chymotrypsin

Summary

Summary: A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.

Top Publications

  1. ncbi Sheep mast cell proteinase-1: characterization as a member of a new class of dual-specific ruminant chymases
    A D Pemberton
    Department of Veterinary Clinical Studies, Royal Dick School of Veterinary Studies, University of Edinburgh, Roslin, Scotland, U K
    Biochem J 321:665-70. 1997
  2. ncbi Isolation and primary structures of seven serine proteinase inhibitors from Cyclanthera pedata seeds
    Jolanta Kowalska
    Institute of Biochemistry and Molecular Biology, University of Wrocław, Tamka 2, 50 137 Wrocław, Poland
    Biochim Biophys Acta 1760:1054-63. 2006
  3. ncbi Picornaviral 3C cysteine proteinases have a fold similar to chymotrypsin-like serine proteinases
    M Allaire
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Nature 369:72-6. 1994
  4. ncbi A novel erythrocyte binding antigen-175 paralogue from Plasmodium falciparum defines a new trypsin-resistant receptor on human erythrocytes
    Tim-Wolf Gilberger
    Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
    J Biol Chem 278:14480-6. 2003
  5. ncbi Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra alpha-helical domain
    Kanchan Anand
    Department of Structural Biology and Crystallography, Institute of Molecular Biotechnology, D-07745 Jena, Germany
    EMBO J 21:3213-24. 2002
  6. ncbi Carfilzomib can induce tumor cell death through selective inhibition of the chymotrypsin-like activity of the proteasome
    Francesco Parlati
    Department of Research, Proteolix Inc, South San Francisco, CA 94080, USA
    Blood 114:3439-47. 2009
  7. ncbi Susceptibility of amyloid beta peptide degrading enzymes to oxidative damage: a potential Alzheimer's disease spiral
    Heather Shinall
    Department of Molecular and Cellular Biochemistry and the Center for Structural Biology, University of Kentucky, Lexington, Kentucky 40536, USA
    Biochemistry 44:15345-50. 2005
  8. ncbi Macromolecular chelation as an improved mechanism of protease inhibition: structure of the ecotin-trypsin complex
    M E McGrath
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0448
    EMBO J 13:1502-7. 1994
  9. ncbi Taiwan cobra chymotrypsin inhibitor: cloning, functional expression and gene organization
    Yun-Ching Cheng
    Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
    Biochim Biophys Acta 1747:213-20. 2005
  10. ncbi Modification of host erythrocyte membranes by trypsin and chymotrypsin treatments and effects on the in vitro growth of bovine and equine Babesia parasites
    Masashi Okamura
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 93:208-11. 2007

Research Grants

  1. POLYMERASE PROTEINS IN CORONAVIRUS REPLICATION
    Mark R Denison; Fiscal Year: 2010
  2. P. papatasi midgut molecules: gene function and assessing TBV candidates
    JOSE MARCELO contact RAMALHO ORTIGAO; Fiscal Year: 2010
  3. P. papatasi midgut molecules: gene function and assessing TBV candidates
    JOSE MARCELO RAMALHO ORTIGAO; Fiscal Year: 2009
  4. P. papatasi midgut molecules: gene function and assessing TBV candidates
    JOSE MARCELO contact RAMALHO ORTIGAO; Fiscal Year: 2010
  5. Tropomyosin in the antiangiogenic activity of HKa
    Keith McCrae; Fiscal Year: 2007
  6. Dengue and West Nile Viral Protease Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2005
  7. GUT ISCHEMIA & MOF SYNDROME: MICROVASCULAR TRANSDUCTION
    GREGORY BULKLEY; Fiscal Year: 1993
  8. ELECTRON MICROSCOPY OF HUMAN ALPHA-2 MACROGLOBULIN
    JAMES STOOPS; Fiscal Year: 1993
  9. Human Cathepsin G: Expression, C-Terminal Processing and Dual Specificity
    David Johnson; Fiscal Year: 2007
  10. Single Molecule Protein and Biopolymer Folding Dynamics
    Shimon Weiss; Fiscal Year: 2003

