rheumatology Section, Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 271:19894-9. 1996
    ..In lymphoblasts, however, expression of this alteration is attenuated, explaining the absence of phenotypic expression of PRS catalytic superactivity in these cells...
  81. ncbi Temperature-sensitive mutations in various genes of Escherichia coli K12 can be suppressed by the ssrA gene for 10Sa RNA (tmRNA)
    H Nakano
    Department of Biophysics, Graduate School of Science, Kyoto University, Japan
    Mol Genet Genomics 265:615-21. 2001
    ..Considering the fact that ssrA-suppressible mutants could be isolated as temperature-sensitive mutants with mutations in different genes, it seems evident that trans-translation can occur on mRNA that is not lacking its stop codon...
  82. ncbi 10Sa RNA complements the temperature-sensitive phenotype caused by a mutation in the phosphoribosyl pyrophosphate synthetase (prs) gene in Escherichia coli
    H Ando
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    Genes Genet Syst 71:47-50. 1996
    ..Sequencing of the mutant prs gene of TS101 showed that residues 215, cysteine, in the encoded protein had been changed to tyrosine. That such a mutant exists suggests that 10Sa RNA associate with the prs gene product in a functional way...
  83. pmc Protein factors associated with the SsrA.SmpB tagging and ribosome rescue complex
    A W Karzai
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 98:3040-4. 2001
    ....
  84. ncbi The importance of the five phosphoribosyl-pyrophosphate synthetase (Prs) gene products of Saccharomyces cerevisiae in the maintenance of cell integrity and the subcellular localization of Prs1p
    R Schneiter
    Department of Biological Sciences, Heriot Watt University, Edinburgh EH14 4AS, UK
    Microbiology 146:3269-78. 2000
    ..The fact that Deltaprs1 and Deltaprs3 are hypersensitive to caffeine and unable to recover from exposure to it as judged by the release of alkaline phosphatase points to a possible link between Prs and the maintenance of cell integrity...
  85. ncbi The defective phosphoribosyl diphosphate synthase in a temperature-sensitive prs-2 mutant of Escherichia coli is compensated by increased enzyme synthesis
    D A Post
    Department of Biochemistry, University of Illinois, Urbana 61801, USA
    Microbiology 142:359-65. 1996
    ..In cells harbouring both mutations, the C --> T mutation appeared to compensate for the G --> A mutation by increasing the amount of a partially defective enzyme at the permissive temperature...
  86. ncbi [Metabolism of purine nucleotides and the production of uric acid]
    T Yamaoka
    Otsuka Department of Clinical and Molecular Nutrition, School of Medicine, University of Tokushima
    Nihon Rinsho 54:3188-94. 1996
    ....
  87. ncbi The yeast PRS3 gene is required for cell integrity, cell cycle arrest upon nutrient deprivation, ion homeostasis and the proper organization of the actin cytoskeleton
    K M Binley
    Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Yeast 15:1459-69. 1999
    ..In the same colony-sectoring assay, an mpk1 allele was also recovered. Multicopy PRS3 rescued the caffeine sensitivity of this mpk1 allele...
  88. ncbi Purine biosynthesis de novo in bovine retina: purification and characterization of amidophosphoribosyl transferase and phosphoribosyl pyrophosphate synthetase
    I A Kian
    Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St Petersburg, 194223, Russia
    Biochemistry (Mosc) 64:648-51. 1999
    ..The sensitivity of the retinal PRPP synthetase to inhibition by ADP and AMP was significantly lower than that of the enzyme from the liver...
  89. ncbi [Idiopathic hyperuricemia]
    Shin Fujimori
    Department of Internal Medicine, Teikyo University School of Medicine
    Nihon Rinsho 61:172-7. 2003
  90. ncbi Linkage of phosphoribosylpyrophosphate synthetases 1 and 2, Prps1 and Prps2, on the mouse X chromosome
    K Lee
    Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263
    Mamm Genome 5:612-5. 1994
    ....
