penicillin binding proteins

Summary

Summary: Bacterial proteins that share the property of binding irreversibly to PENICILLINS and other ANTIBACTERIAL AGENTS derived from LACTAMS. The penicillin-binding proteins are primarily enzymes involved in CELL WALL biosynthesis including MURAMOYLPENTAPEPTIDE CARBOXYPEPTIDASE; PEPTIDE SYNTHASES; TRANSPEPTIDASES; and HEXOSYLTRANSFERASES.

Top Publications

  1. ncbi Penicillin binding proteins: key players in bacterial cell cycle and drug resistance processes
    Pauline Macheboeuf
    Institut de Biologie Structurale Jean Pierre Ebel CNRS CEA UJF, UMR 5075, Laboratoire des Proteines Membranaires, Grenoble, France
    FEMS Microbiol Rev 30:673-91. 2006
  2. doi The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis
    Eric Sauvage
    Centre d Ingénierie des Protéines, Institut de Physique B5a et Institut de Chimie B6a, University of Liege, Sart Tilman, Belgium
    FEMS Microbiol Rev 32:234-58. 2008
  3. doi Physiological functions of D-alanine carboxypeptidases in Escherichia coli
    Anindya S Ghosh
    Department of Biotechnology, Indian Institute of Technology, Kharagpur, District West Midnapore, West Bengal, Pin 721302, India
    Trends Microbiol 16:309-17. 2008
  4. pmc Sequences near the active site in chimeric penicillin binding proteins 5 and 6 affect uniform morphology of Escherichia coli
    Anindya S Ghosh
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 185:2178-86. 2003
  5. doi Penicillin-binding proteins and beta-lactam resistance
    André Zapun
    Laboratoire d Ingénierie des Macromolécules, Institut de Biologie Structurale Jean Pierre Ebel, UMR 5075 CNRS, CEA, Universite Joseph Fourier, Grenoble, France
    FEMS Microbiol Rev 32:361-85. 2008
  6. pmc BOCILLIN FL, a sensitive and commercially available reagent for detection of penicillin-binding proteins
    G Zhao
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285 0438, USA
    Antimicrob Agents Chemother 43:1124-8. 1999
  7. pmc Recruitment of a penicillin-binding protein gene from Neisseria flavescens during the emergence of penicillin resistance in Neisseria meningitidis
    B G Spratt
    Microbial Genetics Group, School of Biological Sciences, University of Sussex, Brighton, United Kingdom
    Proc Natl Acad Sci U S A 86:8988-92. 1989
  8. pmc Bacterial cell wall synthesis: new insights from localization studies
    Dirk Jan Scheffers
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
    Microbiol Mol Biol Rev 69:585-607. 2005
  9. ncbi Resistance to beta-lactam antibiotics
    K Poole
    Department of Microbiology and Immunology, Queen s University, K7L 3N6, Kingston, Ontario, Canada
    Cell Mol Life Sci 61:2200-23. 2004
  10. doi The different shapes of cocci
    André Zapun
    Laboratoire d Ingénierie des Macromolécules, Institut de Biologie Structurale CEA CNRS UJF UMR5075, Grenoble, France
    FEMS Microbiol Rev 32:345-60. 2008

Detail Information

Publications186 found, 100 shown here

  1. ncbi Penicillin binding proteins: key players in bacterial cell cycle and drug resistance processes
    Pauline Macheboeuf
    Institut de Biologie Structurale Jean Pierre Ebel CNRS CEA UJF, UMR 5075, Laboratoire des Proteines Membranaires, Grenoble, France
    FEMS Microbiol Rev 30:673-91. 2006
    ....
  2. doi The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis
    Eric Sauvage
    Centre d Ingénierie des Protéines, Institut de Physique B5a et Institut de Chimie B6a, University of Liege, Sart Tilman, Belgium
    FEMS Microbiol Rev 32:234-58. 2008
    ..An overview of the content in PBPs of some bacteria is provided with an emphasis on comparing the biochemical properties of homologous PBPs (orthologues) belonging to different bacteria...
  3. doi Physiological functions of D-alanine carboxypeptidases in Escherichia coli
    Anindya S Ghosh
    Department of Biotechnology, Indian Institute of Technology, Kharagpur, District West Midnapore, West Bengal, Pin 721302, India
    Trends Microbiol 16:309-17. 2008
    ....
  4. pmc Sequences near the active site in chimeric penicillin binding proteins 5 and 6 affect uniform morphology of Escherichia coli
    Anindya S Ghosh
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 185:2178-86. 2003
    ..coli PBP 5...
  5. doi Penicillin-binding proteins and beta-lactam resistance
    André Zapun
    Laboratoire d Ingénierie des Macromolécules, Institut de Biologie Structurale Jean Pierre Ebel, UMR 5075 CNRS, CEA, Universite Joseph Fourier, Grenoble, France
    FEMS Microbiol Rev 32:361-85. 2008
    ....
  6. pmc BOCILLIN FL, a sensitive and commercially available reagent for detection of penicillin-binding proteins
    G Zhao
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285 0438, USA
    Antimicrob Agents Chemother 43:1124-8. 1999
    ..Finally, this method also allowed us to compare relative affinities of a PBP2x protein for different beta-lactam antibiotics with the aid of fluorescence polarization technology and to monitor a PBP2x protein during purification...
  7. pmc Recruitment of a penicillin-binding protein gene from Neisseria flavescens during the emergence of penicillin resistance in Neisseria meningitidis
    B G Spratt
    Microbial Genetics Group, School of Biological Sciences, University of Sussex, Brighton, United Kingdom
    Proc Natl Acad Sci U S A 86:8988-92. 1989
    ..flavescens, or a very similar commensal Neisseria species, appears to have occurred independently during the development of altered penA genes in non-beta-lactamase-producing penicillin-resistant strains of N. gonorrhoeae...
  8. pmc Bacterial cell wall synthesis: new insights from localization studies
    Dirk Jan Scheffers
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
    Microbiol Mol Biol Rev 69:585-607. 2005
    ..We discuss a model in which, at least for a subset of PBPs, the presence of substrate is a major factor in determining PBP localization...
  9. ncbi Resistance to beta-lactam antibiotics
    K Poole
    Department of Microbiology and Immunology, Queen s University, K7L 3N6, Kingston, Ontario, Canada
    Cell Mol Life Sci 61:2200-23. 2004
    ....
