adenosylmethionine decarboxylase

Summary

Summary: An enzyme that catalyzes the decarboxylation of S-adenosyl-L-methionine to yield 5'-deoxy-(5'-),3-aminopropyl-(1), methylsulfonium salt. It is one of the enzymes responsible for the synthesis of spermidine from putrescine. EC 4.1.1.50.

Top Publications

  1. ncbi Targeting the polyamine biosynthetic enzymes: a promising approach to therapy of African sleeping sickness, Chagas' disease, and leishmaniasis
    O Heby
    Department of Molecular Biology, Umea University, Umea, Sweden
    Amino Acids 33:359-66. 2007
  2. pmc Inhibition of S-adenosylmethionine decarboxylase by inhibitor SAM486A connects polyamine metabolism with p53-Mdm2-Akt/protein kinase B regulation and apoptosis in neuroblastoma
    Dana Lynn T Koomoa
    Cancer Research Center of Hawaii, University of Hawaii at Manoa, Honolulu, HI 96813, USA
    Mol Cancer Ther 8:2067-75. 2009
  3. pmc Structural basis for putrescine activation of human S-adenosylmethionine decarboxylase
    Shridhar Bale
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA
    Biochemistry 47:13404-17. 2008
  4. pmc RNA interference-mediated silencing of ornithine decarboxylase and spermidine synthase genes in Trypanosoma brucei provides insight into regulation of polyamine biosynthesis
    Yanjing Xiao
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9041, USA
    Eukaryot Cell 8:747-55. 2009
  5. ncbi Cloning and sequencing of an intronless mouse S-adenosylmethionine decarboxylase gene coding for a functional enzyme strongly expressed in the liver
    K Persson
    Department of Cellular and Developmental Biology, University of Umea, Sweden
    J Biol Chem 270:5642-8. 1995
  6. doi Spermine facilitates recovery from drought but does not confer drought tolerance in transgenic rice plants expressing Datura stramonium S-adenosylmethionine decarboxylase
    Ariadna Peremarti
    Departament de Produccio Vegetal i Ciencia Forestal, ETSEA, Universitat de Lleida, Lleida, Spain
    Plant Mol Biol 70:253-64. 2009
  7. ncbi Enhanced susceptibility of photosynthesis to low-temperature photoinhibition due to interruption of chill-induced increase of S-adenosylmethionine decarboxylase activity in leaves of spinach (Spinacia oleracea L.)
    Lixiong He
    Department of Life Science, Faculty of Bioresources, Mie University, Tsu, 514 8507 Japan
    Plant Cell Physiol 43:196-206. 2002
  8. ncbi The functional intronless S-adenosylmethionine decarboxylase gene of the mouse (Amd-2) is linked to the ornithine decarboxylase gene (Odc) on chromosome 12 and is present in distantly related species of the genus Mus
    K Persson
    Department of Cellular and Developmental Biology, Umea University, S 901 87 Umea, Sweden
    Mamm Genome 10:784-8. 1999
  9. pmc Role of the sulfonium center in determining the ligand specificity of human s-adenosylmethionine decarboxylase
    Shridhar Bale
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA
    Biochemistry 48:6423-30. 2009
  10. ncbi Novel properties of malarial S-adenosylmethionine decarboxylase as revealed by structural modelling
    Gordon A Wells
    Department of Biochemistry, School of Biological Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South Africa
    J Mol Graph Model 24:307-18. 2006

Research Grants

  1. Polyamine Metabolism in Leishmania
    Buddy Ullman; Fiscal Year: 2006
  2. TRYPANOSMIASIS--MOLECULAR APPROACHES TO CHEMOTHERAPY
    Margaret Phillips; Fiscal Year: 2003
  3. Role of Polyamines in MYCN-Amplified Neuroblastoma
    ANDRE BACHMANN; Fiscal Year: 2009
  4. Role of Polyamines in MYCN-Amplified Neuroblastoma
    ANDRE BACHMANN; Fiscal Year: 2009
  5. Role of Polyamines in MYCN-Amplified Neuroblastoma
    ANDRE BACHMANN; Fiscal Year: 2007
  6. Targeting S-adenosylmethionine decarboxylase for HAT drug discovery
    Margaret A Phillips; Fiscal Year: 2010
  7. Polyamine and glutathione metabolism in trypanosomes.
    Margaret Phillips; Fiscal Year: 2007
  8. Rational Design of Inhibitors of Polyamine Synthesis
    Steven Ealick; Fiscal Year: 2007
  9. POLYAMINE METABOLISM IN TRYPANOSOMATIDS
    Buddy Ullman; Fiscal Year: 2001
  10. GROWTH REGULATION OF POLYAMINE SYNTHESIS
    David Morris; Fiscal Year: 2001

Detail Information

Publications214 found, 100 shown here

  1. ncbi Targeting the polyamine biosynthetic enzymes: a promising approach to therapy of African sleeping sickness, Chagas' disease, and leishmaniasis
    O Heby
    Department of Molecular Biology, Umea University, Umea, Sweden
    Amino Acids 33:359-66. 2007
    ..e. arginase, ornithine decarboxylase (ODC), S-adenosylmethionine decarboxylase (AdoMetDC), spermidine synthase, trypanothione synthetase (TryS), and trypanothione reductase (TryR), ..
  2. pmc Inhibition of S-adenosylmethionine decarboxylase by inhibitor SAM486A connects polyamine metabolism with p53-Mdm2-Akt/protein kinase B regulation and apoptosis in neuroblastoma
    Dana Lynn T Koomoa
    Cancer Research Center of Hawaii, University of Hawaii at Manoa, Honolulu, HI 96813, USA
    Mol Cancer Ther 8:2067-75. 2009
    S-adenosylmethionine decarboxylase (AdoMetDC) is an essential enzyme of polyamine (PA) biosynthesis, and both AdoMetDC and PA levels are often up-regulated in cancer cells...
  3. pmc Structural basis for putrescine activation of human S-adenosylmethionine decarboxylase
    Shridhar Bale
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA
    Biochemistry 47:13404-17. 2008
    Putrescine (1,4-diaminobutane) activates the autoprocessing and decarboxylation reactions of human S-adenosylmethionine decarboxylase (AdoMetDC), a critical enzyme in the polyamine biosynthetic pathway...
  4. pmc RNA interference-mediated silencing of ornithine decarboxylase and spermidine synthase genes in Trypanosoma brucei provides insight into regulation of polyamine biosynthesis
    Yanjing Xiao
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9041, USA
    Eukaryot Cell 8:747-55. 2009
    ..Recently, we showed that RNA interference (RNAi)-mediated gene silencing or the inhibition of S-adenosylmethionine decarboxylase (AdoMetDC) led to the upregulation of the AdoMetDC activator, prozyme, and ornithine decarboxylase (..
