glycosyltransferases

Summary

Summary: Enzymes that catalyze the transfer of glycosyl groups to an acceptor. Most often another carbohydrate molecule acts as an acceptor, but inorganic phosphate can also act as an acceptor, such as in the case of PHOSPHORYLASES. Some of the enzymes in this group also catalyze hydrolysis, which can be regarded as transfer of a glycosyl group from the donor to water. Subclasses include the HEXOSYLTRANSFERASES; PENTOSYLTRANSFERASES; SIALYLTRANSFERASES; and those transferring other glycosyl groups. EC 2.4.

Top Publications

  1. doi Glycosyltransferases: structures, functions, and mechanisms
    L L Lairson
    Department of Chemistry, University of British Columbia, Vancouver, BC, Canada
    Annu Rev Biochem 77:521-55. 2008
  2. doi Characterization of IRX10 and IRX10-like reveals an essential role in glucuronoxylan biosynthesis in Arabidopsis
    David M Brown
    Faculty of Life Science, University of Manchester, Manchester M13 9PT, UK
    Plant J 57:732-46. 2009
  3. ncbi Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis
    David M Brown
    Faculty of Life Science, University of Manchester, Manchester M13 9PT, UK
    Plant J 52:1154-68. 2007
  4. ncbi Growth of the plant cell wall
    Daniel J Cosgrove
    Department of Biology, 208 Mueller Laboratory, Penn State University, University Park, Pennsylvania 16802, USA
    Nat Rev Mol Cell Biol 6:850-61. 2005
  5. doi The Arabidopsis IRX10 and IRX10-LIKE glycosyltransferases are critical for glucuronoxylan biosynthesis during secondary cell wall formation
    Ai Min Wu
    Department of Forest Genetics and Plant Physiology, SLU, SE 901 83 Umea, Sweden
    Plant J 57:718-31. 2009
  6. ncbi N-glucosylation of cytokinins by glycosyltransferases of Arabidopsis thaliana
    Bingkai Hou
    CNAP, Department of Biology, University of York, York YO10 5DD, United Kingdom
    J Biol Chem 279:47822-32. 2004
  7. pmc Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering
    B Storrie
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0308, USA
    J Cell Biol 143:1505-21. 1998
  8. doi Identification and characterisation of F3GT1 and F3GGT1, two glycosyltransferases responsible for anthocyanin biosynthesis in red-fleshed kiwifruit (Actinidia chinensis)
    Mirco Montefiori
    The New Zealand Institute for Plant and Food Research Ltd, Private Bag 92 169, Auckland, New Zealand
    Plant J 65:106-18. 2011
  9. ncbi Glycosyltransferases in secondary plant metabolism: tranquilizers and stimulant controllers
    P Jones
    Department of Horticulture, Viticulture and Oenology, University of Adelaide, South Australia, Australia
    Planta 213:164-74. 2001
  10. ncbi Xyloglucan endo-transglycosylase (XET) functions in gelatinous layers of tension wood fibers in poplar--a glimpse into the mechanism of the balancing act of trees
    Nobuyuki Nishikubo
    Department of Forest Genetics and Plant Physiology, Umea Plant Science Center, SLU, S901 83 Umea, Sweden
    Plant Cell Physiol 48:843-55. 2007

Detail Information

Publications279 found, 100 shown here

  1. doi Glycosyltransferases: structures, functions, and mechanisms
    L L Lairson
    Department of Chemistry, University of British Columbia, Vancouver, BC, Canada
    Annu Rev Biochem 77:521-55. 2008
    b>Glycosyltransferases catalyze glycosidic bond formation using sugar donors containing a nucleoside phosphate or a lipid phosphate leaving group...
  2. doi Characterization of IRX10 and IRX10-like reveals an essential role in glucuronoxylan biosynthesis in Arabidopsis
    David M Brown
    Faculty of Life Science, University of Manchester, Manchester M13 9PT, UK
    Plant J 57:732-46. 2009
    Xylan, the major hemicellulosic polysaccharide in Arabidopsis secondary cell walls, requires a number of glycosyltransferases (GT) to catalyse formation of the various glycosidic linkages found in the polymer...
  3. ncbi Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis
    David M Brown
    Faculty of Life Science, University of Manchester, Manchester M13 9PT, UK
    Plant J 52:1154-68. 2007
    ..All five mutants, which have mutations in genes encoding putative glycosyltransferases, exhibited large decreases in xylan...
  4. ncbi Growth of the plant cell wall
    Daniel J Cosgrove
    Department of Biology, 208 Mueller Laboratory, Penn State University, University Park, Pennsylvania 16802, USA
    Nat Rev Mol Cell Biol 6:850-61. 2005
    ..Recent discoveries have uncovered how plant cells synthesize wall polysaccharides, assemble them into a strong fibrous network and regulate wall expansion during cell growth...
  5. doi The Arabidopsis IRX10 and IRX10-LIKE glycosyltransferases are critical for glucuronoxylan biosynthesis during secondary cell wall formation
    Ai Min Wu
    Department of Forest Genetics and Plant Physiology, SLU, SE 901 83 Umea, Sweden
    Plant J 57:718-31. 2009
    ..This contrasts with the proposed function of the tobacco NpGUT1, which is closely related to the Arabidopsis IRX10 and IRX10-L proteins, in rhamnogalacturonan II biosynthesis...
  6. ncbi N-glucosylation of cytokinins by glycosyltransferases of Arabidopsis thaliana
    Bingkai Hou
    CNAP, Department of Biology, University of York, York YO10 5DD, United Kingdom
    J Biol Chem 279:47822-32. 2004
    ..This study utilizes a screening strategy in which 105 recombinant glycosyltransferases (UGTs) of Arabidopsis have been analyzed for catalytic activity toward the classical cytokinins: trans-..
  7. pmc Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering
    B Storrie
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0308, USA
    J Cell Biol 143:1505-21. 1998
    ..In conclusion, we have shown that Golgi-resident glycosylation enzymes recycle through the ER and that this novel pathway is the likely explanation for the nocodazole-induced Golgi scattering observed in interphase cells...
  8. doi Identification and characterisation of F3GT1 and F3GGT1, two glycosyltransferases responsible for anthocyanin biosynthesis in red-fleshed kiwifruit (Actinidia chinensis)
    Mirco Montefiori
    The New Zealand Institute for Plant and Food Research Ltd, Private Bag 92 169, Auckland, New Zealand
    Plant J 65:106-18. 2011
    Much of the diversity of anthocyanins is due to the action of glycosyltransferases, which add sugar moieties to anthocyanidins...
  9. ncbi Glycosyltransferases in secondary plant metabolism: tranquilizers and stimulant controllers
    P Jones
    Department of Horticulture, Viticulture and Oenology, University of Adelaide, South Australia, Australia
    Planta 213:164-74. 2001
    ..Recently, a range of glycosyltransferases has been characterized in detail with regard to substrate specificity...
  10. ncbi Xyloglucan endo-transglycosylase (XET) functions in gelatinous layers of tension wood fibers in poplar--a glimpse into the mechanism of the balancing act of trees
    Nobuyuki Nishikubo
    Department of Forest Genetics and Plant Physiology, Umea Plant Science Center, SLU, S901 83 Umea, Sweden
    Plant Cell Physiol 48:843-55. 2007
    ..Surprisingly, XTH gene products and XET activity persisted in mature G-fibers for several years, suggesting that the enzyme functions after cell death repairing the cross-links as they are being broken during the shrinking process...
  11. ncbi Glycosyltransferases and cell wall biosynthesis: novel players and insights
    Wolf Rüdiger Scheible
    Max Planck Institute for Molecular Plant Physiology, Am Muhlenberg 1, 14476 Golm, Germany
    Curr Opin Plant Biol 7:285-95. 2004
    ..Moreover, potential regulation mechanisms and associated protein factors are emerging from recent data...
  12. pmc Evolution and function of the plant cell wall synthesis-related glycosyltransferase family 8
    Yanbin Yin
    Computational Systems Biology Laboratory and Institute of Bioinformatics, University of Georgia, Athens, Georgia 30602, USA
    Plant Physiol 153:1729-46. 2010
    ....
