ubiquitin conjugating enzymes

Summary

Summary: A class of enzymes that form a thioester bond to UBIQUITIN with the assistance of UBIQUITIN-ACTIVATING ENZYMES. They transfer ubiquitin to the LYSINE of a substrate protein with the assistance of UBIQUITIN-PROTEIN LIGASES.

Top Publications

  1. ncbi A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase
    Radmila Janjusevic
    Laboratory of Structural Microbiology, Rockefeller University, New York, NY 10021, USA
    Science 311:222-6. 2006
  2. ncbi Structure of a Shigella effector reveals a new class of ubiquitin ligases
    Yongqun Zhu
    National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China
    Nat Struct Mol Biol 15:1302-8. 2008
  3. ncbi Transient global cerebral ischemia induces a massive increase in protein sumoylation
    Wei Yang
    Department of Anesthesiology, Multidisciplinary Neuroprotection Laboratories, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Cereb Blood Flow Metab 28:269-79. 2008
  4. ncbi Quantitative proteomics reveals the function of unconventional ubiquitin chains in proteasomal degradation
    Ping Xu
    Department of Human Genetics, Center for Neurodegenerative Diseases, Emory University, Atlanta, GA 30322, USA
    Cell 137:133-45. 2009
  5. ncbi A proteolytic pathway that recognizes ubiquitin as a degradation signal
    E S Johnson
    Division of Biology, California Institute of Technology, Pasadena 91125, USA
    J Biol Chem 270:17442-56. 1995
  6. ncbi SUMO-targeted ubiquitin ligases in genome stability
    John Prudden
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 26:4089-101. 2007
  7. ncbi ER degradation of a misfolded luminal protein by the cytosolic ubiquitin-proteasome pathway
    M M Hiller
    Institut für Biochemie der Universität Stuttgart, Pfaffenwaldring 55, D 70569 Stuttgart, Germany
    Science 273:1725-8. 1996
  8. ncbi The Bur1/Bur2 complex is required for histone H2B monoubiquitination by Rad6/Bre1 and histone methylation by COMPASS
    Adam Wood
    Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Boulevard, St Louis, Missouri 63104, USA
    Mol Cell 20:589-99. 2005
  9. ncbi Control of spontaneous and damage-induced mutagenesis by SUMO and ubiquitin conjugation
    Philipp Stelter
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
    Nature 425:188-91. 2003
  10. ncbi Der3p/Hrd1p is required for endoplasmic reticulum-associated degradation of misfolded lumenal and integral membrane proteins
    J Bordallo
    Institut fur Biochemie, Universitat Stuttgart, Germany
    Mol Biol Cell 9:209-22. 1998

Research Grants

  1. MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSIS
    Per Olof Hasselgren; Fiscal Year: 2003
  2. Quantitative Analysis of RING E3 Ubiquitin Ligases
    Charles Brenner; Fiscal Year: 2009
  3. NUCLEAR HORMONE ACTION--ROLE OF UBIQUITIN PATHWAYS
    Zafar Nawaz; Fiscal Year: 2003
  4. Quantitative Analysis of RING E3 Ubiquitin Ligases
    CHARLES M BRENNER; Fiscal Year: 2010
  5. Structural Biology of U-box E3 Ubiquitin Ligases
    Walter J Chazin; Fiscal Year: 2010
  6. DISSECTION OF THE FUNCTIONS OF HERPES SIMPLEX VIRUS ICPO
    Bernard Roizman; Fiscal Year: 2007
  7. EXPRESSION OF CELL CYCLE PROTEINS IN BREAST CANCER
    Michele Pagano; Fiscal Year: 2000
  8. Identifying Ubiquitin E3 Substrates with Redesigned E3s
    Brian Kuhlman; Fiscal Year: 2005
  9. FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
    Arthur Haas; Fiscal Year: 2003
  10. Structural and Functional Characterization of BRCA1/BARD1
    Rachel Klevit; Fiscal Year: 2009

Detail Information

Publications178 found, 100 shown here

  1. ncbi A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase
    Radmila Janjusevic
    Laboratory of Structural Microbiology, Rockefeller University, New York, NY 10021, USA
    Science 311:222-6. 2006
    ..These results show that Pseudomonas syringae uses a mimic of host E3 ubiquitin ligases to inactivate plant defenses...
  2. ncbi Structure of a Shigella effector reveals a new class of ubiquitin ligases
    Yongqun Zhu
    National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China
    Nat Struct Mol Biol 15:1302-8. 2008
    ..Our analysis establishes a structurally and mechanistically distinct class of ubiquitin ligases found exclusively in pathogenic or symbiotic bacteria...
  3. ncbi Transient global cerebral ischemia induces a massive increase in protein sumoylation
    Wei Yang
    Department of Anesthesiology, Multidisciplinary Neuroprotection Laboratories, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Cereb Blood Flow Metab 28:269-79. 2008
    ....
  4. ncbi Quantitative proteomics reveals the function of unconventional ubiquitin chains in proteasomal degradation
    Ping Xu
    Department of Human Genetics, Center for Neurodegenerative Diseases, Emory University, Atlanta, GA 30322, USA
    Cell 137:133-45. 2009
    ..Ubc6 primarily synthesizes K11-linked chains, and K11 linkages function in the ERAD pathway. Thus, unconventional polyubiquitin chains are critical for ubiquitin-proteasome system function...
  5. ncbi A proteolytic pathway that recognizes ubiquitin as a degradation signal
    E S Johnson
    Division of Biology, California Institute of Technology, Pasadena 91125, USA
    J Biol Chem 270:17442-56. 1995
    ....
  6. ncbi SUMO-targeted ubiquitin ligases in genome stability
    John Prudden
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 26:4089-101. 2007
    ..The DNA repair factor Rad60 and its human homolog NIP45, which contain SLDs, are candidate STUbL targets. Consistently, Rad60 and Slx8-Rfp mutants have similar DNA repair defects...
  7. ncbi ER degradation of a misfolded luminal protein by the cytosolic ubiquitin-proteasome pathway
    M M Hiller
    Institut für Biochemie der Universität Stuttgart, Pfaffenwaldring 55, D 70569 Stuttgart, Germany
    Science 273:1725-8. 1996
    ..It is likely that CPY* entered the ER, was glycosylated, and was then transported back out of the ER lumen to the cytoplasmic side of the organelle, where it was conjugated with ubiquitin and degraded...
