carbon carbon lyases

Summary

Summary: Enzymes that catalyze the cleavage of a carbon-carbon bond by means other than hydrolysis or oxidation. This subclass contains the DECARBOXYLASES, the ALDEHYDE-LYASES, and the OXO-ACID-LYASES. EC 4.1.

Top Publications

  1. ncbi Physical organization and phylogenetic analysis of acdR as leucine-responsive regulator of the 1-aminocyclopropane-1-carboxylate deaminase gene acdS in phytobeneficial Azospirillum lipoferum 4B and other Proteobacteria
    Claire Prigent-Combaret
    Universite de Lyon, Lyon, F 69003, France
    FEMS Microbiol Ecol 65:202-19. 2008
  2. pmc Evolving biosynthetic tangos negotiate mechanistic landscapes
    Michael B Austin
    The Salk Institute for Biological Studies, Jack H Skirball Center for Chemical Biology and Proteomics, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Chem Biol 4:217-22. 2008
  3. ncbi A single-vial analytical and quantitative gas chromatography-mass spectrometry assay for terpene synthases
    PAUL E O'MAILLE
    Structural Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Anal Biochem 335:210-7. 2004
  4. ncbi Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase
    Matthias Brock
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
    Eur J Biochem 269:6184-94. 2002
  5. ncbi Biochemical and genetic evidence of benzylsuccinate synthase in toluene-degrading, ferric iron-reducing Geobacter metallireducens
    Staci R Kane
    Lawrence Livermore National Laboratory, Livermore, CA 94551 0808, USA
    Biodegradation 13:149-54. 2002
  6. ncbi Response of spring rape (Brassica napus var. oleifera L.) to inoculation with plant growth promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase depends on nutrient status of the plant
    Andrei A Belimov
    Laboratory of Ecology of Symbiotic and Associative Bacteria, All Russia Research Institute for Agricultural Microbiology, Saint Petersburg
    Can J Microbiol 48:189-99. 2002
  7. ncbi Structural and enzymatic properties of 1-aminocyclopropane-1-carboxylate deaminase homologue from Pyrococcus horikoshii
    Aiko Fujino
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan
    J Mol Biol 341:999-1013. 2004
  8. ncbi Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-beneficial properties
    A Sessitsch
    ARC Seibersdorf Research GmbH, Department of Bioresources Microbiology, Austria
    Int J Syst Evol Microbiol 55:1187-92. 2005
  9. pmc Depth-resolved quantification of anaerobic toluene degraders and aquifer microbial community patterns in distinct redox zones of a tar oil contaminant plume
    Christian Winderl
    Institute of Groundwater Ecology, Helmholtz Center Munich, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764 Neuherberg, Germany
    Appl Environ Microbiol 74:792-801. 2008
  10. ncbi The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds
    Vitaly Portnoy
    Institute of Plant Sciences, Newe Ya ar Research Center, Agricultural Research Organization, P O Box 1021, Ramat Yishay 30095, Israel
    Plant Mol Biol 66:647-61. 2008

Research Grants

  1. ENZYME CATALYSIS OF ELECTRON AND GROUP TRANSFER
    PERRY FREY; Fiscal Year: 2006
  2. MECHANISMS OF ENZYMATIC REACTIONS
    PERRY FREY; Fiscal Year: 2006

Scientific Experts

Detail Information

Publications142 found, 100 shown here

  1. ncbi Physical organization and phylogenetic analysis of acdR as leucine-responsive regulator of the 1-aminocyclopropane-1-carboxylate deaminase gene acdS in phytobeneficial Azospirillum lipoferum 4B and other Proteobacteria
    Claire Prigent-Combaret
    Universite de Lyon, Lyon, F 69003, France
    FEMS Microbiol Ecol 65:202-19. 2008
    ..lipoferum 4B. This is the first synteny, phylogenetic, and functional analysis of factors modulating acdS expression in Azospirillum plant growth-promoting rhizobacterium...
  2. pmc Evolving biosynthetic tangos negotiate mechanistic landscapes
    Michael B Austin
    The Salk Institute for Biological Studies, Jack H Skirball Center for Chemical Biology and Proteomics, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Chem Biol 4:217-22. 2008
    ..Evolution of these enzyme families relies on rhythmic tangos, in which the enzymes and substrates together determine product outcome by negotiating decision networks governing intrinsic and induced chemical reactivities...
  3. ncbi A single-vial analytical and quantitative gas chromatography-mass spectrometry assay for terpene synthases
    PAUL E O'MAILLE
    Structural Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Anal Biochem 335:210-7. 2004
    ....
  4. ncbi Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase
    Matthias Brock
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
    Eur J Biochem 269:6184-94. 2002
    ..This is in good agreement with investigations on Salmonella enterica LT2, in which disruption of the prpE gene showed no visible phenotype...
  5. ncbi Biochemical and genetic evidence of benzylsuccinate synthase in toluene-degrading, ferric iron-reducing Geobacter metallireducens
    Staci R Kane
    Lawrence Livermore National Laboratory, Livermore, CA 94551 0808, USA
    Biodegradation 13:149-54. 2002
    ....
  6. ncbi Response of spring rape (Brassica napus var. oleifera L.) to inoculation with plant growth promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase depends on nutrient status of the plant
    Andrei A Belimov
    Laboratory of Ecology of Symbiotic and Associative Bacteria, All Russia Research Institute for Agricultural Microbiology, Saint Petersburg
    Can J Microbiol 48:189-99. 2002
    ..The results obtained show that the growth-promoting effects of ACC-utilizing rhizobacteria depend significantly on the nutrient status of the plant...
