transposases

Summary

Summary: Enzymes that recombine DNA segments by a process which involves the formation of a synapse between two DNA helices, the cleavage of single strands from each DNA helix and the ligation of a DNA strand from one DNA helix to the other. The resulting DNA structure is called a Holliday junction which can be resolved by DNA REPLICATION or by HOLLIDAY JUNCTION RESOLVASES.

Top Publications

  1. pmc piggyBac can bypass DNA synthesis during cut and paste transposition
    Rupak Mitra
    Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
    EMBO J 27:1097-109. 2008
  2. pmc Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Methods 6:363-9. 2009
  3. doi Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates
    Lajos Mátés
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Genet 41:753-61. 2009
  4. ncbi Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells
    Z Ivics
    Department of Genetics and Cell Biology, University of Minnesota, St Paul 55108 1095, USA
    Cell 91:501-10. 1997
  5. ncbi Disassembly of the Mu transposase tetramer by the ClpX chaperone
    I Levchenko
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139, USA
    Genes Dev 9:2399-408. 1995
  6. pmc Gene transfer efficiency and genome-wide integration profiling of Sleeping Beauty, Tol2, and piggyBac transposons in human primary T cells
    Xin Huang
    Division of Pediatric Blood and Marrow Transplantation, University of Minnesota Medical School, Minneapolis, Minnesota, USA
    Mol Ther 18:1803-13. 2010
  7. pmc Integrating prokaryotes and eukaryotes: DNA transposases in light of structure
    Alison Burgess Hickman
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD 20892, USA
    Crit Rev Biochem Mol Biol 45:50-69. 2010
  8. pmc Genome-wide analysis of mariner-like transposable elements in rice reveals complex relationships with stowaway miniature inverted repeat transposable elements (MITEs)
    CEDRIC FESCHOTTE
    Department of Plant Biology, The University of Georgia, Athens, Georgia 30602, USA
    Genetics 163:747-58. 2003
  9. doi Delivering the goods: viral and non-viral gene therapy systems and the inherent limits on cargo DNA and internal sequences
    Helen Atkinson
    School of Biomedical Sciences, University of Nottingham, Queen s Medical Center, Nottingham NG7 2UH, UK
    Genetica 138:485-98. 2010
  10. pmc ClpX protein of Escherichia coli activates bacteriophage Mu transposase in the strand transfer complex for initiation of Mu DNA synthesis
    R Kruklitis
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    EMBO J 15:935-44. 1996

Research Grants

  1. BRUCELLA VACCINE FOR BIOTERRORISM
    Gary Splitter; Fiscal Year: 2004
  2. GENE AMPLIFICATION BY A TRANSPOSITION APPARATUS
    Hiroshi Nakai; Fiscal Year: 1999
  3. Genetic transformation system for Chlamydia suis
    DANIEL ROCKEY; Fiscal Year: 2007
  4. Plant Transposases and their MITEs
    Susan Wessler; Fiscal Year: 2007
  5. A gene-trap screen for hearing and balance
    DONNA FEKETE; Fiscal Year: 2007
  6. TRANSPOSASES IN ETOPOSIDE RESISTANCE
    Robert A Hromas; Fiscal Year: 2010
  7. MECHANISTIC ANALYSIS OF DNA TRANSPOSITION BY PHAGE MU
    Tania Baker; Fiscal Year: 2001
  8. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    ADRIENNE COTTRELL; Fiscal Year: 2007
  9. MUTATOR TRANSPORTABLE ELEMENTS OF MAIZE
    Virginia Walbot; Fiscal Year: 2005
  10. MOLECULAR ANALYSIS OF THE INSERTION ELEMENT IS903
    KEITH DERBYSHIRE; Fiscal Year: 2004

Detail Information

Publications238 found, 100 shown here

  1. pmc piggyBac can bypass DNA synthesis during cut and paste transposition
    Rupak Mitra
    Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
    EMBO J 27:1097-109. 2008
    ..This is the same strategy for target joining used by the members of DDE superfamily of transposases and retroviral integrases...
  2. pmc Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Methods 6:363-9. 2009
    ..iPSCs fulfilled all criteria of pluripotency, such as pluripotency gene expression, teratoma formation and contribution to chimeras. piggyBac transposon-based reprogramming may be used to generate therapeutically applicable iPSCs...
  3. doi Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates
    Lajos Mátés
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Genet 41:753-61. 2009
    ....
  4. ncbi Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells
    Z Ivics
    Department of Genetics and Cell Biology, University of Minnesota, St Paul 55108 1095, USA
    Cell 91:501-10. 1997
    ..Sleeping Beauty is an active DNA-transposon system from vertebrates for genetic transformation and insertional mutagenesis...
  5. ncbi Disassembly of the Mu transposase tetramer by the ClpX chaperone
    I Levchenko
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139, USA
    Genes Dev 9:2399-408. 1995
    ..These data contribute to the emerging picture that members of the Clp family are chaperones specifically suited for disaggregating proteins and are able to function with or without a collaborating protease...
  6. pmc Gene transfer efficiency and genome-wide integration profiling of Sleeping Beauty, Tol2, and piggyBac transposons in human primary T cells
    Xin Huang
    Division of Pediatric Blood and Marrow Transplantation, University of Minnesota Medical School, Minneapolis, Minnesota, USA
    Mol Ther 18:1803-13. 2010
    ....
  7. pmc Integrating prokaryotes and eukaryotes: DNA transposases in light of structure
    Alison Burgess Hickman
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD 20892, USA
    Crit Rev Biochem Mol Biol 45:50-69. 2010
    ..DNA transposases comprise one class of such enzymes...
