ubiquitin conjugating enzymes

Summary

Summary: A class of enzymes that form a thioester bond to UBIQUITIN with the assistance of UBIQUITIN-ACTIVATING ENZYMES. They transfer ubiquitin to the LYSINE of a substrate protein with the assistance of UBIQUITIN-PROTEIN LIGASES.

Top Publications

  1. ncbi Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain
    L Deng
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas 75390, USA
    Cell 103:351-61. 2000
  2. pmc Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination
    Kenji Watanabe
    Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan
    EMBO J 23:3886-96. 2004
  3. ncbi Structure of a c-Cbl-UbcH7 complex: RING domain function in ubiquitin-protein ligases
    N Zheng
    Cellular Biochemistry and Biophysics Program, Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cell 102:533-9. 2000
  4. pmc SUMO-targeted ubiquitin ligases in genome stability
    John Prudden
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 26:4089-101. 2007
  5. ncbi Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation
    Michael J Eddins
    Department of Biophysics and Biophysical Chemistry and the Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nat Struct Mol Biol 13:915-20. 2006
  6. ncbi TAK1 is a ubiquitin-dependent kinase of MKK and IKK
    C Wang
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9148, USA
    Nature 412:346-51. 2001
  7. pmc The error-free component of the RAD6/RAD18 DNA damage tolerance pathway of budding yeast employs sister-strand recombination
    Hengshan Zhang
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Proc Natl Acad Sci U S A 102:15954-9. 2005
  8. ncbi SUMO-modified PCNA recruits Srs2 to prevent recombination during S phase
    Boris Pfander
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nature 436:428-33. 2005
  9. ncbi Bcl10 activates the NF-kappaB pathway through ubiquitination of NEMO
    Honglin Zhou
    Department of Molecular Oncology, Genentech Inc 1 DNA Way, South San Francisco, California 94080, USA
    Nature 427:167-71. 2004
  10. pmc UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit
    Mathew J Garnett
    University of Cambridge, Department of Oncology and The Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge, CB2 OXZ, UK
    Nat Cell Biol 11:1363-9. 2009

Research Grants

Detail Information

Publications222 found, 100 shown here

  1. ncbi Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain
    L Deng
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas 75390, USA
    Cell 103:351-61. 2000
    ..These results unveil a new regulatory function for ubiquitin, in which IKK is activated through the assembly of K63-linked polyubiquitin chains...
  2. pmc Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination
    Kenji Watanabe
    Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan
    EMBO J 23:3886-96. 2004
    ..These results suggest that Rad18 is crucial for recruitment of poleta to the damaged site through protein-protein interaction and PCNA monoubiquitination...
  3. ncbi Structure of a c-Cbl-UbcH7 complex: RING domain function in ubiquitin-protein ligases
    N Zheng
    Cellular Biochemistry and Biophysics Program, Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cell 102:533-9. 2000
    ....
  4. pmc SUMO-targeted ubiquitin ligases in genome stability
    John Prudden
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 26:4089-101. 2007
    ..The DNA repair factor Rad60 and its human homolog NIP45, which contain SLDs, are candidate STUbL targets. Consistently, Rad60 and Slx8-Rfp mutants have similar DNA repair defects...
  5. ncbi Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation
    Michael J Eddins
    Department of Biophysics and Biophysical Chemistry and the Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nat Struct Mol Biol 13:915-20. 2006
    ..The structure reveals the key role of Mms2 in allowing selective insertion of Lys63 into the Ubc13 active site and suggests a molecular model for polyubiquitin chain elongation...
  6. ncbi TAK1 is a ubiquitin-dependent kinase of MKK and IKK
    C Wang
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9148, USA
    Nature 412:346-51. 2001
    ..We also provide evidence that TRAF6 is conjugated by the K63 polyubiquitin chains. These results indicate that ubiquitination has an important regulatory role in stress response pathways, including those of IKK and JNK...
  7. pmc The error-free component of the RAD6/RAD18 DNA damage tolerance pathway of budding yeast employs sister-strand recombination
    Hengshan Zhang
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Proc Natl Acad Sci U S A 102:15954-9. 2005
    ....
  8. ncbi SUMO-modified PCNA recruits Srs2 to prevent recombination during S phase
    Boris Pfander
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nature 436:428-33. 2005
    ..Our finding suggests a model in which SUMO-modified PCNA recruits Srs2 in S phase in order to prevent unwanted recombination events of replicating chromosomes...
  9. ncbi Bcl10 activates the NF-kappaB pathway through ubiquitination of NEMO
    Honglin Zhou
    Department of Molecular Oncology, Genentech Inc 1 DNA Way, South San Francisco, California 94080, USA
    Nature 427:167-71. 2004
    ..Thus, the adaptor protein Bcl10 promotes activation of NF-kappaB transcription factors through paracaspase- and UBC13-dependent ubiquitination of NEMO...
  10. pmc UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit
    Mathew J Garnett
    University of Cambridge, Department of Oncology and The Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge, CB2 OXZ, UK
    Nat Cell Biol 11:1363-9. 2009
    ..Thus, UBE2S functions with the APC/C in a two-step mechanism to control substrate ubiquitylation that is essential for mitotic exit after prolonged SAC activation, providing a new model for APC/C function in human cells...
  11. pmc Identification of a physiological E2 module for the human anaphase-promoting complex
    Adam Williamson
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 106:18213-8. 2009
    ..We conclude that UbcH10 and Ube2S constitute a physiological E2-module for APC/C, the activity of which is required for spindle assembly and cell division...
  12. ncbi A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase
    Radmila Janjusevic
    Laboratory of Structural Microbiology, Rockefeller University, New York, NY 10021, USA
    Science 311:222-6. 2006
    ..These results show that Pseudomonas syringae uses a mimic of host E3 ubiquitin ligases to inactivate plant defenses...
  13. ncbi Structure of a Shigella effector reveals a new class of ubiquitin ligases
    Yongqun Zhu
    National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China
    Nat Struct Mol Biol 15:1302-8. 2008
    ..Our analysis establishes a structurally and mechanistically distinct class of ubiquitin ligases found exclusively in pathogenic or symbiotic bacteria...
  14. ncbi Histone ubiquitination and chromatin remodeling in mouse spermatogenesis
    W M Baarends
    Department of Endocrinology and Reproduction, Erasmus University, Rotterdam, Rotterdam, 3000 DR, The Netherlands
    Dev Biol 207:322-33. 1999
    ..The postmeiotic uH2A immunoexpression in elongating spermatids indicates that nucleosome destabilization induced by histone ubiquitination may play a facilitating role during histone-to-protamine replacement...
  15. pmc DNA damage checkpoints are involved in postreplication repair
    Leslie Barbour
    Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
    Genetics 174:1789-800. 2006
    ..These results suggest that a damage checkpoint is essential for tolerance mediated by both the error-free and error-prone branches of PRR...
  16. pmc Mechanism of ubiquitin-chain formation by the human anaphase-promoting complex
    Lingyan Jin
    Department of Molecular and Cell Biology, 16 Barker Hall, University of California at Berkeley, Berkeley, CA 94720 3202, USA
    Cell 133:653-65. 2008
    ..We propose that recognition of similar motifs in substrates and ubiquitin enables the APC/C to assemble ubiquitin chains with the specificity and efficiency required for tight cell-cycle control...