  5. ncbi Cloning and sequencing of an intronless mouse S-adenosylmethionine decarboxylase gene coding for a functional enzyme strongly expressed in the liver
    K Persson
    Department of Cellular and Developmental Biology, University of Umea, Sweden
    J Biol Chem 270:5642-8. 1995
    A genomic clone for a mouse S-adenosylmethionine decarboxylase (AdoMetDC) gene was isolated from a cosmid library. Surprisingly, the gene proved to be intronless...
  6. doi Spermine facilitates recovery from drought but does not confer drought tolerance in transgenic rice plants expressing Datura stramonium S-adenosylmethionine decarboxylase
    Ariadna Peremarti
    Departament de Produccio Vegetal i Ciencia Forestal, ETSEA, Universitat de Lleida, Lleida, Spain
    Plant Mol Biol 70:253-64. 2009
    ..and spermine, we generated transgenic rice plants constitutively expressing a heterologous S-adenosylmethionine decarboxylase (SAMDC) gene from Datura stramonium so that spermidine and spermine levels could be investigated ..
  7. ncbi Enhanced susceptibility of photosynthesis to low-temperature photoinhibition due to interruption of chill-induced increase of S-adenosylmethionine decarboxylase activity in leaves of spinach (Spinacia oleracea L.)
    Lixiong He
    Department of Life Science, Faculty of Bioresources, Mie University, Tsu, 514 8507 Japan
    Plant Cell Physiol 43:196-206. 2002
    ..was investigated focusing on photosynthesis. During chilling at 8/5C (day/night) for 6 d, S-adenosylmethionine decarboxylase (SAMDC) activity increased significantly in leaves in parallel with the increase in putrescine and ..
  8. ncbi The functional intronless S-adenosylmethionine decarboxylase gene of the mouse (Amd-2) is linked to the ornithine decarboxylase gene (Odc) on chromosome 12 and is present in distantly related species of the genus Mus
    K Persson
    Department of Cellular and Developmental Biology, Umea University, S 901 87 Umea, Sweden
    Mamm Genome 10:784-8. 1999
    S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the biosynthesis of polyamines...
  9. pmc Role of the sulfonium center in determining the ligand specificity of human s-adenosylmethionine decarboxylase
    Shridhar Bale
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA
    Biochemistry 48:6423-30. 2009
    S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the polyamine biosynthetic pathway...
  10. ncbi Novel properties of malarial S-adenosylmethionine decarboxylase as revealed by structural modelling
    Gordon A Wells
    Department of Biochemistry, School of Biological Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South Africa
    J Mol Graph Model 24:307-18. 2006
    ..the two main regulatory activities of polyamine biosynthesis, ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) occur in a single bifunctional protein...
  11. ncbi Thymosin beta4 is a determinant of the transformed phenotype and invasiveness of S-adenosylmethionine decarboxylase-transfected fibroblasts
    Pirjo Nummela
    Haartman Institute and Helsinki University Central Hospital, Department of Pathology, University of Helsinki, FIN 00014 Helsinki, Finland
    Cancer Res 66:701-12. 2006
    S-adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the synthesis of polyamines essential for cell growth and proliferation...
  12. ncbi S-adenosyl methionine decarboxylase activity is required for the outcome of herpes simplex virus type 1 infection and represents a new potential therapeutic target
    Anna Greco
    INSERM U369, Faculté de Médecine Lyon R T H Laennec, Lyon, France
    FASEB J 19:1128-30. 2005
    ..Our data provide the opportunity to develop molecules with a novel mechanism of action for the treatment of herpes infection...
  13. pmc Parasite-specific inserts in the bifunctional S-adenosylmethionine decarboxylase/ornithine decarboxylase of Plasmodium falciparum modulate catalytic activities and domain interactions
    Lyn Marie Birkholtz
    Department of Biochemistry, School of Biological Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, 0002, South Africa
    Biochem J 377:439-48. 2004
    ..falciparum AdoMetDC (S-adenosylmethionine decarboxylase)/ODC (ornithine decarboxylase)] with a molecular mass of 330 kDa...
  14. ncbi Antileishmanial effect of a potent S-adenosylmethionine decarboxylase inhibitor: CGP 40215A
    R Mukhopadhyay
    School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
    Pharmacol Res 33:67-70. 1996
    CGP 40215A, specific S-adenosylmethionine decarboxylase (AdoMetDC) inhibitor was found to inhibit the growth of Leishmania donovani promastigotes (strain UR6) in a dose-dependent manner with an IC50 of 18 microM...
  15. ncbi S-adenosylmethionine decarboxylase degradation by the 26 S proteasome is accelerated by substrate-mediated transamination
    Azmi Yerlikaya
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    J Biol Chem 279:12469-78. 2004
    The short-lived enzyme S-adenosylmethionine decarboxylase uses a covalently bound pyruvoyl cofactor to catalyze the formation of decarboxylated S-adenosylmethionine, which then donates an aminopropyl group for polyamine biosynthesis...
  16. ncbi Essential role of S-adenosylmethionine decarboxylase in mouse embryonic development
    Kazuhiro Nishimura
    Graduate School of Pharmaceutical Sciences, Chiba University, 1 33, Yayoi cho, Inage Ku, Chiba, 263 8522, Japan
    Genes Cells 7:41-7. 2002
    S-Adenosylmethionine decarboxylase (AdoMetDC) is one of the key enzymes involved in the biosynthesis of spermidine and spermine, which are essential for normal cell growth...
  17. ncbi Half-lives of ornithine decarboxylase and S-adenosylmethionine decarboxylase activities during the cell cycle of Chinese hamster ovary cells
    P S Berntsson
    Department of Animal Physiology, Lund University, Helgonavägen 3 B, Lund, SE 223 62, Sweden
    Biochem Biophys Res Commun 263:13-6. 1999
    ..sampled at various times after cycloheximide addition and the ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) activities were determined...
  18. ncbi BUD2, encoding an S-adenosylmethionine decarboxylase, is required for Arabidopsis growth and development
    Chunmin Ge
    State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    Cell Res 16:446-56. 2006
    ..Our results suggest that polyamines are required for the normal growth and development of higher plants...
  19. pmc Regulation of S-adenosylmethionine decarboxylase activity by alterations in the intracellular polyamine content
    L M Shantz
    Department of Cellular and Molecular Physiology, Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey 17033
    Biochem J 288:511-8. 1992
    ..concentrations, and n-butyl-1,3-diaminopropane, which depletes spermine, on the expression of S-adenosylmethionine decarboxylase (AdoMetDC) activity were studied in mammalian cell lines (HT29, CHO and COS-7)...
  20. pmc Isolation and characterization of Saccharomyces cerevisiae mutants deficient in S-adenosylmethionine decarboxylase, spermidine, and spermine
    M S Cohn
    J Bacteriol 134:208-13. 1978
    Four mutants were isolated from Saccharomyces cerevisiae that are deficient in S-adenosylmethionine decarboxylase (spe2). All four mutants are chromosomal and fall into a single complementation group tightly linked to arg1...
