Genomes and Genes
Summary: Enzymes that recombine DNA segments by a process which involves the formation of a synapse between two DNA helices, the cleavage of single strands from each DNA helix and the ligation of a DNA strand from one DNA helix to the other. The resulting DNA structure is called a Holliday junction which can be resolved by DNA REPLICATION or by HOLLIDAY JUNCTION RESOLVASES.
Publications193 found, 100 shown here
- piggyBac can bypass DNA synthesis during cut and paste transpositionRupak Mitra
Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
EMBO J 27:1097-109. 2008..This is the same strategy for target joining used by the members of DDE superfamily of transposases and retroviral integrases...
- Transposases are the most abundant, most ubiquitous genes in natureRamy K Aziz
Computation Institute, University of Chicago, Chicago, IL 60637, USA
Nucleic Acids Res 38:4207-17. 2010..archaeal, eukaryotic and viral genomes and metagenomes, and our analysis demonstrates that genes encoding transposases are the most prevalent genes in nature...
- Chromosomal transposition of PiggyBac in mouse embryonic stem cellsWei Wang
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
Proc Natl Acad Sci U S A 105:9290-5. 2008..The comprehensive information from this study should facilitate further exploration of the potential of PB and SB DNA transposons in mammalian genetics...
- Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebratesLajos Mátés
Max Delbruck Center for Molecular Medicine, Berlin, Germany
Nat Genet 41:753-61. 2009....
- Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transpositionAndrew Adey
Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
Genome Biol 11:R119. 2010..We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing...
- Messenger RNA as a source of transposase for sleeping beauty transposon-mediated correction of hereditary tyrosinemia type IAndrew Wilber
The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota, USA
Mol Ther 15:1280-7. 2007....
- Stable integration and conditional expression of electroporated transgenes in chicken embryosYuki Sato
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara, 630 0192, Japan
Dev Biol 305:616-24. 2007..Thus, the techniques proposed in this study provide a novel approach to study the mechanisms of late organogenesis, for which chickens are most suitable model animals...
- Pig transgenesis by Sleeping Beauty DNA transpositionJannik E Jakobsen
Department of Human Genetics, University of Aarhus, Wilh Meyers Allé 1240, 8000, Aarhus C, Denmark
Transgenic Res 20:533-45. 2011....
- A transposon and transposase system for human applicationPerry B Hackett
Department of Genetics, Cell Biology, and Development, Center for Genome Engineering, Institute of Human Genetics, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA
Mol Ther 18:674-83. 2010..This review examines the rationale and safety implications of application of the SB system to genetically modify T cells to be manufactured in compliance with current good manufacturing practice (cGMP) for phase I/II trials...
- Unexpectedly high copy number of random integration but low frequency of persistent expression of the Sleeping Beauty transposase after trans delivery in primary human T cellsXin Huang
Pediatric Blood and Marrow Transplantation, University of Minnesota Medical School, Minneapolis, MN 55455, USA
Hum Gene Ther 21:1577-90. 2010..Our data suggest that SB delivery via plasmid in T cells should be carried out with caution because of unexpectedly high copy numbers of randomly integrated SB transposase...
- Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transferAndrew Wilber
The Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA
Stem Cells 25:2919-27. 2007..We conclude that the SB transposon system provides an effective approach with several advantages for genetic manipulation and durable gene expression in human ES cells...
- A transposon toolkit for gene transfer and mutagenesis in protozoan parasitesJeziel D Damasceno
Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil
Genetica 138:301-11. 2010..The piggyBac and Tn5 transposons have also been shown to mobilize across Plasmodium spp. genomes circumventing the current limitations in the genetic manipulation of these organisms...
- Delivering the goods: viral and non-viral gene therapy systems and the inherent limits on cargo DNA and internal sequencesHelen Atkinson
School of Biomedical Sciences, University of Nottingham, Queen s Medical Center, Nottingham NG7 2UH, UK
Genetica 138:485-98. 2010..Length-dependence is a complex phenomenon driven not simply by the distance between the transposon ends, but by host proteins, the transposase and the properties of the DNA sequences encoded within the transposon...
- Identification and characterization of repetitive extragenic palindromes (REP)-associated tyrosine transposases: implications for REP evolution and dynamics in bacterial genomesJaroslav Nunvar
Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic
BMC Genomics 11:44. 2010..BIMEs and REPs have been shown to serve as binding sites for several proteins and suggested to play role in chromosome organization and transcription termination. Their origins are, at present, unknown...
- Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP/Cre recombinase system to distinguish transposition-specific expressionPaul R Score
Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
Mol Ther 13:617-24. 2006..The results also provide additional evidence for the effectiveness of the Sleeping Beauty transposon system as an in vivo DNA-mediated gene transfer strategy for achieving long-term expression...
- Resetting the site: redirecting integration of an insertion sequence in a predictable wayCatherine Guynet
Laboratoire de Microbiologie et Genetique Moleculaires, CNRS UMR5100, 118 Rte de Narbonne, F31062 Toulouse Cedex, France
Mol Cell 34:612-9. 2009..This suggested a way to redirect IS608 transposition to novel target sites. As we demonstrate here, we can now direct insertions in a predictable way into a variety of different chosen target sequences, both in vitro and in vivo...
