transferases

Summary

Summary: Transferases are enzymes transferring a group, for example, the methyl group or a glycosyl group, from one compound (generally regarded as donor) to another compound (generally regarded as acceptor). The classification is based on the scheme "donor:acceptor group transferase". (Enzyme Nomenclature, 1992) EC 2.

Top Publications

  1. ncbi 3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo) transferase (WaaA) and kdo kinase (KdkA) of Haemophilus influenzae are both required to complement a waaA knockout mutation of Escherichia coli
    W Brabetz
    Division of Medical and Biochemical Microbiology, Research Center Borstel, Center for Medicine and Biosciences, Parkallee 22, D 23845 Borstel, Germany
    J Biol Chem 275:34954-62. 2000
  2. ncbi A new enzyme superfamily - the phosphopantetheinyl transferases
    R H Lambalot
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Chem Biol 3:923-36. 1996
  3. doi The herbicide ketoclomazone inhibits 1-deoxy-D-xylulose 5-phosphate synthase in the 2-C-methyl-D-erythritol 4-phosphate pathway and shows antibacterial activity against Haemophilus influenzae
    Yukiko Matsue
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, Japan
    J Antibiot (Tokyo) 63:583-8. 2010
  4. ncbi Cloning and characterization of the dxs gene, encoding 1-deoxy-d-xylulose 5-phosphate synthase from Agrobacterium tumefaciens, and its overexpression in Agrobacterium tumefaciens
    Jung Kul Lee
    Department of Chemical Engineering, Konkuk University, 1 Hwayang dong, Gwangjin gu, Seoul 143 701, Republic of Korea
    J Biotechnol 128:555-66. 2007
  5. ncbi Rhodobacter capsulatus 1-deoxy-D-xylulose 5-phosphate synthase: steady-state kinetics and substrate binding
    Lisa M Eubanks
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 42:1140-9. 2003
  6. doi Chapter 10 using phosphopantetheinyl transferases for enzyme posttranslational activation, site specific protein labeling and identification of natural product biosynthetic gene clusters from bacterial genomes
    Murat Sunbul
    Department of Chemistry, The University of Chicago, Chicago, Illinois, USA
    Methods Enzymol 458:255-75. 2009
  7. doi Functional characterization of the three genes encoding 1-deoxy-D-xylulose 5-phosphate synthase in maize
    Elizabeth Cordoba
    Departamento de Biologia Molecular de Plantas, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Av Universidad 2001 Chamilpa Apdo Postal 510 3 Cuernavaca, Morelos 62210, Mexico
    J Exp Bot 62:2023-38. 2011
  8. pmc Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol
    G A Sprenger
    Institut für Biotechnologie 1 des Forschungszentrums Jülich, Germany
    Proc Natl Acad Sci U S A 94:12857-62. 1997
  9. pmc Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis
    L M Lois
    Departament de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, Marti i Franques 1, 08028 Barcelona, Spain
    Proc Natl Acad Sci U S A 95:2105-10. 1998
  10. pmc Inactivation of Lgt allows systematic characterization of lipoproteins from Listeria monocytogenes
    Maja Baumgärtner
    Department of Cell Biology, Helmholtz Centre for Infection Research HZI, D 38124 Braunschweig, Germany
    J Bacteriol 189:313-24. 2007

Detail Information

Publications270 found, 100 shown here

  1. ncbi 3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo) transferase (WaaA) and kdo kinase (KdkA) of Haemophilus influenzae are both required to complement a waaA knockout mutation of Escherichia coli
    W Brabetz
    Division of Medical and Biochemical Microbiology, Research Center Borstel, Center for Medicine and Biosciences, Parkallee 22, D 23845 Borstel, Germany
    J Biol Chem 275:34954-62. 2000
    ..The structure was determined by nuclear magnetic resonance spectroscopy of deacylated LPS. Therefore, the reaction products of both cloned Kdo kinases represent only one of the two chemical structures synthesized by H. influenzae I69...
  2. ncbi A new enzyme superfamily - the phosphopantetheinyl transferases
    R H Lambalot
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Chem Biol 3:923-36. 1996
    ..Surprisingly, initial searches of sequence databases did not reveal any proteins with significant peptide sequence similarity with ACPS...
  3. doi The herbicide ketoclomazone inhibits 1-deoxy-D-xylulose 5-phosphate synthase in the 2-C-methyl-D-erythritol 4-phosphate pathway and shows antibacterial activity against Haemophilus influenzae
    Yukiko Matsue
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, Japan
    J Antibiot (Tokyo) 63:583-8. 2010
    ..These data reveal the inhibitory mechanism of ketoclomazone on DXP synthase...
  4. ncbi Cloning and characterization of the dxs gene, encoding 1-deoxy-d-xylulose 5-phosphate synthase from Agrobacterium tumefaciens, and its overexpression in Agrobacterium tumefaciens
    Jung Kul Lee
    Department of Chemical Engineering, Konkuk University, 1 Hwayang dong, Gwangjin gu, Seoul 143 701, Republic of Korea
    J Biotechnol 128:555-66. 2007
    ..9 and 23.9%, respectively. This work describes Dxs from A. tumefaciens, an organism with the potential for industrial UbiQ(10) production, and the first metabolic engineering study with the non-mevalonate pathway enzyme in A. tumefaciens...
  5. ncbi Rhodobacter capsulatus 1-deoxy-D-xylulose 5-phosphate synthase: steady-state kinetics and substrate binding
    Lisa M Eubanks
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 42:1140-9. 2003
    ..These results are consistent with an ordered mechanism for DXP synthase where pyruvate binds before GAP/d-glyceraldehyde...
  6. doi Chapter 10 using phosphopantetheinyl transferases for enzyme posttranslational activation, site specific protein labeling and identification of natural product biosynthetic gene clusters from bacterial genomes
    Murat Sunbul
    Department of Chemistry, The University of Chicago, Chicago, Illinois, USA
    Methods Enzymol 458:255-75. 2009
    Phosphopantetheinyl transferases (PPTases) covalently attach the phosphopantetheinyl group derived from coenzyme A (CoA) to acyl carrier proteins or peptidyl carrier proteins as part of the enzymatic assembly lines of fatty acid synthases ..
  7. doi Functional characterization of the three genes encoding 1-deoxy-D-xylulose 5-phosphate synthase in maize
    Elizabeth Cordoba
    Departamento de Biologia Molecular de Plantas, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Av Universidad 2001 Chamilpa Apdo Postal 510 3 Cuernavaca, Morelos 62210, Mexico
    J Exp Bot 62:2023-38. 2011
    ..mays, DXS protein is feedback regulated in response to the inhibition of the pathway flow. The results support that the multilevel regulation of DXS activity is conserved in evolution...
  8. pmc Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol
    G A Sprenger
    Institut für Biotechnologie 1 des Forschungszentrums Jülich, Germany
    Proc Natl Acad Sci U S A 94:12857-62. 1997
    ....
  9. pmc Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis
    L M Lois
    Departament de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, Marti i Franques 1, 08028 Barcelona, Spain
    Proc Natl Acad Sci U S A 95:2105-10. 1998
    ..D-1-Deoxyxylulose-5-phosphate synthase belongs to a family of transketolase-like proteins that are highly conserved in evolution...
  10. pmc Inactivation of Lgt allows systematic characterization of lipoproteins from Listeria monocytogenes
    Maja Baumgärtner
    Department of Cell Biology, Helmholtz Centre for Infection Research HZI, D 38124 Braunschweig, Germany
    J Bacteriol 189:313-24. 2007
    ..It is noteworthy that in contrast to previous studies of Escherichia coli, we unambiguously demonstrated that lipidation by Lgt is not a prerequisite for activity of the lipoprotein-specific signal peptidase II (Lsp) in Listeria...
  11. ncbi Properties and inhibition of the first two enzymes of the non-mevalonate pathway of isoprenoid biosynthesis
    C Mueller
    Botanical Institute II, University of Karlsruhe, Kaiserstr 12, D 76128 Karlsruhe, Germany
    Biochem Soc Trans 28:792-3. 2000
    ..The DXR of plants, isolated from barley seedlings, shows a pH optimum of 8.1, which is typical for enzymes active in the chloroplast stroma...
  12. pmc KpsC and KpsS are retaining 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) transferases involved in synthesis of bacterial capsules
    Lisa M Willis
    Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada N1G 2W1
    Proc Natl Acad Sci U S A 110:20753-8. 2013
    ..To date, the only characterized Kdo transferases are the inverting enzymes that catalyze the α-linkages found in lipopolysaccharide...
  13. pmc The essential OST2 gene encodes the 16-kD subunit of the yeast oligosaccharyltransferase, a highly conserved protein expressed in diverse eukaryotic organisms
    S Silberstein
    Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01655 0103, USA
    J Cell Biol 131:371-83. 1995
    ..The protein sequence of ost2 mutant alleles revealed mutations at highly conserved residues in the Ost2p/DAD1 protein sequence...
  14. pmc Lgt processing is an essential step in Streptococcus suis lipoprotein mediated innate immune activation
    Paul J Wichgers Schreur
    Central Veterinary Institute, Wageningen UR, Lelystad, The Netherlands
    PLoS ONE 6:e22299. 2011
    ..suis able to activate the innate immune system likely include cell wall constituents that may be released during growth or after cell wall integrity loss, however characterization of these components is still limited...
