3 hydroxysteroid dehydrogenases

Summary

Summary: Catalyze the oxidation of 3-hydroxysteroids to 3-ketosteroids.

Top Publications

  1. ncbi Alastrim smallpox variola minor virus genome DNA sequences
    S N Shchelkunov
    Department of Molecular Biology of Genomes, State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Region, 633159, Russia
    Virology 266:361-86. 2000
  2. ncbi The development of rat Leydig cell progenitors in vitro: how essential is luteinising hormone?
    Katja J Teerds
    Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    J Endocrinol 194:579-93. 2007
  3. ncbi Selective reduction of AKR1C2 in prostate cancer and its role in DHT metabolism
    Qing Ji
    Division of Gastrointestinal and Liver Disease, Keck School of Medicine, University of Southern California, Los Angeles, California, USA
    Prostate 54:275-89. 2003
  4. ncbi Molecular cloning of human type 3 3 alpha-hydroxysteroid dehydrogenase that differs from 20 alpha-hydroxysteroid dehydrogenase by seven amino acids
    I Dufort
    Medical Research Council Group in Molecular Endocrinology, CHUL Research Center, Quebec, Canada
    Biochem Biophys Res Commun 228:474-9. 1996
  5. ncbi Characteristics of a highly labile human type 5 17beta-hydroxysteroid dehydrogenase
    I Dufort
    Medical Research Council Group in Molecular Endocrinology, CHUL Research Center and Laval University, Quebec, Quebec, Canada
    Endocrinology 140:568-74. 1999
  6. ncbi Equine P450 cholesterol side-chain cleavage and 3 beta-hydroxysteroid dehydrogenase/delta(5)-delta(4) isomerase: molecular cloning and regulation of their messenger ribonucleic acids in equine follicles during the ovulatory process
    D Boerboom
    Centre de recherche en reproduction animale and Département de biomédecine vétérinaire, Faculte de Medecine Veterinaire, Universite de Montreal, C P 5000, Saint Hyacinthe, Quebec, Canada J2S 7C6
    Biol Reprod 64:206-15. 2001
  7. ncbi The aldo-keto reductase AKR1C3 contributes to 7,12-dimethylbenz(a)anthracene-3,4-dihydrodiol mediated oxidative DNA damage in myeloid cells: implications for leukemogenesis
    Jane Birtwistle
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Mutat Res 662:67-74. 2009
  8. ncbi cDNA cloning and expression of the human hepatic bile acid-binding protein. A member of the monomeric reductase gene family
    A Stolz
    Division of Gastrointestinal and Liver Diseases, University of Southern California School of Medicine, Los Angeles 90033
    J Biol Chem 268:10448-57. 1993
  9. ncbi Expression of 3beta-hydroxysteroid dehydrogenase type I and type VI isoforms in the mouse testis during development
    P J Baker
    Division of Veterinary Physiology, Department of Veterinary Pre clinical Studies, University of Glasgow Veterinary School, UK
    Eur J Biochem 260:911-7. 1999
  10. ncbi 5alpha-reductases in human breast carcinoma: possible modulator of in situ androgenic actions
    T Suzuki
    Department of Pathology, Tohoku University School of Medicine, Tohoku University Hospital N A, T M, Sendai 980 8575, Japan
    J Clin Endocrinol Metab 86:2250-7. 2001

Detail Information

Publications160 found, 100 shown here

  1. ncbi Alastrim smallpox variola minor virus genome DNA sequences
    S N Shchelkunov
    Department of Molecular Biology of Genomes, State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Region, 633159, Russia
    Virology 266:361-86. 2000
    ..The former segment aligns well with sequences in other orthopoxviruses, particularly cowpox and vaccinia viruses, and the latter is apparently alastrim-specific...
  2. ncbi The development of rat Leydig cell progenitors in vitro: how essential is luteinising hormone?
    Katja J Teerds
    Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    J Endocrinol 194:579-93. 2007
    ..When interstitial cells were isolated from 13-day-old rats and to a lesser extent from 10-day-old rats, a small proportion of the precursors could develop into progenitor cells independent of the presence of LH...
  3. ncbi Selective reduction of AKR1C2 in prostate cancer and its role in DHT metabolism
    Qing Ji
    Division of Gastrointestinal and Liver Disease, Keck School of Medicine, University of Southern California, Los Angeles, California, USA
    Prostate 54:275-89. 2003
    ..Functional activity and regulation of AKR1C2, a 3 alpha-hydroxysteroid dehydrogenase (HSD) type III, was also assessed in prostate cancer cell lines...
  4. ncbi Molecular cloning of human type 3 3 alpha-hydroxysteroid dehydrogenase that differs from 20 alpha-hydroxysteroid dehydrogenase by seven amino acids
    I Dufort
    Medical Research Council Group in Molecular Endocrinology, CHUL Research Center, Quebec, Canada
    Biochem Biophys Res Commun 228:474-9. 1996
    ..1, 78.3, and 67.4% identity with rat 3 alpha-HSD and rabbit and rat 20 alpha-HSD, respectively. 3 alpha-HSD3 belongs to the aldo-keto reductase family and like almost all the members of this family preferred NADPH as cofactor...
  5. ncbi Characteristics of a highly labile human type 5 17beta-hydroxysteroid dehydrogenase
    I Dufort
    Medical Research Council Group in Molecular Endocrinology, CHUL Research Center and Laval University, Quebec, Quebec, Canada
    Endocrinology 140:568-74. 1999
    ....
  6. ncbi Equine P450 cholesterol side-chain cleavage and 3 beta-hydroxysteroid dehydrogenase/delta(5)-delta(4) isomerase: molecular cloning and regulation of their messenger ribonucleic acids in equine follicles during the ovulatory process
    D Boerboom
    Centre de recherche en reproduction animale and Département de biomédecine vétérinaire, Faculte de Medecine Veterinaire, Universite de Montreal, C P 5000, Saint Hyacinthe, Quebec, Canada J2S 7C6
    Biol Reprod 64:206-15. 2001
    ..Thus, this study characterizes for the first time the complete structure of equine P450(scc) and 3 beta-HSD mRNA and identifies novel patterns of expression and regulation of these transcripts in equine follicles prior to ovulation...
  7. ncbi The aldo-keto reductase AKR1C3 contributes to 7,12-dimethylbenz(a)anthracene-3,4-dihydrodiol mediated oxidative DNA damage in myeloid cells: implications for leukemogenesis
    Jane Birtwistle
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Mutat Res 662:67-74. 2009
    ....
  8. ncbi cDNA cloning and expression of the human hepatic bile acid-binding protein. A member of the monomeric reductase gene family
    A Stolz
    Division of Gastrointestinal and Liver Diseases, University of Southern California School of Medicine, Los Angeles 90033
    J Biol Chem 268:10448-57. 1993
    ..Future studies will define the physiologic significance of this novel, multifunctional protein in bile acid transport and xenobiotic metabolism...
  9. ncbi Expression of 3beta-hydroxysteroid dehydrogenase type I and type VI isoforms in the mouse testis during development
    P J Baker
    Division of Veterinary Physiology, Department of Veterinary Pre clinical Studies, University of Glasgow Veterinary School, UK
    Eur J Biochem 260:911-7. 1999
    ..The limited expression of the type VI isoform means that it will be a useful marker in studies of adult Leydig cell development...
