hydrogenase

Summary

Summary: An enzyme found in bacteria. It catalyzes the reduction of ferredoxin and other substances in the presence of molecular hydrogen and is involved in the electron transport of bacterial photosynthesis. EC 1.18.99.1.

Top Publications

  1. ncbi Occurrence, classification, and biological function of hydrogenases: an overview
    Paulette M Vignais
    CEA Grenoble, Laboratoire de Biochimie et Biophysique des Systemes Integres, UMR CEA CNRS UJF 5092, Institut de Recherches en Technologies et Sciences pour le Vivant iRTSV, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
    Chem Rev 107:4206-72. 2007
  2. pmc The iron-hydrogenase of Thermotoga maritima utilizes ferredoxin and NADH synergistically: a new perspective on anaerobic hydrogen production
    Gerrit J Schut
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602 7229, USA
    J Bacteriol 191:4451-7. 2009
  3. ncbi [FeFe]-hydrogenase in Yellowstone National Park: evidence for dispersal limitation and phylogenetic niche conservatism
    Eric S Boyd
    Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, 59717, USA
    ISME J 4:1485-95. 2010
  4. doi The crystal structure of an oxygen-tolerant hydrogenase uncovers a novel iron-sulphur centre
    Johannes Fritsch
    Mikrobiologie, Institut fur Biologie, Humboldt Universitat zu Berlin, Chausseestraße 117, 10115 Berlin, Germany
    Nature 479:249-52. 2011
  5. pmc Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii
    A Melis
    Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, Berkeley, California 94720 3102, USA
    Plant Physiol 122:127-36. 2000
  6. pmc Rewiring hydrogenase-dependent redox circuits in cyanobacteria
    Daniel C Ducat
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 108:3941-6. 2011
  7. doi Structural basis for a [4Fe-3S] cluster in the oxygen-tolerant membrane-bound [NiFe]-hydrogenase
    Yasuhito Shomura
    Department of Life Science, Graduate School of Life Science, University of Hyogo, 3 2 1 Koto, Kamigori cho, Ako gun, Hyogo 678 1297, Japan
    Nature 479:253-6. 2011
  8. doi RNA silencing of hydrogenase(-like) genes and investigation of their physiological roles in the green alga Chlamydomonas reinhardtii
    James E Godman
    Department of Plant Sciences, University of Cambridge, UK
    Biochem J 431:345-51. 2010
  9. doi Production of biohydrogen by heterologous expression of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli
    Jaoon Y H Kim
    Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790 784, Republic of Korea
    J Biotechnol 155:312-9. 2011
  10. pmc Identification of the [FeFe]-hydrogenase responsible for hydrogen generation in Thermoanaerobacterium saccharolyticum and demonstration of increased ethanol yield via hydrogenase knockout
    A Joe Shaw
    Dartmouth College, Thayer School of Engineering, 8000 Cummings Hall, Hanover, NH 03755, USA
    J Bacteriol 191:6457-64. 2009

Research Grants

Detail Information

Publications311 found, 100 shown here

  1. ncbi Occurrence, classification, and biological function of hydrogenases: an overview
    Paulette M Vignais
    CEA Grenoble, Laboratoire de Biochimie et Biophysique des Systemes Integres, UMR CEA CNRS UJF 5092, Institut de Recherches en Technologies et Sciences pour le Vivant iRTSV, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
    Chem Rev 107:4206-72. 2007
  2. pmc The iron-hydrogenase of Thermotoga maritima utilizes ferredoxin and NADH synergistically: a new perspective on anaerobic hydrogen production
    Gerrit J Schut
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602 7229, USA
    J Bacteriol 191:4451-7. 2009
    ..The trimeric [FeFe]-type hydrogenase that was previously purified from T...
  3. ncbi [FeFe]-hydrogenase in Yellowstone National Park: evidence for dispersal limitation and phylogenetic niche conservatism
    Eric S Boyd
    Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, 59717, USA
    ISME J 4:1485-95. 2010
    ..In this study, we examined the [FeFe]-hydrogenase gene (hydA) as a proxy for fermentative bacterial H₂ production along physical and chemical gradients in ..
  4. doi The crystal structure of an oxygen-tolerant hydrogenase uncovers a novel iron-sulphur centre
    Johannes Fritsch
    Mikrobiologie, Institut fur Biologie, Humboldt Universitat zu Berlin, Chausseestraße 117, 10115 Berlin, Germany
    Nature 479:249-52. 2011
    ..Here we present the crystal structure of an O(2)-tolerant [NiFe]-hydrogenase from the aerobic H(2) oxidizer Ralstonia eutropha H16 at 1.5 Å resolution...
  5. pmc Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii
    A Melis
    Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, Berkeley, California 94720 3102, USA
    Plant Physiol 122:127-36. 2000
    The work describes a novel approach for sustained photobiological production of H(2) gas via the reversible hydrogenase pathway in the green alga Chlamydomonas reinhardtii...
  6. pmc Rewiring hydrogenase-dependent redox circuits in cyanobacteria
    Daniel C Ducat
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 108:3941-6. 2011
    ..increasing the photobiological production of hydrogen within cyanobacterial cultures, we expressed the [FeFe] hydrogenase, HydA, from Clostridium acetobutylicum in the non-nitrogen-fixing cyanobacterium Synechococcus elongatus sp...
  7. doi Structural basis for a [4Fe-3S] cluster in the oxygen-tolerant membrane-bound [NiFe]-hydrogenase
    Yasuhito Shomura
    Department of Life Science, Graduate School of Life Science, University of Hyogo, 3 2 1 Koto, Kamigori cho, Ako gun, Hyogo 678 1297, Japan
    Nature 479:253-6. 2011
    Membrane-bound respiratory [NiFe]-hydrogenase (MBH), a H(2)-uptake enzyme found in the periplasmic space of bacteria, catalyses the oxidation of dihydrogen: H(2) → 2H(+) + 2e(-) (ref. 1)...
  8. doi RNA silencing of hydrogenase(-like) genes and investigation of their physiological roles in the green alga Chlamydomonas reinhardtii
    James E Godman
    Department of Plant Sciences, University of Cambridge, UK
    Biochem J 431:345-51. 2010
    The genome of the green alga Chlamydomonas reinhardtii encodes two [FeFe]-hydrogenases, HydA1 and HydA2, and the hydrogenase-like protein HYD3...
  9. doi Production of biohydrogen by heterologous expression of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli
    Jaoon Y H Kim
    Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790 784, Republic of Korea
    J Biotechnol 155:312-9. 2011
    Oxygen sensitivity of hydrogenase is a critical issue in efficient biological hydrogen production...
  10. pmc Identification of the [FeFe]-hydrogenase responsible for hydrogen generation in Thermoanaerobacterium saccharolyticum and demonstration of increased ethanol yield via hydrogenase knockout
    A Joe Shaw
    Dartmouth College, Thayer School of Engineering, 8000 Cummings Hall, Hanover, NH 03755, USA
    J Bacteriol 191:6457-64. 2009
    Three putative hydrogenase enzyme systems in Thermoanaerobacterium saccharolyticum were investigated at the genetic, mRNA, enzymatic, and phenotypic levels...
  11. pmc Heterologous expression and maturation of an NADP-dependent [NiFe]-hydrogenase: a key enzyme in biofuel production
    Junsong Sun
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 5:e10526. 2010
    ..Microbial hydrogen production is catalyzed by hydrogenase, an extremely complex, air-sensitive enzyme that utilizes a binuclear nickel-iron [NiFe] catalytic site...
  12. ncbi Molecular hydrogen as an energy source for Helicobacter pylori
    Jonathan W Olson
    Department of Microbiology, University of Georgia, Athens, GA 30602, USA
    Science 298:1788-90. 2002
    ..pylori. Hydrogenase activity in H. pylori is constitutive but increased fivefold upon incubation with hydrogen...
  13. pmc Flexibility in anaerobic metabolism as revealed in a mutant of Chlamydomonas reinhardtii lacking hydrogenase activity
    Alexandra Dubini
    Environmental Science and Engineering Division, Colorado School of Mines, Golden, Colorado 80401, USA
    J Biol Chem 284:7201-13. 2009
    ..b>Hydrogenase activity is an important component of this metabolism, and we have compared metabolic and regulatory responses ..
