monophenol monooxygenase

Summary

Summary: An enzyme of the oxidoreductase class that catalyzes the reaction between L-tyrosine, L-dopa, and oxygen to yield L-dopa, dopaquinone, and water. It is a copper protein that acts also on catechols, catalyzing some of the same reactions as CATECHOL OXIDASE. EC 1.14.18.1.

Top Publications

  1. ncbi Constituents from the Formosan apple reduce tyrosinase activity in human epidermal melanocytes
    Yi Pei Lin
    Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, 250 Wu Hsing Street, Taipei 110, Taiwan
    Phytochemistry 68:1189-99. 2007
  2. ncbi The proPO-system: pros and cons for its role in invertebrate immunity
    Lage Cerenius
    Department of Comparative Physiology, Uppsala University, 752 36 Uppsala, Sweden
    Trends Immunol 29:263-71. 2008
  3. ncbi Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future
    Y J Kim
    Center for Advanced Science and Innovation, Osaka University, Suita, Osaka, 565 0871, Japan
    Cell Mol Life Sci 62:1707-23. 2005
  4. ncbi A new 2-imino-1,3-thiazoline derivative, KHG22394, inhibits melanin synthesis in mouse B16 melanoma cells
    Dong Seok Kim
    Department of Dermatology, Seoul National University College of Medicine, Korea
    Biol Pharm Bull 30:180-3. 2007
  5. pmc An updated review of tyrosinase inhibitors
    Te Sheng Chang
    Department of Biological Science and Technology, National University of Tainan, Taiwan
    Int J Mol Sci 10:2440-75. 2009
  6. ncbi Role of the prophenoloxidase-activating system in invertebrate immunity
    K Soderhall
    Division of Physiological Mycology, University of Uppsala, Sweden
    Curr Opin Immunol 10:23-8. 1998
  7. ncbi Curcumin inhibits melanogenesis in human melanocytes
    Cai Xia Tu
    Department of Dermatology, 2nd Affiliated Hospital of Dalian Medical University, Liaoning 116027, PR China
    Phytother Res 26:174-9. 2012
  8. ncbi Dichloromethane fraction of Cimicifuga heracleifolia decreases the level of melanin synthesis by activating the ERK or AKT signaling pathway in B16F10 cells
    Ji Yeon Jang
    Department of Oriental Medicine, College of Oriental Medicine, Dongeui University, Busan, South Korea
    Exp Dermatol 18:232-7. 2009
  9. ncbi Genetic determinants of hair, eye and skin pigmentation in Europeans
    Patrick Sulem
    deCODE Genetics, Sturlugata 8, 101 Reykjavik, Iceland
    Nat Genet 39:1443-52. 2007
  10. ncbi Chemical and instrumental approaches to treat hyperpigmentation
    Stefania Briganti
    San Gallicano Dermatological Institute, Via Elio Chianesi, Rome, Italy
    Pigment Cell Res 16:101-10. 2003

Detail Information

Publications282 found, 100 shown here

  1. ncbi Constituents from the Formosan apple reduce tyrosinase activity in human epidermal melanocytes
    Yi Pei Lin
    Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, 250 Wu Hsing Street, Taipei 110, Taiwan
    Phytochemistry 68:1189-99. 2007
    ..Thus, 3-hydroxyphloretin (7) and catechol (9) were the most active constituents from the Formosan apple; they exhibited anti-oxidant and tyrosinase reducing activities, suggesting their possible use as cosmetic agents...
  2. ncbi The proPO-system: pros and cons for its role in invertebrate immunity
    Lage Cerenius
    Department of Comparative Physiology, Uppsala University, 752 36 Uppsala, Sweden
    Trends Immunol 29:263-71. 2008
    ....
  3. ncbi Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future
    Y J Kim
    Center for Advanced Science and Innovation, Osaka University, Suita, Osaka, 565 0871, Japan
    Cell Mol Life Sci 62:1707-23. 2005
    ..This article overviews the various inhibitors obtained from natural and synthetic sources with their industrial importance...
  4. ncbi A new 2-imino-1,3-thiazoline derivative, KHG22394, inhibits melanin synthesis in mouse B16 melanoma cells
    Dong Seok Kim
    Department of Dermatology, Seoul National University College of Medicine, Korea
    Biol Pharm Bull 30:180-3. 2007
    ..Our findings indicate that 2-imino-1,3-thiazoline derivatives are potential skin whitening agents...
  5. pmc An updated review of tyrosinase inhibitors
    Te Sheng Chang
    Department of Biological Science and Technology, National University of Tainan, Taiwan
    Int J Mol Sci 10:2440-75. 2009
    ..This article surveys tyrosinase inhibitors newly discovered from natural and synthetic sources. The inhibitory strength is compared with that of a standard inhibitor, kojic acid, and their inhibitory mechanisms are discussed...
  6. ncbi Role of the prophenoloxidase-activating system in invertebrate immunity
    K Soderhall
    Division of Physiological Mycology, University of Uppsala, Sweden
    Curr Opin Immunol 10:23-8. 1998
    ..Several components of this system recently have been isolated and their structure determined...
  7. ncbi Curcumin inhibits melanogenesis in human melanocytes
    Cai Xia Tu
    Department of Dermatology, 2nd Affiliated Hospital of Dalian Medical University, Liaoning 116027, PR China
    Phytother Res 26:174-9. 2012
    ..These results suggest that curcumin inhibits melanogenesis in human melanocytes through activation of Akt/GSK 3β, ERK or p38 MAPK signaling pathways...
  8. ncbi Dichloromethane fraction of Cimicifuga heracleifolia decreases the level of melanin synthesis by activating the ERK or AKT signaling pathway in B16F10 cells
    Ji Yeon Jang
    Department of Oriental Medicine, College of Oriental Medicine, Dongeui University, Busan, South Korea
    Exp Dermatol 18:232-7. 2009
    ..Therefore, C. heracleifolia would be a useful therapeutic agent for treating hyperpigmentation and an effective component in whitening and/or lightening cosmetics...
  9. ncbi Genetic determinants of hair, eye and skin pigmentation in Europeans
    Patrick Sulem
    deCODE Genetics, Sturlugata 8, 101 Reykjavik, Iceland
    Nat Genet 39:1443-52. 2007
    ..3 is associated with freckles. The fifth region provided refinements to a previously reported association in OCA2, and the sixth encompasses previously described variants in MC1R...
  10. ncbi Chemical and instrumental approaches to treat hyperpigmentation
    Stefania Briganti
    San Gallicano Dermatological Institute, Via Elio Chianesi, Rome, Italy
    Pigment Cell Res 16:101-10. 2003
    ....
  11. ncbi Glycogen synthase kinase 3beta is activated by cAMP and plays an active role in the regulation of melanogenesis
    Mehdi Khaled
    INSERM U385, Biologie et Physiopathologie de la Peau, IFR 50, 28 Avenue de Valombrose, 06107 Nice Cedex 2, France
    J Biol Chem 277:33690-7. 2002
    ....
  12. ncbi Comparative biochemistry of eumelanogenesis and the protective roles of phenoloxidase and melanin in insects
    Manickam Sugumaran
    Department of Biology, University of Massachusetts, Boston, USA
    Pigment Cell Res 15:2-9. 2002
    ..indicate that tyrosinase and phenoloxidase are distinctly different enzymes in spite of possessing both monophenol monooxygenase activity as well as o-diphenoloxidase activity...
  13. pmc Tyrosinase related protein 1 (TRP1) functions as a DHICA oxidase in melanin biosynthesis
    T Kobayashi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    EMBO J 13:5818-25. 1994
    ..This enzyme activity appears to be essential to the further metabolism of DHICA to a high molecular weight pigmented biopolymer...
