cysteine synthase

Summary

Summary: An enzyme that catalyzes the biosynthesis of cysteine in microorganisms and plants from O-acetyl-L-serine and hydrogen sulfide. This enzyme was formerly listed as EC 4.2.99.8.

Top Publications

  1. pmc Characterization of a novel thermostable O-acetylserine sulfhydrylase from Aeropyrum pernix K1
    Koshiki Mino
    Special Division for Human Life Technology, National Institute of Advanced Industrial Science and Technology AIST, Kansai, Ikeda, Osaka 563 8577, Japan
    J Bacteriol 185:2277-84. 2003
  2. pmc Structural and biochemical studies of serine acetyltransferase reveal why the parasite Entamoeba histolytica cannot form a cysteine synthase complex
    Sudhir Kumar
    School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
    J Biol Chem 286:12533-41. 2011
  3. pmc Identification and functional analysis of Escherichia coli cysteine desulfhydrases
    Naoki Awano
    Department of Bioscience, Fukui Prefectural University, 4 1 1 Kenjojima, Matsuoka cho, Fukui 910 1195, Japan
    Appl Environ Microbiol 71:4149-52. 2005
  4. pmc A two-step process controls the formation of the bienzyme cysteine synthase complex
    Enea Salsi
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Parma, 43100 Parma, Italy
    J Biol Chem 285:12813-22. 2010
  5. ncbi Use of biomolecular interaction analysis to elucidate the regulatory mechanism of the cysteine synthase complex from Arabidopsis thaliana
    Oliver Berkowitz
    Institute for Plant Genetics and Crop Plant Research IPK, Dept Molecular Cell Biology, Corrensstrasse 3, 06466 Gatersleben, Germany
    J Biol Chem 277:30629-34. 2002
  6. doi Immunological responses to recombinant cysteine synthase A of Brucella abortus in BALB/c mice
    Shikha Jain
    Division of Microbiology, Defence Research and Development Establishment, Jhansi Road, Gwalior, 474 002 MP, India
    World J Microbiol Biotechnol 29:907-13. 2013
  7. ncbi Interactions between serine acetyltransferase and O-acetylserine (thiol) lyase in higher plants--structural and kinetic properties of the free and bound enzymes
    M Droux
    Laboratoire Mixte CNRS Rhône Poulenc UMR041, Rhône Poulenc Agrochimie, Lyon, France
    Eur J Biochem 255:235-45. 1998
  8. pmc O-phospho-L-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis
    Seán E O'Leary
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853 1301, USA
    Biochemistry 47:11606-15. 2008
  9. pmc Assembly of the cysteine synthase complex and the regulatory role of protein-protein interactions
    Sangaralingam Kumaran
    Donald Danforth Plant Science Center, St Louis, Missouri 63132, USA
    J Biol Chem 284:10268-75. 2009
  10. pmc Interaction of serine acetyltransferase with O-acetylserine sulfhydrylase active site: evidence from fluorescence spectroscopy
    Barbara Campanini
    Department of Biochemistry and Molecular Biology, Univeristy of Parma, 43100 Parma, Italy
    Protein Sci 14:2115-24. 2005

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Scientific Experts

Detail Information

Publications156 found, 100 shown here

  1. pmc Characterization of a novel thermostable O-acetylserine sulfhydrylase from Aeropyrum pernix K1
    Koshiki Mino
    Special Division for Human Life Technology, National Institute of Advanced Industrial Science and Technology AIST, Kansai, Ikeda, Osaka 563 8577, Japan
    J Bacteriol 185:2277-84. 2003
    ..51 mM, respectively, and a rate constant of 0.7 s(-1). A. pernix OASS has a high activity in the L-cysteine desulfurization reaction, which produces sulfide and S-(2,3-hydroxy-4-thiobutyl)-L-cysteine from L-cysteine and dithiothreitol...
  2. pmc Structural and biochemical studies of serine acetyltransferase reveal why the parasite Entamoeba histolytica cannot form a cysteine synthase complex
    Sudhir Kumar
    School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
    J Biol Chem 286:12533-41. 2011
    ..of EhSAT1 to form a hexamer and differences in the C-terminal region are likely to be the major reasons for the lack of formation of the large cysteine synthase complex and loss of a complex regulatory mechanism in E. histolytica.
  3. pmc Identification and functional analysis of Escherichia coli cysteine desulfhydrases
    Naoki Awano
    Department of Bioscience, Fukui Prefectural University, 4 1 1 Kenjojima, Matsuoka cho, Fukui 910 1195, Japan
    Appl Environ Microbiol 71:4149-52. 2005
    ..The gene disruption for each protein was significantly effective for overproduction of L-cysteine and L-cystine. Growth phenotype and transcriptional analyses suggest that tryptophanase contributes primarily to L-cysteine degradation...
  4. pmc A two-step process controls the formation of the bienzyme cysteine synthase complex
    Enea Salsi
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Parma, 43100 Parma, Italy
    J Biol Chem 285:12813-22. 2010
    ..One of the first regulatory complexes to be discovered was cysteine synthase (CS), formed by the pyridoxal 5'-phosphate-dependent enzyme O-acetylserine sulfhydrylase (OASS) and serine ..
  5. ncbi Use of biomolecular interaction analysis to elucidate the regulatory mechanism of the cysteine synthase complex from Arabidopsis thaliana
    Oliver Berkowitz
    Institute for Plant Genetics and Crop Plant Research IPK, Dept Molecular Cell Biology, Corrensstrasse 3, 06466 Gatersleben, Germany
    J Biol Chem 277:30629-34. 2002
    ..The association of the two enzyme subunits in the hetero-oligomeric cysteine synthase complex was investigated with respect to the reaction intermediate and putative effector O-acetylserine...
  6. doi Immunological responses to recombinant cysteine synthase A of Brucella abortus in BALB/c mice
    Shikha Jain
    Division of Microbiology, Defence Research and Development Establishment, Jhansi Road, Gwalior, 474 002 MP, India
    World J Microbiol Biotechnol 29:907-13. 2013
    ..In the present study, we generated recombinant cysteine synthase A (rCysK) of Brucella abortus in Escherichia coli and purified it up to homogeneity by metal affinity ..
