plasmin

Summary

Summary: A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.

Top Publications

  1. ncbi Structure and function of the plasminogen/plasmin system
    Francis J Castellino
    W M Keck Center for Transgene Research, Department of Chemistry and Biochemistry, University of Notre Dame, IN 46556, USA
    Thromb Haemost 93:647-54. 2005
  2. ncbi Cleavage of proBDNF by tPA/plasmin is essential for long-term hippocampal plasticity
    Petti T Pang
    Section on Neural Development and Plasticity, Laboratory of Cellular and Synaptic Neurophysiology, National Institute of Child Health and Human Development NICHD, Bethesda, MD 20892, USA
    Science 306:487-91. 2004
  3. ncbi The axonally secreted serine proteinase inhibitor, neuroserpin, inhibits plasminogen activators and plasmin but not thrombin
    T Osterwalder
    Institute of Biochemistry, University of Zurich, Switzerland
    J Biol Chem 273:2312-21. 1998
  4. ncbi Molecular mechanisms involved in the resistance of fibrin to clot lysis by plasmin in subjects with type 2 diabetes mellitus
    E J Dunn
    Academic Unit of Molecular Vascular Medicine, Leeds Institute for Genetics, Health and Therapeutics, University of Leeds, Clarendon Way, Leeds LS1 3EX, UK
    Diabetologia 49:1071-80. 2006
  5. ncbi Urokinase-generated plasmin activates matrix metalloproteinases during aneurysm formation
    P Carmeliet
    Centre for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, University of Leuven, Belgium
    Nat Genet 17:439-44. 1997
  6. pmc The interaction of Streptococcus pneumoniae with plasmin mediates transmigration across endothelial and epithelial monolayers by intercellular junction cleavage
    Cécile Attali
    Institut de Biologie Structurale Jean Pierre Ebel, UMR 5075, Laboratoire d Ingénierie des Macromolécules, 41 rue Jules Horowitz, 38027 Grenoble, France
    Infect Immun 76:5350-6. 2008
  7. ncbi Plasmin activity is required for myogenesis in vitro and skeletal muscle regeneration in vivo
    Monica Suelves
    Centre d Oncologia Molecular, Institut de Recerca Oncologica, Barcelona, Spain
    Blood 99:2835-44. 2002
  8. ncbi uPA/plasmin system-mediated MMP-9 activation is implicated in bronchial epithelial cell migration
    C Legrand
    Laboratoire Pol Bouin-CHU, , IFR 53, and, Reims, France
    Exp Cell Res 264:326-36. 2001
  9. ncbi Identification of a novel plasmin(ogen)-binding motif in surface displayed alpha-enolase of Streptococcus pneumoniae
    Simone Bergmann
    Department of Microbial Pathogenicity, GBF German Research Centre for Biotechnology, 38124 Braunschweig, Germany
    Mol Microbiol 49:411-23. 2003
  10. ncbi Characterization of the interaction between tumor necrosis factor-stimulated gene-6 and heparin: implications for the inhibition of plasmin in extracellular matrix microenvironments
    David J Mahoney
    Medical Research Council Immunochemistry Unit, University of Oxford, United Kingdom
    J Biol Chem 280:27044-55. 2005

Detail Information

Publications322 found, 100 shown here

  1. ncbi Structure and function of the plasminogen/plasmin system
    Francis J Castellino
    W M Keck Center for Transgene Research, Department of Chemistry and Biochemistry, University of Notre Dame, IN 46556, USA
    Thromb Haemost 93:647-54. 2005
    ..system is dependent on the conversion of the plasma zymogen, plasminogen (Pg), to the serine protease plasmin (Pm) by the physiological activators urokinase-type Pg activator (uPA) or tissue-type plasminogen activator (tPA)...
  2. ncbi Cleavage of proBDNF by tPA/plasmin is essential for long-term hippocampal plasticity
    Petti T Pang
    Section on Neural Development and Plasticity, Laboratory of Cellular and Synaptic Neurophysiology, National Institute of Child Health and Human Development NICHD, Bethesda, MD 20892, USA
    Science 306:487-91. 2004
    ..Here we report that tPA, by activating the extracellular protease plasmin, converts the precursor proBDNF to the mature BDNF (mBDNF), and that such conversion is critical for L-LTP ..
  3. ncbi The axonally secreted serine proteinase inhibitor, neuroserpin, inhibits plasminogen activators and plasmin but not thrombin
    T Osterwalder
    Institute of Biochemistry, University of Zurich, Switzerland
    J Biol Chem 273:2312-21. 1998
    ..Although neuroserpin bound and inactivated plasminogen activators and plasmin, no interaction was observed with thrombin...
  4. ncbi Molecular mechanisms involved in the resistance of fibrin to clot lysis by plasmin in subjects with type 2 diabetes mellitus
    E J Dunn
    Academic Unit of Molecular Vascular Medicine, Leeds Institute for Genetics, Health and Therapeutics, University of Leeds, Clarendon Way, Leeds LS1 3EX, UK
    Diabetologia 49:1071-80. 2006
    ....
  5. ncbi Urokinase-generated plasmin activates matrix metalloproteinases during aneurysm formation
    P Carmeliet
    Centre for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, University of Leuven, Belgium
    Nat Genet 17:439-44. 1997
    ..MMPs are secreted as inactive zymogens (pro-MMPs), requiring activation in the extracellular compartment. Plasmin, generated from the zymogen plasminogen by tissue-type plasminogen activator (t-PA) or urokinase-type plasminogen ..
  6. pmc The interaction of Streptococcus pneumoniae with plasmin mediates transmigration across endothelial and epithelial monolayers by intercellular junction cleavage
    Cécile Attali
    Institut de Biologie Structurale Jean Pierre Ebel, UMR 5075, Laboratoire d Ingénierie des Macromolécules, 41 rue Jules Horowitz, 38027 Grenoble, France
    Infect Immun 76:5350-6. 2008
    ..We observed that coating of S. pneumoniae R6 strain with plasminogen or inactivated plasmin increased adherence to pulmonary epithelial A549 and vascular endothelial EaHy cells in vitro...
  7. ncbi Plasmin activity is required for myogenesis in vitro and skeletal muscle regeneration in vivo
    Monica Suelves
    Centre d Oncologia Molecular, Institut de Recerca Oncologica, Barcelona, Spain
    Blood 99:2835-44. 2002
    b>Plasmin, the primary fibrinolytic enzyme, has a broad substrate spectrum and is implicated in biologic processes dependent upon proteolytic activity, such as tissue remodeling and cell migration...
  8. ncbi uPA/plasmin system-mediated MMP-9 activation is implicated in bronchial epithelial cell migration
    C Legrand
    Laboratoire Pol Bouin-CHU, , IFR 53, and, Reims, France
    Exp Cell Res 264:326-36. 2001
    To examine the effects of the uPA/plasmin system on cell migration in relation to the activation of MMP-9, we used ex vivo and in vitro wound-repair models of human bronchial epithelial cells and videomicroscopy techniques that make ..
  9. ncbi Identification of a novel plasmin(ogen)-binding motif in surface displayed alpha-enolase of Streptococcus pneumoniae
    Simone Bergmann
    Department of Microbial Pathogenicity, GBF German Research Centre for Biotechnology, 38124 Braunschweig, Germany
    Mol Microbiol 49:411-23. 2003
    The interaction of Streptococcus pneumoniae with human plasmin(ogen) represents a mechanism to enhance bacterial virulence by capturing surface-associated proteolytic activity in the infected host...
  10. ncbi Characterization of the interaction between tumor necrosis factor-stimulated gene-6 and heparin: implications for the inhibition of plasmin in extracellular matrix microenvironments
    David J Mahoney
    Medical Research Council Immunochemistry Unit, University of Oxford, United Kingdom
    J Biol Chem 280:27044-55. 2005
    ..We also show that heparin can modify another property of the Link module, i.e. its potentiation of the anti-plasmin activity of inter-alpha-inhibitor (IalphaI)...
  11. ncbi Analysis of the invasion-metastasis mechanism in pancreatic cancer: involvement of plasmin(ogen) cascade proteins in the invasion of pancreatic cancer cells
    Xiaodong Tan
    Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860 8556, Japan
    Int J Oncol 28:369-74. 2006
    To clarify the potential involvement of plasmin(ogen) cascade proteins in the cell dissociation and subsequent invasion of pancreatic cancer cells, western blot analysis, immunocytochemistry, immunohistochemistry, and in vitro invasion ..
