aldose ketose isomerases

Summary

Summary: Enzymes that catalyze the interconversion of aldoses and ketoses. EC 5.3.1.

Top Publications

  1. ncbi The 1.9 A resolution structure of Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a potential drug target
    Lena M Henriksson
    Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, SE 751 24 Uppsala, Sweden
    Acta Crystallogr D Biol Crystallogr 62:807-13. 2006
  2. ncbi Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugs
    H Jomaa
    Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, Friedrichstrasse 24, D 35392 Giessen, Germany
    Science 285:1573-6. 1999
  3. pmc A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis
    S Takahashi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 95:9879-84. 1998
  4. ncbi Targeting the methyl erythritol phosphate (MEP) pathway for novel antimalarial, antibacterial and herbicidal drug discovery: inhibition of 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) enzyme
    Nidhi Singh
    Department of Medicinal Chemistry and Laboratory for Applied Drug Design and Synthesis, School of Pharmacy, University of Mississippi, University, MS 38677 1848, USA
    Curr Pharm Des 13:1161-77. 2007
  5. ncbi Crystal structure of D-ribose-5-phosphate isomerase (RpiA) from Escherichia coli
    Erumbi S Rangarajan
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    Proteins 48:737-40. 2002
  6. pmc 1-Deoxy-D-xylulose 5-phosphate reductoisomerase (IspC) from Mycobacterium tuberculosis: towards understanding mycobacterial resistance to fosmidomycin
    Rakesh K Dhiman
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Bacteriol 187:8395-402. 2005
  7. pmc Dissolution of xylose metabolism in Lactococcus lactis
    K A Erlandson
    Department of Food Science, Cornell University, Ithaca, New York 14853, USA
    Appl Environ Microbiol 66:3974-80. 2000
  8. ncbi The crystal structure of E.coli 1-deoxy-D-xylulose-5-phosphate reductoisomerase in a ternary complex with the antimalarial compound fosmidomycin and NADPH reveals a tight-binding closed enzyme conformation
    Aengus Mac Sweeney
    Morphochem AG, WRO 1055 338, Schwarzwaldallee 215, CH 4058, Basel, Switzerland
    J Mol Biol 345:115-27. 2005
  9. ncbi Evaluation of fosmidomycin analogs as inhibitors of the Synechocystis sp. PCC6803 1-deoxy-D-xylulose 5-phosphate reductoisomerase
    Youn Hi Woo
    Department of Pharmaceutical Sciences, College of Pharmacy, Pharmacy Bldg Rm 203, Oregon State University, Corvallis, OR 97331 3507, USA
    Bioorg Med Chem 14:2375-85. 2006
  10. ncbi Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase
    C Vieille
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA
    Eur J Biochem 268:6291-301. 2001

Research Grants

Detail Information

Publications185 found, 100 shown here

  1. ncbi The 1.9 A resolution structure of Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a potential drug target
    Lena M Henriksson
    Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, SE 751 24 Uppsala, Sweden
    Acta Crystallogr D Biol Crystallogr 62:807-13. 2006
    ..Furthermore, the new structure represents an intermediate conformation between the open apo form and the closed holo form observed previously, giving insights into the conformational changes associated with catalysis...
  2. ncbi Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugs
    H Jomaa
    Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, Friedrichstrasse 24, D 35392 Giessen, Germany
    Science 285:1573-6. 1999
    ..Both drugs suppressed the in vitro growth of multidrug-resistant P. falciparum strains. After therapy with these drugs, mice infected with the rodent malaria parasite P. vinckei were cured...
  3. pmc A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis
    S Takahashi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 95:9879-84. 1998
    ..coli...
  4. ncbi Targeting the methyl erythritol phosphate (MEP) pathway for novel antimalarial, antibacterial and herbicidal drug discovery: inhibition of 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) enzyme
    Nidhi Singh
    Department of Medicinal Chemistry and Laboratory for Applied Drug Design and Synthesis, School of Pharmacy, University of Mississippi, University, MS 38677 1848, USA
    Curr Pharm Des 13:1161-77. 2007
    ....
  5. ncbi Crystal structure of D-ribose-5-phosphate isomerase (RpiA) from Escherichia coli
    Erumbi S Rangarajan
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    Proteins 48:737-40. 2002
  6. pmc 1-Deoxy-D-xylulose 5-phosphate reductoisomerase (IspC) from Mycobacterium tuberculosis: towards understanding mycobacterial resistance to fosmidomycin
    Rakesh K Dhiman
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Bacteriol 187:8395-402. 2005
    ..Thus, M. tuberculosis resistance to fosmidomycin is not due to intrinsic properties of Rv2870c, and the enzyme appears to be a valid drug target in this pathogen...
  7. pmc Dissolution of xylose metabolism in Lactococcus lactis
    K A Erlandson
    Department of Food Science, Cornell University, Ithaca, New York 14853, USA
    Appl Environ Microbiol 66:3974-80. 2000
    ..Nevertheless, either cumulatively or because of indirect affects on the structures of catalytic sites, these mutations render some strains of L. lactis unable to metabolize xylose...
  8. ncbi The crystal structure of E.coli 1-deoxy-D-xylulose-5-phosphate reductoisomerase in a ternary complex with the antimalarial compound fosmidomycin and NADPH reveals a tight-binding closed enzyme conformation
    Aengus Mac Sweeney
    Morphochem AG, WRO 1055 338, Schwarzwaldallee 215, CH 4058, Basel, Switzerland
    J Mol Biol 345:115-27. 2005
    ..The structure of the substrate complex must be interpreted with caution due to the presence of a second diastereomer in the active site...
