transposases

Summary

Summary: Enzymes that recombine DNA segments by a process which involves the formation of a synapse between two DNA helices, the cleavage of single strands from each DNA helix and the ligation of a DNA strand from one DNA helix to the other. The resulting DNA structure is called a Holliday junction which can be resolved by DNA REPLICATION or by HOLLIDAY JUNCTION RESOLVASES.

Top Publications

  1. pmc piggyBac can bypass DNA synthesis during cut and paste transposition
    Rupak Mitra
    Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
    EMBO J 27:1097-109. 2008
  2. ncbi Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells
    Z Ivics
    Department of Genetics and Cell Biology, University of Minnesota, St Paul 55108 1095, USA
    Cell 91:501-10. 1997
  3. ncbi Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates
    Lajos Mátés
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Genet 41:753-61. 2009
  4. pmc Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Methods 6:363-9. 2009
  5. pmc Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery
    Melanie Galla
    Department of Experimental Hematology, Institute of Transplantation Immunology, Institute of Cell and Molecular Pathology, Hannover Medical School, Hannover, Germany
    Nucleic Acids Res 39:7147-60. 2011
  6. pmc Comparative genomic integration profiling of Sleeping Beauty transposons mobilized with high efficacy from integrase-defective lentiviral vectors in primary human cells
    Brian Moldt
    Department of Human Genetics, University of Aarhus, Aarhus, Denmark
    Mol Ther 19:1499-510. 2011
  7. pmc The catalytic domain of all eukaryotic cut-and-paste transposase superfamilies
    Yao Wu Yuan
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 108:7884-9. 2011
  8. pmc A hyperactive piggyBac transposase for mammalian applications
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 108:1531-6. 2011
  9. ncbi Disassembly of the Mu transposase tetramer by the ClpX chaperone
    I Levchenko
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139, USA
    Genes Dev 9:2399-408. 1995
  10. pmc Transposases are the most abundant, most ubiquitous genes in nature
    Ramy K Aziz
    Computation Institute, University of Chicago, Chicago, IL 60637, USA
    Nucleic Acids Res 38:4207-17. 2010

Detail Information

Publications269 found, 100 shown here

  1. pmc piggyBac can bypass DNA synthesis during cut and paste transposition
    Rupak Mitra
    Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
    EMBO J 27:1097-109. 2008
    ..This is the same strategy for target joining used by the members of DDE superfamily of transposases and retroviral integrases...
  2. ncbi Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells
    Z Ivics
    Department of Genetics and Cell Biology, University of Minnesota, St Paul 55108 1095, USA
    Cell 91:501-10. 1997
    ..Sleeping Beauty is an active DNA-transposon system from vertebrates for genetic transformation and insertional mutagenesis...
  3. ncbi Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates
    Lajos Mátés
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Genet 41:753-61. 2009
    ....
  4. pmc Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Methods 6:363-9. 2009
    ..iPSCs fulfilled all criteria of pluripotency, such as pluripotency gene expression, teratoma formation and contribution to chimeras. piggyBac transposon-based reprogramming may be used to generate therapeutically applicable iPSCs...
  5. pmc Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery
    Melanie Galla
    Department of Experimental Hematology, Institute of Transplantation Immunology, Institute of Cell and Molecular Pathology, Hannover Medical School, Hannover, Germany
    Nucleic Acids Res 39:7147-60. 2011
    ..The potential cytotoxicity of transposases requires careful assessment, considering that residual integration events of transposase expression vectors ..
  6. pmc Comparative genomic integration profiling of Sleeping Beauty transposons mobilized with high efficacy from integrase-defective lentiviral vectors in primary human cells
    Brian Moldt
    Department of Human Genetics, University of Aarhus, Aarhus, Denmark
    Mol Ther 19:1499-510. 2011
    ..Based on these properties, HIV-1/SB vectors may become valuable tools for genetic engineering and therapeutic gene transfer...
  7. pmc The catalytic domain of all eukaryotic cut-and-paste transposase superfamilies
    Yao Wu Yuan
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 108:7884-9. 2011
    ..g., hAT, P) based on sequence similarity of the element-encoded transposase. The transposases from several superfamilies possess a protein domain containing an acidic amino acid triad (DDE or DDD) that ..
  8. pmc A hyperactive piggyBac transposase for mammalian applications
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 108:1531-6. 2011
    ..This hyperactive piggyBac transposase expands the utility of the piggyBac transposon for applications in mammalian genetics and gene therapy...
  9. ncbi Disassembly of the Mu transposase tetramer by the ClpX chaperone
    I Levchenko
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139, USA
    Genes Dev 9:2399-408. 1995
    ..These data contribute to the emerging picture that members of the Clp family are chaperones specifically suited for disaggregating proteins and are able to function with or without a collaborating protease...
  10. pmc Transposases are the most abundant, most ubiquitous genes in nature
    Ramy K Aziz
    Computation Institute, University of Chicago, Chicago, IL 60637, USA
    Nucleic Acids Res 38:4207-17. 2010
    ..archaeal, eukaryotic and viral genomes and metagenomes, and our analysis demonstrates that genes encoding transposases are the most prevalent genes in nature...
  11. pmc The ancient mariner sails again: transposition of the human Hsmar1 element by a reconstructed transposase and activities of the SETMAR protein on transposon ends
    Csaba Miskey
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, Berlin, Germany
    Mol Cell Biol 27:4589-600. 2007
    ..The novel transposon system can be a useful tool for genome manipulations in vertebrates and for investigations into the transpositional dynamics and the contributions of these elements to primate genome evolution...
  12. pmc Molecular architecture of the Mos1 paired-end complex: the structural basis of DNA transposition in a eukaryote
    Julia M Richardson
    School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland
    Cell 138:1096-108. 2009
    ..Mixing experiments indicate that a transposase dimer initiates first-strand cleavage and suggest a pathway for PEC formation...
  13. pmc piggyBac is a flexible and highly active transposon as compared to sleeping beauty, Tol2, and Mos1 in mammalian cells
    Sareina Chiung Yuan Wu
    Cancer Research Center, Medical College of Georgia, Augusta, GA 30912, USA
    Proc Natl Acad Sci U S A 103:15008-13. 2006
    ..The high transposition activity of piggyBac and the flexibility for molecular modification of its transposase suggest the possibility of using it routinely for mammalian transgenesis...
  14. pmc Helper-independent piggyBac plasmids for gene delivery approaches: strategies for avoiding potential genotoxic effects
    Johann Urschitz
    Department of Anatomy, John A Burns School of Medicine, Honolulu, HI 96822, USA
    Proc Natl Acad Sci U S A 107:8117-22. 2010
    ....