  21. pmc Novel S-adenosylmethionine decarboxylase inhibitors for the treatment of human African trypanosomiasis
    Robert H Barker
    Genzyme Corporation, 153 Second Avenue, Waltham, MA 02451, USA
    Antimicrob Agents Chemother 53:2052-8. 2009
    ..We have begun a program to pursue lead optimization around MDL 73811, an irreversible inhibitor of S-adenosylmethionine decarboxylase (AdoMetDC). This compound is potent but in previous studies cleared rapidly from the blood of rats (T...
  22. pmc Cross-species activation of trypanosome S-adenosylmethionine decarboxylase by the regulatory subunit prozyme
    Erin K Willert
    Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Mol Biochem Parasitol 168:1-6. 2009
    ..Here we show that, as previously demonstrated for T. brucei, S-adenosylmethionine decarboxylase (AdoMetDC) from T...
  23. ncbi Site of pyruvate formation and processing of mammalian S-adenosylmethionine decarboxylase proenzyme
    B A Stanley
    Department of Cellular and Molecular Physiology, Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey 17033
    J Biol Chem 264:21073-9. 1989
    Mammalian S-adenosylmethionine decarboxylase was expressed at a high level in an Escherichia coli mutant deficient in this enzyme...
  24. pmc Co-inhibition of Plasmodium falciparum S-adenosylmethionine decarboxylase/ornithine decarboxylase reveals perturbation-specific compensatory mechanisms by transcriptome, proteome, and metabolome analyses
    Anna C van Brummelen
    Department of Biochemistry, University of Pretoria, Pretoria, Gauteng 0002, South Africa
    J Biol Chem 284:4635-46. 2009
    ..polyamine depletion as an anti-malarial strategy, cytostasis caused by the co-inhibition of S-adenosylmethionine decarboxylase/ornithine decarboxylase in Plasmodium falciparum was studied with a comprehensive transcriptome, ..
  25. doi S-Adenosylmethionine decarboxylase
    Anthony E Pegg
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Essays Biochem 46:25-45. 2009
    S-Adenosylmethionine decarboxylase is a key enzyme for the synthesis of polyamines in mammals, plants and many other species that use aminopropyltransferases for this pathway...
  26. ncbi S-adenosylmethionine decarboxylase from Leishmania donovani. Molecular, genetic, and biochemical characterization of null mutants and overproducers
    Sigrid C Roberts
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, Oregon 97201 3098, USA
    J Biol Chem 277:5902-9. 2002
    The polyamine biosynthetic enzyme, S-adenosylmethionine decarboxylase (ADOMETDC) has been advanced as a potential target for antiparasitic chemotherapy...
  27. doi Transcriptional regulation of the rice arginine decarboxylase (Adc1) and S-adenosylmethionine decarboxylase (Samdc) genes by methyl jasmonate
    Ariadna Peremarti
    Departament de Produccio Vegetal i Ciencia Forestal, ETSEA, Universitat de Lleida, Av Alcalde Rovira Roure 191, E 25198 Lleida, Spain
    Plant Physiol Biochem 48:553-9. 2010
    ..We discuss our findings with respect to the previously proposed threshold model of polyamine metabolism in plants subjected to abiotic stress...
  28. ncbi Structure of a human S-adenosylmethionine decarboxylase self-processing ester intermediate and mechanism of putrescine stimulation of processing as revealed by the H243A mutant
    J L Ekstrom
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853 1301, USA
    Biochemistry 40:9495-504. 2001
    S-Adenosylmethionine decarboxylase (AdoMetDC) is synthesized as a proenzyme that cleaves itself in a putrescine-stimulated reaction via an N-->O acyl shift and beta-elimination to produce an active enzyme with a catalytically essential ..
  29. ncbi The structural basis for substrate specificity and inhibition of human S-adenosylmethionine decarboxylase
    W D Tolbert
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853 1301, USA
    Biochemistry 40:9484-94. 2001
    S-Adenosylmethionine decarboxylase belongs to a small class of amino acid decarboxylases that use a covalently bound pyruvate as a prosthetic group...
  30. ncbi Identification of the primary structure and post-translational modification of rat S-adenosylmethionine decarboxylase
    Makiko Wada
    Faculty of Pharmaceutical Sciences, Josai University, 1 1 Keyakidai, Sakado, Saitama 350 0295, Japan
    Biol Pharm Bull 33:891-4. 2010
    The coding region nucleotide sequences of rat, hamster, and bovine S-adenosylmethionine decarboxylase (AdoMetDC) cDNA exhibit over 90% homology with the human sequence...
  31. pmc In vivo mechanism-based inactivation of S-adenosylmethionine decarboxylases from Escherichia coli, Salmonella typhimurium, and Saccharomyces cerevisiae
    Y F Li
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 98:10578-83. 2001
    S-adenosylmethionine decarboxylase (AdoMetDC), a key enzyme in the biosynthesis of spermidine and spermine, is first synthesized as a proenzyme, which is cleaved posttranslationally to form alpha and beta subunits...
  32. ncbi Polyamine regulation of ribosome pausing at the upstream open reading frame of S-adenosylmethionine decarboxylase
    G L Law
    Department of Biochemistry, University of Washington, Seattle, 98195-7350, USA
    J Biol Chem 276:38036-43. 2001
    Synthesis of S-adenosylmethionine decarboxylase (AdoMetDC), a key regulated enzyme in the pathway of polyamine biosynthesis, is feedback-controlled at the level of translation by spermidine and spermine...
  33. pmc Characterization of monocot and dicot plant S-adenosyl-l-methionine decarboxylase gene families including identification in the mRNA of a highly conserved pair of upstream overlapping open reading frames
    M Franceschetti
    Division of Food Safety Science, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, U K
    Biochem J 353:403-9. 2001
    ....
  34. pmc A phase I study of a new polyamine biosynthesis inhibitor, SAM486A, in cancer patients with solid tumours
    R Paridaens
    Universitair Ziekenhuis Gasthuisberg, Herestraat 49, Leuven, 3000, Belgium
    Br J Cancer 83:594-601. 2000
    ..SAM486A (CGP 48664) is a new inhibitor of the polyamine biosynthetic enzyme S-adenosylmethionine decarboxylase (SAMDC), more potent and specific than the first-generation SAMDC inhibitor methylglyoxal (bis) ..
  35. ncbi Cloning, functional identification and structural modelling of Vitis vinifera S-adenosylmethionine decarboxylase
    Annalisa Tassoni
    Department of Biology e s and Interdepartmental Centre for Biotechnology, University of Bologna, Via Irnerio 42, Bologna, Italy
    J Plant Physiol 164:1208-19. 2007
    In this paper we report the cloning and full sequencing of S-adenosylmethionine decarboxylase (SAMDC, EC 4.1.1.50) cDNA from Vitis vinifera L...
