carboxypeptidase b

Summary

Summary: A ZINC-dependent carboxypeptidase primary found in the DIGESTIVE SYSTEM. The enzyme catalyzes the preferential cleavage of a C-terminal peptidyl-L-lysine or arginine. It was formerly classified as EC 3.4.2.2 and EC 3.4.12.3.

Top Publications

  1. ncbi Regulation of plasminogen binding to neutrophils
    T Herren
    Joseph J. Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, The Cleveland Clinic Foundation, Cleveland, OH, USA
    Blood 97:1070-8. 2001
  2. pmc Regulation of chemerin bioactivity by plasma carboxypeptidase N, carboxypeptidase B (activated thrombin-activable fibrinolysis inhibitor), and platelets
    Xiao Yan Du
    Division of Hematology, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Biol Chem 284:751-8. 2009
  3. ncbi Active carboxypeptidase B is present in free form in serum from patients with acute pancreatitis
    Anders Borgstrom
    Department of Surgery, Malmo University Hospital, Sweden
    Pancreatology 5:530-6. 2005
  4. ncbi Expression of human brain carboxypeptidase B, a possible cleaving enzyme for beta-amyloid precursor protein, in peripheral fluids
    A Matsumoto
    Department of Radiation Biophysics and Genetics, Kobe University School of Medicine, Kusunoki cho chuo ki 7 5 1, Kobe650 0017, Japan
    Neurosci Res 39:313-7. 2001
  5. ncbi The NMR structure and dynamics of the two-domain tick carboxypeptidase inhibitor reveal flexibility in its free form and stiffness upon binding to human carboxypeptidase B
    David Pantoja-Uceda
    Instituto de Quimica Fisica Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain
    Biochemistry 47:7066-78. 2008
  6. pmc Thrombin-activatable procarboxypeptidase B regulates activated complement C5a in vivo
    Toshihiko Nishimura
    Department of Medicine, Stanford University School of Medicine and Veterans Administration Palo Alto Health Care System, Palo Alto, CA 94304, USA
    Blood 109:1992-7. 2007
  7. pmc Regulation of tissue inflammation by thrombin-activatable carboxypeptidase B (or TAFI)
    Lawrence L K Leung
    Department of Medicine, Stanford University School of Medicine and Veterans Administration Palo Alto Health Care System, Palo Alto, CA 94304, USA
    Mol Immunol 45:4080-3. 2008
  8. ncbi Monocyte chemoattractant protein 1, active carboxypeptidase B and CAPAP at hospital admission are predictive markers for severe acute pancreatitis
    S Regner
    Department of Surgery, Malmo University Hospital, Lund University, Lund, Sweden
    Pancreatology 8:42-9. 2008
  9. ncbi On the mechanism of the antifibrinolytic activity of plasma carboxypeptidase B
    D V Sakharov
    Gaubius Laboratory, TNO Prevention and Health, 2301 CE Leiden, The Netherlands
    J Biol Chem 272:14477-82. 1997
  10. ncbi Activated human plasma carboxypeptidase B is retained in the blood by binding to alpha2-macroglobulin and pregnancy zone protein
    Z Valnickova
    Duke University Medical Center, Department of Pathology, Durham, North Carolina 27710, USA
    J Biol Chem 271:12937-43. 1996

Research Grants

  1. Cross-talk Between Coagulation, Inflammation, and Immunity
    Lawrence L Leung; Fiscal Year: 2013
  2. The Role of Carboxypeptidase B and its Substrates in Rheumatoid Arthritis
    William H Robinson; Fiscal Year: 2013
  3. ENZYMES INVOLVED IN DYNORPHIN BIOSYNTHESIS
    Lakshmi Devi; Fiscal Year: 1993
  4. STRUCTURE AND FUNCTION OF PANCREATIC ENZYMES
    C Hirs; Fiscal Year: 1980
  5. CELL BIOLOGY OF CARBOXYPEPTIDASE D
    Lloyd Fricker; Fiscal Year: 2003
  6. Function of Carboxypeptidase D
    Lloyd Fricker; Fiscal Year: 2008
  7. Quantitative Peptidomics of Food Deprivation
    Lloyd Fricker; Fiscal Year: 2005
  8. REGULATION OF NEUROPEPTIDE PROCESSING ENZYMES
    Lloyd Fricker; Fiscal Year: 2002
  9. Direct Cellular Effects of Blood Coagulation Proteases
    LAURENT MOSNIER; Fiscal Year: 2007
  10. Peptidomics of Cocaine and Amphetamine Abuse
    Lloyd Fricker; Fiscal Year: 2005

Detail Information

Publications205 found, 100 shown here

  1. ncbi Regulation of plasminogen binding to neutrophils
    T Herren
    Joseph J. Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, The Cleveland Clinic Foundation, Cleveland, OH, USA
    Blood 97:1070-8. 2001
    ..This increase was abolished by soybean trypsin inhibitor and was susceptible to carboxypeptidase B treatment, implicating proteolysis and exposure of carboxy-terminal lysines in the enhanced interaction...
  2. pmc Regulation of chemerin bioactivity by plasma carboxypeptidase N, carboxypeptidase B (activated thrombin-activable fibrinolysis inhibitor), and platelets
    Xiao Yan Du
    Division of Hematology, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Biol Chem 284:751-8. 2009
    ....
  3. ncbi Active carboxypeptidase B is present in free form in serum from patients with acute pancreatitis
    Anders Borgstrom
    Department of Surgery, Malmo University Hospital, Sweden
    Pancreatology 5:530-6. 2005
    Active carboxypeptidase B (aCAP) is the main product of activation of procarboxypeptidase...
  4. ncbi Expression of human brain carboxypeptidase B, a possible cleaving enzyme for beta-amyloid precursor protein, in peripheral fluids
    A Matsumoto
    Department of Radiation Biophysics and Genetics, Kobe University School of Medicine, Kusunoki cho chuo ki 7 5 1, Kobe650 0017, Japan
    Neurosci Res 39:313-7. 2001
    Human brain carboxypeptidase B (HBCPB) is a novel brain protease that processes native brain beta-amyloid precursor protein (APP) in vitro...
  5. ncbi The NMR structure and dynamics of the two-domain tick carboxypeptidase inhibitor reveal flexibility in its free form and stiffness upon binding to human carboxypeptidase B
    David Pantoja-Uceda
    Instituto de Quimica Fisica Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain
    Biochemistry 47:7066-78. 2008
    ..of the tick carboxypeptidase inhibitor (TCI) and its backbone dynamics, free and in complex with human carboxypeptidase B, have been determined by NMR spectroscopy...
