malate dehydrogenase

Summary

Summary: An enzyme that catalyzes the conversion of (S)-malate and NAD+ to oxaloacetate and NADH. EC 1.1.1.37.

Top Publications

  1. ncbi Regulation of malate dehydrogenase activity by glutamate, citrate, alpha-ketoglutarate, and multienzyme interaction
    L A Fahien
    Department of Pharmacology, University of Wisconsin Medical School, Madison 53706
    J Biol Chem 263:10687-97. 1988
  2. pmc Mitochondrial malate dehydrogenase lowers leaf respiration and alters photorespiration and plant growth in Arabidopsis
    Tiago Tomaz
    Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia, Australia
    Plant Physiol 154:1143-57. 2010
  3. doi A nucleocytoplasmic malate dehydrogenase regulates p53 transcriptional activity in response to metabolic stress
    S M Lee
    Department of Biomedical Sciences and Biochemistry and Molecular Biology, National Research Laboratory for Metabolic Checkpoint, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea
    Cell Death Differ 16:738-48. 2009
  4. ncbi Degradation of the gluconeogenic enzymes fructose-1,6-bisphosphatase and malate dehydrogenase is mediated by distinct proteolytic pathways and signaling events
    Guo Chiuan Hung
    Department of Cellular and Molecular Physiology, Penn State University College of Medicine, Hershey, Pennsylvania 17033, USA
    J Biol Chem 279:49138-50. 2004
  5. ncbi Endocrine active compounds affect thyrotropin and thyroid hormone levels in serum as well as endpoints of thyroid hormone action in liver, heart and kidney
    Cornelia Schmutzler
    Institut für Experimentelle Endokrinologie und Endokrinologisches Forschungszentrum EnForCé, Charite, Universitatsmedizin Berlin, Campus Mitte, Schumannstrasse 20 21, D 10117 Berlin, Germany
    Toxicology 205:95-102. 2004
  6. ncbi A role for ATP-citrate lyase, malic enzyme, and pyruvate/citrate cycling in glucose-induced insulin secretion
    Claudiane Guay
    Molecular Nutrition Unit, Montreal Diabetes Research Center, Centre de recherche du Centre Hospitalier de l Universite de Montreal, University of Montreal, Montreal, Quebec H1W 4A4, Canada
    J Biol Chem 282:35657-65. 2007
  7. pmc The membrane of peroxisomes in Saccharomyces cerevisiae is impermeable to NAD(H) and acetyl-CoA under in vivo conditions
    C W van Roermund
    Department of Clinical Biochemistry, University of Amsterdam, The Netherlands
    EMBO J 14:3480-6. 1995
  8. pmc Enzyme:nanoparticle bioconjugates with two sequential enzymes: stoichiometry and activity of malate dehydrogenase and citrate synthase on Au nanoparticles
    Jacqueline D Keighron
    Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, United States
    Langmuir 26:18992-9000. 2010
  9. ncbi Transcriptional regulation of NADP-dependent malate dehydrogenase: comparative genetics and identification of DNA-binding proteins
    Steffen Hameister
    Pflanzenphysiologie, Fachbereich Biologie chemie, Universitat Osnabruck, Osnabruck, Germany
    J Mol Evol 65:437-55. 2007
  10. pmc Peroxisomal malate dehydrogenase is not essential for photorespiration in Arabidopsis but its absence causes an increase in the stoichiometry of photorespiratory CO2 release
    Asaph B Cousins
    School of Biological Sciences, Washington State University, Pullman, WA 99164 4236, USA
    Plant Physiol 148:786-95. 2008

Detail Information

Publications303 found, 100 shown here

  1. ncbi Regulation of malate dehydrogenase activity by glutamate, citrate, alpha-ketoglutarate, and multienzyme interaction
    L A Fahien
    Department of Pharmacology, University of Wisconsin Medical School, Madison 53706
    J Biol Chem 263:10687-97. 1988
    ..aminotransferase can associate with the alpha-ketoglutarate dehydrogenase complex and that mitochondrial malate dehydrogenase can associate with this binary complex to form a ternary complex...
  2. pmc Mitochondrial malate dehydrogenase lowers leaf respiration and alters photorespiration and plant growth in Arabidopsis
    Tiago Tomaz
    Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia, Australia
    Plant Physiol 154:1143-57. 2010
    b>Malate dehydrogenase (MDH) catalyzes a reversible NAD(+)-dependent-dehydrogenase reaction involved in central metabolism and redox homeostasis between organelle compartments...
  3. doi A nucleocytoplasmic malate dehydrogenase regulates p53 transcriptional activity in response to metabolic stress
    S M Lee
    Department of Biomedical Sciences and Biochemistry and Molecular Biology, National Research Laboratory for Metabolic Checkpoint, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea
    Cell Death Differ 16:738-48. 2009
    ..Here, we discover that nucleocytoplasmic malate dehydrogenase-1 (MDH1) physically associates with p53...
  4. ncbi Degradation of the gluconeogenic enzymes fructose-1,6-bisphosphatase and malate dehydrogenase is mediated by distinct proteolytic pathways and signaling events
    Guo Chiuan Hung
    Department of Cellular and Molecular Physiology, Penn State University College of Medicine, Hershey, Pennsylvania 17033, USA
    J Biol Chem 279:49138-50. 2004
    ..Another gluconeogenic enzyme malate dehydrogenase (MDH2) showed the same degradation characteristics as FBPase in that the short term starvation of cells led ..
  5. ncbi Endocrine active compounds affect thyrotropin and thyroid hormone levels in serum as well as endpoints of thyroid hormone action in liver, heart and kidney
    Cornelia Schmutzler
    Institut für Experimentelle Endokrinologie und Endokrinologisches Forschungszentrum EnForCé, Charite, Universitatsmedizin Berlin, Campus Mitte, Schumannstrasse 20 21, D 10117 Berlin, Germany
    Toxicology 205:95-102. 2004
    ....
  6. ncbi A role for ATP-citrate lyase, malic enzyme, and pyruvate/citrate cycling in glucose-induced insulin secretion
    Claudiane Guay
    Molecular Nutrition Unit, Montreal Diabetes Research Center, Centre de recherche du Centre Hospitalier de l Universite de Montreal, University of Montreal, Montreal, Quebec H1W 4A4, Canada
    J Biol Chem 282:35657-65. 2007
    ..Taken together, the results underscore the importance of pyruvate/citrate cycling in pancreatic beta-cell metabolic signaling and the regulation of GIIS...
  7. pmc The membrane of peroxisomes in Saccharomyces cerevisiae is impermeable to NAD(H) and acetyl-CoA under in vivo conditions
    C W van Roermund
    Department of Clinical Biochemistry, University of Amsterdam, The Netherlands
    EMBO J 14:3480-6. 1995
    ..Disruption of the peroxisomal malate dehydrogenase 3 gene (MDH3) resulted in impaired beta-oxidation capacity as measured in intact cells, whereas beta-..
  8. pmc Enzyme:nanoparticle bioconjugates with two sequential enzymes: stoichiometry and activity of malate dehydrogenase and citrate synthase on Au nanoparticles
    Jacqueline D Keighron
    Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, United States
    Langmuir 26:18992-9000. 2010
    We report the synthesis and characterization of bioconjugates in which the enzymes malate dehydrogenase (MDH) and/or citrate synthase (CS) were adsorbed to 30 nm diameter Au nanoparticles...
  9. ncbi Transcriptional regulation of NADP-dependent malate dehydrogenase: comparative genetics and identification of DNA-binding proteins
    Steffen Hameister
    Pflanzenphysiologie, Fachbereich Biologie chemie, Universitat Osnabruck, Osnabruck, Germany
    J Mol Evol 65:437-55. 2007
    The transcriptional regulation of NADP-malate dehydrogenase (NADP-MDH) was analyzed in Arabidopsis ecotypes and other Brassicaceae...
  10. pmc Peroxisomal malate dehydrogenase is not essential for photorespiration in Arabidopsis but its absence causes an increase in the stoichiometry of photorespiratory CO2 release
    Asaph B Cousins
    School of Biological Sciences, Washington State University, Pullman, WA 99164 4236, USA
    Plant Physiol 148:786-95. 2008
    ..reductase (HPR) in the peroxisomes is thought to be facilitated by the production of NADH by peroxisomal malate dehydrogenase (PMDH)...