  17. pmc APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex
    Z Tang
    Departments of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9041, USA
    Mol Biol Cell 12:3839-51. 2001
    ..Surprisingly, with Ubc4 as the E2 enzyme, Zn(2+) ions alone are sufficient to catalyze the ubiquitination of cyclin B1. Therefore, the Zn(2+) ions of the RING finger family of ubiquitin ligases may be directly involved in catalysis...
  18. pmc A ubiquitin mutant with specific defects in DNA repair and multiubiquitination
    J Spence
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115
    Mol Cell Biol 15:1265-73. 1995
    ..The results of this study suggest that Lys-63 is used as a linkage site in the formation of novel multiubiquitin chain structures that play an important role in DNA repair...
  19. pmc Quantitative proteomics reveals the function of unconventional ubiquitin chains in proteasomal degradation
    Ping Xu
    Department of Human Genetics, Center for Neurodegenerative Diseases, Emory University, Atlanta, GA 30322, USA
    Cell 137:133-45. 2009
    ..Ubc6 primarily synthesizes K11-linked chains, and K11 linkages function in the ERAD pathway. Thus, unconventional polyubiquitin chains are critical for ubiquitin-proteasome system function...
  20. ncbi Transient global cerebral ischemia induces a massive increase in protein sumoylation
    Wei Yang
    Department of Anesthesiology, Multidisciplinary Neuroprotection Laboratories, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Cereb Blood Flow Metab 28:269-79. 2008
    ....
  21. pmc DNA damage-dependent acetylation and ubiquitination of H2AX enhances chromatin dynamics
    Tsuyoshi Ikura
    Department of Biochemistry, Tohoku University Graduate School of Medicine, Seiryoumachi 2 1, Aobaku Sendai 980 8575, Japan
    Mol Cell Biol 27:7028-40. 2007
    ..We conclude that the sequential acetylation and ubiquitination of H2AX by TIP60-UBC13 promote enhanced histone dynamics, which in turn stimulate a DNA damage response...
  22. ncbi The SUMO conjugating enzyme Ubc9 is a regulator of GLUT4 turnover and targeting to the insulin-responsive storage compartment in 3T3-L1 adipocytes
    Li bin Liu
    Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Showa machi, Maebashi, Japan
    Diabetes 56:1977-85. 2007
    ..Thus, Ubc9 is a pivotal regulator of the insulin sensitivity of glucose transport in adipocytes...
  23. pmc Der3p/Hrd1p is required for endoplasmic reticulum-associated degradation of misfolded lumenal and integral membrane proteins
    J Bordallo
    Institut fur Biochemie, Universitat Stuttgart, Germany
    Mol Biol Cell 9:209-22. 1998
    ..Der3p might serve as a component programming the translocon for retrograde transport of ER proteins, or it might be involved in recognition through its lumenal RING-H2 motif of proteins of the ER that are destined for degradation...
  24. ncbi Identification of determinants in E2 ubiquitin-conjugating enzymes required for hect E3 ubiquitin-protein ligase interaction
    U Nuber
    Deutsches Krebsforschungszentrum, Angewandte Tumorvirologie, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany
    J Biol Chem 274:7576-82. 1999
    ..The conservation of this phenylalanine residue throughout evolution underlines the importance of the ability to interact with hect domain proteins for the cellular function of UBC4/UBC5 subfamily members...
  25. ncbi RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO
    Carsten Hoege
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany
    Nature 419:135-41. 2002
    ..We demonstrate that these modifications differentially affect resistance to DNA damage, and that damage-induced PCNA ubiquitination is elementary for DNA repair and occurs at the same conserved residue in yeast and humans...
  26. ncbi UbcH10 is the cancer-related E2 ubiquitin-conjugating enzyme
    Yoshiaki Okamoto
    Division of Biochemistry, Chiba Cancer Center Research Institute, Chiba 260 8717, Japan
    Cancer Res 63:4167-73. 2003
    ..Collectively, our present results provide the first evidence that UbcH10 is highly expressed in various human primary tumors and that UbcH10 has an ability to promote cell growth and malignant transformation...
  27. ncbi The nucleoporin RanBP2 has SUMO1 E3 ligase activity
    Andrea Pichler
    Max Planck Institute for Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany
    Cell 108:109-20. 2002
    ..Our findings place SUMOylation at the cytoplasmic filaments of the NPC and suggest that, at least for some substrates, modification and nuclear import are linked events...
  28. ncbi E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages
    Devin E Christensen
    Department of Biochemistry, University of Washington, Box 357350, Seattle, Washington 98195 7350, USA
    Nat Struct Mol Biol 14:941-8. 2007
    ..Thus, BRCA1 has the ability to direct the synthesis of specific polyubiquitin chain linkages, depending on the E2 bound to its RING...
  29. ncbi A proteolytic pathway that recognizes ubiquitin as a degradation signal
    E S Johnson
    Division of Biology, California Institute of Technology, Pasadena 91125, USA
    J Biol Chem 270:17442-56. 1995
    ....
  30. ncbi A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy through its PIASy-enhanced modification by SUMO-1 or SUMO-3
    Lalitha Subramanian
    Department of Pharmacology and Medical Scientist Training Program, University of Michigan Medical School, Ann Arbor, Michigan 48109 0632, USA
    J Biol Chem 278:9134-41. 2003
    ..Our results indicate that SUMO modification of SC motifs provides a means to rapidly control higher order interactions among transcription factors and suggests that SUMOylation may be a general mechanism to limit transcriptional synergy...
  31. doi The E2 ubiquitin-conjugating enzymes, AtUBC1 and AtUBC2, play redundant roles and are involved in activation of FLC expression and repression of flowering in Arabidopsis thaliana
    Lin Xu
    Institut de Biologie Moléculaire des plantes IBMP, Laboratoire Propre du CNRS UPR2357 conventionné avec l Université Louis Pasteur Strasbourg 1, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France
    Plant J 57:279-88. 2009
    ....
  32. ncbi A role for Ubc9 in tumorigenesis
    Yin Yuan Mo
    Department of Biopharmaceutical Sciences, University of Illinois at Chicago, Chicago, IL 60612, USA
    Oncogene 24:2677-83. 2005
    ..Together, these results suggest a role for Ubc9 in tumorigenesis at least partially through regulation of bcl-2 expression...
  33. ncbi Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation
    Bing Zhu
    Howard Hughes Medical Institute, Division of Nucleic Acids Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 683 Hoes Lane, Piscataway, New Jersey 08854, USA
    Mol Cell 20:601-11. 2005
    ..In contrast, RNAi against the RNF20/40 complex or hPAF complex reduces H2B monoubiquitination, lowers methylation levels at H3 lysines 4 and 79, and represses HOX gene expression...
  34. pmc Insights into E3 ligase activity revealed by a SUMO-RanGAP1-Ubc9-Nup358 complex
    David Reverter
    Structural Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
    Nature 435:687-92. 2005
    ....
  35. ncbi A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination
    Peter S Brzovic
    Department of Biochemistry, University of Washington, Seattle, 98195, USA
    Mol Cell 21:873-80. 2006
    ..Self-assembly has profound consequences for the processive formation of polyubiquitin (poly-Ub) chains in ubiquitination reactions directed by the breast and ovarian cancer tumor susceptibility protein BRCA1...
  36. pmc Covalent modification of the androgen receptor by small ubiquitin-like modifier 1 (SUMO-1)
    H Poukka
    Department of Physiology, Institute of Biomedicine, and Department of Clinical Chemistry, University of Helsinki, FIN 00014, Helsinki, Finland
    Proc Natl Acad Sci U S A 97:14145-50. 2000
    ..Taken together, our data suggest that reversible sumoylation is a mechanism for regulation of steroid receptor function...