  9. ncbi Evaluation of fosmidomycin analogs as inhibitors of the Synechocystis sp. PCC6803 1-deoxy-D-xylulose 5-phosphate reductoisomerase
    Youn Hi Woo
    Department of Pharmaceutical Sciences, College of Pharmacy, Pharmacy Bldg Rm 203, Oregon State University, Corvallis, OR 97331 3507, USA
    Bioorg Med Chem 14:2375-85. 2006
    ..PCC6803 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR). Fosfoxacin, the phosphate analog of fosmidomycin, and its acetyl congener were found to be more potent inhibitors of DXR than fosmidomycin...
  10. ncbi Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase
    C Vieille
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA
    Eur J Biochem 268:6291-301. 2001
    ..Thus, it would appear that protein thermostability is a function of more complex molecular determinants than amino-acid content alone...
  11. ncbi Crystal structure of yeast Ypr118w, a methylthioribose-1-phosphate isomerase related to regulatory eIF2B subunits
    Mario Bumann
    Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, Berne CH 3012
    J Biol Chem 279:37087-94. 2004
    ....
  12. ncbi Novel deoxyxylulosephosphate-reductoisomerase inhibitors: fosmidomycin derivatives with spacious acyl residues
    Regina Ortmann
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marburg, Germany
    Arch Pharm (Weinheim) 340:483-90. 2007
    ....
  13. pmc Restoration of a defective Lactococcus lactis xylose isomerase
    Joo Heon Park
    Department of Food Science, Cornell University, Ithaca, NY 14853, USA
    Appl Environ Microbiol 70:4318-25. 2004
    ..The dissolution of XI activity in L. lactis subsp. lactis involves a series of mutations that collectively eliminate enzyme activity by reducing the solubility of the enzyme...
  14. ncbi Crystallographic structures of two bisphosphonate:1-deoxyxylulose-5-phosphate reductoisomerase complexes
    Shunsuke Yajima
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    J Am Chem Soc 126:10824-5. 2004
    ..The availability of these two new crystal structures opens up the possibility of the further development of bisphosphonates and related systems as DXR inhibitors and, potentially, as antiinfective agents...
  15. ncbi Regulation of expression, genetic organization and substrate specificity of xylose uptake in Bacillus megaterium
    D Schmiedel
    Lehrstuhl fur Mikrobiologie, Institut fur Mikrobiologie, Biochemie und Genetik, Friedrich Alexander Universitat Erlangen Nurnberg, Germany
    Mol Microbiol 23:1053-62. 1997
    ..XylT has an apparent Michaelis constant (KM) of approx. 100 microM and is competitively inhibited by glucose with an inhibitor constant KI of 16 mM...
  16. ncbi Xylose isomerase from Escherichia coli. Characterization of the protein and the structural gene
    G D Schellenberg
    J Biol Chem 259:6826-32. 1984
    ..These results establish that the NH2-terminal methionine of xylose isomerase is specified by an ATG which is 7 nucleotides downstream from a Shine-Dalgarno sequence...
  17. ncbi Structure and mechanism of L-fucose isomerase from Escherichia coli
    J E Seemann
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Freiburg im Breisgau, Germany
    J Mol Biol 273:256-68. 1997
    ..Most likely, L-fucitol mimics a bound L-fucose molecule in its open chain form. The protein environment suggests strongly that the reaction belongs to the ene-diol type...
  18. ncbi Synthesis and evaluation of 1-deoxy-D-xylulose 5-phosphate analogues as chelation-based inhibitors of methylerythritol phosphate synthase
    Joel R Walker
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    J Org Chem 70:9955-9. 2005
    ..The carboxylate (1), methyl ester (3), amide (4), and alcohol (5) analogues were inhibitors with IC50's ranging from 0.25 to 1.0 mM. The hydroxamic acid (2) and amino (6) analogues did not inhibit the enzyme...
  19. ncbi Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus licheniformis encoded regulon for xylose utilization
    A Scheler
    Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Erlangen, Federal Republic of Germany
    Arch Microbiol 155:526-34. 1991
    ..The next greater differences are found to the S. xylosus and the greatest to the E. coli encoded genes. These results are discussed with respect to the taxonomic relations of these bacteria...
  20. pmc The 2.2 A resolution structure of RpiB/AlsB from Escherichia coli illustrates a new approach to the ribose-5-phosphate isomerase reaction
    Rong guang Zhang
    Biosciences Division, Structural Biology Center, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, USA
    J Mol Biol 332:1083-94. 2003
    ..The sequence and structural results further suggest that the two homologous components of LacAB (galactose-6-phosphate isomerase) will compose a bi-functional enzyme; the second activity is unknown...
  21. ncbi Characterization of native and histidine-tagged deoxyxylulose 5-phosphate reductoisomerase from the cyanobacterium Synechocystis sp. PCC6803
    Xihou Yin
    College of Pharmacy, Pharmacy Building, Room 203, Oregon State University, Corvallis, OR 97331 3507, USA
    Biochim Biophys Acta 1652:75-81. 2003
    ..The K(m)'s for the metal ions Mn(2+), Mg(2+), and Co(2+) were determined for native DXR for the first time, with the K(m) for Mg(2+) being approximately 200-fold higher than the K(m)'s for Mn(2+) and Co(2+)...
  22. pmc Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: implications for catalytic mechanism
    Haruka Tamura
    Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Osaka, Japan
    Protein Sci 17:126-35. 2008
    ..The highly conserved residues at the active site, namely, Cys160 and Asp240 are most likely to be involved in catalysis. The structural analysis sheds light on its catalytic mechanism of M1Pi...
  23. ncbi Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase complexed with cofactors: implications of a flexible loop movement upon substrate binding
    Shunsuke Yajima
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    J Biochem 131:313-7. 2002
    ..A flexible loop covering the substrate binding site plays an important role in the enzymatic reaction and the determination of substrate specificity...