  8. ncbi The Bur1/Bur2 complex is required for histone H2B monoubiquitination by Rad6/Bre1 and histone methylation by COMPASS
    Adam Wood
    Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Boulevard, St Louis, Missouri 63104, USA
    Mol Cell 20:589-99. 2005
    ..Our results identify in vivo substrates for Bur1/Bur2, thus linking its role to transcriptional elongation and demonstrating a potential activation mechanism for histone H2B monoubiquitination by the Rad6/Bre1 complex...
  9. ncbi Control of spontaneous and damage-induced mutagenesis by SUMO and ubiquitin conjugation
    Philipp Stelter
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
    Nature 425:188-91. 2003
    ..Our findings assign a function to SUMO during S phase and demonstrate how ubiquitin and SUMO, by regulating the accuracy of replication and repair, contribute to overall genomic stability...
  10. ncbi Der3p/Hrd1p is required for endoplasmic reticulum-associated degradation of misfolded lumenal and integral membrane proteins
    J Bordallo
    Institut fur Biochemie, Universitat Stuttgart, Germany
    Mol Biol Cell 9:209-22. 1998
    ..Der3p might serve as a component programming the translocon for retrograde transport of ER proteins, or it might be involved in recognition through its lumenal RING-H2 motif of proteins of the ER that are destined for degradation...
  11. ncbi Ubiquitin-dependent proteolytic control of SUMO conjugates
    Kristina Uzunova
    Institute for Genetics, University of Cologne, Zulpicher Strasse 47, Cologne, Germany
    J Biol Chem 282:34167-75. 2007
    ..Simultaneous inhibition of both mechanisms leads to severe phenotypic defects...
  12. ncbi A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination
    Peter S Brzovic
    Department of Biochemistry, University of Washington, Seattle, 98195, USA
    Mol Cell 21:873-80. 2006
    ..Self-assembly has profound consequences for the processive formation of polyubiquitin (poly-Ub) chains in ubiquitination reactions directed by the breast and ovarian cancer tumor susceptibility protein BRCA1...
  13. ncbi RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells
    Jaehoon Kim
    The Rockefeller University, New York, NY 10065, USA
    Cell 137:459-71. 2009
    ..These studies establish the natural H2B ubiquitylation factors in human cells and also detail the mechanistic basis for H2B ubiquitylation and function during transcription...
  14. ncbi A conserved cysteine is essential for Pex4p-dependent ubiquitination of the peroxisomal import receptor Pex5p
    Chris Williams
    Department of Medical Biochemistry, Academic Medical Center, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    J Biol Chem 282:22534-43. 2007
    ..Together, our results strongly suggest that Pex4p-dependent ubiquitination of Pex5p occurs on a cysteine residue...
  15. ncbi Structural and functional conservation of error-free DNA postreplication repair in Schizosaccharomyces pombe
    Morgan Brown
    Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada
    DNA Repair (Amst) 1:869-80. 2002
    ..Hence, our data strongly support the hypothesis that the PRR function mediated by UBC13-MMS2 is conserved throughout eukaryotes...
  16. ncbi Opposing effects of ubiquitin conjugation and SUMO modification of PCNA on replicational bypass of DNA lesions in Saccharomyces cerevisiae
    Lajos Haracska
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1061, USA
    Mol Cell Biol 24:4267-74. 2004
    ....
  17. ncbi Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9
    M H Tatham
    Institute of Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews KY16 5ST, United Kingdom
    J Biol Chem 276:35368-74. 2001
    ..The ability to form polymeric chains is not shared by SUMO-1, and although all SUMO species use the same conjugation machinery, modification by SUMO-1 and SUMO-2/-3 may have distinct functional consequences...
  18. ncbi SUMOylation regulates Rad18-mediated template switch
    Dana Branzei
    IFOM, the FIRC Institute for Molecular Oncology Foundation, IFOM IEO Campus, Via Adamello 16, 20139 Milan, Italy
    Nature 456:915-20. 2008
    ..Altogether, our results unmask a role for PCNA ubiquitylation and SUMOylation pathways in promoting transient damage-induced replication-coupled recombination events involving sister chromatids at replication forks...
  19. ncbi SUMOylation regulates the transcriptional activity of JunB in T lymphocytes
    Johan Garaude
    Institut de Genetique Moleculaire de Montpellier, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 5535, 1919 Route de Mende, Montpellier Cedex 5, France
    J Immunol 180:5983-90. 2008
    ..Thus, our work demonstrates that sumoylation of JunB regulates its ability to induce cytokine gene transcription and likely plays a critical role in T cell activation...
  20. ncbi Human disorders of ubiquitination and proteasomal degradation
    Yong-Hui Jiang
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Curr Opin Pediatr 16:419-26. 2004
    ..Additional functional classes are likely to be defined, and individual disorders may involve multiple functional defects...
  21. ncbi An altered-specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair
    G Sebastiaan Winkler
    Department of Physiological Chemistry, University Medical Centre Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    J Mol Biol 337:157-65. 2004
    ..These are the first examples of altered-specificity E2-E3 enzyme pairs and give further insight into how E2-E3 specificity is obtained...
  22. ncbi Overexpression, genomic amplification and therapeutic potential of inhibiting the UbcH10 ubiquitin conjugase in human carcinomas of diverse anatomic origin
    Klaus W Wagner
    Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121, USA
    Oncogene 23:6621-9. 2004
    ..Together, these data demonstrate that UbcH10 plays an important role in tumor development and that its inhibition in combination with agonists of the TRAIL receptor may provide an enhanced therapeutic index...
  23. ncbi Determinants of functionality in the ubiquitin conjugating enzyme family
    Peter J Winn
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Structure 12:1563-74. 2004
    ..The specificities of only the ScUbc4/Ubc5 and ScUbc1p orthologs are reflected in their L4 and L7 loops...
  24. ncbi The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p
    Adam Wood
    Department of Biochemistry, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 278:34739-42. 2003
    ..Thus, in addition to its role during the elongation phase of transcription, the Paf1 complex appears to activate the function but not the placement of the Rad6-Bre1 ubiquitin-protein ligase at the promoters of active genes...