  37. pmc Ubc9 interacts with a nuclear localization signal and mediates nuclear localization of the paired-like homeobox protein Vsx-1 independent of SUMO-1 modification
    A L Kurtzman
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794, USA
    Proc Natl Acad Sci U S A 98:5602-7. 2001
    ..These results suggest that Ubc9 mediates the nuclear localization of Vsx-1, and possibly other proteins, through a nonenzymatic mechanism that is independent of SUMO-1 conjugation...
  38. ncbi Targeting Ubc9 for cancer therapy
    Yin Yuan Mo
    Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University, PO Box 19626, Springfield, IL 62794, USA
    Expert Opin Ther Targets 9:1203-16. 2005
    ....
  39. doi SUMOylation regulates Rad18-mediated template switch
    Dana Branzei
    IFOM, the FIRC Institute for Molecular Oncology Foundation, IFOM IEO Campus, Via Adamello 16, 20139 Milan, Italy
    Nature 456:915-20. 2008
    ..Altogether, our results unmask a role for PCNA ubiquitylation and SUMOylation pathways in promoting transient damage-induced replication-coupled recombination events involving sister chromatids at replication forks...
  40. doi Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks
    Yuri Tsuji
    Cell Genetics, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan
    Genes Cells 13:343-54. 2008
    ..These results suggest that RAD18 complexed with RAD6B is recruited to stalled replication forks via interactions with forked DNA or long ssDNA structures, a process that is required for initiating PRR...
  41. ncbi Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme
    M A Kalchman
    Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4
    J Biol Chem 271:19385-94. 1996
    ..The huntingtin-E2-25K interaction is not obviously modulated by CAG length. We also demonstrate that huntingtin is ubiquitinated. These findings have implications for the regulated catabolism of the gene product for HD...
  42. ncbi Mechanisms underlying ubiquitination
    C M Pickart
    School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, Maryland 21205, USA
    Annu Rev Biochem 70:503-33. 2001
    ..The new findings shed light on many aspects of E3 structure, function, and mechanism, but also emphasize that key features of E3 catalysis remain to be elucidated...
  43. ncbi Ubc9p is the conjugating enzyme for the ubiquitin-like protein Smt3p
    E S Johnson
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021, USA
    J Biol Chem 272:26799-802. 1997
    ..cerevisiae. These results suggest that, like ubiquitination, Smt3p conjugation may be a critical modification in cell cycle regulation...
  44. ncbi Increased phosphorylation and dimethylation of XY body histones in the Hr6b-knockout mouse is associated with derepression of the X chromosome
    Willy M Baarends
    Department of Reproduction and Development, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    J Cell Sci 120:1841-51. 2007
    ..We conclude that HR6B exerts control over different histone modifications in spermatocytes and spermatids, and that this function contributes to the postmeiotic maintenance of X chromosome silencing...
  45. ncbi The Bur1/Bur2 complex is required for histone H2B monoubiquitination by Rad6/Bre1 and histone methylation by COMPASS
    Adam Wood
    Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Boulevard, St Louis, Missouri 63104, USA
    Mol Cell 20:589-99. 2005
    ..Our results identify in vivo substrates for Bur1/Bur2, thus linking its role to transcriptional elongation and demonstrating a potential activation mechanism for histone H2B monoubiquitination by the Rad6/Bre1 complex...
  46. ncbi ER degradation of a misfolded luminal protein by the cytosolic ubiquitin-proteasome pathway
    M M Hiller
    Institut für Biochemie der Universität Stuttgart, Pfaffenwaldring 55, D 70569 Stuttgart, Germany
    Science 273:1725-8. 1996
    ..It is likely that CPY* entered the ER, was glycosylated, and was then transported back out of the ER lumen to the cytoplasmic side of the organelle, where it was conjugated with ubiquitin and degraded...
  47. pmc Synthesis of free and proliferating cell nuclear antigen-bound polyubiquitin chains by the RING E3 ubiquitin ligase Rad5
    Candice M Carlile
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 284:29326-34. 2009
    ..These findings indicate that Rad5-mediated recognition of monoUb-PCNA in vivo is likely to depend upon interactions with additional factors at stalled replication forks...
  48. ncbi A novel ubiquitination factor, E4, is involved in multiubiquitin chain assembly
    M Koegl
    Zentrum fur Molekulare Biologie, Universitat Heidelberg, Germany
    Cell 96:635-44. 1999
    ..In yeast, E4 activity is linked to cell survival under stress conditions, indicating that eukaryotes utilize E4-dependent proteolysis pathways for multiple cellular functions...
  49. ncbi Ubiquitination of histone H2B regulates H3 methylation and gene silencing in yeast
    Zu Wen Sun
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908 0733, USA
    Nature 418:104-8. 2002
    ..We refer to this as 'trans-tail' regulation of histone modification, a stated prediction of the histone code hypothesis...
  50. ncbi Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9
    M H Tatham
    Institute of Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews KY16 5ST, United Kingdom
    J Biol Chem 276:35368-74. 2001
    ..The ability to form polymeric chains is not shared by SUMO-1, and although all SUMO species use the same conjugation machinery, modification by SUMO-1 and SUMO-2/-3 may have distinct functional consequences...
  51. pmc SUMO-1 modification activates the transcriptional response of p53
    M S Rodriguez
    School of Biomedical Science, BMS Building, University of St Andrews, St Andrews, Fife KY16 9ST
    EMBO J 18:6455-61. 1999
    ..The SUMO-1 modification pathway therefore acts as a potential regulator of the p53 response and may represent a novel target for the development of therapeutically useful modulators of the p53 response...
  52. ncbi Control of spontaneous and damage-induced mutagenesis by SUMO and ubiquitin conjugation
    Philipp Stelter
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
    Nature 425:188-91. 2003
    ..Our findings assign a function to SUMO during S phase and demonstrate how ubiquitin and SUMO, by regulating the accuracy of replication and repair, contribute to overall genomic stability...
  53. ncbi Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5
    U Nuber
    Deutsches Krabsforschungszentrum, Angewandte Tumorvirologie, Heidelberg, Federal Republic of Germany
    J Biol Chem 271:2795-800. 1996
    ..In addition, UbcH5 but not UbcH6 or UbcH7 efficiently interacts with the heet protein RSP5. These results indicate that E6-AP can interact with at least two species of E2 and that different hect proteins may interact with different E2s...
  54. ncbi Hrd1p/Der3p is a membrane-anchored ubiquitin ligase required for ER-associated degradation
    N W Bays
    Section of Cell and Developmental Biology, Division of Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, California 92093 0347, USA
    Nat Cell Biol 3:24-9. 2001
    ..In this capacity, Hrd1p has an apparent preference for misfolded proteins. We also show that Hrd1p functions as an E3 in vivo, using only Ubc7p or Ubc1p to specifically program the ubiquitination of ERAD substrates...
  55. ncbi Up-regulation of CIR1/CROC1 expression upon cell immortalization and in tumor-derived human cell lines
    L Ma
    Department of Pathology, McMaster University, Hamilton, Ontario, Canada
    Oncogene 17:1321-6. 1998
    ..These results are compatible with induction of CIR1/CROC1 being an early event in the acquisition of immortality and with a role for this gene in the immortal phenotype of tumor cells...