  14. ncbi Crystallographic evidence that the dinuclear copper center of tyrosinase is flexible during catalysis
    Yasuyuki Matoba
    Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
    J Biol Chem 281:8981-90. 2006
    ..Our crystallographic study shows evidence that the tyrosinase active center formed by dinuclear coppers is flexible during catalysis...
  15. ncbi Hypopigmenting agents: an updated review on biological, chemical and clinical aspects
    Francisco Solano
    Department of Biochemistry and Molecular Biology B, University of Murcia, Murcia, Spain
    Pigment Cell Res 19:550-71. 2006
    ..Mammalian skin or at least keratinocytes/melanocytes co-cultures should be preferred rather than pure melanocyte cultures or soluble tyrosinase...
  16. ncbi (-)-N-Formylanonaine from Michelia alba as a human tyrosinase inhibitor and antioxidant
    Hui Min Wang
    Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, and the Department of Orthopaedics, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan, ROC
    Bioorg Med Chem 18:5241-7. 2010
    ..To our knowledge, this is the first study to reveal the bioactivities of (-)-N-formylanonaine from this plant species...
  17. ncbi Chromosomal structure of the human TYRP1 and TYRP2 loci and comparison of the tyrosinase-related protein gene family
    R A Sturm
    Centre for Molecular and Cellular Biology, University of Queensland, Australia
    Genomics 29:24-34. 1995
    ..The evidence also suggests that at least some of the introns within the TYR, TYRP1, and TYRP2 coding regions were gained after duplication and that intron slippage is unlikely to have occurred...
  18. pmc p38 regulates pigmentation via proteasomal degradation of tyrosinase
    Barbara Bellei
    Laboratory of Cutaneous Physiopathology, San Gallicano Dermatologic Institute, IRCCS, 00144 Rome, Italy
    J Biol Chem 285:7288-99. 2010
    ....
  19. ncbi Identification and characterization of mouse Rab32 by mRNA and protein expression analysis
    Karine A Cohen-Solal
    Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    Biochim Biophys Acta 1651:68-75. 2003
    ..Based on these data, we propose the inclusion of Rab32 to the so-called melanocyte/platelet family of Rab proteins...
  20. ncbi Antimelanogenic and antioxidant properties of gallic acid
    You Jung Kim
    Department of Cosmetology, Pusan Women s College, Busan, Korea
    Biol Pharm Bull 30:1052-5. 2007
    ..Based on these results, I propose that GA exerts antimelanogenic activity coupled with antioxidant properties by suppressing RS generation and maintaining a higher GSH/GSSG ratio...
  21. ncbi Fungal tyrosinases: new prospects in molecular characteristics, bioengineering and biotechnological applications
    S Halaouli
    UMR 1163 INRA Université de Provence de Biotechnologie des Champignons Filamenteux, IFR 86 de Biotechnologie Agro Industrielle de Marseille, Marseille Cedex, France
    J Appl Microbiol 100:219-32. 2006
    ..Next, their most promising applications in food, pharmaceutical and environmental fields are presented and the bioengineering approaches used for the development of tyrosinase-overproducing fungal strains are discussed...
  22. ncbi Involvement of p38 MAPK signaling pathway in the anti-melanogenic effect of San-bai-tang, a Chinese herbal formula, in B16 cells
    Yan Ye
    Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
    J Ethnopharmacol 132:533-5. 2010
    ..In our previous screening assays, SBT was identified as an effective tyrosinase inhibitor. In this study, we aim to investigate the anti-melanogenic effect and mechanisms of SBT in B16 cells...
  23. ncbi Stimulation of melanogenesis by scoparone in B16 melanoma cells
    Jeong Yeh Yang
    Department of Biochemistry, Dental School, Chonbuk National University, Jeonju 561 756, Korea
    Acta Pharmacol Sin 27:1467-73. 2006
    ..The effect of coumarin derivatives on melanogenesis was investigated in B16 murine melanoma cells...
  24. ncbi New insights into the active site structure and catalytic mechanism of tyrosinase and its related proteins
    Concepcion Olivares
    Department of Biochemistry, Molecular Biology and Immunology, School of Medicine, University of Murcia, Espinardo, Spain
    Pigment Cell Melanoma Res 22:750-60. 2009
    ..This review summarizes our current knowledge of tyrosinase and Tyrps structure and function and describes the catalytic mechanism of tyrosinase and Dct/Tyrp2, which are better characterized...
  25. pmc Different cis-acting elements are involved in the regulation of TRP1 and TRP2 promoter activities by cyclic AMP: pivotal role of M boxes (GTCATGTGCT) and of microphthalmia
    C Bertolotto
    INSERM U385, Biologie et Physiopathologie de la Peau, Faculte de Medecine, Nice, France
    Mol Cell Biol 18:694-702. 1998
    ..These convergent and compelling results disclose at least a part of the molecular mechanism involved in the regulation of melanogenic gene expression by cAMP and emphasize the pivotal role of microphthalmia in this process...
  26. pmc L-DOPA is an endogenous ligand for OA1
    Vanessa M Lopez
    Department of Ophthalmology and Vision Science, The University of Arizona, Tucson, Arizona, USA
    PLoS Biol 6:e236. 2008
    ..Our results suggest that the retinal consequences of albinism caused by changes in melanin synthetic machinery may be treated by L-DOPA supplementation...
  27. doi Molecular diagnosis of oculocutaneous albinism: new mutations in the OCA1-4 genes and practical aspects
    Caroline Rooryck
    Pigment Cell Melanoma Res 21:583-7. 2008
  28. ncbi Possible involvement of ERK 1/2 in UVA-induced melanogenesis in cultured normal human epidermal melanocytes
    H Yanase
    Technical Research Laboratory, Kurabo Industries Ltd, Osaka, Japan
    Pigment Cell Res 14:103-9. 2001
    ....
  29. ncbi Antityrosinase and antioxidant effects of ent-kaurane diterpenes from leaves of Broussonetia papyrifera
    Horng Huey Ko
    Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, Kaohsiung 807, Taiwan, Republic of China
    J Nat Prod 71:1930-3. 2008
    ..The mild inhibition of tyrosinase and significant inhibition of xanthine oxidase suggests that 1-3 could be useful ingredients in the development of skin-protecting cosmetics...
  30. ncbi Inhibition of UVA-mediated melanogenesis by ascorbic acid through modulation of antioxidant defense and nitric oxide system
    Uraiwan Panich
    Department of Pharmacology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
    Arch Pharm Res 34:811-20. 2011
    ..We report herein that AA can protect against UVA-dependent melanogenesis possibly through the improvement of antioxidant defense capacity and inhibition of NO production through down-regulation of eNOS and iNOS mRNA...
  31. ncbi Tyrosinase mutations associated with Siamese and Burmese patterns in the domestic cat (Felis catus)
    L A Lyons
    Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616, USA
    Anim Genet 36:119-26. 2005
    ..Because the same mutations were identified in different breeds with similar phenotypes, the mutations are likely to be identical by descent rather than multiple mutation events occurring at the same site...
  32. ncbi Inhibitory effects of Ramulus mori extracts on melanogenesis
    Kang Tae Lee
    Coreana Cosmetics Co, 204 1 JeongchonRi, Senggeoeup, Cheonansi, 330 830, Korea
    J Cosmet Sci 54:133-42. 2003
    ..23 microg/ml). Also, R. mori extracts inhibited tyrosinase activity in a competitive manner (Ki = 1.5 x 10(-6) M) when L-tyrosine was used as a substrate...