  7. ncbi Interactions between serine acetyltransferase and O-acetylserine (thiol) lyase in higher plants--structural and kinetic properties of the free and bound enzymes
    M Droux
    Laboratoire Mixte CNRS Rhône Poulenc UMR041, Rhône Poulenc Agrochimie, Lyon, France
    Eur J Biochem 255:235-45. 1998
    ..O-acetylserine (thiol) lyase to form a bienzyme complex (molecular mass approximately 300 kDa) called cysteine synthase containing 4 mol pyridoxal 5'-phosphate/mol complex...
  8. pmc O-phospho-L-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis
    Seán E O'Leary
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853 1301, USA
    Biochemistry 47:11606-15. 2008
    The kinetic pathway of CysM, a cysteine synthase from Mycobacterium tuberculosis, was studied by transient-state kinetic techniques. The expression of which is upregulated under conditions of oxidative stress...
  9. pmc Assembly of the cysteine synthase complex and the regulatory role of protein-protein interactions
    Sangaralingam Kumaran
    Donald Danforth Plant Science Center, St Louis, Missouri 63132, USA
    J Biol Chem 284:10268-75. 2009
    Macromolecular assemblies play critical roles in regulating cellular functions. The cysteine synthase complex (CSC), which is formed by association of serine O-acetyltransferase (SAT) and O-acetylserine sulfhydrylase (OASS), acts as a ..
  10. pmc Interaction of serine acetyltransferase with O-acetylserine sulfhydrylase active site: evidence from fluorescence spectroscopy
    Barbara Campanini
    Department of Biochemistry and Molecular Biology, Univeristy of Parma, 43100 Parma, Italy
    Protein Sci 14:2115-24. 2005
    ....
  11. pmc Cysteine synthase overexpression in tobacco confers tolerance to sulfur-containing environmental pollutants
    M Noji
    Graduate School of Pharmaceutical Sciences, Department of Molecular Biology and Biotechnology, Chiba University, Yayoi-cho 1-33, Inage-ku, Chiba 263-8522, Japan
    Plant Physiol 126:973-80. 2001
    ..Furthermore, the leaves of F(1) plants exhibited the increased resistance to paraquat, a herbicide generating active oxygen species...
  12. doi Cysteine synthase (CysM) of Mycobacterium tuberculosis is an O-phosphoserine sulfhydrylase: evidence for an alternative cysteine biosynthesis pathway in mycobacteria
    Daniel Agren
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 283:31567-74. 2008
    ..We here show that the cysteine synthase CysM is, in contrast to previous annotations, an O-phosphoserine-specific cysteine synthase...
  13. pmc The active site of O-acetylserine sulfhydrylase is the anchor point for bienzyme complex formation with serine acetyltransferase
    Bin Huang
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Bacteriol 187:3201-5. 2005
    ..The aerobic form of OASS forms a tight bienzyme complex with SAT in vivo, termed cysteine synthase. We have determined the crystal structure of OASS in complex with a C-terminal peptide of SAT required for ..
  14. ncbi Molecular cloning and characterization of the genes encoding two isoforms of cysteine synthase in the enteric protozoan parasite Entamoeba histolytica
    T Nozaki
    Department of Tropical Medicine and Parasitology, Keio University, School of Medicine, Tokyo, Japan
    Mol Biochem Parasitol 97:33-44. 1998
    The enteric protozoan parasite Entamoeba histolytica was shown to possess cysteine synthase (CS) activity. The cDNA and genomic clones that encode two isoforms of the E...
  15. pmc Design of O-acetylserine sulfhydrylase inhibitors by mimicking nature
    Enea Salsi
    Department of Biochemistry and Molecular Biology, University of Parma, Italy
    J Med Chem 53:345-56. 2010
    ..These results, combined with a GRID analysis of the active site, allowed us to define a pharmacophoric scaffold for the design of peptidomimetic inhibitors...
  16. ncbi Molecular and biochemical analysis of the enzymes of cysteine biosynthesis in the plant Arabidopsis thaliana
    R Hell
    Department of Molecular Cell Biology, Institute for Plant Genetics and Crop Plant Research IPK, Leibniz Institute, Gatersleben, Germany
    Amino Acids 22:245-57. 2002
    ....
  17. doi A mechanistic model of the cysteine synthase complex
    Anna Feldman-Salit
    Molecular and Cell Modeling Group, EML Research, Schloss Wolfsbrunnenweg 33, 69118 Heidelberg, Germany anna feldman salit eml r villa bosch de
    J Mol Biol 386:37-59. 2009
    ..Cysteine biosynthesis involves a bienzyme complex, the cysteine synthase (CS) complex...
  18. ncbi Structure and mechanism of O-acetylserine sulfhydrylase
    Wael M Rabeh
    Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, USA
    J Biol Chem 279:26803-6. 2004
    ..The transition state for the elimination of acetic acid is thought to be asynchronous and earlier for Cbeta-O bond cleavage than for Calpha-H bond cleavage...
  19. ncbi Heavy metal tolerance of transgenic tobacco plants over-expressing cysteine synthase
    Cintia Goulart Kawashima
    Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University, Yayoi cho 1 33, Inage Ku, Chiba 263 8522, Japan
    Biotechnol Lett 26:153-7. 2004
    b>Cysteine synthase [O-acetyl-L-serine(thiol)lyase] catalyzes the final step for L-cysteine biosynthesis in plants...
  20. ncbi Structural insights into catalysis and inhibition of O-acetylserine sulfhydrylase from Mycobacterium tuberculosis. Crystal structures of the enzyme alpha-aminoacrylate intermediate and an enzyme-inhibitor complex
    Robert Schnell
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 282:23473-81. 2007
    ..The structure of the enzyme-peptide complex provides a framework for the design of strong binding inhibitors...
  21. pmc Structure and function of the hetero-oligomeric cysteine synthase complex in plants
    Markus Wirtz
    Heidelberg Institute for Plant Sciences, University of Heidelberg, Im Neuenheimer Feld 360, 69120 Heidelberg, Germany
    J Biol Chem 285:32810-7. 2010
    ..serine acetyltransferase (SAT) and O-acetylserine (thiol)-lyase (OAS-TL), which form the hetero-oligomeric cysteine synthase complex (CSC)...