  15. ncbi Two distantly related genes encoding 1-deoxy-d-xylulose 5-phosphate synthases: differential regulation in shoots and apocarotenoid-accumulating mycorrhizal roots
    Michael H Walter
    Leibniz Institut für Pflanzenbiochemie, Abteilung Sekundärstoffwechsel, Weinberg 3, D 06120 Halle Saale, Germany
    Plant J 31:243-54. 2002
    ..The potential importance of DXS1 in many housekeeping functions and a still hypothetical role of DXS2 in the biosynthesis of secondary isoprenoids is discussed...
  16. ncbi Tools for discovery of inhibitors of the 1-deoxy-D-xylulose 5-phosphate (DXP) synthase and DXP reductoisomerase: an approach with enzymes from the pathogenic bacterium Pseudomonas aeruginosa
    B Altincicek
    Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, Giessen, Germany
    FEMS Microbiol Lett 190:329-33. 2000
    ..A novel and convenient spectrophotometric assay was developed to determine activity and inhibition of P. aeruginosa DXP synthase. Fluoropyruvate is described as a first inhibitor of DXP synthase...
  17. pmc Structure-activity relationships of compounds targeting mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate synthase
    Jialin Mao
    Drug Discovery Program, Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA
    Bioorg Med Chem Lett 18:5320-3. 2008
    ..Ultimately we found that 2-methyl-3-(4-fluorophenyl)-5-(4-methoxy-phenyl)-4H-pyrazolo[1,5-a]pyrimidin-7-one, although still weak, was able to inhibit M. tuberculosis DXS with an IC(50) of 10.6 microM...
  18. ncbi Topological analysis of the MraY protein catalysing the first membrane step of peptidoglycan synthesis
    A Bouhss
    Biochimie Structurale et Cellulaire, CNRS, Batiment 430, Universite Paris Sud, F 91405, Orsay, France
    Mol Microbiol 34:576-85. 1999
    The two-dimensional membrane topology of the Escherichia coli and Staphylococcus aureus MraY transferases, which catalyse the formation of the first lipid intermediate of peptidoglycan synthesis, was established using the beta-lactamase ..
  19. pmc Frequency of conjugative transfer of plasmid-encoded ISEcp1 - blaCTX-M-15 and aac(6')-lb-cr genes in Enterobacteriaceae at a tertiary care center in Lebanon - role of transferases
    Mohamad Harajly
    Department of Microbiology and Immunology, American University of Beirut, Lebanon
    Ann Clin Microbiol Antimicrob 9:19. 2010
    ..In addition, the role of tra genes encoding transferases in mediating conjugation was assessed.
  20. ncbi The oligosaccharyltransferase complex from yeast
    R Knauer
    Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universitat Regensburg, 93040 Regensburg, Germany
    Biochim Biophys Acta 1426:259-73. 1999
    ....
  21. ncbi Lipid modification of prelipoproteins is dispensable for growth but essential for efficient protein secretion in Bacillus subtilis: characterization of the Lgt gene
    S Leskela
    Laboratory of Vaccine Development, National Public Health Institute, Helsinki, Finland
    Mol Microbiol 31:1075-85. 1999
    ..There was no indication that non-lipomodified PrsA displayed reduced activity...
  22. pmc DXP synthase-catalyzed C-N bond formation: nitroso substrate specificity studies guide selective inhibitor design
    Francine Morris
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, 725 North Wolfe St, Baltimore, MD 21205, USA
    Chembiochem 14:1309-15. 2013
    ..As a proof of concept, we show selective inhibition of DXP synthase by benzylacetylphosphonate (BnAP)...
  23. doi Transferases and transporters mediate the detoxification and capacity to tolerate trinitrotoluene in Arabidopsis
    Premysl Landa
    Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany AS CR, vvi and Research Institute of Crop Production, vvi, Prague, Czech Republic
    Funct Integr Genomics 10:547-59. 2010
    ....
  24. pmc Mutations in Escherichia coli aceE and ribB genes allow survival of strains defective in the first step of the isoprenoid biosynthesis pathway
    Jordi Perez-Gil
    Centre for Research in Agricultural Genomics CRAG CSIC IRTA UAB UB, Campus UAB Bellaterra, Barcelona, Spain
    PLoS ONE 7:e43775. 2012
    ..Together, this work unveils the diversity of mechanisms that can evolve in bacteria to circumvent a blockage of the first step of the MEP pathway...
  25. pmc Revealing substrate promiscuity of 1-deoxy-D-xylulose 5-phosphate synthase
    Leighanne A Brammer
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Org Lett 11:4748-51. 2009
    ..A study of DXP synthase has revealed flexibility in the acceptor substrate binding pocket for nonpolar substrates and has uncovered new details of the catalytic mechanism to show that pyruvate can act as both donor and acceptor substrate...
  26. pmc Identification and characterization of the smbA gene, a suppressor of the mukB null mutant of Escherichia coli
    K Yamanaka
    Department of Molecular Cell Biology, Kumamoto University School of Medicine, Japan
    J Bacteriol 174:7517-26. 1992
    ..The smbA mutants are sensitive to a detergent, sodium dodecyl sulfate, and they show a novel morphological phenotype under nonpermissive conditions, suggesting a defect in specific membrane sites...
  27. ncbi The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p
    D Karaoglu
    Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0103, USA
    J Biol Chem 272:32513-20. 1997
    ..These experiments, taken together with genetic and biochemical evidence for subunit interactions, suggest that the enzyme is composed of the following three subcomplexes: (a) Stt3p-Ost4p-Ost3p, (b) Swp1p-Wbp1p-Ost2p, and (c) Ost1p-Ost5p...
  28. ncbi Sulfhydryl modification of the yeast Wbp1p inhibits oligosaccharyl transferase activity
    R Pathak
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125, USA
    Biochemistry 34:4179-85. 1995
    ....
  29. ncbi A mutant pyruvate dehydrogenase E1 subunit allows survival of Escherichia coli strains defective in 1-deoxy-D-xylulose 5-phosphate synthase
    Susanna Sauret-Güeto
    Departament de Bioquimica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona Av Diagonal 645, 08028 Barcelona, Spain
    FEBS Lett 580:736-40. 2006
    ....
  30. ncbi The non-mevalonate pathway of isoprenoid precursor biosynthesis
    William N Hunter
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Scotland, UK
    J Biol Chem 282:21573-7. 2007
    ..Detailed knowledge of the pathway may also be exploited to genetically modify microorganisms and plants to produce compounds of agricultural and medical interest...
  31. pmc Staphylococcus aureus deficient in lipidation of prelipoproteins is attenuated in growth and immune activation
    Hartmut Stoll
    Mikrobielle Genetik, Universitat Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Infect Immun 73:2411-23. 2005
    ..Bacterial lipoproteins appear to be essential for a complete immune stimulation by gram-positive bacteria...
  32. ncbi Crystal structure of 1-deoxy-D-xylulose 5-phosphate synthase, a crucial enzyme for isoprenoids biosynthesis
    Song Xiang
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    J Biol Chem 282:2676-82. 2007
    ..The structures identify residues that may have important roles in catalysis, which have been confirmed by our mutagenesis studies...
  33. ncbi Elicitor induced activation of the methylerythritol phosphate pathway toward phytoalexins biosynthesis in rice
    Atsushi Okada
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Tokyo, Japan
    Plant Mol Biol 65:177-87. 2007
    ..These results suggest that activation of the MEP pathway is required to supply sufficient terpenoid precursors for the production of phytoalexins in infected rice plants...
  34. pmc Analysis of the expression of CLA1, a gene that encodes the 1-deoxyxylulose 5-phosphate synthase of the 2-C-methyl-D-erythritol-4-phosphate pathway in Arabidopsis
    J M Estevez
    Departamento de Biologia Molecular de Plantas, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Avenida Universidad 2001 Chamilpa, Apdo Postal 510 3 Cuernavaca Morelos 62271, Mexico
    Plant Physiol 124:95-104. 2000
    ..Microscopic analysis also shows a pleiotropic effect of the CLA1 gene mutation in mesophyll tissue formation...
  35. doi Knock-down of the MEP pathway isogene 1-deoxy-D-xylulose 5-phosphate synthase 2 inhibits formation of arbuscular mycorrhiza-induced apocarotenoids, and abolishes normal expression of mycorrhiza-specific plant marker genes
    Daniela S Floss
    Abteilung Sekundärstoffwechsel, Leibniz Institut für Pflanzenbiochemie IPB, Weinberg 3, D 06120 Halle Saale, Germany
    Plant J 56:86-100. 2008
    ..They further validate the concept of a distinct role for DXS2 in secondary metabolism, and offer a novel tool to selectively manipulate the levels of secondary isoprenoids by targeting their precursor supply...
  36. ncbi Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis
    Kasem Asawatreratanakul
    Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Japan
    Eur J Biochem 270:4671-80. 2003
    ..These results suggest that the Hevea cis-prenyltransferase might require certain activation factors in the washed bottom fraction particles for the production of high molecular mass rubber...
