Summary: A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).
Publications289 found, 100 shown here
- Molecular analysis of herbivore-induced condensed tannin synthesis: cloning and expression of dihydroflavonol reductase from trembling aspen (Populus tremuloides)Darren J Peters
Department of Biological Sciences, University of Alberta, Edmonton, Canada T6G 2E9
Plant J 32:701-12. 2002..Our findings suggest that the induction of condensed tannins, compounds known to be important for defense against herbivores, is mediated by increased expression of DFR and other phenylpropanoid genes...
- Transcriptional regulation of BRCA1 expression by a metabolic switchLi Jun Di
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, Maryland, USA
Nat Struct Mol Biol 17:1406-13. 2010..The active control of chromatin marks, DNA accessibility and gene expression at the BRCA1 promoter by this 'metabolic switch' provides an important molecular link between caloric intake and tumor suppressor expression in mammary cells...
- CtBP, an unconventional transcriptional corepressor in development and oncogenesisG Chinnadurai
Institute for Molecular Virology, Saint Louis University School of Medicine, 3681 Park Avenue, St Louis, MO 63110, USA
Mol Cell 9:213-24. 2002..CtBPs play important roles during development and oncogenesis. In this review, their unusual properties, the mechanisms of transcriptional repression, regulation, and their biological functions are discussed...
- Silencing of NADPH-dependent oxidoreductase genes (yqhD and dkgA) in furfural-resistant ethanologenic Escherichia coliE N Miller
Department of Microbiology and Cell Science, University of Florida, Gainesville, 32611, USA
Appl Environ Microbiol 75:4315-23. 2009..Together, these results suggest that the process of reducing furfural by using an enzyme with a low K(m) for NADPH rather than a direct inhibitory action is the primary cause for growth inhibition by low concentrations of furfural...
- Aldo-keto reductases and bioactivation/detoxicationYi Jin
Department of Pharmacology, Center of Excellence in Environmental Toxicology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Annu Rev Pharmacol Toxicol 47:263-92. 2007..g., aflatoxin, endogenous toxicants, and those formed from the breakdown of lipid peroxides. AKRs are stress-regulated genes and play a central role in the cellular response to osmotic, electrophilic, and oxidative stress...
- Homeodomain-interacting protein kinase-2 mediates CtBP phosphorylation and degradation in UV-triggered apoptosisQinghong Zhang
Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
Proc Natl Acad Sci U S A 102:2802-7. 2005..Consequently, interference with HIPK2 action in H1299 cells rescued UV-triggered apoptosis...
- Downregulation of cinnamyl-alcohol dehydrogenase in switchgrass by RNA silencing results in enhanced glucose release after cellulase treatmentAaron J Saathoff
Grain, Forage, and Bioenergy Research Unit, United States Department of Agriculture Agricultural Research Service, Lincoln, Nebraska, United States of America
PLoS ONE 6:e16416. 2011..When stained with the lignin and cutin specific stain phloroglucinol-HCl the staining intensity of one line indicated greater incorporation of hydroxycinnamyl aldehydes in the lignin...
- Characterization of a Saccharomyces cerevisiae NADP(H)-dependent alcohol dehydrogenase (ADHVII), a member of the cinnamyl alcohol dehydrogenase familyCarol Larroy
Department of Biochemistry and Molecular Biology, Universitat Autonoma de Barcelona, Barcelona, Spain
Eur J Biochem 269:5738-45. 2002..Simultaneous deletion of ADH6 and ADH7 was not lethal for the yeast. Both enzymes could participate in the synthesis of fusel alcohols, ligninolysis and NADP(H) homeostasis...
- Homeodomain interacting protein kinase 2 promotes apoptosis by downregulating the transcriptional corepressor CtBPQinghong Zhang
Vollum Institute, Oregon Health and Science University, 3181 S W Sam Jackson Park Road, Portland, OR 97239, USA
Cell 115:177-86. 2003..Finally, reduction in CtBP levels, either by genetic knockout or siRNA, promoted apoptosis in p53-deficient cells. These findings provide a pathway for UV-induced apoptosis in cells lacking p53...
- A two-step model for colon adenoma initiation and progression caused by APC lossReid A Phelps
Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA
Cell 137:623-34. 2009..Consistent with this model, human FAP adenomas showed robust upregulation of CtBP1 in the absence of detectable nuclear beta-catenin, whereas nuclear beta-catenin was detected in carcinomas...
- The Bacillus subtilis ydjL (bdhA) gene encodes acetoin reductase/2,3-butanediol dehydrogenaseWayne L Nicholson
Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899, USA
Appl Environ Microbiol 74:6832-8. 2008..From the data, it is proposed that the major AR/BDH-encoding gene ydjL be renamed bdhA...
- A genomewide analysis of the cinnamyl alcohol dehydrogenase family in sorghum [Sorghum bicolor (L.) Moench] identifies SbCAD2 as the brown midrib6 geneAna Saballos
School of Molecular Biosciences, Washington State University, Pullman, Washington 99164, USA
Genetics 181:783-95. 2009..The modeling revealed unique changes in structure consistent with the observed phenotypes of the mutants...
- The quinoprotein dehydrogenases for methanol and glucoseChristopher Anthony
School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
Arch Biochem Biophys 428:2-9. 2004..By contrast, mGDH contains a permanently bound ubiquinone, which acts as a single electron carrier, mediating electron transfer through the protein to the membrane ubiquinone...
- Cloning and overexpression of the 3-hydroxyisobutyrate dehydrogenase gene from pseudomonas putida E23Emran Kabir Chowdhury
Department of Bioresources Science, Kochi University, Nankoku, Kochi 783 8502, Japan
Biosci Biotechnol Biochem 67:438-41. 2003..Lysine and asparagine residues, which are important in catalysis of the 3-hydroxyacid dehydrogenase family, are conserved in this enzyme...
- The oligomeric state of CtBP determines its role as a transcriptional co-activator and co-repressor of Wingless targetsChandan Bhambhani
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA
EMBO J 30:2031-43. 2011..Functional interactions between CtBP and Pygopus, a nuclear protein required for Wingless signalling, support a model where monomeric CtBP acts downstream of Pygopus in activating some Wingless targets...
- Redox-dependent Brca1 transcriptional regulation by an NADH-sensor CtBP1Y Deng
Department of Dermatology, University of Colorado, Denver, Aurora, CO 80045, USA
Oncogene 29:6603-8. 2010....
- Evolution of the Cinnamyl/Sinapyl Alcohol Dehydrogenase (CAD/SAD) gene family: the emergence of real lignin is associated with the origin of Bona Fide CADDong mei Guo
State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, The Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Beijing, 100093, China
J Mol Evol 71:202-18. 2010..Our study also supports the hypothesis of convergent evolution of lignin biosynthesis between red algae and vascular plants...
- Genome-wide characterization of the lignification toolbox in ArabidopsisJeroen Raes
Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, Technologiepark 927, B 9052 Gent, Belgium
Plant Physiol 133:1051-71. 2003..Together, these data describe the full complement of monolignol biosynthesis genes in Arabidopsis, provide a unified nomenclature, and serve as a basis for further functional studies...