  7. ncbi Structural and enzymatic properties of 1-aminocyclopropane-1-carboxylate deaminase homologue from Pyrococcus horikoshii
    Aiko Fujino
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan
    J Mol Biol 341:999-1013. 2004
    ..We also propose a condition necessary in the primary sequence to have ACCD activity...
  8. ncbi Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-beneficial properties
    A Sessitsch
    ARC Seibersdorf Research GmbH, Department of Bioresources Microbiology, Austria
    Int J Syst Evol Microbiol 55:1187-92. 2005
    ..nov. is proposed. The type strain is strain PsJN(T) (=LMG 22146(T) = CCUG 49060(T))...
  9. pmc Depth-resolved quantification of anaerobic toluene degraders and aquifer microbial community patterns in distinct redox zones of a tar oil contaminant plume
    Christian Winderl
    Institute of Groundwater Ecology, Helmholtz Center Munich, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764 Neuherberg, Germany
    Appl Environ Microbiol 74:792-801. 2008
    ....
  10. ncbi The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds
    Vitaly Portnoy
    Institute of Plant Sciences, Newe Ya ar Research Center, Agricultural Research Organization, P O Box 1021, Ramat Yishay 30095, Israel
    Plant Mol Biol 66:647-61. 2008
    ..The genes are differentially transcriptionally regulated during fruit development and according to variety and are likely to be associated with chemical differences responsible for the unique aromas of melon varieties...
  11. ncbi Reaction of 1-amino-2-methylenecyclopropane-1-carboxylate with 1-aminocyclopropane-1-carboxylate deaminase: analysis and mechanistic implications
    Zongbao Zhao
    Division of Medicinal Chemistry, College of Pharmacy, University of Texas, Austin, Texas 78712, USA
    Biochemistry 42:2089-103. 2003
    ..Clearly, the cleavage of ACC to alpha-ketobutyrate by ACC deaminase represents an intriguing conversion beyond the common scope entailed by coenzyme B(6) dependent catalysts...
  12. ncbi Phytanic acid alpha-oxidation, new insights into an old problem: a review
    Ronald J A Wanders
    Laboratory Genetic Metabolic Diseases, Department of Pediatrics Emma Children s Hospital and Clinical Chemistry, Academic Medical Centre, University Hospital Amsterdam, Room F0 224, P O Box 22700, 1100 DE Amsterdam, The Netherlands
    Biochim Biophys Acta 1631:119-35. 2003
    ..This Review describes the current state of knowledge about fatty acid alpha-oxidation in mammals with particular emphasis on the mechanism involved and the enzymology of the pathway...
  13. ncbi Crystal structure of 2-methylisocitrate lyase (PrpB) from Escherichia coli and modelling of its ligand bound active centre
    Clemens Grimm
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    J Mol Biol 328:609-21. 2003
    ....
  14. ncbi Expression and characterization of 1-aminocyclopropane-1-carboxylate deaminase from the rhizobacterium Pseudomonas putida UW4: a key enzyme in bacterial plant growth promotion
    Nikos Hontzeas
    Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada N2L 3G1
    Biochim Biophys Acta 1703:11-9. 2004
    ..This enzyme's biochemical and biophysical properties will serve as an important reference point to which newly isolated ACC deaminases from other organisms can be compared...
  15. ncbi Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries
    Joost Lücker
    Biotechnology Laboratory, Department of Botany, University of British Columbia, 6174 University Boulevard, Vancouver, BC, V6T 1Z3, Canada
    Phytochemistry 65:2649-59. 2004
    ..Sesquiterpene synthase transcripts were also detected in young seeds...
  16. ncbi Carboxymethylproline synthase (CarB), an unusual carbon-carbon bond-forming enzyme of the crotonase superfamily involved in carbapenem biosynthesis
    Mark C Sleeman
    Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, UK
    J Biol Chem 279:6730-6. 2004
    ..The implications of the results for the biosynthesis of both carbapenem-3-carboxylate and C-2/C-6-substituted carbapenems, such as thienamycin, are discussed...
  17. ncbi MosA, a protein implicated in rhizopine biosynthesis in Sinorhizobium meliloti L5-30, is a dihydrodipicolinate synthase
    Pui Hang Tam
    Department of Biochemistry, University of Saskatchewan, 110 Science Place, Saskatoon, Sask, Canada S7N 5C9
    J Mol Biol 335:393-7. 2004
    ..A role for this enzyme in rhizopine synthesis cannot be ruled out, but is called into question...
  18. ncbi Evolution of structure and function in the o-succinylbenzoate synthase/N-acylamino acid racemase family of the enolase superfamily
    Margaret E Glasner
    Department of Biopharmaceutical Sciences, University of California, San Francisco, CA 94143, USA
    J Mol Biol 360:228-50. 2006
    ..Finally, a combination of evolutionary, structural, and sequence analyses identified characteristics that might prime proteins, such as Amycolatopsis OSBS/NAAAR, for the evolution of new activities...
  19. pmc Aerobic and anaerobic toluene degradation by a newly isolated denitrifying bacterium, Thauera sp. strain DNT-1
    Yoshifumi Shinoda
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Appl Environ Microbiol 70:1385-92. 2004
    ..On the basis of these results, it is concluded that strain DNT-1 modulates the expression of two different initial pathways of toluene degradation according to the availability of oxygen in the environment...
  20. ncbi Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum
    Barbara Morasch
    Fakultat fur Biologie, Universitat Konstanz, Universitatsstrasse 10, 78457, Konstanz, Germany
    Arch Microbiol 181:407-17. 2004
    ..Analysis of the PCR-amplified 16S rRNA gene revealed that strain OX39 affiliates with the gram-positive endospore-forming sulfate reducers of the genus Desulfotomaculum and is the first hydrocarbon-oxidizing bacterium in this genus...