  24. ncbi Synthesis and biological evaluation of cyclopropyl analogues of fosmidomycin as potent Plasmodium falciparum growth inhibitors
    Vincent Devreux
    Laboratory for Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B 9000 Gent, Belgium
    J Med Chem 49:2656-60. 2006
    ..coli DOXP reductoisomerase and proved equally active when tested in vitro for P. falciparum growth inhibition. Conversely, the alpha-phenyl cis-cyclopropyl analogue 4 showed virtually no inhibition of the enzyme...
  25. ncbi Identification of class 2 1-deoxy-D-xylulose 5-phosphate synthase and 1-deoxy-D-xylulose 5-phosphate reductoisomerase genes from Ginkgo biloba and their transcription in embryo culture with respect to ginkgolide biosynthesis
    Sang Min Kim
    School of Agricultural Biotechnology, Seoul National University, Korea
    Planta Med 72:234-40. 2006
    ..Exclusive transcription of ginkgolide biosynthesis-specific LPS and GbDXS2 in roots and the appearance of ginkgolides in leaves was consistent with translocation of the compounds from roots to leaves...
  26. ncbi Crystal structure of 1-deoxy-D-xylulose-5-phosphate reductoisomerase, a crucial enzyme in the non-mevalonate pathway of isoprenoid biosynthesis
    Klaus Reuter
    Institut fur Pharmazeutische Chemie, Philipps Universitat, Marbacher Weg 6, D 35032 Marburg, Germany
    J Biol Chem 277:5378-84. 2002
    ..A possible involvement of this loop in an induced fit during substrate binding is discussed...
  27. ncbi Inhibition of isoprene biosynthesis pathway enzymes by phosphonates, bisphosphonates, and diphosphates
    Feng Cheng
    Departments of Chemistry and Biophysics, 600 South Mathews Avenue, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    J Med Chem 47:5149-58. 2004
    ....
  28. ncbi 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening
    M Rodriguez-Concepcion
    Departament de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, Martí i Franquès 1 7, 08028 Barcelona, Spain
    Plant J 27:213-22. 2001
    ..Furthermore, the complete arrest of tomato seedling development using fosmidomycin confirms a key role of the MEP pathway in plant development...
  29. ncbi On the multiple functional roles of the active site histidine in catalysis and allosteric regulation of Escherichia coli glucosamine 6-phosphate deaminase
    G M Montero-Morán
    Departamento de Bioquimica, Laboratorio de Fisicoquímica y Diseño de Proteínas, Facultad de Medicina, Universidad Nacional Autonoma de Mexico UNAM, P O Box 70 159, Mexico City 04510, D F, Mexico
    Biochemistry 40:10187-96. 2001
    ..This, in turn, indicates that the interaction Glu148-His143 in the wild-type enzyme in the R state contributes to make the enzyme functional over a wide pH range...
  30. ncbi Antisense and chemical suppression of the nonmevalonate pathway affects ent-kaurene biosynthesis in Arabidopsis
    Kazunori Okada
    Department of Biology, Tokyo Gakugei University, Nukuikitamachi 4 1 1, Koganei Shi, Tokyo, 184 8501, Japan
    Planta 215:339-44. 2002
    ..These observations suggest that both AtMECT and DXR are important in the synthesis of isopentenyl diphosphate and dimethylallyl diphosphate and that ent-kaurene is mainly produced through the nonmevalonate pathway in the plastid...
  31. ncbi Molecular cloning and characterization of a 1-deoxy-D-xylulose 5-phosphate reductoisomerase gene from Ginkgo biloba
    Yifu Gong
    Plant Biotechnology Research Center, School of Agriculture and Biology, School of Life Science and Technology, Shanghai Jiao Tong University, Shanghai 200030, People s Republic of China
    DNA Seq 16:111-20. 2005
    ..biloba than that of other tissues. The cloning and characterization of GbDXR will be helpful to understand more about the role of DXR involved in the ginkgolides biosynthesis at the molecular level...
  32. ncbi Structure of 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase from Shewanella oneidensis at 1.6 A: identification of farnesyl pyrophosphate trapped in a hydrophobic cavity
    Shuisong Ni
    Pacific Northwest National Laboratory, Biological Sciences Division, Richland, WA 99352, USA
    Acta Crystallogr D Biol Crystallogr 60:1949-57. 2004
    ....
  33. ncbi Kinetic and chemical mechanism of Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate isomeroreductase
    Argyrides Argyrou
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 43:4375-84. 2004
    ..The results are discussed in terms of significant differences in the commitment factors for the various metal ions and pyridine nucleotides...
  34. ncbi Crystal structure of 1-deoxy-d-xylulose-5-phosphate reductoisomerase from Zymomonas mobilis at 1.9-A resolution
    Stefano Ricagno
    Department of Medical Biochemistry and Biophysics, Division of Molecular Structural Biology, Karolinska Institutet, Scheelevagen 2, S 171 77 Stockholm, Sweden
    Biochim Biophys Acta 1698:37-44. 2004
    ..However, there are differences in the recognition of the adenine ring of NADPH in the two enzymes...
  35. ncbi Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study
    Rodrigo Arreola
    Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, PO Box 510 3, Cuernavaca, 62250 Morelos, Mexico
    FEBS Lett 551:63-70. 2003
    ..These structural differences can be related to the kinetic and allosteric properties of both mammalian enzymes...
  36. ncbi Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily?
    Florence Vincent
    Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, UK
    J Biol Chem 280:19649-55. 2005
    ..The structure completes the NagB superfamily structural landscape and thus allows further interrogation of genomic data in terms of the regulation of NagB and the metabolic fate(s) of glucosamine 6-phosphate...