  33. ncbi Similar enzyme activation and catalysis in hemocyanins and tyrosinases
    Heinz Decker
    Institut für Molekulare Biophysik, Johannes Gutenberg Universitat, 55099 Mainz, Germany
    Gene 398:183-91. 2007
    ..Minor conformational differences at the active site seem to decide about whether the active site is only able to oxidize diphenols as in catecholoxidase or if it is also able to o-hydroxylate monophenols as in tyrosinase...
  34. ncbi Comparison of the characteristics of fungal and plant tyrosinases
    Emilia Selinheimo
    VTT Technical Research Centre of Finland, P O Box 1000, Espoo FIN 02044 VTT, Finland
    J Biotechnol 130:471-80. 2007
    ..In the reaction conditions studied, TrT showed the best ability to crosslink alpha-casein. TrT also had the highest activity on most of the tested monophenols, and showed noticeable short lag periods prior to the oxidation...
  35. ncbi Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance
    Li Li
    Department of Plant Breeding and Biometry, 252 Emerson Hall, Cornell University, Ithaca, NY 14853 2901, USA Fax 1 607 2551132
    Planta 215:239-47. 2002
    ..These results demonstrate the importance of PPO-mediated phenolic oxidation in restricting plant disease development...
  36. ncbi Temporal patterns in immune responses to a range of microbial insults (Tenebrio molitor)
    Eleanor R Haine
    Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield, S10 2TN, UK
    J Insect Physiol 54:1090-7. 2008
    ..There was no long-lasting upregulation of phenoloxidase activity, suggesting that this immune effector system is not involved in the management of microbial infections over a long time scale...
  37. ncbi Flavonoids, taxifolin and luteolin attenuate cellular melanogenesis despite increasing tyrosinase protein levels
    Sang Mi An
    Department of Molecular Medicine, Kyungpook National University School of Medicine, 101 Dongindong 2 ga, Junggu, Daegu, 700 422, Republic of Korea
    Phytother Res 22:1200-7. 2008
    ..Therefore, the antimelanogenic effects of taxifolin and luteolin are attributed to their inhibitory effects on tyrosinase enzymatic activity, despite their effects on increasing tyrosinase protein levels...
  38. pmc Biphasic expression of two paracrine melanogenic cytokines, stem cell factor and endothelin-1, in ultraviolet B-induced human melanogenesis
    Akira Hachiya
    Kao Biological Science Laboratories, Ichikai machi Haga, Tochigi 321 3497 Japan
    Am J Pathol 165:2099-109. 2004
    ....
  39. ncbi Tyrosinase stabilization by Tyrp1 (the brown locus protein)
    T Kobayashi
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda Maryland 20892, USA
    J Biol Chem 273:31801-5. 1998
    ..In sum, these results suggest that, in addition to its catalytic function in oxidizing DHICA, Tyrp1 may play an important role in stabilizing Tyr, a second potential role in the regulation of melanin formation...
  40. ncbi Evaluation of anti-pigmentary effect of synthetic sulfonylamino chalcone
    Woo Duck Seo
    Department of Functional Crop, NICS, RDA, Miryang 627 803, Republic of Korea
    Eur J Med Chem 45:2010-7. 2010
    ..The Western blot for expression levels of proteins involved in melanogenesis showed that TSAHC significantly decreased three main tyrosinase related protein in melanin biosynthesis, tyrosinase, TRP-1 and TRP-2...
  41. ncbi Melasma: a comprehensive update: part II
    Vaneeta M Sheth
    Department of Dermatology at Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    J Am Acad Dermatol 65:699-714; quiz 715. 2011
    ..A thorough understanding of the risks and benefits of various therapeutic options is crucial in selecting the best treatment...
  42. ncbi Partially purified components of Nardostachys chinensis suppress melanin synthesis through ERK and Akt signaling pathway with cAMP down-regulation in B16F10 cells
    Ji Yeon Jang
    Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University, Gyeongnam, Republic of Korea
    J Ethnopharmacol 137:1207-14. 2011
    ..We investigated the inhibitory activities of Nardostachys chinensis in melanogenesis and its related signaling pathway...
  43. ncbi DNA-based eye colour prediction across Europe with the IrisPlex system
    Susan Walsh
    Department of Forensic Molecular Biology, Erasmus MC University Medical Centre Rotterdam, Rotterdam, The Netherlands
    Forensic Sci Int Genet 6:330-40. 2012
    ....
  44. ncbi Tyrosinase and related proteins in mammalian pigmentation
    V del Marmol
    LOCE, Institut J Bordet, Universite Libre de Bruxelles, Belgium
    FEBS Lett 381:165-8. 1996
    ..We will discuss recent findings on genomic organization, and on the proteins and their presumed function, which is important for eumelanin synthesis in mouse and man...
  45. pmc New insights into the evolution of metazoan tyrosinase gene family
    Rosaria Esposito
    Cellular and Developmental Biology Department, Stazione Zoologica Anton Dohrn, Villa Comunale, Napoli, Italy
    PLoS ONE 7:e35731. 2012
    ..Collectively, the results reported here enlarge our knowledge about the tyrosinase gene family as valuable resource for understanding the genetic components involved in pigment cells evolution and development...
  46. ncbi Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes
    H Inagaki
    Department of Biology, Faculty of Science, Nagoya University, Japan
    Gene 150:319-24. 1994
    ..In the b mutant, contrary to expectation, the mRNA was detected not only in eyes but also in amelanotic skin. Therefore, pigmentation of the skin controlled by b is not directly related to expression of the Tyr gene...
  47. ncbi Role of the C-terminal extension in a bacterial tyrosinase
    Michael Fairhead
    Empa, Swiss Federal Laboratories for Materials Testing and Research, Laboratory for Biomaterials, St Gallen, Switzerland
    FEBS J 277:2083-95. 2010
    ..In addition, we show that this enzyme can be readily overproduced and purified and that it will prove useful in furthering the understanding of these enzymes, as well as their biotechnological application...
  48. ncbi Antioxidant activity of some plant extracts towards xanthine oxidase, lipoxygenase and tyrosinase
    Chin Hui Chen
    College of Pharmacy, Taipei Medical University, 250 Wu Xin Street, Taipei 11031, Taiwan
    Molecules 14:2947-58. 2009
    ..Additional studies are warranted to further characterize the compounds responsible for the antioxidant properties of the examined extracts...
  49. ncbi Expression hierarchy of T cell epitopes from melanoma differentiation antigens: unexpected high level presentation of tyrosinase-HLA-A2 Complexes revealed by peptide-specific, MHC-restricted, TCR-like antibodies
    Yael Michaeli
    Faculty of Biology, Technion Israel Institute of Technology, Haifa, Israel
    J Immunol 182:6328-41. 2009
    ..These results may shed new light on relationships between Ag presentation and immune response to cancer Ags...
  50. ncbi Inhibition of melanogenesis by tyrosinase siRNA in human melanocytes
    Sang Mi An
    Department of Molecular Medicine and Cell and Matrix Research Institute, BK21 Medical Education Program for Human Resources, Kyungpook National University School of Medicine, Daegu, Korea
    BMB Rep 42:178-83. 2009
    ..These results suggest that molecular approaches using siRNA targeted to the enzymes of melanogenic pathway may provide a novel strategy for the control of cell pigmentation...
  51. ncbi Resveratrol as a kcat type inhibitor for tyrosinase: potentiated melanogenesis inhibitor
    Hiroki Satooka
    Department of Nutritional Science and Toxicology, University of California, Berkeley, CA 94720, United States
    Bioorg Med Chem 20:1090-9. 2012
    ..trans-Pinosylvin, cis-pinosylvin, dihydropinosylvin were also tested for a comparison. These results suggest that possible usage of resveratrol as a tyrosinase inhibitor and a melanogenesis inhibitor...