  21. ncbi Crystal structures of 2-methylisocitrate lyase in complex with product and with isocitrate inhibitor provide insight into lyase substrate specificity, catalysis and evolution
    Sijiu Liu
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Biochemistry 44:2949-62. 2005
    ..Structure-based sequence analysis and phylogenic tree construction reveal determinants of substrate specificity, highlight nodes of divergence of families, and predict enzyme families with new functions...
  22. ncbi Structure and mechanism of a sub-family of enzymes related to N-acetylneuraminate lyase
    M C Lawrence
    Biomolecular Research Institute, Parkville, Victoria, Australia
    J Mol Biol 266:381-99. 1997
    ..A fifth protein, MosA, is also confirmed to be a member of the sub-family although the involvement of an intermediate Schiff-base in its proposed reaction is unclear...
  23. pmc Cloning and characterization of a gene (UVR3) required for photorepair of 6-4 photoproducts in Arabidopsis thaliana
    S Nakajima
    Biological Institute, Graduate School of Science, Tohoku University, Sendai 980 77, Japan
    Nucleic Acids Res 26:638-44. 1998
    ....
  24. pmc Germacrene C synthase from Lycopersicon esculentum cv. VFNT cherry tomato: cDNA isolation, characterization, and bacterial expression of the multiple product sesquiterpene cyclase
    S M Colby
    U S Department of Agriculture University of California, Berkeley, Plant Gene Expression Center and Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 95:2216-21. 1998
    ....
  25. ncbi Characterization of the gene cluster for biosynthesis of macrocyclic trichothecenes in Myrothecium roridum
    S C Trapp
    Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA
    Mol Gen Genet 257:421-32. 1998
    ....
  26. ncbi Isolation and gene disruption of the Tox5 gene encoding trichodiene synthase in Gibberella pulicaris
    T M Hohn
    Mycotoxin Research Unit, National Center for Agricultural Utilization Research, Peoria, IL 61604
    Mol Plant Microbe Interact 5:249-56. 1992
    ..These results demonstrate the feasibility of using gene disruption in G. pulicaris and suggest a general method for obtaining Tox5- mutants in other trichothecene-producing fungi...
  27. pmc Quantitative exploration of the catalytic landscape separating divergent plant sesquiterpene synthases
    PAUL E O'MAILLE
    Howard Hughes Medical Institute, The Salk Institute for Biological Studies, Jack H Skirball Center for Chemical Biology and Proteomics, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Chem Biol 4:617-23. 2008
    ..These results provide a measure of the mutational accessibility of phenotypic variability in a diverging lineage of terpene synthases...
  28. ncbi The cloned 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene from Sinorhizobium sp. strain BL3 in Rhizobium sp. strain TAL1145 promotes nodulation and growth of Leucaena leucocephala
    Panlada Tittabutr
    Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East West Road, Honolulu, HI 96822, USA
    Syst Appl Microbiol 31:141-50. 2008
    ..leucocephala than by TAL1145. This study shows that the introduction of multiple copies of the acdS gene increased ACC deaminase activities of TAL1145 and enhanced its symbiotic efficiency on L. leucocephala...
  29. pmc Bacterial communities associated with flowering plants of the Ni hyperaccumulator Thlaspi goesingense
    Rughia Idris
    Department of Bioresources Microbiology, ARC Seibersdorf Research GmbH, A 2444 Seibersdorf, Austria
    Appl Environ Microbiol 70:2667-77. 2004
    ..Almost all bacteria were able to produce siderophores. Various strains, particularly endophytes, were able to grow on ACC as the sole nitrogen source...
  30. pmc Deuterium isotope effects in the unusual addition of toluene to fumarate catalyzed by benzylsuccinate synthase
    Lei Li
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biochemistry 45:13932-8. 2006
    ..2002) J. Phys. Chem. B 106, 7688-7692]. The comparison allows us to draw some conclusions on how the enzyme may catalyze this unusual reaction...
  31. ncbi Perspective of plant growth promoting rhizobacteria (PGPR) containing ACC deaminase in stress agriculture
    Muhammad Saleem
    Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan
    J Ind Microbiol Biotechnol 34:635-48. 2007
    ....
  32. ncbi Regulation of ethylene levels in canola (Brassica campestris) by 1-aminocyclopropane-1-carboxylate deaminase-containing Methylobacterium fujisawaense
    Munusamy Madhaiyan
    Department of Agricultural Chemistry, Chungbuk National University, 361 763, Cheongju, Chungbuk, Republic of Korea
    Planta 224:268-78. 2006
    ..Our results collectively suggest that Methylobacterium commonly found in soils, as well as on the surfaces of leaves, seeds, and in the rhizosphere of a wide variety of plants could be better exploited to promote plant growth...
  33. ncbi Strategies used by rhizobia to lower plant ethylene levels and increase nodulation
    Wenbo Ma
    Department of Biology, University of Waterloo, Waterloo, ON N2L 3G1, Canada
    Can J Microbiol 48:947-54. 2002
    ..This enzyme catalyzes the cleavage of ACC to alpha-ketobutyrate and ammonia. It has been shown that the inhibitory effect of ethylene on plant root elongation can be reduced by the activity of ACC deaminase...
  34. pmc Anaerobic metabolism of catechol by the denitrifying bacterium Thauera aromatica--a result of promiscuous enzymes and regulators?
    Bin Ding
    Mikrobiologie, Fakultat fur Biologie, Schänzlestr 1, D 79104 Freiburg, Germany
    J Bacteriol 190:1620-30. 2008
    ..Hence, the promiscuity of several enzymes and regulatory proteins may be sufficient to create the catechol pathway that is made up of elements of phenol, 3-hydroxybenzoate, 4-hydroxybenzoate, and benzoate metabolism...