  13. ncbi Plant glycosyltransferases
    K Keegstra
    MSU DOE Plant Research Laboratory and Department of Biochemistry, Michigan State University, 48824, East Lansing, Michigan USA
    Curr Opin Plant Biol 4:219-24. 2001
    b>Glycosyltransferases are involved in the biosyntheses of cell-wall polysaccharides, the addition of N-linked glycans to glycoproteins, and the attachment of sugar moieties to various small molecules such as hormones and flavonoids...
  14. ncbi The poplar glycosyltransferase GT47C is functionally conserved with Arabidopsis Fragile fiber8
    Gong Ke Zhou
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Plant Cell Physiol 47:1229-40. 2006
    ..Together, these results suggest that PoGT47C is functionally conserved with FRA8 and it is probably involved in xylan synthesis during wood formation...
  15. ncbi The ability of land plants to synthesize glucuronoxylans predates the evolution of tracheophytes
    Ameya R Kulkarni
    Complex Carbohydrate Research Center and US Department of Energy Bioenergy Science Center, Athens, GA 30602, USA
    Glycobiology 22:439-51. 2012
    ..patens genome contains genes with high sequence similarity to Arabidopsis CAZy family GT8, GT43 and GT47 glycosyltransferases that are likely involved in xylan synthesis...
  16. pmc Putative glycosyltransferases and other plant Golgi apparatus proteins are revealed by LOPIT proteomics
    Nino Nikolovski
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom
    Plant Physiol 160:1037-51. 2012
    ..Other newly identified proteins are likely to be involved in the transport and interconversion of nucleotide sugar substrates as well as polysaccharide and protein modification...
  17. doi Down-regulation of glycosyltransferase 8D genes in Populus trichocarpa caused reduced mechanical strength and xylan content in wood
    Quanzi Li
    Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, 840 Main Campus Drive, Raleigh, NC 27695, USA
    Tree Physiol 31:226-36. 2011
    ..These results suggest that PtrGT8Ds play key roles in xylan biosynthesis in wood. Xylan may be a more important factor than lignin affecting the stiffness and fracture strength of wood...
  18. doi An evolutionary view of functional diversity in family 1 glycosyltransferases
    Keiko Yonekura-Sakakibara
    RIKEN Plant Science Center, 1 7 22 Suehiro cho, Yokohama 230 0045, Japan
    Plant J 66:182-93. 2011
    b>Glycosyltransferases (GTs) (EC 2.4.x...
  19. doi Glycosyltransferases and their assays
    Gerd K Wagner
    School of Pharmacy, University of East Anglia, Norwich, NR4 7TJ, UK
    Chembiochem 11:1939-49. 2010
    b>Glycosyltransferases (GTs) are a large family of enzymes that are essential in all domains of life for the biosynthesis of complex carbohydrates and glycoconjugates...
  20. pmc Zebrafish models for human FKRP muscular dystrophies
    Genri Kawahara
    Division of Genetics, Program in Genetics, Harvard Medical School, Children s Hospital, Boston, MA, USA
    Hum Mol Genet 19:623-33. 2010
    ..the defective glycosylation of alpha-dystroglycan and are known to result from mutations in genes encoding glycosyltransferases. Fukutin-related protein (FKRP) was identified as a homolog of fukutin, the defective protein in Fukuyama-..
  21. doi Cellular and molecular biology of glycosphingolipid glycosylation
    Hugo J F Maccioni
    Centro de Investigaciones en Quimica Biologica de Cordoba, CIQUIBIC UNC CONICET, Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina
    J Neurochem 117:589-602. 2011
    ..oligosaccharides, particularly the mechanisms underlying the trafficking of glycosphingolipid glycosyltransferases from the endoplasmic reticulum to the Golgi, those that promote their retention in the Golgi and those that ..
  22. ncbi A molecular-capsular-type prediction system for 90 Streptococcus pneumoniae serotypes using partial cpsA-cpsB sequencing and wzy- or wzx-specific PCR
    Fanrong Kong
    Centre for Infectious Diseases and Microbiology CIDM, Institute of Clinical Pathology and Medical Research ICPMR, Westmead, NSW 2145, Australia
    J Med Microbiol 54:351-6. 2005
    ..Although further study of the relationship between MCT and CS is needed, this system now allows serotype or serogroup identification of 95 % of S. pneumoniae isolates...
  23. doi The poplar GT8E and GT8F glycosyltransferases are functional orthologs of Arabidopsis PARVUS involved in glucuronoxylan biosynthesis
    Chanhui Lee
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Plant Cell Physiol 50:1982-7. 2009
    The poplar GT8E and GT8F glycosyltransferases have previously been shown to be associated with wood formation, but their roles in the biosynthesis of wood components are not known...
  24. ncbi The PARVUS gene is expressed in cells undergoing secondary wall thickening and is essential for glucuronoxylan biosynthesis
    Chanhui Lee
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Plant Cell Physiol 48:1659-72. 2007
    ..So far, three Arabidopsis glycosyltransferases, FRAGILE FIBER8, IRREGULAR XYLEM8 and IRREGULAR XYLEM9, have been implicated in glucuronoxylan (GX) ..
  25. ncbi Molecular characterization of PoGT8D and PoGT43B, two secondary wall-associated glycosyltransferases in poplar
    Gong Ke Zhou
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Plant Cell Physiol 48:689-99. 2007
    ..Here, we report the molecular characterization of two genes, PoGT8D and PoGT43B, which encode putative glycosyltransferases, in the hybrid poplar Populus alba x tremula...
  26. pmc A class of plant glycosyltransferases involved in cellular homeostasis
    Eng Kiat Lim
    CNAP, Department of Biology, University of York, York, UK
    EMBO J 23:2915-22. 2004
    ..The glycosyltransferases involved in biotransformations of small molecules have been grouped into Family 1 of the 69 families that ..
  27. doi Congenital muscular dystrophies with defective glycosylation of dystroglycan: a population study
    E Mercuri
    Department of Child Neurology, Policlinico Gemelli, Largo Gemelli, 00168 Rome, Italy
    Neurology 72:1802-9. 2009
    Congenital muscular dystrophies (CMD) with reduced glycosylation of alpha-dystroglycan (alpha-DG) are a heterogeneous group of conditions associated with mutations in six genes encoding proven or putative glycosyltransferases.
  28. ncbi Constitutive expression of abiotic stress-inducible hot pepper CaXTH3, which encodes a xyloglucan endotransglucosylase/hydrolase homolog, improves drought and salt tolerance in transgenic Arabidopsis plants
    Seok Keun Cho
    Department of Biology, College of Science, Yonsei University, Seoul 120 749, Republic of Korea
    FEBS Lett 580:3136-44. 2006
    ....
  29. pmc Phase variation controls expression of Salmonella lipopolysaccharide modification genes by a DNA methylation-dependent mechanism
    S E Broadbent
    Centre for Immunology and Infection, Hull York Medical School and the Department of Biology, University of York, York, UK
    Mol Microbiol 77:337-53. 2010
    ..Most Salmonella gtr operons share the key regulatory elements that are identified here as essential for this epigenetic phase variation...
  30. ncbi Glycosyltransferases: managers of small molecules
    Dianna Bowles
    Centre for Novel Agricultural Products CNAP, Department of Biology, University of York, York YO10 5DD, UK
    Curr Opin Plant Biol 8:254-63. 2005
    Studies of the glycosyltransferases (GTs) of small molecules have greatly increased in recent years as new approaches have been used to identify their genes and characterize their catalytic activities...
  31. ncbi Glycosylation with heptose residues mediated by the aah gene product is essential for adherence of the AIDA-I adhesin
    I Benz
    Institut für Infektiologie Zentrum für Molekularbiologie der Entzündung ZMBE, Universitatsklinikum Munster, Germany
    Mol Microbiol 40:1403-13. 2001
    ..Consequently, the ORF A gene has been denoted 'aah' (autotransporter-adhesin-heptosyltransferase). Glycosylation by heptoses represents a novel protein modification in eubacteria...
  32. ncbi Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule
    K Nishitani
    Department of Biology, College of Liberal Arts, Kagoshima University, Japan
    J Biol Chem 267:21058-64. 1992
    ....
  33. ncbi A principal role for AtXTH18 in Arabidopsis thaliana root growth: a functional analysis using RNAi plants
    Yasue Osato
    Graduate School of Life Sciences, Tohoku University, Sendai 980 8576, Japan
    J Plant Res 119:153-62. 2006
    ....