Detail Information

Publications226 found, 100 shown here

  1. ncbi Sheep mast cell proteinase-1: characterization as a member of a new class of dual-specific ruminant chymases
    A D Pemberton
    Department of Veterinary Clinical Studies, Royal Dick School of Veterinary Studies, University of Edinburgh, Roslin, Scotland, U K
    Biochem J 321:665-70. 1997
    ..bradykinin and oxidized insulin B chain were hydrolysed at P1 Phe, Leu or Tyr residues, conforming to the known chymotrypsin-like properties of the enzyme...
  2. ncbi Isolation and primary structures of seven serine proteinase inhibitors from Cyclanthera pedata seeds
    Jolanta Kowalska
    Institute of Biochemistry and Molecular Biology, University of Wrocław, Tamka 2, 50 137 Wrocław, Poland
    Biochim Biophys Acta 1760:1054-63. 2006
    ..CyPTI I-VII, were purified from ripe seeds of Cyclanthera pedata by affinity chromatography on immobilized chymotrypsin in the presence of 5 M NaCl followed by preparative native PAGE at pH 8.9...
  3. ncbi Picornaviral 3C cysteine proteinases have a fold similar to chymotrypsin-like serine proteinases
    M Allaire
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Nature 369:72-6. 1994
    ..The overall architecture of HAV-3C reveals a fold resembling that of the chymotrypsin family of serine proteinases, which is consistent with earlier predictions...
  4. ncbi A novel erythrocyte binding antigen-175 paralogue from Plasmodium falciparum defines a new trypsin-resistant receptor on human erythrocytes
    Tim-Wolf Gilberger
    Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
    J Biol Chem 278:14480-6. 2003
    ..of EBA-181 to erythrocytes differs from other members of the ebl family and is trypsin-resistant and chymotrypsin-sensitive...
  5. ncbi Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra alpha-helical domain
    Kanchan Anand
    Department of Structural Biology and Crystallography, Institute of Molecular Biotechnology, D-07745 Jena, Germany
    EMBO J 21:3213-24. 2002
    ..The active site, comprised of Cys144 and His41, is part of a chymotrypsin-like fold that is connected by a 16 residue loop to an extra domain featuring a novel alpha-helical fold...
  6. ncbi Carfilzomib can induce tumor cell death through selective inhibition of the chymotrypsin-like activity of the proteasome
    Francesco Parlati
    Department of Research, Proteolix Inc, South San Francisco, CA 94080, USA
    Blood 114:3439-47. 2009
    Carfilzomib is a proteasome inhibitor in clinical development that primarily targets the chymotrypsin-like (CT-L) subunits in both the constitutive proteasome (c20S) and the immunoproteasome (i20S)...
  7. ncbi Susceptibility of amyloid beta peptide degrading enzymes to oxidative damage: a potential Alzheimer's disease spiral
    Heather Shinall
    Department of Molecular and Cellular Biochemistry and the Center for Structural Biology, University of Kentucky, Lexington, Kentucky 40536, USA
    Biochemistry 44:15345-50. 2005
    ..Since IDE and NEP play a prominent role in the clearance of amyloid beta peptides, their oxidative inactivation and enhanced proteolysis can contribute to the onset and/or progression of Alzheimer's disease...
  8. ncbi Macromolecular chelation as an improved mechanism of protease inhibition: structure of the ecotin-trypsin complex
    M E McGrath
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0448
    EMBO J 13:1502-7. 1994
    ..This first case of protein dimerization being used to augment binding energy and allow chelation of a target protein provides a new model for protein-protein interactions and for protease inhibition...
  9. ncbi Taiwan cobra chymotrypsin inhibitor: cloning, functional expression and gene organization
    Yun-Ching Cheng
    Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
    Biochim Biophys Acta 1747:213-20. 2005
    A cDNA encoding chymotrypsin inhibitor was constructed from the cellular RNA isolated from the venom glands of Naja atra (Taiwan cobra)...
  10. ncbi Modification of host erythrocyte membranes by trypsin and chymotrypsin treatments and effects on the in vitro growth of bovine and equine Babesia parasites
    Masashi Okamura