  10. ncbi 5alpha-reductases in human breast carcinoma: possible modulator of in situ androgenic actions
    T Suzuki
    Department of Pathology, Tohoku University School of Medicine, Tohoku University Hospital N A, T M, Sendai 980 8575, Japan
    J Clin Endocrinol Metab 86:2250-7. 2001
    ....
  11. ncbi Immunoelectron microscopic localization of three key steroidogenic enzymes (cytochrome P450(scc), 3 beta-hydroxysteroid dehydrogenase and cytochrome P450(c17)) in rat adrenal cortex and gonads
    G Pelletier
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL, and Laval University, Quebec, Canada G1V 4G2
    J Endocrinol 171:373-83. 2001
    ....
  12. ncbi Changes in Leydig cell gene expression during development in the mouse
    P J O'Shaughnessy
    Division of Veterinary Physiology and Pharmacology, Department of Veterinary Preclinical Studies, University of Glasgow Veterinary School, Bearsden Road, Glasgow G61 1QH, United Kingdom
    Biol Reprod 66:966-75. 2002
    ..The developmental pattern of gene expression suggests that three distinct phases of adult Leydig cell differentiation occur...
  13. ncbi The aldo-keto reductase AKR1C3 is a novel suppressor of cell differentiation that provides a plausible target for the non-cyclooxygenase-dependent antineoplastic actions of nonsteroidal anti-inflammatory drugs
    Julian C Desmond
    Division of Medical Sciences, University of Birmingham, Birmingham, B15 2TH United Kingdom
    Cancer Res 63:505-12. 2003
    ..In addition, these observations identify AKR1C3 as plausible target for the non-cyclooxygenase-dependent antineoplastic actions of NSAIDs...
  14. ncbi Potentiation of myeloid differentiation by anti-inflammatory agents, by steroids and by retinoic acid involves a single intracellular target, probably an enzyme of the aldoketoreductase family
    C M Bunce
    Department of Immunology, University of Birmingham, Edgbaston, UK
    Biochim Biophys Acta 1311:189-98. 1996
    ....
  15. ncbi Structural basis of the multispecificity demonstrated by 17beta-hydroxysteroid dehydrogenase types 1 and 5
    S X Lin
    Molecular Endocrinology Research Center at Laval University Hospital Research Center CHUL, CHUQ, Laval University, Que, Canada G1V 4G2
    Mol Cell Endocrinol 248:38-46. 2006
    ..The multi-specificity contributes significantly to steroid metabolism in peripheral tissues, due to the high levels of 17beta-HSD5 mRNA in both breast and prostate tissues...
  16. ncbi Substrate specificity, gene structure, and tissue-specific distribution of multiple human 3 alpha-hydroxysteroid dehydrogenases
    M Khanna
    Department of Pediatrics, Cornell University Medical College, New York, New York 10021, USA
    J Biol Chem 270:20162-8. 1995
    ..The type I gene contains a TATA box that is located 27 bp upstream of multiple transcription start sites. In contrast, the type II gene contains two tandem AP2 sequences juxtaposed to a single transcription start site...
  17. pmc Human 3alpha-hydroxysteroid dehydrogenase isoforms (AKR1C1-AKR1C4) of the aldo-keto reductase superfamily: functional plasticity and tissue distribution reveals roles in the inactivation and formation of male and female sex hormones
    T M Penning
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6084, USA
    Biochem J 351:67-77. 2000
    ..These studies are in stark contrast with those in rat where only a single AKR with positional- and stereo-specificity for 3alpha-hydroxysteroids exists...
  18. pmc In search of rat stem Leydig cells: identification, isolation, and lineage-specific development
    Ren Shan Ge
    Population Council, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 103:2719-24. 2006
    ..We conclude that these cells are likely to be the sought-after SLCs...
  19. ncbi Molecular characterization of postnatal development of testicular steroidogenesis in luteinizing hormone receptor knockout mice
    Fu Ping Zhang
    Department of Physiology, Institute of Biomedicine, University of Turku, Finland
    Endocrinology 145:1453-63. 2004
    ....
  20. ncbi Human types 1 and 3 3 alpha-hydroxysteroid dehydrogenases: differential lability and tissue distribution
    I Dufort
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL and Laval University, Quebec G1V 4G2, Canada
    J Clin Endocrinol Metab 86:841-6. 2001
    ....
  21. ncbi Dual subcellular localization of the 3 beta-hydroxysteroid dehydrogenase isomerase: characterization of the mitochondrial enzyme in the bovine adrenal cortex
    N Cherradi
    Unité INSERM 244, DBMS BRCE, Centre d Etudes Nucléaires, Grenoble, France
    J Steroid Biochem Mol Biol 46:773-9. 1993
    ....
  22. ncbi Identification of the oxidative 3alpha-hydroxysteroid dehydrogenase activity of rat Leydig cells as type II retinol dehydrogenase
    D O Hardy
    Population Council and The Rockefeller University, New York, New York 10021, USA
    Endocrinology 141:1608-17. 2000
    ..We conclude that type I 3alpha-HSD and RoDH2 have distinct 3alpha-HSD activities with opposing catalytic directions, thereby controlling the rates of DHT production by Leydig cells...
  23. ncbi Deletion of fowlpox virus homologues of vaccinia virus genes between the 3 beta-hydroxysteroid dehydrogenase (A44L) and DNA ligase (A50R) genes
    M A Skinner
    Institute for Animal Health, Compton Laboratory, Nr Newbury, Berkshire, U K
    J Gen Virol 75:2495-8. 1994
    ..Increased 3 beta-HSD activity has been demonstrated in cells infected with either of two different strains of FPV or with canarypox virus...
  24. ncbi Development of the adult-type Leydig cell population in the rat is affected by neonatal thyroid hormone levels
    K J Teerds
    Department of Cell Biology and Histology, Faculty of Veterinary Medicine, Utrecht University, 3508 TD Utrecht, The Netherlands
    Biol Reprod 59:344-50. 1998
    ....
  25. ncbi Structure-function relationships of 3 beta-hydroxysteroid dehydrogenase: contribution made by the molecular genetics of 3 beta-hydroxysteroid dehydrogenase deficiency
    Y Morel
    INSERM U329, Department of Pediatrics, Universite de Lyon, Hopital Debrousse, Hospices Civils de Lyon, France
    Steroids 62:176-84. 1997
    ....
  26. ncbi Changes in mRNAs encoding steroidogenic acute regulatory protein, steroidogenic enzymes and receptors for gonadotropins during spermatogenesis in rainbow trout testes
    M Kusakabe
    School of Aquatic and Fishery Sciences, University of Washington, 1140 Boat Street NE, Seattle, Washington 98195 5020, USA
    J Endocrinol 189:541-54. 2006
    ....
  27. ncbi Influence of ovarian ornithine decarboxylase in folliculogenesis and luteinization
    Carmen M Bastida
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, Campus de Espinardo, 30100 Murcia, Spain
    Endocrinology 146:666-74. 2005
    ....
  28. ncbi A hepatocarcinogenic tryptophan-pyrolyzate component, Trp-P-1, decreases serum total testosterone level and induces hepatic Cyp1a2 in male mice
    Masakuni Degawa
    Department of Molecular Toxicology and the COE Program in the 21st Century, School of Pharmaceutical Sciences, University of Shizuoka, 52 1 Yada, Suruga ku, Shizuoka 422 8526, Japan
    Cancer Sci 97:32-7. 2006
    ....
