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| aldose ketose isomerasesSummarySummary: Enzymes that catalyze the interconversion of aldoses and ketoses. EC 5.3.1. Top Publications
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Publications
The 1.9 A resolution structure of Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a potential drug targetLena M Henriksson
Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, SE 751 24 Uppsala, Sweden
Acta Crystallogr D Biol Crystallogr 62:807-13. 2006..Furthermore, the new structure represents an intermediate conformation between the open apo form and the closed holo form observed previously, giving insights into the conformational changes associated with catalysis...
Modulation of carbohydrate metabolism during N-methyl N-nitrosourea induced neurotoxicity in mice: role of curcuminNeha Singla
Department of Biophysics, Nuclear Medicine, Panjab University, Chandigarh, India
Neurochem Res 35:660-5. 2010..Hence, curcumin shall prove to be effective in ameliorating the adverse effects caused by MNU...- Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugsH Jomaa
Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, Friedrichstrasse 24, D 35392 Giessen, Germany
Science 285:1573-6. 1999..Both drugs suppressed the in vitro growth of multidrug-resistant P. falciparum strains. After therapy with these drugs, mice infected with the rodent malaria parasite P. vinckei were cured... 
1-Deoxy-D-xylulose 5-phosphate reductoisomerase (IspC) from Mycobacterium tuberculosis: towards understanding mycobacterial resistance to fosmidomycinRakesh K Dhiman
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
J Bacteriol 187:8395-402. 2005..Thus, M. tuberculosis resistance to fosmidomycin is not due to intrinsic properties of Rv2870c, and the enzyme appears to be a valid drug target in this pathogen...- Evaluation of fosmidomycin analogs as inhibitors of the Synechocystis sp. PCC6803 1-deoxy-D-xylulose 5-phosphate reductoisomeraseYoun-Hi Woo
Department of Pharmaceutical Sciences, College of Pharmacy, Pharmacy Bldg. Rm. 203, Oregon State University, Corvallis, OR 97331-3507, USA
Bioorg Med Chem 14:2375-85. 2006..PCC6803 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR). Fosfoxacin, the phosphate analog of fosmidomycin, and its acetyl congener were found to be more potent inhibitors of DXR than fosmidomycin... - The crystal structure of E.coli 1-deoxy-D-xylulose-5-phosphate reductoisomerase in a ternary complex with the antimalarial compound fosmidomycin and NADPH reveals a tight-binding closed enzyme conformationAengus Mac Sweeney
Morphochem AG, WRO 1055 338, Schwarzwaldallee 215, CH 4058, Basel, Switzerland
J Mol Biol 345:115-27. 2005..The structure of the substrate complex must be interpreted with caution due to the presence of a second diastereomer in the active site... - Structure and mechanism of L-fucose isomerase from Escherichia coliJ E Seemann
Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Freiburg im Breisgau, Germany
J Mol Biol 273:256-68. 1997..Most likely, L-fucitol mimics a bound L-fucose molecule in its open chain form. The protein environment suggests strongly that the reaction belongs to the ene-diol type... - Targeting the methyl erythritol phosphate (MEP) pathway for novel antimalarial, antibacterial and herbicidal drug discovery: inhibition of 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) enzymeNidhi Singh
Department of Medicinal Chemistry and Laboratory for Applied Drug Design and Synthesis, School of Pharmacy, University of Mississippi, University, MS 38677 1848, USA
Curr Pharm Des 13:1161-77. 2007.... - Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomeraseC Vieille
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA
Eur J Biochem 268:6291-301. 2001..Thus, it would appear that protein thermostability is a function of more complex molecular determinants than amino-acid content alone... - Novel deoxyxylulosephosphate-reductoisomerase inhibitors: fosmidomycin derivatives with spacious acyl residuesRegina Ortmann
Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marburg, Germany
Arch Pharm (Weinheim) 340:483-90. 2007.... - Dissolution of xylose metabolism in Lactococcus lactisK A Erlandson
Department of Food Science, Cornell University, Ithaca, New York 14853, USA
Appl Environ Microbiol 66:3974-80. 2000..Nevertheless, either cumulatively or because of indirect affects on the structures of catalytic sites, these mutations render some strains of L. lactis unable to metabolize xylose... - Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: implications for catalytic mechanismHaruka Tamura
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Osaka, Japan
Protein Sci 17:126-35. 2008..The highly conserved residues at the active site, namely, Cys160 and Asp240 are most likely to be involved in catalysis. The structural analysis sheds light on its catalytic mechanism of M1Pi... - Regulation of expression, genetic organization and substrate specificity of xylose uptake in Bacillus megateriumD Schmiedel
Lehrstuhl fur Mikrobiologie, Institut fur Mikrobiologie, Biochemie und Genetik, Friedrich Alexander Universitat Erlangen Nurnberg, Germany
Mol Microbiol 23:1053-62. 1997..XylT has an apparent Michaelis constant (KM) of approx. 100 microM and is competitively inhibited by glucose with an inhibitor constant KI of 16 mM... - Crystal structure of D-ribose-5-phosphate isomerase (RpiA) from Escherichia coliErumbi S Rangarajan
Department of Biochemistry, McGill University, Montreal, Quebec, Canada
Proteins 48:737-40. 2002 - Restoration of a defective Lactococcus lactis xylose isomeraseJoo-Heon Park
Department of Food Science, Cornell University, Ithaca, NY 14853, USA