  12. ncbi Proteolytic activation of monocyte chemoattractant protein-1 by plasmin underlies excitotoxic neurodegeneration in mice
    John J Sheehan
    Department of Pharmacological Sciences and Program in Molecular and Cellular Pharmacology, Stony Brook University, Stony Brook, New York 11794 8651, USA
    J Neurosci 27:1738-45. 2007
    ..Mice deficient in the serine protease plasmin exhibit decreased microglial migration to the site of excitatory neurotransmitter release and are resistant to ..
  13. ncbi Crystal structure of the catalytic domain of human plasmin complexed with streptokinase
    X Wang
    Crystallography Program, Oklahoma Medical Research Foundation, 825 N E 13th Street, Oklahoma City, OK 73104, USA
    Science 281:1662-5. 1998
    ..Complexes of streptokinase with human plasminogen can hydrolytically activate other plasminogen molecules to plasmin, which then dissolves blood clots...
  14. pmc PfbA, a novel plasmin- and fibronectin-binding protein of Streptococcus pneumoniae, contributes to fibronectin-dependent adhesion and antiphagocytosis
    Masaya Yamaguchi
    Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, Suita, Osaka 565 0871, Japan
    J Biol Chem 283:36272-9. 2008
    ..motif in the whole-genome sequence of Streptococcus pneumoniae and identified one, which we called PfbA (plasmin- and fibronectin-binding protein A), that bound to human serum proteins...
  15. ncbi Mini-plasmin found in the epithelial cells of bronchioles triggers infection by broad-spectrum influenza A viruses and Sendai virus
    M Murakami
    Division of Enzyme Chemistry, Institute for Enzyme Research, University of Tokushima, Japan
    Eur J Biochem 268:2847-55. 2001
    ..This protease was extracted from the membrane fraction of rat lungs, purified and then identified as a mini-plasmin. Mini-plasmin was distributed predominantly in the epithelial cells of the upward divisions of bronchioles and ..
  16. ncbi Metastasis-associated protein S100A4 induces angiogenesis through interaction with Annexin II and accelerated plasmin formation
    Alexandre Semov
    Supratek Pharma Inc, Montreal, Quebec H9S 1A9, Canada
    J Biol Chem 280:20833-41. 2005
    ..A synthetic peptide corresponding to the N terminus of annexin II prevented S100A4-induced plasmin formation in the endothelial cell culture...
  17. ncbi The peptide chains of human plasmin. Mechanism of activation of human plasminogen to plasmin
    K C Robbins
    J Biol Chem 242:2333-42. 1967
  18. ncbi Neuronal death in the hippocampus is promoted by plasmin-catalyzed degradation of laminin
    Z L Chen
    Department of Pharmacology, University at Stony Brook, New York 11794 8651, USA
    Cell 91:917-25. 1997
    ..provoke neuronal death in the hippocampus, and the extracellular proteases tissue plasminogen activator (tPA) and plasmin (ogen) have been implicated in this death...
  19. ncbi RNA interference-mediated silencing of the S100A10 gene attenuates plasmin generation and invasiveness of Colo 222 colorectal cancer cells
    Libo Zhang
    Cancer Biology Research Group, Departments of Biochemistry and Molecular Biology and Oncology, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 279:2053-62. 2004
    ..of extracellular S100A10 protein and correlated with a 45% loss of plasminogen binding, a 65% loss in cellular plasmin generation and a complete loss in plasminogen-dependent cellular invasiveness...
  20. pmc Plasmin activates the lymphangiogenic growth factors VEGF-C and VEGF-D
    Bradley K McColl
    Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Melbourne, Victoria 3050, Australia
    J Exp Med 198:863-8. 2003
    ..Here, we report that the serine protease plasmin cleaved both propeptides from the VEGF homology domain of human VEGF-D and thereby generated a mature form ..
  21. ncbi The serine protease plasmin triggers expression of MCP-1 and CD40 in human primary monocytes via activation of p38 MAPK and janus kinase (JAK)/STAT signaling pathways
    Ladislav Burysek
    Department of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, D 89081 Ulm, Germany
    J Biol Chem 277:33509-17. 2002
    The mechanism of proinflammatory activation of human monocytes by plasmin is unknown...
  22. pmc Plasmin potentiates synaptic N-methyl-D-aspartate receptor function in hippocampal neurons through activation of protease-activated receptor-1
    Guido Mannaioni
    Dipartimento di Farmacologia, Universita degli Studi di Firenze, Firenze, Italy
    J Biol Chem 283:20600-11. 2008
    Protease-activated receptor-1 (PAR1) is activated by a number of serine proteases, including plasmin. Both PAR1 and plasminogen, the precursor of plasmin, are expressed in the central nervous system...
  23. ncbi Binding of Candida albicans enolase to plasmin(ogen) results in enhanced invasion of human brain microvascular endothelial cells
    Ambrose Y Jong
    Division of Hematology Oncology, Mailstop 57, Childrens Hospital Los Angeles, Los Angeles, CA 90027, USA
    J Med Microbiol 52:615-22. 2003
    ..albicans enolase to human plasminogen/plasmin was investigated. C...
  24. ncbi An extracellular proteolytic cascade promotes neuronal degeneration in the mouse hippocampus
    S E Tsirka
    Department of Pharmacology, University Medical Center at Stony Brook, New York 11794 8651, USA
    J Neurosci 17:543-52. 1997
    ..Infusion of inhibitors of the extracellular tPA/plasmin proteolytic cascade into the hippocampus protects neurons against excitotoxic injury, suggesting a novel strategy ..
  25. ncbi The reaction between plasmin and C1-inhibitor results in plasmin inhibition by the serpin mechanism
    E W Brown
    Department of Oral Medicine and Diagnostic Sciences, and Center for Molecular Biology of Oral Diseases, University of Illinois at Chicago, 60612, USA
    Blood Coagul Fibrinolysis 13:711-4. 2002
    ..of plasma kallikrein and C1, but also has inhibitory activity against numerous other plasma proteinases such as plasmin. The relevance of plasmin inhibition by the C1-inhibitor has been debated, with some evidence showing that ..
  26. pmc The hippocampal laminin matrix is dynamic and critical for neuronal survival
    Zu Lin Chen
    Laboratory of Neurobiology and Genetics, The Rockefeller University, New York, New York 10021, USA
    Mol Biol Cell 14:2665-76. 2003
    ..The disruption of the laminin layer by laminin-1 1) did not require the intact protein because infusion of plasmin-digested laminin-1 gave similar results; 2) was posttranscriptional, because there was no effect on laminin mRNA ..
  27. ncbi Plasma plasmin-alpha2-plasmin inhibitor complex levels are increased in systemic sclerosis patients with pulmonary hypertension
    M Jinnin
    Department of Dermatology, Faculty of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Japan
    Rheumatology (Oxford) 42:240-3. 2003
    To determine the frequency and clinical significance of plasma plasmin-alpha(2)-plasmin inhibitor complex (PIC) in patients with systemic sclerosis (SSc).
  28. ncbi Enhanced fibrin remodeling in vitro with TGF-beta1, insulin and plasmin for improved tissue-equivalents
    M R Neidert
    Department of Chemical Engineering and Materials Science and Biomedical Engineering, University of Minnesota, Minneapolis 55455, USA
    Biomaterials 23:3717-31. 2002
    ..The optimal condition involved a combination of all three factors investigated-TGF-beta, insulin, plasmin-with medium replacement three times a week...
  29. ncbi Plasmin-mediated activation of platelets occurs by cleavage of protease-activated receptor 4
    Todd M Quinton
    Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 279:18434-9. 2004
    The activation of plasmin from its circulating precursor plasminogen is the mechanism of several clot-busting drugs used to clinically treat patients who have suffered a stroke; however, plasmin thus generated has been shown to activate ..
  30. pmc Plasmin therapy enhances mobilization of HPCs after G-CSF
    Marc Tjwa
    Vesalius Research Center, Leuven, Belgium
    Blood 112:4048-50. 2008
    ..Here we report that genetic loss of the plasminogen activator inhibitor Pai-1 or of the plasmin inhibitor alpha2-antiplasmin increases HPC mobilization in response to G-CSF...
  31. pmc A novel mechanism of plasmin-induced mitogenesis in fibroblasts
    S K Mandal
    Biomedical Research, The University of Texas Health Center at Tyler, Tyler, TX 75708, USA
    J Thromb Haemost 3:163-9. 2005
    The plasminogen activator/plasmin system is believed to play an important role in diverse pathophysiological processes, including wound healing, vascular remodeling and pulmonary fibrosis...