  8. pmc Genome-wide analysis of mariner-like transposable elements in rice reveals complex relationships with stowaway miniature inverted repeat transposable elements (MITEs)
    CEDRIC FESCHOTTE
    Department of Plant Biology, The University of Georgia, Athens, Georgia 30602, USA
    Genetics 163:747-58. 2003
    ..These data suggest a model whereby most of the Stowaway MITEs in rice were cross-mobilized by MLE transposases encoded by distantly related elements.
  9. doi Delivering the goods: viral and non-viral gene therapy systems and the inherent limits on cargo DNA and internal sequences
    Helen Atkinson
    School of Biomedical Sciences, University of Nottingham, Queen s Medical Center, Nottingham NG7 2UH, UK
    Genetica 138:485-98. 2010
    ..Length-dependence is a complex phenomenon driven not simply by the distance between the transposon ends, but by host proteins, the transposase and the properties of the DNA sequences encoded within the transposon...
  10. pmc ClpX protein of Escherichia coli activates bacteriophage Mu transposase in the strand transfer complex for initiation of Mu DNA synthesis
    R Kruklitis
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    EMBO J 15:935-44. 1996
    ..These results indicate that ClpX protein activates MuA in STC1 so that it can recruit crucial host factors needed to initiate Mu DNA synthesis by specific replication enzymes...
  11. pmc The AAA+ ClpX machine unfolds a keystone subunit to remodel the Mu transpososome
    Aliaa H Abdelhakim
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 107:2437-42. 2010
    ..The specific targeting of a stabilizing "keystone subunit" within a complex for unfolding is an attractive general mechanism for remodeling by AAA+ enzymes...
  12. pmc Manipulating piggyBac transposon chromosomal integration site selection in human cells
    Claudia Kettlun
    Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Ther 19:1636-44. 2011
    ..Our results offer insight into the requirements for using a chimeric zinc finger-piggyBac transposase to direct integration into a user-defined chromosomal location...
  13. pmc Identification and characterization of repetitive extragenic palindromes (REP)-associated tyrosine transposases: implications for REP evolution and dynamics in bacterial genomes
    Jaroslav Nunvar
    Department of Genetics and Microbiology, Faculty of Science, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic
    BMC Genomics 11:44. 2010
    ..BIMEs and REPs have been shown to serve as binding sites for several proteins and suggested to play role in chromosome organization and transcription termination. Their origins are, at present, unknown...
  14. pmc Site-directed transposon integration in human cells
    Stephen R Yant
    Department of Pediatrics and Genetics, Stanford University School of Medicine, Stanford, CA 94305 5208, USA
    Nucleic Acids Res 35:e50. 2007
    ..These findings illustrate the feasibility of directed transposon integration and highlight potential means for future development...
  15. pmc Trans-kingdom transposition of the maize dissociation element
    Alexander Emelyanov
    Temasek Life Sciences Laboratory, The National University of Singapore, 117604 Singapore, Singapore
    Genetics 174:1095-104. 2006
    ....
  16. pmc Transposases are the most abundant, most ubiquitous genes in nature
    Ramy K Aziz
    Computation Institute, University of Chicago, Chicago, IL 60637, USA
    Nucleic Acids Res 38:4207-17. 2010
    ..archaeal, eukaryotic and viral genomes and metagenomes, and our analysis demonstrates that genes encoding transposases are the most prevalent genes in nature...
  17. pmc Convergent domestication of pogo-like transposases into centromere-binding proteins in fission yeast and mammals
    Claudio Casola
    Department of Biology, University of Texas, Arlington, USA
    Mol Biol Evol 25:29-41. 2008
    ..Each of these proteins displays extensive sequence similarity with pogo-like transposases, which have been previously identified in the genomes of various insects and vertebrates, in the protozoan ..
  18. pmc Transposition of the human Hsmar1 transposon: rate-limiting steps and the importance of the flanking TA dinucleotide in second strand cleavage
    Corentin Claeys Bouuaert
    School of Biomedical Sciences, University of Nottingham, Queen s Medical Centre, Nottingham, UK
    Nucleic Acids Res 38:190-202. 2010
    ..Furthermore, this conformational change requires specific contacts between transposase and the flanking TA dinucleotide...
  19. pmc PiggyBac transposon-mediated, reversible gene transfer in human embryonic stem cells
    You Tzung Chen
    Graduate Institute of Clinical Genomics and Graduate Institute of Clinical Medicine, Stem Cell Core Laboratory, NTU Research Center for Medical Excellence, Taipei, Taiwan
    Stem Cells Dev 19:763-71. 2010
    ..We also demonstrate that the integrated piggyBac transposon can be removed and an undisrupted insertion site can be restored, which implies potential applications for its use in gene therapy and genetics studies...
  20. pmc Mechanism of IS200/IS605 family DNA transposases: activation and transposon-directed target site selection
    Orsolya Barabas
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 132:208-20. 2008
    The smallest known DNA transposases are those from the IS200/IS605 family. Here we show how the interplay of protein and DNA activates TnpA, the Helicobacter pylori IS608 transposase, for catalysis...
  21. pmc Structuring the bacterial genome: Y1-transposases associated with REP-BIME sequences
    Bao Ton-Hoang
    Laboratoire de Microbiologie et Genetique Moleculaires, Centre National de la Recherche Scientifique, 118, route de Narbonne, 31062 Toulouse Cedex, France
    Nucleic Acids Res 40:3596-609. 2012
    ..also resemble the sub-terminal hairpins of the atypical IS200/605 family of insertion sequences which encode Y1 transposases (TnpA(IS200/IS605))...
  22. ncbi Target DNA bending is an important specificity determinant in target site selection in Tn10 transposition
    Patrick A Pribil
    Department of Biochemistry, University of Western Ontario, N6A 5B7, London, Ont, Canada
    J Mol Biol 330:247-59. 2003
    ..We also show that Ca(2+) facilitates target capture by stabilizing transposase interactions with sequences immediately flanking the insertion site. Ca(2+) could, in theory, exert this effect by stabilizing bends in the target DNA...
