isoenzymes

Summary

Summary: One of various structurally related forms of an enzyme, each having the same mechanism but with differing chemical, physical, or immunological characteristics.

Top Publications

  1. pmc ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome
    Christophe Ginestier
    Department of Internal Medicine, Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA
    Cell Stem Cell 1:555-67. 2007
  2. pmc Aldehyde dehydrogenase 1-positive cancer stem cells mediate metastasis and poor clinical outcome in inflammatory breast cancer
    Emmanuelle Charafe-Jauffret
    Department of Molecular Oncology, Institut Paoli Calmettes UMR891 Institut National de la Sante et de la Recherche Medicale, Universite de la Mediterranee, Marseille Cancer Research Center, Marseille, France
    Clin Cancer Res 16:45-55. 2010
  3. ncbi PKC-theta is required for TCR-induced NF-kappaB activation in mature but not immature T lymphocytes
    Z Sun
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York 10016, USA
    Nature 404:402-7. 2000
  4. ncbi The role of cyclooxygenases in inflammation, cancer, and development
    C S Williams
    Department of Medicine, The Vanderbilt Cancer Center, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2279, USA
    Oncogene 18:7908-16. 1999
  5. pmc Nitric oxide synthases: structure, function and inhibition
    W K Alderton
    In Vitro Pharmacology Department, GlaxoSmithKline Research and Development, Medicines Research Centre, Gunnels Wood Road, Stevenage SG1 2NY, UK
    Biochem J 357:593-615. 2001
  6. pmc Inhibition of lactate dehydrogenase A induces oxidative stress and inhibits tumor progression
    Anne Le
    Division of Hematology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:2037-42. 2010
  7. pmc The Akt kinases: isoform specificity in metabolism and cancer
    Eva Gonzalez
    Department of Biochemistry, Weill Cornell Medical College, New York, NY 10065, USA
    Cell Cycle 8:2502-8. 2009
  8. ncbi Aldehyde dehydrogenase: its role as a cancer stem cell marker comes down to the specific isoform
    Paola Marcato
    Departments of Microbiology and Immunology, Faculty of Medicine, Dalhousie University, Halifax, Novia Scotia, Canada
    Cell Cycle 10:1378-84. 2011
  9. ncbi Central role for G protein-coupled phosphoinositide 3-kinase gamma in inflammation
    E Hirsch
    Department of Genetics, Biology and Biochemistry, University of Torino, Turin, Italy
    Science 287:1049-53. 2000
  10. pmc Distinct expression levels and patterns of stem cell marker, aldehyde dehydrogenase isoform 1 (ALDH1), in human epithelial cancers
    Shan Deng
    Center for Research on Early Detection and Cure of Ovarian Cancer, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 5:e10277. 2010

Detail Information

Publications352 found, 100 shown here

  1. pmc ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome
    Christophe Ginestier
    Department of Internal Medicine, Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA
    Cell Stem Cell 1:555-67. 2007
    ..These findings offer an important new tool for the study of normal and malignant breast stem cells and facilitate the clinical application of stem cell concepts...
  2. pmc Aldehyde dehydrogenase 1-positive cancer stem cells mediate metastasis and poor clinical outcome in inflammatory breast cancer
    Emmanuelle Charafe-Jauffret
    Department of Molecular Oncology, Institut Paoli Calmettes UMR891 Institut National de la Sante et de la Recherche Medicale, Universite de la Mediterranee, Marseille Cancer Research Center, Marseille, France
    Clin Cancer Res 16:45-55. 2010
    ..To examine the role of cancer stem cells (CSC) in mediating metastasis in inflammatory breast cancer (IBC) and the association of these cells with patient outcome in this aggressive type of breast cancer...
  3. ncbi PKC-theta is required for TCR-induced NF-kappaB activation in mature but not immature T lymphocytes
    Z Sun
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York 10016, USA
    Nature 404:402-7. 2000
    ..signal transduction resulting in cytokine gene expression requires the activation of one or more of the multiple isoenzymes of serine/threonine-specific protein kinase C (PKC)...
  4. ncbi The role of cyclooxygenases in inflammation, cancer, and development
    C S Williams
    Department of Medicine, The Vanderbilt Cancer Center, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2279, USA
    Oncogene 18:7908-16. 1999
    ..The in vivo mechanism by which COX-2 affects tumor growth has not been determined. It is possible that both tumor and stromally derived COX-2 could influence tumor angiogenesis and/ or immune function...
  5. pmc Nitric oxide synthases: structure, function and inhibition
    W K Alderton
    In Vitro Pharmacology Department, GlaxoSmithKline Research and Development, Medicines Research Centre, Gunnels Wood Road, Stevenage SG1 2NY, UK
    Biochem J 357:593-615. 2001
    ..Highly selective inhibitors of iNOS versus eNOS and neuronal NOS have been identified and some of these have potential in the treatment of a range of inflammatory and other conditions in which iNOS has been implicated...
  6. pmc Inhibition of lactate dehydrogenase A induces oxidative stress and inhibits tumor progression
    Anne Le
    Division of Hematology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:2037-42. 2010
    ....
  7. pmc The Akt kinases: isoform specificity in metabolism and cancer
    Eva Gonzalez
    Department of Biochemistry, Weill Cornell Medical College, New York, NY 10065, USA
    Cell Cycle 8:2502-8. 2009
    ..Here, we summarize recent advances in understanding the roles of Akt isoforms in the regulation of metabolism and cancer, and possible mechanisms contributing to Akt isoform functional specificity...
  8. ncbi Aldehyde dehydrogenase: its role as a cancer stem cell marker comes down to the specific isoform
    Paola Marcato
    Departments of Microbiology and Immunology, Faculty of Medicine, Dalhousie University, Halifax, Novia Scotia, Canada
    Cell Cycle 10:1378-84. 2011
    ..Herein we review the suggested roles of ALDH in CSC biology and the immunohistological studies testing the potential application of ALDH isoforms as novel cancer prognostic indicators...
  9. ncbi Central role for G protein-coupled phosphoinositide 3-kinase gamma in inflammation
    E Hirsch
    Department of Genetics, Biology and Biochemistry, University of Torino, Turin, Italy
    Science 287:1049-53. 2000
    ..These results demonstrate that PI3Kgamma is a crucial signaling molecule required for macrophage accumulation in inflammation...
  10. pmc Distinct expression levels and patterns of stem cell marker, aldehyde dehydrogenase isoform 1 (ALDH1), in human epithelial cancers
    Shan Deng
    Center for Research on Early Detection and Cure of Ovarian Cancer, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 5:e10277. 2010
    ..As a novel cancer stem cell marker, ALDH1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer...
  11. pmc Aldehyde dehydrogenase 1 is a marker for normal and malignant human colonic stem cells (SC) and tracks SC overpopulation during colon tumorigenesis
    Emina H Huang
    Department of Surgery, University of Florida, Gainesville, Florida, USA
    Cancer Res 69:3382-9. 2009
    ..These findings also support our original hypothesis, derived previously from mathematical modeling of crypt dynamics, that progressive colonic SC overpopulation occurs during colon tumorigenesis and drives CRC development...
  12. pmc Protein kinase C theta affects Ca2+ mobilization and NFAT cell activation in primary mouse T cells
    Christa Pfeifhofer
    Institute of Medical Biology and Human Genetics, University of Innsbruck, Schoepfstrasse 41, A 6020 Innsbruck, Austria
    J Exp Med 197:1525-35. 2003
    ..Our finding suggests that PKCtheta plays a critical and nonredundant role in T cell receptor-induced NFAT activation...
  13. ncbi The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42
    G Joberty
    Markey Center for Cell Signaling, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908 0577, USA
    Nat Cell Biol 2:531-9. 2000
    ..This assembly is implicated in the formation of normal tight junctions at epithelial cell-cell contacts. Thus, Par6 is a key adaptor that links Cdc42 and atypical PKCs to Par3...