  32. ncbi Plasmin activates pro-matrix metalloproteinase-2 with a membrane-type 1 matrix metalloproteinase-dependent mechanism
    Sara Monea
    Department of Surgery, S A Localio Laboratory for General Surgery Research, New York, New York, USA
    J Cell Physiol 192:160-70. 2002
    ..We previously reported that plasmin treatment of cells results in proMMP-2 activation and increased type IV collagen degradation...
  33. pmc Increased plasminogen binding is associated with metastatic breast cancer cells: differential expression of plasminogen binding proteins
    M Ranson
    Department of Biological Sciences, University of Wollongong, NSW Australia
    Br J Cancer 77:1586-97. 1998
    ..more cell-surface plasminogen in a lysine-dependent manner and (2) was capable of generating large amounts of plasmin compared with the non-metastatic cell lines MCF-7 and T-47D...
  34. ncbi Phospholipid-associated annexin A2-S100A10 heterotetramer and its subunits: characterization of the interaction with tissue plasminogen activator, plasminogen, and plasmin
    Travis J MacLeod
    Cancer Biology Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 278:25577-84. 2003
    ..AIIt or its annexin A2 subunit and the ligands, tissue-type plasminogen activator (t-PA), plasminogen, and plasmin. AIIt bound t-PA (Kd = 0.68 microm), plasminogen (Kd = 0...
  35. ncbi Plasmin generation dependent on alpha-enolase-type plasminogen receptor is required for myogenesis
    Roser Lopez-Alemany
    Institut de Recerca Oncològia, Centre d Oncologia Molecular, L Hospitalet de Llobregat, Barcelona, Spain
    Thromb Haemost 90:724-33. 2003
    b>Plasmin is a potent extracellular protease specialized in the degradation of fibrin (fibrinolysis)...
  36. ncbi Plasmin/plasminogen system in colorectal cancer
    David H Berger
    Michael E DeBakey Department of Surgery, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    World J Surg 26:767-71. 2002
    ..One of the main protease systems involved in tumor cell invasion and metastasis is the plasminogen/plasmin system (PPS)...
  37. ncbi TNF/IL-1-inducible protein TSG-6 potentiates plasmin inhibition by inter-alpha-inhibitor and exerts a strong anti-inflammatory effect in vivo
    H G Wisniewski
    Department of Microbiology, Kaplan Cancer Center, New York University Medical Center, NY 10016, USA
    J Immunol 156:1609-15. 1996
    ..In this work, we show that TSG-6 potentiates the inhibitory effect of l alpha l on the protease activity of plasmin. The plasmin/plasminogen activator system is important in the protease network associated with inflammation...
  38. pmc TGF-beta1 + EGF-initiated invasive potential in transformed human keratinocytes is coupled to a plasmin/MMP-10/MMP-1-dependent collagen remodeling axis: role for PAI-1
    Cynthia E Wilkins-Port
    Center for Cell Biology and Cancer Research, Albany Medical College, Albany, New York 12208, USA
    Cancer Res 69:4081-91. 2009
    ..significant increase in matrix metalloproteinase (MMP)-10 expression and involved a proteolytic axis composed of plasmin, MMP-10, and MMP-1. Neutralization of any one component in this cascade inhibited collagen gel lysis...
  39. ncbi Autologous plasmin for pharmacologic vitreolysis prepared 1 hour before surgery
    Stanislao Rizzo
    Eye Surgery Clinic, Department of Immunohaematology and Transfusion Medicine, Santa Chiara Hospital, Pisa, Italy
    Retina 26:792-6. 2006
    To evaluate the safety and efficacy of intravitreal injection of autologous plasmin enzyme (APE) in inducing a posterior vitreous detachment (PVD). APE was obtained by a modified method 1 hour before surgery.
  40. ncbi Novel aspects and new roles for the serine protease plasmin
    T Syrovets
    Department of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, Helmholtzstr 20, 89081 Ulm, Germany
    Cell Mol Life Sci 61:873-85. 2004
    The serine protease plasmin is distributed throughout the human body in the form of the zymogen plasminogen...
  41. ncbi Plasmin triggers cytokine induction in human monocyte-derived macrophages
    Qun Li
    Institute of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, Helmholtzstr 20, D 89081 Ulm, Germany
    Arterioscler Thromb Vasc Biol 27:1383-9. 2007
    Fibrinolytic activity is upregulated in atherosclerotic lesions, yet little is known about the role of plasmin in macrophage function. We postulated a direct effect of plasmin on human monocyte-derived macrophages.
  42. pmc Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids. Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation
    Anna Tanka-Salamon
    Department of Medical Biochemistry, Semmelweis University, Puskin u 9, Budapest, Hungary
    FEBS J 275:1274-82. 2008
    Thrombi, which are dissolved primarily by plasmin (EC 3.4.21.7.), contain up to millimolar concentrations of fatty acids and these are known to affect the action of the protease...
  43. ncbi Plasmin is involved in inflammation via protease-activated receptor-1 activation in human dental pulp
    Naoto Kamio
    Department of Endodontics, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271 8587, Japan
    Biochem Pharmacol 75:1974-80. 2008
    b>Plasmin is a proteolytic enzyme produced from plasminogen by plasminogen activators. We investigated the function of plasmin in human dental pulp fibroblast-like cells...
  44. ncbi Adhesion or plasmin regulates tyrosine phosphorylation of a novel membrane glycoprotein p80/gp140/CUB domain-containing protein 1 in epithelia
    Tod A Brown
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    J Biol Chem 279:14772-83. 2004
    ..Identity was confirmed by digestion and phosphorylation studies with recombinant gp140-GFP. Plasmin, a serum protease, also converts gp140 to p80, providing biological significance to the cleavage in wounds...
  45. ncbi Acute tissue damage after injections of thrombin and plasmin into rat striatum
    M Xue
    Department of Pathology, Manitoba Institute of Child Health, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    Stroke 32:2164-9. 2001
    ..5 U/5 microL) plasminogen, and low- (0.335 U/5 microL) and high-dose (3.35 U/5 microL) plasmin. Forty-eight hours later rats were perfusion fixed...
  46. ncbi Identification of the annexin A2 heterotetramer as a receptor for the plasmin-induced signaling in human peripheral monocytes
    Yves Laumonnier
    Department of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, Helmholtzstrasse 20, D 89081 Ulm, Germany
    Blood 107:3342-9. 2006
    We have previously demonstrated that plasmin acts as a potent proinflammatory activator of human peripheral monocytes...
  47. ncbi Posterior vitreous detachment with plasmin in the isolated human eye
    Xiaoxin Li
    Department of Ophthalmology, People s Hospital, Peking University, Beijing, PRC
    Graefes Arch Clin Exp Ophthalmol 240:56-62. 2002
    To investigate the efficacy and safety of intravitreal injection of plasmin in producing posterior vitreous detachment (PVD), and the possible role of plasmin in degrading adhesion glycoproteins of the internal limiting membrane (ILM).
  48. ncbi Use of autologous plasmin during vitrectomy for diabetic maculopathy
    T Sakuma
    Department of Ophthalmology, Juntendo University, Urayasu Hospital, Urayasu, Chiba, Japan
    Eur J Ophthalmol 16:138-40. 2006
    To evaluate the efficacy of autologous plasmin enzyme as an adjunct to vitrectomy in diabetic macular edema.
  49. ncbi Plasmin-induced migration of endothelial cells. A potential target for the anti-angiogenic action of angiostatin
    Takehiko Tarui
    Department of Cell Biology, VB 6, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    J Biol Chem 277:33564-70. 2002
    ..b>Plasmin, the parent molecule of angiostatin and a major extracellular protease, induces platelet aggregation, migration ..
  50. ncbi Design, synthesis, and biological evaluation of a dual tumor-specific motive containing integrin-targeted plasmin-cleavable doxorubicin prodrug
    Franciscus M H de Groot
    Department of Organic Chemistry, NSR Center for Molecular Structure, Design and Synthesis, University of Nijmegen, 6525 ED Nijmegen, The Netherlands
    Mol Cancer Ther 1:901-11. 2002
    ..sequence is a D-Ala-Phe-Lys tripeptide that is selectively recognized by the tumor-associated protease plasmin, which is involved in tumor invasion and metastasis...