  34. ncbi Muscular dystrophy and neuronal migration disorder caused by mutations in a glycosyltransferase, POMGnT1
    A Yoshida
    Central Laboratories for Key Technology, Kirin Brewery Co, Ltd, Kanazawa Ku, 236 0004, Yokohama, Japan
    Dev Cell 1:717-24. 2001
    ..These findings suggest that interference in O-mannosyl glycosylation is a new pathomechanism for muscular dystrophy as well as neuronal migration disorder...
  35. pmc Receptor quality control in the endoplasmic reticulum for plant innate immunity
    Yusuke Saijo
    Department of Plant Microbe Interactions, Max Planck Institute für Züchtungforschung, Koln, Germany
    EMBO J 28:3439-49. 2009
    ..Our findings suggest a critical and selective function of N-glycosylation for different layers of plant immunity, likely through quality control of membrane-localized regulators...
  36. ncbi Selectins and glycosyltransferases in leukocyte rolling in vivo
    Markus Sperandio
    University Children s Hospital Heidelberg, Division of Neonatal Medicine, University of Heidelberg, Germany
    FEBS J 273:4377-89. 2006
    ..Several glycosyltransferases, such as core 2 beta1,6-N-acetylglucosaminyltransferase-I, beta1,4-galactosyltransferases, alpha1,3-..
  37. ncbi The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature
    Jocelyn K C Rose
    Department of Plant Biology, 228 Plant Science Building, Cornell University, Ithaca, NY 14853, USA
    Plant Cell Physiol 43:1421-35. 2002
    ....
  38. doi Down-regulation of PoGT47C expression in poplar results in a reduced glucuronoxylan content and an increased wood digestibility by cellulase
    Chanhui Lee
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Plant Cell Physiol 50:1075-89. 2009
    ..Although several glycosyltransferases are implicated in glucuronoxylan (GX) biosynthesis in Arabidopsis, whether their close orthologs in woody ..
  39. ncbi XTH acts at the microfibril-matrix interface during cell elongation
    Kris Vissenberg
    University of Antwerp Drie Eiken Campus, Department of Biology, Plant Physiology and Morphology, Universiteitsplein 1, 2610 Wilrijk, Belgium
    J Exp Bot 56:673-83. 2005
    ..After incorporation of the fluorescent oligosaccharides, the xyloglucans decorate the cellulose microfibrils and become inaccessible to hydrolytic enzymes...
  40. pmc An investigation of the role of transmembrane domains in Golgi protein retention
    S Munro
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 14:4695-704. 1995
    ..These observations are more consistent with lipid-based sorting of ST TMD, but they also allow for reconciliation with the kin recognition model which appears to act on sequences outside of the TMD...
  41. pmc Predicted functions and linkage specificities of the products of the Streptococcus pneumoniae capsular biosynthetic loci
    David M Aanensen
    Department of Infectious Disease Epidemiology, Imperial College London, Room G22, Old Medical School Building, St Mary s Hospital, Norfolk Place, London W2 1PG, United Kingdom
    J Bacteriol 189:7856-76. 2007
    ..proteins, enzymes for synthesis of CPS constituents, polymerases, flippases, initial sugar transferases, glycosyltransferases [GTs], phosphotransferases, acetyltransferases, and pyruvyltransferases)...
  42. doi Sterol glycosyltransferases--the enzymes that modify sterols
    Pankaj Chaturvedi
    National Botanical Research Institute Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, 226001, Uttar Pradesh, India
    Appl Biochem Biotechnol 165:47-68. 2011
    ..Sterol glycosyltransferases (SGTs) are enzymes involved in sterol modifications and play an important role in metabolic plasticity ..
  43. pmc Bovine alpha1,3-galactosyltransferase catalytic domain structure and its relationship with ABO histo-blood group and glycosphingolipid glycosyltransferases
    L N Gastinel
    Architecture et Fonction des Macromolécules Biologiques AFMB, UMR 6098, CNRS and Universités d Aix Marseille I and II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    EMBO J 20:638-49. 2001
    ..a narrow cleft on one face, and shares a UDP-binding domain (UBD) with the recently solved inverting glycosyltransferases. The substrate-bound complex, solved and refined to 2...
  44. ncbi Identification and characterisation of Arabidopsis glycosyltransferases capable of glucosylating coniferyl aldehyde and sinapyl aldehyde
    Eng Kiat Lim
    CNAP, Department of Biology, University of York, UK
    FEBS Lett 579:2802-6. 2005
    ..UGT72E1, an UDP-glucose:glycosyltransferase of Arabidopsis thaliana that is the third member of a branch of glycosyltransferases, capable of conjugating lignin monomers and related metabolites...
  45. ncbi Glycosyltransferases involved in type 1 chain and Lewis antigen biosynthesis exhibit glycan and core chain specificity
    Jan Holgersson
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, Huddinge, S 141 86 Stockholm, Sweden
    Glycobiology 16:584-93. 2006
    ..in SLe(a), Le(a), and Le(b) synthesis, we have transiently transfected CHO-K1 cells with relevant human glycosyltransferases and, on secreted reporter proteins, detected the resulting Lewis antigens on N- and O-linked glycans using ..
  46. pmc Transcriptional regulation of proteoglycans and glycosaminoglycan chain-synthesizing glycosyltransferases by UV irradiation in cultured human dermal fibroblasts
    Jeong Eun Shin
    Department of Dermatology, Institute of Dermatological Science, Seoul National University College of Medicine, University Hospital, 101 Daehang no, Seoul, Korea
    J Korean Med Sci 26:417-24. 2011
    ..Expressions of GAG chain-synthesizing glycosyltransferases, xylosyltransferase-1, β1,3-glucuronyltransferase-1, β1,4-galactosyltransferase-2, -4, exostosin-1, ..
  47. ncbi Cloning and overexpression of glycosyltransferases that generate the lipopolysaccharide core of Rhizobium leguminosarum
    J L Kadrmas
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 273:26432-40. 1998
    ..The gene is separated by several kilobase pairs from the lpcAB cluster. All three glycosyltransferases are carried on cosmid pIJ1848, which contains at least 20 kilobase pairs of R. leguminosarum DNA...
  48. doi Discovery of new Gram-negative antivirulence drugs: structure and properties of novel E. coli WaaC inhibitors
    F Moreau
    MUTABILIS SA, 102 Avenue Gaston Roussel, 93230 Romainville, France
    Bioorg Med Chem Lett 18:4022-6. 2008
    ....
  49. ncbi Engineering Flax with the GT Family 1 Solanum sogarandinum Glycosyltransferase SsGT1 Confers Increased Resistance to Fusarium Infection
    Katarzyna Lorenc-Kukuła
    Faculty of Biotechnology, Wroclaw University, Przybyszewskiego 63 77, 51 148 Wroclaw, Poland
    J Agric Food Chem 57:6698-705. 2009
    ..It is thus suggested that introduction of SsGT1 is sufficient for engineering altered pathogen resistance in flax...
  50. pmc Biochemical characterization of the O-linked glycosylation pathway in Neisseria gonorrhoeae responsible for biosynthesis of protein glycans containing N,N'-diacetylbacillosamine
    Meredith D Hartley
    Departments of Chemistry and Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 50:4936-48. 2011
    ..In addition, the substrate specificities of the phospho-glycosyltransferase, glycosyltransferases, and oligosaccharyltransferase (OTase) were analyzed in vitro, and in most cases, these enzymes exhibited ..
  51. pmc Hpa2 required by HrpF to translocate Xanthomonas oryzae transcriptional activator-like effectors into rice for pathogenicity
    Yu rong Li
    School of Agriculture and Biology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, China
    Appl Environ Microbiol 77:3809-18. 2011
    ..These findings suggest that Hpa2 and HrpF form a complex to translocate T3S effectors into plant cells for pathogenesis in host rice...
  52. ncbi Structural analysis of the lipopolysaccharide from Pasteurella multocida genome strain Pm70 and identification of the putative lipopolysaccharide glycosyltransferases
    Frank St Michael
    Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada, K1A 0R6
    Glycobiology 15:323-33. 2005
    ..Identification of the core oligosaccharide structure enabled a search for glycosyltransferase homologs in the Pm70 genome and revealed a clustering of the genes putatively responsible for outer core oligosaccharide biosynthesis...