  56. ncbi Role of a ubiquitin-conjugating enzyme in degradation of S- and M-phase cyclins
    W Seufert
    Friedrich Miescher Laboratorium der Max Planck Gesellschaft, Tubingen, Germany
    Nature 373:78-81. 1995
    ..Thus distinct degradation signals or regulated interaction with the ubiquitin-protein ligase system may determine the cell-cycle specificity of cyclin proteolysis...
  57. ncbi The tyrosine kinase negative regulator c-Cbl as a RING-type, E2-dependent ubiquitin-protein ligase
    C A Joazeiro
    The Salk Institute, Molecular Biology and Virology Laboratory, La Jolla, CA 92037, USA
    Science 286:309-12. 1999
    ..These results reveal an SH2-containing protein that functions as a ubiquitin-protein ligase and thus provide a distinct mechanism for substrate targeting in the ubiquitin system...
  58. pmc Anatomy of the E2 ligase fold: implications for enzymology and evolution of ubiquitin/Ub-like protein conjugation
    A Maxwell Burroughs
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
    J Struct Biol 162:205-18. 2008
    ..These features are likely to form a critical mechanical element of the fold required for catalysis. The results presented here could aid in new experiments to understand E2 catalysis...
  59. pmc Different HECT domain ubiquitin ligases employ distinct mechanisms of polyubiquitin chain synthesis
    Min Wang
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    EMBO J 24:4324-33. 2005
    ..A small region near the N-terminus of the conserved HECT domain helps to bring about this functional distinction. Thus, a given HECT domain can specify both the linkage of a polyubiquitin chain and the mechanism of its assembly...
  60. ncbi A novel role for N-glycans in the ERAD system
    Yukiko Yoshida
    Laboratory of Frontier Science, Tokyo Metropolitan Institute of Medical Science, 3 18 22 Honkomagome, Bunkyo ku, Tokyo 113 8613
    J Biochem 134:183-90. 2003
    ..In this review, I describe recent advances in the molecular basis of the ERAD system, particularly those mediated by N-glycan recognition molecules...
  61. pmc Contributions of ubiquitin- and PCNA-binding domains to the activity of Polymerase eta in Saccharomyces cerevisiae
    Joanne L Parker
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, EN6 3LD, United Kingdom
    Nucleic Acids Res 35:881-9. 2007
    ..Like its mammalian homolog, budding yeast Polymerase eta itself is ubiquitylated in a manner dependent on its ubiquitin-binding domain...
  62. pmc Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens
    S Tateishi
    Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto 862 0976, Japan
    Proc Natl Acad Sci U S A 97:7927-32. 2000
    ..Stable transformants with hRad18 mutated in this motif become sensitive to UV, methyl methanesulfonate, and mitomycin C, and are defective in the replication of UV-damaged DNA. Thus, hRAD18 is a functional homologue of RAD18...
  63. ncbi A conserved cysteine is essential for Pex4p-dependent ubiquitination of the peroxisomal import receptor Pex5p
    Chris Williams
    Department of Medical Biochemistry, Academic Medical Center, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    J Biol Chem 282:22534-43. 2007
    ..Together, our results strongly suggest that Pex4p-dependent ubiquitination of Pex5p occurs on a cysteine residue...
  64. pmc RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells
    Jaehoon Kim
    The Rockefeller University, New York, NY 10065, USA
    Cell 137:459-71. 2009
    ..These studies establish the natural H2B ubiquitylation factors in human cells and also detail the mechanistic basis for H2B ubiquitylation and function during transcription...
  65. ncbi Structural and functional conservation of error-free DNA postreplication repair in Schizosaccharomyces pombe
    Morgan Brown
    Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada
    DNA Repair (Amst) 1:869-80. 2002
    ..Hence, our data strongly support the hypothesis that the PRR function mediated by UBC13-MMS2 is conserved throughout eukaryotes...
  66. ncbi Ubiquitin-dependent proteolytic control of SUMO conjugates
    Kristina Uzunova
    Institute for Genetics, University of Cologne, Zulpicher Strasse 47, Cologne, Germany
    J Biol Chem 282:34167-75. 2007
    ..Simultaneous inhibition of both mechanisms leads to severe phenotypic defects...
  67. ncbi Ubc9 is a novel modulator of the induction properties of glucocorticoid receptors
    Sunil Kaul
    Steroid Hormones Section, NIDDK Laboratory of Molecular and Cellular Biology, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 277:12541-9. 2002
    ..With high concentrations of Ubc9 and GR, Ubc9 binding to GR appears to be sufficient to permit Ubc9 to act independently of the GME...
  68. pmc Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks
    Akira Motegi
    Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 105:12411-6. 2008
    ..Our results suggest that HLTF and SHPRH are functional homologues of yeast Rad5 that cooperatively mediate PCNA polyubiquitination and maintain genomic stability...
  69. ncbi Ubiquitin-conjugating enzyme E2-25K increases aggregate formation and cell death in polyglutamine diseases
    Remko de Pril
    Graduate School Neurosciences Amsterdam, Netherlands Institute for Neuroscience, Meibergdreef 47, 1105 BA Amsterdam, The Netherlands
    Mol Cell Neurosci 34:10-9. 2007
    ..These results demonstrate that targeting by ubiquitination plays an important role in the pathology of polyglutamine diseases...
  70. ncbi The UBA domain: a sequence motif present in multiple enzyme classes of the ubiquitination pathway
    K Hofmann
    Swiss Institute for Experimental Cancer Research, Epalinges s Lausanne, Switzerland
    Trends Biochem Sci 21:172-3. 1996
  71. ncbi The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p
    Adam Wood
    Department of Biochemistry, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 278:34739-42. 2003
    ..Thus, in addition to its role during the elongation phase of transcription, the Paf1 complex appears to activate the function but not the placement of the Rad6-Bre1 ubiquitin-protein ligase at the promoters of active genes...
  72. pmc Structure of the anaphase-promoting complex/cyclosome interacting with a mitotic checkpoint complex
    Franz Herzog
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Science 323:1477-81. 2009
    ..These observations clarify the structural basis for the inhibition of APC/C by spindle checkpoint proteins...
  73. pmc Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen
    Ildiko Unk
    Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H 6726 Szeged, Hungary
    Proc Natl Acad Sci U S A 103:18107-12. 2006
    ....
  74. pmc UbcH10 overexpression may represent a marker of anaplastic thyroid carcinomas
    P Pallante
    Dipartimento di Biologia e Patologia Cellulare e Molecolare c o Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, Facoltà di Medicina e Chirurgia di Napoli, Universita degli Studi di Napoli Federico II, Italy
    Br J Cancer 93:464-71. 2005
    ..Taken together, these results would indicate that UbcH10 overexpression is involved in thyroid cell proliferation, and may represent a marker of thyroid anaplastic carcinomas...
  75. pmc Opposing effects of ubiquitin conjugation and SUMO modification of PCNA on replicational bypass of DNA lesions in Saccharomyces cerevisiae
    Lajos Haracska
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555 1061, USA
    Mol Cell Biol 24:4267-74. 2004
    ....
  76. ncbi SUMOylation regulates the transcriptional activity of JunB in T lymphocytes
    Johan Garaude
    Institut de Genetique Moleculaire de Montpellier, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 5535, 1919 Route de Mende, Montpellier Cedex 5, France
    J Immunol 180:5983-90. 2008
    ..Thus, our work demonstrates that sumoylation of JunB regulates its ability to induce cytokine gene transcription and likely plays a critical role in T cell activation...
  77. pmc Functional characterization of Rad18 domains for Rad6, ubiquitin, DNA binding and PCNA modification
    Valerie Notenboom
    Molecular Carcinogenesis and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nucleic Acids Res 35:5819-30. 2007
    ..Finally we find that the SAP domain, but not the zinc finger domain, is capable of DNA binding in vitro...