  23. pmc Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition
    Andrew Adey
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Genome Biol 11:R119. 2010
    ..We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing...
  24. ncbi Messenger RNA as a source of transposase for sleeping beauty transposon-mediated correction of hereditary tyrosinemia type I
    Andrew Wilber
    The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 15:1280-7. 2007
    ....
  25. ncbi Stable integration and conditional expression of electroporated transgenes in chicken embryos
    Yuki Sato
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara, 630 0192, Japan
    Dev Biol 305:616-24. 2007
    ..Thus, the techniques proposed in this study provide a novel approach to study the mechanisms of late organogenesis, for which chickens are most suitable model animals...
  26. pmc A novel DNA binding and nuclease activity in domain III of Mu transposase: evidence for a catalytic region involved in donor cleavage
    Z Wu
    Department of Biochemistry, University of Western Ontario, London, Canada
    EMBO J 14:3835-43. 1995
    ..This proposal for active site assembly is in agreement with the recently proposed domain sharing model by Yang et al. (Yang, J.Y., Kim, K., Jayaram, M. and Harshey, R.M. [1995] EMBO J., 14, 2374-2384)...
  27. pmc Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition
    Akihiro Urasaki
    Division of Molecular and Developmental Biology, National Institute of Genetics, Japan
    Genetics 174:639-49. 2006
    ..Also, our study established a basis for further biochemical and molecular biological studies for understanding roles of the repetitive sequence in the subterminal region in transposition...
  28. ncbi Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system
    Adam J Dupuy
    National Cancer Institute, Center for Cancer Research, Frederick, Maryland 21702, USA
    Nature 436:221-6. 2005
    ..The uses of SB are also not restricted to the mouse and could potentially be used for forward genetic screens in any higher eukaryote in which transgenesis is possible...
  29. pmc DNA repair by the cryptic endonuclease activity of Mu transposase
    Wonyoung Choi
    Section of Molecular Genetics and Microbiology and Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    Proc Natl Acad Sci U S A 107:10014-9. 2010
    ..We hypothesize that ClpX constitutes part of a highly regulated mechanism that unmasks the cryptic nuclease activity of MuA specifically in the repair pathway...
  30. doi Pig transgenesis by Sleeping Beauty DNA transposition
    Jannik E Jakobsen
    Department of Human Genetics, University of Aarhus, Wilh Meyers Allé 1240, 8000, Aarhus C, Denmark
    Transgenic Res 20:533-45. 2011
    ....
  31. pmc A transposon and transposase system for human application
    Perry B Hackett
    Department of Genetics, Cell Biology, and Development, Center for Genome Engineering, Institute of Human Genetics, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 18:674-83. 2010
    ..This review examines the rationale and safety implications of application of the SB system to genetically modify T cells to be manufactured in compliance with current good manufacturing practice (cGMP) for phase I/II trials...
  32. ncbi Sustained FVIII expression and phenotypic correction of hemophilia A in neonatal mice using an endothelial-targeted sleeping beauty transposon
    Li Liu
    Department of Pharmacology and Therapeutics, College of Medicine, University of Florida, Gainesville, FL 32610, USA
    Mol Ther 13:1006-15. 2006
    ..These studies demonstrate the feasibility of using endothelial-targeted SB transposons for the treatment of hemophilia A...
  33. pmc A functional role for transposases in a large eukaryotic genome
    Mariusz Nowacki
    Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA
    Science 324:935-8. 2009
    ..transposase genes play key roles in this process of genome-wide DNA excision, which suggests that transposases function in large eukaryotic genomes containing thousands of active transposons...
  34. ncbi Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line
    J L Bessereau
    Department of Biology, University of Utah, Salt Lake City 84112-0840, USA
    Nature 413:70-4. 2001
    ..Fourth, these insertions can subsequently be remobilized to generate deletion and frameshift mutations by imperfect excision...
  35. pmc Chromosomal transposition of PiggyBac in mouse embryonic stem cells
    Wei Wang
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 105:9290-5. 2008
    ..The comprehensive information from this study should facilitate further exploration of the potential of PB and SB DNA transposons in mammalian genetics...
  36. ncbi Gene transfer into genomes of human cells by the sleeping beauty transposon system
    Aron M Geurts
    Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Mol Ther 8:108-17. 2003
    ..With the recently described improvements to the transposon itself, the SB system appears to be a potential gene-transfer tool for human gene therapy...
  37. pmc Efficient nonviral Sleeping Beauty transposon-based TCR gene transfer to peripheral blood lymphocytes confers antigen-specific antitumor reactivity
    P D Peng
    Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Gene Ther 16:1042-9. 2009
    ..These results demonstrate the promise of nonviral ex vivo genetic modification of autologous lymphocytes for the treatment of cancer and immunologic disease...
  38. pmc DNA recognition and the precleavage state during single-stranded DNA transposition in D. radiodurans
    Alison Burgess Hickman
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    EMBO J 29:3840-52. 2010
    Bacterial insertion sequences (ISs) from the IS200/IS605 family encode the smallest known DNA transposases and mobilize through single-stranded DNA transposition...
  39. pmc Duration of expression and activity of Sleeping Beauty transposase in mouse liver following hydrodynamic DNA delivery
    Jason B Bell
    Department of Genetics, University of Minnesota, Minneapolis, Minnesota, USA
    Mol Ther 18:1796-802. 2010
    ..Thus, within the limits of current technology, we show that SB transposons appear to be as stably integrated as their viral counterparts...