  6. pmc Thrombin-activatable procarboxypeptidase B regulates activated complement C5a in vivo
    Toshihiko Nishimura
    Department of Medicine, Stanford University School of Medicine and Veterans Administration Palo Alto Health Care System, Palo Alto, CA 94304, USA
    Blood 109:1992-7. 2007
    ..Its activation, along with protein C, by the endothelial thrombin-TM complex represents a homeostatic response to counteract the inflammatory mediators generated at the site of vascular injury...
  7. pmc Regulation of tissue inflammation by thrombin-activatable carboxypeptidase B (or TAFI)
    Lawrence L K Leung
    Department of Medicine, Stanford University School of Medicine and Veterans Administration Palo Alto Health Care System, Palo Alto, CA 94304, USA
    Mol Immunol 45:4080-3. 2008
    ..Along with the activation of protein C, the activation of proCPB by the endothelial thrombin-thrombomodulin complex represents a homeostatic feedback mechanism in regulating thrombin's pro-inflammatory functions in vivo...
  8. ncbi Monocyte chemoattractant protein 1, active carboxypeptidase B and CAPAP at hospital admission are predictive markers for severe acute pancreatitis
    S Regner
    Department of Surgery, Malmo University Hospital, Lund University, Lund, Sweden
    Pancreatology 8:42-9. 2008
    ..Monocyte chemotactic protein-1 (MCP-1) is an early inflammatory marker and increases before complications in severe AP. We conducted a cohort study to evaluate these markers as predictors for severe AP...
  9. ncbi On the mechanism of the antifibrinolytic activity of plasma carboxypeptidase B
    D V Sakharov
    Gaubius Laboratory, TNO Prevention and Health, 2301 CE Leiden, The Netherlands
    J Biol Chem 272:14477-82. 1997
    The precursor of plasma carboxypeptidase B (pCPB) also known as thrombin-activable fibrinolysis inhibitor can be converted by thrombin to an active enzyme capable of eliminating C-terminal Lys- and Arg-residues from proteins...
  10. ncbi Activated human plasma carboxypeptidase B is retained in the blood by binding to alpha2-macroglobulin and pregnancy zone protein
    Z Valnickova
    Duke University Medical Center, Department of Pathology, Durham, North Carolina 27710, USA
    J Biol Chem 271:12937-43. 1996
    A 66-kDa glycosylated carboxypeptidase, plasma pro-carboxypeptidase B (pro-plasma CPB), has recently been identified in human blood (Eaton, D. L., Malloy, B. E., Tsai, S. P., Henzel, W., and Drayna, D. (1991) J. Biol. Chem...
  11. ncbi cpbAg1 encodes an active carboxypeptidase B expressed in the midgut of Anopheles gambiae
    C Lavazec
    Unité de Biologie et Génétique du Paludisme, Institut Pasteur, Paris, France
    Insect Mol Biol 14:163-74. 2005
    ..Here, we report the characterization of one of these genes, cpbAg1, which codes for the first zinc-carboxypeptidase B identified in An. gambiae and in any insect...
  12. ncbi Isolation, molecular cloning, and partial characterization of a novel carboxypeptidase B from human plasma
    D L Eaton
    Department of Cardiovascular Research, Genentech, Inc, South San Francisco, California 94080
    J Biol Chem 266:21833-8. 1991
    ..When activated by trypsin, it hydrolyzes carboxypeptidase B substrates, hippuryl-Arg and hippuryl-Lys, but not carboxypeptidase A substrates, and it is inhibited by the ..
  13. ncbi Regulation of tissue inflammation by thrombin-activatable carboxypeptidase B (or TAFI)
    Lawrence L K Leung
    Department of Medicine, Division of Hematology, Stanford University School of Medicine and Veterans Administration Palo Alto Health Care System, Stanford, CA 94305, USA
    Adv Exp Med Biol 632:61-9. 2008
    ..Along with the activation of protein C, the activation of proCPB by the endothelial thrombin-thrombomodulin complex represents a homeostatic feedback mechanism in regulating thrombin's pro-inflammatory functions in vivo...
  14. ncbi Regulation of fibrinolysis by thrombin activatable fibrinolysis inhibitor, an unstable carboxypeptidase B that unites the pathways of coagulation and fibrinolysis
    Laurent O Mosnier
    The Scripps Research Institute, Department of Molecular and Experimental Medicine, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Arterioscler Thromb Vasc Biol 26:2445-53. 2006
    ..This review will discuss the role of TAFI in the regulation of fibrinolysis and detail its regulation of activation and its potential therapeutic applications in thrombotic disease and bleeding disorders...
  15. pmc Plasma carboxypeptidase B downregulates inflammatory responses in autoimmune arthritis
    Jason J Song
    Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, California, USA
    J Clin Invest 121:3517-27. 2011
    ..Plasma carboxypeptidase B (CPB), which is activated by the thrombin/thrombomodulin complex, plays a procoagulant role during fibrin ..
  16. pmc Thrombin-activatable carboxypeptidase B cleavage of osteopontin regulates neutrophil survival and synoviocyte binding in rheumatoid arthritis
    Shadi A Sharif
    Stanford University School of Medicine, Stanford, California, CA 94305, USA
    Arthritis Rheum 60:2902-12. 2009
    ..Thrombin-activatable carboxypeptidase B (CPB), also called thrombin-activatable fibrinolysis inhibitor, removes the C-terminal arginine from OPN-R, ..
  17. ncbi TAFI, or plasma procarboxypeptidase B, couples the coagulation and fibrinolytic cascades through the thrombin-thrombomodulin complex
    L Bajzar
    Department of Biochemistry, Queen s University, Kingston, Ontario K7L 3N6, Canada
    J Biol Chem 271:16603-8. 1996
    ..is a recently discovered plasma protein that can be activated by thrombin-catalyzed proteolysis to a carboxypeptidase B-like enzyme that inhibits fibrinolysis...
  18. pmc In vivo regulation of plasminogen function by plasma carboxypeptidase B
    Carmen M Swaisgood
    Department of Molecular Cardiology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Clin Invest 110:1275-82. 2002
    ..The ability of plasma carboxypeptidase B (pCPB) to remove these residues suggests that it may act as a suppressor of these Plg functions...
  19. ncbi A comparative study of the activation peptide of carboxypeptidase B and trypsinogen as early predictors of the severity of acute pancreatitis
    J Saez
    Section of Gastroenterology and Department of Internal Medicine, Hospital General Universitario de Alicante, Alicante, Spain
    Pancreas 29:e9-14. 2004
    Serum and urine concentrations of the activation peptide of carboxypeptidase B (CAPAP) and urinary trypsinogen activation peptide (TAP) as prognostic markers in acute pancreatitis were compared.