Appl Environ Microbiol 70:4318-25. 2004..The dissolution of XI activity in L. lactis subsp. lactis involves a series of mutations that collectively eliminate enzyme activity by reducing the solubility of the enzyme... - Synthesis and evaluation of 1-deoxy-D-xylulose 5-phosphate analogues as chelation-based inhibitors of methylerythritol phosphate synthaseJoel R Walker
Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
J Org Chem 70:9955-9. 2005..The carboxylate (1), methyl ester (3), amide (4), and alcohol (5) analogues were inhibitors with IC50's ranging from 0.25 to 1.0 mM. The hydroxamic acid (2) and amino (6) analogues did not inhibit the enzyme... - Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus licheniformis encoded regulon for xylose utilizationA Scheler
Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Erlangen, Federal Republic of Germany
Arch Microbiol 155:526-34. 1991..The next greater differences are found to the S. xylosus and the greatest to the E. coli encoded genes. These results are discussed with respect to the taxonomic relations of these bacteria... - Crystallographic structures of two bisphosphonate:1-deoxyxylulose-5-phosphate reductoisomerase complexesShunsuke Yajima
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
J Am Chem Soc 126:10824-5. 2004..The availability of these two new crystal structures opens up the possibility of the further development of bisphosphonates and related systems as DXR inhibitors and, potentially, as antiinfective agents... - Crystal structure of yeast Ypr118w, a methylthioribose-1-phosphate isomerase related to regulatory eIF2B subunitsMario Bumann
Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, Berne CH 3012
J Biol Chem 279:37087-94. 2004.... - Xylose isomerase from Escherichia coli. Characterization of the protein and the structural geneG D Schellenberg
J Biol Chem 259:6826-32. 1984..These results establish that the NH2-terminal methionine of xylose isomerase is specified by an ATG which is 7 nucleotides downstream from a Shine-Dalgarno sequence... - Characterization of native and histidine-tagged deoxyxylulose 5-phosphate reductoisomerase from the cyanobacterium Synechocystis sp. PCC6803Xihou Yin
College of Pharmacy, Pharmacy Building, Room 203, Oregon State University, Corvallis, OR 97331-3507, USA
Biochim Biophys Acta 1652:75-81. 2003..The K(m)'s for the metal ions Mn(2+), Mg(2+), and Co(2+) were determined for native DXR for the first time, with the K(m) for Mg(2+) being approximately 200-fold higher than the K(m)'s for Mn(2+) and Co(2+)... - A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesisS Takahashi
Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
Proc Natl Acad Sci U S A 95:9879-84. 1998..coli... - The 2.2 A resolution structure of RpiB/AlsB from Escherichia coli illustrates a new approach to the ribose-5-phosphate isomerase reactionRong guang Zhang
Biosciences Division, Structural Biology Center, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, USA
J Mol Biol 332:1083-94. 2003..The sequence and structural results further suggest that the two homologous components of LacAB (galactose-6-phosphate isomerase) will compose a bi-functional enzyme; the second activity is unknown... - Identification of class 2 1-deoxy-D-xylulose 5-phosphate synthase and 1-deoxy-D-xylulose 5-phosphate reductoisomerase genes from Ginkgo biloba and their transcription in embryo culture with respect to ginkgolide biosynthesisSang-Min Kim
School of Agricultural Biotechnology, Seoul National University, Korea
Planta Med 72:234-40. 2006..Exclusive transcription of ginkgolide biosynthesis-specific LPS and GbDXS2 in roots and the appearance of ginkgolides in leaves was consistent with translocation of the compounds from roots to leaves... - Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic studyRodrigo Arreola
Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, PO Box 510 3, Cuernavaca, 62250 Morelos, Mexico
FEBS Lett 551:63-70. 2003..These structural differences can be related to the kinetic and allosteric properties of both mammalian enzymes... - High-throughput screen for inhibitors of 1-deoxy-d-xylulose 5-phosphate reductoisomerase by surrogate ligand competitionElizabeth B Gottlin
Karo Bio USA, Inc, Durham, NC, USA
J Biomol Screen 8:332-9. 2003..The results presented here suggest that peptide surrogate ligands may be useful in formatting HTS for proteins with difficult biochemical assays or targets of unknown function... - Anti-malarial drug targets: screening for inhibitors of 2C-methyl-D-erythritol 4-phosphate synthase (IspC protein) in Mediterranean plantsJ Kaiser
Lehrstuhl fur Organische Chemie und Biochemie, Technische Universitat Munchen, Lichtenbergstr 4, D 85747 Garching, Germany
Phytomedicine 14:242-9. 2007..Using photometric and NMR spectroscopic assays, we screened extracts of Mediterranean plants for inhibitors of the enzyme. Strongest inhibitory activity was found in leaf extracts of Cercis siliquastrum... - Structural basis of fosmidomycin action revealed by the complex with 2-C-methyl-D-erythritol 4-phosphate synthase (IspC). Implications for the catalytic mechanism and anti-malaria drug developmentStefan Steinbacher
Max Planck Institut fur Biochemie, Abteilung für Strukturforschung, Am Klopferspitz 18a, D 82152 Martinsried, Germany
J Biol Chem 278:18401-7. 2003..Both sites are connected by a spacer of three methylene groups. The substrate molecule, 1-d-deoxyxylulose 5-phosphate, can be superimposed onto fosmidomycin, explaining the stereochemical course of the reaction... - Isoprenoid biosynthesis via the methylerythritol phosphate pathway. Mechanistic investigations of the 1-deoxy-D-xylulose 5-phosphate reductoisomeraseJean François Hoeffler