  22. ncbi An age-induced switch to a hyper-recombinational state
    Michael A McMurray
    Division of Basic Sciences, The Fred Hutchinson Cancer Research Center, and Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98109, USA
    Science 301:1908-11. 2003
    ..In young cells, LOH occurs by reciprocal recombination, whereas LOH in old cells was nonreciprocal, occurring predominantly in the old mother's progeny. Thus, nuclear genomes may be inherently unstable with age...
  23. ncbi The cytosolic O-acetylserine(thiol)lyase gene is regulated by heavy metals and can function in cadmium tolerance
    J R Dominguez-Solís
    Instituto de Bioquimica Vegetal y Fotosintesis, Centro de Investigaciones Cientificas Isla de la Cartuja, Consejo Superior de Investigaciones Cientificas and Universidad de Sevilla, Avda Americo Vespucio s n, 41092 Sevilla, Spain
    J Biol Chem 276:9297-302. 2001
    ..Cadmium accumulates to higher levels in leaves of tolerant transformed lines than in wild-type plants...
  24. ncbi The cysteine synthase complex from plants. Mitochondrial serine acetyltransferase from Arabidopsis thaliana carries a bifunctional domain for catalysis and protein-protein interaction
    M Wirtz
    Institut fur Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany
    Eur J Biochem 268:686-93. 2001
    ..in bacteria and plants and functions in association with O-acetylserine (thiol) lyase (OAS-TL) in the cysteine synthase complex...
  25. pmc Role of a cysteine synthase in Staphylococcus aureus
    James K Lithgow
    Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    J Bacteriol 186:1579-90. 2004
    ..aureus cysM is sequentially and functionally homologous to the O-acetylserine (thiol)-lyase B family of cysteine synthase proteins. An S...
  26. ncbi Overproduction of SAT and/or OASTL in transgenic plants: a survey of effects
    Agnieszka Sirko
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul Pawinskiego 5a, 02 106 Warsaw, Poland
    J Exp Bot 55:1881-8. 2004
    ..of cysteine biosynthesis are catalysed by a bi-enzyme complex composed of serine acetyltransferase (SAT) and cysteine synthase, also called O-acetyl-serine (thiol) lyase (OASTL)...
  27. ncbi Molecular basis of cysteine biosynthesis in plants: structural and functional analysis of O-acetylserine sulfhydrylase from Arabidopsis thaliana
    Eric R Bonner
    Donald Danforth Plant Science Center, St Louis, Missouri 63132, USA
    J Biol Chem 280:38803-13. 2005
    ..Initial protein-protein interaction experiments using AtOASS mutants targeted to this loop support this hypothesis...
  28. ncbi Molecular and biochemical analysis of serine acetyltransferase and cysteine synthase towards sulfur metabolic engineering in plants
    M Noji
    Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University, Japan
    Amino Acids 22:231-43. 2002
    Serine acetyltransferase (SATase) and cysteine synthase (O-acetylserine (thiol)-lyase) (CSase) are committed in the final step of cysteine biosynthesis. Six cDNA clones encoding SATase have been isolated from several plants, e.g...
  29. ncbi Beta-cyanoalanine synthase and cysteine synthase from potato: molecular cloning, biochemical characterization, and spatial and hormonal regulation
    A Maruyama
    Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University, Japan
    Plant Mol Biol 46:749-60. 2001
    ..4.1.9) is the most important enzyme in cyanide metabolism. In addition to CAS, cysteine synthase (CS, EC 4.2.99.8) possesses CAS activity...
  30. pmc Limits to sulfur accumulation in transgenic lupin seeds expressing a foreign sulfur-rich protein
    Linda M Tabe
    Commonwealth Scientific and Industrial Research Organization, Plant Industry, Canberra, Australian Capital Territory, Australia
    Plant Physiol 128:1137-48. 2002
    ..Uptake of sulfur by lupin cotyledons, as indicated by total seed sulfur at maturity, responded positively to increased sulfur supply, but not to increased demand in the Tg seeds...
  31. ncbi Functional analysis of the cysteine synthase protein complex from plants: structural, biochemical and regulatory properties
    Markus Wirtz
    Heidelberg Institute of Plant Sciences HIP, University of Heidelberg, Im Neuenheimer Feld 360, 69120 Heidelberg, Germany
    J Plant Physiol 163:273-86. 2006
    ..Together they form the hetero-oligomeric cysteine synthase complex (CSC)...
  32. ncbi Genomic and functional characterization of the oas gene family encoding O-acetylserine (thiol) lyases, enzymes catalyzing the final step in cysteine biosynthesis in Arabidopsis thaliana
    R Jost
    Institute for Plant Genetics and Crop Plant Research IPK, Molecular Cell Biology Department, 06466, Gatersleben, Germany
    Gene 253:237-47. 2000
    ..that the mitochondrial OAS-TL C exhibits in vivo protein-protein interaction capabilities with respect to cysteine synthase complex formation similar to cytosolic OAS-TL A and plastid OAS-TL B...
  33. ncbi Cysteine synthesis in plants: protein-protein interactions of serine acetyltransferase from Arabidopsis thaliana
    N Bogdanova
    Lehrstuhl fur Pflanzenphysiologie, Ruhr Universitat Bochum, Germany
    Plant J 11:251-62. 1997
    ..sequential action of serine acetyltransferase (SAT) and O-acetylserine (thiol) lyase (OAS-TL) which form a cysteine synthase (CS) complex in vitro...
  34. ncbi Identification of aluminum-responsive proteins in rice roots by a proteomic approach: cysteine synthase as a key player in Al response
    Qiaosong Yang
    Laboratory of Molecular Plant Physiology, College of Life Sciences, South China Agricultural University, Guangzhou, China
    Proteomics 7:737-49. 2007
    ..More importantly, a number of other identified proteins including cysteine synthase (CS), 1-aminocyclopropane-1-carboxylate oxidase, G protein beta subunit-like protein, abscisic acid- and ..