  52. ncbi The Tyr (albino) locus of the laboratory mouse
    Friedrich Beermann
    ISREC, National Center of Competence in Research NCCR Molecular Oncology, Chemin des Boveresses 155, 1066 Epalinges, Switzerland
    Mamm Genome 15:749-58. 2004
    ..We compare eye and skin pigmentation phenotypes and the genetic lesions that cause each. We suggest that this panel of congenic mutants contains rich, untapped resources for the study of many questions of basic cell biological interest...
  53. ncbi Selection for cuticular melanism reveals immune function and life-history trade-offs in Spodoptera littoralis
    S C Cotter
    Department of Biological Sciences, Lancaster Environment Centre, Lancaster University, Lancaster, UK
    J Evol Biol 21:1744-54. 2008
    ..In addition, we found that lines with high PO activity had slower development rates suggesting that investment in PO, rather than in melanism, is costly...
  54. ncbi BMP-2 stimulates tyrosinase gene expression and melanogenesis in differentiated melanocytes
    M L Bilodeau
    Basic Medical Sciences Department, Purdue University, West Lafayette, Indiana 47906, USA
    Pigment Cell Res 14:328-36. 2001
    ..These studies support a role for BMP-2 in melanogenesis by selectively targeting the expression of the tyrosinase gene involved in melanin biosynthesis...
  55. ncbi Potent virucidal activity in larval Heliothis virescens plasma against Helicoverpa zea single capsid nucleopolyhedrovirus
    Holly J R Popham
    USDA, Agricultural Research Service, Biological Control of Insects Research Laboratory, 1503 S Providence Road, Columbia, MO 65203, USA
    J Gen Virol 85:2255-61. 2004
    ..Thus, in the plasma of larval lepidopterans, the enzyme phenol oxidase may act as a constitutive, humoral innate antiviral immune response...
  56. ncbi Catecholamine synthesis is mediated by tyrosinase in the absence of tyrosine hydroxylase
    M Rios
    Departments of Neuroscience and Anatomy and Cell Biology, Tufts University Medical School, Boston, Massachusetts 02111, USA
    J Neurosci 19:3519-26. 1999
    ..Thus, these extraordinary circumstances reveal that tyrosinase serves as an alternative pathway to supply catecholamines...
  57. ncbi Functional analysis of microphthalmia-associated transcription factor in pigment cell-specific transcription of the human tyrosinase family genes
    K Yasumoto
    Department of Applied Physiology and Molecular Biology, Tohoku University School of Medicine, Aoba ku, Sendai, Japan
    J Biol Chem 272:503-9. 1997
    ..These results suggest that MITF is sufficient to direct pigment cell-specific transcription of the tyrosinase and TRP-1 genes but not the TRP-2 gene...
  58. ncbi Tyrosinase/catecholoxidase activity of hemocyanins: structural basis and molecular mechanism
    H Decker
    Institut für Molekulare Biophysik, Johannes Gutenberg Universitat Mainz, D 55099 Mainz, Germany
    Trends Biochem Sci 25:392-7. 2000
    ..On the basis of their molecular structures, hemocyanins are used as model systems to understand the substrate-active-site interaction between catecholoxidases and tyrosinases...
  59. pmc Model-based prediction of human hair color using DNA variants
    Wojciech Branicki
    Section of Forensic Genetics, Institute of Forensic Research, Westerplatte 9, 31 033, Krakow, Poland
    Hum Genet 129:443-54. 2011
    ....
  60. pmc Evolution of pigment synthesis pathways by gene and genome duplication in fish
    Ingo Braasch
    University of Wurzburg, Physiological Chemistry I, Biozentrum, Am Hubland, Wurzburg, Germany
    BMC Evol Biol 7:74. 2007
    ..No systematic genomic inventory allowing to test this hypothesis has been drawn up so far for pigmentation genes in fish, and almost nothing is known about the evolution of these genes in different fish lineages...
  61. pmc Melanosome maturation defect in Rab38-deficient retinal pigment epithelium results in instability of immature melanosomes during transient melanogenesis
    Vanda S Lopes
    Molecular and Cellular Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London SW7 2AZ, United Kingdom
    Mol Biol Cell 18:3914-27. 2007
    ..Together with the high levels of cathepsin D in immature melanosomes of the RPE, our results suggest that melanin deposition may protect the maturing melanosome from the activity of lumenal acid hydrolases...
  62. pmc Spatiotemporal features of early neuronogenesis differ in wild-type and albino mouse retina
    Rivka A Rachel
    Center for Neurobiology and Behavior, Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Neurosci 22:4249-63. 2002
    ..These results point to spatiotemporal defects in neuronal production in the albino retina, which could perturb expression of genes that specify cell fate, number, and/or projection phenotype...
  63. pmc Endoplasmic reticulum retention is a common defect associated with tyrosinase-negative albinism
    R Halaban
    Department of Dermatology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 97:5889-94. 2000
    ..From these results we concluded that albinism, at least in part, is an ER retention disease...
  64. ncbi Inhibition of the phosphatidylinositol 3-kinase/p70(S6)-kinase pathway induces B16 melanoma cell differentiation
    R Busca
    INSERM U385, Faculte de Medecine, 06107 Nice, Cedex 02, France
    J Biol Chem 271:31824-30. 1996
    ..Furthermore, we provide evidence which suggests that cAMP-induced melanogenesis and dendricity are, at least partially, mediated by the cAMP inhibition of the phosphatidylinositol 3-kinase/p70(S6)-kinase signaling pathway...
  65. pmc Broad-spectrum antimicrobial activity of the reactive compounds generated in vitro by Manduca sexta phenoloxidase
    Picheng Zhao
    Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078, USA
    Insect Biochem Mol Biol 37:952-9. 2007
    ..g. proteinases, oxidases, and dopachrome conversion enzyme (DCE)), which immobilizes and kills invading microorganisms...
  66. ncbi Phenoloxidase is an important component of the defense against Aeromonas hydrophila Infection in a crustacean, Pacifastacus leniusculus
    Haipeng Liu
    Department of Comparative Physiology, Uppsala University, Norbyvagen 18A, SE 752 36 Uppsala, Sweden
    J Biol Chem 282:33593-8. 2007
    ..Our data therefore suggest that phenoloxidase is required in crayfish defense against an infection by A. hydrophila, a highly virulent and pathogenic bacterium to crayfish...
  67. pmc A second tyrosinase-related protein, TRP-2, is a melanogenic enzyme termed DOPAchrome tautomerase
    K Tsukamoto
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    EMBO J 11:519-26. 1992
    ..We now report that TRP-2 (tyrosinase related protein-2), which maps to and is mutated at the slaty locus in mice, encodes a protein with DOPAchrome tautomerase activity...
  68. ncbi Bacterial tyrosinases
    Harald Claus
    Institute for Microbiology and Wine Research, University of Mainz, Becherweg 15, D 55099 Mainz, Germany
    Syst Appl Microbiol 29:3-14. 2006
    ..This review summarizes the present knowledge of bacterial tyrosinases, which are promising models in order to get more insights in structure, enzymatic reactions and functions of "type 3 copper" proteins in general...
  69. ncbi The tyrosinase gene and oculocutaneous albinism type 1 (OCA1): A model for understanding the molecular biology of melanin formation
    W S Oetting
    Department of Medicine, University of Minnesota, Minneapolis 55455, USA
    Pigment Cell Res 13:320-5. 2000
    ..Several questions still remain, including cryptic mutations that affect tyrosinase activity and the minimum amount of pigment required for normal optic development. The next 10 years should prove just as exciting as the last...