  14. doi Efficient electron transfer from hydrogen to benzyl viologen by the [NiFe]-hydrogenases of Escherichia coli is dependent on the coexpression of the iron-sulfur cluster-containing small subunit
    Constanze Pinske
    Institute for Microbiology, Martin Luther University Halle Wittenberg, Kurt Mothes Str 3, 06120, Halle, Saale, Germany
    Arch Microbiol 193:893-903. 2011
    ..coli [NiFe]-hydrogenases restored activity of the respective hydrogenase to strain FTD147, which carries in-frame deletions in the hyaB, hybC, and hycE genes encoding the large ..
  15. ncbi HypF, a carbamoyl phosphate-converting enzyme involved in [NiFe] hydrogenase maturation
    Athanasios Paschos
    Mikrobiologie, Department I der Fakultät für Biologie, Ludwig Maximilians Universitat Munchen, Maria Ward Strasse 1a, D 80638 Munich, Germany
    J Biol Chem 277:49945-51. 2002
    ..the derived amino acid sequence showed a full correlation of the resulting variants between their activity in hydrogenase maturation and the in vitro reactivity with carbamoyl phosphate...
  16. pmc Distinct physiological roles of the three [NiFe]-hydrogenase orthologs in the hyperthermophilic archaeon Thermococcus kodakarensis
    Tamotsu Kanai
    Department of Biotechnology, College of Life Sciences, Ritsumeikan University, Kusatsu 525 8577, Japan
    J Bacteriol 193:3109-16. 2011
    ..with the reduction of elemental sulfur (S⁰) or H₂ generation, harbors three gene operons encoding [NiFe]-hydrogenase orthologs, namely, Hyh, Mbh, and Mbx...
  17. ncbi Insights into [FeFe]-hydrogenase structure, mechanism, and maturation
    David W Mulder
    Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, MT 59717, USA
    Structure 19:1038-52. 2011
    ..The wealth of structural information on different classes and different states of hydrogenase enzymes, biosynthetic intermediates, and maturation enzymes has contributed significantly to understanding the ..
  18. ncbi Engineering a non-native hydrogen production pathway into Escherichia coli via a cyanobacterial [NiFe] hydrogenase
    Mark A Wells
    ChELSI Institute, Department of Chemical and Biological Engineering, University of Sheffield, Mappin Street, UK
    Metab Eng 13:445-53. 2011
    ..Hydrogen production is observed in vivo in a hydrogenase null host, demonstrating coupling to host electron transfer systems...
  19. ncbi The hows and whys of aerobic H2 metabolism
    Alison Parkin
    Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, Oxford OX1 3QR, UK
    Curr Opin Chem Biol 16:26-34. 2012
    ..In light of recent structural data and mutagenic studies this article reviews the molecular mechanism of O2-tolerance in [NiFe]-hydrogenases and discusses the biosynthesis of the unique Fe-S relay...
  20. ncbi The hyperthermophilic bacterium, Thermotoga maritima, contains an unusually complex iron-hydrogenase: amino acid sequence analyses versus biochemical characterization
    M F Verhagen
    Department of Biochemistry and Molecular Biology and the Center for Metalloenzyme Studies, Life Sciences Building, The University of Georgia, Athens, GA, USA
    Biochim Biophys Acta 1412:212-29. 1999
    ..The H(2)-evolving cytoplasmic hydrogenase of this organism was shown to consist of three different subunits of masses 73 (alpha), 68 (beta) and 19 (gamma)..
  21. ncbi Hydrogen fuel production by transgenic microalgae
    Anastasios Melis
    University of California, Department of Plant and Microbial Biology, 111 Koshland Hall, Berkeley, California 94720 3102, USA
    Adv Exp Med Biol 616:110-21. 2007
    ..Application of the green algal hydrogenase assembly genes is examined in efforts to confer H2-production capacity to other commercially significant ..
  22. ncbi The biosynthetic routes for carbon monoxide and cyanide in the Ni-Fe active site of hydrogenases are different
    Winfried Roseboom
    Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, NL 1018 WV Amsterdam, The Netherlands
    FEBS Lett 579:469-72. 2005
    ..The results suggest that the biosynthetic routes of the carbon monoxide and cyanide ligands in [NiFe]-hydrogenases are different...
  23. ncbi Structure/function relationships of [NiFe]- and [FeFe]-hydrogenases
    Juan C Fontecilla-Camps
    Laboratoire de Cristallographie et Cristallogenese des Proteines, Institut de Biologie Structurale J P Ebel, CEA, CNRS, Universite Joseph Fourier, 41 rue J Horowitz, 38027 Grenoble Cedex 1, France
    Chem Rev 107:4273-303. 2007
  24. ncbi Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster
    J C Boyington
    Laboratory of Molecular Structure, National Institute of Allergy and Infectious Diseases, National Institutes of Health NIH, Rockville, MD 20852, USA
    Science 275:1305-8. 1997
    ..These structures suggest a reaction mechanism that directly involves SeCys140 and His141 in proton abstraction and the molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron transfer...
  25. doi Dithiomethylether as a ligand in the hydrogenase h-cluster
    Arti S Pandey
    Department of Chemistry and Biochemistry and Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, Montana 59717, USA
    J Am Chem Soc 130:4533-40. 2008
    An X-ray crystallographic refinement of the H-cluster of [FeFe]-hydrogenase from Clostridium pasteurianum has been carried out to close-to atomic resolution and is the highest resolution [FeFe]-hydrogenase presented to date. The 1...
  26. ncbi Carbamoylphosphate serves as the source of CN(-), but not of the intrinsic CO in the active site of the regulatory [NiFe]-hydrogenase from Ralstonia eutropha
    Oliver Lenz
    Humboldt Universitat zu Berlin, Institut für Biologie Mikrobiologie, Chausseestrasse 117, Berlin, Germany
    FEBS Lett 581:3322-6. 2007
    ..To identify the metabolic origins of these ligands, the regulatory [NiFe] hydrogenase in conjunction with the indigenous Hyp maturation proteins of Ralstonia eutropha H16 were heterologously ..
  27. pmc Hydrogen production in Chlamydomonas: photosystem II-dependent and -independent pathways differ in their requirement for starch metabolism
    Vincent Chochois
    Commissariat a l Energie Atomique, Direction des Sciences du Vivant, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Commissariat à l Energie Atomique Cadarache, F 13108 Saint Paul lez Durance, France
    Plant Physiol 151:631-40. 2009
    ..In the direct pathway, photosystem II (PSII) supplies electrons to hydrogenase through the photosynthetic electron transport chain, while in the indirect pathway, hydrogen is produced in the ..
  28. ncbi Investigating and exploiting the electrocatalytic properties of hydrogenases
    Kylie A Vincent
    Inorganic Chemistry Laboratory, Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QR, United Kingdom
    Chem Rev 107:4366-413. 2007
  29. ncbi Maturation of [NiFe]-hydrogenases in Escherichia coli
    Lucia Forzi
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
    Biometals 20:565-78. 2007
    ..in synthesis and attachment of these ligands to the iron atom, insertion of the Fe(CN)2CO group into the apo-hydrogenase, introduction of the nickel atom into the pre-formed active site and ensuring that the holoenzyme is correctly ..
  30. ncbi Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms
    Maria L Ghirardi
    National Renewable Energy Laboratory, Golden, CO 80401, USA
    Annu Rev Plant Biol 58:71-91. 2007
    ..In these organisms, photosynthetic water splitting is functionally linked to H(2) production by the activity of hydrogenase enzymes...
  31. ncbi Requirement of nickel metabolism proteins HypA and HypB for full activity of both hydrogenase and urease in Helicobacter pylori
    J W Olson
    Department of Microbiology, 813 Biological Sciences Building, University of Georgia, Athens, GA 30602, USA
    Mol Microbiol 39:176-82. 2001
    The nickel-containing enzymes hydrogenase and urease require accessory proteins in order to incorporate properly the nickel atom(s) into the active sites...