- Molecular and biochemical analysis of two cDNA clones encoding dihydroflavonol-4-reductase from Medicago truncatulaDe Yu Xie
Plant Biology Division, The Samuel Roberts Noble Foundation, Inc, 2510 Sam Noble Parkway, Ardmore, Oklahoma 73402, USA
Plant Physiol 134:979-94. 2004..The data reveal unexpected properties and differences in two DFR proteins from a single species...
- p19Arf inhibits the invasion of hepatocellular carcinoma cells by binding to C-terminal binding proteinYa Wen Chen
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Cancer Res 68:476-82. 2008..Thus, our data indicate a novel role for the Arf tumor suppressor protein in regulating phenotypes associated with tumor progression and metastasis in HCC cells...
- Net, a negative Ras-switchable TCF, contains a second inhibition domain, the CID, that mediates repression through interactions with CtBP and de-acetylationP Criqui-Filipe
Institut de Genetique et de Biologie Moleculaire et Cellulaire, ECNRS INSERM ULP, 1 rue Laurent Fries, BP 163, 67404 Illkirch Cedex, France
EMBO J 18:3392-403. 1999..These results provide insights into the cross-talk between pathways that inhibit and stimulate transformation at the level of Net, a regulator of gene expression...
- Cinnamyl alcohol dehydrogenases-C and D, key enzymes in lignin biosynthesis, play an essential role in disease resistance in ArabidopsisMaurice Tronchet
Laboratoire des Interactions Plantes Microorganismes LIPM, UMR CNRS INRA 2594 441, BP 52627, F 31326 Castanet Tolosan Cedex, France
Mol Plant Pathol 11:83-92. 2010..Thus, in contrast with cellulose synthesis, whose alteration leads to an increase in disease resistance, alteration of the cell wall lignin content leads directly or indirectly to defects in some defence components...
- Identification of genes required for Pseudomonas aeruginosa carnitine catabolismMatthew J Wargo
The Vermont Lung Center, Department of Medicine, University of Vermont College of Medicine, Burlington, VT 05405, USA
Microbiology 155:2411-9. 2009..These results provide important insight into elements required for carnitine catabolism in P. aeruginosa and probably in other bacteria...
- Nuclear speckle-associated protein Pnn/DRS binds to the transcriptional corepressor CtBP and relieves CtBP-mediated repression of the E-cadherin geneRoman Alpatov
Department of Anatomy and Cell Biology, 1600 SW Archer Rd, University of Florida College of Medicine, Gainesville, FL 32610 0235, USA
Mol Cell Biol 24:10223-35. 2004..This interaction may reflect the existence of coupling factors involved in CtBP-mediated transcriptional regulation and mRNA processing events...
- Molecular cloning and characterization of two genes encoding dihydroflavonol-4-reductase from Populus trichocarpaYan Huang
Key Laboratory of Eco environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, China
PLoS ONE 7:e30364. 2012....
- Phylogeny and structure of the cinnamyl alcohol dehydrogenase gene family in Brachypodium distachyonChristian Bukh
Department of Plant and Environmental Sciences, University of Copenhagen, DK 1871 Frederiksberg C, Denmark
J Exp Bot 63:6223-36. 2012..All CAD enzymes are cytosolic except for BdCAD4, which has a putative chloroplast signal peptide adding to the diversity of CAD functions...
- Modification of flavonoid biosynthesis in crop plantsElio G W M Schijlen
Plant Research International, Business Unit Bioscience, P O Box 16, 6700 AA Wageningen, The Netherlands
Phytochemistry 65:2631-48. 2004..This review describes the current knowledge of the molecular regulation of the flavonoid pathway and the state of the art with respect to metabolic engineering of this pathway in crop plants...
- Aldo-keto reductase (AKR) superfamily: genomics and annotationRebekka D Mindnich
Department of Pharmacology, Center for Excellence in Environmental Toxicology, University of Pennsylvania, School of Medicine, Philadelphia, PA 19104 6084, USA
Hum Genomics 3:362-70. 2009..Recent developments in the annotation of SNP and transcript variance in AKRs are also summarised...
- OsCAD2 is the major CAD gene responsible for monolignol biosynthesis in rice culmKo Hirano
Bioscience and Biotechnology Center, Nagoya University, Chikusa ku, Nagoya, Aichi, Japan
Plant Cell Rep 31:91-101. 2012..These results suggest that OsCAD2 is largely responsible for monolignol biosynthesis in rice, which is similar to that indicated for the predominant role of other plant bona fide CAD protein to monolignol biosynthesis...
- Autophagy induction by tetrahydrobiopterin deficiencySang Su Kwak
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea
Autophagy 7:1323-34. 2011..In addition, this study first documents mTORC1 inactivation and autophagy induction in PKU patients with BH₄ deficiency...
- Disrupting the cinnamyl alcohol dehydrogenase 1 gene (BdCAD1) leads to altered lignification and improved saccharification in Brachypodium distachyonMadeleine Bouvier d'Yvoire
Institut Jean Pierre Bourgin, Unité Mixte de Recherche 1318 INRA AgroParisTech, Institut National de la Recherche Agronomique Centre de Versailles Grignon, Route de St Cyr RD10, 78026, Versailles, France
Plant J 73:496-508. 2013..These changes make saccharification of cells walls pre-treated with alkaline easier without compromising plant growth...
- Microbial conversion of glycerol to 1,3-propanediol by an engineered strain of Escherichia coliXueming Tang
Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai 201106, People s Republic of China
Appl Environ Microbiol 75:1628-34. 2009..To our knowledge, this is the highest reported yield and productivity efficiency of 1,3-PD with glycerol as the sole source of carbon. Furthermore, the overall fermentation time was only 40 h, shorter than that of any other reports...
- Differential spatial expression and subcellular localization of CtBP family members in rodent brainDiana Hübler
Department of Neurochemistry and Molecular Biology, Leibniz Institute for Neurobiology, Magdeburg, Germany
PLoS ONE 7:e39710. 2012..Finally, we propose a mechanism of differential synapto-nuclear targeting of its splice variants CtBP2-S and CtBP2-L in neurons...
- GMC oxidoreductases. A newly defined family of homologous proteins with diverse catalytic activitiesD R Cavener
Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235
J Mol Biol 223:811-4. 1992..These enzymes contain a canonical ADP-binding beta alpha beta-fold close to their amino termini as found in other flavoenzymes. This domain is encoded by a single exon of the D. melanogaster glucose dehydrogenase gene...
- Structural and functional analysis of A-type ketoreductases from the amphotericin modular polyketide synthaseJianting Zheng
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX 78712 0165, USA
Structure 18:913-22. 2010..Together, these results highlight the possibility of region-specific modification of polyketides through active site engineering of KRs...