  14. doi Aldehyde dehydrogenase discriminates the CD133 liver cancer stem cell populations
    Stephanie Ma
    Department of Clinical Oncology, The University of Hong Kong, Pokfulam, Hong Kong, China
    Mol Cancer Res 6:1146-53. 2008
    ..ALDH, expressed along CD133, can more specifically characterize the tumorigenic liver CSC population...
  15. ncbi Structure, function, and control of phosphoinositide-specific phospholipase C
    M J Rebecchi
    Departments of Anesthesiology and Physiology and Biophysics, School of Medicine, State University of New York, Stony Brook, New York 11794, USA
    Physiol Rev 80:1291-335. 2000
    ..In this review we consider each subtype of PLC in organisms ranging from yeast to mammals and discuss their molecular regulation and biological function...
  16. ncbi Isozymes of mammalian hexokinase: structure, subcellular localization and metabolic function
    John E Wilson
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA
    J Exp Biol 206:2049-57. 2003
    ..Immunolocalization studies have indicated that, in many cell types, the Type III has a perinuclear localization, the possible metabolic consequences of which remain unclear...
  17. doi Association of breast cancer stem cells identified by aldehyde dehydrogenase 1 expression with resistance to sequential Paclitaxel and epirubicin-based chemotherapy for breast cancers
    Tomonori Tanei
    Department of Breast and Endocrine Surgery, Osaka University Graduate School of Medicine, Osaka, Japan
    Clin Cancer Res 15:4234-41. 2009
    ..The aim of this study was to investigate whether cancer stem cells were clinically significant for resistance to chemotherapy in human breast cancers...
  18. pmc Non-P450 aldehyde oxidizing enzymes: the aldehyde dehydrogenase superfamily
    Satori A Marchitti
    University of Colorado Health Sciences Center, Molecular Toxicology and Environmental Health Sciences Program, Department of Pharmaceutical Sciences, 4200 East Ninth Avenue, C238, Denver, Colorado 80262, USA
    Expert Opin Drug Metab Toxicol 4:697-720. 2008
    ..While several non-P450 enzyme systems participate in their metabolism, one of the most important is the aldehyde dehydrogenase (ALDH) superfamily, composed of NAD(P)+-dependent enzymes that catalyze aldehyde oxidation...
  19. pmc Regulation of OPA1 processing and mitochondrial fusion by m-AAA protease isoenzymes and OMA1
    Sarah Ehses
    Institute for Genetics, University of Cologne, Germany
    J Cell Biol 187:1023-36. 2009
    ..we demonstrate that two classes of metallopeptidases regulate OPA1 cleavage in the mitochondrial inner membrane: isoenzymes of the adenosine triphosphate (ATP)-dependent matrix AAA (ATPase associated with diverse cellular activities [m-..
  20. doi Circulating tumor cells with a putative stem cell phenotype in peripheral blood of patients with breast cancer
    Panayiotis A Theodoropoulos
    Department of Biochemistry, School of Medicine, University of Crete, Heraklion, Greece
    Cancer Lett 288:99-106. 2010
    ..7% of the CTCs analyzed in seven patients, were ALDH1(high)/CD24(-/low). In conclusion, we report the existence of a subpopulation of CTCs with putative stem cell progenitor phenotypes in patients with metastatic breast cancer...
  21. ncbi Essential role of phosphoinositide 3-kinase delta in neutrophil directional movement
    Chanchal Sadhu
    ICOS Corporation, Bothell, WA 98021, USA
    J Immunol 170:2647-54. 2003
    ..These results demonstrate that PI3K delta can play a selective role in the amplification of PIP3 levels that lead to neutrophil polarization and directional migration...
  22. pmc A pharmacological map of the PI3-K family defines a role for p110alpha in insulin signaling
    Zachary A Knight
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
    Cell 125:733-47. 2006
    ..These results illustrate systematic target validation using a matrix of inhibitors that span a protein family...
  23. ncbi Phospholipase C is a key enzyme regulating intracellular calcium and modulating the phosphoinositide balance
    Kiyoko Fukami
    Laboratory of the Genome and Biosignals, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo, Japan
    Prog Lipid Res 49:429-37. 2010
    ..In this review, we focus on the roles of PLC as a calcium-regulating enzyme and as a modulator of the phosphoinositide balance...
  24. pmc Expression of aldehyde dehydrogenase 1 (ALDH1) is associated with basal-like markers and features of aggressive tumours in African breast cancer
    H Nalwoga
    Section for Pathology, The Gade Institute, University of Bergen, Haukeland University Hospital, N 5021, Bergen, Norway
    Br J Cancer 102:369-75. 2010
    ..The aim of this study was to explore the expression of these proteins in breast cancers from an African population and their associations with the basal-like phenotype (BLP) and other molecular characteristics...
  25. doi Multiple Rubisco forms in proteobacteria: their functional significance in relation to CO2 acquisition by the CBB cycle
    Murray Ronald Badger
    Molecular Plant Physiology Group, Research School of Biological Sciences, The Australian National University, Canberra, ACT, Australia
    J Exp Bot 59:1525-41. 2008
    ....
  26. ncbi Attenuation of LDH-A expression uncovers a link between glycolysis, mitochondrial physiology, and tumor maintenance
    Valeria R Fantin
    Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA
    Cancer Cell 9:425-34. 2006
    ..These results demonstrate that LDH-A plays a key role in tumor maintenance...
  27. doi PKC inhibitors: potential in T cell-dependent immune diseases
    Gottfried Baier
    Department for Medical Genetics, Molecular and Clinical Pharmacology, Innsbruck Medical University, Innsbruck, Austria
    Curr Opin Cell Biol 21:262-7. 2009
    ..Here we review the topic of PKC pathway activity in the regulation of T lymphocytes both in the cytokine response and adhesive capacity, and review recent results with PKC inhibitors in vitro and in vivo...
  28. ncbi Protein kinase Ctheta: a new essential superstar on the T-cell stage
    A Altman
    Division of Cell Biology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
    Immunol Today 21:567-73. 2000
    ..Among these, the nuclear factor-kappaB (NF-kappaB) signaling cascade appears to be the most critical target of PKCtheta in the T-cell receptor/CD28 costimulatory pathway that leads to T-cell activation...
  29. pmc The 4-coumarate:CoA ligase gene family in Arabidopsis thaliana comprises one rare, sinapate-activating and three commonly occurring isoenzymes
    Björn Hamberger
    Max Planck Institute for Plant Breeding Research, Carl von Linne Weg 10, 50829 Cologne, Germany
    Proc Natl Acad Sci U S A 101:2209-14. 2004
    ..Various characteristics shared by all known plant 4CL genes, as well as by the encoded proteins, define and delimit the At4CL gene family and distinguish it from the closely related family of "At4CL-like" genes...
  30. pmc Cyclooxygenase-2 regulates mesenchymal cell differentiation into the osteoblast lineage and is critically involved in bone repair
    Xinping Zhang
    The Center for Musculoskeletal Research, University of Rochester Medical Center, New York 14642, USA
    J Clin Invest 109:1405-15. 2002
    ..These results provide a model whereby COX-2 regulates the induction of cbfa1 and osterix to mediate normal skeletal repair...
  31. pmc Heterogeneity for stem cell-related markers according to tumor subtype and histologic stage in breast cancer
    So Yeon Park
    Department of Pathology, Seoul National University College of Medicine, Seoul, Korea
    Clin Cancer Res 16:876-87. 2010
    ..To evaluate the expression of stem cell-related markers at the cellular level in human breast tumors of different subtypes and histologic stage...