  32. ncbi Construction and characterization of ack deleted mutant of Clostridium tyrobutyricum for enhanced butyric acid and hydrogen production
    Xiaoguang Liu
    Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Prog 22:1265-75. 2006
    ..that the AK activity in PAK-Em decreased by approximately 50%; meanwhile, phosphotransacetylase (PTA) and hydrogenase activities each increased by approximately 40%...
  33. pmc Hitherto unknown [Fe-Fe]-hydrogenase gene diversity in anaerobes and anoxic enrichments from a moderately acidic fen
    Oliver Schmidt
    Department of Ecological Microbiology, University of Bayreuth, 95445 Bayreuth, Germany
    Appl Environ Microbiol 76:2027-31. 2010
    ..Comparative analysis of [Fe-Fe]-hydrogenase and 16S rRNA gene-based phylogenies indicated the presence of homologous multiple hydrogenases per organism and ..
  34. pmc X-ray crystallographic and computational studies of the O2-tolerant [NiFe]-hydrogenase 1 from Escherichia coli
    Anne Volbeda
    Metalloproteins Unit, Institut de Biologie Structurale J P Ebel, Commissariat à l Energie Atomique Centre National de la Recherche Scientifique l Université Joseph Fourier, 41 rue Jules Horowitz, 38027 Grenoble, France
    Proc Natl Acad Sci U S A 109:5305-10. 2012
    The crystal structure of the membrane-bound O(2)-tolerant [NiFe]-hydrogenase 1 from Escherichia coli (EcHyd-1) has been solved in three different states: as-isolated, H(2)-reduced, and chemically oxidized...
  35. doi The Escherichia coli metal-binding chaperone SlyD interacts with the large subunit of [NiFe]-hydrogenase 3
    Kim C Chan Chung
    Department of Chemistry, University of Toronto, Toronto, ON, Canada
    FEBS Lett 585:291-4. 2011
    The multi-step biosynthesis of the [NiFe]-hydrogenase enzyme involves a variety of accessory proteins...
  36. doi Alcohol dehydrogenase and hydrogenase transcript fluctuations during a day-night cycle in Chlamydomonas reinhardtii: the role of anoxia
    Larisa Angela Swirsky Whitney
    Department of Crop Plant Biology, University of Pisa, Pisa, Italy
    New Phytol 190:488-98. 2011
    ..The cell cycle and light-dark cycles are equally important elements in the regulatory network modulating the anaerobic response in Chlamydomonas...
  37. ncbi Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath)
    R Csáki
    Department of Biotechnology, University of Szeged, Hungary
    FEMS Microbiol Lett 205:203-7. 2001
    The first gene cluster encoding for a membrane bound [NiFe] hydrogenase from a methanotroph, Methylococcus capsulatus (Bath), was cloned and sequenced...
  38. doi A unique iron-sulfur cluster is crucial for oxygen tolerance of a [NiFe]-hydrogenase
    Tobias Goris
    Institut für Biologie Mikrobiologie, Humboldt Universitat zu Berlin, Berlin, Germany
    Nat Chem Biol 7:310-8. 2011
    ..The O(2)-tolerant membrane-bound [NiFe]-hydrogenase of Ralstonia eutropha H16 is one of the few examples that can perform H(2) uptake in the presence of ambient O(..
  39. ncbi Sodium ion-dependent hydrogen production in Acidaminococcus fermentans
    U Härtel
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, D 35032 Marburg, Germany
    Arch Microbiol 166:350-6. 1996
    ..The acetate/butyrate ratio thereby increased from 2.0 at 1 mM Na+ to 3.0 at 100 mM Na+. A hydrogenase and an NADH dehydrogenase, both of which were detected in membrane fractions, are components of a model in ..
  40. pmc Mechanism of proton transfer in [FeFe]-hydrogenase from Clostridium pasteurianum
    Adam J Cornish
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824 1319, USA
    J Biol Chem 286:38341-7. 2011
    ..Previously, structural characterization of [FeFe]-hydrogenase from Clostridium pasteurianum indicated a potential proton transport pathway involving four residues (Cys-299, ..
  41. ncbi A model system for [NiFe] hydrogenase maturation studies: Purification of an active site-containing hydrogenase large subunit without small subunit
    Gordon Winter
    Institut für Biologie Mikrobiologie, Humboldt Universitat zu Berlin, Chausseestr 117, D 10115 Berlin, Germany
    FEBS Lett 579:4292-6. 2005
    The large subunit HoxC of the H2-sensing [NiFe] hydrogenase from Ralstonia eutropha was purified without its small subunit. Two forms of HoxC were identified. Both forms contained iron but only substoichiometric amounts of nickel...
  42. pmc Photosynthetic electron partitioning between [FeFe]-hydrogenase and ferredoxin:NADP+-oxidoreductase (FNR) enzymes in vitro
    Iftach Yacoby
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Proc Natl Acad Sci U S A 108:9396-401. 2011
    Photosynthetic water splitting, coupled to hydrogenase-catalyzed hydrogen production, is considered a promising clean, renewable source of energy...
  43. doi Growth- and substrate-dependent transcription of formate dehydrogenase and hydrogenase coding genes in Syntrophobacter fumaroxidans and Methanospirillum hungatei
    Petra Worm
    Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands
    Microbiology 157:280-9. 2011
    ..Our results strongly suggest that in syntrophically grown S. fumaroxidans cells, the [FeFe]-hydrogenase (encoded by Sfum_844-46), FDH1 (Sfum_2703-06) and Hox (Sfum_2713-16) may confurcate electrons from NADH and ..
  44. pmc Control of hydrogen photoproduction by the proton gradient generated by cyclic electron flow in Chlamydomonas reinhardtii
    Dimitri Tolleter
    Commissariat à l Énergie Atomique et aux Énergies Alternatives, Direction des Sciences du Vivant, Institut de Biologie Environementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Commissariat à l Energie Atomique Cadarache, 13108 Saint Paul Lez Durance, France
    Plant Cell 23:2619-30. 2011
    ..microalgae results from a connection between the photosynthetic electron transport chain and a plastidial hydrogenase. Algal H₂ production is a transitory phenomenon under most natural conditions, often viewed as a safety valve ..
  45. doi Heterologous expression of Alteromonas macleodii and Thiocapsa roseopersicina [NiFe] hydrogenases in Escherichia coli
    P D Weyman
    Department of Synthetic Biology and Bioenergy, The J Craig Venter Institute, Rockville, MD 20850, USA
    Microbiology 157:1363-74. 2011
    HynSL from Alteromonas macleodii 'deep ecotype' (AltDE) is an oxygen-tolerant and thermostable [NiFe] hydrogenase. Its two structural genes (hynSL), encoding small and large hydrogenase subunits, are surrounded by eight genes (hynD, hupH ..
  46. ncbi The CO and CN(-) ligands to the active site Fe in [NiFe]-hydrogenase of Escherichia coli have different metabolic origins
    Lucia Forzi
    Max Planck Institut fur terrestrische Mikrobiologie, Karl von Frisch Str, Marburg, Germany
    FEBS Lett 581:3317-21. 2007
    ..To determine their metabolic origin, [NiFe]-hydrogenase-2 was isolated from Escherichia coli grown in the presence of L-[ureido-(13)C]citrulline, purified and analyzed ..
  47. ncbi Interactions of the Escherichia coli hydrogenase biosynthetic proteins: HybG complex formation
    Gareth Butland
    Banting and Best Department of Medical Research, University of Toronto, Toronto, Ont, Canada M5G 1L6
    FEBS Lett 580:677-81. 2006
    Assembly of the active site of the [NiFe]-hydrogenase enzymes involves a multi-step pathway and the coordinated activity of many accessory proteins...
  48. pmc Engineering hyperthermophilic archaeon Pyrococcus furiosus to overproduce its cytoplasmic [NiFe]-hydrogenase
    Sanjeev K Chandrayan
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 287:3257-64. 2012
    The cytoplasmic hydrogenase (SHI) of the hyperthermophilic archaeon Pyrococcus furiosus is an NADP(H)-dependent heterotetrameric enzyme that contains a nickel-iron catalytic site, flavin, and six iron-sulfur clusters...