  10. doi The different shapes of cocci
    André Zapun
    Laboratoire d Ingénierie des Macromolécules, Institut de Biologie Structurale CEA CNRS UJF UMR5075, Grenoble, France
    FEMS Microbiol Rev 32:345-60. 2008
    ..The present review aims to integrate older ultra-structural data with recent localization studies, in order to clarify the relation between the mechanisms of cell wall synthesis and the determination of cell shape in various cocci...
  11. pmc Escherichia coli mutants lacking all possible combinations of eight penicillin binding proteins: viability, characteristics, and implications for peptidoglycan synthesis
    S A Denome
    Department of Microbiology and Immunology, School of Medicine, University of North Dakota, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 181:3981-93. 1999
    The penicillin binding proteins (PBPs) synthesize and remodel peptidoglycan, the structural component of the bacterial cell wall. Much is known about the biochemistry of these proteins, but little is known about their biological roles...
  12. ncbi beta-lactam resistance in Streptococcus pneumoniae: penicillin-binding proteins and non-penicillin-binding proteins
    R Hakenbeck
    University of Kaiserslautern, Department of Microbiology, Kaiserslautern, Germany
    Mol Microbiol 33:673-8. 1999
    ....
  13. pmc Diversity of beta-lactam resistance-conferring amino acid substitutions in penicillin-binding protein 3 of Haemophilus influenzae
    Henri Dabernat
    Laboratoire de Microbiologie, Centre national de reference des Haemophilus influenzae, Toulouse, France
    Antimicrob Agents Chemother 46:2208-18. 2002
    ..The level of resistance remains low but opens the way to greater resistance in the future...
  14. ncbi Listeria monocytogenes EGD lacking penicillin-binding protein 5 (PBP5) produces a thicker cell wall
    Dorota Korsak
    Department of General Microbiology, Institute of Microbiology, Warsaw University, Warsaw, Poland
    FEMS Microbiol Lett 251:281-8. 2005
    ..A Listeria monocytogenes mutant lacking functional PBP5 was constructed. Cells of the mutant are viable, showing that the protein is dispensable for growth, but grow slower and have thickened cell walls...
  15. pmc Endopeptidase penicillin-binding proteins 4 and 7 play auxiliary roles in determining uniform morphology of Escherichia coli
    Bernadette M Meberg
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, ND 58202 9037, USA
    J Bacteriol 186:8326-36. 2004
    ....
  16. pmc FtsZ collaborates with penicillin binding proteins to generate bacterial cell shape in Escherichia coli
    Archana Varma
    Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202, USA
    J Bacteriol 186:6768-74. 2004
    ..Previously, we showed that Escherichia coli mutants lacking multiple penicillin binding proteins (PBPs) display extensive morphological diversity...
  17. pmc Production of penicillin by fungi growing on food products: identification of a complete penicillin gene cluster in Penicillium griseofulvum and a truncated cluster in Penicillium verrucosum
    Federico Laich
    Instituto de Biotecnología de León INBIOTEC Area de Microbiología, Facultad de Ciencias Biologicas y Ambientales, Universidad de Leon, Parque Cientifico de Leon, Avda del Real, No 1, 24006 Leon, Spain
    Appl Environ Microbiol 68:1211-9. 2002
    ..Antibacterial activities due to the production of secondary metabolites other than penicillin were observed in some fungi...
  18. pmc Roles of low-molecular-weight penicillin-binding proteins in Bacillus subtilis spore peptidoglycan synthesis and spore properties
    D L Popham
    Department of Biology, Virginia Tech, Blacksburg, Virginia 24061, USA
    J Bacteriol 181:126-32. 1999
    ..A model for the specific roles of DacB and DacF in spore peptidoglycan synthesis is proposed...
  19. pmc An acquired and a native penicillin-binding protein cooperate in building the cell wall of drug-resistant staphylococci
    M G Pinho
    Laboratory of Microbiology, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 98:10886-91. 2001
    ..aureus from an extra species source...
  20. ncbi Activation for catalysis of penicillin-binding protein 2a from methicillin-resistant Staphylococcus aureus by bacterial cell wall
    Cosimo Fuda
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA
    J Am Chem Soc 127:2056-7. 2005
    ....
  21. ncbi Disorganization of cell division of methicillin-resistant Staphylococcus aureus by a component of tea (Camellia sinensis): a study by electron microscopy
    J M Hamilton-Miller
    Department of Medical Microbiology, Royal Free, London, UK
    FEMS Microbiol Lett 176:463-9. 1999
    ..aureus grown in the presence of the active principle. The morphological changes observed were consistent with selective inhibition of penicillin-binding proteins...
  22. ncbi Penicillin-binding proteins of listeria monocytogenes--a re-evaluation
    Dorota Korsak
    Department of Bacterial Physiology, Institute of Microbiology, University of Warsaw, Poland
    Acta Microbiol Pol 51:5-12. 2002
    ..cells or membranes isolated from them were treated with [3H]penicillin to allow identification of the penicillin binding proteins (PBPs) located in the cytoplasmic membrane...
  23. pmc Contributions of PBP 5 and DD-carboxypeptidase penicillin binding proteins to maintenance of cell shape in Escherichia coli
    D E Nelson
    Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202 9037, USA
    J Bacteriol 183:3055-64. 2001
    Escherichia coli has 12 recognized penicillin binding proteins (PBPs), four of which (PBPs 4, 5, and 6 and DacD) have DD-carboxypeptidase activity...
  24. ncbi Structural aspects for evolution of beta-lactamases from penicillin-binding proteins
    Samy O Meroueh
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA
    J Am Chem Soc 125:9612-8. 2003
    ....
  25. pmc Affinities of beta-lactams for penicillin binding proteins of Chlamydia trachomatis and their antichlamydial activities
    C Storey
    Division of Microbiology and Antimicrobial Research Centre, University of Leeds, Leeds LS2 9JT, United Kingdom
    Antimicrob Agents Chemother 45:303-5. 2001
    Binding affinities of beta-lactam antibiotics for the three penicillin binding proteins (PBPs) from Chlamydia trachomatis were determined in vitro and compared with their antichlamydial activities...
  26. ncbi The basis for resistance to beta-lactam antibiotics by penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus
    Cosimo Fuda
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA
    J Biol Chem 279:40802-6. 2004
    ..The observed conformational changes are likely to be a hallmark for how this enzyme carries out its catalytic function in cross-linking the bacterial cell wall...
