hydrogenase

Summary

Summary: An enzyme found in bacteria. It catalyzes the reduction of ferredoxin and other substances in the presence of molecular hydrogen and is involved in the electron transport of bacterial photosynthesis. EC 1.18.99.1.

Top Publications

  1. ncbi Occurrence, classification, and biological function of hydrogenases: an overview
    Paulette M Vignais
    CEA Grenoble, Laboratoire de Biochimie et Biophysique des Systemes Integres, UMR CEA CNRS UJF 5092, Institut de Recherches en Technologies et Sciences pour le Vivant iRTSV, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
    Chem Rev 107:4206-72. 2007
  2. ncbi The crystal structure of an oxygen-tolerant hydrogenase uncovers a novel iron-sulphur centre
    Johannes Fritsch
    Mikrobiologie, Institut fur Biologie, Humboldt Universitat zu Berlin, Chausseestraße 117, 10115 Berlin, Germany
    Nature 479:249-52. 2011
  3. ncbi The [NiFe]-hydrogenase of the cyanobacterium Synechocystis sp. PCC 6803 works bidirectionally with a bias to H2 production
    Chelsea L McIntosh
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA
    J Am Chem Soc 133:11308-19. 2011
  4. pmc Fe-hydrogenase maturases in the hydrogenosomes of Trichomonas vaginalis
    Simone Pütz
    Institut für Botanik III, Universitatsstrasse 1, 40225 Dusseldorf, Germany
    Eukaryot Cell 5:579-86. 2006
  5. ncbi Understanding and tuning the catalytic bias of hydrogenase
    Abbas Abou Hamdan
    Laboratoire de Bioenergetique et Ingenierie des Proteines, Institut de Microbiologie de la Mediterranee, CNRS and Aix Marseille Université, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 134:8368-71. 2012
  6. ncbi Crystal structure of the O(2)-tolerant membrane-bound hydrogenase 1 from Escherichia coli in complex with its cognate cytochrome b
    Anne Volbeda
    Metalloproteins Unit, Institut de Biologie Structurale J P Ebel CEA CNRS Université Joseph Fourier, UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble, France
    Structure 21:184-90. 2013
  7. ncbi Hydrogenase activity in the foodborne pathogen Campylobacter jejuni depends upon a novel ABC-type nickel transporter (NikZYXWV) and is SlyD-independent
    Robert M Howlett
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, UK
    Microbiology 158:1645-55. 2012
  8. pmc The iron-hydrogenase of Thermotoga maritima utilizes ferredoxin and NADH synergistically: a new perspective on anaerobic hydrogen production
    Gerrit J Schut
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602 7229, USA
    J Bacteriol 191:4451-7. 2009
  9. ncbi Structural basis for a [4Fe-3S] cluster in the oxygen-tolerant membrane-bound [NiFe]-hydrogenase
    Yasuhito Shomura
    Department of Life Science, Graduate School of Life Science, University of Hyogo, 3 2 1 Koto, Kamigori cho, Ako gun, Hyogo 678 1297, Japan
    Nature 479:253-6. 2011
  10. ncbi RNA silencing of hydrogenase(-like) genes and investigation of their physiological roles in the green alga Chlamydomonas reinhardtii
    James E Godman
    Department of Plant Sciences, University of Cambridge, UK
    Biochem J 431:345-51. 2010

Detail Information

Publications350 found, 100 shown here

  1. ncbi Occurrence, classification, and biological function of hydrogenases: an overview
    Paulette M Vignais
    CEA Grenoble, Laboratoire de Biochimie et Biophysique des Systemes Integres, UMR CEA CNRS UJF 5092, Institut de Recherches en Technologies et Sciences pour le Vivant iRTSV, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
    Chem Rev 107:4206-72. 2007
  2. ncbi The crystal structure of an oxygen-tolerant hydrogenase uncovers a novel iron-sulphur centre
    Johannes Fritsch
    Mikrobiologie, Institut fur Biologie, Humboldt Universitat zu Berlin, Chausseestraße 117, 10115 Berlin, Germany
    Nature 479:249-52. 2011
    ..Here we present the crystal structure of an O(2)-tolerant [NiFe]-hydrogenase from the aerobic H(2) oxidizer Ralstonia eutropha H16 at 1.5 Å resolution...
  3. ncbi The [NiFe]-hydrogenase of the cyanobacterium Synechocystis sp. PCC 6803 works bidirectionally with a bias to H2 production
    Chelsea L McIntosh
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA
    J Am Chem Soc 133:11308-19. 2011
    ..PFE) was utilized to characterize the catalytic activity and oxidative inactivation of a bidirectional [NiFe]-hydrogenase (HoxEFUYH) from the cyanobacterium Synechocystis sp. PCC 6803...
  4. pmc Fe-hydrogenase maturases in the hydrogenosomes of Trichomonas vaginalis
    Simone Pütz
    Institut für Botanik III, Universitatsstrasse 1, 40225 Dusseldorf, Germany
    Eukaryot Cell 5:579-86. 2006
    Assembly of active Fe-hydrogenase in the chloroplasts of the green alga Chlamydomonas reinhardtii requires auxiliary maturases, the S-adenosylmethionine-dependent enzymes HydG and HydE and the GTPase HydF...
  5. ncbi Understanding and tuning the catalytic bias of hydrogenase
    Abbas Abou Hamdan
    Laboratoire de Bioenergetique et Ingenierie des Proteines, Institut de Microbiologie de la Mediterranee, CNRS and Aix Marseille Université, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 134:8368-71. 2012
    ..Here, we focus on NiFe hydrogenase, an enzyme that catalyzes H(2) oxidation and production, and we elucidate the mechanism that governs the ..
  6. ncbi Crystal structure of the O(2)-tolerant membrane-bound hydrogenase 1 from Escherichia coli in complex with its cognate cytochrome b
    Anne Volbeda
    Metalloproteins Unit, Institut de Biologie Structurale J P Ebel CEA CNRS Université Joseph Fourier, UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble, France
    Structure 21:184-90. 2013
    We report the 3.3 Å resolution structure of dimeric membrane-bound O(2)-tolerant hydrogenase 1 from Escherichia coli in a 2:1 complex with its physiological partner, cytochrome b...
  7. ncbi Hydrogenase activity in the foodborne pathogen Campylobacter jejuni depends upon a novel ABC-type nickel transporter (NikZYXWV) and is SlyD-independent
    Robert M Howlett
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, UK
    Microbiology 158:1645-55. 2012
    ..The bacterium possesses a single membrane-bound, periplasmic-facing NiFe uptake hydrogenase that depends on the acquisition of environmental nickel for activity...
  8. pmc The iron-hydrogenase of Thermotoga maritima utilizes ferredoxin and NADH synergistically: a new perspective on anaerobic hydrogen production
    Gerrit J Schut
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602 7229, USA
    J Bacteriol 191:4451-7. 2009
    ..The trimeric [FeFe]-type hydrogenase that was previously purified from T...
  9. ncbi Structural basis for a [4Fe-3S] cluster in the oxygen-tolerant membrane-bound [NiFe]-hydrogenase
    Yasuhito Shomura
    Department of Life Science, Graduate School of Life Science, University of Hyogo, 3 2 1 Koto, Kamigori cho, Ako gun, Hyogo 678 1297, Japan
    Nature 479:253-6. 2011
    Membrane-bound respiratory [NiFe]-hydrogenase (MBH), a H(2)-uptake enzyme found in the periplasmic space of bacteria, catalyses the oxidation of dihydrogen: H(2) → 2H(+) + 2e(-) (ref. 1)...