  37. ncbi Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production
    S W Kim
    Marine Bioproducts Engineering Center, Department of Chemical Engineering, University of California, Berkeley, CA 94720 1462, USA
    Biotechnol Bioeng 72:408-15. 2001
    ..A comparison of the three E. coli strains transformed with the arabinose-inducible dxs on a medium-copy plasmid revealed that lycopene production was highest in XL1-Blue...
  38. pmc Host defenses against Staphylococcus aureus infection require recognition of bacterial lipoproteins
    Juliane Bubeck Wardenburg
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 103:13831-6. 2006
    ..Thus, lipoproteins appear to play distinct, nonredundant roles in pathogen recognition and host innate defense mechanisms against S. aureus infections...
  39. ncbi 1-Deoxy-D-xylulose-5-phosphate synthase, a limiting enzyme for plastidic isoprenoid biosynthesis in plants
    J M Estevez
    Departamento de Biologia Molecular de Plantas, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico
    J Biol Chem 276:22901-9. 2001
    ..Furthermore, since the product of the MEP pathway is isopentenyl diphosphate, our results suggest that in plastids the pool of isopentenyl diphosphate is limiting to isprenoid production...
  40. doi Synthesis of the flavonoid-induced lipopolysaccharide of Rhizobium Sp. strain NGR234 requires rhamnosyl transferases encoded by genes rgpF and wbgA
    Silvia Ardissone
    Universite de Geneve, Geneve, Switzerland
    Mol Plant Microbe Interact 24:1513-21. 2011
    ..Two putative rhamnosyl transferases are encoded in a cluster of genes previously shown to be necessary for the synthesis of the rhamnose-rich LPS...
  41. ncbi Arbuscular mycorrhizal fungi induce the non-mevalonate methylerythritol phosphate pathway of isoprenoid biosynthesis correlated with accumulation of the 'yellow pigment' and other apocarotenoids
    M H Walter
    Leibniz Institut für Pflanzenbiochemie, Abteilung Sekundärstoffwechsel, Weinberg 3, D 06120 Halle Saale, Germany
    Plant J 21:571-8. 2000
    ..This is the first report demonstrating (i) that the plastidic MEP pathway is active in plant roots and (ii) that it can be induced by a fungus...
  42. ncbi Identification, cloning, purification, and enzymatic characterization of Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate synthase
    Ann Marie Bailey
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Glycobiology 12:813-20. 2002
    ..The K(d) (thiamin diphosphate )for the native M. tuberculosis DXS activity partially purified from M. tuberculosis cytosol is 1 microM in the presence of Mg(2+)...
  43. doi The isogene 1-deoxy-D-xylulose 5-phosphate synthase 2 controls isoprenoid profiles, precursor pathway allocation, and density of tomato trichomes
    Heike Paetzold
    Leibniz Institut für Pflanzenbiochemie, Abteilung Sekundärstoffwechsel, Weinberg 3, D 06120 Halle Saale, Germany
    Mol Plant 3:904-16. 2010
    ..The results reveal novel, non-redundant roles of DXS2 in modulating isoprenoid metabolism and a pronounced plasticity in isoprenoid precursor allocation...
  44. ncbi Differential expression of three 1-deoxy-D: -xylulose-5-phosphate synthase genes in rice
    Bo Ra Kim
    School of Agricultural Biotechnology, Seoul National University, 151 742, Seoul, Korea
    Biotechnol Lett 27:997-1001. 2005
    ..The differential expression of three OsDXS genes suggested their distinct and complementary roles in the control of the first step of the MEP pathway in response to environmental stimuli...
  45. doi The 1-deoxy-D: -xylulose 5-phosphate synthase gene co-localizes with a major QTL affecting monoterpene content in grapevine
    Juri Battilana
    Department of Genetics and Molecular Biology, IASMA Research Center, Fondazione Edmund Mach, 38010 San Michele all Adige, Italy
    Theor Appl Genet 118:653-69. 2009
    ..Further research on the functional significance of these associations might help to understand the genetic control of Muscat flavor...
  46. ncbi Co-expression of three MEP pathway genes and geraniol 10-hydroxylase in internal phloem parenchyma of Catharanthus roseus implicates multicellular translocation of intermediates during the biosynthesis of monoterpene indole alkaloids and isoprenoid-derive
    Vincent Burlat
    EA 2106, Plant Biocompounds and Biotechnology, UFR des Sciences et Techniques, Universite de Tours, 37200 Tours, France
    Plant J 38:131-41. 2004
    ..Similarly, the translocation of intermediates from the phloem parenchyma is probably also required during the biosynthesis of hormones and photosynthetic primary metabolites derived from the MEP pathway...
  47. pmc Subfunctionality of hydride transferases of the old yellow enzyme family of flavoproteins of Pseudomonas putida
    Pieter van Dillewijn
    Departamento de Protección Ambiental, Estación del Zaidín, Consejo Superior de Investigaciones Cientificas, Granada, Spain
    Appl Environ Microbiol 74:6703-8. 2008
    ..The condensations of the primary products of type I and type II hydride transferases react with each other to yield diarylamines and nitrite; the latter can be further reduced to ammonium and ..
  48. ncbi Studies on the function of oligosaccharyl transferase subunits. Stt3p is directly involved in the glycosylation process
    Qi Yan
    Department of Biochemistry and Cell Biology and the Institute for Cell and Developmental Biology, State University of New York at Stony Brook, Stony Brook, New York 11794 5215, USA
    J Biol Chem 277:47692-700. 2002
    ..From all of these results, we conclude that the sequence from residues 516 to 520, WWDYG in Stt3p, plays a central role in glycosylatable peptide recognition and/or the catalytic glycosylation process...
  49. ncbi Molecular analysis of cis-prenyl chain elongating enzymes
    Yugesh Kharel
    Institute Multidisciplinary Research for Advanced Materials, Tohoku University, Katahira 2 1 1, Aoba ku, Sendai 980 8577, Japan
    Nat Prod Rep 20:111-8. 2003
    ..Not only the primary structure but also the crystal structure of the cis-prenyltransferase is totally different from those of trans-prenyl chain elongating enzymes. This review covers up to February 2002 and contains 72 references...
  50. pmc Cloning and characterization of 1-deoxy-D-xylulose 5-phosphate synthase from Streptomyces sp. Strain CL190, which uses both the mevalonate and nonmevalonate pathways for isopentenyl diphosphate biosynthesis
    T Kuzuyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    J Bacteriol 182:891-7. 2000
    ..To compare the enzymatic properties of CL190 and E. coli DXP synthases, the latter enzyme was also overexpressed and purified. Although these two enzymes had different origins, they showed the same enzymatic properties...
  51. pmc Plastid cues posttranscriptionally regulate the accumulation of key enzymes of the methylerythritol phosphate pathway in Arabidopsis
    Susanna Sauret-Güeto
    Departament de Bioquimica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain
    Plant Physiol 141:75-84. 2006
    ..Regulation of the MEP pathway by a mechanism dependent on plastid cues might function under physiological conditions to finely adjust plastidial isoprenoid biosynthesis to the metabolic capabilities or requirements of plastids...
  52. doi The expression of 1-deoxy-D-xylulose synthase and geraniol-10-hydroxylase or anthranilate synthase increases terpenoid indole alkaloid accumulation in Catharanthus roseus hairy roots
    Christie A M Peebles
    Department of Bioengineering, Rice University, Houston, TX, USA
    Metab Eng 13:234-40. 2011
    ..These results point to the need for overexpressing multiple genes within the pathway to increase the flux toward vinblastine and vincristine...
  53. ncbi Biosynthesis of endotoxins. Purification and catalytic properties of 3-deoxy-D-manno-octulosonic acid transferase from Escherichia coli
    C J Belunis
    Department of Biochemistry, Merck Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
    J Biol Chem 267:9988-97. 1992
    ..In summary, Kdo transferase consists of a single bifunctional polypeptide that incorporates the 2 innermost Kdo residues common to all lipopolysaccharide molecules in E. coli...
  54. ncbi Functional identification and differential expression of 1-deoxy-D-xylulose 5-phosphate synthase in induced terpenoid resin formation of Norway spruce (Picea abies)
    Michael A Phillips
    Max Planck Institut für Chemische Okologie, Abteilung Biochemie, Hans Knöll Str 8, Jena, 07745, Germany
    Plant Mol Biol 65:243-57. 2007
    ..polonica. Our results suggest distinct functions of the three DXS genes in primary and defensive terpenoid metabolism in Norway spruce...
  55. ncbi Allosteric regulation provides a molecular mechanism for preferential utilization of the fully assembled dolichol-linked oligosaccharide by the yeast oligosaccharyltransferase
    D Karaoglu
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0103, USA
    Biochemistry 40:12193-206. 2001
    ..We propose that binding of either donor substrate to the activator site substantially enhances Glc(3)Man(9)GlcNAc(2)-PP-Dol occupancy of the enzyme catalytic site via allosteric activation...
  56. doi In the absence of Lgt, lipoproteins are shed from Streptococcus uberis independently of Lsp
    E L Denham
    Institute for Animal Health, Compton, Berkshire RG20 7NN, UK
    Microbiology 155:134-41. 2009
    ..In such strains, however, the cell-associated MtuA represented the full-length gene product, indicating that Lsp was able to cleave non-lipidated (lipo)proteins but was not responsible for their release from this bacterium...