  35. ncbi Operon structure and expression of the genes for benzylsuccinate synthase in Thauera aromatica strain K172
    Kerstin Hermuth
    Mikrobiologie, Institut fur Biologie II, Universitat Freiburg, Schanzlestrasse 1, 79104 Freiburg, Germany
    Arch Microbiol 177:132-8. 2002
    ..Furthermore, the previously known bssDCAB genes are apparently cotranscribed with a fifth gene ( bssE) whose product may function as a putative ATP-dependent chaperone for assembly and/or activation of benzylsuccinate synthase...
  36. ncbi Hydrolytic polyketide shortening by ayg1p, a novel enzyme involved in fungal melanin biosynthesis
    Isao Fujii
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biol Chem 279:44613-20. 2004
    ..Acetoacetic acid is subsequently released from the Ser257-O-acetoacetylated Ayg1p by hydrolysis. An enzyme with activity similar to Ayg1p in melanin biosynthesis has not been reported in any other organism...
  37. ncbi Evolution of enzymatic activity in the enolase superfamily: functional studies of the promiscuous o-succinylbenzoate synthase from Amycolatopsis
    Erika A Taylor Ringia
    Departments of Biochemistry and Chemistry, University of Illinois, Urbana, IL 61801, USA
    Biochemistry 43:224-9. 2004
    ....
  38. pmc Mechanism of benzylsuccinate synthase probed by substrate and isotope exchange
    Lei Li
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 128:16056-7. 2006
    ....
  39. ncbi Effects of tyrosine ring fluorination on rates and equilibria of formation of intermediates in the reactions of carbon-carbon lyases
    R S Phillips
    Department of Chemistry, University of Georgia, Athens 30602 2556, USA
    Eur J Biochem 244:658-63. 1997
    ....
  40. ncbi Mechanistic deductions from kinetic isotope effects and pH studies of pyridoxal phosphate dependent carbon-carbon lyases: Erwinia herbicola and Citrobacter freundii tyrosine phenol-lyase
    D M Kiick
    Department of Biochemistry, University of Wisconsin, Madison 53706
    Biochemistry 27:7333-8. 1988
    ..2, cvf = 24) are calculated. Also, the Michaelis complex partition ratio (cf/cvf) for substrate and products is calculated to be 4.7 for tyrosine and 0.1 for S-methyl-L-cysteine.(ABSTRACT TRUNCATED AT 250 WORDS)..
  41. ncbi Enhancement of heavy metal accumulation by tissue specific co-expression of iaaM and ACC deaminase genes in plants
    Yong Zhang
    Wuhan Institute of Virology, The Chinese Academy of Sciences, Xiaohongshan 44, Wuchang District, Wuhan 430071, China
    Chemosphere 72:564-71. 2008
    ..In addition, plants co-expressing these two genes can grow well in a complex contaminated soil containing both inorganic and organic pollutants, while the growth of the control plants was greatly inhibited...
  42. ncbi The use of tri5 gene sequences for PCR detection and taxonomy of trichothecene-producing species in the Fusarium section Sporotrichiella
    Ludwig Niessen
    Technische Universitat Munchen, Lehrstuhl für Technische Mikrobiologie, Weihenstephaner Steig 16, D 85350 Freising, Germany
    Int J Food Microbiol 95:305-19. 2004
    ..In naturally infected oats, F. langsethiae was identified by the combination of two PCR assays designed for detection of F. sporotrichioides and F. langsethiae, respectively...
  43. ncbi Rhizobacteria containing ACC-deaminase confer salt tolerance in maize grown on salt-affected fields
    Sajid Mahmood Nadeem
    Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad 38040, Pakistan
    Can J Microbiol 55:1302-9. 2009
    ..The results imply that inoculation with plant growth promoting rhizobacteria containing ACC-deaminase could be a useful approach for improving growth and yield of maize under salt-stressed conditions...
  44. ncbi Characterization of ACC deaminase gene in Pseudomonas entomophila strain PS-PJH isolated from the rhizosphere soil
    Seralathan Kamala-Kannan
    Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Korea
    J Basic Microbiol 50:200-5. 2010
    ..Based on the 16S-23S internal transcribed spacer region sequences, the isolate was identified as P. entomophila. To the best of our knowledge this is the first study to report the acdS gene in P. entomophila...
  45. pmc Phenylphosphate carboxylase: a new C-C lyase involved in anaerobic phenol metabolism in Thauera aromatica
    Karola Schühle
    Mikrobiologie, Institut Biologie II, Universitat Freiburg, Germany
    J Bacteriol 186:4556-67. 2004
    ....
  46. ncbi [Isolation and identification of hydrogen-oxidizing bacteria producing 1-aminocyclopropane-1-carboxylate deaminase and the determination of enzymatic activity]
    Bo Fu
    Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi an 710069, China
    Wei Sheng Wu Xue Bao 49:395-9. 2009
    ....
  47. ncbi Tolerance of transgenic canola plants (Brassica napus) amended with plant growth-promoting bacteria to flooding stress at a metal-contaminated field site
    Andrea J Farwell
    Department of Biology, University of Waterloo, Waterloo, Ontario, Canada
    Environ Pollut 147:540-5. 2007
    ..This is the first field study to document the increase in plant tolerance utilizing transgenic plants and plant growth-promoting bacteria exposed to multiple stressors...
  48. ncbi 1-aminocyclopropane-1-carboxylate (ACC) deaminase genes in rhizobia from southern Saskatchewan
    Jin Duan
    Department of Biology, University of Waterloo, Waterloo, ON, Canada
    Microb Ecol 57:423-36. 2009
    ..The results are discussed in the context of a previously proposed model for the regulation of bacterial ACC deaminase in R. leguminosarum 128C53K...