  10. ncbi RNA silencing of hydrogenase(-like) genes and investigation of their physiological roles in the green alga Chlamydomonas reinhardtii
    James E Godman
    Department of Plant Sciences, University of Cambridge, UK
    Biochem J 431:345-51. 2010
    The genome of the green alga Chlamydomonas reinhardtii encodes two [FeFe]-hydrogenases, HydA1 and HydA2, and the hydrogenase-like protein HYD3...
  11. ncbi Production of biohydrogen by heterologous expression of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli
    Jaoon Y H Kim
    Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790 784, Republic of Korea
    J Biotechnol 155:312-9. 2011
    Oxygen sensitivity of hydrogenase is a critical issue in efficient biological hydrogen production...
  12. pmc Identification of the [FeFe]-hydrogenase responsible for hydrogen generation in Thermoanaerobacterium saccharolyticum and demonstration of increased ethanol yield via hydrogenase knockout
    A Joe Shaw
    Dartmouth College, Thayer School of Engineering, 8000 Cummings Hall, Hanover, NH 03755, USA
    J Bacteriol 191:6457-64. 2009
    Three putative hydrogenase enzyme systems in Thermoanaerobacterium saccharolyticum were investigated at the genetic, mRNA, enzymatic, and phenotypic levels...
  13. pmc Heterologous expression and maturation of an NADP-dependent [NiFe]-hydrogenase: a key enzyme in biofuel production
    Junsong Sun
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 5:e10526. 2010
    ..Microbial hydrogen production is catalyzed by hydrogenase, an extremely complex, air-sensitive enzyme that utilizes a binuclear nickel-iron [NiFe] catalytic site...
  14. ncbi Molecular hydrogen as an energy source for Helicobacter pylori
    Jonathan W Olson
    Department of Microbiology, University of Georgia, Athens, GA 30602, USA
    Science 298:1788-90. 2002
    ..pylori. Hydrogenase activity in H. pylori is constitutive but increased fivefold upon incubation with hydrogen...
  15. ncbi A unique iron-sulfur cluster is crucial for oxygen tolerance of a [NiFe]-hydrogenase
    Tobias Goris
    Institut für Biologie Mikrobiologie, Humboldt Universitat zu Berlin, Berlin, Germany
    Nat Chem Biol 7:310-8. 2011
    ..The O(2)-tolerant membrane-bound [NiFe]-hydrogenase of Ralstonia eutropha H16 is one of the few examples that can perform H(2) uptake in the presence of ambient O(..
  16. ncbi [FeFe]-hydrogenase in Yellowstone National Park: evidence for dispersal limitation and phylogenetic niche conservatism
    Eric S Boyd
    Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, 59717, USA
    ISME J 4:1485-95. 2010
    ..In this study, we examined the [FeFe]-hydrogenase gene (hydA) as a proxy for fermentative bacterial H₂ production along physical and chemical gradients in ..
  17. ncbi Oxygen-tolerant [NiFe]-hydrogenases: the individual and collective importance of supernumerary cysteines at the proximal Fe-S cluster
    Michael J Lukey
    Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QR, UK
    J Am Chem Soc 133:16881-92. 2011
    ..Site-directed mutagenesis has been carried out on Escherichia coli hydrogenase-1 to substitute these cysteines (C19 and C120) individually and collectively for glycines, and the effects of ..
  18. pmc Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii
    A Melis
    Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, Berkeley, California 94720 3102, USA
    Plant Physiol 122:127-36. 2000
    The work describes a novel approach for sustained photobiological production of H(2) gas via the reversible hydrogenase pathway in the green alga Chlamydomonas reinhardtii...
  19. ncbi Changing the ligation of the distal [4Fe4S] cluster in NiFe hydrogenase impairs inter- and intramolecular electron transfers
    Sebastien Dementin
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS UPR 9036, IBSM and Université de Provence, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Am Chem Soc 128:5209-18. 2006
    ..This demonstrates that although [4Fe4S] clusters are robust chemical constructs, the direct protein ligands play an essential role in imparting their ability to transfer electrons...
  20. ncbi Stepwise [FeFe]-hydrogenase H-cluster assembly revealed in the structure of HydA(DeltaEFG)
    David W Mulder
    Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, Montana 59717, USA
    Nature 465:248-51. 2010
    ..The catalytic core of the [FeFe]-hydrogenase (HydA), termed the H-cluster, exists as a [4Fe-4S] subcluster linked by a cysteine thiolate to a modified 2Fe ..
  21. ncbi Activation process of [NiFe] hydrogenase elucidated by high-resolution X-ray analyses: conversion of the ready to the unready state
    Hideaki Ogata
    Department of Life Science, Graduate School of Life Science, University of Hyogo and Himeji Institute of Technology, 3 2 1 Koto, Ako gun, Hyogo 678 1297, Japan
    Structure 13:1635-42. 2005
    ..NiFe] hydrogenase has two different oxidized states, Ni-A (unready, exhibits a lag phase in reductive activation) and Ni-B (ready)..
  22. ncbi Genomic analysis reveals multiple [FeFe] hydrogenases and hydrogen sensors encoded by treponemes from the H(2)-rich termite gut
    Nicholas R Ballor
    Biochemistry and Molecular Biophysics, California Institute of Technology, Pasadena, CA 91125, USA
    Microb Ecol 63:282-94. 2012
    ..The spirochetes encoded 4, 8, and 5 [FeFe] hydrogenase-like proteins, identified by their H domains, respectively, but no other recognizable hydrogenases...
  23. ncbi The three-dimensional structure of [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough: a hydrogenase without a bridging ligand in the active site in its oxidised, "as-isolated" state
    Marta C Marques
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Apartado 127, 2781 901 Oeiras, Portugal
    J Mol Biol 396:893-907. 2010
    ..This structure provides several insights that may explain the fast activation and O(2) tolerance of these enzymes...
  24. ncbi Use of molecular hydrogen as an energy substrate by human pathogenic bacteria
    R J Maier
    Department of Microbiology, University of Georgia, Athens, GA 30602, USA
    Biochem Soc Trans 33:83-5. 2005
    ..hepaticus) have the ability to use H(2) through a respiratory hydrogenase, and it was demonstrated that the gas is present in the tissues colonized by these pathogens (the stomach and ..
  25. ncbi Iron hydrogenases--ancient enzymes in modern eukaryotes
    David S Horner
    Dipartimento di Fisiologia e Biochimica Generali, University of Milan, Via Celoria 26, 20133, Milan, Italy
    Trends Biochem Sci 27:148-53. 2002
    ..These unique enzymes have thus moved from the margins of eukaryotic biology to become the focus of intense speculation and interest. This article summarizes current knowledge of their distribution, evolution and biochemistry...
  26. ncbi In vivo interactome of Helicobacter pylori urease revealed by tandem affinity purification
    Kerstin Stingl
    Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, 75015 Paris, France
    Mol Cell Proteomics 7:2429-41. 2008
    In the human gastric bacterium Helicobacter pylori, two metalloenzymes, hydrogenase and urease, are essential for in vivo colonization, the latter being a major virulence factor...