  11. ncbi Evolution of Cryptosporidium parvum lactate dehydrogenase from malate dehydrogenase by a very recent event of gene duplication
    Dominique Madern
    Laboratoire de Biophysique Moleculaire, Institut de Biologie Structurale CEA CNRS UJF, Grenoble, France
    Mol Biol Evol 21:489-97. 2004
    We have expressed the L-lactate dehydrogenase (LDH) and L-malate dehydrogenase (malDH) genes from the apicomplexan Cryptosporidium parvum (CpLDH1 and CpMalDH1) as maltose-binding protein (MBP) fusion proteins in Escherichia coli...
  12. pmc Disruption of mitochondrial malate-aspartate shuttle activity in mouse blastocysts impairs viability and fetal growth
    Megan Mitchell
    Research Centre for Reproductive Health, University of Adelaide, Adelaide, South Australia 5005, Australia
    Biol Reprod 80:295-301. 2009
    ....
  13. ncbi Malate dehydrogenase: distribution, function and properties
    R A Musrati
    Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic
    Gen Physiol Biophys 17:193-210. 1998
    b>Malate dehydrogenase (MDH) (EC 1.1.1.37) catalyzes the conversion of oxaloacetate and malate. This reaction is important in cellular metabolism, and it is coupled with easily detectable cofactor oxidation/reduction...
  14. ncbi Expression and function of a mislocalized form of peroxisomal malate dehydrogenase (MDH3) in yeast
    L McAlister-Henn
    Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284, USA
    J Biol Chem 270:21220-5. 1995
    The malate dehydrogenase isozyme MDH3 of Saccharomyces cerevisiae was found to be localized to peroxisomes by cellular fractionation and density gradient centrifugation...
  15. doi A redox-active isopropylmalate dehydrogenase functions in the biosynthesis of glucosinolates and leucine in Arabidopsis
    Yan He
    Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610, USA
    Plant J 60:679-90. 2009
    ..It provides evidence for the hypothesis that the two pathways share a common origin, and suggests a role for redox regulation of glucosinolate and leucine synthesis in plants...
  16. pmc Overexpression of malate dehydrogenase in transgenic alfalfa enhances organic acid synthesis and confers tolerance to aluminum
    M Tesfaye
    Department of Plant Pathology, University of Minnesota, St. Paul, Minnesota 55108, USA
    Plant Physiol 127:1836-44. 2001
    ..Al tolerance in alfalfa (Medicago sativa), we produced transgenic plants using nodule-enhanced forms of malate dehydrogenase and phosphoenolpyruvate carboxylase cDNAs under the control of the constitutive cauliflower mosaic virus ..
  17. pmc Malic acid production by Saccharomyces cerevisiae: engineering of pyruvate carboxylation, oxaloacetate reduction, and malate export
    Rintze M Zelle
    Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
    Appl Environ Microbiol 74:2766-77. 2008
    ..carboxylase encoded by PYC2, (ii) high-level expression of an allele of the MDH3 gene, of which the encoded malate dehydrogenase was retargeted to the cytosol by deletion of the C-terminal peroxisomal targeting sequence, and (iii) ..
  18. ncbi Viability effects and not meoitic drive cause dramatic departures from Mendelian inheritance for malic enzyme in hybrids of Tigriopus californicus populations
    C S Willett
    Department of Biology, University of North Carolina, Chapel Hill, Chapel Hill, NC, USA
    J Evol Biol 20:1196-205. 2007
    ..These results imply that selection against deleterious gene combinations and not aberrant segregation (i.e. meiotic drive) is likely to lead to dramatic departures from Mendelian inheritance observed in these crosses...
  19. ncbi Solvent interactions of halophilic malate dehydrogenase
    Christine Ebel
    Laboratoire de Biophysique Moleculaire, Institut de Biologie Structurale, UMR 5075 CEA CNRS UJF, 41 rue Jules Horowitz, 38027 Grenoble, France
    Biochemistry 41:13234-44. 2002
    b>Malate dehydrogenase from the extreme halophilic Haloarcula marismortui (Hm MalDH) is an acidic protein that is unstable below molar salt concentrations...
  20. ncbi Malolactic fermentation by Streptococcus mutans
    Jiangyun Sheng
    Department of Microbiology and Immunology and Center for Oral Biology, University of Rochester Medical Center, Rochester, NY 14642, USA
    FEMS Microbiol Lett 272:196-201. 2007
    ..The net conclusion is that malate has the potential to be effective for alkalinization of dental plaque, although the fermentation is sensitive to fluoride and triclosan, which are commonly added to oral care products...
  21. ncbi Fatty acid beta-oxidation in germinating Arabidopsis seeds is supported by peroxisomal hydroxypyruvate reductase when malate dehydrogenase is absent
    Itsara Pracharoenwattana
    Australian Research Council Centre of Excellence in Plant Energy Biology and Centre of Excellence for Plant Metabolomics, Molecular and Chemical Sciences Building M316, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia
    Plant Mol Biol 72:101-9. 2010
    Peroxisomal malate dehydrogenase (PMDH) oxidises NADH produced by fatty acid beta-oxidation during seed germination and seedling growth...
  22. ncbi Mutation at a single acidic amino acid enhances the halophilic behaviour of malate dehydrogenase from Haloarcula marismortui in physiological salts
    D Madern
    Institut de Biologie Structurale, Grenoble, France
    Eur J Biochem 230:1088-95. 1995
    ..The role of acidic residues in halophilic adaptation was investigated by site-directed mutagenesis of malate dehydrogenase (MalDH) from Haloarcula marismortui...
  23. pmc Quantifying interactions within the NADP(H) enzyme network in Drosophila melanogaster
    Thomas J S Merritt
    Department of Ecology and Evolution, Stony Brook University, Stony Brook, NY 11794, USA
    Genetics 182:565-74. 2009
    ..melanogaster using naturally occurring alleles. The interactions between all three enzymes indicate functional interdependence and underscore the importance of examining enzymes as components of a network...
  24. pmc Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase
    Yifeng Yin
    QB3 Institute, 572 Stanley Hall, University of California, Berkeley, CA 94720 3220, USA
    Proc Natl Acad Sci U S A 104:17353-7. 2007
    Five positions in the Escherichia coli malate dehydrogenase (eMDH) sequence, which distinguish MDH from lactate dehydrogenase (LDH) activity, were identified through a combination of Venn diagrams constructed from whole genomic data and ..
  25. ncbi Role of cytosolic malate dehydrogenase in oocyte maturation and embryo development
    Se Jin Yoon
    CHA Research Institute, Fertility Center, CHA General Hospital, Seoul, Korea
    Fertil Steril 86:1129-36. 2006
    To elucidate the function of cytosolic malate dehydrogenase (Mor2) in oocyte maturation and embryo development using RNA interference (RNAi).
  26. doi Malate dehydrogenase is negatively regulated by miR-1 in Trichomonas vaginalis
    Wei Chen Lin
    Molecular Regulation and Bioinformatics Laboratory, Chang Gung University, Taoyuan, Taiwan
    Parasitol Res 105:1683-9. 2009
    ..We observed that the T. vaginalis malate dehydrogenase (Tv_MDH) gene was upregulated 20-fold in the amoeboid stage, but the protein level was reduced by 4.5-fold...
  27. ncbi Physical and genetic interactions of cytosolic malate dehydrogenase with other gluconeogenic enzymes
    Natalie Gibson
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas 78229 3900, USA
    J Biol Chem 278:25628-36. 2003
    A truncated form (deltanMDH2) of yeast cytosolic malate dehydrogenase (MDH2) lacking 12 residues on the amino terminus was found to be inadequate for gluconeogenic function in vivo because the mutant enzyme fails to restore growth of a ..
  28. ncbi The visualization by affinity electrophoresis of a specific association between the consecutive citric acid cycle enzymes fumarase and malate dehydrogenase
    S Beeckmans
    Laboratorium voor Chemie der Proteinen, Vrije Universiteit Brussel, Belgium
    Eur J Biochem 183:449-54. 1989
    ..technique of affinity electrophoresis for the study of interactions between the cycle enzymes fumarase and malate dehydrogenase. This approach offers several advantages over our previously described affinity chromatographic technique [..