  29. ncbi Hormonally active nontransformed human ovarian cell culture from oophorectomy specimens: methods of development and initial characterization
    Donna Seto-Young
    Division of Endocrinology, Department of Medicine, Beth Israel Medical Center and Albert Einstein College of Medicine, NY 10003, USA
    Horm Res 64:238-47. 2005
    ....
  30. ncbi Spermatogenesis, serum testosterone levels and immunolocalization of steroidogenic enzymes in the wild male Japanese black bear (Ursus thibetanus japonicus)
    Tsukasa Okano
    United Graduate School of Veterinary Medicine, Gifu University
    J Vet Med Sci 65:1093-9. 2003
    ..Leydig cells, Sertoli cells and germ cells have the capability of synthesizing androgen, and Leydig cells, Sertoli cells, spermatids and spermatogonia have the capability of synthesizing estrogen in Japanese black bears...
  31. ncbi Suppression of steroidogenic enzyme expression during androgen-induced sex reversal in Nile tilapia (Oreochromis niloticus)
    Ramji Kumar Bhandari
    Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, 3422 Sesoko, Motobu, Okinawa 905 0227, Japan
    Gen Comp Endocrinol 145:20-4. 2006
    ..Whether the absence of aromatase or the presence of androgens is responsible for testicular differentiation remains to be determined...
  32. ncbi Inhibition of CYP450scc expression in dioxin-exposed rat Leydig cells
    K P Lai
    Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, People s Republic of China
    J Endocrinol 185:519-27. 2005
    ..The data indicate that TCDD can modulate cAMP signaling in rat Leydig cells to affect the process of steroidogenesis...
  33. ncbi Dose-dependent alterations in gene expression and testosterone synthesis in the fetal testes of male rats exposed to di (n-butyl) phthalate
    Kim P Lehmann
    CIIT Centers for Health Research, Research Triangle Park, NC 27709, USA
    Toxicol Sci 81:60-8. 2004
    ..Alterations in gene and protein expression and testosterone synthesis may serve as sensitive indicators of testicular response to DBP...
  34. ncbi Blocking BRE expression in Leydig cells inhibits steroidogenesis by down-regulating 3beta-hydroxysteroid dehydrogenase
    J Miao
    Department of Surgery, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, People s Republic of China
    J Endocrinol 185:507-17. 2005
    ..Our results suggest that BRE influences steroidogenesis through its effects on 3beta-HSD action, probably affecting its transcription...
  35. ncbi Differential actions of metyrapone on the fetal pituitary-adrenal axis in the sheep fetus in late gestation
    K E Warnes
    Department of Physiology, University of Adelaide, Adelaide, South Australia 5000, Australia
    Biol Reprod 71:620-8. 2004
    ..Thus, metyrapone administration may represent a unique model that allows the investigation of dissociation of the relative actions of ACTH and cortisol on fetal adrenal steroidogenesis and growth during late gestation...
  36. ncbi Cattle and sheep use different promoters to direct the expression of the aromatase cytochrome P450 encoding gene, Cyp19, during pregnancy
    Jens Vanselow
    Research Institute for the Biology of Farm Animals, Research Unit Molecular Biology, Wilhelm Stahl Allee 2, 18196 Dummerstorf, Germany
    Domest Anim Endocrinol 27:99-114. 2004
    ..1 derived transcripts. Contrary to the bovine, the sheep corpus luteum also showed considerable P1.5 derived expression. This demonstrates that cattle and sheep use different promoters to direct Cyp19 expression during pregnancy...
  37. pmc Molecular mechanism of suppression of testicular steroidogenesis by proinflammatory cytokine tumor necrosis factor alpha
    Cheol Yi Hong
    Hormone Research Center, School of Biological Sciences and Technology, Department of Biology, Chonnam National University, Gwangju 500 757, Republic of Korea
    Mol Cell Biol 24:2593-604. 2004
    ..These findings provide a molecular mechanism underlying the inhibition of testicular steroidogenesis by proinflammatory cytokines...
  38. ncbi Immunolocalization of steroidogenic enzymes in the corpus luteum and placenta of the Japanese Shiba goat
    Qiang Weng
    Faculty of Biological Science and Technology, Beijing Forestry University, China
    J Reprod Dev 51:247-52. 2005
    ..Also the placenta has the ability to synthesize androgen and estrogen in late pregnancy...
  39. ncbi Immunolocalization of steroidogenic enzymes P450scc, 3betaHSD, P450c17, and P450arom in Göttingen miniature pig testes
    Qiang Weng
    Faculty of Biological Science and Technology, Beijing Forestry University, China
    J Reprod Dev 51:299-304. 2005
    ..These results suggested that adult GM pig testes have the ability to produce germ cells throughout the year, and the synthesis of progestin, androgen and estrogen occurs in the Leydig cells of GM pig testes...
  40. ncbi Cyclophosphamide treatment causes impairment of sperm and its fertilizing ability in mice
    Namasivayam Elangovan
    Institute of Biochemical Science, College of Life Science, National Taiwan University, Taipei, Taiwan, ROC
    Toxicology 222:60-70. 2006
    ..These results demonstrate that cyclophosphamide caused temporary interference of normal male reproductive system with low dose treatment, but might be permanent dysfunction in high dose treatment...
  41. ncbi Radiation exposure impairs luteinizing hormone signal transduction and steroidogenesis in cultured human leydig cells
    Ramadoss Sivakumar
    Department of Endocrinology, Dr ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, Tamil Nadu, India
    Toxicol Sci 91:550-6. 2006
    ..Decreased level of LH receptors following higher doses of radiation exposure is not coupled with impaired expression of its mRNA...
  42. ncbi Effects of dietary fats and proteins on rat testicular steroidogenic enzymes and serum testosterone levels
    Mark J McVey
    Toxicology Research Division, Health Products and Foods Branch, Food Directorate, Health Canada, Sir Fredrick G Banting Research Centre, 2202D1 Tunney s Pasture, Ottawa, ON, Canada K1A 0L2
    Food Chem Toxicol 46:259-69. 2008
    ....
  43. ncbi Ascorbic acid transported by sodium-dependent vitamin C transporter 2 stimulates steroidogenesis in human choriocarcinoma cells
    Ximei Wu
    Department of Toxicology, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Endocrinology 149:73-83. 2008
    ..Our observations indicate that SVCT2-mediated AA uptake into cells is necessary for AA-induced steroidogenesis in human choriocarcinoma cell, but MAPK kinase-ERK signaling is not involved in AA-induced steroidogenesis...
  44. ncbi Strontium fructose 1,6-diphosphate rescues adenine-induced male hypogonadism and upregulates the testicular endothelin-1 system
    Yu Feng
    Research Division of Pharmacology, China Pharmaceutical University, Nanjing, China
    Clin Exp Pharmacol Physiol 34:1131-7. 2007
    ..These abnormalities were significantly restored by FDP-Sr in a dose-dependent manner. These data indicate the possibility of using FDP-Sr to treat male hypogonadism...
  45. ncbi An investigation of the effects of methylmercury in rats fed different dietary fats and proteins: testicular steroidogenic enzymes and serum testosterone levels
    Mark J McVey
    Toxicology Research Division, Health Products and Foods Branch, Food Directorate, Health Canada, Sir Fredrick G Banting Research Centre, 2202D1 Tunney s Pasture, Ottawa, ON, Canada K1A 0L2
    Food Chem Toxicol 46:270-9. 2008
    ..In conclusion, these studies show that different lipid diets can alter the toxic effects of MeHg on male rat steroidogenesis in terms of serum testosterone and steroidogenic enzyme activities...