  70. ncbi Chemical analysis of melanins and its application to the study of the regulation of melanogenesis
    S Ito
    Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan
    Pigment Cell Res 13:103-9. 2000
    ..In conclusion, our HPLC microanalytical method for characterizing eumelanin and pheomelanin has become a useful tool for the study of melanogenesis...
  71. ncbi Zebrafish as a new model for phenotype-based screening of melanogenic regulatory compounds
    Tae Young Choi
    Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, South Korea
    Pigment Cell Res 20:120-7. 2007
    ..We suggest that the zebrafish system is a novel alternative to mammalian models, with several advantages including the rapidity, cost-effectiveness, and physiological relevance...
  72. ncbi Melanin synthesis in microorganisms--biotechnological and medical aspects
    Przemyslaw M Plonka
    Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Acta Biochim Pol 53:429-43. 2006
    ..This review summarizes current views on melanogenesis in Pro- and Eukaryotic microorganisms in terms of their biotechnological and biomedical importance...
  73. ncbi Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei
    Emilia Selinheimo
    VTT Technical Research Centre of Finland, Espoo, Finland
    FEBS J 273:4322-35. 2006
    ..T. reesei TYR2 was active on both l-tyrosine and l-dopa, and it showed broad substrate specificity...
  74. pmc Albinism in the domestic cat (Felis catus) is associated with a tyrosinase (TYR) mutation
    D L Imes
    Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis 95616, USA
    Anim Genet 37:175-8. 2006
    ..Taken together, our results suggest that the TYR gene corresponds to the colour locus in cats and its alleles, from dominant to recessive, are as follows: C (full colour) > c(b) (burmese) > or = c(s) (siamese) > c (albino)...
  75. ncbi Inhibition of melanogenesis versus antioxidant properties of essential oil extracted from leaves of Vitex negundo Linn and chemical composition analysis by GC-MS
    Huey Chun Huang
    Department of Medical Laboratory Science and Biotechnology, China Medical University, No 91 Hsueh Shih Road, Taichung City 40402, Taiwan
    Molecules 17:3902-16. 2012
    ..negundo Linn leaves decreased melanin production in B16F10 melanoma cells and showed potent antioxidant activities. The essential oil can thereby serve as an inhibitor of melanin synthesis and could also act as a natural antioxidant...
  76. ncbi Genomic structure and evolutionary conservation of the tyrosinase gene family from Fugu
    Agnès Camacho-Hübner
    ISREC Swiss Institute for Experimental Cancer Research, Chemin des Boveresses 155, 1066, Epalinges, Switzerland
    Gene 285:59-68. 2002
    ..Our results demonstrate the existence of all three tyrosinase family members in teleosts and underline the evolutionary conservation of the pigmentary system...
  77. pmc Inhibition of melanogenesis and antioxidant properties of Magnolia grandiflora L. flower extract
    Huey Chun Huang
    Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan
    BMC Complement Altern Med 12:72. 2012
    ..grandiflora L. flower extract on mushroom tyrosinase, B16F10 intracellular tyrosinase activity and melanin content were determined spectrophotometrically. Meanwhile, the antioxidative capacity of the flower extract was also investigated...
  78. ncbi New potent inhibitors of tyrosinase: novel clues to binding of 1,3,4-thiadiazole-2(3H)-thiones, 1,3,4-oxadiazole-2(3H)-thiones, 4-amino-1,2,4-triazole-5(4H)-thiones, and substituted hydrazides to the dicopper active site
    Usman Ghani
    Department of Medical Biochemistry, King Saud University, Riyadh 11461, Saudi Arabia
    Bioorg Med Chem 18:4042-8. 2010
    ..The current work may help develop new potent tyrosinase inhibitors against hyperpigmentation including potential insecticides...
  79. ncbi A reactive ortho-quinone generated by tyrosinase-catalyzed oxidation of the skin depigmenting agent monobenzone: self-coupling and thiol-conjugation reactions and possible implications for melanocyte toxicity
    Paola Manini
    Department of Organic Chemistry and Biochemistry, University of Naples Federico II, Via Cinthia 4, I 80126 Naples, Italy
    Chem Res Toxicol 22:1398-405. 2009
    ....
  80. ncbi Modulation of antioxidant defense by Alpinia galanga and Curcuma aromatica extracts correlates with their inhibition of UVA-induced melanogenesis
    Uraiwan Panich
    Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
    Cell Biol Toxicol 26:103-16. 2010
    ..Inhibiting cellular oxidative stress and improving antioxidant defenses might be the mechanisms by which the extracts yield the protective effects on UVA-dependent melanogenesis...
  81. ncbi Down-regulation of melanogenesis by phospholipase D2 through ubiquitin proteasome-mediated degradation of tyrosinase
    Akiko Kageyama
    Division of Dermatology, Clinical Molecular Medicine, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    J Biol Chem 279:27774-80. 2004
    ..This suggests that PLD2 may play an important role in regulating pigmentation in vivo...
  82. pmc Regulation of tyrosinase trafficking and processing by presenilins: partial loss of function by familial Alzheimer's disease mutation
    Runsheng Wang
    Huffington Center on Aging and Departments of Molecular and Human Genetics and Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 103:353-8. 2006
    ..Our results identify Tyr and related proteins as physiological substrates of PS and link gamma-secretase activity with intracellular protein transport...
  83. ncbi Tyrp1 and oculocutaneous albinism type 3
    R Sarangarajan
    Department of Dermatology, University of Cincinnati College of Medicine, Ohio 45267 0592, USA
    Pigment Cell Res 14:437-44. 2001
    ..Tyrp1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and melanocyte cell death. The current review is an attempt to consolidate our understanding of the role of Tyrp1 in the melanocyte...
  84. ncbi Glutaraldehyde activated eggshell membrane for immobilization of tyrosinase from Amorphophallus companulatus: application in construction of electrochemical biosensor for dopamine
    Sanket Tembe
    Department of Chemistry, University of Pune, Ganeshkhind, Pune 411007, India
    Anal Chim Acta 612:212-7. 2008
    ..19 V versus Ag/AgCl (3M KCl). Linearity was observed within the range of 50-250 microM with a detection limit of 25 microM...
  85. ncbi Cloning and functional analysis of ascidian Mitf in vivo: insights into the origin of vertebrate pigment cells
    Ichiro Yajima
    Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aramaki Aza Aoba, Aoba ku, Sendai, Miyagi 980 8578, Japan
    Mech Dev 120:1489-504. 2003
    ....
  86. ncbi Cloning and characterization of a tyrosinase gene from the white-rot fungus Pycnoporus sanguineus, and overproduction of the recombinant protein in Aspergillus niger
    Sonia Halaouli
    UMR 1163 INRA Université de Provence de Biotechnologie des Champignons Filamenteux, IFR 86 de Biotechnologie Agro Industrielle de Marseille, 163 Avenue de Luminy, Case 925, 13288 Marseille Cedex 09, France
    Appl Microbiol Biotechnol 70:580-9. 2006
    ..The best transformant obtained, A. niger D15#26-e, produced extracellular tyrosinase activities of 534 and 1,668 U l(-1) for monophenolase and diphenolase, respectively, which corresponded to a protein yield of ca. 20 mg l(-1)...
  87. pmc Functional dissection of the mouse tyrosinase locus control region identifies a new putative boundary activity
    Patricia Giraldo
    Department of Molecular and Cellular Biology, Centro Nacional de Biotecnologia CNB CSIC, Campus de Cantoblanco, 28049 Madrid, Spain
    Nucleic Acids Res 31:6290-305. 2003
    ....
