Research Topics

Genomes and Genes

Species

transposases

Summary

Summary: Enzymes that recombine DNA segments by a process which involves the formation of a synapse between two DNA helices, the cleavage of single strands from each DNA helix and the ligation of a DNA strand from one DNA helix to the other. The resulting DNA structure is called a Holliday junction which can be resolved by DNA REPLICATION or by HOLLIDAY JUNCTION RESOLVASES.

Top Publications

  1. ncbi piggyBac can bypass DNA synthesis during cut and paste transposition
    Rupak Mitra
    Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
    EMBO J 27:1097-109. 2008
  2. ncbi Transposases are the most abundant, most ubiquitous genes in nature
    Ramy K Aziz
    Computation Institute, University of Chicago, Chicago, IL 60637, USA
    Nucleic Acids Res 38:4207-17. 2010
  3. ncbi Chromosomal transposition of PiggyBac in mouse embryonic stem cells
    Wei Wang
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 105:9290-5. 2008
  4. ncbi Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates
    Lajos Mátés
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Genet 41:753-61. 2009
  5. ncbi Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition
    Andrew Adey
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Genome Biol 11:R119. 2010
  6. ncbi Messenger RNA as a source of transposase for sleeping beauty transposon-mediated correction of hereditary tyrosinemia type I
    Andrew Wilber
    The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 15:1280-7. 2007
  7. ncbi Stable integration and conditional expression of electroporated transgenes in chicken embryos
    Yuki Sato
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara, 630 0192, Japan
    Dev Biol 305:616-24. 2007
  8. ncbi Pig transgenesis by Sleeping Beauty DNA transposition
    Jannik E Jakobsen
    Department of Human Genetics, University of Aarhus, Wilh Meyers Allé 1240, 8000, Aarhus C, Denmark
    Transgenic Res 20:533-45. 2011
  9. ncbi A transposon and transposase system for human application
    Perry B Hackett
    Department of Genetics, Cell Biology, and Development, Center for Genome Engineering, Institute of Human Genetics, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 18:674-83. 2010
  10. ncbi Unexpectedly high copy number of random integration but low frequency of persistent expression of the Sleeping Beauty transposase after trans delivery in primary human T cells
    Xin Huang
    Pediatric Blood and Marrow Transplantation, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Hum Gene Ther 21:1577-90. 2010

Research Grants

  1. BRUCELLA VACCINE FOR BIOTERRORISM
    Gary Splitter; Fiscal Year: 2004
  2. GENE AMPLIFICATION BY A TRANSPOSITION APPARATUS
    Hiroshi Nakai; Fiscal Year: 1999
  3. Genetic transformation system for Chlamydia suis
    DANIEL ROCKEY; Fiscal Year: 2007
  4. Plant Transposases and their MITEs
    Susan Wessler; Fiscal Year: 2007
  5. A gene-trap screen for hearing and balance
    DONNA FEKETE; Fiscal Year: 2007
  6. TRANSPOSASES IN ETOPOSIDE RESISTANCE
    Robert A Hromas; Fiscal Year: 2010
  7. MECHANISTIC ANALYSIS OF DNA TRANSPOSITION BY PHAGE MU
    Tania Baker; Fiscal Year: 2001
  8. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    ADRIENNE COTTRELL; Fiscal Year: 2007
  9. MUTATOR TRANSPORTABLE ELEMENTS OF MAIZE
    Virginia Walbot; Fiscal Year: 2005
  10. MOLECULAR ANALYSIS OF THE INSERTION ELEMENT IS903
    KEITH DERBYSHIRE; Fiscal Year: 2004

Detail Information

Publications193 found, 100 shown here

  1. ncbi piggyBac can bypass DNA synthesis during cut and paste transposition
    Rupak Mitra
    Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
    EMBO J 27:1097-109. 2008
    ..This is the same strategy for target joining used by the members of DDE superfamily of transposases and retroviral integrases...
  2. ncbi Transposases are the most abundant, most ubiquitous genes in nature
    Ramy K Aziz
    Computation Institute, University of Chicago, Chicago, IL 60637, USA
    Nucleic Acids Res 38:4207-17. 2010
    ..archaeal, eukaryotic and viral genomes and metagenomes, and our analysis demonstrates that genes encoding transposases are the most prevalent genes in nature...
  3. ncbi Chromosomal transposition of PiggyBac in mouse embryonic stem cells
    Wei Wang
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 105:9290-5. 2008
    ..The comprehensive information from this study should facilitate further exploration of the potential of PB and SB DNA transposons in mammalian genetics...
  4. ncbi Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates
    Lajos Mátés
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Genet 41:753-61. 2009
    ....
  5. ncbi Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition
    Andrew Adey
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Genome Biol 11:R119. 2010
    ..We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing...
  6. ncbi Messenger RNA as a source of transposase for sleeping beauty transposon-mediated correction of hereditary tyrosinemia type I
    Andrew Wilber
    The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 15:1280-7. 2007
    ....
  7. ncbi Stable integration and conditional expression of electroporated transgenes in chicken embryos
    Yuki Sato
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara, 630 0192, Japan
    Dev Biol 305:616-24. 2007
    ..Thus, the techniques proposed in this study provide a novel approach to study the mechanisms of late organogenesis, for which chickens are most suitable model animals...
  8. ncbi Pig transgenesis by Sleeping Beauty DNA transposition
    Jannik E Jakobsen
    Department of Human Genetics, University of Aarhus, Wilh Meyers Allé 1240, 8000, Aarhus C, Denmark
    Transgenic Res 20:533-45. 2011
    ....
  9. ncbi A transposon and transposase system for human application
    Perry B Hackett
    Department of Genetics, Cell Biology, and Development, Center for Genome Engineering, Institute of Human Genetics, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 18:674-83. 2010
    ..This review examines the rationale and safety implications of application of the SB system to genetically modify T cells to be manufactured in compliance with current good manufacturing practice (cGMP) for phase I/II trials...