  46. ncbi Developmental expression of key steroidogenic enzymes in the brain of protandrous black porgy fish, Acanthopagrus schlegeli
    S Tomy
    Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan
    J Neuroendocrinol 19:643-55. 2007
    ..The study suggests an important role for brain aromatase in male black porgy brain sex differentiation, and considers the possibility of a role for this enzyme in neurogenesis...
  47. ncbi The expression of ovine placental lactogen, StAR and progesterone-associated steroidogenic enzymes in placentae of overnourished growing adolescent ewes
    Richard G Lea
    School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Sutton Bonington LE12 5RD, UK
    Reproduction 133:785-96. 2007
    ..Restricted placental growth and reduced biosynthesis may account for reduced progesterone in day 130 H+IUGR ewes...
  48. ncbi Fibroblast growth factor-9, a local regulator of ovarian function
    Ann E Drummond
    Prince Henry s Institute of Medical Research, PO Box 5152, Clayton, Victoria 3168, Australia
    Endocrinology 148:3711-21. 2007
    ..In summary, the data are consistent with a paracrine role for FGF9 in the ovary...
  49. ncbi Modulation of antioxidant defense system by the environmental fungicide carbendazim in Leydig cells of rats
    Sivasankaran Rajeswary
    Department of Zoology, Kanchi Mamunivar Centre for Post Graduate Studies, Tagore Arts College Campus, Lawspet, Pondicherry, India
    Reprod Toxicol 24:371-80. 2007
    ..Thus, the present study suggests that chronic low dose treatment of MBC is capable of inducing reproductive toxicity through increased oxidative stress, but is transient and reversible upon withdrawal of treatment...
  50. ncbi Production of sex steroid hormones from DHEA in articular chondrocyte of rats
    Satsuki Takeuchi
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tennodai 1 1 1, Tsukuba, Ibaraki 305 8574, Japan
    Am J Physiol Endocrinol Metab 293:E410-5. 2007
    ..These findings provided the first evidence that articular chondrocytes expressed steroidogenesis-related enzyme genes and that they are capable of locally synthesizing sex steroid hormones locally from DHEA...
  51. ncbi Elevated expression of P450c17 (CYP17) during testicular formation in the frog
    Ryosuke Iwade
    Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, 1 6 1 Nishi Waseda, Shinjuku ku, Tokyo 169 8050, Japan
    Gen Comp Endocrinol 155:79-87. 2008
    ..Taken together, the results suggest that CYP17 is very involved in testicular differentiation of the gonad in R. rugosa...
  52. ncbi Alterations in gene expression and testosterone synthesis in the testes of male rats exposed to perfluorododecanoic acid
    Zhimin Shi
    Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Toxicol Sci 98:206-15. 2007
    ..Our results demonstrate that PFDoA affects the reproduction function of male rats via alterations in steroidogenesis genes, testosterone levels, and testes ultrastructure...
  53. ncbi Study on the mechanism of trichlorfon-induced inhibition of progesterone synthesis in mouse leydig tumor cells (MLTC-1)
    Xia Hong
    Key Laboratory of Reproductive Medicine of Jiangsu Province, Institute of Toxicology, Nanjing Medical University, Nanjing, PR China
    Toxicology 234:51-8. 2007
    ..These results indicated that trichlorfon suppressed progesterone synthesis in MLTC-1 cells, at least in part, via inhibiting StAR expression and P450scc activity...
  54. ncbi Effects of vitamins C and E on steroidogenic enzymes mRNA expression in polychlorinated biphenyl (Aroclor 1254) exposed adult rat Leydig cells
    Palaniappan Murugesan
    Department of Endocrinology, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India
    Toxicology 232:170-82. 2007
    ..These observations suggest that vitamins C and E have ameliorative role against PCBs-induced testicular Leydig cells dysfunction...
  55. ncbi Variants in estrogen biosynthesis genes, sex steroid hormone levels, and endometrial cancer: a HuGE review
    Sara H Olson
    Department of Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, New York, NY, USA
    Am J Epidemiol 165:235-45. 2007
    ..Larger studies or combined studies that allow for investigation of gene-gene and gene-environment interactions are warranted...
  56. ncbi Liver receptor homolog-1 and steroidogenic factor-1 have similar actions on rat granulosa cell steroidogenesis
    Deeksha Saxena
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, 830 Scaife Hall, 3500 Terrace Street, Pittsburgh, Pennsylvania 15261, USA
    Endocrinology 148:726-34. 2007
    ....
  57. ncbi cAMP-independent signaling regulates steroidogenesis in mouse Leydig cells in the absence of StAR phosphorylation
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    J Mol Endocrinol 37:81-95. 2006
    ..Collectively, these findings demonstrate that, in mouse Leydig cells, cAMP-independent signaling events regulate steroidogenesis in a StAR phosphorylation-independent manner...
  58. ncbi Oxygen concentration is an important factor for modulating progesterone synthesis in bovine corpus luteum
    Ryo Nishimura
    Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    Endocrinology 147:4273-80. 2006
    ....
  59. doi Role of IGFs and insulin in the human testis during postnatal activation: differentiation of steroidogenic cells
    Esperanza B Berensztein
    Endocrine Service, Hospital de Pediatria Garrahan, Buenos Aires C1245 AAM, Argentina
    Pediatr Res 63:662-6. 2008
    ..In some interstitial cells, probably in LC precursors, IGF-II/IR could be involved, among other factors, in the stimulation of PI and/or inhibition of AI, and in LC differentiation...
  60. ncbi Influence of cytokines and growth factors on distinct steroidogenic enzymes in vitro: a short tabular data collection
    M Herrmann
    Institute of Sports and Preventive Medicine, University of Saarland, 66041 Saarbrucken, Germany
    Ann N Y Acad Sci 966:166-86. 2002
    ..This collection summarizes the current information on how the mentioned cytokines and growth factors influence particular enzyme steps...
  61. ncbi Effects of amphetamine on steroidogenesis in MA-10 mouse Leydig tumor cells
    Liang Yu Chen
    Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    Life Sci 72:1983-95. 2003
    ..05). These results suggested that amphetamine enhanced hCG-induced progesterone production in MA-10 cells by increasing P450scc activity without influencing StAR protein and P450scc enzyme expression or 3beta-HSD enzyme activity...
  62. ncbi Assessment of mechanisms of thyroid hormone action in mouse Leydig cells: regulation of the steroidogenic acute regulatory protein, steroidogenesis, and luteinizing hormone receptor function
    P R Manna
    Department of Physiology, Institute of Biomedicine, University of Turku, FIN-20520 Turku, Finland
    Endocrinology 142:319-31. 2001
    ..Collectively, these observations further characterize the thyroid-gonadal connection and provide insights into the mechanisms for a dual regulatory role of thyroid hormone in Leydig cell functions...
  63. ncbi RU-486 inhibits rat gonadal steroidogenesis
    P E Sanchez
    Developmental Endocrinology Branch, National Institute of Child Health and Human Development, Bethesda, Maryland
    Horm Metab Res 21:369-71. 1989
    ..Ovarian 17-hydroxylase but not 3 beta-hydroxysteroid dehydrogenase activity was decreased in the animals receiving the drug. We conclude that RU-486 inhibits both testicular and ovarian steroidogenesis in the rat...