  53. ncbi Glycosyltransferases encoded by viruses
    Nicolas Markine-Goriaynoff
    Immunology Vaccinology B43b, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, B 4000 Liege, Belgium
    J Gen Virol 85:2741-54. 2004
    ..Viruses reach that goal either by regulating expression of host glycosyltransferases or by expressing their own glycosyltransferases...
  54. ncbi Sterol glycosyltransferases-identification of members of gene family and their role in stress in Withania somnifera
    Pankaj Chaturvedi
    Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, UP, India
    Mol Biol Rep 39:9755-64. 2012
    Sterol glycosyltransferases (SGTs) catalyze the transfer of sugar molecules to diverse sterol molecules, leading to a change in their participation in cellular metabolism...
  55. pmc Functional identification of an Arabidopsis pectin biosynthetic homogalacturonan galacturonosyltransferase
    Jason D Sterling
    Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602 4712, USA
    Proc Natl Acad Sci U S A 103:5236-41. 2006
    ..The identification of GAUT1 as a HG GalAT and of the GAUT1-related gene family provides the genetic and biochemical tools required to study the function of these genes in pectin synthesis...
  56. pmc Characterization of a metal-independent CAZy family 6 glycosyltransferase from Bacteroides ovatus
    Percy Tumbale
    Department of Basic Science, Charles E Schmidt College of Biomedical Science, Florida Atlantic University, Boca Raton, Florida 33431, USA
    J Biol Chem 284:25126-34. 2009
    ..In humans and other vertebrates, variations in the activity of glycosyltransferases of CAZy family 6 generate antigenic variation between individuals and species that facilitates resistance ..
  57. pmc The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity
    David N Bolam
    Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    Proc Natl Acad Sci U S A 104:5336-41. 2007
    ..The Streptomyces antibioticus glycosyltransferases, OleI and OleD, glycosylate and inactivate oleandomycin and diverse macrolides including erythromycin, ..
  58. ncbi IL-6 and IL-8 increase the expression of glycosyltransferases and sulfotransferases involved in the biosynthesis of sialylated and/or sulfated Lewisx epitopes in the human bronchial mucosa
    Sophie Groux-Degroote
    Unite de Glycobiologie Structurale et Fonctionnelle, UMR CNRS no 8576, Batiment C9, Universite des Sciences et Technologies de Lille, F 59655 Villeneuve d Ascq, Lille, France
    Biochem J 410:213-23. 2008
    ..In conclusion, our results indicate that IL-6 and -8 may contribute to the increased levels of sialyl-Lewis(x) and 6-sulfo-sialyl-Lewis(x) epitopes on human airway mucins from patients with CF...
  59. pmc Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis
    Keli Xu
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, 101 College St, East Tower, Rm 14 309, Toronto, Ontario, Canada M5G 1L7
    Am J Physiol Lung Cell Mol Physiol 298:L45-56. 2010
    ..In sum, these results demonstrate that Lfng functions to enhance Notch signaling in myofibroblast precursor cells and thereby to coordinate differentiation and mobilization of myofibroblasts required for alveolar septation...
  60. ncbi Exploring genomes for glycosyltransferases
    Sara Fasmer Hansen
    CERMAV CNRS, University of Grenoble, F 38041 Grenoble Cedex 9, France
    Mol Biosyst 6:1773-81. 2010
    b>Glycosyltransferases are one of the largest and most diverse enzyme groups in Nature. They catalyse the synthesis of glycosidic linkages by the transfer of a sugar residue from a donor to an acceptor substrate...
  61. doi Quorum sensing modulation of a putative glycosyltransferase gene cluster essential for Xanthomonas campestris biofilm formation
    Fei Tao
    Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673
    Environ Microbiol 12:3159-70. 2010
    ..These results provide new insight into the molecular mechanisms by which Xcc switches between planktonic growth and biofilm lifestyle...
  62. ncbi Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78
    Diego Martinez
    US DOE Joint Genome Institute, Walnut Creek, California 94598, USA
    Nat Biotechnol 22:695-700. 2004
    ..This genome provides a high quality draft sequence of a basidiomycete, a major fungal phylum that includes important plant and animal pathogens...
  63. ncbi A xyloglucan endotransglucosylase/hydrolase involves in growth of primary root and alters the deposition of cellulose in Arabidopsis
    Ya Bao Liu
    Key Lab of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China
    Planta 226:1547-60. 2007
    ....
  64. ncbi Cell adhesion in Arabidopsis thaliana is mediated by ECTOPICALLY PARTING CELLS 1--a glycosyltransferase (GT64) related to the animal exostosins
    Sunil Kumar Singh
    Department of Forest Genetics and Plant Physiology, SLU, 901 83 Umea, Sweden
    Plant J 43:384-97. 2005
    Despite the fact that several hundred glycosyltransferases have been identified from sequencing of plant genomes, the biological functions of only a handful have been established to date...
  65. ncbi Arabidopsis thaliana T-DNA mutants implicate GAUT genes in the biosynthesis of pectin and xylan in cell walls and seed testa
    Kerry H Caffall
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
    Mol Plant 2:1000-14. 2009
    ..Evidence is also presented to support a role for GAUT11 in seed mucilage expansion and in seed wall and mucilage composition...
  66. ncbi Recognition of synthetic O-methyl, epimeric, and amino analogues of the acceptor alpha-L-Fuc p-(1-->2)-beta-D-Gal p-OR by the blood-group A and B gene-specified glycosyltransferases
    T L Lowary
    Department of Chemistry, University of Alberta, Edmonton, Canada
    Carbohydr Res 251:33-67. 1994
    The disaccharide alpha-L-Fuc p-(1-->2)-beta-D-Gal p-O-(CH2)7CH3 (6) is an acceptor for the glycosyltransferases responsible for the biosynthesis of the A and B blood-group antigens...
  67. ncbi 454 EST analysis detects genes putatively involved in ginsenoside biosynthesis in Panax ginseng
    S Chen
    Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Haidian, Beijing 100193, China
    Plant Cell Rep 30:1593-601. 2011
    ..genes putatively responsible for modification of the skeletons, including 133 cytochrome P450s and 235 glycosyltransferases, were identified...
  68. pmc Xyloglucan endo-transglycosylase-mediated xyloglucan rearrangements in developing wood of hybrid aspen
    Nobuyuki Nishikubo
    Department of Forest Genetics and Plant Physiology, Umea Plant Science Center, SE 90183 Umea, Sweden
    Plant Physiol 155:399-413. 2011
    ..XET overexpression promoted vessel element growth but not fiber expansion. We suggest that the amount of nascent xyloglucan relative to XET is an important determinant of whether XET strengthens or loosens the cell wall...
  69. ncbi Biosynthesis of chondroitin sulfate: from the early, precursor discoveries to nowadays, genetics approaches
    Mauro S G Pavão
    Laboratório de Tecido Conjuntivo, Hospital Universitario Clementino Fraga Filho, Instituto de Bioquímica Médica and Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, RJ 21941 590, Brazil
    Adv Pharmacol 53:117-40. 2006
  70. ncbi Xyloglucan endotransglucosylase action is high in the root elongation zone and in the trichoblasts of all vascular plants from Selaginella to Zea mays
    K Vissenberg
    Laboratory of Plant Physiology and Morphology, Department of Biology, University of Antwerp UIA, Universiteitsplein 1, B 2610 Wilrijk, Belgium
    J Exp Bot 54:335-44. 2003
    ..Clearly XTH and its action in root development evolved before the evolutionary divergence of ferns and seed plants and also of the lycopodiophytes and euphyllophytes...
  71. doi Genetic analysis of genes controlling natural variation of seed coat and flower colors in soybean
    Kiwoung Yang
    Bio Evaluation Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon, Chungbuk 363 883, Republic of Korea
    J Hered 101:757-68. 2010
    ....
  72. ncbi Developmental expression patterns of Arabidopsis XTH genes reported by transgenes and Genevestigator
    Jaime Becnel
    Biochemistry and Cell Biology, Rice University, Houston, TX 77005 1892, USA
    Plant Mol Biol 61:451-67. 2006
    ..Knowledge of gene-specific expression among family members yields evidence of where and when gene products may function and provides insights to guide rational approaches to investigate function through reverse genetics...