  25. ncbi Functional characterization of Rad18 domains for Rad6, ubiquitin, DNA binding and PCNA modification
    Valerie Notenboom
    Molecular Carcinogenesis and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nucleic Acids Res 35:5819-30. 2007
    ..Finally we find that the SAP domain, but not the zinc finger domain, is capable of DNA binding in vitro...
  26. ncbi Protein-protein interactions within an E2-RING finger complex. Implications for ubiquitin-dependent DNA damage repair
    Helle D Ulrich
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
    J Biol Chem 278:7051-8. 2003
    ..Finally, this study demonstrates that beyond its cooperation with the UBC13-MMS2 dimer, RAD5 must have an additional role in DNA damage repair independent of its RING finger domain...
  27. ncbi Identification and characterization of UEV3, a human cDNA with similarities to inactive E2 ubiquitin-conjugating enzymes
    Matthias Kloor
    Department of Molecular Pathology, University of Heidelberg, Im Neuenheimer Feld 220 221, Heidelberg, Germany
    Biochim Biophys Acta 1579:219-24. 2002
    ....
  28. ncbi RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO
    Carsten Hoege
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany
    Nature 419:135-41. 2002
    ..We demonstrate that these modifications differentially affect resistance to DNA damage, and that damage-induced PCNA ubiquitination is elementary for DNA repair and occurs at the same conserved residue in yeast and humans...
  29. ncbi Arabidopsis COP10 forms a complex with DDB1 and DET1 in vivo and enhances the activity of ubiquitin conjugating enzymes
    Yuki Yanagawa
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8104, USA
    Genes Dev 18:2172-81. 2004
    ..Thus, the CDD complex may act as a ubiquitylation-promoting factor to regulate photomorphogenesis...
  30. ncbi The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum
    S Fang
    Regulation of Protein Function Laboratory, Center for Cancer Research, National Cancer Institute, Building 10, Room 1B34, 9000 Rockville Pike, Bethesda, MD 20892 1152, USA
    Proc Natl Acad Sci U S A 98:14422-7. 2001
    ..gp78 has thus been found to be an example of a mammalian cellular E3 intrinsic to the ER, suggesting a potential link between ubiquitylation, ERAD, and metastasis...
  31. ncbi The E2-C vihar is required for the correct spatiotemporal proteolysis of cyclin B and itself undergoes cyclical degradation
    Endre Mathe
    Cancer Research UK Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
    Curr Biol 14:1723-33. 2004
    ..Substrate specificity and temporal activity of the APC/C has been thought to lie primarily with its two activators, Cdc20/Fizzy and Cdh1/Fizzy-related...
  32. ncbi Rpn4 is a physiological substrate of the Ubr2 ubiquitin ligase
    Li Wang
    Barbara Ann Karmanos Cancer Institute, Department of Pathology, Wayne State University School of Medicine, 110 Warren Avenue, Detroit, MI 48201, USA
    J Biol Chem 279:55218-23. 2004
    ..This study not only identified the ubiquitination apparatus for Rpn4 but also unveiled the first physiological substrate of Ubr2. The biological significance of Ubr2-mediated degradation of Rpn4 is also discussed...
  33. ncbi Structure of the anaphase-promoting complex/cyclosome interacting with a mitotic checkpoint complex
    Franz Herzog
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Science 323:1477-81. 2009
    ..These observations clarify the structural basis for the inhibition of APC/C by spindle checkpoint proteins...
  34. ncbi UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit
    Mathew J Garnett
    University of Cambridge, Department of Oncology and The Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge, CB2 OXZ, UK
    Nat Cell Biol 11:1363-9. 2009
    ..Thus, UBE2S functions with the APC/C in a two-step mechanism to control substrate ubiquitylation that is essential for mitotic exit after prolonged SAC activation, providing a new model for APC/C function in human cells...
  35. ncbi Increased phosphorylation and dimethylation of XY body histones in the Hr6b-knockout mouse is associated with derepression of the X chromosome
    Willy M Baarends
    Department of Reproduction and Development, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    J Cell Sci 120:1841-51. 2007
    ..We conclude that HR6B exerts control over different histone modifications in spermatocytes and spermatids, and that this function contributes to the postmeiotic maintenance of X chromosome silencing...
  36. ncbi Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen
    Ildiko Unk
    Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H 6726 Szeged, Hungary
    Proc Natl Acad Sci U S A 103:18107-12. 2006
    ....
  37. ncbi Regulation of p53 localization and activity by Ubc13
    Aaron Laine
    Signal Transduction Program, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell Biol 26:8901-13. 2006
    ..Our findings point to a newly discerned mechanism important in the regulation of p53 organization, localization, and activity by Ubc13...
  38. ncbi Mms2-Ubc13-dependent and -independent roles of Rad5 ubiquitin ligase in postreplication repair and translesion DNA synthesis in Saccharomyces cerevisiae
    Venkateswarlu Gangavarapu
    Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, Galveston, TX 77555 1061
    Mol Cell Biol 26:7783-90. 2006
    ..In contrast to the role of Rad5 in PRR, however, where its function is coupled with that of Mms2-Ubc13, Rad5 function in TLS would be largely independent of this ubiquitin-conjugating enzyme complex...
  39. ncbi Ubiquitylation of yeast proliferating cell nuclear antigen and its implications for translesion DNA synthesis
    Lajos Haracska
    Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H 6701 Szeged, Hungary
    Proc Natl Acad Sci U S A 103:6477-82. 2006
    ..These observations lead us to suggest a role for PCNA monoubiquitylation in disrupting the PCNA binding of a protein(s) that otherwise is inhibitory to the binding of PCNA by TLS Pols...
  40. ncbi Structure and interactions of the helical and U-box domains of CHIP, the C terminus of HSP70 interacting protein
    Zhen Xu
    Department of Molecular Cardiology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Biochemistry 45:4749-59. 2006
    ..Our results provide insights into conformational variability in the domain arrangement of CHIP and into U-box-mediated recruitment of UbcH5b for the ubiquitination of Hsp70 and Hsp90 substrates...