  32. ncbi Mechanism of diacylglycerol-induced membrane targeting and activation of protein kinase Cdelta
    Robert V Stahelin
    Department of Chemistry, University of Illinois, Chicago, Illinois 60607, USA
    J Biol Chem 279:29501-12. 2004
    ..Collectively, these results provide new insight into the differential roles of C1 domains in the DAG-induced membrane activation of PKCdelta and the origin of its specific subcellular localization in response to DAG...
  33. ncbi Cyclooxygenase-2 inhibition by celecoxib reduces proliferation and induces apoptosis in angiogenic endothelial cells in vivo
    Kathleen M Leahy
    Pharmacia, Mail Zone AA4C, 700 Chesterfield Parkway, Chesterfield, Missouri 63017, USA
    Cancer Res 62:625-31. 2002
    ..2-3.0-fold) in response to celecoxib. Thus, the antitumor activity of celecoxib may be attributable, at least in part, to a direct effect on host stromal elements, such as the angiogenic vasculature...
  34. ncbi Expression of bone resorption genes in osteoarthritis and in osteoporosis
    Darja Bitenc Logar
    Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, SI 1000, Ljubljana, Slovenia
    J Bone Miner Metab 25:219-25. 2007
    ....
  35. ncbi Protein kinase C(theta) in T cell activation
    Noah Isakov
    Department of Microbiology and Immunology, Faculty of Health Sciences, and the Cancer Research Center, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Annu Rev Immunol 20:761-94. 2002
    ..The selective expression of PKC theta in T cells and its essential role in mature T cell activation establish it as an attractive drug target for immunosuppression in transplantation and autoimmune diseases...
  36. pmc Stem cell and epithelial-mesenchymal transition markers are frequently overexpressed in circulating tumor cells of metastatic breast cancer patients
    Bahriye Aktas
    Department of Gynecology and Obstetrics, University Hospital Essen, University of Duisburg Essen, Hufelandstrasse 55, 45122 Essen, Germany
    Breast Cancer Res 11:R46. 2009
    ....
  37. pmc CD3/CD28 costimulation-induced NF-kappaB activation is mediated by recruitment of protein kinase C-theta, Bcl10, and IkappaB kinase beta to the immunological synapse through CARMA1
    Donghai Wang
    Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, University at Buffalo, SUNY, Buffalo, New York 14214, USA
    Mol Cell Biol 24:164-71. 2004
    ..Together, these results indicate that CARMA1 mediates CD3/CD28 costimulation-induced NF-kappaB activation by recruiting downstream signaling components into the immunological synapse...
  38. ncbi The role of protein kinase C activation and the vascular complications of diabetes
    Net Das Evcimen
    Ankara University, Pharmacy Faculty, Biochemistry Department, 06100 Ankara, Turkey
    Pharmacol Res 55:498-510. 2007
    ..PKC isoform selective inhibitors are likely new therapeutics, which can delay the onset or stop the progression of diabetic vascular disease with very little side effects...
  39. pmc JNK-mediated induction of cyclooxygenase 2 is required for neurodegeneration in a mouse model of Parkinson's disease
    Stephane Hunot
    Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 101:665-70. 2004
    ..Our data revealed that JNK2- and JNK3-induced COX-2 may be a principle pathway responsible for neurodegeneration in PD...
  40. ncbi Signalling by PI3K isoforms: insights from gene-targeted mice
    Bart Vanhaesebroeck
    Ludwig Institute for Cancer Research, 91 Riding House Street, London W1W 7BS, UK
    Trends Biochem Sci 30:194-204. 2005
    ..Targeting strategies that take into account the complex interplay between members of the PI3K family will be crucial to gain a full understanding of the physiological roles of the isoforms of PI3K...
  41. pmc Increased anxiety and altered responses to anxiolytics in mice deficient in the 65-kDa isoform of glutamic acid decarboxylase
    S F Kash
    Departments of Medicine and Microbiology Immunology, and Hormone Research Institute, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 96:1698-703. 1999
    ..We propose that stress-induced GABA release is impaired in GAD65-deficient mice, resulting in increased anxiety-like responses and a diminished response to the acute effects of drugs that facilitate the actions of released GABA...
  42. ncbi Physiological roles of PKB/Akt isoforms in development and disease
    B Dummler
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH 4058 Basel, Switzerland
    Biochem Soc Trans 35:231-5. 2007
    ....
  43. ncbi TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue
    D A Kujubu
    Department of Biological Chemistry, UCLA School of Medicine 90024
    J Biol Chem 266:12866-72. 1991
    ..The expression of the TIS10 gene appears to be highly cell type-restricted in cultured cell lines; of 12 cell lines tested under superinducing conditions, only the rodent embryonic Swiss 3T3 and Rat1 cell lines expressed TIS10 gene...
  44. ncbi LDH-A silencing suppresses breast cancer tumorigenicity through induction of oxidative stress mediated mitochondrial pathway apoptosis
    Zhi Yu Wang
    School of Chinese Medicine, The University of Hong Kong, Estates Building, 10 Sassoon Road, Hong Kong, China
    Breast Cancer Res Treat 131:791-800. 2012
    ..Our results suggested that LDH-A inhibition might offer a promising therapeutic strategy for breast cancer...
  45. pmc Nonredundant functions for Akt isoforms in astrocyte growth and gliomagenesis in an orthotopic transplantation model
    Raelene Endersby
    Department of Developmental Neurobiology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Cancer Res 71:4106-16. 2011
    ..Taken together, these results indicate context-dependent roles for individual Akt isoforms and suggest that there may be heterogeneous tumor response to isoform-specific inhibitors...
  46. ncbi Biochemistry and physiology of cyclic nucleotide phosphodiesterases: essential components in cyclic nucleotide signaling
    Marco Conti
    Division of Reproductive Biology, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California 943095 5317, USA
    Annu Rev Biochem 76:481-511. 2007
    ....
  47. ncbi Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6
    H Enslen
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester 01605, USA
    J Biol Chem 273:1741-8. 1998
    ..The MKK3 and MKK6 signal transduction pathways are therefore coupled to distinct, but overlapping, groups of p38 MAP kinases...
  48. pmc PKC-θ is a drug target for prevention of T cell-mediated autoimmunity and allograft rejection
    Myung Ja Kwon
    Beckman Research Institute of the City of Hope, Duarte, CA 91010, Los Angeles, USA
    Endocr Metab Immune Disord Drug Targets 10:367-72. 2010
    ..Since these effector T helper cells are responsible for mediating autoimmunity, selective inhibition of PKC-θ is considered a treatment for prevention of autoimmune diseases and allograft rejection...
  49. ncbi Regulation of protein kinase C
    A C Newton
    Department of Pharmacology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0640, USA
    Curr Opin Cell Biol 9:161-7. 1997
    ....
  50. pmc Protein kinase C betaII and PKCiota/lambda: collaborating partners in colon cancer promotion and progression
    Nicole R Murray
    Department of Cancer Biology, Mayo Clinic College of Medicine, Jacksonville, Florida 32224, USA
    Cancer Res 69:656-62. 2009
    ..Thus, PKCbetaII and PKCiota, whose expression is elevated in both rodent and human colon tumors, collaborate to drive colon tumor formation and progression, respectively...
  51. ncbi Molecular cloning and characterization of PKC iota, an atypical isoform of protein kinase C derived from insulin-secreting cells
    L A Selbie
    Garvan Institute of Medical Research, St Vincent s Hospital, Sydney, Australia
    J Biol Chem 268:24296-302. 1993
    ..As such, PKC iota is unlikely to be activated by the diacylglycerol that is derived from phosphoinositide hydrolysis, but might be a target for novel lipid activators that are elevated during nutrient-stimulated insulin secretion...
  52. pmc Protein kinase C theta is critical for the development of in vivo T helper (Th)2 cell but not Th1 cell responses
    Benjamin J Marsland
    Molecular Biomedicine, Swiss Federal Institute of Technology, Wagistr 27, CH8952 Zurich Schlieren, Switzerland
    J Exp Med 200:181-9. 2004
    ..Taken together, our results reveal a central role for PKC-theta signaling during Th2 responses...