  35. ncbi Functional analysis of the Bacillus subtilis cysK and cysJI genes
    J R van der Ploeg
    Institute of Microbiology, Swiss Federal Institute of Technology, ETH Zentrum, CH 8092 Zurich, Switzerland
    FEMS Microbiol Lett 201:29-35. 2001
    ..A strain in which cysK and yrhA, a cysK paralog, were inactivated was unable to grow with sulfate. Whereas expression of the cysJI genes was induced by sulfate, expression of cysK was repressed both by sulfate and by cysteine...
  36. pmc Dominant-negative modification reveals the regulatory function of the multimeric cysteine synthase protein complex in transgenic tobacco
    Markus Wirtz
    Heidelberg Institute of Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
    Plant Cell 19:625-39. 2007
    ..These data provide evidence that the CSC and its subcellular compartmentation play a crucial role in the control of Cys biosynthesis, a unique function for a plant metabolic protein complex...
  37. pmc Structural basis for interaction of O-acetylserine sulfhydrylase and serine acetyltransferase in the Arabidopsis cysteine synthase complex
    Julie A Francois
    Donald Danforth Plant Science Center, St Louis, Missouri 63132, USA
    Plant Cell 18:3647-55. 2006
    ..These results provide new insights into the molecular mechanism underlying formation of the Cys synthase complex and provide a structural basis for the biochemical regulation of Cys biosynthesis in plants...
  38. ncbi Molecular cloning and expression analyses of mitochondrial and plastidic isoforms of cysteine synthase (O-acetylserine(thiol)lyase) from Arabidopsis thaliana
    H Hesse
    Institut fur Genbiologische Forschung GmbH, Berlin, Federal Republic of Germany
    Amino Acids 16:113-31. 1999
    b>Cysteine synthase, the key enzyme for fixation of inorganic sulfide, catalyses the formation of cysteine from O-acetylserine and inorganic sulfide...
  39. pmc beta-Cyanoalanine synthase is a mitochondrial cysteine synthase-like protein in spinach and Arabidopsis
    Y Hatzfeld
    Chiba University, Faculty of Pharmaceutical Sciences, Laboratory of Molecular Biology and Biotechnology, Yayoi cho 1 33, Inage Ku, Chiba 263 8522, Japan
    Plant Physiol 123:1163-71. 2000
    ..We hypothesized that the mitochondrial cysteine synthase (CS; EC 4.2.99...
  40. ncbi Three Arabidopsis genes encoding proteins with differential activities for cysteine synthase and beta-cyanoalanine synthase
    Y Yamaguchi
    Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma, Japan
    Plant Cell Physiol 41:465-76. 2000
    ..thaliana genome. Escherichia coli complementation analyses confirmed that the cDNAs encode cysteine synthase and the corresponding proteins produced in E. coli clearly showed cysteine synthase activity...
  41. ncbi Three-dimensional structure of O-acetylserine sulfhydrylase from Salmonella typhimurium
    P Burkhard
    Department of Structural Biology, Biozentrum, University of Basel, Klingelbergstrasse70, Basel, CH 4056, Switzerland
    J Mol Biol 283:121-33. 1998
    ..The active site cleft of O-acetylserine sulfhydrylase is wider and therefore more exposed to the solvent. A possible binding site for the substrate O-acetylserine is discussed...
  42. ncbi Time-resolved fluorescence of O-acetylserine sulfhydrylase
    S Benci
    Institute of Physical Sciences, University of Parma, Italy
    Biochim Biophys Acta 1429:317-30. 1999
    ....
  43. pmc A genetic screen for increased loss of heterozygosity in Saccharomyces cerevisiae
    Marguerite P Andersen
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genetics 179:1179-95. 2008
    ..Altogether, this study defines a genetic landscape for the basis of increased LOH and the processes by which it occurs...
  44. ncbi Pyridoxal 5'-phosphate-dependent alpha,beta-elimination reactions: mechanism of O-acetylserine sulfhydrylase
    C H Tai
    Department of Chemical Engineering, Chenshiu Institute of Technology, Kaohsiung, Taiwan, Republic of China
    Acc Chem Res 34:49-59. 2001
    ..O-Acetylserine sulfhydrylase is the only known pyridoxal 5'-phosphate-dependent enzyme that catalyzes a beta-elimination reaction to have an anti E2 mechanism...
  45. ncbi Ligand binding induces a large conformational change in O-acetylserine sulfhydrylase from Salmonella typhimurium
    P Burkhard
    Biozentrum, University of Basel, Klingelbergstrasse 70, Basel, CH 4056, Switzerland
    J Mol Biol 291:941-53. 1999
    ....
  46. ncbi Characterization of two genes encoding putative cysteine synthase required for cysteine biosynthesis in Schizosaccharomyces pombe
    Yasuko Fujita
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Ikenobe, Japan
    Biosci Biotechnol Biochem 68:306-11. 2004
    b>Cysteine synthase catalyzes the formation of cysteine from O-acetylserine, and is the key enzyme for de novo cysteine biosynthesis in Schizosaccharomyces pombe. An examination of the S...
  47. pmc Engineering Escherichia coli for increased productivity of serine-rich proteins based on proteome profiling
    Mee Jung Han
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Republic of Korea
    Appl Environ Microbiol 69:5772-81. 2003
    ..we designed a strategy to enhance the leptin productivity by manipulating the cysK gene, encoding cysteine synthase A. By coexpression of the cysK gene, we were able to increase the cell growth rate by approximately twofold...
  48. pmc Cysteine biosynthesis in Trichomonas vaginalis involves cysteine synthase utilizing O-phosphoserine
    Gareth D Westrop
    Division of Infection and Immunity, Institute of Biomedical and Life Sciences and Wellcome Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G12 8TA, Scotland, United Kingdom
    J Biol Chem 281:25062-75. 2006
    ..We report here that for synthesis of cysteine from sulfide, T. vaginalis relies upon cysteine synthase. The enzyme (TvCS1) can use either O-acetylserine or O-phosphoserine as substrates...
  49. pmc Cysteine metabolism-related genes and bacterial resistance to potassium tellurite
    Derie E Fuentes
    Departamento de Biologia, Facultad de Quimica y Biologia, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile
    J Bacteriol 189:8953-60. 2007
    ..Results showed that cell tolerance to tellurite correlates with the expression level of the cysteine metabolic genes and that these genes are up-regulated when tellurite is present in the growth medium...