  88. ncbi Development of electrochemical biosensor based on tyrosinase immobilized in composite biopolymeric film
    Sanket Tembe
    Department of Chemistry, University of Pune, Ganeshkhind, Pune 411 007, India
    Anal Biochem 349:72-7. 2006
    ..The analytical characteristics of this sensor, including linear range, lower detection limit, pH, and storage stability, are described. It has reusability up to 15 cycles and a shelf life of more than 2 months...
  89. ncbi Stem cell factor rescues tyrosinase expression and pigmentation in discreet anatomic locations in albino mice
    Jillian C Vanover
    Department of Pediatrics, University of Kentucky College of Medicine, The Markey Cancer Center, The Graduate Center for Toxicology, Lexington, KY, USA
    Pigment Cell Melanoma Res 22:827-38. 2009
    ..These findings suggest that c-kit signaling can overcome the c2j Tyr mutation in the ears and tails of aging animals and that UV resistance depends on accumulation of epidermal eumelanin...
  90. ncbi Additive effects of heat and p38 MAPK inhibitor treatment on melanin synthesis
    Dong Seok Kim
    Department of Medicine, College of Medicine, Chung Ang University, Seoul 156 756, Korea
    Arch Pharm Res 30:581-6. 2007
    ..We conclude that ERK activation and p38 MAPK inhibition can work in an additive manner to decrease melanogenesis...
  91. ncbi Costs of resistance: genetic correlations and potential trade-offs in an insect immune system
    S C Cotter
    Institute of Biological Sciences, University of Stirling, Stirling, UK
    J Evol Biol 17:421-9. 2004
    ..We speculate on a potential trade-off between defence against parasites and predators, mediated by larval colour, and its role in maintaining genetic variation in traits under natural selection...
  92. ncbi Oculocutaneous albinism type 1: the last 100 years
    William S Oetting
    Department of Medicine, University of Minnesota, Minneapolis, MN, USA
    Pigment Cell Res 16:307-11. 2003
    ..Though much of the research in albinism has involved OCA1, there are many unanswered questions about OCA1 and albinism, in general. The next 100 yr should still provide many surprises as did the first 100 yr...
  93. ncbi Inulavosin, a melanogenesis inhibitor, leads to mistargeting of tyrosinase to lysosomes and accelerates its degradation
    Hideaki Fujita
    Division of Pharmaceutical Cell Biology, Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi ku, Fukuoka, Japan
    J Invest Dermatol 129:1489-99. 2009
    ..Taken together, inulavosin inhibits melanogenesis as a result of mistargeting of tyrosinase to lysosomes...
  94. ncbi A dual mechanism of 4-hydroxy-5-methyl-3[2H]-furanone inhibiting cellular melanogenesis
    Hyo Jung Kim
    Department of Molecular Medicine, Kyungpook National University School of Medicine, Daegu, 700 422, Republic of Korea
    J Cosmet Sci 59:117-25. 2008
    ..Therefore, the antimelanogenic effects of HMF were best explained by a dual mechanism inhibiting tyrosinase gene expression and the enzyme activity of pre-existing tyrosinase...
  95. ncbi Interactions between environmental variables determine immunity in the Indian meal moth Plodia interpunctella
    Alison Triggs
    School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK
    J Anim Ecol 81:386-94. 2012
    ..7. Predictions of the effect of environmental or population change on immunity and disease dynamics based on laboratory experiments that only investigate the effects of single variable are likely to be inaccurate or even entirely wrong...
  96. ncbi Direct interaction of tyrosinase with Tyrp1 to form heterodimeric complexes in vivo
    Takeshi Kobayashi
    Department of Physiology, Nagoya University Graduate School of Medicine, Nagoya 466 8550, Japan
    J Cell Sci 120:4261-8. 2007
    ..Taken together, these data demonstrate that Tyrp1 interacts directly with Tyr in vivo, which may regulate the stability and trafficking of melanogenic enzymes and thus pigment synthesis...
  97. pmc Isolation and sequence of a cDNA clone for human tyrosinase that maps at the mouse c-albino locus
    B S Kwon
    Molecular Genetics Laboratory, Guthrie Research Institute, Sayre, PA 18840
    Proc Natl Acad Sci U S A 84:7473-7. 1987
    ..Southern blot analysis of DNA derived from newborn mice carrying lethal albino deletion mutations revealed that Pmel34 maps near or at the c-albino locus, the position of the structural gene for tyrosinase...
  98. ncbi Cross-linking and immobilisation of different proteins with recombinant Verrucomicrobium spinosum tyrosinase
    Michael Fairhead
    Empa, Swiss Federal Laboratories for Materials Testing and Research, Laboratory for Biomaterials, Lerchenfeldstrasse 5, St Gallen, CH 9014, Switzerland
    J Biotechnol 150:546-51. 2010
    ..The resulting aggregates can be collected by centrifugation and retain high levels of activity and may find applications in biocatalysis...
  99. pmc Antiviral, anti-parasitic, and cytotoxic effects of 5,6-dihydroxyindole (DHI), a reactive compound generated by phenoloxidase during insect immune response
    Picheng Zhao
    Department of Entomology and Plant Pathology, Oklahoma State University, 127 NRC, Stillwater, OK 74078, USA
    Insect Biochem Mol Biol 41:645-52. 2011
    ..Taken together, these data showed that proPO activation and DHI production is strongly toxic against various pathogens but can also damage host tissues and cells if not properly controlled...
  100. ncbi Autosomal albino chicken mutation (ca/ca) deletes hexanucleotide (-deltaGACTGG817) at a copper-binding site of the tyrosinase gene
    T Tobita-Teramoto
    Department of Biology, Keio University, Hiyoshi, Yokohama, Japan
    Poult Sci 79:46-50. 2000
    ..We speculate that the six-base deletion is responsible for the inactive tyrosinase in this line of albino chickens...
  101. ncbi The tyrosinase enhancer is activated by Sox10 and Mitf in mouse melanocytes
    Fabien Murisier
    Swiss Institute for Experimental Cancer Research ISREC, National Center of Competence in Research NCCR Molecular Oncology, Chemin des Boveresses 155, 1066 Epalinges, Switzerland
    Pigment Cell Res 20:173-84. 2007
    ..The results suggest that Sox10 directly, and Mitf, most likely indirectly, activate the tyrosinase enhancer, underlining the contribution of Sox10 to tyrosinase gene regulation in melanocytes...

Research Grants65

  1. MOLECULAR BASIS FOR SCHISTOSOME REPRODUCTIVE DEVELOPMENT
    Philip Loverde; Fiscal Year: 1991
    ..A well characterized eggshell gene will serve as a model. 4) Once the DNA elements that control gene expression are identified, attempts to identify the regulatory factors and the genes that encode them will be made...
  2. 2003 Conference Pan-American Society Pigment Cell Resea
    Ruth Halaban; Fiscal Year: 2003
    ..3. To provide partial support to students and junior faculty to attend the meeting. They request funds for the 2003 meeting as well as for the 2 subsequent PASPCR annual meetings in years 2004 and 2006. ..
  3. PROLIFERATION & MALIGNANT TRANSFORMATION OF MELANOCYTES
    Ruth Halaban; Fiscal Year: 2004
    ..Our functional studies may provide a basis for the design of new therapeutical agents for the treatment of patients with metastatic melanomas. ..
