carbon carbon lyases

Summary

Summary: Enzymes that catalyze the cleavage of a carbon-carbon bond by means other than hydrolysis or oxidation. This subclass contains the DECARBOXYLASES, the ALDEHYDE-LYASES, and the OXO-ACID-LYASES. EC 4.1.

Top Publications

  1. doi Physical organization and phylogenetic analysis of acdR as leucine-responsive regulator of the 1-aminocyclopropane-1-carboxylate deaminase gene acdS in phytobeneficial Azospirillum lipoferum 4B and other Proteobacteria
    Claire Prigent-Combaret
    Universite de Lyon, Lyon, F 69003, France
    FEMS Microbiol Ecol 65:202-19. 2008
  2. ncbi A single-vial analytical and quantitative gas chromatography-mass spectrometry assay for terpene synthases
    PAUL E O'MAILLE
    Structural Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Anal Biochem 335:210-7. 2004
  3. pmc Evolving biosynthetic tangos negotiate mechanistic landscapes
    Michael B Austin
    The Salk Institute for Biological Studies, Jack H Skirball Center for Chemical Biology and Proteomics, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Chem Biol 4:217-22. 2008
  4. ncbi Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum
    Barbara Morasch
    Fakultat fur Biologie, Universitat Konstanz, Universitatsstrasse 10, 78457, Konstanz, Germany
    Arch Microbiol 181:407-17. 2004
  5. ncbi Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase
    Matthias Brock
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
    Eur J Biochem 269:6184-94. 2002
  6. ncbi Response of spring rape (Brassica napus var. oleifera L.) to inoculation with plant growth promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase depends on nutrient status of the plant
    Andrei A Belimov
    Laboratory of Ecology of Symbiotic and Associative Bacteria, All Russia Research Institute for Agricultural Microbiology, Saint Petersburg
    Can J Microbiol 48:189-99. 2002
  7. pmc Quantitative exploration of the catalytic landscape separating divergent plant sesquiterpene synthases
    PAUL E O'MAILLE
    Howard Hughes Medical Institute, The Salk Institute for Biological Studies, Jack H Skirball Center for Chemical Biology and Proteomics, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Chem Biol 4:617-23. 2008
  8. ncbi Expression and characterization of 1-aminocyclopropane-1-carboxylate deaminase from the rhizobacterium Pseudomonas putida UW4: a key enzyme in bacterial plant growth promotion
    Nikos Hontzeas
    Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada N2L 3G1
    Biochim Biophys Acta 1703:11-9. 2004
  9. pmc Aerobic and anaerobic toluene degradation by a newly isolated denitrifying bacterium, Thauera sp. strain DNT-1
    Yoshifumi Shinoda
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Appl Environ Microbiol 70:1385-92. 2004
  10. doi The cloned 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene from Sinorhizobium sp. strain BL3 in Rhizobium sp. strain TAL1145 promotes nodulation and growth of Leucaena leucocephala
    Panlada Tittabutr
    Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East West Road, Honolulu, HI 96822, USA
    Syst Appl Microbiol 31:141-50. 2008

Research Grants

  1. MECHANISMS OF CARBON-CARBON LYASES AND KYNURENINASE
    Robert Phillips; Fiscal Year: 1993
  2. BIOSYNTHESIS OF MICROBIAL PRODUCTS
    DAVID CANE; Fiscal Year: 2000
  3. Biosynthesis of Microbial Isoprenoids
    David E Cane; Fiscal Year: 2010
  4. Biosynthesis of Microbial Polyketides
    DAVID CANE; Fiscal Year: 2009
  5. Biosynthesis of Microbial Polyketides
    DAVID CANE; Fiscal Year: 2007
  6. STEREOCHEMICAL STUDIES OF ISOPRENOID BIOSYNTHESIS
    DAVID CANE; Fiscal Year: 2007
  7. Biosynthesis of Microbial Polyketides
    DAVID CANE; Fiscal Year: 2005
  8. Biosynthesis of Microbial Polyketides
    David E Cane; Fiscal Year: 2010
  9. BIOSYNTHESIS OF ANTIBIOTIC NATURAL PRODUCTS
    DAVID CANE; Fiscal Year: 1980
  10. BIOSYNTHESIS OF MICROBIAL NATURAL PRODUCTS
    DAVID CANE; Fiscal Year: 1993

Scientific Experts

Detail Information

Publications109 found, 100 shown here

  1. doi Physical organization and phylogenetic analysis of acdR as leucine-responsive regulator of the 1-aminocyclopropane-1-carboxylate deaminase gene acdS in phytobeneficial Azospirillum lipoferum 4B and other Proteobacteria
    Claire Prigent-Combaret
    Universite de Lyon, Lyon, F 69003, France
    FEMS Microbiol Ecol 65:202-19. 2008
    ..lipoferum 4B. This is the first synteny, phylogenetic, and functional analysis of factors modulating acdS expression in Azospirillum plant growth-promoting rhizobacterium...
  2. ncbi A single-vial analytical and quantitative gas chromatography-mass spectrometry assay for terpene synthases
    PAUL E O'MAILLE
    Structural Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Anal Biochem 335:210-7. 2004
    ....
  3. pmc Evolving biosynthetic tangos negotiate mechanistic landscapes
    Michael B Austin
    The Salk Institute for Biological Studies, Jack H Skirball Center for Chemical Biology and Proteomics, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Chem Biol 4:217-22. 2008
    ..Evolution of these enzyme families relies on rhythmic tangos, in which the enzymes and substrates together determine product outcome by negotiating decision networks governing intrinsic and induced chemical reactivities...
  4. ncbi Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum
    Barbara Morasch
    Fakultat fur Biologie, Universitat Konstanz, Universitatsstrasse 10, 78457, Konstanz, Germany
    Arch Microbiol 181:407-17. 2004
    ..Analysis of the PCR-amplified 16S rRNA gene revealed that strain OX39 affiliates with the gram-positive endospore-forming sulfate reducers of the genus Desulfotomaculum and is the first hydrocarbon-oxidizing bacterium in this genus...
  5. ncbi Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase
    Matthias Brock
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
    Eur J Biochem 269:6184-94. 2002
    ..This is in good agreement with investigations on Salmonella enterica LT2, in which disruption of the prpE gene showed no visible phenotype...