  51. ncbi Neuritogenesis and the nerve growth factor-induced differentiation of PC-12 cells requires annexin II-mediated plasmin generation
    A T Jacovina
    Division of Hematology-Oncology, Department of Pediatrics, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Biol Chem 276:49350-8. 2001
    ..A growing body of evidence suggests that this process may be dependent upon plasmin, a serine protease generated from plasminogen (Plg) by either urokinase Plg activator (u-PA) or tissue Plg ..
  52. ncbi Plasminogen/plasmin regulates alpha-enolase expression through the MEK/ERK pathway
    Lirlandia P Sousa
    Grupo de Transdução de Sinal, Departamento de Microbiologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, 31270 901 Belo Horizonte, Minas Gerais, Brazil
    Biochem Biophys Res Commun 337:1065-71. 2005
    Previously we have demonstrated that both plasminogen (Plg) and plasmin (Pla) regulate the expression of the transcription factors c-FOS and EGR-1 [L.P. De Sousa, B.S. Brasil, B.M. Silva, M.H. Freitas, S.V. Nogueira, P.C. Ferreira, E.G...
  53. ncbi Regulation of plasminogen binding to neutrophils
    T Herren
    Joseph J. Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, The Cleveland Clinic Foundation, Cleveland, OH, USA
    Blood 97:1070-8. 2001
    ..In support of this hypothesis, treatment of neutrophils with elastase, cathepsin G, or plasmin increased their plasminogen binding, and specific inhibitors of elastase and cathepsin G suppressed the up-..
  54. ncbi Plasmin enzyme-assisted vitreoretinal surgery in congenital X-linked retinoschisis: surgical techniques based on a new classification system
    Wei Chi Wu
    Associated Retinal Consultants, PC, Royal Oak, Michigan 48073, USA
    Retina 27:1079-85. 2007
    To review the surgical outcome of autologous plasmin enzyme-assisted vitreoretinal surgery in managing complications associated with congenital X-linked retinoschisis (CXLRS).
  55. ncbi Textilinin-1, an alternative anti-bleeding agent to aprotinin: Importance of plasmin inhibition in controlling blood loss
    Simone M Flight
    School of Medicine, University of Queensland, Brisbane, Australia
    Br J Haematol 145:207-11. 2009
    ..inhibitor from Pseudonaja textilis venom with sequence relatedness to aprotinin, is a potent but reversible plasmin inhibitor and has a narrower range of protease inhibition compared to aprotinin...
  56. ncbi Plasminogen-induced aggregation of PANC-1 cells requires conversion to plasmin and is inhibited by endogenous plasminogen activator inhibitor-1
    Naamit Deshet
    Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel
    J Cell Physiol 216:632-9. 2008
    ..We studied the effect of plasminogen (PGN), an inactive precursor of the PAR-1-activating protease, plasmin (PN) on aggregation of pancreatic adenocarcinoma (PDAC) cells...
  57. ncbi Eotaxin promotes eosinophil transmigration via the activation of the plasminogen-plasmin system
    C Ferland
    , Centre de Recherche, , , Laval, Quebec, Canada
    J Leukoc Biol 69:772-8. 2001
    ..4%, whereas inhibition of the plasminogen-plasmin system decreased eotaxin's effect by < or = 44.4% (P = 0.0002)...
  58. ncbi Studies of mice lacking plasminogen activator gene function suggest that plasmin production prior to ovulation exceeds the amount needed for optimal ovulation efficiency
    A Ny
    Department of Medical Biochemistry and Biophysics, Umea University, Sweden
    Eur J Biochem 244:487-93. 1997
    ..we have studied the regulation of plasmin activity in the ovaries of 25-day-old wild-type mice and mice with deficient PA gene function during gonadotropin-..
  59. pmc Acquisition of host plasmin activity by the Swine pathogen Streptococcus suis serotype 2
    Marie Claude Jobin
    Groupe de Recherche en Ecologie Buccale, Faculte de Medecine Dentaire, Universite Laval, Quebec City, Quebec, Canada
    Infect Immun 72:606-10. 2004
    ..One of the receptors was identified as glyceraldehyde-3-phosphate dehydrogenase. S. suis-associated plasmin activity was capable of activating free plasminogen, which in turn could contribute to degradation of fibronectin...
  60. ncbi Exploitation of astrocytes by glioma cells to facilitate invasiveness: a mechanism involving matrix metalloproteinase-2 and the urokinase-type plasminogen activator-plasmin cascade
    Duc M Le
    Department of Oncology, University of Calgary, Calgary, Alberta, Canada
    J Neurosci 23:4034-43. 2003
    ..coculture was investigated and was found to involve the urokinase-type plasminogen activator (uPA)-plasmin cascade whereby uPA bound to uPA receptor (uPAR), leading to the conversion of plasminogen to plasmin...
  61. ncbi Activated thrombin-activatable fibrinolysis inhibitor reduces the ability of high molecular weight fibrin degradation products to protect plasmin from antiplasmin
    Mark Schneider
    Departments of Biochemistry and Medicine, Queen s University, Kingston Ontario K7L 3N6, Canada
    J Biol Chem 279:13340-5. 2004
    ..B-like plasma enzyme that can slow clot lysis by removing lysine residues exposed on fibrin as it is cleaved by plasmin. Previously, it was shown that fibrin treated with TAFIa is less able to promote plasminogen activation by tissue-..
  62. ncbi A mediator of cell surface-specific plasmin generation
    C Brownstein
    Department of Pediatrics, Weill Medical College of Cornell University, New York, New York 10021, USA
    Ann N Y Acad Sci 947:143-55; discussion 155-6. 2001
    It has become increasingly evident that the generation of cell surface proteases including plasmin is fundamental to a wide variety of in vivo biological processes...
  63. ncbi Inhibitory role of eosinophils on cell surface plasmin generation by bronchial epithelial cells: inhibitory effects of transforming growth factor beta
    K Hara
    Department of Medicine II, Niigata University School of Medicine, Niigata, Japan
    Lung 179:9-20. 2001
    ..We studied the effect of eosinophils on cell surface plasmin generation by bronchial epithelial cells, because plasmin is thought to be involved in bronchial tissue repair/..
  64. pmc The role of seladin-1/DHCR24 in cholesterol biosynthesis, APP processing and Abeta generation in vivo
    Arames Crameri
    Division of Psychiatry Research, University of Zurich, Zurich, Switzerland
    EMBO J 25:432-43. 2006
    ..This was associated with inefficient plasminogen binding and plasmin activation, the displacement of beta-secretase (BACE) from DRMs to APP-containing membrane fractions, increased ..
  65. ncbi Nonclinical safety and pharmacokinetics of intravitreally administered human-derived plasmin in rabbits and minipigs
    Joel W Proksch
    Global Preclinical Development, Bausch and Lomb, Rochester, NY 14609, USA
    J Ocul Pharmacol Ther 24:320-32. 2008
    The use of plasmin for pharmacologic vitreolysis and the creation of a posterior vitreous detachment offers several potential advantages over surgery...
  66. ncbi Plasmin/plasminogen is essential for the healing of tympanic membrane perforations
    Jinan Li
    Department of Medical Biochemistry and Biophysics, Umea University, Umea, Sweden
    Thromb Haemost 96:512-9. 2006
    ..and re-epithelialization were completely arrested in plasminogen-deficient mice indicates that plasminogen/plasmin plays a more profound role in the healing of tympanic membrane perforations than in the healing of other ..
  67. ncbi Annexin A2 regulates the levels of plasmin, S100A10 and Fascin in L5178Y cells
    Yingchun Hou
    Department of Cell Biology, School of Life Sciences, Shaanxi Normal University, Xi an, Shaanxi, China
    Cancer Invest 26:809-15. 2008
    Annexin A2 (ANXA2) was reported as the receptor, activator, expression enhancer, or cooperator for plasmin, S100A10, and others...
  68. ncbi Effects of metal ions on activity of plasmin
    Pawel Nowak
    Department of General Biochemistry, University of Lodz, Lodz, Poland
    Biol Trace Elem Res 93:87-94. 2003
    The effects of some metal ions on amidolytic and fibrinogenolytic activities of highly purified human plasmin were investigated in vitro...
  69. pmc Receptor-independent role of urokinase-type plasminogen activator in pericellular plasmin and matrix metalloproteinase proteolysis during vascular wound healing in mice
    P Carmeliet
    The Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium
    J Cell Biol 140:233-45. 1998
    ..Pericellular plasmin proteolysis, evaluated by degradation of 125I-labeled fibrin and activation of zymogen matrix metalloproteinases, ..