- Properties of short-chain dehydrogenase/reductase RalR1: characterization of purified enzyme, its orientation in the microsomal membrane, and distribution in human tissues and cell linesOlga V Belyaeva
Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri Kansas City, 64110, USA
Biochemistry 42:14838-45. 2003..The expression pattern and the high catalytic efficiency of RalR1 are consistent with the hypothesis that RalR1 contributes to the reduction of retinal in various human tissues...
- A high association with cone dystrophy in Fundus albipunctatus caused by mutations of the RDH5 geneM Nakamura
Department of Ophthalmology, Nagoya University School of Medicine, Nagoya, Japan
Invest Ophthalmol Vis Sci 41:3925-32. 2000....
- Fundus albipunctatus associated with compound heterozygous mutations in RPE65Patrik Schatz
Department of Ophthalmology, Glostrup Hospital, University of Copenhagen, Copenhagen, Denmark
Ophthalmology 118:888-94. 2011..To describe a family with an 18-year-old woman with fundus albipunctatus and compound heterozygous mutations in RPE65 whose unaffected parents and 1 female sibling harbored single heterozygous RPE65 mutations...
- A novel Gly35Ser mutation in the RDH5 gene in a Japanese family with fundus albipunctatus associated with cone dystrophyY Wada
Department of Ophthalmology, Tohoku University School of Medicine, 1 1 Seiryo machi, Aoba ku, Sendai 980 77, Japan
Arch Ophthalmol 119:1059-63. 2001..To assess the clinical and genetic characteristics of a Japanese family with fundus albipunctatus with progressive cone dystrophy associated with a mutation in the RDH5 gene...
- Characterization of the Saccharomyces cerevisiae YMR318C (ADH6) gene product as a broad specificity NADPH-dependent alcohol dehydrogenase: relevance in aldehyde reductionCarol Larroy
Department of Biochemistry and Molecular Biology, Faculty of Sciences, Universitat Autonoma de Barcelona, E 08193 Bellaterra Barcelona, Spain
Biochem J 361:163-72. 2002..The enzyme may also be involved in the synthesis of fusel alcohols. To our knowledge this is the first NADPH-dependent medium-chain ADH to be characterized in S. cerevisiae...
- A nonsense mutation in a cinnamyl alcohol dehydrogenase gene is responsible for the Sorghum brown midrib6 phenotypeScott E Sattler
Grain, Forage, and Bioenergy Research Unit, United States Department of Agriculture Agricultural Research Service, Nebraska, USA
Plant Physiol 150:584-95. 2009..These differences include His-57, which is unique to Bmr6 and other grass CADs. In summary, Bmr6 encodes the major CAD protein involved in lignin synthesis in sorghum, and the bmr6 mutant is a null allele...
- Down-regulation of cinnamyl alcohol dehydrogenase in transgenic alfalfa (Medicago sativa L.) and the effect on lignin composition and digestibilityM Baucher
Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, Belgium
Plant Mol Biol 39:437-47. 1999..This study opens perspectives for improving forage crop digestibility by the modulation of enzymes involved in lignin biosynthesis...
- Physical and functional interactions between the corepressor CtBP and the Epstein-Barr virus nuclear antigen EBNA3CR Touitou
Section of Virology and Cell Biology and Ludwig Institute for Cancer Research, Imperial College of Science, Technology and Medicine, St Mary s Campus, London W2 1PG, United Kingdom
J Virol 75:7749-55. 2001..A striking correlation between CtBP binding and the capacity of EBNA3C to cooperate with (Ha-)Ras in the immortalization and transformation of primary rat embryo fibroblasts was also revealed...
- High glycolate oxidase activity is required for survival of maize in normal airIsrael Zelitch
Department of Biochemistry and Genetics, The Connecticut Agricultural Experiment Station, New Haven, Connecticut 06511, USA
Plant Physiol 149:195-204. 2009..These studies show that C4 photosynthesis in maize is dependent on photorespiration throughout seedling development and support the view that the carbon oxidation pathway evolved to prevent accumulation of toxic glycolate...
- Medium- and short-chain dehydrogenase/reductase gene and protein families : Medium-chain and short-chain dehydrogenases/reductases in retinoid metabolismX Pares
Department of Biochemistry and Molecular Biology, Universitat Autonoma de Barcelona, Bellaterra, Spain
Cell Mol Life Sci 65:3936-49. 2008..The large number of enzymes involved in the two directions of this step, also including aldo-keto reductases, suggests that retinaldehyde levels are strictly regulated...
- Engineering of 2,3-butanediol dehydrogenase to reduce acetoin formation by glycerol-overproducing, low-alcohol Saccharomyces cerevisiaeMaryam Ehsani
INRA, UMR 1083, F 34060 Montpellier, France
Appl Environ Microbiol 75:3196-205. 2009....
- Flavonoid biosynthesis in the tea plant Camellia sinensis: properties of enzymes of the prominent epicatechin and catechin pathwaysP A N Punyasiri
Biochemistry Division, Tea Research Institute of Sri Lanka, Talawakelle, Sri Lanka
Arch Biochem Biophys 431:22-30. 2004..Together with the enzyme activities and substrate specificities of the preceding enzymatic reactions, the biosynthesis of the most prominent flavonoids of tea is elucidated...
- Phosphorylation of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein reductase MabA regulates mycolic acid biosynthesisRomain Veyron-Churlet
Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologiques, Universite de Montpellier I, CNRS, UMR 5235, Case 107, Place Eugene Bataillon, 34095 Montpellier Cedex 05, France
J Biol Chem 285:12714-25. 2010..This study provides the first information on the molecular mechanism(s) involved in mycolic acid regulation through Ser/Thr protein kinase-dependent phosphorylation of a type II fatty acid synthase enzyme...
- The C-terminal (BRCT) domains of BRCA1 interact in vivo with CtIP, a protein implicated in the CtBP pathway of transcriptional repressionX Yu
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA
J Biol Chem 273:25388-92. 1998..These results indicate that the BRCA1-CtIP interaction may be required for tumor suppression by BRCA1...
- Sequence and expression levels in human tissues of a new member of the aldo-keto reductase familyD J Hyndman
Department of Biochemistry, Queen s University, Kingston, Canada
Biochim Biophys Acta 1399:198-202. 1998..It is also strongly expressed in the intestinal tract and has been called human small intestine reductase...
- Fluorescence imaging-based screen identifies ARF GEF component of early endosomal traffickingHirokazu Tanaka
Department of Plant Systems Biology, Flanders Institute for Biotechnology VIB and Department of Molecular Genetics, Ghent University, 9052 Gent, Belgium
Curr Biol 19:391-7. 2009..BEN1 is involved in endocytosis of plasma membrane proteins and localizes to early endocytic compartments distinct from GNOM-positive endosomes. Our results identify BEN1 as the ARF GEF mediating early endosomal traffic...
- Drosophila CtBP: a Hairy-interacting protein required for embryonic segmentation and hairy-mediated transcriptional repressionG Poortinga
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA
EMBO J 17:2067-78. 1998..While Hairy is probably not the only segmentation gene interacting with dCtBP, we show dose-sensitive genetic interactions between dCtBP and hairy mutations...