  49. ncbi The hydrogenases and formate dehydrogenases of Escherichia coli
    G Sawers
    Lehrstuhl fur Mikrobiologie der Universitat Munchen, Germany
    Antonie Van Leeuwenhoek 66:57-88. 1994
    Escherichia coli has the capacity to synthesise three distinct formate dehydrogenase isoenzymes and three hydrogenase isoenzymes...
  50. pmc Role of the NiFe hydrogenase Hya in oxidative stress defense in Geobacter sulfurreducens
    Pier Luc Tremblay
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA
    J Bacteriol 194:2248-53. 2012
    ..Hyb, a periplasmically oriented membrane-bound NiFe hydrogenase, is essential for hydrogen-dependent growth. The functions of the three other hydrogenases are unknown...
  51. pmc Metabolic deficiences revealed in the biotechnologically important model bacterium Escherichia coli BL21(DE3)
    Constanze Pinske
    Institute for Microbiology, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    PLoS ONE 6:e22830. 2011
    ..hydrogen evolution was due solely to reduced activity of the formate dehydrogenase (FDH-H), not the hydrogenase component...
  52. ncbi The [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough is a bacterial lipoprotein lacking a typical lipoprotein signal peptide
    Filipa M A Valente
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, 1781 901 Oeiras, Portugal
    FEBS Lett 581:3341-4. 2007
    Desulfovibrio vulgaris Hildenborough has a membrane-bound [NiFeSe] hydrogenase whose mode of membrane association was unknown since it is constituted by two hydrophilic subunits...
  53. ncbi Metal binding activity of the Escherichia coli hydrogenase maturation factor HypB
    Michael R Leach
    Department of Chemistry, University of Toronto, Toronto, Ontario, Canada M5S 3H6
    Biochemistry 44:12229-38. 2005
    The formation of the [NiFe] metallocenter of Escherichia coli hydrogenase 3 requires the participation of proteins encoded by the hydrogenase pleiotropy operon hypABCDEF...
  54. ncbi Electrochemical kinetic investigations of the reactions of [FeFe]-hydrogenases with carbon monoxide and oxygen: comparing the importance of gas tunnels and active-site electronic/redox effects
    Gabrielle Goldet
    Inorganic Chemistry Laboratory, Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QR, United Kingdom
    J Am Chem Soc 131:14979-89. 2009
    ....
  55. doi Structural and biological analysis of the metal sites of Escherichia coli hydrogenase accessory protein HypB
    Alistair V Dias
    Department of Chemistry, University of Toronto, Toronto, Ontario, Canada
    Biochemistry 47:11981-91. 2008
    The [NiFe]-hydrogenase protein produced by many types of bacteria contains a dinuclear metal center that is required for enzymatic activity...
  56. pmc O2 reactions at the six-iron active site (H-cluster) in [FeFe]-hydrogenase
    Camilla Lambertz
    Lehrstuhl für Biochemie der Pflanzen, AG Photobiotechnologie, Ruhr Universitat Bochum, 44780 Bochum, Germany
    J Biol Chem 286:40614-23. 2011
    ..This mechanism suggests that blocking of ROS diffusion paths and/or altering the redox potential of the [4Fe4S] cubane by genetic engineering may yield improved O(2) tolerance in [FeFe]-hydrogenase.
  57. doi Development of a cell-free system reveals an oxygen-labile step in the maturation of [NiFe]-hydrogenase 2 of Escherichia coli
    Basem Soboh
    Institute for Mikrobiologie, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    FEBS Lett 584:4109-14. 2010
    ..of hydrogenases 1, 2 and 3 we could reconstitute in vitro under strictly anaerobic conditions 10-15% of the hydrogenase activity present in wild type Escherichia coli extracts...
  58. ncbi Biodiversity of uptake hydrogenase systems from legume endosymbiotic bacteria
    B Brito
    Laboratorio de Microbiologia, E T S Ingenieros Agronomos, Universidad Politecnica de Madrid, Ciudad Universitaria s n, 28040 Madrid, Spain
    Biochem Soc Trans 33:33-5. 2005
    ..Characterization of hydrogenase gene clusters in strains belonging to Rhizobium, Bradyrhizobium and Azorhizobium reveals a similar overall ..
  59. ncbi Crystallographic and FTIR spectroscopic evidence of changes in Fe coordination upon reduction of the active site of the Fe-only hydrogenase from Desulfovibrio desulfuricans
    Y Nicolet
    Laboratoire de Cristallographie et Cristallogenese des Proteines, Institut de Biologie Structurale, J P Ebel CEA CNRS, 41, rue Jules Horowitz, 38027 Grenoble, Cedex 1, France
    J Am Chem Soc 123:1596-601. 2001
    ..FTIR experiments performed on the Fe-only hydrogenase from Desulfovibrio desulfuricans indicate that upon reduction of the active oxidized form, there is a major ..
  60. doi Oxygen-tolerant [NiFe]-hydrogenases: the individual and collective importance of supernumerary cysteines at the proximal Fe-S cluster
    Michael J Lukey
    Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QR, UK
    J Am Chem Soc 133:16881-92. 2011
    ..Site-directed mutagenesis has been carried out on Escherichia coli hydrogenase-1 to substitute these cysteines (C19 and C120) individually and collectively for glycines, and the effects of ..
  61. pmc A bacterial electron-bifurcating hydrogenase
    Kai Schuchmann
    Department of Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe University Frankfurt Main, Max von Laue Strasse 9, 60438 Frankfurt, Germany
    J Biol Chem 287:31165-71. 2012
    ..We have purified a multimeric [FeFe]-hydrogenase from A...
  62. pmc How oxygen attacks [FeFe] hydrogenases from photosynthetic organisms
    Sven T Stripp
    Lehrstuhl Biochemie der Pflanzen, AG Photobiotechnologie, Ruhr Universitat Bochum, Universitatsstrasse 150, 44801 Bochum, Germany
    Proc Natl Acad Sci U S A 106:17331-6. 2009
    Green algae such as Chlamydomonas reinhardtii synthesize an [FeFe] hydrogenase that is highly active in hydrogen evolution...
  63. doi Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase
    Pierre Pol Liebgott
    Centre National de la Recherche Scientifique, Unité Propre de Recherche 9036, Unite de Bioenergetique et Ingenierie des Proteines, Institut Fédératif de Recherche 88, Institut de Microbiologie de la Mediterranee, Marseille, France
    Nat Chem Biol 6:63-70. 2010
    ..protein film voltammetry, we determined and then compared the rates of inhibition by CO and O2 in ten NiFe hydrogenase mutants and two FeFe hydrogenases...
  64. pmc A new activity for an old enzyme: Escherichia coli bacterial alkaline phosphatase is a phosphite-dependent hydrogenase
    Kechao Yang
    Department of Microbiology, University of Illinois at Urbana Champaign, B103 Chemical and Life Sciences Laboratory, 601 South Goodwin Avenue, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 101:7919-24. 2004
    ..Thus, BAP is a unique Pt-dependent, H2-evolving hydrogenase. This reaction is unprecedented in both P and H biochemistry, and it is likely to involve direct transfer of ..
  65. ncbi Hydrogenases from methanogenic archaea, nickel, a novel cofactor, and H2 storage
    Rudolf K Thauer
    Max Planck Institute for Terrestrial Microbiology, D 35043 Marburg, Germany
    Annu Rev Biochem 79:507-36. 2010
    ..NiFe]-hydrogenases, namely energy-converting [NiFe]-hydrogenases, heterodisulfide reductase-associated [NiFe]-hydrogenase or methanophenazine-reducing [NiFe]-hydrogenase, and F(420)-reducing [NiFe]-hydrogenase...
  66. ncbi Requirement of hydD, hydE, hypC and hypE genes for hydrogenase activity in Helicobacter pylori
    Stephane Benoit
    Department of Microbiology, 812 Biological Sciences Building, University of Georgia, Athens, GA 30602, USA
    Microb Pathog 36:153-7. 2004
    Helicobacter pylori possesses a membrane-bound, nickel containing, hydrogen uptake hydrogenase enzyme; its synthesis requires structural as well as accessory proteins, the latter needed for the complete maturation of the enzyme...