  27. ncbi Analysis of extensive [FeFe] hydrogenase gene diversity within the gut microbiota of insects representing five families of Dictyoptera
    Nicholas R Ballor
    Biochemistry and Molecular Biophysics, California Institute of Technology, M C 138 78, Pasadena, CA 91125, USA
    Microb Ecol 63:586-95. 2012
    We have designed and utilized degenerate primers in the phylogenetic analysis of [FeFe] hydrogenase gene diversity in the gut ecosystems of roaches and lower termites...
  28. ncbi Unusual ligand structure in Ni-Fe active center and an additional Mg site in hydrogenase revealed by high resolution X-ray structure analysis
    Y Higuchi
    Division of Chemistry, Graduate School of Science, Kyoto University, Japan
    Structure 5:1671-80. 1997
    The hydrogenase of Desulfovibrio sp. catalyzes the reversible oxidoreduction of molecular hydrogen, in conjunction with a specific electron acceptor, cytochrome c3...
  29. ncbi HypF, a carbamoyl phosphate-converting enzyme involved in [NiFe] hydrogenase maturation
    Athanasios Paschos
    Mikrobiologie, Department I der Fakultät für Biologie, Ludwig Maximilians Universitat Munchen, Maria Ward Strasse 1a, D 80638 Munich, Germany
    J Biol Chem 277:49945-51. 2002
    ..the derived amino acid sequence showed a full correlation of the resulting variants between their activity in hydrogenase maturation and the in vitro reactivity with carbamoyl phosphate...
  30. ncbi Efficient electron transfer from hydrogen to benzyl viologen by the [NiFe]-hydrogenases of Escherichia coli is dependent on the coexpression of the iron-sulfur cluster-containing small subunit
    Constanze Pinske
    Institute for Microbiology, Martin Luther University Halle Wittenberg, Kurt Mothes Str 3, 06120, Halle, Saale, Germany
    Arch Microbiol 193:893-903. 2011
    ..coli [NiFe]-hydrogenases restored activity of the respective hydrogenase to strain FTD147, which carries in-frame deletions in the hyaB, hybC, and hycE genes encoding the large ..
  31. ncbi The hows and whys of aerobic H2 metabolism
    Alison Parkin
    Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, Oxford OX1 3QR, UK
    Curr Opin Chem Biol 16:26-34. 2012
    ..In light of recent structural data and mutagenic studies this article reviews the molecular mechanism of O2-tolerance in [NiFe]-hydrogenases and discusses the biosynthesis of the unique Fe-S relay...
  32. pmc Distinct physiological roles of the three [NiFe]-hydrogenase orthologs in the hyperthermophilic archaeon Thermococcus kodakarensis
    Tamotsu Kanai
    Department of Biotechnology, College of Life Sciences, Ritsumeikan University, Kusatsu 525 8577, Japan
    J Bacteriol 193:3109-16. 2011
    ..with the reduction of elemental sulfur (S⁰) or H₂ generation, harbors three gene operons encoding [NiFe]-hydrogenase orthologs, namely, Hyh, Mbh, and Mbx...
  33. ncbi Structure/function relationships of [NiFe]- and [FeFe]-hydrogenases
    Juan C Fontecilla-Camps
    Laboratoire de Cristallographie et Cristallogenese des Proteines, Institut de Biologie Structurale J P Ebel, CEA, CNRS, Universite Joseph Fourier, 41 rue J Horowitz, 38027 Grenoble Cedex 1, France
    Chem Rev 107:4273-303. 2007
  34. ncbi Hydrogen fuel production by transgenic microalgae
    Anastasios Melis
    University of California, Department of Plant and Microbial Biology, 111 Koshland Hall, Berkeley, California 94720 3102, USA
    Adv Exp Med Biol 616:110-21. 2007
    ..Application of the green algal hydrogenase assembly genes is examined in efforts to confer H2-production capacity to other commercially significant ..
  35. ncbi The biosynthetic routes for carbon monoxide and cyanide in the Ni-Fe active site of hydrogenases are different
    Winfried Roseboom
    Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, NL 1018 WV Amsterdam, The Netherlands
    FEBS Lett 579:469-72. 2005
    ..The results suggest that the biosynthetic routes of the carbon monoxide and cyanide ligands in [NiFe]-hydrogenases are different...
  36. ncbi The hyperthermophilic bacterium, Thermotoga maritima, contains an unusually complex iron-hydrogenase: amino acid sequence analyses versus biochemical characterization
    M F Verhagen
    Department of Biochemistry and Molecular Biology and the Center for Metalloenzyme Studies, Life Sciences Building, The University of Georgia, Athens, GA, USA
    Biochim Biophys Acta 1412:212-29. 1999
    ..The H(2)-evolving cytoplasmic hydrogenase of this organism was shown to consist of three different subunits of masses 73 (alpha), 68 (beta) and 19 (gamma)..
  37. ncbi Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster
    J C Boyington
    Laboratory of Molecular Structure, National Institute of Allergy and Infectious Diseases, National Institutes of Health NIH, Rockville, MD 20852, USA
    Science 275:1305-8. 1997
    ..These structures suggest a reaction mechanism that directly involves SeCys140 and His141 in proton abstraction and the molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron transfer...
  38. ncbi Dithiomethylether as a ligand in the hydrogenase h-cluster
    Arti S Pandey
    Department of Chemistry and Biochemistry and Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, Montana 59717, USA
    J Am Chem Soc 130:4533-40. 2008
    An X-ray crystallographic refinement of the H-cluster of [FeFe]-hydrogenase from Clostridium pasteurianum has been carried out to close-to atomic resolution and is the highest resolution [FeFe]-hydrogenase presented to date. The 1...
  39. pmc Respiratory hydrogen use by Salmonella enterica serovar Typhimurium is essential for virulence
    R J Maier
    Department of Microbiology, University of Georgia, Athens 30602, USA
    Infect Immun 72:6294-9. 2004
    ..enteric pathogen salmonella, like other enteric bacteria, contains three putative membrane-associated H2-using hydrogenase enzymes...
  40. pmc Flexibility in anaerobic metabolism as revealed in a mutant of Chlamydomonas reinhardtii lacking hydrogenase activity
    Alexandra Dubini
    Environmental Science and Engineering Division, Colorado School of Mines, Golden, Colorado 80401, USA
    J Biol Chem 284:7201-13. 2009
    ..b>Hydrogenase activity is an important component of this metabolism, and we have compared metabolic and regulatory responses ..
  41. ncbi Hydrogenases: hydrogen-activating enzymes
    Michel Frey
    Laboratoire de Cristallographie et de Cristallogenese des Proteines, Institut de Biologie Structurale Jean Pierre Ebel, CEA CNRS, 41 rue Jules Horowitz, 38027 Grenoble, France
    Chembiochem 3:153-60. 2002
  42. ncbi Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe] hydrogenase
    Matthew C Posewitz
    National Renewable Energy Laboratory, Colorado School of Mines, Golden, CO 80401, USA
    J Biol Chem 279:25711-20. 2004
    ..One of the identified mutants, denoted hydEF-1, is incapable of assembling an active [Fe] hydrogenase. Although the hydEF-1 mutant transcribes both hydrogenase genes and accumulates full-length hydrogenase protein,..