  36. pmc Structural biology of S-adenosylmethionine decarboxylase
    Shridhar Bale
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, 14853, USA
    Amino Acids 38:451-60. 2010
    S-adenosylmethionine decarboxylase (AdoMetDC) is a critical enzyme in the polyamine biosynthetic pathway and a subject of many structural and biochemical investigations for anti-cancer and anti-parasitic therapy...
  37. ncbi A phase I and pharmacokinetic study of SAM486A, a novel polyamine biosynthesis inhibitor, administered on a daily-times-five every-three-week schedule in patients with Advanced solid malignancies
    Lillian L Siu
    Cancer Therapy and Research Center, Institute for Drug Development, San Antonio, Texas 78229, USA
    Clin Cancer Res 8:2157-66. 2002
    SAM486A is a novel inhibitor of the polyamine biosynthetic enzyme S-adenosylmethionine decarboxylase (SAMDC)...
  38. pmc Allosteric regulation of an essential trypanosome polyamine biosynthetic enzyme by a catalytically dead homolog
    Erin K Willert
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Proc Natl Acad Sci U S A 104:8275-80. 2007
    ..S-adenosylmethionine decarboxylase (AdoMetDC) catalyzes a key step in polyamine biosynthesis...
  39. ncbi A dual upstream open reading frame-based autoregulatory circuit controlling polyamine-responsive translation
    Colin Hanfrey
    Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom
    J Biol Chem 280:39229-37. 2005
    A novel form of translational regulation is described for the key polyamine biosynthetic enzyme S-adenosylmethionine decarboxylase (AdoMetDC)...
  40. ncbi Multi-centre Phase II trial of the polyamine synthesis inhibitor SAM486A (CGP48664) in patients with metastatic melanoma
    Michael J Millward
    Sydney Cancer Centre and Sydney Melanoma Unit, Royal Prince Alfred Hospital, Sydney, Australia
    Invest New Drugs 23:253-6. 2005
    To determine the activity and tolerability of SAM496A, an inhibitor of S-adenosylmethionine decarboxylase (SAMDC), in patients with metastatic melanoma who had not received prior chemotherapy...
  41. ncbi Cysteine cathepsins are central contributors of invasion by cultured adenosylmethionine decarboxylase-transformed rodent fibroblasts
    Kirsi Ravanko
    Department of Pathology, Haartman Institute, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland
    Cancer Res 64:8831-8. 2004
    b>Adenosylmethionine decarboxylase (AdoMetDC), a key enzyme in the biosynthesis of polyamines, is often up-regulated in cancers...
  42. ncbi Evolutionary links as revealed by the structure of Thermotoga maritima S-adenosylmethionine decarboxylase
    Angela V Toms
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 279:33837-46. 2004
    S-adenosylmethionine decarboxylase (AdoMetDC) is a critical regulatory enzyme of the polyamine biosynthetic pathway and belongs to a small class of pyruvoyl-dependent amino acid decarboxylases...
  43. ncbi Suppression of S-adenosylmethionine decarboxylase activity is a major cause for high-temperature inhibition of pollen germination and tube growth in tomato (Lycopersicon esculentum Mill.)
    Jianjun Song
    Department of Life Science, Faculty of Bioresources, Mie University, Tsu, Mie, 514 8507 Japan
    Plant Cell Physiol 43:619-27. 2002
    ..Incubation of pollen at 38 degrees C suppressed the increase of S-adenosylmethionine decarboxylase (SAMDC) activity in germinating pollen with little influence on arginine decarboxylase activity...
  44. ncbi Translational regulation of the plant S-adenosylmethionine decarboxylase
    C Hanfrey
    Division of Food Safety Science, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, U K
    Biochem Soc Trans 31:424-7. 2003
    ..S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in polyamine biosynthesis and both mammalian and plant AdoMetDCs are ..
  45. ncbi Mechanism of human S-adenosylmethionine decarboxylase proenzyme processing as revealed by the structure of the S68A mutant
    William D Tolbert
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853 1301, USA
    Biochemistry 42:2386-95. 2003
    S-Adenosylmethionine decarboxylase (AdoMetDC) is a pyruvoyl-dependent enzyme that catalyzes the formation of the aminopropyl group donor in the biosynthesis of the polyamines spermidine and spermine...
  46. ncbi Monomeric S-adenosylmethionine decarboxylase from plants provides an alternative to putrescine stimulation
    Eric M Bennett
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853 1301, USA
    Biochemistry 41:14509-17. 2002
    S-Adenosylmethionine decarboxylase has been implicated in cell growth and differentiation and is synthesized as a proenzyme, which undergoes autocatalytic cleavage to generate an active site pyruvoyl group...
  47. ncbi Putrescine activation of Trypanosoma cruzi S-adenosylmethionine decarboxylase
    Tracy Clyne
    Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9041, USA
    Biochemistry 41:13207-16. 2002
    S-Adenosylmethionine decarboxylase (AdoMetDC) is a pyruvoyl-dependent enzyme that is processed from a single polypeptide into two subunits creating the cofactor...
  48. ncbi Cloning and expression of the S-adenosylmethionine decarboxylase gene of Neurospora crassa and processing of its product
    M A Hoyt
    Department of Molecular Biology and Biochemistry, University of California at Irvine 92697 3900, USA
    Mol Gen Genet 263:664-73. 2000
    S-adenosylmethionine decarboxylase (AdoMetDC) catalyzes the formation of decarboxylated AdoMetDC, a precursor of the polyamines spermidine and spermine. The enzyme is derived from a proenzyme by autocatalytic cleavage...
  49. ncbi Purification of human S-adenosylmethionine decarboxylase expressed in Escherichia coli and use of this protein to investigate the mechanism of inhibition by the irreversible inhibitors, 5'-deoxy-5'-[(3-hydrazinopropyl)methylamino]adenosine and 5'-([(Z)-4-
    L M Shantz
    Department of Cellular and Molecular Physiology, Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey 17033
    Biochemistry 31:6848-55. 1992
    Human S-adenosylmethionine decarboxylase (AdoMetDC) was expressed in high yield in Escherichia coli using the pIN-III(lppP-5) expression vector and purified to apparent homogeneity using affinity chromatography on methylglyoxal bis(..
  50. ncbi The upstream open reading frame of the mRNA encoding S-adenosylmethionine decarboxylase is a polyamine-responsive translational control element
    H Ruan
    Department of Biochemistry, Box 357350, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 271:29576-82. 1996
    S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the pathway of polyamine biosynthesis...
  51. ncbi Cell-specific translational regulation of S-adenosylmethionine decarboxylase mRNA. Dependence on translation and coding capacity of the cis-acting upstream open reading frame
    J R Hill
    Department of Biochemistry, University of Washington, Seattle 98195
    J Biol Chem 268:726-31. 1993
    The mRNA encoding S-adenosylmethionine decarboxylase (AdoMetDC) has a 330-nucleotide 5'-transcript leader containing an open reading frame (uORF) that codes for the hexapeptide MAGDIS...