  67. ncbi Crystal structures of [NiFe] hydrogenase maturation proteins HypC, HypD, and HypE: insights into cyanation reaction by thiol redox signaling
    Satoshi Watanabe
    Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Mol Cell 27:29-40. 2007
    NiFe] hydrogenase maturation proteins HypC, HypD, and HypE catalyze the insertion and cyanation of the iron center of [NiFe] hydrogenases by an unknown mechanism...
  68. pmc Characterization of the oxygen tolerance of a hydrogenase linked to a carbon monoxide oxidation pathway in Rubrivivax gelatinosus
    Pin Ching Maness
    The National Renewable Energy Laboratory, Golden, Colorado 80401 3393, USA
    Appl Environ Microbiol 68:2633-6. 2002
    A hydrogenase linked to the carbon monoxide oxidation pathway in Rubrivivax gelatinosus displays tolerance to O2...
  69. doi Introduction of methionines in the gas channel makes [NiFe] hydrogenase aero-tolerant
    Sebastien Dementin
    CNRS, Bioenergetique et Ingenierie des Proteines, IMM, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 131:10156-64. 2009
    ..Improving hydrogenase O(2) tolerance is therefore a major contemporary challenge to allow the implementation of a sustainable ..
  70. ncbi Stepwise [FeFe]-hydrogenase H-cluster assembly revealed in the structure of HydA(DeltaEFG)
    David W Mulder
    Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, Montana 59717, USA
    Nature 465:248-51. 2010
    ..The catalytic core of the [FeFe]-hydrogenase (HydA), termed the H-cluster, exists as a [4Fe-4S] subcluster linked by a cysteine thiolate to a modified 2Fe ..
  71. pmc Optimization of metabolic capacity and flux through environmental cues to maximize hydrogen production by the cyanobacterium "Arthrospira (Spirulina) maxima"
    Gennady Ananyev
    Department of Chemistry, Princeton University, Hoyt Laboratory, Princeton, NJ 08544, USA
    Appl Environ Microbiol 74:6102-13. 2008
    ..by bacteria: (i) anaerobic H(2) production in the dark from whole cells catalyzed by a bidirectional [NiFe] hydrogenase is demonstrated to occur in two temporal phases involving two distinct metabolic processes that are linked to ..
  72. ncbi Theoretical study of dioxygen induced inhibition of [FeFe]-hydrogenase
    Martin T Stiebritz
    Laboratorium fur Physikalische Chemie, ETH Zurich, Wolfgang Pauli Strasse 10 8093 Zürich, Switzerland
    Inorg Chem 48:7127-40. 2009
    ..Out of this group, [FeFe]-hydrogenase shows the highest activity for hydrogen production which is, therefore, of great interest in the field of ..
  73. doi Metabolic engineering for solvent productivity by downregulation of the hydrogenase gene cluster hupCBA in Clostridium saccharoperbutylacetonicum strain N1-4
    Shun ichi Nakayama
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6 10 1, Fukuoka, 812 8581, Japan
    Appl Microbiol Biotechnol 78:483-93. 2008
    ..We have manipulated the activity level of a hydrogenase for this purpose because hydrogen and solvent production are closely correlated with each other...
  74. ncbi Unusual ligand structure in Ni-Fe active center and an additional Mg site in hydrogenase revealed by high resolution X-ray structure analysis
    Y Higuchi
    Division of Chemistry, Graduate School of Science, Kyoto University, Japan
    Structure 5:1671-80. 1997
    The hydrogenase of Desulfovibrio sp. catalyzes the reversible oxidoreduction of molecular hydrogen, in conjunction with a specific electron acceptor, cytochrome c3...
  75. ncbi GTP hydrolysis by HypB is essential for nickel insertion into hydrogenases of Escherichia coli
    T Maier
    Lehrstuhl fur Mikrobiologie, Universitat Munchen, Germany
    Eur J Biochem 230:133-8. 1995
    ..BKN117 did not display hydrogenase activity due to a loss of nickel incorporation into the large subunit...
  76. pmc Characterization of Helicobacter pylori nickel metabolism accessory proteins needed for maturation of both urease and hydrogenase
    Nalini Mehta
    Department of Microbiology, 527 Biological Sciences Building, University of Georgia, Athens, GA 30602, USA
    J Bacteriol 185:726-34. 2003
    ..demonstrated that two accessory proteins, HypA and HypB, play a role in nickel-dependent maturation of both hydrogenase and urease in Helicobacter pylori. Here, the two proteins were purified and characterized...
  77. pmc Gene products of the hupGHIJ operon are involved in maturation of the iron-sulfur subunit of the [NiFe] hydrogenase from Rhizobium leguminosarum bv. viciae
    Hamid Manyani
    Laboratorio de Microbiologia, Departamento de Biotecnologia, Escuela Tecnica Superior de Ingenieros Agronomos, Universidad Politecnica de Madrid, Ciudad Universitaria s n, 28040 Madrid, Spain
    J Bacteriol 187:7018-26. 2005
    In the present study, we investigate the functions of the hupGHIJ operon in the synthesis of an active [NiFe] hydrogenase in the legume endosymbiont Rhizobium leguminosarum bv. viciae...
  78. ncbi The complex between hydrogenase-maturation proteins HypC and HypD is an intermediate in the supply of cyanide to the active site iron of [NiFe]-hydrogenases
    Melanie Blokesch
    Department I der Fakultät für Biologie, Ludwig Maximilians Universitat Munchen, Maria Ward Strasse 1a, D 80638 Munich, Germany
    J Mol Biol 344:155-67. 2004
    ..In the absence of carbamoylphosphate, cells accumulate a complex of two hydrogenase maturation proteins, namely HypC and HypD for the synthesis of hydrogenase 3...
  79. ncbi [NiFe]-hydrogenases of Ralstonia eutropha H16: modular enzymes for oxygen-tolerant biological hydrogen oxidation
    Tanja Burgdorf
    Institut fur Biologie, Humboldt Universitat zu Berlin, Berlin, Germany
    J Mol Microbiol Biotechnol 10:181-96. 2005
    ..it produces three distinct [NiFe]-hydrogenases that each serve unique physiological roles: a membrane-bound hydrogenase (MBH) coupled to the respiratory chain, a cytoplasmic, soluble hydrogenase (SH) able to generate reducing ..
  80. pmc Selenium is involved in regulation of periplasmic hydrogenase gene expression in Desulfovibrio vulgaris Hildenborough
    Filipa M A Valente
    Instituto de Tecnologia Quimica e Biologica, Apt 127, 2781 901 Oeiras, Portugal
    J Bacteriol 188:3228-35. 2006
    ..which led to a strong repression of the [FeFe] and [NiFe]1 hydrogenases and a strong increase in the [NiFeSe] hydrogenase that is not detected in the absence of Se...
  81. ncbi Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli
    D J Richard
    Department of Biochemistry, University of Dundee, Tayside, UK
    Microbiology 145:2903-12. 1999
    ..The hydrogenase 1 and hydrogenase 2 enzymes are encoded by the polycistronic hyaABCDEF and hybOABCDEFG operons, respectively...
  82. ncbi Mechanism of regulation of the formate-hydrogenlyase pathway by oxygen, nitrate, and pH: definition of the formate regulon
    R Rossmann
    Lehrstuhl für Mikrobiologie der Universität, Munchen, Germany
    Mol Microbiol 5:2807-14. 1991
    ....
  83. pmc A cell-free microtiter plate screen for improved [FeFe] hydrogenases
    James A Stapleton
    Department of Chemical Engineering, Stanford University, Stanford, California, United States of America
    PLoS ONE 5:e10554. 2010
    FeFe] hydrogenase enzymes catalyze the production and dissociation of H(2), a potential renewable fuel...
  84. ncbi A multisubunit membrane-bound [NiFe] hydrogenase and an NADH-dependent Fe-only hydrogenase in the fermenting bacterium Thermoanaerobacter tengcongensis
    Basem Soboh
    Max Planck Institut fur terrestrische Mikrobiologie, Karl von Frisch Strasse, D 35043 Marburg, Germany
    Microbiology 150:2451-63. 2004
    ..A unique combination of hydrogenases, a ferredoxin-dependent [NiFe] hydrogenase and an NADH-dependent Fe-only hydrogenase, were found to be responsible for H(2) formation in this organism...
