metalloproteases

Summary

Summary: Proteases which use a metal, normally ZINC, in the catalytic mechanism. This group of enzymes is inactivated by metal CHELATORS.

Top Publications

  1. pmc Regulation of OPA1 processing and mitochondrial fusion by m-AAA protease isoenzymes and OMA1
    Sarah Ehses
    Institute for Genetics, University of Cologne, Germany
    J Cell Biol 187:1023-36. 2009
  2. ncbi Domain loss facilitates accelerated evolution and neofunctionalization of duplicate snake venom metalloproteinase toxin genes
    Nicholas R Casewell
    Alistair Reid Venom Research Unit, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
    Mol Biol Evol 28:2637-49. 2011
  3. pmc ORMDL3 is an inducible lung epithelial gene regulating metalloproteases, chemokines, OAS, and ATF6
    Marina Miller
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 109:16648-53. 2012
  4. pmc Synaptic autoregulation by metalloproteases and γ-secretase
    Sophie Restituito
    Department of Biochemistry, New York University Langone Medical Center, New York, New York 10016, USA
    J Neurosci 31:12083-93. 2011
  5. ncbi Scyphozoan jellyfish venom metalloproteinases and their role in the cytotoxicity
    Hyunkyoung Lee
    Department of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju 660 701, South Korea
    Toxicon 58:277-84. 2011
  6. pmc PINK1 is selectively stabilized on impaired mitochondria to activate Parkin
    Derek P Narendra
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA
    PLoS Biol 8:e1000298. 2010
  7. ncbi Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis
    Tse Siang Kang
    Department of Pharmacy, National University of Singapore, Singapore
    FEBS J 278:4544-76. 2011
  8. pmc Androgen receptor expresion in breast cancer: relationship with clinicopathological characteristics of the tumors, prognosis, and expression of metalloproteases and their inhibitors
    Luis O Gonzalez
    Unidad de Investigacion, Hospital de Jove, Gijon, Spain
    BMC Cancer 8:149. 2008
  9. pmc The InhA metalloproteases of Bacillus cereus contribute concomitantly to virulence
    Elisabeth Guillemet
    Unité Génétique Microbienne et Environnement, INRA, La Minière, 78285 Guyancourt Cedex, France
    J Bacteriol 192:286-94. 2010
  10. ncbi Structural considerations of the snake venom metalloproteinases, key members of the M12 reprolysin family of metalloproteinases
    Jay W Fox
    Department of Microbiology, Health Sciences Center, University of Virginia, Charlottesville, VA 22908 0734, USA
    Toxicon 45:969-85. 2005

Detail Information

Publications278 found, 100 shown here

  1. pmc Regulation of OPA1 processing and mitochondrial fusion by m-AAA protease isoenzymes and OMA1
    Sarah Ehses
    Institute for Genetics, University of Cologne, Germany
    J Cell Biol 187:1023-36. 2009
    ..Our findings link distinct peptidases to constitutive and induced OPA1 processing and shed new light on the pathogenesis of neurodegenerative disorders associated with mutations in m-AAA protease subunits...
  2. ncbi Domain loss facilitates accelerated evolution and neofunctionalization of duplicate snake venom metalloproteinase toxin genes
    Nicholas R Casewell
    Alistair Reid Venom Research Unit, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
    Mol Biol Evol 28:2637-49. 2011
    ....
  3. pmc ORMDL3 is an inducible lung epithelial gene regulating metalloproteases, chemokines, OAS, and ATF6
    Marina Miller
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 109:16648-53. 2012
    ..Transfection of ORMDL3 in human bronchial epithelial cells in vitro induced expression of metalloproteases (MMP-9, ADAM-8), CC chemokines (CCL-20), CXC chemokines (IL-8, CXCL-10, CXCL-11), oligoadenylate synthetases (..
  4. pmc Synaptic autoregulation by metalloproteases and γ-secretase
    Sophie Restituito
    Department of Biochemistry, New York University Langone Medical Center, New York, New York 10016, USA
    J Neurosci 31:12083-93. 2011
    The proteolytic machinery comprising metalloproteases and γ-secretase, an intramembrane aspartyl protease involved in Alzheimer's disease, cleaves several substrates in addition to the extensively studied amyloid precursor protein...
  5. ncbi Scyphozoan jellyfish venom metalloproteinases and their role in the cytotoxicity
    Hyunkyoung Lee
    Department of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju 660 701, South Korea
    Toxicon 58:277-84. 2011
    ..In conclusion, the present report proposes a novel finding of Scyphozoan jellyfish venom metalloproteinases and their potential role in the cytotoxicity...
  6. pmc PINK1 is selectively stabilized on impaired mitochondria to activate Parkin
    Derek P Narendra
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA
    PLoS Biol 8:e1000298. 2010
    ..In addition, they support a novel model for the negative selection of damaged mitochondria, in which PINK1 signals mitochondrial dysfunction to Parkin, and Parkin promotes their elimination...
  7. ncbi Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis
    Tse Siang Kang
    Department of Pharmacy, National University of Singapore, Singapore
    FEBS J 278:4544-76. 2011
    ..The structures of inhibitor-enzyme complexes provide ideal platforms for the design of potent inhibitors which are useful in the development of prototypes and lead compounds with potential therapeutic applications...
  8. pmc Androgen receptor expresion in breast cancer: relationship with clinicopathological characteristics of the tumors, prognosis, and expression of metalloproteases and their inhibitors
    Luis O Gonzalez
    Unidad de Investigacion, Hospital de Jove, Gijon, Spain
    BMC Cancer 8:149. 2008
    ..cancer, the tumor expression of androgen receptors (AR), its relationship with clinicopathological characteristics and with the expression of several matrix metalloproteases (MMPs) and their inhibitors (TIMPs), as well as with prognosis.
  9. pmc The InhA metalloproteases of Bacillus cereus contribute concomitantly to virulence
    Elisabeth Guillemet
    Unité Génétique Microbienne et Environnement, INRA, La Minière, 78285 Guyancourt Cedex, France
    J Bacteriol 192:286-94. 2010
    ..To study this aspect of pathogenesis, we focused on three metalloproteases, InhA1, InhA2, and InhA3, which share more than 66% identity...
  10. ncbi Structural considerations of the snake venom metalloproteinases, key members of the M12 reprolysin family of metalloproteinases
    Jay W Fox
    Department of Microbiology, Health Sciences Center, University of Virginia, Charlottesville, VA 22908 0734, USA
    Toxicon 45:969-85. 2005
    ..What the reader will observe is that there are very interesting structural features displayed by the various SVMP classes and subclasses that provide insight into their functional characteristics...
  11. pmc CLCAs - a family of metalloproteases of intriguing phylogenetic distribution and with cases of substituted catalytic sites
    Anna Lenart
    Department of Cellular and Molecular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland
    PLoS ONE 8:e62272. 2013
    The zinc-dependent metalloproteases with His-Glu-x-x-His (HExxH) active site motif, zincins, are a broad group of proteins involved in many metabolic and regulatory functions, and found in all forms of life...
  12. ncbi Human complement activation and anaphylatoxins generation induced by snake venom toxins from Bothrops genus
    Giselle Pidde-Queiroz
    Immunochemistry Laboratory, Butantan Institute, Av Prof Vital Brazil, 1500, CEP 05503 900, Sao Paulo, Brazil
    Mol Immunol 47:2537-44. 2010
    ....
  13. ncbi The expression and regulation of ADAMTS-1, -4, -5, -9, and -15, and TIMP-3 by TGFbeta1 in prostate cells: relevance to the accumulation of versican
    N A Cross
    Academic Urology Unit, Division of Clinical Sciences South, University of Sheffield, Royal Hallamshire Hospital, Sheffield, United Kingdom
    Prostate 63:269-75. 2005
    ..A sub-group of the ADAMTS lineage of metalloproteases possess versican-degrading properties and are potential regulators of proteoglycan accumulation associated ..