  10. ncbi Unexpectedly high copy number of random integration but low frequency of persistent expression of the Sleeping Beauty transposase after trans delivery in primary human T cells
    Xin Huang
    Pediatric Blood and Marrow Transplantation, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Hum Gene Ther 21:1577-90. 2010
    ..Our data suggest that SB delivery via plasmid in T cells should be carried out with caution because of unexpectedly high copy numbers of randomly integrated SB transposase...
  11. ncbi Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transfer
    Andrew Wilber
    The Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Stem Cells 25:2919-27. 2007
    ..We conclude that the SB transposon system provides an effective approach with several advantages for genetic manipulation and durable gene expression in human ES cells...
  12. ncbi A transposon toolkit for gene transfer and mutagenesis in protozoan parasites
    Jeziel D Damasceno
    Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil
    Genetica 138:301-11. 2010
    ..The piggyBac and Tn5 transposons have also been shown to mobilize across Plasmodium spp. genomes circumventing the current limitations in the genetic manipulation of these organisms...
  13. ncbi Delivering the goods: viral and non-viral gene therapy systems and the inherent limits on cargo DNA and internal sequences
    Helen Atkinson
    School of Biomedical Sciences, University of Nottingham, Queen s Medical Center, Nottingham NG7 2UH, UK
    Genetica 138:485-98. 2010
    ..Length-dependence is a complex phenomenon driven not simply by the distance between the transposon ends, but by host proteins, the transposase and the properties of the DNA sequences encoded within the transposon...
  14. ncbi Identification and characterization of repetitive extragenic palindromes (REP)-associated tyrosine transposases: implications for REP evolution and dynamics in bacterial genomes
    Jaroslav Nunvar
    Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic
    BMC Genomics 11:44. 2010
    ..BIMEs and REPs have been shown to serve as binding sites for several proteins and suggested to play role in chromosome organization and transcription termination. Their origins are, at present, unknown...
  15. ncbi Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP/Cre recombinase system to distinguish transposition-specific expression
    Paul R Score
    Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 13:617-24. 2006
    ..The results also provide additional evidence for the effectiveness of the Sleeping Beauty transposon system as an in vivo DNA-mediated gene transfer strategy for achieving long-term expression...
  16. ncbi Resetting the site: redirecting integration of an insertion sequence in a predictable way
    Catherine Guynet
    Laboratoire de Microbiologie et Genetique Moleculaires, CNRS UMR5100, 118 Rte de Narbonne, F31062 Toulouse Cedex, France
    Mol Cell 34:612-9. 2009
    ..This suggested a way to redirect IS608 transposition to novel target sites. As we demonstrate here, we can now direct insertions in a predictable way into a variety of different chosen target sequences, both in vitro and in vivo...
  17. ncbi Transposon-free insertions for insect genetic engineering
    George C Condon
    Oxitec Limited, 71 Milton Park, Oxford OX14 4RX, UK
    Nat Biotechnol 24:820-1. 2006
    ..We describe a method for post-integration elimination of all transposon sequences in the pest insect Medfly, Ceratitis capitata. The resulting insertions lack transposon sequences and are therefore impervious to transposase activity...
  18. ncbi Efficient mammalian germline transgenesis by cis-enhanced Sleeping Beauty transposition
    Daniel F Carlson
    The Center for Genome Engineering, Minneapolis, MN, USA
    Transgenic Res 20:29-45. 2011
    ..Given its simplicity, versatility and high efficiency, TnT with enhanced Sleeping Beauty components represents a compelling non-viral approach to modifying the mammalian germline...
  19. ncbi Hermes transposon distribution and structure in Musca domestica
    Ramanand A Subramanian
    Center for Biosystems Research, University of Maryland Biotechnology Institute, Rockville, MD 20850, USA
    J Hered 100:473-80. 2009
    ..The simple sequence repeat found in hobo elements is a feature of this transposon that evolved since the divergence of hobo and Hermes...
  20. ncbi Transposition of the human Hsmar1 transposon: rate-limiting steps and the importance of the flanking TA dinucleotide in second strand cleavage
    Corentin Claeys Bouuaert
    School of Biomedical Sciences, University of Nottingham, Queen s Medical Centre, Nottingham, UK
    Nucleic Acids Res 38:190-202. 2010
    ..Furthermore, this conformational change requires specific contacts between transposase and the flanking TA dinucleotide...
  21. ncbi A functional role for transposases in a large eukaryotic genome
    Mariusz Nowacki
    Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA
    Science 324:935-8. 2009
    ..transposase genes play key roles in this process of genome-wide DNA excision, which suggests that transposases function in large eukaryotic genomes containing thousands of active transposons...
  22. ncbi Chimeric recombinases with designed DNA sequence recognition
    Aram Akopian
    Institute of Biomedical and Life Sciences, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, Scotland
    Proc Natl Acad Sci U S A 100:8688-91. 2003
    ..Our results demonstrate the functional autonomy of the resolvase catalytic domain and open the way to creating "custom-built" recombinases that act at chosen natural target sequences...
  23. ncbi Post-integration behavior of a Mos1 mariner gene vector in Aedes aegypti
    Raymond Wilson
    Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742-4450, USA
    Insect Biochem Mol Biol 33:853-63. 2003
    ..aegypti for applications requiring a very high degree of vector stability but will have limited use in the construction of genetic drive, enhancer trap, or transposon tagging systems in this species...
  24. ncbi Active site sharing and subterminal hairpin recognition in a new class of DNA transposases
    Donald R Ronning
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 20:143-54. 2005
    ..Protein sequences of IS605 transposases do not bear the hallmarks of other well-characterized transposases...
  25. ncbi Transposons for cancer gene discovery: Sleeping Beauty and beyond
    Lara S Collier
    The Department of Genetics, Cell Biology and Development, The Cancer Center, The University of Minnesota Twin Cities, Church St SE, Minneapolis, Minnesota 55455, USA
    Genome Biol 8:S15. 2007
    ..Here, we discuss the future of Sleeping Beauty for cancer genetic studies and the potential use of additional transposable elements for somatic mutagenesis...