  52. ncbi The speEspeD operon of Escherichia coli. Formation and processing of a proenzyme form of S-adenosylmethionine decarboxylase
    C W Tabor
    Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892
    J Biol Chem 262:16037-40. 1987
    We have previously shown that the gene (speD) for S-adenosylmethionine decarboxylase is part of an operon that also contains the gene (speE) for spermidine synthase (Tabor, C. W., Tabor, H., and Xie, Q.-W. (1986) Proc. Natl. Acad. Sci. U...
  53. ncbi Cell-specific translation of S-adenosylmethionine decarboxylase mRNA. Regulation by the 5' transcript leader
    J R Hill
    Department of Biochemistry, University of Washington, Seattle 98195
    J Biol Chem 267:21886-93. 1992
    The mRNA encoding S-adenosylmethionine decarboxylase (AdoMetDC) has an unusual distribution in polysomes from cells of T lymphocyte origin...
  54. ncbi The crystal structure of human S-adenosylmethionine decarboxylase at 2.25 A resolution reveals a novel fold
    J L Ekstrom
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
    Structure 7:583-95. 1999
    S-Adenosylmethionine decarboxylase (AdoMetDC) is a critical regulatory enzyme of the polyamine synthetic pathway, and a well-studied drug target...
  55. ncbi Amino acid residues necessary for putrescine stimulation of human S-adenosylmethionine decarboxylase proenzyme processing and catalytic activity
    B A Stanley
    Department of Cellular and Molecular Physiology, Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey 17033
    J Biol Chem 266:18502-6. 1991
    ....
  56. ncbi Irreversible inhibition of rat S-adenosylmethionine decarboxylase by 5'-([(Z)-4-amino-2-butenyl]methylamino)-5'-deoxyadenosine
    C Danzin
    Merrell Dow Research Institute, Strasbourg Research Centre, France
    Biochem Pharmacol 40:1499-503. 1990
    ..In rats, intraperitoneal administration of (Z)-AbeAdo produces a rapid, long-lasting and dose-dependent decrease of AdoMetDC activity in ventral prostate, testis and brain...
  57. ncbi Abrogation of upstream open reading frame-mediated translational control of a plant S-adenosylmethionine decarboxylase results in polyamine disruption and growth perturbations
    Colin Hanfrey
    Division of Food Safety Science, Institute of Food Research, Norwich Research Park, Colney, United Kingdom
    J Biol Chem 277:44131-9. 2002
    S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in polyamine biosynthesis. We show that the plant AdoMetDC activity is subject to post-transcriptional control by polyamines...
  58. ncbi Regulated translation termination at the upstream open reading frame in s-adenosylmethionine decarboxylase mRNA
    Alexa Raney
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
    J Biol Chem 277:5988-94. 2002
    The upstream open reading frame (uORF) in the mRNA encoding S-adenosylmethionine decarboxylase is a cis-acting element that confers feedback control by cellular polyamines on translation of this message...
  59. ncbi Inhibition of multidrug-resistant HIV-1 by interference with cellular S-adenosylmethionine decarboxylase activity
    Birgit Schäfer
    Heinrich Pette Institute for Experimental Virology and Immunology, Martinistrasse, Hamburg, Germany
    J Infect Dis 194:740-50. 2006
    S-adenosylmethionine decarboxylase (SAMDC), a key enzyme in polyamine biosynthesis, can be specifically inhibited by the experimental drug SAM486A...
  60. ncbi In the human malaria parasite Plasmodium falciparum, polyamines are synthesized by a bifunctional ornithine decarboxylase, S-adenosylmethionine decarboxylase
    S Muller
    Bernhard Nocht Institute for Tropical Medicine, Biochemical Parasitology, Bernhard Nocht Str 74, D 20359 Hamburg, Germany
    J Biol Chem 275:8097-102. 2000
    ..polyamine biosynthesis by inhibition of the rate-limiting enzymes ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) has been discussed as a potential chemotherapy of cancer and parasitic infections...
  61. pmc The pivotal roles of the plant S-adenosylmethionine decarboxylase 5' untranslated leader sequence in regulation of gene expression at the transcriptional and posttranscriptional levels
    Wen Wei Hu
    Plant Genetic Engineering Laboratory, Department of Biological Sciences, National University of Singapore, Singapore 117543
    Plant Physiol 138:276-86. 2005
    S-Adenosylmethionine decarboxylase (SAMDC; EC 4.1.1.50) is a key rate-limiting enzyme located in the polyamine biosynthesis pathway...
  62. ncbi S-adenosylmethionine decarboxylase overexpression reduces invasiveness and tumorigenicity in nude mice of MCF-7 breast cancer cells
    A Manni
    Division of Endocrinology, H044 Hershey Medical Center, 500 University Drive, Hershey, PA 17033 0850, USA
    Int J Oncol 19:317-23. 2001
    To elucidate the role of S-adenosylmethionine decarboxylase (SAMDC) in breast cancer biology, we have generated SAMDC overexpressing MCF-7 breast cancer cells...
  63. ncbi Overexpression of S-adenosylmethionine decarboxylase (SAMDC) in Xenopus embryos activates maternal program of apoptosis as a "fail-safe" mechanism of early embryogenesis
    Masatake Kai
    Laboratory of Molecular Embryology, Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 0033, Japan
    Cell Res 13:147-58. 2003
    In Xenopus, injection of S-adenosylmethionine decarboxylase (SAMDC) mRNA into fertilized eggs or 2-cell stage embryos induces massive cell dissociation and embryo-lysis at the early gastrula stage due to activation of the maternal program ..
  64. ncbi Maternal program of apoptosis activated shortly after midblastula transition by overexpression of S-adenosylmethionine decarboxylase in Xenopus early embryos
    K Shiokawa
    Laboratory of Molecular Embryology, Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan
    Comp Biochem Physiol B Biochem Mol Biol 126:149-55. 2000
    When we studied polyamine metabolism in Xenopus embryos, we cloned the cDNA for Xenopus S-adenosylmethionine decarboxylase (SAMDC), which converts SAM (S-adenosylmethionine), the methyl donor, into decarboxylated SAM (dcSAM), the ..
  65. pmc S-Adenosylmethionine decarboxylase from the archaeon Methanococcus jannaschii: identification of a novel family of pyruvoyl enzymes
    A D Kim
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Bacteriol 182:6667-72. 2000
    ..Both SpeD-type and archaeal-type enzymes have diverged widely in sequence and size from analogous eucaryal enzymes...
  66. ncbi Occurrence of pre-MBT synthesis of caspase-8 mRNA and activation of caspase-8 prior to execution of SAMDC (S-adenosylmethionine decarboxylase)-induced, but not p53-induced, apoptosis in Xenopus late blastulae
    Koichiro Shiokawa
    Laboratory of Molecular Embryology, Department of Biological Science, Graduate School of Sciences, The University of Tokyo, 7 3 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Biochem Biophys Res Commun 336:682-91. 2005
    Overexpression of S-adenosylmethionine decarboxylase (SAMDC) in Xenopus fertilized eggs activates caspase-9 and executes maternal program of apoptosis shortly after midblastula transition (MBT)...