  27. pmc Amplification and disruption of the phenylacetyl-CoA ligase gene of Penicillium chrysogenum encoding an aryl-capping enzyme that supplies phenylacetic acid to the isopenicillin N-acyltransferase
    Mónica Lamas-Maceiras
    Area de Microbiologia, Facultad de Ciencias Biologicas y Ambientales, Universidad de Leon, Campus de Vegazana, S N, 24071 Leon, Spain
    Biochem J 395:147-55. 2006
    ..The peroxisomal co-localization of these two proteins indicates that the last two enzymes of the penicillin pathway form a peroxisomal functional complex...
  28. pmc Penicillin-binding proteins and induction of AmpC beta-lactamase
    C C Sanders
    Center for Research in Anti Infectives and Biotechnology, Department of Medical Microbiology, Creighton University School of Medicine, Omaha, Nebraska 68178, USA
    Antimicrob Agents Chemother 41:2013-5. 1997
    ..Loss of PBP 4 resulted in diminished inducibility. This suggests that if PBPs are involved in induction of AmpC beta-lactamase, there is probably a redundancy in function among the different PBPs...
  29. ncbi Identification of the novel penicillin biosynthesis gene aatB of Aspergillus nidulans and its putative evolutionary relationship to this fungal secondary metabolism gene cluster
    Petra Spröte
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, 07745 Jena, Germany
    Mol Microbiol 70:445-61. 2008
    ..Our findings suggest that aatB is a paralogue of aatA. They extend the model of evolution of the penicillin biosynthesis gene cluster by recruitment of a biosynthesis gene and its cis-regulatory sites upon gene duplication...
  30. pmc Molecular characterization of the acyl-coenzyme A:isopenicillin N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli
    M B Tobin
    Department of Molecular Genetics Research, Lilly Research Laboratories, Indianapolis, Indiana 46285
    J Bacteriol 172:5908-14. 1990
    ..Therefore, a single gene is responsible for converting IPN to penicillin G. The active form of the enzyme may result from processing of the 40-kDa monomeric precursor to a heterodimer containing subunits of 11 and 29 kDa...
  31. ncbi Interspecies recombinational events during the evolution of altered PBP 2x genes in penicillin-resistant clinical isolates of Streptococcus pneumoniae
    G Laible
    Max Planck Institut für Molekelare Genetik, Berlin, Germany
    Mol Microbiol 5:1993-2002. 1991
    ....
  32. ncbi The isopenicillin N acyltransferases of Aspergillus nidulans and Penicillium chrysogenum differ in their ability to maintain the 40-kDa alphabeta heterodimer in an undissociated form
    Francisco J Fernandez
    Area de Microbiologia, Facultad de Ciencias Biologicas y Ambientales, Universidad de Leon, Spain
    Eur J Biochem 270:1958-68. 2003
    ....
  33. ncbi Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins
    J F Martin
    Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of Leon, Spain
    Antonie Van Leeuwenhoek 65:227-43. 1994
    ..The levels of the cefG transcript were very low as compared to those of pcbAB, pcbC and cefEF.(ABSTRACT TRUNCATED AT 400 WORDS)..
  34. pmc Restoration of susceptibility of methicillin-resistant Staphylococcus aureus to beta-lactam antibiotics by acidic pH: role of penicillin-binding protein PBP 2a
    Sandrine Lemaire
    Unité de Pharmacologie Cellulaire et Moléculaire, Universite Catholique de Louvain, B 1200 Brussels, Belgium
    J Biol Chem 283:12769-76. 2008
    ....
  35. pmc Biochemistry and comparative genomics of SxxK superfamily acyltransferases offer a clue to the mycobacterial paradox: presence of penicillin-susceptible target proteins versus lack of efficiency of penicillin as therapeutic agent
    Colette Goffin
    Center for Protein Engineering, Institut de Chimie, University of Liege, B 4000 Sart Tilman, Liege, Belgium
    Microbiol Mol Biol Rev 66:702-38, table of contents. 2002
    ..Shifting the wall peptidoglycan from the (4-->3) type to the (3-->3) type could help Mycobacterium tuberculosis and Mycobacterium leprae survive by making them penicillin resistant...
  36. ncbi Laboratory characterization of methicillin-resistant Staphylococcus aureus in Canadian hospitals: results of 5 years of National Surveillance, 1995-1999
    Andrew E Simor
    Department of Microbiology, Sunnybrook and Women s College Health Sciences Centre, Toronto, Canada
    J Infect Dis 186:652-60. 2002
    ..This study confirms that the epidemiology of MRSA in Canada is evolving, but most isolates at this time appear to belong to one of a small number of epidemic clones...
  37. ncbi Participation of PBP 3 in the acquisition of dicloxacillin resistance in Listeria monocytogenes
    G O Gutkind
    Departamento de Microbiologia e Inmunologia, Facultad de Farmacia y Bioquimica, Buenos Aires, Argentina
    J Antimicrob Chemother 25:751-8. 1990
    ..PBP 2 could not be detected in one of the mutants. No decrease in affinity for the radioactive tracer or dicloxacillin was detected in any other PBP of the resistant mutants...
  38. ncbi Role of the 'cre/blr-tag' DNA sequence in regulation of gene expression by the Aeromonas hydrophila beta-lactamase regulator, BlrA
    Matthew B Avison
    Bristol Centre for Antimicrobial Research and Evaluation, Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK
    J Antimicrob Chemother 53:197-202. 2004
    ....
  39. pmc Reconstruction of Escherichia coli mrcA (PBP 1a) mutants lacking multiple combinations of penicillin binding proteins
    B M Meberg
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 183:6148-9. 2001
    ....
  40. ncbi Neisseria gonorrhoeae penicillin-binding protein 3 exhibits exceptionally high carboxypeptidase and beta-lactam binding activities
    Miglena E Stefanova
    Division of Pharmaceutical Sciences, University of Missouri Kansas City, Kansas City, Missouri 64110, USA
    Biochemistry 42:14614-25. 2003
    ..gonorrhoeae, although deletion of both PBP 3 and PBP 4, the other low-molecular-mass PBP in N. gonorrhoeae, resulted in a decreased growth rate and marked morphological abnormalities...
  41. ncbi Dispersed mode of Staphylococcus aureus cell wall synthesis in the absence of the division machinery
    Mariana G Pinho
    Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
    Mol Microbiol 50:871-81. 2003
    ..The results have implications for understanding the nature of S. aureus morphogenesis and for inhibitors of cell division proteins as drug targets...