  26. ncbi Sleeping beauty transposition from nonintegrating lentivirus
    Conrad A Vink
    Institute of Child Health, University College London, UK
    Mol Ther 17:1197-204. 2009
    ..Importantly, integration site analysis revealed redirection toward a profile mimicking SB-plasmid integration and away from integration within transcriptionally active genes favored by integrase-proficient lentiviral vectors (ILVs)...
  27. ncbi Multiple oligomerisation domains in the IS911 transposase: a leucine zipper motif is essential for activity
    L Haren
    Laboratoire de Microbiologie et Genetique Moleculaire du CNRS, Universite Paul Sabatier, 118 route de Narbonne, Toulouse, 31062, France
    J Mol Biol 283:29-41. 1998
    ..The two other regions are located further towards the C-terminal end of the protein, adjacent to the leucine zipper and in the region that carries the conserved catalytic DD(35)E motif...
  28. ncbi Hybrid lentivirus-transposon vectors with a random integration profile in human cells
    Nicklas H Staunstrup
    Department of Human Genetics, University of Aarhus, Denmark
    Mol Ther 17:1205-14. 2009
    ..The novel hybrid vector system combines the attractive features of efficient gene delivery by viral transduction and a safer genomic integration profile by DNA transposition...
  29. ncbi High-level genomic integration, epigenetic changes, and expression of sleeping beauty transgene
    Jianhui Zhu
    Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Biochemistry 49:1507-21. 2010
    ..In addition, long-range activation of host genes must now be recognized as a potential consequence of an inserted transgene cassette containing enhancer elements...
  30. ncbi Translating Sleeping Beauty transposition into cellular therapies: victories and challenges
    Zsuzsanna Izsvák
    Max Delbrück Center for Molecular Medicine MDC, Berlin, Germany
    Bioessays 32:756-67. 2010
    ..The first clinical application of the SB system will help to validate the safety of this approach...
  31. ncbi Shielding of sleeping beauty DNA transposon-delivered transgene cassettes by heterologous insulators in early embryonal cells
    Trine Dalsgaard
    Department of Human Genetics, University of Aarhus, Aarhus, Denmark
    Mol Ther 17:121-30. 2009
    ..We believe that insulated SB-derived vectors will become useful tools in transposon-based transgenesis and therapeutic gene transfer...
  32. ncbi DNA recognition sites activate MuA transposase to perform transposition of non-Mu DNA
    Ilana Goldhaber-Gordon
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 277:7694-702. 2002
    ..Multiple recognition sites help physically position MuA subunits in the transpososome plus each individual site allosterically activates transposase...
  33. ncbi Sequence and positional requirements for DNA sites in a mu transpososome
    Ilana Goldhaber-Gordon
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 277:7703-12. 2002
    ..This variability causes only local perturbations in the protein-DNA complex, as indicated by experiments in which altered and unaltered DNA substrates are paired...
  34. ncbi The N-terminus of Himar1 mariner transposase mediates multiple activities during transposition
    Matthew G Butler
    Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA
    Genetica 127:351-66. 2006
    ..we are beginning to understand the precise mechanism of transposition of these elements, the structure of their transposases are still poorly understood...
  35. ncbi The mariner transposons belonging to the irritans subfamily were maintained in chordate genomes by vertical transmission
    Ludivine Sinzelle
    , CNRS UMR 8080, IBAIC, , , Orsay Cedex, F-91405, France
    J Mol Evol 62:53-65. 2006
    ..The phylogenetic analysis of the relationships between MLE transposases reveals that Xtmar1 is closely related to Hsmar2 and Bytmar1 and that together they form a second distinct ..
  36. ncbi Transposon-based interferon gamma gene transfer overcomes limitations of episomal plasmid for immunogene therapy of glioblastoma
    A Wu
    Department of Neurosurgery, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Cancer Gene Ther 14:550-60. 2007
    ..SB appears to improve the efficacy of cytokine gene therapy using nonviral vectors by enhancing the duration of transgene expression...
  37. ncbi Generation of an inducible and optimized piggyBac transposon system
    Juan Cadinanos
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
    Nucleic Acids Res 35:e87. 2007
    ..As the activity of PB is higher than that of SB11 and SB12, two hyperactive SB transposases, we have characterized and improved the PB system in mouse ES cells...
  38. ncbi Identification and analysis of a hyperactive mutant form of Drosophila P-element transposase
    Eileen L Beall
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA
    Genetics 162:217-27. 2002
    ..These data suggest that the activity of P-element transposase may be regulated by phosphorylation and demonstrate that one mutation, S129A, results in hyperactive transposition...
  39. ncbi Liver-directed gene therapy using the sleeping beauty transposon system
    Lalitha R Belur
    Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, and Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA
    Methods Mol Biol 434:267-76. 2008
    ....
  40. ncbi Analysis of the piggyBac transposase reveals a functional nuclear targeting signal in the 94 c-terminal residues
    James H Keith
    University of Notre Dame, Notre Dame, Indiana, USA
    BMC Mol Biol 9:72. 2008
    ..A PSORTII analysis of the TPase amino acid sequence predicts a bipartite nuclear localization signal (NLS) near the c-terminus, just upstream of a putative ZnF (ZnF)...
  41. ncbi Abundant transposases encoded by the metagenome of a hydrothermal chimney biofilm
    William J Brazelton
    School of Oceanography and Center for Astrobiology and Early Evolution, University of Washington, Seattle, WA 98195, USA
    ISME J 3:1420-4. 2009
    ..More than 8% of all metagenomic reads showed significant sequence similarity to transposases; all available metagenomic data sets from other environments contained at least an order of magnitude fewer ..