  14. ncbi Expression profiling, gene silencing and transcriptional networking of metzincin metalloproteases in the cattle tick, Rhipicephalus (Boophilus) microplus
    Annette Christi Barnard
    Department of Biochemistry, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, 0002, South Africa
    Vet Parasitol 186:403-14. 2012
    ..In this study we identified metzincin metalloproteases from Rhipicephalus microplus as potential vaccine candidates since they are implicated as essential to blood-..
  15. ncbi Isolation and structural characterization of a new fibrin(ogen)olytic metalloproteinase from Bothrops moojeni snake venom
    Carolina P Bernardes
    Instituto de Ciencias Biomedicas, Universidade Federal de Uberlandia, 38400 902 Uberlândia MG, Brazil
    Toxicon 51:574-84. 2008
    ..Since the BmooMPalpha-I enzyme was found to cause defibrinogenation when administered i.p. on mice, it is expected that it may be of medical interest as a therapeutic agent in the treatment and prevention of arterial thrombosis...
  16. pmc Similar active sites in lysostaphins and D-Ala-D-Ala metallopeptidases
    Matthias Bochtler
    IIMCB, Trojdena 4, 02 109 Warsaw, Poland
    Protein Sci 13:854-61. 2004
    ....
  17. ncbi Bothrops jararaca venom gland transcriptome: analysis of the gene expression pattern
    Daniela A P Cidade
    Departamento de Bioquimica, Universidade do Estado do Rio de Janeiro, CEP 20551 013 Rio de Janeiro, RJ, Brasil
    Toxicon 48:437-61. 2006
    ....
  18. ncbi Evolutionary trends in venom composition in the western rattlesnakes (Crotalus viridis sensu lato): toxicity vs. tenderizers
    Stephen P Mackessy
    School of Biological Sciences, University of Northern Colorado, 501 20th St, CB 92, Greeley, CO 80639 0017, USA
    Toxicon 55:1463-74. 2010
    ..The functional significance of these biochemical characteristics likely relates to characteristics of prey consumed, and venoms with low metalloproteinase activity may constrain snake prey selection or foraging activity patterns...
  19. pmc Human meprin alpha and beta homo-oligomers: cleavage of basement membrane proteins and sensitivity to metalloprotease inhibitors
    Markus N Kruse
    Institute of Zoophysiology, University of Munster, Hindenburgplatz 55, D 48143 Munster, Germany
    Biochem J 378:383-9. 2004
    ..Therapeutic targets for these inhibitors are MMPs (matrix metalloproteases), TACE (tumour necrosis factor alpha-converting enzyme) and angiotensin-converting enzyme respectively...
  20. ncbi Degradation of circulating von Willebrand factor and its regulator ADAMTS13 implicates secreted Bacillus anthracis metalloproteases in anthrax consumptive coagulopathy
    Myung Chul Chung
    National Center for Biodefense and Infectious Diseases, College of Sciences, George Mason University, 10900 University Boulevard, Manassas, VA 20110, USA
    J Biol Chem 283:9531-42. 2008
    ..We previously demonstrated that purified anthrax neutral metalloproteases Npr599 and InhA are capable of cleaving a variety of host structural and regulatory proteins...
  21. ncbi The Arabidopsis FtsH metalloprotease gene family: interchangeability of subunits in chloroplast oligomeric complexes
    Fei Yu
    Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA
    Plant J 37:864-76. 2004
    ..A threshold model is presented to explain the pattern of variegation in var2 in which AtFtsH8 provides a compensating activity in the green sectors of the mutant...
  22. ncbi The novel metalloproteinase atroxlysin-I from Peruvian Bothrops atrox (Jergón) snake venom acts both on blood vessel ECM and platelets
    Eladio F Sanchez
    Research and Development Center, Ezequiel Dias Foundation, 30510 010 Belo Horizonte, Brazil
    Arch Biochem Biophys 496:9-20. 2010
    ..Complementarily, the laminin and collagen binding integrins alpha7beta1 and alpha1beta1 were cleaved by atroxlysin. Even without catalytic activity atroxlysin-I inhibited collagen- and ADP-triggered platelet aggregation...
  23. ncbi Expression profiling of metalloproteinases and tissue inhibitors of metalloproteinases in normal and degenerate human achilles tendon
    Gavin C Jones
    Rheumatology Research Unit, Addenbrooke s Hospital, Cambridge, UK
    Arthritis Rheum 54:832-42. 2006
    ....
  24. ncbi Effects of glucosamine hydrochloride on the production of prostaglandin E2, nitric oxide and metalloproteases by chondrocytes and synoviocytes in osteoarthritis
    H Nakamura
    Department of Bioregulation, St Marianna University School of Medicine, Kawasaki, Kanagawa, Japan
    Clin Exp Rheumatol 22:293-9. 2004
    To determine the response of glucosamine hydrochloride on chondrocytes and synoviocytes in terms of prostaglandin E2 (PGE2), nitric oxide (NO) and matrix metalloproteases (MMPs).
  25. ncbi Degradomics: systems biology of the protease web. Pleiotropic roles of MMPs in cancer
    Christopher M Overall
    The UBC Centre for Blood Research, Life Sciences Institute, CBCRA Program in Breast Cancer Metastasis, Department of Oral Biological, University of British Columbia, Vancouver, B C, Canada, V6T 1Z3
    Cancer Metastasis Rev 25:69-75. 2006
    ..These sophisticated cellular control functions highlight new intervention points in multiple pathways to treat early stage cancer...
  26. ncbi Neutralization of the haemorrhagic activities of viperine snake venoms and venom metalloproteinases using synthetic peptide inhibitors and chelators
    J M Howes
    Alistair Reid Venom Research Unit, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
    Toxicon 49:734-9. 2007
    ....
  27. ncbi Snake venom fibrin(ogen)olytic enzymes
    S Swenson
    Department of Biochemistry and Molecular Biology, Norris Comprehensive Cancer Centre, University of Southern California, Keck School of Medicine, Los Angeles, CA 90033, USA
    Toxicon 45:1021-39. 2005
    ....
  28. pmc Changes in matrix protein biochemistry and the expression of mRNA encoding matrix proteins and metalloproteinases in posterior tibialis tendinopathy
    Anthony N Corps
    Rheumatology Research Unit, Addenbrooke s Hospital, Cambridge, UK
    Ann Rheum Dis 71:746-52. 2012
    ..In this study, the authors compared normal PTT, stage II dysfunctional PTT and replacement FDLT, aiming to define changes in collagen modification, glycosaminoglycan (GAG) and the expression of matrix and metalloproteinase mRNA...
  29. pmc Role of collagens and perlecan in microvascular stability: exploring the mechanism of capillary vessel damage by snake venom metalloproteinases
    Teresa Escalante
    Instituto Clodomiro Picado, Facultad de Microbiologia, Universidad de Costa Rica, San Jose, Costa Rica
    PLoS ONE 6:e28017. 2011
    ..These results underscore the key role played by these ECM components in the mechanical stability of microvessels...
  30. pmc The structure of Mlc titration factor A (MtfA/YeeI) reveals a prototypical zinc metallopeptidase related to anthrax lethal factor
    Qingping Xu
    Joint Center for Structural Genomics, SLAC National Accelerator Laboratory, Menlo Park, CA, USA
    J Bacteriol 194:2987-99. 2012
    ..These results clearly provide support for MtfA as a prototypical zinc metallopeptidase (gluzincin clan)...
  31. ncbi Snake venom metalloproteinases
    Francis S Markland
    University of Southern California, Keck School of Medicine, Cancer Research Laboratory 106, 1303 N Mission Rd, Los Angeles, CA 90033, USA
    Toxicon 62:3-18. 2013
    ..The "Met-turn" structure contains a conserved Met residue that forms a hydrophobic basement for the three zinc-binding histidines in the consensus sequence...
  32. pmc The murine goblet cell protein mCLCA3 is a zinc-dependent metalloprotease with autoproteolytic activity
    Melanie K Bothe
    Department of Veterinary Pathology, Freie Universitaet Berlin, Berlin, Germany
    Mol Cells 32:535-41. 2011
    ..a conserved HEXXH zinc-binding amino acid motif has been identified, suggesting a role for CLCA proteins as metalloproteases. Here, we have characterized the cleavage and autoproteolytic activity of the murine model protein mCLCA3, ..