- dCtBP mediates transcriptional repression by Knirps, Krüppel and Snail in the Drosophila embryoY Nibu
Department of Molecular and Cellular Biology, Division of Genetics, 401 Barker Hall, University of California, Berkeley, CA 94720, USA
EMBO J 17:7009-20. 1998..We propose that dCtBP represents a major form of transcriptional repression in development, and that the Groucho and dCtBP co-repressors mediate separate pathways of repression...
- Carbonyl reductase 1 is a predominant doxorubicin reductase in the human liverNina Kassner
Department of Pharmacology, Johannes Gutenberg University Mainz, Obere Zahlbacher Str 67, D 55101 Mainz, Germany
Drug Metab Dispos 36:2113-20. 2008..The variability in the CBR1 expression may affect outcomes of therapies with DOX, as well as with other CBR1 substrates...
- Improved synthesis of chiral alcohols with Escherichia coli cells co-expressing pyridine nucleotide transhydrogenase, NADP+-dependent alcohol dehydrogenase and NAD+-dependent formate dehydrogenaseAndrea Weckbecker
Institut für Molekulare Enzymtechnologie der Heinrich Heine Universität, Forschungszentrum Julich, Wilhelm Johnen Str, Julich, D 52426, Germany
Biotechnol Lett 26:1739-44. 2004..Cells that were permeabilized with toluene showed ketone reduction only if both cofactors were present...
- Pharmacological induction of leukotriene B4-12-hydroxydehydrogenase suppresses the oncogenic transformation of human hepatoma HepG2 cellsLai Wei
School of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 10 Sassoon Road, Pokfulam, Hong Kong, SAR, PR China
Int J Oncol 39:735-45. 2011..Importantly, pharmaco-logical induction of LTB4DH represents a potential alternative strategy for the therapy of hepatocellular carcinoma...
- Enhanced production of 2,3-butanediol by engineered Bacillus subtilisRanjita Biswas
Organization of Advance Science and Technology, Kobe University, Kobe 657 8501, Japan
Appl Microbiol Biotechnol 94:651-8. 2012..1 g/l and overall productivity increased by 6.7-fold to 0.4 g/l h in the engineered strain compared to that in the parental control...
- Limited roles of Rdh8, Rdh12, and Abca4 in all-trans-retinal clearance in mouse retinaAkiko Maeda
Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106 4965, USA
Invest Ophthalmol Vis Sci 50:5435-43. 2009..The protective roles were examined of the all-trans-RDHs, Rdh8 and Rdh12, and the ATP-binding cassette transporter Abca4, retinoid cycle enzymes involved in all-trans-retinal clearance...
- Secondary alcohol dehydrogenase catalyzes the reduction of exogenous acetone to 2-propanol in Trichomonas vaginalisRobert Sutak
Department of Parasitology, Faculty of Science, Charles University in Prague, Vinicna 7, Prague, Czech Republic
FEBS J 279:2768-80. 2012..vaginalis to modulate the metabolic fluxes indicate efficacious metabolic responsiveness that could be advantageous for rapid adaptation of the parasite to changes in the host environment...
- Reconstruction of human hepatocyte glyoxylate metabolic pathways in stably transformed Chinese-hamster ovary cellsJoseph T Behnam
Department of Biology, University College London, London WC1E 6BT, UK
Biochem J 394:409-16. 2006..These CHO cell transformants have a potential use as a cell-based bioassay for screening small molecules that stabilize AGT or GR/HPR and might have use in the treatment of PH1 or PH2...
- Genome sequence of thermotolerant Bacillus methanolicus: features and regulation related to methylotrophy and production of L-lysine and L-glutamate from methanolTonje M B Heggeset
SINTEF Materials and Chemistry, Department of Biotechnology, SINTEF, Trondheim, Norway
Appl Environ Microbiol 78:5170-81. 2012..methanolicus and open up possibilities for system-level metabolic engineering of this bacterium for the production of amino acids and other useful compounds from methanol...
- Structural and mechanistic similarities of 6-phosphogluconate and 3-hydroxyisobutyrate dehydrogenases reveal a new enzyme family, the 3-hydroxyacid dehydrogenasesJ W Hawes
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, 46202 5122, USA
FEBS Lett 389:263-7. 1996..The data suggest that 3-hydroxyisobutyrate and 6-phosphogluconate dehydrogenases may comprise, in part, a previously unrecognized family of 3-hydroxyacid dehydrogenases...
- Identification of a gatekeeper residue that prevents dehydrogenases from acting as oxidasesNicole G H Leferink
Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands
J Biol Chem 284:4392-7. 2009..The presence of such an oxygen access gate seems to be a key factor for the prevention of oxidase activity within the VAO family and is absent in members that act as oxidases...
- Signatures of cinnamyl alcohol dehydrogenase deficiency in poplar ligninsCatherine Lapierre
UMR 206 INRA INAPG Chimie Biologique, Institut National Agronomique, F 78850 Thiverval Grignon, France
Phytochemistry 65:313-21. 2004....
- Transcriptional control of the dihydroflavonol 4-reductase multigene family in Lotus japonicusKazuko Yoshida
Department of Biological Sciences, Ochanomizu University, 2 1 1 Otsuka, Bunkyo ku, Tokyo, 112 8610, Japan
J Plant Res 123:801-5. 2010..Ectopic expression of a combination of the transcription factors MYB, bHLH, and WDR showed that only the DFR2 promoter was activated, indicating that each member of the DFR gene family is regulated independently...
- Plasmid-dependent methylotrophy in thermotolerant Bacillus methanolicusTrygve Brautaset
Department of Biotechnology, Norwegian University of Science and Technology, N 7491 Trondheim SINTEF Applied Chemistry, SINTEF, N 7043 Trondheim, Norway
J Bacteriol 186:1229-38. 2004..Our findings should have an effect on future genetic manipulations of this organism, and they contribute to a new understanding of the biology of methylotrophs...
- X-ray structure of the quinoprotein ethanol dehydrogenase from Pseudomonas aeruginosa: basis of substrate specificityT Keitel
Universitätsklinikum Charité Institut für Biochemie, Humboldt Universitat zu Berlin, Monbijoustr 2, Berlin, D 10117, Germany
J Mol Biol 297:961-74. 2000..In addition to the Ca(2+) in the active-site cavity found also in methanol dehydrogenase, ethanol dehydrogenase contains a second Ca(2+)-binding site at the N terminus, which contributes to the stability of the native enzyme...
- ZEB represses transcription through interaction with the corepressor CtBPA A Postigo
Division of Molecular Oncology, Departments of Medicine and Cell Biology, Washington University School of Medicine, St Louis, MO 63110, USA
Proc Natl Acad Sci U S A 96:6683-8. 1999..Finally, we demonstrate that the interaction of CtBP with ZEB at the promoter is necessary for repressor activity...