  42. ncbi Mariner Mos1 transposase optimization by rational mutagenesis
    Stephanie Germon
    GICC, Universite Francois Rabelais de Tours, 37200, Tours, France
    Genetica 137:265-76. 2009
    ..Even if host factors are not essential for transposition, our data demonstrate that the host machinery is essential in regulating MOS1 activity...
  43. ncbi DNA recognition and the precleavage state during single-stranded DNA transposition in D. radiodurans
    Alison Burgess Hickman
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    EMBO J 29:3840-52. 2010
    Bacterial insertion sequences (ISs) from the IS200/IS605 family encode the smallest known DNA transposases and mobilize through single-stranded DNA transposition...
  44. ncbi A simple topological filter in a eukaryotic transposon as a mechanism to suppress genome instability
    Corentin Claeys Bouuaert
    School of Biomedical Sciences, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2UH, United Kingdom
    Mol Cell Biol 31:317-27. 2011
    ..This provides constraints on the enzymatic mechanism of the double-strand breaks in mariner transposition, excluding the most prevalent of the current models...
  45. ncbi The mariner Mos1 transposase produced in tobacco is active in vitro
    Xavier Thomas
    Universite Francois Rabelais de Tours, GICC, Parc de Grandmont, 37200 Tours, France
    Genetica 138:519-30. 2010
    ..Several hypotheses are proposed that could explain the inhibition of HMNtMOS1 activity in planta...
  46. ncbi A novel DNA binding and nuclease activity in domain III of Mu transposase: evidence for a catalytic region involved in donor cleavage
    Z Wu
    Department of Biochemistry, University of Western Ontario, London, Canada
    EMBO J 14:3835-43. 1995
    ..This proposal for active site assembly is in agreement with the recently proposed domain sharing model by Yang et al. (Yang, J.Y., Kim, K., Jayaram, M. and Harshey, R.M. [1995] EMBO J., 14, 2374-2384)...
  47. ncbi Transposase-transposase interactions in MOS1 complexes: a biochemical approach
    Guillaume Carpentier
    Universite Francois Rabelais de Tours, GICC, CNRS, UMR 6239, UFR Sciences and Techniques, Parc Grandmont, 37200 Tours, France
    J Mol Biol 405:892-908. 2011
    b>Transposases are proteins that have assumed the mobility of class II transposable elements...
  48. ncbi The catalytic domain of all eukaryotic cut-and-paste transposase superfamilies
    Yao Wu Yuan
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 108:7884-9. 2011
    ..g., hAT, P) based on sequence similarity of the element-encoded transposase. The transposases from several superfamilies possess a protein domain containing an acidic amino acid triad (DDE or DDD) that ..
  49. ncbi In vitro recombination and inverted terminal repeat binding activities of the Mcmar1 transposase
    Sylvaine Renault
    Universite Francois Rabelais de Tours, GICC, CNRS, UMR 6239, UFR des Sciences and Techniques, Parc de Grandmont, 37200 Tours, France
    Biochemistry 49:3534-44. 2010
    ..This suggests that the assembly of the transposition complex is more complex than that of those involved in the mobility of the Mos1 and Himar1 mariner elements...
  50. ncbi Duration of expression and activity of Sleeping Beauty transposase in mouse liver following hydrodynamic DNA delivery
    Jason B Bell
    Department of Genetics, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 18:1796-802. 2010
    ..Thus, within the limits of current technology, we show that SB transposons appear to be as stably integrated as their viral counterparts...
  51. ncbi Transposition of ISHp608, member of an unusual family of bacterial insertion sequences
    Bao Ton-Hoang
    , CNRS UMR5100, Toulouse Cedex, France
    EMBO J 24:3325-38. 2005
    ..We also demonstrate that TnpA, which is less than half the length of other transposases, is responsible for this and for ISHp608 transposition...
  52. ncbi An in vivo transposase-catalyzed single-stranded DNA circularization reaction
    P Polard
    Laboratoire de Microbiologie et Genetique Moleculaires, UPR9007 du Centre National de la Resherche Scientifique CNRS, Toulouse, France
    Genes Dev 9:2846-58. 1995
    ..Therefore, IS911 excision as a circle may not occur by double-strand cleavage leading to its prior separation from the vector backbone in a linear form but could proceed by consecutive circularization of each strand...
  53. ncbi In vitro reconstitution of a single-stranded transposition mechanism of IS608
    Catherine Guynet
    Laboratoire de Microbiologie et Genetique Moleculaires, CNRS UMR5100, 118 Rte de Narbonne, F31062 Toulouse Cedex, France
    Mol Cell 29:302-12. 2008
    ..This single-strand transposition mode has important implications not only for dispersion of IS608 but also for the other members of this very large IS family...
  54. ncbi Conservation of Palindromic and Mirror Motifs within Inverted Terminal Repeats of mariner-like Elements
    Yves Bigot
    Laboratoire d Etude des Parasites Génétiques, Universite Francois Rabelais, E A 3868, UFR des Sciences et Techniques, Parc de Grandmont, Avenue Monge, 37200 Tours, France
    J Mol Biol 351:108-16. 2005
    ..ITR) are therefore recognized, as previously indicated, by biochemical data obtained with the Mos1 and Himar1 transposases. Here, we define the motifs that are involved in the binding of a MLE transposase to its ITR by analyzing the ..
  55. ncbi Microbial co-habitation and lateral gene transfer: what transposases can tell us
    Sean D Hooper
    Department of Energy Joint Genome Institute, Genome Biology Program, Mitchell Drive, Walnut Creek, CA 94598, USA
    Genome Biol 10:R45. 2009
    ..In this study, we explore an approach using the history of lateral gene transfer recorded in microbial genomes to begin to answer two key questions: where have you been and who have you been with?..