  33. pmc The potential role of metalloproteinases in neurogenesis in the gerbil hippocampus following global forebrain ischemia
    Luiza Wojcik-Stanaszek
    NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland
    PLoS ONE 6:e22465. 2011
    ....
  34. ncbi Activation of the epidermal growth factor receptor (EGFR) by a novel metalloprotease pathway
    David A Bergin
    Respiratory Research Division, Department of Medicine, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin 9, Ireland
    J Biol Chem 283:31736-44. 2008
    ..The data describe a previously unidentified lung metalloprotease meprin alpha, and its role in NE-induced EGFR and TLR4 activation and IL-8 production...
  35. ncbi Characterization of secreted proteases of Paenibacillus larvae, potential virulence factors involved in honeybee larval infection
    Karina Antúnez
    Departamento de Microbiologia, Instituto de Investigaciones Biologicas Clemente Estable, Avda Italia 3318, CP 11600 Montevideo, Uruguay
    J Invertebr Pathol 102:129-32. 2009
    ..larvae. Inhibition assays confirmed the presence of metalloproteases. Two different proteases patterns (PP1 and PP2) were identified in a collection of P...
  36. pmc The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus)
    Darin R Rokyta
    Department of Biological Science, Florida State University, Tallahassee, FL 32306 4295, USA
    BMC Genomics 13:312. 2012
    ....
  37. pmc Trypanosoma cruzi GP63 proteins undergo stage-specific differential posttranslational modification and are important for host cell infection
    Manjusha M Kulkarni
    Division of Infectious Diseases and Center for Microbial Interface Biology, The Ohio State University, Biomedical Research Tower, Rm 1012, 460 W 12th Ave, Columbus, OH 43210 1240, USA
    Infect Immun 77:2193-200. 2009
    The protozoan Trypanosoma cruzi expresses multiple isoforms of the GP63 family of metalloproteases. Polyclonal antiserum against recombinant GP63 of T. cruzi (TcGP63) was used to study TcGP63 expression and localization in this organism...
  38. pmc Self-cleavage of human CLCA1 protein by a novel internal metalloprotease domain controls calcium-activated chloride channel activation
    Zeynep Yurtsever
    Biochemistry Program, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 287:42138-49. 2012
    ..These data provide both a mechanistic basis for CLCA1 self-cleavage and a novel mechanism for regulation of chloride channel activity specific to the mucosal interface...
  39. ncbi Effect of retinal laser photocoagulation on the activity of metalloproteinases and the alpha(2)-macroglobulin proteolytic state in the vitreous of eyes with proliferative diabetic retinopathy
    Maria C Sanchez
    Centro de Investigaciones en Bioquímica Clínica e Inmunología CIBICI CONICET Departamento de Bioquímica Clínica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Ciudad Universitaria, Cordoba, Argentina
    Exp Eye Res 85:644-50. 2007
    ..In addition, we suggest that alpha(2)M plays a key role in the control and regulation of the ocular neovascularization involved in the pathogenesis of ischemic retinal diseases such as PDR...
  40. ncbi Metalloproteases and rotator cuff disease
    Angelo Del Buono
    Department of Orthopaedic and Trauma Surgery, Campus Biomedico, University of Rome, Rome, Italy
    J Shoulder Elbow Surg 21:200-8. 2012
    ..Further studies are needed to better define the mechanism of action, and whether these new strategies are safe and effective in larger models...
  41. ncbi Proteolysis of Xenopus laevis egg envelope ZPA triggers envelope hardening
    Leann L Lindsay
    Department of Animal Science, University of California, Davis, CA 95616, United States
    Biochem Biophys Res Commun 324:648-54. 2004
    ..We propose a mechanism for egg envelope hardening involving ZPA proteolysis by an egg metalloprotease as a triggering event followed by induction of global conformational changes in egg envelope glycoproteins...
  42. pmc Metalloproteinases in Drosophila to humans that are central players in developmental processes
    Alison Muir
    Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53792, USA
    J Biol Chem 286:41905-11. 2011
    ....
  43. pmc Novel venom gene discovery in the platypus
    Camilla M Whittington
    Faculty of Veterinary Science, The University of Sydney, Regimental Crescent, Camperdown, NSW 2006, Australia
    Genome Biol 11:R95. 2010
    ..We have constructed and sequenced a cDNA library from an active platypus venom gland to identify the remaining components...
  44. pmc JAMM: a metalloprotease-like zinc site in the proteasome and signalosome
    Xavier I Ambroggio
    Division of Biology, California Institute of Technology, Pasadena, California, USA
    PLoS Biol 2:E2. 2004
    ..The active site-like architecture specified by the JAMM motif motivates structure-based approaches to the study of JAMM domain proteins and the development of therapeutic proteasome and signalosome inhibitors...
  45. ncbi UV-induced EGFR signal transactivation is dependent on proligand shedding by activated metalloproteases in skin cancer cell lines
    Bhuminder Singh
    Department of Molecular Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Int J Cancer 124:531-9. 2009
    ..Using RNAi this EGFR activation was further shown to depend on the metalloproteases ADAM9 and ADAM17 in SCC-9 cells...
  46. ncbi A membrane metalloprotease participates in the sequential degradation of a Caulobacter polarity determinant
    Joseph C Chen
    Department of Developmental Biology, Stanford University School of Medicine, 300 Beckman Center, 279 Campus Drive, Stanford, CA 94305 5329, USA
    Mol Microbiol 55:1085-103. 2005
    ..MmpA belongs to the site-2 protease (S2P) family of membrane-embedded zinc metalloproteases, which includes SpoIVFB and YluC of Bacillus subtilis and YaeL of Escherichia coli...
  47. ncbi ADAMs, cell migration and cancer
    Joaquin Arribas
    Medical Oncology Research Program, Vall d Hebron University Hospital Research Institute, 08035, Barcelona, Spain
    Cancer Metastasis Rev 25:57-68. 2006
  48. pmc Metalloproteases regulate T-cell proliferation and effector function via LAG-3
    Nianyu Li
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    EMBO J 26:494-504. 2007
    ..We show that LAG-3 cleavage is mediated by two transmembrane metalloproteases, ADAM10 and ADAM17, with the activity of both modulated by two distinct T-cell receptor (TCR) signaling-..
  49. ncbi A family of putative metalloproteases in the salivary glands of the tick Ixodes ricinus
    Yves Decrem
    IBMM, Institut de Biologie et Médecine Moléculaires, Universite Libre de Bruxelles, Gosselies, Belgium
    FEBS J 275:1485-99. 2008
    ..Here, we describe a multigene family coding for five putative salivary metalloproteases induced during the blood meal of Ixodes ricinus...
  50. ncbi Activation of plasminogen activator inhibitor implicates protease InhA in the acute-phase response to Bacillus anthracis infection
    Myung Chul Chung
    National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, VA 20110, USA
    J Med Microbiol 58:737-44. 2009
    ....
  51. pmc Isolation and characterization of metalloproteases with a novel domain structure by construction and screening of metagenomic libraries
    Tanja Waschkowitz
    Abteilung Genomische und Angewandte Mikrobiologie, Institut für Mikrobiologie und Genetik der Georg August Universität, Grisebachstr 8, 37077 Gottingen, Germany
    Appl Environ Microbiol 75:2506-16. 2009
    ..of two prepeptidase C-terminal domains and one proprotein convertase P domain, is novel for bacterial metalloproteases. Studies with derivatives of MprA and MprB revealed that the C-terminal extension is not essential for ..
  52. ncbi An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensin-converting enzyme family that lacks a metallopeptidase active site
    Darren R Brooks
    Molecular and Cellular Biosciences Research, Faculty of Biological Sciences, Miall Building, University of Leeds, Leeds LS2 9JT, United Kingdom
    J Biol Chem 278:52340-6. 2003
    ....