  43. pmc Hydrogen production in Chlamydomonas: photosystem II-dependent and -independent pathways differ in their requirement for starch metabolism
    Vincent Chochois
    Commissariat a l Energie Atomique, Direction des Sciences du Vivant, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Commissariat à l Energie Atomique Cadarache, F 13108 Saint Paul lez Durance, France
    Plant Physiol 151:631-40. 2009
    ..In the direct pathway, photosystem II (PSII) supplies electrons to hydrogenase through the photosynthetic electron transport chain, while in the indirect pathway, hydrogen is produced in the ..
  44. ncbi Carbamoylphosphate serves as the source of CN(-), but not of the intrinsic CO in the active site of the regulatory [NiFe]-hydrogenase from Ralstonia eutropha
    Oliver Lenz
    Humboldt Universitat zu Berlin, Institut für Biologie Mikrobiologie, Chausseestrasse 117, Berlin, Germany
    FEBS Lett 581:3322-6. 2007
    ..To identify the metabolic origins of these ligands, the regulatory [NiFe] hydrogenase in conjunction with the indigenous Hyp maturation proteins of Ralstonia eutropha H16 were heterologously ..
  45. ncbi Investigating and exploiting the electrocatalytic properties of hydrogenases
    Kylie A Vincent
    Inorganic Chemistry Laboratory, Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QR, United Kingdom
    Chem Rev 107:4366-413. 2007
  46. ncbi Maturation of [NiFe]-hydrogenases in Escherichia coli
    Lucia Forzi
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
    Biometals 20:565-78. 2007
    ..in synthesis and attachment of these ligands to the iron atom, insertion of the Fe(CN)2CO group into the apo-hydrogenase, introduction of the nickel atom into the pre-formed active site and ensuring that the holoenzyme is correctly ..
  47. ncbi Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms
    Maria L Ghirardi
    National Renewable Energy Laboratory, Golden, CO 80401, USA
    Annu Rev Plant Biol 58:71-91. 2007
    ..In these organisms, photosynthetic water splitting is functionally linked to H(2) production by the activity of hydrogenase enzymes...
  48. pmc Salmonella enterica serovar Typhimurium NiFe uptake-type hydrogenases are differentially expressed in vivo
    Andrea L Zbell
    Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA
    Infect Immun 76:4445-54. 2008
    ..A recombination-based method to examine promoter activity in vivo (RIVET) was used to determine hydrogenase gene expression in macrophages, polymorphonuclear leukocyte (PMN)-like cells, and a mouse model of ..
  49. ncbi The difference a Se makes? Oxygen-tolerant hydrogen production by the [NiFeSe]-hydrogenase from Desulfomicrobium baculatum
    Alison Parkin
    Inorganic Chemistry, University of Oxford, South Parks Road, OX1 3QR, England
    J Am Chem Soc 130:13410-6. 2008
    Protein film voltammetry studies of the [NiFeSe]-hydrogenase from Desulfomicrobium baculatum show it to be a highly efficient H2 cycling catalyst...
  50. pmc Hydrogen production. Green algae as a source of energy
    A Melis
    Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720 3102, USA
    Plant Physiol 127:740-8. 2001
    ..Thus, processes that are presently conceptual in nature, or at a developmental stage in the laboratory, need to be encouraged, tested for feasibility, and otherwise applied toward commercialization...
  51. pmc How oxygen attacks [FeFe] hydrogenases from photosynthetic organisms
    Sven T Stripp
    Lehrstuhl Biochemie der Pflanzen, AG Photobiotechnologie, Ruhr Universitat Bochum, Universitatsstrasse 150, 44801 Bochum, Germany
    Proc Natl Acad Sci U S A 106:17331-6. 2009
    Green algae such as Chlamydomonas reinhardtii synthesize an [FeFe] hydrogenase that is highly active in hydrogen evolution...
  52. ncbi The photobiological production of hydrogen: potential efficiency and effectiveness as a renewable fuel
    Roger C Prince
    ExxonMobil Research and Engineering Co, Annandale, New Jersey 08801, USA
    Crit Rev Microbiol 31:19-31. 2005
    ....
  53. ncbi Construction and characterization of ack deleted mutant of Clostridium tyrobutyricum for enhanced butyric acid and hydrogen production
    Xiaoguang Liu
    Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Prog 22:1265-75. 2006
    ..that the AK activity in PAK-Em decreased by approximately 50%; meanwhile, phosphotransacetylase (PTA) and hydrogenase activities each increased by approximately 40%...
  54. ncbi Electrochemical definitions of O2 sensitivity and oxidative inactivation in hydrogenases
    Kylie A Vincent
    Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QR, UK
    J Am Chem Soc 127:18179-89. 2005
    ..Reactions with O2 vary greatly; the [FeFe]-hydrogenase from Desulfovibrio desulfuricans ATCC 7757, an anaerobe, is irreversibly damaged by O2, surviving only if ..
  55. pmc Rewiring hydrogenase-dependent redox circuits in cyanobacteria
    Daniel C Ducat
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 108:3941-6. 2011
    ..increasing the photobiological production of hydrogen within cyanobacterial cultures, we expressed the [FeFe] hydrogenase, HydA, from Clostridium acetobutylicum in the non-nitrogen-fixing cyanobacterium Synechococcus elongatus sp...
  56. ncbi The crystal structure of the [NiFe] hydrogenase from the photosynthetic bacterium Allochromatium vinosum: characterization of the oxidized enzyme (Ni-A state)
    Hideaki Ogata
    Max Planck Institut fur Bioanorganische Chemie, Stiftstrasse 34 36, D 45470 Mulheim an der Ruhr, Germany
    J Mol Biol 402:428-44. 2010
    The crystal structure of the membrane-associated [NiFe] hydrogenase from Allochromatium vinosum has been determined to 2.1 Å resolution...
  57. pmc The AbrB2 autorepressor, expressed from an atypical promoter, represses the hydrogenase operon to regulate hydrogen production in Synechocystis strain PCC6803
    Jérémy Dutheil
    UMR8221, CEA, CNRS, Universite Paris Sud, iBiTec S, LBBC, Gif sur Yvette, France
    J Bacteriol 194:5423-33. 2012
    ..Furthermore, we demonstrate that AbrB2 also represses the hox operon, which encodes the Ni-Fe hydrogenase of biotechnological interest, and that the hox operon is weakly expressed even though it possesses the two ..
  58. ncbi Purification and characterization of putative alkaline [Ni-Fe] hydrogenase from unicellular marine green alga, Tetraselmis kochinensis NCIM 1605
    S H Bhosale
    Biochemical Sciences Division, National Chemical Laboratory, Pune, Maharashtra, India
    Microbiol Res 164:131-7. 2009
    b>Hydrogenase enzyme from the unicellular marine green alga Tetraselmis kochinensis NCIM 1605 was purified 467 fold to homogeneity. The molecular weight was estimated to be approximately 89kDa by SDS-PAGE...
  59. pmc Essential anaplerotic role for the energy-converting hydrogenase Eha in hydrogenotrophic methanogenesis
    Thomas J Lie
    Department of Microbiology, University of Washington, Seattle WA 98195, USA
    Proc Natl Acad Sci U S A 109:15473-8. 2012
    ..However, an alternative hypothesis posits that the energy-converting hydrogenase Eha provides a chemiosmosis-driven electron input to the endergonic reaction...