  41. ncbi UbcH10 overexpression may represent a marker of anaplastic thyroid carcinomas
    P Pallante
    Dipartimento di Biologia e Patologia Cellulare e Molecolare c o Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, Facoltà di Medicina e Chirurgia di Napoli, Universita degli Studi di Napoli Federico II, Italy
    Br J Cancer 93:464-71. 2005
    ..Taken together, these results would indicate that UbcH10 overexpression is involved in thyroid cell proliferation, and may represent a marker of thyroid anaplastic carcinomas...
  42. ncbi Regulation of Smurf2 ubiquitin ligase activity by anchoring the E2 to the HECT domain
    Abiodun A Ogunjimi
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Mol Cell 19:297-308. 2005
    ..Thus, E2 enzyme recognition by an E3 HECT enzyme is not constitutively competent and provides a point of control for regulating the ubiquitin ligase activity through the action of auxiliary proteins...
  43. ncbi UBE2V2 (MMS2) is not required for effective immunoglobulin gene conversion or DNA damage tolerance in DT40
    Laura J Simpson
    MRC Laboratory of Molecular Biology, Division of Protein and Nucleic Acid Chemistry, Hills Road, Cambridge, CB2 2QH, UK
    DNA Repair (Amst) 4:503-10. 2005
    ..Together these data suggest that signalling through PCNA ubiquitination is not required for immunoglobulin diversification in DT40...
  44. ncbi TEB4 is a C4HC3 RING finger-containing ubiquitin ligase of the endoplasmic reticulum
    Gerco Hassink
    Department of Medical Microbiology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Biochem J 388:647-55. 2005
    ..These properties are reminiscent of E3 enzymes, which are involved in ER-associated protein degradation. TEB4 is an ER degradation substrate itself, promoting its own degradation in a RING finger- and proteasome-dependent manner...
  45. ncbi Histone H2B ubiquitylation is associated with elongating RNA polymerase II
    Tiaojiang Xiao
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, 405 Mary Ellen Jones Bldg, Chapel Hill, NC 27599-7260, USA
    Mol Cell Biol 25:637-51. 2005
    ..Collectively, our results indicate a role for Rad6 and H2B ubiquitylation during the elongation cycle of transcription and suggest a mechanism by which H3 methylation may be regulated...
  46. ncbi Solution structure of the ubiquitin-conjugating enzyme UbcH5B
    Klaartje Houben
    Department of NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Mol Biol 344:513-26. 2004
    ..The possible implication of this conformational freedom of Asn77 for its catalytic function is discussed...
  47. ncbi Synthesis of free and proliferating cell nuclear antigen-bound polyubiquitin chains by the RING E3 ubiquitin ligase Rad5
    Candice M Carlile
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 284:29326-34. 2009
    ..These findings indicate that Rad5-mediated recognition of monoUb-PCNA in vivo is likely to depend upon interactions with additional factors at stalled replication forks...
  48. ncbi Structure of a c-Cbl-UbcH7 complex: RING domain function in ubiquitin-protein ligases
    N Zheng
    Cellular Biochemistry and Biophysics Program, Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cell 102:533-9. 2000
    ....
  49. ncbi The Saccharomyces cerevisiae RAD30 gene, a homologue of Escherichia coli dinB and umuC, is DNA damage inducible and functions in a novel error-free postreplication repair mechanism
    J P McDonald
    Section on DNA Replication, Repair and Mutagenesis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2725, USA
    Genetics 147:1557-68. 1997
    ..These findings suggest that RD30 participates in a novel error-free repair pathway dependent on RAD6 and RAD18, but independent of REV1, REV3, REV7 and RAD5...
  50. ncbi Role of UEV-1, an inactive variant of the E2 ubiquitin-conjugating enzymes, in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells
    E Sancho
    Departamento de Biologia Molecular, Instituto de Biología del Cáncer, IMIM CSIC, Barcelona, Spain
    Mol Cell Biol 18:576-89. 1998
    ..This was accompanied with a profound inhibition of the mitotic kinase, cdk1. These results suggest that UEV proteins are involved in the control of differentiation and could exert their effects by altering cell cycle distribution...
  51. ncbi Identification, expression, and chromosomal localization of ubiquitin conjugating enzyme 7 (UBE2G2), a human homologue of the Saccharomyces cerevisiae ubc7 gene
    N Katsanis
    Department of Neurogenetics, Imperial College School of Medicine at St Mary s, Norfolk Place, London, W2 1PG, United Kingdom
    Genomics 51:128-31. 1998
    ..is an essential step in the proteasome-dependent degradation process and is mediated by a family of ubiquitin conjugating enzymes (UBC). Several of these have been identified in a variety of organisms...
  52. ncbi Rad6-dependent ubiquitination of histone H2B in yeast
    K Robzyk
    Program in Molecular Biology, Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Science 287:501-4. 2000
    ..uH2B was not detected in rad6 mutants, which are defective for the ubiquitin-conjugating enzyme Ubc2, thus identifying Rad6 as the major cellular activity that ubiquitinates H2B in yeast...
  53. ncbi The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53
    M Scheffner
    Laboratory of Tumor Virus Biology, National Cancer Institute, Bethesda, Maryland 20892
    Cell 75:495-505. 1993
    ..The ubiquitination of p53 requires the E1 enzyme and a novel E2 in mammalian cells, while E3 activity is conferred by the E6-E6-AP complex. Furthermore, E6-AP appears to have ubiquitin-protein ligase activity in the absence of E6...
  54. ncbi Human ubiquitin-protein ligase Nedd4: expression, subcellular localization and selective interaction with ubiquitin-conjugating enzymes
    T Anan
    Department of Tumor Genetics and Biology, Kumamoto University School of Medicine, Japan
    Genes Cells 3:751-63. 1998
    ..Nedd4 has been reported to be involved in the selective ubiquitination of some regulatory proteins in transcription and membrane transport...
  55. ncbi Specific complex formation between yeast RAD6 and RAD18 proteins: a potential mechanism for targeting RAD6 ubiquitin-conjugating activity to DNA damage sites
    V Bailly
    Department of Biophysics, University of Rochester, New York 14642
    Genes Dev 8:811-20. 1994
    ..We also show that RAD6 forms separate complexes with RAD18 and with UBR1, and the extremely conserved amino terminus of RAD6 that is required for complex formation with UBR1 is dispensable for complex formation with RAD18...