  53. ncbi A novel small-molecule inhibitor of protein kinase Ciota blocks transformed growth of non-small-cell lung cancer cells
    Melody Stallings-Mann
    Department of Cancer Biology, Comprehensive Cancer Center, Mayo Clinic College of Medicine, 4500 San Pablo Road, Jacksonville, FL 32224, USA
    Cancer Res 66:1767-74. 2006
    ..ATG and ATM will be useful reagents for studying PKCiota function in transformation and represent promising new agents for the clinical treatment of NSCLC...
  54. ncbi Hypoxia induces cyclooxygenase-2 via the NF-kappaB p65 transcription factor in human vascular endothelial cells
    J F Schmedtje
    Sealy Center for Molecular Cardiology, Department of Medicine, The University of Texas Medical Branch, Galveston 77555 1064, USA
    J Biol Chem 272:601-8. 1997
    ..Thus, the intracellular signaling mechanism that leads to induction of COX-2 by hypoxia includes binding of p65 to the relatively 3' NF-kappaB consensus element in the COX-2 upstream promoter region in human vascular endothelial cells...
  55. ncbi Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation
    Daniel J Weisenberger
    Urologic Cancer Research Laboratory, Department of Biochemistry and Molecular Biology, University of Southern California Norris Comprehensive Cancer Center, Keck School of Medicine, 1441 Eastlake Avenue, Los Angeles, CA 90089 9181, USA
    Mol Cancer Res 2:62-72. 2004
    ..Methylation analyses of immunodeficiency, chromosomal instabilities, and facial abnormalities cells revealed that an Alu repeat sequence was highly methylated, suggesting that Alu sequences are not DNMT3b targets...
  56. ncbi Keynote review: phosphodiesterase-4 as a therapeutic target
    Miles D Houslay
    Division of Biochemistry and Molecular Biology, IBLS, Wolfson Link Building, University of Glasgow, University Avenue, Glasgow, G12 8QQ, Scotland, UK
    Drug Discov Today 10:1503-19. 2005
    ..Here, we delineate the range of PDE4 isoforms, their role in signaling, their structural biology and related preclinical and clinical pharmacology...
  57. ncbi Inhibition of the cyclooxygenase isoenzymes COX-1 and COX-2 provide neuroprotection in the MPTP-mouse model of Parkinson's disease
    P Teismann
    Institute of Pharmacology and Toxicology, Faculty of Pharmacy, University of Marburg, Germany
    Synapse 39:167-74. 2001
    To study the possible role of the isoenzymes of cyclooxygenase COX-1 and COX-2 in the MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) mouse model of Parkinson's disease we used acetylsalicylic acid, a COX-1/COX-2 inhibitor, in ..
  58. pmc Treatment of Fabry disease: outcome of a comparative trial with agalsidase alfa or beta at a dose of 0.2 mg/kg
    Anouk C Vedder
    Department of Internal Medicine Endocrinology and Metabolism, Academic Medical Center, Amsterdam, Netherlands Department of Medical Biochenmistry, Academic Medical Center University of Amsterdam, Amsterdam, Netherlands
    PLoS ONE 2:e598. 2007
    ..We compared the efficacy of and tolerability towards the two agalsidase preparations administered at identical protein dose in a randomized controlled open label trial...
  59. doi 5α-reductase type 3 expression in human benign and malignant tissues: a comparative analysis during prostate cancer progression
    Alejandro Godoy
    Department of Urology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA
    Prostate 71:1033-46. 2011
    ..A third isozyme of human 5α-steroid reductase, 5α-reductase-3, was identified in prostate tissue at the mRNA level. However, the levels of 5α-reductase-3 protein expression and its cellular localization in human tissues remain unknown...
  60. ncbi Crucial importance of PKC-beta(I) in LFA-1-mediated locomotion of activated T cells
    Y Volkov
    Dublin Molecular Medicine Centre, Trinity College, Dublin 8, Ireland
    Nat Immunol 2:508-14. 2001
    ..These observations may have physiological equivalents at advanced (post-integrin activation) stages of lymphocyte extravasation...
  61. pmc LDH-A inhibition, a therapeutic strategy for treatment of hereditary leiomyomatosis and renal cell cancer
    Han Xie
    Department of Medicine, Division of Interdisciplinary Medicine and Biotechnology, Beth Israel Deaconess Medical Center, Harvard Medical School, and Department of Pathology, Brigham and Women s Hospital, Boston, MA 02215, USA
    Mol Cancer Ther 8:626-35. 2009
    ....
  62. ncbi Physical and functional interactions between the human DNMT3L protein and members of the de novo methyltransferase family
    Zhao Xia Chen
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    J Cell Biochem 95:902-17. 2005
    ..This article contains Supplementary Material available at http://www.mrw.interscience.wiley.com/suppmat/0730-2312/suppmat/2005/95/chen.html...
  63. doi Enzyme replacement therapy with agalsidase alfa in patients with Fabry's disease: an analysis of registry data
    A Mehta
    University College London, London, UK
    Lancet 374:1986-96. 2009
    ..We analysed 5-year treatment with agalsidase alfa enzyme replacement therapy in patients with Fabry's disease who were enrolled in the Fabry Outcome Survey observational database (FOS)...
  64. pmc Agalsidase alfa and kidney dysfunction in Fabry disease
    Michael West
    Division of Nephrology, Department of Medicine, Dalhousie University, 5090 ACC QE II Health Sciences Centre, 5820 University Avenue, Halifax, NS, Canada B3H 1V8
    J Am Soc Nephrol 20:1132-9. 2009
    ..These data suggest that agalsidase alfa may stabilize kidney function in these patients...
  65. ncbi Sustained, long-term renal stabilization after 54 months of agalsidase beta therapy in patients with Fabry disease
    Dominique P Germain
    Assistance Publique Hopitaux de Paris, Paris, France
    J Am Soc Nephrol 18:1547-57. 2007
    ..Long-term agalsidase beta therapy stabilizes renal function in patients without renal involvement at baseline, maintains reduction of plasma GL-3, and sustains GL-3 clearance in capillary endothelial cells and multiple renal cell types...
  66. ncbi Specificity in the cAMP/PKA signaling pathway. Differential expression,regulation, and subcellular localization of subunits of PKA
    B S Skalhegg
    Institute for Nutrition Reserarch, University of Oslo, Norway
    Front Biosci 5:D678-93. 2000
    ..Here we will describe features of this signaling pathway that may contribute to explain how differential effects of cAMP may be contributed to features of the PKA signaling pathway...
  67. doi Stem cell marker aldehyde dehydrogenase 1-positive breast cancers are characterized by negative estrogen receptor, positive human epidermal growth factor receptor type 2, and high Ki67 expression
    Koji Morimoto
    Department of Breast and Endocrine Surgery, Osaka University Graduate School of Medicine, Suita City, Osaka, Japan
    Cancer Sci 100:1062-8. 2009
    ..Breast cancers with ALDH1+ cancer stem cells posses biologically aggressive phenotypes that tend to have a poor prognosis, and ALDH1+ cancer stem cells are characterized by ER-, Ki67-, and HER2+...
  68. pmc Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes
    Robert V Farese
    James A Haley Veterans Medical Center, Tampa, Florida 33612, USA
    J Clin Invest 117:2289-301. 2007
    ..These findings are particularly relevant because humans who have obesity, impaired glucose tolerance, and T2DM reportedly have defective activation and/or diminished levels of muscle aPKC...
  69. pmc Epilepsy in mice deficient in the 65-kDa isoform of glutamic acid decarboxylase
    S F Kash
    Department of Medicine, School of Medicine, University of California at San Francisco, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 94:14060-5. 1997
    ....