  37. ncbi High-throughput screen for inhibitors of 1-deoxy-d-xylulose 5-phosphate reductoisomerase by surrogate ligand competition
    Elizabeth B Gottlin
    Karo Bio USA, Inc, Durham, NC, USA
    J Biomol Screen 8:332-9. 2003
    ..The results presented here suggest that peptide surrogate ligands may be useful in formatting HTS for proteins with difficult biochemical assays or targets of unknown function...
  38. ncbi Bisphosphonate inhibitors of Toxoplasma gondi growth: in vitro, QSAR, and in vivo investigations
    Yan Ling
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, 2001 South Lincoln Avenue, Urbana, Illinois, 61802, USA
    J Med Chem 48:3130-40. 2005
    ..Overall, these results indicate that alkyl bisphosphonates are promising compounds for further development as agents against Toxoplasma gondii growth, in vivo...
  39. ncbi AFMoC enhances predictivity of 3D QSAR: a case study with DOXP-reductoisomerase
    Katrin Silber
    Department of Pharmaceutical Chemistry, Philipps University, Marbacher Weg 6, 35037 Marburg, Germany
    J Med Chem 48:3547-63. 2005
    ..Using 50% tailored fields was found to permit the precise prediction of binding affinities for related ligands without losing the capability to estimate the affinities of structurally distinct inhibitors...
  40. ncbi Structural basis of fosmidomycin action revealed by the complex with 2-C-methyl-D-erythritol 4-phosphate synthase (IspC). Implications for the catalytic mechanism and anti-malaria drug development
    Stefan Steinbacher
    Max Planck Institut fur Biochemie, Abteilung für Strukturforschung, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Biol Chem 278:18401-7. 2003
    ..Both sites are connected by a spacer of three methylene groups. The substrate molecule, 1-d-deoxyxylulose 5-phosphate, can be superimposed onto fosmidomycin, explaining the stereochemical course of the reaction...
  41. ncbi Stabilization due to dimer formation of phosphoribosyl anthranilate isomerase from Thermus thermophilus HB8: X-ray Analysis and DSC experiments
    Junichiro Taka
    RIKEN Harima Institute at SPring8, 1 1 1 Kohto, Mikazukicho, Sayo, Hyogo 679 5148
    J Biochem 137:569-78. 2005
    ....
  42. ncbi Isoprenoid biosynthesis via the methylerythritol phosphate pathway. Mechanistic investigations of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase
    Jean François Hoeffler
    Université Louis Pasteur CNRS, Institut Le Bel, Strasbourg, France
    Eur J Biochem 269:4446-57. 2002
    ..The equilibrium was, however, largely displaced in favour of the formation of MEP. The reduction step required the presence of a divalent cation such as Mg(2+) or Mn(2+)...
  43. ncbi A fragment-based approach to understanding inhibition of 1-deoxy-D-xylulose-5-phosphate reductoisomerase
    Ludovic Mercklé
    School of Chemistry, University of Bristol, Cantock s Close, Clifton, Bristol, BS8 1TS, UK
    Chembiochem 6:1866-74. 2005
    ..This makes the rational design of new inhibitors of DXR difficult at best...
  44. pmc Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase, the first committed enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway
    Lorenzo Carretero-Paulet
    Departament de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, c Marti i Franques 1, 08028 Barcelona, Spain
    Plant Physiol 129:1581-91. 2002
    ..Our results are consistent with the participation of the Arabidopsis DXR gene in the control of the 2-C-methyl-D-erythritol 4-phosphate pathway...
  45. pmc Novel xylose dehydrogenase in the halophilic archaeon Haloarcula marismortui
    Ulrike Johnsen
    Institut fur Allgemeine Mikrobiologie, Christian Albrechts Universitat Kiel, Am Botanischen Garten 1 9, D 24118 Kiel, Germany
    J Bacteriol 186:6198-207. 2004
    ..marismortui. Thus, we propose that this first characterized archaeal xylose dehydrogenase catalyzes the initial step in xylose degradation by H. marismortui...
  46. ncbi Comparative protein modeling of 1-deoxy-D-xylulose-5-phosphate reductoisomerase enzyme from Plasmodium falciparum: a potential target for antimalarial drug discovery
    Nidhi Singh
    Department of Medicinal Chemistry, Laboratory for Applied Drug Design and Synthesis, University of Mississippi, University, Mississippi 38677 1848, USA
    J Chem Inf Model 46:1360-70. 2006
    ..84. Results of the current study should prove useful in the early design and development of inhibitors by either de novo drug design or virtual screening of large small-molecule databases leading to development of new antimalarial agents...
  47. ncbi Molecular epidemiology of malaria in Cameroon. XXV. In vitro activity of fosmidomycin and its derivatives against fresh clinical isolates of Plasmodium falciparum and sequence analysis of 1-deoxy-D-xylulose 5-phosphate reductoisomerase
    Rachida Tahar
    Unité de Recherche 77 Paludologie Afro tropicale, Institut de Recherche pour le Developpement, Organisation de Coordination pour la Lutte Contre les Endemies en Afrique Centrale, Yaounde, Cameroon
    Am J Trop Med Hyg 77:214-20. 2007
    ..Sequence analysis showed five amino acid substitutions, but their possible relationship with the response to fosmidomycin is not clear. Fosmidomycin derivatives are promising candidates for further development...
  48. ncbi Crystal structure at 2.0 A resolution of phosphoribosyl anthranilate isomerase from the hyperthermophile Thermotoga maritima: possible determinants of protein stability
    M Hennig
    Department of Structural Biology, Biozentrum, University of Basel, Switzerland
    Biochemistry 36:6009-16. 1997
    ..R., Szadkowski, H., Lustig, A., Hennig, M., & Kirschner, K. (1996) Protein Sci. 5, 2000-2008]. The increased number of hydrogen bonds between the phosphate ion and tPRAI compared to ePRAI could be responsible for this effect...