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 93:208-11. 2007
    ..bigemina) and equine Babesia parasites (B. equi and B. caballi). The selected proteases, trypsin and chymotrypsin, clearly modified several membrane proteins of both bovine and equine RBC, as demonstrated by SDS-PAGE analysis;..
  11. ncbi Purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase from the chicken
    V Guyonnet
    Department of Poultry Science, University of Georgia, Athens 30602, USA
    J Chromatogr A 852:217-25. 1999
    The purpose of this work was to isolate, purify and partially sequence trypsin, chymotrypsin and elastase from the chicken pancreas. The extraction of the pancreatic zymogens with 0.5 M CaCl2 at pH 7...
  12. ncbi Differences in binding modes of enantiomers of 1-acetamido boronic acid based protease inhibitors: crystal structures of gamma-chymotrypsin and subtilisin Carlsberg complexes
    V S Stoll
    Department of Biochemistry, University of Toronto, 1 King s College Circle, Toronto, Ontario M5S 1A8, Canada
    Biochemistry 37:451-62. 1998
    ..has been synthesized and kinetically characterized as transition-state analog inhibitors using alpha-chymotrypsin and subtilisin Carlsberg as model systems...
  13. ncbi Immunomodulatory activity of a chymotrypsin inhibitor from Momordica cochinchinensis seeds
    Alex Yuen-Kam Tsoi
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T, Hong Kong, China
    J Pept Sci 12:605-11. 2006
    ..g. immunomodulatory effect, remain relatively unexplored. Recently, we isolated a chymotrypsin-specific inhibitor (MCoCI) from the seeds of Momordica cochinchinensis (Lour) Spreng (Family Cucurbitaceae), ..
  14. ncbi pH-responsive polymer-assisted refolding of urea- and organic solvent-denatured alpha-chymotrypsin
    I Roy
    Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India
    Protein Eng 16:1153-7. 2003
    A pH-responsive polymer Eudragit S-100 has been found to assist in correct folding of alpha-chymotrypsin denatured with 8 M urea and 100 mM dithiothreitol at pH 8.2. The complete activity could be regained within 10 min during refolding...
  15. ncbi Sequence variation in trypsin- and chymotrypsin-like cDNAs from the midgut of Ostrinia nubilalis: methods for allelic differentiation of candidate Bacillus thuringiensis resistance genes
    B S Coates
    USDA ARS, Corn Insects and Crop Genetics Research Unit, Iowa State University, Ames, IA 50010, USA
    Insect Mol Biol 15:13-24. 2006
    ..Three groups of trypsin- (OnT25, OnT23, and OnT3) and two chymotrypsin-like (OnC1 and OnC2) cDNAs were isolated from O. nubilalis midgut tissue...
  16. ncbi Purification, characterization and primary structure of a chymotrypsin inhibitor from Naja atra venom
    Xing-Ding Zhou
    Department of Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 650223 Kunming, Yunnan, PR China
    Comp Biochem Physiol B Biochem Mol Biol 137:219-24. 2004
    A chymotrypsin inhibitor, designated NA-CI, was isolated from the venom of the Chinese cobra Naja atra by three-step chromatography. It inhibited bovine alpha-chymotrypsin with a Ki of 25 nM...
  17. ncbi Identification and recombinant expression of a novel chymotrypsin from Spodoptera exigua
    Salvador Herrero
    Business Unit Bioscience, Plant Research International B V, Wageningen University and Research Centre, Wageningen, The Netherlands
    Insect Biochem Mol Biol 35:1073-82. 2005
    A novel chymotrypsin which is expressed in the midgut of the lepidopteran insect Spodoptera exigua is described. This enzyme, referred to as SeCT34, represents a novel class of chymotrypsins...
  18. ncbi An engineered chymotrypsin/cathepsin G site in domain I renders Bacillus thuringiensis Cry3A active against Western corn rootworm larvae
    Frederick S Walters
    Syngenta Biotechnology, Inc, Research Triangle Park, NC 27709 2257, USA
    Appl Environ Microbiol 74:367-74. 2008
    ..Modification of Cry3A so that it contained a chymotrypsin/cathepsin G protease recognition site in the loop between alpha-helix 3 and alpha-helix 4 of domain I, however, ..
  19. ncbi The effect of absorption enhancers on the initial degradation kinetics of insulin by alpha-chymotrypsin