  70. ncbi Sequential activation of class IB and class IA PI3K is important for the primed respiratory burst of human but not murine neutrophils
    Alison M Condliffe
    The Inositide Laboratory, Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, UK
    Blood 106:1432-40. 2005
    ..These results start to define which PI3K isoforms are responsible for modulating neutrophil responsiveness to infection and inflammation...
  71. pmc ALDH activity selectively defines an enhanced tumor-initiating cell population relative to CD133 expression in human pancreatic adenocarcinoma
    Michael P Kim
    Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America
    PLoS ONE 6:e20636. 2011
    ..Understanding which pancreatic cancer cell populations possess tumor-initiating capabilities is critical to characterizing and understanding the biology of pancreatic CSCs towards therapeutic ends...
  72. ncbi CARMA1 is a critical lipid raft-associated regulator of TCR-induced NF-kappa B activation
    Olivier Gaide
    Institute of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Nat Immunol 3:836-43. 2002
    ..Together, our data show that CARMA1 is a critical lipid raft-associated regulator of TCR-induced NF-kappa B activation and CD28 costimulation-dependent Jnk activation...
  73. pmc Protein kinase Cι expression and oncogenic signaling mechanisms in cancer
    Nicole R Murray
    Department of Cancer Biology, Mayo Clinic Comprehensive Cancer Center, Jacksonville, Florida 32224, USA
    J Cell Physiol 226:879-87. 2011
    ....
  74. doi Osteoblastogenesis and osteoprotection enhanced by flavonolignan silibinin in osteoblasts and osteoclasts
    Jung Lye Kim
    Department of Food and Nutrition, Hallym University, Chuncheon, Korea
    J Cell Biochem 113:247-59. 2012
    ..These results demonstrate that silibinin was a potential therapeutic agent promoting bone-forming osteoblastogenesis and encumbering osteoclastic bone resorption...
  75. doi Selective PDE inhibitors as novel treatments for respiratory diseases
    Clive P Page
    Sackler Institute of Pulmonary Pharmacology, Institute of Pharmaceutical Science, Franklin Wilkins Building, King s College London, London SE1 9NH, UK
    Curr Opin Pharmacol 12:275-86. 2012
    ....
  76. ncbi Cyclooxygenase-1 is overexpressed and promotes angiogenic growth factor production in ovarian cancer
    Rajnish A Gupta
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Cancer Res 63:906-11. 2003
    ....
  77. pmc Ablation of sphingosine kinase-2 inhibits tumor cell proliferation and migration
    Peng Gao
    Department of Pharmaceutical and Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Cancer Res 9:1509-19. 2011
    ..between proapoptotic ceramides and mitogenic sphingosine-1-phosphate (S1P); however, the functions of the two isoenzymes (SK1 and SK2) in tumor cells are not well defined...
  78. pmc Phospholipase C isozymes are deregulated in colorectal cancer--insights gained from gene set enrichment analysis of the transcriptome
    Stine A Danielsen
    Department of Cancer Prevention, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway
    PLoS ONE 6:e24419. 2011
    ..In conclusion, we have identified significantly deregulated pathways in CRC, and validated repression of PLCD1 and PLCE1 expression. This illustrates that the GSEA approach may guide discovery of novel biomarkers in cancer...
  79. ncbi Glucose 1-phosphate is efficiently taken up by potato (Solanum tuberosum) tuber parenchyma cells and converted to reserve starch granules
    Joerg Fettke
    Institute of Biochemistry and Biology, Mass Spectrometry of Biopolymers, University of Potsdam, Karl Liebknecht Str 24 25, Building 20, D 14476 Potsdam Golm, Germany
    New Phytol 185:663-75. 2010
    ..Two distinct paths of reserve starch biosynthesis are proposed that explain, at a biochemical level, the phenotype of several transgenic plant lines...
  80. ncbi Cyclooxygenase regulates angiogenesis induced by colon cancer cells
    M Tsujii
    Department of Medicine, Vanderbilt University Medical Center, VA Medical Center, Nashville, Tennessee 37232, USA
    Cell 93:705-16. 1998
    ..Cyclooxygenase regulates colon carcinoma-induced angiogenesis by two mechanisms: COX-2 can modulate production of angiogenic factors by colon cancer cells, while COX-1 regulates angiogenesis in endothelial cells...
  81. doi Arabidopsis chloroplastic ascorbate peroxidase isoenzymes play a dual role in photoprotection and gene regulation under photooxidative stress
    Takanori Maruta
    Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara, 631 8505 Japan
    Plant Cell Physiol 51:190-200. 2010
    ..The present findings suggest that both chloroplastic APXs, but particularly tAPX, are important for photoprotection and gene regulation under photooxidative stress in Arabidopsis leaves...
  82. doi Isoform-specific roles of protein phosphatase 1 catalytic subunits in sarcoplasmic reticulum-mediated Ca(2+) cycling
    Hidekazu Aoyama
    Division of Cardiology, Department of Medicine and Clinical Science, Yamaguchi University Graduate School of Medicine, 1 1 1 Minami kogushi, Ube, Yamaguchi 755 8505, Japan
    Cardiovasc Res 89:79-88. 2011
    ..We sought to determine the functional contribution of each PP1 isoform to sarcoplasmic reticulum (SR)-mediated Ca(2+) cycling in isolated adult rat cardiomyocytes...
  83. ncbi The activity of class I, II, III, and IV alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in esophageal cancer
    Wojciech Jelski
    Department of Biochemical Diagnostics, Medical University, Waszyngtona 15 A, 15 269, Bialystok, Poland
    Dig Dis Sci 54:725-30. 2009
    ..In this study the activity of ADH isoenzymes and ALDH in esophageal cancer were compared with the activity in normal tissue.
  84. ncbi Protein kinase C (PKC) family in cancer progression
    Jussi Koivunen
    Department of Anatomy and Cell Biology, University of Oulu, P O Box 5000, 90014 Oulu, Finland
    Cancer Lett 235:1-10. 2006
    ..Potentiation of malignant phenotype may be mediated by activation of selective PKC isoenzymes or through altered isoenzyme expression profile compared to the originating tissue...
  85. ncbi A requirement for CARMA1 in TCR-induced NF-kappa B activation
    Donghai Wang
    Department of Microbiology, School of Medicine and Biomedical Sciences, University at Buffalo, SUNY, Buffalo, NY 14214, USA
    Nat Immunol 3:830-5. 2002
    ..Reconstitution of the CARMA1-deficient cells with CARMA1 fully rescued this signaling defect. Together, our results show that CARMA1 is an essential signaling component that mediates TCR-induced NF-kappa B activation...
  86. ncbi Furan-2-ylmethylene thiazolidinediones as novel, potent, and selective inhibitors of phosphoinositide 3-kinase gamma
    Vincent Pomel
    Department of Chemistry, Serono Pharmaceutical Research Institute, 14 Chemin des Aulx, CH 1228 Plan les Ouates, Geneva, Switzerland
    J Med Chem 49:3857-71. 2006
    ..Oral administration of 26 in a mouse model of acute peritonitis led to a significant reduction of leukocyte recruitment...
  87. ncbi PI 3-kinase p110beta: a new target for antithrombotic therapy
    Shaun P Jackson
    Australian Centre for Blood Diseases, Monash University, 6th Floor Burnet Building Alfred Medical Research and Education Precinct, Prahran, Victoria, Australia 3181
    Nat Med 11:507-14. 2005
    ..In vivo, these inhibitors eliminate occlusive thrombus formation but do not prolong bleeding time. These studies define PI3K p110beta as an important new target for antithrombotic therapy...
  88. pmc Atypical protein kinase C{iota} is required for bronchioalveolar stem cell expansion and lung tumorigenesis
    Roderick P Regala
    Departments of Cancer Biology and Pathology, Mayo Clinic Comprehensive Cancer Center, Jacksonville, Florida, USA
    Cancer Res 69:7603-11. 2009
    ..These data have important implications for PKCiota as a therapeutic target and for the clinical use of aurothiomalate for lung cancer treatment...