  78. ncbi Rpn4 is a physiological substrate of the Ubr2 ubiquitin ligase
    Li Wang
    Barbara Ann Karmanos Cancer Institute, Department of Pathology, Wayne State University School of Medicine, 110 Warren Avenue, Detroit, MI 48201, USA
    J Biol Chem 279:55218-23. 2004
    ..This study not only identified the ubiquitination apparatus for Rpn4 but also unveiled the first physiological substrate of Ubr2. The biological significance of Ubr2-mediated degradation of Rpn4 is also discussed...
  79. pmc Regulation of p53 localization and activity by Ubc13
    Aaron Laine
    Signal Transduction Program, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell Biol 26:8901-13. 2006
    ..Our findings point to a newly discerned mechanism important in the regulation of p53 organization, localization, and activity by Ubc13...
  80. pmc Mms2-Ubc13-dependent and -independent roles of Rad5 ubiquitin ligase in postreplication repair and translesion DNA synthesis in Saccharomyces cerevisiae
    Venkateswarlu Gangavarapu
    Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, Galveston, TX 77555 1061
    Mol Cell Biol 26:7783-90. 2006
    ..In contrast to the role of Rad5 in PRR, however, where its function is coupled with that of Mms2-Ubc13, Rad5 function in TLS would be largely independent of this ubiquitin-conjugating enzyme complex...
  81. ncbi An altered-specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair
    G Sebastiaan Winkler
    Department of Physiological Chemistry, University Medical Centre Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    J Mol Biol 337:157-65. 2004
    ..These are the first examples of altered-specificity E2-E3 enzyme pairs and give further insight into how E2-E3 specificity is obtained...
  82. ncbi UBE2V2 (MMS2) is not required for effective immunoglobulin gene conversion or DNA damage tolerance in DT40
    Laura J Simpson
    MRC Laboratory of Molecular Biology, Division of Protein and Nucleic Acid Chemistry, Hills Road, Cambridge, CB2 2QH, UK
    DNA Repair (Amst) 4:503-10. 2005
    ..Together these data suggest that signalling through PCNA ubiquitination is not required for immunoglobulin diversification in DT40...
  83. pmc TEB4 is a C4HC3 RING finger-containing ubiquitin ligase of the endoplasmic reticulum
    Gerco Hassink
    Department of Medical Microbiology, Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Biochem J 388:647-55. 2005
    ..These properties are reminiscent of E3 enzymes, which are involved in ER-associated protein degradation. TEB4 is an ER degradation substrate itself, promoting its own degradation in a RING finger- and proteasome-dependent manner...
  84. pmc Histone H2B ubiquitylation is associated with elongating RNA polymerase II
    Tiaojiang Xiao
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, 405 Mary Ellen Jones Bldg, Chapel Hill, NC 27599 7260, USA
    Mol Cell Biol 25:637-51. 2005
    ..Collectively, our results indicate a role for Rad6 and H2B ubiquitylation during the elongation cycle of transcription and suggest a mechanism by which H3 methylation may be regulated...
  85. ncbi Solution structure of the ubiquitin-conjugating enzyme UbcH5B
    Klaartje Houben
    Department of NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Mol Biol 344:513-26. 2004
    ..The possible implication of this conformational freedom of Asn77 for its catalytic function is discussed...
  86. ncbi The E2-C vihar is required for the correct spatiotemporal proteolysis of cyclin B and itself undergoes cyclical degradation
    Endre Mathe
    Cancer Research UK Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
    Curr Biol 14:1723-33. 2004
    ..Substrate specificity and temporal activity of the APC/C has been thought to lie primarily with its two activators, Cdc20/Fizzy and Cdh1/Fizzy-related...
  87. ncbi Regulation of Smurf2 ubiquitin ligase activity by anchoring the E2 to the HECT domain
    Abiodun A Ogunjimi
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Mol Cell 19:297-308. 2005
    ..Thus, E2 enzyme recognition by an E3 HECT enzyme is not constitutively competent and provides a point of control for regulating the ubiquitin ligase activity through the action of auxiliary proteins...
  88. ncbi Structure and interactions of the helical and U-box domains of CHIP, the C terminus of HSP70 interacting protein
    Zhen Xu
    Department of Molecular Cardiology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Biochemistry 45:4749-59. 2006
    ..Our results provide insights into conformational variability in the domain arrangement of CHIP and into U-box-mediated recruitment of UbcH5b for the ubiquitination of Hsp70 and Hsp90 substrates...
  89. pmc Arabidopsis COP10 forms a complex with DDB1 and DET1 in vivo and enhances the activity of ubiquitin conjugating enzymes
    Yuki Yanagawa
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8104, USA
    Genes Dev 18:2172-81. 2004
    ..Thus, the CDD complex may act as a ubiquitylation-promoting factor to regulate photomorphogenesis...
  90. ncbi Determinants of functionality in the ubiquitin conjugating enzyme family
    Peter J Winn
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Structure 12:1563-74. 2004
    ..The specificities of only the ScUbc4/Ubc5 and ScUbc1p orthologs are reflected in their L4 and L7 loops...
  91. ncbi Human disorders of ubiquitination and proteasomal degradation
    Yong hui Jiang
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Curr Opin Pediatr 16:419-26. 2004
    ..The relevant genes encode ubiquitin, ubiquitin enzymes (E1 and many E2s and E3s), deubiquitinating enzymes, proteasomal subunits, and substrates undergoing ubiquitination...
  92. ncbi Overexpression, genomic amplification and therapeutic potential of inhibiting the UbcH10 ubiquitin conjugase in human carcinomas of diverse anatomic origin
    Klaus W Wagner
    Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121, USA
    Oncogene 23:6621-9. 2004
    ..Together, these data demonstrate that UbcH10 plays an important role in tumor development and that its inhibition in combination with agonists of the TRAIL receptor may provide an enhanced therapeutic index...
  93. ncbi Protein-protein interactions within an E2-RING finger complex. Implications for ubiquitin-dependent DNA damage repair
    Helle D Ulrich
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
    J Biol Chem 278:7051-8. 2003
    ..Finally, this study demonstrates that beyond its cooperation with the UBC13-MMS2 dimer, RAD5 must have an additional role in DNA damage repair independent of its RING finger domain...
  94. ncbi Identification and characterization of UEV3, a human cDNA with similarities to inactive E2 ubiquitin-conjugating enzymes
    Matthias Kloor
    Department of Molecular Pathology, University of Heidelberg, Im Neuenheimer Feld 220 221, Heidelberg, Germany
    Biochim Biophys Acta 1579:219-24. 2002
    ....
  95. ncbi Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MAT alpha 2 repressor
    P Chen
    Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637
    Cell 74:357-69. 1993
    ..These data reveal an unexpected overlap in substrate specificity among diverse UBC enzymes and suggest a combinatorial mechanism of substrate selection in which UBC enzymes partition into multiple ubiquitination complexes...
  96. pmc The Saccharomyces cerevisiae RAD30 gene, a homologue of Escherichia coli dinB and umuC, is DNA damage inducible and functions in a novel error-free postreplication repair mechanism
    J P McDonald
    Section on DNA Replication, Repair and Mutagenesis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2725, USA
    Genetics 147:1557-68. 1997
    ..These findings suggest that RD30 participates in a novel error-free repair pathway dependent on RAD6 and RAD18, but independent of REV1, REV3, REV7 and RAD5...