  73. ncbi Characterization of three glycosyltransferases involved in the biosynthesis of the phenolic glycolipid antigens from the Mycobacterium tuberculosis complex
    Esther Perez
    Département Mécanismes Moléculaires des Infections Mycobactériennes, Institut de Pharmacologie et Biologie Structurale, CNRS and Université Paul Sabatier Unité Mixte de Recherche 5089, 205 route de Narbonne, 31077 Toulouse Cedex, France
    J Biol Chem 279:42574-83. 2004
    ..tuberculosis are grouped on a 70-kilobase chromosomal fragment containing three genes encoding putative glycosyltransferases: Rv2957, Rv2958c, and Rv2962c. To determine the functions of these genes, three recombinant M...
  74. pmc Identification of a Gene Cluster for the Biosynthesis of a Long, Galactose-Rich Exopolysaccharide in Lactobacillus rhamnosus GG and Functional Analysis of the Priming Glycosyltransferase
    Sarah Lebeer
    Centre of Microbial and Plant Genetics, K U Leuven, Kasteelpark Arenberg, Belgium
    Appl Environ Microbiol 75:3554-63. 2009
    ..rhamnosus GG, possibly because of less shielding of adhesins such as fimbria-like structures...
  75. ncbi Identification and biochemical characterization of an Arabidopsis indole-3-acetic acid glucosyltransferase
    R G Jackson
    Department of Biology, University of York, York YO10 5DD, United Kingdom
    J Biol Chem 276:4350-6. 2001
    ..This study describes the first biochemical analysis of a recombinant IAA-UGT and provides the foundation for future genetic approaches to understand the role of 1-O-indole acetyl glucose ester in Arabidopsis...
  76. ncbi LARGE can functionally bypass alpha-dystroglycan glycosylation defects in distinct congenital muscular dystrophies
    Rita Barresi
    Howard Hughes Medical Institute, Department of Physiology and Biophysics, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    Nat Med 10:696-703. 2004
    Several congenital muscular dystrophies caused by defects in known or putative glycosyltransferases are commonly associated with hypoglycosylation of alpha-dystroglycan (alpha-DG) and a marked reduction of its receptor function...
  77. ncbi Enzymic characterization of two recombinant xyloglucan endotransglucosylase/hydrolase (XTH) proteins of Arabidopsis and their effect on root growth and cell wall extension
    An Maris
    Department of Biology, University of Antwerp, Antwerpen, Belgium
    J Exp Bot 60:3959-72. 2009
    ..It is proposed that these specific XTH isoenzymes play a role in strengthening the side-walls of root-hairs and cell walls in the root differentiation zone after the completion of cell expansion...
  78. pmc Molecular analysis of three Aeromonas hydrophila AH-3 (serotype O34) lipopolysaccharide core biosynthesis gene clusters
    Natalia Jimenez
    Departamento Microbiologia, Facultad Biologia, Universidad Barcelona, Diagonal 645, 08071 Barcelona, Spain
    J Bacteriol 190:3176-84. 2008
    ..Having the functions of all genes involved with the LPS core biosynthesis and most corresponding single-gene mutants now allows experimental work on the role of the LPS core in the virulence of A. hydrophila...
  79. pmc The divergent DSL ligand Dll3 does not activate Notch signaling but cell autonomously attenuates signaling induced by other DSL ligands
    Ena Ladi
    Department of Biological Chemistry, Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA
    J Cell Biol 170:983-92. 2005
    ..Finally, together with the modulator lunatic fringe, Dll3 altered N signaling levels that were induced by other DSL ligands...
  80. doi Combination of several bioinformatics approaches for the identification of new putative glycosyltransferases in Arabidopsis
    Sara Fasmer Hansen
    University of Grenoble, F 38041 Grenoble, France
    J Proteome Res 8:743-53. 2009
    ..Among them, 20 are considered as putative glycosyltransferases that should further be investigated since known GT signatures were clearly identified.
  81. pmc De novo biosynthesis of vanillin in fission yeast (Schizosaccharomyces pombe) and baker's yeast (Saccharomyces cerevisiae)
    Esben H Hansen
    Poalis A S, DK 1870 Frederiksberg C, Denmark
    Appl Environ Microbiol 75:2765-74. 2009
    ..S. pombe yeast has not previously been metabolically engineered to produce any valuable, industrially scalable, white biotech commodity...
  82. pmc Characterization of a tomato xyloglucan endotransglycosylase gene that is down-regulated by auxin in etiolated hypocotyls
    C Catala
    Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, Athens, GA 30602 4712, USA
    Plant Physiol 127:1180-92. 2001
    ..The opposite patterns of expression and differential auxin regulation of LeXET2 and LeEXT suggest that they encode XETs with distinct roles during plant growth and development...
  83. pmc The Arabidopsis irregular xylem8 mutant is deficient in glucuronoxylan and homogalacturonan, which are essential for secondary cell wall integrity
    Staffan Persson
    Carnegie Institution, Stanford, California 94305, USA
    Plant Cell 19:237-55. 2007
    ....
  84. ncbi X-ray crystal structures of rabbit N-acetylglucosaminyltransferase I (GnT I) in complex with donor substrate analogues
    Roni D Gordon
    Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    J Mol Biol 360:67-79. 2006
    ....
  85. ncbi Xyloglucan endotransglycosylases: diversity of genes, enzymes and potential wall-modifying functions
    P Campbell
    Dept of Botany, Duke University, Durham, NC 27708 1000, USA
    Trends Plant Sci 4:361-6. 1999
    ..Xyloglucan endotransglycosylases are encoded by large gene families, and in an attempt to clarify their physiological role, the diverse regulation of the genes and properties of the proteins are being determined...
  86. pmc Emerging paradigms for the initiation of mucin-type protein O-glycosylation by the polypeptide GalNAc transferase family of glycosyltransferases
    Thomas A Gerken
    Department of Pediatrics W A Bernbaum Center for Cystic Fibrosis Research, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 286:14493-507. 2011
    ..We conclude that each ppGalNAc T isoform may be uniquely sensitive to peptide sequence and overall charge, which together dictates the substrate sites that will be glycosylated...
  87. pmc Pasteurella multocida expresses two lipopolysaccharide glycoforms simultaneously, but only a single form is required for virulence: identification of two acceptor-specific heptosyl I transferases
    Marina Harper
    Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Victoria 3800, Australia
    Infect Immun 75:3885-93. 2007
    ....
  88. pmc A novel bioinformatics approach identifies candidate genes for the synthesis and feruloylation of arabinoxylan
    Rowan A C Mitchell
    Biomathematics and Bioinformatics Division, Rothamsted Research, Harpenden, Hertfordshire, United Kingdom
    Plant Physiol 144:43-53. 2007
    ....
  89. ncbi Mechanical effects of plant cell wall enzymes on cellulose/xyloglucan composites
    Elisabeth Chanliaud
    Unilever Research Colworth, Colworth House, Sharnbrook, Bedford MK44 1LQ, UK
    Plant J 38:27-37. 2004
    ..Mechanical consequences of tXET action are shown to be complimentary to those of cucumber expansin...
  90. ncbi Engineering of glycosidases and glycosyltransferases
    Susan M Hancock
    Department of Chemistry, University of British Columbia, Vancouver, British Columbia, Canada
    Curr Opin Chem Biol 10:509-19. 2006
    In recent years, substantial advances have been made in the engineering of glycosidases and glycosyltransferases for the synthesis and degradation of glycan structures...
  91. ncbi A census of carbohydrate-active enzymes in the genome of Arabidopsis thaliana
    B Henrissat
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098, CNRS and Universités d Aix Marseille I and II, France
    Plant Mol Biol 47:55-72. 2001
    ..and functional diversity of glycosides is mirrored by a vast array of enzymes involved in their synthesis (glycosyltransferases), modification (carbohydrate esterases) and breakdown (glycoside hydrolases and polysaccharide lyases)...
  92. pmc Golgi localization of glycosyltransferases requires a Vps74p oligomer
    Karl R Schmitz
    Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Dev Cell 14:523-34. 2008
    ..All Golgi glycosyltransferases are type II membrane proteins with small cytosolic domains that contribute to Golgi localization...
  93. pmc Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity
    J Jungmann
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 17:423-34. 1998
    ..we identify two new proteins in the Anp1p-Mnn9p-containing complex which have homology to known glycosyltransferases. Both protein complexes have alpha-1, 6-mannosyltransferase activity, forming a series of poly-mannose ..