  29. pmc Acetylation of malate dehydrogenase 1 promotes adipogenic differentiation via activating its enzymatic activity
    Eun Young Kim
    Department of Biological Sciences, KAIST, Daejeon 305 701, Republic of Korea
    J Lipid Res 53:1864-76. 2012
    ..Of these identified proteins, we focused on malate dehydrogenase 1 (MDH1). The acetylation level of MDH1 was increased up to 6-fold at the late stage of adipogenesis...
  30. ncbi Structural analyses of a malate dehydrogenase with a variable active site
    J K Bell
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis 55455, USA
    J Biol Chem 276:31156-62. 2001
    b>Malate dehydrogenase specifically oxidizes malate to oxaloacetate...
  31. pmc Non-ideality by sedimentation velocity of halophilic malate dehydrogenase in complex solvents
    A Solovyova
    , Institut de Biologie Structurale J. P. Ebel, F-38027 Grenoble, France
    Biophys J 81:1868-80. 2001
    ..by direct boundary modeling, and applied it to data from sedimentation velocity experiments on halophilic malate dehydrogenase in complex aqueous solvents containing sodium chloride and 2-methyl-2,4-pentanediol, including conditions ..
  32. doi Isolation and characterization of an apple cytosolic malate dehydrogenase gene reveal its function in malate synthesis
    Yu Xin Yao
    State Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai an, Shandong 271018, China
    J Plant Physiol 168:474-80. 2011
    Cytosolic NAD-dependent malate dehydrogenase (cyMDH) is an enzyme crucial for malate synthesis in the cytosol. The apple MdcyMDH gene (GenBank Accession No...
  33. pmc Structural studies of the pigeon cytosolic NADP(+)-dependent malic enzyme
    Zhiru Yang
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Protein Sci 11:332-41. 2002
    ..Our structural studies also revealed differences in the organization of the tetramer between the pigeon and the human enzymes, although the pigeon enzyme still obeys 222 symmetry...
  34. ncbi Role of threonine 190 in modulating the catalytic function of malate dehydrogenase from a thermophile Thermus flavus
    M Nishiyama
    Department of Agricultural Chemistry, University of Tokyo, Japan
    J Biol Chem 266:14294-9. 1991
    Random mutagenesis of malate dehydrogenase from a thermophilic bacterium, Thermus flavus AT-62, had revealed that a Thr190----Ile replacement near the essential catalytic residue His187 caused marked modulation of the catalytic properties...
  35. ncbi Differences between mouse and rat pancreatic islets: succinate responsiveness, malic enzyme, and anaplerosis
    Michael J MacDonald
    University of Wisconsin Childrens Diabetes Center, Madison, Wisconsin 53706, USA gt
    Am J Physiol Endocrinol Metab 283:E302-10. 2002
    ....
  36. ncbi The folding of dimeric cytoplasmic malate dehydrogenase. Equilibrium and kinetic studies
    Suparna C Sanyal
    Department of Biophysics, Molecular Biology and Genetics, University of Calcutta, Kolkata, India
    Eur J Biochem 269:3856-66. 2002
    Porcine heart cytoplasmic malate dehydrogenase (s-MDH) is a dimeric protein (2 x 35 kDa)...
  37. ncbi The molecular basis for a cytosolic malic enzyme null mutation. Malic enzyme mRNA from MOD-1 null mice contains an internal in-frame duplication that extends the coding sequence by 522 nucleotides
    M L Brown
    Department of Molecular Pharmacology, Atran Laboratories, Albert Einstein College of Medicine, Bronx, New York 10461
    J Biol Chem 263:4494-9. 1988
    ..The MOD-1 null mutation might originate from an unequal crossover between homologous regions of two different introns in the malic enzyme gene, thereby causing the duplication of one or more exons...
  38. pmc Direct evidence that genetic variation in glycerol-3-phosphate and malate dehydrogenase genes (Gpdh and Mdh1) affects adult ethanol tolerance in Drosophila melanogaster
    Walter F Eanes
    Department of Ecology and Evolution, Stony Brook University, Stony Brook, New York 11794, USA
    Genetics 181:607-14. 2009
    ..Here we evaluate the effects of glycerol-3-phosphate dehydrogenase (Gpdh) and cytosolic malate dehydrogenase (Mdh1) genotype activity on adult tolerance to ethanol...
  39. ncbi Localization, structure and polymorphism of two paralogous Xenopus laevis mitochondrial malate dehydrogenase genes
    Tereza Tlapakova
    Department of Animal Physiology and Developmental Biology, Faculty of Science, Charles University in Prague, Vinicna 7, Prague 2, 128 43, Czech Republic
    Chromosome Res 13:699-706. 2005
    Two paralogous mitochondrial malate dehydrogenase 2 (Mdh2) genes of Xenopus laevis have been cloned and sequenced, revealing 95% identity...
  40. doi The functions of an apple cytosolic malate dehydrogenase gene in growth and tolerance to cold and salt stresses
    Yu Xin Yao
    State Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai an, Shandong, China
    Plant Physiol Biochem 49:257-64. 2011
    It is well-known that cytosolic NAD-dependent malate dehydrogenase (cyMDH; l-malate:NAD-oxidoreductase; EC 1.1.1.37) is an enzyme crucial for malic acid synthesis in the cytosol. Nothing is known about cyMDH in growth and stress tolerance...
  41. ncbi Role of CCAAT/enhancer-binding protein, histone acetylation, and coactivator recruitment in the regulation of malic enzyme transcription by thyroid hormone
    Liya Yin
    Department of Biochemistry and Molecular Pharmacology, School of Medicine, P O Box 9142, West Virginia University, Morgantown, 26506 9142, USA
    Mol Cell Endocrinol 245:43-52. 2005
    ....
  42. ncbi Dictyostelium differentiation-inducing factor-1 binds to mitochondrial malate dehydrogenase and inhibits its activity
    Tomoko Matsuda
    Department of Clinical Pharmacology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan
    J Pharmacol Sci 112:320-6. 2010
    ..study, DIF-1-tethered resins were synthesized to explore the target molecules of DIFs, and mitochondrial malate dehydrogenase (mMDH) was identified as one of the target molecules...
  43. ncbi Electrostatic interactions across the dimer-dimer interface contribute to the pH-dependent stability of a tetrameric malate dehydrogenase
    Alexandra Bjørk
    Department of Biology, University of Oslo, Box 1031 Blindern, N 0316 Oslo, Norway
    FEBS Lett 553:423-6. 2003
    b>Malate dehydrogenase (MDH) from the moderately thermophilic bacterium Chloroflexus aurantiacus (CaMDH) is a tetrameric enzyme, while MDHs from mesophilic bacteria usually are dimers...
  44. pmc Alternative splicing regulates targeting of malate dehydrogenase in Yarrowia lipolytica
    Philomène Kabran
    INRA, UMR 1319 Micalis, F 78352 Jouy en Josas, France
    DNA Res 19:231-44. 2012
    ..The splicing pattern was found to govern the cellular localization of malate dehydrogenase, an enzyme of the central carbon metabolism...
  45. pmc Nonclassical regulation of transcription: interchromosomal interactions at the malic enzyme locus of Drosophila melanogaster
    Thomas E Lum
    Department of Chemistry and Biochemistry, Laurentian University, Sudbury, Ontario P3E 2C6, Canada
    Genetics 189:837-49. 2011
    ..In sum, these results emphasize the complexity of gene regulation and the need to understand both small- and large-scale interactions as more complete models of the role of trans-interactions in gene regulation are developed...
  46. ncbi Isolation and characterization of the yeast gene encoding the MDH3 isozyme of malate dehydrogenase
    J S Steffan
    Department of Biological Chemistry, College of Medicine, University of California, Irvine 92717
    J Biol Chem 267:24708-15. 1992
    ..as a carbon source, and under these conditions, MDH3 contributed approximately 10% of the total cellular malate dehydrogenase activity...