  91. ncbi Molecular cloning of a human cDNA for the 41-kDa phosphoribosylpyrophosphate synthetase-associated protein
    R Katashima
    Otsuka Department of Clinical and Molecular Nutrition, School of Medicine, University of Tokushima, Japan
    Biochim Biophys Acta 1396:245-50. 1998
    ..Southern blot analysis suggested that the PAP41 gene exists as a single copy in the human genome. The single PAP41 mRNA of about 2.1 kb was shown to be present in five human cell lines by Northern blot analysis...
  92. pmc Escherichia coli phnN, encoding ribose 1,5-bisphosphokinase activity (phosphoribosyl diphosphate forming): dual role in phosphonate degradation and NAD biosynthesis pathways
    Bjarne Hove-Jensen
    Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, Copenhagen, Denmark
    J Bacteriol 185:2793-801. 2003
    ....
  93. ncbi A new and highly sensitive assay for the ATP phosphoribosyltransferase that catalyzes the first step of histidine biosynthesis
    H M Kronenberg
    Anal Biochem 65:380-8. 1975
  94. ncbi Mammalian phosphoribosyl-pyrophosphate synthetase
    M Tatibana
    Department of Biochemistry, Chiba University School of Medicine, Japan
    Adv Enzyme Regul 35:229-49. 1995
    ..Further studies on these components and their interaction are expected to reveal various mechanisms governing mammalian PRPP synthetase...
  95. ncbi Fructose 3-phosphate and 5-phosphoribosyl-1-pyrophosphate formation in perfused human erythrocytes: 31P NMR studies
    P Lundberg
    Department of Biochemistry, University of Sydney, Australia
    Magn Reson Med 31:110-21. 1994
    ..C. 2.7.6.1) activity in the human erythrocytes and that the biosynthetic pathways are active in immobilized cells at 37 degrees C. (5) The rates of accumulation of 2,3-DPG and phosphomonoesters (PME) appeared to be strongly correlated...
  96. ncbi [PRPP synthetase deficiency]
    Kaname Kawasugi
    Department of Allergy and Rheumatology, University of Tokyo Graduate School of Medicine
    Nihon Rinsho 61:383-5. 2003
  97. ncbi Surface exposed amino acid differences between mesophilic and thermophilic phosphoribosyl diphosphate synthase
    Bjarne Hove-Jensen
    Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, Denmark
    Eur J Biochem 271:4526-33. 2004
    ..caldolyticus enzyme based on homology modelling with the structure of B. subtilis 5-phospho-alpha-D-ribosyl 1-diphosphate synthase shows 92% of the amino acid differences to be on solvent exposed surfaces in the hexameric structure...
  98. ncbi [PRPP synthetase superactivity]
    Kaname Kawasugi
    Department of Allergy and Rheumatology, University of Tokyo Graduate School of Medicine
    Nihon Rinsho 61:284-7. 2003
  99. ncbi Characterization of the hemA-prs region of the Escherichia coli and Salmonella typhimurium chromosomes: identification of two open reading frames and implications for prs expression
    D A Post
    Department of Biochemistry, University of Illinois, Urbana 61801
    J Gen Microbiol 139:259-66. 1993
    ..Derepression of the prs gene upon pyrimidine starvation was shown to be due to an increase in the amount of message originating from the promoter P2...
  100. pmc Identification of a novel p300-specific-associating protein, PRS1 (phosphoribosylpyrophosphate synthetase subunit 1)
    Atsushi Kaida
    Department of Viral Oncology, Institute for Virus Research, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan
    Biochem J 391:239-47. 2005
    ..This is the first report to demonstrate the existence of a p300 KIX-domain-specific-interacting protein that does not interact with CBP. Thus p300 may play a role in the regulation of DNA synthesis through interactions with PRS1...
  101. ncbi Regulation of energy metabolism in macrophages during hypoxia. Roles of fructose 2,6-bisphosphate and ribose 1,5-bisphosphate
    T Kawaguchi
    Department of Biochemistry, Dallas Veterans Affairs Medical Center, Dallas, Texas 75216, USA
    J Biol Chem 276:28554-61. 2001
    ..These data suggest that the activation of the above enzymes was mediated by protein kinase C acting via activation of phosphatidylinositol specific phospholipase C in the macrophages during hypoxia...