  64. ncbi Localization of type 1 17beta-hydroxysteroid dehydrogenase mRNA and protein in syncytiotrophoblasts and invasive cytotrophoblasts in the human term villi
    M Bonenfant
    Laboratory of Ontogeny and Reproduction, Centre Hospitalier Universitaire de Quebec, Pavillon CHUL, Canada G1V 4G2
    J Endocrinol 165:217-22. 2000
    ..In addition, our results indicate for the first time that CTBs differentiating along the invasive pathway contain type 1 17beta-HSD mRNA...
  65. ncbi Colony-stimulating factor-1 plays a major role in the development of reproductive function in male mice
    P E Cohen
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Endocrinol 11:1636-50. 1997
    ..However, these males are responsive to GnRH and LH treatment. These studies have identified a novel role for CSF-1 in the development and/or regulation of the male hypothalamic-pituitary-gonadal axis...
  66. ncbi The mouse inhibin alpha-subunit promoter directs SV40 T-antigen to Leydig cells in transgenic mice
    K Kananen
    Department of Physiology, University of Turku, Finland
    Mol Cell Endocrinol 119:135-46. 1996
    ..This gonadal tumor model is valuable for further studies on endocrine functions of Leydig cells and their tumorigenesis in vivo and in vitro...
  67. ncbi Liver receptor homologue-1 is expressed in human steroidogenic tissues and activates transcription of genes encoding steroidogenic enzymes
    R Sirianni
    Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    J Endocrinol 174:R13-7. 2002
    ..Of the promoters examined only HSD3B2 was more sensitive to LRH than SF1. The high level of ovarian and testicular LRH expression make it likely that LRH plays an important role in the regulation of gonadal function...
  68. ncbi Abnormal Leydig cell development at puberty in the androgen-resistant Tfm mouse
    L Murphy
    Department of Veterinary Physiology, University of Glasgow Veterinary School, Scotland
    Endocrinology 135:1372-7. 1994
    ..In addition, 3) the lack of testicular descent at 25 days reduces the pubertal rise in 17 alpha-hydroxylase activity...
  69. ncbi Follicle-stimulating hormone regulates steroidogenic enzymes in cultured cells of the chick embryo ovary
    Y Gomez
    Department of Embryology, Ciudad Universitaria, Mexico City, D F, 04510, Mexico
    Gen Comp Endocrinol 121:305-15. 2001
    ..Somatic cells of the medullary cords are proposed to be FSH target cells in the ovary of the chick embryo...
  70. ncbi Effect of aflatoxins on testicular steroidogenesis and amelioration by vitamin E
    R J Verma
    Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad 380009, India
    Food Chem Toxicol 40:669-72. 2002
    ..The present investigation clearly indicates that vitamin E ameliorates aflatoxin-induced changes in steroidogenesis...
  71. ncbi Ontogeny of expression of the genes for steroidogenic enzymes P450 side-chain cleavage, 3 beta-hydroxysteroid dehydrogenase, P450 17 alpha-hydroxylase/C17-20 lyase, and P450 aromatase in fetal mouse gonads
    T L Greco
    Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor 48109 0278
    Endocrinology 135:262-8. 1994
    ..These data suggest that the absence of fetal ovarian steroid hormone production is the result of lack of expression of at least three of the steroidogenic enzymes, P450scc, P450c17, and P450arom...
  72. ncbi Cholesterol side-chain cleavage cytochrome P450 and 3beta-hydroxysteroid dehydrogenase expression and the concentrations of steroid hormones in the follicular fluids of different phenotypes of healthy and atretic bovine ovarian follicles
    Helen F Irving-Rodgers
    Reproductive Medicine Unit, Department of Obstetrics and Gynaecology, University of Adelaide, Adelaide, South Australia 5005, Australia
    Biol Reprod 69:2022-8. 2003
    ..Second, features of the two forms of follicular atresia are so different as to imply different mechanisms of initiation and regulation...
  73. ncbi The inhibitory effects of manganese on steroidogenesis in rat primary Leydig cells by disrupting steroidogenic acute regulatory (StAR) protein expression
    Jing Cheng
    Department of Toxicology, School of Public Health, Peking University Health Science Center, Xueyuan Road 38, Beijing 100083, People s Republic of China
    Toxicology 187:139-48. 2003
    ..Manganese may also disrupt StAR expression and/or function secondary to mitochondrial dysfunction...
  74. ncbi Onset of steroidogenic enzyme gene expression during ovarian follicular development in sheep
    Kathleen A Logan
    Reproduction Group, AgResearch, Wallaceville Animal Research Centre, Ward Street, Upper Hutt 6007, New Zealand
    Biol Reprod 66:906-16. 2002
    ..These findings raise new questions regarding the roles of steroidogenic regulatory factors in early follicular development...
  75. ncbi 17beta-estradiol treatment decreases steroidogenic enzyme messenger ribonucleic acid levels in the rainbow trout testis
    M Govoroun
    Institut National de la Recherche Agronomique, Laboratoire Institut National de la Recherche Agronomique-Station Commune de Recherches en Ichtyophysiologie, , Campus de Beaulieu, 35042 Rennes Cedex, France
    Endocrinology 142:1841-8. 2001
    ..The decrease in P45011beta mRNA, and thus inhibition of the synthesis of testicular 11-oxygenated androgens, may be an important step required for the active feminization of these genetic males...
  76. ncbi Expression and regulation of fushi tarazu factor-1 and steroidogenic genes during reproduction in Arctic char (Salvelinus alpinus)
    Jonas Von Hofsten
    Department of Molecular Biology, Umea University, Sweden
    Biol Reprod 67:1297-304. 2002
    ..Although these results indicate conserved steroidogenic functions for FTZ-F1 among vertebrates, they also raise the question of additional roles for FTZ-F1 in teleosts...
  77. ncbi Metformin directly inhibits androgen production in human thecal cells
    G R Attia
    Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235-9032, USA
    Fertil Steril 76:517-24. 2001
    ..CONCLUSION(S): Our results suggest that metformin may have a direct effect on thecal cells' androgen production...
  78. ncbi Paracrine-stimulated gene expression profile favors estradiol production in breast tumors
    Sanober A Amin
    Department of Obstetrics and Gynecology, Northwestern University, Chicago, IL 60611, USA
    Mol Cell Endocrinol 253:44-55. 2006
    ....
  79. ncbi Steroid metabolizing enzymes in pluripotential progenitor central nervous system cells: effect of differentiation and maturation
    R C Melcangi
    Department of Endocrinology, University of Milan, Italy
    Neuroscience 72:467-75. 1996
    ..These results show that undifferentiated central nervous system cells possess androgen and progestagen metabolizing enzymes which are strongly influenced by the cellular differentiation/maturation process...
  80. ncbi Sertoli cell proliferations of the infantile testis: an intratubular form of Sertoli cell tumor?
    M Venara
    Endocrinology Division, Children s Hospital Ricardo Gutiérrez, CONICET, Buenos Aires, Argentina
    Am J Surg Pathol 25:1237-44. 2001
    ....
  81. ncbi Changes in the glomerulosa cell phenotype during adrenal regeneration in rats
    W C Engeland
    Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Am J Physiol 276:R1374-82. 1999
    ..These findings show that the enucleation-induced dedifferentitation of the glomerulosa cell may result in part from elevated plasma ACTH and that prevention of dedifferentiation may result in impaired regeneration...