  15. ncbi The Sleeping Beauty transposon toolbox
    Ismahen Ammar
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Methods Mol Biol 859:229-40. 2012
    ..This review highlights the state-of-the-art in SB transposon technology in diverse genetic applications with special emphasis on the transposon as well as transposase vectors currently available in the SB transposon toolbox...
  16. pmc Retargeting transposon insertions by the adeno-associated virus Rep protein
    Ismahen Ammar
    Max Delbruck Center for Molecular Medicine, 13125 Berlin, Germany
    Nucleic Acids Res 40:6693-712. 2012
    ..A series of fusion constructs consisting of the N-terminal DNA-binding domain of Rep and the transposases or the N57 domain of SB were generated...
  17. pmc Integrating prokaryotes and eukaryotes: DNA transposases in light of structure
    Alison Burgess Hickman
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD 20892, USA
    Crit Rev Biochem Mol Biol 45:50-69. 2010
    ..DNA transposases comprise one class of such enzymes...
  18. pmc Transposition of the human Hsmar1 transposon: rate-limiting steps and the importance of the flanking TA dinucleotide in second strand cleavage
    Corentin Claeys Bouuaert
    School of Biomedical Sciences, University of Nottingham, Queen s Medical Centre, Nottingham, UK
    Nucleic Acids Res 38:190-202. 2010
    ..Furthermore, this conformational change requires specific contacts between transposase and the flanking TA dinucleotide...
  19. pmc Genome-wide analysis of mariner-like transposable elements in rice reveals complex relationships with stowaway miniature inverted repeat transposable elements (MITEs)
    CEDRIC FESCHOTTE
    Department of Plant Biology, The University of Georgia, Athens, Georgia 30602, USA
    Genetics 163:747-58. 2003
    ..These data suggest a model whereby most of the Stowaway MITEs in rice were cross-mobilized by MLE transposases encoded by distantly related elements.
  20. pmc ClpX protein of Escherichia coli activates bacteriophage Mu transposase in the strand transfer complex for initiation of Mu DNA synthesis
    R Kruklitis
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    EMBO J 15:935-44. 1996
    ..These results indicate that ClpX protein activates MuA in STC1 so that it can recruit crucial host factors needed to initiate Mu DNA synthesis by specific replication enzymes...
  21. pmc Generation of an inducible and optimized piggyBac transposon system
    Juan Cadinanos
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
    Nucleic Acids Res 35:e87. 2007
    ..As the activity of PB is higher than that of SB11 and SB12, two hyperactive SB transposases, we have characterized and improved the PB system in mouse ES cells...
  22. ncbi Development of hyperactive sleeping beauty transposon vectors by mutational analysis
    Hatem Zayed
    Max Delbruck Center for Molecular Medicine, Robert Rössle Strasse10, D 13092 Berlin, Germany
    Mol Ther 9:292-304. 2004
    ..transposons and transiently overexpressed HMGB1, a cellular cofactor of SB transposition, hyperactive transposases elevate transposition by almost an order of magnitude compared to the first-generation transposon system...
  23. ncbi Resident aliens: the Tc1/mariner superfamily of transposable elements
    R H Plasterk
    Division of Molecular Biology, Netherlands Cancer Institute and Center for Biomedical Genetics, Division of Molecular Biology, Plesmanlaan 121, Amsterdam 1066CX, The Netherlands
    Trends Genet 15:326-32. 1999
    ....
  24. pmc A transposon and transposase system for human application
    Perry B Hackett
    Department of Genetics, Cell Biology, and Development, Center for Genome Engineering, Institute of Human Genetics, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 18:674-83. 2010
    ..This review examines the rationale and safety implications of application of the SB system to genetically modify T cells to be manufactured in compliance with current good manufacturing practice (cGMP) for phase I/II trials...
  25. pmc A simple topological filter in a eukaryotic transposon as a mechanism to suppress genome instability
    Corentin Claeys Bouuaert
    School of Biomedical Sciences, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2UH, United Kingdom
    Mol Cell Biol 31:317-27. 2011
    ..This provides constraints on the enzymatic mechanism of the double-strand breaks in mariner transposition, excluding the most prevalent of the current models...
  26. pmc Solution conformations of early intermediates in Mos1 transposition
    Maxime G Cuypers
    Life Sciences Group, Institut Laue Langevin ILL, 6 rue Jules Horowitz, 38042 Grenoble, France
    Nucleic Acids Res 41:2020-33. 2013
    DNA transposases facilitate genome rearrangements by moving DNA transposons around and between genomes by a cut-and-paste mechanism...
  27. pmc Targeted engineering of the Caenorhabditis elegans genome following Mos1-triggered chromosomal breaks
    Valerie Robert
    ENS, Biologie Cellulaire de la Synapse, Paris, France INSERM, U789, Paris, France
    EMBO J 26:170-83. 2007
    ..In conjunction with a publicly available Mos1 insertion library currently being generated, MosTIC will provide a general tool to customize the C. elegans genome...
  28. pmc Hyperactive self-inactivating piggyBac for transposase-enhanced pronuclear microinjection transgenesis
    Joel Marh
    Department of Anatomy, John A Burns School of Medicine, University of Hawaii, Honolulu, HI 96822, USA
    Proc Natl Acad Sci U S A 109:19184-9. 2012
    ..This transposase-mediated approach is also very efficient for single-cell embryo cytoplasmic injections, offering an easy-to-implement transgenesis method to the scientific community...
  29. ncbi Gene transfer into genomes of human cells by the sleeping beauty transposon system
    Aron M Geurts
    Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Mol Ther 8:108-17. 2003
    ..With the recently described improvements to the transposon itself, the SB system appears to be a potential gene-transfer tool for human gene therapy...
  30. pmc Identification and characterization of repetitive extragenic palindromes (REP)-associated tyrosine transposases: implications for REP evolution and dynamics in bacterial genomes
    Jaroslav Nunvar
    Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic
    BMC Genomics 11:44. 2010
    ..BIMEs and REPs have been shown to serve as binding sites for several proteins and suggested to play role in chromosome organization and transcription termination. Their origins are, at present, unknown...
  31. pmc Manipulating piggyBac transposon chromosomal integration site selection in human cells
    Claudia Kettlun
    Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Ther 19:1636-44. 2011
    ..Our results offer insight into the requirements for using a chimeric zinc finger-piggyBac transposase to direct integration into a user-defined chromosomal location...
  32. pmc The AAA+ ClpX machine unfolds a keystone subunit to remodel the Mu transpososome
    Aliaa H Abdelhakim
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 107:2437-42. 2010
    ..The specific targeting of a stabilizing "keystone subunit" within a complex for unfolding is an attractive general mechanism for remodeling by AAA+ enzymes...
