Genomes and Genes
Summary: A family of SERINE ENDOPEPTIDASES isolated from Bacillus subtilis. EC 3.4.21.-
Publications227 found, 100 shown here
- Subcellular discharge of a serine protease mediates release of invasive malaria parasites from host erythrocytesSharon Yeoh
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
Cell 131:1072-83. 2007..Our findings reveal the presence in the malarial parasitophorous vacuole of a regulated, PfSUB1-mediated proteolytic processing event required for release of viable parasites from the host erythrocyte...
- Primary structure and expression of a pathogen-induced protease (PR-P69) in tomato plants: Similarity of functional domains to subtilisin-like endoproteasesP Tornero
Instituto de Biologia Molecular y Celular de Plantas, Universidad Politécnica Consejo Superior de Investigaciones Científicas, Valencia, Spain
Proc Natl Acad Sci U S A 93:6332-7. 1996....
- Production of the subtilase AB5 cytotoxin by Shiga toxin-negative Escherichia coliRosangela Tozzoli
Dipartimento di Sanita Pubblica Veterinaria e Sicurezza Alimentare, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
J Clin Microbiol 48:178-83. 2010..These results indicate that SubAB production is not restricted to STEC and suggest that a subAB-tia putative pathogenicity island is involved in the dissemination of subAB genes, as an alternative to plasmid pO113...
- A genomic cluster containing four differentially regulated subtilisin-like processing protease genes is in tomato plantsL Jorda
Instituto de Biología Molecular y Celular de Plantas IBMCP, Universidad Politécnica Consejo Superior de Investigaciones Científicas, Camino de Vera s n, 46022 Valencia, Spain
J Biol Chem 274:2360-5. 1999....
- A new pathogenicity island carrying an allelic variant of the Subtilase cytotoxin is common among Shiga toxin producing Escherichia coli of human and ovine originV Michelacci
European Reference Laboratory for Escherichia coli, Istituto Superiore di Sanita, Dipartimento di Sanita Pubblica Veterinaria e Sicurezza Alimentare, Rome, Italy
Clin Microbiol Infect 19:E149-56. 2013..Further studies are needed to assess the role of tia and/or other genes carried by SE-PAI in the colonization of the host intestinal mucosa...
- Incorporation of a non-human glycan mediates human susceptibility to a bacterial toxinEmma Byres
Protein Crystallography Unit and ARC Centre of Excellence for Structural and Functional Microbial Genomics, Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
Nature 456:648-52. 2008..Ironically, foods rich in Neu5Gc are the most common source of STEC contamination. Thus a bacterial toxin's receptor is generated by metabolic incorporation of an exogenous factor derived from food...
- Activation of a recombinant membrane type 1-matrix metalloproteinase (MT1-MMP) by furin and its interaction with tissue inhibitor of metalloproteinases (TIMP)-2H Sato
Department of Molecular Virology and Oncology, Kanazawa University, Japan
FEBS Lett 393:101-4. 1996..Tissue inhibitor of metalloproteinases (TIMP)-2 inhibited activation of progelatinase A by forming a stable complex with activated MT1-MMP...
- Inhibition of furin-mediated cleavage activation of HIV-1 glycoprotein gp160S Hallenberger
Institut fur Virologie, Philips Universität Marburg, Germany
Nature 360:358-61. 1992..We show here that the glycoprotein of HIV-1, which has the same protease recognition motif as the FPV haemagglutinin, is also activated by furin...
- Inferring hypotheses on functional relationships of genes: Analysis of the Arabidopsis thaliana subtilase gene familyCarsten Rautengarten
Institut für Biochemie und Biologie, Genetik, Universitat Potsdam, Golm, Germany
PLoS Comput Biol 1:e40. 2005..Supplemental material is available in the Plant Subtilase Database (PSDB) (http://csbdb.mpimp-golm.mpg.de/psdb.html), as well as from the CSB.DB (http://csbdb.mpimp-golm.mpg.de)...
- Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine proteaseR S Fuller
Department of Biochemistry, University of California, Berkeley 94720
Proc Natl Acad Sci U S A 86:1434-8. 1989..Mutant enzyme molecules lacking as many as 200 C-terminal residues still retained Ca2+-dependent protease activity and were labeled by 125I-labeled Tyr-Ala-Lys-Arg-chloromethyl ketone...
- Novel subtilase cytotoxin produced by Shiga-toxigenic Escherichia coli induces apoptosis in vero cells via mitochondrial membrane damageGen Matsuura
Department of Molecular Infectiology, Graduate School of Medicine, Chiba University, Chiba, Japan
Infect Immun 77:2919-24. 2009..These data suggested that SubAB induced caspase-dependent apoptosis in Vero cells through mitochondrial membrane damage...
- Apoplastic plant subtilases support arbuscular mycorrhiza development in Lotus japonicusNaoya Takeda
Faculty of Biology, Genetics, University of Munich, Grosshaderner Strasse 2, Martinsried, Germany
Plant J 58:766-77. 2009..Our data indicate a role for these subtilases during the fungal infection process in particular arbuscule development...
- Characterization of the subtilase gene family in tomato (Lycopersicon esculentum Mill.)J Meichtry
Institute of Plant Sciences, ETH Zurich, Switzerland
Plant Mol Biol 39:749-60. 1999..At least one of the subtilases was found to be expressed in each organ analysed. Structural features evident from deduced amino acid sequences are discussed with reference to the related mammalian proprotein convertases...
- Escherichia coli subtilase cytotoxin induces apoptosis regulated by host Bcl-2 family proteins Bax/BakKerrie L May
Research Centre for Infectious Diseases, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, SA, Australia
Infect Immun 78:4691-6. 2010....