  50. doi Effect of mutation of lysine-120, located at the entry to the active site of O-acetylserine sulfhydrylase-A from Salmonella typhimurium
    Chia Hui Tai
    Department of Chemical and Materials Engineering, Chenshiu University, No 840 Chengcing Rd, Kaohsiung, Taiwan, 83347
    Biochim Biophys Acta 1784:629-37. 2008
    ..Data suggest a role of K120 in helping to stabilize the closed conformation by participating in a new hydrogen bond to the backbone carbonyl of A231...
  51. ncbi A novel O-phospho-L-serine sulfhydrylation reaction catalyzed by O-acetylserine sulfhydrylase from Aeropyrum pernix K1
    Koshiki Mino
    The Special Division for Human Life Technology, National Institute of Advanced Industrial Science and Technology, 1 8 31 Midorigaoka, Ikeda, 563 8577 Osaka, Japan
    FEBS Lett 551:133-8. 2003
    ..This is the first report of an enzyme that catalyzes the O-phospho-L-serine sulfhydrylation reaction...
  52. ncbi High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli
    Georg Zocher
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Freiburg im Breisgau, Germany
    FEBS J 274:5382-9. 2007
    ..Although the homodimer asymmetry was most probably caused by crystal packing, it indicates that the enzyme can adopt such a state in solution, which may be relevant for the catalytic reaction...
  53. ncbi Chromate differentially affects the expression of a high-affinity sulfate transporter and isoforms of components of the sulfate assimilatory pathway in Zea mays (L.)
    M Schiavon
    Department of Agricultural Biotechnologies, University of Padua, Agripolis, 35020 Legnaro Padua, Italy
    Plant Biol (Stuttg) 9:662-71. 2007
    ..This work shows for the first time that chromate specifically represses sulfate uptake, and such repression occurs without the implication of the candidate regulatory metabolites of the sulfate transport system in plants...
  54. ncbi Structure, mechanism, and conformational dynamics of O-acetylserine sulfhydrylase from Salmonella typhimurium: comparison of A and B isozymes
    Arundhati Chattopadhyay
    Institute of Materials Science, University of Connecticut, 97 North Eagleville Road, Storrs, Connecticut 06269 3136, USA
    Biochemistry 46:8315-30. 2007
    ..This finding, in agreement with structural data, suggests for the O-acetylserine sulfhydrylase B-isozyme a higher degree of conformational flexibility linked to catalysis...
  55. ncbi Multiple fungal enzymes possess cysteine synthase activity in vitro
    Jerzy Brzywczy
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 5A Pawinskiego Street, 02 106 Warszawa, Poland
    Res Microbiol 158:428-36. 2007
    ..This activity is shared by cysteine synthase (CS) encoded by the cysB gene, homocysteine synthase encoded by cysD and by at least one more enzyme...
  56. ncbi Induction of cysteine dioxygenase activity by oral administration of cysteine analogues to the rat: implications for drug efficacy and safety
    E S Roopnarinesingh
    Section of Biological Chemistry, Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, South Kensington, UK
    Drug Metabol Drug Interact 21:75-86. 2005
    ....
  57. ncbi On the interaction site of serine acetyltransferase in the cysteine synthase complex from Escherichia coli
    Chunhui Zhao
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3 1 1 Tsushima naka, Okayama 700 8530, Japan
    Biochem Biophys Res Commun 341:911-6. 2006
    b>Cysteine synthase from Escherichia coli is a bienzyme complex comprised of serine acetyltransferase (SAT) and O-acetylserine sulfhydrylase A...
  58. ncbi Biochemical characterization of a thermostable cysteine synthase from Geobacillus stearothermophilus V
    C P Saavedra
    Laboratorio de Microbiologia Molecular, Facultad de Quimica y Biologia, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile
    Biochimie 86:481-5. 2004
    The cysK gene encoding a cysteine synthase of Geobacillus stearothermophilus V was overexpressed in E. coli and the recombinant protein was purified and characterized...
  59. ncbi Structure of the O-acetylserine sulfhydrylase isoenzyme CysM from Escherichia coli
    Michael T Claus
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Albertstrasse 21, D 79104 Freiburg im Breisgau, Germany
    Biochemistry 44:8620-6. 2005
    ..Since the structure of CysM revealed a remarkable main chain variation at the active center, it constitutes a further starting point for engineering mutants with novel substrate specificities...
  60. pmc Bacillus subtilis cysteine synthetase is a global regulator of the expression of genes involved in sulfur assimilation
    Daniela Albanesi
    Instituto de Biología Molecular y Celular de Rosario IBR CONICET and Departamento de Microbiología, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Argentina
    J Bacteriol 187:7631-8. 2005
    ..These results indicate that CysK, besides its enzymatic role in cysteine biosynthesis, is a global negative regulator of genes involved in sulfur metabolism...
  61. ncbi Crystal structure of O-acetylserine sulfhydrylase (TM0665) from Thermotoga maritima at 1.8 A resolution
    Andreas Heine
    The Joint Center for Structural Genomics, Stanford University, Menlo Park, California, USA
    Proteins 56:387-91. 2004
  62. ncbi [Sulfur-containing amino acid metabolism in parasitic protozoa]
    Tomoyoshi Nozaki
    Tanpakushitsu Kakusan Koso 47:21-9. 2002
  63. ncbi Studies on the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase in escherichia coli crude extract. Isolation of O-acetylserine sulfhydrylases A and B and beta-cystathionase based on their ability to mobilize sulfur from cysteine and to particip
    D H Flint
    E I Du Pont de Nemours and Company, Central Research and Development, Experimental Station, Wilmington, Delaware 19880 0328, USA
    J Biol Chem 271:16053-67. 1996
    ..coli where these factors are present, the rapidity with which Fe-S clusters are formed and the efficiency with which sulfur is used imply an orderly controlled formation of Fe-S clusters that is generally typified by enzymatic reactions...
  64. ncbi Effects of bienzyme complex formation of cysteine synthetase from escherichia coli on some properties and kinetics
    K Mino
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Japan
    Biosci Biotechnol Biochem 64:1628-40. 2000
    ....