  47. ncbi Enzymatic urea adaptation: lactate and malate dehydrogenase in elasmobranchs
    G Lagana
    Department of Organic and Biological Chemistry, University of Messina Salita Sperone, Messina, Italy
    Physiol Res 55:675-88. 2006
    Lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) electrophoretic tissue patterns of two different orders of Elasmobranchii: Carchariniformes (Galeus melanostomus and Prionace glauca) and Squaliformes (Etmopterus spinax and ..
  48. ncbi Insertion of transposon Tn5tac1 in the Sinorhizobium meliloti malate dehydrogenase (mdh) gene results in conditional polar effects on downstream TCA cycle genes
    Sergiy I Dymov
    Department of Natural Resource Sciences, McGill University, QC, Canada
    Mol Plant Microbe Interact 17:1318-27. 2004
    To isolate Sinorhizobium meliloti mutants deficient in malate dehydrogenase (MDH) activity, random transposon Tn5tac1 insertion mutants were screened for conditional lethal phenotypes on complex medium...
  49. ncbi The Oligomeric states of Haloarcula marismortui malate dehydrogenase are modulated by solvent components as shown by crystallographic and biochemical studies
    Adriana Irimia
    Laboratoire de Biophysique Moleculaire, Institut de Biologie Structurale J P Ebel CEA CNRS UJF UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex 01, France
    J Mol Biol 326:859-73. 2003
    The three-dimensional crystal structure of the (R207S, R292S) mutant of malate dehydrogenase from Haloarcula marismortui was solved at 1...
  50. pmc Isolation and expression of the gene encoding yeast mitochondrial malate dehydrogenase
    L McAlister-Henn
    Department of Biological Chemistry, California College of Medicine, University of California, Irvine 92717
    J Bacteriol 169:5157-66. 1987
    The mitochondrial tricarboxylic acid cycle enzyme malate dehydrogenase was purified from Saccharomyces cerevisiae, and an antibody to the purified enzyme was obtained in rabbits...
  51. ncbi Molecular adaptation: the malate dehydrogenase from the extreme halophilic bacterium Salinibacter ruber behaves like a non-halophilic protein
    Dominique Madern
    Laboratoire de Biophysique Moleculaire, Institut de Biologie Structurale CEA CNRS UJF, UMR 5075, 41, rue Jules Horowitz, Grenoble 38042, France
    Biochimie 86:295-303. 2004
    b>Malate dehydrogenase from the extreme halophilic bacterium, Salinibacter ruber (Sr MalDH) was purified and characterised as a tetramer by sedimentation velocity measurements, showing the enzyme belongs to the LDH-like group of MalDHs...
  52. ncbi Gene sequence and primary structure of mitochondrial malate dehydrogenase from Saccharomyces cerevisiae
    L M Thompson
    Department of Biological Chemistry, California College of Medicine, University of California, Irvine 92717
    Biochemistry 27:8393-400. 1988
    The nucleotide sequence was determined for a 1.5-kilobase genomic fragment containing the mitochondrial malate dehydrogenase gene (MDH1) of Saccharomyces cerevisiae...
  53. ncbi Stabilization of a tetrameric malate dehydrogenase by introduction of a disulfide bridge at the dimer-dimer interface
    Alexandra Bjørk
    Department of Biology, University of Oslo, PO Box 1031 Blindern, N 0316 Oslo, Norway
    J Mol Biol 334:811-21. 2003
    b>Malate dehydrogenase (MDH) from the moderately thermophilic bacterium Chloroflexus aurantiacus (CaMDH) is a tetrameric enzyme, while MDHs from mesophilic organisms usually are dimers...
  54. pmc Isolation, nucleotide sequence analysis, and disruption of the MDH2 gene from Saccharomyces cerevisiae: evidence for three isozymes of yeast malate dehydrogenase
    K I Minard
    Department of Biological Chemistry, College of Medicine, University of California, Irvine 92717
    Mol Cell Biol 11:370-80. 1991
    The major nonmitochondrial isozyme of malate dehydrogenase (MDH2) in Saccharomyces cerevisiae cells grown with acetate as a carbon source was purified and shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to have a ..
  55. pmc Up-regulation of the mitochondrial malate dehydrogenase by oxidative stress is mediated by miR-743a
    Qingli Shi
    Department of Neurology and Neuroscience, Weill Cornell Medical College Burke Medical Research Institute, White Plains, New York, USA
    J Neurochem 118:440-8. 2011
    ..mdh2 encoded malate dehydrogenase (MDH) is elevated by an unknown mechanism in brains of patients that died with Alzheimer's disease...
  56. ncbi Determinants of the dual cofactor specificity and substrate cooperativity of the human mitochondrial NAD(P)+-dependent malic enzyme: functional roles of glutamine 362
    Ju Yi Hsieh
    Department of Life Sciences, National Chung Hsing University, Taichung 40227
    J Biol Chem 281:23237-45. 2006
    ..In this study, we provide clear evidence that the single mutation of Gln362 to Lys in human m-NAD-ME changes it to an NADP+-dependent enzyme, which is characteristic because it is non-allosteric, non-cooperative, and NADP+-specific...
  57. ncbi Crystal structure of the malic enzyme from Ascaris suum complexed with nicotinamide adenine dinucleotide at 2.3 A resolution
    David E Coleman
    Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, Texas 76107, USA
    Biochemistry 41:6928-38. 2002
    ..The structure is discussed in terms of the mechanism and particularly with respect to previously obtained kinetic and site-directed mutagenesis experiments...
  58. ncbi Dual functional roles of ATP in the human mitochondrial malic enzyme
    Wen Chi Hsu
    Faculty of Life Sciences, Institute of Biochemistry, Structure Biology Program, and Proteome Research Center, National Yang Ming University, Taipei 112, Taiwan
    Biochemistry 43:7382-90. 2004
    ..Mutation of a single residue Arg542 at the tetrameric interfacial exo site resulted in dimeric mutants. ATP thus has dual functional roles in the mitochondrial malic enzyme...
  59. ncbi Crystal structures of substrate complexes of malic enzyme and insights into the catalytic mechanism
    Xiao Tao
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Structure 11:1141-50. 2003
    ..Tyr112-Lys183 functions as the general acid-base pair to catalyze the tautomerization of the enolpyruvate product from decarboxylation to pyruvate...
  60. ncbi Functional roles of ATP-binding residues in the catalytic site of human mitochondrial NAD(P)+-dependent malic enzyme
    Hui Chih Hung
    Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan
    Biochemistry 44:12737-45. 2005
    ..It can be concluded that mutation of Glu314 to Ala eliminated the repulsive effects between Glu314 and malate, NAD+, or ATP, and thus the binding affinities of malate, NAD+, and ATP in the active site of the enzyme were enhanced...
  61. ncbi Crystal structure of human mitochondrial NAD(P)+-dependent malic enzyme: a new class of oxidative decarboxylases
    Y Xu
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Structure 7:R877-89. 1999
    ..Moreover, this isoform is unique among malic enzymes in that it is a cooperative enzyme, and its activity is controlled allosterically...
  62. ncbi Characterization of cytosolic malic enzyme in human tumor cells
    G Loeber
    Ernst Boehringer Institut, Vienna, Austria
    FEBS Lett 344:181-6. 1994
    ..Thus, NADP(+)-ME found in tumor cells does not constitute a tumor-specific isoform as suggested by other studies but is identical to the 'housekeeping protein' predominantly expressed in human liver and white adipose tissue...
  63. ncbi Human NAD(+)-dependent mitochondrial malic enzyme. cDNA cloning, primary structure, and expression in Escherichia coli
    G Loeber
    Ernst Boehringer Institut, Vienna, Austria
    J Biol Chem 266:3016-21. 1991
    ..Expression of the processed protein in E. coli yielded an enzyme with the same kinetic and allosteric properties as malic enzyme purified from human cells...
  64. ncbi Potent and competitive inhibition of malic enzymes by lanthanide ions
    Z Yang
    Department of Biological Sciences, Columbia University, New York, New York, 10027, USA
    Biochem Biophys Res Commun 274:440-4. 2000
    ....