- Allele-specific suppression of a defective trans-Golgi network (TGN) localization signal in Kex2p identifies three genes involved in localization of TGN transmembrane proteinsK Redding
Department of Biochemistry, Stanford University, California 94305, USA
Mol Cell Biol 16:6208-17. 1996..soi1 mutants also exhibited a recessive defect in vacuolar protein sorting. Suppressor alleles of S0I2 were dominant. These results suggest that the SOI1 and S0I2 genes encode regulators or components of the TLS recognition machinery...
- Furin at the cutting edge: from protein traffic to embryogenesis and diseaseGary Thomas
Vollum Institute, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
Nat Rev Mol Cell Biol 3:753-66. 2002..This review summarizes various features of furin--its structural and enzymatic properties, intracellular localization, trafficking, substrates, and roles in vivo...
- Detergent proteasesKarl Heinz Maurer
Henkel, Enzyme Technology, Henkelstrasse 67 40191, Duesseldorf, Germany
Curr Opin Biotechnol 15:330-4. 2004Over the past 20 years, the development of subtilisins as typical detergent proteases has employed all the tools of enzyme technology, resulting in a constant flow of new and improved enzymes...
- Plasmodium subtilisin-like protease 1 (SUB1): insights into the active-site structure, specificity and function of a pan-malaria drug targetChrislaine Withers-Martinez
Division of Parasitology, MRC National Institute for Medical Research NIMR, Mill Hill, London NW7 1AA, UK
Int J Parasitol 42:597-612. 2012..Our findings demonstrate that it should be possible to develop 'pan-reactive' drug-like compounds that inhibit SUB1 in all three major human malaria pathogens, enabling production of broad-spectrum antimalarial drugs targeting SUB1...
- Identification of proteases that regulate erythrocyte rupture by the malaria parasite Plasmodium falciparumShirin Arastu-Kapur
Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305, USA
Nat Chem Biol 4:203-13. 2008..These results suggest that two mechanistically distinct proteases function to regulate processing of downstream substrates required for efficient release of parasites from host red blood cells...
- A multifunctional serine protease primes the malaria parasite for red blood cell invasionKonstantinos Koussis
Division of Parasitology, National Institute for Medical Research, London, UK
EMBO J 28:725-35. 2009..We propose that PfSUB1 is a multifunctional processing protease with an essential role in both egress of the malaria merozoite and remodelling of its surface in preparation for erythrocyte invasion...
- The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolutionS C Jain
Department of Chemistry, Rutgers University, 610 Taylor Road, Piscataway, NJ, 08854 8087, USA
J Mol Biol 284:137-44. 1998..The structure determination reported here provides additional information about the nature of the interaction between the subtilisin and propeptide domains in this complex...
- Localization of components involved in protein transport and processing through the yeast Golgi apparatusA Franzusoff
Cellular and Structural Biology Department, University of Colorado Health Sciences Center, Denver 80262
J Cell Biol 112:27-37. 1991..Colocalization of the two antigens, one implicated in protein transport through the Golgi apparatus and the other in processing within a late Golgi compartment, supports the conclusion that we have visualized the yeast Golgi apparatus...
- Expression of recombinant Plasmodium falciparum subtilisin-like protease-1 in insect cells. Characterization, comparison with the parasite protease, and homology modelingChrislaine Withers-Martinez
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom
J Biol Chem 277:29698-709. 2002..homology modeling of the PfSUB-1 catalytic domain based on an alignment with closely related bacterial subtilisins and an orthologue from the rodent malaria Plasmodium yoelii suggests that the protease has at least three ..
- Proprotein and prohormone convertases: a family of subtilases generating diverse bioactive polypeptidesN G Seidah
Laboratories of Biochemical and Molecular Neuroendocrinology and the Protein Engineering Network of Centres of Excellence, Clinical Research Institute of Montreal, Canada
Brain Res 848:45-62. 1999....
- Furin: a mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteinsK Nakayama
Institute of Biological Sciences and Gene Experiment Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305, Japan
Biochem J 327:625-35. 1997..The identification of a novel family of endoproteases homologous with bacterial subtilisins and yeast Kex2p has accelerated progress in understanding the complex mechanisms underlying the production of ..
- The proprotein convertasesD F Steiner
Howard Hughes Medical Institute, University of Chicago, 5841 South Maryland Avenue, MC 1028, Chicago, IL 60637, USA
Curr Opin Chem Biol 2:31-9. 1998....
- Purification and characterization of the subtilisin-like protease of Streptococcus suis that contributes to its virulenceLaetitia Bonifait
Groupe de Recherche en Ecologie Buccale GREB, Faculte de Medecine Dentaire, Universite Laval, Quebec City, Quebec, Canada
Vet Microbiol 148:333-40. 2011..In conclusion, the SspA of S. suis shared similarities with subtilisin-like proteases produced by other pathogenic streptococci and may contribute to the pathogenic process of S. suis infections...
- Subtilase cytotoxin activates PERK, IRE1 and ATF6 endoplasmic reticulum stress-signalling pathwaysJennifer J Wolfson
Division of Geographic Medicine and Infectious Diseases, Tufts New England Medical Center, Boston, MA 02111, USA
Cell Microbiol 10:1775-86. 2008..The rapidity with which ER stress-signalling responses are triggered by exposure of cells to SubAB is consistent with the hypothesis that cleavage by the toxin causes BiP to dissociate from the signalling molecules...