  40. ncbi An Arabidopsis hAT-like transposase is essential for plant development
    Paul Bundock
    Institute for Biology, Leiden University, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands
    Nature 436:282-4. 2005
    ..for normal plant growth; it shares several characteristics with the hAT (hobo, Activator, Tam3) family of transposases. DAYSLEEPER was isolated as a factor binding to a motif (Kubox1) present in the upstream region of the ..
  41. pmc Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery
    Melanie Galla
    Department of Experimental Hematology, Institute of Transplantation Immunology, Institute of Cell and Molecular Pathology, Hannover Medical School, Hannover, Germany
    Nucleic Acids Res 39:7147-60. 2011
    ..The potential cytotoxicity of transposases requires careful assessment, considering that residual integration events of transposase expression vectors ..
  42. ncbi Duplication of cyb-3 (cyclin B3) suppresses sterility in the absence of mdf-1/MAD1 spindle assembly checkpoint component in Caenorhabditis elegans
    Maja Tarailo-Graovac
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada
    Cell Cycle 9:4858-65. 2010
    ..Importantly, we show here, for the first time, that doubling the CYB-3 (Cyclin B3) dosage suppresses sterility in the absence of the essential spindle assembly checkpoint component MDF-1 without causing a delay in the onset of anaphase...
  43. ncbi ClpX and MuB interact with overlapping regions of Mu transposase: implications for control of the transposition pathway
    I Levchenko
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Genes Dev 11:1561-72. 1997
    ....
  44. doi Automatic classification within families of transposable elements: application to the mariner Family
    Jacques Deric Rouault
    Laboratoire Evolution, Genomes et Speciation, UPR9034, CNRS, 91198 Gif sur Yvette, France and Université Paris Sud 11, 91405 Orsay, France
    Gene 448:227-32. 2009
    ..The classification obtained here from DNA sequences of 935 items matches the phylogenies of the transposase. The rate of error from a posteriori re-assignment is relatively low...
  45. doi Sleeping beauty--a mouse model for all cancers?
    Viive M Howell
    Functional Genomics Laboratory, Kolling Institute of Medical Research, University of Sydney, E25, Level 9, Kolling Building, Royal North Shore Hospital, St Leonards, NSW 2065, Australia
    Cancer Lett 317:1-8. 2012
    ..This review follows the refinement of SB for different cancers and assesses its potential as a model for all cancers and a tool for cancer gene discovery...
  46. pmc DDE transposases: Structural similarity and diversity
    Irina V Nesmelova
    Department of Physics and Optical Science, University of North Carolina, Charlotte, 28223, United States
    Adv Drug Deliv Rev 62:1187-95. 2010
    ..This process requires a transposase enzyme. During transposition DD[E/D]-transposases undergo a series of conformational changes...
  47. pmc Phylogenetic and functional characterization of the hAT transposon superfamily
    Peter Arensburger
    Department of Entomology, University of California, Riverside, CA 92521 0001, USA
    Genetics 188:45-57. 2011
    ..We find that the recently discovered SPIN transposons of mammals are located within the family of Buster elements...
  48. pmc A simple topological filter in a eukaryotic transposon as a mechanism to suppress genome instability
    Corentin Claeys Bouuaert
    School of Biomedical Sciences, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2UH, United Kingdom
    Mol Cell Biol 31:317-27. 2011
    ..This provides constraints on the enzymatic mechanism of the double-strand breaks in mariner transposition, excluding the most prevalent of the current models...
  49. ncbi Four genes, two ends, and a res region are involved in transposition of Tn5053: a paradigm for a novel family of transposons carrying either a mer operon or an integron
    G Y Kholodii
    Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia
    Mol Microbiol 17:1189-200. 1995
    ..We suggest that the tni genes were involved in the dissemination of integrons...
  50. pmc Comparative genomic integration profiling of Sleeping Beauty transposons mobilized with high efficacy from integrase-defective lentiviral vectors in primary human cells
    Brian Moldt
    Department of Human Genetics, University of Aarhus, Aarhus, Denmark
    Mol Ther 19:1499-510. 2011
    ..Based on these properties, HIV-1/SB vectors may become valuable tools for genetic engineering and therapeutic gene transfer...
  51. ncbi Recent horizontal transfer of mellifera subfamily mariner transposons into insect lineages representing four different orders shows that selection acts only during horizontal transfer
    David J Lampe
    Department of Biological Sciences, Duquesne University
    Mol Biol Evol 20:554-62. 2003
    ....
  52. pmc The processing of repetitive extragenic palindromes: the structure of a repetitive extragenic palindrome bound to its associated nuclease
    Simon A J Messing
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Nucleic Acids Res 40:9964-79. 2012
    ..Although a new clade of putative transposases (RAYTs or TnpA(REP)) is often associated with an increase in these repeats, it is not clear how these proteins ..
  53. pmc Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps
    Jochen Trauner
    1Department of Biology, Developmental Biology, Friedrich Alexander University Erlangen, Erlangen, Germany
    BMC Biol 7:73. 2009
    ..Even so, there is a pressing need for forward genetic analysis to escape the bias inherent in candidate gene approaches...
  54. ncbi Transposition of hAT elements links transposable elements and V(D)J recombination
    Liqin Zhou
    Howard Hughes Medical Institute and Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    Nature 432:995-1001. 2004
    ..Transposable elements are classified by their transposition mechanisms and by the transposases that mediate their movement...
  55. ncbi Transcriptional activities of the Sleeping Beauty transposon and shielding its genetic cargo with insulators
    Oliver Walisko
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Mol Ther 16:359-69. 2008
    ....
  56. pmc A Sleeping Beauty mutagenesis screen reveals a tumor suppressor role for Ncoa2/Src-2 in liver cancer
    Kathryn A O'Donnell
    Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 109:E1377-86. 2012
    ..These findings reveal genes and pathways that functionally restrain MYC-mediated liver tumorigenesis and therefore may provide targets for cancer therapy...
