glycosyltransferases

Summary

Summary: Enzymes that catalyze the transfer of glycosyl groups to an acceptor. Most often another carbohydrate molecule acts as an acceptor, but inorganic phosphate can also act as an acceptor, such as in the case of PHOSPHORYLASES. Some of the enzymes in this group also catalyze hydrolysis, which can be regarded as transfer of a glycosyl group from the donor to water. Subclasses include the HEXOSYLTRANSFERASES; PENTOSYLTRANSFERASES; SIALYLTRANSFERASES; and those transferring other glycosyl groups. EC 2.4.

Top Publications

  1. doi Glycosyltransferases: structures, functions, and mechanisms
    L L Lairson
    Department of Chemistry, University of British Columbia, Vancouver, BC, Canada
    Annu Rev Biochem 77:521-55. 2008
  2. pmc Two glycosyltransferases involved in anthocyanin modification delineated by transcriptome independent component analysis in Arabidopsis thaliana
    Keiko Yonekura-Sakakibara
    RIKEN Plant Science Center, 1 7 22, Suehiro cho, Tsurumi ku, Yokohama 230 0045, Japan
    Plant J 69:154-67. 2012
  3. ncbi Biosynthesis of O-antigens: genes and pathways involved in nucleotide sugar precursor synthesis and O-antigen assembly
    Gabrielle Samuel
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia
    Carbohydr Res 338:2503-19. 2003
  4. ncbi Glycosyltransferases of lipophilic small molecules
    Dianna Bowles
    Center for Novel Agricultural Products, Department of Biology, University of York, York YO10 5DD, United Kingdom
    Annu Rev Plant Biol 57:567-97. 2006
  5. pmc Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering
    B Storrie
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0308, USA
    J Cell Biol 143:1505-21. 1998
  6. pmc Discovery of Streptococcus pneumoniae serotype 6 variants with glycosyltransferases synthesizing two differing repeating units
    Melissa B Oliver
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 288:25976-85. 2013
  7. ncbi Fringe is a glycosyltransferase that modifies Notch
    D J Moloney
    Department of Biochemistry and Cell Biology, Institute for Cell and Developmental Biology, SUNY Stony Brook, New York 11794 5215, USA
    Nature 406:369-75. 2000
  8. doi Recent structures, evolution and mechanisms of glycosyltransferases
    Christelle Breton
    CERMAV CNRS, University of Grenoble 1, BP 53, 38041 Grenoble, France
    Curr Opin Struct Biol 22:540-9. 2012
  9. ncbi Growth of the plant cell wall
    Daniel J Cosgrove
    Department of Biology, 208 Mueller Laboratory, Penn State University, University Park, Pennsylvania 16802, USA
    Nat Rev Mol Cell Biol 6:850-61. 2005
  10. ncbi Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis
    David M Brown
    Faculty of Life Science, University of Manchester, Manchester M13 9PT, UK
    Plant J 52:1154-68. 2007

Detail Information

Publications329 found, 100 shown here

  1. doi Glycosyltransferases: structures, functions, and mechanisms
    L L Lairson
    Department of Chemistry, University of British Columbia, Vancouver, BC, Canada
    Annu Rev Biochem 77:521-55. 2008
    b>Glycosyltransferases catalyze glycosidic bond formation using sugar donors containing a nucleoside phosphate or a lipid phosphate leaving group...
  2. pmc Two glycosyltransferases involved in anthocyanin modification delineated by transcriptome independent component analysis in Arabidopsis thaliana
    Keiko Yonekura-Sakakibara
    RIKEN Plant Science Center, 1 7 22, Suehiro cho, Tsurumi ku, Yokohama 230 0045, Japan
    Plant J 69:154-67. 2012
    ..Two glycosyltransferases, UGT79B1 and UGT84A2 were found to cluster with anthocyanin biosynthetic genes...
  3. ncbi Biosynthesis of O-antigens: genes and pathways involved in nucleotide sugar precursor synthesis and O-antigen assembly
    Gabrielle Samuel
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia
    Carbohydr Res 338:2503-19. 2003
    ..This review is a summary of the current knowledge on these three groups of genes that comprise the O-antigen gene clusters, focusing on the most extensively studied E. coli and S. enterica gene clusters...
  4. ncbi Glycosyltransferases of lipophilic small molecules
    Dianna Bowles
    Center for Novel Agricultural Products, Department of Biology, University of York, York YO10 5DD, United Kingdom
    Annu Rev Plant Biol 57:567-97. 2006
    b>Glycosyltransferases of small molecules transfer sugars to a wide range of acceptors, from hormones and secondary metabolites to biotic and abiotic chemicals and toxins in the environment...
  5. pmc Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering
    B Storrie
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0308, USA
    J Cell Biol 143:1505-21. 1998
    ..In conclusion, we have shown that Golgi-resident glycosylation enzymes recycle through the ER and that this novel pathway is the likely explanation for the nocodazole-induced Golgi scattering observed in interphase cells...
  6. pmc Discovery of Streptococcus pneumoniae serotype 6 variants with glycosyltransferases synthesizing two differing repeating units
    Melissa B Oliver
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 288:25976-85. 2013
    ..Single amino acid changes in cps genes encoding glycosyltransferases can alter substrate specificities, permit biosynthesis of heterogeneous capsule repeating units, and result ..
  7. ncbi Fringe is a glycosyltransferase that modifies Notch
    D J Moloney
    Department of Biochemistry and Cell Biology, Institute for Cell and Developmental Biology, SUNY Stony Brook, New York 11794 5215, USA
    Nature 406:369-75. 2000
    ....
  8. doi Recent structures, evolution and mechanisms of glycosyltransferases
    Christelle Breton
    CERMAV CNRS, University of Grenoble 1, BP 53, 38041 Grenoble, France
    Curr Opin Struct Biol 22:540-9. 2012
    Cellular glycome assembly requires the coordinated action of a large number of glycosyltransferases that catalyse the transfer of a sugar residue from a donor to specific acceptor molecules...
  9. ncbi Growth of the plant cell wall
    Daniel J Cosgrove
    Department of Biology, 208 Mueller Laboratory, Penn State University, University Park, Pennsylvania 16802, USA
    Nat Rev Mol Cell Biol 6:850-61. 2005
    ..Recent discoveries have uncovered how plant cells synthesize wall polysaccharides, assemble them into a strong fibrous network and regulate wall expansion during cell growth...
  10. ncbi Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis
    David M Brown
    Faculty of Life Science, University of Manchester, Manchester M13 9PT, UK
    Plant J 52:1154-68. 2007
    ..All five mutants, which have mutations in genes encoding putative glycosyltransferases, exhibited large decreases in xylan...
  11. ncbi The putative tumor suppressors EXT1 and EXT2 are glycosyltransferases required for the biosynthesis of heparan sulfate
    T Lind
    Department of Medical Biochemistry and Microbiology, Uppsala University, The Biomedical Center, Box 575, S 751 23 Uppsala, Sweden
    J Biol Chem 273:26265-8. 1998
    ..Thus at least two members of the EXT family of tumor suppressors encode glycosyltransferases involved in the chain elongation step of HS biosynthesis.
  12. ncbi Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule
    K Nishitani
    Department of Biology, College of Liberal Arts, Kagoshima University, Japan
    J Biol Chem 267:21058-64. 1992
    ....
  13. ncbi The PARVUS gene is expressed in cells undergoing secondary wall thickening and is essential for glucuronoxylan biosynthesis
    Chanhui Lee
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Plant Cell Physiol 48:1659-72. 2007
    ..So far, three Arabidopsis glycosyltransferases, FRAGILE FIBER8, IRREGULAR XYLEM8 and IRREGULAR XYLEM9, have been implicated in glucuronoxylan (GX) ..
  14. doi Hemicelluloses
    Henrik Vibe Scheller
    Feedstocks Division, Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California 94608, USA
    Annu Rev Plant Biol 61:263-89. 2010
    ..Hemicelluloses are synthesized by glycosyltransferases located in the Golgi membranes...