  6. ncbi Response of spring rape (Brassica napus var. oleifera L.) to inoculation with plant growth promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase depends on nutrient status of the plant
    Andrei A Belimov
    Laboratory of Ecology of Symbiotic and Associative Bacteria, All Russia Research Institute for Agricultural Microbiology, Saint Petersburg
    Can J Microbiol 48:189-99. 2002
    ..The results obtained show that the growth-promoting effects of ACC-utilizing rhizobacteria depend significantly on the nutrient status of the plant...
  7. pmc Quantitative exploration of the catalytic landscape separating divergent plant sesquiterpene synthases
    PAUL E O'MAILLE
    Howard Hughes Medical Institute, The Salk Institute for Biological Studies, Jack H Skirball Center for Chemical Biology and Proteomics, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Chem Biol 4:617-23. 2008
    ..These results provide a measure of the mutational accessibility of phenotypic variability in a diverging lineage of terpene synthases...
  8. ncbi Expression and characterization of 1-aminocyclopropane-1-carboxylate deaminase from the rhizobacterium Pseudomonas putida UW4: a key enzyme in bacterial plant growth promotion
    Nikos Hontzeas
    Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada N2L 3G1
    Biochim Biophys Acta 1703:11-9. 2004
    ..This enzyme's biochemical and biophysical properties will serve as an important reference point to which newly isolated ACC deaminases from other organisms can be compared...
  9. pmc Aerobic and anaerobic toluene degradation by a newly isolated denitrifying bacterium, Thauera sp. strain DNT-1
    Yoshifumi Shinoda
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Appl Environ Microbiol 70:1385-92. 2004
    ..On the basis of these results, it is concluded that strain DNT-1 modulates the expression of two different initial pathways of toluene degradation according to the availability of oxygen in the environment...
  10. doi The cloned 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene from Sinorhizobium sp. strain BL3 in Rhizobium sp. strain TAL1145 promotes nodulation and growth of Leucaena leucocephala
    Panlada Tittabutr
    Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East West Road, Honolulu, HI 96822, USA
    Syst Appl Microbiol 31:141-50. 2008
    ..leucocephala than by TAL1145. This study shows that the introduction of multiple copies of the acdS gene increased ACC deaminase activities of TAL1145 and enhanced its symbiotic efficiency on L. leucocephala...
  11. ncbi Reaction of 1-amino-2-methylenecyclopropane-1-carboxylate with 1-aminocyclopropane-1-carboxylate deaminase: analysis and mechanistic implications
    Zongbao Zhao
    Division of Medicinal Chemistry, College of Pharmacy, University of Texas, Austin, Texas 78712, USA
    Biochemistry 42:2089-103. 2003
    ..Clearly, the cleavage of ACC to alpha-ketobutyrate by ACC deaminase represents an intriguing conversion beyond the common scope entailed by coenzyme B(6) dependent catalysts...
  12. ncbi Phytanic acid alpha-oxidation, new insights into an old problem: a review
    Ronald J A Wanders
    Laboratory Genetic Metabolic Diseases, Department of Pediatrics Emma Children s Hospital and Clinical Chemistry, Academic Medical Centre, University Hospital Amsterdam, Room F0 224, P O Box 22700, 1100 DE Amsterdam, The Netherlands
    Biochim Biophys Acta 1631:119-35. 2003
    ..This Review describes the current state of knowledge about fatty acid alpha-oxidation in mammals with particular emphasis on the mechanism involved and the enzymology of the pathway...
  13. ncbi Crystal structure of 2-methylisocitrate lyase (PrpB) from Escherichia coli and modelling of its ligand bound active centre
    Clemens Grimm
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    J Mol Biol 328:609-21. 2003
    ....
  14. ncbi Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-beneficial properties
    A Sessitsch
    ARC Seibersdorf Research GmbH, Department of Bioresources Microbiology, Austria
    Int J Syst Evol Microbiol 55:1187-92. 2005
    ..nov. is proposed. The type strain is strain PsJN(T) (=LMG 22146(T) = CCUG 49060(T))...
  15. ncbi Carboxymethylproline synthase (CarB), an unusual carbon-carbon bond-forming enzyme of the crotonase superfamily involved in carbapenem biosynthesis
    Mark C Sleeman
    Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, UK
    J Biol Chem 279:6730-6. 2004
    ..The implications of the results for the biosynthesis of both carbapenem-3-carboxylate and C-2/C-6-substituted carbapenems, such as thienamycin, are discussed...
  16. ncbi Crystal structures of 2-methylisocitrate lyase in complex with product and with isocitrate inhibitor provide insight into lyase substrate specificity, catalysis and evolution
    Sijiu Liu
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Biochemistry 44:2949-62. 2005
    ..Structure-based sequence analysis and phylogenic tree construction reveal determinants of substrate specificity, highlight nodes of divergence of families, and predict enzyme families with new functions...
  17. ncbi Genes involved in the anaerobic degradation of toluene in a denitrifying bacterium, strain EbN1
    Michael Kube
    Max Planck Institut fur Molekulare Genetik, Ihnestrasse 73, 14195 Berlin, Germany
    Arch Microbiol 181:182-94. 2004
    ..The genes between the bss and bbs operons and in the flanking regions differ between strain EbN1 and the other strains...
  18. ncbi Evolution of enzymatic activities in the enolase superfamily: crystal structures of the L-Ala-D/L-Glu epimerases from Escherichia coli and Bacillus subtilis
    A M Gulick
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 40:15716-24. 2001
    ..In this paper, we report the three-dimensional structures of these enzymes and compare them to the structure of o-succinylbenzoate synthase, another member of the muconate lactonizing enzyme subgroup...
  19. ncbi Isolation and gene disruption of the Tox5 gene encoding trichodiene synthase in Gibberella pulicaris
    T M Hohn
    Mycotoxin Research Unit, National Center for Agricultural Utilization Research, Peoria, IL 61604
    Mol Plant Microbe Interact 5:249-56. 1992
    ..These results demonstrate the feasibility of using gene disruption in G. pulicaris and suggest a general method for obtaining Tox5- mutants in other trichothecene-producing fungi...
  20. pmc Germacrene C synthase from Lycopersicon esculentum cv. VFNT cherry tomato: cDNA isolation, characterization, and bacterial expression of the multiple product sesquiterpene cyclase
    S M Colby
    U S Department of Agriculture University of California, Berkeley, Plant Gene Expression Center and Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 95:2216-21. 1998
    ....