- Failure of ventral closure and axial rotation in embryos lacking the proprotein convertase FurinA J Roebroek
Laboratory for Molecular Oncology, Experimental Genetics Group, Center for Human Genetics, University of Leuven and Flanders Interuniversity Institute for Biotechnology VIB, Herestraat 49, B 3000 Leuven
Development 125:4863-76. 1998..Overall, the present data identify Furin as an important activator of signals responsible for ventral closure and embryonic turning...
- Insights from bacterial subtilases into the mechanisms of intramolecular chaperone-mediated activation of furinUjwal Shinde
Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97229, USA
Methods Mol Biol 768:59-106. 2011Prokaryotic subtilisins and eukaryotic proprotein convertases (PCs) are two homologous protease subfamilies that belong to the larger ubiquitous super-family called subtilases...
- The neuroendocrine precursor 7B2 is a sulfated protein proteolytically processed by a ubiquitous furin-like convertaseL Paquet
Laboratory of Structure and Metabolism of Neuropeptides, Clinical Research Institute of Montreal, University of Montreal, Quebec, Canada
J Biol Chem 269:19279-85. 1994..These results demonstrate that pro7B2 is first processed by a furin-like enzyme within the trans-Golgi network into a 23-kDa form that is then sequestered into secretory granules...
- Subtilase cytotoxin induces apoptosis in HeLa cells by mitochondrial permeabilization via activation of Bax/Bak, independent of C/EBF-homologue protein (CHOP), Ire1alpha or JNK signalingKinnosuke Yahiro
Department of Molecular Infectiology, Graduate School of Medicine, Chiba University, Chuo Ku, Chiba, Japan
Microb Pathog 49:153-63. 2010....
- Subtilase cytotoxin enhances Escherichia coli survival in macrophages by suppression of nitric oxide production through the inhibition of NF-κB activationHiroyasu Tsutsuki
Departments of Molecular Infectiology, Chiba University, Chiba, Japan
Infect Immun 80:3939-51. 2012..coli survival in macrophages. Thus, we hypothesize that SubAB might represent a novel bacterial strategy to circumvent host defense during STEC infection...
- The new allelic variant of the subtilase cytotoxin (subAB2) is common among Shiga toxin-producing Escherichia coli strains from large game animals and their meat and meat productsSergio Sanchez
Unidad de Enterobacterias, Servicio de Bacteriologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 Madrid, Spain
Vet Microbiol 166:645-9. 2013....
- Inhibition of water absorption and selective damage to human colonic mucosa are induced by subtilase cytotoxin produced by Escherichia coli O113:H21Elizabeth Gerhardt
Laboratorio de Fisiopatogenia, Departamento de Fisiologia, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Infect Immun 81:2931-7. 2013..coli O113:H21 expressing only SubAB was assayed. This is the first study showing that SubAB may directly participate in the mechanisms of diarrhea in children infected with non-O157 STEC strains. ..
- Processing of pro-islet amyloid polypeptide (proIAPP) by the prohormone convertase PC2M K Badman
Department of Human Anatomy, University of Oxford, UK
FEBS Lett 378:227-31. 1996..These data are consistent with processing of proIAPP in beta-cell secretory granules. Abnormal cellular proteolysis associated with type 2 diabetes could contribute to IAPP amyloidosis...
- The occurrence of subtilase-cytotoxin-encoding genes in environmental Escherichia coli isolated from a Northern California estuaryMaria Das Gracas C Pereira
Department of Population Health and Reproduction, School of Veterinary Medicine, 2012 Haring Hall, University of California, Davis, CA 95616, USA
Can J Microbiol 59:437-41. 2013..To further understand potential health risks posed by strains encoding SubAB, future epidemiological studies should consider screening isolates for subAB regardless of the presence of Shiga-toxin-encoding genes...
- Subtilase cytotoxin encoding genes are present in human, sheep and deer intimin-negative, Shiga toxin-producing Escherichia coli O128:H2Sergio Sanchez
Laboratorio de Enterobacterias, Servicio de Bacteriologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 Madrid, Spain
Vet Microbiol 159:531-5. 2012..Our results indicate that sheep and deer represent a reservoir of SubAB-positive STEC O128:H2 strains and thus a potential source of human infection...
- A dietary non-human sialic acid may facilitate hemolytic-uremic syndromeJonas C Löfling
Glycobiology Research and Training Center, University of California, La Jolla, San Diego, CA 92093 0687, USA
Kidney Int 76:140-4. 2009....
- Bi-cycling the furin pathway: from TGN localization to pathogen activation and embryogenesisS S Molloy
Vollum Institute, Oregon Health Sciences University, Portland 97201, USA
Trends Cell Biol 9:28-35. 1999....
- Tissue factor–dependent procoagulant activity of subtilase cytotoxin, a potent AB5 toxin produced by shiga toxigenic Escherichia coliHui Wang
Research Centre for Infectious Diseases, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia, Australia
J Infect Dis 202:1415-23. 2010..Our results suggest that the procoagulant effect of SubAB may be dependent on both the up‐regulation of TF expression and the activation of TF by means of BiP cleavage...
- Processing of transforming growth factor beta 1 precursor by human furin convertaseC M Dubois
Department of Pediatrics, Faculty of Medicine, Universite de Sherbrooke, Quebec, Canada
J Biol Chem 270:10618-24. 1995..Collectively, these results provide evidence that in our experimental systems the TGF beta 1 precursor is efficiently and correctly processed by human furin thus permitting release of the biologically active peptide...