  82. ncbi Morphofunctional changes in Leydig cells throughout the continuous spermatogenesis of the freshwater teleost fish, Serrasalmus spilopleura (Characiformes, Characidae): an ultrastructural and enzyme study
    R H Nóbrega
    Departamento de Biologia Celular, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP, 13083 970, Campinas, SP, Brazil
    Cell Tissue Res 329:339-49. 2007
    ..S. spilopleura thus constitutes an interesting model for increasing our understanding of steroidogenesis control in freshwater teleost fish...
  83. ncbi Inhibition of human cytochrome P450 aromatase activity by butyltins
    D D Heidrich
    Department of Clinical Biochemistry, University of Bonn, Sigmund Freud Strasse 25, D 53125, Bonn, Germany
    Steroids 66:763-9. 2001
    ..Together, these results suggest that the environmental pollutants TBT and DBT, both present in marine organisms, textile and plastic products, may have specific impacts on the metabolism of sex hormones in humans...
  84. ncbi Neurosteroid metabolism in the human brain
    B Stoffel-Wagner
    Department of Clinical Biochemistry, University of Bonn, 53127 Bonn, Germany
    Eur J Endocrinol 145:669-79. 2001
    ....
  85. ncbi A case of 3beta-hydroxysteroid dehydrogenase type II (HSD3B2) deficiency picked up by neonatal screening for 21-hydroxylase deficiency: difficulties and delay in etiologic diagnosis
    Anna Nordenstrom
    Department of Pediatrics and PKU Laboratory, Stockholm, Sweden
    Horm Res 68:204-8. 2007
    ..In addition, elevated 17alpha-hydroxyprogesterone and androstenedione levels due to peripheral HSD3B1 activity may lead to a delay of the correct diagnosis and even to misdiagnosis as CYP21 deficiency...
  86. pmc The developmental increase in adrenocortical 17,20-lyase activity (biochemical adrenarche) is driven primarily by increasing cytochrome b5 in neonatal rhesus macaques
    Ann D Nguyen
    Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, California 95616, USA
    Endocrinology 150:1748-56. 2009
    ..The rhesus macaque is a relevant and accessible model for human ZR development and adrenal function...
  87. ncbi Localization of five steroidogenic enzyme mRNAs in Japanese black bear (Ursus thibetanus japonicus) testes during the mating season by in situ hybridization
    Ruriko Iibuchi
    Laboratory of Wildlife Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
    J Reprod Dev 56:236-42. 2010
    ..We expect that the results of this study will be useful for further investigation of the molecular mechanism of steroidogenic seasonality in the bear testis...
  88. ncbi Some seasonal changes in the thecal gland in the ovaries of hibernators (Citellus citellus L.)
    T Tsvetkov
    Institute for Cryobiology and Lyophilization, Sofia, Bulgaria
    Cryobiology 28:503-8. 1991
    ..A significant increase in the histoenzyme activity of delta 5-3 beta-hydroxysteroid dehydrogenase in December, versus two other enzymes studied, indicated steroid synthesis in theca terna...
  89. ncbi Evaluation of solvent accessibility epitopes for different dehydrogenase inhibitors
    Christian Ludwig
    University of Birmingham, Vincent Drive, Edgbaston, Birmingham B152TT, UK
    ChemMedChem 3:1371-6. 2008
    ..We also evaluate the possibility of using co-solvents, such as DMSO, for magnetization transfer...
  90. ncbi [Purification and some properties of 3 alpha-hydroxysteroid dehydrogenase from pig adrenal cytosol]
    S Nakajin
    Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    Yakugaku Zasshi 111:775-82. 1991
    ..These results strongly suggest that 3 alpha-HSD purified from pig adrenal cytosol is a bi-functional enzyme catalyzing 3 alpha- and 20 alpha-HSD activities...
  91. doi Molecular genetic and bile acid profiles in two Japanese patients with 3beta-hydroxy-DELTA5-C27-steroid dehydrogenase/isomerase deficiency
    Tatsuki Mizuochi
    Department of Pediatrics and Child Health, Division of Gene Therapy and Regenerative Medicine, Kurume University School of Medicine, Kurume, Japan
    Pediatr Res 68:258-63. 2010
    ..Early neonatal diagnosis permits initiation of CDCA treatment at this critical time, before the late cholestatic stage...
  92. pmc Maternal and offspring genetic variants of AKR1C3 and the risk of childhood leukemia
    Chen Yu Liu
    Environmental and Occupational Medicine and Epidemiology Program, Department of Environmental Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA
    Carcinogenesis 29:984-90. 2008
    ..In conclusion, this study suggests that AKR1C3 polymorphisms may be important predictive markers for childhood leukemia susceptibility...
  93. doi Sex- and age-related variation in neurosteroidogenic enzyme mRNA levels during quail embryonic development
    Nicoletta Aste
    Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464 8601 Japan
    Brain Res 1201:15-22. 2008
    ..These results, taken together, are suggestive of a potential role of some neurosteroidogenic enzymes in the development of quail brain and suggest that sexual differences in the hormonal environment may occur during brain development...
  94. ncbi Hypophyseal corticosteroids stimulate somatotrope differentiation in the embryonic chicken pituitary gland
    Jun Zheng
    Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama, Japan
    Histochem Cell Biol 129:357-65. 2008
    ....
  95. pmc Nonreplication of the type 5 17beta-hydroxysteroid dehydrogenase gene association with polycystic ovary syndrome
    Mark O Goodarzi
    Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Cedars Sinai Medical Center, Los Angeles, California 90048, USA
    J Clin Endocrinol Metab 93:300-3. 2008
    ....
  96. ncbi [Clinical features and diagnosis of mild 3-beta-hydroxysteroid dehydrogenase deficiency in men]
    K Frank Raue
    , , Heidelberg
    Dtsch Med Wochenschr 114:331-4. 1989
    ..The prevalence of late-onset HSD deficiency in men is not known and may be more relevant in patients with gynaecomastia or abnormal gonadal function than has hitherto been realized...
  97. ncbi The murine 3 beta-hydroxysteroid dehydrogenase multigene family: structure, function and tissue-specific expression
    A H Payne
    Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor 48109 0278, USA
    J Steroid Biochem Mol Biol 53:111-8. 1995
    ..The four structural genes encoding the different isoforms are closely linked within a segment of mouse chromosome 3 that is conserved on human chromosome 1...
  98. ncbi Distribution of 3 alpha-hydroxysteroid dehydrogenase in rat brain and molecular cloning of multiple cDNAs encoding structurally related proteins in humans
    M Khanna
    Department of Pediatrics, Cornell University Medical College, New York, NY 10021, USA
    J Steroid Biochem Mol Biol 53:41-6. 1995
    ..We are currently investigating the functional relationship between the enzymes encoded by these human cDNAs and 3 alpha-hydroxysteroid dehydrogenase...
  99. ncbi Functional characterization of the novel L108W and P186L mutations detected in the type II 3 beta-hydroxysteroid dehydrogenase gene of a male pseudohermaphrodite with congenital adrenal hyperplasia
    R Sanchez
    Medical Research Council Group in Molecular Endocrinology, CHUL Research Center, Quebec, Canada
    Hum Mol Genet 3:1639-45. 1994
    ..3% and 0.2% for the L108W and P186L enzymes, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)..
  100. ncbi Melatonin in the regulation of annual testicular events in carp Catla catla: evidence from the studies on the effects of exogenous melatonin, continuous light, and continuous darkness
    Sharmistha Bhattacharya
    Department of Zoology, Visva Bharati University, Santiniketan 731 235, India
    Chronobiol Int 24:629-50. 2007
    ....