- Transposon-free insertions for insect genetic engineeringGeorge C Condon
Oxitec Limited, 71 Milton Park, Oxford OX14 4RX, UK
Nat Biotechnol 24:820-1. 2006..We describe a method for post-integration elimination of all transposon sequences in the pest insect Medfly, Ceratitis capitata. The resulting insertions lack transposon sequences and are therefore impervious to transposase activity...
- Efficient mammalian germline transgenesis by cis-enhanced Sleeping Beauty transpositionDaniel F Carlson
The Center for Genome Engineering, Minneapolis, MN, USA
Transgenic Res 20:29-45. 2011..Given its simplicity, versatility and high efficiency, TnT with enhanced Sleeping Beauty components represents a compelling non-viral approach to modifying the mammalian germline...
- Hermes transposon distribution and structure in Musca domesticaRamanand A Subramanian
Center for Biosystems Research, University of Maryland Biotechnology Institute, Rockville, MD 20850, USA
J Hered 100:473-80. 2009..The simple sequence repeat found in hobo elements is a feature of this transposon that evolved since the divergence of hobo and Hermes...
- Transposition of the human Hsmar1 transposon: rate-limiting steps and the importance of the flanking TA dinucleotide in second strand cleavageCorentin Claeys Bouuaert
School of Biomedical Sciences, University of Nottingham, Queen s Medical Centre, Nottingham, UK
Nucleic Acids Res 38:190-202. 2010..Furthermore, this conformational change requires specific contacts between transposase and the flanking TA dinucleotide...
- A functional role for transposases in a large eukaryotic genomeMariusz Nowacki
Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA
Science 324:935-8. 2009..transposase genes play key roles in this process of genome-wide DNA excision, which suggests that transposases function in large eukaryotic genomes containing thousands of active transposons...
- Chimeric recombinases with designed DNA sequence recognitionAram Akopian
Institute of Biomedical and Life Sciences, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, Scotland
Proc Natl Acad Sci U S A 100:8688-91. 2003..Our results demonstrate the functional autonomy of the resolvase catalytic domain and open the way to creating "custom-built" recombinases that act at chosen natural target sequences...
- Post-integration behavior of a Mos1 mariner gene vector in Aedes aegyptiRaymond Wilson
Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742-4450, USA
Insect Biochem Mol Biol 33:853-63. 2003..aegypti for applications requiring a very high degree of vector stability but will have limited use in the construction of genetic drive, enhancer trap, or transposon tagging systems in this species...
- Active site sharing and subterminal hairpin recognition in a new class of DNA transposasesDonald R Ronning
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Mol Cell 20:143-54. 2005..Protein sequences of IS605 transposases do not bear the hallmarks of other well-characterized transposases...
- Transposons for cancer gene discovery: Sleeping Beauty and beyondLara S Collier
The Department of Genetics, Cell Biology and Development, The Cancer Center, The University of Minnesota Twin Cities, Church St SE, Minneapolis, Minnesota 55455, USA
Genome Biol 8:S15. 2007..Here, we discuss the future of Sleeping Beauty for cancer genetic studies and the potential use of additional transposable elements for somatic mutagenesis...
- Sleeping beauty transposition from nonintegrating lentivirusConrad A Vink
Institute of Child Health, University College London, UK
Mol Ther 17:1197-204. 2009..Importantly, integration site analysis revealed redirection toward a profile mimicking SB-plasmid integration and away from integration within transcriptionally active genes favored by integrase-proficient lentiviral vectors (ILVs)...
- Multiple oligomerisation domains in the IS911 transposase: a leucine zipper motif is essential for activityL Haren
Laboratoire de Microbiologie et Genetique Moleculaire du CNRS, Universite Paul Sabatier, 118 route de Narbonne, Toulouse, 31062, France
J Mol Biol 283:29-41. 1998..The two other regions are located further towards the C-terminal end of the protein, adjacent to the leucine zipper and in the region that carries the conserved catalytic DD(35)E motif...
- Hybrid lentivirus-transposon vectors with a random integration profile in human cellsNicklas H Staunstrup
Department of Human Genetics, University of Aarhus, Denmark
Mol Ther 17:1205-14. 2009..The novel hybrid vector system combines the attractive features of efficient gene delivery by viral transduction and a safer genomic integration profile by DNA transposition...
- High-level genomic integration, epigenetic changes, and expression of sleeping beauty transgeneJianhui Zhu
Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
Biochemistry 49:1507-21. 2010..In addition, long-range activation of host genes must now be recognized as a potential consequence of an inserted transgene cassette containing enhancer elements...
- Translating Sleeping Beauty transposition into cellular therapies: victories and challengesZsuzsanna Izsvák
Max Delbrück Center for Molecular Medicine MDC, Berlin, Germany
Bioessays 32:756-67. 2010..The first clinical application of the SB system will help to validate the safety of this approach...
- Shielding of sleeping beauty DNA transposon-delivered transgene cassettes by heterologous insulators in early embryonal cellsTrine Dalsgaard
Department of Human Genetics, University of Aarhus, Aarhus, Denmark
Mol Ther 17:121-30. 2009..We believe that insulated SB-derived vectors will become useful tools in transposon-based transgenesis and therapeutic gene transfer...