  15. pmc A class of plant glycosyltransferases involved in cellular homeostasis
    Eng Kiat Lim
    CNAP, Department of Biology, University of York, York, UK
    EMBO J 23:2915-22. 2004
    ..The glycosyltransferases involved in biotransformations of small molecules have been grouped into Family 1 of the 69 families that ..
  16. ncbi Glycosyltransferases in secondary plant metabolism: tranquilizers and stimulant controllers
    P Jones
    Department of Horticulture, Viticulture and Oenology, University of Adelaide, South Australia, Australia
    Planta 213:164-74. 2001
    ..Recently, a range of glycosyltransferases has been characterized in detail with regard to substrate specificity...
  17. ncbi Glycosyltransferases and cell wall biosynthesis: novel players and insights
    Wolf Rüdiger Scheible
    Max Planck Institute for Molecular Plant Physiology, Am Muhlenberg 1, 14476 Golm, Germany
    Curr Opin Plant Biol 7:285-95. 2004
    ..Moreover, potential regulation mechanisms and associated protein factors are emerging from recent data...
  18. ncbi Xyloglucan endo-transglycosylase (XET) functions in gelatinous layers of tension wood fibers in poplar--a glimpse into the mechanism of the balancing act of trees
    Nobuyuki Nishikubo
    Department of Forest Genetics and Plant Physiology, Umea Plant Science Center, SLU, S901 83 Umea, Sweden
    Plant Cell Physiol 48:843-55. 2007
    ..Surprisingly, XTH gene products and XET activity persisted in mature G-fibers for several years, suggesting that the enzyme functions after cell death repairing the cross-links as they are being broken during the shrinking process...
  19. pmc Endoplasmic reticulum export of glycosyltransferases depends on interaction of a cytoplasmic dibasic motif with Sar1
    Claudio G Giraudo
    Centro de Investigaciones en Química Biológica de Córdoba CIQUIBIC, Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Ciudad Universitaria, 5000 Cordoba, Argentina
    Mol Biol Cell 14:3753-66. 2003
    ..Herein, we describe a third class of ER export motif, [RK](X)[RK], at the CT of Golgi resident glycosyltransferases that is required for these type II membrane proteins to exit the ER...
  20. ncbi Genomics-based selection and functional characterization of triterpene glycosyltransferases from the model legume Medicago truncatula
    Lahoucine Achnine
    Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
    Plant J 41:875-87. 2005
    ..array-based and in silico transcript profiling, and targeted metabolite profiling, to identify triterpene glycosyltransferases (GTs) from among the more than 300 GTs expressed in M. truncatula...
  21. pmc Evolution and function of the plant cell wall synthesis-related glycosyltransferase family 8
    Yanbin Yin
    Computational Systems Biology Laboratory and Institute of Bioinformatics, University of Georgia, Athens, Georgia 30602, USA
    Plant Physiol 153:1729-46. 2010
    ....
  22. doi Structure, mechanism and engineering of plant natural product glycosyltransferases
    Xiaoqiang Wang
    Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, United States
    FEBS Lett 583:3303-9. 2009
    ..Uridine diphosphate glycosyltransferases (UGTs) are the central players in these glycosylation processes for decorating natural products with sugars...
  23. ncbi Chondroitin sulfate synthase-2. Molecular cloning and characterization of a novel human glycosyltransferase homologous to chondroitin sulfate glucuronyltransferase, which has dual enzymatic activities
    Toshikazu Yada
    Institute for Molecular Science of Medicine, Aichi Medical University, Nagakute, Aichi 480 1195, Japan
    J Biol Chem 278:30235-47. 2003
    ..Sequence analysis based on three-dimensional structure revealed the presence of not typical but significant beta 4-glycosyltransferase architecture...
  24. pmc Natural-product sugar biosynthesis and enzymatic glycodiversification
    Christopher J Thibodeaux
    Division of Medicinal Chemistry, College of Pharmacy and Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA
    Angew Chem Int Ed Engl 47:9814-59. 2008
    ..Remarkably, many of these sugar biosynthetic enzymes and glycosyltransferases also exhibit relaxed substrate specificity...
  25. pmc Hpa2 required by HrpF to translocate Xanthomonas oryzae transcriptional activator-like effectors into rice for pathogenicity
    Yu rong Li
    School of Agriculture and Biology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, China
    Appl Environ Microbiol 77:3809-18. 2011
    ..These findings suggest that Hpa2 and HrpF form a complex to translocate T3S effectors into plant cells for pathogenesis in host rice...
  26. pmc Biochemical characterization of the O-linked glycosylation pathway in Neisseria gonorrhoeae responsible for biosynthesis of protein glycans containing N,N'-diacetylbacillosamine
    Meredith D Hartley
    Departments of Chemistry and Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 50:4936-48. 2011
    ..In addition, the substrate specificities of the phospho-glycosyltransferase, glycosyltransferases, and oligosaccharyltransferase (OTase) were analyzed in vitro, and in most cases, these enzymes exhibited ..
  27. pmc Chemoenzymatic synthesis of homogeneous ultralow molecular weight heparins
    Yongmei Xu
    Division of Medicinal Chemistry and Natural Products, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA
    Science 334:498-501. 2011
    ..The chemoenzymatic approach is scalable and shows promise for a more efficient route to synthesize this important class of medicinal agent...
  28. ncbi Molecular characterization of PoGT8D and PoGT43B, two secondary wall-associated glycosyltransferases in poplar
    Gong Ke Zhou
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Plant Cell Physiol 48:689-99. 2007
    ..Here, we report the molecular characterization of two genes, PoGT8D and PoGT43B, which encode putative glycosyltransferases, in the hybrid poplar Populus alba x tremula...
  29. ncbi N-glucosylation of cytokinins by glycosyltransferases of Arabidopsis thaliana
    Bingkai Hou
    CNAP, Department of Biology, University of York, York YO10 5DD, United Kingdom
    J Biol Chem 279:47822-32. 2004
    ..This study utilizes a screening strategy in which 105 recombinant glycosyltransferases (UGTs) of Arabidopsis have been analyzed for catalytic activity toward the classical cytokinins: trans-..
  30. doi Recent advances toward the inhibition of mAG and LAM synthesis in Mycobacterium tuberculosis
    Francis E Umesiri
    Department of Chemistry, University of Toledo, Toledo, Ohio 34606, USA
    Med Res Rev 30:290-326. 2010
    ..In this report, we review recent efforts to characterize the enzymes involved in mAG and LAM synthesis and describe the compounds used to inhibit the enzymes or characterize their catalytic activity...
  31. ncbi Human glycogene cloning: focus on beta 3-glycosyltransferase and beta 4-glycosyltransferase families
    Hisashi Narimatsu
    Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Open Space Laboratory, Central 2, Umezono 1 1 1, Tsukuba, Ibaraki 305 8568, Japan
    Curr Opin Struct Biol 16:567-75. 2006
    Glycogenes encode proteins involved in glycan synthesis, such as glycosyltransferases, sulfotransferases and sugar-nucleotide transporters...
  32. doi Functional characterization of two clusters of Brachypodium distachyon UDP-glycosyltransferases encoding putative deoxynivalenol detoxification genes
    Wolfgang Schweiger
    Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, A 3430 Tulln, Austria
    Mol Plant Microbe Interact 26:781-92. 2013
    Plant small-molecule UDP-glycosyltransferases (UGT) glycosylate a vast number of endogenous substances but also act in detoxification of metabolites produced by plant-pathogenic microorganisms...
  33. doi A structural motif is the recognition site for a new family of bacterial protein O-glycosyltransferases
    Marie Eve Charbonneau
    Canada Research Chair on Bacterial Animal Diseases, Universite de Montreal, Faculté de Médecine Vétérinaire St Hyacinthe, 3200 Sicotte, St Hyacinthe, Quebec, Canada
    Mol Microbiol 83:894-907. 2012
    ..Overall, our findings suggest that Aah represents the prototype of a new large family of bacterial protein O-glycosyltransferases that modify various substrates recognized through a structural motif.