    M A Radwan
    Department of Clinical Pharmacy, College of Pharmacy, Science and Medical Studies, Department for Women Students, King Saud University, P O Box 22452, 11495, Riyadh, Saudi Arabia
    Int J Pharm 217:111-20. 2001
    ..insulin absorption enhancers by following their effect on the initial kinetics of insulin incubated with alpha-chymotrypsin at 37 degrees C...
  20. ncbi The interaction of alpha-1-antitrypsin with chymotrypsin, trypsin and elastase
    A B Cohen
    Biochim Biophys Acta 391:193-200. 1975
    The mode of inhibition by alpha-1-antitrypsin of chymotrypsin, trypsin and pancreatic elastase was examined by a kinetic method...
  21. ncbi Association of novel chymotrypsin C gene variations and haplotypes in patients with chronic pancreatitis in Chinese in Taiwan
    Ming Chu Chang
    Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan
    Pancreatology 9:287-92. 2009
    Variations and haplotypes of the chymotrypsin C (CTRC) gene in Chinese patients with chronic pancreatitis (CP) and control subjects with genotype-phenotype correlation were investigated.
  22. ncbi Introduction of non-natural amino acid residues into the substrate-specific P1 position of trypsin inhibitor SFTI-1 yields potent chymotrypsin and cathepsin G inhibitors
    Anna Łegowska
    Faculty of Chemistry, University of Gdansk, Gdansk, Poland
    Bioorg Med Chem 17:3302-7. 2009
    ..Their inhibitory activities with bovine alpha-chymotrypsin and cathepsin G were estimated by determination of association equilibrium constants (K(a))...
  23. ncbi Selection of potent chymotrypsin and elastase inhibitors from M13 phage library of basic pancreatic trypsin inhibitor (BPTI)
    L Kiczak
    Lavoratory of Protein Engineering, Institute of Biochemistry and Molecular Biology, University of Wroclaw, Tamka 2, 50-137 Wroclaw, Poland
    Biochim Biophys Acta 1550:153-63. 2001
    ..pancreatic trypsin inhibitor (BPTI) that would be strong inhibitors of two serine proteinases: bovine alpha-chymotrypsin and porcine pancreatic elastase. BPTI only moderately inhibits the first and does not inhibit the second enzyme...
  24. ncbi Diffusion of alpha-chymotrypsin in solution-crowded media. A fluorescence recovery after photobleaching study
    Isabel Pastor
    Department of Physical Chemistry and Research Institute of Theoretical and Computational Chemistry of University of Barcelona, Barcelona, Spain
    J Phys Chem B 114:4028-34. 2010
    ..Here, we study the diffusion of alpha-chymotrypsin in different crowded (Dextran) in vitro solutions using a confocal laser scanning microscope...
  25. ncbi Prostate-specific antigen is a "chymotrypsin-like" serine protease with unique P1 substrate specificity
    Aaron M LeBeau
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University, Baltimore, Maryland 21231, USA
    Biochemistry 48:3490-6. 2009
    ..PSA inhibitors, therefore, may represent a promising class of therapeutics and/or imaging agents. PSA displays chymotrypsin-like specificity, cleaving after hydrophobic residues, in addition to possessing a unique ability to cleave ..
  26. ncbi Apigenin, chrysin, and luteolin selectively inhibit chymotrypsin-like and trypsin-like proteasome catalytic activities in tumor cells
    Yi Xin Wu
    Department of Biochemistry, Hamamatsu University School of Medicine, Hamamatsu, Japan
    Planta Med 76:128-32. 2010
    ..The function of proteasomes is mediated by three main catalytic activities: (1) chymotrypsin-like (CT-L), (2) trypsin-like (T-L), and (3) peptidylglutamyl peptide hydrolyzing (PGPH)...
  27. ncbi Complexation of two proteic insect inhibitors to the active site of chymotrypsin suggests decoupled roles for binding and selectivity
    A Roussel
    Architecture et Fonction de Macromolecules Biologiques, UMR 6098, CNRS et Universités d Aix Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Biol Chem 276:38893-8. 2001
    ..PMP-C and PMP-D2v) from the insect Locusta migratoria have been determined in complex with bovine alpha-chymotrypsin at 2.1- and 3.0-A resolution, respectively...
  28. ncbi Protease activity in gut of Daphnia magna: evidence for trypsin and chymotrypsin enzymes
    Eric von Elert