  33. pmc Redirecting specificity of T-cell populations for CD19 using the Sleeping Beauty system
    Harjeet Singh
    Division of Pediatrics, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Cancer Res 68:2961-71. 2008
    ..When coupled with numerical expansion on CD19(+) artificial antigen-presenting cells, this gene transfer method results in rapid outgrowth of CD4(+) and CD8(+) T cells expressing CAR to redirect specificity for CD19(+) tumor cells...
  34. pmc Gene transfer efficiency and genome-wide integration profiling of Sleeping Beauty, Tol2, and piggyBac transposons in human primary T cells
    Xin Huang
    Division of Pediatric Blood and Marrow Transplantation, University of Minnesota Medical School, Minneapolis, Minnesota, USA
    Mol Ther 18:1803-13. 2010
    ....
  35. ncbi RNA as a source of transposase for Sleeping Beauty-mediated gene insertion and expression in somatic cells and tissues
    Andrew Wilber
    The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 13:625-30. 2006
    ..We conclude that in vitro-transcribed mRNA can be used as an effective source of transposase for SB-mediated transposition in mammalian cells and tissues...
  36. ncbi Delivering the goods: viral and non-viral gene therapy systems and the inherent limits on cargo DNA and internal sequences
    Helen Atkinson
    School of Biomedical Sciences, University of Nottingham, Queen s Medical Center, Nottingham NG7 2UH, UK
    Genetica 138:485-98. 2010
    ..Length-dependence is a complex phenomenon driven not simply by the distance between the transposon ends, but by host proteins, the transposase and the properties of the DNA sequences encoded within the transposon...
  37. ncbi Excision of the Drosophila mariner transposon Mos1. Comparison with bacterial transposition and V(D)J recombination
    Angela Dawson
    Institute of Cell and Molecular Biology, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JR, United Kingdom
    Mol Cell 11:225-35. 2003
    ..Unlike V(D)J recombination, however, second strand cleavage of mariner does not occur via a hairpin intermediate...
  38. pmc Trans-kingdom transposition of the maize dissociation element
    Alexander Emelyanov
    Temasek Life Sciences Laboratory, The National University of Singapore, 117604 Singapore, Singapore
    Genetics 174:1095-104. 2006
    ....
  39. pmc Site-directed transposon integration in human cells
    Stephen R Yant
    Department of Pediatrics and Genetics, Stanford University School of Medicine, Stanford, CA 94305 5208, USA
    Nucleic Acids Res 35:e50. 2007
    ..These findings illustrate the feasibility of directed transposon integration and highlight potential means for future development...
  40. pmc Duration of expression and activity of Sleeping Beauty transposase in mouse liver following hydrodynamic DNA delivery
    Jason B Bell
    Department of Genetics, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 18:1796-802. 2010
    ..Thus, within the limits of current technology, we show that SB transposons appear to be as stably integrated as their viral counterparts...
  41. ncbi Using rice to understand the origin and amplification of miniature inverted repeat transposable elements (MITEs)
    Ning Jiang
    Department of Plant Biology, University of Georgia, Athens, Georgia 30602, USA
    Curr Opin Plant Biol 7:115-9. 2004
    ..provided answers to many of the outstanding questions regarding the existence of active MITEs, their source of transposases (TPases) and their chromosomal distribution...
  42. ncbi Autoregulation of mariner transposase activity by overproduction and dominant-negative complementation
    A R Lohe
    Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Mol Biol Evol 13:549-55. 1996
    ..Dominant-negative complementation may help explain why most naturally occurring copies of MLEs have been mutationally inactivated...
  43. ncbi Involvement of a bifunctional, paired-like DNA-binding domain and a transpositional enhancer in Sleeping Beauty transposition
    Zsuzsanna Izsvák
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, D 13092 Berlin, Germany
    J Biol Chem 277:34581-8. 2002
    ..These interactions may result in enhanced stability of synaptic complexes, which might explain the efficient transposition of Sleeping Beauty in vertebrate cells...
  44. pmc Convergent domestication of pogo-like transposases into centromere-binding proteins in fission yeast and mammals
    Claudio Casola
    Department of Biology, University of Texas, Arlington, USA
    Mol Biol Evol 25:29-41. 2008
    ..Each of these proteins displays extensive sequence similarity with pogo-like transposases, which have been previously identified in the genomes of various insects and vertebrates, in the protozoan ..
  45. pmc Unexpectedly high copy number of random integration but low frequency of persistent expression of the Sleeping Beauty transposase after trans delivery in primary human T cells
    Xin Huang
    Pediatric Blood and Marrow Transplantation, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Hum Gene Ther 21:1577-90. 2010
    ..Our data suggest that SB delivery via plasmid in T cells should be carried out with caution because of unexpectedly high copy numbers of randomly integrated SB transposase...
  46. pmc A functional role for transposases in a large eukaryotic genome
    Mariusz Nowacki
    Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA
    Science 324:935-8. 2009
    ..transposase genes play key roles in this process of genome-wide DNA excision, which suggests that transposases function in large eukaryotic genomes containing thousands of active transposons...
  47. pmc DNA-binding activity and subunit interaction of the mariner transposase
    L Zhang
    Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK
    Nucleic Acids Res 29:3566-75. 2001
    ..This mutant enzyme allows insertion at dinucleotides other than TA, including sequences with GC base pairs. This is the first example of a mariner/Tc1 transposase with altered target specificity...
  48. ncbi The bacterial Tn9 chloramphenicol resistance gene: an attractive DNA segment for Mos1 mariner insertions
    Gwénaelle Crénès
    GICC, UMR CNRS 6239, Universite Francois Rabelais de Tours, UFR des Sciences et Techniques, Parc de Grandmont, 37200, Tours, France
    Mol Genet Genomics 281:315-28. 2009
    ..All TA dinucleotides that are preferred for integration were found within either TATA or TA x TA motifs. However, these motifs are not sufficient to constitute an attractive dinucleotide, since four TATA and TA x TA sites are cold spots...
  49. pmc Comparative analysis of the recently discovered hAT transposon TcBuster in human cells
    Lauren E Woodard
    Department of Medicine, Division of Nephrology, Baylor College of Medicine, Houston, Texas, USA
    PLoS ONE 7:e42666. 2012
    ..TcBuster, a novel hAT-family transposon system derived from the red flour beetle Tribolium castaneum, was shown to be highly active in previous studies in insect embryoes...
  50. ncbi Transposon Tn5
    William S Reznikoff
    Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Woods Hole, Massachusetts 02543, USA
    Annu Rev Genet 42:269-86. 2008
    ....
  51. ncbi Transposition from a gutless adeno-transposon vector stabilizes transgene expression in vivo
    Stephen R Yant
    Department of Pediatrics and Genetics, Stanford University School of Medicine, Stanford, CA 94305 5208, USA
    Nat Biotechnol 20:999-1005. 2002
    ..These vectors combine the versatility of adenoviral vectors with the integration capabilities of a eukaryotic DNA transposon and should prove useful in the treatment of genetic diseases...