  89. ncbi Breast cancer stem cells and intrinsic subtypes: controversies rage on
    Harikrishna Nakshatri
    Department of Surgery, Indiana University School of Medicine, 1044 West Walnut Street, Indianapolis, IN 46202, USA
    Curr Stem Cell Res Ther 4:50-60. 2009
    ....
  90. ncbi Comparative genomics and proteomics of vertebrate diacylglycerol acyltransferase (DGAT), acyl CoA wax alcohol acyltransferase (AWAT) and monoacylglycerol acyltransferase (MGAT)
    Roger S Holmes
    School of Biomolecular and Physical Sciences, Griffith University, Nathan 4111 Brisbane, Queensland, Australia
    Comp Biochem Physiol Part D Genomics Proteomics 5:45-54. 2010
    ....
  91. doi Prognostic impact of ALDH1 in breast cancer: a story of stem cells and tumor microenvironment
    Erika Resetkova
    Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX 46202, USA
    Breast Cancer Res Treat 123:97-108. 2010
    ..The novel finding is that tumor microenvironment may play a significant role in determining the prognostic impact of stem/progenitor cells in human breast tumors...
  92. pmc SIRT1 and SIRT3 deacetylate homologous substrates: AceCS1,2 and HMGCS1,2
    Matthew D Hirschey
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94158, USA
    Aging (Albany NY) 3:635-42. 2011
    ..In this perspective, we take a first step by characterizing the evolutionary history of the sirtuins and these substrate families...
  93. doi The prognostic role of cancer stem cells in breast cancer: a meta-analysis of published literatures
    Liheng Zhou
    Department of Breast Surgery, Shanghai Cancer Hospital Cancer Institute, Fudan University, 399 Ling Ling Road, Shanghai 200032, China
    Breast Cancer Res Treat 122:795-801. 2010
    ..001) were significantly associated with poor overall survival (OS). The stem cell markers are prognostic factors in breast cancer. Larger clinical studies are required to further evaluate the role of these markers in clinical practice...
  94. ncbi A heterodimer of human 3'-phospho-adenosine-5'-phosphosulphate (PAPS) synthases is a new sulphate activating complex
    Daniel Grum
    Structural and Medicinal Biochemistry, Centre for Medical Biotechnology ZMB, Faculty of Biology and Geography, University of Duisburg Essen, 45117 Essen, Germany
    Biochem Biophys Res Commun 395:420-5. 2010
    ..Due to divergent catalytic efficacies of PAPSS1 and -S2, the heterodimer might be a way of regulating PAPS synthase function within mammalian cells...
  95. ncbi Function and regulation of the mitochondrial sirtuin isoform Sirt5 in Mammalia
    Melanie Gertz
    Department of Physiological Chemistry, Ruhr University Bochum, Bochum, Germany
    Biochim Biophys Acta 1804:1658-65. 2010
    ....
  96. ncbi Catalytic domain of phosphoinositide-specific phospholipase C (PLC). Mutational analysis of residues within the active site and hydrophobic ridge of plcdelta1
    M V Ellis
    Cancer Research Campaign Centre for Cell and Molecular Biology, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, United Kingdom
    J Biol Chem 273:11650-9. 1998
    ....
  97. ncbi 1.3-A resolution structure of human glutathione S-transferase with S-hexyl glutathione bound reveals possible extended ligandin binding site
    Isolde Le Trong
    Department of Biological Structure, University of Washington, Seattle Washington 98195, USA
    Proteins 48:618-27. 2002
    ..Finally, detailed comparison of the structures reported here with previously published X-ray structures reveal a possible reaction coordinate for ligand-dependent conformational changes in the active site and the C-terminus...
  98. pmc Evidence for functional redundancy of class IA PI3K isoforms in insulin signalling
    Claire Chaussade
    Maurice Wilkins Centre for Molecular Biodiscovery, Department of Molecular Medicine and Pathology, University of Auckland, Private Bag 92019, Auckland, New Zealand
    Biochem J 404:449-58. 2007
    ..Further, our results indicate that the degree of functional redundancy is linked to the relative levels of expression of each isoform in the target cells...
  99. pmc Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice
    Polina V Danshina
    Laboratories for Reproductive Biology, Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7090, USA
    Biol Reprod 82:136-45. 2010
    ..In addition to confirming the importance of the glycolytic pathway for sperm function, distinctive phenotypic characteristics of Pgk2(-/-) mice may provide further insights into the regulation of sperm metabolism...
  100. pmc The interaction of p62 with RIP links the atypical PKCs to NF-kappaB activation
    L Sanz
    Laboratorio Glaxo Wellcome CSIC de Biología Molecular y Celular, Centro de Biología Molecular Severo Ochoa Consejo Superior de Investigaciones Científicas Universidad Autónoma de Madrid, Spain
    EMBO J 18:3044-53. 1999
    ..Together, these results demonstrate that the interaction of p62 with RIP serves to link the atypical PKCs to the activation of NF-kappaB by the TNFalpha signaling pathway...
  101. ncbi A novel isoform of human cyclic 3',5'-adenosine monophosphate-dependent protein kinase, c alpha-s, localizes to sperm midpiece
    N Reinton
    Institute of Medical Biochemistry, University of Oslo, N 0317 Oslo, Norway
    Biol Reprod 63:607-11. 2000
    ..This novel C alpha-s splice variant appeared to have an independent anchor in the human sperm midpiece as it could not be completely solubilized even in the presence of both detergent and cAMP...

Research Grants69

  1. Role of 5-alpha Reductase in Testosterone Actions
    Shalender Bhasin; Fiscal Year: 2003
    ..potent 5-alpha reductase inhibitor (duasteride) that inhibits both type 1 and type 2 steroid 5-alpha -reductase isoenzymes. Healthy young men, 21-40 years of age, will be treated with a long acting GnRH agonist to suppress endogenous ..
  2. Role of 5-alpha Reductase in Testosterone Actions
    Shalendar Bhasin; Fiscal Year: 2007
    ..potent 5-alpha reductase inhibitor (duasteride) that inhibits both type 1 and type 2 steroid 5-alpha -reductase isoenzymes. Healthy young men, 21-40 years of age, will be treated with a long acting GnRH agonist to suppress endogenous ..
  3. Role of 5-alpha Reductase in Testosterone Actions
    Shalender Bhasin; Fiscal Year: 2004
    ..potent 5-alpha reductase inhibitor (duasteride) that inhibits both type 1 and type 2 steroid 5-alpha -reductase isoenzymes. Healthy young men, 21-40 years of age, will be treated with a long acting GnRH agonist to suppress endogenous ..
  4. Role of 5-alpha Reductase in Testosterone Actions
    Shalender Bhasin; Fiscal Year: 2006
    ..potent 5-alpha reductase inhibitor (duasteride) that inhibits both type 1 and type 2 steroid 5-alpha -reductase isoenzymes. Healthy young men, 21-40 years of age, will be treated with a long acting GnRH agonist to suppress endogenous ..
  5. Role of 5-alpha Reductase in Testosterone Actions
    Shalender Bhasin; Fiscal Year: 2005
    ..potent 5-alpha reductase inhibitor (duasteride) that inhibits both type 1 and type 2 steroid 5-alpha -reductase isoenzymes. Healthy young men, 21-40 years of age, will be treated with a long acting GnRH agonist to suppress endogenous ..
  6. Role of 5-alpha Reductase in Testosterone Actions
    Shalender Bhasin; Fiscal Year: 2004
    ..potent 5-alpha reductase inhibitor (duasteride) that inhibits both type 1 and type 2 steroid 5-alpha -reductase isoenzymes. Healthy young men, 21-40 years of age, will be treated with a long acting GnRH agonist to suppress endogenous ..