Université Louis Pasteur CNRS, Institut Le Bel, Strasbourg, France
Eur J Biochem 269:4446-57. 2002..The equilibrium was, however, largely displaced in favour of the formation of MEP. The reduction step required the presence of a divalent cation such as Mg(2+) or Mn(2+)... - Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily?Florence Vincent
Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, UK
J Biol Chem 280:19649-55. 2005..The structure completes the NagB superfamily structural landscape and thus allows further interrogation of genomic data in terms of the regulation of NagB and the metabolic fate(s) of glucosamine 6-phosphate... - Structural conservation in parallel beta/alpha-barrel enzymes that catalyze three sequential reactions in the pathway of tryptophan biosynthesisM Wilmanns
Department of Structural Biology, University of Basel, Switzerland
Biochemistry 30:9161-9. 1991..abstract truncated at 250 words).. - Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase, the first committed enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathwayLorenzo Carretero-Paulet
Departament de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, c Marti i Franques 1, 08028 Barcelona, Spain
Plant Physiol 129:1581-91. 2002..Our results are consistent with the participation of the Arabidopsis DXR gene in the control of the 2-C-methyl-D-erythritol 4-phosphate pathway... - Structures of Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate reductoisomerase provide new insights into catalysisLena M Henriksson
Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, SE 751 24 Uppsala, Sweden
J Biol Chem 282:19905-16. 2007..The conformation of fosmidomycin bound to the metal ion is different from that reported in a previously published structure and indicates that a rearrangement of the intermediate is not required during catalysis... - Antisense and chemical suppression of the nonmevalonate pathway affects ent-kaurene biosynthesis in ArabidopsisKazunori Okada
Department of Biology, Tokyo Gakugei University, Nukuikitamachi 4 1 1, Koganei Shi, Tokyo, 184 8501, Japan
Planta 215:339-44. 2002..These observations suggest that both AtMECT and DXR are important in the synthesis of isopentenyl diphosphate and dimethylallyl diphosphate and that ent-kaurene is mainly produced through the nonmevalonate pathway in the plastid... - Structure of 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase from Shewanella oneidensis at 1.6 A: identification of farnesyl pyrophosphate trapped in a hydrophobic cavityShuisong Ni
Pacific Northwest National Laboratory, Biological Sciences Division, Richland, WA 99352, USA
Acta Crystallogr D Biol Crystallogr 60:1949-57. 2004.... - (Beta alpha)8-barrel proteins of tryptophan biosynthesis in the hyperthermophile Thermotoga maritimaR Sterner
Abteilung fur Biophysikalische Chemie, Universitat Basel, Switzerland
EMBO J 14:4395-402. 1995..Another notable feature is the predicted lack of the N-terminal helix alpha 0 in the alpha-subunit of tryptophan synthase... - A fragment-based approach to understanding inhibition of 1-deoxy-D-xylulose-5-phosphate reductoisomeraseLudovic Mercklé
School of Chemistry, University of Bristol, Cantock s Close, Clifton, Bristol, BS8 1TS, UK
Chembiochem 6:1866-74. 2005..This makes the rational design of new inhibitors of DXR difficult at best... - Synthesis and biological evaluation of cyclopropyl analogues of fosmidomycin as potent Plasmodium falciparum growth inhibitorsVincent Devreux
Laboratory for Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Gent, Belgium
J Med Chem 49:2656-60. 2006..coli DOXP reductoisomerase and proved equally active when tested in vitro for P. falciparum growth inhibition. Conversely, the alpha-phenyl cis-cyclopropyl analogue 4 showed virtually no inhibition of the enzyme... - Comparative protein modeling of 1-deoxy-D-xylulose-5-phosphate reductoisomerase enzyme from Plasmodium falciparum: a potential target for antimalarial drug discoveryNidhi Singh
Department of Medicinal Chemistry, Laboratory for Applied Drug Design and Synthesis, University of Mississippi, University, Mississippi 38677-1848, USA
J Chem Inf Model 46:1360-70. 2006..84. Results of the current study should prove useful in the early design and development of inhibitors by either de novo drug design or virtual screening of large small-molecule databases leading to development of new antimalarial agents... - Kinetic and chemical mechanism of Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate isomeroreductaseArgyrides Argyrou
Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
Biochemistry 43:4375-84. 2004..The results are discussed in terms of significant differences in the commitment factors for the various metal ions and pyridine nucleotides... - Crystal structure of 1-deoxy-d-xylulose-5-phosphate reductoisomerase from Zymomonas mobilis at 1.9-A resolutionStefano Ricagno
Department of Medical Biochemistry and Biophysics, Division of Molecular Structural Biology, Karolinska Institutet, Scheelevagen 2, S 171 77 Stockholm, Sweden
Biochim Biophys Acta 1698:37-44. 2004..However, there are differences in the recognition of the adenine ring of NADPH in the two enzymes... - Novel xylose dehydrogenase in the halophilic archaeon Haloarcula marismortuiUlrike Johnsen
Institut fur Allgemeine Mikrobiologie, Christian Albrechts Universitat Kiel, Am Botanischen Garten 1 9, D 24118 Kiel, Germany
J Bacteriol 186:6198-207. 2004..marismortui. Thus, we propose that this first characterized archaeal xylose dehydrogenase catalyzes the initial step in xylose degradation by H. marismortui... - Isoprenoid biosynthesis in plants - 2C-methyl-D-erythritol-4-phosphate synthase (IspC protein) of Arabidopsis thalianaFelix Rohdich