  20. ncbi Amino acid sequence of Escherichia coli citrate synthase
    V Bhayana
    Biochemistry 23:2900-5. 1984
    ..coli enzyme is hexameric and allosterically inhibited by NADH, while the pig heart enzyme is dimeric and insensitive to that nucleotide...
  21. ncbi The amino acid sequence of the gamma-subunit of mouse submaxillary gland 7 S nerve growth factor
    K A Thomas
    J Biol Chem 256:9156-66. 1981
    ..An N-linked carbohydrate side chain is attached to Asn-78 in at least a majority of tee gamma-molecules...
  22. ncbi Human beta-casein. Amino acid sequence and identification of phosphorylation sites
    R Greenberg
    J Biol Chem 259:5132-8. 1984
    ..Locations of prolyl and charged residues are generally conserved for the three homologues. The sequence data indicate the existence of genetic polymorphism involving uncharged residues in human beta-casein...
  23. ncbi Amino acid sequence analysis of the asparagine-288 region of the carbohydrate variants of human plasminogen
    J R Powell
    Biochemistry 22:923-7. 1983
    ..Thus, the basis for the lack of the complex-type oligosaccharide in human plasminogen variant 2 does not reside in substitution of essential amino acid residues in the region of the Asn288-linked glycosylation site...
  24. ncbi The amino acid sequence of the D-galactose-binding protein from Escherichia coli B/r
    W C Mahoney
    J Biol Chem 256:4350-6. 1981
    ..The galactose-binding protein is the chemoreceptor initiating chemotaxis toward galactose, and it thus becomes the first protein component required for chemotaxis for which the primary structure is known. GM 24602..
  25. ncbi Amino acid sequence of rat alpha-lactalbumin: a unique alpha-lactalbumin
    R V Prasad
    Biochemistry 21:1479-82. 1982
    ..The amino acid sequence from residues 1 to 123 is similar to that of other alpha-lactalbumins. One possible explanation for the 17 amino acid extension is a mutation at the termination codon...
  26. ncbi On the specificity of carboxypeptidase N, a comparative study
    D Hendriks
    Department of Pharmaceutical Sciences, University of Antwerp, Belgium
    Biol Chem Hoppe Seyler 374:843-9. 1993
    ..Small synthetic peptide substrates were synthesized to confirm this hypothesis. This study shows that even with very low homology model building by homology can be employed to build models of sufficient quality to aid in drug design...
  27. ncbi TAFI and pancreatic carboxypeptidase B modulate in vitro capillary tube formation by human microvascular endothelial cells
    Ana H C Guimarães
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands
    Arterioscler Thromb Vasc Biol 27:2157-62. 2007
    ..Here we examined the involvement of thrombin activatable fibrinolysis inhibitor (TAFI) and pancreatic carboxypeptidase B (CPB) in an in vitro capillary tube formation system, which is largely plasminogen-dependent.
  28. ncbi Identification and characterization of two thrombin-activatable fibrinolysis inhibitor isoforms
    L Zhao
    Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804, USA
    Thromb Haemost 80:949-55. 1998
    ..When it is activated by the thrombin/thrombomodulin complex, activated TAFI exhibits carboxypeptidase B-like activity...
  29. ncbi A novel carboxypeptidase B that processes native beta-amyloid precursor protein is present in human hippocampus
    A Matsumoto
    Department of Radiation Biophysics and Genetics, and 2Department of Pathology, Kobe University School of Medicine, 7 Kusunoki cho, Chuo Ku, Kobe, 650 0017, Japan
    Eur J Neurosci 12:227-38. 2000
    ..vitro was purified and characterized; molecular analysis identified it as a novel protease belonging to the carboxypeptidase B (CPB) family...
  30. ncbi Metallocarboxypeptidases: emerging drug targets in biomedicine
    Joan L Arolas
    Institut de Biotecnologia i Biomedicina and Dept de Bioquímica i Biologia Molecular, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    Curr Pharm Des 13:349-66. 2007
    ..Future perspectives for the regulation of these enzymes through the use of more selective and potent inhibitors are also discussed in this review and combined with earlier observations in the field...
  31. ncbi The kinetics of plasminogen activation by thrombin-cleaved pro-urokinase and promotion of its activity by fibrin fragment E-2 and by tissue plasminogen activator
    J N Liu
    Vascular Research Laboratory, New England Deaconess Hospital, Harvard Medical School, Boston, MA 02215
    Blood 81:980-7. 1993
    ..However, this difference with pro-UK was nullified by carboxypeptidase B (CpB) treatment of thromb-UK to remove the C-terminal arginine on the A-chain...
  32. ncbi Possible association of chaperonin 60 with secretory proteins in pancreatic acinar cells
    I M Le Gall
    Department of Anatomy, Faculty of Medicine, University of Montreal, Quebec, Canada
    J Histochem Cytochem 44:743-9. 1996
    ..The highest association was found for amylase, lipase, and chymotrypsinogen, whereas trypsinogen and carboxypeptidase B showed much lower association values...
  33. ncbi Isoforms of procarboxypeptidase B, (pancreas-specific protein, PASP) in human serum, pancreatic tissue and juice
    R Fernstad
    Department of Surgery, Sabbatsberg Hospital, Stockholm, Sweden
    Scand J Clin Lab Invest Suppl 213:9-17. 1993
    ..Bands corresponding to PASP/PCPB as well as carboxypeptidase B (CPB) were seen simultaneously. During recovery PASP in serum was normalised and the CPB band disappeared...
  34. ncbi Mapping the pro-region of carboxypeptidase B by protein engineering. Cloning, overexpression, and mutagenesis of the porcine proenzyme
    S Ventura
    Departament de Bioquimica i Biologia Molecular, Unitat de Ciències, and Institut de Biologia Fonamental, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    J Biol Chem 274:19925-33. 1999
    ..connecting region after the first tryptic cut allows subsequent proteolytic processing and the expression of carboxypeptidase B activity...
  35. ncbi Insulin-like growth factor-binding protein-5 activates plasminogen by interaction with tissue plasminogen activator, independently of its ability to bind to plasminogen activator inhibitor-1, insulin-like growth factor-I, or heparin
    Alice M Sorrell
    Hannah Research Institute, Ayr KA6 5HL, United Kingdom
    J Biol Chem 281:10883-9. 2006
    ..Thus IGFBP-5 can induce cell death by both sequestering IGF-I and enhancing plasmin generation...
  36. ncbi Characterization and cDNA cloning of midgut carboxypeptidases from Trichoplusia ni
    Ping Wang
    Department of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva 14456, USA
    Insect Biochem Mol Biol 34:831-43. 2004
    Carboxypeptidase A and carboxypeptidase B activities from the midgut of Trichoplusia ni larvae were characterized. In the T...