  49. ncbi Cloning, expression, purification and characterization of recombinant (+)-germacrene D synthase from Zingiber officinale
    Sarah Picaud
    Department of Chemistry and Biomedical Sciences, University of Kalmar, SE 39182 Kalmar, Sweden
    Arch Biochem Biophys 452:17-28. 2006
    ..The Km- and kcat-values are 0.88 microM and 3.34 x 10(-3) s(-1), respectively. A reaction mechanism involving a double 1,2-hydride shift has been established using deuterium labeled substrates in combination with GC-MS analysis...
  50. ncbi Thioesterase-like role for fungal PKS-NRPS hybrid reductive domains
    James W Sims
    Department of Medicinal Chemistry, University of Utah, 30 South 2000 East Rm 201, Salt Lake City, Utah 84112, USA
    J Am Chem Soc 130:11149-55. 2008
    ..Instead, the EqiS R catalyzes a Dieckmann condensation, with an estimated kcat approximately 15 s(-1). This role differs from the redox reactions normally catalyzed by short chain dehydrogenase/reductase superfamily enzymes...
  51. ncbi Plant growth-promoting bacteria facilitate the growth of the common reed Phragmites australisin the presence of copper or polycyclic aromatic hydrocarbons
    M L E Reed
    Department of Biology, 200 University Avenue West, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada
    Curr Microbiol 51:425-9. 2005
    ..asplenii AC to P. australis seeds enabled the plants (shoots and roots) to attain a greater size than noninoculated plants after growth in soil in the presence of either copper or creosote...
  52. pmc Infection patterns in barley and wheat spikes inoculated with wild-type and trichodiene synthase gene disrupted Fusarium graminearum
    Carin Jansen
    Institute of Phytopathology and Applied Zoology, Justus Liebig University Giessen, Germany
    Proc Natl Acad Sci U S A 102:16892-7. 2005
    ..Effective resistance to Fusarium head blight requires expression of genes that combat these different pathways of infection...
  53. ncbi New glycyl radical enzymes catalysing key metabolic steps in anaerobic bacteria
    Thorsten Selmer
    Laboratorium für Mikrobiologie, Philipps Universitat Marburg, D 35032 Marburg, Germany
    Biol Chem 386:981-8. 2005
    ....
  54. ncbi A biochemical interpretation of terpene chemotypes in Melaleuca alternifolia
    Andras Keszei
    Research School of Biology, Australian National University, Canberra 0200, Australia
    J Chem Ecol 36:652-61. 2010
    ..We found that three distinct enzymes corresponding to these groups: a sabinene-hydrate synthase, a 1,8-cineole synthase, and a terpinolene synthase may be sufficient to explain all six chemotypes in M. alternifolia...
  55. ncbi Pseudomonas brassicacearum strain Am3 containing 1-aminocyclopropane-1-carboxylate deaminase can show both pathogenic and growth-promoting properties in its interaction with tomato
    A A Belimov
    All Russia Research Institute for Agricultural Microbiology ARRIAM, Podbelskogo Sh, 3, Pushkin 8, 196608, St Petersburg, Russian Federation
    J Exp Bot 58:1485-95. 2007
    ..The results suggest that bacterial ACC deaminase of P. brassicacearum Am3 can promote growth in tomato by masking the phytopathogenic properties of this bacterium...
  56. ncbi ACC deaminase from Pseudomonas fluorescens mediated saline resistance in groundnut (Arachis hypogea) plants
    D Saravanakumar
    Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, India
    J Appl Microbiol 102:1283-92. 2007
    ..To study the effect of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase from Pseudomonas fluorescens against saline stress under in vitro and field conditions in groundnut (Arachis hypogea) plants...
  57. ncbi Cloning and expression of squalene synthase cDNA from hot pepper (Capsicum annuum L.)
    Jung Hoon Lee
    Department of Agronomy, College of Agriculture, Kyungpook National University, Daegu, Korea
    Mol Cells 13:436-43. 2002
    ..The transcript level and enzyme activity of the CASS were slightly reduced by UV. However, those of the CASC were rapidly induced within 24 h and slowly decreased thereafter...
  58. ncbi The presence of a 1-aminocyclopropane-1-carboxylate (ACC) deaminase deletion mutation alters the physiology of the endophytic plant growth-promoting bacterium Burkholderia phytofirmans PsJN
    Yili Sun
    Department of Biology, University of Waterloo, Waterloo, ON, Canada
    FEMS Microbiol Lett 296:131-6. 2009
    ..phytofirmans YS3 (B. phytofirmans YS2/pRK-AcdS) restored both ACC deaminase activity and plant growth-promotion activity in strain YS3. However, the complemented mutant still showed the above-mentioned physiological changes...
  59. ncbi The interconversion of ACC deaminase and D-cysteine desulfhydrase by directed mutagenesis
    Biljana Todorovic
    Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, ON N2L 3G1, Canada
    Planta 229:193-205. 2008
    ..Therefore, it is possible that a change in these two residues may have occurred in an ancestral protein to result in two different enzymatic activities...
  60. ncbi Escherichia coli aminodeoxychorismate synthase: analysis of pabB mutations affecting catalysis and subunit association
    E A Rayl
    Department of Biological Sciences, University of Illinois at Chicago 60607, USA
    Biochim Biophys Acta 1295:81-8. 1996
    ....
  61. ncbi Synthesis of labeled 1-amino-2-methylenecyclopropane-1-carboxylic acid, an inactivator of 1-aminocyclopropane-1-carboxylate deaminase
    Zongbao Zhao
    Division of Medicinal Chemistry, College of Pharmacy, University of Texas, Austin, TX 78712, USA
    J Org Chem 67:2509-14. 2002
    ..The synthesis of this radiolabeled compound and the results of its incubation with ACC deaminase are reported in this paper...