  57. pmc Reconstitution of a functional IS608 single-strand transpososome: role of non-canonical base pairing
    Susu He
    Laboratoire de Microbiologie et Genetique Moleculaires, Centre National de Recherche Scientifique, Unité Mixte de Recherche 5100, 118 Rte de Narbonne, F31062 Toulouse Cedex, France
    Nucleic Acids Res 39:8503-12. 2011
    ....
  58. ncbi Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex
    S D Fugmann
    Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Mol Cell 5:97-107. 2000
    ..we have identified a region of RAG1 with possible structural similarities to the active site regions of transposases and retroviral integrases...
  59. pmc A conditional transposon-based insertional mutagenesis screen for genes associated with mouse hepatocellular carcinoma
    Vincent W Keng
    Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Nat Biotechnol 27:264-74. 2009
    ....
  60. pmc Targeted gene deletions in C. elegans using transposon excision
    Christian Frøkjaer-Jensen
    Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City, Utah, USA
    Nat Methods 7:451-3. 2010
    ..Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker...
  61. doi Cotranslational control of DNA transposition: a window of opportunity
    Guy Duval-Valentin
    Laboratoire de Microbiologie et Genetique Moleculaires, CNRS UMR5100, Campus Université Paul Sabatier, 118 route de Narbonne, F31062 Toulouse Cedex, France
    Mol Cell 44:989-96. 2011
    ..One IS regulatory phenomenon is a preference of their transposases (Tpases) for action on the element from which they are expressed (cis) rather than on other copies of the same ..
  62. doi Target site selection by the mariner-like element, Mos1
    Gwénaelle Crénès
    Universite Francois Rabelais de Tours, GICC, Parc de Grandmont, 37200 Tours, France
    Genetica 138:509-17. 2010
    ..Analysis of the physical properties of sequences obtained in vitro and in vivo do not help to explain Mos1 integration preferences, suggesting that other characteristics must be involved in Mos1 target choice...
  63. pmc Hyperactive piggyBac gene transfer in human cells and in vivo
    Joseph E Doherty
    Medical Scientist Training Program, Baylor College of Medicine, Houston, TX 77030, USA
    Hum Gene Ther 23:311-20. 2012
    ..We conclude that hyperactive piggyBac elements can increase gene transfer in human cells and in vivo and should enable improved gene delivery using the piggyBac transposon system in a variety of cell and gene-therapy applications...
  64. pmc Sleeping Beauty transposase modulates cell-cycle progression through interaction with Miz-1
    Oliver Walisko
    Max Delbruck Center for Molecular Medicine, D 13092 Berlin, Germany
    Proc Natl Acad Sci U S A 103:4062-7. 2006
    ..Because nonhomologous end-joining is required for efficient SB transposition, the transposase-induced G(1) slowdown is probably a selfish act on the transposon's part to maximize the chance for a successful transposition event...
  65. pmc Unique contacts direct high-priority recognition of the tetrameric Mu transposase-DNA complex by the AAA+ unfoldase ClpX
    Aliaa H Abdelhakim
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell 30:39-50. 2008
    ..We conclude that an extended set of potential enzyme contacts are exposed upon assembly of the tetramer and function as internal guides to recruit ClpX, thereby ensuring that the tetrameric complex is a high-priority substrate...
  66. doi The bacterial Tn9 chloramphenicol resistance gene: an attractive DNA segment for Mos1 mariner insertions
    Gwénaelle Crénès
    GICC, UMR CNRS 6239, Universite Francois Rabelais de Tours, UFR des Sciences et Techniques, Parc de Grandmont, 37200, Tours, France
    Mol Genet Genomics 281:315-28. 2009
    ..All TA dinucleotides that are preferred for integration were found within either TATA or TA x TA motifs. However, these motifs are not sufficient to constitute an attractive dinucleotide, since four TATA and TA x TA sites are cold spots...
  67. pmc Unexpectedly high copy number of random integration but low frequency of persistent expression of the Sleeping Beauty transposase after trans delivery in primary human T cells
    Xin Huang
    Pediatric Blood and Marrow Transplantation, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Hum Gene Ther 21:1577-90. 2010
    ..Our data suggest that SB delivery via plasmid in T cells should be carried out with caution because of unexpectedly high copy numbers of randomly integrated SB transposase...
  68. ncbi Transposon-free insertions for insect genetic engineering
    Tarig H Dafa'alla
    Oxitec Limited, 71 Milton Park, Oxford OX14 4RX, UK
    Nat Biotechnol 24:820-1. 2006
    ..We describe a method for post-integration elimination of all transposon sequences in the pest insect Medfly, Ceratitis capitata. The resulting insertions lack transposon sequences and are therefore impervious to transposase activity...
  69. pmc Efficient mammalian germline transgenesis by cis-enhanced Sleeping Beauty transposition
    Daniel F Carlson
    The Center for Genome Engineering, Minneapolis, MN, USA
    Transgenic Res 20:29-45. 2011
    ..Given its simplicity, versatility and high efficiency, TnT with enhanced Sleeping Beauty components represents a compelling non-viral approach to modifying the mammalian germline...
  70. pmc A transposon toolkit for gene transfer and mutagenesis in protozoan parasites
    Jeziel D Damasceno
    Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil
    Genetica 138:301-11. 2010
    ..The piggyBac and Tn5 transposons have also been shown to mobilize across Plasmodium spp. genomes circumventing the current limitations in the genetic manipulation of these organisms...
  71. pmc Resetting the site: redirecting integration of an insertion sequence in a predictable way
    Catherine Guynet
    Laboratoire de Microbiologie et Genetique Moleculaires, CNRS UMR5100, 118 Rte de Narbonne, F31062 Toulouse Cedex, France
    Mol Cell 34:612-9. 2009
    ..This suggested a way to redirect IS608 transposition to novel target sites. As we demonstrate here, we can now direct insertions in a predictable way into a variety of different chosen target sequences, both in vitro and in vivo...