- Intracellular trafficking of furin is modulated by the phosphorylation state of a casein kinase II site in its cytoplasmic tailB G Jones
Vollum Institute, Oregon Health Sciences University, Portland 97201 3098, USA
EMBO J 14:5869-83. 1995..Thus, routing of furin is a two-tiered process combining a set of trafficking signals comprised of the primary amino acid sequence of the tail with its phosphorylation state...
- Circular dichroism studies of subtilisin Carlsberg immobilised on micron sized silica particlesAshok Ganesan
Department of Pure and Applied Chemistry, 295 Cathedral Street, University of Strathclyde, Glasgow G1 1XL, UK
Biochim Biophys Acta 1764:1119-25. 2006..Additional evidence for loss of native conformation is provided by the significant decrease in the near UV CD spectrum. These results for the first time clearly demonstrate the origin of enzyme instability in the immobilised state...
- Localization of endogenous furin in cultured cell linesJ Shapiro
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Histochem Cytochem 45:3-12. 1997..Therefore, our results suggest that endogenous furin is predominantly localized to the area of the Golgi complex, most likely within the trans-Golgi network...
- HLA-DQ8 is a predisposing molecule for detergent enzyme subtilisin BPN'-induced hypersensitivityAiling Xue
Department of Immunology, Mayo Clinic, Rochester, MN 55905, USA
Clin Immunol 117:302-15. 2005....
- Improved autoprocessing efficiency of mutant subtilisins E with altered specificity by engineering of the pro-regionM Takahashi
Department of Bioscience, Fukui Prefectural University, Fukui 910 1195, Japan
J Biochem 130:99-106. 2001..Therefore, to enhance processing, we engineered the pro-region of mutant subtilisins E of Bacillus subtilis with altered substrate specificity...
- Testicular expression of PC4 in the rat: molecular diversity of a novel germ cell-specific Kex2/subtilisin-like proprotein convertaseN G Seidah
J A DeSève Laboratories of Biochemical, Clinical Research Institute of Montreal, Quebec, Canada
Mol Endocrinol 6:1559-70. 1992..The stage-specific expression of PC4 in testis indicates its potential role in the developmental maturation of germ cells and that this convertase may play a specific physiological function in reproduction...
- Furin initiates gelsolin familial amyloidosis in the Golgi through a defect in Ca(2+) stabilizationC D Chen
Departments of Molecular and Cell Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA
EMBO J 20:6277-87. 2001..We propose that coincidence of membrane trafficking pathways contributes to the development of proteolysis-initiated amyloid disease...
- Cloning and primary sequence of a mouse candidate prohormone convertase PC1 homologous to PC2, Furin, and Kex2: distinct chromosomal localization and messenger RNA distribution in brain and pituitary compared to PC2N G Seidah
J A DeSève Laboratories of Biochemical, Clinical Research Institute of Montreal, Quebec, Canada
Mol Endocrinol 5:111-22. 1991..The genes coding for mPC1 and mPC2 map to the murine chromosomes 13 (band 13c) and 2 (2F3-2H2 region), respectively...
- Complex between the subtilisin from a mesophilic bacterium and the leech inhibitor eglin-CZ Dauter
European Molecular Biology Laboratory EMBL, Hamburg, Germany
Acta Crystallogr B 47:707-30. 1991..1% including two Ca2+ ions and 312 water molecules. The structure is discussed in terms of its physicochemical properties in solution and its relation to other Bacillus subtilisins.
- Refined crystal structure of the complex of subtilisin BPN' and Streptomyces subtilisin inhibitor at 1.8 A resolutionY Takeuchi
Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd, Yokohama, Japan
J Mol Biol 221:309-25. 1991....
- Refined crystal structures of subtilisin novo in complex with wild-type and two mutant eglins. Comparison with other serine proteinase inhibitor complexesD W Heinz
Pharmaceutical Division, Ciba Geigy Ltd, Basel, Switzerland
J Mol Biol 217:353-71. 1991..Comparison of the complexes showed a rigid body rotation for the eglin c core of 11.5 degrees with respect to the enzyme, probably caused by different intermolecular contacts in both crystal forms...
- Recombining low homology, functionally rich regions of bacterial subtilisins by combinatorial fragment exchangeD Dafydd Jones
School of Biosciences, Cardiff University, Cardiff, United Kingdom
PLoS ONE 6:e24319. 2011..fragment exchange was utilised to recombine key structural and functional low homology regions of bacilli subtilisins to generate new active hybrid proteases with altered substrate profiles...
- SPC4, SPC6, and the novel protease SPC7 are coexpressed with bone morphogenetic proteins at distinct sites during embryogenesisD B Constam
Harvard University, Department of Molecular and Cellular Biology, The Biological Laboratories, Cambridge, Massachusetts 02138, USA
J Cell Biol 134:181-91. 1996..A comparison of these expression patterns with those of several bone morphogenetic proteins (BMPs) indicates that processing of these growth factors may be limited by the local availability of SPCs...
- cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mRNAs encoding candidates for pro-hormone processing proteinasesN G Seidah
J A DeSève Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
DNA Cell Biol 9:415-24. 1990..8 kb) demonstrated tissue and cellular specificity of expression, only within endocrine and neuroendocrine cells. These data suggest that mPC1 and mPC2 represent prime candidates for tissue-specific pro-hormone converting proteinases...
- Structural comparison of two serine proteinase-protein inhibitor complexes: eglin-c-subtilisin Carlsberg and CI-2-subtilisin NovoC A McPhalen
Department of Biochemistry, University of Alberta, Edmonton, Canada
Biochemistry 27:6582-98. 1988..The 24 main-chain plus C beta atoms of P4 to P1' overlap with an rms deviation of 0.19 A. Features contributing to the inhibitory nature of eglin-c and CI-2 are discussed...