  49. ncbi A new 1-deoxy-D-xylulose 5-phosphate reductoisomerase gene encoding the committed-step enzyme in the MEP pathway from Rauvolfia verticillata
    Zhihua Liao
    Laboratory of Natural Products and Metabolic Engineering, Institute of Biotechnology, Key Laboratory of Eco environments in Three Gorges Reservoir Region Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China
    Z Naturforsch C 62:296-304. 2007
    ..verticillata TIA biosynthesis at the molecular level and provides a candidate gene for metabolic engineering of the TIAs pathway in R. verticillata...
  50. ncbi Kinetic characterization of Synechocystis sp. PCC6803 1-deoxy-D-xylulose 5-phosphate reductoisomerase mutants
    Roberta P M Fernandes
    Department of Pharmaceutical Sciences, College of Pharmacy, Pharmacy Bldg Rm 203, Oregon State University, Corvallis, OR 97331 3507, USA
    Biochim Biophys Acta 1764:223-9. 2006
    ..Alteration of the three acidic residues had major effects on catalysis, changes to S153 and M206 had variable effects on binding and catalysis, and a H155A mutation had only minimal effects on the kinetic parameters...
  51. ncbi Toward Mycobacterium tuberculosis DXR inhibitor design: homology modeling and molecular dynamics simulations
    Nidhi Singh
    Department of Medicinal Chemistry, Laboratory for Applied Drug Design and Synthesis, University of Mississippi, University, MS, 38677 1848, USA
    J Comput Aided Mol Des 21:511-22. 2007
    ....
  52. ncbi A phospho-sugar binding domain homologous to NagB enzymes regulates the activity of the central glycolytic genes repressor
    Thierry Doan
    Microbiologie et Genetique Moleculaire, INRA UMR1238 CNRS UMR2585 AgroParisTech, F 78850 Thiverval Grignon, France
    Proteins 71:2038-50. 2008
    ..Based on these results, we propose that the activity of the CggR-like repressors is controlled by a phospho-sugar binding (PSB) domain presenting structural and functional homology with NagB enzymes...
  53. ncbi Towards new antimalarial drugs: synthesis of non-hydrolyzable phosphate mimics as feed for a predictive QSAR study on 1-deoxy-D-xylulose-5-phosphate reductoisomerase inhibitors
    Dirk Giessmann
    Institute of Pharmacy, Ernst Moritz Arndt University, Friedrich Ludwig Jahn Strasse 17, D 17487 Greifswald
    Chem Biodivers 5:643-56. 2008
    ..Synthetic access to a set of phosphonic acids with inhibitory activity (IC(50)) in the range from 1 to >30 microM vs. E. coli Dxr and 0.4 to 20 microM against P. falciparum Dxr is reported...
  54. ncbi Studies addressing the importance of charge in the binding of fosmidomycin-like molecules to deoxyxylulosephosphate reductoisomerase
    Johann Perruchon
    Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
    ChemMedChem 3:1232-41. 2008
    ..Through occupation of a hydrophobic binding site, some activity could be regained, leading to compounds with micromolar activity against cultured malaria parasites...
  55. pmc (Beta alpha)8-barrel proteins of tryptophan biosynthesis in the hyperthermophile Thermotoga maritima
    R Sterner
    Abteilung fur Biophysikalische Chemie, Universitat Basel, Switzerland
    EMBO J 14:4395-402. 1995
    ..Another notable feature is the predicted lack of the N-terminal helix alpha 0 in the alpha-subunit of tryptophan synthase...
  56. pmc Dxr is essential in Mycobacterium tuberculosis and fosmidomycin resistance is due to a lack of uptake
    Amanda C Brown
    Institute for Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, London, UK
    BMC Microbiol 8:78. 2008
    ..The fact that there is no glpT homologue in the M. tuberculosis genome and the highly impervious nature of the hydrophobic mycobacterial cell wall suggests that resistance may be due to a lack of cellular penetration...
  57. ncbi Structural conservation in parallel beta/alpha-barrel enzymes that catalyze three sequential reactions in the pathway of tryptophan biosynthesis
    M Wilmanns
    Department of Structural Biology, University of Basel, Switzerland
    Biochemistry 30:9161-9. 1991
    ..abstract truncated at 250 words)..
  58. ncbi Three-dimensional structure of the bifunctional enzyme phosphoribosylanthranilate isomerase: indoleglycerolphosphate synthase from Escherichia coli refined at 2.0 A resolution
    M Wilmanns
    Department of Structural Biology, University of Basel, Switzerland
    J Mol Biol 223:477-507. 1992
    ..coli results from a gene duplication event of a monomeric beta/alpha-barrel ancestor...
  59. ncbi The chemical mechanism of D-1-deoxyxylulose-5-phosphate reductoisomerase from Escherichia coli
    Ursula Wong
    School of Chemistry, University of Bristol, Cantock s Close, Bristol, BS8 1TS, UK
    Angew Chem Int Ed Engl 46:4926-9. 2007
  60. ncbi Structures of Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate reductoisomerase provide new insights into catalysis
    Lena M Henriksson
    Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, SE 751 24 Uppsala, Sweden
    J Biol Chem 282:19905-16. 2007
    ..The conformation of fosmidomycin bound to the metal ion is different from that reported in a previously published structure and indicates that a rearrangement of the intermediate is not required during catalysis...
  61. ncbi Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative target of biological tagatose production
    Babu A Manjasetty
    New York Structural GenomiX Research Consortium, Center for Synchrotron Biosciences, National Synchrotron Light Source, Brookhaven National Laboratory, Upton, NY 11973, USA
    J Mol Biol 360:297-309. 2006
    ..Further, the crystal structure of ECAI forms a basis for identifying molecular determinants responsible for isomerization of arabinose to ribulose in vivo and galactose to tagatose in vitro...