  65. ncbi Two enzymes together capable of cysteine biosynthesis are encoded on a cyanobacterial plasmid
    M L Nicholson
    University of Western Ontario, Department of Plant Sciences, London, Canada
    Mol Gen Genet 247:623-32. 1995
    ..The possible function of these genes in the adaptation of cyanobacteria to sulfur stress is discussed...
  66. ncbi Unfolding of pyridoxal 5'-phosphate-dependent O-acetylserine sulfhydrylase probed by time-resolved tryptophan fluorescence
    Stefano Bettati
    Institute of Physical Sciences, University of Parma, Via Volturno 39, 43100 Parma, Italy
    Biochim Biophys Acta 1596:47-54. 2002
    ....
  67. pmc Occurrence of transsulfuration in synthesis of L-homocysteine in an extremely thermophilic bacterium, Thermus thermophilus HB8
    S Yamagata
    Department of Biotechnology, Faculty of Agriculture, Gifu University, Gifu 501-1193, Japan
    J Bacteriol 183:2086-92. 2001
    ..of O-acetylserine sulfhydrylase by 55 and 73%, respectively, confirming that this enzyme functions as a cysteine synthase. Methionine employed at 1 to 5 mM in the same way derepressed the synthesis of O-acetylserine sulfhydrylase ..
  68. ncbi Cysteine synthase of an extremely thermophilic bacterium, Thermus thermophilus HB8
    Yasuko Mizuno
    United Graduate School of Agricultural Sciences, Faculty of Agriculture, Gifu University, Japan
    Biosci Biotechnol Biochem 66:549-57. 2002
    ..The apo-enzyme showed an apparent Km of 29 microM for the cofactor at pH 8. Monoiodoacetic acid (1 mM) almost completely inactivated the enzyme. The meaning of a very high enzyme content in the cell is discussed...
  69. ncbi Biosynthesis of sulfur-containing amino acids in Streptomyces venezuelae ISP5230: roles for cystathionine beta-synthase and transsulfuration
    Z Chang
    Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4J1
    Microbiology 148:2135-47. 2002
    ..venezuelae ISP5230 genomic DNA was amplified by PCR using primers based on consensus sequences of cysteine synthase isozyme A from bacteria...
  70. ncbi Detection of intermediates in reactions catalyzed by PLP-dependent enzymes: O-acetylserine sulfhydrylase and serine-glyoxalate aminotransferase
    William E Karsten
    Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, USA
    Methods Enzymol 354:223-37. 2002
  71. ncbi Phylogeny of metabolic pathways: O-acetylserine sulphydrylase A is homologous to the tryptophan synthase beta subunit
    S Levy
    Unité de Régulation de l Expression Génétique, Institut Pasteur, Paris, France
    Mol Microbiol 2:777-83. 1988
    ..This may indicate that these proteins, although catalysing different reactions in different metabolic pathways, have evolved from a common ancestral gene...
  72. ncbi Characterization of the allosteric anion-binding site of O-acetylserine sulfhydrylase
    C H Tai
    Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, Oklahoma 73019, USA
    Biochemistry 40:7446-52. 2001
    ..Inhibition by sulfide is potent (K(d) is 25 microM at pH 8) suggesting that SH(-) is the physiologic inhibitory species...
  73. pmc Cysteine biosynthesis pathway in the archaeon Methanosarcina barkeri encoded by acquired bacterial genes?
    M Kitabatake
    Department of Molecular Biophysics and Biochemistry, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8114, USA
    J Bacteriol 182:143-5. 2000
    ....
  74. ncbi Characterization of the S272A,D site-directed mutations of O-acetylserine sulfhydrylase: involvement of the pyridine ring in the alpha,beta-elimination reaction
    Sebastian Daum
    Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, Oklahoma 73072, USA
    Biochemistry 42:106-13. 2003
    ..D., Jr., and Cook, P. F. (1994) Biochemistry 33, 1647-1683) has an intensity near zero, as compared to significant fluorescence for the wild-type enzyme...
  75. ncbi Molecular cloning of the cys genes (cysC, cysD, cysH, cysI, cysJ, and cysG) responsible for cysteine biosynthesis in Escherichia coli K-12
    H Tei
    Research Institute for Food Science, Kyoto University, Japan
    Biotechnol Appl Biochem 12:212-6. 1990
    ..The following results were obtained. First, the cysCDHIJ regulon could be separated into cysCD and cysHIJ regions. Second, the cysG gene was quite different from the cysI and cysJ genes...
  76. ncbi Purification and characterization of serine acetyltransferase from Escherichia coli partially truncated at the C-terminal region
    K Mino
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Japan
    Biosci Biotechnol Biochem 63:168-79. 1999
    ..However, SAT delta C20 did not form a complex with O-acetylserine sulfhydrylase-A (OASS-A), a counterpart of the cysteine synthetase and did not reduce OASS activity in contrast to the wild-type SAT and Met-256-Ile mutant SAT...
  77. ncbi Purification and characterization of O-acetylserine (thiol) lyase from spinach chloroplasts
    M Droux
    UM 41 CNRS, Rhône Poulenc Agrochimie, Centre de la Recherche de la Dargoire, Lyon, France
    Arch Biochem Biophys 295:379-90. 1992
    ..serine acetyltransferase at a position corresponding to a molecular mass of 310,000 (such a complex called cysteine synthase has been characterized in bacteria). The activity of O-acetylserine (thiol) lyase was optimum between pH 7...
  78. ncbi Identification of an allosteric anion-binding site on O-acetylserine sulfhydrylase: structure of the enzyme with chloride bound
    P Burkhard
    M E Müller Institute for Structural Biology
    J Mol Biol 303:279-86. 2000
    ..Chloride may be an analog of sulfide, the physiological inhibitor. Finally, these results suggest that OASS represents a new class of PLP-dependent enzymes that is regulated by small anions...
  79. ncbi Increase in the stability of serine acetyltransferase from Escherichia coli against cold inactivation and proteolysis by forming a bienzyme complex
    K Mino
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Japan
    Biosci Biotechnol Biochem 65:865-74. 2001
    ....