  70. ncbi Reduction of myocardial infarct size by doxycycline: a role for plasmin inhibition
    Michael O Griffin
    Department of Medicine, University of California, San Diego, CA 2103 8412, USA
    Mol Cell Biochem 270:1-11. 2005
    ..We hypothesized that activation of MMPs and the serine protease plasmin contribute to early cardiac myocyte death following I/R and that broad-spectrum protease inhibition with ..
  71. ncbi p11 regulates extracellular plasmin production and invasiveness of HT1080 fibrosarcoma cells
    Kyu Sil Choi
    Cancer Biology Research Group, Department of Medical Science, University of Calgary Calgary, Alberta, Canada T2N4N1
    FASEB J 17:235-46. 2003
    ..Most cancer cells secrete the urokinase-type plasminogen activator, which converts cell-bound plasminogen to plasmin. Here we address the issue of whether the plasminogen binding protein, p11, plays a significant role in this ..
  72. ncbi Cellular mechanisms regulating non-haemostatic plasmin generation
    R Bass
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U K
    Biochem Soc Trans 30:189-94. 2002
    ..A central mechanism for regulating plasmin generation is through the binding of the two plasminogen activators to specific cellular receptors: urokinase-..
  73. ncbi Thrombolytic potency of acid-stabilized plasmin: superiority over tissue-type plasminogen activator in an in vitro model of catheter-assisted thrombolysis
    V Novokhatny
    Bayer Corporation, Biological Products Division, Research Triangle Park, North Carolina 27709, USA
    J Thromb Haemost 1:1034-41. 2003
    b>Plasmin, the direct fibrinolytic enzyme, was compared with tissue plasminogen activator (t-PA) in an in vitro thrombolysis model...
  74. pmc Plasminogen activation/plasmin in rheumatoid arthritis: matrix degradation and more
    Martin O Judex
    Sidney Kimmel Cancer Center, San Diego, California, USA
    Am J Pathol 166:645-7. 2005
    ..1,2) Through the use of an experimental mouse model of RA, Li and colleagues(6) have implicated plasmin as an essential component of the early phase of RA, as reported in this issue of The American Journal of Pathology...
  75. ncbi High-molecular-mass and low-molecular-mass kininogens block plasmin-induced platelet aggregation by forming a complex with kringle 5 of plasminogen/plasmin
    T E Selim
    Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Eur J Biochem 250:532-8. 1997
    ..67 microM) or LK (3 microM) with Plg (1.5 microM) did not affect its conversion to plasmin by tissue plasminogen activator (t-PA) (10 U/ml) in the presence of a tissue plasminogen stimulator (0.14 microM)...
  76. ncbi Identification of amino acids in antiplasmin involved in its noncovalent 'lysine-binding-site'-dependent interaction with plasmin
    Haiyao Wang
    Department of Clinical Chemistry and Blood Coagulation, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden
    Eur J Biochem 270:2023-9. 2003
    The lysine-binding-site-mediated interaction between plasmin and antiplasmin is of great importance for the fast rate of this reaction. It also plays an important part in regulating the fibrinolytic enzyme system...
  77. pmc Role of the streptokinase alpha-domain in the interactions of streptokinase with plasminogen and plasmin
    Ronald R Bean
    Department of Pathology, School of Medicine, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 280:7504-10. 2005
    The role of the streptokinase (SK) alpha-domain in plasminogen (Pg) and plasmin (Pm) interactions was investigated in quantitative binding studies employing active site fluorescein-labeled [Glu]Pg, [Lys]Pg, and [Lys]Pm, and the SK ..
  78. ncbi Laminin chain expression suggests that laminin-10 is a major isoform in the mouse hippocampus and is degraded by the tissue plasminogen activator/plasmin protease cascade during excitotoxic injury
    J A Indyk
    Department of Pharmacology, University at Stony Brook, Stony Brook, NY 11794 8651, USA
    Neuroscience 116:359-71. 2003
    ..The tissue plasminogen activator/plasmin extracellular protease cascade and downstream laminin degradation are implicated in excitotoxin-induced neuronal ..
  79. ncbi Protective effects of plasmin(ogen) in a mouse model of Staphylococcus aureus-induced arthritis
    Yongzhi Guo
    Umea University, Umea, Sweden
    Arthritis Rheum 58:764-72. 2008
    To assess the functional roles of plasmin in a murine model of Staphylococcus aureus-induced bacterial arthritis.
  80. ncbi Plasmin-mediated processing of protein tyrosine phosphatase receptor type Z in the mouse brain
    Jeremy Pak Hong Chow
    Division of Molecular Neurobiology, National Institute for Basic Biology, 5 1 Higashiyama, Myodaiji cho, Okazaki, Aichi 444 8787, Japan
    Neurosci Lett 442:208-12. 2008
    ..Here, we identified the proteolytic processing of Ptprz by plasmin in the mouse brain, which is markedly enhanced after kainic acid (KA)-induced seizures...
  81. ncbi A comparison of cyclohexanone and tetrahydro-4H-thiopyran-4-one 1,1-dioxide as pharmacophores for the design of peptide-based inhibitors of the serine protease plasmin
    Fengtian Xue
    Department of Chemistry, Brown University, Providence, Rhode Island 02912, USA
    J Org Chem 70:8309-21. 2005
    ..The serine protease plasmin has a central role in the plasminogen system...
  82. doi Evoking plasmin for beta-amyloid clearance
    Jian Zhao
    Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Cell Res 18:803-4. 2008
  83. ncbi On the mechanism of the reaction between human alpha 2-antiplasmin and plasmin
    B Wiman
    J Biol Chem 254:9291-7. 1979
  84. ncbi Plasmin cleaves the juxtamembrane domain and releases truncated species of the urokinase receptor (CD87) from human bronchial epithelial cells
    Nathalie Beaufort
    Unité de Défense Innée et Inflammation Inserm E336, Département de Médecine Moléculaire, Institut Pasteur, 25 28 Rue du Docteur Roux, F 75724 Paris Cedex 15, France
    FEBS Lett 574:89-94. 2004
    ..soluble CD87 and to CD87-expressing epithelial cells, we establish that exogenous or in situ generated plasmin proteolyzes CD87 in the D1-D2 linker and D3 carboxyterminal sequences, producing a major soluble D2D3 species...
  85. pmc The serine protease plasmin cleaves the amino-terminal domain of the NR2A subunit to relieve zinc inhibition of the N-methyl-D-aspartate receptors
    Hongjie Yuan
    Department of Pharmacology, Center for Neurodegenerative Disease, Emory University School of Medicine, Atlanta, GA 30322 3090, USA
    J Biol Chem 284:12862-73. 2009
    ..The serine protease plasmin can cleave a number of substrates, including protease-activated receptors, and may play an important role in ..
  86. ncbi [Enzymatic vitrectomy by intravitreal autologous plasmin injection, as initial treatment for diffuse diabetic macular edema]
    M Diaz-Llopis
    Departamento de Oftalmologia, Hospital Geneneral Universitario, Unidad Docente de Oftalmología, Facultad de Medicina, Universidad de Valencia, Valencia, Espana
    Arch Soc Esp Oftalmol 83:77-84. 2008
    To determine whether intravitreal injection of plasmin is effective in treating diffuse diabetic macular edema (DDME).
  87. pmc Association studies between the plasmin genes and late-onset Alzheimer's disease
    Nobuto Shibata
    Centre for Research in Neurodegenerative Diseases, Department of Medicine, University of Toronto and Toronto Western Hospital Research Institute, Toronto, Ontario, Canada
    Neurobiol Aging 28:1041-3. 2007
    The plasmin system is involved in the degradation of Abeta peptides, the accumulation of which in brain is a hallmark of Alzheimer's disease (AD)...
  88. ncbi A proteinase inhibitor from Caesalpinia echinata (pau-brasil) seeds for plasma kallikrein, plasmin and factor XIIa
    Ilana Cruz-Silva
    Departamento de Bioquimica, Universidade Federal de Sao Paulo, 04044 020 Sao Paulo, SP, Brazil
    Biol Chem 385:1083-6. 2004
    ..CeKI inhibits human plasma kallikrein ( K i =3.1 nM), plasmin ( K i =0.18 nM), factor XIIa ( K i =0.18 nM), trypsin ( K i =21.5 nM) and factor Xa ( K i =0.49 mM)...