  62. ncbi The structure of rhamnose isomerase from Escherichia coli and its relation with xylose isomerase illustrates a change between inter and intra-subunit complementation during evolution
    I P Korndörfer
    Institute of Molecular Biology Howard Hughes Medical Institute and Department of Physics, 1229 University of Oregon, Eugene, OR, 97403 1229, USA
    J Mol Biol 300:917-33. 2000
    ..The available structural data suggest that a metal-mediated hydride-shift mechanism, which is generally favored for xylose isomerase, is also feasible for rhamnose isomerase...
  63. ncbi Characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase, an enzyme involved in isopentenyl diphosphate biosynthesis, and identification of its catalytic amino acid residues
    T Kuzuyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Biol Chem 275:19928-32. 2000
    ..coli DXP reductoisomerase plays an important role(s) in the conversion of DXP to 2-C-methyl-d-erythritol 4-phosphate, and that His(153), His(209), and His(257), in part, associate with DXP binding in the enzyme molecule...
  64. ncbi Isoprenoid biosynthesis in plants - 2C-methyl-D-erythritol-4-phosphate synthase (IspC protein) of Arabidopsis thaliana
    Felix Rohdich
    Lehrstuhl fur Organische Chemie und Biochemie, Technische Universitat Munchen, Garching, Germany
    FEBS J 273:4446-58. 2006
    ..The pH optimum is 8.0. NADH can substitute for NADPH, albeit at a low rate (14% as compared to NADPH). The enzyme catalyzes the reverse reaction at a rate of 2.1 micromol x min(-1) x mg(-1)...
  65. ncbi Isolation of the dxr gene of Zymomonas mobilis and characterization of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase
    S Grolle
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, 52425, Julich, Germany
    FEMS Microbiol Lett 191:131-7. 2000
    ..5 U mg protein(-1). Catalysis of the intramolecular rearrangement and reduction of DXP to MEP is competitively inhibited by the antibiotic fosmidomycin with a K(i) of 0.6 microM...
  66. ncbi Molecular cloning, expression profiling and functional analysis of a DXR gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Camptotheca acuminata
    Hongyan Yao
    Plant Biotechnology Research Center, Shanghai Key Laboratory of Agrobiotechnology, Fudan SJTU Nottingham Plant Biotechnology R and D Center, School of Agriculture and Biology, School of Life Science and Technology, Shanghai Jiao Tong University, Shanghai, PRC
    J Plant Physiol 165:203-13. 2008
    ....
  67. ncbi Tools for discovery of inhibitors of the 1-deoxy-D-xylulose 5-phosphate (DXP) synthase and DXP reductoisomerase: an approach with enzymes from the pathogenic bacterium Pseudomonas aeruginosa
    B Altincicek
    Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, Giessen, Germany
    FEMS Microbiol Lett 190:329-33. 2000
    ..A novel and convenient spectrophotometric assay was developed to determine activity and inhibition of P. aeruginosa DXP synthase. Fluoropyruvate is described as a first inhibitor of DXP synthase...
  68. ncbi Construction and characterization of Escherichia coli disruptants defective in the yaeM gene
    T Kuzuyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    Biosci Biotechnol Biochem 63:776-8. 1999
    ..This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli...
  69. ncbi Anti-malarial drug targets: screening for inhibitors of 2C-methyl-D-erythritol 4-phosphate synthase (IspC protein) in Mediterranean plants
    J Kaiser
    Lehrstuhl fur Organische Chemie und Biochemie, Technische Universitat Munchen, Lichtenbergstr 4, D 85747 Garching, Germany
    Phytomedicine 14:242-9. 2007
    ..Using photometric and NMR spectroscopic assays, we screened extracts of Mediterranean plants for inhibitors of the enzyme. Strongest inhibitory activity was found in leaf extracts of Cercis siliquastrum...
  70. ncbi Biosynthesis of inner core lipopolysaccharide in enteric bacteria identification and characterization of a conserved phosphoheptose isomerase
    J S Brooke
    Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, N6A 5C1 Canada
    J Biol Chem 271:3608-14. 1996
    ..We also demonstrated that lpcA is conserved among enteric bacteria, all of which contain glyceromannoheptose in the inner core LPS, indicating that LpcA is an essential component in a conserved biosynthetic pathway of inner core LPS...
  71. ncbi Crystallographic studies of the mechanism of xylose isomerase
    G K Farber
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139
    Biochemistry 28:7289-97. 1989
    ..In contrast, simple sugar isomerases all require a metal ion and show very low solvent exchange. These observations are rationalized on the basis of the need for stereospecific sugar binding...
  72. pmc Cloning, nucleotide sequence, and overexpression of the L-rhamnose isomerase gene from Pseudomonas stutzeri in Escherichia coli
    Khim Leang
    Department of Biochemistry and Food Science, Faculty of Agriculture and Rare Sugar Research Center, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Appl Environ Microbiol 70:3298-304. 2004
    ..0 to 11.0, with an optimum at pH 9.0...
  73. ncbi Current studies on biological tagatose production using L-arabinose isomerase: a review and future perspective
    Pil Kim
    Division of Biotechnology, The Catholic University of Korea, 43 1 Yokkok2 dong, Wonmi gu, Bucheon, 420 743, South Korea
    Appl Microbiol Biotechnol 65:243-9. 2004
    ..The industrial problems facing its commercial application is described and evolving potential solutions are suggested...