  56. ncbi Assembly of the Tc1 and mariner transposition initiation complexes depends on the origins of their transposase DNA binding domains
    Benjamin Brillet
    Laboratoire d Etudes des Parasites Génétiques, Universite Francois Rabelais, FRE CNRS 2969, UFR Sciences and Techniques, Parc Grandmont, 37200, Tours, France
    Genetica 130:105-20. 2007
    ..Elements belonging to this super-family encode transposases with DNA binding domains of different origins, and recent data indicate that the chimerization of functional ..
  57. ncbi Sleeping Beauty transposase modulates cell-cycle progression through interaction with Miz-1
    Oliver Walisko
    Max Delbruck Center for Molecular Medicine, D 13092 Berlin, Germany
    Proc Natl Acad Sci U S A 103:4062-7. 2006
    ..Because nonhomologous end-joining is required for efficient SB transposition, the transposase-induced G(1) slowdown is probably a selfish act on the transposon's part to maximize the chance for a successful transposition event...
  58. ncbi Dissecting Tn5 transposition using HIV-1 integrase diketoacid inhibitors
    Agata Czyz
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Biochemistry 46:10776-89. 2007
    ..Thus, DKA inhibitors will provide an important set of tools to investigate the mechanism of action of transposases and integrases.
  59. ncbi DNA transposons in vertebrate functional genomics
    C Miskey
    , , 13092, Berlin, Germany
    Cell Mol Life Sci 62:629-41. 2005
    ..We put emphasis on the evolution and regulation of members of the best-characterized and most widely used Tc1/mariner family...
  60. ncbi Characterization of multiple lineages of Tc1-like elements within the genome of the amphibian Xenopus tropicalis
    Ludivine Sinzelle
    , CNRS UMR 8080, IBAIC, , , F-91405 Orsay Cedex, France
    Gene 349:187-96. 2005
    ..Xeminos is the first vertebrate TLE strongly related to an invertebrate lineage. Finally, we have identified for most of these TLEs, copies containing an intact transposase ORF suggesting that these elements may still be active...
  61. ncbi Transposons reanimated in mice
    Timothy H Bestor
    Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA
    Cell 122:322-5. 2005
    ..In either case, the future of transposon-driven genetic analyses of mice and other mammals looks promising...
  62. ncbi Family of Tc1-like elements from fish genomes and horizontal transfer
    Anita Pocwierz Kotus
    Department of Genetics and Marine Biotechnology, Institute of Oceanology, Polish Academy of Sciences, Sw Wojciecha 5, 81 347 Gdynia, Poland
    Gene 390:243-51. 2007
    ..Further similar studies would help to explain the evolution of the multiple Tc1 lineages including species for which full genome sequences will not be available soon...
  63. ncbi Gene transfer into genomes of human cells by the sleeping beauty transposon system
    Aron M Geurts
    Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Mol Ther 8:108-17. 2003
    ..With the recently described improvements to the transposon itself, the SB system appears to be a potential gene-transfer tool for human gene therapy...
  64. ncbi Subtraction by addition: domesticated transposases in programmed DNA elimination
    Jason A Motl
    Biology Department, Washington University in St Louis, St Louis, Missouri 63130, USA
    Genes Dev 23:2455-60. 2009
    ..This new study reveals how addition of a transposase to small RNA-directed silencing machinery can guide major genome reorganization...
  65. ncbi Transposition and target specificity of the typical IS30 family element IS1655 from Neisseria meningitidis
    Janos Kiss
    Agricultural Biotechnology Center, Szent Gyorgyi Albert u 4, H 2100, Godollo, Hungary
    Mol Microbiol 63:1731-47. 2007
    ..Moreover, it shows striking similarity to IS30 in transposition strategy even though their transposases differ in their N-terminal regions, which, for IS30, appears to determine target specificity...
  66. ncbi Two-metal active site binding of a Tn5 transposase synaptic complex
    Scott Lovell
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Nat Struct Biol 9:278-81. 2002
    ..The position of the metal ions and the conformational changes of the DNA provide insight into the mechanism of hairpin formation and cleavage, and is consistent with a two-metal model for catalysis...
  67. ncbi Unexpected structural diversity in DNA recombination: the restriction endonuclease connection
    A B Hickman
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 5:1025-34. 2000
    ..This result indicates that the range of biological processes that utilize restriction enzyme-like folds also includes DNA transposition...
  68. ncbi When phage, plasmids, and transposons collide: genomic islands, and conjugative- and mobilizable-transposons as a mosaic continuum
    A Mark Osborn
    Department of Biological Sciences, University of Essex, Colchester, CO4 3SQ, UK
    Plasmid 48:202-12. 2002
    ..This review highlights both the similarities and distinctions between these integrating transferable elements resulting from combination of the MGE toolbox...
  69. ncbi A Coxiella burnetti repeated DNA element resembling a bacterial insertion sequence
    T A Hoover
    Bacteriology Division, U S Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702 5011
    J Bacteriol 174:5540-8. 1992
    ..Despite the sequence changes, all three copies of the element have retained significant dyad symmetry in this region...
  70. ncbi Target DNA structure plays a critical role in RAG transposition
    Jennifer E Posey
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Biol 4:e350. 2006
    ..These findings suggest novel regulatory possibilities and may provide insight into the activities of other transposases.
  71. ncbi Sub-terminal sequences modulating IS30 transposition in vivo and in vitro
    Monika Szabo
    Agricultural Biotechnology Center, 4 Szent Györgyi Albert Str, H 2100, Godollo, Hungary
    J Mol Biol 375:337-52. 2008
    ..In vitro kinetic experiments suggest that the sub-terminal enhancers may, at least partly, be transposase-dependent. Such enhancers may reflect a subtle regulatory mechanism for IS30 transposition...
  72. ncbi Complete sequence determination combined with analysis of transposition/site-specific recombination events to explain genetic organization of IncP-7 TOL plasmid pWW53 and related mobile genetic elements
    Hirokazu Yano
    Graduate School of Life Sciences, Tohoku University, Katahira, Sendai 980 8577, Japan
    J Mol Biol 369:11-26. 2007
    ....