  60. ncbi Engineering a non-native hydrogen production pathway into Escherichia coli via a cyanobacterial [NiFe] hydrogenase
    Mark A Wells
    ChELSI Institute, Department of Chemical and Biological Engineering, University of Sheffield, Mappin Street, UK
    Metab Eng 13:445-53. 2011
    ..Hydrogen production is observed in vivo in a hydrogenase null host, demonstrating coupling to host electron transfer systems...
  61. ncbi [NiFe]-hydrogenase maturation: isolation of a HypC-HypD complex carrying diatomic CO and CN- ligands
    Basem Soboh
    Institute of Microbiology, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    FEBS Lett 586:3882-7. 2012
    ..We prove that HypD cysteine 41 is required for the coordination of all three ligands. These findings suggest that the HypCD complex carries minimally the Fe(CN)(2)CO cofactor...
  62. pmc Hydrogen production by termite gut protists: characterization of iron hydrogenases of Parabasalian symbionts of the termite Coptotermes formosanus
    Jun ichi Inoue
    Environmental Molecular Biology Laboratory, RIKEN, Hirosawa 2 1, Wako, Saitama, Japan
    Eukaryot Cell 6:1925-32. 2007
    ..The results suggest that termite gut symbionts are a rich reservoir of novel Fe hydrogenases whose properties are adapted to the gut environment and that the potential of H(2) production in termite guts has been largely underestimated...
  63. pmc Genetic analysis of the Hox hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803 reveals subunit roles in association, assembly, maturation, and function
    Carrie Eckert
    Biosciences Center, National Renewable Energy Laboratory, Golden, Colorado 80401, USA
    J Biol Chem 287:43502-15. 2012
    Hydrogenases are metalloenzymes that catalyze 2H(+) + 2e(-) ↔ H(2). A multisubunit, bidirectional [NiFe]-hydrogenase has been identified and characterized in a number of bacteria, including cyanobacteria, where it is hypothesized to ..
  64. ncbi A central functional role for the 49-kDa subunit within the catalytic core of mitochondrial complex I
    N Kashani-Poor
    , , D-60590 Frankfurt am Main, Federal Republic of Germany
    J Biol Chem 276:24082-7. 2001
    ..The fact that the small subunit of hydrogenase is homologous to the PSST subunit of complex I proposed to host cluster N2 offers a straightforward explanation ..
  65. pmc Structural basis for the reaction mechanism of S-carbamoylation of HypE by HypF in the maturation of [NiFe]-hydrogenases
    Yasuhito Shomura
    Department of Life Science, Graduate School of Life Science, University of Hyogo, Hyogo 678 1297, Japan
    J Biol Chem 287:28409-19. 2012
    As a remarkable structural feature of hydrogenase active sites, [NiFe]-hydrogenases harbor one carbonyl and two cyano ligands, where HypE and HypF are involved in the biosynthesis of the nitrile group as a precursor of the cyano groups...
  66. ncbi Oriented immobilization of Desulfovibrio gigas hydrogenase onto carbon electrodes by covalent bonds for nonmediated oxidation of H2
    Olaf Rüdiger
    Instituto de Catalisis, CSIC, c Marie Curie 2, Cantoblanco, 28049 Madrid, Spain
    J Am Chem Soc 127:16008-9. 2005
    The orientation of hydrogenase bound covalently to a pyrolytic graphite edge electrode modified with a 4-aminophenyl monolayer can be modulated via electrostatic interactions during the immobilization step...
  67. ncbi Insights into [FeFe]-hydrogenase structure, mechanism, and maturation
    David W Mulder
    Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, MT 59717, USA
    Structure 19:1038-52. 2011
    ..The wealth of structural information on different classes and different states of hydrogenase enzymes, biosynthetic intermediates, and maturation enzymes has contributed significantly to understanding the ..
  68. pmc Genome data mining and soil survey for the novel group 5 [NiFe]-hydrogenase to explore the diversity and ecological importance of presumptive high-affinity H(2)-oxidizing bacteria
    Philippe Constant
    Max Planck Institute for Terrestrial Microbiology, Department of Biogeochemistry, Karl von Frisch Strasse 10, 35043 Marburg, Germany
    Appl Environ Microbiol 77:6027-35. 2011
    ..the unique ability to oxidize atmospheric H(2) possess genes specifying a putative high-affinity [NiFe]-hydrogenase. This study was undertaken to explore the taxonomic diversity and the ecological importance of this novel ..
  69. ncbi Photosynthetic hydrogen production by a hybrid complex of photosystem I and [NiFe]-hydrogenase
    Henning Krassen
    Department of Chemistry, Bielefeld University, D 33615 Bielefeld, Germany
    ACS Nano 3:4055-61. 2009
    ..step for conversion of solar energy into H(2) lies in the efficient electronic coupling of photosystem I and hydrogenase. Here we show the stepwise assembly of a hybrid complex consisting of photosystem I and hydrogenase on a solid ..
  70. ncbi O2-independent formation of the inactive states of NiFe hydrogenase
    Abbas Abou Hamdan
    Aix Marseille Universite, Centre National de la Recherche Scientifique CNRS, Laboratoire de Bioenergetique et Ingenierie des Proteines, Unité Mixte de Recherche 7281, Institut de Microbiologie de la Mediterranee, Marseille, France
    Nat Chem Biol 9:15-7. 2013
    We studied the mechanism of aerobic inactivation of Desulfovibrio fructosovorans nickel-iron (NiFe) hydrogenase by quantitatively examining the results of electrochemistry, EPR and FTIR experiments...
  71. ncbi Evolutionary significance of an algal gene encoding an [FeFe]-hydrogenase with F-domain homology and hydrogenase activity in Chlorella variabilis NC64A
    Jonathan E Meuser
    Division of Environmental Science and Engineering, Colorado School of Mines, Golden, CO 80401, USA
    Planta 234:829-43. 2011
    ..to Chlamydomonas reinhardtii, Chlorella variabilis NC64A (Trebouxiophyceae, Chlorophyta) exhibits [FeFe]-hydrogenase (HYDA) activity during anoxia. In contrast to C...
  72. pmc Overexpression, isolation, and spectroscopic characterization of the bidirectional [NiFe] hydrogenase from Synechocystis sp. PCC 6803
    Frauke Germer
    Botanisches Institut, Universitat Kiel, Am Botanischen Garten 1 9, D 24118 Kiel, Germany
    J Biol Chem 284:36462-72. 2009
    The bidirectional [NiFe] hydrogenase of the cyanobacterium Synechocystis sp...
  73. ncbi The role of the bidirectional hydrogenase in cyanobacteria
    Damian Carrieri
    Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA
    Bioresour Technol 102:8368-77. 2011
    ..Of the two cyanobacterial enzymes capable of evolving hydrogen gas (nitrogenase and the bidirectional hydrogenase), the hox-encoded bidirectional Ni-Fe hydrogenase has a high theoretical potential...
  74. ncbi Characterization of divergent NtrA-dependent promoters in the anaerobically expressed gene cluster coding for hydrogenase 3 components of Escherichia coli
    S Lutz
    Lehrstuhl fur Mikrobiologie, Universitat Munchen, FRG
    Mol Microbiol 4:13-20. 1990
    ..region of two divergently oriented transcriptional units involved in the formation of the gas-evolving hydrogenase (isoenzyme 3) of Escherichia coli was investigated...