  97. pmc Human Cdc34 and Rad6B ubiquitin-conjugating enzymes target repressors of cyclic AMP-induced transcription for proteolysis
    D Pati
    Texas Children s Cancer Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 19:5001-13. 1999
    ....
  98. ncbi Human ubiquitin-protein ligase Nedd4: expression, subcellular localization and selective interaction with ubiquitin-conjugating enzymes
    T Anan
    Department of Tumor Genetics and Biology, Kumamoto University School of Medicine, Japan
    Genes Cells 3:751-63. 1998
    ..Nedd4 has been reported to be involved in the selective ubiquitination of some regulatory proteins in transcription and membrane transport...
  99. ncbi Identification, expression, and chromosomal localization of ubiquitin conjugating enzyme 7 (UBE2G2), a human homologue of the Saccharomyces cerevisiae ubc7 gene
    N Katsanis
    Department of Neurogenetics, Imperial College School of Medicine at St Mary s, Norfolk Place, London, W2 1PG, United Kingdom
    Genomics 51:128-31. 1998
    ..is an essential step in the proteasome-dependent degradation process and is mediated by a family of ubiquitin conjugating enzymes (UBC). Several of these have been identified in a variety of organisms...
  100. pmc Role of UEV-1, an inactive variant of the E2 ubiquitin-conjugating enzymes, in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells
    E Sancho
    Departamento de Biologia Molecular, Instituto de Biología del Cáncer, IMIM CSIC, Barcelona, Spain
    Mol Cell Biol 18:576-89. 1998
    ..This was accompanied with a profound inhibition of the mitotic kinase, cdk1. These results suggest that UEV proteins are involved in the control of differentiation and could exert their effects by altering cell cycle distribution...
  101. pmc The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum
    S Fang
    Regulation of Protein Function Laboratory, Center for Cancer Research, National Cancer Institute, Building 10, Room 1B34, 9000 Rockville Pike, Bethesda, MD 20892 1152, USA
    Proc Natl Acad Sci U S A 98:14422-7. 2001
    ..gp78 has thus been found to be an example of a mammalian cellular E3 intrinsic to the ER, suggesting a potential link between ubiquitylation, ERAD, and metastasis...

Research Grants70

  1. MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSIS
    Per Olof Hasselgren; Fiscal Year: 2001
    ..e., inhibition of myofilament release by treatment with a calcium antagonist and inhibition of ubiquitin/proteasome-dependent degradation of the released myofilaments by a proteasome blocker. ..
  2. MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSIS
    Per Olof Hasselgren; Fiscal Year: 2002
    ..e., inhibition of myofilament release by treatment with a calcium antagonist and inhibition of ubiquitin/proteasome-dependent degradation of the released myofilaments by a proteasome blocker. ..
  3. MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSIS
    Per Olof Hasselgren; Fiscal Year: 2000
    ..e., inhibition of myofilament release by treatment with a calcium antagonist and inhibition of ubiquitin/proteasome-dependent degradation of the released myofilaments by a proteasome blocker. ..
  4. MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSIS
    Per Olof Hasselgren; Fiscal Year: 2001
    ..e., inhibition of myofilament release by treatment with a calcium antagonist and inhibition of ubiquitin/proteasome-dependent degradation of the released myofilaments by a proteasome blocker. ..
  5. MUSCLE PROTEIN TURNOVER AND AMINO ACID UPTAKE IN SEPSIS
    Per Olof Hasselgren; Fiscal Year: 2003
    ..e., inhibition of myofilament release by treatment with a calcium antagonist and inhibition of ubiquitin/proteasome-dependent degradation of the released myofilaments by a proteasome blocker. ..
  6. NUCLEAR HORMONE ACTION--ROLE OF UBIQUITIN PATHWAYS
    Zafar Nawaz; Fiscal Year: 2000
    ..Of the NEDD8 pathway as a co-activator of NHRs and to characterize this role in nuclear hormone receptor co-activation. ..
  7. Quantitative Analysis of RING E3 Ubiquitin Ligases
    Charles Brenner; Fiscal Year: 2009
    ..2003;Kirkpatrick et al., 2005a). Third, the E2 ubiquitin conjugating enzymes have different specificities and potentially form different sets of target protein and polyubiquitin ..
  8. NUCLEAR HORMONE ACTION--ROLE OF UBIQUITIN PATHWAYS
    Zafar Nawaz; Fiscal Year: 2001
    ..Of the NEDD8 pathway as a co-activator of NHRs and to characterize this role in nuclear hormone receptor co-activation. ..
  9. NUCLEAR HORMONE ACTION--ROLE OF UBIQUITIN PATHWAYS
    Zafar Nawaz; Fiscal Year: 2003
    ..Of the NEDD8 pathway as a co-activator of NHRs and to characterize this role in nuclear hormone receptor co-activation. ..
  10. NUCLEAR HORMONE ACTION--ROLE OF UBIQUITIN PATHWAYS
    Zafar Nawaz; Fiscal Year: 2002
    ..Of the NEDD8 pathway as a co-activator of NHRs and to characterize this role in nuclear hormone receptor co-activation. ..
  11. Quantitative Analysis of RING E3 Ubiquitin Ligases
    CHARLES M BRENNER; Fiscal Year: 2010
    ..2003;Kirkpatrick et al., 2005a). Third, the E2 ubiquitin conjugating enzymes have different specificities and potentially form different sets of target protein and polyubiquitin ..
  12. NUCLEAR HORMONE ACTION--ROLE OF UBIQUITIN PATHWAYS
    Zafar Nawaz; Fiscal Year: 2003
    ..Of the NEDD8 pathway as a co-activator of NHRs and to characterize this role in nuclear hormone receptor co-activation. ..
  13. NUCLEAR HORMONE ACTION--ROLE OF UBIQUITIN PATHWAYS
    Zafar Nawaz; Fiscal Year: 2002
    ..Of the NEDD8 pathway as a co-activator of NHRs and to characterize this role in nuclear hormone receptor co-activation. ..
  14. Structural Biology of U-box E3 Ubiquitin Ligases
    Walter J Chazin; Fiscal Year: 2012
    ..Despite the availability of structures of CHIP, E2 ubiquitin conjugating enzymes and heat shock proteins, the factors controlling the selection of ubiquitination sites on the ..
  15. Structural Biology of U-box E3 Ubiquitin Ligases
    Walter J Chazin; Fiscal Year: 2010
    ..Despite the availability of structures of CHIP, E2 ubiquitin conjugating enzymes and heat shock proteins, the factors controlling the selection of ubiquitination sites on the ..
  16. DISSECTION OF THE FUNCTIONS OF HERPES SIMPLEX VIRUS ICPO
    Bernard Roizman; Fiscal Year: 2004
    ..We expect that the proposed studies will help understand the role of ICP0 in the evolution of diseases caused by HSV-1 and HSV-2. ..
  17. DISSECTION OF THE FUNCTIONS OF HERPES SIMPLEX VIRUS ICPO
    Bernard Roizman; Fiscal Year: 2003
    ..We expect that the proposed studies will help understand the role of ICP0 in the evolution of diseases caused by HSV-1 and HSV-2. ..
  18. DISSECTION OF THE FUNCTIONS OF HERPES SIMPLEX VIRUS ICPO
    Bernard Roizman; Fiscal Year: 2005
    ..We expect that the proposed studies will help understand the role of ICP0 in the evolution of diseases caused by HSV-1 and HSV-2. ..