Lehrstuhl fur Organische Chemie und Biochemie, Technische Universitat Munchen, Garching, Germany
FEBS J 273:4446-58. 2006..The pH optimum is 8.0. NADH can substitute for NADPH, albeit at a low rate (14% as compared to NADPH). The enzyme catalyzes the reverse reaction at a rate of 2.1 micromol x min(-1) x mg(-1)... - Inhibition of isoprene biosynthesis pathway enzymes by phosphonates, bisphosphonates, and diphosphatesFeng Cheng
Departments of Chemistry and Biophysics, 600 South Mathews Avenue, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
J Med Chem 47:5149-58. 2004.... - Three-dimensional structure of the bifunctional enzyme phosphoribosylanthranilate isomerase: indoleglycerolphosphate synthase from Escherichia coli refined at 2.0 A resolutionM Wilmanns
Department of Structural Biology, University of Basel, Switzerland
J Mol Biol 223:477-507. 1992..coli results from a gene duplication event of a monomeric beta/alpha-barrel ancestor... - Molecular cloning, expression profiling and functional analysis of a DXR gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Camptotheca acuminataHongyan Yao
Plant Biotechnology Research Center, Shanghai Key Laboratory of Agrobiotechnology, Fudan SJTU Nottingham Plant Biotechnology R and D Center, School of Agriculture and Biology, School of Life Science and Technology, Shanghai Jiao Tong University, Shanghai, PRC
J Plant Physiol 165:203-13. 2008.... - The chemical mechanism of D-1-deoxyxylulose-5-phosphate reductoisomerase from Escherichia coliUrsula Wong
School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, UK
Angew Chem Int Ed Engl 46:4926-9. 2007 - Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase complexed with cofactors: implications of a flexible loop movement upon substrate bindingShunsuke Yajima
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
J Biochem 131:313-7. 2002..A flexible loop covering the substrate binding site plays an important role in the enzymatic reaction and the determination of substrate specificity... - Bisphosphonate inhibitors of Toxoplasma gondi growth: in vitro, QSAR, and in vivo investigationsYan Ling
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana-Champaign, 2001 South Lincoln Avenue, Urbana, Illinois, 61802, USA
J Med Chem 48:3130-40. 2005..Overall, these results indicate that alkyl bisphosphonates are promising compounds for further development as agents against Toxoplasma gondii growth, in vivo... - The structure of rhamnose isomerase from Escherichia coli and its relation with xylose isomerase illustrates a change between inter and intra-subunit complementation during evolutionI P Korndörfer
Institute of Molecular Biology Howard Hughes Medical Institute and Department of Physics, 1229 University of Oregon, Eugene, OR, 97403 1229, USA
J Mol Biol 300:917-33. 2000..The available structural data suggest that a metal-mediated hydride-shift mechanism, which is generally favored for xylose isomerase, is also feasible for rhamnose isomerase... - Characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase, an enzyme involved in isopentenyl diphosphate biosynthesis, and identification of its catalytic amino acid residuesT Kuzuyama
Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
J Biol Chem 275:19928-32. 2000..coli DXP reductoisomerase plays an important role(s) in the conversion of DXP to 2-C-methyl-d-erythritol 4-phosphate, and that His(153), His(209), and His(257), in part, associate with DXP binding in the enzyme molecule... - A new 1-deoxy-D-xylulose 5-phosphate reductoisomerase gene encoding the committed-step enzyme in the MEP pathway from Rauvolfia verticillataZhihua Liao
Laboratory of Natural Products and Metabolic Engineering, Institute of Biotechnology, Key Laboratory of Eco environments in Three Gorges Reservoir Region Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China
Z Naturforsch C 62:296-304. 2007..verticillata TIA biosynthesis at the molecular level and provides a candidate gene for metabolic engineering of the TIAs pathway in R. verticillata... - Stabilization due to dimer formation of phosphoribosyl anthranilate isomerase from Thermus thermophilus HB8: X-ray Analysis and DSC experimentsJunichiro Taka
RIKEN Harima Institute at SPring8, 1 1 1 Kohto, Mikazukicho, Sayo, Hyogo 679 5148
J Biochem 137:569-78. 2005.... - Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative target of biological tagatose productionBabu A Manjasetty
New York Structural GenomiX Research Consortium, Center for Synchrotron Biosciences, National Synchrotron Light Source, Brookhaven National Laboratory, Upton, NY 11973, USA
J Mol Biol 360:297-309. 2006..Further, the crystal structure of ECAI forms a basis for identifying molecular determinants responsible for isomerization of arabinose to ribulose in vivo and galactose to tagatose in vitro... - Towards new antimalarial drugs: synthesis of non-hydrolyzable phosphate mimics as feed for a predictive QSAR study on 1-deoxy-D-xylulose-5-phosphate reductoisomerase inhibitorsDirk Giessmann
Institute of Pharmacy, Ernst Moritz Arndt University, Friedrich Ludwig Jahn Strasse 17, D 17487 Greifswald
Chem Biodivers 5:643-56. 2008..Synthetic access to a set of phosphonic acids with inhibitory activity (IC(50)) in the range from 1 to >30 microM vs. E. coli Dxr and 0.4 to 20 microM against P. falciparum Dxr is reported... - Construction and characterization of Escherichia coli disruptants defective in the yaeM geneT Kuzuyama
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
Biosci Biotechnol Biochem 63:776-8. 1999..This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli... - Studies addressing the importance of charge in the binding of fosmidomycin-like molecules to deoxyxylulosephosphate reductoisomeraseJohann Perruchon