  7. Effects of age, gender and race on antiepileptic drugs
    James Cloyd; Fiscal Year: 2002
    ..1997-2002), will determine if carbamazepine (CBZ) metabolism, primarily mediated by cytochrome P450 (CYP) isoenzymes 3A4/5 is altered in the elderly, women and African Americans...
  8. ROLE OF S-ADENOSYLMETHIONINE ON UBC9 AND SUMOYLATION IN LIVER CANCER AND ALCOHOLI
    Maria Lauda Tomasi; Fiscal Year: 2010
    ..SAMe biosynthesis due to decreased MAT1A mRNA levels and post-translational inhibition of the MAT1A-encoded isoenzymes. Chronic hepatic SAMe deficiency occurs in MAT1A knockout (KO) mice, which exhibit increased propensity to ..
  9. INTESTINAL AND COLONIC CYTOCHROME P450 GENE EXPRESSION
    Peter Traber; Fiscal Year: 1990
    ..Cytochrome P450 isoenzymes, which are encoded by a large superfamily of related genes play an important role in the metabolism of ..
  10. Effects of age, gender and race on antiepileptic drugs
    James Cloyd; Fiscal Year: 2005
    ..1997-2002), will determine if carbamazepine (CBZ) metabolism, primarily mediated by cytochrome P450 (CYP) isoenzymes 3A4/5 is altered in the elderly, women and African Americans...
  11. ABERRANT SIGNALING IN B-1 LYMPHOCYTES
    THOMAS ROTHSTEIN; Fiscal Year: 1993
    ..First, experiments are proposed to identify differences in the expression and/or activity of PKC isoenzymes between B-1 and B-2 cells in order to determine whether selective expression or activity of a particular ..
  12. REGULATION OF HEME OXYGENASE IN NEONATAL ANIMALS
    David Stevenson; Fiscal Year: 2000
    ..and assess the efficacy of therapeutic agents that inhibit the activity of key enzymes, heme oxygenase isoenzymes 1 and 2 (HO-1,2), in the catalyses of heme to bilirubin...
  13. REGULATION OF HEME OXYGENASE IN NEONATAL ANIMALS
    David Stevenson; Fiscal Year: 1999
    ..and assess the efficacy of therapeutic agents that inhibit the activity of key enzymes, heme oxygenase isoenzymes 1 and 2 (HO-1,2), in the catalyses of heme to bilirubin...
  14. REGULATION OF HEME OXYGENASE IN NEONATAL ANIMALS
    David Stevenson; Fiscal Year: 2001
    ..and assess the efficacy of therapeutic agents that inhibit the activity of key enzymes, heme oxygenase isoenzymes 1 and 2 (HO-1,2), in the catalyses of heme to bilirubin...
  15. REGULATION OF AMINO ACID BIOSYNTHESIS GENES IN PLANTS
    Gloria Coruzzi; Fiscal Year: 1999
    ..The gene families for each enzyme in Arabidopsis contain independently regulated members encoding distinct isoenzymes. Despite decades of in vitro studies conducted in many species, the in vivo roles of GS, GOGAT and GDH ..
  16. REGULATION OF PHOSPHOLIPASE C
    T Harden; Fiscal Year: 1999
    ..The possibility that one or more of the PLC isoenzymes is activated by pertussis toxin- sensitive G-proteins will be examined by reconstitution with recombinant G(..
  17. REGULATION OF PHOSPHOLIPASE C
    T Harden; Fiscal Year: 2001
    ..The possibility that one or more of the PLC isoenzymes is activated by pertussis toxin- sensitive G-proteins will be examined by reconstitution with recombinant G(..
  18. DETOXIFICATION OF XENOBIOTICS IN OCULAR TISSUES
    YOGESH AWASTHI; Fiscal Year: 1990
    ..The multiple isoenzymes of GST present in bovine, human, and rat lens, retina, and cornea will be purified using affinity chromatography,..
  19. REGULATION OF PHOSPHOLIPASE C
    T Harden; Fiscal Year: 2000
    ..The possibility that one or more of the PLC isoenzymes is activated by pertussis toxin- sensitive G-proteins will be examined by reconstitution with recombinant G(..
  20. REGULATION OF PHOSPHOLIPASE C
    T Harden; Fiscal Year: 1993
    ..The possibility that one or more of the PLC isoenzymes is activated by pertussis toxin- sensitive G-proteins will be examined by reconstitution with recombinant G(..
  21. DETOXIFICATION OF XENOBIOTICS IN OCULAR TISSUES
    YOGESH AWASTHI; Fiscal Year: 1993
    ..Abstract): The investigators have demonstrated that a number of glutathione (GSH) S-transferase (GST) isoenzymes are present in lens and other ocular tissues...
  22. DETOXIFICATION OF XENOBIOTICS IN OCULAR TISSUES
    YOGESH AWASTHI; Fiscal Year: 1992
    ..Abstract): The investigators have demonstrated that a number of glutathione (GSH) S-transferase (GST) isoenzymes are present in lens and other ocular tissues...
  23. REGULATION OF PHOSPHOLIPASE C
    T Harden; Fiscal Year: 2002
    ..The possibility that one or more of the PLC isoenzymes is activated by pertussis toxin- sensitive G-proteins will be examined by reconstitution with recombinant G(..
  24. TRANSPORT MECHANISMS IN CORNEAL ENDOTHELIUM
    Jorge Fischbarg; Fiscal Year: 1991
    ..We will attempt to define up to which passage do they maintain that function. Isoenzymes will be assayed for to characterize cells in different passages...
  25. MOLECULAR MECHANISM IN TYPE III GLYCOGEN STORAGE DISEASE
    Yuan Chen; Fiscal Year: 2001
    ..is evaluated by formal muscle testing including Cybex, by EMG and by changes in serum enzymes (CPK, LDH and isoenzymes, SGOT, SGPT)...
  26. MOLECULAR MECHANISM IN TYPE III GLYCOGEN STORAGE DISEASE
    Yuan Chen; Fiscal Year: 2000
    ..is evaluated by formal muscle testing including Cybex, by EMG and by changes in serum enzymes (CPK, LDH and isoenzymes, SGOT, SGPT)...
  27. CHARACTERIZATION AND DEVELOPMENT OF A SEIZURE FOCUS
    Nihal de Lanerolle; Fiscal Year: 1992
    ..The regional distribution of protein kinase C (PKC) and its isoenzymes, and inositol triphosphate (IP3), second messenger systems involved in calcium regulation, will be localized ..
  28. BIOLOGICAL MARKERS IN TREATMENT OF PROSTATE CANCER
    TSANN CHU; Fiscal Year: 1980
    ..Specifically, we propose to measure serum acid phosphatase, alkaline phosphatase and isoenzymes (bone, liver, intestine, "Regan" or placenta) of alkaline phosphatase and polyamines (putrescine, spermidine, ..
  29. CARDIAC MUSCLE CELL DEVELOPMENT AND NUCLEAR PROTEINS
    JASWANT BHORJEE; Fiscal Year: 1990
    ..The neonatal cardiomyocytes in culture exhibit the developmentally regulated profiles of the myosin isoenzymes in the presence of thyroid hormones...
  30. GLUTATHIONE TRANSFERASE: DEFINING AND ALTERING CATALYSIS
    Richard Armstrong; Fiscal Year: 1990
    ..The catalytic versatility of these enzymes results from both the existence of a number of isoenzymes and the fact that each isoenzyme is endowed with a unique yet broad substrate specificity...
  31. TWO TYPES OF MONOAMINE OXIDASE
    Jean Shih; Fiscal Year: 1999
    ..project are to understand the regulation and the in-vivo function of monoamine oxidase (MAO) A and B, which are isoenzymes important for the degradation of monoamine neurotransmitters and biogenic amines...
  32. TWO TYPES OF MONOAMINE OXIDASE
    Jean Shih; Fiscal Year: 2001
    ..project are to understand the regulation and the in-vivo function of monoamine oxidase (MAO) A and B, which are isoenzymes important for the degradation of monoamine neurotransmitters and biogenic amines...