  101. doi Evidence of limited contributions for intratumoral steroidogenesis in prostate cancer
    Johannes Hofland
    Departments of Internal Medicine, Urology, and Pathology, Erasmus MC, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Cancer Res 70:1256-64. 2010
    ....

Research Grants75

  1. Structure/Function of Steroid Transforming AKR's
    Trevor M Penning; Fiscal Year: 2010
    ..This application will establish whether these genetic changes result in abnormal bile-acid production and are causal in these deficiencies. ..
  2. Molecular Mechanisms of Multistage Carcinogenesis
    TREVOR PENNING; Fiscal Year: 2006
    ..By studying the discrete molecular events responsible for the causation of cancer this Program may lead to the early prevention and intervention of this disease. ..
  3. Structure/Function of Steroid Hormone Transforming AKRs
    TREVOR PENNING; Fiscal Year: 2006
    ..Completion of these aims will provide structural details of how AKRs transform steroid hormones. ..
  4. Aldo-Keto Reductases and PAH Metabolism/Activation
    TREVOR PENNING; Fiscal Year: 2006
    ..Validation of a role for AKRs in PAH-induced lung carcinogenesis may target this pathway for prevention and intervention of this disease. ..
  5. Human Aldo Keto Reductases and Steroid Hormone Action
    TREVOR PENNING; Fiscal Year: 2005
    ..These inhibitors may provide tools to dissect function and provide routes to the first Selective Intracrine Modulators that target AKRs. ..
  6. Structure/Function of Steroid Hormone Transforming AKRs
    TREVOR PENNING; Fiscal Year: 2004
    ..Completion of these aims will provide structural details of how AKRs transform steroid hormones. ..
  7. Human Aldo Keto Reductases and Steroid Hormone Action
    TREVOR PENNING; Fiscal Year: 2004
    ..These inhibitors may provide tools to dissect function and provide routes to the first Selective Intracrine Modulators that target AKRs. ..
  8. Human Aldo Keto Reductases and Steroid Hormone Action
    TREVOR PENNING; Fiscal Year: 2003
    ..These inhibitors may provide tools to dissect function and provide routes to the first Selective Intracrine Modulators that target AKRs. ..
  9. Structure/Function of Steroid Hormone Transforming AKRs
    TREVOR PENNING; Fiscal Year: 2003
    ..Completion of these aims will provide structural details of how AKRs transform steroid hormones. ..
  10. Aldo-Keto Reductases and PAH Metabolism/Activation
    TREVOR PENNING; Fiscal Year: 2003
    ..Validation of a role for AKRs in PAH-induced lung carcinogenesis may target this pathway for prevention and intervention of this disease. ..
  11. Symposium on Aldo-Keto Reductases & Toxicant Metabolism
    TREVOR PENNING; Fiscal Year: 2002
    ..abstract_text> ..
  12. STRUCTURE/FUNCTION OF 3A-HYDROXYSTEROID DEHYDROGENASE
    TREVOR PENNING; Fiscal Year: 2002
    ....
  13. Human Aldo Keto Reductases and Steroid Hormone Action
    TREVOR PENNING; Fiscal Year: 2002
    ..These inhibitors may provide tools to dissect function and provide routes to the first Selective Intracrine Modulators that target AKRs. ..
  14. Aldo-Keto Reductases and PAH Metabolism/Activation
    TREVOR PENNING; Fiscal Year: 2007
    ..Validation of a role for AKRs in PAH-induced lung carcinogenesis may target this pathway for prevention and intervention of this disease. ..
  15. Pathways of PAH activation in human lung cells
    TREVOR PENNING; Fiscal Year: 2007
    ..These studies will identify major BP-metabolites in NHBE cells which could be used to biomonitor human PAH exposure, and validate candidate genes for genetic predisposition studies. ..
  16. Human aldo-keto reductases and nuclear receptor action
    Trevor M Penning; Fiscal Year: 2010
    ..Aim 4, will use crystal structures of AKR1C3"NADP(H)"NSAID complexes to develop AKR1C3 isoform specific inhibitors. These studies will establish whether AKR1C3 is a non-Cox target for the cancer chemopreventive roles of NSAIDs. ..
  17. Human aldo-keto reductases and nuclear receptor action
    TREVOR PENNING; Fiscal Year: 2009
    ..This proposal is aimed at developing a stable isotope dilution liquid chromatography mass spectrometry method to measure the intraprostatic androgen metabolome which could be used to better diagnose and treat prostate cancer. ..
  18. Pathways of PAH activation in human lung cells
    Trevor M Penning; Fiscal Year: 2010
    ..These studies will identify major BP-metabolites in NHBE cells which could be used to biomonitor human PAH exposure, and validate candidate genes for genetic predisposition studies. ..
  19. Aldo-Keto Reductases and PAH Metabolism/Activation
    Trevor M Penning; Fiscal Year: 2010
    ..These studies will inform genotyping and validate genomic studies of cancer of the airway and improve risk assessment prediction. ..
  20. Structure/Function of Steroid Transforming AKR's
    TREVOR PENNING; Fiscal Year: 2009
    ..This application will establish whether these genetic changes result in abnormal bile-acid production and are causal in these deficiencies. ..
  21. Aldo-Keto Reductases and PAH Metabolism/Activation
    TREVOR PENNING; Fiscal Year: 2009
    ..These studies will inform genotyping and validate genomic studies of cancer of the airway and improve risk assessment prediction. ..
  22. Pathways of PAH activation in human lung cells
    TREVOR PENNING; Fiscal Year: 2009
    ..These studies will identify major BP-metabolites in NHBE cells which could be used to biomonitor human PAH exposure, and validate candidate genes for genetic predisposition studies. ..
  23. Human aldo-keto reductases and nuclear receptor action
    TREVOR PENNING; Fiscal Year: 2009
    ..Aim 4, will use crystal structures of AKR1C3"NADP(H)"NSAID complexes to develop AKR1C3 isoform specific inhibitors. These studies will establish whether AKR1C3 is a non-Cox target for the cancer chemopreventive roles of NSAIDs. ..
  24. Structure/Function of Steroid Transforming AKR's
    TREVOR PENNING; Fiscal Year: 2008
    ..This application will establish whether these genetic changes result in abnormal bile-acid production and are causal in these deficiencies. ..
  25. Pathways of PAH activation in human lung cells
    TREVOR PENNING; Fiscal Year: 2008
    ..These studies will identify major BP-metabolites in NHBE cells which could be used to biomonitor human PAH exposure, and validate candidate genes for genetic predisposition studies. ..
  26. Human aldo-keto reductases and nuclear receptor action
    TREVOR PENNING; Fiscal Year: 2008
    ..Aim 4, will use crystal structures of AKR1C3"NADP(H)"NSAID complexes to develop AKR1C3 isoform specific inhibitors. These studies will establish whether AKR1C3 is a non-Cox target for the cancer chemopreventive roles of NSAIDs. ..
  27. Center of Excellence in Environmental Toxicology
    TREVOR PENNING; Fiscal Year: 2008
    ..The COEP has forged relationships with community partners for outreach. The COEP goal will be to reduce the risk of harmful exposures and improve the public health of vulnerable populations within these communities. ..
  28. Structure/Function of Steroid Transforming AKR's
    TREVOR PENNING; Fiscal Year: 2007
    ..This application will establish whether these genetic changes result in abnormal bile-acid production and are causal in these deficiencies. ..