Institut fur Pharmazeutische Chemie, Philipps Universitat Marburg, Marbacher Weg 6, 35032 Marburg, Germany
ChemMedChem 3:1232-41. 2008..Through occupation of a hydrophobic binding site, some activity could be regained, leading to compounds with micromolar activity against cultured malaria parasites... - Crystal structure at 2.0 A resolution of phosphoribosyl anthranilate isomerase from the hyperthermophile Thermotoga maritima: possible determinants of protein stabilityM Hennig
Department of Structural Biology, Biozentrum, University of Basel, Switzerland
Biochemistry 36:6009-16. 1997..R., Szadkowski, H., Lustig, A., Hennig, M., & Kirschner, K. (1996) Protein Sci. 5, 2000-2008]. The increased number of hydrogen bonds between the phosphate ion and tPRAI compared to ePRAI could be responsible for this effect... - Dxr is essential in Mycobacterium tuberculosis and fosmidomycin resistance is due to a lack of uptakeAmanda C Brown
Institute for Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, London, UK
BMC Microbiol 8:78. 2008..CONCLUSION: Taken together, these data demonstrate that fosmidomycin resistance in M. tuberculosis and M. smegmatis results from a lack of penetration of the antibiotic to the site of the sensitive target... - Tools for discovery of inhibitors of the 1-deoxy-D-xylulose 5-phosphate (DXP) synthase and DXP reductoisomerase: an approach with enzymes from the pathogenic bacterium Pseudomonas aeruginosaB Altincicek
Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, Giessen, Germany
FEMS Microbiol Lett 190:329-33. 2000..A novel and convenient spectrophotometric assay was developed to determine activity and inhibition of P. aeruginosa DXP synthase. Fluoropyruvate is described as a first inhibitor of DXP synthase... - Isolation of the dxr gene of Zymomonas mobilis and characterization of the 1-deoxy-D-xylulose 5-phosphate reductoisomeraseS Grolle
Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, 52425, Julich, Germany
FEMS Microbiol Lett 191:131-7. 2000..5 U mg protein(-1). Catalysis of the intramolecular rearrangement and reduction of DXP to MEP is competitively inhibited by the antibiotic fosmidomycin with a K(i) of 0.6 microM... - A phospho-sugar binding domain homologous to NagB enzymes regulates the activity of the central glycolytic genes repressorThierry Doan
Microbiologie et Genetique Moleculaire, INRA UMR1238 CNRS UMR2585 AgroParisTech, F 78850 Thiverval Grignon, France
Proteins 71:2038-50. 2008..Based on these results, we propose that the activity of the CggR-like repressors is controlled by a phospho-sugar binding (PSB) domain presenting structural and functional homology with NagB enzymes... - On the multiple functional roles of the active site histidine in catalysis and allosteric regulation of Escherichia coli glucosamine 6-phosphate deaminaseG M Montero-Morán
Departamento de Bioquimica, Laboratorio de Fisicoquímica y Diseño de Proteínas, Facultad de Medicina, Universidad Nacional Autonoma de Mexico UNAM, P O Box 70 159, Mexico City 04510, D F, Mexico
Biochemistry 40:10187-96. 2001..This, in turn, indicates that the interaction Glu148-His143 in the wild-type enzyme in the R state contributes to make the enzyme functional over a wide pH range... - Structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase in a quaternary complex with a magnesium ion, NADPH and the antimalarial drug fosmidomycinShunsuke Yajima
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo, Japan
Acta Crystallogr Sect F Struct Biol Cryst Commun 63:466-70. 2007..On the other hand, no electron density was observed for the nicotinamide-ribose portion of NADPH and the position of Asp149 anchoring Mg(2+) was shifted by NADPH in the active site... - Molecular epidemiology of malaria in Cameroon. XXV. In vitro activity of fosmidomycin and its derivatives against fresh clinical isolates of Plasmodium falciparum and sequence analysis of 1-deoxy-D-xylulose 5-phosphate reductoisomeraseRachida Tahar
Unité de Recherche 77 Paludologie Afro tropicale, Institut de Recherche pour le Developpement, Organisation de Coordination pour la Lutte Contre les Endemies en Afrique Centrale, Yaounde, Cameroon
Am J Trop Med Hyg 77:214-20. 2007..Sequence analysis showed five amino acid substitutions, but their possible relationship with the response to fosmidomycin is not clear. Fosmidomycin derivatives are promising candidates for further development... - Kinetic characterization of Synechocystis sp. PCC6803 1-deoxy-D-xylulose 5-phosphate reductoisomerase mutantsRoberta P M Fernandes
Department of Pharmaceutical Sciences, College of Pharmacy, Pharmacy Bldg. Rm. 203, Oregon State University, Corvallis, OR 97331-3507, USA
Biochim Biophys Acta 1764:223-9. 2006..Alteration of the three acidic residues had major effects on catalysis, changes to S153 and M206 had variable effects on binding and catalysis, and a H155A mutation had only minimal effects on the kinetic parameters... - AFMoC enhances predictivity of 3D QSAR: a case study with DOXP-reductoisomeraseKatrin Silber
Department of Pharmaceutical Chemistry, Philipps University, Marbacher Weg 6, 35037 Marburg, Germany
J Med Chem 48:3547-63. 2005..Using 50% tailored fields was found to permit the precise prediction of binding affinities for related ligands without losing the capability to estimate the affinities of structurally distinct inhibitors... - Crystal structure of 1-deoxy-D-xylulose-5-phosphate reductoisomerase, a crucial enzyme in the non-mevalonate pathway of isoprenoid biosynthesisKlaus Reuter
Institut fur Pharmazeutische Chemie, Philipps Universitat, Marbacher Weg 6, D 35032 Marburg, Germany