  62. ncbi Evolution of enzymatic activities in the enolase superfamily: crystal structures of the L-Ala-D/L-Glu epimerases from Escherichia coli and Bacillus subtilis
    A M Gulick
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 40:15716-24. 2001
    ..In this paper, we report the three-dimensional structures of these enzymes and compare them to the structure of o-succinylbenzoate synthase, another member of the muconate lactonizing enzyme subgroup...
  63. pmc Stable hydrogen and carbon isotope fractionation during microbial toluene degradation: mechanistic and environmental aspects
    B Morasch
    , , D-78457 Konstanz, Germany
    Appl Environ Microbiol 67:4842-9. 2001
    ..Identification of the first enzyme reaction in the pathway as the major fractionating step provides a basis for linking observed isotope fractionation to biochemical reactions...
  64. ncbi The in vivo synthesis of plant sesquiterpenes by Escherichia coli
    V J Martin
    Department of Chemical Engineering, University of California, Berkeley, CA 94720-1462, USA
    Biotechnol Bioeng 75:497-503. 2001
    ..coli. Based on these results, we conclude that the limiting factor for sesquiterpene synthesis in E. coli is the poor expression of the cyclase enzyme and not supply of the FDP precursor...
  65. pmc Quantification of trichothecene-producing Fusarium species in harvested grain by competitive PCR to determine efficacies of fungicides against Fusarium head blight of winter wheat
    S G Edwards
    Crop and Environment Research Centre, Harper Adams University College, Newport, Shropshire TF10 8NB, United Kingdom
    Appl Environ Microbiol 67:1575-80. 2001
    ..The Tri5 quantitative PCR assay will aid research directed towards reducing amounts of trichothecene mycotoxins in food and animal feed...
  66. ncbi Evolution of enzymatic activity in the enolase superfamily: structural and mutagenic studies of the mechanism of the reaction catalyzed by o-succinylbenzoate synthase from Escherichia coli
    Vadim A Klenchin
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53705, USA
    Biochemistry 42:14427-33. 2003
    ..The epsilon-ammonium group of Lys 235 forms a cation-pi interaction with the cyclohexadienyl moiety of SHCHC, suggesting that Lys 235 also stabilizes the enediolate anion intermediate in the syn dehydration via a similar interaction...
  67. pmc A maize sesquiterpene cyclase gene induced by insect herbivory and volicitin: characterization of wild-type and mutant alleles
    B Shen
    Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 97:14807-12. 2000
    ..Therefore, we have identified a maize gene that responds to caterpillar herbivory by producing a chemical defense signal that most likely serves to attract natural enemies of the herbivore...
  68. ncbi Role of benzylsuccinate in the induction of the tutE tutFDGH gene complex of T. aromatica strain T1
    Peter W Coschigano
    Department of Biomedical Sciences, Ohio University, Athens, OH 45701 2979, USA
    FEMS Microbiol Lett 231:261-6. 2004
    ..We conclude that benzylsuccinate is required for the full induction of the tutE tutFDGH gene cluster of T. aromatica strain T1...
  69. ncbi Syn stereochemistry of cyclic ether formation in 1,8-cineole biosynthesis catalyzed by recombinant synthase from Salvia officinalis
    Mitchell L Wise
    Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164, USA
    J Am Chem Soc 124:8546-7. 2002
    ....
  70. ncbi Genes involved in the anaerobic degradation of toluene in a denitrifying bacterium, strain EbN1
    Michael Kube
    Max Planck Institut fur Molekulare Genetik, Ihnestrasse 73, 14195 Berlin, Germany
    Arch Microbiol 181:182-94. 2004
    ..The genes between the bss and bbs operons and in the flanking regions differ between strain EbN1 and the other strains...
  71. ncbi Cloning of a sesquiterpene cyclase and its functional expression by domain swapping strategy
    K Back
    Department of Genetic Engineering, Biotechnology Research Institute, Chonnam National University, Kwangju, Korea
    Mol Cells 10:220-5. 2000
    ..The radio-labeled enzymatic products catalyzed by the partially purified chimeric CASC2 were comigrated with authentic radio-labeled sesquiterpene on thin layer chromatography...
  72. ncbi Evolution of enzymatic activity in the enolase superfamily: structure of o-succinylbenzoate synthase from Escherichia coli in complex with Mg2+ and o-succinylbenzoate
    T B Thompson
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53705, USA
    Biochemistry 39:10662-76. 2000
    ..R., Garrett, J. B., Sharma, V., Meganathan, R., Babbitt, P. C., and Gerlt, J. A. (1999) Biochemistry 38, 4252-4258]...
  73. ncbi Characterization of plant growth promoting rhizobacteria isolated from polluted soils and containing 1-aminocyclopropane-1-carboxylate deaminase
    A A Belimov
    Laboratory of Biological Nitrogen, All Russia Research Institute for Agricultural Microbiology, Saint Petersburg, Russian Federation
    Can J Microbiol 47:642-52. 2001
    ....
  74. ncbi Transcriptional regulation of the Enterobacter cloacae UW4 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene (acdS)
    J Li
    Department of Biology, University of Waterloo, ON, Canada
    Can J Microbiol 47:359-67. 2001
    ....
  75. ncbi Prevalence of 1-aminocyclopropane-1-carboxylate deaminase in Rhizobium spp
    Wenbo Ma
    Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada
    Antonie Van Leeuwenhoek 83:285-91. 2003
    ..This rhizobial ACC deaminase had a trace basal level of expression without ACC, but could be induced by a concentration of ACC as low as 1 microM. The more ACC added to this Rhizobium the higher the expression level of the ACC deaminase...
  76. ncbi Detection of anaerobic toluene and hydrocarbon degraders in contaminated aquifers using benzylsuccinate synthase (bssA) genes as a functional marker
    Christian Winderl
    Institute of Groundwater Ecology, GSF National Research Center for Environment and Health, Neuherberg, Germany
    Environ Microbiol 9:1035-46. 2007
    ....