  52. ncbi Target site selection by the mariner-like element, Mos1
    Gwénaelle Crénès
    Universite Francois Rabelais de Tours, GICC, Parc de Grandmont, 37200 Tours, France
    Genetica 138:509-17. 2010
    ..Analysis of the physical properties of sequences obtained in vitro and in vivo do not help to explain Mos1 integration preferences, suggesting that other characteristics must be involved in Mos1 target choice...
  53. ncbi Plant transposable elements: where genetics meets genomics
    CEDRIC FESCHOTTE
    Departments of Plant Biology and Genetics, The University of Georgia, Athens, Georgia 30602, USA
    Nat Rev Genet 3:329-41. 2002
    ..Nowhere is this shift more evident than in plants, in which transposable elements were first discovered and where they are still actively reshaping genomes...
  54. ncbi Bacterial genetic methods to explore the biology of mariner transposons
    David J Lampe
    Department of Biological Sciences, Duquesne University, 600 Forbes Ave, Pittsburgh, PA 15116, USA
    Genetica 138:499-508. 2010
    ..Finally, it is possible to uncover functional transposase genes directly from genomes given a suitable bacterial genetic screen...
  55. ncbi Crystal structure of the human Hsmar1-derived transposase domain in the DNA repair enzyme Metnase
    Kristie D Goodwin
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Biochemistry 49:5705-13. 2010
    ....
  56. pmc A genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community
    Elodie Vallin
    Centre de Génétique et de Physiologie Moléculaires et Cellulaires, CNRS UMR 5534, Campus de la Doua, Villeurbanne, France
    PLoS ONE 7:e30482. 2012
    ..elegans genes and the modification of more than 40% of them. This collection of mutants, generated under the auspices of the European NEMAGENETAG consortium, is publicly available and represents an important research resource...
  57. ncbi Sleeping beauty--a mouse model for all cancers?
    Viive M Howell
    Functional Genomics Laboratory, Kolling Institute of Medical Research, University of Sydney, E25, Level 9, Kolling Building, Royal North Shore Hospital, St Leonards, NSW 2065, Australia
    Cancer Lett 317:1-8. 2012
    ..This review follows the refinement of SB for different cancers and assesses its potential as a model for all cancers and a tool for cancer gene discovery...
  58. pmc Sleeping Beauty transposon-based system for cellular reprogramming and targeted gene insertion in induced pluripotent stem cells
    Ivana Grabundzija
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13125 Berlin, Germany
    Nucleic Acids Res 41:1829-47. 2013
    ..This strategy would allow correction of a genetic defect by site-specific insertion of a therapeutic gene construct into 'safe harbor' sites in the genomes of autologous, patient-derived iPS cells...
  59. pmc Insertional mutagenesis by a hybrid piggyBac and sleeping beauty transposon in the rat
    Kenryo Furushima
    Department of Genetics, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Genetics 192:1235-48. 2012
    ..This mutagenesis system based on simple crosses and visual genotyping can be used to generate a collection of single-gene mutations in the rat...
  60. pmc A resurrected mammalian hAT transposable element and a closely related insect element are highly active in human cell culture
    Xianghong Li
    The Howard Hughes Medical Institute and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 110:E478-87. 2013
    ..Finally, we describe a Saccharomyces cerevisiae assay for TcBuster that will provide a means for isolation of hyperactive and other interesting classes of transposase mutants...
  61. pmc The SLEEPER genes: a transposase-derived angiosperm-specific gene family
    Marijn Knip
    Department of Molecular and Developmental Genetics, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE, Leiden, The Netherlands
    BMC Plant Biol 12:192. 2012
    ..We studied the presence of DAYSLEEPER orthologs in plants and propose a model for the domestication of the ancestral DAYSLEEPER gene in angiosperms...
  62. pmc Mechanism of IS200/IS605 family DNA transposases: activation and transposon-directed target site selection
    Orsolya Barabas
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 132:208-20. 2008
    The smallest known DNA transposases are those from the IS200/IS605 family. Here we show how the interplay of protein and DNA activates TnpA, the Helicobacter pylori IS608 transposase, for catalysis...
  63. pmc PiggyBac transposon-mediated, reversible gene transfer in human embryonic stem cells
    You Tzung Chen
    Graduate Institute of Clinical Genomics and Graduate Institute of Clinical Medicine, Stem Cell Core Laboratory, NTU Research Center for Medical Excellence, Taipei, Taiwan
    Stem Cells Dev 19:763-71. 2010
    ..We also demonstrate that the integrated piggyBac transposon can be removed and an undisrupted insertion site can be restored, which implies potential applications for its use in gene therapy and genetics studies...
  64. pmc Structuring the bacterial genome: Y1-transposases associated with REP-BIME sequences
    Bao Ton-Hoang
    Laboratoire de Microbiologie et Genetique Moleculaires, Centre National de la Recherche Scientifique, 118, route de Narbonne, 31062 Toulouse Cedex, France
    Nucleic Acids Res 40:3596-609. 2012
    ..also resemble the sub-terminal hairpins of the atypical IS200/605 family of insertion sequences which encode Y1 transposases (TnpA(IS200/IS605))...
  65. ncbi Target DNA bending is an important specificity determinant in target site selection in Tn10 transposition
    Patrick A Pribil
    Department of Biochemistry, University of Western Ontario, N6A 5B7, London, Ont, Canada
    J Mol Biol 330:247-59. 2003
    ..We also show that Ca(2+) facilitates target capture by stabilizing transposase interactions with sequences immediately flanking the insertion site. Ca(2+) could, in theory, exert this effect by stabilizing bends in the target DNA...
  66. pmc Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition
    Andrew Adey
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Genome Biol 11:R119. 2010
    ..We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing...
  67. ncbi Messenger RNA as a source of transposase for sleeping beauty transposon-mediated correction of hereditary tyrosinemia type I
    Andrew Wilber
    The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 15:1280-7. 2007
    ....
  68. ncbi Stable integration and conditional expression of electroporated transgenes in chicken embryos
    Yuki Sato
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara, 630 0192, Japan
    Dev Biol 305:616-24. 2007
    ..Thus, the techniques proposed in this study provide a novel approach to study the mechanisms of late organogenesis, for which chickens are most suitable model animals...
  69. pmc Prolonged expression of a lysosomal enzyme in mouse liver after Sleeping Beauty transposon-mediated gene delivery: implications for non-viral gene therapy of mucopolysaccharidoses
    Elena L Aronovich
    Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    J Gene Med 9:403-15. 2007
    ..The Sleeping Beauty (SB) transposon system is a non-viral vector system that can integrate precise sequences into chromosomes. We evaluated the SB transposon system as a tool for gene therapy of mucopolysaccharidosis (MPS) types I and VII...