  65. doi Proper positioning of the nicotinamide ring is crucial for the Ascaris suum malic enzyme reaction
    Deniz F Aktas
    Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, Oklahoma 73019, USA
    Biochemistry 47:2539-46. 2008
    ..Overall, data indicate that subtle changes in the orientation of the cofactor and substrate dramatically influence the reaction rate...
  66. ncbi Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface
    Alexandra Bjørk
    Department of Molecular Biosciences, University of Oslo, P O Box 1041, Blindern, N 0316 Oslo, Norway
    J Mol Biol 341:1215-26. 2004
    The stability of tetrameric malate dehydrogenase from the green phototrophic bacterium Chloroflexus aurantiacus (CaMDH) is at least in part determined by electrostatic interactions at the dimer-dimer interface...
  67. pmc Characterization of the functional role of allosteric site residue Asp102 in the regulatory mechanism of human mitochondrial NAD(P)+-dependent malate dehydrogenase (malic enzyme)
    Hui Chih Hung
    Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan, Republic of China
    Biochem J 392:39-45. 2005
    Human mitochondrial NAD(P)+-dependent malate dehydrogenase (decarboxylating) (malic enzyme) can be specifically and allosterically activated by fumarate...
  68. doi Malate dehydrogenase: a useful phylogenetic marker for the genus Aeromonas
    Maribel Farfán
    Departament de Microbiologia i Parasitologia Sanitaries, Facultat de Farmacia, Universitat de Barcelona, Avda Joan XXIII s n, 08028 Barcelona, Spain
    Syst Appl Microbiol 33:427-35. 2010
    ..In bacteria it has been proved that the malate dehydrogenase gene (mdh) can be used to determine the inter- and intraspecies divergence, and hence this gene constitutes ..
  69. ncbi An alpha-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum. Cloning and biochemical characterization of the enzyme
    Abhai K Tripathi
    National Center for Natural Product Research, Research Institute of Pharmacological Sciences, University of Mississippi, MS 38677, USA
    Eur J Biochem 271:3488-502. 2004
    b>Malate dehydrogenase (MDH) may be important in carbohydrate and energy metabolism in malarial parasites...
  70. ncbi Comparison of the precursor and mature forms of rat heart mitochondrial malate dehydrogenase
    P M Grant
    Biochemistry 26:128-34. 1987
    The complete amino acid sequence of mitochondrial malate dehydrogenase from rat heart has been determined by chemical methods...
  71. ncbi Arabidopsis peroxisomal malate dehydrogenase functions in beta-oxidation but not in the glyoxylate cycle
    Itsara Pracharoenwattana
    Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, The King s Buildings, Edinburgh EH9 3JH, UK
    Plant J 50:381-90. 2007
    The aim was to determine the function of peroxisomal NAD(+)-malate dehydrogenase (PMDH) in fatty acid beta-oxidation and the glyoxylate cycle in Arabidopsis...
  72. pmc Selective 13C labeling of nucleotides for large RNA NMR spectroscopy using an E. coli strain disabled in the TCA cycle
    Chandar S Thakur
    Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, 1115 Biomolecular Sciences Bldg 296, College Park, MD 20742 3360, USA
    J Biomol NMR 48:179-92. 2010
    ..Even under conditions of slow tumbling, all the expected carbon correlations were observed, which indicates this approach of using nucleotides obtained from DL323 E. coli will be applicable to high molecular weight RNA systems...
  73. ncbi Structure and function of malic enzymes, a new class of oxidative decarboxylases
    Gu Gang Chang
    Faculty of Life Sciences, Institute of Biochemistry, Proteome Research Center, National Yang Ming University, Taipei 112, Taiwan
    Biochemistry 42:12721-33. 2003
    ..In addition, a divalent metal ion (Mn(2+) or Mg(2+)) is essential in helping the catalysis. Binding of the metal ion also plays an important role in stabilizing the quaternary structural integrity of the enzyme...
  74. doi Engineering of the cofactor specificities and isoform-specific inhibition of malic enzyme
    Ju Yi Hsieh
    Department of Life Sciences and Institute of Bioinformatics, National Chung Hsing University, Taichung 40227, Taiwan
    J Biol Chem 284:4536-44. 2009
    ..In the triple mutant, Lys-362, Lys-347, and Ser-346 work together and function synergistically to increase the binding affinity for NADP(+)...
  75. ncbi Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate
    Zhiru Yang
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Structure 10:951-60. 2002
    ..Mutations in this binding site abolished the activating effects of fumarate. Comparison to the structure in the absence of fumarate indicates a possible molecular mechanism for the allosteric function of this compound...
  76. pmc Aggregation states of mitochondrial malate dehydrogenase
    S A Sanchez
    Instituto de Quimica, Universidad Catolica de Valparaiso, Chile
    Protein Sci 7:2184-9. 1998
    The oligomeric state of fluorescein-labeled mitochondrial malate dehydrogenase (L-malate NAD+ oxidoreductase; mMDH; EC 1.1.1...
  77. ncbi A catalytic triad is responsible for acid-base chemistry in the Ascaris suum NAD-malic enzyme
    William E Karsten
    Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, Oklahoma 73019, USA
    Biochemistry 44:3626-35. 2005
    ....
  78. ncbi Influential factor contributing to the isoform-specific inhibition by ATP of human mitochondrial NAD(P)+-dependent malic enzyme: functional roles of the nucleotide binding site Lys346
    Ju Yi Hsieh
    Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan
    FEBS J 275:5383-92. 2008
    ..Thus, ATP inhibition is proposed to be determined by the electrostatic potential involving the positive charge on the side chain of Lys346...
  79. pmc Acquired variation outweighs inherited variation in whole genome analysis of methotrexate polyglutamate accumulation in leukemia
    Deborah French
    Department of Pharmaceutical Sciences, St Jude Children s Research Hospital, Memphis, TN 38105 2794, USA
    Blood 113:4512-20. 2009
    ..We conclude that acquired genetic variation in leukemia cells has a stronger influence on MTXPG accumulation than inherited genetic variation...
  80. pmc NAD malic enzyme and the control of carbohydrate metabolism in potato tubers
    H L Jenner
    Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, United Kingdom
    Plant Physiol 126:1139-49. 2001
    ..The effect on plastid metabolism may result from the observed glycolytic perturbations...
  81. pmc Purification, cDNA cloning and heterologous expression of the human mitochondrial NADP(+)-dependent malic enzyme
    G Loeber
    Bender and Co, Vienna, Austria
    Biochem J 304:687-92. 1994
    ..Northern blot analysis showed a strong tissue-specific transcription with a predominantly high expression-rate in organs with a low division-rate...
  82. ncbi Purification, characterization, and overexpression of psychrophilic and thermolabile malate dehydrogenase of a novel antarctic psychrotolerant, Flavobacterium frigidimaris KUC-1
    Tadao Oikawa
    Department of Biotechnology, Faculty of Engineering, Kansai University, Osaka, Japan
    Biosci Biotechnol Biochem 69:2146-54. 2005
    We purified the psychrophilic and thermolabile malate dehydrogenase to homogeneity from a novel psychrotolerant, Flavobacterium frigidimaris KUC-1, isolated from Antarctic seawater...
  83. ncbi Demonstration of physical interactions between consecutive enzymes of the citric acid cycle and of the aspartate-malate shuttle. A study involving fumarase, malate dehydrogenase, citrate synthesis and aspartate aminotransferase
    S Beeckmans
    Eur J Biochem 117:527-35. 1981
    By means of covalently immobilized fumarase and mitochondrial or cytoplasmic malate dehydrogenase we were able to detect physical interactions between different enzymes of the citric acid cycle (fumarase with malate dehydrogenase, malate ..
  84. ncbi Folding of malate dehydrogenase inside the GroEL-GroES cavity
    J Chen
    Department of Chemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Nat Struct Biol 8:721-8. 2001
    ..Here, the structure of malate dehydrogenase (MDH) subunit during folding is monitored by deuterium exchange, peptic fragment production and mass ..
  85. ncbi Binding of malate dehydrogenase and NADH channelling to complex I
    J Ovadi
    Institute of Enzymology, Hungarian Academy of Sciences, Budapest
    J Mol Recognit 7:265-72. 1994
    As previously reported, mitochondrial malate dehydrogenase (MDH) binds to purified complex I of the electron transport system...