  77. ncbi Differential response of etiolated pea seedlings to inoculation with rhizobacteria capable of utilizing 1-aminocyclopropane-1-carboxylate or L-methionine
    Baby Shaharoona
    Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad 38040, Pakistan
    J Microbiol 45:15-20. 2007
    ..This is the first study, to our knowledge, reporting on the comparative effect of rhizobacteria capable of utilizing ACC vs L-MET on etiolated pea seedlings...
  78. ncbi Plant growth-promoting and rhizosphere-competent Acinetobacter rhizosphaerae strain BIHB 723 from the cold deserts of the Himalayas
    Arvind Gulati
    Plant Pathology and Microbiology Laboratory, Hill Area Tea Sciences, Institute of Himalayan Bioresource Technology, Palampur, India
    Curr Microbiol 58:371-7. 2009
    ..The rifampicin mutant of the bacterial strain effectively colonized the pea rhizosphere without adversely affecting the resident microbial populations...
  79. ncbi Characterization of 1-aminocyclopropane-1-carboxylate (ACC) deaminase containing Methylobacterium oryzae and interactions with auxins and ACC regulation of ethylene in canola (Brassica campestris)
    Munusamy Madhaiyan
    Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk, 361 763, Republic of Korea
    Planta 226:867-76. 2007
    ..However, the inhibitory actions of exogenous additions of auxins could not be ameliorated by bacterial inoculation that reduces ethylene concentration in canola seedlings...
  80. ncbi Structural and mechanistic studies on carboxymethylproline synthase (CarB), a unique member of the crotonase superfamily catalyzing the first step in carbapenem biosynthesis
    Mark C Sleeman
    Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford OX1 3TA, United Kingdom
    J Biol Chem 280:34956-65. 2005
    ..The structural work will aid in mutagenesis studies directed at altering the selectivity of CarB to provide intermediates for the production of clinically useful carbapenems...
  81. ncbi Mechanism of benzylsuccinate synthase: stereochemistry of toluene addition to fumarate and maleate
    Chunhua Qiao
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 127:8608-9. 2005
    ....
  82. pmc Role of arginine-304 in the diphosphate-triggered active site closure mechanism of trichodiene synthase
    L Sangeetha Vedula
    Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6323, USA
    Biochemistry 44:12719-27. 2005
    ..Structural analysis of the R304K mutant and comparison with the monoterpene cyclase (+)-bornyl diphosphate synthase suggest that the significant loss in activity results from compromised activation of the PP(i) leaving group...
  83. ncbi Genes encoding the candidate enzyme for anaerobic activation of n-alkanes in the denitrifying bacterium, strain HxN1
    Olav Grundmann
    Max Planck Institut für Marine Mikrobiologie, Celsiusstrasse 1, D 28359 Bremen, Germany
    Environ Microbiol 10:376-85. 2008
    ..n-Hexane-induced co-transcription of the mas genes and additional genes of an apparent operon was demonstrated by Northern hybridization experiments...
  84. ncbi cDNA cloning of sesquiterpene cyclase and squalene synthase, and expression of the genes in potato tuber infected with Phytophthora infestans
    H Yoshioka
    Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Japan
    Plant Cell Physiol 40:993-8. 1999
    ..On the other hand, wound-induced expression of the squalene synthase was suppressed in favor of the expression of vetispiradiene synthase regardless of inoculated races...
  85. ncbi Isolation and characterization of ACC deaminase gene from two plant growth-promoting rhizobacteria
    Venkadasamy Govindasamy
    Division of Microbiology, Indian Agricultural Research Institute, New Delhi, 110012, India
    Curr Microbiol 57:312-7. 2008
    ..In the phylogenetic analysis, distinctness of these two genes was observed as a separate cluster. 16S rDNA sequencing of two isolates identified them to be Achromobacter sp. and Pseudomonas stutzeri...
  86. ncbi Changes in gene expression in canola roots induced by ACC-deaminase-containing plant-growth-promoting bacteria
    Nikos Hontzeas
    Department of Biology, University of Waterloo, Waterloo, ON N2L 3G1, Canada
    Mol Plant Microbe Interact 17:865-71. 2004
    ..This data is in agreement with a model in which ACC deaminase-containing plant-growth-promoting bacteria reduce plant stress and induce root elongation and proliferation in plants, largely by lowering ethylene levels...
  87. ncbi Effectiveness of rhizobacteria containing ACC deaminase for growth promotion of peas (Pisum sativum) under drought conditions
    Z A Zahir
    Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad 38040, Pakistan
    J Microbiol Biotechnol 18:958-63. 2008
    ..Therefore, inoculation with rhizobacteria containing ACC deaminase could be helpful in eliminating the inhibitory effects of drought stress on the growth of peas...
  88. ncbi Effect of a nickel-tolerant ACC deaminase-producing Pseudomonas strain on growth of nontransformed and transgenic canola plants
    Hilda Rodriguez
    Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada N2L 3G1
    Curr Microbiol 57:170-4. 2008
    ..The results suggest that this strain is a potential candidate to be used as an inoculant in both phytoremediation protocols and in plant growth promotion...
  89. ncbi Thiamine pyrophosphate: an essential cofactor in the mammalian metabolism of 3-methyl-branched fatty acids--implications for thiamine deficiencies?
    Veerle Foulon
    Afdeling Farmacologie, Departement Moleculaire Celbiologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, Herestraat 49, 3000 Leuven, Belgium
    Adv Exp Med Biol 544:305-6. 2003
  90. pmc Anaerobic toluene activation by benzylsuccinate synthase in a highly enriched methanogenic culture
    H R Beller
    Lawrence Livermore National Laboratory, Livermore, California 94551 0808, USA
    Appl Environ Microbiol 66:5503-5. 2000
    ..This is the first report of benzylsuccinate synthase activity in a methanogenic culture; the activity has previously been reported to occur in denitrifying, sulfate-reducing, and anoxygenic phototrophic bacteria...