  42. ncbi Contribution of beta-lactamase and PBP amino acid substitutions to amoxicillin/clavulanate resistance in beta-lactamase-positive, amoxicillin/clavulanate-resistant Haemophilus influenzae
    Vlatka Matic
    Department of Pathology, Hershey Medical Center, 500 University Drive, Hershey, PA 17033, USA
    J Antimicrob Chemother 52:1018-21. 2003
    ..We concluded that amoxicillin/clavulanate resistance in the two BLPACR strains was due to changes in PBP3. The possibility of the presence of an extended spectrum beta-lactamase was excluded in the BLPACR strains studied...
  43. pmc Contribution of penicillin-binding protein homologs to antibiotic resistance, cell morphology, and virulence of Listeria monocytogenes EGDe
    Caitriona M Guinane
    Department of Microbiology, University College Cork, Cork, Ireland
    Antimicrob Agents Chemother 50:2824-8. 2006
    ..These genes were found to contribute in various degrees to beta-lactam resistance, cell morphology, or the virulence potential of this organism...
  44. pmc Characterization of the bifunctional glycosyltransferase/acyltransferase penicillin-binding protein 4 of Listeria monocytogenes
    Joanna Zawadzka-Skomial
    Mohammed Terrak, Centre d Ingénierie des Protéines, Universite de Liege, Institut de Chimie, B6a, B 4000 Sart Tilman, Belgium
    J Bacteriol 188:1875-81. 2006
    ..monocytogenes EGD did not have any significant effect on growth rate, peptidoglycan composition, cell morphology, or sensitivity to beta-lactam antibiotics but did increase the resistance of the mutant to moenomycin...
  45. ncbi Recruitment of penicillin-binding protein PBP2 to the division site of Staphylococcus aureus is dependent on its transpeptidation substrates
    Mariana G Pinho
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Microbiol 55:799-807. 2005
    ..In methicillin-resistant S. aureus, addition of oxacillin does not result in delocalization of PBP2 indicating that acylated PBP2 can be maintained in place by functional PBP2A, the central element of this resistance mechanism...
  46. ncbi PBP3 inhibition elicits adaptive responses in Pseudomonas aeruginosa
    Jesus Blazquez
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Científicas CSIC C Darwin, 3, Campus de la Universidad Autónoma Cantoblanco, 28049 Madrid, Spain
    Mol Microbiol 62:84-99. 2006
    ..Thus, transcriptional changes demonstrated in this work are likely to be adaptively relevant to cells that survive...
  47. ncbi Analysis of the murein of a Listeria monocytogenes EGD mutant lacking functional penicillin binding protein 5 (PBP5)
    Dorota Korsak
    Department of General Microbiology, Faculty of Biology, Warsaw University, Poland
    Pol J Microbiol 54:339-42. 2005
    ....
  48. pmc The beta-lactam-resistance modifier (-)-epicatechin gallate alters the architecture of the cell wall of Staphylococcus aureus
    Paul D Stapleton
    Microbiology Group, School of Pharmacy, 29 39 Brunswick Square, London WC1N 1AX, UK
    Microbiology 153:2093-103. 2007
    ..Thus, ECg-mediated alterations to the physical nature of the bilayer will elicit structural changes to WTA that result in modulation of the cell-surface properties necessary to maintain the beta-lactam-resistant phenotype...
  49. ncbi Penicillin-binding protein 4 of Escherichia coli: molecular cloning of the dacB gene, controlled overexpression, and alterations in murein composition
    B Korat
    Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    Mol Microbiol 5:675-84. 1991
    ....
  50. ncbi Penicillin binding proteins, beta-lactams, and lactamases: offensives, attacks, and defensive countermeasures
    A L Koch
    Biology Department, Indiana University, Bloomington 47405 6801, USA
    Crit Rev Microbiol 26:205-20. 2000
    ..This led to the evolution of beta-lactamases and resistant penicillin binding proteins (PBPs)...
  51. ncbi Penicillin-binding proteins as resistance determinants in clinical isolates of Streptococcus pneumoniae
    P Reichmann
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    Microb Drug Resist 2:177-81. 1996
    ..In contrast, high-level cefotaxime resistance appeared to be due to a low affinity PBP2a, indicating that this PBP can also function as a resistance determinant...
  52. ncbi Penicillin susceptibility and molecular characteristics of clinical isolates of Streptococcus pneumoniae at the University of Malaya Medical Center, Kuala Lumpur, Malaysia
    Mohd Nasir Mohd Desa
    Department of Clinical Laboratory Science, Faculty of Medicine and Health Science, Universiti Putra Malaysia, Malaysia
    Int J Infect Dis 7:190-7. 2003
    ..To determine the prevalence of penicillin resistance and molecular characteristics of pneumococcal isolates at the University of Malaya Medical Center...
  53. ncbi Evaluation of different primers for detecting mecA gene by PCR in comparison with phenotypic methods for discrimination of methicillin-resistant Staphylococcus aureus
    Taweeporn Siripornmongcolchai
    Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
    Southeast Asian J Trop Med Public Health 33:758-63. 2002
    ..There was a good correlation among the mecA gene detection, ODD and OSS methods. The discrepancy of three isolates between PCR and phenotypic methods should be clarified for other resistant mechanisms...
  54. ncbi First report of a catalase-negative methicillin-resistant Staphalococcus aureus
    Xavier Bertrand
    Service d Hygiène Hospitalière et d Epidémiologie moléculaire CHU, Besançon 25030, France
    Diagn Microbiol Infect Dis 43:245-6. 2002
    ..The isolated strain had phenotypic and genotypic characteristics of the major epidemic clone spreading in our region with the exception that catalase activity could not be found...
  55. ncbi [Clonal diffusion and evolution of mecA and Tn554 polymorphisms in methicillin-resistant Staphylococcus aureus in Italy]
    F Campanile
    Dipartimento di Scienze Microbiologiche e Scienze Ginecologiche Sezione di Microbiologia, Universit degli Studi di Catania
    Infez Med 9:30-8. 2001
    ....
  56. ncbi Nasopharyngeal carriage of drug-resistant Streptococcus pneumoniae in children with acute otitis media evaluated by polymerase chain reaction-based genotyping of penicillin-binding proteins
    Muneki Hotomi
    Department of Otolaryngology Head and Neck Surgery, Wakayama Medical University, Japan
    Acta Otolaryngol 122:72-7. 2002
    ..Strains with mutations in the three PBP genes were often isolated from children < or = 2 years old. Evaluation of mutations in PBP genes using PCR will prove useful for studying the epidemiology of antibiotic resistance...