  57. pmc Up-regulation of 1-deoxy-D-xylulose-5-phosphate synthase enhances production of essential oils in transgenic spike lavender
    Jesús Muñoz-Bertomeu
    Departamento de Biologia Vegetal, Universidad de Valencia, 46100 Burjasot, Valencia, Spain
    Plant Physiol 142:890-900. 2006
    ..In addition, our results provide a strategy to increase the essential oil production in spike lavender by metabolic engineering of the MEP pathway without apparent detrimental effects on plant development and fitness...
  58. ncbi Investigation of factors influencing production of the monocyclic carotenoid torulene in metabolically engineered Escherichia coli
    Pyung Cheon Lee
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 1479 Gortner Avenue, St Paul 55108, USA
    Appl Microbiol Biotechnol 65:538-46. 2004
    ..Lycopene was efficiently converted into torulene during aerobic cultures, indicating that the engineered torulene synthesis pathway is well coordinated, and maintains the functionality and integrity of the carotenogenic enzyme complex...
  59. ncbi Purification and characterization of the bacterial MraY translocase catalyzing the first membrane step of peptidoglycan biosynthesis
    Ahmed Bouhss
    Enveloppes Bactériennes et Antibiotiques, Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR 8619 CNRS, Universite Paris Sud, Bat 430, 91405 Orsay, France
    J Biol Chem 279:29974-80. 2004
    ..The kinetic parameters and effects of pH, salts, cations, and detergents on enzyme activity are described, taking the Bacillus subtilis MraY translocase as a model...
  60. ncbi Lipoproteins are critical TLR2 activating toxins in group B streptococcal sepsis
    Philipp Henneke
    Center for Pediatrics and Adolescent Medicine, University Medical Centre Freiburg, Mathildenstrasse 1, Freiburg, Germany
    J Immunol 180:6149-58. 2008
    ..Notably, LTA comprised little, if any, inflammatory potency when extracted from Deltalgt GBS. In conclusion, mature BLPs, and not LTA, are the major TLR2 activating factors from GBS and significantly contribute to GBS sepsis...
  61. pmc A family of transketolases that directs isoprenoid biosynthesis via a mevalonate-independent pathway
    B M Lange
    Institute of Biological Chemistry, Washington State University, Pullman, WA 99164 6340, USA
    Proc Natl Acad Sci U S A 95:2100-4. 1998
    ..The enzyme appears to represent a novel class of highly conserved transketolases and likely plays a key role in the biosynthesis of plastid-derived isoprenoids essential for growth, development, and defense in plants...
  62. ncbi Lipid modification of bacterial prolipoprotein. Transfer of diacylglyceryl moiety from phosphatidylglycerol
    K Sankaran
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 4799
    J Biol Chem 269:19701-6. 1994
    ..The revised pathway for the lipoprotein biogenesis in bacteria consists of three successive reactions catalyzed by prolipoprotein diacylglyceryl transferase, signal peptidase II, and apolipoprotein N-acyltransferase...
  63. ncbi A fluorometric assay for the determination of 1-deoxy-D-xylulose 5-phosphate synthase activity
    J Querol
    Departament de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, Marti Franquès, 1, Barcelona, 08028, Spain
    Anal Biochem 296:101-5. 2001
    ..This assay can be useful for the functional characterization of DXS as well as for the screening of DXS inhibitors with potential antibiotic, herbicidal, or antimalarial action...
  64. pmc Endotoxin of Neisseria meningitidis composed only of intact lipid A: inactivation of the meningococcal 3-deoxy-D-manno-octulosonic acid transferase
    Yih Ling Tzeng
    Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30303, USA
    J Bacteriol 184:2379-88. 2002
    ..The expression of a fully acylated, unglycosylated lipid A indicates that lipid A biosynthesis in N. meningitidis can proceed without the addition of Kdo and that KdtA is not essential for survival of the meningococcus...
  65. pmc Biosynthesis of selenocysteine on its tRNA in eukaryotes
    Xue Ming Xu
    Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Biol 5:e4. 2007
    ..Conservation of the overall pathway of Sec biosynthesis suggests that this pathway is also active in other eukaryotes and archaea that synthesize selenoproteins...
  66. ncbi Regulation of rubber biosynthetic rate and molecular weight in Hevea brasiliensis by metal cofactor
    Bernardo M T da Costa
    Department of Chemical Engineering, University of California, Berkeley, California 94720, USA
    Biomacromolecules 6:279-89. 2005
    ..The results suggest that H. brasiliensis could use [Mg(2+)] as a regulatory mechanism for rubber biosynthesis and molecular weight in vivo...
  67. pmc DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase
    D J Kelleher
    Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, MA 01655 0103, USA
    Proc Natl Acad Sci U S A 94:4994-9. 1997
    ..The demonstration that DAD1 is a subunit of the OST suggests that induction of a cell death pathway upon loss of DAD1 in the tsBN7 cell line reflects the essential nature of N-linked glycosylation in eukaryotes...
  68. ncbi Retention of subunits of the oligosaccharyltransferase complex in the endoplasmic reticulum
    J Fu
    Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA
    J Biol Chem 275:3984-90. 2000
    ..In the case of the Tac chimera containing only the luminal domain of RII, which by itself exits from the ER and is rapidly degraded, it is retained in the ER and becomes stabilized when coexpressed with OST48...
  69. ncbi Structural and functional investigation of a putative archaeal selenocysteine synthase
    Jens T Kaiser
    Division of Chemistry and Chemical Engineering, California Institute of Technology, M C 114 96, Pasadena, California 91125, USA
    Biochemistry 44:13315-27. 2005
    ....
  70. ncbi Selenocysteine synthase from Escherichia coli. Nucleotide sequence of the gene (selA) and purification of the protein
    K Forchhammer
    Lehrstuhl fur Mikrobiologie, Universitat Munchen, Federal Republic of Germany
    J Biol Chem 266:6318-23. 1991
    ..Evidence is presented that the overall conversion of seryl-tRNA(Sec UCA) to selenocysteyl-tRNA(Sec UCA) occurs at the SELA protein. SELA, therefore, has the function of a selenocysteine synthase...
  71. pmc The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis
    M Sato
    Molecular Membrane Biology Laboratory, RIKEN, Wako, Saitama 351 0198, Japan
    Mol Cell Biol 19:471-83. 1999
    ..The difference in phenotypes between the rer2 mutant and previously obtained glycosylation mutants suggests a novel, as-yet-unknown role of dolichol...
  72. ncbi Selenocysteine synthase from Escherichia coli. Analysis of the reaction sequence
    K Forchhammer
    Lehrstuhl fur Mikrobiologie, Universitat Munchen, Federal Republic of Germany
    J Biol Chem 266:6324-8. 1991
    ....
  73. pmc Prenylation of Rab8 GTPase by type I and type II geranylgeranyl transferases
    A L Wilson
    Hood Research Program, Weis Center for Research, Pennsylvania State University College of Medicine, 100 N Academy Avenue, Danville, PA 17822 2616, USA
    Biochem J 333:497-504. 1998
    ..Prenylation of the Y78D mutant was reduced by 60-70% in intact cells, consistent with the conclusion that the majority of Rab8 is prenylated by the REP/GGTaseII system in vivo...
  74. ncbi Genetically encoded short peptide tags for orthogonal protein labeling by Sfp and AcpS phosphopantetheinyl transferases
    Zhe Zhou
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    ACS Chem Biol 2:337-46. 2007
    ..as efficient substrates for site-specific protein labeling catalyzed by Sfp and AcpS phosphopantetheinyl transferases (PPTases), respectively...
  75. ncbi Selenoprotein synthesis in E. coli. Purification and characterisation of the enzyme catalysing selenium activation
    A Ehrenreich
    Lehrstuhl fur Mikrobiologie, Universitat Munchen, Federal Republic of Germany
    Eur J Biochem 206:767-73. 1992
    ..The alpha,beta cleavage of ATP is also supported by the finding that neither adenosine 5'-[alpha,beta-methylene]triphosphate nor adenosine 5'-[beta,gamma-methylene]triphosphate served as substrates in the reaction...
  76. ncbi Lipoproteins of Listeria monocytogenes are critical for virulence and TLR2-mediated immune activation
    Silke Machata
    Institute of Medical Microbiology, Justus Liebig University, Giessen, Germany
    J Immunol 181:2028-35. 2008
    ....
  77. ncbi Targeting the MraY and MurG bacterial enzymes for antimicrobial therapeutic intervention
    Laura E Zawadzke
    Pharmaceutical Research Institute, Bristol Myers Squibb, Wallingford, CT 06492 7660, USA
    Anal Biochem 314:243-52. 2003
    ..A mureidomycin inhibitor mix was used as a control for the assay development and screen validation. Several inhibitors resulting from a high-throughput screen were found and evaluated for potential therapeutic use...
  78. pmc Active translocon complexes labeled with GFP-Dad1 diffuse slowly as large polysome arrays in the endoplasmic reticulum
    Andrei V Nikonov
    Department of Cell Biology, New York University School of Medicine, New York 10016, USA
    J Cell Biol 158:497-506. 2002
    ..Our findings that TCs assembled into membrane-bound polysomes diffuse slowly within the ER have mechanistic implications for the segregation of the ER into smooth and rough domains...