  56. ncbi Identification of a family of noncanonical ubiquitin-conjugating enzymes structurally related to yeast UBC6
    D Lester
    Bone Biology Group, Roslin Institute, Roslin, Scotland, EH25 9PS, United Kingdom
    Biochem Biophys Res Commun 269:474-80. 2000
    ..By analogy with yeast UBC6 they are likely to be localised to the endoplasmic reticulum where they may be involved in targeting retrotranslocated, ER-associated proteins for proteasomal degradation...
  57. ncbi Role of Cue1p in ubiquitination and degradation at the ER surface
    T Biederer
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13122 Berlin, Germany
    Science 278:1806-9. 1997
    ....
  58. ncbi Human Cdc34 and Rad6B ubiquitin-conjugating enzymes target repressors of cyclic AMP-induced transcription for proteolysis
    D Pati
    Texas Children s Cancer Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 19:5001-13. 1999
    ....
  59. ncbi Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2-hUbc13
    T F Moraes
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
    Nat Struct Biol 8:669-73. 2001
    ..The structure of the hMms2-hUbc13 complex provides the conceptual foundation for understanding the mechanism of Lys 63 multiubiquitin chain assembly and for its interactions with the RING finger proteins Rad5 and Traf6...
  60. ncbi Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MAT alpha 2 repressor
    P Chen
    Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637
    Cell 74:357-69. 1993
    ..These data reveal an unexpected overlap in substrate specificity among diverse UBC enzymes and suggest a combinatorial mechanism of substrate selection in which UBC enzymes partition into multiple ubiquitination complexes...
  61. ncbi Yeast DNA repair proteins Rad6 and Rad18 form a heterodimer that has ubiquitin conjugating, DNA binding, and ATP hydrolytic activities
    V Bailly
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555 1061, USA
    J Biol Chem 272:23360-5. 1997
    ....
  62. ncbi The Schizosaccharomyces pombe hus5 gene encodes a ubiquitin conjugating enzyme required for normal mitosis
    F al-Khodairy
    MRC Cell Mutation Unit, Sussex University, UK
    J Cell Sci 108:475-86. 1995
    ..We have cloned and sequenced the hus5+ gene. It is a novel member of the E2 family of ubiquitin conjugating enzymes (UBCs)...
  63. ncbi Sequence determinants of E2-E6AP binding affinity and specificity
    Ziad M Eletr
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    J Mol Biol 369:419-28. 2007
    ..This result indicates that E2-E3 binding specificities are a function of both favorable interactions that promote binding, and unfavorable interactions that prevent binding with unwanted partners...
  64. ncbi SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage
    Y Mao
    Department of Pharmacology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 97:4046-51. 2000
    ..iii) TOP1 physically interacts with UBC9. (iv) Ubc9 mutant yeast cells expressing human DNA TOP1 was hypersensitive to CPT, suggesting that UBC9/SUMO-1 may be involved in the repair of TOP1-mediated DNA damage...
  65. ncbi Modulation of Ubc4p/Ubc5p-mediated stress responses by the RING-finger-dependent ubiquitin-protein ligase Not4p in Saccharomyces cerevisiae
    Klaas W Mulder
    Department of Physiological Chemistry, University Medical Centre Utrecht, Utrecht, The Netherlands
    Genetics 176:181-92. 2007
    ..Together, our results show that Not4p functions as an E3 ligase by modulating Ubc4p/Ubc5p-mediated stress responses in vivo...
  66. ncbi Human HRD1 is an E3 ubiquitin ligase involved in degradation of proteins from the endoplasmic reticulum
    Marjolein Kikkert
    Department of Medical Microbiology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    J Biol Chem 279:3525-34. 2004
    ..Additionally we show that human HRD1 is involved in the elimination of two model ER-associated degradation substrates, TCR-alpha and CD3-delta...
  67. ncbi Genome-wide analysis identifies MYND-domain protein Mub1 as an essential factor for Rpn4 ubiquitylation
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, 110 E Warren Ave, Detroit, MI 48201, USA
    Mol Cell Biol 28:1404-12. 2008
    ..Together, these data suggest that Mub1 and Ubr2 cooperate to transfer ubiquitin to Rpn4 from Rad6 and that Mub1 may switch from a partner to a substrate of the Ubr2/Rad6 ubiquitin ligase...
  68. ncbi Human HLTF functions as a ubiquitin ligase for proliferating cell nuclear antigen polyubiquitination
    Ildiko Unk
    Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Temesvari korut 62, H 6726, Szeged, Hungary
    Proc Natl Acad Sci U S A 105:3768-73. 2008
    ..A requirement of HLTF for error-free postreplication repair of damaged DNA is in keeping with its cancer-suppression role...
  69. ncbi Solution conformation of Lys63-linked di-ubiquitin chain provides clues to functional diversity of polyubiquitin signaling
    Ranjani Varadan
    Department of Chemistry and Biochemistry, Center of Biomolecular Structure and Organization, University of Maryland, College Park, Maryland 20742, USA
    J Biol Chem 279:7055-63. 2004
    ..In contrast, Lys48-linked di-ubiquitin binds in a different, higher affinity mode that has yet to be determined. This is the first experimental evidence that alternatively linked polyubiquitin chains adopt distinct conformations...
  70. ncbi Role of an N-terminal site of Ubc9 in SUMO-1, -2, and -3 binding and conjugation
    Michael H Tatham
    Center for Biomolecular Sciences, University of St Andrews, St Andrews, Scotland
    Biochemistry 42:9959-69. 2003
    ..The conservation of E2 enzymes across the ubiquitin and ubiquitin-like protein pathways indicates that analogous N-terminal sites of E2 enzymes are likely to have similar roles in general...
  71. ncbi BRCA1-independent ubiquitination of FANCD2
    Cassandra J Vandenberg
    Protein and Nucleic Acid Chemistry Division, MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 12:247-54. 2003
    ..Consequently, while BRCA1 affects the accumulation of FANCD2 at sites of DNA damage, BRCA1/BARD1 E3 ligase activity is not essential for the monoubiquitination of FANCD2...