  94. doi Construction of a rice glycosyltransferase phylogenomic database and identification of rice-diverged glycosyltransferases
    Pei Jian Cao
    Department of Plant Pathology, University of California, Davis, CA 95616, USA
    Mol Plant 1:858-77. 2008
    b>Glycosyltransferases (GTs; EC 2.4.x.y) constitute a large group of enzymes that form glycosidic bonds through transfer of sugars from activated donor molecules to acceptor molecules...
  95. pmc Golgi N-glycosyltransferases form both homo- and heterodimeric enzyme complexes in live cells
    Antti Hassinen
    Department of Biochemistry, University of Oulu and the Glycoscience Graduate School Finland, PO Box 3000, Linnanmaa, FIN 90014 Oulu, Finland
    J Biol Chem 285:17771-7. 2010
    ..of this diversity between molecules, cells, and tissues is generated by Golgi-resident glycosidases and glycosyltransferases. However, their exact mode of functioning in glycan processing is currently unclear...
  96. ncbi Structural features of glycosyltransferases synthesizing major bilayer and nonbilayer-prone membrane lipids in Acholeplasma laidlawii and Streptococcus pneumoniae
    Maria Edman
    Department of Biochemistry, Umea University, Sweden
    J Biol Chem 278:8420-8. 2003
    ..used together with SWISS-MODEL to build three-dimensional model structures for three MGS and two DGS lipid glycosyltransferases. Two Escherichia coli proteins with known structures were identified as the best templates, the membrane ..
  97. pmc QUASIMODO1 encodes a putative membrane-bound glycosyltransferase required for normal pectin synthesis and cell adhesion in Arabidopsis
    Sophie Bouton
    Unité de Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique Centre de Versailles Grignon, 78026 Versailles Cedex, France
    Plant Cell 14:2577-90. 2002
    ..The mutant phenotype is consistent with a central role for pectins in cell adhesion...
  98. pmc The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI
    Changsheng Zhang
    Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, School of Pharmacy, UW National Cooperative Drug Discovery Group, Program, University of Wisconsin Madison, Madison, Wisconsin 53705 2222, USA
    Chembiochem 9:2506-14. 2008
    The overproduction, purification, and in vitro characterization of the polyene glycosyltransferases (GTs) AmphDI and NysDI are reported...
  99. doi Single amino acid mutations of Medicago glycosyltransferase UGT85H2 enhance activity and impart reversibility
    Luzia V Modolo
    Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK 73401, USA
    FEBS Lett 583:2131-5. 2009
    ..More interestingly, this single mutation (V200E) conferred reversibility in the glycosyltransfer reaction, indicating that Glu200 is a key determinant for the deglycosylation function...
  100. pmc Optimizing glycosyltransferase specificity via "hot spot" saturation mutagenesis presents a catalyst for novobiocin glycorandomization
    Gavin J Williams
    Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, School of Pharmacy, National Cooperative Drug Discovery Program, University of Wisconsin Madison, 777 Highland Avenue, Madison, WI 53705, USA
    Chem Biol 15:393-401. 2008
    ..Incredibly, even in the absence of a high-throughput screen for novobiocic acid glycosylation, this approach rapidly led to improvements in the desired catalytic activity of several hundred-fold...
  101. ncbi In vitro activities of four xyloglucan endotransglycosylases from Arabidopsis
    P Campbell
    Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005 1892, USA
    Plant J 18:371-82. 1999
    ..Subtle differences in biochemical activities may influence the physiological functions of the distinct XETs in vivo...

Research Grants83

  1. Cell wall synthesis enzymes and beta-lactam resistance in Enterococcus faecium
    Louis B Rice; Fiscal Year: 2011
    ..As a class B Pbp with only transpeptidase activity, Pbp5 must coordinate its activities with that of glycosyltransferases to synthesize mature peptidoglycan...
  2. Cell wall synthesis enzymes and beta-lactam resistance in Enterococcus faecium
    Louis B Rice; Fiscal Year: 2010
    ..As a class B Pbp with only transpeptidase activity, Pbp5 must coordinate its activities with that of glycosyltransferases to synthesize mature peptidoglycan...
  3. Investigation on Oligosaccharides as Antimicrobial and Prebiotics
    Peng George Wang; Fiscal Year: 2010
    ..the natural carbohydrate biosynthetic pathway where oligosaccharides are assembled together by specific glycosyltransferases using individual sugar nucleotides as building blocks...
  4. Investigation on Oligosaccharides as Antimicrobial and Prebiotics
    Peng Wang; Fiscal Year: 2009
    ..the natural carbohydrate biosynthetic pathway where oligosaccharides are assembled together by specific glycosyltransferases using individual sugar nucleotides as building blocks...
  5. Genetics and biosynthesis of an O antigen essential for symbiosis
    KENNETH D NOEL; Fiscal Year: 2010
    ..examination of reactions in vitro with cell extracts from the various mutants predicted to have lesions in glycosyltransferases. In particular the work seeks to define the enzymes and products of the first reaction in the synthesis of ..
  6. GLYCOSYLATION MECHANISMS OF CELL LIPIDS AND PROTEINS
    CARLOS HIRSCHBERG; Fiscal Year: 1990
    ..within the GA in vivo and to determine the relationship between the translocators and corresponding glycosyltransferases in the GA membrane. (2) Continue our studies on the mechanism of glycosylation in the RER...
  7. LIPID A MODIFICATION SYSTEMS IN GRAM-NEGATIVE BACTERIA
    CHRISTIAN RAETZ; Fiscal Year: 2009
    ..the coming grant period will be: 1) the purification and characterization of the lipid A phosphatases and glycosyltransferases of F. novicida;2) the biochemical and genetic analysis of LPS core assembly enzymes of F...
  8. LIPID A MODIFICATION SYSTEMS IN GRAM-NEGATIVE BACTERIA
    CHRISTIAN R RAETZ; Fiscal Year: 2010
    ..the coming grant period will be: 1) the purification and characterization of the lipid A phosphatases and glycosyltransferases of F. novicida;2) the biochemical and genetic analysis of LPS core assembly enzymes of F...
  9. PHOTOAFFINITY LABELING OF GLYCOSYLTRANSFERASES OF ENDOPLASMIC RETICULUM
    ANANT MENON; Fiscal Year: 2000
    ..This glycan-assembly apparatus consists of a wide spectrum of proteins (mainly glycosyltransferases (GTs)) involved in the construction of N-glycans and glycosylphosphatidylinositol (GPI) anchors...
  10. HTS assay development for inhibitors of sickle cell
    PAUL FRENETTE; Fiscal Year: 2006
    ..The synthesis of selectin ligands requires the expression of several glycosyltransferases that modify the carbohydrate composition of specific polypeptide or lipid, allowing high-affinity selectin ..
  11. HTS assay development for inhibitors of sickle cell
    PAUL FRENETTE; Fiscal Year: 2005
    ..The synthesis of selectin ligands requires the expression of several glycosyltransferases that modify the carbohydrate composition of specific polypeptide or lipid, allowing high-affinity selectin ..
  12. Lipid Translocating Glycosyltransferases in Congenital Neurological Disease
    PETER AB ORLEAN; Fiscal Year: 2010
    ..Seven mutations causing human glycosylation deficiencies occur in genes for Dol-P-sugar-utilizing glycosyltransferases (DPS-GT), unusual multi-spanning membrane proteins...
  13. Lipid Translocating Glycosyltransferases in Congenital Neurological Disease
    Peter Orlean; Fiscal Year: 2009
    ..Seven mutations causing human glycosylation deficiencies occur in genes for Dol-P-sugar-utilizing glycosyltransferases (DPS-GT), unusual multi-spanning membrane proteins...
  14. OLIGOSACCHARIDES AS ANTIINFLAMMATORY AGENTS
    Peter Ward; Fiscal Year: 1992
    ..the sialyl Lex tetrasaccharide will be synthesized using recombinant glycosyltransferases. Recombinant glycoproteins expressing sialyl Lex (L-selectin, lamp-1, and CD43/leukosialin) will also be ..
  15. A novel glycosylation-associated protein is required for fimbriae biogenesis
    Haley L Echlin; Fiscal Year: 2012
    ..An eleven gene cluster encoding glycosyltransferases and accessory Sec components is required for Fap1 biogenesis and glycosylation and includes a core region ..
  16. BREAST CANCER PROGNOSIS WITH CELLULAR MARKERS
    Roberto Ceriani; Fiscal Year: 1992
    ..We will use different markers of oligosaccharide synthesis available to include: cDNA probes for key glycosyltransferases in in situ hybridization; Abs against enzymes critical to oligosaccharide elongation (anti-mannosidase II); ..