  4. Epigenetic Chromatin Changes as Melanoma Markers
    Ruth Halaban; Fiscal Year: 2006
    ..Altogether, the results are likely to generate a novel marker(s) that can be used to assess propensity for malignant transformation, tumor progression and/or sensitivity to chemotherapeutic drugs that target chromatin conformation. ..
  5. Multi-peptide Vaccine Administered with Cyclophosphamide for High-risk Melanoma
    CRAIG LEE SLINGLUFF; Fiscal Year: 2010
    ....
  6. Feline Model of an Inherited Craniofacial Abnormality
    Leslie Lyons; Fiscal Year: 2004
    ..Once identified, mutation analyses of these genes in the cat or other species will lead to understanding the genetics of facial development in humans. ..
  7. GENETIC RESOURCES FOR NON-HUMAN PRIMATES
    Leslie Lyons; Fiscal Year: 2007
    ..The rationale for this approach is that many primate researchers are unaware of the current primate resources and are unfamiliar with the use and advantages of genetics because it is outside their areas of expertise. ..
  8. VACCINES WITH MHC CLASS II-RESTRICTED MELANOMA PEPTIDES
    Craig Slingluff; Fiscal Year: 2004
    ....
  9. MULTI-EPITOPE MELANOMA VACCINES FOR CD4 AND CD8 T-CELLS
    Craig Slingluff; Fiscal Year: 2009
    ....
  10. MATERNAL MRNA LOCALIZATIONS IN A DETERMINATE EGG
    WILLIAM JEFFERY; Fiscal Year: 2002
    ..This investigation will provide new information on the regulation of cell division and cell fate determination during embryogenesis. ..
  11. MULTI-EPITOPE MELANOMA PEPTIDE VACCINATION WITH GM-CSF
    Craig Slingluff; Fiscal Year: 2004
    ..abstract_text> ..
  12. Role of Prostaglandins & Phospholipase A in melanocytes
    Glynis Scott; Fiscal Year: 2009
    ..We hypothesize that PG and phospholipases stimulate HM dendricity, MS transfer &pigmentation through different mechanisms that act synergistically to modulate cutaneous pigmentation following UVR. ..
  13. Targeted Molecular Therapeutics for Melanoma: CCI-779 and Bevacizumab
    Craig Slingluff; Fiscal Year: 2008
    ..The following proposal is a clinical trial to evaluate the effects of bevacizumab and CCI-779, which interfere with vascularization and tumor growth, for the treatment of melanoma. [unreadable] [unreadable] [unreadable]..
  14. Molecular Neurobiology of Drug Abuse
    DONALD KUHN; Fiscal Year: 2006
    ..Kuhn will remain extensively engaged in scientific mentoring activities. ..
  15. Trace Element Metabolism: Basic and Applied Research
    MICHAEL PETRIS; Fiscal Year: 2006
    ..These individuals will be encouraged to present posters to be displayed in 2 poster sessions. We also will obtain funds to provide travel awards for up to 10 postdocs or senior graduate students. [unreadable] [unreadable] [unreadable]..
  16. AFP-Based Immunotherapy for Hepatocellular Carcinoma
    Antoni Ribas; Fiscal Year: 2006
    ..In summary, I propose a clinically-oriented research program that translates this original work into novel, evidence-based immnunotherapy trials for hepatocellular carcinoma. ..
  17. Complement inhibition in experimental autoimmune uveitis
    RUSSELL READ; Fiscal Year: 2006
    ..abstract_text> ..
  18. Antigenic determinants on HLA-B7 and-A2
    VICTOR ENGELHARD; Fiscal Year: 2006
    ..Collectively, these studies will continue to use mass spectrometry to offer insight into the display of peptides by MHC molecules of cancer cells, and the relevance of this display to control of tumors by the immune system. ..
  19. Bromelain Proteinase Activity in IBD
    LAURA HALE; Fiscal Year: 2006
    ..They will thus strengthen the scientific knowledge base needed to inform the practice of complementary and alternative medicine. ..
  20. Regulation of Mammalian Copper Homeotasis
    MICHAEL PETRIS; Fiscal Year: 2007
    ..The implication of copper in Alzheimer's disease, prion diseases, and several genetic disorders, suggests the study of hCtr1 may have far-reaching implications for the improvement of human health. ..
  21. Two-Photon Microscopy for Biomedical Research
    BENJAMIN REESE; Fiscal Year: 2007
    ..Acquisition of this 2-photon microscope will therefore enhance the diverse efforts of a number of investigators at UCSB in pursuit of their NIH-funded research objectives. [unreadable] [unreadable] [unreadable]..
  22. A tyrosinase-targeted drug design for the treatment of melanoma
    Majid Moridani; Fiscal Year: 2007
    ..In this work we will be developing novel phenol based drugs for treatment of melanoma. [unreadable] [unreadable] [unreadable]..
  23. Dispersion Patterns for Retinal Neuroblasts
    BENJAMIN REESE; Fiscal Year: 2009
    ..abstract_text> ..
  24. Function of Laccases in Anopheles gambiae
    Michael R Kanost; Fiscal Year: 2010
    ..Disrupting laccase function may provide a new mechanism for controlling mosquito populations. ..
  25. Chemoprevention of IBD-Associated Colon Cancer
    Laura P Hale; Fiscal Year: 2010
    ..The proposed studies will further elucidate mechanisms that can lead to novel interventions to prevent IBD-associated colon cancers, providing great benefit to IBD patients and their families. ..
  26. Altered CD8+ T cell Compartment in SLE Patients
    JACQUES F BANCHEREAU; Fiscal Year: 2010
    ..These studies will help us conclude whether killer CD8+T cells and/or suppressor CD8+ T cells i) can be used as markers and/or predictors of disease activity, ii) represent a valuable new option in lupus therapy. ..
  27. PROTEINASE SYSTEMS IN INSECT HEMOLYMPH
    Michael R Kanost; Fiscal Year: 2010
    ..sexta, to apply this knowledge to insect vectors of human diseases, and to apply advantages of the M. sexta system for fundamental studies on the regulation of serine protease activity. ..
  28. Regulation of Mammalian Copper Homeostasis
    MICHAEL PETRIS; Fiscal Year: 2008
    ..This research will shed light on the importance of copper in the pathogenesis of Alzheimer's disease, and whether the availability of this nutrient should be investigated in therapeutic strategies. ..
  29. Mast Cells and IBD Pathogenesis
    LAURA HALE; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  30. Genetic networks that regulate the mast lineage
    Clifford Takemoto; Fiscal Year: 2009
    ..This proposal will provide insights into the mechanisms of lineage-selection during mast cell development and may lead to therapeutic targets for mast cell-related diseases. ..
  31. B7-DC Cross-linking antibody immunotherapy
    Svetomir Markovic; Fiscal Year: 2008
    ..If successful, this "proof-of-principle" clinical trial will justify further development of B7-DC XAb as a cancer therapeutic with potential universal application for all malignant disorders. [unreadable] [unreadable] [unreadable]..
  32. SUBPOPULATIONS OF HUMAN DENDRITIC CELLS
    Jacques Banchereau; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  33. 19th International Pigment Cell Conference - 2005
    Zalfa Abdel Malek; Fiscal Year: 2005
    ..abstract_text> ..
  34. Novel Oral Adjuvant for Dental Vaccines
    LAURA HALE; Fiscal Year: 2005
    ..abstract_text> ..
  35. Bromelain as a Biologically-Based Therapy for IBD
    LAURA HALE; Fiscal Year: 2005
    ..This research will thus strengthen the biomedical research knowledge base needed to inform the practice of complementary and alternative medicine. ..
  36. 2004 National Vitiligo Foundation Scientific Conference
    Raymond Boissy; Fiscal Year: 2004
    ..In addition, mini-symposiums will be organized for each of the sessions. ..