  70. pmc Hyperactive piggyBac gene transfer in human cells and in vivo
    Joseph E Doherty
    Medical Scientist Training Program, Baylor College of Medicine, Houston, TX 77030, USA
    Hum Gene Ther 23:311-20. 2012
    ..We conclude that hyperactive piggyBac elements can increase gene transfer in human cells and in vivo and should enable improved gene delivery using the piggyBac transposon system in a variety of cell and gene-therapy applications...
  71. pmc Mechanism of Mos1 transposition: insights from structural analysis
    Julia M Richardson
    School of Biological Sciences, University of Edinburgh, Edinburgh, UK
    EMBO J 25:1324-34. 2006
    ..the crystal structure of the catalytic domain of Mos1 transposase, a member of the Tc1/mariner family of transposases. The structure comprises an RNase H-like core, bringing together an aspartic acid triad to form the active site,..
  72. pmc Sleeping beauty transposition from nonintegrating lentivirus
    Conrad A Vink
    Institute of Child Health, University College London, UK
    Mol Ther 17:1197-204. 2009
    ..Importantly, integration site analysis revealed redirection toward a profile mimicking SB-plasmid integration and away from integration within transcriptionally active genes favored by integrase-proficient lentiviral vectors (ILVs)...
  73. ncbi Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transfer
    Andrew Wilber
    The Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Stem Cells 25:2919-27. 2007
    ..We conclude that the SB transposon system provides an effective approach with several advantages for genetic manipulation and durable gene expression in human ES cells...
  74. ncbi Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli
    M Ariyoshi
    Protein Engineering Research Institute, Osaka, Japan
    Cell 78:1063-72. 1994
    ..The folding topology in the vicinity of the catalytic site exhibits a striking similarity to that of RNAase H1 from E. coli...
  75. pmc Characterization of the maize Mutator transposable element MURA transposase as a DNA-binding protein
    M I Benito
    Department of Biological Sciences, Stanford University, California 94305 5020, USA
    Mol Cell Biol 17:5165-75. 1997
    ..The significance of MURA's interaction with the TIRs of Mu elements is discussed in the context of what is known about the regulation and mechanisms of Mutator activities in maize...
  76. pmc Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition
    Akihiro Urasaki
    Division of Molecular and Developmental Biology, National Institute of Genetics, Japan
    Genetics 174:639-49. 2006
    ..Also, our study established a basis for further biochemical and molecular biological studies for understanding roles of the repetitive sequence in the subterminal region in transposition...
  77. ncbi Pig transgenesis by Sleeping Beauty DNA transposition
    Jannik E Jakobsen
    Department of Human Genetics, University of Aarhus, Wilh Meyers Allé 1240, 8000, Aarhus C, Denmark
    Transgenic Res 20:533-45. 2011
    ....
  78. pmc A novel DNA binding and nuclease activity in domain III of Mu transposase: evidence for a catalytic region involved in donor cleavage
    Z Wu
    Department of Biochemistry, University of Western Ontario, London, Canada
    EMBO J 14:3835-43. 1995
    ..This proposal for active site assembly is in agreement with the recently proposed domain sharing model by Yang et al. (Yang, J.Y., Kim, K., Jayaram, M. and Harshey, R.M. [1995] EMBO J., 14, 2374-2384)...
  79. pmc DNA repair by the cryptic endonuclease activity of Mu transposase
    Wonyoung Choi
    Section of Molecular Genetics and Microbiology and Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    Proc Natl Acad Sci U S A 107:10014-9. 2010
    ..We hypothesize that ClpX constitutes part of a highly regulated mechanism that unmasks the cryptic nuclease activity of MuA specifically in the repair pathway...
  80. ncbi Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system
    Adam J Dupuy
    National Cancer Institute, Center for Cancer Research, Frederick, Maryland 21702, USA
    Nature 436:221-6. 2005
    ..The uses of SB are also not restricted to the mouse and could potentially be used for forward genetic screens in any higher eukaryote in which transgenesis is possible...
  81. ncbi Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line
    J L Bessereau
    Department of Biology, University of Utah, Salt Lake City 84112-0840, USA
    Nature 413:70-4. 2001
    ..Fourth, these insertions can subsequently be remobilized to generate deletion and frameshift mutations by imperfect excision...
  82. pmc Efficient nonviral Sleeping Beauty transposon-based TCR gene transfer to peripheral blood lymphocytes confers antigen-specific antitumor reactivity
    P D Peng
    Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Gene Ther 16:1042-9. 2009
    ..These results demonstrate the promise of nonviral ex vivo genetic modification of autologous lymphocytes for the treatment of cancer and immunologic disease...
  83. pmc Chromosomal transposition of PiggyBac in mouse embryonic stem cells
    Wei Wang
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 105:9290-5. 2008
    ..The comprehensive information from this study should facilitate further exploration of the potential of PB and SB DNA transposons in mammalian genetics...
  84. ncbi Sustained FVIII expression and phenotypic correction of hemophilia A in neonatal mice using an endothelial-targeted sleeping beauty transposon
    Li Liu
    Department of Pharmacology and Therapeutics, College of Medicine, University of Florida, Gainesville, FL 32610, USA
    Mol Ther 13:1006-15. 2006
    ..These studies demonstrate the feasibility of using endothelial-targeted SB transposons for the treatment of hemophilia A...
  85. ncbi An Arabidopsis hAT-like transposase is essential for plant development
    Paul Bundock
    Institute for Biology, Leiden University, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands
    Nature 436:282-4. 2005
    ..for normal plant growth; it shares several characteristics with the hAT (hobo, Activator, Tam3) family of transposases. DAYSLEEPER was isolated as a factor binding to a motif (Kubox1) present in the upstream region of the ..
  86. pmc DNA recognition and the precleavage state during single-stranded DNA transposition in D. radiodurans
    Alison Burgess Hickman
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    EMBO J 29:3840-52. 2010
    Bacterial insertion sequences (ISs) from the IS200/IS605 family encode the smallest known DNA transposases and mobilize through single-stranded DNA transposition...
  87. pmc Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps
    Jochen Trauner
    1Department of Biology, Developmental Biology, Friedrich Alexander University Erlangen, Erlangen, Germany
    BMC Biol 7:73. 2009
    ..Even so, there is a pressing need for forward genetic analysis to escape the bias inherent in candidate gene approaches...
  88. pmc The processing of repetitive extragenic palindromes: the structure of a repetitive extragenic palindrome bound to its associated nuclease
    Simon A J Messing
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Nucleic Acids Res 40:9964-79. 2012
    ..Although a new clade of putative transposases (RAYTs or TnpA(REP)) is often associated with an increase in these repeats, it is not clear how these proteins ..