  29. Human aldo-keto reductases and nuclear receptor action
    TREVOR PENNING; Fiscal Year: 2007
    ..Aim 4, will use crystal structures of AKR1C3"NADP(H)"NSAID complexes to develop AKR1C3 isoform specific inhibitors. These studies will establish whether AKR1C3 is a non-Cox target for the cancer chemopreventive roles of NSAIDs. ..
  30. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 2002
    ..Sites of cleavage or base modification will be compared to mutational hot spots observed in human tumors. The transforming potential of BPQ treated ras will be measured by foci formation in NIH/3T3 cells. ..
  31. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM
    TREVOR PENNING; Fiscal Year: 1990
    ..The mRNA species coding for the human enzyme will be identified by Northern analysis using the cDNA for the rat liver enzyme...
  32. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 1993
    ..The mutagenicity of these PAH o-quinones will be assessed using Salmonella tester strains (Ta102 and TA104) which are sensitive to mutagens that cause oxidative DNA damage...
  33. STRUCTURE/FUNCTION OF 3A-HYDROXYSTEROID DEHYDROGENASE
    TREVOR PENNING; Fiscal Year: 1999
    ....
  34. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 1999
    ..Sites of cleavage or base modification will be compared to mutational hot spots observed in human tumors. The transforming potential of BPQ treated ras will be measured by foci formation in NIH/3T3 cells. ..
  35. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM
    TREVOR PENNING; Fiscal Year: 1991
    ..The mRNA species coding for the human enzyme will be identified by Northern analysis using the cDNA for the rat liver enzyme...
  36. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 2000
    ..Sites of cleavage or base modification will be compared to mutational hot spots observed in human tumors. The transforming potential of BPQ treated ras will be measured by foci formation in NIH/3T3 cells. ..
  37. Structure/Function of Steroid Hormone Transforming AKRs
    TREVOR PENNING; Fiscal Year: 2005
    ..Completion of these aims will provide structural details of how AKRs transform steroid hormones. ..
  38. Human Aldo Keto Reductases and Steroid Hormone Action
    TREVOR PENNING; Fiscal Year: 2001
    ..These inhibitors may provide tools to dissect function and provide routes to the first Selective Intracrine Modulators that target AKRs. ..
  39. STRUCTURE/FUNCTION OF 3A-HYDROXYSTEROID DEHYDROGENASE
    TREVOR PENNING; Fiscal Year: 2001
    ....
  40. STRUCTURE/FUNCTION OF 3A-HYDROXYSTEROID DEHYDROGENASE
    TREVOR PENNING; Fiscal Year: 2000
    ....
  41. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 2000
    ..Sites of cleavage or base modification will be compared to mutational hot spots observed in human tumors. The transforming potential of BPQ treated ras will be measured by foci formation in NIH/3T3 cells. ..
  42. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 2001
    ..Sites of cleavage or base modification will be compared to mutational hot spots observed in human tumors. The transforming potential of BPQ treated ras will be measured by foci formation in NIH/3T3 cells. ..
  43. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM
    TREVOR PENNING; Fiscal Year: 1992
    ..The mRNA species coding for the human enzyme will be identified by Northern analysis using the cDNA for the rat liver enzyme...
  44. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 2001
    ..Sites of cleavage or base modification will be compared to mutational hot spots observed in human tumors. The transforming potential of BPQ treated ras will be measured by foci formation in NIH/3T3 cells. ..
  45. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 1999
    ..Sites of cleavage or base modification will be compared to mutational hot spots observed in human tumors. The transforming potential of BPQ treated ras will be measured by foci formation in NIH/3T3 cells. ..
  46. PLACENTAL 3 BETA-HYDROXYSTEROID DEHYDROGENASE/ISOMERASE
    James Thomas; Fiscal Year: 2001
    ..The absence of detergent in the pure cytosolic enzyme preparation facilitates the growth of crystals for future structural studies. ..
  47. Placental 3B-Hydroxysteroid Dehydrogenase/Isomerase
    James Thomas; Fiscal Year: 2007
    ..These studies may ultimately produce new treatments for the prevention of premature birth and the treatment of hormone-sensitive breast cancer while leaving adrenal steroidogenesis intact. [unreadable] [unreadable]..
  48. PLACENTAL 3 BETA-HYDROXYSTEROID DEHYDROGENASE/ISOMERASE
    James Thomas; Fiscal Year: 2003
    ..The absence of detergent in the pure cytosolic enzyme preparation facilitates the growth of crystals for future structural studies. ..
  49. Placental 3B-Hydroxysteroid Dehydrogenase/Isomerase
    James Thomas; Fiscal Year: 2008
    ..These studies may ultimately produce new treatments for the prevention of premature birth and the treatment of hormone-sensitive breast cancer while leaving adrenal steroidogenesis intact. [unreadable] [unreadable]..
  50. Placental 3B-Hydroxysteroid Dehydrogenase/Isomerase
    James Thomas; Fiscal Year: 2006
    ..These studies may ultimately produce new treatments for the prevention of premature birth and the treatment of hormone-sensitive breast cancer while leaving adrenal steroidogenesis intact. [unreadable] [unreadable]..
  51. PLACENTAL 3 BETA-HYDROXYSTEROID DEHYDROGENASE/ISOMERASE
    James Thomas; Fiscal Year: 2000
    ..The absence of detergent in the pure cytosolic enzyme preparation facilitates the growth of crystals for future structural studies. ..
  52. PLACENTAL 3 BETA-HYDROXYSTEROID DEHYDROGENASE/ISOMERASE
    James Thomas; Fiscal Year: 2002
    ..The absence of detergent in the pure cytosolic enzyme preparation facilitates the growth of crystals for future structural studies. ..
  53. NEURAL STEROIDOGENIC ENZYMES AND BRAIN FUNCTION
    BARNETT SCHLINGER; Fiscal Year: 2001
    ..We believe these studies will contribute significantly to our understanding of the steroidogenic capability of the brain, and expand concepts of steroids as neural signaling molecules. ..
  54. Pharmacogenomics and Mechanisms of Cytidine Analogues
    Liewei Wang; Fiscal Year: 2009
    ....
  55. Physiology of Hypothalamic Neurosteroidal Progesterone
    Paul Micevych; Fiscal Year: 2006
    ..These studies will demonstrate the physiology of neurosteroidal progesterone and provide important new information about the mechanism of estrogen-positive feedback in the CNS. ..
  56. SEX STEROID ACTIVATION OF OPIOID CIRCUITS IN THE CNS
    Paul Micevych; Fiscal Year: 2004
    ..Such experiments will functionally define the interaction of steroids and opioids modulating lordosis, and demonstrate a category of analyses that compare patterns of receptor activation and behavioral output of specific circuits. ..
  57. NEURAL STEROIDOGENIC ENZYMES AND BRAIN FUNCTION
    BARNETT SCHLINGER; Fiscal Year: 2000
    ..We believe these studies will contribute significantly to our understanding of the steroidogenic capability of the brain, and expand concepts of steroids as neural signaling molecules. ..
  58. NEURAL STEROIDOGENIC ENZYMES AND BRAIN FUNCTION
    BARNETT SCHLINGER; Fiscal Year: 2005
    ..abstract_text> ..
  59. Activation of androgen biosynthesis and drug metabolism by cytochrome b5
    Richard Auchus; Fiscal Year: 2009
    ..This knowledge will provide a novel approach for suppressing androgen production, by targeting the CYP17A1-b5 interaction. ..