  53. ncbi A new protein structure of P-II class snake venom metalloproteinases: it comprises metalloproteinase and disintegrin domains
    Run Qiang Chen
    Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223, PR China
    Biochem Biophys Res Commun 310:182-7. 2003
    ....
  54. pmc Regulation of skeletal muscle oxidative capacity and insulin signaling by the mitochondrial rhomboid protease PARL
    Anthony E Civitarese
    Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA
    Cell Metab 11:412-26. 2010
    ..We propose that lower PARL expression may contribute to the mitochondrial abnormalities seen in aging and T2DM...
  55. ncbi Synergistic inhibition with a dual epidermal growth factor receptor/HER-2/neu tyrosine kinase inhibitor and a disintegrin and metalloprotease inhibitor
    Lois Witters
    Department of Medicine, The Milton S Hershey Medical Center Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Cancer Res 68:7083-9. 2008
    ..These results suggest that there may be an additional clinical benefit of combining agents that target the ErbB pathways at multiple points...
  56. ncbi Identification and characterization of human archaemetzincin-1 and -2, two novel members of a family of metalloproteases widely distributed in Archaea
    Araceli Diaz-Perales
    Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina, Instituto Universitario de Oncologia, Universidad de Oviedo, 33006 Oviedo, Spain
    J Biol Chem 280:30367-75. 2005
    ..We report here the identification of two new human metalloproteases that have been called archaemetzincin-1 (AMZ1) and archaemetzincin-2 (AMZ2) to emphasize their close ..
  57. ncbi Hemorrhage induced by snake venom metalloproteinases: biochemical and biophysical mechanisms involved in microvessel damage
    Jose Maria Gutierrez
    Instituto Clodomiro Picado, Facultad de Microbiologia, Universidad de Costa Rica, San Jose, Costa Rica
    Toxicon 45:997-1011. 2005
    ..In addition, endothelial cells become more susceptible to blood flow-dependent shear stress, which further contributes to capillary wall disruption...
  58. pmc Regulation of the mitochondrial dynamin-like protein Opa1 by proteolytic cleavage
    Lorena Griparic
    Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
    J Cell Biol 178:757-64. 2007
    ..We conclude that Yme1 constitutively regulates a subset of Opa1 isoforms and an unknown mitochondrial morphology protein, whereas the loss of membrane potential induces the further proteolysis of Opa1...
  59. ncbi Backbone flexibility controls the activity and specificity of a protein-protein interface: specificity in snake venom metalloproteases
    Hannes G Wallnoefer
    Institute of General, Inorganic and Theoretical Chemistry, Faculty of Chemistry and Pharmacy, University of Innsbruck, Innrain 52a, A 6020 Innsbruck, Austria
    J Am Chem Soc 132:10330-7. 2010
    ..A perfect paradigm to study such multispecific protein-protein interfaces are snake venom metalloproteases (SVMPs)...
  60. ncbi Analysis of Bothrops jararacussu venomous gland transcriptome focusing on structural and functional aspects: I--gene expression profile of highly expressed phospholipases A2
    Simone Kashima
    Unidade de Biotecnologia, Universidade de Ribeirao Preto, UNAERP, 14096 380 Ribeirão Preto, SP, Brazil
    Biochimie 86:211-9. 2004
    ..Adjoining this very abundant class of proteins we found 88 transcripts codifying for putative sequences of metalloproteases, which after clustering and assembling resulted in three full-length sequences: BOJUMET-I, BOJUMET-II and ..
  61. pmc Collagens, proteoglycans, MMP-2, MMP-9 and TIMPs in human achilles tendon rupture
    Evgenia Karousou
    Department of Experimental Biomedical and Clinical Sciences DSBSC, University of Insubria, Varese, Italy
    Clin Orthop Relat Res 466:1577-82. 2008
    ..expression of collagen Type I, decorin, and versican including enzymes involved in their metabolism as matrix metalloproteases (MMP-2 and -9) and tissue inhibitory of metalloproteinase (TIMP-1 and -2) using real-time PCR, zymography and ..
  62. ncbi Expression of metalloproteases and their inhibitors in different histological types of breast cancer
    J M del Casar
    Unidad de Investigacion, Fundación Hospital de Jove, 33290 Gijón, Asturias, Spain
    J Cancer Res Clin Oncol 136:811-9. 2010
    b>Metalloproteases (MMPs) and their tissue inhibitors of metalloproteases (TIMPs) are involved in several key aspects of tumoral growth, invasion and metastasis...
  63. ncbi Venomic and antivenomic analyses of the Central American coral snake, Micrurus nigrocinctus (Elapidae)
    JULIAN FERNANDEZ
    Instituto Clodomiro Picado, Facultad de Microbiología, Escuela de Medicina, Universidad de Costa Rica, San José, Costa Rica
    J Proteome Res 10:1816-27. 2011
    ..nigrocinctus equine antivenom revealed differences in immunorecognition of venom proteins that correlate with their molecular mass, with the weakest recognition observed toward 3FTxs...
  64. pmc On the relevance of the Met-turn methionine in metzincins
    Cynthia Tallant
    Proteolysis Laboratory, Department of Structural Biology, Molecular Biology Institute of Barcelona, Consejo Superior de Investigaciones Cientificas, Barcelona Science Park, Helix Building, c Baldiri Reixac, 15 21, E 08028 Barcelona, Spain
    J Biol Chem 285:13951-7. 2010
    ..Replacement of the methionine with residues that deviate in size, side-chain conformation, and chemical properties impairs the plug-core interaction and prejudices molecular stability and activity...
  65. ncbi Extracellular proteases of Acanthamoeba castellanii (encephalitis isolate belonging to T1 genotype) contribute to increased permeability in an in vitro model of the human blood-brain barrier
    Selwa Alsam
    School of Biological and Chemical Sciences, Birkbeck College, University of London, London WC1E 7HX, UK
    J Infect 51:150-6. 2005
    ..Objectives of this study were to determine the effects of Acanthamoeba on the permeability of an in vitro blood-brain barrier model and factors contributing to these changes...
  66. pmc Role of metalloproteases in vaccinia virus epitope processing for transporter associated with antigen processing (TAP)-independent human leukocyte antigen (HLA)-B7 class I antigen presentation
    Elena Lorente
    Instituto de Salud Carlos III, Centro Nacional de Microbiologia, 28220 Majadahonda Madrid, Spain
    J Biol Chem 287:9990-10000. 2012
    ..These data may explain why TAP-deficient individuals live normal life spans without any increased susceptibility to viral infections...
  67. ncbi Substrate specificity of a metalloprotease of the pappalysin family revealed by an inhibitor and a product complex
    Raquel García-Castellanos
    Departament de Biologia Estructural, Institut de Biologia Molecular de Barcelona, CSIC, c Jordi Girona, 18 26, E 08034 Barcelona, Spain
    Arch Biochem Biophys 457:57-72. 2007
    ..of insulin-like growth factors and the founding member of the pappalysin family within the metzincin clan of metalloproteases. We have recently identified an archaeal relative, ulilysin, encompassing only the protease domain...
  68. pmc Endothelin-converting enzymes and related metalloproteases in Alzheimer's disease
    Javier Pacheco-Quinto
    Biomedical Research Institute of New Jersey, MidAtlantic Neonatology Associates, and Atlantic Health System, Morristown, NJ, USA
    J Alzheimers Dis 33:S101-10. 2013
    ....
  69. ncbi Expression of mRNAs coding for VAP1/crotastatin-like metalloproteases in the venom glands of three South American pit vipers assessed by quantitative real-time PCR
    N A C Tavares
    Department of Biochemistry and Laboratory of Immunopathology Keizo Asami LIKA, Federal University of Pernambuco, Av Professor Moraes Rego 1235, Cidade Universitaria, Recife, PE 50670 901, Brazil
    Toxicon 52:897-907. 2008
    Snake venom metalloproteases encompass a large family of toxins, with approximately 200 members already catalogued, which exhibit a diversity of structures and biological functions...