  89. ncbi Simplified recombinant plasmin: production and functional comparison of a novel thrombolytic molecule with plasma-derived plasmin
    Jennifer A Hunt
    Talecris Biotherapeutics, 85 TW Alexander Dr, Research Triangle Park, NC 27709, USA
    Thromb Haemost 100:413-9. 2008
    ..mutant Delta(K2-K5)Pm by plasminogen activators was evaluated, and functional characteristics of the simplified plasmin were studied. Properties of Delta(K2-K5)Pg were similar to native, human plasma-derived plasminogen...
  90. ncbi Plasmin-dependent and -independent effects of plasminogen activators and inhibitor-1 on ex vivo angiogenesis
    S Brodsky
    Department of Medicine, State University of New York, Stony Brook, New York 11794 8152, USA
    Am J Physiol Heart Circ Physiol 281:H1784-92. 2001
    ..gain insight into the requirement for different matrix-degrading systems during endothelial cell migration across plasmin-degradable basement membranes compared with profibrotic areas containing plasmin-nondegradable collagen, we ..
  91. ncbi Effect of external ocular surgery and mode of post-operative care on plasminogen, plasmin, angiostatins and alpha(2)-macroglobulin in tears
    M Lembach
    SUNY College of Optometry, 33 West 42nd Street, New York, NY 10036, USA
    Curr Eye Res 22:286-94. 2001
    ..whether corneal surgery and the mode of post-surgical treatment influence the distribution of plasminogen, plasmin, angiostatins and alpha(2)-macrogobulin in tear fluid. METHODS...
  92. ncbi The local chromaffin cell plasminogen/plasmin system and the regulation of catecholamine secretion
    Qijiao Jiang
    Department of Medicine, University of California, and San Diego VA Healthcare System, San Diego, California 92161, USA
    Ann N Y Acad Sci 971:445-9. 2002
    Chromaffin cells express components of the plasminogen/plasmin system, including its major activator, tissue plasminogen activator (t-PA), and high-affinity cellular receptors for plasminogen, which promote local concentration and ..
  93. ncbi Plasmin in renal interstitial fibrosis: innocent or guilty?
    Guoping Zheng
    Kidney Int 66:455-6. 2004
  94. ncbi Cloning, sequencing and functional overexpression of the Streptococcus equisimilis H46A gapC gene encoding a glyceraldehyde-3-phosphate dehydrogenase that also functions as a plasmin(ogen)-binding protein. Purification and biochemical characterization of
    K Gase
    Institute for Molecular Biology, Jena University, Germany
    Eur J Biochem 239:42-51. 1996
    ..prerequisite to understanding possible mechanisms that control the balance between the plasminogen activating and plasmin(ogen) binding capacities of H46A, we describe here its gapC gene encoding glyceraldehyde-3-phosphate ..
  95. ncbi Plasmin-induced expression of cytokines and tissue factor in human monocytes involves AP-1 and IKKbeta-mediated NF-kappaB activation
    T Syrovets
    Department of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, Helmholtzstrasse 20, D-89081 Ulm, Germany
    Blood 97:3941-50. 2001
    It was previously shown that plasmin activates human peripheral monocytes in terms of lipid mediator release and chemotactic migration...
  96. ncbi Regulation of plasmin-induced protease-activated receptor 4 activation in platelets
    Yingying Mao
    Department of Physiology, Temple University School of Medicine, Philadelphia, PA, USA
    Platelets 20:191-8. 2009
    b>Plasmin, a major extracellular protease, activates platelets through PAR4 receptors. Plasmin-induced full aggregation is achieved at lower concentrations (0.1 U/mL) in murine platelets as compared to human platelets (1 U/mL)...
  97. ncbi Pediatric traumatic macular hole: results of autologous plasmin enzyme-assisted vitrectomy
    Wei Chi Wu
    Associated Retinal Consultants, PC, Royal Oak, Michigan 48073, USA
    Am J Ophthalmol 144:668-672. 2007
    To review our experience with plasmin-assisted vitrectomy surgery for the treatment of pediatric macular holes.
  98. ncbi Urokinase-type plasminogen activator stimulation of monocyte matrix metalloproteinase-1 production is mediated by plasmin-dependent signaling through annexin A2 and inhibited by inactive plasmin
    Yahong Zhang
    Immunopathology Section, National Institute of Dental and Craniofacial Research NIH, Bethesda, MD 20892, USA
    J Immunol 179:3297-304. 2007
    ..Urokinase-type plasminogen activator (uPA) and plasmin, in addition to their role in fibrinolysis and activation of pro-MMPs, have been shown to transduce intracellular ..
  99. ncbi Pericellular plasmin induces smooth muscle cell anoikis
    Olivier Meilhac
    INSERM U460, CHU Bichat Claude Bernard, 75018 Paris, France
    FASEB J 17:1301-3. 2003
    ..Using primary cultures of arterial SMCs, we show that plasmin generated from plasminogen on the cell surface induces cell retraction and fibronectin fragmentation, leading to ..
  100. ncbi Role of tissue plasminogen activator/plasmin cascade in delayed neuronal death after transient forebrain ischemia
    Hiroshi Takahashi
    Department of Neurosurgery, Fujinomiya City General Hospital, Fujinomiya shi, Shizuoka ken 418 0076, Japan
    Neurosci Lett 381:189-93. 2005
    We studied the possible involvement of the tissue plasminogen activator (t-PA)/plasmin system on both delayed neuronal death in the hippocampus and the associated enhancement of locomotor activity in rats, after transient forebrain ..
  101. ncbi A long-lasting, plasmin-activatable thrombin inhibitor aids clot lysis in vitro and does not promote bleeding in vivo
    William P Sheffield
    McMaster University, Department of Pathology and Molecular Medicine, HSC 4N66, 1200 Main Street West, Hamilton, Ontario, Canada, L8N 3Z5
    Thromb Haemost 101:867-77. 2009
    ..reconfiguration of the chimera, appending human serum albumin (HSA) to the N-terminus of HV3, with an intervening plasmin cleavage site, would create a slowly cleared, plasmin-activatable HV3...

Research Grants62

  1. Serpin Modulation of Inflammatory Vasculitis; Potential for Immunomodulatory Ther
    ALEXANDRA ROSE LUCAS; Fiscal Year: 2010
    ..These serpins target protease cascades in the clot dissolving thrombolytic (uPA, tPA for Serp-1 and NSP;plasmin for Serp-1 ) and clot forming thrombotic pathways (fXa for Serp-1)...
  2. Cross-talk Between Coagulation, Inflammation, and Immunity
    Lawrence L Leung; Fiscal Year: 2013
    ..plays a broad anti-inflammatory role in vivo;the targets for CPB include bradykinin, C5a, the generation of plasmin and thrombin-cleaved osteopontin (OPN-Arg or OPN-R)...
  3. Clinical Development of Hemostatic Agent ClotFoam for Severe Hemorrhage
    Maja Nowakowski; Fiscal Year: 2013
    ..ClotFoam(R) is also a plasmin-depleted aprotinin-and-thrombin-free fibrin sealant presenting no risk of autoimmune reaction to blood products...
  4. Dual Direct Inhibitors of Thrombin and Factor Xa
    UMESH RAMANLAL DESAI; Fiscal Year: 2012
    ..with proteins (factors IIa, Xa, XIa, XIIa, XIIIa, IXa, VIIa, antithrombin, kallikrein, activated protein C plasmin, trypsin, etc...
  5. The Role of Carboxypeptidase B and its Substrates in Rheumatoid Arthritis
    William H Robinson; Fiscal Year: 2013
    ..an anti-fibrinolytic role by removing C-terminal lysine residues from fibrin, thereby preventing its cleavage by plasmin. Recently, C3a, C5a, thrombin-cleaved osteopontin (OPN-R) and bradykinin were identified as additional substrates ..
  6. Regulation of Leukocyte Migration by Plasminogen
    JANE LEE HOOVER-PLOW; Fiscal Year: 2013
    ..mice in peritonitis and abdominal aortic aneurysm (AAA) models, our studies provide in vivo evidence that Plg/plasmin is required for macrophage recruitment and this role is mediated through activation of MMP-9...
  7. Cell Surface Plasminogen Activation and Hypertension
    Robert J Parmer; Fiscal Year: 2013
    ..role for serine protease-mediated cleavage of ENaC subunits in ENaC activation, and recent studies suggest that plasmin is a key protease in this process...
  8. Cthrc1 function as a novel TGF-beta antagonist in the vasculature
    Volkhard Lindner; Fiscal Year: 2013
    ..the TGF-ss signaling pathway, and iii) to determine the function of the identified Cthrc1 fragments generated by plasmin. This proposal will employ mouse models of Cthrc1 gain-of-function and loss-of function, in combination with ..