  75. pmc Helicobacter pylori hydrogenase accessory protein HypA and urease accessory protein UreG compete with each other for UreE recognition
    Stéphane L Benoit
    Department of Microbiology, University of Georgia, 805 Biological Sciences Bldg, Athens, GA, USA
    Biochim Biophys Acta 1820:1519-25. 2012
    The gastric pathogen Helicobacter pylori relies on nickel-containing urease and hydrogenase enzymes in order to colonize the host...
  76. ncbi Hydrogenases in green algae: do they save the algae's life and solve our energy problems?
    Thomas Happe
    Botanisches Institut, Abt Molekulare Biochemie, Universitat Bonn, Karlrobert Kreiten Strasse 13, 53115 Bonn, Germany
    Trends Plant Sci 7:246-50. 2002
    ..The anaerobically inducible hydA genes of algae encode a special type of highly active [Fe]-hydrogenase. Electrons from reducing equivalents generated during fermentation enter the photosynthetic electron transport ..
  77. pmc X-ray crystallographic and computational studies of the O2-tolerant [NiFe]-hydrogenase 1 from Escherichia coli
    Anne Volbeda
    Metalloproteins Unit, Institut de Biologie Structurale J P Ebel, Commissariat à l Energie Atomique Centre National de la Recherche Scientifique l Université Joseph Fourier, 41 rue Jules Horowitz, 38027 Grenoble, France
    Proc Natl Acad Sci U S A 109:5305-10. 2012
    The crystal structure of the membrane-bound O(2)-tolerant [NiFe]-hydrogenase 1 from Escherichia coli (EcHyd-1) has been solved in three different states: as-isolated, H(2)-reduced, and chemically oxidized...
  78. pmc Hitherto unknown [Fe-Fe]-hydrogenase gene diversity in anaerobes and anoxic enrichments from a moderately acidic fen
    Oliver Schmidt
    Department of Ecological Microbiology, University of Bayreuth, 95445 Bayreuth, Germany
    Appl Environ Microbiol 76:2027-31. 2010
    ..Comparative analysis of [Fe-Fe]-hydrogenase and 16S rRNA gene-based phylogenies indicated the presence of homologous multiple hydrogenases per organism and ..
  79. ncbi Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath)
    R Csáki
    Department of Biotechnology, University of Szeged, Hungary
    FEMS Microbiol Lett 205:203-7. 2001
    The first gene cluster encoding for a membrane bound [NiFe] hydrogenase from a methanotroph, Methylococcus capsulatus (Bath), was cloned and sequenced...
  80. ncbi Sodium ion-dependent hydrogen production in Acidaminococcus fermentans
    U Härtel
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, D 35032 Marburg, Germany
    Arch Microbiol 166:350-6. 1996
    ..The acetate/butyrate ratio thereby increased from 2.0 at 1 mM Na+ to 3.0 at 100 mM Na+. A hydrogenase and an NADH dehydrogenase, both of which were detected in membrane fractions, are components of a model in ..
  81. ncbi The Escherichia coli metal-binding chaperone SlyD interacts with the large subunit of [NiFe]-hydrogenase 3
    Kim C Chan Chung
    Department of Chemistry, University of Toronto, Toronto, ON, Canada
    FEBS Lett 585:291-4. 2011
    The multi-step biosynthesis of the [NiFe]-hydrogenase enzyme involves a variety of accessory proteins...
  82. ncbi Alcohol dehydrogenase and hydrogenase transcript fluctuations during a day-night cycle in Chlamydomonas reinhardtii: the role of anoxia
    Larisa Angela Swirsky Whitney
    Department of Crop Plant Biology, University of Pisa, Pisa, Italy
    New Phytol 190:488-98. 2011
    ..The cell cycle and light-dark cycles are equally important elements in the regulatory network modulating the anaerobic response in Chlamydomonas...
  83. pmc Photosynthetic electron partitioning between [FeFe]-hydrogenase and ferredoxin:NADP+-oxidoreductase (FNR) enzymes in vitro
    Iftach Yacoby
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Proc Natl Acad Sci U S A 108:9396-401. 2011
    Photosynthetic water splitting, coupled to hydrogenase-catalyzed hydrogen production, is considered a promising clean, renewable source of energy...
  84. pmc Mechanism of proton transfer in [FeFe]-hydrogenase from Clostridium pasteurianum
    Adam J Cornish
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824 1319, USA
    J Biol Chem 286:38341-7. 2011
    ..Previously, structural characterization of [FeFe]-hydrogenase from Clostridium pasteurianum indicated a potential proton transport pathway involving four residues (Cys-299, ..
  85. ncbi A model system for [NiFe] hydrogenase maturation studies: Purification of an active site-containing hydrogenase large subunit without small subunit
    Gordon Winter
    Institut für Biologie Mikrobiologie, Humboldt Universitat zu Berlin, Chausseestr 117, D 10115 Berlin, Germany
    FEBS Lett 579:4292-6. 2005
    The large subunit HoxC of the H2-sensing [NiFe] hydrogenase from Ralstonia eutropha was purified without its small subunit. Two forms of HoxC were identified. Both forms contained iron but only substoichiometric amounts of nickel...
  86. pmc Engineering hyperthermophilic archaeon Pyrococcus furiosus to overproduce its cytoplasmic [NiFe]-hydrogenase
    Sanjeev K Chandrayan
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 287:3257-64. 2012
    The cytoplasmic hydrogenase (SHI) of the hyperthermophilic archaeon Pyrococcus furiosus is an NADP(H)-dependent heterotetrameric enzyme that contains a nickel-iron catalytic site, flavin, and six iron-sulfur clusters...
  87. pmc Control of hydrogen photoproduction by the proton gradient generated by cyclic electron flow in Chlamydomonas reinhardtii
    Dimitri Tolleter
    Commissariat à l Énergie Atomique et aux Énergies Alternatives, Direction des Sciences du Vivant, Institut de Biologie Environementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Commissariat à l Energie Atomique Cadarache, 13108 Saint Paul Lez Durance, France
    Plant Cell 23:2619-30. 2011
    ..microalgae results from a connection between the photosynthetic electron transport chain and a plastidial hydrogenase. Algal H₂ production is a transitory phenomenon under most natural conditions, often viewed as a safety valve ..
  88. ncbi Interactions of the Escherichia coli hydrogenase biosynthetic proteins: HybG complex formation
    Gareth Butland
    Banting and Best Department of Medical Research, University of Toronto, Toronto, Ont, Canada M5G 1L6
    FEBS Lett 580:677-81. 2006
    Assembly of the active site of the [NiFe]-hydrogenase enzymes involves a multi-step pathway and the coordinated activity of many accessory proteins...
  89. pmc Role of the NiFe hydrogenase Hya in oxidative stress defense in Geobacter sulfurreducens
    Pier Luc Tremblay
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA
    J Bacteriol 194:2248-53. 2012
    ..Hyb, a periplasmically oriented membrane-bound NiFe hydrogenase, is essential for hydrogen-dependent growth. The functions of the three other hydrogenases are unknown...
  90. ncbi The hydrogenases and formate dehydrogenases of Escherichia coli
    G Sawers
    Lehrstuhl fur Mikrobiologie der Universitat Munchen, Germany
    Antonie Van Leeuwenhoek 66:57-88. 1994
    Escherichia coli has the capacity to synthesise three distinct formate dehydrogenase isoenzymes and three hydrogenase isoenzymes...