  86. ncbi Independent spontaneous mitochondrial malate dehydrogenase null mutants in soybean are the result of deletions
    J Imsande
    Department of Agronomy, Iowa State University, Ames, Iowa, USA
    J Hered 92:333-8. 2001
    The mitochondrial malate dehydrogenase-1 (Mdh1) gene of soybean [Glycine max (L.) Merr.] spontaneously mutates to a null phenotype at a relatively high rate...
  87. ncbi Non-photosynthetic 'malic enzyme' from maize: a constituvely expressed enzyme that responds to plant defence inducers
    V G Maurino
    , Universidad Nacional de Rosario, Argentina
    Plant Mol Biol 45:409-20. 2001
    ..It is deduced that the induction of housekeeping genes, like non-photosynthetic NADP-ME, whose constitutive role may be the provision of reductive power in non-photosynthetic plastids, is likely to accompany the defence response...
  88. ncbi Halophilic adaptation: novel solvent protein interactions observed in the 2.9 and 2.6 A resolution structures of the wild type and a mutant of malate dehydrogenase from Haloarcula marismortui
    S B Richard
    Institut de Biologie Structurale, CEA CNRS, 41 Avenue des Martyrs, F 38027 Grenoble Cedex 1, France
    Biochemistry 39:992-1000. 2000
    Previous biophysical studies of tetrameric malate dehydrogenase from the halophilic archaeon Haloarcula marismortui (Hm MalDH) have revealed the importance of protein-solvent interactions for its adaptation to molar salt conditions that ..
  89. ncbi Refined crystal structure of cytoplasmic malate dehydrogenase at 2.5-A resolution
    J J Birktoft
    Department of Biological Chemistry, Washington University School of Medicine, St Louis, Missouri 63110
    Biochemistry 28:6065-81. 1989
    The molecular structure of cytoplasmic malate dehydrogenase from pig heart has been refined by alternating rounds of restrained least-squares methods and model readjustment on an interactive graphics system...
  90. ncbi L-2-hydroxyglutaric aciduria, a defect of metabolite repair
    R Rzem
    de Duve Institute, Laboratory of Physiological Chemistry, Universite Catholique de Louvain, Avenue Hippocrate 75, B 1200, Brussels, Belgium
    J Inherit Metab Dis 30:681-9. 2007
    ..This enzyme co-purified with cytosolic L-malate dehydrogenase (cMDH) upon further chromatography on Blue Sepharose...
  91. pmc Regulation of malate dehydrogenase (mdh) gene expression in Escherichia coli in response to oxygen, carbon, and heme availability
    S J Park
    Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90095, USA
    J Bacteriol 177:6652-6. 1995
    b>Malate dehydrogenase catalyzes the interconversion of malate and oxaloacetate...
  92. pmc Trypanosoma cruzi mitochondrial malate dehydrogenase triggers polyclonal B-cell activation
    C L Montes
    Inmunologia, Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina
    Clin Exp Immunol 127:27-36. 2002
    ..cruzi mitochondrial malate dehydrogenase (mMDH) by enzyme activity and by Western blotting using a specific mMDH polyclonal antiserum...
  93. ncbi An NADH-tetrazolium-coupled sensitive assay for malate dehydrogenase in mitochondria and crude tissue homogenates
    Cheng Luo
    Institute for Nutritional Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, PR China
    J Biochem Biophys Methods 68:101-11. 2006
    A sensitive spectrophotometric assay for determining mitochondrial malate dehydrogenase activity is described...
  94. pmc Recognition of partially-folded mitochondrial malate dehydrogenase by GroEL. Steady and time-dependent emission anisotropy measurements
    J E Churchich
    Department of Biochemistry, University of Tennessee, Knoxville 37996 0840, USA
    Protein Sci 7:2587-94. 1998
    The binding of partially-folded mitochondrial malate dehydrogenase (mMDH) to GroEL was assessed by steady and nanosecond emission spectroscopy...
  95. doi Overproduction of C4 photosynthetic enzymes in transgenic rice plants: an approach to introduce the C4-like photosynthetic pathway into rice
    Yojiro Taniguchi
    Photobiology and Photosynthesis Research Unit, National Institute of Agrobiological Sciences NIAS, Kannondai, Tsukuba 305 8602, Japan
    J Exp Bot 59:1799-809. 2008
    ..carboxylase (PEPC), the maize C(4)-specific pyruvate, orthophosphate dikinase (PPDK), the sorghum NADP-malate dehydrogenase (MDH), and the rice C(3)-specific NADP-malic enzyme (ME), were overproduced in the mesophyll cells of rice ..
  96. pmc Decreased mitochondrial activities of malate dehydrogenase and fumarase in tomato lead to altered root growth and architecture via diverse mechanisms
    Margaretha J van der Merwe
    Max Planck Institute of Molecular Plant Physiology, D 14476 Potsdam Golm, Germany
    Plant Physiol 149:653-69. 2009
    Transgenic tomato (Solanum lycopersicum) plants in which either mitochondrial malate dehydrogenase or fumarase was antisense inhibited have previously been characterized to exhibit altered photosynthetic metabolism...
  97. ncbi Comparative study of the activity and kinetic properties of malate dehydrogenase and pyruvate decarboxylase from Candida albicans, Malassezia pachydermatis, and Saccharomyces cerevisiae
    Adam Tylicki
    Institute of Biology, University of Białystok, Swierkowa 20B, 15 950 Białystok, Poland
    Can J Microbiol 54:734-41. 2008
    ..We compare the properties of two enzymes, pyruvate decarboxylase (PDC) and malate dehydrogenase (MDH), from these species and from Saccharomyces cerevisiae cultivated under aerobic and anaerobic ..
  98. ncbi Preparation and kinetic characterization of a fusion protein of yeast mitochondrial citrate synthase and malate dehydrogenase
    C Lindbladh
    University of Lund Chemical Center, Sweden
    Biochemistry 33:11692-8. 1994
    ..In addition, the transient time for the coupled reaction sequence was less for the fusion protein than for the free enzymes...
  99. ncbi Crystal structure of a ternary complex of Escherichia coli malate dehydrogenase citrate and NAD at 1.9 A resolution
    M D Hall
    Department of Biochemistry, University of Minnesota, Minneapolis 55455
    J Mol Biol 232:213-22. 1993
    The structure of malate dehydrogenase from Escherichia coli complexed with the substrate analog, citrate and the cofactor NAD, has been determined by X-ray crystallography...
  100. ncbi Molecular cloning and functional characterization of the human cytosolic malic enzyme promoter: thyroid hormone responsiveness
    C Gonzalez-Manchon
    Department of Pathophysiology and Human Molecular Genetics, Centro de Investigaciones Biologicas CSIC, Madrid, Spain
    DNA Cell Biol 16:533-44. 1997
    ..Thus, T3 seems to control ME transcription by inducing the dissociation of TRbeta homodimers and the functional activation of liganded heterodimers...
  101. pmc Enhanced photosynthetic performance and growth as a consequence of decreasing mitochondrial malate dehydrogenase activity in transgenic tomato plants
    Adriano Nunes-Nesi
    Max Planck Institut fur Molekulare Pflanzenphysiologie, 14476 Golm, Germany
    Plant Physiol 137:611-22. 2005
    Transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the mitochondrial malate dehydrogenase gene in the antisense orientation and exhibiting reduced activity of this isoform of malate dehydrogenase show enhanced ..

Research Grants67

  1. ONTOGENY AND HORMONAL CONTROL OF CARDIAC NADH SHUTTLES
    Thomas Scholz; Fiscal Year: 2000
    ..aspartate/glutamate carrier (AGC), the oxoglutarate/malate carrier (OMC), and the mitochondrial isoforms of malate dehydrogenase, aspartate aminotransferase, and alpha-GP dehydrogenase (malpha-GPDH)...
  2. ONTOGENY AND HORMONAL CONTROL OF CARDIAC NADH SHUTTLES
    Thomas Scholz; Fiscal Year: 2001
    ..aspartate/glutamate carrier (AGC), the oxoglutarate/malate carrier (OMC), and the mitochondrial isoforms of malate dehydrogenase, aspartate aminotransferase, and alpha-GP dehydrogenase (malpha-GPDH)...