  9. PLASMINOGEN AND PLASMIN: STRUCTURE AND FUNCTION
    Francis J Castellino; Fiscal Year: 2013
    ..Through this overal mechanism, M and M-like proteins enhance the activation of Pg to the serine protease, plasmin (Pm), by bacterial or host Pg activators, thus creating a proteolytic bacterial microenvironment employed by GAS ..
  10. Plasminogen activator inhibitor-1 in tumor progression and metastasis
    Yves A DeClerck; Fiscal Year: 2013
    ..of the family of endogenous serine protease inhibitors (serpins) that controls the activation of plasminogen into plasmin by tissue and urokinase type plasminogen activators (tPA and uPA, respectively)...
  11. MATRIX MEDIATORS OF WOUND HEALING
    Paul J Higgins; Fiscal Year: 2010
    Plasminogen activator inhibitor type-1 (PAI-1) is the major physiologic regulator of the plasmin-based pericellular proteolytic cascade and a critical element in pathologic angiogenesis in vivo. PAI-1 controls stromal proteolysis (i.e...
  12. Neonatal Anesthetic Neurotoxicity
    Piyush M Patel; Fiscal Year: 2013
    ..from synaptic vesicles as a pro molecule (proBDNF) and undergoes proteolytic cleavage in the synaptic cleft by plasmin to generate mature BDNF (mBDNF) [19]...
  13. Mini-plasmin: Pre-Clinical Evaluation of a Novel Thrombolytic Strategy for Stroke
    Irina Y Sazonova; Fiscal Year: 2012
    ..for development of new therapeutic agents for stroke, submits this Innovative Research Proposal entitled "Mini-plasmin: Pre-Clinical Evaluation of a Novel Thrombolytic Strategy for Stroke"...
  14. The role of the terminal complement pathway in experimental cerebral malaria.
    Theresa N Ramos; Fiscal Year: 2012
    ..What is the relative importance of extrinsic protease activation pathway (via proteases such as thrombin and plasmin) to C5- mediated inflammation in ECM? Based on our preliminary observations, we hypothesize that C5 is the ..
  15. Modulation of the Epithelial Sodium Channel by Proteases and Peptide Inhibitors
    CHRISTOPHER JOHN PASSERO; Fiscal Year: 2010
    ..Other serine proteases besides furin and prostasin can activate these channels. Recent evidence suggests plasmin and matrix metalloproteinases activate ENaC...
  16. Mesenchymal Cell Fate Determination by Extracellular Matrix Stiffness
    Jeffrey C Horowitz; Fiscal Year: 2013
    ..Moreover, plasmin-mediated ECM proteolysis, which induces myofibroblast apoptosis, is associated with the loss of FAK activity...
  17. Role of the Plasminogen Activator Protease during Pneumonic Plague
    Wyndham W Lathem; Fiscal Year: 2013
    ..In vitro, Pla converts host plasminogen to the active plasmin form...
  18. Novel Methods for Dissolving Blood Clots
    GUY LELAND REED; Fiscal Year: 2013
    ..discovered a molecule that dissolves blood clots through a unique mechanism-inactivating the major inhibitor of plasmin. Through synergism, this molecule increases the potency and specificity of TPA, and it avoids TPA-related ..
  19. PAR-1 Signaling and HAND
    KATHLEEN ANNE MAGUIRE-ZEISS; Fiscal Year: 2013
    ..Levels of PAR-1 activators, including plasmin and matrix metalloproteinases (MMPs), are substantially increased in HIV associated neurological disorders (HAND) ..
  20. Preclinical Development of a Novel Plaque-Regressing Therapy For Atherosclerosis
    Michael W Fanger; Fiscal Year: 2012
    ..model of atherosclerosis, that rPAI-123 stimulates plaque regression in a dose-dependent manner through the novel plasmin regulatory pathway, thereby identifying the optimal rPAI-123 dosing for future pre-clinical studies;and 2) to ..
  21. Hyperamylinemia in Diabetic Heart Disease: Mechanisms, Responses, and Prevention
    Florin Despa; Fiscal Year: 2013
    ..The pilot study also suggests that endogenous molecules with anti-aggregation properties, such as plasmin and epoxyeicosanoids, limit cardiac accumulation of amylin and its myopathic response...
  22. MOLECULAR MECHANISMS OF FIBRINOLYSIS
    Paul E Bock; Fiscal Year: 2013
    ..streptococcal virulence factor, targets human fibrinolysis by activating plasminogen (Pg) to the proteinase, plasmin (Pm)...
  23. Multiscale, Multiphysics Model of Thrombus Biomechanics in Aortic Dissection
    George Karniadakis; Fiscal Year: 2013
    ..within the false lumen and that partially thrombosed dissections are dangerous because the continued release of plasmin by an intramural thrombus in contact with flowing blood can activate constitutively produced, latent matrix ..
  24. Continuous monitoring of anti-fibrinolytic therapy in cardiovascular surgery
    Francis G Spinale; Fiscal Year: 2012
    ..which is commonly managed by pharmacologically modifying clot degradation (fibrinolysis), through inhibition of plasmin activity (PLact)...
  25. Deep Vein Thrombosis and Chronic Thrombotic Venous Disease
    Timothy A Morris; Fiscal Year: 2012
    ..studies disclosed that fibrinogen purified from CTEPH patients is somewhat resistant to lysis by exogenous plasmin. Furthermore, we have identified specific fibrinogen mutations among CTEPH patients that may affect the structure ..
  26. Role of Protease Activated Receptors in Spinal Cord Injury and Repair
    Isobel A Scarisbrick; Fiscal Year: 2013
    ..to establish the role of PARs in mediating the cellular effects of SCI-related PAR agonists (KLKs, thrombin and plasmin) in primary spinal cord neurons, astrocytes and oligodendroglia and their sensitivity to neurotoxic agents in ..
  27. Efficacy of Kunitz Domain 1 variants of TFPI-2 as novel antifibrinolytic agents
    MADHU SATYA BAJAJ; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): The goal of this proposal remains to test a novel and unique inhibitor of plasmin that has the potential to reduce severe bleeding in major surgery as well as in coagulopathy...
  28. Regulation of plasminogen-dependent cell functions by the novel receptor, Plg-RKT
    Lindsey A Miles; Fiscal Year: 2013
    ..Localization of plasminogen on cell surfaces is a crucial control point for positive regulation of cell surface plasmin proteolytic activity that facilitates both physiological and pathological processes requiring cell migration...
  29. Recombinant annexin A2 plus tPA for combination stroke therapy
    Xiaoying Wang; Fiscal Year: 2013
    ..The molecular basis for our idea is that annexin A2 accelerates the generation of clot-dissolving plasmin by assembling a fibrinolytic complex with tPA and the plasmin precursor plasminogen. Our pilot data are promising...
  30. Annexin 2 in Lymphoproliferative Angiogenesis
    Jia Ruan; Fiscal Year: 2012
    ..neo-angiogenesis by mediating vascular and stromal cell recruitment, migration, and assembly via cell surface plasmin generation and matrix metalloproteinases activation...
  31. Understanding the biological activity of Cthrc1 cleavage fragments
    RENEE LECLAIR; Fiscal Year: 2010
    ..b>Plasmin is capable of cleaving Cthrd in two locations and the activity of these fragments will be assessed in vitro using ..
  32. A Novel Plasminogen Receptor in Breast Cancer
    BARBARA MARIA MUELLER; Fiscal Year: 2013
    ..the plasminogen receptor, Plg-RKT, which promotes activation of plasminogen to the broad spectrum protease, plasmin. Cell surface plasmin regulates the tumor microenvironment by effecting degradation of the ECM and release of ..
  33. Plasminogen Activation &SK: Structure-Function
    Guy L Reed; Fiscal Year: 2012
    ..7 million people a year worldwide. Therapeutic dissolution of these thrombi by plasmin, an enzyme that digests fibrin, reduces death and disability from heart attacks and strokes...
  34. RESOLUTION OF GLOMERULOSCLEROSIS
    Agnes B Fogo; Fiscal Year: 2013
    ..This regression was linked to decreased matrix and restoration of plasmin. Our new preliminary data show limited long-term efficacy over six months of this intervention, indicating ..