  72. pmc Hermes transposon distribution and structure in Musca domestica
    Ramanand A Subramanian
    Center for Biosystems Research, University of Maryland Biotechnology Institute, Rockville, MD 20850, USA
    J Hered 100:473-80. 2009
    ..The simple sequence repeat found in hobo elements is a feature of this transposon that evolved since the divergence of hobo and Hermes...
  73. ncbi Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP/Cre recombinase system to distinguish transposition-specific expression
    Paul R Score
    Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 13:617-24. 2006
    ..The results also provide additional evidence for the effectiveness of the Sleeping Beauty transposon system as an in vivo DNA-mediated gene transfer strategy for achieving long-term expression...
  74. ncbi Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transfer
    Andrew Wilber
    The Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Stem Cells 25:2919-27. 2007
    ..We conclude that the SB transposon system provides an effective approach with several advantages for genetic manipulation and durable gene expression in human ES cells...
  75. ncbi Active site sharing and subterminal hairpin recognition in a new class of DNA transposases
    Donald R Ronning
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 20:143-54. 2005
    ..Protein sequences of IS605 transposases do not bear the hallmarks of other well-characterized transposases...
  76. ncbi Post-integration behavior of a Mos1 mariner gene vector in Aedes aegypti
    Raymond Wilson
    Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742 4450, USA
    Insect Biochem Mol Biol 33:853-63. 2003
    ..aegypti for applications requiring a very high degree of vector stability but will have limited use in the construction of genetic drive, enhancer trap, or transposon tagging systems in this species...
  77. pmc Chimeric recombinases with designed DNA sequence recognition
    Aram Akopian
    Institute of Biomedical and Life Sciences, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, Scotland
    Proc Natl Acad Sci U S A 100:8688-91. 2003
    ..Our results demonstrate the functional autonomy of the resolvase catalytic domain and open the way to creating "custom-built" recombinases that act at chosen natural target sequences...
  78. pmc Transposons for cancer gene discovery: Sleeping Beauty and beyond
    Lara S Collier
    The Department of Genetics, Cell Biology and Development, The Cancer Center, The University of Minnesota Twin Cities, Church St SE, Minneapolis, Minnesota 55455, USA
    Genome Biol 8:S15. 2007
    ..Here, we discuss the future of Sleeping Beauty for cancer genetic studies and the potential use of additional transposable elements for somatic mutagenesis...
  79. ncbi Multiple oligomerisation domains in the IS911 transposase: a leucine zipper motif is essential for activity
    L Haren
    Laboratoire de Microbiologie et Genetique Moleculaire du CNRS, Universite Paul Sabatier, 118 route de Narbonne, Toulouse, 31062, France
    J Mol Biol 283:29-41. 1998
    ..The two other regions are located further towards the C-terminal end of the protein, adjacent to the leucine zipper and in the region that carries the conserved catalytic DD(35)E motif...
  80. pmc Sleeping beauty transposition from nonintegrating lentivirus
    Conrad A Vink
    Institute of Child Health, University College London, UK
    Mol Ther 17:1197-204. 2009
    ..Importantly, integration site analysis revealed redirection toward a profile mimicking SB-plasmid integration and away from integration within transcriptionally active genes favored by integrase-proficient lentiviral vectors (ILVs)...
  81. pmc Hybrid lentivirus-transposon vectors with a random integration profile in human cells
    Nicklas H Staunstrup
    Department of Human Genetics, University of Aarhus, Denmark
    Mol Ther 17:1205-14. 2009
    ..The novel hybrid vector system combines the attractive features of efficient gene delivery by viral transduction and a safer genomic integration profile by DNA transposition...
  82. pmc High-level genomic integration, epigenetic changes, and expression of sleeping beauty transgene
    Jianhui Zhu
    Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Biochemistry 49:1507-21. 2010
    ..In addition, long-range activation of host genes must now be recognized as a potential consequence of an inserted transgene cassette containing enhancer elements...
  83. pmc Shielding of sleeping beauty DNA transposon-delivered transgene cassettes by heterologous insulators in early embryonal cells
    Trine Dalsgaard
    Department of Human Genetics, University of Aarhus, Aarhus, Denmark
    Mol Ther 17:121-30. 2009
    ..We believe that insulated SB-derived vectors will become useful tools in transposon-based transgenesis and therapeutic gene transfer...
  84. doi Translating Sleeping Beauty transposition into cellular therapies: victories and challenges
    Zsuzsanna Izsvák
    Max Delbrück Center for Molecular Medicine MDC, Berlin, Germany
    Bioessays 32:756-67. 2010
    ..The first clinical application of the SB system will help to validate the safety of this approach...
  85. doi In vitro recombination and inverted terminal repeat binding activities of the Mcmar1 transposase
    Sylvaine Renault
    Universite Francois Rabelais de Tours, GICC, CNRS, UMR 6239, UFR des Sciences and Techniques, Parc de Grandmont, 37200 Tours, France
    Biochemistry 49:3534-44. 2010
    ..This suggests that the assembly of the transposition complex is more complex than that of those involved in the mobility of the Mos1 and Himar1 mariner elements...
  86. pmc The catalytic domain of all eukaryotic cut-and-paste transposase superfamilies
    Yao Wu Yuan
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 108:7884-9. 2011
    ..g., hAT, P) based on sequence similarity of the element-encoded transposase. The transposases from several superfamilies possess a protein domain containing an acidic amino acid triad (DDE or DDD) that ..
  87. ncbi The N-terminus of Himar1 mariner transposase mediates multiple activities during transposition
    Matthew G Butler
    Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA
    Genetica 127:351-66. 2006
    ..we are beginning to understand the precise mechanism of transposition of these elements, the structure of their transposases are still poorly understood...