  34. pmc The glycosyltransferase repertoire of the spikemoss Selaginella moellendorffii and a comparative study of its cell wall
    Jesper Harholt
    Department of Plant Biology and Biotechnology, VKR Research Centre Pro Active Plants, University of Copenhagen, Frederiksberg, Denmark
    PLoS ONE 7:e35846. 2012
    ..b>Glycosyltransferases (GTs) are involved in many aspects of a plant life, including cell wall biosynthesis, protein glycosylation,..
  35. doi An evolutionary view of functional diversity in family 1 glycosyltransferases
    Keiko Yonekura-Sakakibara
    RIKEN Plant Science Center, 1 7 22 Suehiro cho, Yokohama 230 0045, Japan
    Plant J 66:182-93. 2011
    b>Glycosyltransferases (GTs) (EC 2.4.x...
  36. doi Down-regulation of glycosyltransferase 8D genes in Populus trichocarpa caused reduced mechanical strength and xylan content in wood
    Quanzi Li
    Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, 840 Main Campus Drive, Raleigh, NC 27695, USA
    Tree Physiol 31:226-36. 2011
    ..These results suggest that PtrGT8Ds play key roles in xylan biosynthesis in wood. Xylan may be a more important factor than lignin affecting the stiffness and fracture strength of wood...
  37. ncbi Plant glycosyltransferases
    K Keegstra
    MSU DOE Plant Research Laboratory and Department of Biochemistry, Michigan State University, 48824, East Lansing, Michigan USA
    Curr Opin Plant Biol 4:219-24. 2001
    b>Glycosyltransferases are involved in the biosyntheses of cell-wall polysaccharides, the addition of N-linked glycans to glycoproteins, and the attachment of sugar moieties to various small molecules such as hormones and flavonoids...
  38. doi The ability of land plants to synthesize glucuronoxylans predates the evolution of tracheophytes
    Ameya R Kulkarni
    Complex Carbohydrate Research Center and US Department of Energy Bioenergy Science Center, Athens, GA 30602, USA
    Glycobiology 22:439-51. 2012
    ..patens genome contains genes with high sequence similarity to Arabidopsis CAZy family GT8, GT43 and GT47 glycosyltransferases that are likely involved in xylan synthesis...
  39. doi Cellular and molecular biology of glycosphingolipid glycosylation
    Hugo J F Maccioni
    Centro de Investigaciones en Quimica Biologica de Cordoba, CIQUIBIC UNC CONICET, Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina
    J Neurochem 117:589-602. 2011
    ..oligosaccharides, particularly the mechanisms underlying the trafficking of glycosphingolipid glycosyltransferases from the endoplasmic reticulum to the Golgi, those that promote their retention in the Golgi and those that ..
  40. doi Glycosyltransferases and their assays
    Gerd K Wagner
    School of Pharmacy, University of East Anglia, Norwich, NR4 7TJ, UK
    Chembiochem 11:1939-49. 2010
    b>Glycosyltransferases (GTs) are a large family of enzymes that are essential in all domains of life for the biosynthesis of complex carbohydrates and glycoconjugates...
  41. pmc Zebrafish models for human FKRP muscular dystrophies
    Genri Kawahara
    Division of Genetics, Program in Genetics, Harvard Medical School, Children s Hospital, Boston, MA, USA
    Hum Mol Genet 19:623-33. 2010
    ..the defective glycosylation of alpha-dystroglycan and are known to result from mutations in genes encoding glycosyltransferases. Fukutin-related protein (FKRP) was identified as a homolog of fukutin, the defective protein in Fukuyama-..
  42. ncbi A molecular-capsular-type prediction system for 90 Streptococcus pneumoniae serotypes using partial cpsA-cpsB sequencing and wzy- or wzx-specific PCR
    Fanrong Kong
    Centre for Infectious Diseases and Microbiology CIDM, Institute of Clinical Pathology and Medical Research ICPMR, Westmead, NSW 2145, Australia
    J Med Microbiol 54:351-6. 2005
    ..Although further study of the relationship between MCT and CS is needed, this system now allows serotype or serogroup identification of 95 % of S. pneumoniae isolates...
  43. ncbi The Mesp2 transcription factor establishes segmental borders by suppressing Notch activity
    Mitsuru Morimoto
    Division of Mammalian Development, National Institute of Genetics, Mishima, Japan
    Nature 435:354-9. 2005
    ..We propose that the oscillation of Notch activity is arrested and translated in the wavefront by Mesp2...
  44. ncbi A genetic approach to Mammalian glycan function
    John B Lowe
    Department of Pathology and Howard Hughes Medical Institute, University of Michigan, Ann Arbor, Michigan 48109, USA
    Annu Rev Biochem 72:643-91. 2003
    ..Many of these genes encode enzymes termed glycosyltransferases and glycosidases that reside in the Golgi apparatus where they play the major role in constructing the ..
  45. ncbi Bacterial glycoproteins: functions, biosynthesis and applications
    Raj K Upreti
    Biomembrane Laboratory, Industrial Toxicology Research Centre, Lucknow, India
    Proteomics 3:363-79. 2003
    ..Various possible applications of these diverse biomolecules in biotechnology, vaccine development, pharmaceutics and diagnostics are also touched upon...
  46. ncbi Identification and biochemical characterization of an Arabidopsis indole-3-acetic acid glucosyltransferase
    R G Jackson
    Department of Biology, University of York, York YO10 5DD, United Kingdom
    J Biol Chem 276:4350-6. 2001
    ..This study describes the first biochemical analysis of a recombinant IAA-UGT and provides the foundation for future genetic approaches to understand the role of 1-O-indole acetyl glucose ester in Arabidopsis...
  47. ncbi The poplar glycosyltransferase GT47C is functionally conserved with Arabidopsis Fragile fiber8
    Gong Ke Zhou
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Plant Cell Physiol 47:1229-40. 2006
    ..Together, these results suggest that PoGT47C is functionally conserved with FRA8 and it is probably involved in xylan synthesis during wood formation...
  48. ncbi Molecular cloning and expression of a human chondroitin synthase
    H Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 276:38721-6. 2001
    ..Thus, we demonstrated that analogous to human heparan sulfate polymerases, the single polypeptide chondroitin synthase possesses two glycosyltransferase activities required for chain polymerization...
  49. pmc An integrative approach to the identification of Arabidopsis and rice genes involved in xylan and secondary wall development
    Ai Oikawa
    Feedstocks Division, Joint BioEnergy Institute, Emeryville, California, USA
    PLoS ONE 5:e15481. 2010
    ....
  50. doi The poplar GT8E and GT8F glycosyltransferases are functional orthologs of Arabidopsis PARVUS involved in glucuronoxylan biosynthesis
    Chanhui Lee
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Plant Cell Physiol 50:1982-7. 2009
    The poplar GT8E and GT8F glycosyltransferases have previously been shown to be associated with wood formation, but their roles in the biosynthesis of wood components are not known...
  51. ncbi Glycosyltransferases: managers of small molecules
    Dianna Bowles
    Centre for Novel Agricultural Products CNAP, Department of Biology, University of York, York YO10 5DD, UK
    Curr Opin Plant Biol 8:254-63. 2005
    Studies of the glycosyltransferases (GTs) of small molecules have greatly increased in recent years as new approaches have been used to identify their genes and characterize their catalytic activities...
  52. doi Congenital muscular dystrophies with defective glycosylation of dystroglycan: a population study
    E Mercuri
    Department of Child Neurology, Policlinico Gemelli, Largo Gemelli, 00168 Rome, Italy
    Neurology 72:1802-9. 2009
    Congenital muscular dystrophies (CMD) with reduced glycosylation of alpha-dystroglycan (alpha-DG) are a heterogeneous group of conditions associated with mutations in six genes encoding proven or putative glycosyltransferases.