  19. DISSECTION OF THE FUNCTIONS OF HERPES SIMPLEX VIRUS ICPO
    Bernard Roizman; Fiscal Year: 2006
    ..We expect that the proposed studies will help understand the role of ICP0 in the evolution of diseases caused by HSV-1 and HSV-2. ..
  20. DISSECTION OF THE FUNCTIONS OF HERPES SIMPLEX VIRUS ICPO
    Bernard Roizman; Fiscal Year: 2007
    ..We expect that the proposed studies will help understand the role of ICP0 in the evolution of diseases caused by HSV-1 and HSV-2. ..
  21. EXPRESSION OF CELL CYCLE PROTEINS IN BREAST CANCER
    Michele Pagano; Fiscal Year: 1999
    ..in breast carcinomas, they will analyze tumor breast samples for the abundance of specific degradation activities and for the levels of various ubiquitin conjugating enzymes (Ubcs) involved in cell cycle regulation (Specific Aim 2).
  22. EXPRESSION OF CELL CYCLE PROTEINS IN BREAST CANCER
    Michele Pagano; Fiscal Year: 2000
    ..in breast carcinomas, they will analyze tumor breast samples for the abundance of specific degradation activities and for the levels of various ubiquitin conjugating enzymes (Ubcs) involved in cell cycle regulation (Specific Aim 2).
  23. Identifying Ubiquitin E3 Substrates with Redesigned E3s
    Brian Kuhlman; Fiscal Year: 2004
    ..Favorable results will indicate that this new method should be an excellent approach for identifying substrates of other E3 ubiquitin ligases important to health and disease. ..
  24. Identifying Ubiquitin E3 Substrates with Redesigned E3s
    Brian Kuhlman; Fiscal Year: 2005
    ..Favorable results will indicate that this new method should be an excellent approach for identifying substrates of other E3 ubiquitin ligases important to health and disease. ..
  25. FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
    Arthur Haas; Fiscal Year: 2003
    ..kDa polypeptides bear remarkable yet cryptic sequence similarity to the conserved catalytic domain of ubiquitin conjugating enzymes (Ubc). The present proposal comprises three specific aims...
  26. FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
    Arthur Haas; Fiscal Year: 2001
    ..kDa polypeptides bear remarkable yet cryptic sequence similarity to the conserved catalytic domain of ubiquitin conjugating enzymes (Ubc). The present proposal comprises three specific aims...
  27. FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
    Arthur Haas; Fiscal Year: 2002
    ..kDa polypeptides bear remarkable yet cryptic sequence similarity to the conserved catalytic domain of ubiquitin conjugating enzymes (Ubc). The present proposal comprises three specific aims...
  28. FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
    Arthur Haas; Fiscal Year: 2003
    ..kDa polypeptides bear remarkable yet cryptic sequence similarity to the conserved catalytic domain of ubiquitin conjugating enzymes (Ubc). The present proposal comprises three specific aims...
  29. FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
    Arthur Haas; Fiscal Year: 2000
    ..kDa polypeptides bear remarkable yet cryptic sequence similarity to the conserved catalytic domain of ubiquitin conjugating enzymes (Ubc). The present proposal comprises three specific aims...
  30. Structural and Functional Characterization of BRCA1/BARD1
    Rachel E Klevit; Fiscal Year: 2010
    ..A full description of the molecular interactions that are critical to BRCA1 function will provide new insight into the early events associated with loss of BRCA1 that lead to tumorogenesis. ..
  31. Analysis of ubiquitination enzymes in C. elegans
    Lynn Boyd; Fiscal Year: 2009
    ..Previous studies into the ubiquitin pathway in C. elegans identified more than 20 ubiquitin conjugating enzymes (Ubc) and greater than a hundred ubiquitin ligases (E3)...
  32. Structural and Functional Characterization of BRCA1/BARD1
    Rachel E Klevit; Fiscal Year: 2011
    ..A full description of the molecular interactions that are critical to BRCA1 function will provide new insight into the early events associated with loss of BRCA1 that lead to tumorogenesis. ..
  33. Structural and Functional Characterization of BRCA1/BARD1
    Rachel Klevit; Fiscal Year: 2009
    ..A full description of the molecular interactions that are critical to BRCA1 function will provide new insight into the early events associated with loss of BRCA1 that lead to tumorogenesis. ..
  34. Structural and Functional Characterization of BRCA1/BARD1
    Rachel Klevit; Fiscal Year: 2009
    ..A full description of the molecular interactions that are critical to BRCA1 function will provide new insight into the early events associated with loss of BRCA1 that lead to tumorogenesis. ..
  35. Functional Anatomy of the Cul3 Ubiquitin Ligase
    Jeffrey Harper; Fiscal Year: 2007
    The SCF ubiquitin ligase pathway employs cullin proteins to assemble substrate specificity modules and ubiquitin conjugating enzymes, thereby promoting substrate ubiquitination...
  36. Functional Anatomy of the Cul3 Ubiquitin Ligase
    Jeffrey Harper; Fiscal Year: 2006
    The SCF ubiquitin ligase pathway employs cullin proteins to assemble substrate specificity modules and ubiquitin conjugating enzymes, thereby promoting substrate ubiquitination...
  37. Functional Anatomy of the Cul3 Ubiquitin Ligase
    Jeffrey Harper; Fiscal Year: 2005
    The SCF ubiquitin ligase pathway employs cullin proteins to assemble substrate specificity modules and ubiquitin conjugating enzymes, thereby promoting substrate ubiquitination...
  38. ROLES OF UBIQUITIN CONJUGATING ENZYMES IN DNA REPAIR
    Zhiyuan Shen; Fiscal Year: 2001
    ....
  39. REGULATION OF GROWTH ARREST IN HYPEROXIC NEONATAL LUNG
    Sharon McGrath Morrow; Fiscal Year: 2001
    ..growth arrest secondary to hyperoxia in the neonatal lung is associated with the down regulation of ubiquitin conjugating enzymes necessary for the degradation of specific cyclin inhibitors...
  40. ROLES OF UBIQUITIN CONJUGATING ENZYMES IN DNA REPAIR
    Zhiyuan Shen; Fiscal Year: 2000
    ....
  41. REGULATION OF GROWTH ARREST IN HYPEROXIC NEONATAL LUNG
    Sharon McGrath Morrow; Fiscal Year: 2000
    ..growth arrest secondary to hyperoxia in the neonatal lung is associated with the down regulation of ubiquitin conjugating enzymes necessary for the degradation of specific cyclin inhibitors...
  42. FASEB CONFERENCE ON UBIQUITIN AND PROTEIN DEGRADATION
    George Demartino; Fiscal Year: 2001
    ..The small size of the conference, and its location and format are designed to foster maximal interactions among the 175 participants. ..
  43. ROLES OF UBIQUITIN CONJUGATING ENZYMES IN DNA REPAIR
    Zhiyuan Shen; Fiscal Year: 1999
    ....
  44. ROLES OF UBIQUITIN CONJUGATING ENZYMES IN DNA REPAIR
    Zhiyuan Shen; Fiscal Year: 2000
    ....
  45. REGULATION OF GROWTH ARREST IN HYPEROXIC NEONATAL LUNG
    Sharon McGrath Morrow; Fiscal Year: 1999
    ..growth arrest secondary to hyperoxia in the neonatal lung is associated with the down regulation of ubiquitin conjugating enzymes necessary for the degradation of specific cyclin inhibitors...