  91. ncbi Substrate specificities and electron paramagnetic resonance properties of benzylsuccinate synthases in anaerobic toluene and m-xylene metabolism
    Knut Verfürth
    Mikrobiologie, Albert Ludwigs Universitat, Schanzlestrasse 1, Institut fur Biologie II, 79104 Freiburg, Germany
    Arch Microbiol 181:155-62. 2004
    ..The properties of the EPR signals are highly similar to those of the prototype glycyl radical enzyme pyruvate formate lyase, but differ slightly from previously reported parameters for partially purified benzylsuccinate synthase...
  92. ncbi Synergistic interactions between the ACC deaminase-producing bacterium Pseudomonas putida UW4 and the AM fungus Gigaspora rosea positively affect cucumber plant growth
    Elisa Gamalero
    Dipartimento di Scienze dell Ambiente e della Vita, Universita del Piemonte Orientale A Avogadro, Alessandria, Italy
    FEMS Microbiol Ecol 64:459-67. 2008
    ..Finally, the photosynthetic performance index was increased by the strain UW4 AcdS+ inoculated in combination with G. rosea BEG9. These results suggest a key role of this enzyme in the establishment and development of AM symbiosis...
  93. ncbi Transcriptional regulation of ACC deaminase gene expression in Pseudomonas putida UW4
    Zhenyu Cheng
    Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, ON N2L3G1, Canada
    Can J Microbiol 54:128-36. 2008
    ..The data are interpreted in terms of a model in which AcdR and AcdB co-regulate the expression of the acdS gene...
  94. pmc Residues C123 and D58 of the 2-methylisocitrate lyase (PrpB) enzyme of Salmonella enterica are essential for catalysis
    T L Grimek
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53726 4087, USA
    J Bacteriol 185:4837-43. 2003
    ..Based on the proposed mechanism of the closely related isocitrate lyases, PrpB residue C123 is proposed to serve as the active site base, and residue D58 is critical for the coordination of a required Mg(2+) ion...
  95. ncbi Role of menaquinone biosynthesis genes in selenate reduction by Enterobacter cloacae SLD1a-1 and Escherichia coli K12
    Jincai Ma
    Department of Environmental Sciences, Rutgers, State University of New Jersey, New Brunswick, NJ, USA
    Environ Microbiol 11:149-58. 2009
    ..The results of this work suggest that menaquinones are an important source of electrons for the selenate reductase, and are required for selenate reduction activity in E. cloacae SLD1a-1 and E. coli K12...
  96. ncbi Substrate range of benzylsuccinate synthase from Azoarcus sp. strain T
    H R Beller
    Department of Civil and Environmental Engineering, Stanford University, CA 94305 4020, USA
    FEMS Microbiol Lett 178:147-53. 1999
    ..The implications of these observations for the mechanism of benzylsuccinate synthase will be discussed...
  97. ncbi Plant growth-promoting trait of rhizobacteria isolated from soil contaminated with petroleum and heavy metals
    So Yeon Koo
    Department of Environmental Science and Engineering, Ewha Womans University, Seoul 120 750, Korea
    J Microbiol Biotechnol 20:587-93. 2010
    ..Clustering analysis by principal component analysis showed that RP was the most important factor influencing ecological distribution and physiological characterization of PGPP-possesing rhizobateria...
  98. pmc Structure of epi-isozizaene synthase from Streptomyces coelicolor A3(2), a platform for new terpenoid cyclization templates
    Julie A Aaron
    Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6323, USA
    Biochemistry 49:1787-97. 2010
    ..Finally, the crystal structure of wild-type EIZS in a complex with four Hg(2+) ions has been determined at 1.90 A resolution, showing that metal binding triggers a significant conformational change of helix G to cap the active site...
  99. pmc Kinetic characterization of 4-amino 4-deoxychorismate synthase from Escherichia coli
    V K Viswanathan
    Department of Biological Sciences, University of Illinois at Chicago 60607 7060, USA
    J Bacteriol 177:5918-23. 1995
    ..No inhibition of ADC synthase activity was observed when p-aminobenzoate, sulfanilamide, sulfathiazole, and several compounds requiring folate for their biosynthesis were used...
  100. ncbi Anaerobic metabolism of phenol in proteobacteria and further studies of phenylphosphate carboxylase
    Sirko Schmeling
    Mikrobiologie, Fakultat fur Biologie, Universitat Freiburg, Schänzlestr 1, 79104, Freiburg, Germany
    Arch Microbiol 191:869-78. 2009
    ....
  101. pmc ACC (1-aminocyclopropane-1-carboxylate) deaminase activity, a widespread trait in Burkholderia species, and its growth-promoting effect on tomato plants
    Janette Onofre-Lemus
    Centro de Ciencias Genomicas, Universidad Nacional Autonoma de Mexico, Ap Postal 565 A, Cuernavaca, Morelos, Mexico
    Appl Environ Microbiol 75:6581-90. 2009
    ..The widespread ACC deaminase activity in Burkholderia species and the common association of these species with plants suggest that this genus could be a major contributor to plant growth under natural conditions...

Research Grants3

  1. ENZYME CATALYSIS OF ELECTRON AND GROUP TRANSFER
    PERRY FREY; Fiscal Year: 2006
    ..The contributions of beta- amino acids produced by lysine 2,3-aminomutase and arginine 2,3-aminomutase to the functions of antibiotics are not known. The beta-aminoacids may become significant in drug development. ..
  2. MECHANISMS OF ENZYMATIC REACTIONS
    PERRY FREY; Fiscal Year: 2006
    ..unreadable] [unreadable]..