  88. ncbi DNA recognition sites activate MuA transposase to perform transposition of non-Mu DNA
    Ilana Goldhaber-Gordon
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 277:7694-702. 2002
    ..Multiple recognition sites help physically position MuA subunits in the transpososome plus each individual site allosterically activates transposase...
  89. ncbi The mariner transposons belonging to the irritans subfamily were maintained in chordate genomes by vertical transmission
    Ludivine Sinzelle
    Transgenèse et Génétique des Amphibiens, CNRS UMR 8080, IBAIC, Universite Paris Sud, Bâtiment 447, Orsay Cedex, F 91405, France
    J Mol Evol 62:53-65. 2006
    ..The phylogenetic analysis of the relationships between MLE transposases reveals that Xtmar1 is closely related to Hsmar2 and Bytmar1 and that together they form a second distinct ..
  90. ncbi Sequence and positional requirements for DNA sites in a mu transpososome
    Ilana Goldhaber-Gordon
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 277:7703-12. 2002
    ..This variability causes only local perturbations in the protein-DNA complex, as indicated by experiments in which altered and unaltered DNA substrates are paired...
  91. doi Liver-directed gene therapy using the sleeping beauty transposon system
    Lalitha R Belur
    Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, and Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA
    Methods Mol Biol 434:267-76. 2008
    ....
  92. pmc Analysis of the piggyBac transposase reveals a functional nuclear targeting signal in the 94 c-terminal residues
    James H Keith
    University of Notre Dame, Notre Dame, Indiana, USA
    BMC Mol Biol 9:72. 2008
    ..A PSORTII analysis of the TPase amino acid sequence predicts a bipartite nuclear localization signal (NLS) near the c-terminus, just upstream of a putative ZnF (ZnF)...
  93. ncbi Transposon-based interferon gamma gene transfer overcomes limitations of episomal plasmid for immunogene therapy of glioblastoma
    A Wu
    Department of Neurosurgery, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Cancer Gene Ther 14:550-60. 2007
    ..SB appears to improve the efficacy of cytokine gene therapy using nonviral vectors by enhancing the duration of transgene expression...
  94. pmc Identification and analysis of a hyperactive mutant form of Drosophila P-element transposase
    Eileen L Beall
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    Genetics 162:217-27. 2002
    ..These data suggest that the activity of P-element transposase may be regulated by phosphorylation and demonstrate that one mutation, S129A, results in hyperactive transposition...
  95. pmc Generation of an inducible and optimized piggyBac transposon system
    Juan Cadinanos
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
    Nucleic Acids Res 35:e87. 2007
    ..As the activity of PB is higher than that of SB11 and SB12, two hyperactive SB transposases, we have characterized and improved the PB system in mouse ES cells...
  96. doi The mariner Mos1 transposase produced in tobacco is active in vitro
    Xavier Thomas
    Universite Francois Rabelais de Tours, GICC, Parc de Grandmont, 37200 Tours, France
    Genetica 138:519-30. 2010
    ..Several hypotheses are proposed that could explain the inhibition of HMNtMOS1 activity in planta...
  97. doi Transposase-transposase interactions in MOS1 complexes: a biochemical approach
    Guillaume Carpentier
    Universite Francois Rabelais de Tours, GICC, CNRS, UMR 6239, UFR Sciences and Techniques, Parc Grandmont, 37200 Tours, France
    J Mol Biol 405:892-908. 2011
    b>Transposases are proteins that have assumed the mobility of class II transposable elements...
  98. doi Mariner Mos1 transposase optimization by rational mutagenesis
    Stephanie Germon
    GICC, Universite Francois Rabelais de Tours, 37200, Tours, France
    Genetica 137:265-76. 2009
    ..Even if host factors are not essential for transposition, our data demonstrate that the host machinery is essential in regulating MOS1 activity...
  99. doi Abundant transposases encoded by the metagenome of a hydrothermal chimney biofilm
    William J Brazelton
    School of Oceanography and Center for Astrobiology and Early Evolution, University of Washington, Seattle, WA 98195, USA
    ISME J 3:1420-4. 2009
    ..More than 8% of all metagenomic reads showed significant sequence similarity to transposases; all available metagenomic data sets from other environments contained at least an order of magnitude fewer ..
  100. pmc Transposition of ISHp608, member of an unusual family of bacterial insertion sequences
    Bao Ton-Hoang
    Laboratoire de Microbiologie et Genetique Moleculaires, CNRS UMR5100, Toulouse Cedex, France
    EMBO J 24:3325-38. 2005
    ..We also demonstrate that TnpA, which is less than half the length of other transposases, is responsible for this and for ISHp608 transposition...
  101. doi In vitro reconstitution of a single-stranded transposition mechanism of IS608
    Catherine Guynet
    Laboratoire de Microbiologie et Genetique Moleculaires, CNRS UMR5100, 118 Rte de Narbonne, F31062 Toulouse Cedex, France
    Mol Cell 29:302-12. 2008
    ..This single-strand transposition mode has important implications not only for dispersion of IS608 but also for the other members of this very large IS family...

Research Grants89

  1. BRUCELLA VACCINE FOR BIOTERRORISM
    Gary Splitter; Fiscal Year: 2004
    ..Included in these islands are ORFs encoding hypothetical proteins, transporters, transposases, and transcriptional regulators that most likely aid successful establishment of Brucella in human infections...
  2. GENE AMPLIFICATION BY A TRANSPOSITION APPARATUS
    Hiroshi Nakai; Fiscal Year: 1999
    ..the component that catalyzes strand transfer, performing a function similar to retroviral integrases and other transposases. However, the participation of the Mu A protein is not over after catalyzing strand transfer...