- Functional analysis of a cinnamyl alcohol dehydrogenase involved in lignin biosynthesis in wheatQing Hu Ma
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxin Cun, Xiangshan, Beijing, China
J Exp Bot 61:2735-44. 2010..These properties were correlated to the lignin contents and lodging indices of the two cultivars. These data suggest that TaCAD1 is the predominant CAD in wheat stem for lignin biosynthesis and is critical for lodging resistance...
- RIBEYE, a component of synaptic ribbons: a protein's journey through evolution provides insight into synaptic ribbon functionF Schmitz
Howard Hughes Medical Institute, Center for Basic Neuroscience, Department of Molecular Genetics, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Neuron 28:857-72. 2000..Our results define a unique component of synaptic ribbons and suggest that RIBEYE evolved in vertebrates under utilization of a preexisting protein to build a unique scaffold for a specialized synapse...
- An alcohol oxidase biosensor using PNR redox mediator at carbon film electrodesMadalina M Barsan
Departamento de Quimica, Universidade de Coimbra, 3004 535 Coimbra, Portugal
Talanta 74:1505-10. 2008..No significant interferences were found from compounds usually present in wine. The biosensor was used for the determination of ethanol in Portuguese red and white wines...
- Cone and rod dysfunction in fundus albipunctatus with RDH5 mutation: an electrophysiological studyYasuhiro Niwa
Department of Ophthalmology, Nagoya University School of Medicine, Nagoya, Japan
Invest Ophthalmol Vis Sci 46:1480-5. 2005..The purpose of this study was to investigate the frequency of cone dysfunction and to determine the cause of the reduced full-field cone ERGs in patients with FA and whether the rod system is affected in patients with FA...
- Ancestral gene fusion in cellobiose dehydrogenases reflects a specific evolution of GMC oxidoreductases in fungiMarcel Zamocky
Division of Food Biotechnology, Department of Food Science and Technology, BOKU University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, A 1190 Wien, Austria
Gene 338:1-14. 2004..This is the first attempt towards a comprehensive phylogenetic analysis of cellobiose dehydrogenases...
- A novel RDH5 gene mutation in a patient with fundus albipunctatus presenting with macular atrophy and fading white dotsHiroyuki Yamamoto
Departments of Organ Therapeutics, Division of Ophthalmology, Kobe University Graduate School of Medicine, Japan
Am J Ophthalmol 136:572-4. 2003..To report a novel homozygous RDH5 gene mutation in a 76-year-old fundus albipunctatus who developed macular atrophy with the disappearance of white dots...
- Elements in the N-terminal signaling sequence that determine cytosolic topology of short-chain dehydrogenases/reductases. Studies with retinol dehydrogenase type 1 and cis-retinol/androgen dehydrogenase type 1Min Zhang
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720 3104, USA
J Biol Chem 279:51482-9. 2004....
- Antagonistic interaction between abscisic acid and jasmonate-ethylene signaling pathways modulates defense gene expression and disease resistance in ArabidopsisJonathan P Anderson
Cooperative Research Centre for Tropical Plant Protection, University of Queensland, St Lucia, Queensland, 4072, Australia
Plant Cell 16:3460-79. 2004....
- Identification of RDH10, an All-trans Retinol Dehydrogenase, in Retinal Muller CellsBill X Wu
Department of Ophthalmology, Medical University of South Carolina, USA
Invest Ophthalmol Vis Sci 45:3857-62. 2004..To investigate the expression of RDH10, an all-trans retinol dehydrogenase identified in the retinal pigment epithelium (RPE), in retinal Muller cells...
- Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein (ACP) reductase: kinetic and chemical mechanismsRafael G Silva
Centro de Pesquisas em Biologia Molecular e Funcional, Faculdade de Biociências e Faculdade de Farmácia, Instituto de Pesquisas Biomédicas, PUCRS, 6681 92 A Avenida Ipiranga, 90619 900, Porto Alegre, RS, Brazil
Biochemistry 45:13064-73. 2006..Kinetic and chemical mechanisms for the MabA-catalyzed reaction are proposed on the basis of the experimental data...
- Partial biopterin deficiency disturbs postnatal development of the dopaminergic system in the brainDaigo Homma
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226 8501, Japan
J Biol Chem 286:1445-52. 2011..The developmental manifestation of psychomotor symptoms in BH4 deficiency might be attributable at least partially to high dependence of dopaminergic development on BH4 availability...
- Identification and characterization of a stereospecific human enzyme that catalyzes 9-cis-retinol oxidation. A possible role in 9-cis-retinoic acid formationJ R Mertz
Department of Cell Biology and Anatomical Sciences, City University of New York Medical School, New York, New York 10031, USA
J Biol Chem 272:11744-9. 1997..We propose that this cDNA encodes a previously unknown stereospecific enzyme, 9-cis-retinol dehydrogenase, which probably plays a role in 9-cis-retinoic acid formation...
- Phenotypic variability in RDH5 retinopathy (Fundus Albipunctatus)Panagiotis I Sergouniotis
Moorfields Eye Hospital, London, United Kingdom Institute of Ophthalmology, University College London, London, UK
Ophthalmology 118:1661-70. 2011..To describe phenotypic variability and report novel mutational data in patients with mutation in RDH5 (fundus albipunctatus)...
- Lack of autofluorescence in fundus albipunctatus associated with mutations in RDH5Patrik Schatz
Department of Ophthalmology, Glostrup Hospital, University of Copenhagen, Copenhagen, Denmark
Retina 30:1704-13. 2010..The purpose of this study was to characterize the phenotype of fundus albipunctatus associated with RDH5 mutations...
- XoxF is required for expression of methanol dehydrogenase in Methylobacterium extorquens AM1Elizabeth Skovran
Department of Chemical Engineering, University of Washington, Seattle, Washington 98195 2180, USA
J Bacteriol 193:6032-8. 2011....
- RDH10 is the primary enzyme responsible for the first step of embryonic Vitamin A metabolism and retinoic acid synthesisKrysten M Farjo
Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
Dev Biol 357:347-55. 2011..This provides a breakthrough in understanding the precise cellular mechanisms that regulate Vitamin A metabolism and the synthesis of the essential embryonic regulatory molecule atRA...
- Complete kinetic mechanism of homoisocitrate dehydrogenase from Saccharomyces cerevisiaeYing Lin
Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, Oklahoma 73019, USA
Biochemistry 46:890-8. 2007..The Keq for the overall reaction measured directly using the change in NADH as a probe is 0.45 M...
- Null mutation in the human 11-cis retinol dehydrogenase gene associated with fundus albipunctatusC A Driessen
Department of Ophthalmology, University of Nijmegen, Nijmegen, The Netherlands
Ophthalmology 108:1479-84. 2001..The authors wanted to investigate whether additional, more severe, mutations in the 11-cis retinol dehydrogenase gene might be responsible for more severe forms of hereditary retinal diseases...