  70. ncbi Molecular characterization and comparative study of 6 salivary gland metalloproteases from the hard tick, Haemaphysalis longicornis
    Thasaneeya Harnnoi
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    Comp Biochem Physiol B Biochem Mol Biol 147:93-101. 2007
    Six genes encoding metalloproteases were identified from the salivary gland of the hard tick, Haemaphysalis longicornis...
  71. ncbi [Metalloproteases, vascular remodeling and atherothrombotic syndromes]
    Jose A Rodriguez
    Laboratorio de Aterosclerosis, Area de Ciencias Cardiovasculares, CIMA Universidad de Navarra, Pamplona, Espana
    Rev Esp Cardiol 60:959-67. 2007
    ....
  72. pmc Mitochondrial respiratory chain dysfunction modulates metalloproteases -1, -3 and -13 in human normal chondrocytes in culture
    Berta Cillero-Pastor
    Rheumatology Division, INIBIC Hospital Universitario A Coruña, A Coruna, Spain
    BMC Musculoskelet Disord 14:235. 2013
    ..In this study we have investigated the relationship between the MRC dysfunction and the regulation of metalloproteases (MMPs) in human normal chondrocytes in culture.
  73. pmc The substrate degradome of meprin metalloproteases reveals an unexpected proteolytic link between meprin β and ADAM10
    Tamara Jefferson
    Institute of Biochemistry, Christian Albrechts University, 24118, Kiel, Germany
    Cell Mol Life Sci 70:309-33. 2013
    The in vivo roles of meprin metalloproteases in pathophysiological conditions remain elusive. Substrates define protease roles...
  74. ncbi The role of serine- and metalloproteases in Nasonia vitripennis venom in cell death related processes towards a Spodoptera frugiperda Sf21 cell line
    Ellen M Formesyn
    Laboratory of Zoophysiology, Ghent University, B 9000 Ghent, Belgium
    J Insect Physiol 59:795-803. 2013
    ..Two protease families, serine proteases and metalloproteases were examined for their possible cytotoxic functions in the Spodoptera frugiperda (Sf21) cell line using ..
  75. ncbi Secreted proteases from dermatophytes
    Michel Monod
    Service de Dermatologie et Venereologie, Laboratoire de Mycologie, BT422, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland
    Mycopathologia 166:285-94. 2008
    ..and are members of two large protein families, the subtilisins (serine proteases) and the fungalysins (metalloproteases). In addition, dermatophytes excrete sulphite as a reducing agent...
  76. ncbi Extracellular metalloproteases from bacteria
    Ji Wei Wu
    Dezhou University, China
    Appl Microbiol Biotechnol 92:253-62. 2011
    Bacterial extracellular metalloproteases (BEMPs) are a large group of metal-containing proteases secreted by heterotrophic bacteria...
  77. pmc A rapid and sensitive fluorometric method for the quantitative analysis of snake venom metalloproteases and their inhibitors
    J E Biardi
    Department of Biology, Fairfield University, 1073 North Benson Road, Fairfield, CT 06824, USA
    Toxicon 57:342-7. 2011
    b>Metalloproteases are responsible for the hemorrhagic effects of many snake venoms and contribute to other pathways that lead to local tissue damage...
  78. ncbi Rock squirrel (Spermophilus variegatus) blood sera affects proteolytic and hemolytic activities of rattlesnake venoms
    James E Biardi
    Department of Biology, Fairfield University, 1073 North Benson Road, Fairfield, CT 06824, USA
    Toxicon 57:323-31. 2011
    ..These results suggest that rock squirrels (S. variegatus) can defend against metalloproteases and other proteases after envenomation from at least two of five rattlesnake predators they might encounter...
  79. ncbi Contribution of metalloproteases, serine proteases and phospholipases A2 to the inflammatory reaction induced by Bothrops jararaca crude venom in mice
    Bianca Cestari Zychar
    Laboratorio de Fisiopatologia, Instituto Butantan, Av Vital Brazil, 1500, 05503 900 Sao Paulo, SP, Brazil
    Toxicon 55:227-34. 2010
    ..Notwithstanding, the relative participation of serine proteases, metalloproteases and phospholipases A(2) in the inflammatory reaction produced by crude Bothrops venoms is poorly understood...
  80. ncbi Hydrated conidia of Metarhizium anisopliae release a family of metalloproteases
    Sohail S Qazi
    Microbial Biotechnology Molecular Microbiology Laboratories, Department of Applied Microbiology and Food Science, University of Saskatchewan, Saskatoon, Canada
    J Invertebr Pathol 95:48-59. 2007
    ..anisopliae in its saprobic or pathogenic modalities. To our knowledge this is the first report describing release of metalloprotease isozymes from conidia...
  81. ncbi Tackling EGFR signaling with TACE antagonists: a rational target for metalloprotease inhibitors in cancer
    Paraic A Kenny
    Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, MS977 225A, University of California, Berkeley, CA 94720, USA
    Expert Opin Ther Targets 11:1287-98. 2007
    ..This review presents the background to these findings and a rationale for the continued development of TACE inhibitors for the treatment of epidermal growth factor receptor-dependent epithelial tumors...
  82. ncbi The zinc metallopeptidase family: new faces, new functions
    Rebecca A Lew
    Department of Biochemistry, Molecular Biology, Monash University, Clayton Victoria, Australia 3800
    Protein Pept Lett 11:407-14. 2004
  83. ncbi Roles of metalloproteases in metastatic niche
    N Rucci
    Department of Experimental Medicine, University of L Aquila, L Aquila, Italy
    Curr Mol Med 11:609-22. 2011
    ....
  84. ncbi Structural aspects of the metzincin clan of metalloendopeptidases
    F Xavier Gomis-Ruth
    Institut de Biologia Molecular de Barcelona, CID CSICC Jordi Girona, 18 26 08034 Barcelona, Spain
    Mol Biotechnol 24:157-202. 2003
    ..Moreover, newly available genomic sequences have unveiled novel putative metzincin families and new hypothetical members of existing ones...
  85. ncbi Metalloproteases and egg-hatching in Pediculus humanus, the body (clothes) louse of humans (Phthiraptera: Insecta)
    V M Bowles
    Centre for Animal Biotechnology, School of Veterinary Science, The University of Melbourne, Melbourne, 3010, Australia
    Parasitology 135:125-30. 2008
    ..05) louse egg-hatch (100% and 58%, respectively). The presence of metalloproteases at the time of egg-hatch and the inhibition of egg-hatch in P...
  86. pmc Impaired autoproteolytic cleavage of mCLCA6, a murine integral membrane protein expressed in enterocytes, leads to cleavage at the plasma membrane instead of the endoplasmic reticulum
    Melanie K Bothe
    Department of Veterinary Pathology, Freie Universitaet Berlin, 14163 Berlin, Germany
    Mol Cells 33:251-7. 2012
    ..They have been shown to modulate endogenous chloride conductance, possibly by acting as metalloproteases. Based on the differential processing of the subunits after posttranslational cleavage, two subgroups of CLCA ..
  87. ncbi Presence of peptide inhibitors in rattlesnake venoms and their effects on endogenous metalloproteases
    Sean M Munekiyo
    Department of Biological Sciences, University of Northern Colorado, 501 20th St, CB 92, Greeley, CO 80639 0017, USA
    Toxicon 45:255-63. 2005
    ..of rattlesnakes and evaluated the role of these peptides in inhibiting and stabilizing isolated major venom metalloproteases (Cvo Pr V and cromipyrrhin) from Crotalus oreganus oreganus and C. mitchelli pyrrhus venom...
  88. ncbi Metalloproteases and the degradome
    Alejandro P Ugalde
    Departamento de Bioquimica y Biologia Molecular, Universidad de Oviedo, Oviedo, Spain
    Methods Mol Biol 622:3-29. 2010
    b>Metalloproteases comprise a heterogeneous group of proteolytic enzymes whose main characteristic is the utilization of a metal ion to polarize a water molecule and perform hydrolytic reactions...