  33. TWO TYPES OF MONOAMINE OXIDASE
    Jean Shih; Fiscal Year: 2002
    ..project are to understand the regulation and the in-vivo function of monoamine oxidase (MAO) A and B, which are isoenzymes important for the degradation of monoamine neurotransmitters and biogenic amines...
  34. Implementing Newborn Screening for Duchenne Muscular Dystrophy in the Community
    Jerry Mendell; Fiscal Year: 2009
    ..the range of creatine kinase (CK) observed on >30,000 anonymous samples, a successful method to measure CK isoenzymes, with particular emphasis on CK-MB, and a reliable method of DNA analysis of the dystrophin gene, with ..
  35. TWO TYPES OF MONOAMINE OXIDASE
    Jean Shih; Fiscal Year: 2007
    ..the[unreadable] regulation and the in-vivo function of monoamine oxidase (MAO) A and B,[unreadable] which are isoenzymes important for the degradation of monoamine[unreadable] neurotransmitters and biogenic amines...
  36. TWO TYPES OF MONOAMINE OXIDASE
    Jean Shih; Fiscal Year: 2000
    ..project are to understand the regulation and the in-vivo function of monoamine oxidase (MAO) A and B, which are isoenzymes important for the degradation of monoamine neurotransmitters and biogenic amines...
  37. STUDIES ON ADENOSINE TRIPHOSPHATE TRANSPHOSPHORYLASES
    THOMAS ALBER; Fiscal Year: 1990
    ..These studies include the following series of enzymes: adenylate kinase isoenzymes from rabbit and calf; nucleoside diphosphokinase from yeast; creatine kinase isoenzymes from rabbit and calf; ..
  38. REGULATION AND FUNCTIONS OF METHIONINE ADENOSYLTRANSFERASE GENES IN LIVER
    SHELLY CHI LOO LU; Fiscal Year: 2011
    ..chronic liver disease have reduced hepatic SAMe level due to decreased expression and activity of MAT1A- encoded isoenzymes. To better understand the in vivo significance, we created the MAT1A knockout (KO) mouse model...
  39. SUBUNIT ASSEMBLY AND FOLDING OF GLUTATHIONE TRANSFERASES
    Richard Armstrong; Fiscal Year: 1999
    ..1) The thermodynamic stabilities and folding pathways of native class mu enzymes (i.e., the rat M1-1 and M2-2 isoenzymes) and a class sigma enzyme (S1-1) from squid will be determined in order to establish the influence of the very ..
  40. CHARACTERIZATION OF RAT KIDNEY GLUTAMINASE ISOENZYMES
    Norman Curthoys; Fiscal Year: 1980
    ..We are now developing procedures to purify the undegraded enzyme. In addition we plan to use immunoprecipitation procedures to study the turnover of the glutaminase in normal and acidotic states...
  41. CONTROL OF METABOLISM IN NORMAL AND DISEASE STATES
    John Williamson; Fiscal Year: 1992
    ..interactions between growth factors and co-mitogenic hormones and 4) receptor coupling to phospholipase C isoenzymes alpha and delta...
  42. CONTROL OF METABOLISM IN NORMAL AND DISEASE STATES
    John Williamson; Fiscal Year: 1991
    ..interactions between growth factors and co-mitogenic hormones and 4) receptor coupling to phospholipase C isoenzymes alpha and delta...
  43. CELLULAR OXIDATIVE TARGETS IN CARDIAC REPERFUSION INJURY
    Anirban Banerjee; Fiscal Year: 1990
    ..Other cytosolic CK isoenzymes (CKcyto) associated with energy utilizing termini use PCr to maintain local ATP levels...
  44. NEUROENDOCRINE BASES OF REPRODUCTIVE BEHAVIOR
    ANNE ETGEN; Fiscal Year: 2000
    ..Specific aim 3 will test the hypothesis that E increases the expression of one or more protein kinase C (PKC) isoenzymes in the HPOA...
  45. MECHANISM OF ANTI-CARCINOGENIC EFFECT OF ANTIOXIDANTS
    YOGESH AWASTHI; Fiscal Year: 1990
    ..We have demonstrated that these antioxidants induce specific glutathione S-transferase (GST) isoenzymes of rodent lung...
  46. STRUCTURAL STUDIES ON DIHYDROFOLATE REDUCTASE
    RAYMOND BLAKLEY; Fiscal Year: 1990
    ..measurements of rate constants for inhibitor binding to and release from DHFR from Streptococcus faecium (two isoenzymes, SFDHFR 1 and 2), Lactobacillus casei, Escherichia coli, bovine liver, chicken liver and WIL2 human lymphoblasts...
  47. Flavocoxid: A Medical Food Therapy for Osteoarthritis
    Sarah Morgan; Fiscal Year: 2004
    ..Conventional non-steroidal anti-inflammatory drugs (NSAIDs) inhibit COX-1 and COX-2 isoenzymes, but have a marginal or no effect on 5-LO...
  48. REGULATION OF AMINO ACID BIOSYNTHESIS GENES IN PLANTS
    Gloria Coruzzi; Fiscal Year: 2003
    ..Using molecular-genetic approaches in Arabidopsis, we identified isoenzymes of GS, GOGAT and GDH that control the assimilation of inorganic-N into Glu/Gln, key amino acids used to ..
  49. REGULATION OF AMINO ACID BIOSYNTHESIS GENES IN PLANTS
    Gloria Coruzzi; Fiscal Year: 2002
    ..Using molecular-genetic approaches in Arabidopsis, we identified isoenzymes of GS, GOGAT and GDH that control the assimilation of inorganic-N into Glu/Gln, key amino acids used to ..
  50. REGULATION OF AMINO ACID BIOSYNTHESIS GENES IN PLANTS
    Gloria Coruzzi; Fiscal Year: 2003
    ..Using molecular-genetic approaches in Arabidopsis, we identified isoenzymes of GS, GOGAT and GDH that control the assimilation of inorganic-N into Glu/Gln, key amino acids used to ..
  51. REGULATION OF AMINO ACID BIOSYNTHESIS GENES IN PLANTS
    Gloria Coruzzi; Fiscal Year: 2002
    ..Using molecular-genetic approaches in Arabidopsis, we identified isoenzymes of GS, GOGAT and GDH that control the assimilation of inorganic-N into Glu/Gln, key amino acids used to ..
  52. REGULATION OF AMINO ACID BIOSYNTHESIS GENES IN PLANTS
    Gloria Coruzzi; Fiscal Year: 2000
    ..Using molecular-genetic approaches in Arabidopsis, we identified isoenzymes of GS, GOGAT and GDH that control the assimilation of inorganic-N into Glu/Gln, key amino acids used to ..
  53. REGULATION OF AMINO ACID BIOSYNTHESIS GENES IN PLANTS
    Gloria Coruzzi; Fiscal Year: 2002
    ..Using molecular-genetic approaches in Arabidopsis, we identified isoenzymes of GS, GOGAT and GDH that control the assimilation of inorganic-N into Glu/Gln, key amino acids used to ..
  54. REGULATION OF AMINO ACID BIOSYNTHESIS GENES IN PLANTS
    Gloria Coruzzi; Fiscal Year: 2001
    ..Using molecular-genetic approaches in Arabidopsis, we identified isoenzymes of GS, GOGAT and GDH that control the assimilation of inorganic-N into Glu/Gln, key amino acids used to ..
  55. Prostate cancer prevention by selenium supplementation
    Rayudu Gopalakrishna; Fiscal Year: 2010
    ..Advanced tumor cells escape the cancer-preventive actions of selenium via the increased expression of PKC isoenzymes (Se- sensitive 1or Se-resistant 5) and selenoprotein (TR) which naturally occurs in tumor progression...