  21. ncbi Characterization of the gene cluster for biosynthesis of macrocyclic trichothecenes in Myrothecium roridum
    S C Trapp
    Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA
    Mol Gen Genet 257:421-32. 1998
    ....
  22. ncbi Cloning, expression, purification and characterization of recombinant (+)-germacrene D synthase from Zingiber officinale
    Sarah Picaud
    Department of Chemistry and Biomedical Sciences, University of Kalmar, SE 39182 Kalmar, Sweden
    Arch Biochem Biophys 452:17-28. 2006
    ..The Km- and kcat-values are 0.88 microM and 3.34 x 10(-3) s(-1), respectively. A reaction mechanism involving a double 1,2-hydride shift has been established using deuterium labeled substrates in combination with GC-MS analysis...
  23. ncbi MosA, a protein implicated in rhizopine biosynthesis in Sinorhizobium meliloti L5-30, is a dihydrodipicolinate synthase
    Pui Hang Tam
    Department of Biochemistry, University of Saskatchewan, 110 Science Place, Saskatoon, Sask, Canada S7N 5C9
    J Mol Biol 335:393-7. 2004
    ..A role for this enzyme in rhizopine synthesis cannot be ruled out, but is called into question...
  24. ncbi Structural and enzymatic properties of 1-aminocyclopropane-1-carboxylate deaminase homologue from Pyrococcus horikoshii
    Aiko Fujino
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan
    J Mol Biol 341:999-1013. 2004
    ..We also propose a condition necessary in the primary sequence to have ACCD activity...
  25. ncbi Structure and mechanism of a sub-family of enzymes related to N-acetylneuraminate lyase
    M C Lawrence
    Biomolecular Research Institute, Parkville, Victoria, Australia
    J Mol Biol 266:381-99. 1997
    ..A fifth protein, MosA, is also confirmed to be a member of the sub-family although the involvement of an intermediate Schiff-base in its proposed reaction is unclear...
  26. ncbi Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries
    Joost Lücker
    Biotechnology Laboratory, Department of Botany, University of British Columbia, 6174 University Boulevard, Vancouver, BC, V6T 1Z3, Canada
    Phytochemistry 65:2649-59. 2004
    ..Sesquiterpene synthase transcripts were also detected in young seeds...
  27. ncbi Evolution of structure and function in the o-succinylbenzoate synthase/N-acylamino acid racemase family of the enolase superfamily
    Margaret E Glasner
    Department of Biopharmaceutical Sciences, University of California, San Francisco, CA 94143, USA
    J Mol Biol 360:228-50. 2006
    ..Finally, a combination of evolutionary, structural, and sequence analyses identified characteristics that might prime proteins, such as Amycolatopsis OSBS/NAAAR, for the evolution of new activities...
  28. ncbi Evolution of enzymatic activity in the enolase superfamily: structure of o-succinylbenzoate synthase from Escherichia coli in complex with Mg2+ and o-succinylbenzoate
    T B Thompson
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53705, USA
    Biochemistry 39:10662-76. 2000
    ..R., Garrett, J. B., Sharma, V., Meganathan, R., Babbitt, P. C., and Gerlt, J. A. (1999) Biochemistry 38, 4252-4258]...
  29. ncbi Biochemical and genetic evidence of benzylsuccinate synthase in toluene-degrading, ferric iron-reducing Geobacter metallireducens
    Staci R Kane
    Lawrence Livermore National Laboratory, Livermore, CA 94551 0808, USA
    Biodegradation 13:149-54. 2002
    ....
  30. doi The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds
    Vitaly Portnoy
    Institute of Plant Sciences, Newe Ya ar Research Center, Agricultural Research Organization, P O Box 1021, Ramat Yishay 30095, Israel
    Plant Mol Biol 66:647-61. 2008
    ..The genes are differentially transcriptionally regulated during fruit development and according to variety and are likely to be associated with chemical differences responsible for the unique aromas of melon varieties...
  31. pmc Depth-resolved quantification of anaerobic toluene degraders and aquifer microbial community patterns in distinct redox zones of a tar oil contaminant plume
    Christian Winderl
    Institute of Groundwater Ecology, Helmholtz Center Munich, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764 Neuherberg, Germany
    Appl Environ Microbiol 74:792-801. 2008
    ....
  32. pmc Cloning and characterization of a gene (UVR3) required for photorepair of 6-4 photoproducts in Arabidopsis thaliana
    S Nakajima
    Biological Institute, Graduate School of Science, Tohoku University, Sendai 980 77, Japan
    Nucleic Acids Res 26:638-44. 1998
    ....
  33. pmc Bacterial communities associated with flowering plants of the Ni hyperaccumulator Thlaspi goesingense
    Rughia Idris
    Department of Bioresources Microbiology, ARC Seibersdorf Research GmbH, A 2444 Seibersdorf, Austria
    Appl Environ Microbiol 70:2667-77. 2004
    ..Almost all bacteria were able to produce siderophores. Various strains, particularly endophytes, were able to grow on ACC as the sole nitrogen source...
  34. ncbi Differential response of etiolated pea seedlings to inoculation with rhizobacteria capable of utilizing 1-aminocyclopropane-1-carboxylate or L-methionine
    Baby Shaharoona
    Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad 38040, Pakistan
    J Microbiol 45:15-20. 2007
    ..This is the first study, to our knowledge, reporting on the comparative effect of rhizobacteria capable of utilizing ACC vs L-MET on etiolated pea seedlings...
  35. pmc Deuterium isotope effects in the unusual addition of toluene to fumarate catalyzed by benzylsuccinate synthase
    Lei Li
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biochemistry 45:13932-8. 2006
    ..2002) J. Phys. Chem. B 106, 7688-7692]. The comparison allows us to draw some conclusions on how the enzyme may catalyze this unusual reaction...
  36. ncbi Regulation of ethylene levels in canola (Brassica campestris) by 1-aminocyclopropane-1-carboxylate deaminase-containing Methylobacterium fujisawaense
    Munusamy Madhaiyan
    Department of Agricultural Chemistry, Chungbuk National University, 361 763, Cheongju, Chungbuk, Republic of Korea
    Planta 224:268-78. 2006
    ..Our results collectively suggest that Methylobacterium commonly found in soils, as well as on the surfaces of leaves, seeds, and in the rhizosphere of a wide variety of plants could be better exploited to promote plant growth...