  85. ncbi Selective release and function of one of the two FMN groups in the cytoplasmic NAD+-reducing [NiFe]-hydrogenase from Ralstonia eutropha
    Eddy van der Linden
    Swammerdam Institute for Life Sciences, Biochemistry, University of Amsterdam, The Netherlands
    Eur J Biochem 271:801-8. 2004
    The soluble, cytoplasmic NAD+-reducing [NiFe]-hydrogenase from Ralstonia eutropha is a heterotetrameric enzyme (HoxFUYH) and contains two FMN groups...
  86. doi Photobiological hydrogen-producing systems
    Maria Lucia Ghirardi
    National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, USA
    Chem Soc Rev 38:52-61. 2009
    ....
  87. pmc Hydrogenase-3 contributes to anaerobic acid resistance of Escherichia coli
    Ken Noguchi
    Department of Biology, Kenyon College, Gambier, Ohio, United States of America
    PLoS ONE 5:e10132. 2010
    ..Because H(2) production involves consumption of 2H(+), hydrogenase expression is likely to involve pH response and regulation. Hydrogenase consumption of protons in E...
  88. doi Intermediates in the catalytic cycle of [NiFe] hydrogenase: functional spectroscopy of the active site
    Maria Eirini Pandelia
    Max Planck Institut fur Bioanorganische Chemie, Stiftstrasse 34 36, 45470, Mulheim an der Ruhr, Germany
    Chemphyschem 11:1127-40. 2010
    The [NiFe] hydrogenase from the anaerobic sulphate reducing bacterium Desulfovibrio vulgaris Miyazaki F is an excellent model for constructing a mechanism for the function of the so-called 'oxygen-sensitive' hydrogenases...
  89. ncbi The [Fe-Fe]-hydrogenase maturation protein HydF from Thermotoga maritima is a GTPase with an iron-sulfur cluster
    Xavier Brazzolotto
    Laboratoire de Chimie et Biochimie des Centres Redox Biologiques, UMR Université Joseph Fourier CNRS Commissariat à l Energie Atomique No 5047, Grenoble, France
    J Biol Chem 281:769-74. 2006
    ..HYSCORE spectroscopy experiments were carried out to determine the nature of the fourth ligand of the cluster, and its exchangeability was demonstrated with the formation of a [4Fe-4S]-imidazole complex...
  90. ncbi The soluble [NiFe]-hydrogenase from Ralstonia eutropha contains four cyanides in its active site, one of which is responsible for the insensitivity towards oxygen
    Eddy van der Linden
    Swammerdam Institute for Life Sciences, Biochemistry, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands
    J Biol Inorg Chem 9:616-26. 2004
    Infrared spectra of (15)N-enriched preparations of the soluble cytoplasmic NAD(+)-reducing [NiFe]-hydrogenase from Ralstonia eutropha are presented...
  91. ncbi Quantum refinement of [FeFe] hydrogenase indicates a dithiomethylamine ligand
    Ulf Ryde
    Theoretical Chemistry, Lund University, P O Box 124, SE 221 00 Lund, Sweden
    J Am Chem Soc 132:4512-3. 2010
    ..Thereby, we obtain structures that are an ideal compromise between DFT and crystallography...
  92. doi A unifying structural and electronic concept for Hmd and [FeFe] hydrogenase active sites
    Martin T Stiebritz
    Laboratorium fur Physikalische Chemie, ETH Zurich, Wolfgang Pauli Strasse 10, 8093 Zurich, Switzerland
    Inorg Chem 49:5818-23. 2010
    ..iron center of Hmd functions in a first-shell ligand environment resembling the reacting iron atom in [FeFe] hydrogenase and vice versa...
  93. ncbi Light-driven hydrogen production by a hybrid complex of a [NiFe]-hydrogenase and the cyanobacterial photosystem I
    Masaki Ihara
    Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Yokohama, Japan
    Photochem Photobiol 82:676-82. 2006
    ..In this study we engineered a 'hard-wired' protein complex consisting of a hydrogenase and photosystem I (hydrogenase-PSI complex) as a direct light-to-hydrogen conversion system...
  94. ncbi Structural and oxidation-state changes at its nonstandard Ni-Fe site during activation of the NAD-reducing hydrogenase from Ralstonia eutropha detected by X-ray absorption, EPR, and FTIR spectroscopy
    Tanja Burgdorf
    Humboldt Universitat zu Berlin, Mikrobiologie, Chausseestr 117, D 10115 Berlin, Germany
    J Am Chem Soc 127:576-92. 2005
    Structure and oxidation state of the Ni-Fe cofactor of the NAD-reducing soluble hydrogenase (SH) from Ralstonia eutropha were studied employing X-ray absorption spectroscopy (XAS) at the Ni K-edge, EPR, and FTIR spectroscopy...
  95. pmc Synthesis of the 2Fe subcluster of the [FeFe]-hydrogenase H cluster on the HydF scaffold
    Eric M Shepard
    Department of Chemistry and Biochemistry and the Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, MT 59717, USA
    Proc Natl Acad Sci U S A 107:10448-53. 2010
    The organometallic H cluster at the active site of [FeFe]-hydrogenase consists of a 2Fe subcluster coordinated by cyanide, carbon monoxide, and a nonprotein dithiolate bridged to a [4Fe-4S] cluster via a cysteinate ligand...
  96. pmc Rhizobium leguminosarum hupE encodes a nickel transporter required for hydrogenase activity
    Belen Brito
    Departamento de Biotecnologia, Escuela Tecnica Superior de Ingenieros Agronomos, Universidad Politecnica de Madrid, Madrid, Spain
    J Bacteriol 192:925-35. 2010
    ..we further characterize the nickel transport capacity of HupE and that of the translated product of hupE2, a hydrogenase-unlinked gene identified in the R. leguminosarum genome...
  97. pmc Respiratory hydrogen use by Salmonella enterica serovar Typhimurium is essential for virulence
    R J Maier
    Department of Microbiology, University of Georgia, Athens 30602, USA
    Infect Immun 72:6294-9. 2004
    ..enteric pathogen salmonella, like other enteric bacteria, contains three putative membrane-associated H2-using hydrogenase enzymes...
  98. doi Wiring an [FeFe]-hydrogenase with photosystem I for light-induced hydrogen production
    Carolyn E Lubner
    Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA
    Biochemistry 49:10264-6. 2010
    ..Photosystem I (PS I) and an [FeFe]-hydrogenase (H(2)ase) serve as the test bed for this new technology...
  99. ncbi Genetic and physiological characterization of new Escherichia coli mutants impaired in hydrogenase activity
    L F Wu
    Biochimie 68:167-79. 1986
    ..Three mutants were chosen for further analysis: they lacked hydrogenase (hydrogen: benzyl viologen oxidoreductase) activity, but produced normal levels of fumarate reductase activity ..
  100. ncbi A radical solution for the biosynthesis of the H-cluster of hydrogenase
    John W Peters
    Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA
    FEBS Lett 580:363-7. 2006
    ..Recently, it was discovered that radical S-adenosylmethionine enzymes were involved in active hydrogenase expression...
  101. doi The difference a Se makes? Oxygen-tolerant hydrogen production by the [NiFeSe]-hydrogenase from Desulfomicrobium baculatum
    Alison Parkin
    Inorganic Chemistry, University of Oxford, South Parks Road, OX1 3QR, England
    J Am Chem Soc 130:13410-6. 2008
    Protein film voltammetry studies of the [NiFeSe]-hydrogenase from Desulfomicrobium baculatum show it to be a highly efficient H2 cycling catalyst...

Research Grants69

  1. X-Ray Raman and Vibrational Mossbauer of Metalloproteins
    STEPHEN CRAMER; Fiscal Year: 2007
    ..Three of the enzyme active sites (NiFe hydrogenase, CO dehydrogenase, and acetyl-CoA synthase) contain combinations of Fe and Ni...
  2. National Biomedical EPR Center
    James Hyde; Fiscal Year: 2003
    ..Characterization of the iron-sulfur centers of the bi-directional hydrogenase Cpl from Clostridium pasteurianum...