  53. pmc Phase variation controls expression of Salmonella lipopolysaccharide modification genes by a DNA methylation-dependent mechanism
    S E Broadbent
    Centre for Immunology and Infection, Hull York Medical School and the Department of Biology, University of York, York, UK
    Mol Microbiol 77:337-53. 2010
    ..Most Salmonella gtr operons share the key regulatory elements that are identified here as essential for this epigenetic phase variation...
  54. ncbi Constitutive expression of abiotic stress-inducible hot pepper CaXTH3, which encodes a xyloglucan endotransglucosylase/hydrolase homolog, improves drought and salt tolerance in transgenic Arabidopsis plants
    Seok Keun Cho
    Department of Biology, College of Science, Yonsei University, Seoul 120 749, Republic of Korea
    FEBS Lett 580:3136-44. 2006
    ....
  55. ncbi Glycosylation with heptose residues mediated by the aah gene product is essential for adherence of the AIDA-I adhesin
    I Benz
    Institut für Infektiologie Zentrum für Molekularbiologie der Entzündung ZMBE, Universitatsklinikum Munster, Germany
    Mol Microbiol 40:1403-13. 2001
    ..Consequently, the ORF A gene has been denoted 'aah' (autotransporter-adhesin-heptosyltransferase). Glycosylation by heptoses represents a novel protein modification in eubacteria...
  56. ncbi A principal role for AtXTH18 in Arabidopsis thaliana root growth: a functional analysis using RNAi plants
    Yasue Osato
    Graduate School of Life Sciences, Tohoku University, Sendai 980 8576, Japan
    J Plant Res 119:153-62. 2006
    ....
  57. doi Identification and characterisation of F3GT1 and F3GGT1, two glycosyltransferases responsible for anthocyanin biosynthesis in red-fleshed kiwifruit (Actinidia chinensis)
    Mirco Montefiori
    The New Zealand Institute for Plant and Food Research Ltd, Private Bag 92 169, Auckland, New Zealand
    Plant J 65:106-18. 2011
    Much of the diversity of anthocyanins is due to the action of glycosyltransferases, which add sugar moieties to anthocyanidins...
  58. pmc Periodic repression by the bHLH factor Hes7 is an essential mechanism for the somite segmentation clock
    Yasumasa Bessho
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Genes Dev 17:1451-6. 2003
    ..Thus, periodic repression by Hes7 protein is critical for the cyclic transcription of Hes7 and Lfng, and this negative feedback represents a molecular basis for the segmentation clock...
  59. ncbi The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature
    Jocelyn K C Rose
    Department of Plant Biology, 228 Plant Science Building, Cornell University, Ithaca, NY 14853, USA
    Plant Cell Physiol 43:1421-35. 2002
    ....
  60. pmc Carbohydrate-active enzymes from the zygomycete fungus Rhizopus oryzae: a highly specialized approach to carbohydrate degradation depicted at genome level
    Evy Battaglia
    Microbiology and Kluyver Centre for Genomics of Industrial Fermentation, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    BMC Genomics 12:38. 2011
    ..Rhizopus oryzae is a zygomycete filamentous fungus, well-known as a saprobe ubiquitous in soil and as a pathogenic/spoilage fungus, causing Rhizopus rot and mucomycoses...
  61. pmc Dynamic expression and essential functions of Hes7 in somite segmentation
    Y Bessho
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Genes Dev 15:2642-7. 2001
    ..These results indicate that Hes7 controls the cyclic expression of lunatic fringe and is essential for coordinated somite segmentation...
  62. pmc Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity
    J Jungmann
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 17:423-34. 1998
    ..we identify two new proteins in the Anp1p-Mnn9p-containing complex which have homology to known glycosyltransferases. Both protein complexes have alpha-1, 6-mannosyltransferase activity, forming a series of poly-mannose ..
  63. ncbi Xyloglucan endotransglycosylases: diversity of genes, enzymes and potential wall-modifying functions
    P Campbell
    Dept of Botany, Duke University, Durham, NC 27708 1000, USA
    Trends Plant Sci 4:361-6. 1999
    ..Xyloglucan endotransglycosylases are encoded by large gene families, and in an attempt to clarify their physiological role, the diverse regulation of the genes and properties of the proteins are being determined...
  64. ncbi Characterization of three glycosyltransferases involved in the biosynthesis of the phenolic glycolipid antigens from the Mycobacterium tuberculosis complex
    Esther Perez
    Département Mécanismes Moléculaires des Infections Mycobactériennes, Institut de Pharmacologie et Biologie Structurale, CNRS and Université Paul Sabatier Unité Mixte de Recherche 5089, 205 route de Narbonne, 31077 Toulouse Cedex, France
    J Biol Chem 279:42574-83. 2004
    ..tuberculosis are grouped on a 70-kilobase chromosomal fragment containing three genes encoding putative glycosyltransferases: Rv2957, Rv2958c, and Rv2962c. To determine the functions of these genes, three recombinant M...
  65. doi Identification and characterization of glycosyltransferases involved in the biosynthesis of soyasaponin I in Glycine max
    Masaaki Shibuya
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan
    FEBS Lett 584:2258-64. 2010
    ..Two glycosyltransferases from Glycine max, designated as GmSGT2 and GmSGT3, were identified and characterized...
  66. ncbi Muscular dystrophy and neuronal migration disorder caused by mutations in a glycosyltransferase, POMGnT1
    A Yoshida
    Central Laboratories for Key Technology, Kirin Brewery Co, Ltd, Kanazawa Ku, 236 0004, Yokohama, Japan
    Dev Cell 1:717-24. 2001
    ..These findings suggest that interference in O-mannosyl glycosylation is a new pathomechanism for muscular dystrophy as well as neuronal migration disorder...
  67. doi Down-regulation of PoGT47C expression in poplar results in a reduced glucuronoxylan content and an increased wood digestibility by cellulase
    Chanhui Lee
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Plant Cell Physiol 50:1075-89. 2009
    ..Although several glycosyltransferases are implicated in glucuronoxylan (GX) biosynthesis in Arabidopsis, whether their close orthologs in woody ..
  68. ncbi Selectins and glycosyltransferases in leukocyte rolling in vivo
    Markus Sperandio
    University Children s Hospital Heidelberg, Division of Neonatal Medicine, University of Heidelberg, Germany
    FEBS J 273:4377-89. 2006
    ..Several glycosyltransferases, such as core 2 beta1,6-N-acetylglucosaminyltransferase-I, beta1,4-galactosyltransferases, alpha1,3-..
  69. pmc Predicted functions and linkage specificities of the products of the Streptococcus pneumoniae capsular biosynthetic loci
    David M Aanensen
    Department of Infectious Disease Epidemiology, Imperial College London, Room G22, Old Medical School Building, St Mary s Hospital, Norfolk Place, London W2 1PG, United Kingdom
    J Bacteriol 189:7856-76. 2007
    ..proteins, enzymes for synthesis of CPS constituents, polymerases, flippases, initial sugar transferases, glycosyltransferases [GTs], phosphotransferases, acetyltransferases, and pyruvyltransferases)...
  70. ncbi XTH acts at the microfibril-matrix interface during cell elongation
    Kris Vissenberg
    University of Antwerp Drie Eiken Campus, Department of Biology, Plant Physiology and Morphology, Universiteitsplein 1, 2610 Wilrijk, Belgium
    J Exp Bot 56:673-83. 2005
    ..After incorporation of the fluorescent oligosaccharides, the xyloglucans decorate the cellulose microfibrils and become inaccessible to hydrolytic enzymes...
  71. pmc An investigation of the role of transmembrane domains in Golgi protein retention
    S Munro
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 14:4695-704. 1995
    ..These observations are more consistent with lipid-based sorting of ST TMD, but they also allow for reconciliation with the kin recognition model which appears to act on sequences outside of the TMD...