  91. ncbi Heterologous expression of Alteromonas macleodii and Thiocapsa roseopersicina [NiFe] hydrogenases in Escherichia coli
    P D Weyman
    Department of Synthetic Biology and Bioenergy, The J Craig Venter Institute, Rockville, MD 20850, USA
    Microbiology 157:1363-74. 2011
    HynSL from Alteromonas macleodii 'deep ecotype' (AltDE) is an oxygen-tolerant and thermostable [NiFe] hydrogenase. Its two structural genes (hynSL), encoding small and large hydrogenase subunits, are surrounded by eight genes (hynD, hupH ..
  92. ncbi Growth- and substrate-dependent transcription of formate dehydrogenase and hydrogenase coding genes in Syntrophobacter fumaroxidans and Methanospirillum hungatei
    Petra Worm
    Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands
    Microbiology 157:280-9. 2011
    ..Our results strongly suggest that in syntrophically grown S. fumaroxidans cells, the [FeFe]-hydrogenase (encoded by Sfum_844-46), FDH1 (Sfum_2703-06) and Hox (Sfum_2713-16) may confurcate electrons from NADH and ..
  93. ncbi The CO and CN(-) ligands to the active site Fe in [NiFe]-hydrogenase of Escherichia coli have different metabolic origins
    Lucia Forzi
    Max Planck Institut fur terrestrische Mikrobiologie, Karl von Frisch Str, Marburg, Germany
    FEBS Lett 581:3317-21. 2007
    ..To determine their metabolic origin, [NiFe]-hydrogenase-2 was isolated from Escherichia coli grown in the presence of L-[ureido-(13)C]citrulline, purified and analyzed ..
  94. ncbi The [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough is a bacterial lipoprotein lacking a typical lipoprotein signal peptide
    Filipa M A Valente
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, 1781 901 Oeiras, Portugal
    FEBS Lett 581:3341-4. 2007
    Desulfovibrio vulgaris Hildenborough has a membrane-bound [NiFeSe] hydrogenase whose mode of membrane association was unknown since it is constituted by two hydrophilic subunits...
  95. ncbi Structural and biological analysis of the metal sites of Escherichia coli hydrogenase accessory protein HypB
    Alistair V Dias
    Department of Chemistry, University of Toronto, Toronto, Ontario, Canada
    Biochemistry 47:11981-91. 2008
    The [NiFe]-hydrogenase protein produced by many types of bacteria contains a dinuclear metal center that is required for enzymatic activity...
  96. ncbi Electrochemical kinetic investigations of the reactions of [FeFe]-hydrogenases with carbon monoxide and oxygen: comparing the importance of gas tunnels and active-site electronic/redox effects
    Gabrielle Goldet
    Inorganic Chemistry Laboratory, Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QR, United Kingdom
    J Am Chem Soc 131:14979-89. 2009
    ....
  97. ncbi Metal binding activity of the Escherichia coli hydrogenase maturation factor HypB
    Michael R Leach
    Department of Chemistry, University of Toronto, Toronto, Ontario, Canada M5S 3H6
    Biochemistry 44:12229-38. 2005
    The formation of the [NiFe] metallocenter of Escherichia coli hydrogenase 3 requires the participation of proteins encoded by the hydrogenase pleiotropy operon hypABCDEF...
  98. pmc O2 reactions at the six-iron active site (H-cluster) in [FeFe]-hydrogenase
    Camilla Lambertz
    Lehrstuhl für Biochemie der Pflanzen, AG Photobiotechnologie, Ruhr Universitat Bochum, 44780 Bochum, Germany
    J Biol Chem 286:40614-23. 2011
    ..This mechanism suggests that blocking of ROS diffusion paths and/or altering the redox potential of the [4Fe4S] cubane by genetic engineering may yield improved O(2) tolerance in [FeFe]-hydrogenase.
  99. pmc Metabolic deficiences revealed in the biotechnologically important model bacterium Escherichia coli BL21(DE3)
    Constanze Pinske
    Institute for Microbiology, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    PLoS ONE 6:e22830. 2011
    ..hydrogen evolution was due solely to reduced activity of the formate dehydrogenase (FDH-H), not the hydrogenase component...
  100. ncbi Development of a cell-free system reveals an oxygen-labile step in the maturation of [NiFe]-hydrogenase 2 of Escherichia coli
    Basem Soboh
    Institute for Mikrobiologie, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    FEBS Lett 584:4109-14. 2010
    ..of hydrogenases 1, 2 and 3 we could reconstitute in vitro under strictly anaerobic conditions 10-15% of the hydrogenase activity present in wild type Escherichia coli extracts...
  101. ncbi Biodiversity of uptake hydrogenase systems from legume endosymbiotic bacteria
    B Brito
    Laboratorio de Microbiologia, E T S Ingenieros Agronomos, Universidad Politecnica de Madrid, Ciudad Universitaria s n, 28040 Madrid, Spain
    Biochem Soc Trans 33:33-5. 2005
    ..Characterization of hydrogenase gene clusters in strains belonging to Rhizobium, Bradyrhizobium and Azorhizobium reveals a similar overall ..

Research Grants36

  1. pH Regulation in Bacillus subtilis (AREA R15)
    JOAN SLONCZEWSKI; Fiscal Year: 2009
    ..pH regulation of hydrogenase enzymes has implications for the biological production of hydrogen fuel...
  2. Role of FHL-mediated formate metabolism in Mycobacterium tuberculosis persistence
    Thomas C Zahrt; Fiscal Year: 2013
    ..tuberculosis during periods of NRP when the bacterium is otherwise recalcitrant to currently available antibiotics. ..
  3. BIOLOGICAL METAL CLUSTERS--BIOPHYSICAL AND MODEL STUDIES
    Michael Maroney; Fiscal Year: 1999
    ..g. superoxide dismutase, Cu, Zn; nitrogenase, Mo, Fe; cytochrome oxidase, heme a3, CuB; hydrogenase, acetyl coenzyme A synthase, carbon monoxide dehydrogenase, Ni, Fe)...
  4. Use of Molecular Hydrogen by Salmonella typhimurium
    Robert J Maier; Fiscal Year: 2010
    ..The affects of nickel chelators (i.e. nickel starvation conditions) on the expression of each individual hydrogenase will be assessed by studying the mutant strains...
  5. Development of a Novel Artificial Diiron Protein with N-hydroxylase Activity
    Marcos M Pires; Fiscal Year: 2010
    ..AurF is a recently discovered N-hydrogenase involved in the biosynthesis of the polyketide antibiotic aureothin...
  6. Evolution of Biological Hydrogen Production
    BUZ MICHAEL BARSTOW; Fiscal Year: 2012
    ..of O{2}-tolerant Hydrogenases using Genetic Screens Aim 2: Construction, Characterization and Evolution of a Library of Novel FeFe-hydrogenases Aim 3: Characterization and Evolution of Hydrogenase Accessory Proteins
  7. CHIROPTICAL SPECTROSCOPY OF METALLOPROTEINS
    Philip Stephens; Fiscal Year: 1990
    ..Specific metalloproteins to be studied include the iron-sulfur (Fe-S) proteins: Desulfovibrio vulgaris hydrogenase, Klebsiella pneumoniae pyruvate-flavodoxin oxidoreductase, and Micrococcus aerogenes ferredoxin and the nickel-..