  37. ncbi Perspective of plant growth promoting rhizobacteria (PGPR) containing ACC deaminase in stress agriculture
    Muhammad Saleem
    Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan
    J Ind Microbiol Biotechnol 34:635-48. 2007
    ....
  38. ncbi Characterization of 1-aminocyclopropane-1-carboxylate (ACC) deaminase containing Methylobacterium oryzae and interactions with auxins and ACC regulation of ethylene in canola (Brassica campestris)
    Munusamy Madhaiyan
    Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk, 361 763, Republic of Korea
    Planta 226:867-76. 2007
    ..However, the inhibitory actions of exogenous additions of auxins could not be ameliorated by bacterial inoculation that reduces ethylene concentration in canola seedlings...
  39. ncbi Strategies used by rhizobia to lower plant ethylene levels and increase nodulation
    Wenbo Ma
    Department of Biology, University of Waterloo, Waterloo, ON N2L 3G1, Canada
    Can J Microbiol 48:947-54. 2002
    ..This enzyme catalyzes the cleavage of ACC to alpha-ketobutyrate and ammonia. It has been shown that the inhibitory effect of ethylene on plant root elongation can be reduced by the activity of ACC deaminase...
  40. pmc Anaerobic metabolism of catechol by the denitrifying bacterium Thauera aromatica--a result of promiscuous enzymes and regulators?
    Bin Ding
    Mikrobiologie, Fakultat fur Biologie, Schänzlestr 1, D 79104 Freiburg, Germany
    J Bacteriol 190:1620-30. 2008
    ..Hence, the promiscuity of several enzymes and regulatory proteins may be sufficient to create the catechol pathway that is made up of elements of phenol, 3-hydroxybenzoate, 4-hydroxybenzoate, and benzoate metabolism...
  41. pmc Phenylphosphate carboxylase: a new C-C lyase involved in anaerobic phenol metabolism in Thauera aromatica
    Karola Schühle
    Mikrobiologie, Institut Biologie II, Universitat Freiburg, Germany
    J Bacteriol 186:4556-67. 2004
    ....
  42. ncbi Role of benzylsuccinate in the induction of the tutE tutFDGH gene complex of T. aromatica strain T1
    Peter W Coschigano
    Department of Biomedical Sciences, Ohio University, Athens, OH 45701 2979, USA
    FEMS Microbiol Lett 231:261-6. 2004
    ..We conclude that benzylsuccinate is required for the full induction of the tutE tutFDGH gene cluster of T. aromatica strain T1...
  43. ncbi Operon structure and expression of the genes for benzylsuccinate synthase in Thauera aromatica strain K172
    Kerstin Hermuth
    Mikrobiologie, Institut fur Biologie II, Universitat Freiburg, Schanzlestrasse 1, 79104 Freiburg, Germany
    Arch Microbiol 177:132-8. 2002
    ..Furthermore, the previously known bssDCAB genes are apparently cotranscribed with a fifth gene ( bssE) whose product may function as a putative ATP-dependent chaperone for assembly and/or activation of benzylsuccinate synthase...
  44. ncbi Hydrolytic polyketide shortening by ayg1p, a novel enzyme involved in fungal melanin biosynthesis
    Isao Fujii
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biol Chem 279:44613-20. 2004
    ..Acetoacetic acid is subsequently released from the Ser257-O-acetoacetylated Ayg1p by hydrolysis. An enzyme with activity similar to Ayg1p in melanin biosynthesis has not been reported in any other organism...
  45. ncbi Structural and mechanistic studies on carboxymethylproline synthase (CarB), a unique member of the crotonase superfamily catalyzing the first step in carbapenem biosynthesis
    Mark C Sleeman
    Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford OX1 3TA, United Kingdom
    J Biol Chem 280:34956-65. 2005
    ..The structural work will aid in mutagenesis studies directed at altering the selectivity of CarB to provide intermediates for the production of clinically useful carbapenems...
  46. ncbi Evolution of enzymatic activity in the enolase superfamily: functional studies of the promiscuous o-succinylbenzoate synthase from Amycolatopsis
    Erika A Taylor Ringia
    Departments of Biochemistry and Chemistry, University of Illinois, Urbana, IL 61801, USA
    Biochemistry 43:224-9. 2004
    ....
  47. pmc Mechanism of benzylsuccinate synthase probed by substrate and isotope exchange
    Lei Li
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 128:16056-7. 2006
    ....
  48. ncbi Effects of tyrosine ring fluorination on rates and equilibria of formation of intermediates in the reactions of carbon-carbon lyases
    R S Phillips
    Department of Chemistry, University of Georgia, Athens 30602 2556, USA
    Eur J Biochem 244:658-63. 1997
    ....
  49. ncbi Mechanistic deductions from kinetic isotope effects and pH studies of pyridoxal phosphate dependent carbon-carbon lyases: Erwinia herbicola and Citrobacter freundii tyrosine phenol-lyase
    D M Kiick
    Department of Biochemistry, University of Wisconsin, Madison 53706
    Biochemistry 27:7333-8. 1988
    ..2, cvf = 24) are calculated. Also, the Michaelis complex partition ratio (cf/cvf) for substrate and products is calculated to be 4.7 for tyrosine and 0.1 for S-methyl-L-cysteine.(ABSTRACT TRUNCATED AT 250 WORDS)..
  50. ncbi Evidence for p-hydroxybenzoate formation involving enzymatic phenylpropanoid side-chain cleavage in hairy roots of Daucus carota
    Debabrata Sircar
    Agricultural and Food Engineering Department, Natural Product Biotechnology Group, Indian Institute of Technology Kharagpur, Kharagpur, India
    J Plant Physiol 165:407-14. 2008
    ..4) and the temperature optimum was found to be at 34 degrees C. The MJ-treated roots showed highest HBS activity at 24h (52nkat/mg protein), which was nearly 5-fold higher than that in the control lines...
  51. ncbi Genes encoding the candidate enzyme for anaerobic activation of n-alkanes in the denitrifying bacterium, strain HxN1
    Olav Grundmann
    Max Planck Institut für Marine Mikrobiologie, Celsiusstrasse 1, D 28359 Bremen, Germany
    Environ Microbiol 10:376-85. 2008
    ..n-Hexane-induced co-transcription of the mas genes and additional genes of an apparent operon was demonstrated by Northern hybridization experiments...