  37. pmc Distribution of manganese in rat pancreas and identification of its primary binding protein as pro-carboxypeptidase B
    H Kodama
    National Institute for Environmental Studies, Ibaraki, Japan
    Biochem J 278:857-62. 1991
    ..On the basis of enzymic and chemical characterization of the protein, it was identified as a zymogen of carboxypeptidase B (pro-carboxypeptidase B, pro-CPB). The elution profiles of the protein by h.p.l.c.-i.c.p...
  38. ncbi Inhibition of cell surface mediated plasminogen activation by a monoclonal antibody against alpha-Enolase
    Roser Lopez-Alemany
    Centre d Oncologia Molecular C O M, Institut de Recerca Oncolgica IRO, Barcelona, Spain
    Am J Hematol 72:234-42. 2003
    ..These data suggest that, although leukocytic cells display several molecules that bind plasminogen, alpha-enolase is responsible for the majority of the promotion of plasminogen activation on the surfaces of leukocytic cells...
  39. ncbi Pancreastatin--a novel regulatory peptide?
    W E Schmidt
    Department of Medicine, Georg August University of Gottingen, Germany
    Acta Oncol 30:441-9. 1991
    ..Whether or not pancreastatin can be classified as a novel peptide hormone that under physiological conditions plays a role in the regulation of the endocrine and exocrine pancreas, is still a matter of controversy...
  40. ncbi The intracellular proteolytic processing of extracellular superoxide dismutase (EC-SOD) is a two-step event
    Dorte Aa Olsen
    Department of Molecular Biology, University of Aarhus, DK 8000 Arhus, Denmark
    J Biol Chem 279:22152-7. 2004
    ..The mutation does not prevent proteolysis of the ECM-binding region but prevents a carboxypeptidase B-like enzyme from trimming residues beyond Gly(213)...
  41. ncbi Amyloid endostatin induces endothelial cell detachment by stimulation of the plasminogen activation system
    Arie Reijerkerk
    Department of Medical Oncology, University Medical Center Utrecht, Utrecht, The Netherlands
    Mol Cancer Res 1:561-8. 2003
    ..of plasminogen activation, vitronectin degradation, and endothelial cell detachment is inhibited by carboxypeptidase B, indicating an essential role for carboxyl-terminal lysines...
  42. ncbi Generation and characterization of a highly stable form of activated thrombin-activable fibrinolysis inhibitor
    Pauline F Marx
    Department of Vascular Medicine, Academic Medical Center, University of Amsterdam, 1100 DD Amsterdam, The Netherlands
    J Biol Chem 279:6620-8. 2004
    Activated thrombin-activable fibrinolysis inhibitor (TAFIa) is a carboxypeptidase B that can down-regulate fibrinolysis. TAFIa is a labile enzyme that can be inactivated by conformational instability or proteolysis...
  43. ncbi Impaired healing of cutaneous wounds and colonic anastomoses in mice lacking thrombin-activatable fibrinolysis inhibitor
    E A te Velde
    Department of Medical Oncology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Thromb Haemost 1:2087-96. 2003
    ..Although our study shows that tissue repair, including re-epithelialization and scar formation, occurs in TAFI-deficient mice, TAFI appears to be important for appropriate organization of the healing process...
  44. ncbi Comparison of the NMR solution structure with the X-ray crystal structure of the activation domain from procarboxypeptidase B
    M Billeter
    Institut fur Molekularbiologie und Biophysik, Zurich, Switzerland
    J Biomol NMR 2:1-10. 1992
    ..These regions of increased structural disorder in the free domain coincide only partially with the interface to the enzyme domain in the proenzyme...
  45. pmc Intracellular proteolysis of pancreatic zymogens
    F S Gorelick
    Department of Medicine, Department of Veterans Affairs Medical Center, West Haven, Connecticut 06516
    Yale J Biol Med 65:407-20; discussion 437-40. 1992
    ..These findings indicate that pathways for zymogen activation as well as degradation of active enzyme forms are present within the pancreatic acinar cell...
  46. ncbi Carboxypeptidase B inhibitors reduce tissue factor-induced renal microthrombi in rats
    Yuko Muto
    Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd, 760 Morooka cho, Kohoku Ku, Yokohama 222 8567, Japan
    Eur J Pharmacol 461:181-9. 2003
    ..residues from partially degraded fibrin are important for the binding and activation of plasminogen, and carboxypeptidase B, an active form of procarboxypeptidase B, has been shown to inhibit fibrinolysis by eliminating these ..
  47. pmc Three-dimensional structure of porcine procarboxypeptidase B: a structural basis of its inactivity
    M Coll
    Max Planck Institut fur Biochemie, Martinsried bei Munchen, FRG
    EMBO J 10:1-9. 1991
    Procarboxypeptidase B is converted to enzymatically active carboxypeptidase B by limited proteolysis catalysed by trypsin, removing the long N-terminal activation segment of 95 amino acids...
  48. pmc The NMR structure of the activation domain isolated from porcine procarboxypeptidase B
    J Vendrell
    Institut fur Molekularbiologie und Biophysik, Eidgenössische, Technische Hochschule Hönggerberg, Zurich, Switzerland
    EMBO J 10:11-5. 1991
    ....
  49. pmc Human mast cell carboxypeptidase. Purification and characterization
    S M Goldstein
    Department of Dermatology, University of California, San Francisco 94143
    J Clin Invest 83:1630-6. 1989
    ..amino acid sequence of mast cell carboxypeptidase demonstrated 65% positional identity with human pancreatic carboxypeptidase B, but only 19% with human carboxypeptidase A...
  50. ncbi Purification and properties of five different forms of human procarboxypeptidases
    R Pascual
    Departament de Bioquimica i Biologia Molecular, Facultat de Ciencies, Universitat Autonoma de Barcelona, Spain
    Eur J Biochem 179:609-16. 1989
    ..N-terminal extended sequence analysis carried out on these proenzymes confirm that they constitute different isologous forms...
  51. ncbi Detailed molecular comparison between the inhibition mode of A/B-type carboxypeptidases in the zymogen state and by the endogenous inhibitor latexin
    R García-Castellanos
    Institut de Biologia Molecular de Barcelona, C I D C S I C, C Jordi Girona 18 26, 08034, Barcelona, Spain
    Cell Mol Life Sci 62:1996-2014. 2005
    ..The main contacting structure of latexin is similar to the one employed for PD inhibition. In both cases, active-site blocking relies mainly on a loop provided by the central part of a beta sheet...