- DNA recognition sites activate MuA transposase to perform transposition of non-Mu DNAIlana Goldhaber-Gordon
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
J Biol Chem 277:7694-702. 2002..Multiple recognition sites help physically position MuA subunits in the transpososome plus each individual site allosterically activates transposase...
- Sequence and positional requirements for DNA sites in a mu transpososomeIlana Goldhaber-Gordon
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
J Biol Chem 277:7703-12. 2002..This variability causes only local perturbations in the protein-DNA complex, as indicated by experiments in which altered and unaltered DNA substrates are paired...
- The N-terminus of Himar1 mariner transposase mediates multiple activities during transpositionMatthew G Butler
Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA
Genetica 127:351-66. 2006..we are beginning to understand the precise mechanism of transposition of these elements, the structure of their transposases are still poorly understood...
- The mariner transposons belonging to the irritans subfamily were maintained in chordate genomes by vertical transmissionLudivine Sinzelle
, CNRS UMR 8080, IBAIC, , , Orsay Cedex, F-91405, France
J Mol Evol 62:53-65. 2006..The phylogenetic analysis of the relationships between MLE transposases reveals that Xtmar1 is closely related to Hsmar2 and Bytmar1 and that together they form a second distinct ..
- Transposon-based interferon gamma gene transfer overcomes limitations of episomal plasmid for immunogene therapy of glioblastomaA Wu
Department of Neurosurgery, University of Minnesota Medical School, Minneapolis, MN 55455, USA
Cancer Gene Ther 14:550-60. 2007..SB appears to improve the efficacy of cytokine gene therapy using nonviral vectors by enhancing the duration of transgene expression...
- Generation of an inducible and optimized piggyBac transposon systemJuan Cadinanos
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
Nucleic Acids Res 35:e87. 2007..As the activity of PB is higher than that of SB11 and SB12, two hyperactive SB transposases, we have characterized and improved the PB system in mouse ES cells...
- Identification and analysis of a hyperactive mutant form of Drosophila P-element transposaseEileen L Beall
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA
Genetics 162:217-27. 2002..These data suggest that the activity of P-element transposase may be regulated by phosphorylation and demonstrate that one mutation, S129A, results in hyperactive transposition...
- Liver-directed gene therapy using the sleeping beauty transposon systemLalitha R Belur
Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, and Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA
Methods Mol Biol 434:267-76. 2008....
- Analysis of the piggyBac transposase reveals a functional nuclear targeting signal in the 94 c-terminal residuesJames H Keith
University of Notre Dame, Notre Dame, Indiana, USA
BMC Mol Biol 9:72. 2008..A PSORTII analysis of the TPase amino acid sequence predicts a bipartite nuclear localization signal (NLS) near the c-terminus, just upstream of a putative ZnF (ZnF)...
- Abundant transposases encoded by the metagenome of a hydrothermal chimney biofilmWilliam J Brazelton
School of Oceanography and Center for Astrobiology and Early Evolution, University of Washington, Seattle, WA 98195, USA
ISME J 3:1420-4. 2009..More than 8% of all metagenomic reads showed significant sequence similarity to transposases; all available metagenomic data sets from other environments contained at least an order of magnitude fewer ..
- Mariner Mos1 transposase optimization by rational mutagenesisStephanie Germon
GICC, Universite Francois Rabelais de Tours, 37200, Tours, France
Genetica 137:265-76. 2009..Even if host factors are not essential for transposition, our data demonstrate that the host machinery is essential in regulating MOS1 activity...
- DNA recognition and the precleavage state during single-stranded DNA transposition in D. radioduransAlison Burgess Hickman
Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
EMBO J 29:3840-52. 2010Bacterial insertion sequences (ISs) from the IS200/IS605 family encode the smallest known DNA transposases and mobilize through single-stranded DNA transposition...
- A simple topological filter in a eukaryotic transposon as a mechanism to suppress genome instabilityCorentin Claeys Bouuaert
School of Biomedical Sciences, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2UH, United Kingdom
Mol Cell Biol 31:317-27. 2011..This provides constraints on the enzymatic mechanism of the double-strand breaks in mariner transposition, excluding the most prevalent of the current models...
- The mariner Mos1 transposase produced in tobacco is active in vitroXavier Thomas
Universite Francois Rabelais de Tours, GICC, Parc de Grandmont, 37200 Tours, France
Genetica 138:519-30. 2010..Several hypotheses are proposed that could explain the inhibition of HMNtMOS1 activity in planta...
- A novel DNA binding and nuclease activity in domain III of Mu transposase: evidence for a catalytic region involved in donor cleavageZ Wu
Department of Biochemistry, University of Western Ontario, London, Canada
EMBO J 14:3835-43. 1995..This proposal for active site assembly is in agreement with the recently proposed domain sharing model by Yang et al. (Yang, J.Y., Kim, K., Jayaram, M. and Harshey, R.M.  EMBO J., 14, 2374-2384)...