  3. BIOLOGICAL METAL CLUSTERS--BIOPHYSICAL & MODEL STUDIES
    Michael Maroney; Fiscal Year: 1992
    ..g., nitrogenase-Mo, Fe; cytochrome oxidase-Fe, Cu; hydrogenase and carbon monoxide dehydrogenase-Ni, Fe), have been identified...
  4. BIOLOGICAL METAL CLUSTERS--BIOPHYSICAL AND MODEL STUDIES
    Michael Maroney; Fiscal Year: 1999
    ..g. superoxide dismutase, Cu, Zn; nitrogenase, Mo, Fe; cytochrome oxidase, heme a3, CuB; hydrogenase, acetyl coenzyme A synthase, carbon monoxide dehydrogenase, Ni, Fe)...
  5. BIOLOGICAL METAL CLUSTERS--BIOPHYSICAL AND MODEL STUDIES
    Michael Maroney; Fiscal Year: 1999
    ..g. superoxide dismutase, Cu, Zn; nitrogenase, Mo, Fe; cytochrome oxidase, heme a3, CuB; hydrogenase, acetyl coenzyme A synthase, carbon monoxide dehydrogenase, Ni, Fe)...
  6. MOLECULAR BASIS FOR FUNCTION OF IRON PROTEINS
    MARTIN KAMEN; Fiscal Year: 1980
    ..cytochromes c3 might substitute for Desulfovibrio cytochromes c3 as electron acceptors and effectors in the hydrogenase-catalyzed oxidation of hydrogen gas will be tested...
  7. CHIROPTICAL SPECTROSCOPY OF METALLOPROTEINS
    Philip Stephens; Fiscal Year: 1990
    ..Specific metalloproteins to be studied include the iron-sulfur (Fe-S) proteins: Desulfovibrio vulgaris hydrogenase, Klebsiella pneumoniae pyruvate-flavodoxin oxidoreductase, and Micrococcus aerogenes ferredoxin and the nickel-..
  8. SITE-SPECIFIC PROPERTIES OF A UNIQUE IRON-SULFUR PROTEIN
    Michael Adams; Fiscal Year: 1991
    ..These include nitrogenase, hydrogenase, CO dehydrogenase, aconitase, and various hydrolases...
  9. BIOLOGICAL METAL CLUSTERS--BIOPHYSICAL & MODEL STUDIES
    Michael Maroney; Fiscal Year: 1990
    ..Synthetic models for the hydrogenase and CO dehydrogenase active sites and proposed intermediates are defined, and the study of their spectroscopic ..
  10. X-RAY ABSORPTION SPECTROSCOPY OF METALLOENZYMES
    Robert Scott; Fiscal Year: 1991
    ..nickel-containing enzymes, including jackbean urease, Klebsiella aerogenes urease, Desulfovibrio gigas (NiFe) hydrogenase, D...
  11. X-RAY ABSORPTION SPECTROSCOPY OF METALLOENZYMES
    Robert Scott; Fiscal Year: 1993
    ..nickel-containing enzymes, including jackbean urease, Klebsiella aerogenes urease, Desulfovibrio gigas (NiFe) hydrogenase, D...
  12. X-RAY ABSORPTION SPECTROSCOPY OF METALLOENZYMES
    Robert Scott; Fiscal Year: 1992
    ..nickel-containing enzymes, including jackbean urease, Klebsiella aerogenes urease, Desulfovibrio gigas (NiFe) hydrogenase, D...
  13. X-RAY ABSORPTION SPECTROSCOPY OF METALLOENZYMES
    Robert Scott; Fiscal Year: 1990
    ..nickel-containing enzymes, including jackbean urease, Klebsiella aerogenes urease, Desulfovibrio gigas (NiFe) hydrogenase, D...
  14. MAGNETIZATION STUDIES OF METALLOPROTEINS
    EDMUND DAY; Fiscal Year: 1991
    ..more complex clusters including cytochrome c oxidase, sulfite and nitrite re- ductase, CO dehydrogenase, Ni hydrogenase, and nitrogenase will be inves- tigated...
  15. Cell Biology, Genetics, and Biochemistry of Giardia
    John Samuelson; Fiscal Year: 2003
    ..Giardia have an Fe-hydrogenase. These results suggest multiple hypotheses that will be tested in three Specific Aims...
  16. Cell Biology, Genetics, and Biochemistry of Giardia
    John Samuelson; Fiscal Year: 2006
    ..Giardia have an Fe-hydrogenase. These results suggest multiple hypotheses that will be tested in three Specific Aims...
  17. Cell Biology, Genetics, and Biochemistry of Giardia
    John Samuelson; Fiscal Year: 2007
    ..Giardia have an Fe-hydrogenase. These results suggest multiple hypotheses that will be tested in three Specific Aims...
  18. pH Regulation in Bacillus subtilis (AREA R15)
    JOAN SLONCZEWSKI; Fiscal Year: 2007
    ..pH regulation of hydrogenase enzymes has implications for the biological production of hydrogen fuel...
  19. pH Regulation in Bacillus subtilis (AREA R15)
    JOAN SLONCZEWSKI; Fiscal Year: 2009
    ..pH regulation of hydrogenase enzymes has implications for the biological production of hydrogen fuel...
  20. DEVELOPMENTAL GENOME REARRANGEMENT OF NIF GENES
    James Golden; Fiscal Year: 1999
    ..The excision of these elements is required for nitrogen fixation and uptake hydrogenase activity...
  21. DEVELOPMENTAL GENOME REARRANGEMENT OF NIF GENES
    James Golden; Fiscal Year: 2000
    ..The excision of these elements is required for nitrogen fixation and uptake hydrogenase activity...
  22. DEVELOPMENTAL GENOME REARRANGEMENT OF NIF GENES
    James Golden; Fiscal Year: 2002
    ..The excision of these elements is required for nitrogen fixation and uptake hydrogenase activity...
  23. DEVELOPMENTAL GENOME REARRANGEMENT OF NIF GENES
    James Golden; Fiscal Year: 2001
    ..The excision of these elements is required for nitrogen fixation and uptake hydrogenase activity...
  24. PHYSIOLOGY OF BACTERIAL NICKEL METABOLISM
    Robert Maier; Fiscal Year: 1990
    ..the routes of nickel binding, internalization, metabolism, and incorporation into a bacterial enzyme (hydrogenase) in the bacterium Bradyrhizobium japonicum. The specific aims are to: 1...
  25. DEVELOPMENTAL GENOME REARRANGEMENT OF NIF GENES
    James Golden; Fiscal Year: 2001
    ..The excision of these elements is required for nitrogen fixation and uptake hydrogenase activity...
  26. SOFT X-RAY SPECTROSCOPY OF METALLOPROTEINS
    STEPHEN CRAMER; Fiscal Year: 1991
    ..in the dioxygen-reducing enzyme cytochrome oxidase, and 3) the nickel and iron sites of the membrane bound hydrogenase from Azotobacter vinelandii...
  27. SOFT X-RAY SPECTROSCOPY OF METALLOPROTEINS
    STEPHEN CRAMER; Fiscal Year: 1992
    ..in the dioxygen-reducing enzyme cytochrome oxidase, and 3) the nickel and iron sites of the membrane bound hydrogenase from Azotobacter vinelandii...
  28. SOFT X-RAY SPECTROSCOPY OF METALLOPROTEINS
    STEPHEN CRAMER; Fiscal Year: 1990
    ..in the dioxygen-reducing enzyme cytochrome oxidase, and 3) the nickel and iron sites of the membrane bound hydrogenase from Azotobacter vinelandii...
  29. REGULATION OF H2 METABOLISM IN E COLI BY MOLYBDATE
    K Shanmugam; Fiscal Year: 1993
    Under anaerobic growth conditions, Escherichia coli produces hydrogenase and this enzyme activity is involved in H2 evolution (formate hydrogenlyase; FHL) and dihydrogen uptake...
  30. Development of a Novel Artificial Diiron Protein with N-hydroxylase Activity
    Marcos M Pires; Fiscal Year: 2010
    ..AurF is a recently discovered N-hydrogenase involved in the biosynthesis of the polyketide antibiotic aureothin...