  52. ncbi The intrinsic threshold of the fibrinolytic system is modulated by basic carboxypeptidases, but the magnitude of the antifibrinolytic effect of activated thrombin-activable fibrinolysis inhibitor is masked by its instability
    John B Walker
    Henderson Research Centre and McMaster University, 711 Concession Street, Hamilton, Ontario L8V 1C3, Canada
    J Biol Chem 279:27896-904. 2004
    ..on fibrinolysis under conditions of constant carboxypeptidase activity, we employed pancreatic carboxypeptidase B (CPB), a homologous, stable basic carboxypeptidase, as a surrogate for TAFIa...
  53. ncbi Beta-amyloid (Abeta) causes detachment of N1E-115 neuroblastoma cells by acting as a scaffold for cell-associated plasminogen activation
    Onno Kranenburg
    Department of Medical Oncology, University Medical Center Utrecht, Utrecht, The Netherlands
    Mol Cell Neurosci 28:496-508. 2005
    ..Cell detachment is inhibited by carboxypeptidase B (CPB), an enzyme that blocks plasmin formation by cleaving off C-terminal lysine residues...
  54. ncbi Alpha-2-antiplasmin: a serpin with two separate but overlapping reactive sites
    J Potempa
    Institute of Molecular Biology, Jagiellonian University, Cracow, Poland
    Science 241:699-700. 1988
    ..Thus, a naturally occurring plasma serine proteinase inhibitor can have multiple specificities through interactions at adjacent sites. It also illustrates the potential flexibility of the reactive site loop in this class of inhibitors...
  55. ncbi Further studies on the human pancreatic binary complexes involving procarboxypeptidase A
    M Moulard
    Centre de Biochimie et de Biologie Moleculaire du CNRS, Marseille, France
    FEBS Lett 261:179-83. 1990
    ..Only one type of procarboxypeptidase B, the B1 type, has been detected in the analyzed pancreatic juices, thus emphasizing the previously observed genetic differences between individuals...
  56. ncbi Analysis of the activation process of porcine procarboxypeptidase B and determination of the sequence of its activation segment
    F J Burgos
    Departament de Bioquimica, Universitat Autonoma de Barcelona, Spain
    Biochemistry 30:4082-9. 1991
    The molecular events which lead to the proteolytic transformation of porcine procarboxypeptidase B (PCPB) in carboxypeptidase B (CPB) have been determined...
  57. ncbi Topological analysis of NhaA, a Na+/H+ antiporter from Escherichia coli
    A Rothman
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 271:32288-92. 1996
    ..to test the location of the C terminus, right-side-out and inside-out membrane vesicles were digested with carboxypeptidase B and probed with an antibody raised against a synthetic peptide whose sequence was based on the C terminus ..
  58. ncbi Factor XI dependent and independent activation of thrombin activatable fibrinolysis inhibitor (TAFI) in plasma associated with clot formation
    B N Bouma
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA
    Thromb Haemost 82:1703-8. 1999
    ..Our results indicate that activation of TAFI in serum after clot formation can be quantitated and that it takes place in both factor XI-dependent and factor XI-independent mechanisms...
  59. ncbi Genetic basis of phenotypic plasticity for predator-induced morphological defenses in anuran tadpole, Rana pirica, using cDNA subtraction and microarray analysis
    Tsukasa Mori
    Laboratory of Marine Molecular Biochemistry, Department of Nihon University College of Bioresource Sciences, Kameino 1866, Fujisawa 252 8510, Japan
    Biochem Biophys Res Commun 330:1138-45. 2005
    ..In the subtraction, carboxypeptidase B, trypsinogen, elastase I, fibrinogen, elastase II, triacyl-glycerol lipase, and alpha1-antitrypsin genes ..
  60. ncbi Does an enzyme other than thrombin contribute to unexpected changes in the levels of the different forms of thrombin activatable fibrinolysis inhibitor in patients with hemophilia A, hemophilia B and von Willebrand disease?
    J P Antovic
    Coagulation Research, Department of Surgical Sciences, Karolinska University Hospital, Stockholm, Sweden
    Scand J Clin Lab Invest 64:745-51. 2004
    ..Pro-TAFI is activated by thrombin/thrombomodulin complex or by plasmin to a carboxypeptidase B-like enzyme (TAFI) of 35.8 kD molecular weight...
  61. ncbi Human carboxypeptidase M. Purification and characterization of a membrane-bound carboxypeptidase that cleaves peptide hormones
    R A Skidgel
    Department of Anesthesiology, College of Medicine, University of Illinois, Chicago 60680
    J Biol Chem 264:2236-41. 1989
    A membrane-bound neutral carboxypeptidase B-like enzyme was solubilized from human placental microvilli with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) and purified to homogeneity by ion-exchange chromatography and ..
  62. ncbi Mediators of C5a-induced bronchoconstriction in the guinea pig
    J F Regal
    Department of Pharmacology, University of Minnesota Duluth, School of Medicine
    Int Arch Allergy Appl Immunol 84:414-23. 1987
    ..Conversion of the C5a in the mixture to C5ades arg by carboxypeptidase B digestion did not significantly alter the magnitude of the bronchoconstriction...
  63. ncbi Characterization of the gene encoding human TAFI (thrombin-activable fibrinolysis inhibitor; plasma procarboxypeptidase B)
    M B Boffa
    Department of Biochemistry, Queen s University, Kingston, Ontario, Canada
    Biochemistry 38:6547-58. 1999
    ..fibrinolysis inhibitor (TAFI) is a recently described human plasma zymogen that is related to pancreatic carboxypeptidase B. The active form of TAFI (TAFIa), which is formed by thrombin cleavage of the zymogen, likely inhibits ..
  64. ncbi Activated thrombin-activatable fibrinolysis inhibitor reduces the ability of high molecular weight fibrin degradation products to protect plasmin from antiplasmin
    Mark Schneider
    Departments of Biochemistry and Medicine, Queen s University, Kingston Ontario K7L 3N6, Canada
    J Biol Chem 279:13340-5. 2004
    Activated thrombin-activable fibrinolysis inhibitor (TAFIa) is a carboxypeptidase B-like plasma enzyme that can slow clot lysis by removing lysine residues exposed on fibrin as it is cleaved by plasmin...
  65. ncbi Post-translational modifications of human thrombin-activatable fibrinolysis inhibitor (TAFI): evidence for a large shift in the isoelectric point and reduced solubility upon activation
    Zuzana Valnickova
    Center for Insoluble Protein Structure inSPIN at the Department of Molecular Biology, Science Park, University of Aarhus, Gustav Wied s Vej 10C, 8000 Aarhus C, Denmark
    Biochemistry 45:1525-35. 2006
    ..However, after activation, TAFIa loses 80% of the attached glycans, generating a large shift in pI and a propensity to precipitate. These changes are likely to significantly affect the properties of TAFIa as compared to TAFI...