- Transposase-transposase interactions in MOS1 complexes: a biochemical approachGuillaume Carpentier
Universite Francois Rabelais de Tours, GICC, CNRS, UMR 6239, UFR Sciences and Techniques, Parc Grandmont, 37200 Tours, France
J Mol Biol 405:892-908. 2011b>Transposases are proteins that have assumed the mobility of class II transposable elements...
- The catalytic domain of all eukaryotic cut-and-paste transposase superfamiliesYao Wu Yuan
Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
Proc Natl Acad Sci U S A 108:7884-9. 2011..g., hAT, P) based on sequence similarity of the element-encoded transposase. The transposases from several superfamilies possess a protein domain containing an acidic amino acid triad (DDE or DDD) that ..
- In vitro recombination and inverted terminal repeat binding activities of the Mcmar1 transposaseSylvaine Renault
Universite Francois Rabelais de Tours, GICC, CNRS, UMR 6239, UFR des Sciences and Techniques, Parc de Grandmont, 37200 Tours, France
Biochemistry 49:3534-44. 2010..This suggests that the assembly of the transposition complex is more complex than that of those involved in the mobility of the Mos1 and Himar1 mariner elements...
- Duration of expression and activity of Sleeping Beauty transposase in mouse liver following hydrodynamic DNA deliveryJason B Bell
Department of Genetics, University of Minnesota, Minneapolis, Minnesota, USA
Mol Ther 18:1796-802. 2010..Thus, within the limits of current technology, we show that SB transposons appear to be as stably integrated as their viral counterparts...
- Transposition of ISHp608, member of an unusual family of bacterial insertion sequencesBao Ton-Hoang
, CNRS UMR5100, Toulouse Cedex, France
EMBO J 24:3325-38. 2005..We also demonstrate that TnpA, which is less than half the length of other transposases, is responsible for this and for ISHp608 transposition...
- An in vivo transposase-catalyzed single-stranded DNA circularization reactionP Polard
Laboratoire de Microbiologie et Genetique Moleculaires, UPR9007 du Centre National de la Resherche Scientifique CNRS, Toulouse, France
Genes Dev 9:2846-58. 1995..Therefore, IS911 excision as a circle may not occur by double-strand cleavage leading to its prior separation from the vector backbone in a linear form but could proceed by consecutive circularization of each strand...
- In vitro reconstitution of a single-stranded transposition mechanism of IS608Catherine Guynet
Laboratoire de Microbiologie et Genetique Moleculaires, CNRS UMR5100, 118 Rte de Narbonne, F31062 Toulouse Cedex, France
Mol Cell 29:302-12. 2008..This single-strand transposition mode has important implications not only for dispersion of IS608 but also for the other members of this very large IS family...
- Conservation of Palindromic and Mirror Motifs within Inverted Terminal Repeats of mariner-like ElementsYves Bigot
Laboratoire d Etude des Parasites Génétiques, Universite Francois Rabelais, E A 3868, UFR des Sciences et Techniques, Parc de Grandmont, Avenue Monge, 37200 Tours, France
J Mol Biol 351:108-16. 2005..ITR) are therefore recognized, as previously indicated, by biochemical data obtained with the Mos1 and Himar1 transposases. Here, we define the motifs that are involved in the binding of a MLE transposase to its ITR by analyzing the ..
- Microbial co-habitation and lateral gene transfer: what transposases can tell usSean D Hooper
Department of Energy Joint Genome Institute, Genome Biology Program, Mitchell Drive, Walnut Creek, CA 94598, USA
Genome Biol 10:R45. 2009..In this study, we explore an approach using the history of lateral gene transfer recorded in microbial genomes to begin to answer two key questions: where have you been and who have you been with?..
- Assembly of the Tc1 and mariner transposition initiation complexes depends on the origins of their transposase DNA binding domainsBenjamin Brillet
Laboratoire d Etudes des Parasites Génétiques, Universite Francois Rabelais, FRE CNRS 2969, UFR Sciences and Techniques, Parc Grandmont, 37200, Tours, France
Genetica 130:105-20. 2007..Elements belonging to this super-family encode transposases with DNA binding domains of different origins, and recent data indicate that the chimerization of functional ..
- Sleeping Beauty transposase modulates cell-cycle progression through interaction with Miz-1Oliver Walisko
Max Delbruck Center for Molecular Medicine, D 13092 Berlin, Germany
Proc Natl Acad Sci U S A 103:4062-7. 2006..Because nonhomologous end-joining is required for efficient SB transposition, the transposase-induced G(1) slowdown is probably a selfish act on the transposon's part to maximize the chance for a successful transposition event...
- Dissecting Tn5 transposition using HIV-1 integrase diketoacid inhibitorsAgata Czyz
Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706, USA
Biochemistry 46:10776-89. 2007..Thus, DKA inhibitors will provide an important set of tools to investigate the mechanism of action of transposases and integrases.
- DNA transposons in vertebrate functional genomicsC Miskey
, , 13092, Berlin, Germany
Cell Mol Life Sci 62:629-41. 2005..We put emphasis on the evolution and regulation of members of the best-characterized and most widely used Tc1/mariner family...