  72. pmc The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate
    C McCormick
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
    Proc Natl Acad Sci U S A 97:668-73. 2000
    ..These findings provide a rationale to explain how inherited mutations in either of the two EXT genes can cause loss of activity, resulting in hereditary multiple exostoses...
  73. pmc Family 6 glycosyltransferases in vertebrates and bacteria: inactivation and horizontal gene transfer may enhance mutualism between vertebrates and bacteria
    Keith Brew
    Department of Basic Science, College of Medicine, Florida Atlantic University, Boca Raton, Florida 33431, USA
    J Biol Chem 285:37121-7. 2010
    b>Glycosyltransferases (GTs) control the synthesis and structures of glycans...
  74. doi BC10, a DUF266-containing and Golgi-located type II membrane protein, is required for cell-wall biosynthesis in rice (Oryza sativa L.)
    Yihua Zhou
    State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    Plant J 57:446-62. 2009
    b>Glycosyltransferases (GTs) are one of the largest enzyme groups required for the synthesis of complex wall polysaccharides and glycoproteins in plants...
  75. ncbi Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization
    Hiroshi Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 278:23666-71. 2003
    ..Thus, the ChPF is required for chondroitin polymerizing activity of mammalian ChSy...
  76. pmc N-glycans in cell survival and death: cross-talk between glycosyltransferases
    Dipak K Banerjee
    Department of Biochemistry, School of Medicine, University of Puerto Rico, Medical Sciences Campus, San Juan, PR 00936 5067
    Biochim Biophys Acta 1820:1338-46. 2012
    ..It is expected that there is a great deal of cross-talk between the participating glycosyltransferases and any missed step would express defective N-glycans that could have fatal consequences...
  77. pmc The mitochondria-associated endoplasmic-reticulum subcompartment (MAM fraction) of rat liver contains highly active sphingolipid-specific glycosyltransferases
    Dominique Ardail
    INSERM U 189, Department of Biochemistry, Lyon Sud Medical School, B P 12, 69921 Oullins Cedex, France
    Biochem J 371:1013-9. 2003
    ..of the fraction with exogenous unlabelled G(M3), underlying the presence of other sphingolipid-specific glycosyltransferases in MAM...
  78. pmc Escherichia coli K-12 Suppressor-free Mutants Lacking Early Glycosyltransferases and Late Acyltransferases: minimal lipopolysaccharide structure and induction of envelope stress response
    Gracjana Klein
    Research Center Borstel, Leibniz Center for Medicine and Biosciences, Parkallee 22, D 23845 Borstel, Germany
    J Biol Chem 284:15369-89. 2009
    ..This suppression could be alleviated by overexpression of certain mutant msbA alleles or the single-copy chromosomal MsbA-498V variant in the vicinity of Walker-box II...
  79. pmc Biochemical activities of Streptococcus pneumoniae serotype 2 capsular glycosyltransferases and significance of suppressor mutations affecting the initiating glycosyltransferase Cps2E
    David B A James
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, USA
    J Bacteriol 195:5469-78. 2013
    ..In this study, we examined the functions of three capsular glycosyltransferases (Cps2F, Cps2G, and Cps2I) involved in serotype 2 CPS synthesis, whose deletions select for secondary ..
  80. pmc The divergent DSL ligand Dll3 does not activate Notch signaling but cell autonomously attenuates signaling induced by other DSL ligands
    Ena Ladi
    Department of Biological Chemistry, Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA
    J Cell Biol 170:983-92. 2005
    ..Finally, together with the modulator lunatic fringe, Dll3 altered N signaling levels that were induced by other DSL ligands...
  81. pmc Structure of a metal-independent bacterial glycosyltransferase that catalyzes the synthesis of histo-blood group A antigen
    Nethaji Thiyagarajan
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK
    Sci Rep 2:940. 2012
    ..HBGAs are also produced by a few prokaryotes where they are synthesized by glycosyltransferases (GTs) related to human HBGA synthases...
  82. doi Glycosyltransferases and oligosaccharyltransferases in Archaea: putative components of the N-glycosylation pathway in the third domain of life
    Hilla Magidovich
    Department of Life Sciences, Ben Gurion University, Beersheva, Israel
    FEMS Microbiol Lett 300:122-30. 2009
    ..this disparity, the following study has examined 56 available archaeal genomes with the aim of identifying glycosyltransferases and oligosaccharyltransferases, including those putatively catalyzing this post-translational processing ..
  83. pmc A general strategy for the chemoenzymatic synthesis of asymmetrically branched N-glycans
    Zhen Wang
    Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA
    Science 341:379-83. 2013
    ..were selected so that the antenna of the resulting deprotected compounds could be selectively extended by glycosyltransferases to give libraries of asymmetrical multi-antennary glycans...
  84. doi The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: genetic and biochemical evidence for the roles of two glycosyltransferases and a deacetylase
    Qingzhi Fan
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, United Kingdom
    Org Biomol Chem 6:3306-14. 2008
    ..This has allowed the in vivo analysis of the respective roles of the glycosyltransferases Neo8 and Neo15, and of the deacetylase Neo16 in neomycin biosynthesis...
  85. ncbi Expansive growth of plant cell walls
    D J Cosgrove
    Department of Biology, Pennsylvania State University, University Park 16802, USA
    Plant Physiol Biochem 38:109-24. 2000
    ..Experimental results indicate that cell wall enlargement may be regulated at many levels...
  86. ncbi Production of hybrid glycopeptide antibiotics in vitro and in Streptomyces toyocaensis
    P J Solenberg
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA
    Chem Biol 4:195-202. 1997
    ..To explore further the production of more potent glycopeptide antibiotics, we assessed whether glycosyltransferases could be used to produce hybrid compounds that contain various combinations of sugars and peptide cores.
  87. pmc Putative glycosyltransferases and other plant Golgi apparatus proteins are revealed by LOPIT proteomics
    Nino Nikolovski
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom
    Plant Physiol 160:1037-51. 2012
    ..Other newly identified proteins are likely to be involved in the transport and interconversion of nucleotide sugar substrates as well as polysaccharide and protein modification...
  88. ncbi Evolution of glycosaminoglycans and their glycosyltransferases: Implications for the extracellular matrices of animals and the capsules of pathogenic bacteria
    Paul L DeAngelis
    Department of Biochemistry and Molecular Biology, Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City 73104, USA
    Anat Rec 268:317-26. 2002
    ..Recently, glycosyltransferases that form the hyaluronan, heparin/heparan, or chondroitin backbone were identified at the molecular level...
  89. pmc Involvement of chondroitin sulfate synthase-3 (chondroitin synthase-2) in chondroitin polymerization through its interaction with chondroitin synthase-1 or chondroitin-polymerizing factor
    Tomomi Izumikawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Biochem J 403:545-52. 2007
    ..Altogether these results suggest that chondroitin polymerization is achieved by multiple combinations of ChSy-1, CSS3 and ChPF. Based on these characteristics, we have renamed CSS3 ChSy-2 (chondroitin synthase-2)...
  90. doi Expression of chondroitin/dermatan sulfate glycosyltransferases during early zebrafish development
    Beata Filipek-Gorniok
    Department of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
    Dev Dyn 242:964-75. 2013
    ..Chondroitin/dermatan sulfate (CS/DS) proteoglycans present in the extracellular matrix have important structural and regulatory functions...
  91. doi Sterol glycosyltransferases--the enzymes that modify sterols
    Pankaj Chaturvedi
    National Botanical Research Institute Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, 226001, Uttar Pradesh, India
    Appl Biochem Biotechnol 165:47-68. 2011
    ..Sterol glycosyltransferases (SGTs) are enzymes involved in sterol modifications and play an important role in metabolic plasticity ..
  92. ncbi Glycosyltransferases involved in type 1 chain and Lewis antigen biosynthesis exhibit glycan and core chain specificity
    Jan Holgersson
    Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, Huddinge, S 141 86 Stockholm, Sweden
    Glycobiology 16:584-93. 2006
    ..in SLe(a), Le(a), and Le(b) synthesis, we have transiently transfected CHO-K1 cells with relevant human glycosyltransferases and, on secreted reporter proteins, detected the resulting Lewis antigens on N- and O-linked glycans using ..