  89. ncbi Papilin, a novel component of basement membranes, in relation to ADAMTS metalloproteases and ECM development
    John H Fessler
    Department of Molecular, Cell and Developmental Biology, Molecular Biology Institute, University of California at Los Angeles, Los Angeles, CA 90095 1570, USA
    Int J Biochem Cell Biol 36:1079-84. 2004
    ..with a cassette of protein domains at the carboxyl-end of the ADAMTS subgroup of secreted, matrix-associated metalloproteases. Papilins primarily occur in basement membranes...
  90. ncbi Role of metalloproteases in retinal degeneration induced by violet and blue light
    C Sánchez-Ramos
    University Complutense de Madrid, Madrid, Spain
    Adv Exp Med Biol 664:159-64. 2010
    An essential role for metalloproteases (MMPs) has been described in blood vessel neoformation and the removal of cell debris. MMPs also play a key role in degenerative processes and in tumors...
  91. pmc Characterization of the astacin family of metalloproteases in C. elegans
    Ja On Park
    Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada
    BMC Dev Biol 10:14. 2010
    Astacins are a large family of zinc metalloproteases found in bacteria and animals. They have diverse roles ranging from digestion of food to processing of extracellular matrix components. The C...
  92. pmc Spatial localization of bacteria controls coagulation of human blood by 'quorum acting'
    Christian J Kastrup
    Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA
    Nat Chem Biol 4:742-50. 2008
    ..We refer to this mechanism as 'quorum acting' to distinguish it from quorum sensing--it does not require a change in gene expression, it can be rapid and it can be independent of bacterium-to-bacterium communication...
  93. pmc Activation of latent myostatin by the BMP-1/tolloid family of metalloproteinases
    Neil M Wolfman
    Department of Inflammation, Wyeth Research, 200 CambridgePark Drive, Cambridge, MA 02140, USA
    Proc Natl Acad Sci U S A 100:15842-6. 2003
    ....
  94. ncbi Astacin-like metalloproteases are a gene family of toxins present in the venom of different species of the brown spider (genus Loxosceles)
    Dilza Trevisan-Silva
    Department of Cell Biology, Federal University of Parana, Brazil
    Biochimie 92:21-32. 2010
    ..Moreover, mRNAs extracted from L. laeta and L. gaucho venom glands were screened for astacin-like metalloproteases, and cDNAs obtained using LALP1-specific primers were sequenced, and their deduced amino acid sequences ..
  95. pmc The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice
    Damiana Chiavolini
    Dipartimento di Biologia Molecolare, Laboratorio di Microbiologia Molecolare e Biotecnologia, Universita di Siena, Siena, Italy
    BMC Microbiol 3:14. 2003
    ....
  96. ncbi Hax1-mediated processing of HtrA2 by Parl allows survival of lymphocytes and neurons
    Jyh Rong Chao
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Nature 452:98-102. 2008
    ..Together, the results identify a previously unknown sequence of interactions involving a Bcl-2-family-related protein and mitochondrial proteases in the ability to resist the induction of apoptosis when cytokines are limiting...
  97. ncbi A novel P-I class metalloproteinase with broad substrate-cleaving activity, agkislysin, from Agkistrodon acutus venom
    Wen Jeng Wang
    Chang Gung Institute of Technology, Kwei Shan, Tao Yuan 33303, Taiwan, ROC
    Biochem Biophys Res Commun 324:224-30. 2004
    ..These findings suggest that agkislysin is a P-I class metalloproteinase with unique biological properties...
  98. ncbi Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulfide bond formation and their contribution to venom complexity
    Jay W Fox
    Department of Microbiology, University of Virginia, Charlottesville, VA 22908 0734, USA
    FEBS J 275:3016-30. 2008
    ....
  99. ncbi High resolution analysis of snake venom metalloproteinase (SVMP) peptide bond cleavage specificity using proteome based peptide libraries and mass spectrometry
    Adriana F Paes Leme
    Brazilian Biosciences National Laboratory, CNPEM, Campinas, Brazil
    J Proteomics 74:401-10. 2011
    ....
  100. ncbi Interaction of the cysteine-rich domain of snake venom metalloproteinases with the A1 domain of von Willebrand factor promotes site-specific proteolysis of von Willebrand factor and inhibition of von Willebrand factor-mediated platelet aggregation
    Solange M T Serrano
    Laboratório Especial de Toxinologia Aplicada and CAT CEPID, Instituto Butantan, Sao Paulo, Brazil
    FEBS J 274:3611-21. 2007
    ..Thus, specific interaction of cysteine-rich domain-containing SVMPs with VWF may function to promote the hemorrhage caused by SVMP proteolysis of capillary basements and surrounding stromal extracellular matrix...
  101. ncbi Shedding of the amyloid precursor protein-like protein APLP2 by disintegrin-metalloproteinases
    Kristina Endres
    Institute of Biochemistry, Johannes Gutenberg University Mainz, Germany
    FEBS J 272:5808-20. 2005
    ..Retinoic acid treatment of two neuroblastoma cell lines upregulated the expression of both APLP2 and ADAM10, thus leading to an increased release of soluble APLP2...

Research Grants64

  1. Discovery metabolite profiling of the prolyl peptidases
    Alan Saghatelian; Fiscal Year: 2009
    ..Furthermore, the application of DMP to peptidases will demonstrate the generality of this approach for the future characterization of medically relevant enzymes and signaling pathways. ..
  2. Regulation of Astrocyte TIMP-1 in HIV-Associated Dementia
    JEREL ADAM FIELDS; Fiscal Year: 2011
    ..The goal of this proposal is to characterize regulation of primary human astrocyte tissue inhibitors of metalloproteases (TIMP) expression during Human Immunodeficiency Virus (HIV)-associated dementia (HAD)...
  3. PARP inhibitor and Redox Catalyst Conjugate for Traumatic Brain Injury
    Kanneganti Murthy; Fiscal Year: 2012
    ..of inflammatory cytokines, chemokines, adhesion molecules and inflammatory mediators, including matrix metalloproteases. Despite the apparent centrality of PARP activation to the initiation and recovery phases of TBI, there are ..
  4. The Regulation of Salivary Gland Regeneration by P2Y2 Nucleotide Receptors
    GARY ANDREW WEISMAN; Fiscal Year: 2013
    ..known to regulate cell growth and differentiation involving integrins, growth factor receptors, matrix metalloproteases, adhesion molecules and cytoskeletal proteins...
  5. PHYSIOLOGY AND CLONING OF B FRAGILIS ENTEROTOXIN
    CYNTHIA SEARS; Fiscal Year: 2010
    ..fragilis toxin (BFT). By cell-surface proteolysis, matrix metalloproteases regulate eukaryotic cell signal transduction and function including cell proliferation, tumor progression and ..
  6. Fast accurate ultrasensitive in vitro assay to determine BoNT potency &stability
    ARMIN H REITMAIR; Fiscal Year: 2012
    ..BoNTs are zinc metalloproteases that cleave and inactivate proteins involved in synaptic vesicle fusion and acetylcholine release...
  7. High-throughput multiplex microsphere screening for toxin protease inhibitors
    Steven W Graves; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): The zinc metalloproteases from Botulinum neurotoxin (BoNT) and the Lethal Toxin (LT) of B. anthracis are critical for the toxicity of their host organisms and are excellent pharmaceutical targets...
  8. A New Pathway for Neutrophil-induced Airway Inflammation
    J Edwin Blalock; Fiscal Year: 2013
    ..The degradation of collagen and release of PGP is shown to be a stepwise process initially involving matrix metalloproteases (MMP)-8 and/or 9 with prolyl endopeptidase (PE) catalyzing the final reaction...
  9. Effect of Macrolide Treatment on a Novel Pathway of Neutrophilic Inflammation in
    J Edwin Blalock; Fiscal Year: 2010
    ..The enzymatic production of PGP is a stepwise process initially involving matrix metalloproteases (MMP)-8 and/or MMP-9 with prolyl endopeptidase (PE), a serine protease, catalyzing the final reaction...
  10. Fluorescence enhanced optical tomography
    Eva M Sevick-Muraca; Fiscal Year: 2012
    ..a peptide that (i) is dual-labeled with 64Cu-DOTA and Li-Cor's IRDye800CW and (ii) targets active matrix metalloproteases, MMP-2, and -9 in gated PET, CT, and NIR optical tomography studies of myocardial infarct and of lung cancer ..