- Characterization of multiple lineages of Tc1-like elements within the genome of the amphibian Xenopus tropicalisLudivine Sinzelle
, CNRS UMR 8080, IBAIC, , , F-91405 Orsay Cedex, France
Gene 349:187-96. 2005..Xeminos is the first vertebrate TLE strongly related to an invertebrate lineage. Finally, we have identified for most of these TLEs, copies containing an intact transposase ORF suggesting that these elements may still be active...
- Transposons reanimated in miceTimothy H Bestor
Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA
Cell 122:322-5. 2005..In either case, the future of transposon-driven genetic analyses of mice and other mammals looks promising...
- Family of Tc1-like elements from fish genomes and horizontal transferAnita Pocwierz Kotus
Department of Genetics and Marine Biotechnology, Institute of Oceanology, Polish Academy of Sciences, Sw Wojciecha 5, 81 347 Gdynia, Poland
Gene 390:243-51. 2007..Further similar studies would help to explain the evolution of the multiple Tc1 lineages including species for which full genome sequences will not be available soon...
- Gene transfer into genomes of human cells by the sleeping beauty transposon systemAron M Geurts
Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota 55455, USA
Mol Ther 8:108-17. 2003..With the recently described improvements to the transposon itself, the SB system appears to be a potential gene-transfer tool for human gene therapy...
- Subtraction by addition: domesticated transposases in programmed DNA eliminationJason A Motl
Biology Department, Washington University in St Louis, St Louis, Missouri 63130, USA
Genes Dev 23:2455-60. 2009..This new study reveals how addition of a transposase to small RNA-directed silencing machinery can guide major genome reorganization...
- Transposition and target specificity of the typical IS30 family element IS1655 from Neisseria meningitidisJanos Kiss
Agricultural Biotechnology Center, Szent Gyorgyi Albert u 4, H 2100, Godollo, Hungary
Mol Microbiol 63:1731-47. 2007..Moreover, it shows striking similarity to IS30 in transposition strategy even though their transposases differ in their N-terminal regions, which, for IS30, appears to determine target specificity...
- Two-metal active site binding of a Tn5 transposase synaptic complexScott Lovell
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
Nat Struct Biol 9:278-81. 2002..The position of the metal ions and the conformational changes of the DNA provide insight into the mechanism of hairpin formation and cleavage, and is consistent with a two-metal model for catalysis...
- Unexpected structural diversity in DNA recombination: the restriction endonuclease connectionA B Hickman
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell 5:1025-34. 2000..This result indicates that the range of biological processes that utilize restriction enzyme-like folds also includes DNA transposition...
- When phage, plasmids, and transposons collide: genomic islands, and conjugative- and mobilizable-transposons as a mosaic continuumA Mark Osborn
Department of Biological Sciences, University of Essex, Colchester, CO4 3SQ, UK
Plasmid 48:202-12. 2002..This review highlights both the similarities and distinctions between these integrating transferable elements resulting from combination of the MGE toolbox...
- A Coxiella burnetti repeated DNA element resembling a bacterial insertion sequenceT A Hoover
Bacteriology Division, U S Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702 5011
J Bacteriol 174:5540-8. 1992..Despite the sequence changes, all three copies of the element have retained significant dyad symmetry in this region...
- Target DNA structure plays a critical role in RAG transpositionJennifer E Posey
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America
PLoS Biol 4:e350. 2006..These findings suggest novel regulatory possibilities and may provide insight into the activities of other transposases.
- Sub-terminal sequences modulating IS30 transposition in vivo and in vitroMonika Szabo
Agricultural Biotechnology Center, 4 Szent Györgyi Albert Str, H 2100, Godollo, Hungary
J Mol Biol 375:337-52. 2008..In vitro kinetic experiments suggest that the sub-terminal enhancers may, at least partly, be transposase-dependent. Such enhancers may reflect a subtle regulatory mechanism for IS30 transposition...
- Complete sequence determination combined with analysis of transposition/site-specific recombination events to explain genetic organization of IncP-7 TOL plasmid pWW53 and related mobile genetic elementsHirokazu Yano
Graduate School of Life Sciences, Tohoku University, Katahira, Sendai 980 8577, Japan
J Mol Biol 369:11-26. 2007....
- Diverse Mariner-like elements in fig waspsE R Haine
Division of Biology, Imperial College London, Silwood Park, Ascot, Berkshire, UK
Insect Mol Biol 16:743-52. 2007..The association between mariner elements and fig wasps is old and dominated by vertical transmission, suggesting that these 'selfish genetic elements' have evolved to impart only very low costs to their hosts...
- ISWpi1 from Wolbachia pipientis defines a novel group of insertion sequences within the IS5 familyRichard Cordaux
Laboratoire de Génétique et Biologie des Populations de Crustacés, CNRS UMR 6556, Universite de Poitiers, 40 Avenue du Recteur Pineau, 86022 Poitiers, France
Gene 409:20-7. 2008..pipientis bacteria, especially in light of the increasing interest in developing these bacteria as tools for controlling insect disease vectors and agricultural pests...
- Mechanism of IS200/IS605 family DNA transposases: activation and transposon-directed target site selectionOrsolya Barabas
Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Cell 132:208-20. 2008The smallest known DNA transposases are those from the IS200/IS605 family. Here we show how the interplay of protein and DNA activates TnpA, the Helicobacter pylori IS608 transposase, for catalysis...
- Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genesLudivine Sinzelle
Max Delbruck Center for Molecular Medicine, 13092 Berlin, Germany
Proc Natl Acad Sci U S A 105:4715-20. 2008..The Harbinger transposon will serve as a useful experimental system for transposon biology and for investigating the enzymatic functions of domesticated, transposon-derived cellular genes...
- Transcribed Tc1-like transposons in salmonid fishAleksei Krasnov
Institute of Applied Biotechnology, University of Kuopio, P, O, B, 1627, FIN 70211 Kuopio, Finland
BMC Genomics 6:107. 2005..High throughput sequencing of salmonid fish cDNA libraries has revealed a large number of transposons, which remain transcribed despite inactivation of translation. This article reports on the structure and potential role of these genes...
- In vivo random mutagenesis of Bacillus subtilis by use of TnYLB-1, a mariner-based transposonYoann Le Breton
Department of Microbiology and Immunology, MSC 7758, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229 3900, USA
Appl Environ Microbiol 72:327-33. 2006..Two auxotrophic mutants were identified among the 200 transposants, one with an insertion in lysA and another in a gene (yjzB) whose function is unknown...
- Translocation of transposition-deficient (TndPKLH2-like) transposons in the natural environment: mechanistic insights from the study of adjacent DNA sequencesGennady Kholodii
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia
Microbiology 150:979-92. 2004..On the basis of DNA sequence data, possible mechanisms of translocation of defective Tn(d)PKLH2-like elements via recombination events implicating the nearby res (resolution) site and IS element are proposed...
- Congruence of in vivo and in vitro insertion patterns in hot E. coli gene targets of transposable element Mu: opposing roles of MuB in target capture and integrationJun Ge
Section of Molecular Genetics and Microbiology and Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
J Mol Biol 380:598-607. 2008..Actual integration events are then directed to sites that are in proximity to MuB filaments but are themselves free of MuB...
- Characterization of a small mobilizable transposon, MTnSag1, in Streptococcus agalactiaeAdeline Achard
CHU Cote de Nacre, Universite de Caen Basse Normandie, 14033 Caen Cedex, France
J Bacteriol 189:4328-31. 2007..MTnSag1 could be mobilized by the conjugative transposon Tn916. An intermediate circular form of MTnSag1 and a putative origin of transfer at the 3' end of the lnu(C) gene were characterized...
- Chimeric Mos1 and piggyBac transposases result in site-directed integrationK J Maragathavally
Department of Entomology, Texas A and M University, College Station, Texas, USA
FASEB J 20:1880-2. 2006..We developed a novel technology that utilizes chimeric transposases to direct integration into specific sites on a target DNA molecule...
- Mos1-mediated insertional mutagenesis in Caenorhabditis elegansThomas Boulin
ENS, 46 rue d Ulm, F 75005 Paris, France
Nat Protoc 2:1276-87. 2007..The recent development of targeted engineering of C. elegans loci carrying Mos1 insertions further increases the attractiveness of Mos1-mediated mutagenesis...
- Tol2 transposon-mediated transgenesis in Xenopus tropicalisMichelle R Johnson Hamlet
Department of Pathology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
Genesis 44:438-45. 2006..Here, we show that the Medaka fish transposon, Tol2, is able to stably integrate into the X. tropicalis genome and will serve as a powerful tool for insertional mutagenesis strategies in the frog...
- Targeted Sleeping Beauty transposition in human cellsZoltan Ivics
Max Delbruck Center for Molecular Medicine, Berlin, Germany
Mol Ther 15:1137-44. 2007..Targeted transposition can be a powerful technology for safe transgene integration in human therapeutic applications...
- Functional characterization of IS1999, an IS4 family element involved in mobilization and expression of beta-lactam resistance genesDaniel Aubert
Service de Bacteriologie Virologie, Hopital de Bicetre, 78 rue du General Leclerc, 94275 Le Kremlin Bicetre cedex, France
J Bacteriol 188:6506-14. 2006..This reduced transposition frequency of IS1999.2-based elements may result from a lower transcription of the transposase gene, as revealed by reverse transcription-PCR analyses...
- Positive selection on transposase genes of insertion sequences in the Crocosphaera watsonii genomeTed H M Mes
Netherlands Institute of Ecology NIOO KNAW, Centre for Estuarine and Marine Ecology, POB 140, 4400 AC Yerseke, The Netherlands
J Bacteriol 188:7176-85. 2006..Intragenic adaptation of IS-carried genes identified here may constitute a novel mechanism associated with bacterial diversification and adaptation...
- Synapsis of Tn3 recombination sites: unpaired sites destabilize synapses by a partner exchange mechanismJiuya He
Institute of Biomedical and Life Sciences, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, UK
J Mol Biol 319:385-93. 2002..This mechanism reconciles studies on selective synapsis in multi-res substrates, which imply rapid interchange of synaptic pairings, with studies indicating that synapses of two Tn3res sites are stable...
- DNA-binding activity and subunit interaction of the mariner transposaseL Zhang
Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK
Nucleic Acids Res 29:3566-75. 2001..This mutant enzyme allows insertion at dinucleotides other than TA, including sequences with GC base pairs. This is the first example of a mariner/Tc1 transposase with altered target specificity...