  93. ncbi Identification and characterisation of Arabidopsis glycosyltransferases capable of glucosylating coniferyl aldehyde and sinapyl aldehyde
    Eng Kiat Lim
    CNAP, Department of Biology, University of York, UK
    FEBS Lett 579:2802-6. 2005
    ..UGT72E1, an UDP-glucose:glycosyltransferase of Arabidopsis thaliana that is the third member of a branch of glycosyltransferases, capable of conjugating lignin monomers and related metabolites...
  94. ncbi Cloning and overexpression of glycosyltransferases that generate the lipopolysaccharide core of Rhizobium leguminosarum
    J L Kadrmas
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 273:26432-40. 1998
    ..The gene is separated by several kilobase pairs from the lpcAB cluster. All three glycosyltransferases are carried on cosmid pIJ1848, which contains at least 20 kilobase pairs of R. leguminosarum DNA...
  95. pmc Bovine alpha1,3-galactosyltransferase catalytic domain structure and its relationship with ABO histo-blood group and glycosphingolipid glycosyltransferases
    L N Gastinel
    Architecture et Fonction des Macromolécules Biologiques AFMB, UMR 6098, CNRS and Universités d Aix Marseille I and II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    EMBO J 20:638-49. 2001
    ..a narrow cleft on one face, and shares a UDP-binding domain (UBD) with the recently solved inverting glycosyltransferases. The substrate-bound complex, solved and refined to 2...
  96. pmc Transcriptional regulation of proteoglycans and glycosaminoglycan chain-synthesizing glycosyltransferases by UV irradiation in cultured human dermal fibroblasts
    Jeong Eun Shin
    Department of Dermatology, Institute of Dermatological Science, Seoul National University College of Medicine, University Hospital, 101 Daehang no, Seoul, Korea
    J Korean Med Sci 26:417-24. 2011
    ..Expressions of GAG chain-synthesizing glycosyltransferases, xylosyltransferase-1, β1,3-glucuronyltransferase-1, β1,4-galactosyltransferase-2, -4, exostosin-1, ..
  97. pmc Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis
    Keli Xu
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, 101 College St, East Tower, Rm 14 309, Toronto, Ontario, Canada M5G 1L7
    Am J Physiol Lung Cell Mol Physiol 298:L45-56. 2010
    ..In sum, these results demonstrate that Lfng functions to enhance Notch signaling in myofibroblast precursor cells and thereby to coordinate differentiation and mobilization of myofibroblasts required for alveolar septation...
  98. doi Discovery of new Gram-negative antivirulence drugs: structure and properties of novel E. coli WaaC inhibitors
    F Moreau
    MUTABILIS SA, 102 Avenue Gaston Roussel, 93230 Romainville, France
    Bioorg Med Chem Lett 18:4022-6. 2008
    ....
  99. ncbi Structural analysis of the lipopolysaccharide from Pasteurella multocida genome strain Pm70 and identification of the putative lipopolysaccharide glycosyltransferases
    Frank St Michael
    Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada, K1A 0R6
    Glycobiology 15:323-33. 2005
    ..Identification of the core oligosaccharide structure enabled a search for glycosyltransferase homologs in the Pm70 genome and revealed a clustering of the genes putatively responsible for outer core oligosaccharide biosynthesis...
  100. pmc Characterization of a metal-independent CAZy family 6 glycosyltransferase from Bacteroides ovatus
    Percy Tumbale
    Department of Basic Science, Charles E Schmidt College of Biomedical Science, Florida Atlantic University, Boca Raton, Florida 33431, USA
    J Biol Chem 284:25126-34. 2009
    ..In humans and other vertebrates, variations in the activity of glycosyltransferases of CAZy family 6 generate antigenic variation between individuals and species that facilitates resistance ..
  101. ncbi Glycosyltransferases encoded by viruses
    Nicolas Markine-Goriaynoff
    Immunology Vaccinology B43b, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, B 4000 Liege, Belgium
    J Gen Virol 85:2741-54. 2004
    ..Viruses reach that goal either by regulating expression of host glycosyltransferases or by expressing their own glycosyltransferases...

Research Grants66

  1. Investigation on Oligosaccharides as Antimicrobial and Prebiotics
    Peng George Wang; Fiscal Year: 2013
    ..the natural carbohydrate biosynthetic pathway where oligosaccharides are assembled together by specific glycosyltransferases using individual sugar nucleotides as building blocks...
  2. LIPID A MODIFICATION SYSTEMS IN GRAM-NEGATIVE BACTERIA
    Pei Zhou; Fiscal Year: 2012
    ..the coming grant period will be: 1) the purification and characterization of the lipid A phosphatases and glycosyltransferases of F. novicida;2) the biochemical and genetic analysis of LPS core assembly enzymes of F...
  3. GENETICS AND VIRULENCE OF PNEUMOCOCCAL CAPSULAR TYPES
    JANET L YOTHER; Fiscal Year: 2012
    ..the functions of the initiating glycosyltransferase Cps2E and the effects of cps2E mutations;2) identify glycosyltransferases catalyzing type 2 repeat unit synthesis;3) characterize mechanisms involved in retention of the capsule on ..
  4. Biology of the HMW1 and HMW2 Adhesins of H. Influenzae
    JOSEPH W ST. GEME; Fiscal Year: 2013
    ..Finally, we will characterize the HMW1C-like family of glycosyltransferases. From a practical perspective, the results of these studies may be directly relevant to the development of ..
  5. Cell wall synthesis enzymes and beta-lactam resistance in Enterococcus faecium
    Louis B Rice; Fiscal Year: 2013
    ..As a class B Pbp with only transpeptidase activity, Pbp5 must coordinate its activities with that of glycosyltransferases to synthesize mature peptidoglycan...
  6. Lipid Translocating Glycosyltransferases in Congenital Neurological Disease
    PETER AB ORLEAN; Fiscal Year: 2010
    ..Seven mutations causing human glycosylation deficiencies occur in genes for Dol-P-sugar-utilizing glycosyltransferases (DPS-GT), unusual multi-spanning membrane proteins...
  7. Glycosyltransferase Golgi Retention Mechanism
    Pi Wan Cheng; Fiscal Year: 2013
    ..Mucin-type glycans are elaborated in a stepwise manner from ser/thr as catalyzed by glycosyltransferases. Among them, branching enzymes are unique because they synthesize 26 N-acetylglucosaminide branch ..
  8. Oligosaccharide moieties of human milk glycans that inhibit pathogens
    David S Newburg; Fiscal Year: 2013
    ..These complex carbohydrate moieties are synthesized by the many glycosyltransferases in the mammary gland;those glycans with homology to cell surface glycoconjugate pathogen receptors may ..
  9. Systems Biology of Glycosylation
    Sriram Neelamegham; Fiscal Year: 2013
    ..2) To quantify the role of selected glycosyltransferases and the peptide backbone in regulating O-linked glycosylation and leukocyte selecting-binding function...
  10. Role of mucin-type O-glycosylation in Trypanosoma cruzi biology
    Christopher M West; Fiscal Year: 2012
    ..This project is focused on basic questions about the biochemical and cellular functions of the putative glycosyltransferases, but future work is expected to investigate their roles in virulence in pursuit of new drug targets for ..
  11. Hereditary Multiple Exostosis Syndrome: Pathogenesis and Treatment
    MAURIZIO contact PACIFICI; Fiscal Year: 2010
    ..The genes are EXT1 and EXT2 that encode Golgi-associated glycosyltransferases responsible for heparan sulfate (HS) synthesis...
  12. Mechanisms of Mycoplasmal Disease Pathogenesis
    KEVIN F DYBVIG; Fiscal Year: 2013
    ..Thus, these genes may code for novel glycosyltransferases that are transporters only in the sense that they export the growing polysaccharide chain during synthesis...