  79. ncbi Activation and inhibition of rubber transferases by metal cofactors and pyrophosphate substrates
    Deborah J Scott
    USDA ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA
    Phytochemistry 64:123-34. 2003
    ..All three RuTs have similar characteristics-indeterminate sized products, high K(m)(IPP), high metal [A](max), metal cofactor requirements, and are membrane-bound enzymes...
  80. pmc Type II hydride transferases from different microorganisms yield nitrite and diarylamines from polynitroaromatic compounds
    Pieter van Dillewijn
    Departamento de Protección Ambiental, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Granada, Spain
    Appl Environ Microbiol 74:6820-3. 2008
    ..reductase of Enterobacter cloacae, and N-ethylmaleimide reductase of Escherichia coli, all type II hydride transferases of the Old Yellow Enzyme family of flavoproteins, are shown to reduce the polynitroaromatic compound 2,4,6-..
  81. ncbi A novel 3-deoxy-D-manno-octulosonic acid transferase from Chlamydia trachomatis required for expression of the genus-specific epitope
    C J Belunis
    Department of Biochemistry, Merck Research Laboratories, Rahway, New Jersey 07065
    J Biol Chem 267:18702-7. 1992
    ..trachomatis enzyme is at least trifunctional. Given the similarities of these two Kdo transferases and the essentiality of Kdo in Gram-negative bacteria, lipopolysaccharide biosynthesis may be a target for ..
  82. doi Role of prolipoprotein diacylglyceryl transferase (Lgt) and lipoprotein-specific signal peptidase II (LspA) in localization and physiological function of lipoprotein MsmE in Streptococcus mutans
    T Arimoto
    Department of Oral Microbiology, Showa University School of Dentistry, Tokyo, Japan
    Oral Microbiol Immunol 23:515-9. 2008
    ..mutans...
  83. ncbi Thiamin-dependent enzymes as catalysts in chemoenzymatic syntheses
    U Schörken
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, P O Box 1913, D 52425 Julich, Germany
    Biochim Biophys Acta 1385:229-43. 1998
    ....
  84. ncbi The function of selenocysteine synthase and SELB in the synthesis and incorporation of selenocysteine
    K Forchhammer
    Lehrstuhl fur Mikrobiologie, Universitat Munchen, Germany
    Biochimie 73:1481-6. 1991
    ..tRNA complex. Mechanistically, this provides the biochemical basis for the exclusive selection of selenocysteyl-tRNA(Sec) in the decoding step of a selenocysteine-specific UGA triplet...
  85. pmc Scintillation proximity assay for inhibitors of Escherichia coli MurG and, optionally, MraY
    Sudha Ravishankar
    AstraZeneca India Pvt Ltd, Hebbal, Bellary Rd, Bangalore 560 024, India
    Antimicrob Agents Chemother 49:1410-8. 2005
    ..In addition, it inhibited NADH dehydrogenase in membranes, a hitherto-unreported activity. These assays can be used to screen for novel antibacterial agents...
  86. ncbi Polyprenyl lipid synthesis in mammalian cells expressing human cis-prenyl transferase
    Jullian Jones
    Department of Chemical and Biomolecular Engineering, Baltimore, MD 21218, USA
    Biochem Biophys Res Commun 331:379-83. 2005
    ..These results suggest a regulatory relationship between CPT activity and dolichol biosynthesis, and may implicate CPT in the levels of dolichol-oligosaccharide intermediate biosynthesis...
  87. ncbi Structural homology among mammalian and Saccharomyces cerevisiae isoprenyl-protein transferases
    N E Kohl
    Department of Cancer Research, Merck Sharp and Dohme Research Laboratories, West Point, Pennsylvania 19486
    J Biol Chem 266:18884-8. 1991
    ....
  88. ncbi The Ost1p subunit of yeast oligosaccharyl transferase recognizes the peptide glycosylation site sequence, -Asn-X-Ser/Thr-
    Q Yan
    Department of Biochemistry and Cell Biology and the Institute for Cell and Developmental Biology, SUNY at Stony Brook, Stony Brook, New York 11794, USA
    J Biol Chem 274:5021-5. 1999
    ..We conclude that Ost1p is the subunit of the OST complex that recognizes the peptide sites in the nascent chains that are destined to be glycosylated...
  89. ncbi The highly conserved DAD1 protein involved in apoptosis is required for N-linked glycosylation
    T Makishima
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Higashi ku, Fukuoka, Japan
    Genes Cells 2:129-41. 1997
    ..It has a mutation in the DAD1 gene encoding a 12.5kDa highly conserved protein through evolution, and enters apoptosis at the restrictive temperature due to this mutation...
  90. ncbi Effects of TGF-beta on hyaluronan anabolism in fibroblasts derived from the synovial membrane of the rabbit temporomandibular joint
    K Tanimoto
    Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    J Dent Res 83:40-4. 2004
    ..These results suggest that TGF-beta 1 enhances the expression of HAS2 mRNA in the TMJ synovial membrane fibroblasts and may contribute to the production of high-molecular-weight HA in the joint fluid...
  91. ncbi Hyaluronan--magic glue for the regulation of the immune response?
    Christian Termeer
    Department of Dermatology, University of Freiburg, Hauptstrasse 7, D 79104 Freiburg, Germany
    Trends Immunol 24:112-4. 2003
  92. ncbi 'Piggy-back' transport of Xenopus hyaluronan synthase (XHAS1) via the secretory pathway to the plasma membrane
    Johannes Müllegger
    Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstr 11, A 5020 Salzburg, Austria
    Biol Chem 384:175-82. 2003
    ..From there the inactive protein is transferred to the plasma membrane via the secretory pathway. For unknown reasons, HAS inserted into membranes other than the plasma membrane remains inactive...
  93. pmc Abnormal accumulation of hyaluronan matrix diminishes contact inhibition of cell growth and promotes cell migration
    Naoki Itano
    Institute for Molecular Science of Medicine, Aichi Medical University, Nagakute, Aichi 480 1195, Japan
    Proc Natl Acad Sci U S A 99:3609-14. 2002
    ..Our observations suggest that hyaluronan and its matrix can modulate contact inhibition of cell growth and migration, and provide evidence for functional differences between hyaluronan synthesized by the different HAS proteins...
  94. ncbi Expression of hyaluronan synthases and CD44 messenger RNAs in porcine cumulus-oocyte complexes during in vitro maturation
    Naoko Kimura
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Aoba ku, Sendai 981 8555, Japan
    Biol Reprod 66:707-17. 2002
    ....
  95. ncbi An enzyme capture assay for analysis of active hyaluronan synthases
    Zhetcho Kyossev
    Department of Biochemistry and Molecular Biology and the Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
    Anal Biochem 371:62-70. 2007
    ..The HAS capture assay was also used to assess the fraction of HAS molecules that were active, which cannot be done using standard assays for synthase activity. Assay sensitivity for detection of purified SeHAS is <1 pmol...
  96. pmc The luxS gene of Streptococcus pyogenes regulates expression of genes that affect internalization by epithelial cells
    Mehran J Marouni
    Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
    Infect Immun 71:5633-9. 2003
    ..Our results indicate that LuxS activity in strain SP268 plays an important role in the expression of virulence factors associated with epithelial cell internalization...
  97. ncbi Alteration of polysaccharide size distribution of a vertebrate hyaluronan synthase by mutation
    Philip E Pummill
    Department of Biochemistry and Molecular Biology, Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City 73104, USA
    J Biol Chem 278:19808-14. 2003
    ..These data support the theory that the relative strength of the interaction between the catalyst and the polymer may be a major factor in HA size control...
  98. ncbi Expression cloning and molecular characterization of HAS protein, a eukaryotic hyaluronan synthase
    N Itano
    Institute for Molecular Science of Medicine, Aichi Medical University, Yazako, Nagakute, Aichi 480 11, Japan
    J Biol Chem 271:9875-8. 1996
    ..Therefore, HAS cDNA is responsible for the activity of the hyaluronan synthase, a key enzyme of hyaluronan synthesis in eukaryotic cells...
  99. ncbi Molecular identification of a putative human hyaluronan synthase
    K Watanabe
    Burnham Institute, California 92037, USA
    J Biol Chem 271:22945-8. 1996
    ..These results suggest that the Has2 protein is a crucial component of the human hyaluronan synthase system...
  100. ncbi Molecular cloning of human hyaluronan synthase
    N Itano
    Institute for Molecular Science of Medicine, Aichi Medical University, Japan
    Biochem Biophys Res Commun 222:816-20. 1996
    ..4% and 96.0% identity in nucleotide sequence and amino acid sequences, respectively. Northern analysis indicated that the gene was ubiquitously expressed in various human tissues...
  101. ncbi Characterization and molecular evolution of a vertebrate hyaluronan synthase gene family
    A P Spicer
    Department of Biochemistry and Molecular Biology, Mayo Clinic Scottsdale, Arizona 85259, USA
    J Biol Chem 273:1923-32. 1998
    ..We propose that vertebrate hyaluronan biosynthesis is regulated by a comparatively ancient gene family that has arisen by sequential gene duplication and divergence...