  8. Synchrotron NRVS and Campus Spectroscopy of Multinuclear Fe Proteins: N2ase, H2as
    Stephen P Cramer; Fiscal Year: 2013
    ..of three classes of Fe-based metalloenzymes with 'unusual'active sites -- nitrogenase (N2ase), hydrogenase (H2ase), and methane monooxygenase (MMO)...
  9. BIOLOGICAL METAL CLUSTERS--BIOPHYSICAL & MODEL STUDIES
    Michael Maroney; Fiscal Year: 1991
    ..Synthetic models for the hydrogenase and CO dehydrogenase active sites and proposed intermediates are defined, and the study of their spectroscopic ..
  10. Preventing Protein Aggregation by Controlling Unfolded State Dynamics
    Lisa J Lapidus; Fiscal Year: 2013
    ..Alzheimer's A? and ?-synuclein will be measured in equilibrium, and hydrogenase maturation protein and various mammalian prion proteins will be measured in a novel microfluidic mixer that ..
  11. PHYSIOLOGY OF BACTERIAL NICKEL METABOLISM
    Robert Maier; Fiscal Year: 1991
    ..the routes of nickel binding, internalization, metabolism, and incorporation into a bacterial enzyme (hydrogenase) in the bacterium Bradyrhizobium japonicum. The specific aims are to: 1...
  12. BIOLOGICALLY RELATED IRON SULFUR CHEMISTRY
    Richard Holm; Fiscal Year: 2000
    ..and cluster enlargement, as a route to uncharacterized biological clusters such as the Hclusters of hydrogenase; (11) utilization of high-nuclearity clusters as a means to the preparation and characterization of V-Fe-S and ..
  13. ASSESSING ROLES OF NICKEL HYDRIDES IN HYDROGENASES
    Marcetta Darensbourg; Fiscal Year: 1993
    ..of heterolytic dihydrogen cleavage/activation mechanisms as a model of the expected Ni(mu-SR)Fe sites in hydrogenase. An examination of hydride and small molecule binding by recently prepared is also proposed. (4...
  14. MOSSBAUER AND EPR STUDIES OF HYDROGENASE
    Boi Hanh Huynh; Fiscal Year: 1991
    b>Hydrogenase are used by many micro-organisms, including algae, to metabolize H2. During the metabolic energy-yielding process, H2 is either oxidized or evolved as the product...
  15. ACETOGENIC AUTOTROPHY AND METABOLISM OF NICKEL
    HAROLD DRAKE; Fiscal Year: 1990
    ..function of the nickel enzyme carbon monoxide dehydrogenase will be further resolved and acetogenic hydrogenase will be purified and characterized...
  16. SOFT X-RAY AND EXAFS SPECTROSCOPY OF METALLOPROTEINS
    STEPHEN CRAMER; Fiscal Year: 2002
    ..Three Ni- contain enzymes that catalyze environmentally important processes, hydrogenase, methyl-Com reductase, and CO dehydrogenase have been chosen for investigation...
  17. ANALOGS FOR IRON CENTER IN HYDROGENASE ENZYMES
    Stephen Koch; Fiscal Year: 2003
    ..The active site of Ni-Fe hydrogenase contains an unexpected and unprecedented [Fe(CN)2(CO)] center...
  18. Nickel Metabolism in Helicobacter pylori
    Robert Maier; Fiscal Year: 2006
    ..Two nickel-containing enzymes, urease and hydrogenase, are important for the bacterium's mucosal-colonizing abilities...
  19. Repair of methionine residues in H. pylori catalase
    Robert Maier; Fiscal Year: 2008
    ..It is proposed to understand what factors influence the need for this repair in a key protein. [unreadable] [unreadable] [unreadable] [unreadable]..
  20. Virulence Determinants in Helicobacter hepaticus
    Robert Maier; Fiscal Year: 2004
    ..in 2003, and some suspected virulence factors (superoxide dismutase, alkyl hydroperoxide reductase, and hydrogenase) have been identified by enzyme assays on H. hepaticus extracts (in the Principal Investigator's lab)...
  21. Oxidative Stress and Survival of Helicobacter pylori
    Robert Maier; Fiscal Year: 2005
    ....
  22. The Role of MgATP in Nitrogenase Catalysis
    John Peters; Fiscal Year: 2007
    ..abstract_text> ..
  23. CRYSTALLOGRAPHIC STUDIES OF ELECTRON TRANSFER PROTEINS
    Douglas Rees; Fiscal Year: 2002
    ..4. the correspondence between crystallographically defined metallocluster structures and spectroscopically assigned oxidation states in the Fe-protein and MoFe-protein. ..
  24. Novel Responses to Oxygen by Anaerobic Microorganisms
    Michael Adams; Fiscal Year: 2008
    ..abstract_text> ..
  25. X-ray studies of metalloenzyme regulation and catalysis
    CATHERINE DRENNAN; Fiscal Year: 2008
    ..We will complement structural data with biochemical characterization and single crystal spectroscopic techniques. ..
  26. SUMMER RESEARCH IN MACROMOLECULAR INTERACTIONS
    CATHERINE DRENNAN; Fiscal Year: 2004
    ..The proposed training project complements institutional goals of expanding career choices for students majoring in physical and quantitative sciences, and increasing minority participation in undergraduate research programs. ..
  27. Metabolism of Carcinogens and Drugs by Human P450s
    F Guengerich; Fiscal Year: 2005
    ..Successful completion of these collective studies should provide better understanding of the ways in which human P450s catalyze oxidation of carcinogens and drugs and the significance of variations in these enzymes. ..
  28. Translational Development of Novel MMP Inhibitors
    Seth M Cohen; Fiscal Year: 2010
    ..3. To evaluate novel MPIs using isolated working rat heart preparations. 4. To examine the effects of MPIs on in vivo ischemia reperfusion injury. ..
  29. IMMUNODOMINANT TARGETS OF CMI TO CRYPTOSPORIDIUM
    Jan Mead; Fiscal Year: 2009
    ..This will be accomplished by immunizing mice with a DNA construct of these antigens, assessing their ability to elicit immune responses, and challenging mice with C. parvum to determine the degree of protection achieved. ..
  30. A Bioinorganic Approach to Anthrax Lethal Factor Inhibitors
    Seth Cohen; Fiscal Year: 2008
    ..Molecules will be designed and synthesized to attack a key toxin produced by the anthrax bacteria. In this way, innovative therapeutics for treating anthrax outbreaks will be developed. [unreadable] [unreadable] [unreadable]..
  31. Experimental R&D for Rapid Sequencing Nanotechnology
    James Lee; Fiscal Year: 2007
    ..This (R21/R33) R&D project proposal is in response to NIH RFA-HG-04-003 program announcement for Revolutionary Genome Sequencing Technologies. [unreadable] [unreadable]..
  32. Computational R&D for Rapid Sequencing Nanotechnology
    James Lee; Fiscal Year: 2007
    ..This (R21/R33) project proposal is in response to NIH RFA-HG-04-003 program announcement for Revolutionary Genome Sequencing Technologies. [unreadable] [unreadable]..
  33. Generation and Repair of an Unusual UV Photoproduct
    Joan Broderick; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  34. Engineering de novo oxidoreductases
    Anne Jones; Fiscal Year: 2006
    ..Motifs believed to play a role in hydrogenase mechanism will be individually studied in the maquette model via structure determination and spectroscopic ..