  67. ncbi Mechanisms of allosteric regulation of Trypanosoma cruzi S-adenosylmethionine decarboxylase
    Tracy Clyne Beswick
    Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 6001 Forest Park Boulevard, Dallas, Texas 75390 9041, USA
    Biochemistry 45:7797-807. 2006
    S-Adenosylmethionine decarboxylase (AdoMetDC) is a pyruvoyl-dependent enzyme that catalyzes an essential step in polyamine biosynthesis...
  68. ncbi Structure and regulation of mammalian S-adenosylmethionine decarboxylase
    A Pajunen
    Department of Biochemistry, University of Oulu, Finland
    J Biol Chem 263:17040-9. 1988
    In order to understand the structure and regulation of S-adenosylmethionine decarboxylase, cDNA clones encoding this enzyme have been isolated from rat prostate and human fibroblast cDNA libraries...
  69. pmc In vivo trypanocidal activities of new S-adenosylmethionine decarboxylase inhibitors
    C J Bacchi
    Haskins Laboratories, Pace University, New York, New York 10038, USA
    Antimicrob Agents Chemother 40:1448-53. 1996
    ..MGBG has modest activity as an inhibitor of trypanosome S-adenosylmethionine decarboxylase (50% inhibitory concentration [IC50]...
  70. ncbi Overexpression of carnation S-adenosylmethionine decarboxylase gene generates a broad-spectrum tolerance to abiotic stresses in transgenic tobacco plants
    Soo Jin Wi
    Department of Biology, College of Natural Science, Sunchon National University, Sunchon, Chonnam 540 742, Korea
    Plant Cell Rep 25:1111-21. 2006
    ..Sense construct of full-length cDNA for S-adenosylmethionine decarboxylase (SAMDC), a key enzyme in PA biosynthesis, from carnation (Dianthus caryophyllus L...
  71. ncbi Adenovirus-mediated expression of both antisense ODC and AdoMetDC inhibited colorectal cancer cell growth in vitro
    Bing Zhang
    Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China
    Acta Pharmacol Sin 27:353-9. 2006
    ..adenovirus that can simultaneously express both antisense ornithine decarboxylase (ODC) and adenosylmethionine decarboxylase ( AdoMetDC ) and detect its inhibitory effect on the intracellular polyamine pool and colorectal ..
  72. ncbi Role of polyamines in peach fruit development and storage
    Jihong Liu
    Department of Plant, Cell and Environment, National Institute of Fruit Tree Science, Tsukuba, Ibaraki 305 8605, Japan
    Tree Physiol 26:791-8. 2006
    ..Activity of S-adenosylmethionine decarboxylase (SAMDC) decreased progressively until the end of S2...
  73. ncbi Mutants of Saccharomyces cerevisiae deficient in polyamine biosynthesis: studies on the regulation of ornithine decarboxylase
    C W Tabor
    Med Biol 59:272-8. 1981
    ..decarboxylase activity and therefore cannot make putrescine; (ii) spe2 mutants, which have no adenosylmethionine decarboxylase and therefore cannot make spermidine or spermine; (iii) spe3 mutants, which have no putrescine ..
  74. ncbi Polyamine depletion by ODC-AdoMetDC antisense adenovirus impairs human colorectal cancer growth and invasion in vitro and in vivo
    Bing Zhang
    Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China
    J Gene Med 8:980-9. 2006
    Polyamine biosynthesis is controlled primarily by ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC). Polyamine concentrations are elevated in colorectal cancer...
  75. ncbi Mass screening for mutants in the biosynthetic pathway for polyamines in Escherichia coli: a general method for mutants in enzymatic reactions producing CO2
    C W Tabor
    Methods Enzymol 94:83-91. 1983
  76. ncbi Inhibition of enzymes of polyamine biosynthesis by substrate-like O-substituted hydroxylamines
    A R Khomutov
    Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia
    Biochemistry (Mosc) 67:1159-67. 2002
    ..To inhibit pyridoxal-5'-phosphate dependent ornithine decarboxylase and pyruvate dependent S-adenosylmethionine decarboxylase, key enzymes of polyamine biosynthesis, a system of substrate-like O-substituted hydroxylamines is ..
  77. ncbi Resveratrol-induced modification of polyamine metabolism is accompanied by induction of c-Fos
    Freya Wolter
    Second Department of Medicine, J W Goethe University, 60590 Frankfurt Main, Germany
    Carcinogenesis 24:469-74. 2003
    ..S-Adenosylmethionine decarboxylase, an enzyme that synthesizes higher polyamines, was concomitantly inhibited by resveratrol and ..
  78. ncbi The putrescine analogue 1-aminooxy-3-aminopropane perturbs polyamine metabolism in the phytopathogenic fungus Sclerotinia sclerotiorum
    Andrés Gárriz
    Instituto de Investigaciones Biotecnológicas Instituto Tecnológico de Chascomús, Universidad Nacional de General San Martín Consejo Nacional de Investigaciones Científicas y Técnicas, Casilla de Correo 164, 7130, Chascomús, Buenos Aires, Argentina
    Arch Microbiol 180:169-75. 2003
    ..The potential of 1-aminooxy-3-aminopropane as a tool for studies on fungal polyamine metabolism and for the control of plant diseases of fungal origin is discussed...
  79. ncbi Effects of spermidine synthase overexpression on polyamine biosynthetic pathway in tobacco plants
    Marina Franceschetti
    Department of Biology e s and Interdepartmental Centre of Biotechnology, University of Bologna, Via Irnerio 42, Bologna 40126, Italy
    J Plant Physiol 161:989-1001. 2004
    ..No relationship was detected between the mRNA expression level of S-adenosylmethionine decarboxylase (SAMDC, EC 4.1.1...
  80. ncbi Dietary putrescine reduces the intestinal anticarcinogenic activity of sulindac in a murine model of familial adenomatous polyposis
    Natalia A Ignatenko
    Department of Cell Biology and Anatomy, Arizona Cancer Center, The University of Arizona, Tucson, USA
    Nutr Cancer 56:172-81. 2006
    ..Sulindac also decreased the activity of the biosynthetic enzyme ornithine decarboxylase but not adenosylmethionine decarboxylase (AMD)...
  81. pmc The presence of an active S-adenosylmethionine decarboxylase gene increases the growth defect observed in Saccharomyces cerevisiae mutants unable to synthesize putrescine, spermidine, and spermine
    D Balasundaram
    Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892
    J Bacteriol 176:6407-9. 1994
    ..decarboxylase) and spe1 delta spe2 delta mutants (lacking both ornithine decarboxylase and S-adenosylmethionine decarboxylase) are equally unable to synthesize putrescine, spermidine, and spermine and require spermidine or ..