  72. ncbi RNF5, a RING finger protein that regulates cell motility by targeting paxillin ubiquitination and altered localization
    Christine Didier
    Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, NY 10029, USA
    Mol Cell Biol 23:5331-45. 2003
    ..Concomitantly, RNF5 expression results in inhibition of cell motility. Via targeting of paxillin ubiquitination, which alters its localization, RNF5 emerges as a novel regulator of cell motility...
  73. ncbi The herpes simplex virus type 1 (HSV-1) regulatory protein ICP0 interacts with and Ubiquitinates p53
    Chris Boutell
    Medical Research Council Virology Unit, Glasgow G11 5JR, Scotland, United Kingdom
    J Biol Chem 278:36596-602. 2003
    ..Ubiquitination of p53 requires ICP0 to have an intact RING finger domain and occurs independently of its ability to bind to the ubiquitin-specific protease USP7...
  74. ncbi The Png1-Rad23 complex regulates glycoprotein turnover
    Ikjin Kim
    Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78245, USA
    J Cell Biol 172:211-9. 2006
    ..We propose that the substrate specificity of Rad23 and other Ub binding proteins is determined by their interactions with various cofactors involved in specific degradation pathways...
  75. ncbi Transcriptional activity of peroxisome proliferator-activated receptor gamma is modulated by SUMO-1 modification
    Takayuki Ohshima
    Department of Viral Oncology, Institute for Virus Research, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan
    J Biol Chem 279:29551-7. 2004
    ..These results suggest that the PPARgamma-dependent transactivation pathway seems to be modulated by SUMO-1 modification and may serve as a novel target for apoptosis-induction therapy in cancer cells...
  76. ncbi Novel interaction between Apc5p and Rsp5p in an intracellular signaling pathway in Saccharomyces cerevisiae
    Terra G Arnason
    Department of Medicine, Royal University Hospital, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    Eukaryot Cell 4:134-46. 2005
    ..Therefore, the antagonistic interplay between Deltaubc7 and rsp5CA, with respect to cdc34-2 and apc5CA, indicates that the outcome of Rsp5p's interaction with Cdc34p and Apc5p may depend on the E2 interacting with Rsp5p...
  77. ncbi Mechanistic insight into the allosteric activation of a ubiquitin-conjugating enzyme by RING-type ubiquitin ligases
    Engin Ozkan
    Department of Biochemistry and Pharmacology and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 102:18890-5. 2005
    ..Our studies reveal structural determinants for communication between distal functional sites of E2s and suggest that RING-type E3s activate E2s allosterically...
  78. ncbi Different HECT domain ubiquitin ligases employ distinct mechanisms of polyubiquitin chain synthesis
    Min Wang
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    EMBO J 24:4324-33. 2005
    ..A small region near the N-terminus of the conserved HECT domain helps to bring about this functional distinction. Thus, a given HECT domain can specify both the linkage of a polyubiquitin chain and the mechanism of its assembly...
  79. ncbi Phosphorelay-regulated degradation of the yeast Ssk1p response regulator by the ubiquitin-proteasome system
    Naoto Sato
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Mol Cell Biol 23:6662-71. 2003
    ..Our results indicate that unphosphorylated Ssk1p is selectively degraded by the Ubc7p-dependent ubiquitin-proteasome system and that this mechanism downregulates the HOG pathway after the completion of the osmotic adaptation...
  80. ncbi Regulation of the cytoplasmic quality control protein degradation pathway by BAG2
    Qian Dai
    Carolina Cardiovascular Biology Center and the Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina 27599 7126, USA
    J Biol Chem 280:38673-81. 2005
    ..The association of BAG2 with CHIP provides a cochaperone-dependent regulatory mechanism for preventing unregulated ubiquitylation of misfolded proteins by CHIP...
  81. ncbi DNA repair: right on target with ubiquitin
    Cecile M Pickart
    Nature 419:120-1. 2002
  82. ncbi Human MMS21/NSE2 is a SUMO ligase required for DNA repair
    Patrick Ryan Potts
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, 75390 9041, USA
    Mol Cell Biol 25:7021-32. 2005
    ..Our findings suggest that the human SMC5/6 complex and the SUMO ligase activity of hMMS21 are required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair in human cells...
  83. ncbi Physical interaction between specific E2 and Hect E3 enzymes determines functional cooperativity
    S Kumar
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 272:13548-54. 1997
    ....
  84. ncbi Distinct functional domains of Ubc9 dictate cell survival and resistance to genotoxic stress
    Robert C A M van Waardenburg
    Dept of Molecular Pharmacology, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105, USA
    Mol Cell Biol 26:4958-69. 2006
    ..Our findings establish a functional interaction between N-terminal and substrate-binding domains of Ubc9 and distinguish the activities of E3 ligases Siz1 and Siz2 in regulating cellular responses to genotoxic stress...
  85. ncbi Have you HRD? Understanding ERAD is DOAble!
    Nurzian Ismail
    Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore 117604
    Cell 126:237-9. 2006
    ..2006) and Carvalho et al. (2006) report that two distinct protein complexes at the endoplasmic reticulum membrane are responsible for the recognition and degradation of specific subsets of protein substrates...
  86. ncbi Sumoylation and proteasomal activity determine the transactivation properties of the mineralocorticoid receptor
    M Tirard
    Max Planck Institute of Psychiatry, 80804 Munich, Germany
    Mol Cell Endocrinol 268:20-9. 2007
    ..The data suggest that the overall transcriptional activity of MR can be modulated by its sumoylation potential as well as the sumoylation level of MR-interacting proteins, and requires the continuous function of the proteasome...
  87. ncbi The E2-E3 interaction in the N-end rule pathway: the RING-H2 finger of E3 is required for the synthesis of multiubiquitin chain
    Y Xie
    Division of Biology, 147 75, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    EMBO J 18:6832-44. 1999
    ..These results defined the topography of the Ubc2p-Ubr1p interaction and revealed the essential function of the RING-H2 finger, a domain that is present in many otherwise dissimilar E3 proteins of the ubiquitin system...