- Subtilisins of Bacillus spp. hydrolyze keratin and allow growth on feathersK L Evans
Department of Microbiology, North Carolina State University, Raleigh 27695, USA
Can J Microbiol 46:1004-11. 2000..PWD-1 and the kerA protease efficiently degrade keratin, keratin hydrolysis and growth on feathers is a property that can be conferred by appropriate expression of the major subtilisins, including the industrially produced enzymes.
- Evolution of a subtilisin-like protease gene family in the grass endophytic fungus Epichloë festucaeMichelle K Bryant
Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North, New Zealand
BMC Evol Biol 9:168. 2009..In fungi, SLPs can play either a general nutritive role, or may play specific roles in cell metabolism, or as pathogenicity or virulence factors...
- The NH2-terminal proregion of peptidylglycine alpha-amidating monooxygenase facilitates the secretion of soluble proteinsR E Mains
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Mol Endocrinol 9:3-13. 1995..In both AtT-20 cells and hEK-293 cells, the PAM/PC2 fusion molecule was able to exit the endoplasmic reticulum more rapidly than PC2...
- cDNA structure, tissue distribution, and chromosomal localization of rat PC7, a novel mammalian proprotein convertase closest to yeast kexin-like proteinasesN G Seidah
Clinical Research Institute of Montreal, Canada
Proc Natl Acad Sci U S A 93:3388-93. 1996..The gene for PC7 (Pcsk7) was mapped to mouse chromosome 9 by linkage analysis of an interspecific backcross DNA panel...
- Water dynamics and salt-activation of enzymes in organic media: mechanistic implications revealed by NMR spectroscopyRoss K Eppler
Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 103:5706-10. 2006..This increased mobility may contribute to the dramatic increases in biocatalyst activity...
- Identification of a second human subtilisin-like protease gene in the fes/fps region of chromosome 15M C Kiefer
Chiron Corporation, Emeryville, CA 94608
DNA Cell Biol 10:757-69. 1991..The close proximity of the fur and PACE4 genes on chromosome 15 suggests that these genes probably evolved from a common ancestor by gene duplication...
- Purification and characterization of the prohormone convertase PC1(PC3)Y Zhou
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans 70112
J Biol Chem 268:5615-23. 1993....
- Identification and functional expression of a new member of the mammalian Kex2-like processing endoprotease family: its striking structural similarity to PACE4T Nakagawa
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki
J Biochem 113:132-5. 1993..Transfection experiments revealed that PC6 was capable of cleaving precursors at dibasic sites. These observations suggest that PC6 is a candidate for a processing endoprotease responsible for the maturation of gastrointestinal peptides...
- The engineering of binding affinity at metal ion binding sites for the stabilization of proteins: subtilisin as a test caseM W Pantoliano
Genex Corporation, Gaithersburg, Maryland 20877
Biochemistry 27:8311-7. 1988..8 and 13.2 A, respectively, from the bound Ca2+. These distances and the degree of enhanced binding are consistent with simple electrostatic theory.(ABSTRACT TRUNCATED AT 250 WORDS)..
- alpha1-Antitrypsin Portland, a bioengineered serpin highly selective for furin: application as an antipathogenic agentF Jean
Vollum Institute, Oregon Health Sciences University, Portland, OR 97201, USA
Proc Natl Acad Sci U S A 95:7293-8. 1998..Finally, we propose a structural model of the alpha1-PDX-reactive site loop that explains the high degree of enzyme selectivity of this serpin and which can be used to generate small molecule furin inhibitors...
- Localization of prohormone convertases 1/3 and 2 in the human pituitary gland and pituitary adenomas: analysis by immunohistochemistry, immunoelectron microscopy, and laser scanning microscopyI Takumi
Department of Neurosurgery, Nippon Medical School, Sendagi, Tokyo, Japan
Mod Pathol 11:232-8. 1998..The high incidence of PC1/3 and PC2 in nonfunctioning adenomas might be related to the processing of chromogranin A...
- Ara12 subtilisin-like protease from Arabidopsis thaliana: purification, substrate specificity and tissue localizationJohn M U Hamilton
School of Biological and Biomedical Sciences, University of Durham, South Road, Durham DH1 3LE, UK
Biochem J 370:57-67. 2003..Possible functions of Ara12 are discussed in the light of the involvement of a number of plant subtilisin-like proteases in morphogenesis...
- Eukaryotic protein processing: endoproteolysis of precursor proteinsN G Seidah
Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, QC, Canada
Curr Opin Biotechnol 8:602-7. 1997....
- Structural and biochemical characterization of a fluorogenic rhodamine-labeled malarial protease substrateMichael J Blackman
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, U K
Biochemistry 41:12244-52. 2002..These data also indicate that the rhodamine dimers fluoresce and that the associated lifetimes are subnanosecond...
- Mammalian subtilisin/kexin isozyme SKI-1: A widely expressed proprotein convertase with a unique cleavage specificity and cellular localizationN G Seidah
Laboratory of Biochemical, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, QC, Canada H2W 1R7
Proc Natl Acad Sci U S A 96:1321-6. 1999..In vitro studies suggest that SKI-1 is a Ca2+-dependent serine proteinase exhibiting a wide pH optimum for cleavage of pro-brain-derived neurotrophic factor...
- A subtilisin-like protein in secretory organelles of Plasmodium falciparum merozoitesM J Blackman
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom
J Biol Chem 273:23398-409. 1998..The subcellular location and timing of secretion of p47 suggest that it is likely to play a role in erythrocyte invasion. PfSUB-1 is a new potential target for antimalarial drug development...