  52. ncbi Dominance of Geobacteraceae in BTX-degrading enrichments from an iron-reducing aquifer
    Sabrina Botton
    Earth Surface Processes and Materials Department, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands
    FEMS Microbiol Ecol 62:118-30. 2007
    ..Combined with previous studies, this research suggests that Geobacteraceae play a key role in the natural attenuation of each BTX compound in situ...
  53. ncbi Regulation of simultaneous synthesis of floral scent terpenoids by the 1,8-cineole synthase of Nicotiana suaveolens
    Susanna Roeder
    Institute of Biological Sciences, University of Rostock, Albert Einstein Str 3, Rostock, Germany
    Plant Mol Biol 65:107-24. 2007
    ..suaveolens flowers: (i) the synthesis of volatile organic compounds by a multi-product enzyme and (ii) the coordination of biosynthetic pathways by a circadian clock...
  54. pmc Exploring biosynthetic diversity with trichodiene synthase
    L Sangeetha Vedula
    Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104 6323, USA
    Arch Biochem Biophys 466:260-6. 2007
    ....
  55. pmc Sebacina vermifera promotes the growth and fitness of Nicotiana attenuata by inhibiting ethylene signaling
    Oz Barazani
    Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Beutenberg Campus, 07745 Jena, Germany
    Plant Physiol 144:1223-32. 2007
    ..In contrast, neither inoculation nor KMnO(4) exposure influenced the germination of ir-aco seeds. We conclude that S. vermifera increases plant growth by impairing ethylene production independently of JA signaling and TPI production...
  56. pmc Properties of R-citramalyl-coenzyme A lyase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus
    Silke Friedmann
    Mikrobiologie, Institut Biologie II, Schänzlestr 1, D 79104 Freiburg, Germany
    J Bacteriol 189:2906-14. 2007
    ..Incomplete sets of genes were found in aerobic phototrophic bacteria and in the gamma-proteobacterium Congregibacter litoralis. This may indicate that part of the reactions may be involved in a different metabolic process...
  57. doi myo-Inositol catabolism in Bacillus subtilis
    Ken ichi Yoshida
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University Kobe 657 8501, Japan
    J Biol Chem 283:10415-24. 2008
    ..The net result of the inositol catabolic pathway in B. subtilis is, thus, the conversion of myo-inositol to an equimolar mixture of dihydroxyacetone phosphate, acetyl-CoA, and CO(2)...
  58. pmc Structure of epi-isozizaene synthase from Streptomyces coelicolor A3(2), a platform for new terpenoid cyclization templates
    Julie A Aaron
    Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6323, USA
    Biochemistry 49:1787-97. 2010
    ..Finally, the crystal structure of wild-type EIZS in a complex with four Hg(2+) ions has been determined at 1.90 A resolution, showing that metal binding triggers a significant conformational change of helix G to cap the active site...
  59. pmc ACC (1-aminocyclopropane-1-carboxylate) deaminase activity, a widespread trait in Burkholderia species, and its growth-promoting effect on tomato plants
    Janette Onofre-Lemus
    Centro de Ciencias Genomicas, Universidad Nacional Autonoma de Mexico, Ap Postal 565 A, Cuernavaca, Morelos, Mexico
    Appl Environ Microbiol 75:6581-90. 2009
    ..The widespread ACC deaminase activity in Burkholderia species and the common association of these species with plants suggest that this genus could be a major contributor to plant growth under natural conditions...
  60. doi Ethylene levels are regulated by a plant encoded 1-aminocyclopropane-1-carboxylic acid deaminase
    Lisa McDonnell
    Department of Biology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Physiol Plant 136:94-109. 2009
    ..Taken together, these results show that AtACD1 can act as a regulator of ACC levels in A. thaliana...
  61. pmc A versatile photoactivatable probe designed to label the diphosphate binding site of farnesyl diphosphate utilizing enzymes
    Olivier Henry
    Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, United States
    Bioorg Med Chem 17:4797-805. 2009
    ..Taken together, these results using a number of enzymes demonstrate that this new class of probes should be useful for a plethora of studies of FPP-utilizing enzymes...
  62. doi A multi-gene phylogeny for Stachybotrys evidences lack of trichodiene synthase (tri5) gene for isolates of one of three intrageneric lineages
    Brenda Koster
    Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada
    Mycol Res 113:877-86. 2009
    ..Moreover, sites rates analysis showed that tri5 was highly conserved at the amino acid level suggesting that identity at variable sites, among otherwise divergent taxa, might be the result of chance events...
  63. doi Sesquiterpene variability in the gorgonian genus Plexaurella
    Jamie L Frenz-Ross
    Department of Chemistry and Biochemistry and Center of Excellence in Biomedical and Marine Biotechnology, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431, USA
    Comp Biochem Physiol C Toxicol Pharmacol 150:125-31. 2009
    ..There was also no correlation between location or depth and sesquiterpene content. We therefore conclude that Plexaurella spp. are chemically indistinguishable species with respect to terpene chemistry...
  64. doi Rhizosphere bacteria containing 1-aminocyclopropane-1-carboxylate deaminase increase yield of plants grown in drying soil via both local and systemic hormone signalling
    Andrey A Belimov
    All Russia Research Institute for Agricultural Microbiology, Podbelskogo Sh 3, Pushkin 8, 196608, St Petersburg, Russian Federation
    New Phytol 181:413-23. 2009
    ..Such bacteria may provide an easily realized, economic means of sustaining crop yields and using irrigation water more efficiently in dryland agriculture...
  65. doi Long-term effect of a single application of organic refuse on carbon sequestration and soil physical properties
    J Albaladejo
    Dpto de Conservación de Suelos y Aguas, Centro de Edaf ología y Biología Aplicada del Segura, CEBAS CSIC, P O Box 164, 30100, Murcia, Spain
    J Environ Qual 37:2093-9. 2008
    ..Clearly, a single addition of organic refuse to the degraded soils to increase the potential for C sequestration was effective...