  3. ONTOGENY AND HORMONAL CONTROL OF CARDIAC NADH SHUTTLES
    Thomas Scholz; Fiscal Year: 1999
    ..aspartate/glutamate carrier (AGC), the oxoglutarate/malate carrier (OMC), and the mitochondrial isoforms of malate dehydrogenase, aspartate aminotransferase, and alpha-GP dehydrogenase (malpha-GPDH)...
  4. REGULATION OF GLYCOSOMAL BIOGENESIS IN T. BRUCEI
    C Wang; Fiscal Year: 1993
    ..in the glycosome are reduced to very low levels and replaced by phosphoenolpyruvate carboxykinase and malate dehydrogenase. We have been interested in the mechanism of protein import into the glycosome as well as the chain of ..
  5. REGULATION OF GLYCOSOMAL BIOGENESIS IN T. BRUCEI
    C Wang; Fiscal Year: 1992
    ..They and phosphoenolpyruvate carboxykinase and malate dehydrogenase of the procyclic form glycosome will be partly sequenced for gene cloning...
  6. REGULATION OF GLYCOSOMAL BIOGENESIS IN T. BRUCEI
    C Wang; Fiscal Year: 1991
    ..They and phosphoenolpyruvate carboxykinase and malate dehydrogenase of the procyclic form glycosome will be partly sequenced for gene cloning...
  7. REGULATION OF GLYCOSOMAL BIOGENESIS IN T. BRUCEI
    C Wang; Fiscal Year: 1990
    ..They and phosphoenolpyruvate carboxykinase and malate dehydrogenase of the procyclic form glycosome will be partly sequenced for gene cloning...
  8. FRUCTOFURANOSE MODULATORS--STRUCTURAL/ENZYMATIC STUDIES
    EZZAT YOUNATHAN; Fiscal Year: 1990
    ..the hypoglycemic action of 2,5-AM, two gluconeogenic enzymes, namely phosphoenolpyruvate carboxykinase and malate dehydrogenase will be investigated for sensitivity to inhibition by its bisphosphate...
  9. PROTEIN TRANSLOCATION ACROSS ORGANELLE MEMBRANES
    Arnold Strauss; Fiscal Year: 1991
    ..the common cis-acting regulatory DNA elements in human nuclear genes encoding MCAD and mitochondrial malate dehydrogenase (mMDH) which control their coordinated expression during mitochondrial biogenesis...
  10. STRUCTURE AND INTRACELLULAR LOCALIZATION OF PROTEINS
    RALPH BRADSHAW; Fiscal Year: 1990
    ..dehydrogenase, (2) obtaining full length cDNA clones for pig cytoplasmic(s) and mitochondrial (m) malate dehydrogenase (MDH), (3) obtaining a full length cdNA clone for pig Beta-hydroxyacyl Coa dehydrogenase (BetaHADH), (4) ..
  11. BIOCHEMISTRY AND PHYSIOLOGY OF SINGLE MOTOR UNITS
    PATTI NEMETH; Fiscal Year: 1990
    ..muscle fibers include: activities of enzymes (adenylokinase, creatine kinase, lactate dehydrogenase, malate dehydrogenase and beta-hydroxyacyl CoA dehydrogenase) and concentrations of phosphogens and metabolites of energy ..
  12. BIOCHEMISTRY AND PHYSIOLOGY OF SINGLE MOTOR UNITS
    PATTI NEMETH; Fiscal Year: 1993
    ..muscle fibers include: activities of enzymes (adenylokinase, creatine kinase, lactate dehydrogenase, malate dehydrogenase and beta-hydroxyacyl CoA dehydrogenase) and concentrations of phosphogens and metabolites of energy ..
  13. MITOCHONDRIAL DEVELOPMENT IN PLASMODIUM FALCIPARUM
    Naomi Lang Unnasch; Fiscal Year: 1992
    ..The marker proteins selected are cytochrome b, which is required for electron transport, mitochondrial malate dehydrogenase, which is required for the tricarboxylic acid (TCA) cycle and for the malate shuttle, and cytoplasmic ..
  14. CRYSTALLOGRAPHIC STUDIES OF PROTEIN STRUCTURE/FUNCTION
    Gregory Petsko; Fiscal Year: 1992
    ..The target enzyme, 2-isopropyl malate dehydrogenase, is essential for the biosynthesis of leucine...
  15. CRYTALLOGRAPHIC STUDIES OF PROTEIN STRUCTURE/FUNCTION
    Gregory Petsko; Fiscal Year: 1993
    ..The target enzyme, 2-isopropyl malate dehydrogenase, is essential for the biosynthesis of leucine...
  16. CRYSTALLOGRAPHIC STUDIES OF PROTEIN STRUCTURE/FUNCTION
    Gregory Petsko; Fiscal Year: 1991
    ..The target enzyme, 2-isopropyl malate dehydrogenase, is essential for the biosynthesis of leucine...
  17. CYTOSOLIC-VESICLE-VACUOLAR PROTEIN DEGRADATION PATHWAY
    Hui Ling Chiang; Fiscal Year: 2010
    ..is utilized for multiple cargo proteins including isocitrate lysase, phosphoenopyruvate carboxykinase and malate dehydrogenase. Our long-term goal is to understand the mechanisms underlying the vacuolar dependent pathway of FBPase ..
  18. EXPRESSION OF C4 GENES IN DEVELOPMENT OF MAIZE LEAF CELL
    Timothy Nelson; Fiscal Year: 1990
    ..and position-dependent expression of genes for NADP-dependent malic enzyme (ME), NADP-dependent malate dehydrogenase (MDH), phosphoenolpyruvate carboxylase (PEPCase), and their housekeeping counterparts...
  19. CYTOSOLIC-VESICLE-VACUOLAR PROTEIN DEGRADATION PATHWAY
    Hui Ling Chiang; Fiscal Year: 2009
    ..is utilized for multiple cargo proteins including isocitrate lysase, phosphoenopyruvate carboxykinase and malate dehydrogenase. Our long-term goal is to understand the mechanisms underlying the vacuolar dependent pathway of FBPase ..
  20. THEORY OF AQUEOUS TWO-PHASE EXTRACTION
    Carol Hall; Fiscal Year: 1990
    ..The proposed research is intended to provide a useful framework for the organization of existing data aqueous two-phase extraction and for the optimal design of new biorecovery processes based on this method...
  21. TRYPANOSOME GENE EXPRESSION AND ENVIRONMENTAL ADAPTATION
    Christine Clayton; Fiscal Year: 1990
    ..The first one to be attempted will be malate dehydrogenase; alternatives include phosphoenolpyruvate carboxykinase and adenylate kinase.
  22. Molecular Evolution of Pyridoxal Phoshate Enzymes
    JACK KIRSCH; Fiscal Year: 2005
    ..DNA shuffling will be used to attempt to convert an important oxidation enzyme of intermediary metabolism, malate dehydrogenase, to acquire the activity of a structurally related enzyme, lactate dehydrogenase, in order to compare the ..
  23. Molecular Evolution of Pyridoxal Phoshate Enzymes
    JACK KIRSCH; Fiscal Year: 2006
    ..DNA shuffling will be used to attempt to convert an important oxidation enzyme of intermediary metabolism, malate dehydrogenase, to acquire the activity of a structurally related enzyme, lactate dehydrogenase, in order to compare the ..
  24. CYTOSOLIC-VESICLE-VACUOLAR PROTEIN DEGRADATION PATHWAY
    Hui Ling Chiang; Fiscal Year: 2007
    ..is utilized for multiple cargo proteins including isocitrate lysase, phosphoenopyruvate carboxykinase and malate dehydrogenase. Our long-term goal is to understand the mechanisms underlying the vacuolar dependent pathway of FBPase ..
  25. ENZYMES AS CATALYSTS IN ORGANIC SYNTHESIS
    George Whitesides; Fiscal Year: 1990
    ..5) Exploration of techniques for assembling and controlling complex synthetic systems involving eight or more cooperating enzymes capable of performing both synthetic and cofactor-regenerating functions ("artificial metabolism")...