- Functional characterization of the propeptide of Plasmodium falciparum subtilisin-like protease-1Letitia Jean
Parasitology and Physical Biochemistry, National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom
J Biol Chem 278:28572-9. 2003..significant secondary structure on its own, comparable with that of folded propeptides of some bacterial subtilisins. Kinetic studies demonstrated that rp31 is a fast binding, high affinity inhibitor of PfSUB-1...
- Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigenS S Molloy
Vollum Institute, Oregon Health Sciences University, Portland 97201
J Biol Chem 267:16396-402. 1992....
- Golgi and vacuolar membrane proteins reach the vacuole in vps1 mutant yeast cells via the plasma membraneS F Nothwehr
Institute of Molecular Biology, University of Oregon, Eugene 97403
J Cell Biol 129:35-46. 1995..We propose a model in which Vps1p is required for formation of vesicles from the late Golgi apparatus that carry vacuolar and Golgi membrane proteins bound for the prevacuolar compartment...
- Two independent targeting signals in the cytoplasmic domain determine trans-Golgi network localization and endosomal trafficking of the proprotein convertase furinW Schafer
Institut fur Virologie, Philipps Universitat Marburg, Germany
EMBO J 14:2424-35. 1995..The data support the concept that the acidic signal, which is the dominant one, retains furin in the TGN, whereas the YKGL motif acts as a retrieval signal for furin that has escaped to the cell surface...
- Structural basis of inhibition revealed by a 1:2 complex of the two-headed tomato inhibitor-II and subtilisin CarlsbergIsabelle H Barrette-Ng
Canadian Institutes for Health Research Group in Protein Structure and Function, Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
J Biol Chem 278:24062-71. 2003..subtilisin)2 complex provides a molecular framework for understanding how multiple inhibitory domains in a single Potato II type proteinase inhibitor molecule from the Potato II family act to inhibit proteolytic enzymes...
- The kex2 gene from the dimorphic and human pathogenic fungus Paracoccidioides brasiliensisEmerson J Venancio
Departamento de Ciências Patológicas, Centro de Ciencias Biologicas, Universidade Estadual de Londrina, 86051 970 Londrina, PR, Brasil
Yeast 19:1221-31. 2002..A putative Golgi retrieval signal (YEFEMI) has also been found in the cytoplasmic tail. The complete nucleotide sequence of Pbkex2 and its flanking regions have been submitted to GenBank database under Accession No. AF486805...
- Retrieval of resident late-Golgi membrane proteins from the prevacuolar compartment of Saccharomyces cerevisiae is dependent on the function of Grd19pW Voos
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
J Cell Biol 140:577-90. 1998..We conclude that Grd19p is a component of the retrieval machinery that functions by direct interaction with the cytosolic tails of certain TGN membrane proteins during the sorting/budding process at the prevacuolar compartment...
- 7B2 is a specific intracellular binding protein of the prohormone convertase PC2S Benjannet
J A DeSève Laboratories of Molecular, Clinical Research Institute of Montreal, Quebec, Canada
J Neurochem 64:2303-11. 1995..Coexpression of 7B2 and PC2, although resulting in an elevation of the level of pro-PC2, did not eliminate the processing of pro-PC2 to PC2.(ABSTRACT TRUNCATED AT 250 WORDS)..
- Purification and characterization of Ak.1 protease, a thermostable subtilisin with a disulphide bond in the substrate-binding cleftH S Toogood
Thermophile Research Unit, University of Waikato, Private Bag 3105, Hamilton 2001, New Zealand
Biochem J 350:321-8. 2000..The protease was originally investigated to determine its usefulness in the clean-up of DNA at high temperatures. However, it was found that this protease has a limited substrate specificity, so this application was not explored further...
- Incomplete processing of proinsulin to insulin accompanied by elevation of Des-31,32 proinsulin intermediates in islets of mice lacking active PC2M Furuta
Department of Biochemistry, University of Chicago, Chicago, Illinois 60637, USA
J Biol Chem 273:3431-7. 1998....
- Precursor processing by kex2/furin proteasesNathan C Rockwell
Department of Biological Chemistry, University of Michigan Medical Center, Room 5413 Med Sci I, 1301 East Catherine, Ann Arbor Michigan 48109, USA
Chem Rev 102:4525-48. 2002
- Comparative biosynthesis, covalent post-translational modifications and efficiency of prosegment cleavage of the prohormone convertases PC1 and PC2: glycosylation, sulphation and identification of the intracellular site of prosegment cleavage of PC1 and PS Benjannet
J A DeSève Laboratory of Molecular Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada
Biochem J 294:735-43. 1993..In order to test the possibility that zymogen processing is performed by furin, we co-expressed this convertase with either pro-PC1 or pro-PC2. The data demonstrated the inability of furin to cleave either proenzyme...
- Bioactivation of Müllerian inhibiting substance during gonadal development by a kex2/subtilisin-like endoproteaseM W Nachtigal
Department of Physiology, Program in Developmental Biology, University of California, San Francisco 94143 0444, USA
Proc Natl Acad Sci U S A 93:7711-6. 1996..Our results suggest that MIS is a natural substrate for PC5, thereby supporting a role for prohormone convertases in the activation of transforming growth factor beta-related hormones during development...
- cDNA structure of the mouse and rat subtilisin/kexin-like PC5: a candidate proprotein convertase expressed in endocrine and nonendocrine cellsJ Lusson
J A DeSève Laboratorie of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, PQ, Canada
Proc Natl Acad Sci U S A 90:6691-5. 1993..In situ hybridization of rat brain sections demonstrated a unique distribution of PC5 compared to PC1, PC2, and furin...