Research Grants101 found, 100 shown here

  1. Correction of glutathione deficiency for treatment of diabetic nephropathy
    Paolo Fanti; Fiscal Year: 2013
    ..enzymes participating in GSH metabolism, including GSH-reductase (GSH-R), GSH-peroxidase (GSH-Px), GSH-S-transferases (GST) and superoxide dismutase (SOD)...
  2. The gastric X/A-like (ghrelin) cell: A peripheral regulator of food intake
    NILS WG LAMBRECHT; Fiscal Year: 2012
    ..The proposed studies will address important pathophysiological questions regarding the mechanisms regulating appetite and satiety as well as provide potentially important clinical treatment strategies. ..
  3. Enterococcus faecalis, Colorectal Cancer, and Bystander Effects
    Mark M Huycke; Fiscal Year: 2012
    ..BSO) and N-acetyl-L-cysteine (NAC) as inhibitors or promoters, respectively, of glutathione and glutathione S-transferases will be tested...
  4. Mitochondrial Dysfunction and Adipose Insulin Resistance
    David A Bernlohr; Fiscal Year: 2013
    ..If the hypothesis is proven to be correct, the study would be immediately translatable to human biology and afford a new view of how type 2 diabetes may be combated. ..
  5. Preclinical Development of JS-K, a Novel NO-Generating Prodrug for Cancer
    Paul J Shami; Fiscal Year: 2012
    ..This new drug then goes one step further: it will improve the quality of life for cancer patients, their caregivers and families by providing a significantly more effective and less painful therapy than is currently available. ..
  6. Prevention of UV-induced carcinogenesis by cyanidin-3-glucoside
    Xianglin Shi; Fiscal Year: 2013
    ..Present study attempts to identify cyanidin-3-glucoside, a plant-derived compound, as a mechanism-based preventive agent against UV-induced skin cancers. ..
  7. Arsenic Trioxide and Acute Myeloid Leukemia
    Yongkui Jing; Fiscal Year: 2012
    ..Our goal will be to utilize As2O3 in combination with 4 HPR or EA analogs in clinical trials in patients with refractory, secondary or in elderly patients with acute myelogenous leukemia. ..
  8. Ceramide Metabolism and Photoreceptor Homeostasis
    Usha R Acharya; Fiscal Year: 2013
    ....
  9. Roles for microRNA-122 in hepatitis C virus RNA amplification
    Peter Sarnow; Fiscal Year: 2013
    ..Deep sequencing analysis and gene knockdown of suspected nucleotidyl transferases will aid in this analysis...
  10. MECHANISM-BASED DRUG SELECTION AND DESIGN: NUCLEOTIDE SA
    Buddy Ullman; Fiscal Year: 2003
    ..Concurrent experiments will determine how NTPase functions in AK HGXPRT-, and AT parasites, identify the protein(s) which regulate NTPase enzymatic activity, and evaluate the impact of abrogating NTPase expression on parasite viability. ..
  11. Role of Glutathione S-Transferase P in Heart Failure
    Sumanth D Prabhu; Fiscal Year: 2013
    ..Glutathione S-transferases (GSTs) metabolize aldehydes by catalyzing their conjugation with glutathione (GSH)...
  12. Bioflavonoid Effects on EGF Signaling and Cell Cycle Pathways in Prostate Cancer
    BARRY MATTHEW MARKAVERICH; Fiscal Year: 2013
    ..Specific coregulators (histone acetyl transferases;HAT's and histone deacetylases;HDAC's) recruited to EGFR, c-Fos, PLK1 and CDKN1B promoters will be ..
  13. Regulation of LPS-mediated HMGB1 Release by Poly (ADP-ribose) Polymerase-1
    RAJESH K ANEJA; Fiscal Year: 2013
    ..Additional studies will be performed to determine the therapeutic window for use of PARP-1 inhibitors in murine sepsis. ..
  14. Mechanisms of Stem Cell Self-Renewal and Aging
    PAUL CHRISTIAN SCHILLER; Fiscal Year: 2013
    ..Aim 3: Determine the role of progerin in the bone forming capacity of hMSCs in vivo. We expect that increased progerin expression in hMSCs will decrease their bone forming capacity in vivo. ..
  15. Mitochondrial Dysfunction and Adipose Insulin Resistance
    DAVID BERNLOHR; Fiscal Year: 2009
    ..Aim 3. Develop and characterize aP2-HA-GSTA4 transgenic mice maintained on low and high fat diets. Aim 4. Characterize the regulation of insulin signaling by the Trx2-ASK1-JNK pathway in animal and cell culture models...
  16. Genistein and Prostate Cancer
    Rajvir Dahiya; Fiscal Year: 2010
    ..Alterations in gene-specific histone acetylation using ChIP assay, and enzymatic activities of histone acetyl transferases (HATs). 4) Histone deacetylase (HDACs) analysis using biochemical assays. 5)...
  17. N-Acetylcysteine and Milk Thistle for Treatment of Diabetic Nephropathy
    Paolo Fanti; Fiscal Year: 2011
    ..enzymes participating in GSH metabolism, including GSH-reductase (GSH-R), GSH-peroxidase (GSH-Px), GSH-S-transferases (GST) and superoxide dismutase (SOD)...
  18. Prolactin as a Growth Factor in Breast Cancer
    Nira Ben-Jonathan; Fiscal Year: 2013
    ..The long term goal is to establish FDA-approved, long acting dopamine agonists as suppressor or PRL secretion, resulting in increased efficacy of chemotherapeutic agents in breast cancer patients. ..
  19. Randomized trial of PEITC as a modifier of NNK metabolism in smokers
    Jian Min Yuan; Fiscal Year: 2012
    ..The major strengths include the need for a randomized, placebo-controlled trial of PEITC, the qualification of the investigators, and the feasibility of the proposed studies. ..
  20. Fic-mediated Adenylylation
    CAROLYN ANN WORBY; Fiscal Year: 2013
    ..This covalent addition of AMP blocks signal transduction pathways in the host that are important for combating bacterial infections. ..
  21. Nuclear Receptor Coactivator Functions and Mechanisms
    Robert G Roeder; Fiscal Year: 2013
    ..coactivator functions, histone modifying factors such as the activating p300 acetyl- and SET1/MLL methyl-transferases, and other DNA-binding regulatory factors (C/EBPs) that act synergistically with PPAR?...
  22. Mitochondrial Oxidative Stress in the Retinal Pigment Epithelium as a Model for A
    Alfred S Lewin; Fiscal Year: 2013
    ..oxidative stress by elevating a set of antioxidant enzymes, including heme oxygenase-1, glutathione transferases, and NAD(P)H:quinone oxidoreductase 1...
  23. Protein Modifications and Unfolded Protein Response in Diabetic Embryopathy
    Zhiyong Zhao; Fiscal Year: 2013
    ..In Specific Aim 3, we will investigate whether TRAF1 triggers DISC formation by binding to TRADD and other DISC factors. We will, then, investigate whether TRAF1 causes NTDs in diabetic embryopathy using a traf1 knockout model. ..
  24. Epidemiological Research on Autism in Jamaica
    Mohammad H Rahbar; Fiscal Year: 2010
    ....
  25. Nuclear Events in PTH Action on Bone
    Nicola C Partridge; Fiscal Year: 2013
    ..In the nucleus, Runx2 then recruits the histone acetyl transferases (HATs), p300 and p300/CBP associated factor (PCAF)...
  26. Contribution of Gstm1 to the severity of hypertension and chronic kidney disease
    Thu H Le; Fiscal Year: 2013
    ..of GSTM1 is glutathione S-transferase m-1, or GSTM1 enzyme, that belongs to a superfamily of glutathione-S-transferases that metabolize xenobiotics and a broad range of reactive oxygen species (ROS), and the highly reactive ..
  27. Investigating the pathogenesis of CoQ10 deficiencies
    Catarina M Quinzii; Fiscal Year: 2013
    ..Knowing the cause of CoQ10 deficiency will likely enhance our scientific knowledge of CoQ10 biosynthesis, and will provide molecular tests for accurate genetic counseling, prenatal diagnosis, and more rapid initiation of the therapy. ..
  28. Mitochondrial function and antifungal photodynamic therapy
    Constantine G Haidaris; Fiscal Year: 2011
    ..targeted is linked to maintenance of mitochondrial redox balance by thioredoxins, glutaredoxins and glutathione transferases. Specific Aim 3 will determine whether Candida mutants in mitochondrial redox pathways display increased ..
  29. Mechanisms of tubular atrophy in renal disease
    Jeffrey R Schelling; Fiscal Year: 2012
    ..The purpose of this project is to determine why kidneys fail. In particular, we plan to study biochemical pathways that prevent kidneys cells from dying, which may lead to new therapies to halt the progression of kidney diseases. ..
  30. The Role of hPso4 in DNA Repair and Chemotherapy Resistance
    Montaser Shaheen; Fiscal Year: 2013
    ..We will deplete this E2 and evaluate if this will achieve the same chemosensitivity as depleting hPso4. This will open the opportunity to identify Ubc3 small molecule inhibitors as cancer therapy. ..
  31. Androgen Receptor-JunD Complex Inhibitors to Prevent Prostate Cancer Progression
    Hirak S Basu; Fiscal Year: 2012
    ..The most active agent, thus identified, can be further developed for preclinical and clinical testing to prevent progression of early-stage androgen-dependent PCa to CRPCa. ..