  26. GROEL-MEDIATED PROTEIN FOLDING
    Arthur Horwich; Fiscal Year: 1999
    ..to characterize conformational changes which accompany the binding of metastable rubisco and mitochondrial malate dehydrogenase (mMDH) forms to GroEL...
  27. GROEL-MEDIATED PROTEIN FOLDING
    Arthur Horwich; Fiscal Year: 2000
    ..to characterize conformational changes which accompany the binding of metastable rubisco and mitochondrial malate dehydrogenase (mMDH) forms to GroEL...
  28. MOLECULAR GENETICS OF MALATE DEHYDROGENASE ISOZYMES
    Lee McAlister Henn; Fiscal Year: 1993
    ..Our goal is to determine the role of isozymes of malate dehydrogenase in regulating metabolic flux within and across compartmental barriers...
  29. MOLECULAR ANALYSIS OF THE TRYPANOSOME GLYCOSOME
    Marilyn Parsons; Fiscal Year: 1991
    ..The work on gene regulation will be expanded to include a procyclic-specific glycosomal protein, malate dehydrogenase. The corresponding gene will be cloned, and the regulation of its expression investigated...
  30. STRUCTURE AND FUNCTION OF DEHYDROGENASES
    RALPH BRADSHAW; Fiscal Year: 1980
    Amino acid sequence analyses of porcine heart mitochondrial malate dehydrogenase (mMDH) and Beta-hydroxyacyl CoA dehydrogenase (Beta HADH) are in progress...
  31. MOLECULAR ANALYSIS OF THE TRYPANOSOME GLYCOSOME
    Marilyn Parsons; Fiscal Year: 1992
    ..The work on gene regulation will be expanded to include a procyclic-specific glycosomal protein, malate dehydrogenase. The corresponding gene will be cloned, and the regulation of its expression investigated...
  32. MOLECULAR ANALYSIS OF THE TRYPANOSOME GLYCOSOME
    Marilyn Parsons; Fiscal Year: 1993
    ..The work on gene regulation will be expanded to include a procyclic-specific glycosomal protein, malate dehydrogenase. The corresponding gene will be cloned, and the regulation of its expression investigated...
  33. PROTEIN TRANSLOCATION ACROSS ORGANELLE MEMBRANES
    Arnold Strauss; Fiscal Year: 1990
    ..The model for this post-translational uptake is the biosynthesis of rat mitochondrial malate dehydrogenase and glutamate dehydrogenase...
  34. MOLECULAR ANALYSIS OF THE TRYPANOSOME GLYCOSOME
    Marilyn Parsons; Fiscal Year: 1990
    ..The work on gene regulation will be expanded to include a procyclic-specific glycosomal protein, malate dehydrogenase. The corresponding gene will be cloned, and the regulation of its expression investigated...
  35. REGULATION OF ENZYME LEVELS IN MOUSE HEPATOMA
    Michael Felder; Fiscal Year: 1980
    ..and 6-phosphogluconate dehydrogenases, are increased in fetal liver and hepatoma, while NAD-malate dehydrogenase, aspartate aminotransferase, alcohol dehydrogenase and NADP-isocitrate dehydrogenase are decreased in ..
  36. PROTEIN TRANSLOCATION ACROSS ORGANELLE MEMBRANES
    Arnold Strauss; Fiscal Year: 1980
    ..the secretory protein, rat liver prothrombin; and the mitochondrial enzymes, glutamate dehydrogenase and malate dehydrogenase, to study the translocation of these cytoplasmically-synthesized proteins across endoplasmic reticular and ..
  37. MOLECULAR GENETICS OF MALATE DEHYDROGENASE ISOZYMES
    Lee McAlister Henn; Fiscal Year: 1990
    ..Our goal is to examine the role of compartmentalized isozymes of malate dehydrogenase in regulating the exchange of oxaloacetate and NAD(H) through the shuttle of malate...
  38. EXPRESSION OF C4 GENES IN DEVELOPMENT OF LEAF CELL TYPES
    Timothy Nelson; Fiscal Year: 1992
    ..position-dependent expression of genes for NADP-dependent malic enzyme (ME, BS-specific) and NADP-dependent malate dehydrogenase (MDH, M-specific), using expression assays in protoplasts and intact leaves, and (3) to determine whether ..
  39. STRUCTURAL STUDIES OF LIPID:PROTEIN SYSTEMS
    Leonard Banaszak; Fiscal Year: 1992
    ..involves single crystal x-ray studies of another recombinant protein, the precursor form of mitochondrial malate dehydrogenase, premMDH. PremMDH is the form of malate dehydrogenase which is biosynthesized in cytosol...
  40. STRUCTURAL STUDIES OF LIPID:PROTEIN SYSTEMS
    Leonard Banaszak; Fiscal Year: 1990
    ..involves single crystal x-ray studies of another recombinant protein, the precursor form of mitochondrial malate dehydrogenase, premMDH. PremMDH is the form of malate dehydrogenase which is biosynthesized in cytosol...
  41. STRUCTURAL STUDIES OF LIPID:PROTEIN SYSTEMS
    Leonard Banaszak; Fiscal Year: 1993
    ..involves single crystal x-ray studies of another recombinant protein, the precursor form of mitochondrial malate dehydrogenase, premMDH. PremMDH is the form of malate dehydrogenase which is biosynthesized in cytosol...
  42. STRUCTURAL STUDIES OF LIPID:PROTEIN SYSTEMS
    Leonard Banaszak; Fiscal Year: 1991
    ..involves single crystal x-ray studies of another recombinant protein, the precursor form of mitochondrial malate dehydrogenase, premMDH. PremMDH is the form of malate dehydrogenase which is biosynthesized in cytosol...
  43. Mechanisms of Nucleic Acid Uptake and The Role of cMDH
    Basil Hanss; Fiscal Year: 2006
    ..We have identified four protein components of the channel. One of which, cytosolic malate dehydrogenase, functions as the regulatory subunit of the channel...
  44. Mechanisms of Nucleic Acid Uptake and The Role of cMDH
    Basil Hanss; Fiscal Year: 2007
    ..We have identified four protein components of the channel. One of which, cytosolic malate dehydrogenase, functions as the regulatory subunit of the channel...
  45. Mechanisms of Nucleic Acid Uptake and The Role of cMDH
    Basil Hanss; Fiscal Year: 2005
    ..We have identified four protein components of the channel. One of which, cytosolic malate dehydrogenase, functions as the regulatory subunit of the channel...
  46. Survivin Regulation of Apoptosis
    Nathan Wall; Fiscal Year: 2003
    ..intramitochondrial space to the cytosol of cytochrome C; and the release from the mitochondrial matrix of malate dehydrogenase (MDH)...
  47. Mechanisms of Nucleic Acid Uptake and The Role of cMDH
    Basil Hanss; Fiscal Year: 2004
    ..We have identified four protein components of the channel. One of which, cytosolic malate dehydrogenase, functions as the regulatory subunit of the channel...
  48. ENZYME STRUCTURE OF ACYL-COA BINDING SITES
    GARY POWELL; Fiscal Year: 1980
    ..Biophys. Res. commun. 76 (1977) 843-849. The interactions of these analogues with citrate synthase and malate dehydrogenase will be characterized using activity measurements, radiotracers, and by competitive binding studies using ..
  49. STRUCTURAL STUDIES OF DEHYDROGENASES AND LIPOPROTEINS
    Leonard Banaszak; Fiscal Year: 1980
    Single crystal X-ray analysis will be continued on the enzyme:mitochondrial malate dehydrogenase (m-MDH). Two monoclinic forms, labelled A and B, are being studied...
  50. STRUCTURAL PROPERTIES OF MALATE DEHYDROGENASE
    John Harrison; Fiscal Year: 1980
    ..of this project has been to attempt to understand the structure-function relationships in the two forms of malate dehydrogenase from porcine heart...
  51. METABOLIC REGULATION AND INTERACTING ENZYME SYSTEMS
    Carl Frieden; Fiscal Year: 1980
    ..We are also studying the effect of acyl CoA derivatives on enzymes such as malate dehydrogenase and citrate synthase. In addition, we are carrying out chemical modification studies on muscle actin.