  11. Systems microscopy analysis of tumor cell motility in microenvironment context
    Bojana Gligorijevic; Fiscal Year: 2013
    ..Here, tumor cells become invasive and degrade extracellular matrix, mainly by matrix metalloproteases (MMPs)...
  12. Role of Meprins in Ischemia Reperfusion induced renal injury
    Elimelda Moige Ongeri; Fiscal Year: 2013
    ..The cellular mechanisms underlying IR renal injury are not known. Meprins, metalloproteases that are abundantly expressed in the brush border membranes (BBM) of proximal kidney tubules, have been ..
  13. Alcohol Abuse and HIV-1: Mechanisms of Combined CNS Injury and Interventions
    Yuri Persidsky; Fiscal Year: 2012
    ..of pro-inflammatory factors (via activation of Src kinases and phospholipase A2);and (3) enhanced activity of metalloproteases (MMPs) resulting in loss of blood brain barrier (BBB) integrity...
  14. Novel virulence and exoenzyme regulators
    ROBERT M SHANKS; Fiscal Year: 2013
    ..Test the hypothesis that EepR/S, four metalloproteases and the ShlA hemolysin are required for S. marcescens cytotoxicity in vitro and ocular pathogenesis in vivo...
  15. Reversing Tissue Fibrosis to Improve Immune Reconstitution in HIV
    Timothy W Schacker; Fiscal Year: 2013
    ..Importantly, and in distinction to pirfenidone, losartan also inhibits matrix metalloproteases that stimulate collagen production and has anti-inflammatory properties that may IA in a broader context...
  16. A novel proteolytic system of pulmonary inflammation
    Amit Gaggar; Fiscal Year: 2013
    ..The PI has shown that PGP is released from collagen by a stepwise process initially involving matrix metalloproteases (MMP)-8 and/or 9 with prolyl endopeptidase (PE) catalyzing the final reaction...
  17. Small-molecule inhibitors of hADAM12
    Jens Berthelsen; Fiscal Year: 2012
    ..are expressed as pro-proteins, which are recognized, cleaved and thereby activated by membrane-anchored metalloproteases (sheddases) such as a disintegrin and metalloproteases (ADAM's)...
  18. VERTEBRATE PRIMARY MOUTH FORMATION
    Hazel L Sive; Fiscal Year: 2013
    ..Focus is on the role of matrix metalloproteases (MMPs) that degrade the basement membrane...
  19. Intramembrane-Cleaving metalloproteases of Bacillus subtilis
    LEE R KROOS; Fiscal Year: 2013
    ..In addition to expanding knowledge of RasP, the approach could be used to identify substrates of other MIPs, overcoming a critical barrier to progress in the field. ..
  20. Protease-activated receptors in vascular integrity and dysfunction
    Athan Kuliopulos; Fiscal Year: 2010
    Emerging evidence suggests that matrix metalloproteases (MMPs) play a pivotal role in vascular integrity and wound healing, however, inappropriate MMP activity may underlie the pathogenesis of several diseases including restenosis, ..
  21. Arbovirus midgut escape mechanisms
    A Lorena Passarelli; Fiscal Year: 2013
    ..Baculoviruses use an elegant mechanism that signals a stepwise cascade of protease activation, wherein matrix metalloproteases become activated and in turn activate effector caspases, which directly cleave components of the basal lamina...
  22. EPCR, TAFI as Regulators of PMN/Endothelial Interaction
    Florea Lupu; Fiscal Year: 2009
    ..of protein C and TAFI by the TM/thrombin complex and through release of soluble EPCR by endothelial-derived metalloproteases. We postulate that TAFla (procarboxypeptidase beta) attenuates neutrophil activation by inactivating C5a, and ..
  23. 2010 Proprotein Processing, Trafficking, and Secretion;Gordon Research Conferenc
    Lloyd D Fricker; Fiscal Year: 2013
    ..a foundation to study the role of additional membrane-associated proteolytic systems, particularly the matrix metalloproteases and the secretases, that control tissue remodeling and membrane shedding in normal cells, as well as the ..
  24. Novel Mechanisms of Chronic Inflammation in Sjogren's Syndrome
    GARY ANDREW WEISMAN; Fiscal Year: 2010
    ..to salivary gland cells, including the contribution of growth factor receptor transactivation and matrix metalloproteases (Specific Aim 2)...
  25. Complement protein synthesis and activation by human mast cells
    Yoshihiro Fukuoka; Fiscal Year: 2010
    ..Specific Aim 2 will analyze which matrix metalloproteases activate complement on human mast cells...
  26. The role of metalloprotease-PAR1 signaling in vascular injury and repair
    KARYN MARIE AUSTIN; Fiscal Year: 2013
    ..Matrix metalloproteases (MMPs) are a family of endopeptidases that are largely responsible for the degradation of extracellular ..
  27. A Multidisciplinary Analysis of Gelsolin Amyloid Disease
    Jeffery W Kelly; Fiscal Year: 2010
    ..Once outside the cell, the 68 kDa fragment is cleaved again by specific matrix metalloproteases (MMPs) including MT1-MMP (MMP14) affording 5 and 8 kDa fragments...
  28. CONTROL OF DRUG AND ETHANOL METABOLISM
    Gavin E Arteel; Fiscal Year: 2010
    ..are: a) PAI-1-/- mice are protected against hepatic fibrosis at the level of matrix resolution, b) Matrix metalloproteases are required for enhanced matrix resolution in PAI-1-/- mice, c) Inhibition of PAI-1 will enhance recovery ..
  29. Mechanism of Cranial Neural Crest Cell Migration
    DOMINIQUE R ALFANDARI; Fiscal Year: 2013
    ..Among these proteins the ADAM cell surface metalloproteases can regulate both cell signaling and cell adhesion...
  30. Regulation of Cell-Cell Interactions by Matrix Metalloproteases
    Dirk Bohmann; Fiscal Year: 2013
    ..These experiments will provide insight into the structure-function relationships in cell surface bound matrix metalloproteases and may yield general information on the mechanisms governing the regulatory specificity of MMPs...
  31. Matrix Metalloproteinases and Cocaine
    Barbara A Sorg; Fiscal Year: 2012
    ..These studies will have a positive impact on the drug abuse field because they will determine the potential for using MMP inhibitors to disrupt reconsolidation of cocaine memories that may underlie chronic relapse. ..
  32. Crosstalk between Desmogleins and Canonical Hedgehog Signaling
    Donna Brennan; Fiscal Year: 2013
    ..Gli activity, by both determining at what level of Hh signaling Dsg2 is acting and if Dsg2 processing by metalloproteases and/or caspases is necessary for this regulation...
  33. Protein-Protein Interactions in the Biology of Beta-APP
    Dennis J Selkoe; Fiscal Year: 2012
    ..have fundamental implications for peptide turnover in the mammalian brain and for the cell biology of metalloproteases. Many scientists now support the idea that the gradual buildup of a small protein, amyloid beta-protein (..
  34. CD147 as a Novel Target in Head and Neck Cancer
    Eben L Rosenthal; Fiscal Year: 2013
    ..Although CD147 is known to stimulate fibroblasts to produce matrix metalloproteases (MMPs) and vascular endothelial growth factor (VEGF), we have identified upregulation and secretion of ..
  35. Prolyl endopeptidase, a novel protease important in lung inflammation
    PHILIP JAMES O'REILLY; Fiscal Year: 2013
    ..The enzymatic production of PGP is a stepwise process initially involving matrix metalloproteases with prolyl endopeptidase (PE) catalyzing the final reaction...
  36. The Role of BRAF Mutation in Thyroid Cancer Invasion
    Sareh Parangi; Fiscal Year: 2013
    ..adhesion molecules including Thrombospondin-1, integrins and other stromally active proteases, such as matrix metalloproteases. Here we posit that BRAF mutation and changes in these adhesion molecules plays a crucial role in the ..