  80. ncbi Anti-stress activity of Propionibacterium freudenreichii : identification of a reactivative protein
    Lena Vorobjeva
    Department of Microbiology, Moscow State University, 119899 Moscow, Russia
    Antonie Van Leeuwenhoek 85:53-62. 2004
    ..The isolated 35 kDa protein was then identified using both N-terminal and internal peptide sequencing as a cysteine synthase. The latter was localised in the P. freudenreichii proteomic map...
  81. pmc Coproporphyrin excretion and low thiol levels caused by point mutation in the Rhodobacter sphaeroides S-adenosylmethionine synthetase gene
    Monique Sabaty
    Laboratoire de Bioenergetique Cellulaire, CEA Cadarache, DSV, IBEB, Saint Paul lez Durance, F 13108, France
    J Bacteriol 192:1238-48. 2010
    ..phototrophic conditions but has low levels of intracellular cysteine and glutathione and overexpresses the cysteine synthase CysK...
  82. ncbi High D-glucose-induced changes in endothelial Ca2+/EDRF signaling are due to generation of superoxide anions
    W F Graier
    Department of Medical Biochemistry, University of Graz, Austria
    Diabetes 45:1386-95. 1996
    ..This overshoot of O2- enhances agonist-stimulated Ca2+/EDRF signaling via a yet unknown mechanism...
  83. pmc The mercaptopyruvate sulfurtransferase of Trichomonas vaginalis links cysteine catabolism to the production of thioredoxin persulfide
    Gareth D Westrop
    Strathclyde Institute of Pharmacy and Biomedical Sciences, Univesity of Strathclyde, Glasgow G4 0NR, United Kingdom
    J Biol Chem 284:33485-94. 2009
    Trichomonas vaginalis is a protozoan parasite of humans that is able to synthesize cysteine de novo using cysteine synthase but does not produce glutathione...
  84. pmc Metabolome analysis revealed increase in S-methylcysteine and phosphatidylisopropanolamine synthesis upon L-cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica
    Afzal Husain
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Biol Chem 285:39160-70. 2010
    ..We further demonstrated that S-methylcysteine is synthesized from methanethiol and O-acetylserine by cysteine synthase, which was previously considered to be involved in sulfur-assimilatory L-cysteine biosynthesis...
  85. doi Kinetic characterization of methionine gamma-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis
    Dan Sato
    Department of Parasitology, Gunma University Graduate School of Medicine, Japan
    FEBS J 275:548-60. 2008
    ..It is unlikely that O-acetyl-l-serine is decomposed by EhMGLs, given the kinetic parameters of cysteine synthase reported previously...
  86. ncbi Characterization of the gene encoding serine acetyltransferase, a regulated enzyme of cysteine biosynthesis from the protist parasites Entamoeba histolytica and Entamoeba dispar. Regulation and possible function of the cysteine biosynthetic pathway in Ent
    T Nozaki
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Biol Chem 274:32445-52. 1999
    ..by L-cysteine in a competitive manner with L-serine; 2) inhibition by L-cystine; and 3) no association with cysteine synthase. Genetically engineered amoeba strains that overproduced cysteine synthase and SAT were created...
  87. ncbi Proteomic analyses of a robust versus a poor chicken gastrointestinal colonizing isolate of Campylobacter jejuni
    Bruce S Seal
    Poultry Microbiological Safety Research Unit, Russell Research Center, ARS, USDA, 950 College Station Road, Athens, Georgia 30605, USA
    J Proteome Res 6:4582-91. 2007
    ..Several proteins including a cysteine synthase and aconitate hydratase were detected specifically in the poor colonizer C. jejuni NCTC 11168-PMSRU isolate...
  88. ncbi Proteomic analysis of Brucella abortus cell envelope and identification of immunogenic candidate proteins for vaccine development
    Joseph P Connolly
    Vital Probes Inc, 1300 Old Plank Road, Mayfield, PA 18433, USA
    Proteomics 6:3767-80. 2006
    ..proteins such as fumarate reductase flavoprotein subunit, F0F1-type ATP synthase alpha subunit, and cysteine synthase A. The elucidation of the immunome of B...
  89. pmc Suppression of metallothionein-I/II expression and its probable molecular mechanisms
    Samson T Jacob
    Department of Molecular and Cellular Biochemistry, College of Medicine, The Ohio State University, 333 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210, USA
    Environ Health Perspect 110:827-30. 2002
    ..The expressions of GST-pi and gamma-glutamyl cysteine synthase were also significantly higher (5- to 12-fold) in the lymphosarcoma cells and the hepatoma relative to the ..
  90. ncbi Identification of novel VirR/VirS-regulated genes in Clostridium perfringens
    S Banu
    Department of Microbiology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    Mol Microbiol 35:854-64. 2000
    ..A 4.9 kb mRNA transcribing the metB (cystathionine gamma-synthase), cysK (cysteine synthase) and ygaG (hypothetical protein) genes was negatively regulated, whereas 1.6 and 6...
  91. pmc Molecular and functional analyses of the metC gene of Lactococcus lactis, encoding cystathionine beta-lyase
    M Fernandez
    Microbial Ingredients Section, NIZO Food Research, Ede, The Netherlands
    Appl Environ Microbiol 66:42-8. 2000
    ..The metC gene was found to be cotranscribed with a downstream cysK gene, which encodes a putative cysteine synthase. The MetC proteins of both strains were overproduced in strain MG1363 with the NICE (nisin-controlled ..
  92. ncbi Glutathione depletion and neuronal cell death: the role of reactive oxygen intermediates and mitochondrial function
    U Wullner
    Department of Neurology, Eberhard Karls University, Hoppe Seyler Str 3, D 72076, Tubingen, Germany
    Brain Res 826:53-62. 1999
    ..l-buthionine sulfoximine (BSO), which irreversibly blocks gamma-glutamyl-cysteine synthase, led to a time- and concentration-dependent loss of cytoplasmic GSH, while mitochondrial GSH was relatively ..
  93. pmc Stacking multiple transgenes at a selected genomic site via repeated recombinase-mediated DNA cassette exchanges
    Zhongsen Li
    DuPont Agricultural Biotechnology, Experimental Station E353, Wilmington, Delaware 19880, USA
    Plant Physiol 154:622-31. 2010
    ..synthetase gene (DHPS), a barley (Hordeum vulgare) high-lysine protein gene (BHL8), and a truncated soybean cysteine synthase gene (CGS), to improve the contents of the essential amino acids lysine and methionine...