- Intracellular trafficking and activation of the furin proprotein convertase: localization to the TGN and recycling from the cell surfaceS S Molloy
Vollum Institute, Portland, OR
EMBO J 13:18-33. 1994..Finally, we show that proregion removal is necessary but not sufficient for enzyme activation...
- A subtilisin-like serine protease is required for epidermal surface formation in Arabidopsis embryos and juvenile plantsH Tanaka
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
Development 128:4681-9. 2001....
- Subtilisin-like proteases of the malaria parasiteChrislaine Withers-Martinez
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
Mol Microbiol 53:55-63. 2004..They may have potential as novel drug targets. Here, we review progress in our understanding of the maturation, specificity, structure and function of these Plasmodium subtilases...
- The prosegment-subtilisin BPN' complex: crystal structure of a specific 'foldase'T Gallagher
Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, USA
Structure 3:907-14. 1995..Mature subtilisin represents a class of proteins that lacks an efficient folding pathway. Refolding of mature SBT is extremely slow unless catalyzed by the independently expressed prosegment, leading to a bimolecular complex...
- Reconstructing the diversification of subtilisins in the pathogenic fungus Metarhizium anisopliaeSavita Bagga
Department of Entomology, University of Maryland, 4112 Plant Science Building, College Park, MD 20742 4454, USA
Gene 324:159-69. 2004..Here we sought a global characterization of the diversity of subtilisins in the insect pathogen Metarhizium anisopliae...
- Functional analysis of the propeptides of subtilisin E and aqualysin I as intramolecular chaperonesH Takagi
Department of Bioscience, Fukui Prefectural University, Japan
FEBS Lett 508:210-4. 2001..Further, some enzymatic properties of some chimeras in which the subtilisin mature domain is partly swapped with that of aqualysin I were shown to be more similar to those of aqualysin I...
- Structural basis of thermostability. Analysis of stabilizing mutations in subtilisin BPN'Orna Almog
Department of Clinical Biochemistry, Faculty of Health Sciences, Ben Gurion University, Beer Sheva 84105, Israel
J Biol Chem 277:27553-8. 2002..The effects are attributed to the new disulfide cross-link and to improved hydrophobic packing, new hydrogen bonds, and other rearrangements in the N-terminal region...
- A protein-based therapeutic for human cytomegalovirus infectionF Jean
Vollum Institute, and Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, 3181 Southwest Sam Jackson Park Road, Portland, OR 97201, USA
Proc Natl Acad Sci U S A 97:2864-9. 2000....
- Gene targeting demonstrates that the Plasmodium berghei subtilisin PbSUB2 is essential for red cell invasion and reveals spontaneous genetic recombination eventsPierrick Uzureau
Unité de Biologie des Interactions Hôte Parasite, Institut Pasteur CNRS URA 2581, 25 rue du Dr Roux, 75015 Paris, France
Cell Microbiol 6:65-78. 2004..berghei...
- Maturation of the trans-Golgi network protease furin: compartmentalization of propeptide removal, substrate cleavage, and COOH-terminal truncationM Vey
Institut f Virologie, Philipps Universitat Marburg, Germany
J Cell Biol 127:1829-42. 1994..This observation supports the concept that secretion of truncated furin is a physiological event that may have important implications for the processing of extracellular substrates...
- Engineering subtilisin into a fluoride-triggered processing protease useful for one-step protein purificationBiao Ruan
Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
Biochemistry 43:14539-46. 2004..Cleavage can be triggered by the addition of fluoride to release the pure target protein. The column is then regenerated by stripping off the tightly bound prodomain at pH 2.1. Ten proteins have been purified to date by this method...
- A new subfamily of fungal subtilases: structural and functional analysis of a Pleurotus ostreatus memberVincenza Faraco
Dipartimento di Chimica Organica e Biochimica, Universita di Napoli Federico II, Complesso Universitario Monte S Angelo, Via Cinthia, 80126 Napoli, Italy
Microbiology 151:457-66. 2005..A new subgroup of subtilisin-like proteases, belonging to the pyrolysin family, has been defined, which includes proteases from ascomycete and basidiomycete fungi...
- Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutantW J Luo
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
J Cell Biol 138:731-46. 1997..Thus, cell surface delivery of mutant Pma1 can occur as a consequence of disturbances at several different sites in the endosomal system...
- Phylogenetic and exon-intron structure analysis of fungal subtilisins: support for a mixed model of intron evolutionChengshu Wang
School of Biological Sciences, University of Wales Swansea, Swansea SA2 8PP, UK
J Mol Evol 60:238-46. 2005Phylogenetic and exon-intron structure analyses of intra- and interspecific fungal subtilisins in this study provided support for a mixed model of intron evolution: a synthetic theory of introns-early and introns-late speculations...
- Blockade of Anthrax Cytotoxicity Using Furin InhibitorsIris Lindberg; Fiscal Year: 2003..this cleavage is performed by a cellular surface enzyme known as furin, a member of the family of eukaryotic subtilisins. This application is directed toward the idea that the cytotoxic action of anthrax toxin can be attenuated ..
- PROTEIN FOLDING AND STABILITY OF SUBTILISINPhilip Bryan; Fiscal Year: 2004..Understanding the relationships between facile folding and stability should advance the fields of protein engineering, protein structure prediction and de novo protein design. ..
- NEUTRON STUDIES OF PROTEIN DYNAMICS AND SOLVATIONAnthony Kossiakoff; Fiscal Year: 1991..A number of mutant subtilisins have been crystallized...