  13. PtdIns 4-Kinase Regulation of Protein Sorting in the Golgi Apparatus
    Kathryn M Ferguson; Fiscal Year: 2013
    ..The ordered addition of carbohydrate moieties to biosynthetic cargo in the Golgi is carried out by glycosyltransferases that function sequentially, such that the products of early acting enzymes are substrates for later-acting ..
  14. Carbohydrate Antigenic Biomarkers for Epithelial Cancers
    Minoru Fukuda; Fiscal Year: 2013
    ..The second is cell transfection of specific glycosyltransferases and other glyco-modifying enzymes such as sulfotransferases, an approach that has recently met with ..
  15. Structure and function of icosahedral viruses
    Michael G Rossmann; Fiscal Year: 2013
    ..studies of the major capsid proteins of these viruses as well as specific viral proteins such as the unique glycosyltransferases that modify the viral surface proteins essential for virus assembly and interaction of the virus with its ..
  16. High-throughput genetic & small-molecule screening for therapeutic modifiers
    Peter G Schultz; Fiscal Year: 2010
    ..Causative mutations for these disorders are found in known or putative glycosyltransferases that participate in the O-glycosylation of alpha- dystroglycan, a modificaiton required for functionality...
  17. DNA REPLICATION AND GENE EXPRESSION OF CHLORELLA VIRUSES
    James Van Etten; Fiscal Year: 2009
    ..In addition to characterizing the seven putative virus-encoded glycosyltransferases, the structures of the six glycans attached to the major capsid protein will be determined...
  18. Chimeric IgG/A Tumor Immunotherapy
    KEITH WYCOFF; Fiscal Year: 2013
    ..By co-expressing our recombinant antibodies along with specific glycosyltransferases in a transgenic Nicotiana benthamiana background we will produce antibodies with N-glycan structures that ..
  19. A novel glycosylation-associated protein is required for fimbriae biogenesis
    Haley L Echlin; Fiscal Year: 2013
    ..An eleven gene cluster encoding glycosyltransferases and accessory Sec components is required for Fap1 biogenesis and glycosylation and includes a core region ..
  20. Training in structural biology &glycobiology
    Hui Wu; Fiscal Year: 2012
    ..glycosylation of Fap1 is initiated by a two enzyme heterodimeric complex that is composed by two putative glycosyltransferases Gtf1 and Gtf2. The Gtf complex catalyzes the transfer of GlcNAc to the serine-rich sites of Fap1. Dr...
  21. Biosynthesis of Polysaccharides
    Peng George Wang; Fiscal Year: 2012
    ..repeating oligosaccharide unit is synthesized in the cytoplasm by the sequential action of specific glycosyltransferases. The repeating unit is then transported to the periplasmic side of the membrane where it is polymerized ..
  22. Mechanism and Inhibition of Bacterial Transglycosylases and Transpeptidases.
    Suzanne Walker; Fiscal Year: 2013
    ..PG) biosynthesis, which involve the polymerization of disaccharide-peptide subunits by peptidoglycan glycosyltransferases (PGTs) and the crosslinking of the polymerized chains by transpeptidases (TPs)...
  23. Targeting and Function of Golgi Membrane Proteins
    Carolyn E Machamer; Fiscal Year: 2011
    ..complex is oligosaccharide processing, which is precisely controlled by expression and localization of glycosyltransferases and glycosidases...
  24. Heparan sulfate-dependent mechanisms of skeletogenesis
    Julianne Huegel; Fiscal Year: 2012
    ..HME is caused by heterozygous loss-of- function mutations in EXT1 and EXT2 that encode glycosyltransferases responsible for HS synthesis...
  25. Mycoplasma Polysaccharides and Control of Infection
    KEVIN F DYBVIG; Fiscal Year: 2012
    ..The goal is to identify the glycosyltransferases and substrates used for polysaccharide synthesis...
  26. Impact of glycosylation on PsrP function
    Anel Lizcano; Fiscal Year: 2013
    ..pathogenicity island which encodes the adhesin pneumococcal serine-rich repeat protein (PsrP), 10 putative glycosyltransferases, and 7 accessory components of an alternate secretion (Sec) system...
  27. Metabolic Engineering with Bioorthogonal Chemical Reporters
    Carolyn R Bertozzi; Fiscal Year: 2013
    ..In addition, we will perturb the expression of certain glycosyltransferases and monitor concomitant changes in the glycome by in vivo imaging...
  28. Aberrant N-Glycan Standards for Mass Spectrometry and Microarray Analysis
    ANTHONY PRUDDEN; Fiscal Year: 2013
    ..The scope of chemoenzymatic synthesis is further limited by a lack of readily available glycosyltransferases. The aim of this project is to develop a novel chemoenzymatic methodology that will give easy access to ..
  29. Pathogenic Mechanisms in Hereditary Multiple Exostoses Syndrome
    Maurizio Pacifici; Fiscal Year: 2013
    ..The genes responsible for HME cases are EXT1 and EXT2 that encode glycosyltransferases responsible for heparan sulfate (HS) synthesis...
  30. Glycobiological Analysis of Plasmodium-Vector Host Interactions
    RHOEL DAVID RAMOS DINGLASAN; Fiscal Year: 2010
    ..Cellular glycosyltransferases are involved in posttranslational and co-translation modification of proteins...
  31. Glycosylation and Function of an Oral Streptococcal Adhesion
    Hui Wu; Fiscal Year: 2013
    ..Biogenesis of Fap1 is controlled by a gene cluster encoding glycosyltransferases and accessory secretion proteins...
  32. Chemical tools to understand and target Helicobacter pylori glycosylation
    Danielle H Dube; Fiscal Year: 2010
    ..Further, Hp deletion mutants lacking putative glycosyltransferases will be constructed, and their glycoprotein profile will be assessed using MOE to identify the genes ..
  33. ST6Gal I sialyltransferase in hematopoiesis
    Joseph T Y Lau; Fiscal Year: 2013
    ..The data point to the novel concept that extracellular glycosyltransferases generated from distal sources can function as "systemic factors" in regulating hematopoiesis, putatively by ..
  34. Mechanisms of polysacchairde synthesis by S. pneumoniae
    JANET L YOTHER; Fiscal Year: 2010
    ..mechanism, and its analysis serves as a paradigm for polysaccharide synthesis by processive beta-glycosyltransferases in both prokaryotic and eukaryotic organisms...
  35. HTS for Enhancer of Glycosylation of Alpha Dystroglycan
    Xiaohua Wu; Fiscal Year: 2010
    ..Increasing evidence suggests that over-expression of glycosyltransferases enhancing the glycosylation of 1-DG can restore the functions of 1-DG in MD mouse models...
  36. Chemoenzymatic synthesis of pradimicin analogues for novel antifungal and antivir
    Jixun Zhan; Fiscal Year: 2011
    ..can be reconstituted and engineered in a heterologous host;2) Foreign sugar moieties and the corresponding glycosyltransferases can be engineered into the pradimicin biosynthetic pathway;and 3) New pradimicin analogs such as those with ..
  37. MUCIN SITE SPECIFIC O-GLYCOSYLATION
    Thomas A Gerken; Fiscal Year: 2012
    ..PUBLIC HEALTH RELEVANCE: Mutations in a series of glycosyltransferases (protein glycosylating enzymes) have recently been found in patients with colon cancer...
  38. Genetics and biosynthesis of an O antigen essential for symbiosis
    KENNETH D NOEL; Fiscal Year: 2010
    ..examination of reactions in vitro with cell extracts from the various mutants predicted to have lesions in glycosyltransferases. In particular the work seeks to define the enzymes and products of the first reaction in the synthesis of ..
  39. Autoantibodies Against Glycopeptide Epitopes as Serum Biomarkers of Cancer
    Michael A Hollingsworth; Fiscal Year: 2011
    ..Specific Aim 3. Characterize expression of tumor associated glycans and glycosyltransferases in metastatic pancreatic cancer on a series of cases in which we have primary tumor and multiple metastatic ..