  32. Genetic Determinants of Lung Injury
    Beverly H Koller; Fiscal Year: 2013
    ....
  33. MECHANISMS OF LIVER INJURY BY HEPATITIS C AND ALCOHOL
    Steven A Weinman; Fiscal Year: 2013
    ..These PTMs result from altered activity of upstream kinases, acetyl transferases, deacetylases, ubiquitin conjugating enzymes and deubiquitinases...
  34. Epidemiological Research on Autism in Jamaica - Phase II
    Mohammad H Rahbar; Fiscal Year: 2013
    ....
  35. Chemoprevention of Tamoxifen-induced Endometrial cancer by black cohosh and red c
    BIRGIT MARIA DIETZ; Fiscal Year: 2010
    ..We hypothesize that red clover and black cohosh, both frequently used for the alleviation of menopausal symptoms, will reduce tamoxifen-induced endometrial cancer due to their cancer chemopreventive properties. ..
  36. Epigenetic regulation of liver transporters
    Frederick J Suchy; Fiscal Year: 2013
    ..These studies will provide insight into an unexplored area of hepatic biology and pathobiology, and suggest potential new targets for drug development. ..
  37. C/EBP, atrogin-1, and muscle wasting
    Per Olof Hasselgren; Fiscal Year: 2009
    ..Improved insight into mechanisms accounting for muscle wasting will help develop better treatments of this debilitating condition. ..
  38. Diesel, Allergens and Gene Interaction and Child Atopy
    Grace LeMasters; Fiscal Year: 2009
    ....
  39. Study on the intracellular Network of TBX3
    Taosheng Huang; Fiscal Year: 2010
    ..Elucidation of the TBX3- HDAC interaction will deepen our understanding of the functions of TBX3 and may also lead to the identification of a novel therapeutic target for breast cancer. ..
  40. Genetic Susceptibility Factors in Porphyria Cutanea Tarda (PCT)
    Karl Anderson; Fiscal Year: 2009
    ..that polymorphisms in phase I and phase II enzymes, such as cytochrome P450 enzymes (CYPs) and glutathione transferases (GSTs) may contribute to developing PCT...
  41. Cigarette Smoke-derived Electrophilic Aldehydes and Airway Inflammation
    Albert van Der Vliet; Fiscal Year: 2013
    ....
  42. Factors for Epigenetic Silencing of Lung Cancer Genes
    Steven Belinsky; Fiscal Year: 2009
    ..Finally we will describe the gene-specific promoter hypermethylation patterns at study entry and at 18-month follow-up. ..
  43. Enzymology of Post-translational Modifications
    Carol A Fierke; Fiscal Year: 2013
    ..Palmitoylation is catalyzed by protein palmitoyl transferases (PATs), such as Akr1p, and is a thioester exchange reaction between palmitoyl-CoA and a cysteine on a substrate ..
  44. Regulation of Hepatic Gluconeogenesis by the CREB:TORC2 Pathway
    Marc R Montminy; Fiscal Year: 2013
    ..By characterizing these chemical changes in TORC2 and understanding how they modify the ability for this switch to trigger glucose production, our studies may lead to new therapies for the treatment of diabetic patients. ..
  45. Regulation of Hepatic Gluconeogenesis by the CREB:TORC2 Pathway
    Marc R Montminy; Fiscal Year: 2010
    ..By characterizing these chemical changes in TORC2 and understanding how they modify the ability for this switch to trigger glucose production, our studies may lead to new therapies for the treatment of diabetic patients. ..
  46. Impact of genetic variation on response to GO therapy in COG-AML clinical trials
    ROLAND BRUNO WALTER; Fiscal Year: 2013
    ..induction chemotherapy, we now propose to test our hypothesis that SNPs in CD33, ABCB1, SOCS3, glutathione-S-transferases, DNA-repair and DNA -damage response pathway genes (XRCC4/5, XPC, PARP1, LIG4, ATM, and ATR), and apoptosis-..
  47. Noncoding RNAs as epigenomic modulators in Alzheimer's Disease
    CLAES ROBERT WAHLESTEDT; Fiscal Year: 2010
    ..However, these genetic causes are at least in part atypical (nonconventional), and we therefore argue that so-called "epigenetic/epigenomic" causes must be considered. ..
  48. Detoxification of Electrophilic Chemical Threat Agents by Nucleophilic Scavengers
    Michael C MacLeod; Fiscal Year: 2010
    ..In addition, we will obtain data that indicates whether or not individuals with variants of a particular class of genes, the GSTs, are more susceptible to the effects of SNM toxicants. ..
  49. Chemistry, Structure and Biology of Thiol Transferases
    Richard N Armstrong; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Thiols, thiol-transferases and related proteins are essential to virtually all aspects of biology...
  50. ROS, Inflammation, and Cardioprotection in Type 2 Diabetes
    Rakesh C Kukreja; Fiscal Year: 2013
    ..Overall, the proposed studies will pave the way to a potentially novel pharmacotherapy against inflammation and cardiac injuries resulting from T2D conditions. ..
  51. Cardioprotective Mechanisms of Glutathione S-transferase P
    DANIEL JOSEPH CONKLIN; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): The glutathione S-transferases (GSTs) are a superfamily of ubiquitously expressed enzymes that conjugate reduced glutathione (GSH) with toxic electrophiles...
  52. Design of CNS-Permeable Agents for the Treatment of Lipid Storage Diseases
    Scott D Larsen; Fiscal Year: 2010
    ..the inhibition of glycosphingolipid biosynthesis by the use of small molecule inhibitors of glycosphingolipid transferases, most notably glucosylceramide synthase...
  53. Systemic Chemotherapy of Melanoma: NMR Studies of Lonidamine &N-Mustard Activity
    Jerry D Glickson; Fiscal Year: 2013
    ..3) To evaluate potential 1H NMR markers for noninvasive early detection of response--lactate and total choline by MRS and apparent diffusion constant (ADC) and T2 measured by MRI. ..
  54. FACTORS THAT MODIFY INSULIN ACTION
    Maria G Buse; Fiscal Year: 2012
    ..Improved understanding of how different nutrients, when provided chronically in excess, alter the cell's response to insulin, will lead to the development of novel therapeutic targets to prevent or mitigate type 2 diabetes. ..
  55. Novel histone biotinylation sites and relationships to other epigenetic marks
    JANOS contact ZEMPLENI; Fiscal Year: 2010
    ..3) Ongoing projects in our laboratories suggest that HCS interacts with H3 K9-methyl transferases and that K12-biotinylated histone H4 co-localizes with K9- dimethylated histone H3...
  56. Regulation of IGF-1 and Insulin Signaling by O-GlcNAc Glycosylation
    LAUREN ELIZABETH BALL; Fiscal Year: 2013
    ....
  57. Molecular Pathogenesis of Coenzyme Q10 Deficiency
    Michio Hirano; Fiscal Year: 2012
    ..Aim 4: In collaboration with Professor Pl[unreadable]cido Navas, University of Sevilla, Spain, we will characterize CoQ10 biosynthetic genes and mutations in yeast. ..
  58. Effects of Dietary Fat on Contractile and Mitochondrial Function in Heart Failure
    Margaret P Chandler; Fiscal Year: 2010
    ....
  59. Ozone Cardiovascular Effects in Genetically Susceptible People
    MARK WALTER FRAMPTON; Fiscal Year: 2012
    ..Understanding the effects of ozone on the heart and circulation can help establish appropriate air pollution standards, and provide strategies to protect the most susceptible people. ..
  60. GLYCOSYLATION MUTANTS OF LEISHMANIA
    Salvatore J Turco; Fiscal Year: 2012
    ..2. To characterize new families of mannosyl-phosphate transferases and galactosyltransferases involved in synthesis of the phosphoglycan (PG) repeating unit Gal(21,4)Man(11)-PO4- ..
  61. Regulation and Function of Adiponectin Oligomerization
    Feng Liu; Fiscal Year: 2012
    ....
  62. Regulation of NF-kappaB in Lung Epithelium by ROS/RNS
    Yvonne M W Janssen-Heininger; Fiscal Year: 2013
    ..Completion of this application will provide new avenues to limit allergic inflammation, and also provide new insights into the anti-inflammatory mechanism of GSTP, which is essentially unknown. ..
  63. Protein Kinase C Signaling Mechanisms in Cancer
    Alan P Fields; Fiscal Year: 2013
    ....
  64. Regulation of AKAP79 Postsynaptic Targeting and Signaling
    MARK L DELL'ACQUA; Fiscal Year: 2013
    ..synaptic localization by palmitoyl-acyl transferases (PATs) focusing on DHHC2...
  65. Lectin Resistant Tumor Cells And Functional Glycomics
    PAMELA M STANLEY; Fiscal Year: 2013
    ..The molecular basis of the requirement for MGAT1 will be investigated by candidate and unbiased approaches. ..
  66. Pharmacokinetic and Pharmacogenomic Determinants of Cisplatin Kidney Injury
    Melanie S Joy; Fiscal Year: 2013
    ..transporters (OCT2/SLC22A2, MATE1/SLC47A1, and MRP2/ABCC2), glutathione metabolizing enzymes (Glutathione-S-transferases, e.g. GSTP1 and GSTA1, and g-glutamyl transferase1, e.g...