  35. Control of fibroblast function by prostaglandin E2 and plasminogen activation
    MARC L PETERS-GOLDEN; Fiscal Year: 2013
    ..and cyclic AMP (cAMP) effectors, and 2) the proteolytic cascade by which urokinase converts plasminogen to plasmin. We have previously shown that cross-talk between these two pathways is critical for their anti-fibrotic ..
  36. Designing Fibrin Microthread Scaffolds for Skeletal Muscle Regeneration
    Jonathan M Grasman; Fiscal Year: 2013
    ..In the wound site, fibrin degrades quickly i the presence of numerous proteinases such as plasmin. We recently developed a crosslinking strategy to modify fibrin microthreads to increase their resistance to ..
  37. Regulation of urokinase receptor expression in colon cancer
    Douglas D Boyd; Fiscal Year: 2010
    ..The u- PAR also binds the serine protease urokinase thus increasing plasmin formation and extracellular matrix protein turnover to promote tumor cell migration/invasion...
  38. Nuclear urokinase (uPA) regulates tumor cell proliferation and survival.
    Victoria Stepanova; Fiscal Year: 2010
    ..factor-like domain (GFD), which binds uPAR, the kringle and a protease domain which converts plasminogen into plasmin and cleaves some other substrates...
  39. Workshop on the Molecular and Cellular Biology of Plasminogen Activation
    Victoria Ploplis; Fiscal Year: 2013
    ..ultimately results in the proteolytic conversion of the plasma zymogen, plasminogen (Pg), to the serine protease plasmin (Pm) (1)...
  40. MSCs Induce Brain Plasticity via tPA
    Michael Chopp; Fiscal Year: 2013
    ..Hypothesis 3: tPA activity increased by MSCs promotes neurite remodeling via plasmin-dependent proteolytic cleavage of pro-neurotrophins: pro-nerve growth factor (pro-NGF) to NGF, pro-brain derived ..
  41. Corneal Repair: uPA and extracellular matrix
    Audrey M Bernstein; Fiscal Year: 2010
    ..When uPA binds to its cell-surface receptor, uPAR, uPA generates plasmin at the cell-matrix interface...
  42. uPA/uPAR and Ro60 in the pathogenesis of congenital heart block
    PARASKEVI BRIASOULI; Fiscal Year: 2013
    ..eat me" signal to healthy cardiocytes attempting clearance of remodeling cells and the other is the generation of plasmin. Aim 1 explores the mechanisms by which anti-Ro60 exploits uPAR to inhibit apoptotic clearance. In Aim 2, Dr...
  43. PA/PLASMIN/GELATINASE CASCADE IN DIABETIC NEPHROPATHY
    WILLIAM BARICOS; Fiscal Year: 2000
    ..that extracellular matrix degradation by cultured mesangial cells involves cooperative interactions between plasmin (generated from plasminogen by MC plasminogen activator) and mesangial cell gelatinase...
  44. PROTEINASE IN FIBRINOLYSIS AND THROMBOLYTIC THERAPY
    STEVEN GONIAS; Fiscal Year: 1993
    ..The first aim is to understand the reaction of the thrombolytic complex, streptokinase-plasmin(ogen)(SkPl), preparations and on the importance of cofactor such as fibrinogen, fibrin digestion products and ..
  45. Role of Inflammatory Neutrophils and their Unique MMP-9 in Tumor Progression
    James P Quigley; Fiscal Year: 2013
    ..The plasminogen/plasmin serine protease cascade will be examined mechanistically for partnering with activated MMP-9 during angiogenic ..
  46. HEMOSTATIC EFFECTS OF FIBRINOLYTIC THERAPY
    BARBARA ALEVRIADOU; Fiscal Year: 2000
    ..Bach agent results in cleavage of plasminogen to plasmin. Plasmin degrades the fibrin strands and dissolves thrombi...
  47. Reducing Stroke by a Novel, Clot-dissolving Antibody
    GUY LELAND REED; Fiscal Year: 2013
    ..studies, we have shown that these goals might be achieved by a molecule that inactivates the major inhibitor of plasmin and, dissolves clots through a unique mechanism that avoids the risk of hemorrhage and neurotoxicity associated ..
  48. INTERACTION OF GROUP A STREP WITH THE PLASMINOGEN SYSTEM
    Michael Boyle; Fiscal Year: 2001
    ..Studies in Dr. Boyle's laboratory have shown that group A streptococci can interact with the human plasmin(ogen) system by a number of different mechanisms to acquire surface proteolytic enzymatic activity, which cannot ..
  49. tPA Neurotoxicity in Hypoxic-Ischemia Encephalopathy
    Chia Yi Kuan; Fiscal Year: 2009
    ..Anti-plasmin treatment reduces the extent of ischemic- hypoxic brain injury, whereas the injection of exogenous tPA worsens it...
  50. PDGF and PVR
    Andrius Kazlauskas; Fiscal Year: 2012
    ..was the predominant PDGF isoform in the vitreous of patients and experimental animals experiencing PVR, and that plasmin was the major protease that processed it to the CUB and core domains...
  51. ENVIRONMENTAL MODULATION OF FIBRINOLYSIS INHIBITORS
    STEVEN GONIAS; Fiscal Year: 1992
    ..b>Plasmin is the primary proteinase responsible for fibrin digestion in vivo...
  52. STRUCTURE/FUNCTION STUDIES ON PLASMINOGEN AND PLASMIN
    Francis Castellino; Fiscal Year: 1999
    ..Important new thrombolytic agents, and/or strategies for application of thrombolytic therapy to human vascular disease, is expected to directly or indirectly result from the basic knowledge provided by this work. ..
  53. Mechanisms of mosquito midgut invasion by Plasmodium ookinetes
    Marcelo Jacobs-Lorena; Fiscal Year: 2013
    ..facilitates both ookinete docking to the surface of the midgut epithelium and tPA activation of plasminogen into plasmin (a protease)...
  54. REGULATION OF ANGIOGENESIS BY PAI1
    Daniel Lawrence; Fiscal Year: 2001
    ..plasminogen activator (tPA) both activate the broad specificity zymogen plasminogen into its active form plasmin, and both uPA and plasmin have previously been implicated in tissue remodeling in vivo, including wound healing ..
  55. tPA is a Neuroprotectant in the Ischemic Brain
    MANUEL SALVADOR YEPES; Fiscal Year: 2013
    ..Tissue-type plasminogen activator (tPA) is a serine proteinase that activates the zymogen plasminogen into plasmin. In the intravascular space tPA has a beneficial thrombolytic effect...
  56. Recombinant Des-Kringle Derivatives of Plasmin for Treatment of Peripheral Arteri
    XLNLI LIN; Fiscal Year: 2006
    ..Additionally, problems with long, retracted clots and poor circulation results in depletion of available plasmin, the PA substrate, near the PAO thrombus, rendering the PA only partially effective...
  57. Alpha2-antiplasmin polymorphism and a new plasma enzyme
    Patrick McKee; Fiscal Year: 2006
    ..proposal's goal is to (1) define interactions that determine how much a2AP becomes clot-bound for inhibiting plasmin; and (2) identify means of regulating a2AP so that plasmin activity might be enhanced long-term in diseases where ..
  58. A New Class of Serine and Cysteine Protease Inhibitors
    CHRISTOPHER SETO; Fiscal Year: 2006
    ..The serine protease plasmin plays a role in both angiogenesis and metastasis in cancer development, while PTP1B and the Yersinia PTPase are ..
  59. Site Directed Thrombolytic Agent
    ELEANOR MCGOWAN; Fiscal Year: 2003
    ..Active plasmin is generated from a stable depot form of plasmin, AZ-plasmin, by exposure to energy (light, sound waves)...
  60. Intrapulmonary Gene Transfer Using Compacted DNA
    Mark Cooper; Fiscal Year: 2001
    ..Copernicus Therapeutics has evaluated intratracheal delivery of proprietary formulations of compacted DNA (PLASmin complexes)...
  61. ANNEXIN II & PLASMIN MEDIATED HIGH GRADE GLIOMA INVASION
    Suchitra Acharya; Fiscal Year: 2002
    ..The plasminogen-plasmin system directly or, by activating matrix metalloproteinases (MMPs) has been implicated in the proteolytic ..
  62. ANTICARDIOLIPIN ANTIBODIES IN ANTIPHOSPHOLIPID SYNDROME
    Pojen Chen; Fiscal Year: 2007
    ..Recently, we found 4/5 patient-derived, prothrombotic, monoclonal IgG aCL bind to plasmin, thrombin and activated protein C (APC)...