  31. FE-S ENSEMBLES RELATED TO HYDROGENASE ACTIVITY
    THOMAS RAUCHFUSS; Fiscal Year: 2000
    The project goals are to develop molecular level understanding of the hydrogenase metalloenzymes, which effect the interconversion of H2 and H+...
  32. BIOLOGICALLY RELATED IRON SULFUR CHEMISTRY
    Richard Holm; Fiscal Year: 2000
    ..and cluster enlargement, as a route to uncharacterized biological clusters such as the Hclusters of hydrogenase; (11) utilization of high-nuclearity clusters as a means to the preparation and characterization of V-Fe-S and ..
  33. BIOLOGICALLY RELATED IRON SULFUR CHEMISTRY
    Richard Holm; Fiscal Year: 1999
    ..and cluster enlargement, as a route to uncharacterized biological clusters such as the Hclusters of hydrogenase; (11) utilization of high-nuclearity clusters as a means to the preparation and characterization of V-Fe-S and ..
  34. BIOLOGICAL FIXATION OF NITROGEN BY MICROORGANISMS
    ROBERT BURRIS; Fiscal Year: 1980
    The hydrogenase from Azotobacter vinelandii is being purified so that its properties can be compared with the hydrogenase recovered from soybean nodules...
  35. BIOLOGICALLY RELATED IRON/SULFUR CHEMISTRY
    Richard Holm; Fiscal Year: 1999
    ..and cluster enlargement, as a route to uncharacterized biological clusters such as the Hclusters of hydrogenase; (11) utilization of high-nuclearity clusters as a means to the preparation and characterization of V-Fe-S and ..
  36. BIOLOGICALLY RELATED IRON-SULFUR CHEMISTRY
    Richard Holm; Fiscal Year: 1991
    ..and cluster enlargement, as a route to uncharacterized biological clusters such as the Hclusters of hydrogenase; (11) utilization of high-nuclearity clusters as a means to the preparation and characterization of V-Fe-S and ..
  37. ASSESSING ROLES OF NICKEL HYDRIDES IN HYDROGENASES
    Marcetta Darensbourg; Fiscal Year: 1991
    ..of heterolytic dihydrogen cleavage/activation mechanisms as a model of the expected Ni(mu-SR)Fe sites in hydrogenase. An examination of hydride and small molecule binding by recently prepared is also proposed. (4...
  38. Synchrotron Spectroscopy of Nitrogenase and Hydrogenase
    Stephen P Cramer; Fiscal Year: 2010
    ..of two classes of Fe-based metalloenzymes with 'unusual'active sites - nitrogenase and hydrogenase. The questions that we hope to answer revolve around molecular structure (what atoms are where) dynamics (how ..
  39. MOSSBAUER AND EPR STUDIES OF HYDROGENASE
    Boi Hanh Huynh; Fiscal Year: 1990
    b>Hydrogenase are used by many micro-organisms, including algae, to metabolize H2. During the metabolic energy-yielding process, H2 is either oxidized or evolved as the product...
  40. Synchrotron Spectroscopy of Nitrogenase and Hydrogenase
    STEPHEN CRAMER; Fiscal Year: 2009
    ..of two classes of Fe-based metalloenzymes with 'unusual'active sites - nitrogenase and hydrogenase. The questions that we hope to answer revolve around molecular structure (what atoms are where) dynamics (how ..
  41. STUCTURE AND MECHANISM OF ACTION OF HYDROGENASE
    ALVIN KRASNA; Fiscal Year: 1980
    The aim of the proposed research is to elucidate the quaternary structure and mechanism of action of the enzyme hydrogenase. Pure hydrogenase from Chromatium, Proteus vulgaris, and Escherichia coli will be studied to establish the ..
  42. Synchrotron Spectroscopy of Nitrogenase and Hydrogenase
    Stephen P Cramer; Fiscal Year: 2011
    ..of two classes of Fe-based metalloenzymes with 'unusual'active sites - nitrogenase and hydrogenase. The questions that we hope to answer revolve around molecular structure (what atoms are where) dynamics (how ..
  43. Synchrotron Spectroscopy of Nitrogenase and Hydrogenase
    STEPHEN CRAMER; Fiscal Year: 2007
    ..of two classes of Fe-based metalloenzymes with 'unusual' active sites - nitrogenase and hydrogenase. The questions that we hope to answer revolve around molecular structure (what atoms are where) dynamics (how ..
  44. MODEL STUDIES OF ACETYL COENZYME A SYNTHASE
    Charles Riordan; Fiscal Year: 2005
    ..g. the catalytic sites in [NiFe] hydrogenase, sulfite reductase, cytochrome c oxidase, [CuZn] superoxide dismutase and the nitrogenase cofactor...
  45. MODEL STUDIES OF ACETYL COENZYME A SYNTHASE
    Charles Riordan; Fiscal Year: 2004
    ..g. the catalytic sites in [NiFe] hydrogenase, sulfite reductase, cytochrome c oxidase, [CuZn] superoxide dismutase and the nitrogenase cofactor...
  46. MODEL STUDIES OF ACETYL COENZYME A SYNTHASE
    Charles Riordan; Fiscal Year: 2000
    ..g. the catalytic sites in [NiFe] hydrogenase, sulfite reductase, cytochrome c oxidase, [CuZn] superoxide dismutase and the nitrogenase cofactor...
  47. MODEL STUDIES OF ACETYL COENZYME A SYNTHASE
    Charles Riordan; Fiscal Year: 2001
    ..g. the catalytic sites in [NiFe] hydrogenase, sulfite reductase, cytochrome c oxidase, [CuZn] superoxide dismutase and the nitrogenase cofactor...
  48. MODEL STUDIES OF ACETYL COENZYME A SYNTHASE
    Charles Riordan; Fiscal Year: 2003
    ..g. the catalytic sites in [NiFe] hydrogenase, sulfite reductase, cytochrome c oxidase, [CuZn] superoxide dismutase and the nitrogenase cofactor...
  49. DESULFOVIBRIO HYDROGENASE--MOLECULAR BIOLOGY
    HARRY PECK; Fiscal Year: 1991
    ..by specific probes utilizing biophysical techniques; 3) to attempt to isolate the gene product of the putative hydrogenase gene, hyd lambda from Desulfovibrio vulgaris which is suspected to be a new-type of (Fe) hydrogenase in ..
  50. MODEL STUDIES OF ACETYL COENZYME A SYNTHASE
    Charles Riordan; Fiscal Year: 2002
    ..g. the catalytic sites in [NiFe] hydrogenase, sulfite reductase, cytochrome c oxidase, [CuZn] superoxide dismutase and the nitrogenase cofactor...
  51. X-Ray Raman and Vibrational Mossbauer of Metalloproteins
    STEPHEN CRAMER; Fiscal Year: 2005
    ..Three of the enzyme active sites (NiFe hydrogenase, CO dehydrogenase, and acetyl-CoA synthase) contain combinations of Fe and Ni...
  52. X-Ray Raman and Vibrational Mossbauer of Metalloproteins
    STEPHEN CRAMER; Fiscal Year: 2002
    ..Three of the enzyme active sites (NiFe hydrogenase, CO dehydrogenase, and acetyl-CoA synthase) contain combinations of Fe and Ni...
  53. X-Ray Raman and Vibrational Mossbauer of Metalloproteins
    STEPHEN CRAMER; Fiscal Year: 2004
    ..Three of the enzyme active sites (NiFe hydrogenase, CO dehydrogenase, and acetyl-CoA synthase) contain combinations of Fe and Ni...
  54. X-Ray Raman and Vibrational Mossbauer of Metalloproteins
    STEPHEN CRAMER; Fiscal Year: 2003
    ..Three of the enzyme active sites (NiFe hydrogenase, CO dehydrogenase, and acetyl-CoA synthase) contain combinations of Fe and Ni...
  55. Virulence Determinants in Helicobacter hepaticus
    Robert Maier; Fiscal Year: 2003
    ..in 2003, and some suspected virulence factors (superoxide dismutase, alkyl hydroperoxide reductase, and hydrogenase) have been identified by enzyme assays on H. hepaticus extracts (in the Principal Investigator's lab)...