  66. ncbi Characterization of two types of mitochondrial creatine kinase isolated from normal human cardiac muscle and brain tissue
    F Kanemitsu
    Clinical Laboratories, Kurashiki Central Hospital, Japan
    Electrophoresis 21:266-70. 2000
    ..55, 6.75, 6.85, and 6.95. New bands appearing at pI 6.65 and 6.75 after treatment of sMi-CK with carboxypeptidase B were found to be delysined forms...
  67. ncbi Spontaneous exocrine pancreas hypoplasia in specific pathogen-free C3HeB/FeJ and 101/H mouse pups causes steatorrhea and runting
    Michael T Cheeseman
    Pathology Group, Mary Lyon Centre, Medical Research Council Harwell, Didcot, Oxfordshire, UK
    Comp Med 57:210-6. 2007
    ..comprising cytokeratin-negative duct-like structures but lacking recognizable acinar cells with their normal carboxypeptidase B-positive zymogen granules...
  68. ncbi Pro-carboxypeptidase R is an acute phase protein in the mouse, whereas carboxypeptidase N is not
    T Sato
    Department of Molecular Biology, Nagoya City University Medical School, Nagoya, Japan
    J Immunol 165:1053-8. 2000
    ..Human proCPR, also known as thrombin-activatable fibrinolysis inhibitor, plasma pro-carboxypeptidase B, and pro-carboxypeptidase U, is a plasma zymogen activated during coagulation...
  69. ncbi Bioavailability of co-supplemented organic and inorganic zinc and selenium sources in a white fishmeal-based rainbow trout (Oncorhynchus mykiss) diet
    S A Rider
    School of Biological Sciences, The University of Plymouth, Plymouth, Devon, UK
    J Anim Physiol Anim Nutr (Berl) 94:99-110. 2010
    ..05) and neither forms of Se affected glutathione peroxidise activity (p = 0.059). Alkaline phosphatase and carboxypeptidase B were not affected by Zn supplementation (p = 0.51 and p = 0.88 respectively)...
  70. pmc Pollution-affected fish hepatic transcriptome and its expression patterns on exposure to cadmium
    M Auslander
    Israel Oceanographic and Limnological Research, Haifa 31080, Israel
    Mar Biotechnol (NY) 10:250-61. 2008
    ..Prominently differentially expressed genes included elastase 4, carboxypeptidase B, trypsinogen, perforin, complement C31, cytochrome P450 2K5, ceruloplasmin, carboxyl ester lipase, and ..
  71. ncbi Total thrombin-activatable fibrinolysis inhibitor (TAFI) antigen and pro-TAFI in patients with haemophilia A
    J Antovic
    Coagulation Research, Department of Surgical Sciences, Karolinska Institutet, Karolinska Hospital, Stockholm, Sweden
    Haemophilia 7:557-60. 2001
    ..In this paper we use new nomenclature for TAFI, and we believe that this recommended terminology for different forms of TAFI can simplify further standardization in TAFI investigation...
  72. pmc Thrombomodulin mutations in atypical hemolytic-uremic syndrome
    Mieke Delvaeye
    VIB K U Leuven Vesalius Research Center, Leuven, Belgium
    N Engl J Med 361:345-57. 2009
    ..Genetic factors in the remaining cases are unknown. We studied the role of thrombomodulin, an endothelial glycoprotein with anticoagulant, antiinflammatory, and cytoprotective properties, in atypical hemolytic-uremic syndrome...
  73. ncbi Enhancement of fibrinolysis by EF6265 [(S)-7-amino-2-[[[(R)-2-methyl-1-(3-phenylpropanoylamino)propyl]hydroxyphosphinoyl] methyl]heptanoic acid], a specific inhibitor of plasma carboxypeptidase B
    Kokichi Suzuki
    Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd, Yokohama, Japan
    J Pharmacol Exp Ther 309:607-15. 2004
    ..as thrombin-activatable fibrinolysis inhibitor (TAFI), is converted by thrombin into the active enzyme, carboxypeptidase B (CPB)/activated TAFI...
  74. ncbi [Thrombin activatable fibrinolysis inhibitor (TAFI) in allergic asthma patients]
    Izabela Kemona-Chetnik
    Klinika Alergologii i Chorob Wewnetrznych, Akademii Medycznej w Białymstoku
    Przegl Lek 63:1281-5. 2006
    ..Thrombin activatable fibrinolysis inhibitor (TAFI) is a carboxypeptidase B-like proenzyme which after activation by a thrombin-thrombomodulin complex inhibits fibrinolysis...
  75. ncbi Myocardial infarction and the balance between fibrin deposition and removal
    M Nesheim
    Department of Biochemistry, Queen s University, Kingston, Ontario, Canada
    Ital Heart J 2:641-5. 2001
    ..substance, activated TAFI (thrombin activatable fibrinolysis inhibitor, also known as plasma carboxypeptidase B or carboxypeptidase U)...
  76. ncbi Thrombomodulin suppresses invasiveness of HT1080 tumor cells by reducing plasminogen activation on the cell surface through activation of thrombin-activatable fibrinolysis inhibitor
    Toshiyuki Higuchi
    Department of Molecular and Cellular Pathophysiology, Showa Pharmaceutical University, Tokyo, Japan
    Biol Pharm Bull 32:179-85. 2009
    ..The active form of TAFI (TAFIa) contributes to inhibition of plasmin formation through its carboxypeptidase B (CPB)-like activity...
  77. ncbi Role of isoleucine residues 182 and 183 in thrombin-activatable fibrinolysis inhibitor
    P F Marx
    Department of Vascular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    J Thromb Haemost 3:1293-300. 2005
    ..and characterized a TAFI mutant in which Ile182 and Ile183 were changed into the residues found in pancreas carboxypeptidase B at corresponding positions, Arg and Glu...
  78. ncbi Changes in neutrophil met-enkephalin containing peptides in episodic cluster headache
    M L Figuerola
    , CONICET, , Buenos Aires, Argentina
    Headache 31:406-8. 1991
    ..Neutrophil met-enkephalin containing peptide levels (after sequential enzymatic digestion with trypsin and carboxypeptidase B) were determined by radioimmunoassay with specific antiserum...
  79. ncbi Carboxypeptidase E activity is deficient in mice with the fat mutation. Effect on peptide processing
    L D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 271:30619-24. 1996
    ..Treatment of the Cpefat/Cpefat brain extract with carboxypeptidase B restores the level of Leu-enkephalin to the level in control brain...
  80. ncbi Regulation of fibrinolysis in plasma by TAFI and protein C is dependent on the concentration of thrombomodulin
    L O Mosnier
    Dept of Haematology, University Medical Center, Utrecht, The Netherlands
    Thromb Haemost 85:5-11. 2001
    Thrombin activatable fibrinolysis inhibitor (TAFI) is a carboxypeptidase B-like proenzyme, that after activation down regulates fibrinolysis. TAFI is activated by thrombin in the presence of the cofactor thrombomodulin (TM)...