  74. ncbi Identification of two cysteine residues forming a pair of vicinal thiols in glucosamine-6-phosphate deaminase from Escherichia coli and a study of their functional role by site-directed mutagenesis
    M M Altamirano
    Departamento de Bioquimica, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, D F
    Biochemistry 31:1153-8. 1992
    ..However, only one of these cysteinyl residues, Cys239, had a significant role in the allosteric transition, and its substitution for serine reduced the allosteric interaction energy, due to a lower value of KT...
  75. ncbi A microfluidic device for kinetic optimization of protein crystallization and in situ structure determination
    Carl L Hansen
    Department of Applied Physics, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, USA
    J Am Chem Soc 128:3142-3. 2006
    ....
  76. ncbi Computational analysis of the evolution of 1-deoxy-D-xylulose-5-phosphate Reductoisomerase, an important enzyme in plant terpene biosynthesis
    Pui Kwan Fung
    Department Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA 01609, USA
    Chem Biodivers 7:1098-110. 2010
    ..These features include a twin arginine motif, a hydrophobic region, and a proline-rich region. The transit peptide also showed putative motifs for a 14-3-3 binding site with a chaperone phosphorylation site at Thr...
  77. ncbi The malarial drug target Plasmodium falciparum 1-deoxy-D-xylulose-5-phosphate reductoisomerase (PfDXR): development of a 3-D model for identification of novel, structural and functional features and for inhibitor screening
    Jessica L Goble
    Biomedical Biotechnology Research Unit, Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, Grahamstown 6140, South Africa
    Protein Pept Lett 17:109-20. 2010
    ..Furthermore, we have used the model to develop an efficient approach to screen for potential tool compounds for use in the rational design of novel DXR inhibitors...
  78. ncbi Substrate specificity of ribose-5-phosphate isomerases from Clostridium difficile and Thermotoga maritima
    Soo Jin Yeom
    Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang dong, Gwangjin gu, Seoul, 143 701, South Korea
    Biotechnol Lett 32:829-35. 2010
    ..In contrast, T. maritima RpiB displayed activity only with aldose substrates possessing hydroxyl groups configured the same direction at the C2, C3, and C4 positions, such as the D- and L-forms of ribose, talose, and allose...
  79. pmc Functional genetic analysis of the Plasmodium falciparum deoxyxylulose 5-phosphate reductoisomerase gene
    Audrey R Odom
    Department of Pediatrics, Division of Infectious Diseases, Washington University School of Medicine, 660 S Euclid Ave, Campus Box 8208, St Louis, MO 63110, USA
    Mol Biochem Parasitol 170:108-11. 2010
    ..These data indicate that DXR is required for intraerythrocytic development of P. falciparum...
  80. ncbi Kinetic and enzymatic adsorption model in a recirculation hollow-fibre bioreactor
    E Jurado
    Departamento Ingeniería Química, Facultad Ciencias, Universidad de Granada, Campus Universitario Fuentenueva, Avda Fuentenueva, S N, 18071 Granada, Spain
    Bioprocess Biosyst Eng 28:27-36. 2005
    ....
  81. pmc Structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase in a quaternary complex with a magnesium ion, NADPH and the antimalarial drug fosmidomycin
    Shunsuke Yajima
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:466-70. 2007
    ..On the other hand, no electron density was observed for the nicotinamide-ribose portion of NADPH and the position of Asp149 anchoring Mg(2+) was shifted by NADPH in the active site...
  82. ncbi Identification and characterization of key substructures involved in the early folding events of a (beta/alpha)8-barrel protein as studied by experimental and computational methods
    Satoshi Akanuma
    Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    J Mol Biol 353:1161-70. 2005
    ..Interactions found in (beta/alpha)(3-4)beta5 may be essential for the early events of ePRAI folding if they provide a nucleation site that directs folding...
  83. ncbi The structure/function relationship of a dual-substrate (betaalpha)8-isomerase
    Helena Wright
    Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK
    Biochem Biophys Res Commun 365:16-21. 2008
    ..This represents the first report of the structure/function relationship of this (betaalpha)8-isomerase...
  84. ncbi Heterologous expression of the alcohol dehydrogenase (adhI) gene from Geobacillus thermoglucosidasius strain M10EXG
    Young Jae Jeon
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney 2052, Australia
    J Biotechnol 135:127-33. 2008
    ..In silico analysis, including phylogenetic reconstruction and protein modeling, confirmed that the thermostable enzyme from G. thermoglucosidasius is likely to belong to the NAD-Zn-dependent family of alcohol dehydrogenases...
  85. ncbi Thermoinactivation mechanism of glucose isomerase
    Leng Hong Lim
    Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, M5S 3E5, Toronto, Ontario
    Appl Biochem Biotechnol 137:115-30. 2007
    ..At 60 and 80 degrees C, IGI had higher thermostability in continuous reactors than in batch reactors, possibly because of reduced contact with deleterious compounds in HFCS...
  86. ncbi The crystal structure of 5-keto-4-deoxyuronate isomerase from Escherichia coli
    Robert L Crowther
    The Waksman Institute, Rutgers University, Piscataway, New Jersey, USA
    Proteins 61:680-4. 2005
  87. pmc Crystallization and preliminary X-ray analysis of methylthioribose-1-phosphate isomerase from Bacillus subtilis
    Haruka Tamura
    Department of Materials Chemistry, Graduate School of Engineering, Osaka University, 2 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:595-8. 2005
    ..2, c = 154.7 A. The asymmetric unit contains two molecules of MtnA, with a VM value of 2.4 A3 Da(-1) and a solvent content of 48%...
  88. ncbi Coordination chemistry based approach to lipophilic inhibitors of 1-deoxy-D-xylulose-5-phosphate reductoisomerase
    Lisheng Deng
    Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Med Chem 52:6539-42. 2009
    ..4 microM. It exhibited a broad spectrum of activity against Gram-negative and -positive bacteria with minimal inhibition concentrations of 20-100 microM (or 3.7-19 microg/mL)...