  94. pmc Binding of cysteine synthase to the STAS domain of sulfate transporter and its regulatory consequences
    Nakako Shibagaki
    Department of Plant Biology, The Carnegie Institution for Science, Stanford, California 94305, USA
    J Biol Chem 285:25094-102. 2010
    ..The S(2-) is then bonded to O-acetylserine through the activity of cysteine synthase (O-acetylserine (thiol)lyase or OASTL) to form cysteine, the first organic molecule of the SO(4)(2-) ..
  95. doi Genomic differences between Campylobacter jejuni isolates identify surface membrane and flagellar function gene products potentially important for colonizing the chicken intestine
    Kelli L Hiett
    Agricultural Research Service, Poultry Microbiological Safety Research Unit, Russell Research Center, United States Department of Agriculture, P O Box 5677, Athens, GA, 30604 5677, USA
    Funct Integr Genomics 8:407-20. 2008
    ..Several proteins including cysteine synthase and a Ni/Fe hydrogenase were determined to be differentially present between the two isolates...
  96. ncbi cDNA cloning and expression of cysteine synthase B localized in chloroplasts of Spinacia oleracea
    K Saito
    Faculty of Pharmaceutical Sciences, Chiba University, Japan
    FEBS Lett 324:247-52. 1993
    The cDNA clones for cysteine synthase B, which is localized in chloroplasts of Spinacia oleracea L., were isolated by screening a library with synthetic oligonucleotides encoding a partial peptide sequence of the purified protein...
  97. ncbi Mitochondria are a primary target of hypericin phototoxicity: synergy of intracellular calcium mobilisation in cell killing
    Theodossis A Theodossiou
    Department of Medicine, The Rayne Institute, 5 University Street, University College London, London WC1E 6JJ, UK
    Int J Biochem Cell Biol 38:1946-56. 2006
    ..Depletion of intracellular glutathione (>85%) via inhibition of gamma-glutamyl-cysteine synthase had no effect on hypericin (5 microM) phototoxicity, thus precluding any direct oxidative involvement of ..
  98. pmc Entamoeba histolytica Phosphoserine aminotransferase (EhPSAT): insights into the structure-function relationship
    Vibhor Mishra
    Division of Molecular and Structural Biology, Central Drug Research Institute, Chattar Manzil Palace, Council of Scientific and Industrial Research CSIR, Lucknow 226001, India
    BMC Res Notes 3:52. 2010
    ..Besides this, phoshoserine produced by the enzyme can also be taken up directly by cysteine synthase as a substrate...
  99. ncbi Isolation of Nicotiana plumbaginifolia cDNAs encoding isoforms of serine acetyltransferase and O-acetylserine (thiol) lyase in a yeast two-hybrid system with Escherichia coli cysE and cysK genes as baits
    Frantz Liszewska
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
    Acta Biochim Pol 52:117-28. 2005
    ..serine acetyltransferase (SAT, the product of cysE gene) and O-acetylserine (thiol)lyase A, also termed cysteine synthase (OASTL-A, the product of cysK gene)...
  100. ncbi The Aspergillus nidulans metR gene encodes a bZIP protein which activates transcription of sulphur metabolism genes
    Renata Natorff
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 5A Pawińskiego Str, 02 106 Warszawa, Poland
    Mol Microbiol 49:1081-94. 2003
    ..Transcription of genes encoding sulphate permease, homocysteine synthase, cysteine synthase, ATP-sulphurylase, and sulphur controller--sconB is strongly regulated by the metR gene product and depends ..
  101. doi Lead bioaccumulation potential of an aquatic macrophyte Najas indica are related to antioxidant system
    Ragini Singh
    National Botanical Research Institute Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226 001, Uttar Pradesh, India
    Bioresour Technol 101:3025-32. 2010
    ..Further, Pb exposed plants showed significantly increased cysteine synthase and glutathione-S-transferase activity...

Research Grants28

  1. Structure and Function of Hexapeptide Acyltransferases
    STEVEN RODERICK; Fiscal Year: 2007
    ..and reaction mechanism of STA will be studied as a resolved enzyme and as a component of the binary cysteine synthase complex. STA is inhibited by cysteine, but must also utilize serine...
  2. Alcohol-induced epigenetic changes in the liver genome
    Samson Jacob; Fiscal Year: 2007
    ..This proposal also fits well with the mission of the National Institute of Alcohol Abuse and Alcoholism on "alcohol metabolism and epigenetic effects on tissue injury". ..
  3. MOLECULAR MECHANISMS OF DIET-INDUCED CARCINOGENESIS
    Samson Jacob; Fiscal Year: 2006
    ..abstract_text> ..
  4. Role of metallothioneins in hepatocellular carcinoma
    Samson Jacob; Fiscal Year: 2007
    ..This proposal also fits well with the mission/goals of multiple institutes (NCI, NIDK, NIAA) and is consistent with the program announcement on etiology, prevention and treatment of hepatocellular carcinomas. ..
  5. Lysine Biosynthesis in Yeast
    Paul Cook; Fiscal Year: 2007
    ..Additionally, possible catalytic and binding residues in the homocitrate synthase have been identified by multiple sequence alignment, and alanine scanning mutagenesis will be applied to these. ..
  6. MOLECULAR MECHANISMS OF METALLOTHIONEIN INDUCTION
    Samson Jacob; Fiscal Year: 2003
    ..Because MT induction is repressed in many cancer cells due to hypermethylation, the re-expresssion of MT may be one effective means to arrest malignant growth of these cells. ..
  7. DNA METHYLATION AND GENE EXPRESSION IN CANCER CELLS
    Samson Jacob; Fiscal Year: 2004
    ..It is hoped that this study could provide the impetus to explore ways to reactivate these repressed genes that may result in the arrest of specific neoplastic growth. ..
  8. Molecular mechanism of diet induced carcinogenesis
    Samson Jacob; Fiscal Year: 2009
    ..This proposal also fits well with the recent program announcement of multiple institutes on diet, epigenetic events and cancer prevention. ..