  57. ncbi [Comparison of routine sensitivity tests and mecA gene analysis for determination of methicillin resistance in Staphylococcus aureus isolates]
    Tuba Atay
    Dokuz Eylul Universitesi Tip Fakultesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Izmir
    Mikrobiyol Bul 36:133-40. 2002
    ..In conclusion, susceptibility tests, when performed according to NCCLS recommendations, are found to be reliable and decisive, for the detection of methicillin resistance of S. aureus...
  58. pmc Effects of amino acid alterations in penicillin-binding proteins (PBPs) 1a, 2b, and 2x on PBP affinities of penicillin, ampicillin, amoxicillin, cefditoren, cefuroxime, cefprozil, and cefaclor in 18 clinical isolates of penicillin-susceptible, -intermedia
    Kensuke Nagai
    Department of Pathology, Hershey Medical Center, Hershey, Pennsylvania, USA
    Antimicrob Agents Chemother 46:1273-80. 2002
    ..Overall, the MICs for and PBP affinities of the strains correlated with the changes found in the PBP active binding sites...
  59. ncbi [Comparison of different detection methods for methicillin resistance in Staphylococcus aureus]
    R Soloaga
    Hospital de Ninos Ricardo Gutierrez, Buenos Aires, Argentina
    Rev Argent Microbiol 34:52-6. 2002
    ..According to our results the best performance was obtained with MOCP. However, as a rapid method, CR gave acceptable sensitivity for clinical purposes...
  60. pmc In vitro and in vivo activities of a novel cephalosporin, BMS-247243, against methicillin-resistant and -susceptible staphylococci
    Joan C Fung-Tomc
    Department of Microbiology, Bristol Myers Squibb Co, Wallingford, Connecticut 06492, USA
    Antimicrob Agents Chemother 46:971-6. 2002
    ..In conclusion, BMS-247243 has in vitro and in vivo activities against methicillin-resistant staphylococci and thus may prove to be useful in the treatment of infections caused by these multidrug-resistant organisms...
  61. ncbi Survey of methicillin-resistant coagulase-negative staphylococci isolated from the fingers of nursing students
    Takashi Kitao
    Department of Medical Laboratory Technology, Ehime College of Health Science, 543 Takooda, Tobecho, Iyo gun, Ehime 791 2101, Japan
    J Infect Chemother 9:30-4. 2003
    ..The present results show that basic preventive measures for cross-infection should be considered in the future, using such genetic analysis methods, so that MRCNS may not cause hospital infection...
  62. ncbi Mueller-Hinton agar is superior to PDM blood agar for detection of methicillin-resistant Staphylococcus aureus
    T Monsen
    Department of Clinical Bacteriology, University Hospital of Umea, S 901 85 Umea, Sweden
    Clin Microbiol Infect 9:61-4. 2003
    ..05). Irrespective of the method of susceptibility testing evaluated, MH NaCl agar was superior to PDM blood agar for the detection of oxacillin resistance in mecA-containing S. aureus...
  63. pmc Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): a disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-screen latex agglutination test
    Annie Felten
    Service de Bactériologie Virologie Hygiène, Hopital Saint Louis, 75475 Paris Cedex 10, France
    J Clin Microbiol 40:2766-71. 2002
    ..Therefore, the cefoxitin and moxalactam disk diffusion methods were the best-performing tests for routine detection of all classes of MRSA...
  64. ncbi Detection of intrinsic oxacillin resistance in non-multiresistant, oxacillin-resistant Staphylococcus aureus (NORSA)
    Iain B Gosbell
    J Antimicrob Chemother 51:468-70. 2003
  65. ncbi [Comparison of methods used to detect methicillin resistance in staphylococci]
    Cigdem Kuzucu
    Inonu Universitesi Tip Fakultesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Malatya
    Mikrobiyol Bul 36:253-7. 2002
    ..aureus strains, but oxacillin agar screen revealed to be unsatisfactory for the detection of methicillin resistance in CNS...
  66. ncbi Physiological and molecular analysis of a mecA-negative Staphylococcus aureus clinical strain that expresses heterogeneous methicillin resistance
    Reiko Yoshida
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113, Japan
    J Antimicrob Chemother 51:247-55. 2003
    ..The strain was characteristically a non-producer of autolytic enzyme, though the phenotype, which was lost easily, was not directly correlated with methicillin resistance...
  67. pmc RWJ-54428 (MC-02,479), a new cephalosporin with high affinity for penicillin-binding proteins, including PBP 2a, and stability to staphylococcal beta-lactamases
    Francois Malouin
    Essential Therapeutics, Inc, Mountain View, California 94043, USA
    Antimicrob Agents Chemother 47:658-64. 2003
    ..These data are consistent with its excellent activity against beta-lactam-resistant gram-positive bacteria...
  68. ncbi Inducibility and potential role of MecA-gene-positive oxacillin-susceptible Staphylococcus aureus from colonized healthcare workers as a source for nosocomial infections
    G Kampf
    Institute of Hygiene, University Hospital Benjamin Franklin, Free University Berlin, Berlin, Germany
    J Hosp Infect 54:124-9. 2003
    ..It might be useful to screen methicillin-susceptible S. aureus isolates from HCWs for the mecA gene when recurrent infections with MRSA occur on a ward and a source cannot be found...
  69. pmc Rapid screening and identification of methicillin-resistant Staphylococcus aureus from clinical samples by selective-broth and real-time PCR assay
    Hong Fang
    Department of Clinical Bacteriology, Huddinge University Hospital, SE 141 86 Stockholm, Sweden
    J Clin Microbiol 41:2894-9. 2003
    ..aureus were also tested by this method, which was proved to be a valuable tool for MRSA identification in a routine microbiological laboratory...
  70. pmc mecA gene is widely disseminated in Staphylococcus aureus population
    C L C Wielders
    Eijkman Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Centre, 3584 CX Utrecht, The Netherlands
    J Clin Microbiol 40:3970-5. 2002
    ..This supports the hypothesis that horizontal transfer plays an important role in the dissemination of the mecA gene in the S. aureus population...
  71. pmc Staphylococcus aureus nasal carriage in the community: a survey from central Italy
    G Zanelli
    Clinica delle Malattie Infettive, Dipartimento di Biologia Molecolare, Universita di Siena, Italia
    Epidemiol Infect 129:417-20. 2002
    ..12%). The presence of MRSA in the community of our area still appears to be a rare event. Among methicillin-susceptible S. aureus (MSSA) isolates, a surprisingly high rate (18%) of resistance to rifampin was observed...