  89. ncbi A detailed unfolding pathway of a (beta/alpha)8-barrel protein as studied by molecular dynamics simulations
    Satoshi Akanuma
    Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
    Proteins 58:538-46. 2005
    ..Our simulations also predicted the presence of a nucleation site, onto which several hydrophobic residues condensed forming the foundation for the central betaalphabetaalphabeta module...
  90. ncbi Ring opening is not rate-limiting in the GTP cyclohydrolase I reaction
    N Schramek
    Lehrstuhl fur Organische Chemie und Biochemie, Technische Universitat Munchen, Lichtenbergstrasse 4, D 85747 Garching, Germany
    J Biol Chem 276:2622-6. 2001
    ..0 s(-1), and the rate constant k(4) for the formation of dihydroneopterin triphosphate was 0.03 s(-1). Thus, the hydrolytic opening of the imidazole ring of GTP is rapid by comparison with the overall reaction...
  91. ncbi Comparison of backbone structures of glucose isomerase from Streptomyces and Arthrobacter
    K Henrick
    Blackett Laboratory, Imperial College, London, UK
    Protein Eng 1:467-9. 1987
    ..There is a close structural homology throughout the whole molecule, which is most accurate up to position 325. The r.m.s. displacement for 315 homologous C alpha positions up to this position is 0.92 A...
  92. ncbi Automatic classification of sub-microlitre protein-crystallization trials in 1536-well plates
    Christian A Cumbaa
    Ontario Cancer Institute, 610 University Avenue, Toronto, Ontario M5G 2M9, Canada
    Acta Crystallogr D Biol Crystallogr 59:1619-27. 2003
    ..Many images falsely classified as crystal-negative variously contain very fine crystal features or dendrites lacking straight edges. Characterization of these misclassifications suggests directions for improving the method...
  93. ncbi Mapping arm-DNA-binding domain interactions in AraC
    M Wu
    Biology Department, Johns Hopkins University, 3400 N Charles St, Baltimore, MD, 21218, USA
    J Mol Biol 307:1001-9. 2001
    ..They form a contiguous trail on the DNA-distal face of the DNA-binding domain, and likely define the region where the N-terminal arm that extends from the N-terminal dimerization domain contacts the C-terminal DNA-binding domain...
  94. pmc Sequencing and characterization of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli
    P Moralejo
    Department of Biochemistry, School of Pharmacy, University of Barcelona, Spain
    J Bacteriol 175:5585-94. 1993
    ..The rhaB transcription start site was mapped to -24 relative to the start of translation. Mutations in the catabolic genes were used to show that L-rhamnose may directly induce rhaBAD transcription...
  95. pmc Three-dimensional profiles from residue-pair preferences: identification of sequences with beta/alpha-barrel fold
    M Wilmanns
    Molecular Biology Institute, University of California, Los Angeles 90024 1570
    Proc Natl Acad Sci U S A 90:1379-83. 1993
    ....
  96. pmc Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene
    L Bartee
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    Genes Dev 15:1753-8. 2001
    ..Thus, CMT3 is a key determinant for non-CG methylation. The lack of CMT homologs in animal genomes could account for the observation that in contrast to plants, animals maintain primarily CG methylation...
  97. ncbi Two (betaalpha)(8)-barrel enzymes of histidine and tryptophan biosynthesis have similar reaction mechanisms and common strategies for protecting their labile substrates
    Martina Henn-Sax
    Institut fur Biochemie, Universitat zu Koln, Otto Fischer Strasse 12 14, D 50674 Köln, Germany
    Biochemistry 41:12032-42. 2002
    ..These results suggest that HisA and TrpF have similar chemical reaction mechanisms and use the same strategy to prevent the loss of their thermolabile substrates...
  98. pmc The chromosome bias of misincorporations during double-strand break repair is not altered in mismatch repair-defective strains of Saccharomyces cerevisiae
    C B McGill
    Gene Regulation and Chromosome Biology Laboratory, National Cancer Institute Frederick Cancer Research and Development Center, ABL Basic Research Program, Maryland 21702 1201, USA
    Genetics 148:1525-33. 1998
    ..The results suggest that mismatch repair is not responsible for the observed bias...
  99. ncbi Sequence analysis of D-xylose isomerase gene from Escherichia coli
    Y M Hou
    Institute of Biophysic, Chinese Academy of Sciences, Beijing
    Chin J Biotechnol 6:269-77. 1990
    ..The additional 209 nucleotides at 5' end and 82 nucleotides at 3' end include Shine-Dalgarno ribosome-binding site and the transcriptional termination codon, respectively...
  100. ncbi Diaryl ester prodrugs of FR900098 with improved in vivo antimalarial activity
    A Reichenberg
    Jomaa Pharmaka GmbH, Giessen, Germany
    Bioorg Med Chem Lett 11:833-5. 2001
    ..One diaryl ester prodrug demonstrated efficacy in mice infected with the rodent malaria parasite Plasmodium vinckei comparable to i.p. drug administration...
  101. ncbi Numerical calculations of the pH of maximal protein stability. The effect of the sequence composition and three-dimensional structure
    Emil Alexov
    Howard Hughes Medical Institute and Columbia University, Biochemistry Department, New York 10032, USA
    Eur J Biochem 271:173-85. 2004
    ..It was shown that a protein that provides a favorable electrostatic environment for acids and disfavors the bases tends to have high optimum pH and vice versa...

Research Grants2

  1. GENETIC ANALYSIS OF ENZYME PROCESSING AND LOCALIZATION
    Elizabeth Jones; Fiscal Year: 2002
    ..Methods will include targeted mutagenesis, suppressor selection and analysis, subcellular fractionation, biochemistry, and immunofluorescence. ..