- Rapid inactivation of the maize transposable element En/Spm in Medicago truncatulaV Cosson
, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France
Mol Genet Genomics 269:732-45. 2003..thaliana and even lower in M. truncatula. The use of different sources of transposases suggested that this defect in transposition was associated with the dSpm element itself...
- Transposition and gene disruption in the male germline of the mouseA J Dupuy
Beckman Center for Transposon Research, University of Minnesota Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
Genesis 30:82-8. 2001..This technique has promise as a new strategy for forward genetic screens in the mouse or functional genomics...
- ISZm1068: an IS5-like insertion element from Zymomonas mobilisM Galeros
Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Panepistemiopolis, Kouponia, Greece
Arch Microbiol 175:323-33. 2001..both at the nucleotide and at the amino acid sequence level with the corresponding ORFs encoding the transposases of many IS5 family elements, in particular the IS1031 group. Moreover, the Z...
- Determinants for hairpin formation in Tn10 transpositionJ S Allingham
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5B7
EMBO J 20:2931-42. 2001..In contrast, a mutation at another conserved position, W265, is shown to relax the specificity of the hairpin formation reaction...
- Genetic footprinting in bacteriaR S Hare
Schering Plough Research Institute, Kenilworth, New Jersey 07033, USA
J Bacteriol 183:1694-706. 2001..In addition, the ability of recombinant proteins to complement mutagenized hosts has been evaluated by genetic footprinting using a bacteriophage lambda transposon delivery system...
- Genomic characterization of Rim2/Hipa elements reveals a CACTA-like transposon superfamily with unique features in the rice genomeG-D Wang
SHARF and National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032 Shanghai, China
Mol Genet Genomics 270:234-42. 2003..analysis indicates that the putative RIM2 proteins fall into a subgroup distinct from the TNP2-like subgroup of transposases. Southern hybridization with genomic DNA from monocotyledonous and dicotyledonous plants demonstrates that the ..
- The Mu repressor-DNA complex contains an immobilized 'wing' within the minor grooveJ M Wojciak
Department of Chemistry and Biochemistry, Molecular Biology Institute and the UCLA-DOE Laboratory of Structural Biology and Molecular Medicine, University of California, Los Angeles 90095, USA
Nat Struct Biol 8:84-90. 2001..Extensive contacts are also formed between the recognition helix and the DNA, which reads the major groove of a highly conserved region of the binding site through a single base-specific hydrogen bond and van der Waals contacts...
- Detection and analysis of transpositionally active head-to-tail dimers in three additional Escherichia coli IS elementsIldiko Szeverenyi
Environmental Biosafety Research Institute, Agricultural Biotechnology Centre, Szent Györgyi Albert str 4, H 2101 Gödöllõ, Hungary
Microbiology 149:1297-310. 2003..The results presented for IS3, IS150 and IS186 are in accordance with the IS dimer model, which is in turn consistent with models based on covalently closed minicircles...
- The DNA-bending protein HMGB1 is a cellular cofactor of Sleeping Beauty transpositionHatem Zayed
, , D-13092 Berlin, Germany
Nucleic Acids Res 31:2313-22. 2003....
- Transposase and cointegrase: specialized transposition proteins of the bacterial insertion sequence IS21 and related elementsB Berger
Laboratoire de Biologie Microbienne, , Switzerland
Cell Mol Life Sci 58:403-19. 2001..This review also includes a survey of the IS21 family and speculates on the possibility that other members present a similar transpositional specialization...
- Transposition of cyanobacterium insertion element ISY100 in Escherichia coliAkihiro Urasaki
Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan
J Bacteriol 184:5104-12. 2002..ISY100 transposes in E. coli, indicating that it transposes without any host factor other than the transposase encoded by itself. Therefore, it may be able to transpose in other biological systems...
- Sin recombinase from Staphylococcus aureus: synaptic complex architecture and transposon targetingSally J Rowland
Institute of Biomedical and Life Sciences, University of Glasgow, Anderson College, UK
Mol Microbiol 44:607-19. 2002..Our model for the recombination synapse has at its core an assembly of four Sin dimers; Hbsu plays an architectural role that is taken by two resolvase dimers in models of the Tn3/gammadelta synapse...
- BRUCELLA VACCINE FOR BIOTERRORISMGary Splitter; Fiscal Year: 2004..Included in these islands are ORFs encoding hypothetical proteins, transporters, transposases, and transcriptional regulators that most likely aid successful establishment of Brucella in human infections...
- GENE AMPLIFICATION BY A TRANSPOSITION APPARATUSHiroshi Nakai; Fiscal Year: 1999..the component that catalyzes strand transfer, performing a function similar to retroviral integrases and other transposases. However, the participation of the Mu A protein is not over after catalyzing strand transfer...
- Genetic transformation system for Chlamydia suisDANIEL ROCKEY; Fiscal Year: 2007..the island encode plasmid mobilization and replication functions, tetracycline efflux pump, and candidate transposases. Experimental data in a surrogate system suggests that one of these transposases was responsible for the ..