- Multiple pathways guide oxygen diffusion into flavoenzyme active sitesRiccardo Baron
Department of Chemistry and Biochemistry and Pharmacology, Center for Theoretical Biological Physics, and Howard Hughes Medical Institute, University of California at San Diego, La Jolla, CA 92093 0365, USA
Proc Natl Acad Sci U S A 106:10603-8. 2009..The difference in O(2) reactivity among dehydrogenases, monooxygenases, and oxidases ultimately resides in the fine modulation of the local environment embedding the reactive locus of the flavin...
- Phylogeny and expression profiling of CAD and CAD-like genes in hybrid Populus (P. deltoides x P. nigra): evidence from herbivore damage for subfunctionalization and functional divergenceAbdelali Barakat
The School of Forest Resources, and The Huck Institutes of the Life Sciences, Pennsylvania State University, 324 Forest Resources Building, University Park, PA 16802, USA
BMC Plant Biol 10:100. 2010..Expression of CAD/CAD-like genes in Populus tissues (xylem, leaves, and barks) was analyzed in herbivore-treated and non-treated plants by real time quantitative RT-PCR...
- The primary structure of spinach glycolate oxidase deduced from the DNA sequence of a cDNA cloneM Volokita
MSU DOE Plant Research Laboratory, East Lansing 48824
J Biol Chem 262:15825-8. 1987..Comparison of the deduced amino acid sequence with those of two other plant peroxisomal proteins revealed a region of homology which may be involved in directing proteins to the peroxisome...
- Purification and characterization of vanillyl-alcohol oxidase from Penicillium simplicissimum. A novel aromatic alcohol oxidase containing covalently bound FADE de Jong
Department of Food Science, Agriculture University, Wageningen, The Netherlands
Eur J Biochem 208:651-7. 1992..Photoreduction of the enzyme in the presence of the competitive inhibitor cinnamyl alcohol gives rise to a complete, irreversible bleaching of the flavin spectrum...
- A novel compound heterozygous mutation in the RDH5 gene in a patient with fundus albipunctatusS Kuroiwa
Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan
Am J Ophthalmol 130:672-5. 2000..To report a novel compound heterozygous mutation in the 11-cis retinol dehydrogenase (RDH5) gene in a patient with fundus albipunctatus...
- Young monozygotic twin sisters with fundus albipunctatus and cone dystrophyMakoto Nakamura
Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
Arch Ophthalmol 122:1203-7. 2004....
- The 11-cis-retinol dehydrogenase activity of RDH10 and its interaction with visual cycle proteinsKrysten M Farjo
Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA
Invest Ophthalmol Vis Sci 50:5089-97. 2009..This study was conducted to characterize the 11-cis-RDH activity of RDH10 in vitro and specifically to determine whether RDH10 can functionally and physically interact with visual cycle proteins...
- Macular dystrophy in a Japanese family with fundus albipunctatusKazuki Hotta
Department of Ophthalmology, Kameda Medical Center, Kamogowa, Japan
Am J Ophthalmol 135:917-9. 2003..To report a Japanese family with fundus albipunctatus and macular dystrophy associated with a mutation in the 11-cis retinol dehydrogenase (RDH5) gene...
- Functional investigation of methanol dehydrogenase-like protein XoxF in Methylobacterium extorquens AM1Sabrina Schmidt
Institute of Microbiology, ETH Zurich, Wolfgang Pauli Strasse 10, 8093 Zurich, Switzerland
Microbiology 156:2575-86. 2010..Determination of the kinetic properties of the purified enzyme showed a conversion capacity for both formaldehyde and methanol. The results suggest that XoxF is involved in one-carbon metabolism in M. extorquens AM1...
- [Clinical and genetic findings in a patient with fundus albipunctatus]K Rüther
Charite, Universitatsmedizin Berlin, Augenklinik Campus Virchow Klinikum
Ophthalmologe 101:177-85. 2004..She was screened for mutations in exons 2-5 and exon/intron boundaries of the 11- cis retinol dehydrogenase gene by direct sequencing...
- L: -2-Hydroxyglutaric aciduria, a disorder of metabolite repairE Van Schaftingen
de Duve Institute, Universite Catholique de Louvain, Brussels, Belgium
J Inherit Metab Dis 32:135-42. 2009..Its accumulation is toxic to the mammalian brain, causing a leukoencephalopathy and increasing the susceptibility to develop tumours. L: -2-Hydroxyglutaric aciduria appears to be the first disease of 'metabolite repair'...
- Smad6 recruits transcription corepressor CtBP to repress bone morphogenetic protein-induced transcriptionXia Lin
Michael E DeBakey Department of Surgery, Baylor College of Medicine, One Baylor Plaza, Room 131D, Houston, TX 77030, USA
Mol Cell Biol 23:9081-93. 2003..We conclude that the nuclear functions and physical interaction of Smad6 and CtBP provide a novel mechanism for the transcriptional regulation by inhibitory Smads...
- Diagnosis in a patient with fundus albipunctatus and atypical fundus changesManal Hajali
Department of Ophthalmology and Visual Sciences MC 648, Eye and Ear Infirmary, University of Illinois at Chicago, 1855 W Taylor Street, Chicago, IL 60612 7234, USA
Doc Ophthalmol 118:233-8. 2009..Genetic testing and extended ERG and psychophysical testing may be necessary to diagnose FA from early stages of progressive RPA...
- Fundus albipunctatus in a 6-year old girl due to compound heterozygous mutations in the RDH5 geneAlessandro Iannaccone
Retinal Degeneration and Ophthalmic Genetics Service, Hamilton Eye Institute, Department of Ophthalmology, University of Tennessee Health Science Center, 930 Madison Avenue, Suite 731, Memphis, TN 38163, USA
Doc Ophthalmol 115:111-6. 2007..The behavior of dark-adapted ERG responses in FA displays characteristics that differ from those of RPA patients, which may be useful to differentiate functionally these two conditions at their common albipunctate stages...
- Structural comparison of the Pichia pastoris alcohol oxidase genesP Koutz
Salk Institute Biotechnology Industrial Associates, Inc, La Jolla, CA 92037
Yeast 5:167-77. 1989..pastoris, the AOX amino acid sequences did not contain a peptide sequence similar to the peroxisomal transport sequence found at the C-terminus of some peroxisomally located proteins in higher eukaryotes...
- PHARMACOGENETICS OF HUMAN CARBONYL REDUCTASESJAVIER GUILLERMO BLANCO; Fiscal Year: 2013..The body of knowledge gathered from the proposed research will contribute to the development of anticancer therapy that can be individualized by identifying the genetic determinants of variable CBR activity. ..
- Bupropion for smoking cessation during pregnancyTatiana Nanovskaya; Fiscal Year: 2013..In summary, The information obtained is necessary for evaluating bupropion as a potential medication for aiding in smoking cessation during pregnancy. ..
- Anthracycline Specificities of Carbonyl ReductasesHENRY CHARLIER; Fiscal Year: 2003..The information from this proposal may be used to improve the use of anthracyclines in the treatment of cancer by leading to treatments that greatly reduce the threat of heart damage. ..