  73. ncbi Diverse Mariner-like elements in fig wasps
    E R Haine
    Division of Biology, Imperial College London, Silwood Park, Ascot, Berkshire, UK
    Insect Mol Biol 16:743-52. 2007
    ..The association between mariner elements and fig wasps is old and dominated by vertical transmission, suggesting that these 'selfish genetic elements' have evolved to impart only very low costs to their hosts...
  74. ncbi ISWpi1 from Wolbachia pipientis defines a novel group of insertion sequences within the IS5 family
    Richard Cordaux
    Laboratoire de Génétique et Biologie des Populations de Crustacés, CNRS UMR 6556, Universite de Poitiers, 40 Avenue du Recteur Pineau, 86022 Poitiers, France
    Gene 409:20-7. 2008
    ..pipientis bacteria, especially in light of the increasing interest in developing these bacteria as tools for controlling insect disease vectors and agricultural pests...
  75. ncbi Mechanism of IS200/IS605 family DNA transposases: activation and transposon-directed target site selection
    Orsolya Barabas
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 132:208-20. 2008
    The smallest known DNA transposases are those from the IS200/IS605 family. Here we show how the interplay of protein and DNA activates TnpA, the Helicobacter pylori IS608 transposase, for catalysis...
  76. ncbi Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes
    Ludivine Sinzelle
    Max Delbruck Center for Molecular Medicine, 13092 Berlin, Germany
    Proc Natl Acad Sci U S A 105:4715-20. 2008
    ..The Harbinger transposon will serve as a useful experimental system for transposon biology and for investigating the enzymatic functions of domesticated, transposon-derived cellular genes...
  77. ncbi Transcribed Tc1-like transposons in salmonid fish
    Aleksei Krasnov
    Institute of Applied Biotechnology, University of Kuopio, P, O, B, 1627, FIN 70211 Kuopio, Finland
    BMC Genomics 6:107. 2005
    ..High throughput sequencing of salmonid fish cDNA libraries has revealed a large number of transposons, which remain transcribed despite inactivation of translation. This article reports on the structure and potential role of these genes...
  78. ncbi In vivo random mutagenesis of Bacillus subtilis by use of TnYLB-1, a mariner-based transposon
    Yoann Le Breton
    Department of Microbiology and Immunology, MSC 7758, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229 3900, USA
    Appl Environ Microbiol 72:327-33. 2006
    ..Two auxotrophic mutants were identified among the 200 transposants, one with an insertion in lysA and another in a gene (yjzB) whose function is unknown...
  79. ncbi Translocation of transposition-deficient (TndPKLH2-like) transposons in the natural environment: mechanistic insights from the study of adjacent DNA sequences
    Gennady Kholodii
    Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia
    Microbiology 150:979-92. 2004
    ..On the basis of DNA sequence data, possible mechanisms of translocation of defective Tn(d)PKLH2-like elements via recombination events implicating the nearby res (resolution) site and IS element are proposed...
  80. ncbi Congruence of in vivo and in vitro insertion patterns in hot E. coli gene targets of transposable element Mu: opposing roles of MuB in target capture and integration
    Jun Ge
    Section of Molecular Genetics and Microbiology and Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    J Mol Biol 380:598-607. 2008
    ..Actual integration events are then directed to sites that are in proximity to MuB filaments but are themselves free of MuB...
  81. ncbi Characterization of a small mobilizable transposon, MTnSag1, in Streptococcus agalactiae
    Adeline Achard
    CHU Cote de Nacre, Universite de Caen Basse Normandie, 14033 Caen Cedex, France
    J Bacteriol 189:4328-31. 2007
    ..MTnSag1 could be mobilized by the conjugative transposon Tn916. An intermediate circular form of MTnSag1 and a putative origin of transfer at the 3' end of the lnu(C) gene were characterized...
  82. ncbi Chimeric Mos1 and piggyBac transposases result in site-directed integration
    K J Maragathavally
    Department of Entomology, Texas A and M University, College Station, Texas, USA
    FASEB J 20:1880-2. 2006
    ..We developed a novel technology that utilizes chimeric transposases to direct integration into specific sites on a target DNA molecule...
  83. ncbi Mos1-mediated insertional mutagenesis in Caenorhabditis elegans
    Thomas Boulin
    ENS, 46 rue d Ulm, F 75005 Paris, France
    Nat Protoc 2:1276-87. 2007
    ..The recent development of targeted engineering of C. elegans loci carrying Mos1 insertions further increases the attractiveness of Mos1-mediated mutagenesis...
  84. ncbi Tol2 transposon-mediated transgenesis in Xenopus tropicalis
    Michelle R Johnson Hamlet
    Department of Pathology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
    Genesis 44:438-45. 2006
    ..Here, we show that the Medaka fish transposon, Tol2, is able to stably integrate into the X. tropicalis genome and will serve as a powerful tool for insertional mutagenesis strategies in the frog...
  85. ncbi Targeted Sleeping Beauty transposition in human cells
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Mol Ther 15:1137-44. 2007
    ..Targeted transposition can be a powerful technology for safe transgene integration in human therapeutic applications...
  86. ncbi Functional characterization of IS1999, an IS4 family element involved in mobilization and expression of beta-lactam resistance genes
    Daniel Aubert
    Service de Bacteriologie Virologie, Hopital de Bicetre, 78 rue du General Leclerc, 94275 Le Kremlin Bicetre cedex, France
    J Bacteriol 188:6506-14. 2006
    ..This reduced transposition frequency of IS1999.2-based elements may result from a lower transcription of the transposase gene, as revealed by reverse transcription-PCR analyses...
  87. ncbi Positive selection on transposase genes of insertion sequences in the Crocosphaera watsonii genome
    Ted H M Mes
    Netherlands Institute of Ecology NIOO KNAW, Centre for Estuarine and Marine Ecology, POB 140, 4400 AC Yerseke, The Netherlands
    J Bacteriol 188:7176-85. 2006
    ..Intragenic adaptation of IS-carried genes identified here may constitute a novel mechanism associated with bacterial diversification and adaptation...