  40. Establishing the Molecular Basis of Glycoconjugate Glycosylation
    James Prestegard; Fiscal Year: 2013
    ..This is no small task as there are an estimated 7000 distinct glycans in mammalian systems and ~200 glycosyltransferases (GTs) contributing to their synthesis in humans...
  41. Biosynthesis of the Immunomodulatory Molecule Capsular Polysaccharide A
    Jerry M Troutman; Fiscal Year: 2012
    ..Gene locus analysis has identified proteins homologous to four glycosyltransferases, a galactopyranose mutase, and a sugar aminotransferase, a protein responsible for pyruvation, a flippase ..
  42. Roles of Glycoslyation in Notch Signaling
    PAMELA M STANLEY; Fiscal Year: 2013
    ..Specific Aim 2 will identify individual and complementary functions of Lfng, Mfng and Rfng glycosyltransferases that transfer N-acetylglucosamine (GlcNAc) to O-fucose on Notch, and thereby regulate Notch signaling...
  43. HTS assay development for inhibitors of sickle cell
    PAUL FRENETTE; Fiscal Year: 2006
    ..The synthesis of selectin ligands requires the expression of several glycosyltransferases that modify the carbohydrate composition of specific polypeptide or lipid, allowing high-affinity selectin ..
  44. Host glycosyltransferases in the glycosylation of Toxoplasma proteins
    Anthony P Sinai; Fiscal Year: 2013
    ....
  45. Cosmc - A Novel Molecular Chaperone Regulating O-Glycans
    Richard Cummings; Fiscal Year: 2009
    ..Aim 2 - We will define whether Cosmc assists other glycosyltransferases and define its potential interaction with T-synthase and/or other proteins (e.g. chaperones) and ATP...
  46. Uncovering the controlling mechanisms in heparan sulfate biosynthesis
    Jian Liu; Fiscal Year: 2013
    ..HS biosynthesis involves a series of specialized HS sulfotransferases, glycosyltransferases and an epimerase...
  47. 'Immunity of Schistosome Glycoconjugates'
    Richard Cummings; Fiscal Year: 2001
    ..containing the novel schistosome glycan epitopes by semi-synthetic chemistry using purified and recombinant glycosyltransferases, and conjugate these glycans to protein carriers to test their recognition by sera from infected animals ..
  48. SBIR TOPIC321:CHEMICALLY DEFINED GLYCAN LIBRARIES FOR REFERENCE STANDARDS AND GLY
    Baolin Wu; Fiscal Year: 2013
    ..The N-glycans contain 5 to 11 monosaccharide residues. This process involves several well characterized glycosyltransferases, glycoside hydrolases and other enzymes...
  49. NUCLEOTIDE DONORS AFFECTING GLYCOSYLATION PATHWAYS
    Ajit Varki; Fiscal Year: 2003
    All living cells synthesize glycan chains. The process of glycosylation is catalyzed by highly specific glycosyltransferases that require specific sugar nucleotide donors...
  50. BIOSYNTHESIS OF HUMAN PLATELET GLYCOSPHINGOLIPIDS
    CHENG TEH WANG; Fiscal Year: 1980
    ..Enzymes responsible for the glycosphingolipid synthesis, i.e., glycosyltransferases and sialoyltransferases, will be characterized...
  51. Structure and function of family 1 glycosyltransferases
    R Garavito; Fiscal Year: 2006
    NDP-sugar glycosyltransferases are critical for the biosynthesis of all complex carbohydrates and glycoconjugates...
  52. BOTTOM-UP GLYCOENGINEERING LIBRARY OF N-GLYCANS
    BRIAN HAMILTON; Fiscal Year: 2013
    ..Escherichia coli to synthesize the building blocks for many human glycans, and further modify them with glycosyltransferases in vitro...
  53. MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASES
    JON THORSON; Fiscal Year: 2002
    ..target (proteins, cell walls and/or antibiotics) occur due primarily to the restricted availability of glycosyltransferases and their nucleotide diphosphate sugar substrates...
  54. Genetic mechanisms which control hematopoietic cell migration
    GEOFFREY KANSAS; Fiscal Year: 2009
    ..for selectins are carbohydrates, and these glycans are formed by the sequential action of a number of glycosyltransferases. The terminal, regulated step in biosynthesis of all selectin ligands is mediated by the 11,3 ..
  55. SYSTEMATIC STUDY OF THREE TYPES OF GLYCOSYLTRANSFERASES
    Khushi Matta; Fiscal Year: 2005
    ..Relatively little is known of the precise substrate specificities of the glycosyltransferases that can modify this moiety...
  56. RECEPTOR TOPOGRAPHY IN MICROVASCULAR LEUKOCYTE ADHESION
    Ulrich von Andrian; Fiscal Year: 2005
    ..the venular topography of lymphocyte traffic molecules involved in L-selectin-mediated rolling (glycosyltransferases and sulfotransferases, subaim 1.1) and subsequent integrin-dependent arrest (ICAM-l and -2)...
  57. The Development of a Refrigerated Platelet Product
    HANS WANDALL; Fiscal Year: 2007
    ..the enzymatic modification of platelet surface proteins can be achieved without the addition of exogenous glycosyltransferases by the simple addition of the donor substrates UDP-galactose and CMP-sialic acid...
  58. COMPLEMENT ACTIVATION BY ANTIBODY N-GLYCANS
    FRANK WAXMAN; Fiscal Year: 2001
    ..Experiments using glycosyltransferases and glycosidases to probe N-glycan accessibility supported this hypothesis...
  59. REGULATION OF GLYCOPROTEIN BIOSYNTHESIS IN LUNG
    Joseph Mendicino; Fiscal Year: 1991
    ..structure of these oligosaccharide chains are ultimately determined by the activity of a series of specific glycosyltransferases. The mechanisms which regulate the final number and structure of different oligosaccharide chains in mucin ..
  60. HUMAN FUCOSYLTRANSFERASE STRUCTURE/FUNCTION ANALYSIS
    Eric Holmes; Fiscal Year: 2001
    ..This family of enzymes is an ideal system for studies of structure function relationships of glycosyltransferases. The research proposed in this application will take advantage of the multiplicity of enzyme forms and ..
  61. Oligosaccharides Against Enteric Infections
    Ryan Woodyer; Fiscal Year: 2010
    ..oligosaccharides and analogs in sufficient quantities for preclinical and clinical testing is using glycosyltransferases in vitro with simple starting materials such as monosaccharides or readily available polysaccharides and ..
  62. MOUSE GALTRANSFERASE--STRUCTURE/EXPRESSION
    JOEL SHAPER; Fiscal Year: 2004
    ..are biosynthesized by the coordinate action of a functional family of membrane-bound enzymes, termed glycosyltransferases. In spite of the diverse role that glycans serve in the proper functioning of a cell, there is a paucity of ..
  63. GLYCOSYLATION MUTANTS OF ANIMAL CELLS
    Pamela Stanley; Fiscal Year: 1999
    ..glycosylation patterns, and to use the mutants in both functional studies and to clone genes encoding glycosyltransferases by complementation...
  64. Control T helper (Th1) cell homing
    GEOFFREY KANSAS; Fiscal Year: 2006
    ..We have shown that IL- 12 is important for the induction of certain glycosyltransferases (GT) that are crucial for the formation of selectin ligands in Th1 cells...
  65. Protein Glycosylatin in Haemophilus ducreyi
    Robert Munson; Fiscal Year: 2007
    ..ducreyi infection are poorly understood. We previously characterized many of the genes encoding glycosyltransferases responsible for synthesis of the major glycoforms of H. ducreyi lipooligosaccharide (LOS)...
  66. GLYCOSYLTRANSFERASE GENE EXPRESSION IN TRANSFORMED CELLS
    Nevis Fregien; Fiscal Year: 1992
    ..The addition of these branches is performed by the sequential action of specific glycosyltransferases. The regulation and expression of the specific glycosyltransferases...