  72. pmc Genotypes of invasive pneumococcal isolates recently recovered from Italian patients
    Giordano Dicuonzo
    Dipartimento di Medicina di Laboratorio e Microbiologia, Università Campus Bio Medico, Via Emilio Longoni 47, 00155 Rome, Italy
    J Clin Microbiol 40:3660-5. 2002
    ..One PSSP isolate was a novel type 24F isolate related to the multiresistant clone France(9V)-3. This work reports new PNSP strains and new serotype-clone associations...
  73. pmc Detection of methicillin and mupirocin resistance in Staphylococcus aureus isolates using conventional and molecular methods: a descriptive study from a burns unit with high prevalence of MRSA
    P U Krishnan
    Department of Clinical Microbiology, University College London Hospitals and PHLS Collaborating Centre, Grafton Way, III Floor, London W1E 6DB, UK
    J Clin Pathol 55:745-8. 2002
    ..To compare conventional phenotypic methods for the detection of methicillin and mupirocin resistance in Staphylococcus aureus in routine laboratory practice with reference to an established molecular method...
  74. ncbi Genetic basis of neonatal methicillin-resistant Staphylococcus aureus in Japan
    Ken Kikuchi
    Department of Infectious Diseases, Tokyo Women s Medical University, Tokyo, Japan
    Pediatr Int 45:223-9. 2003
    ..The environment of NICU and infection control intervention should be improved and a new strategy for control like vaccination or probiotics is required...
  75. pmc Penicillin-binding protein 1A, 2B, and 2X alterations in Canadian isolates of penicillin-resistant Streptococcus pneumoniae
    Kimberly A Nichol
    Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Canada
    Antimicrob Agents Chemother 46:3261-4. 2002
    ..5 micro g/ml had PBP 1A alterations near the STMK and SRN motifs. Sequence analysis revealed identical PBP 1A, PBP 2B, and PBP 2X substitution patterns among all isolates for which penicillin MICs were > or =1 micro g/ml...
  76. ncbi TAK-599, a novel N-phosphono type prodrug of anti-MRSA cephalosporin T-91825: synthesis, physicochemical and pharmacological properties
    Tomoyasu Ishikawa
    Pharmaceutical Research Division, Takeda Chemical Industries, Ltd, 2 17 85 Jusohonmachi, Yodogawa ku, 532 8686, Osaka, Japan
    Bioorg Med Chem 11:2427-37. 2003
    ..These results show that 1 (TAK-599) is a highly promising parenteral cephalosporin targeted for MRSA infection...
  77. pmc Rapid detection of methicillin resistance in coagulase-negative Staphylococci with the VITEK 2 system
    Matthias A Horstkotte
    Institut fur Medizinische Mikrobiologie und Immunologie, Universitatsklinikum Hamburg Eppendorf, D 20246 Hamburg, Germany
    J Clin Microbiol 40:3291-5. 2002
    ..The results of our study encourage the use of the VITEK 2 system, which proved to be a highly reliable and rapid phenotypic method for the detection of methicillin resistance in CoNS...
  78. ncbi Penicillin resistant Streptococcus pneumoniae isolated from infected children in Athens, Greece: resistance patterns, serotypes and penicillin-binding protein 2B mutation characterization by PCR
    Stylianos Chatzipanagiotou
    Int J Antimicrob Agents 20:147-9. 2002
  79. pmc Rapid real-time PCR for determination of penicillin susceptibility in pneumococcal meningitis, including culture-negative cases
    Angela M Kearns
    Public Health Laboratory, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE, United Kingdom
    J Clin Microbiol 40:682-4. 2002
    ..pneumoniae was detected in all instances. Real-time PCR proved more sensitive than culture, microscopy, or antigen detection and provided susceptibility data even in culture-negative cases...
  80. ncbi Molecular characteristics of penicillin-binding protein genes of penicillin-nonsusceptible Streptococcus pneumoniae isolated in the Netherlands
    K Overweg
    Department of Pediatrics, Sophia Children's Hospital/Erasmus University Rotterdam, The Netherlands
    Microb Drug Resist 7:323-34. 2001
    ..Finally, novel mosaic structures were identified indicating horizontal exchange of pbp gene parts among penicillin-nonsusceptible pneumococci...
  81. pmc Unique variations of pbp2b sequences in penicillin-nonsusceptible Streptococcus pneumoniae isolates from Korea
    Jin Yang Baek
    Asian Pacific Research Foundation for Infectious Diseases, Seoul 135 710, Korea
    J Clin Microbiol 42:1746-50. 2004
    ....
  82. pmc Antibiotic susceptibility in relation to penicillin-binding protein genes and serotype distribution of Streptococcus pneumoniae strains responsible for meningitis in Japan, 1999 to 2002
    Kimiko Ubukata
    Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5 9 1 Shirokane, Minato ku, Japan
    Antimicrob Agents Chemother 48:1488-94. 2004
    ..9 and 56.0%, respectively). These findings suggest the need for early introduction of pneumococcal conjugate vaccines and continuous bacteriological surveillance for meningitis...
  83. pmc Genetic and molecular characterization of beta-lactamase-negative ampicillin-resistant Haemophilus influenzae with unusually high resistance to ampicillin
    Frank S Kaczmarek
    Department of Immunology and Infectious Disease, Pfizer Global Research and Development, Groton, Connecticut 06340, USA
    Antimicrob Agents Chemother 48:1630-9. 2004
    ..67 micro g/ml, typical for BLNAR strains. These studies illustrate that BLNAR strains with high ampicillin MICs exist that have combined resistance mechanisms in PBP3 and in the AcrAB efflux pump...
  84. ncbi Molecular characterization of penicillin non-susceptible Streptococcus pneumoniae in Christchurch, New Zealand
    David C Bean
    Centre for Infectious Disease, Barts and the London School of Medicine and Dentistry, Queen Mary College, University of London, London E1 2AD, UK
    J Antimicrob Chemother 54:122-9. 2004
    ..To determine the epidemiological relationship between non-invasive penicillin non-susceptible Streptococcus pneumoniae isolates collected in the Christchurch community between 1997 and 2001...