  88. ncbi Topors functions as an E3 ubiquitin ligase with specific E2 enzymes and ubiquitinates p53
    Rajeev Rajendra
    Department of Pharmacology, The Cancer Institute of New Jersey, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08901, USA
    J Biol Chem 279:36440-4. 2004
    ..These results are similar to the recent finding that a Drosophila topors orthologue ubiquitinates the Hairy transcriptional repressor and suggest that topors functions as a ubiquitin ligase for multiple transcription factors...
  89. ncbi The C-terminal region of an Apg7p/Cvt2p is required for homodimerization and is essential for its E1 activity and E1-E2 complex formation
    M Komatsu
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo 113 8421, Japan
    J Biol Chem 276:9846-54. 2001
    ..These results suggest that Apg7p forms a homodimer via the C-terminal region and that the C-terminal region is essential for both the activity of the E1 enzyme for Apg12p and Apg8p as well as the formation of an E1-E2 complex for Apg8p...
  90. ncbi The Nedd8-conjugated ROC1-CUL1 core ubiquitin ligase utilizes Nedd8 charged surface residues for efficient polyubiquitin chain assembly catalyzed by Cdc34
    Kenneth Wu
    Derald H Ruttenberg Cancer Center, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 277:516-27. 2002
    ..These results suggest that Nedd8 charged surface residues mediate the activation of ROC1-CUL1 to specifically support Cdc34-catalyzed ubiquitin polymerization...
  91. ncbi On the road to repair: PCNA encounters SUMO and ubiquitin modifications
    Michael J Matunis
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Mol Cell 10:441-2. 2002
    ..Now, ubiquitin and SUMO modification of proliferating cell nuclear antigen (PCNA) is shown to be induced by DNA damage and linked to components of the RAD6 pathway...
  92. ncbi Roles of mouse UBC13 in DNA postreplication repair and Lys63-linked ubiquitination
    Carolyn Ashley
    Department of Microbiology and Immunology, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Sask S7N 5E5, Canada
    Gene 285:183-91. 2002
    ..These results suggest that the roles of UBC13 are conserved throughout eukaryotes and that the mouse is an appropriate model for the study of Ubc13-mediated Lys63-linked ubiquitin signaling pathways in humans...
  93. ncbi Sprouty2 attenuates epidermal growth factor receptor ubiquitylation and endocytosis, and consequently enhances Ras/ERK signalling
    Esther Sook Miin Wong
    Signal Transduction Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, 30 Medical Drive, Singapore 117609
    EMBO J 21:4796-808. 2002
    ..We conclude that hSpry2 potentiates EGFR signalling by specifically intercepting c-Cbl-mediated effects on receptor down-regulation...
  94. ncbi Mcm3 is polyubiquitinated during mitosis before establishment of the pre-replication complex
    Irene H Cheng
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853 2703, USA
    J Biol Chem 277:41706-14. 2002
    ..Possible roles for ubiquitinated Mcm3p in the assembly of the MCM complex at replication origins are discussed...
  95. ncbi Pex18p is constitutively degraded during peroxisome biogenesis
    P E Purdue
    Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, 1190 Fifth Ave, New York, NY 10029 6574, USA
    J Biol Chem 276:47684-9. 2001
    ..These data represent, to the best of our knowledge, the first instance of an organelle biogenesis factor that is degraded constitutively and rapidly...
  96. ncbi An HRD/DER-independent ER quality control mechanism involves Rsp5p-dependent ubiquitination and ER-Golgi transport
    Cole M Haynes
    University of Missouri Kansas City, Division of Cell Biology and Biophysics, School of Biological Sciences, Kansas City, MO 64110, USA
    J Cell Biol 158:91-101. 2002
    ..Both the HIP and HRD/DER pathways contribute to the degradation of CPY*, and only by eliminating both is CPY* degradation completely blocked...
  97. ncbi A role for mammalian Ubc6 homologues in ER-associated protein degradation
    Uwe Lenk
    The Max Delbrück Centrum für Molekulare Medizin, Robert Rossle Str 10, 13092 Berlin, Germany
    J Cell Sci 115:3007-14. 2002
    ..Similarly, we describe that the expression level of Ubc6p in yeast is also critical for ERAD, suggesting that the Ubc6p function is highly conserved from yeast to mammals...
  98. ncbi Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex
    Ning Zheng
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York 10021, USA
    Nature 416:703-9. 2002
    ..The structure suggests that Cul1 may contribute to catalysis through the positioning of the substrate and the ubiquitin-conjugating enzyme, and this model is supported by Cul1 mutations designed to eliminate the rigidity of the scaffold...
  99. ncbi A novel factor required for the SUMO1/Smt3 conjugation of yeast septins
    Y Takahashi
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Gene 275:223-31. 2001
    ..A conserved cysteine residue in the domain was essential for this conjugation. Furthermore, Siz1 was localized at the mother-bud neck in the M-phase and physically bound to both E2 and the target proteins...
  100. ncbi Arabidopsis COP10 is a ubiquitin-conjugating enzyme variant that acts together with COP1 and the COP9 signalosome in repressing photomorphogenesis
    Genki Suzuki
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Genes Dev 16:554-9. 2002
    ..Our data indicates that COP10 defines a possible E2 activity, thus validating the working hypothesis that the pleiotropic COP/DET/FUS group of proteins defined a protein ubiquitination pathway...
  101. ncbi Regulatory functions of ubiquitination in the immune system
    Yinon Ben-Neriah
    The Lautenberg Center for Immunology, The Hebrew University Hadassah Medical School, Jerusalem, 91120, Israel
    Nat Immunol 3:20-6. 2002
    ..Therefore, it is not surprising that the ever-evolving immune system is an excellent mirror for the multiple roles played by ubiquitination within an organism...

Research Grants69

  1. MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSIS
    Per Olof Hasselgren; Fiscal Year: 2003
    ..e., inhibition of myofilament release by treatment with a calcium antagonist and inhibition of ubiquitin/proteasome-dependent degradation of the released myofilaments by a proteasome blocker. ..
  2. Quantitative Analysis of RING E3 Ubiquitin Ligases
    Charles Brenner; Fiscal Year: 2009
    ..2003; Kirkpatrick et al., 2005a). Third, the E2 ubiquitin conjugating enzymes have different specificities and potentially form different sets of target protein and polyubiquitin ..