  66. doi Role of menaquinone biosynthesis genes in selenate reduction by Enterobacter cloacae SLD1a-1 and Escherichia coli K12
    Jincai Ma
    Department of Environmental Sciences, Rutgers, State University of New Jersey, New Brunswick, NJ, USA
    Environ Microbiol 11:149-58. 2009
    ..The results of this work suggest that menaquinones are an important source of electrons for the selenate reductase, and are required for selenate reduction activity in E. cloacae SLD1a-1 and E. coli K12...
  67. doi Isolation and characterization of ACC deaminase gene from two plant growth-promoting rhizobacteria
    Venkadasamy Govindasamy
    Division of Microbiology, Indian Agricultural Research Institute, New Delhi, 110012, India
    Curr Microbiol 57:312-7. 2008
    ..In the phylogenetic analysis, distinctness of these two genes was observed as a separate cluster. 16S rDNA sequencing of two isolates identified them to be Achromobacter sp. and Pseudomonas stutzeri...
  68. ncbi Effectiveness of rhizobacteria containing ACC deaminase for growth promotion of peas (Pisum sativum) under drought conditions
    Z A Zahir
    Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad 38040, Pakistan
    J Microbiol Biotechnol 18:958-63. 2008
    ..Therefore, inoculation with rhizobacteria containing ACC deaminase could be helpful in eliminating the inhibitory effects of drought stress on the growth of peas...
  69. doi Effect of a nickel-tolerant ACC deaminase-producing Pseudomonas strain on growth of nontransformed and transgenic canola plants
    Hilda Rodriguez
    Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada N2L 3G1
    Curr Microbiol 57:170-4. 2008
    ..The results suggest that this strain is a potential candidate to be used as an inoculant in both phytoremediation protocols and in plant growth promotion...
  70. doi Synergistic interactions between the ACC deaminase-producing bacterium Pseudomonas putida UW4 and the AM fungus Gigaspora rosea positively affect cucumber plant growth
    Elisa Gamalero
    Dipartimento di Scienze dell Ambiente e della Vita, Universita del Piemonte Orientale A Avogadro, Alessandria, Italy
    FEMS Microbiol Ecol 64:459-67. 2008
    ..Finally, the photosynthetic performance index was increased by the strain UW4 AcdS+ inoculated in combination with G. rosea BEG9. These results suggest a key role of this enzyme in the establishment and development of AM symbiosis...
  71. doi Anaerobic metabolism of phenol in proteobacteria and further studies of phenylphosphate carboxylase
    Sirko Schmeling
    Mikrobiologie, Fakultat fur Biologie, Universitat Freiburg, Schänzlestr 1, 79104, Freiburg, Germany
    Arch Microbiol 191:869-78. 2009
    ....
  72. doi Transcriptional regulation of ACC deaminase gene expression in Pseudomonas putida UW4
    Zhenyu Cheng
    Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, ON N2L3G1, Canada
    Can J Microbiol 54:128-36. 2008
    ..The data are interpreted in terms of a model in which AcdR and AcdB co-regulate the expression of the acdS gene...
  73. ncbi Plant growth-promoting trait of rhizobacteria isolated from soil contaminated with petroleum and heavy metals
    So Yeon Koo
    Department of Environmental Science and Engineering, Ewha Womans University, Seoul 120 750, Korea
    J Microbiol Biotechnol 20:587-93. 2010
    ..Clustering analysis by principal component analysis showed that RP was the most important factor influencing ecological distribution and physiological characterization of PGPP-possesing rhizobateria...
  74. pmc Identification and expression of benzylsuccinate synthase genes in a toluene-degrading methanogenic consortium
    Cheryl E Washer
    Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada M5S 3E5
    Appl Environ Microbiol 73:1367-9. 2007
    ..Transcription was upregulated with toluene but not with benzoate, consistent with the proposed function. These are the first bss sequences from a methanogenic culture...
  75. ncbi Thiamine pyrophosphate: an essential cofactor for the alpha-oxidation in mammals--implications for thiamine deficiencies?
    M Sniekers
    Departement Moleculaire Celbiologie, Afdeling Farmacologie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, O and N1, Herestraat 49, Box 601, 3000, Leuven, Belgium
    Cell Mol Life Sci 63:1553-63. 2006
    ..Whereas accumulation of phytanic acid and/or 2-hydroxyfatty acids or their alpha-oxidation intermediates in TRMA patients given a normal supply of thiamine is unlikely, this may not be true when malnourished...
  76. ncbi Toxigenic diversity of two different RAPD groups of Stachybotrys chartarum isolates analyzed by potential for trichothecene production and for boar sperm cell motility inhibition
    J Peltola
    Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, P O Box 56, FIN 00014, Finland
    Can J Microbiol 48:1017-29. 2002
    ..This indicates that the S. chartarum isolates belonging to different groups were genetically distinct in a much wider area than just the Tri5 gene...
  77. pmc Anaerobic toluene activation by benzylsuccinate synthase in a highly enriched methanogenic culture
    H R Beller
    Lawrence Livermore National Laboratory, Livermore, California 94551 0808, USA
    Appl Environ Microbiol 66:5503-5. 2000
    ..This is the first report of benzylsuccinate synthase activity in a methanogenic culture; the activity has previously been reported to occur in denitrifying, sulfate-reducing, and anoxygenic phototrophic bacteria...
  78. ncbi A novel, inducible, citral lyase purified from spores of Penicillium digitatum
    Wout A M Wolken
    Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen University, The Netherlands
    Eur J Biochem 269:5903-10. 2002
    ..6. The enzyme has a strong preference for the trans isomer of citral (geranial). Citral lyase also converts other alpha,beta-unsaturated aldehydes (farnesal, methyl-crotonaldehyde, decenal and cinnemaldehyde)...
  79. ncbi Unusual 4-hydroxybenzaldehyde synthase activity from tissue cultures of the vanilla orchid Vanilla planifolia
    Andrzej Podstolski
    Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
    Phytochemistry 61:611-20. 2002
    ..These forms appear to be interconvertible. The unusual properties of the 4HBS, and its appearance in different protein fractions, raise questions as to its physiological role in vanillin biosynthesis in vivo...
  80. ncbi Cloning and expression of sesquiterpene synthase genes from lettuce (Lactuca sativa L.)
    Mark H Bennett
    Department of Agricultural Sciences, Imperial College, Wye, Ashford, Kent TN25 5AH, UK
    Phytochemistry 60:255-61. 2002
    ..Southern hybridisation experiments showed that LTC1 and LTC2 were not part of a multigene family. The germacrene A synthases provide targets for modified expression to generate beneficial modifications to the SL profile in lettuce...