  85. pmc Complete sequences of six penicillin-binding protein genes from 40 Streptococcus pneumoniae clinical isolates collected in Japan
    Yumiko Sanbongi
    Pharmaceutical Research Department, Meiji Seika Kaisha Ltd, 760 Morooka cho, Kohoku Ku, Yokohama 222 8567, Japan
    Antimicrob Agents Chemother 48:2244-50. 2004
    ..pneumoniae resistance to beta-lactam antibiotics. Our findings also suggest that the Thr411Ala mutation in PBP 2A may be associated with beta-lactam resistance...
  86. ncbi Mecillinam has potent in vitro activity against Chlamydophila pneumoniae ATCC VR1310
    Julieanne M Bostock
    J Antimicrob Chemother 53:1112. 2004
  87. ncbi [Methicillin resistance detection in Staphylococcus aureus: comparison between conventional methods and MRSA-Screen latex agglutination technique]
    R Soloaga
    Maestría en Microbiología Clínica, Pontificia Universidad Católica Argentina, Buenos Aires
    Rev Argent Microbiol 36:36-40. 2004
    ..All methods presented high sensitivity and specificity, but MRSA-Screen latex had the advantage of giving a reliable result, equivalent to PCR, in only 15 minutes...
  88. pmc In vitro activities of ME1036 (CP5609), a novel parenteral carbapenem, against methicillin-resistant staphylococci
    Mizuyo Kurazono
    Pharmaceutical Research Center, Meiji Seika Kaisha Ltd, 760 Morooka cho, Kohoku Ku, Yokohama 222 8567, Japan
    Antimicrob Agents Chemother 48:2831-7. 2004
    ..In conclusion, ME1036 demonstrated a broad antibacterial spectrum and high levels of activity in vitro against staphylococci, including beta-lactam-resistant strains...
  89. ncbi Molecular epidemiology of penicillin-resistant Streptococcus pneumoniae in a university hospital, Ankara, Turkey
    A Pinar
    Hacettepe University, Faculty of Medicine, Department of Microbiology and Clinical Microbiology, Ankara, Turkey
    Clin Microbiol Infect 10:718-23. 2004
    ..pneumoniae and viridans group streptococci may have occurred...
  90. pmc Transcriptional analysis of the Staphylococcus aureus penicillin binding protein 2 gene
    M G Pinho
    Laboratory of Microbiology, The Rockefeller University, New York, New York 10021, USA
    J Bacteriol 180:6077-81. 1998
    ..We now show that introduction of the intact pbp2 gene with its two newly identified promoters into the chromosome of the transposon mutant resulted in the full recovery of high-level methicillin resistance...
  91. ncbi Cell wall-affecting antibiotics induce expression of a novel gene, drp35, in Staphylococcus aureus
    H Murakami
    College of Medical Technology and Nursing, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305, Japan
    Biochem Biophys Res Commun 264:348-51. 1999
    ..Homology search revealed that Drp35 was a new protein. Our results revealed that it was specific in S. aureus and respondent to these agents in both methicillin-resistant and -sensitive strains of S. aureus...
  92. pmc Staphylococcus aureus penicillin-binding protein 4 and intrinsic beta-lactam resistance
    U U Henze
    Institute of Medical Microbiology, University of Zurich, Switzerland
    Antimicrob Agents Chemother 39:2415-22. 1995
    ..The abcA-pbp4-tagD gene cluster was located in the SmaI-D fragment in the S. aureus 8325 chromosome in close proximity to the RNA polymerase gene rpoB...
  93. ncbi Vancomycin-intermediate Staphylococcus aureus with phenotypic susceptibility to methicillin in a patient with recurrent bacteremia
    Timothy S Naimi
    Acute Disease Epidemiology Section and Division of Public Health Laboratories, Minnesota Department of Health, Minneapolis, MN, USA
    Clin Infect Dis 36:1609-12. 2003
    ..All isolates were mecA positive and were indistinguishable by pulsed-field gel electrophoresis. The evolving susceptibility patterns of this strain highlight the challenges of detecting and treating VISA infections...
  94. pmc Point mutations in Staphylococcus aureus PBP 2 gene affect penicillin-binding kinetics and are associated with resistance
    C J Hackbarth
    Division of Infectious Diseases, San Francisco General Hospital, California 94110
    Antimicrob Agents Chemother 39:103-6. 1995
    ..These structural and biochemical changes may contribute to the strains' resistance to beta-lactam antibiotics...
  95. ncbi Effect of disruption of Staphylococcus aureus PBP4 gene on resistance to beta-lactam antibiotics
    Yuki Katayama
    Division of Infectious Diseases, San Francisco General Hospital, Department of Medicine, University of California San Francisco, San Francisco, CA 94110, USA
    Microb Drug Resist 9:329-36. 2003
    ..These data indicate that PBP 4 is a relatively unimportant target of beta-lactams not only in methicillin-susceptible but also methicillin-resistant S. aureus...
  96. ncbi Detection of mecA, mecR1 and mecI genes among clinical isolates of methicillin-resistant staphylococci by combined polymerase chain reactions
    E Petinaki
    Department of Microbiology, School of Medicine, University of Patras, 26500 Rion, Patras, Greece
    J Antimicrob Chemother 47:297-304. 2001
    ..Among methicillin-resistant S. aureus, a genetically heterogeneous population was identified, with several alterations and deletions of mec genes...
  97. ncbi A comparison of PCR detection of mecA with two standard methods of oxacillin disk susceptibility testing for coagulase-negative staphylococci
    Thean Y Tan
    Department of Medical Microbiology and Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff
    J Med Microbiol 51:83-5. 2002
    ..In this study, the clinical utility of mecA detection in CNS for the determination of methicillin resistance appears to be limited...
  98. pmc Differential expression of methicillin resistance by different biofilm-negative Staphylococcus epidermidis transposon mutant classes
    Dietrich Mack
    Institut fur Medizinische Mikrobiologie und Immunologie, Universitatsklinikum Hamburg Eppendorf, 20246 Hamburg, Federal Republic of Germany
    Antimicrob Agents Chemother 46:178-83. 2002
    ....
  99. ncbi Epidemiological aspects of antibiotic resistance in respiratory pathogens
    G Mlynarczyk
    Department of Medical Microbiology, Medical University of Warsaw, 5 Chalubinskiego, 02-004, Warsaw, Poland
    Int J Antimicrob Agents 18:497-502. 2001
    ..pneumoniae resistance is an effect of the changes in penicillin binding proteins. Among respiratory pathogens, resistance to tetracyclines, macrolides, trimethoprim-sulphamethoxazole ..