  89. ncbi Four genes, two ends, and a res region are involved in transposition of Tn5053: a paradigm for a novel family of transposons carrying either a mer operon or an integron
    G Y Kholodii
    Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia
    Mol Microbiol 17:1189-200. 1995
    ..We suggest that the tni genes were involved in the dissemination of integrons...
  90. ncbi ClpX and MuB interact with overlapping regions of Mu transposase: implications for control of the transposition pathway
    I Levchenko
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Genes Dev 11:1561-72. 1997
    ....
  91. ncbi Automatic classification within families of transposable elements: application to the mariner Family
    Jacques Deric Rouault
    Laboratoire Evolution, Genomes et Speciation, UPR9034, CNRS, 91198 Gif sur Yvette, France and Université Paris Sud 11, 91405 Orsay, France
    Gene 448:227-32. 2009
    ..The classification obtained here from DNA sequences of 935 items matches the phylogenies of the transposase. The rate of error from a posteriori re-assignment is relatively low...
  92. ncbi Recent horizontal transfer of mellifera subfamily mariner transposons into insect lineages representing four different orders shows that selection acts only during horizontal transfer
    David J Lampe
    Department of Biological Sciences, Duquesne University
    Mol Biol Evol 20:554-62. 2003
    ....
  93. pmc DDE transposases: Structural similarity and diversity
    Irina V Nesmelova
    Department of Physics and Optical Science, University of North Carolina, Charlotte, 28223, United States
    Adv Drug Deliv Rev 62:1187-95. 2010
    ..This process requires a transposase enzyme. During transposition DD[E/D]-transposases undergo a series of conformational changes...
  94. ncbi Duplication of cyb-3 (cyclin B3) suppresses sterility in the absence of mdf-1/MAD1 spindle assembly checkpoint component in Caenorhabditis elegans
    Maja Tarailo-Graovac
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada
    Cell Cycle 9:4858-65. 2010
    ..Importantly, we show here, for the first time, that doubling the CYB-3 (Cyclin B3) dosage suppresses sterility in the absence of the essential spindle assembly checkpoint component MDF-1 without causing a delay in the onset of anaphase...
  95. pmc Phylogenetic and functional characterization of the hAT transposon superfamily
    Peter Arensburger
    Department of Entomology, University of California, Riverside, CA 92521 0001, USA
    Genetics 188:45-57. 2011
    ..We find that the recently discovered SPIN transposons of mammals are located within the family of Buster elements...
  96. ncbi Cotranslational control of DNA transposition: a window of opportunity
    Guy Duval-Valentin
    Laboratoire de Microbiologie et Genetique Moleculaires, CNRS UMR5100, Campus Université Paul Sabatier, 118 route de Narbonne, F31062 Toulouse Cedex, France
    Mol Cell 44:989-96. 2011
    ..One IS regulatory phenomenon is a preference of their transposases (Tpases) for action on the element from which they are expressed (cis) rather than on other copies of the same ..
  97. ncbi Transposition of hAT elements links transposable elements and V(D)J recombination
    Liqin Zhou
    Howard Hughes Medical Institute and Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    Nature 432:995-1001. 2004
    ..Transposable elements are classified by their transposition mechanisms and by the transposases that mediate their movement...
  98. pmc Reconstitution of a functional IS608 single-strand transpososome: role of non-canonical base pairing
    Susu He
    Laboratoire de Microbiologie et Genetique Moleculaires, Centre National de Recherche Scientifique, Unité Mixte de Recherche 5100, 118 Rte de Narbonne, F31062 Toulouse Cedex, France
    Nucleic Acids Res 39:8503-12. 2011
    ....
  99. pmc Targeted gene deletions in C. elegans using transposon excision
    Christian Frøkjaer-Jensen
    Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City, Utah, USA
    Nat Methods 7:451-3. 2010
    ..Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker...
  100. ncbi Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex
    S D Fugmann
    Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Mol Cell 5:97-107. 2000
    ..we have identified a region of RAG1 with possible structural similarities to the active site regions of transposases and retroviral integrases...
  101. pmc A conditional transposon-based insertional mutagenesis screen for genes associated with mouse hepatocellular carcinoma
    Vincent W Keng
    Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Nat Biotechnol 27:264-74. 2009
    ....

Research Grants62

  1. Creation of hyperactive transposons for mutagenesis in rodents
    Eric M Ostertag; Fiscal Year: 2011
    ..In Phase I studies, we focused on synthesizing hyperactive transposases, from three different families of transposons, Sleeping Beauty (SB), piggyBac (PB), and TcBuster (TcB), to ..
  2. Improved bacterial strains for therapeutic DNA and protein production
    Frederick R Blattner; Fiscal Year: 2010
    ..stability and have eliminated many undesired fermentation side products including phage, flagellae, fimbrae, transposases and in some cases, toxins. Phase I of this project dealt with the problem of limiting the most serious E...
  3. Targeting Replication-Segregation of plasmid pX01 in Bacillus anthracis
    Debabrata RayChaudhuri; Fiscal Year: 2010
    ..large pathogenicity island that houses genes encoding the tripartite anthrax toxin and several intregrases and transposases. The presence of mobile elements in pXO1 allows gene transfer to related Bacillus cereus family members...
  4. Cancer gene discovery using Sleeping Beauty transposon-mediated mutagenesis
    TIMOTHY KAEHLER STARR; Fiscal Year: 2010
    ..Aim 3 proposes several improvements to the SB system, using newly developed highly active transposases and transposons, to uncover novel lung cancer genes...
  5. Cancer gene discovery using Sleeping Beauty transposon-mediated mutagenesis
    TIMOTHY KAEHLER STARR; Fiscal Year: 2013
    ..Aim 3 proposes several improvements to the SB system, using newly developed highly active transposases and transposons, to uncover novel lung cancer genes...
  6. Transposon Based Mammalian Transgenesis and Transfection
    Stefan Moisyadi; Fiscal Year: 2012
    ..Among approaches with protein recombinases and transposases, the hyperactive Tn5 transposase protein (*Tn5p) was by far the most efficient method when introducing the ..
  7. TRANSPOSASES IN ETOPOSIDE RESISTANCE
    Robert A Hromas; Fiscal Year: 2012
    ..that regulate the ability of Metnase to reduce etoposide DSBs? 2) What is the downstream pathway by which Metnase reduces etoposide DSBs? 3) Do Metnase levels predic clinical resistance to etoposide in human malignancy? ..
  8. Programmed DNA rearrangements in the ciliate Oxytricha trifallax
    BRIAN PATRICK HIGGINS; Fiscal Year: 2010
    ..Hypothesis 1: Conventional (non-scrambled) lESs are removed via a mechanism similar to that used by DDB transposases. Preliminary results show that a conventional IBScan be circularized upon removal...