  3. Genetic transformation system for Chlamydia suis
    DANIEL ROCKEY; Fiscal Year: 2007
    ..the island encode plasmid mobilization and replication functions, tetracycline efflux pump, and candidate transposases. Experimental data in a surrogate system suggests that one of these transposases was responsible for the ..
  4. Plant Transposases and their MITEs
    Susan Wessler; Fiscal Year: 2007
    ..As such, knowledge of TE-mediated mechanisms of diversification are intimately involved in improving human health and understanding how plant and animal genomes evolve. ..
  5. A gene-trap screen for hearing and balance
    DONNA FEKETE; Fiscal Year: 2007
    ..Pseudotyped retroviral vectors or Tol2 transposases will be used to insert a gene-trap construct into the zebrafish germline...
  6. TRANSPOSASES IN ETOPOSIDE RESISTANCE
    Robert A Hromas; Fiscal Year: 2010
    ..In addition, the levels of Metnase could predict which patient will respond to etoposide. ..
  7. MECHANISTIC ANALYSIS OF DNA TRANSPOSITION BY PHAGE MU
    Tania Baker; Fiscal Year: 2001
    ..become clear that Mu transposase is a member of a structurally related family of proteins that includes many transposases and the retroviral integrases. These proteins catalyze recombination as multimeric complexes bound to DNA...
  8. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    ADRIENNE COTTRELL; Fiscal Year: 2007
    ..To ascertain if the transposases of the IS110/IS492 Family and Piv, the site-specific DNA recombinase, define a new family of DNA recombinases ..
  9. MUTATOR TRANSPORTABLE ELEMENTS OF MAIZE
    Virginia Walbot; Fiscal Year: 2005
    ..regulated timing of events and switch in transpositional outcome will be elucidated by analyzing proteins (MURA transposases and MURB helper proteins) encoded by the regulatory transposon MuDR...
  10. MOLECULAR ANALYSIS OF THE INSERTION ELEMENT IS903
    KEITH DERBYSHIRE; Fiscal Year: 2004
    ..In particular, mutant transposases will be sought that have altered binding specificity, increased transposition activity, and the ability to ..
  11. BACTERIOPHAGE MU DNA TRANSPOSITION
    Rasika Harshey; Fiscal Year: 1992
    ..We propose experiments that specifically address the post-transcriptional regulation of A- protein synthesis. We hope to uncover novel ways in which transposases limit their expression and thereby their mutagenic potential.
  12. Human DNA transposons: evolutionary history and genomic impact
    CEDRIC FESCHOTTE; Fiscal Year: 2007
    ..The second aim of this research is to assess the enzymatic activities of two transposases, MAR and PGBD3, which are potentially expressed in several human tissues and were identified as being among the ..
  13. Human DNA transposons: evolutionary history and genomic impact
    CEDRIC FESCHOTTE; Fiscal Year: 2010
    ..The second aim of this research is to assess the enzymatic activities of two transposases, MAR and PGBD3, which are potentially expressed in several human tissues and were identified as being among the ..
  14. MUTATOR TRANSPORTABLE ELEMENTS OF MAIZE
    Virginia Walbot; Fiscal Year: 2001
    ..In vitro biochemical studies will examine the DNA binding properties and enzymatic activities of the transposes encoded by MuDR to determine if different transposases are required for "cut and paste" and replicative transposition.
  15. Secretion systems for anti-malarial bacterial strains
    David Lampe; Fiscal Year: 2006
    ..agglomerans that secrete antimalarial proteins can block the transmission of malaria from mosquitoes to their hosts in a rodent malaria model system. Both Type I and Type II secretion systems will be developed and tested. ..
  16. Genetic methods using the Tol2 transposable element
    Koichi Kawakami; Fiscal Year: 2006
    ..abstract_text> ..
  17. Characterization of V(D)J cleavage and repair complexes
    PATRICK SWANSON; Fiscal Year: 2007
    ..abstract_text> ..
  18. Gustatory and olfactory receptors of Anopheles gambiae
    Hugh Robertson; Fiscal Year: 2006
    ..This work will also contribute to our overall understanding of the molecular basis of chemoreception in insects and other animals. ..
  19. MARINER TRANSPOSONS OF MAMMALS
    Hugh Robertson; Fiscal Year: 2001
    ..It will also reveal how occasionally particular copies of transposons can become integral parts of their host genetic complements. ..
  20. THE MOLECULAR BASIS OF HAEMOPHILUS DUCREYI PATHOGENESIS
    THOMAS KAWULA; Fiscal Year: 2001
    ..The role of the exported SOS in H. ducreyi survival and lesion development will be evaluated in the swine model of chancroid using both normal and neutropenic pigs. ..
  21. MOLECULAR ANALYSIS OF IS50/TN5 TRANSPOSITION
    William Reznikoff; Fiscal Year: 2003
    ..During the next grant period we will extend our genetic, biochemical and structural studies of Tn5 transposition. ..
  22. Structural Biology of a Serine DNA Recombinase
    PHOEBE RICE; Fiscal Year: 2007
    ..It will also help in the development of more versatile genetic tools. ..
  23. Molecular recognition studies of human PB1 bromodomains
    Martin Thompson; Fiscal Year: 2007
    ....
  24. Understanding DNA specificity in the IHF/HU family
    PHOEBE RICE; Fiscal Year: 2005
    ..Hypotheses based on comparing and contrasting such data for the 3 different proteins to be studied will be tested by site-directed mutagenesis and domain-swap experiments. ..
  25. Mechanisms of Immune Avoidance by Haemophilus ducreyi
    THOMAS KAWULA; Fiscal Year: 2007
    ..ducreyi re infection. An H. ducreyi outer membrane protein that is a target for bactericidal antibody has been identified. This protein will be tested for the ability to elicit a protective immune response to H. ducreyi. ..