  81. ncbi Risk of ischemic stroke associated with functional thrombin-activatable fibrinolysis inhibitor plasma levels
    A Santamaria
    Department of Hematology, Hospital de la Santa Creu i Sant Pau, C Sant Antoni Ma Claret, 167, 08025 Barcelona, Spain
    Stroke 34:2387-91. 2003
    ..The relevance of TAFI in thromboembolic disease is unclear. We have investigated the risk of ischemic stroke (IS) in relation to plasma levels of functional TAFI...
  82. ncbi Thrombin-activatable fibrinolysis inhibitor (TAFI, plasma procarboxypeptidase B, procarboxypeptidase R, procarboxypeptidase U)
    B N Bouma
    Thrombosis and Haemostasis Laboratory, Department of Haematology, University Medical Center, Utrecht, The Netherlands
    J Thromb Haemost 1:1566-74. 2003
    ..However, it cannot be ruled out that TAFI has other, as yet undefined, functions in biology...
  83. ncbi Discriminating between cell surface and intracellular plasminogen-binding proteins: heterogeneity in profibrinolytic plasminogen-binding proteins on monocytoid cells
    S B Hawley
    Scripps Research Institute, Department of Vascular Biology, La Jolla, CA 92037, USA
    Thromb Haemost 84:882-90. 2000
    ..activation expose a carboxyl-terminal lysine on the cell surface and are sensitive to proteolysis by carboxypeptidase B (CpB)...
  84. ncbi Enhancement of endogenous plasminogen activator-induced thrombolysis by argatroban and APC and its control by TAFI, measured in an arterial thrombolysis model in vivo using rat mesenteric arterioles
    Masaru Hashimoto
    Laboratory of Physiology, Faculty of Nutrition, Kobe Gakuin University and Kobe Gakuin University High Technology Research Centre, Japan
    Thromb Haemost 87:110-3. 2002
    ..was to examine the role of TAFI in these fibrinolytic mechanisms in vivo using purified porcine pancreatic carboxypeptidase B (PPCPB) and a specific TAFIa inhibitor, potato tuber carboxypeptidase B inhibitor (PTCI) in a newly ..
  85. ncbi Human skin mast cell carboxypeptidase: functional characterization, cDNA cloning, and genealogy
    M Natsuaki
    Department of Dermatology, University of California, San Francisco 94143 0536
    J Invest Dermatol 99:138-45. 1992
    ..trees constructed from the known carboxypeptidase sequences suggested that MC-CPA most likely evolved from a carboxypeptidase B-like enzyme, independent of the pancreatic CPA...
  86. ncbi Enzyme leakage, trypsinogen activation, and inflammatory response in endoscopic retrograde cholangiopancreatography-induced pancreatitis
    Ulf Petersson
    Department of Surgery, Surgical Pathophysiology, and Radiology, Malmo University Hospital, University of Lund, Malmo, Sweden
    Pancreas 24:321-8. 2002
    ..Endoscopic retrograde cholangiopancreatography (ERCP)-induced pancreatitis (EIP) provides an opportunity to study different pathophysiologic events early in the course of acute pancreatitis...
  87. ncbi Characterization of thrombin activatable fibrinolysis inhibitor in normal and acquired haemostatic dysfunction
    Cheng Hock Toh
    Roald Dahl Haemostasis and Thrombosis Centre, Royal Liverpool University Hospital, Liverpool, UK
    Blood Coagul Fibrinolysis 14:S69-71. 2003
    Thrombin activatable fibrinolysis inhibitor (TAFI) is a carboxypeptidase B-like proenzyme, which is synthesized in the liver and circulates in the blood at a concentration of about 275 nmol/l...
  88. ncbi Analysis of the pancreatic low molecular weight proteome in an animal model of acute pancreatitis
    Olivier Lassout
    Clinical Proteomics Laboratory, Department of Genetics and Laboratory Medicine, Geneva University Hospitals, Biomedical Proteomics Research Group, Department of Bioinformatics and Structural Biology, Geneva Faculty of Medicine, Geneva, Switzerland
    J Proteome Res 9:4535-44. 2010
    ..Moreover, for murinoglobulin-1 or carboxypeptidase B, peptide pattern could be related to protein function...
  89. ncbi Role of zinc ions in activation and inactivation of thrombin-activatable fibrinolysis inhibitor
    Pauline F Marx
    Thrombosis and Haemostasis Laboratory, Department of Haematology, University Medical Centre, P O Box 85500, 3508 GA Utrecht, The Netherlands
    Biochemistry 41:1211-6. 2002
    Thrombin-activatable fibrinolysis inhibitor (TAFI) circulates as an inactive proenzyme of a carboxypeptidase B-like enzyme (TAFIa)...
  90. ncbi Degradation pathway of kinins in tumor ascites and inhibition by kininase inhibitors: analysis by HPLC
    Y Matsumura
    Department of Microbiology, Kumamoto University Medical School, Japan
    Agents Actions 29:172-80. 1990
    ..The carboxypeptidase A and carboxypeptidase B inhibitor, benzyl malic acid, failed to block degradation of both kinins...
  91. ncbi Thrombin activatable fibrinolysis inhibitor: not just an inhibitor of fibrinolysis
    Laszlo Bajzar
    McMaster University, Hamilton, Ontario, Canada
    Crit Care Med 32:S320-4. 2004
    ..To review the activation of thrombin activatable fibrinolysis inhibitor (TAFI) and activity of activated TAFI (TAFIa) as it relates to the regulation of both fibrinolytic and proinflammatory substances...
  92. ncbi Secretory expression of active clostripain in Escherichia coli
    Chang Kyu Kim
    College of Animal Bioscience and Technology, Konkuk University, Seoul, Republic of Korea
    J Biotechnol 131:346-52. 2007
    ..The recombinant clostripain and porcine carboxypeptidase B were used in the conversion of a proinsulin fusion protein into insulin...
  93. ncbi Interaction of Leishmania mexicana promastigotes with the plasminogen-plasmin system
    L Avilan
    Laboratorio de Immunología de Parasitosis LABINPAR, Departamento de Biologia, Facultad de Ciencias, Universidad de Los Andes, Merida, Venezuela
    Mol Biochem Parasitol 110:183-93. 2000
    ..C-terminal lysine residues are involved in plasminogen binding to cells, since carboxypeptidase B treatment reduced this binding by 34%...