  67. O-GlcNAc Modification in Metabolic Homeostasis
    Xiaoyong Yang; Fiscal Year: 2013
    ..Pharmacological intervention to reset O-GlcNAc levels may represent an innovative strategy for preventing and treating metabolic syndrome. ..
  68. Developmental Pharmacology of cytosolic and mitochondrial GSTZ1-1/MAAI
    PETER WALLACE STACPOOLE; Fiscal Year: 2013
    ....
  69. GENETIC ANALYSIS OF SYNAPSE FORMATION AND FUNCTION
    Kendal Broadie; Fiscal Year: 2013
    ....
  70. Host protein prenyl transferases as modifiers of effectors of pathogenic bacteria
    ELIA WRIGHT; Fiscal Year: 2013
    ..Knowledge of how effecto proteins are modified by eukaryotic host cells may lead to the identification of novel pathways that could be exploited for the development of antibacterials. ..
  71. Targeting the lipid synthesis enzyme, DGAT1, for breast cancer prevention
    IVAN PETER URAY; Fiscal Year: 2013
    ..The results of these studies will provide the preclinical foundation to develop effective and safe ways to prevent all forms of breast cancer in humans. ..
  72. Down-regulation of oxidant-induced airway inflammation though modulation of NRF2
    Michelle Hernandez; Fiscal Year: 2013
    ..Angela Kashuba, the director f the clinical pharmacology fellowship at UNC with extensive experience in clinical pharmacokinetic and pharmacodynamic studies. ..
  73. DNA methylation in aging, race and prostate cancer
    Rajvir Dahiya; Fiscal Year: 2006
    ..This information can be used for better management of prostate cancer using DNA methylated genes as diagnostic or prognostic biomarkers. ..
  74. Effects of Acitretin on the activation of latent HIV
    Peilin Li; Fiscal Year: 2013
    ..latency through effects on chromatin structure, a process controlled by two families of enzymes: histone acetyl-transferases (HAT), and histone deacetylases (HDAC)...
  75. Transcriptional Regulation of Motor Neuron Subtype Development
    MADALYNN LEA ERB; Fiscal Year: 2013
    ..These experiments will be the first unbiased examination of motor neuron subtype specific gene expression in developing embryos. ..
  76. TGF Beta Signals in Prostate Stromal-Epithelial Interactions
    Neil A Bhowmick; Fiscal Year: 2013
    ....
  77. Structure determination of reaction intermediates in macromolecular complexes
    Amie K Boal; Fiscal Year: 2013
    ..abstract_text> ..
  78. Advanced GST Proteomics for Early Stage Organ-Specific Toxicity Screening. Phase
    Kevin M Patrie; Fiscal Year: 2013
    ....
  79. The role of decapping factors in mRNP remodeling
    MARC ARRIBAS-LAYTON; Fiscal Year: 2013
    ..The targets of the Hat1-Rbbp7 complex will be identified and the role of their acetylation in the mRNP decay process will be studied through mutational analysis and Hat1-Rbbp7 depletion. ..
  80. Antipsychotic and Folate Pharmacogenetics
    Vicki L Ellingrod; Fiscal Year: 2013
    ..The primary positive impact of our anticipated findings will be an evidence-based scientific framework for folate intervention development for AAP metabolic complications, which can be directly translated into clinical practice. ..
  81. Aberrant N-Glycan Standards for Mass Spectrometry and Microarray Analysis
    ANTHONY PRUDDEN; Fiscal Year: 2013
    ..of chemo-enzymatic methods in which a synthetic oligosaccharide precursor is modified by a range of glycosyl transferases to give a more complex compound...
  82. Non-nucleoside inhibitors of DNA methyl transferase I
    Nigel D Priestley; Fiscal Year: 2013
    ..In this application we seek to demonstrate that we can develop potent and selective inhibitors of Dnmt-1. We further seek to demonstrate that our inhibitors cause reactivation of a model epigenetically silenced gene, human MLH1. ..
  83. Structures and Functions of RNA Editing TUTases
    RUSLAN AFASIZHEV; Fiscal Year: 2013
    ..We discovered three Terminal Uridylyl Transferases (TUTases), enzymes of unique structures and essential functions...
  84. Pathogenesis and Treatment of NUT-Midline Carcinoma
    Christopher A French; Fiscal Year: 2013
    ..blocks differentiation by sequestering chromatin "writers" necessary for transcription, such as histone acetyl- transferases (HATs), away from genes required for differentiation to limited genomic regions where the bromodomains of BRD "..
  85. Chemical Approaches to Understand the Roles of O-GlcNAc Glycosylation in Biology
    Linda C Hsieh-Wilson; Fiscal Year: 2013
    ....
  86. Understanding role of O-GlcNAcylation on cancer cell metabolism and survival
    CHRISTINA FERRER; Fiscal Year: 2013
    ..These studies will further our understanding of how metabolic reprogramming in cancer cells connects at the molecular level to survival stress pathways and identify novel anticancer pathways. ..
  87. SBIR PHASE II: SITE-SPECIFIC ANTIBODIES FOR GLCNACYLATED PROTEINS IN CANCER
    Hyesook Kim; Fiscal Year: 2013
    ..abstract_text> ..
  88. OGlcNAc as a regulator of mitochondrial motility in diabetic neuropathy
    Cathy Su; Fiscal Year: 2013
    ..Overall, the completion of the proposal will provide broad insight on the role of mitochondria in axon degeneration and neuropathy as well as further characterization of a novel regulatory role of the O-GlcNAc modification. ..
  89. LIPID PEROXIDATION IN COLORECTAL CANCER
    Manuela Gago Dominguez; Fiscal Year: 2009
    ..4. To conduct a case-case association study to determine if morphological markers of apoptosis in tumor and surrounding free tissue differ among cases with varying exposure levels of a given putative environmental/genetic risk factor. ..
  90. Role of nutrient sensor O-GlcNAc transferase in regulating cancer
    Mauricio J Reginato; Fiscal Year: 2013
    ..These studies will further our understanding of how metabolic reprogramming in cancer cells connects at the molecular level to cell growth and survival pathways and identify novel anticancer agents. ..
  91. NEW CHEMICAL APPROACHES TO ADP-RIBOSYL TRANSFER
    James Slama; Fiscal Year: 1991
    ADP-ribosyl transferases are an important class of regulatory enzymes. This project proposes the design and affinity labels specific for the NAD substrate binding site of ADP-ribosyl transferases...
  92. Targeting MELK-mediated EZH2 signaling in glioma stem cells
    Ichiro Nakano; Fiscal Year: 2013
    ..We anticipate that this study will yield a new paradigm for GSC biology and a novel therapeutic approach to target key regulators of GSC, which may lead to the translation into improved therapies. ..
  93. GLYCOPROTEIN HORMONE OLIGOSACCHARIDES
    Jacques U Baenziger; Fiscal Year: 2010
    ..The beta1,4-N-acetylgalactosaminyl-transferases ( betaGTs) that add GalNAc to this structure are protein-selective, recognizing specific amino acid sequences ..
  94. ORAL CANCER--DNA POLYMORPHISMS IN CARCINOGEN METABOLISM
    Stephen Schwartz; Fiscal Year: 2001
    ....
  95. A C. ELEGANS MODEL FOR NMNAT1-MEDIATED HYPOXIC PROTECTION AND LIFESPAN EXTENSION
    C Michael Crowder; Fiscal Year: 2013
    ..Completion of these aims will develop a powerful new model to study this important enzyme and may define a mechanism whereby Nmnat1 protects from hypoxia and lengthens lifespan. ..
  96. The Biochemistry and Biology of C. elegans Poly(U) Polymerases
    Melanie A Preston; Fiscal Year: 2013
    ..PUPs belong to the family of beta-nucleotidyl transferases that act on RNA (rNTRs), which includes noncanonical poly(A) polymerases (PAPs)36...
  97. Halogenated Alkenes and Microsomal GSH-transferases
    Michael J Kelner; Fiscal Year: 2010
    ..Complicating the issue is the recent finding that there are multiple human microsomal glutathione transferases capable of conjugating halogenated hydrocarbons...
  98. O-GLYCOSYLATION OF HCG AND CANCER
    Laurence Cole; Fiscal Year: 1993
    ....
  99. BRAIN GLUTATHIONE S-TRANSFERASES AND NEUROTOXICITY
    Jeffrey Johnson; Fiscal Year: 2000
    ..The overall goal of my laboratory is to evaluate the regulation and cell-specific expression of glutathione S-transferases (GSTs) in neural cells and the potential role of GSTs in prevention of neurotoxicity...
  100. MOLECULAR ANALYSIS OF MUCIN TYPE O GLYCOSYLATION
    Michael Hollingsworth; Fiscal Year: 2000
    The cDNAs for three human UDP-N-Acetylgalactosamine; Polypeptide N- Acetylgalactosaminyltransferases (GaINAc transferases) have recently been cloned and sequenced...
  101. Retinyl Ester Binding Proteins and the Visual Cycle
    Robert Rando; Fiscal Year: 2007
    ..unreadable] [unreadable]..