  37. Inhibitors of BMP-1/TLD proteases as novel therapeutics for muscular dystrophy
    Se Jin Lee; Fiscal Year: 2010
    ..A variety of biochemical and genetic studies have demonstrated that members of the BMP-1/tolloid family of metalloproteases play a critical role in regulating MSTN latency by cleaving and thereby inactivating the propeptide...
  38. Biological roles of ADAMTS Proteases
    SUNEEL APTE; Fiscal Year: 2009
    Abstract ADAMTS metalloproteases are prominent in arthritis as aggrecan-degrading enzymes (aggrecanases), with ADAMTS5 being a major target for drug development...
  39. Molecular Analysis of the Rip1 Virulence Pathway of M. tuberculosis
    MICHAEL STEPHEN GLICKMAN; Fiscal Year: 2010
    ..The Rip1 (Rv2869c) intramembrane protease is a member of the Site two protease (S2P) class of intramembrane metalloproteases which cleave substrate proteins within transmembrane domains...
  40. Role of Fibulin-1 in APP Processing
    Mark S Kindy; Fiscal Year: 2013
    ..of amyloid precursor protein (APP), which is mediated by several members of the disintegrin family of metalloproteases, ADAM9, ADAM10 and ADAM17...
  41. Using Dendrimers to Design Multivalent Therapeutic Agents
    Mary J Cloninger; Fiscal Year: 2013
    ..The synthesis of dendrimers bearing peptide substrates for matrix metalloproteases is also proposed...
  42. Characterization of a novel regulator of cell migration
    ERIN JEAN CRAM; Fiscal Year: 2012
    ..The genes controlling DTC migration, including metalloproteases, integrins, and Rac GTPases, are strikingly similar to the genes required during metastasis...
  43. Tumor Stromal Interactions in Head and Neck Cancer
    Eben Rosenthal; Fiscal Year: 2009
    ..promotes local tumor cell invasion in tumors of the head and neck by inducing the expression of matrix metalloproteases (MMPs)...
  44. Gordon Research Conference and Gordon-Kenan Research Seminar on Matrix Metallopro
    RAFAEL A FRIDMAN; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): The Gordon Research Conference (GRC) on Matrix Metalloproteases (MMP) is designed to provide scientists and physicians from a wide range of disciplines with a forum to report the latest research ..
  45. Identification of small molecule furin-like protease inhibitors
    JULIA COPPOLA; Fiscal Year: 2009
    ..immature latent proteins, including growth factors and hormones, receptors, plasma proteins, and matrix metalloproteases containing a specific recognition cleavage motif (RX(K/R)R?), to their mature or functional forms...
  46. Proteolysis in silico: statistics, structural chemistry, and biology
    Piotr Cieplak; Fiscal Year: 2013
    ..However, our current effort will focus mainly on matrix metalloproteases (MMPs), caspases, and several protein convertases (PCs) belonging to the serine protease family because a ..
  47. Ribosomal Synthesis of Peptides with Unnatural Building Blocks
    Peter G Schultz; Fiscal Year: 2013
    ..g., serine proteases, metalloproteases, etc.) 2...
  48. Blood-brain barrier function in epilepsy: new targets for therapy
    Bjoern Bauer; Fiscal Year: 2013
    ..We will determine expression of tight junction proteins and matrix metalloproteases, and assess barrier leakage...
  49. ADAM12 in Breast Tumor Initiating Cells
    Anna Zolkiewska; Fiscal Year: 2013
    ..objective of this application is to evaluate ADAM12, a member of the ADAM family of cell-surface disintegrin-metalloproteases, as a novel marker and a novel therapeutic target in BTICs...
  50. Anaplasma - B. burgorferi/endothelijm interactions
    DENNIS GRAB; Fiscal Year: 2006
    ..burgdorferi binding and passage through endothelial cells is in part mediated by host matrix metalloproteases (MMPs), and that coincubations of B. burgdorferi and A...
  51. Subcellular Distribution &Regulation of Ala1 Adrenoceptors
    Debra A Schwinn; Fiscal Year: 2013
    ..with aiaARs with higher affinity than Alpha1bARs We also plan to extend our studies by studying matnx metalloproteases (MMPs) in mediating Alpha1aAR signaling via EGFR transactivation compared with Alpha1bARs...
  52. INTRA-ARTICULAR DELIVERY OF PEPTIDES AND PROTEINS
    Paul Simpson; Fiscal Year: 1999
    ..Release can be in response to matrix metalloproteases which are elevated in inflamed or diseased joints...
  53. 800 MHz Spectrometer for Biomolecular NMR in Missouri
    STEVEN VAN DOREN; Fiscal Year: 2006
    ..The Van Doren group will investigate (i) interactions of metalloproteases with inhibitor and substrate proteins and (ii) interactions of transforming tumor viral proteins with their ..
  54. HEREDITARY SPASTIC PARAPLEGIA--CLINICAL, HISTOCHEMICAL,
    John Fink; Fiscal Year: 2001
    ..We present in Preliminary Data, for example, that paraplegin is homologous to yeast mitochondrial metalloproteases AFG3, RCA1, and YME1 and that abnormal mitochondrial histochemistry is evident in muscle biopsy in patients ..
  55. Treatment of Anthrax with Nonantimicrobial Tetracyclines
    Sanford Simon; Fiscal Year: 2003
    ..The CMTs are inhibitors of zinc matrix metalloproteases (MMPs), but they also can inhibit zinc metalloproteases of bacterial origin...
  56. Matrix Metalloproteases Regulate Mesenchymal Stem Cells
    Zena Werb; Fiscal Year: 2006
    ..These studies will have direct implications for the treatment of skeletal injuries resulting from disease or malformation, and for injuries that are refractory to conventional therapy. ..
  57. Mechanisms of HAART-Induced Endothelial Dysfunction
    Krishna Agrawal; Fiscal Year: 2006
    ..Expression of cell adhesion molecules (V-CAM, I-CAM and E-selectin), and activation of metalloproteases (MMPs) in ECs will be determined by RTPCR and ELISA, and zymography, respectively...
  58. Eosinophil-Airway Epithelial Fas-FasL Interactions
    Kimm Hamann; Fiscal Year: 2004
    ..of Fas ligand (FasL) in human airway epithelial cells and the role of matrix (MMP) and disintegrin (ADAM) metalloproteases in the release of soluble FasL (sFasL) from these cells...
  59. ECM PROTEASES IN INFLAMMATION, FIBROSIS & TUMORIGENESIS
    Gregory Goldberg; Fiscal Year: 2000
    DESCRIPTION: Secreted metalloproteases play a leading role in processes of tissue remodeling by initiating the degradation of extracellular matrix (ECM)...
  60. MMP1 & MMP9: Mechanism of Activation & Substrate Binding
    Gregory Goldberg; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Metalloproteases (MMPs) play a pivotal role in tissue remodeling during morphogenesis, wound healing, angiogenesis, uterine involution and bone resorption...
  61. Zinc metalloprotease families in Trypansoma brucei
    JOHN DONELSON; Fiscal Year: 2009
    ..The biological properties and differential expression of related groups of zinc metalloproteases in the African trypanosome Trypanosoma brucei will be studied using the techniques of traditional gene ..
  62. Fibrogenic factors in ocular cicatricial pemphigoid
    A Ahmed; Fiscal Year: 2004
    ..role for macrophage colony-stimulating factor (m-CSF), transforming growth factor (TGF)-beta1, matrix metalloproteases (MMPs), and tissue inhibitors of metalloproteases (TIMPs) in regulating inflammatory and fibrotic events in ..
  63. Neuropeptidase Function
    David Rodgers; Fiscal Year: 2007
    ..Knowledge of these enzymes will allow us to manipulate their activity in order to treat diseases of the nervous system as well as other disorders ..
  64. A Novel & Tailorable Topical Wound Therapy Based on a Semi-Interpenetrating Polym
    DAVID VACHON; Fiscal Year: 2007
    ..with unique properties and processing characteristics has been proven effective against multiple serine and metalloproteases in testing...