  94. ncbi cDNA cloning and phylogenetic analysis of pancreatic serine proteases from Japanese flounder, Paralichthys olivaceus
    Tohru Suzuki
    Metabolism Section, National Research Institute of Aquaculture, Fisheries Research Agency, Nansei, 516 0193, Mie, Japan
    Comp Biochem Physiol B Biochem Mol Biol 131:63-70. 2002
    ..cDNAs of two species of carboxypeptidase A, one carboxypeptidase B and lipase were also obtained...
  95. ncbi Thrombin-activatable fibrinolysis inhibitor (TAFI, plasma procarboxypeptidase B, procarboxypeptidase R, procarboxypeptidase U)
    B N Bouma
    Thrombosis and Hemostasis Laboratory, Department of Haematology, University Medical Center, Utrecht, The Netherlands
    Thromb Res 101:329-54. 2001
    ..In this review, we will describe the properties of basic carboxypeptidases with the emphasis on the role of TAFI in coagulation and fibrinolysis. It cannot be ruled out, however, that TAFI has other, yet undefined, functions in biology...
  96. ncbi Plasminogen and tissue plasminogen activator interact with antithrombin III
    A K Dudani
    Bureau of Biologics and Radiopharmaceuticals, Therapeutic Products Programme, Health Canada, and Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Canada
    Thromb Res 99:635-41. 2000
    ..In converse experiments, antithrombin III also interacted with immobilized plasminogen. Using carboxypeptidase B digestion, the plasminogen-binding site of antithrombin III was localized to the carboxy-terminus lysine of ..
  97. ncbi Amino acid sequence of the N-terminus and selected tryptic peptides of the active subunit of human plasma carboxypeptidase N: comparison with other carboxypeptidases
    R A Skidgel
    Department of Anesthesiology, University of Illinois, Chicago College of Medicine 60612
    Biochem Biophys Res Commun 154:1323-9. 1988
    ..of bovine carboxypeptidase H (43% identity) and to a lesser extent with carboxypeptidase A (29% identity) or carboxypeptidase B (18% identity)...
  98. pmc Cloning and characterization of the novel gene for mast cell carboxypeptidase A
    D S Reynolds
    Department of Medicine, Harvard Medical School, Boston, Massachusetts
    J Clin Invest 89:273-82. 1992
    ..of the MC-CPA gene and those of three rat pancreatic carboxypeptidase genes (carboxypeptidase A1 and A2, and carboxypeptidase B [CPB])...
  99. ncbi Processing of proenkephalin in adrenal chromaffin cells
    S P Wilson
    Department of Pharmacology, University of South Carolina School of Medicine, Columbia 29208
    J Neurochem 57:876-81. 1991
    ..Fractions containing proenkephalin fragments were digested with trypsin and carboxypeptidase B to liberate Met-enkephalin sequences and subjected to a second HPLC step to demonstrate association of ..
  100. ncbi An experimental test of dietary enzyme modulation in pine warblers Dendroica pinus
    D J Levey
    Department of Zoology, P O Box 118525, University of Florida, Gainesville, FL 32611 8525
    Physiol Biochem Zool 72:576-87. 1999
    ..measured activity of amylase, maltase, sucrase, aminopeptidase-N, trypsin, chymotrypsin, carboxypeptidase A, carboxypeptidase B, pancreatic lipase, and carboxyl ester lipase...
  101. ncbi Regulated secretory proteins in the exocrine pancreas aggregate under conditions that mimic the trans-Golgi network
    S D Freedman
    Laboratory of Cell and Molecular Biology, Charles A Dana Research Institute, Thorndike Laboratory, Boston, MA
    Biochem Biophys Res Commun 197:992-9. 1993
    ..In the absence of secretory granule membranes, granule contents display an "intrinsic" property for reversible, pH-dependent aggregation under conditions of mild acidification...

Research Grants11

  1. Cross-talk Between Coagulation, Inflammation, and Immunity
    Lawrence L Leung; Fiscal Year: 2013
    ..pCPB KO mice, we have obtained compelling data to support our general hypothesis that thrombin-activatable carboxypeptidase B (CPB or TAFIa) plays a broad anti-inflammatory role in vivo;the targets for CPB include bradykinin, C5a, the ..
  2. The Role of Carboxypeptidase B and its Substrates in Rheumatoid Arthritis
    William H Robinson; Fiscal Year: 2013
    ..b>Carboxypeptidase B (CPB) is well established to play an anti-fibrinolytic role by removing C-terminal lysine residues from ..
  3. ENZYMES INVOLVED IN DYNORPHIN BIOSYNTHESIS
    Lakshmi Devi; Fiscal Year: 1993
    ..are synthesized as a precursor protein, prodynorphin, that through limited proteolysis by trypsin-like and carboxypeptidase B-like enzymes gives rise to alpha- and beta-neoendorphin...
  4. STRUCTURE AND FUNCTION OF PANCREATIC ENZYMES
    C Hirs; Fiscal Year: 1980
    ..and their membranes in the acinar pancreas and by studies of the relation between structure, specificity and function in the enzymes carboxypeptidase A, carboxypeptidase B and elastase, as also of their respective zymogen precursors.
  5. CELL BIOLOGY OF CARBOXYPEPTIDASE D
    Lloyd Fricker; Fiscal Year: 2003
    ..This will provide a better understanding of the proteins, either known or novel, that interact with CPD. These studies on CPD will advance our knowledge of protein trafficking in neuroendocrine cells. ..
  6. Function of Carboxypeptidase D
    Lloyd Fricker; Fiscal Year: 2008
    ..The overall goal of this proposal is to understand the role of each domain within CPD, using a simple organism for which CPD mutants exist and which is amenable to transgenic analysis. ..
  7. Quantitative Peptidomics of Food Deprivation
    Lloyd Fricker; Fiscal Year: 2005
    ..The studies in this initial R21 will provide an unbiased survey of neuropeptides that are altered by food deprivation, and which may therefore be involved in regulating food intake and/or body weight. ..
  8. REGULATION OF NEUROPEPTIDE PROCESSING ENZYMES
    Lloyd Fricker; Fiscal Year: 2002
    ..The results of these studies will provide a better understanding of the enzymes involved with peptide biosynthesis. ..
  9. Direct Cellular Effects of Blood Coagulation Proteases
    LAURENT MOSNIER; Fiscal Year: 2007
    ..End of Abstract) [unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
  10. Peptidomics of Cocaine and Amphetamine Abuse
    Lloyd Fricker; Fiscal Year: 2005
    ..Taken together, these studies will provide a better understanding of the regulation of neuropeptides, both known and novel, in drug abuse. ..
  11. Pancreatic zymogen activation in a reconstituted system
    Edwin Thrower; Fiscal Year: 2006
    ..We plan to use this system to identify the compartment(s) responsible for zymogen processing and to examine the ATP and calcium-dependent mechanism of activation. [unreadable] [unreadable] [unreadable]..