  46. NOVEL DEUBIQUITINATING ENZYME, UBP46, IN HEMATOPOIESIS
    Dong Er Zhang; Fiscal Year: 2000
    ..Two major groups of enzymes, ubiquitin conjugating enzymes and deubiquitinating enzymes, regulate the balance of protein ubiquitination...
  47. NOVEL DEUBIQUITINATING ENZYME, UBP46, IN HEMATOPOIESIS
    Dong Er Zhang; Fiscal Year: 1999
    ..Two major groups of enzymes, ubiquitin conjugating enzymes and deubiquitinating enzymes, regulate the balance of protein ubiquitination...
  48. NOVEL DEUBIQUITINATING ENZYME, UBP46, IN HEMATOPOIESIS
    Dong Er Zhang; Fiscal Year: 2001
    ..Two major groups of enzymes, ubiquitin conjugating enzymes and deubiquitinating enzymes, regulate the balance of protein ubiquitination...
  49. Analysis of ubiquitination enzymes in C. elegans
    Lynn Boyd; Fiscal Year: 2005
    ..typically achieved via a three-step pathway utilizing the E1 ubiquitin activating enzyme (E1), the E2 ubiquitin conjugating enzymes (Ubc), and the E3 ubiquitin ligases (E3)...
  50. NOVEL DEUBIQUITINATING ENZYME, UBP46, IN HEMATOPOIESIS
    Dong Er Zhang; Fiscal Year: 2002
    ..Two major groups of enzymes, ubiquitin conjugating enzymes and deubiquitinating enzymes, regulate the balance of protein ubiquitination...
  51. Immune evasion by gamma 2 Herpesviruses
    Klaus Frueh; Fiscal Year: 2009
    ..abstract_text> ..
  52. Immune evasion by gamma 2 Herpesviruses
    KLAUS J FRUEH; Fiscal Year: 2010
    ..abstract_text> ..
  53. Hematopoiesis by Ubiquitination and Deubiquitination
    ALAN D ANDREA; Fiscal Year: 2005
    ..Protein ubiquitination is controlled by the coordinate action of ubiquitin conjugating enzymes and deubiquitinating enzymes...
  54. Hematopoiesis by Ubiquitination and Deubiquitination
    ALAN D ANDREA; Fiscal Year: 2002
    ..Protein ubiquitination is controlled by the coordinate action of ubiquitin conjugating enzymes and deubiquitinating enzymes...
  55. Hematopoiesis by Ubiquitination and Deubiquitination
    ALAN D ANDREA; Fiscal Year: 2004
    ..Protein ubiquitination is controlled by the coordinate action of ubiquitin conjugating enzymes and deubiquitinating enzymes...
  56. Hematopoiesis by Ubiquitination and Deubiquitination
    ALAN D ANDREA; Fiscal Year: 2003
    ..Protein ubiquitination is controlled by the coordinate action of ubiquitin conjugating enzymes and deubiquitinating enzymes...
  57. ATP-UBIQUITIN-DEPENDENT PROTEOLYSIS
    Arthur Haas; Fiscal Year: 2006
    ..Overall, the proposed studies seek to apply conventional biochemical and genetic methods to the detailed examination of key processes within ubiquitin-dependent 26S proteasome targeting at the molecular level. ..
  58. ATP-UBIQUITIN-DEPENDENT PROTEOLYSIS
    Arthur Haas; Fiscal Year: 2003
    ..Overall, the proposed studies seek to apply conventional biochemical and genetic methods to the detailed examination of key processes within ubiquitin-dependent 26S proteasome targeting at the molecular level. ..
  59. ATP-UBIQUITIN-DEPENDENT PROTEOLYSIS
    Arthur Haas; Fiscal Year: 2006
    ..Overall, the proposed studies seek to apply conventional biochemical and genetic methods to the detailed examination of key processes within ubiquitin-dependent 26S proteasome targeting at the molecular level. ..
  60. MOLECULAR CONTROL OF CELL PROLIFERATION
    DAVID OWEN MORGAN; Fiscal Year: 2010
    ..These studies are also likely to illuminate general mechanisms of protein ubiquitination, a regulatory modification of importance throughout cell biology and human disease. ..
  61. MOLECULAR CONTROL OF CELL PROLIFERATION
    David Morgan; Fiscal Year: 2009
    ..These studies are also likely to illuminate general mechanisms of protein ubiquitination, a regulatory modification of importance throughout cell biology and human disease. ..
  62. MOLECULAR CONTROL OF CELL PROLIFERATION
    David Morgan; Fiscal Year: 2009
    ..These studies are also likely to illuminate general mechanisms of protein ubiquitination, a regulatory modification of importance throughout cell biology and human disease. ..
  63. MOLECULAR CONTROL OF CELL PROLIFERATION
    David Morgan; Fiscal Year: 2007
    ..These studies are also likely to illuminate general mechanisms of protein ubiquitination, a regulatory modification of importance throughout cell biology and human disease. ..
  64. Quantitative Analysis of RING E3 Ubiquitin Ligases
    Charles Brenner; Fiscal Year: 2007
    ..manner that does not involve a covalent intermediate, these molecules bind substrates and specific E2 ubiquitin conjugating enzymes to effect transfer of ubiquitin to Lys residues on substrates, on the E3 (autoubiquitination), and on ..
  65. Quantitative Analysis of RING E3 Ubiquitin Ligases
    Charles Brenner; Fiscal Year: 2007
    ..manner that does not involve a covalent intermediate, these molecules bind substrates and specific E2 ubiquitin conjugating enzymes to effect transfer of ubiquitin to Lys residues on substrates, on the E3 (autoubiquitination), and on ..
  66. Analysis of CHIP, a ubiquitin ligase implicated in neurodegeneration
    KENNETH MATTHEW SCAGLIONE; Fiscal Year: 2010
    ..regulate the types of ubiquitin chain linkages formed by CHIP;tested candidates will include all known ubiquitin conjugating enzymes (E2s) as well as a panel of selected de ubiquitinating enzymes (DUBs) and Ubiquitin Interacting Motif-..
  67. Analysis of CHIP, a ubiquitin ligase implicated in neurodegeneration
    KENNETH SCAGLIONE; Fiscal Year: 2009
    ..regulate the types of ubiquitin chain linkages formed by CHIP;tested candidates will include all known ubiquitin conjugating enzymes (E2s) as well as a panel of selected de ubiquitinating enzymes (DUBs) and Ubiquitin Interacting Motif-..
  68. RETICULOCYTE-SPECIFIC UBIQUITINATING ENZYMES
    Cecile Pickart; Fiscal Year: 1992
    ..In addition, the experiments are expected to yield major insights into the mechanism of erythroid differentiation, with possible applications in the understanding of congenital diserythropoietic anemias...
  69. UBIQUITIN-MEDIATED CIRCUITRY IN GENOMIC INTEGRITY
    Yong Wan; Fiscal Year: 2006
    ..Genetic and biochemical studies in yeast and mammals have demonstrated that several ubiquitin conjugating enzymes and ubiquitin protein ligases including Ube2A, Ube2B, Ube2rE, Ube2N, BRCA1/BARD1, CDH1/APC are ..
  70. UBIQUITIN-MEDIATED CIRCUITRY IN GENOMIC INTEGRITY
    Yong Wan; Fiscal Year: 2005
    ..Genetic and biochemical studies in yeast and mammals have demonstrated that several ubiquitin conjugating enzymes and ubiquitin protein ligases including Ube2A, Ube2B, Ube2rE, Ube2N, BRCA1/BARD1, CDH1/APC are ..