  81. ncbi X-ray crystal structures of D100E trichodiene synthase and its pyrophosphate complex reveal the basis for terpene product diversity
    Michael J Rynkiewicz
    Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6323, USA
    Biochemistry 41:1732-41. 2002
    ....
  82. ncbi Isolation and characterization of an unusual 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase gene from Enterobacter cloacae UW4
    J Li
    Department of Biology, University of Waterloo, Ontario, Canada
    Antonie Van Leeuwenhoek 80:255-61. 2001
    ..e., a deaminase that contains a mononuclear or binuclear metal center as compared to the canonical ACC deaminase which contains pyridoxal phosphate as a co-factor...
  83. ncbi [Effect of EGTA and La2+ on induction of sesquiterpene cyclase gene expression in leaves of Capsicum annuum by several abiotic elicitors]
    S He
    College of Crop Science, Fujian Agricultural University, Fuzhou 350002
    Ying Yong Sheng Tai Xue Bao 12:272-4. 2001
    ..annuum differed from that of biotic elicitors...
  84. pmc Structure of trichodiene synthase from Fusarium sporotrichioides provides mechanistic inferences on the terpene cyclization cascade
    M J Rynkiewicz
    Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323, USA
    Proc Natl Acad Sci U S A 98:13543-8. 2001
    ..This similarity indicates divergence from a common ancestor early in the evolution of terpene biosynthesis...
  85. pmc Benzylsuccinate synthase of Azoarcus sp. strain T: cloning, sequencing, transcriptional organization, and its role in anaerobic toluene and m-xylene mineralization
    G R Achong
    Environmental Engineering and Science, Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305-4020, USA
    J Bacteriol 183:6763-70. 2001
    ..The growth phenotype of the DeltabssA mutant could be rescued by reintroducing bssA in trans. These results demonstrate that benzylsuccinate synthase catalyzes the first step in anaerobic mineralization of both toluene and m-xylene...
  86. ncbi A stable organic free radical in anaerobic benzylsuccinate synthase of Azoarcus sp. strain T
    C J Krieger
    Department of Civil and Environmental Engineering, Environmental Engineering and Science, Stanford University, Stanford, California 94305, USA
    J Biol Chem 276:12924-7. 2001
    ..strain T benzylsuccinate synthase...
  87. ncbi cDNA cloning of sesquiterpene cyclase and squalene synthase, and expression of the genes in potato tuber infected with Phytophthora infestans
    H Yoshioka
    Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Japan
    Plant Cell Physiol 40:993-8. 1999
    ..On the other hand, wound-induced expression of the squalene synthase was suppressed in favor of the expression of vetispiradiene synthase regardless of inoculated races...
  88. ncbi Substrate range of benzylsuccinate synthase from Azoarcus sp. strain T
    H R Beller
    Department of Civil and Environmental Engineering, Stanford University, CA 94305 4020, USA
    FEMS Microbiol Lett 178:147-53. 1999
    ..The implications of these observations for the mechanism of benzylsuccinate synthase will be discussed...
  89. pmc Development and use of a reverse transcription-PCR assay to study expression of Tri5 by Fusarium species in vitro and in planta
    F M Doohan
    Cereals Department, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    Appl Environ Microbiol 65:3850-4. 1999
    ..culmorum, there were different levels of Tri5 expression in grain and chaff at later growth stages. We used the Tri5-specific primers to develop a PCR assay to detect trichothecene-producing Fusarium species in infected plant material...
  90. pmc Purification, molecular cloning, and expression of 2-hydroxyphytanoyl-CoA lyase, a peroxisomal thiamine pyrophosphate-dependent enzyme that catalyzes the carbon-carbon bond cleavage during alpha-oxidation of 3-methyl-branched fatty acids
    V Foulon
    Division of Pharmacology, Department of Molecular Cell Biology, Katholieke Universiteit Leuven, Campus Gasthuisberg B 3000 Leuven, Belgium
    Proc Natl Acad Sci U S A 96:10039-44. 1999
    ....
  91. ncbi Metabolic engineering to produce sesquiterpenes in yeast
    Beth E Jackson
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA
    Org Lett 5:1629-32. 2003
    ..This system allows sufficient production to characterize novel sesquiterpene synthase genes...
  92. pmc Residues C123 and D58 of the 2-methylisocitrate lyase (PrpB) enzyme of Salmonella enterica are essential for catalysis
    T L Grimek
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53726 4087, USA
    J Bacteriol 185:4837-43. 2003
    ..Based on the proposed mechanism of the closely related isocitrate lyases, PrpB residue C123 is proposed to serve as the active site base, and residue D58 is critical for the coordination of a required Mg(2+) ion...
  93. ncbi Substrate specificities and electron paramagnetic resonance properties of benzylsuccinate synthases in anaerobic toluene and m-xylene metabolism
    Knut Verfürth
    Mikrobiologie, Albert Ludwigs Universitat, Schanzlestrasse 1, Institut fur Biologie II, 79104 Freiburg, Germany
    Arch Microbiol 181:155-62. 2004
    ..The properties of the EPR signals are highly similar to those of the prototype glycyl radical enzyme pyruvate formate lyase, but differ slightly from previously reported parameters for partially purified benzylsuccinate synthase...
  94. ncbi Polyunsaturated fats attenuate the dietary phytol-induced increase in hepatic fatty acid oxidation in mice
    Toru Hashimoto
    Division of Food Functionality, National Food Reserach Institute, 2 1 12 Kannondai, Tsukuba 305 8642, Japan
    J Nutr 136:882-6. 2006
    ..We demonstrated that polyunsaturated fats attenuate the enhancing effect of dietary phytol on hepatic fatty acid oxidation. Dietary fat-dependent changes in the hepatic phytanic acid concentration cannot account for this phenomenon...
  95. ncbi Reaction mechanisms of the bacterial enzyme 1-aminocyclopropane-1-carboxylate deaminase
    Nikos Hontzeas
    Department of Pathology and Laboratory Medicine, UCLA David Geffen School of Medicine, 650 Charles E Young Drive South, Los Angeles, California 90095, USA
    Biotechnol Adv 24:420-6. 2006
    ..Decreased ethylene levels allow the plant to be more resistant to a wide variety of environmental stresses. Here the biochemical reaction mechanisms involved in ACC deaminase activity are critically reviewed...