- Optimizing Escherichia Coli for Carbonyl reductionJAMES ROZZELL; Fiscal Year: 2004..coli host will be used as whole cell catalyst to demonstrate the commercial potential of this strain by producing a 100 g of (S)-ethyl 4-chloro-3-hydroxybutyrate, a key intermediate for anti-cholesterol drug Lipitor. ..
- Apoptosis Signaling: BH3 to BH123 ProteinsGOVINDASWAMY CHINNADURAI; Fiscal Year: 2008..The knowledge gained would be valuable in modulating the activity of BAX in cancer and in degenerative diseases. ..
- Molecular Mechanisms of Multistage CarcinogenesisTREVOR PENNING; Fiscal Year: 2006..By studying the discrete molecular events responsible for the causation of cancer this Program may lead to the early prevention and intervention of this disease. ..
- BH3-only protein BNIP3 in tumor progressionGOVINDASWAMY CHINNADURAI; Fiscal Year: 2006..Our results may also provide the experimental support to suggest that BNIP3 may be a new prognostic marker for human cancer. [unreadable] [unreadable]..
- Symposium on Aldo-Keto Reductases & Toxicant MetabolismTREVOR PENNING; Fiscal Year: 2002..abstract_text> ..
- Regulation of Evi-1 function via the corepressor CtBPQinghong Zhang; Fiscal Year: 2006..The mentor's laboratory and institution are rich in intellectual and physical resources that provide an excellent environment for facilitating the principal investigator's transition to independent scientist. ..
- Liver Fibrosis, Inflammation & Oxidative Stress MarkersCharles Lieber; Fiscal Year: 2006..abstract_text> ..
- Antiviral/Antifibrotic Liver Therapy of Hepatitis C positive Alcohol DrinkersCharles Lieber; Fiscal Year: 2007..Accordingly, support is requested for the completion of this novel approach for the treatment of HCV+ patients who become abstinent or who continue to consume alcohol moderately. [unreadable] [unreadable]..
- Synergistic Nutraceutical Effects of DLPC and SAMeCharles Lieber; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- MECHANISM AND ROLE OF MICROSOMAL ETHANOL OXIDATIONCharles Lieber; Fiscal Year: 2001..Such information is aimed at elucidating key aspects of alcohol mediated pathology in a way which may eventually affect alcohol related medical complications, their prevention and treatment. ..
- GENETIC DISSECTION OF ALCOHOL/RETINOL METABOLIC PATHWAYSGregg Duester; Fiscal Year: 2002..3) Prepare and ALDH-I knockout mouse plus mice mutated for one or more ADHs and ALDH-I, then analyze their phenotype as above. ..
- Retinoid Dehydrogenases Involved in Eye DevelopmentGREGG L DUESTER; Fiscal Year: 2010....
- DLPC Treatment of Alcoholic and Non-Alcoholic FibrosisCharles Lieber; Fiscal Year: 2006..abstract_text> ..
- ANTIVIRAL & ANTIFIBROTIC LIVER THERAPY OF HCV+ DRINKERSCharles Lieber; Fiscal Year: 2005..Funds are requested for special laboratory tests, study nurses, travel to meetings, patient monitoring expenses and a core office. ..
- Xenogen Rodent Imaging SystemJOSEPH NAPOLI; Fiscal Year: 2008..Availability of this instrument would undoubtedly stimulate its use by others on campus and in the area. [unreadable] [unreadable] [unreadable]..
- Iron Homeostasis and its Involvement in C1 MetabolismElizabeth Skovran; Fiscal Year: 2007..extorquens AM1. [unreadable] [unreadable] [unreadable]..
- Influence of genotype on urinary oxalate excretionCARLA MONICO; Fiscal Year: 2007..The applicant's long-term objective is to establish an investigative state-of the- art laboratory devoted to the study of the molecular genetics of hyperoxaluria. ..
- Development of selenomethionine resistant yeastTERRI KINZY; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Alopecia and Ulcerative Dermatitis in B6 MiceJohn Sundberg; Fiscal Year: 2007..Future work will apply findings to human patients to either modify lifestyle or more appropriately treat CCCA to reduce suffering and disfiguration. [unreadable] [unreadable] [unreadable]..
- Biosynthesis of 9-cis-retinoic acid/retinol homeostasisJOSEPH NAPOLI; Fiscal Year: 2007..This work will provide new insight into retinoid metabolism/ function. ..
- RETINOID METABOLISMJOSEPH NAPOLI; Fiscal Year: 2007..This work will contribute to revealing pathology caused or exacerbated by impaired atRA biosynthesis during carcinogenesis, skin disease, birth defects, or diseases of intermediary metabolism. ..
- Vitamin D and Soy Isoflavone Inhibition of ProstateScott Cramer; Fiscal Year: 2008..Our long-term goals are to develop a mechanistic-based chemoprevention strategy that utilizes dietary supplementation with vitamin D and soy isoflavanoid. [unreadable] [unreadable] [unreadable]..
- PATHOGENESIS OF RETINAL DEGENERATIONSSamuel Jacobson; Fiscal Year: 2001..The results of this research will lead to the formulation of hypotheses about the underlying molecular events in these diseases and may evolve into recommendations for treatment paradigms in these blinding retinal degenerations. ..
- PcG Function of YY1 in Transcription and DevelopmentMichael L Atchison; Fiscal Year: 2010..PcG proteins are also implicated in muscle differentiation. Therefore, we will test the roles of YY1, CtBP, SUMO-1, and PCNA in differentation of myblasts into myotubes. ..
- MECHANISMS AND CONSEQUENCES OF DELETERIOUS EVOLUTION IN BACTERIAJennifer J Wernegreen; Fiscal Year: 2010..By deciphering fundamental mechanisms that influence bacterial genome evolution, this study will contribute to a predictive framework for understanding how microbes evolve. ..
- Epithelial Cell Structure in Vertebrate DevelopmentJeffrey D Hildebrand; Fiscal Year: 2010..We will continue to use in vivo studies in mice and in vitro studies in cell culture to understand the molecular mechanisms underlying human birth defects. ..
- Halogenated Alkenes and Microsomal GSH-transferasesMichael J Kelner; Fiscal Year: 2010..4] To determine the relative contribution of MGST1 and MGST2 to cellular antioxidant capacity through studies utilizing human MGST1 and MGST2 null cells. ..
- AUTOMATED DNA ANALYSIS SYSTEMTERRI KINZY; Fiscal Year: 2001..Trained personnel of the core facility will run and maintain all equipment to ensure optimal quality and speed. These personnel will also provide ongoing technical support to all users of this equipment. ..
- ROLE OF THE BCL 6 PROTO ONCOGENE IN B CELL LYMPHOMASBIHUI HILDA YE; Fiscal Year: 2010..abstract_text> ..
- Expression of Human Therapeutic Proteins in Transgenic Tobacco ChloroplastsHenry Daniell; Fiscal Year: 2010..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..
- RETINAL DEHYDROGENASESJOSEPH NAPOLI; Fiscal Year: 2009..The fundamental insight and reagents generated will be useful to identify diseases and age-related degeneration that may be caused or exacerbated by impaired RA biosynthesis. ..