J Biol Chem 277:5378-84. 2002..A possible involvement of this loop in an induced fit during substrate binding is discussed... - Toward Mycobacterium tuberculosis DXR inhibitor design: homology modeling and molecular dynamics simulationsNidhi Singh
Department of Medicinal Chemistry, Laboratory for Applied Drug Design and Synthesis, University of Mississippi, University, MS, 38677 1848, USA
J Comput Aided Mol Des 21:511-22. 2007.... - Molecular cloning and characterization of a 1-deoxy-D-xylulose 5-phosphate reductoisomerase gene from Ginkgo bilobaYifu Gong
Plant Biotechnology Research Center, School of Agriculture and Biology, School of Life Science and Technology, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China
DNA Seq 16:111-20. 2005..biloba than that of other tissues. The cloning and characterization of GbDXR will be helpful to understand more about the role of DXR involved in the ginkgolides biosynthesis at the molecular level... - 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripeningM Rodriguez-Concepcion
Departament de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, Martí i Franquès 1 7, 08028 Barcelona, Spain
Plant J 27:213-22. 2001..Furthermore, the complete arrest of tomato seedling development using fosmidomycin confirms a key role of the MEP pathway in plant development... - Biosynthesis of inner core lipopolysaccharide in enteric bacteria identification and characterization of a conserved phosphoheptose isomeraseJ S Brooke
Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, N6A 5C1 Canada
J Biol Chem 271:3608-14. 1996..We also demonstrated that lpcA is conserved among enteric bacteria, all of which contain glyceromannoheptose in the inner core LPS, indicating that LpcA is an essential component in a conserved biosynthetic pathway of inner core LPS... - Crystallographic studies of the mechanism of xylose isomeraseG K Farber
Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139
Biochemistry 28:7289-97. 1989..In contrast, simple sugar isomerases all require a metal ion and show very low solvent exchange. These observations are rationalized on the basis of the need for stereospecific sugar binding... - In silico studies on the substrate specificity of an l-arabinose isomerase from Bacillus licheniformisPonnandy Prabhu
Department of Bioscience and Biotechnology, Konkuk University, Gwangjin gu, Seoul 143 701, Republic of Korea
Bioorg Med Chem Lett 20:4436-9. 2010..Therefore, the differences in molecular interactions and substrate orientation in the active site of l-AIs have been examined and the residue at position 346 is proposed to be responsible for the unique substrate specificity of BLAI... - Metabolic engineering by plastid transformation as a strategy to modulate isoprenoid yield in plantsTomohisa Hasunuma
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan
Methods Mol Biol 643:213-27. 2010.... - Mannose production from fructose by free and immobilized D-lyxose isomerases from Providencia stuartiiChang Su Park
Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang dong, Gwangjin gu, Seoul 143 701, South Korea
Biotechnol Lett 32:1305-9. 2010..4 and 5.1 h at 45 degrees C, respectively. The immobilized enzyme in 300 g fructose/l (replaced hourly), produced 75 g mannose/l at 35 degrees C = 25% (w/w) yield with a productivity of 75 g mannose l(-1) h(-1) after 23 cycles... - Crystal structure of 1-deoxy-d-xylulose 5-phosphate reductoisomerase from the hyperthermophile Thermotoga maritima for insights into the coordination of conformational changes and an inhibitor bindingMihoko Takenoya
Department of Biotechnology and Life Science, Graduate School of Technology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
J Struct Biol 170:532-9. 2010..Taken together, our kinetic and the crystal structures illustrate the binding mode of fosmidomycin that leads to its slow, tight binding according to the conformational changes of DXR... - Three crystal forms of the bifunctional enzyme proline utilization A (PutA) from Bradyrhizobium japonicumJonathan P Schuermann
Department of Chemistry, University of Missouri Columbia, Columbia, MO 65211, USA
Acta Crystallogr Sect F Struct Biol Cryst Commun 64:949-53. 2008..Centered monoclinic crystals were grown in ammonium sulfate, diffracted to 2.3 A resolution and had two molecules in the asymmetric unit. Removing the histidine tag was important in order to obtain the C2 crystal form... - Use of the TRP1 auxotrophic marker for gene disruption and phenotypic analysis in yeast: a note of warningAsier González
Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Barcelona, Spain
FEMS Yeast Res 8:2-5. 2008..Therefore, its use in the past should be revisited and utilization of this marker should be avoided in future analyses... - Conformational dynamics of the flexible catalytic loop in Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomeraseSarah L Williams
Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093 0365, USA
Chem Biol Drug Des 73:26-38. 2009..The substantial fluctuations observed suggest that 1-deoxy-D-xylulose 5-phosphate reductoisomerase may be a promising target for computer-aided drug discovery through the relaxed complex method... - Rational design of Bacillus stearothermophilus US100 L-arabinose isomerase: potential applications for D-tagatose productionMoez Rhimi
Laboratoire d Enzymes et de Métabolites des Procaryotes, Centre de Biotechnologie de Sfax, Sfax Tunisie, Tunisia
Biochimie 91:650-3. 2009..This double mutant displays an optimal pH in the range 6.0-7.0 and an optimal activity around 50-65 degrees C, temperatures at which the enzyme was stable without addition of metal ions... - Genomic organization and biochemistry of the ribulose monophosphate pathway and its application in biotechnologyHiroya Yurimoto