- Plant Transposases and their MITEsSusan Wessler; Fiscal Year: 2007..As such, knowledge of TE-mediated mechanisms of diversification are intimately involved in improving human health and understanding how plant and animal genomes evolve. ..
- A gene-trap screen for hearing and balanceDONNA FEKETE; Fiscal Year: 2007..Pseudotyped retroviral vectors or Tol2 transposases will be used to insert a gene-trap construct into the zebrafish germline...
- TRANSPOSASES IN ETOPOSIDE RESISTANCERobert A Hromas; Fiscal Year: 2010..In addition, the levels of Metnase could predict which patient will respond to etoposide. ..
- MECHANISTIC ANALYSIS OF DNA TRANSPOSITION BY PHAGE MUTania Baker; Fiscal Year: 2001..become clear that Mu transposase is a member of a structurally related family of proteins that includes many transposases and the retroviral integrases. These proteins catalyze recombination as multimeric complexes bound to DNA...
- MINORITY PREDOCTORAL FELLOWSHIP PROGRAMADRIENNE COTTRELL; Fiscal Year: 2007..To ascertain if the transposases of the IS110/IS492 Family and Piv, the site-specific DNA recombinase, define a new family of DNA recombinases ..
- MUTATOR TRANSPORTABLE ELEMENTS OF MAIZEVirginia Walbot; Fiscal Year: 2005..regulated timing of events and switch in transpositional outcome will be elucidated by analyzing proteins (MURA transposases and MURB helper proteins) encoded by the regulatory transposon MuDR...
- MOLECULAR ANALYSIS OF THE INSERTION ELEMENT IS903KEITH DERBYSHIRE; Fiscal Year: 2004..In particular, mutant transposases will be sought that have altered binding specificity, increased transposition activity, and the ability to ..
- BACTERIOPHAGE MU DNA TRANSPOSITIONRasika Harshey; Fiscal Year: 1992..We propose experiments that specifically address the post-transcriptional regulation of A- protein synthesis. We hope to uncover novel ways in which transposases limit their expression and thereby their mutagenic potential.
- Human DNA transposons: evolutionary history and genomic impactCEDRIC FESCHOTTE; Fiscal Year: 2007..The second aim of this research is to assess the enzymatic activities of two transposases, MAR and PGBD3, which are potentially expressed in several human tissues and were identified as being among the ..
- Human DNA transposons: evolutionary history and genomic impactCEDRIC FESCHOTTE; Fiscal Year: 2010..The second aim of this research is to assess the enzymatic activities of two transposases, MAR and PGBD3, which are potentially expressed in several human tissues and were identified as being among the ..
- MUTATOR TRANSPORTABLE ELEMENTS OF MAIZEVirginia Walbot; Fiscal Year: 2001..In vitro biochemical studies will examine the DNA binding properties and enzymatic activities of the transposes encoded by MuDR to determine if different transposases are required for "cut and paste" and replicative transposition.
- Secretion systems for anti-malarial bacterial strainsDavid Lampe; Fiscal Year: 2006..agglomerans that secrete antimalarial proteins can block the transmission of malaria from mosquitoes to their hosts in a rodent malaria model system. Both Type I and Type II secretion systems will be developed and tested. ..
- Genetic methods using the Tol2 transposable elementKoichi Kawakami; Fiscal Year: 2006..abstract_text> ..
- Characterization of V(D)J cleavage and repair complexesPATRICK SWANSON; Fiscal Year: 2007..abstract_text> ..
- Gustatory and olfactory receptors of Anopheles gambiaeHugh Robertson; Fiscal Year: 2006..This work will also contribute to our overall understanding of the molecular basis of chemoreception in insects and other animals. ..
- MARINER TRANSPOSONS OF MAMMALSHugh Robertson; Fiscal Year: 2001..It will also reveal how occasionally particular copies of transposons can become integral parts of their host genetic complements. ..
- THE MOLECULAR BASIS OF HAEMOPHILUS DUCREYI PATHOGENESISTHOMAS KAWULA; Fiscal Year: 2001..The role of the exported SOS in H. ducreyi survival and lesion development will be evaluated in the swine model of chancroid using both normal and neutropenic pigs. ..
- MOLECULAR ANALYSIS OF IS50/TN5 TRANSPOSITIONWilliam Reznikoff; Fiscal Year: 2003..During the next grant period we will extend our genetic, biochemical and structural studies of Tn5 transposition. ..
- Structural Biology of a Serine DNA RecombinasePHOEBE RICE; Fiscal Year: 2007..It will also help in the development of more versatile genetic tools. ..
- Molecular recognition studies of human PB1 bromodomainsMartin Thompson; Fiscal Year: 2007....
- Mechanisms of Immune Avoidance by Haemophilus ducreyiTHOMAS KAWULA; Fiscal Year: 2007..ducreyi re infection. An H. ducreyi outer membrane protein that is a target for bactericidal antibody has been identified. This protein will be tested for the ability to elicit a protective immune response to H. ducreyi. ..
- Understanding DNA specificity in the IHF/HU familyPHOEBE RICE; Fiscal Year: 2005..Hypotheses based on comparing and contrasting such data for the 3 different proteins to be studied will be tested by site-directed mutagenesis and domain-swap experiments. ..