  3. NUCLEAR HORMONE ACTION--ROLE OF UBIQUITIN PATHWAYS
    Zafar Nawaz; Fiscal Year: 2003
    ..Of the NEDD8 pathway as a co-activator of NHRs and to characterize this role in nuclear hormone receptor co-activation. ..
  4. Quantitative Analysis of RING E3 Ubiquitin Ligases
    CHARLES M BRENNER; Fiscal Year: 2010
    ..2003;Kirkpatrick et al., 2005a). Third, the E2 ubiquitin conjugating enzymes have different specificities and potentially form different sets of target protein and polyubiquitin ..
  5. Structural Biology of U-box E3 Ubiquitin Ligases
    Walter J Chazin; Fiscal Year: 2010
    ..Despite the availability of structures of CHIP, E2 ubiquitin conjugating enzymes and heat shock proteins, the factors controlling the selection of ubiquitination sites on the ..
  6. DISSECTION OF THE FUNCTIONS OF HERPES SIMPLEX VIRUS ICPO
    Bernard Roizman; Fiscal Year: 2007
    ..We expect that the proposed studies will help understand the role of ICP0 in the evolution of diseases caused by HSV-1 and HSV-2. ..
  7. EXPRESSION OF CELL CYCLE PROTEINS IN BREAST CANCER
    Michele Pagano; Fiscal Year: 2000
    ..in breast carcinomas, they will analyze tumor breast samples for the abundance of specific degradation activities and for the levels of various ubiquitin conjugating enzymes (Ubcs) involved in cell cycle regulation (Specific Aim 2).
  8. Identifying Ubiquitin E3 Substrates with Redesigned E3s
    Brian Kuhlman; Fiscal Year: 2005
    ..Favorable results will indicate that this new method should be an excellent approach for identifying substrates of other E3 ubiquitin ligases important to health and disease. ..
  9. FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
    Arthur Haas; Fiscal Year: 2003
    ..kDa polypeptides bear remarkable yet cryptic sequence similarity to the conserved catalytic domain of ubiquitin conjugating enzymes (Ubc). The present proposal comprises three specific aims...
  10. Structural and Functional Characterization of BRCA1/BARD1
    Rachel Klevit; Fiscal Year: 2009
    ..A full description of the molecular interactions that are critical to BRCA1 function will provide new insight into the early events associated with loss of BRCA1 that lead to tumorogenesis. ..
  11. Structural and Functional Characterization of BRCA1/BARD1
    Rachel E Klevit; Fiscal Year: 2010
    ..A full description of the molecular interactions that are critical to BRCA1 function will provide new insight into the early events associated with loss of BRCA1 that lead to tumorogenesis. ..
  12. Functional Anatomy of the Cul3 Ubiquitin Ligase
    Jeffrey Harper; Fiscal Year: 2007
    The SCF ubiquitin ligase pathway employs cullin proteins to assemble substrate specificity modules and ubiquitin conjugating enzymes, thereby promoting substrate ubiquitination...
  13. NOVEL DEUBIQUITINATING ENZYME, UBP46, IN HEMATOPOIESIS
    Dong Er Zhang; Fiscal Year: 2002
    ..Two major groups of enzymes, ubiquitin conjugating enzymes and deubiquitinating enzymes, regulate the balance of protein ubiquitination...
  14. Analysis of ubiquitination enzymes in C. elegans
    Lynn Boyd; Fiscal Year: 2005
    ..typically achieved via a three-step pathway utilizing the E1 ubiquitin activating enzyme (E1), the E2 ubiquitin conjugating enzymes (Ubc), and the E3 ubiquitin ligases (E3)...
  15. Immune evasion by gamma 2 Herpesviruses
    KLAUS J FRUEH; Fiscal Year: 2010
    ..abstract_text> ..
  16. Hematopoiesis by Ubiquitination and Deubiquitination
    ALAN D ANDREA; Fiscal Year: 2005
    ..Protein ubiquitination is controlled by the coordinate action of ubiquitin conjugating enzymes and deubiquitinating enzymes...
  17. ATP-UBIQUITIN-DEPENDENT PROTEOLYSIS
    Arthur Haas; Fiscal Year: 2006
    ..Overall, the proposed studies seek to apply conventional biochemical and genetic methods to the detailed examination of key processes within ubiquitin-dependent 26S proteasome targeting at the molecular level. ..
  18. ATP-UBIQUITIN-DEPENDENT PROTEOLYSIS
    Arthur Haas; Fiscal Year: 2003
    ..Overall, the proposed studies seek to apply conventional biochemical and genetic methods to the detailed examination of key processes within ubiquitin-dependent 26S proteasome targeting at the molecular level. ..
  19. MOLECULAR CONTROL OF CELL PROLIFERATION
    DAVID OWEN MORGAN; Fiscal Year: 2010
    ..These studies are also likely to illuminate general mechanisms of protein ubiquitination, a regulatory modification of importance throughout cell biology and human disease. ..
  20. MOLECULAR CONTROL OF CELL PROLIFERATION
    David Morgan; Fiscal Year: 2009
    ..These studies are also likely to illuminate general mechanisms of protein ubiquitination, a regulatory modification of importance throughout cell biology and human disease. ..
  21. Quantitative Analysis of RING E3 Ubiquitin Ligases
    Charles Brenner; Fiscal Year: 2007
    ..manner that does not involve a covalent intermediate, these molecules bind substrates and specific E2 ubiquitin conjugating enzymes to effect transfer of ubiquitin to Lys residues on substrates, on the E3 (autoubiquitination), and on ..
  22. Quantitative Analysis of RING E3 Ubiquitin Ligases
    Charles Brenner; Fiscal Year: 2007
    ..manner that does not involve a covalent intermediate, these molecules bind substrates and specific E2 ubiquitin conjugating enzymes to effect transfer of ubiquitin to Lys residues on substrates, on the E3 (autoubiquitination), and on ..
  23. UBIQUITIN-MEDIATED CIRCUITRY IN GENOMIC INTEGRITY
    Yong Wan; Fiscal Year: 2006
    ..Genetic and biochemical studies in yeast and mammals have demonstrated that several ubiquitin conjugating enzymes and ubiquitin protein ligases including Ube2A, Ube2B, Ube2rE, Ube2N, BRCA1/BARD1, CDH1/APC are ..