- RESEARCH TRAINING FOR VETERINARIANS: ANIMAL MODELSJohn Sundberg; Fiscal Year: 2008..The investigators ask to maintain the three slots currently funded. This program is designed to meet the increasing demand for scientists in functional genomics research. ..
- Regulation of SMAD2 Signaling by SMP1 PhosphataseXia Lin; Fiscal Year: 2008..The results will be pertinent towards development for a foundation for the rational design of novel therapeutic approaches for prevention and treatment of human cancers and other diseases. ..
- Drosophila energetics and mito-nuclear functionKristi Montooth; Fiscal Year: 2007..The results will identify candidate enzyme-encoding and regulatory genes that can be further investigated for functional roles in maintaining metabolic health. [unreadable] [unreadable]..
- GPX1 ENZYME REGULATION BY OXIDATIVE XENOBIOTICSMichael Kelner; Fiscal Year: 2002..These studies will provide critical information regarding cellular response to oxidative stress and aid in determining if a common regulatory mechanism exists for GSH-dependent enzymes. ..
- COORDINATED REGULATION OF ANTIOXIDANT ENZYMESMichael Kelner; Fiscal Year: 2005..abstract_text> ..
- Clinical Phenotype of Imprinted Genes of Chromosome 14Vernon Sutton; Fiscal Year: 2005..This phenotype delineation will lay the foundation for understanding the effects and pathogenesis of imprinted genes on chromosome 14. ..
- A STANDARD STRUCTURE AND FUNCTION FOR WD REPEAT PROTEINSDavid Wilson; Fiscal Year: 2005..abstract_text> ..
- Development of DHPS as a Bioterrorism Therapeutic TargetStephen White; Fiscal Year: 2005..The institutions have complementary programs in infectious diseases with a strong emphasis on studying bioterrorism. ..
- Novel peroxisomal genes in the yeast Pichia pastorisJames Cregg; Fiscal Year: 2005..P. pastoris strains deleted for each strain will be constructed and examined biochemically and morphologically for peroxisome function to identify new PEX genes and to define their role in peroxisome biogenesis. ..
- Cell Biology of Human Thiamine TransportersVEEDAMALI SUBRAMANIAN; Fiscal Year: 2005....
- DEVELOPMENTAL CONTROL OF IMMUNOGLOBULIN TRANSCRIPTIONMichael Atchison; Fiscal Year: 2005..These studies represent parallel in vitro and in vivo approaches to study gene regulation and cellular differentiation. ..
- Structure of Eukaryotic Translation Elongation Factor 1TERRI KINZY; Fiscal Year: 2004..With S. cerevisiae, the structural information obtained on eEF1A can be utilized for molecular genetic analysis of the critical residues for function and regulation. ..
- TRAINING IN SEQUENCE ANALYSIS USING SUPERCOMPUTERSHUGH NICHOLAS; Fiscal Year: 2003..Future editions of the course will also include information on the computational complexities of analyzing complete genomes and microarray data. ..
- Effect of hyperoxaluria on gene expression in the kidneyGill Rumsby; Fiscal Year: 2005..By understanding why the disease progresses at different rates in patients with the same genetic abnormality, it may then be possible to devise strategies to slow down or prevent the clinical consequences of the disease. ..
- Prostate Specific Antigen and Prostate Ca. ProgressionScott Cramer; Fiscal Year: 2005..In addition, the identification of PSA as a modulator of prostate cancer aggressiveness may change the way that the PSA test is used for prostate cancer screening. ..
- GPHRP KNOCKOUT MOUSE AS A MODEL OF HYPEROXALURIAScott Cramer; Fiscal Year: 2003..A GRHPR knockout mouse that exhibits hyperoxaluria will be useful for the future development of novel therapeutic strategies for PH2 and for elucidating the tissue source(s) of oxalate in PH2 patients. ..
- RPE65 IN RETINAL METABOLISM AND DEGENERATIONDebra Thompson; Fiscal Year: 2005..The long-term goals of this project are to elucidate the mechanisms by which RPE65 defects contribute to retinal degeneration, and to lay the groundwork for the development of therapeutic approaches to the disease. ..
- STRUCTURAL STUDIES ON RIBOSOMAL COMPONENTSStephen White; Fiscal Year: 2002..Future work will improve this image to accurately orient the IF3 molecule, and additional factors will be added to build up the complete initiation complex. ..
- STRUCTURE AND FUNCTION OF MITOTIC CHECKPOINT PROTEINSDavid Wilson; Fiscal Year: 2002..This structure will provide an understanding of the effects of any cancer-causing mutations. It will later be used to analyze interactions with other human mitotic checkpoint control proteins. ..
- MOLECULAR MECHANISMS OF TRANSMITTER RELEASE MODULATIONKim Mitchell; Fiscal Year: 2002..Proposed in vivo measurements of NO diffusion should yield novel information about the magnitude of physiological changes in NO concentration and the spatial and temporal sphere of NO influence. ..
- PATHOLOGY OF THE LABORATORY MOUSEJohn Sundberg; Fiscal Year: 2006..The Workshop will accommodate 16 participants who will have an opportunity to interact with a group of prominent mouse pathologists and geneticists from both TJL, and other leading institutions. ..
- Mitochondrial GRHPR and HyperoxaluriaScott Cramer; Fiscal Year: 2006..The studies in this proposal could uncover the molecular basis of UncPH, develop new screening tools for PH, and elucidate basic mechanisms that dictate the intracellular targeting of GRHPR. [unreadable] [unreadable] [unreadable]..
- Roles of Oct4 dimers during mouse germline developmentMichael Atchison; Fiscal Year: 2006..The anticipated results will have relevant applicability to stem cell and germline biology and lead to a better understanding of human reproduction. ..
- Control of SnoN Activity by SUMO modificationXia Lin; Fiscal Year: 2006..Finally, the results may also provide a foundation for the rational design of novel therapeutic approaches for prevention and treatment of cancer and musculoskeletal diseases. [unreadable] [unreadable]..
- VPR-INDUCED ENDOTHELIAL CYTOSKELETAL/FUNCTIONAL CHANGESLing Jun Zhao; Fiscal Year: 2002..We anticipate that this project will generate novel knowledge about the mechanism of Vpr induced cytoskeletal/functional changes in endothelial cells that can benefit the designing of therapeutic strategies. ..
- MOLECULAR STRUCTURE OF STEROIDSWilliam Duax; Fiscal Year: 2006..Proof of the successful prediction of the structures of 20-30 hypothetical members of the SCOR proteome could provide a model for the prediction of protein folding on the basis of as little as 20% sequence homology. ..
- THE CARNITINE TRANSPORTER IN HUMAN DISEASENicola Longo; Fiscal Year: 2010....
- Anaplerotic therapy in Propionic AcidemiaNicola Longo; Fiscal Year: 2008..This approach, if effective, could be extended to a number of other diseases, including other organic acidemias and mitochondrial disorders. [unreadable] [unreadable] [unreadable]..