  88. ncbi Synapsis of Tn3 recombination sites: unpaired sites destabilize synapses by a partner exchange mechanism
    Jiuya He
    Institute of Biomedical and Life Sciences, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, UK
    J Mol Biol 319:385-93. 2002
    ..This mechanism reconciles studies on selective synapsis in multi-res substrates, which imply rapid interchange of synaptic pairings, with studies indicating that synapses of two Tn3res sites are stable...
  89. ncbi DNA-binding activity and subunit interaction of the mariner transposase
    L Zhang
    Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK
    Nucleic Acids Res 29:3566-75. 2001
    ..This mutant enzyme allows insertion at dinucleotides other than TA, including sequences with GC base pairs. This is the first example of a mariner/Tc1 transposase with altered target specificity...
  90. ncbi Rapid inactivation of the maize transposable element En/Spm in Medicago truncatula
    V Cosson
    , CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Mol Genet Genomics 269:732-45. 2003
    ..thaliana and even lower in M. truncatula. The use of different sources of transposases suggested that this defect in transposition was associated with the dSpm element itself...
  91. ncbi Transposition and gene disruption in the male germline of the mouse
    A J Dupuy
    Beckman Center for Transposon Research, University of Minnesota Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Genesis 30:82-8. 2001
    ..This technique has promise as a new strategy for forward genetic screens in the mouse or functional genomics...
  92. ncbi ISZm1068: an IS5-like insertion element from Zymomonas mobilis
    M Galeros
    Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Panepistemiopolis, Kouponia, Greece
    Arch Microbiol 175:323-33. 2001
    ..both at the nucleotide and at the amino acid sequence level with the corresponding ORFs encoding the transposases of many IS5 family elements, in particular the IS1031 group. Moreover, the Z...
  93. ncbi Determinants for hairpin formation in Tn10 transposition
    J S Allingham
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5B7
    EMBO J 20:2931-42. 2001
    ..In contrast, a mutation at another conserved position, W265, is shown to relax the specificity of the hairpin formation reaction...
  94. ncbi Genetic footprinting in bacteria
    R S Hare
    Schering Plough Research Institute, Kenilworth, New Jersey 07033, USA
    J Bacteriol 183:1694-706. 2001
    ..In addition, the ability of recombinant proteins to complement mutagenized hosts has been evaluated by genetic footprinting using a bacteriophage lambda transposon delivery system...
  95. ncbi Genomic characterization of Rim2/Hipa elements reveals a CACTA-like transposon superfamily with unique features in the rice genome
    G-D Wang
    SHARF and National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032 Shanghai, China
    Mol Genet Genomics 270:234-42. 2003
    ..analysis indicates that the putative RIM2 proteins fall into a subgroup distinct from the TNP2-like subgroup of transposases. Southern hybridization with genomic DNA from monocotyledonous and dicotyledonous plants demonstrates that the ..
  96. ncbi The Mu repressor-DNA complex contains an immobilized 'wing' within the minor groove
    J M Wojciak
    Department of Chemistry and Biochemistry, Molecular Biology Institute and the UCLA-DOE Laboratory of Structural Biology and Molecular Medicine, University of California, Los Angeles 90095, USA
    Nat Struct Biol 8:84-90. 2001
    ..Extensive contacts are also formed between the recognition helix and the DNA, which reads the major groove of a highly conserved region of the binding site through a single base-specific hydrogen bond and van der Waals contacts...
  97. ncbi Detection and analysis of transpositionally active head-to-tail dimers in three additional Escherichia coli IS elements
    Ildiko Szeverenyi
    Environmental Biosafety Research Institute, Agricultural Biotechnology Centre, Szent Györgyi Albert str 4, H 2101 Gödöllõ, Hungary
    Microbiology 149:1297-310. 2003
    ..The results presented for IS3, IS150 and IS186 are in accordance with the IS dimer model, which is in turn consistent with models based on covalently closed minicircles...
  98. ncbi The DNA-bending protein HMGB1 is a cellular cofactor of Sleeping Beauty transposition
    Hatem Zayed
    , , D-13092 Berlin, Germany
    Nucleic Acids Res 31:2313-22. 2003
    ....
  99. ncbi Transposase and cointegrase: specialized transposition proteins of the bacterial insertion sequence IS21 and related elements
    B Berger
    Laboratoire de Biologie Microbienne, , Switzerland
    Cell Mol Life Sci 58:403-19. 2001
    ..This review also includes a survey of the IS21 family and speculates on the possibility that other members present a similar transpositional specialization...
  100. ncbi Transposition of cyanobacterium insertion element ISY100 in Escherichia coli
    Akihiro Urasaki
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan
    J Bacteriol 184:5104-12. 2002
    ..ISY100 transposes in E. coli, indicating that it transposes without any host factor other than the transposase encoded by itself. Therefore, it may be able to transpose in other biological systems...
  101. ncbi Sin recombinase from Staphylococcus aureus: synaptic complex architecture and transposon targeting
    Sally J Rowland
    Institute of Biomedical and Life Sciences, University of Glasgow, Anderson College, UK
    Mol Microbiol 44:607-19. 2002
    ..Our model for the recombination synapse has at its core an assembly of four Sin dimers; Hbsu plays an architectural role that is taken by two resolvase dimers in models of the Tn3/gammadelta synapse...

Research Grants86

  1. BRUCELLA VACCINE FOR BIOTERRORISM
    Gary Splitter; Fiscal Year: 2004
    ..Included in these islands are ORFs encoding hypothetical proteins, transporters, transposases, and transcriptional regulators that most likely aid successful establishment of Brucella in human infections...
  2. GENE AMPLIFICATION BY A TRANSPOSITION APPARATUS
    Hiroshi Nakai; Fiscal Year: 1999
    ..the component that catalyzes strand transfer, performing a function similar to retroviral integrases and other transposases. However, the participation of the Mu A protein is not over after catalyzing strand transfer...