  9. E. faecalis Pathogenicity Island
    Michael S Gilmore; Fiscal Year: 2013
    ..faecalis PAI evolved by the accretion of modules, possibly mediated by transposases or other site specific recombinases found in the PAI...
  10. Structural understanding of Mu transposition
    Phoebe A Rice; Fiscal Year: 2013
    ..This is the most thoroughly characterized of the large and highly diverse "DDE" family of transposases and retroviral integrases, and studies of Mu will continue to illuminate paradigms that translate to the rest ..
  11. Crystallographic Studies of Retroviral Integrases
    Hideki Aihara; Fiscal Year: 2010
    ..IN complex? How are the reactions at two viral DNA ends coordinated? How are retroviral INs different from DNA transposases that can perform both integration and excision? Despite the high medical relevance of retroviral IN, no crystal ..
  12. Crystallographic Studies of Retroviral Integrases
    Hideki Aihara; Fiscal Year: 2013
    ..IN complex? How are the reactions at two viral DNA ends coordinated? How are retroviral INs different from DNA transposases that can perform both integration and excision? Despite the high medical relevance of retroviral IN, no crystal ..
  13. BRUCELLA VACCINE FOR BIOTERRORISM
    Gary Splitter; Fiscal Year: 2004
    ..Included in these islands are ORFs encoding hypothetical proteins, transporters, transposases, and transcriptional regulators that most likely aid successful establishment of Brucella in human infections...
  14. Genetic transformation system for Chlamydia suis
    DANIEL ROCKEY; Fiscal Year: 2007
    ..the island encode plasmid mobilization and replication functions, tetracycline efflux pump, and candidate transposases. Experimental data in a surrogate system suggests that one of these transposases was responsible for the ..
  15. FACTORS REGULATING TRANSPOSITION IN C. ELEGANS
    John Collins; Fiscal Year: 1992
    ..These represent potential sites of action for the transposases. Finally, nucleotide sequences of sites of insertion and excision of Tc3, Tc4, and Tc5 will be determined...
  16. Creation of hyperactive transposons for mutagenesis in rodents
    Eric Ostertag; Fiscal Year: 2009
    ..The Aims will focus on synthesizing hyperactive transposases from three different families of transposons, each with unique insertion characteristics...
  17. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    ADRIENNE COTTRELL; Fiscal Year: 2007
    ..To ascertain if the transposases of the IS110/IS492 Family and Piv, the site-specific DNA recombinase, define a new family of DNA recombinases ..
  18. Engineering Transposon Mobility in Mosquitoes
    DAVID PLEDGER; Fiscal Year: 2009
    ..We further propose the evaluation of these mutant transposons using previously produced mutant Mos1 transposases in order to identify combinations of mutant transposons and transposases that display hyperactive rates of ..
  19. MUTATOR TRANSPORTABLE ELEMENTS OF MAIZE
    Virginia Walbot; Fiscal Year: 2005
    ..regulated timing of events and switch in transpositional outcome will be elucidated by analyzing proteins (MURA transposases and MURB helper proteins) encoded by the regulatory transposon MuDR...
  20. TRANSPOSABLE ELEMENTS IN CAENORHABDITIS ELEGANS
    Scott Emmons; Fiscal Year: 1991
    ..The purpose is to identify the protein factors, transposases, that catalyze the movement of these elements, and to study their regulation...
  21. Secretion systems for anti-malarial bacterial strains
    David Lampe; Fiscal Year: 2006
    ..Both Type I and Type II secretion systems will be developed and tested. [unreadable] [unreadable] [unreadable]..
  22. Characterization of V(D)J cleavage and repair complexes
    PATRICK SWANSON; Fiscal Year: 2007
    ..abstract_text> ..
  23. Genetic methods using the Tol2 transposable element
    Koichi Kawakami; Fiscal Year: 2006
    ..abstract_text> ..
  24. Transgenic analysis of Smad tumor suppressor genes
    Stuart Newfeld; Fiscal Year: 2006
    ..This knowledge can be used to determine if Smad proteins should be exploited as anti-cancer drug discovery targets. ..
  25. Gustatory and olfactory receptors of Anopheles gambiae
    Hugh Robertson; Fiscal Year: 2006
    ..This work will also contribute to our overall understanding of the molecular basis of chemoreception in insects and other animals. [unreadable] [unreadable]..
  26. MARINER TRANSPOSONS OF MAMMALS
    Hugh Robertson; Fiscal Year: 2001
    ..It will also reveal how occasionally particular copies of transposons can become integral parts of their host genetic complements. ..
  27. CHARACTERIZATION & ORGANIZATION OF TRANSPOSABLE ELEMENTS
    Zhijian Tu; Fiscal Year: 2006
    ..Finally, this work will enhance our understanding of the basic genetics of An. gambiae by systematically investigating TEs, which are substantial and potentially potent components of the genome...
  28. MATERNAL CONTROL OF ORGANIZER FORMATION IN THE ZEBRAFISH
    Eric Weinberg; Fiscal Year: 2005
    ..These studies should increase our understanding of the control of nuclear localization of beta-catenin and also allow an analysis of the initial patterning defects that result from failure to form the early dorsal signaling center. ..
  29. EVOLUTION OF IMMUNE RECOGNITION AND EVASION
    Austin L Hughes; Fiscal Year: 2010
    ..In addition, by increasing our understanding of the population diversity and evolutionary history of human immune system genes, this research will enhance our knowledge of human immune function in health and disease. ..
  30. CONJUGATION AND RECOMBINATION IN MYCOBACTERIA
    KEITH DERBYSHIRE; Fiscal Year: 2009
    ..2. To identify and characterize trans-acting transfer functions in both donor and recipient cells. 3. To examine transfer of chromosomal and plasmid DNA among fast- and slow-growing mycobacteria. ..
  31. Molecular Basis of Francisella Virulence and Immunity
    THOMAS KAWULA; Fiscal Year: 2005
    ..These aims are directed towards examining the molecular basis for F. tularensis pathogenesis, combined with an approach to elucidate potential immune response targets that could lead to improved prophylaxis against tularemia. ..
  32. Gene drive potential of transposable elements in Anopheles gambiae.
    DAVID A O apos BROCHTA; Fiscal Year: 2010
    ..gambiae will be known. This project involves the use of Animals but does not involve Human Subjects. ..
  33. Gene Duplication and Genome Evolution
    Austin Hughes; Fiscal Year: 2004
    ..5) Examining the role of transposable elements in duplication of genomic segments by testing for nonrandom association between these elements and putatively duplicated blocks in the yeast genome. ..