  82. ncbi Methotrexate differentially affects growth of suspension and adherent cells
    Elza Kimura
    Graduate School of Pharmaceutical Sciences, Chiba University, 1 33 Yayoi cho, Ingae ku, Chiba 263 8522, Japan
    Int J Biochem Cell Biol 36:814-25. 2004
    ..This may be relevant to the in vivo effects of low doses of methotrexate, which have immunosuppressive and anti-inflammatory effects, because lymphocytes are suspension cells...
  83. ncbi Key role for p27Kip1, retinoblastoma protein Rb, and MYCN in polyamine inhibitor-induced G1 cell cycle arrest in MYCN-amplified human neuroblastoma cells
    Christopher J Wallick
    Cancer Research Center of Hawaii, University of Hawaii at Manoa, 1236 Lauhala Street, Honolulu, HI 96813, USA
    Oncogene 24:5606-18. 2005
    ..Treatment with DFMO in combination with SAM486A, an S-adenosylmethionine decarboxylase (AdoMetDC) inhibitor, has been shown to enhance polyamine pool depletion...
  84. ncbi Polyamine-iron chelator conjugate
    Raymond J Bergeron
    Department of Medicinal Chemistry, University of Florida, Gainesville, Florida 32610 0485, USA
    J Med Chem 46:5478-83. 2003
    ..ligand, decreases the activities of the polyamine biosynthetic enzymes ornithine decarboxylase and S-adenosylmethionine decarboxylase, and upregulates spermidine-spermine N (1)-acetyltransferase...
  85. ncbi Peach (Prunus persica L.) fruit growth and ripening: transcript levels and activity of polyamine biosynthetic enzymes in the mesocarp
    Vanina Ziosi
    Dipartimento di Biologia E S, University of Bologna, Via Irnerio 42, I 40126 Bologna, Italy
    J Plant Physiol 160:1109-15. 2003
    ..1.1.19), ornithine decarboxylase (ODC, EC 4.1.1.17) and S-adenosylmethionine decarboxylase (SAMDC, EC 4.1.1...
  86. ncbi Thirty years of polyamine-related approaches to cancer therapy. Retrospect and prospect. Part 1. Selective enzyme inhibitors
    Nikolaus Seiler
    Laboratory of Nutritional Oncology, INSERM U 392, Institut de Recherche contre les Cancers de l Appareil Digestif, 1, Place de l Hopital, B P 426, 67091 Strasbourg, France
    Curr Drug Targets 4:537-64. 2003
    ..The high activities of ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) in rapidly growing tissues and cells, particularly in tumour cells, suggested PA ..
  87. pmc Overproduction of cardiac S-adenosylmethionine decarboxylase in transgenic mice
    Oleg Nisenberg
    Department of Cellular and Molecular Physiology, The Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, P O Box 850, 500 University Drive, Hershey, PA 17033, USA
    Biochem J 393:295-302. 2006
    The present study was designed to provide a better understanding of the role played by AdoMetDC (S-adenosylmethionine decarboxylase), the key rate-controlling enzyme in the synthesis of spermidine and spermine, in controlling polyamine ..
  88. ncbi Functional GATA- and initiator-like-elements exhibit a similar arrangement in the promoters of Caenorhabditis elegans polyamine synthesis enzymes
    Kai Lüersen
    Department of Biochemistry, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str 74, D 20359 Hamburg, Germany
    Biol Chem 385:711-21. 2004
    ..elegans the polyamine synthetic pathway consists of three enzymes, ornithine decarboxylase (ODC), S-adenosylmethionine decarboxylase (AdoMetDC) and spermidine synthase. Their gene expression pattern was determined in C...
  89. ncbi Inhibition of the ethylene response by 1-MCP in tomato suggests that polyamines are not involved in delaying ripening, but may moderate the rate of ripening or over-ripening
    Annalisa Tassoni
    Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA
    J Exp Bot 57:3313-25. 2006
    ..No effect of treatment on ornithine decarboxylase activity was detected. Polyamines are thus not directly associated with a delay in tomato fruit ripening, but may prolong the fully-ripe stage before the fruit tissues undergo senescence...
  90. pmc Leishmania donovani polyamine biosynthetic enzyme overproducers as tools to investigate the mode of action of cytotoxic polyamine analogs
    Sigrid C Roberts
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97239 3098, USA
    Antimicrob Agents Chemother 51:438-45. 2007
    ..Promastigotes overexpressing episomal constructs encoding ornithine decarboxylase (ODC), S-adenosylmethionine decarboxylase (ADOMETDC), or spermidine synthase (SPDSYN) revealed robust overproduction of the corresponding ..
  91. ncbi Activation of caspase-3 activity and apoptosis in MDA-MB-468 cells by N(omega)-hydroxy-L-arginine, an inhibitor of arginase, is not solely dependent on reduction in intracellular polyamines
    R Singh
    Department of Obstetrics, Jonsson Comprehensive Cancer Center, University of California School of Medicine, Los Angeles, CA 90095-1740, USA
    Carcinogenesis 22:1863-9. 2001
    ..possibility, we used SAM-486A and alpha-difluoromethylornithine (DFMO), which are inhibitors of S-adenosylmethionine decarboxylase (SAMDC), and ornithine decarboxylase (ODC), respectively, either alone or in combination to reduce ..
  92. ncbi Regulation of ornithine decarboxylase in the kidney of autoimmune mice with the lpr gene
    H C Hsu
    Program in Clinical Pharmacology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, New Brunswick 08903, USA
    Autoimmunity 19:253-64. 1994
    ..The link between aberrant regulation of ODC and the immunopathogenesis of murine lupus nephritis indicates novel targets for lupus therapy...
  93. ncbi The drought response of Theobroma cacao (cacao) and the regulation of genes involved in polyamine biosynthesis by drought and other stresses
    Hanhong Bae
    US Department of Agriculture Agricultural Research Service, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA
    Plant Physiol Biochem 46:174-88. 2008
    ..were determined: an ornithine decarboxylase (TcODC), an arginine decarboxylase (TcADC), an S-adenosylmethionine decarboxylase (TcSAMDC), a spermidine synthase (TcSPDS), and a spermine synthase (TcSPMS)...
  94. ncbi [Effects of salt stress on cucumber seedlings root growth and polyamine metabolism]
    Jiu Ju Duan
    College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
    Ying Yong Sheng Tai Xue Bao 19:57-64. 2008
    ..NaCl stress caused an increase of arginine decarboxylase (ADC), ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (SAMDC) activities in cucumber roots, which was much greater in 'Changchun mici' than in 'Jinchun No...
  95. ncbi Tumor progression is accompanied by significant changes in the levels of expression of polyamine metabolism regulatory genes and clusterin (sulfated glycoprotein 2) in human prostate cancer specimens
    S Bettuzzi
    Department of Scienze Biomediche, Universita di Modena e Reggio Emilia, Modena, Italy
    Cancer Res 60:28-34. 2000
    ..The results indicate that transcripts encoding ornithine decarboxylase (ODC), ODC antizyme, adenosylmethionine decarboxylase, and spermidine/spermine N1-acetyltransferase (SSAT) were significantly higher, whereas clusterin (..