  96. ncbi Two pockets in the active site of maize sesquiterpene synthase TPS4 carry out sequential parts of the reaction scheme resulting in multiple products
    Tobias G Köllner
    Max Planck Institute for Chemical Ecology, Hans Knoll Strasse 8, D 07745 Jena, Germany
    Arch Biochem Biophys 448:83-92. 2006
    ..Mutations in pocket II primarily inhibited the formation of bicylic compounds, suggesting that secondary cyclizations of the bisabolyl cation are catalyzed in pocket II...
  97. pmc Evidence for horizontal transfer of 1-aminocyclopropane-1-carboxylate deaminase genes
    N Hontzeas
    Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA
    Appl Environ Microbiol 71:7556-8. 2005
    ..The best maximum likelihood (ML) ACC deaminase tree was significantly more likely than four alternative ML trees, suggesting HGT...
  98. ncbi An unexpected diterpene cyclase from rice: functional identification of a stemodene synthase
    Dana Morrone
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Arch Biochem Biophys 448:133-40. 2006
    ....
  99. pmc Generation and phenotypic characterization of Aspergillus nidulans methylisocitrate lyase deletion mutants: methylisocitrate inhibits growth and conidiation
    Matthias Brock
    Institute for Microbiology, University Hannover, Herrenhauser Strasse 2, 30419 Hannover, Germany
    Appl Environ Microbiol 71:5465-75. 2005
    ..55 microM. Other isomers had no effect on enzymatic activity. Therefore, methylisocitrate was identified as a potential toxic compound for cellular metabolism...
  100. ncbi Evolutionary potential of (beta/alpha)8-barrels: in vitro enhancement of a "new" reaction in the enolase superfamily
    Jacob E Vick
    Department of Biochemistry, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    Biochemistry 44:11722-9. 2005
    ..These observations support our hypothesis that evolution of "new" functions in the enolase superfamily can occur simply by changes in specificity-determining residues...
  101. ncbi Modulation of plant ethylene levels by the bacterial enzyme ACC deaminase
    Bernard R Glick
    Department of Biology, University of Waterloo, Waterloo, Ont, Canada N2L 3G1
    FEMS Microbiol Lett 251:1-7. 2005
    ..Here, the biochemistry of ACC deaminase; the environmental distribution, regulation, evolution and expression of ACC deaminase genes; and information regarding the effect of this enzyme on different plants is documented and discussed...

Research Grants68

  1. MECHANISMS OF CARBON-CARBON LYASES AND KYNURENINASE
    Robert Phillips; Fiscal Year: 1993
    ..Also, we will prepare mutants of tryptophan indole-lyase by sitedirected mutagenesis. These studies will allow us to evaluate the structural and catalytic roles of specific amino acid residues...
  2. BIOSYNTHESIS OF MICROBIAL PRODUCTS
    DAVID CANE; Fiscal Year: 2000
    ..The investigators will carry out incubations to confirm the proposed role of these two proteins and to determine the cofactor requirements and mechanism of the oxidative ring-forming reaction. ..
  3. Biosynthesis of Microbial Isoprenoids
    David E Cane; Fiscal Year: 2010
    ....
  4. Biosynthesis of Microbial Polyketides
    DAVID CANE; Fiscal Year: 2009
    ..In addition, the results obtained will provide fundamental insights into how catalysis and molecular recognition control both substrate specificity and product molecular diversity in Nature. ..
  5. Biosynthesis of Microbial Polyketides
    DAVID CANE; Fiscal Year: 2007
    ..In addition, the results obtained will provide fundamental insights into how catalysis and molecular recognition control both substrate specificity and product molecular diversity in Nature. ..
  6. STEREOCHEMICAL STUDIES OF ISOPRENOID BIOSYNTHESIS
    DAVID CANE; Fiscal Year: 2007
    ..abstract_text> ..
  7. Biosynthesis of Microbial Polyketides
    DAVID CANE; Fiscal Year: 2005
    ..coli in order to study the EpoA-catalyzed conversion of malonyl-CoA to acetyl-S-EpoA, the substrate for the NRPS module EpoB. ..
  8. Biosynthesis of Microbial Polyketides
    David E Cane; Fiscal Year: 2010
    ..Finally, the proposed studies will lead to fundamental new biological insights into the central issue of modern molecular biochemistry, the relationship between protein sequence, structure, and function. ..
  9. BIOSYNTHESIS OF ANTIBIOTIC NATURAL PRODUCTS
    DAVID CANE; Fiscal Year: 1980
    ....
  10. BIOSYNTHESIS OF MICROBIAL NATURAL PRODUCTS
    DAVID CANE; Fiscal Year: 1993
    ..The PCR products will be inserted into suitable E. coli expression vectors and the resulting overexpressed proteins will be used by Prof. Spenser and his collaborators to examine the formation of pyridoxol...
  11. STEREOCHEMICAL STUDIES OF ISOPRENOID BIOSYNTHESIS
    DAVID CANE; Fiscal Year: 1993
    ..Finally, (- )-gamma-cadinene synthetase will be isolated from A. terreus and the mechanism of cyclization of farnesyl and nerolidyl pyrophosphate to a cis, trans-germacradienyl intermediate will be examined...
  12. BIOSYNTHESIS OF ANTIBIOTIC NATURAL PRODUCTS
    DAVID CANE; Fiscal Year: 1992
    ..The availability of cloned DNA will allow sequencing of the synthetase while the increased quantities of cyclase will be used for continued studies of the mechanism and stereochemistry of the cyclization reaction itself...
  13. MECHANISMS OF ENZYMATIC REACTIONS
    PERRY FREY; Fiscal Year: 2006
    ..Research on Fhit is directed toward elucidating its chemical reaction mechanism and the chemical basis for its activity as a tumor suppressor and its mechanistic relationships with galactose-1-P uridylyltransferase. ..
  14. ENZYME CATALYSIS OF ELECTRON AND GROUP TRANSFER
    PERRY FREY; Fiscal Year: 1990
    ..The reaction mechanism will be investigated by determining the tritium-kinetic isotope effect for the reaction of beta-[2-3H]lysine. The involvement of pridoxal phospate in this B12-like reaction will also be investigated...