  37. Fine Needle Aspiration Biopsy(FNAB) Needle Device
    Vinod Shidham; Fiscal Year: 2002
    ..Apart from the worldwide market, the potential market in United States alone should exceed $1,500,000 per year. ..
  38. Dendritic Cells, Heat Shock Proteins and Tumor Immunity
    Jacques Banchereau; Fiscal Year: 2004
    ..These studies will permit us to produce an altered melanoma cell line, with enhanced immunogenicity, that will be used to generate tumor cell bodies to load onto DCs for vaccination protocols. ..
  39. IDENTIFICATION OF HUMAN MINOR H ANTIGEN PEPTIDES & GENES
    VICTOR ENGELHARD; Fiscal Year: 2003
    ..abstract_text> ..
  40. REACTIVE OXYGEN SPECIES AND DROSOPHILA IMMUNITY
    A Nappi; Fiscal Year: 2001
    ..abstract_text> ..
  41. MOLECULAR ORIGIN OF CANCER--CATECHOL/SEMIQUINOE/QUINONE
    Ercole Cavalieri; Fiscal Year: 2003
    ..In addition, we will identify biomarkers for use in diagnosis and in studies of prevention of breast cancer and other diseases. ..
  42. CATECHOLAMINES AND DEVELOPMENT
    Suzanne Roffler Tarlov; Fiscal Year: 2002
    ..They will test the hypothesis that norepinephrine induces the differentiation factor that directs the switch from an adrenergic to a cholinergic phenotype in sympathetically-innervated sweat glands. ..
  43. PHENOLS/CATECHOLS IN OCCUPATIONAL/CONTACT VITILIGO SKIN
    Raymond Boissy; Fiscal Year: 2002
    ....
  44. MICROARRAY ANALYSIS OF HUMAN COCAINE ADDICTS
    DONALD KUHN; Fiscal Year: 2002
    ..Microarray-based scanning of the human genome has the potential to establish those genes or gene clusters that define the addictive phenotype, leading the way to proteomic-based studies of the bases of addiction. ..
  45. Dendritic Cell Vaccine in Metastatic Prostate Cancer
    Jacques Banchereau; Fiscal Year: 2002
    ..This study will permit us to establish the safety and tolerability of DCs loaded with killed allogeneic tumor cells as a source of TAA. It will also permit us to gain insights into the level of prostate cancer immunity induced by DCs. ..
  46. Molecular Orgin Cancer--Catechol Estrogen 3 4 Quinones
    Ercole Cavalieri; Fiscal Year: 2004
    ..The knowledge acquired from these studies will be essential for developing preventive strategies. ..
  47. Pilot Studies in Biochemistry of Anopheles gambiae
    Michael Kanost; Fiscal Year: 2005
    ..gambiae serpins and laccases. This project will be a start toward development of a new research area for the PI (mosquito biochemistry) that will build from his experience in biochemistry of other groups of insects. ..
  48. Virulence Factor Trafficking in Cryptococcus
    Peter Williamson; Fiscal Year: 2005
    ..Each of the mutants identified in Specific Aims 2 and 3 will then be assessed for production of other virulence-related factors such as capsule, urease, phospholipase and virulence in a mouse model. ..
  49. Spatial Relationships Between Neurons in the CNS
    BENJAMIN REESE; Fiscal Year: 2005
    ..abstract_text> ..
  50. CONSTRUCTION AND APPLICATION OF A US ADMIXTURE MAP
    Mark Shriver; Fiscal Year: 2006
    ..From simulation studies, we estimate that about 1000 AIMs will be required for genome-wide mapping with two-way admixture, and about 1500 for admixture between three populations. [unreadable] [unreadable] [unreadable]..
  51. ROLE OF THE PTEN PATHWAY IN MELANOMA
    Marcus Bosenberg; Fiscal Year: 2005
    ..He has also completed Ph.D. training in cell biology. The proposed research will be carried out in the laboratory of Dr. Ronald DePinho at the Dana-Farber Cancer Institute, an affiliate of the Harvard Medical School. ..
  52. Mice with Human Dendritic Cells to Test Vaccine Potency
    A Palucka; Fiscal Year: 2004
    ..The experiments proposed here would lay ground for further use of SCID-hu/DCs mice to study other vaccines and demonstrate development of protective immunity upon in vivo rechallenge. ..
  53. Dendritic Cells and Breast Cancer Immunity
    A Palucka; Fiscal Year: 2004
    ..Overall, this proposal will permit us to demonstrate that efficient breast cancer immunity can be generated and used to reject the tumor thus permitting development of novel treatment modalities in breast cancer. ..
  54. NEUROSCIENCE TRAINING IN DRUG ABUSE
    DONALD KUHN; Fiscal Year: 2004
    ..abstract_text> ..
  55. Albinism: Defects in Tyrosinase, Amines, or Melanin
    Suzanne Roffler Tarlov; Fiscal Year: 2005
    ..Ultimately, if such signals deriving from tyrosinase-driven pathway unrelated to melanin production, are found, this research will open doors to therapeutic intervention in individuals carrying the OA1 gene. ..
  56. Gene Polymorphisms and Kidney Transplant Outcome
    William Oetting; Fiscal Year: 2005
    ..abstract_text> ..
  57. HPV DNA VACCINE FOR CERVICAL PRE NEOPLASIA
    Jeffrey Weber; Fiscal Year: 2003
    ..To insure that spontaneous regression does not account for a portion of the responses, baseline measurements will be followed one month later by a second set of assessments prior to the start of the vaccine. ..
  58. Dendritic Cell Vaccination During Lymphoid Reconstitution
    Jeffrey Weber; Fiscal Year: 2008
    ..Toxicity as well as clinical response will be secondary endpoints. The cohort with the optimal level of immune response will be taken forward in future studies. ..
  59. Genetic Modulation of Noise Injury to the Cochlear Lateral Wall
    KEVIN OHLEMILLER; Fiscal Year: 2009
    ..The gene, and the processes in which it is involved, may affect the long-term stability of cochlear injury, and the accumulation of injury that may be diagnosed as presbycusis. ..
  60. CTLA-4 Inhibition and Autoimmunity in Melanoma
    Jeffrey Weber; Fiscal Year: 2008
    ..abstract_text> ..
  61. IL-12 as an Adjuvant for a Melanoma Peptide Vaccine
    Jeffrey Weber; Fiscal Year: 2003
    ..The results of those assays will be used to design randomized phase III trials for resected high risk melanoma and to develop strategies for overcoming antigen-specific unresponsiveness in melanoma patients. ..
  62. Genetic Modulation of Noise Injury to the Cochlear Lateral Wall
    KEVIN OHLEMILLER; Fiscal Year: 2007
    ..The gene, and the processes in which it is involved, may affect the long-term stability of cochlear injury, and the accumulation of injury that may be diagnosed as presbycusis. ..
  63. COCHLEAR VULNERABILITY/REACTIVE OXYGEN SPECIES
    KEVIN OHLEMILLER; Fiscal Year: 2003
    ..Our experiments will establish how well the dynamics of ROS production predict cochlear injury, and whether progressive deafness genes may impair cochlear ROS regulation. ..
  64. IN VITRO/IN VIVO ACTIVATED DENDRITIC CELLS FOR MELANOMA
    Jeffrey Weber; Fiscal Year: 2003
    ....
  65. Role of CRH in skin physiology and pathology
    Andrzej Slominski; Fiscal Year: 2004
    ..g., melanocytes and keratinocytes, and test the role of CRH/CRH-R1 axis in the regulation of human hair growth. ..