- Proprotein Processing, Trafficking and Secretion Gordon ConferenceNabil Seidah; Fiscal Year: 2006..The integration of protease systems that are required both for normal physiology and human disease will be relevant to our understanding of the molecular bases of diabetes, cancer, Alzheimer's and drug addiction. ..
- ProTRH sorting to the regulated secretory pathwayEDUARDO NILLNI; Fiscal Year: 2006..The sorting of the proTRH N- and C-terminal fragments is routed to two different pathways. Aim 4. The tertiary structure of proTRH contributes to its processing by PC1 and PC2 and further sorting to the RSP. ..
- PROTRH GENE TRANSCRIPTION AND BIOSYNTHESES BY LEPTINEDUARDO NILLNI; Fiscal Year: 2008..B) Since the melanocortin system represents the indirect pathway of action on TRH neurons, we will also determine whether alpha- MSH affects the biosynthesis, maturation and activity of PCI, PC2, proSAAS, 7B2 and CPE. ..
- Impact of Sleep on Feeding and the Metabolic SyndromeJoseph Bass; Fiscal Year: 2006..Insight gained from these studies will provide new strategies to prevent metabolic complications associated with sleep deprivation and uncover novel metabolic targets for treatment of obesity and its co-morbidities. ..
- VP peptides and receptors in diagnosis of breast cancer.WILLIAM NORTH; Fiscal Year: 2006..Another successful end-point would be the finding that these same methods can successfully monitor current treatments, and potentially lead to new and more effective ones. ..
- THE CRES GENE IN REPRODUCTIONGail Cornwall; Fiscal Year: 2007..Also, the newly purchased mass spec will enable on site protein analysis for the studies described above. In short, the environment at TTUHSC is exceptional and will fully support my proposed studies. ..
- MOLECULAR TOXICOLOGY IN HUMAN KIDNEY CELLSLawrence Lash; Fiscal Year: 2007..Achievement of these aims should build on our previous work in human kidney cells and extend it to provide a much more complete understanding of the various and complex ways in which DCVC affects the human kidney [unreadable] [unreadable]..
- REGULATION OF EXTRACELLULAR SUPEROXIDE DISMUTASE BY PROTRussell Bowler; Fiscal Year: 2004..This will not only define new aspects of the pathogenesis and regulation of vascular tone and inflammation, but can open new approaches to pharmacologically control these events. ..
- NEUROPEPTIDES AND SMALL CELL CARCINOMAWILLIAM NORTH; Fiscal Year: 2002..The information obtained is expected to result in effective chemotherapeutic and immunotherapeutic interventions for all patients with limited and extensive SCCL. ..
- GRSA Proteins in the Diagnosis of Breast CancerWILLIAM NORTH; Fiscal Year: 2002..These methods could be applicable to all patients with breast cancer or suspected breast cancer. ..
- MOLECULAR COMPONENENTS OF INSULIN RECEPTOR BIOGENESISJoseph Bass; Fiscal Year: 2003..Steiner, in addition to Drs. Graeme Bell, Susan Lindquist and other excellent scientists at the University of Chicago will promote his continued development as an independent researcher. ..
- GLUTATHIONE AND MITOCHONDRIA IN TOXIC RENAL INJURYLawrence Lash; Fiscal Year: 2003..This information may have therapeutic applications for prevention of renal cellular injury or for understanding mitochondrial diseases or age-related decreases that occur in mitochondrial function. ..
- MOLECULAR COMPONENTS OF INSULIN RECEPTOR BIOGENESISJoseph Bass; Fiscal Year: 2003..abstract_text> ..
- Keratinocyte Costimulation and Th2-Cell Immune DeviationAnthony Gaspari; Fiscal Year: 2009..Similar mechanistic studies of the molecular basis of other skin diseases such as psoriasis have led to the development of biological response modifiers that are now in clinical use. ..
- GENETIC RESCUE OF PRO-LHRH PROCESSING IN CPE FAT MICEWilliam Wetsel; Fiscal Year: 2002....
- Role of HCMV Glycoprotein in Viral BiogenesisGary Thomas; Fiscal Year: 2005..Together, these studies will increase our knowledge of the cell biology of HCMV gB and provide insight into the molecular mechanisms of viral assembly that will enable development of novel therapeutics to treat viral disease. ..
- THE CRES GENE IN REPRODUCTIONGail Cornwall; Fiscal Year: 2005..abstract_text> ..
- MOLECULAR ANALYSIS OF T. DENTICOLA-HOST INTERACTIONSJ Fenno; Fiscal Year: 2004....
- Micellar VIP Nanoparticles for Rheumatoid ArthritisIsrael Rubinstein; Fiscal Year: 2007..abstract_text> ..
- CCK in Generating Enzyme - Structure, Location and RoleMargery Beinfeld; Fiscal Year: 2005..The effect of this inhibition on the processing of pro CCK will be determined. ..
- DDR1 controls tumor homing in breast to bone metastasisCelso Gomez Sanchez; Fiscal Year: 2004..A survey of the CRISP database showed that the NIH currently funds no other research on DDR1. ..
- Role of PACS proteins in polycystin-2 trafficking and ADPKDGary Thomas; Fiscal Year: 2010....
- Role of PACS-1 in HIV-1 ImmunoevasionGary Thomas; Fiscal Year: 2007..Elucidating how this Nef-induced signaling pathway is used by HIV-1 will provide better understanding of the biochemical basis underlying HIV-1 pathogenesis and will identify new targets to combat this deadly virus. ..
- IRRITANTS EFFECTS ON EPIDERMAL ANTIGEN PRESENTATIONAnthony Gaspari; Fiscal Year: 2002....