  34. RNAi as a modulator of arbovirus vector competence in transgenic Aedes aegypti
    ALEXANDER WILLIAM EDUARD FRANZ; Fiscal Year: 2010
    ..aegypti when they are not controlled by the mosquito's RNAi response. The proposed research will help to reveal the impact of RNAi as an antiviral defense mechanism in the midgut of Ae. aegypti. ..
  35. Molecular Evolution of Enterotoxigenic B fragilis
    AUGUSTO FRANCO MORA; Fiscal Year: 2004
    ..Our results will advance our understanding of the molecular pathogenesis of ETBF infections and will serve to delineate how ETBF originated during evolution. ..
  36. Novel genetic markers for the yellow fever mosquito
    Zhijian Tu; Fiscal Year: 2003
    ..aegypti in natural populations. ..
  37. GENETIC CONTROL OF THE YEAST SPORULATION PROGRAM
    Thomas Kornberg; Fiscal Year: 2003
    ..Learning about Ndt80 protein is likely to yield enormous insight on the decision to exit meiotic prophase and on the overall program of sporulation. ..
  38. GENETICS OF A NEWLY EVOLVED TESTES SPECIFIC GENE SDIC
    Daniel Hartl; Fiscal Year: 2003
    ..Aim 5 recognizes Sdic as a candidate gene with a possible role in postmating reproductive isolation between D. melanogaster and D. simulans, which will be tested by interspecific germline transformation. ..
  39. MOLLUSCUM CONTAGIOSUM VIRUS TOPOISOMERASE
    Frederic Bushman; Fiscal Year: 2002
    ..In addition, poxviruses have been implicated as possible biological warfare agents; thus poxvirus topoisomerase inhibitors might be use for treating such infections. ..
  40. TRANSPORT OF S-ADENOSYLMETHIONINE IN RICKETTSIA
    David Wood; Fiscal Year: 2002
    ..Completion of these studies will contribute to our understanding of how this pathogen exploits the intracellular niche as well as provide insight into rickettsial evolution. ..
  41. SCREEN FOR TUMOR SUPPRESSORS ON MOUSE CHROMOSOME 4
    Allan Bradley; Fiscal Year: 2001
    ..The pro-viral tag will facilitate the molecular identification of insertionally inactivated tumor suppressor genes in these cell lines. ..
  42. Characterization of a large protein interaction network
    Andreas Wagner; Fiscal Year: 2005
    ..The proposed work will put this speculation to an end for a key genetic network, some of whose conserved components are also involved in the etiology of human disease. ..
  43. Identification of Tetrahymena DNA Rearrangement Genes
    DOUGLAS LEE CHALKER; Fiscal Year: 2010
    ..abstract_text> ..
  44. Control of Inner Ear Development in the Zebrafish
    Eric Weinberg; Fiscal Year: 2006
    ..The fourth aim of the proposal is a continuation of our mutant screen for defects in otic vesicle formation and patterning. ..
  45. Comparative Genomics of Anopheline Mosquitoes
    Zhijian Tu; Fiscal Year: 2006
    ....
  46. Plant Transposases and their MITEs
    Susan Wessler; Fiscal Year: 2007
    ..As such, knowledge of TE-mediated mechanisms of diversification are intimately involved in improving human health and understanding how plant and animal genomes evolve. ..
  47. IMPROVED ADENOVIRAL VECTORS FOR HEPATIC GENE THERAPY
    Mark Kay; Fiscal Year: 2006
    ..Taken together, these studies will advance our basic understanding of vector-host interactions related to persistence of vector in rive, as well as advancing therapeutic applications in preclinical development. ..
  48. MOLECULAR ANALYSIS OF IS50/TN5 TRANSPOSITION
    William Reznikoff; Fiscal Year: 2006
    ..During the next grant period we will extend our genetic, biochemical and structural studies of Tn5 transposition. ..
  49. Global Analysis of the Rickettsia prowazekii Proteome
    David Wood; Fiscal Year: 2007
    ..Identification of differences in rickettsial protein expression will increase our understanding of this dynamic process as well as identify potentially new targets for vaccines and diagnostic reagents. ..
  50. MOLECULAR ANALYSIS OF THE INSERTION ELEMENT IS903
    KEITH DERBYSHIRE; Fiscal Year: 2004
    ..In particular, mutant transposases will be sought that have altered binding specificity, increased transposition activity, and the ability to ..
  51. Evolutionary Genomics of Drosophila
    Daniel Hartl; Fiscal Year: 2007
    ..abstract_text> ..
  52. Tangle Analysis of DNA Recombinases & Related Proteins
    ISABEL DARCY; Fiscal Year: 2005
    ..Apart from the recombination systems investigated in this proposal, the tangle-analysis tools that we develop will be extended to other recombinases and used to investigate related systems such as topoisomerases. ..
  53. Structure and Biochemistry of Recombinase-DNA Complexes
    PHOEBE RICE; Fiscal Year: 2008
    ..These studies will also help improve the usefulness of these recombinases as genetic tools. ..
  54. Characterization and Expression of microRNA Genes in Anopheles gambiae
    Zhijian Tu; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  55. Chemistry of HIV-1 Integrase Function
    Frederic Bushman; Fiscal Year: 2007
    ..The Specific Aims are: Aim 1. Functional studies of IN-DNA complexes Aim 2. Structural analysis of IN-DNA complexes. ..
  56. Purchase of the Roche GS SLX Sequencing System
    Frederic Bushman; Fiscal Year: 2008
    ..The instrument will be housed in the University of Pennsylvania School of Medicine DNA Sequencing Core Facility, and be operated by their staff. [unreadable] [unreadable] [unreadable]..
  57. Structural Biology of a Serine DNA Recombinase
    PHOEBE RICE; Fiscal Year: 2007
    ..It will also help in the development of more versatile genetic tools. ..
  58. THE MOLECULAR BASIS OF HAEMOPHILUS DUCREYI PATHOGENESIS
    THOMAS KAWULA; Fiscal Year: 2001
    ..The role of the exported SOS in H. ducreyi survival and lesion development will be evaluated in the swine model of chancroid using both normal and neutropenic pigs. ..
  59. Molecular recognition studies of human PB1 bromodomains
    Martin Thompson; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  60. Understanding DNA specificity in the IHF/HU family
    PHOEBE RICE; Fiscal Year: 2005
    ..Hypotheses based on comparing and contrasting such data for the 3 different proteins to be studied will be tested by site-directed mutagenesis and domain-swap experiments. ..
  61. Mechanisms of Immune Avoidance by Haemophilus ducreyi
    THOMAS KAWULA; Fiscal Year: 2007
    ..ducreyi re infection. An H. ducreyi outer membrane protein that is a target for bactericidal antibody has been identified. This protein will be tested for the ability to elicit a protective immune response to H. ducreyi. ..