  100. pmc Rapid detection of methicillin resistance in coagulase-negative staphylococci by a penicillin-binding protein 2a-specific latex agglutination test
    M A Horstkotte
    , , D-20246 Hamburg, Germany
    J Clin Microbiol 39:3700-2. 2001
    ..Determination of oxacillin MICs evaluated according to the new breakpoint (0.5 microg/ml) of the National Committee for Clinical Laboratory Standards exhibited an extremely low specificity for this population...
  101. pmc Comparison of the Vitek gram-positive susceptibility 106 card, the MRSA-Screen latex agglutination test, and mecA analysis for detecting oxacillin resistance in a geographically diverse collection of clinical isolates of coagulase-negative staphylococci
    T Yamazumi
    Medical Microbiology Division, Department of Pathology University of Iowa College of Medicine, Iowa City, Iowa, USA
    J Clin Microbiol 39:3633-6. 2001
    ..Although the MRSA-Screen test required a slight procedural modification, both commercial methods achieved a sensitivity and specificity at detecting oxacillin resistance in CoNS at a level that was acceptable for clinical laboratory use...

Research Grants94

  1. Cell wall synthesis enzymes and beta-lactam resistance in Enterococcus faecium
    Louis B Rice; Fiscal Year: 2010
    ..This project aims to identify and characterize the complex and redundant mechanisms of ?-lactam resistance in an effort to better understand enterococcal physiology and promote identification of novel therapeutic targets. ..
  2. PENICILLIN RESISTANT NEISSERIA GONORRHOEAE (CMRNG)
    Robert Nicholas; Fiscal Year: 2000
    ..The three-dimensional structure of a lethal target of beta-lactam antibiotic also may lead to the rational design of new antibiotics based on molecular modeling of the beta- lactam binding site. ..
  3. B-lactam mediated SOS response and expression of resistance in clinical MRSA
    Adriana E Rosato; Fiscal Year: 2010
    ....
  4. STAPHYLOCOCCAL METHICILLIN RESISTANCE LOCUS
    Gordon Archer; Fiscal Year: 2007
    ..The purine biosynthetic operons will be genetically manipulated and correlated with development of VR and OR. In addition, microarray and proteomic studies will be pursued on other agents that perturb the cell wall. ..
  5. REGULATION OF AMPICILLIN RESISTANCE IN E FACEIUM
    Louis Rice; Fiscal Year: 2004
    ..They will also yield important new information on mechanisms of cell wall synthesis in E. faecium and other Gram-positive bacteria as well as on the mechanisms by which ampicillin resistance in E. faecium is regulated. ..
  6. Peptidoglycan Biogenesis in Escherichia Coli
    Thomas G Bernhardt; Fiscal Year: 2010
    ..Penicillin targets the PG synthases called the penicillin binding proteins (PBPs)...
  7. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2009
    ..This project will use X-ray crystallography to provide such information for a number of key proteins in N. gonorrhoeae that are involved in cell wall synthesis. ..
  8. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2010
    ..This project will use X-ray crystallography to provide such information for a number of key proteins in N. gonorrhoeae that are involved in cell wall synthesis. ..
  9. Penicillin-Binding Proteins, Mechanism and Inhibition
    Shahriar Mobashery; Fiscal Year: 2007
    ..It is expected that the outcome of these studies will shed definitive light on the biochemical mechanisms of PBPs and pave the way for discoveries of novel inhibitors as potential future antibiotics. ..
  10. Role of penicillin binding protein 1a in GBS virulence
    Amanda Jones; Fiscal Year: 2006
    ..abstract_text> ..
  11. Role of penicillin binding protein 1a in GBS virulence
    Craig Rubens; Fiscal Year: 2003
    ..abstract_text> ..
  12. Penicillin-resistant Neisseria gonorrhoeae (CMRNG)
    Robert Nicholas; Fiscal Year: 2005
    ..abstract_text> ..
  13. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2006
    ..These studies will provide a framework for future studies aimed at structure-based drug design and will provide substantial insight into the mechanisms of peptidoglycan synthesis. ..
  14. COMPLEX PHENOTYPES OF MUTIPLE MUTANTS OF E COLI
    Kevin Young; Fiscal Year: 2003
    ..The twelve penicillin binding proteins (PBPs) of Escherichia coli form a model system for the study of such "complex phenotypes...
  15. Mechanisms of antibiotic action in Neisseria gonorrhoeae.
    Robert A Nicholas; Fiscal Year: 2010
    ..Together, these projects utilize genetic, biochemical, biophysical, and proteomics approaches to generate new insights into the mechanisms of antibiotic action in N. gonorrhoeae. ..
  16. Peptidomimetics of D-Ala-D-Ala as Novel Antibacterials
    Norma Dunlap; Fiscal Year: 2003
    ..Penicillins are bacterial cell wall synthesis inhibitors and act by inhibition of Penicillin Binding Proteins (PBP's), also know as D-D-peptidases...
  17. BETA LACTAMASE MUTATIONS IN ANTIBIOTIC RESISTANCE
    Timothy Palzkill; Fiscal Year: 2007
    ..mutagenesis and phage display, to create derivatives of BLIP that bind and inhibit P-lactamases and penicillin binding proteins. The new BLIP derivatives will be characterized biochemically and structurally...
  18. NEW INHIBITORS FOR THE PENICILLIN BINDING PROTEINS
    WILLIAM GUTHEIL; Fiscal Year: 2002
    ..These studies will established improved methods for the study of the PBPs, determine essential features of PBP substrate specificity, and use this information to design new PBP inhibitors. ..
  19. Structure of Beta-lactam resistance regulators
    MARTIN SAFO; Fiscal Year: 2007
    ..Subsequently, the structures of the proteins will be determined using X-ray crystallography. ..
  20. STRUCTURE/FUNCTION OF ENGINEERED B LACTAMASES
    Osnat Herzberg; Fiscal Year: 2002
    ..This mutant and the native proteins will serve as the parent molecules for engineering a carboxypeptidase activity toward D-Ala-D-ala peptide, the natura substrate of the bacterial cell wall peptidases. ..
  21. Regulation/mecA gene expression/clinic S.aureus isolates
    Adriana Rosato; Fiscal Year: 2005
    ..Specifically, we will evaluate if the inducible overexpression of either MecR1/BlaR1 or their corresponding mutants/deletants in the absence of Beta-lactam induction results in a toxic effect to the cell. ..