  3. Genetic transformation system for Chlamydia suis
    DANIEL ROCKEY; Fiscal Year: 2007
    ..the island encode plasmid mobilization and replication functions, tetracycline efflux pump, and candidate transposases. Experimental data in a surrogate system suggests that one of these transposases was responsible for the ..
  4. Plant Transposases and their MITEs
    Susan Wessler; Fiscal Year: 2007
    ..As such, knowledge of TE-mediated mechanisms of diversification are intimately involved in improving human health and understanding how plant and animal genomes evolve. ..
  5. A gene-trap screen for hearing and balance
    DONNA FEKETE; Fiscal Year: 2007
    ..Pseudotyped retroviral vectors or Tol2 transposases will be used to insert a gene-trap construct into the zebrafish germline...
  6. TRANSPOSASES IN ETOPOSIDE RESISTANCE
    Robert A Hromas; Fiscal Year: 2010
    ..In addition, the levels of Metnase could predict which patient will respond to etoposide. ..
  7. MECHANISTIC ANALYSIS OF DNA TRANSPOSITION BY PHAGE MU
    Tania Baker; Fiscal Year: 2001
    ..become clear that Mu transposase is a member of a structurally related family of proteins that includes many transposases and the retroviral integrases. These proteins catalyze recombination as multimeric complexes bound to DNA...
  8. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    ADRIENNE COTTRELL; Fiscal Year: 2007
    ..To ascertain if the transposases of the IS110/IS492 Family and Piv, the site-specific DNA recombinase, define a new family of DNA recombinases ..
  9. MUTATOR TRANSPORTABLE ELEMENTS OF MAIZE
    Virginia Walbot; Fiscal Year: 2005
    ..regulated timing of events and switch in transpositional outcome will be elucidated by analyzing proteins (MURA transposases and MURB helper proteins) encoded by the regulatory transposon MuDR...
  10. MOLECULAR ANALYSIS OF THE INSERTION ELEMENT IS903
    KEITH DERBYSHIRE; Fiscal Year: 2004
    ..In particular, mutant transposases will be sought that have altered binding specificity, increased transposition activity, and the ability to ..
  11. BACTERIOPHAGE MU DNA TRANSPOSITION
    Rasika Harshey; Fiscal Year: 1992
    ..We propose experiments that specifically address the post-transcriptional regulation of A- protein synthesis. We hope to uncover novel ways in which transposases limit their expression and thereby their mutagenic potential.
  12. Human DNA transposons: evolutionary history and genomic impact
    CEDRIC FESCHOTTE; Fiscal Year: 2007
    ..The second aim of this research is to assess the enzymatic activities of two transposases, MAR and PGBD3, which are potentially expressed in several human tissues and were identified as being among the ..
  13. Human DNA transposons: evolutionary history and genomic impact
    CEDRIC FESCHOTTE; Fiscal Year: 2010
    ..The second aim of this research is to assess the enzymatic activities of two transposases, MAR and PGBD3, which are potentially expressed in several human tissues and were identified as being among the ..
  14. MUTATOR TRANSPORTABLE ELEMENTS OF MAIZE
    Virginia Walbot; Fiscal Year: 2001
    ..In vitro biochemical studies will examine the DNA binding properties and enzymatic activities of the transposes encoded by MuDR to determine if different transposases are required for "cut and paste" and replicative transposition.
  15. Secretion systems for anti-malarial bacterial strains
    David Lampe; Fiscal Year: 2006
    ..agglomerans that secrete antimalarial proteins can block the transmission of malaria from mosquitoes to their hosts in a rodent malaria model system. Both Type I and Type II secretion systems will be developed and tested. ..
  16. Genetic methods using the Tol2 transposable element
    Koichi Kawakami; Fiscal Year: 2006
    ..abstract_text> ..
  17. Characterization of V(D)J cleavage and repair complexes
    PATRICK SWANSON; Fiscal Year: 2007
    ..abstract_text> ..
  18. Gustatory and olfactory receptors of Anopheles gambiae
    Hugh Robertson; Fiscal Year: 2006
    ..This work will also contribute to our overall understanding of the molecular basis of chemoreception in insects and other animals. ..
  19. MARINER TRANSPOSONS OF MAMMALS
    Hugh Robertson; Fiscal Year: 2001
    ..It will also reveal how occasionally particular copies of transposons can become integral parts of their host genetic complements. ..
  20. THE MOLECULAR BASIS OF HAEMOPHILUS DUCREYI PATHOGENESIS
    THOMAS KAWULA; Fiscal Year: 2001
    ..The role of the exported SOS in H. ducreyi survival and lesion development will be evaluated in the swine model of chancroid using both normal and neutropenic pigs. ..
  21. MOLECULAR ANALYSIS OF IS50/TN5 TRANSPOSITION
    William Reznikoff; Fiscal Year: 2003
    ..During the next grant period we will extend our genetic, biochemical and structural studies of Tn5 transposition. ..
  22. Structural Biology of a Serine DNA Recombinase
    PHOEBE RICE; Fiscal Year: 2007
    ..It will also help in the development of more versatile genetic tools. ..
  23. Molecular recognition studies of human PB1 bromodomains
    Martin Thompson; Fiscal Year: 2007
    ....
  24. Mechanisms of Immune Avoidance by Haemophilus ducreyi
    THOMAS KAWULA; Fiscal Year: 2007
    ..ducreyi re infection. An H. ducreyi outer membrane protein that is a target for bactericidal antibody has been identified. This protein will be tested for the ability to elicit a protective immune response to H. ducreyi. ..
  25. Understanding DNA specificity in the IHF/HU family
    PHOEBE RICE; Fiscal Year: 2005
    ..Hypotheses based on comparing and contrasting such data for the 3 different proteins to be studied will be tested by site-directed mutagenesis and domain-swap experiments. ..