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto, 606 8502, Japan
Appl Microbiol Biotechnol 84:407-16. 2009..Heterologous expression of HPS and PHI in various organisms allows them to metabolize and detoxify formaldehyde, and we also review recent progress in such applications in biotechnology... - Allosteric regulation of glucosamine-6-phosphate deaminase (NagB) and growth of Escherichia coli on glucosamineLaura I Alvarez-Añorve
Institut de Biologie Physico Chimique, UPR9073 CNRS, 13 rue Pierre et Marie Curie, 75005 Paris, France
J Bacteriol 191:6401-7. 2009.... - Overexpression, crystallization and preliminary X-ray crystallographic analysis of D-ribose-5-phosphate isomerase from Clostridium thermocellumJunho Jung
Department of Advanced Technology Fusion, Konkuk University, Hwayang dong, Gwangjin gu, Seoul, Republic of Korea
Acta Crystallogr Sect F Struct Biol Cryst Commun 65:1141-4. 2009..9 angstrom resolution. According to Matthews coefficient calculations, the crystallographic structure consists of a dimer in the asymmetric unit, with a V(M) of 3.2 angstrom(3) Da(-1) and a solvent content of 61.7%... - The crystal structure of a novel glucosamine-6-phosphate deaminase from the hyperthermophilic archaeon Pyrococcus furiosusKyung-Jin Kim
Pohang Accelerator Laboratory, POSTECH, Pohang 790-784, Korea
Proteins 68:413-7. 2007 - Catalytic reaction mechanism of Pseudomonas stutzeri L-rhamnose isomerase deduced from X-ray structuresHiromi Yoshida
Life Science Research Center and Faculty of Medicine, Kagawa University, Japan
FEBS J 277:1045-57. 2010..The structural analysis of D327N-substrates and additional modeling revealed Asp327 to be responsible for the ring opening of furanose, and a water molecule coordinating with the metal ion to be involved in the ring opening of pyranose... - A novel surface autolysin of Listeria monocytogenes serotype 4b, IspC, contains a 23-residue N-terminal signal peptide being processed in E. coliLinru Wang
Canadian Food Inspection Agency, Animal Diseases Research Institute, 3851 Fallowfield Road, Ottawa, Ont, Canada K2H 8P9
Biochem Biophys Res Commun 354:403-8. 2007..The highly purified form of rIspC from this study, exhibiting both peptidoglycan hydrolase activity and immunogenicity as previously reported, would facilitate further biochemical, structural, and functional studies of this autolysin... - Microbial sensors for small molecules: development of a mevalonate biosensorBrian F Pfleger
Department of Chemical Engineering, University of California Berkeley, Berkeley, CA 94720 1462, USA
Metab Eng 9:30-8. 2007.... - Characterization of a mutated Geobacillus stearothermophilus L-arabinose isomerase that increases the production rate of D-tagatoseH-J Kim
Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea
J Appl Microbiol 101:213-21. 2006..SIGNIFICANCE AND IMPACT OF THE STUDY: This work contributes knowledge on the characterization of a mutated L-arabinose isomerase, and allows an increased production rate for D-tagatose from D-galactose using the mutated enzyme... - Purification capability of tobacco transformed with enzymes from a methylotrophic bacterium for formaldehydeAyako Sawada
Graduate School of Regional Economic Systems, Kanazawa Seiryo University, Ishikawa, Japan
Int J Phytoremediation 9:487-96. 2007..The results confirmed an increase of 20% in the HCHO-removal capability. The differences of the purification capabilities for toluene, xylene, and styrene were not recognized... - Isoprenoid biosynthesis via the MEP pathway. Synthesis of (3,4)-3,4-dihydroxy-5-oxohexylphosphonic acid, an isosteric analogue of 1-deoxy-D-xylulose 5-phosphate, the substrate of the 1-deoxy-D-xylulose 5-phosphate reducto-isomeraseOdile Meyer
Universite Louis Pasteur, CNRS UMR 7123, Institut Le Bel, 4 rue Blaise Pascal, 67070 Strasbourg Cedex, France
Org Biomol Chem 1:4367-72. 2003..The enzyme was, however, less efficient with the methylene phosphonate analogue than with the natural substrate... - Molecular cloning of the Escherichia coli B L-fucose-D-arabinose gene clusterE A Elsinghorst
Section of Microbiology, Cornell University, Ithaca, New York 14853
J Bacteriol 176:7223-32. 1994..D-Arabinose metabolism by E. coli B appears to be the result of acquisitive evolution, but the source of the darK gene has not been determined... - Construction of a Trp- commercial baker's yeast strain by using food-safe-grade dominant drug resistance cassettesFrancisco Estruch
, , P.O. Box 73, 46100, Valencia, Burjassot, Spain
FEMS Yeast Res 4:329-38. 2003..These data indicate that the new Trp(-) strain can be used to generate a stable recombinant yeast that fulfils all the requirements and market criteria for commercial utilisation... - A sensitive radiometric assay to measure D-xylulose kinase activityDenis Tritsch
Institut Le Bel, Université Louis Pasteur CNRS UMR 7123, 4 rue Blaise Pascal, 67070 Strasbourg Cedex, France
J Biochem Biophys Methods 58:75-83. 2004..The radiometric assay was applied to determine xylulose kinase activity in crude cell extracts from a variety of eukaryotic and prokaryotic organisms... - Contributions of XylR CcpA and cre to diauxic growth of Bacillus megaterium and to xylose isomerase expression in the presence of glucose and xyloseD Schmiedel
, , , FRG
Mol Gen Genet 250:259-66. 1996..In contrast, a strain with an inactivated cre site in xylA exhibits diauxic growth without an apparent lag phase on glucose and xylose, whereas fructose and xylose are consumed simultaneously... - Acute ingestion of a meal rich in n-3 polyunsaturated fatty acids results in rapid gastric emptying in humansM Denise Robertson