  17. O-Glycans on Mucins at the Ocular Surface
    PABLO ARGUESO; Fiscal Year: 2005
    ..B. Identify and localize glycosyltransferases (sialyltransferases) responsible for their biosynthesis. C...
  18. O-Glycans on Mucins at the Ocular Surface
    PABLO ARGUESO; Fiscal Year: 2007
    ..B. Identify and localize glycosyltransferases (sialyltransferases) responsible for their biosynthesis. C...
  19. O-Glycans on Mucins at the Ocular Surface
    PABLO ARGUESO; Fiscal Year: 2003
    ..B. Identify and localize glycosyltransferases (sialyltransferases) responsible for their biosynthesis. C...
  20. O-Glycans on Mucins at the Ocular Surface
    PABLO ARGUESO; Fiscal Year: 2006
    ..B. Identify and localize glycosyltransferases (sialyltransferases) responsible for their biosynthesis. C...
  21. O-Glycans on Mucins at the Ocular Surface
    PABLO ARGUESO; Fiscal Year: 2004
    ..B. Identify and localize glycosyltransferases (sialyltransferases) responsible for their biosynthesis. C...
  22. Structure and function of family 1 glycosyltransferases
    R Garavito; Fiscal Year: 2005
    NDP-sugar glycosyltransferases are critical for the biosynthesis of all complex carbohydrates and glycoconjugates...
  23. Structure and function of family 1 glycosyltransferases
    R Garavito; Fiscal Year: 2004
    NDP-sugar glycosyltransferases are critical for the biosynthesis of all complex carbohydrates and glycoconjugates...
  24. Structure and function of family 1 glycosyltransferases
    R Garavito; Fiscal Year: 2006
    NDP-sugar glycosyltransferases are critical for the biosynthesis of all complex carbohydrates and glycoconjugates...
  25. 'Immunity of Schistosome Glycoconjugates'
    Richard Cummings; Fiscal Year: 2001
    ..containing the novel schistosome glycan epitopes by semi-synthetic chemistry using purified and recombinant glycosyltransferases, and conjugate these glycans to protein carriers to test their recognition by sera from infected animals ..
  26. Structure and function of family 1 glycosyltransferases
    R Garavito; Fiscal Year: 2003
    NDP-sugar glycosyltransferases are critical for the biosynthesis of all complex carbohydrates and glycoconjugates...
  27. Hereditary Multiple Exostosis Syndrome: Pathogenesis and Treatment
    Maurizio Pacifici; Fiscal Year: 2009
    ..The genes are EXT1 and EXT2 that encode Golgi-associated glycosyltransferases responsible for heparan sulfate (HS) synthesis...
  28. MECHANISMS OF POLYSACCHARIDE SYNTHESIS BY S. PNEUMONIAE
    Janet Yother; Fiscal Year: 2003
    ..The type 3 synthase is a member of a family of glycosyltransferases that includes the streptococcal and eukaryotic hyaluronan synthases, chitin synthases, and cellulose ..
  29. Hereditary Multiple Exostosis Syndrome: Pathogenesis and Treatment
    MAURIZIO contact PACIFICI; Fiscal Year: 2010
    ..The genes are EXT1 and EXT2 that encode Golgi-associated glycosyltransferases responsible for heparan sulfate (HS) synthesis...
  30. MECHANISMS OF POLYSACCHARIDE SYNTHESIS BY S. PNEUMONIAE
    Janet Yother; Fiscal Year: 2004
    ..The type 3 synthase is a member of a family of glycosyltransferases that includes the streptococcal and eukaryotic hyaluronan synthases, chitin synthases, and cellulose ..
  31. Genetic mechanisms which control hematopoietic cell migration
    GEOFFREY KANSAS; Fiscal Year: 2009
    ..for selectins are carbohydrates, and these glycans are formed by the sequential action of a number of glycosyltransferases. The terminal, regulated step in biosynthesis of all selectin ligands is mediated by the 11,3 ..
  32. MECHANISMS OF POLYSACCHARIDE SYNTHESIS BY S. PNEUMONIAE
    Janet Yother; Fiscal Year: 2002
    ..The type 3 synthase is a member of a family of glycosyltransferases that includes the streptococcal and eukaryotic hyaluronan synthases, chitin synthases, and cellulose ..
  33. MECHANISMS OF POLYSACCHARIDE SYNTHESIS BY S. PNEUMONIAE
    Janet Yother; Fiscal Year: 2005
    ..The type 3 synthase is a member of a family of glycosyltransferases that includes the streptococcal and eukaryotic hyaluronan synthases, chitin synthases, and cellulose ..
  34. GLYCOSPHINGOLIPIDS DURING MYOGENESIS IN VITRO
    KENNETH LESKAWA; Fiscal Year: 1991
    ..Direct assay of several glycosyltransferases revealed that two were maximally activated at the time of myoblast contact and membrane fusion: GM3 ..
  35. SURFACE OLIGOSACCHARIDES IN EMBRYONAL CARCINOMA CELLS
    Richard Cummings; Fiscal Year: 1990
    ..Sugar addition is determined in part by the expression of appropriate glycosyltransferases an, glycosidases...
  36. NUCLEOTIDE DONORS AFFECTING GLYCOSYLATION PATHWAYS
    Ajit Varki; Fiscal Year: 2000
    All living cells synthesize glycan chains. The process of glycosylation is catalyzed by highly specific glycosyltransferases that require specific sugar nucleotide donors...
  37. NUCLEOTIDE DONORS AFFECTING GLYCOSYLATION PATHWAYS
    Ajit Varki; Fiscal Year: 2000
    All living cells synthesize glycan chains. The process of glycosylation is catalyzed by highly specific glycosyltransferases that require specific sugar nucleotide donors...
  38. CONTROLLED EXPRESSION OF NEOGLYCANS IN ANIMAL CELLS
    David Smith; Fiscal Year: 1991
    ..The objective of this research is to understand more about the precise role of glycosyltransferases in the regulation of glycoconjugate biosynthesis in animal cells...
  39. NUCLEOTIDE DONORS AFFECTING GLYCOSYLATION PATHWAYS
    Ajit Varki; Fiscal Year: 2002
    All living cells synthesize glycan chains. The process of glycosylation is catalyzed by highly specific glycosyltransferases that require specific sugar nucleotide donors...
  40. NUCLEOTIDE DONORS AFFECTING GLYCOSYLATION PATHWAYS
    Ajit Varki; Fiscal Year: 2001
    All living cells synthesize glycan chains. The process of glycosylation is catalyzed by highly specific glycosyltransferases that require specific sugar nucleotide donors...
  41. NUCLEOTIDE DONORS AFFECTING GLYCOSYLATION PATHWAYS
    Ajit Varki; Fiscal Year: 2003
    All living cells synthesize glycan chains. The process of glycosylation is catalyzed by highly specific glycosyltransferases that require specific sugar nucleotide donors...
  42. BIOSYNTHESIS OF HUMAN PLATELET GLYCOSPHINGOLIPIDS
    CHENG TEH WANG; Fiscal Year: 1980
    ..Enzymes responsible for the glycosphingolipid synthesis, i.e., glycosyltransferases and sialoyltransferases, will be characterized...
  43. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 1990
    ..transfer, the oligosaccharide undergoes processing which requires the concern action of glycosidases at glycosyltransferases. We propose to continue our studies on the early stages of carbohydrate processing in Saccharomyces ..
  44. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 1991
    ..transfer, the oligosaccharide undergoes processing which requires the concern action of glycosidases at glycosyltransferases. We propose to continue our studies on the early stages of carbohydrate processing in Saccharomyces ..
  45. TRANSIENT GLUCOSYLATION OF GLYCOPROTEINS
    Armando Parodi; Fiscal Year: 2007
    ..Nucleoside diphosphates generated by glycosyltransferases in the secretory pathway must be converted into monophosphates to relieve inhibition of the transferring ..
  46. TRANSIENT GLUCOSYLATION OF GLYCOPROTEINS
    Armando Parodi; Fiscal Year: 2006
    ..Nucleoside diphosphates generated by glycosyltransferases in the secretory pathway must be converted into monophosphates to relieve inhibition of the transferring ..