  96. doi Assessing the polyamine metabolism of Plasmodium falciparum as chemotherapeutic target
    Ingrid B Müller
    Bernhard Nocht Institute for Tropical Medicine, Department of Biochemistry, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany
    Mol Biochem Parasitol 160:1-7. 2008
    ..has its own and unique polyamine synthesis, differing highly from the mammalian one mainly in the arrangement of the key enzymes, S-adenosylmethionine decarboxylase and ornithine decarboxylase (AdoMetDC/ODC), on a bifunctional protein.
  97. doi Effects of antisense RNA targeting of ODC and AdoMetDC on the synthesis of polyamine synthesis and cell growth in prostate cancer cells using a prostatic androgen-dependent promoter in adenovirus
    Wei Li
    Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan, Shandong, China
    Prostate 68:1354-61. 2008
    ..androgen-dependent promoter to mediate antisense targeting of ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) and its effects on the synthesis of polyamine...
  98. pmc Crenarchaeal arginine decarboxylase evolved from an S-adenosylmethionine decarboxylase enzyme
    Teresa N Giles
    Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712, USA
    J Biol Chem 283:25829-38. 2008
    ..Instead they have two paralogs of the S-adenosylmethionine decarboxylase (AdoMetDC)...
  99. ncbi The Plasmodium falciparum bifunctional ornithine decarboxylase, S-adenosyl-L-methionine decarboxylase, enables a well balanced polyamine synthesis without domain-domain interaction
    C Wrenger
    Bernhard Nocht Institute for Tropical Medicine, Biochemical Parasitology, Bernhard-Nocht-Strasse 74, D-20359 Hamburg, Germany
    J Biol Chem 276:29651-6. 2001
    ..These results demonstrate that no domain-domain interactions occur between the two enzymes of the bifunctional PfODC/AdoMetDC and that both enzymatic activities are operating as independent catalytic sites that do not affect each other...
  100. ncbi Antizyme regulates the degradation of ornithine decarboxylase in fission yeast Schizosaccharomyces pombe. Study in the spe2 knockout strains
    M K Chattopadhyay
    Department of Biochemistry II, Jikei University School of Medicine, 3-25-8, Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan
    J Biol Chem 276:21235-41. 2001
    ..To regulate cellular spermidine experimentally, we cloned and disrupted S-adenosylmethionine decarboxylase gene (spe2) in S. pombe...
  101. ncbi Developmental expression of ornithine and S-adenosylmethionine decarboxylases in mouse brain
    A Suorsa
    Biocenter, University of Oulu, Finland
    Biochem Biophys Res Commun 184:1114-8. 1992
    ..of the two key enzymes in mammalian polyamine synthesis, ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) in mouse brain show distinct, but inverse, changes during ontogeny...

Research Grants68

  1. Polyamine Metabolism in Leishmania
    Buddy Ullman; Fiscal Year: 2006
    ..The proposal will focus on arginase (ARG), agmatinase (AGM), ornithine decarboxylase (ODC), S-adenosylmethionine decarboxylase (ADOMETDC), and spermidine synthase (SYN), the entire complement of polyamine and polyamine precursor ..
  2. TRYPANOSMIASIS--MOLECULAR APPROACHES TO CHEMOTHERAPY
    Margaret Phillips; Fiscal Year: 2003
    ..We have identified three drug targets, ornithine decarboxylase (ODC), S-adenosylmethionine decarboxylase (SAMDC) and gamma-glutamylcysteine synthetase (gammaGCS), which are essential for the biosynthesis of ..
  3. Role of Polyamines in MYCN-Amplified Neuroblastoma
    ANDRE BACHMANN; Fiscal Year: 2009
    ..which specifically inhibit the polyamine biosynthetic enzymes ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC), respectively...
  4. Role of Polyamines in MYCN-Amplified Neuroblastoma
    ANDRE BACHMANN; Fiscal Year: 2009
    ..which specifically inhibit the polyamine biosynthetic enzymes ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC), respectively...
  5. Role of Polyamines in MYCN-Amplified Neuroblastoma
    ANDRE BACHMANN; Fiscal Year: 2007
    ..which specifically inhibit the polyamine biosynthetic enzymes ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC), respectively...
  6. Targeting S-adenosylmethionine decarboxylase for HAT drug discovery
    Margaret A Phillips; Fiscal Year: 2010
    ..The polyamine biosynthetic enzymes, including ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) are essential to the parasite...
  7. Polyamine and glutathione metabolism in trypanosomes.
    Margaret Phillips; Fiscal Year: 2007
    ..In this proposal our planned studies focus on the three enzymes in the pathway (ODC, S-adenosylmethionine decarboxylase and gamma-glutamylcysteine synthetase) that catalyze the first committed steps in the biosynthesis of ..
  8. Rational Design of Inhibitors of Polyamine Synthesis
    Steven Ealick; Fiscal Year: 2007
    ..In the proposed research, we will focus on S-adenosylmethionine decarboxylase (AdoMetDC), spermine/spermidine N1-acetyl transferase (SSAT) and the polyamine synthases, spermidine ..
  9. POLYAMINE METABOLISM IN TRYPANOSOMATIDS
    Buddy Ullman; Fiscal Year: 2001
    ..This application will focus on the ornithine decarboxylase (ODC), S-adenosylmethionine decarboxylase (ADOMETDC), and spermidine synthase (SPDSYN) proteins from Leishmania donovani and the ADOMETDC from ..
  10. GROWTH REGULATION OF POLYAMINE SYNTHESIS
    David Morris; Fiscal Year: 2001
    S-adenosylmethionine decarboxylase (AdoMetDC) is a key regulated enzyme in the pathway of synthesis of the polyamines, spermidine and spermine...
  11. STRUCTURAL STUDIES OF METHYL CYCLE ENZYMES
    Fusao Takusagawa; Fiscal Year: 2003
    ..S-adenosylhomocysteine hydrolase from rat liver and Alcaligenes faecalis. S-adenosylmethionine decarboxylase from E. coli. N10-formyl-tetrahydrofolate synthetase from Clostridium cylindrosporum...
  12. Folate, Vitamin D and Calcium in Colorectal Cancer
    Robert Haile; Fiscal Year: 2009
    ..adenosyl transferase 2A (MAT2A), folate receptor (FOLR1), reduced folate carrier (RFC1 or SLC19A1), S-adenosylmethionine decarboxylase (AMD1), gastric instrinsic factor (GIF), transcobalamin II (TCII), intrinsic factor cobalamin ..
  13. Folate, Vitamin D and Calcium in Colorectal Cancer
    Robert Haile; Fiscal Year: 2007
    ..adenosyl transferase 2A (MAT2A), folate receptor (FOLR1), reduced folate carrier (RFC1 or SLC19A1), S-adenosylmethionine decarboxylase (AMD1), gastric instrinsic factor (GIF), transcobalamin II (TCII), intrinsic factor cobalamin ..