Oxford Centre for Diabetes, Endocrinology and Metabolism, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom
Am J Clin Nutr 76:232-8. 2002..The effects on atherosclerosis may be partly mediated by the observed reduction in fasting and postprandial triacylglycerol concentrations after both acute and chronic n-3 PUFA ingestion... - Selective fitness of four episomal shuttle-vectors carrying HIS3, LEU2, TRP1, and URA3 selectable markers in Saccharomyces cerevisiaeSimone Ugolini
Microbiology Group, International Centre for Genetic Engineering and Biotechnology, Area Science Park, Padriciano 99, I-34012 Trieste, Italy
Plasmid 47:94-107. 2002..A potential correlation of the energy cost of plasmid maintenance with the secondary DNA structure and the level of expression of the selective markers is also investigated... - Substrate-dependent chemoselective aldose-aldose and aldose-ketose isomerizations of carbohydrates promoted by a combination of calcium ion and monoaminesT Tanase
Department of Chemistry, Faculty of Science, Nara Women s University, Kitauoya higashi machi, 630 8285, Nara, Japan
Carbohydr Res 333:303-12. 2001..These features are of potential interest in relevance to biomimic sugar transformations by metal ions... - Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene productionS W Kim
Marine Bioproducts Engineering Center, Department of Chemical Engineering, University of California, Berkeley, CA 94720 1462, USA
Biotechnol Bioeng 72:408-15. 2001..A comparison of the three E. coli strains transformed with the arabinose-inducible dxs on a medium-copy plasmid revealed that lycopene production was highest in XL1-Blue... - Cloning of the histidine biosynthetic genes of Corynebacterium glutamicum: organization and sequencing analysis of the hisA, impA, and hisF gene clusterS I Jung
Department of Biological Science, Sookmyung Women s University, Seoul, Korea
Biochem Biophys Res Commun 247:741-5. 1998..coli. A high similarity was observed in comparison of nucleotide sequences of each protein between C. glutamicum and other species, as well as those between hisA and hisF genes of C. glutamicum... - Biosynthesis of terpenoids: 1-deoxy-D-xylulose-5-phosphate reductoisomerase from Escherichia coli is a class B dehydrogenaseT Radykewicz
Lehrstuhl fur Organische Chemie und Biochemie, Technische Universitat Munchen, Lichtenbergstr 4, D 85747, Garching, Germany
FEBS Lett 465:157-60. 2000..Stereospecific transfer of H(Si) from C-4 of the cofactor identifies the Dxr protein of E. coli as a class B dehydrogenase...
Research Grants
- Biosynthesis of Microbial PolyketidesDavid E Cane; Fiscal Year: 2010..Finally, the proposed studies will lead to fundamental new biological insights into the central issue of modern molecular biochemistry, the relationship between protein sequence, structure, and function. ..
- GENETIC ANALYSIS OF ENZYME PROCESSING AND LOCALIZATIONElizabeth Jones; Fiscal Year: 2002..Methods will include targeted mutagenesis, suppressor selection and analysis, subcellular fractionation, biochemistry, and immunofluorescence. ..
- BIOSYNTHESIS OF MICROBIAL PRODUCTSDAVID CANE; Fiscal Year: 2000..The investigators will carry out incubations to confirm the proposed role of these two proteins and to determine the cofactor requirements and mechanism of the oxidative ring-forming reaction. ..
- Development of an Expert Crystallization Knowledge SystemEdward H Snell; Fiscal Year: 2010....
- Biosynthesis of Microbial IsoprenoidsDavid E Cane; Fiscal Year: 2010....
- Biosynthesis of Microbial PolyketidesDAVID CANE; Fiscal Year: 2009..In addition, the results obtained will provide fundamental insights into how catalysis and molecular recognition control both substrate specificity and product molecular diversity in Nature. ..
- Biosynthesis of Microbial PolyketidesDAVID CANE; Fiscal Year: 2007..In addition, the results obtained will provide fundamental insights into how catalysis and molecular recognition control both substrate specificity and product molecular diversity in Nature. ..
- STEREOCHEMICAL STUDIES OF ISOPRENOID BIOSYNTHESISDAVID CANE; Fiscal Year: 2007..abstract_text> ..
- Biosynthesis of Microbial PolyketidesDAVID CANE; Fiscal Year: 2005..coli in order to study the EpoA-catalyzed conversion of malonyl-CoA to acetyl-S-EpoA, the substrate for the NRPS module EpoB. ..
- BIOSYNTHESIS OF ANTIBIOTIC NATURAL PRODUCTSDAVID CANE; Fiscal Year: 1980....
- BIOSYNTHESIS OF ANTIBIOTIC NATURAL PRODUCTSDAVID CANE; Fiscal Year: 1992..The availability of cloned DNA will allow sequencing of the synthetase while the increased quantities of cyclase will be used for continued studies of the mechanism and stereochemistry of the cyclization reaction itself...
- BIOSYNTHESIS OF MICROBIAL NATURAL PRODUCTSDAVID CANE; Fiscal Year: 1993..The PCR products will be inserted into suitable E. coli expression vectors and the resulting overexpressed proteins will be used by Prof. Spenser and his collaborators to examine the formation of pyridoxol...
- GENETIC ANALYSIS OF ENZYME PROCESSING AND LOCALIZATIONElizabeth Jones; Fiscal Year: 1993..Methods will include targeted mutagenesis, suppressor selection and analysis, subcellular fractionation, biochemistry, and immunofluorescence...
- GENETIC ANALYSIS OF ENZYME PROCESSING AND LOCALIZATIONElizabeth Jones; Fiscal Year: 1992..The corresponding genes will be isolated and studied...
- STEREOCHEMICAL STUDIES OF ISOPRENOID BIOSYNTHESISDAVID CANE; Fiscal Year: 1993..Finally, (- )-gamma-cadinene synthetase will be isolated from A. terreus and the mechanism of cyclization of farnesyl and nerolidyl pyrophosphate to a cis, trans-germacradienyl intermediate will be examined...