  47. TRANSIENT GLUCOSYLATION OF GLYCOPROTEINS
    Armando Parodi; Fiscal Year: 2005
    ..Nucleoside diphosphates generated by glycosyltransferases in the secretory pathway must be converted into monophosphates to relieve inhibition of the transferring ..
  48. TRANSIENT GLUCOSYLATION OF GLYCOPROTEINS
    Armando Parodi; Fiscal Year: 2004
    ..Nucleoside diphosphates generated by glycosyltransferases in the secretory pathway must be converted into monophosphates to relieve inhibition of the transferring ..
  49. Oligosaccharide moieties of human milk glycans that inhibit pathogens
    David S Newburg; Fiscal Year: 2010
    ..These complex carbohydrate moieties are synthesized by the many glycosyltransferases in the mammary gland;those glycans with homology to cell surface glycoconjugate pathogen receptors may ..
  50. Oligosaccharide moieties of human milk glycans that inhibit pathogens
    DAVID NEWBURG; Fiscal Year: 2009
    ..These complex carbohydrate moieties are synthesized by the many glycosyltransferases in the mammary gland;those glycans with homology to cell surface glycoconjugate pathogen receptors may ..
  51. New Animal Model for Studies of mucosal Immunity and IgA Nephropathy
    Jan Novak; Fiscal Year: 2010
    ..Gal-deficient O- glycans and that this aberrancy is due to dysregulation in expression/activity of specific glycosyltransferases. These findings indicate that Gal-deficient IgA1 plays a pivotal role in the pathogenesis of IgAN...
  52. Chemical tools to understand and target Helicobacter pylori glycosylation
    Danielle H Dube; Fiscal Year: 2010
    ..Further, Hp deletion mutants lacking putative glycosyltransferases will be constructed, and their glycoprotein profile will be assessed using MOE to identify the genes ..
  53. An Untested Approach: Biomarkers for Colon Cancer
    Khushi Matta; Fiscal Year: 2007
    ..structures of cancer-associated glycans are driven by fundamental changes in the cellular expression of the glycosyltransferases involved in their biosynthesis...
  54. MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASES
    JON THORSON; Fiscal Year: 2002
    ..target (proteins, cell walls and/or antibiotics) occur due primarily to the restricted availability of glycosyltransferases and their nucleotide diphosphate sugar substrates...
  55. MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASES
    JON THORSON; Fiscal Year: 2001
    ..target (proteins, cell walls and/or antibiotics) occur due primarily to the restricted availability of glycosyltransferases and their nucleotide diphosphate sugar substrates...
  56. MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASES
    JON THORSON; Fiscal Year: 1999
    ..target (proteins, cell walls and/or antibiotics) occur due primarily to the restricted availability of glycosyltransferases and their nucleotide diphosphate sugar substrates...
  57. MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASES
    JON THORSON; Fiscal Year: 2000
    ..target (proteins, cell walls and/or antibiotics) occur due primarily to the restricted availability of glycosyltransferases and their nucleotide diphosphate sugar substrates...
  58. NEUROBIOLOGY OF SULFATED GLUCURONYL GLYCOCONJUGATES
    FIROZE JUNGALWALA; Fiscal Year: 1991
    ..Enzymes include several glycosyltransferases and glycosidases...
  59. HUMAN FUCOSYLTRANSFERASE STRUCTURE/FUNCTION ANALYSIS
    Eric Holmes; Fiscal Year: 2000
    ..This family of enzymes is an ideal system for studies of structure function relationships of glycosyltransferases. The research proposed in this application will take advantage of the multiplicity of enzyme forms and ..
  60. HUMAN FUCOSYLTRANSFERASE STRUCTURE/FUNCTION ANALYSIS
    Eric Holmes; Fiscal Year: 2001
    ..This family of enzymes is an ideal system for studies of structure function relationships of glycosyltransferases. The research proposed in this application will take advantage of the multiplicity of enzyme forms and ..
  61. HUMAN FUCOSYLTRANSFERASE STRUCTURE/FUNCTION ANALYSIS
    Eric Holmes; Fiscal Year: 1999
    ..This family of enzymes is an ideal system for studies of structure function relationships of glycosyltransferases. The research proposed in this application will take advantage of the multiplicity of enzyme forms and ..
  62. CYTOPLASMIC FUCOSYLATION
    Christopher West; Fiscal Year: 1993
    ..a model that FP21 is synthesized and accumulates in the cytoplasm, and that it is glycosylated by resident glycosyltransferases, which are synthesized there, from cytoplasmic pools of sugar nucleotides...
  63. CYTOPLASMIC FUCOSYLATION
    Christopher West; Fiscal Year: 1992
    ..a model that FP21 is synthesized and accumulates in the cytoplasm, and that it is glycosylated by resident glycosyltransferases, which are synthesized there, from cytoplasmic pools of sugar nucleotides...
  64. RECEPTOR TOPOGRAPHY IN MICROVASCULAR LEUKOCYTE ADHESION
    Ulrich von Andrian; Fiscal Year: 2005
    ..the venular topography of lymphocyte traffic molecules involved in L-selectin-mediated rolling (glycosyltransferases and sulfotransferases, subaim 1.1) and subsequent integrin-dependent arrest (ICAM-l and -2)...
  65. SYSTEMATIC STUDY OF THREE TYPES OF GLYCOSYLTRANSFERASES
    Khushi Matta; Fiscal Year: 2001
    ..Relatively little is known of the precise substrate specificities of the glycosyltransferases that can modify this moiety...
  66. SYSTEMATIC STUDY OF THREE TYPES OF GLYCOSYLTRANSFERASES
    Khushi Matta; Fiscal Year: 2005
    ..Relatively little is known of the precise substrate specificities of the glycosyltransferases that can modify this moiety...
  67. SYSTEMATIC STUDY OF THREE TYPES OF GLYCOSYLTRANSFERASES
    Khushi Matta; Fiscal Year: 2002
    ..Relatively little is known of the precise substrate specificities of the glycosyltransferases that can modify this moiety...
  68. SYSTEMATIC STUDY OF THREE TYPES OF GLYCOSYLTRANSFERASES
    Khushi Matta; Fiscal Year: 2003
    ..Relatively little is known of the precise substrate specificities of the glycosyltransferases that can modify this moiety...
  69. SYSTEMATIC STUDY OF THREE TYPES OF GLYCOSYLTRANSFERASES
    Khushi Matta; Fiscal Year: 2004
    ..Relatively little is known of the precise substrate specificities of the glycosyltransferases that can modify this moiety...
  70. RECEPTOR TOPOGRAPHY IN MICROVASCULAR LEUKOCYTE ADHESION
    Ulrich von Andrian; Fiscal Year: 2003
    ..the venular topography of lymphocyte traffic molecules involved in L-selectin-mediated rolling (glycosyltransferases and sulfotransferases, subaim 1.1) and subsequent integrin-dependent arrest (ICAM-l and -2)...
  71. RECEPTOR TOPOGRAPHY IN MICROVASCULAR LEUKOCYTE ADHESION
    Ulrich von Andrian; Fiscal Year: 2004
    ..the venular topography of lymphocyte traffic molecules involved in L-selectin-mediated rolling (glycosyltransferases and sulfotransferases, subaim 1.1) and subsequent integrin-dependent arrest (ICAM-l and -2)...
  72. The Development of a Refrigerated Platelet Product
    HANS WANDALL; Fiscal Year: 2007
    ..the enzymatic modification of platelet surface proteins can be achieved without the addition of exogenous glycosyltransferases by the simple addition of the donor substrates UDP-galactose and CMP-sialic acid...
  73. COMPLEMENT ACTIVATION BY ANTIBODY N-GLYCANS
    FRANK WAXMAN; Fiscal Year: 1999
    ..Experiments using glycosyltransferases and glycosidases to probe N-glycan accessibility supported this hypothesis...
  74. COMPLEMENT ACTIVATION BY ANTIBODY N-GLYCANS
    FRANK WAXMAN; Fiscal Year: 2001
    ..Experiments using glycosyltransferases and glycosidases to probe N-glycan accessibility supported this hypothesis...
  75. COMPLEMENT ACTIVATION BY ANTIBODY N-GLYCANS
    FRANK WAXMAN; Fiscal Year: 2000
    ..Experiments using glycosyltransferases and glycosidases to probe N-glycan accessibility supported this hypothesis...