    Limnological Institute, University of Constance, 78434 Konstanz, Germany
    Comp Biochem Physiol B Biochem Mol Biol 137:287-96. 2004
    ..TLCK) and 4-(amidinophenyl)methanesulfonyl fluoride (APMSF) and not inhibited by chymostatin; and (ii) a chymotrypsin activity that hydrolysed synthetic chymotrypsin substrates containing more than one amino acid, did not ..
  29. ncbi Cloning and characterization of mouse klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin-like specificity
    H Matsui
    Division of Biological Sciences, Hokkaido University, Sapporo, Japan
    Eur J Biochem 267:6858-65. 2000
    ..Active recombinant mKlk27 exhibited chymotrypsin-like cleavage specificity...
  30. ncbi The Bacillus thuringiensis Cry1Aa toxin: effects of trypsin and chymotrypsin site mutations on toxicity and stability
    Aliou Bah
    Biotechnology Research Institute, National Research, Council, Montreal, Que, Canada H4P 2R2
    J Invertebr Pathol 85:120-7. 2004
    ..resistance to degradation by spruce budworm (Choristoneura fumiferana) midgut proteases by mutating potential chymotrypsin and trypsin sites...
  31. ncbi The human cytotoxic T cell granule serine protease granzyme H has chymotrypsin-like (chymase) activity and is taken up into cytoplasmic vesicles reminiscent of granzyme B-containing endosomes
    K M Edwards
    The John Connell Laboratory, The Austin Research Institute, Studley Road, Heidelberg, 3084, Australia
    J Biol Chem 274:30468-73. 1999
    ..Different granzymes display proteolytic specificities that mimic the activities of trypsin or chymotrypsin, or may cleave substrates at acidic ("Asp-ase") or at long unbranched amino acids such as Met ("..
  32. ncbi Catalytic site-specific inhibition of the 20S proteasome by 4-hydroxynonenal
    Deborah A Ferrington
    Department of Ophthalmology, University of Minnesota, Minneapolis, MN 55455, USA
    FEBS Lett 578:217-23. 2004
    ..The present study demonstrates that HNE rapidly inhibits the chymotrypsin-like activity of the 20S proteasome purified from liver...
  33. ncbi Amyloidogenic potential of alpha-chymotrypsin in different conformational states
    Nasrollah Rezaei-Ghaleh
    Department for NMR based Structural Biology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biopolymers 91:28-36. 2009
    ..In the present study, alpha-chymotrypsin, a well-known serine protease has been driven toward these structures by the use of two different conditions ..
  34. ncbi A new structural class of selective and non-covalent inhibitors of the chymotrypsin-like activity of the 20S proteasome
    C Garcia-Echeverria
    Oncology Research, Novartis Pharma Inc, Basel, Switzerland
    Bioorg Med Chem Lett 11:1317-9. 2001
    ..and in vitro characterization of a series of 2-aminobenzylstatine derivatives that inhibit non-covalently the chymotrypsin-like activity of the 20S proteasome...
  35. ncbi Structural consequences of accommodation of four non-cognate amino acid residues in the S1 pocket of bovine trypsin and chymotrypsin
    Ronny Helland
    Norwegian Structural Biology Centre, Faculty of Science, University of Troms, 9037 Troms, Norway
    J Mol Biol 333:845-61. 2003
    ..bovine pancreatic trypsin inhibitor (BPTI) variants in complex with two serine proteinases, bovine trypsin and chymotrypsin, have been determined...
  36. ncbi Crystal structure of bovine duodenase, a serine protease, with dual trypsin and chymotrypsin-like specificities
    V Z Pletnev
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences Ul, Miklukho Maklaya, Moscow, Russia
    Proteins 41:8-16. 2000
    ..4A resolution. The enzyme, which has both trypsin-like and chymotrypsin-like activities, most closely resembles human cathepsin G with which it shares 57% sequence identity and ..
  37. ncbi Trypsin and chymotrypsin inhibitors in insects and gut leeches
    Liliane Schoofs
    Laboratory of Developmental Physiology and Molecular Biology, K U Leuven, Naamsestraat 59, Leuven, B 3000 Leuven, Belgium
    Curr Pharm Des 8:483-91. 2002
    ..Also other invertebrates such as insects, which evolved from the annelids, harvest potential interesting molecules, such as serine protease inhibitors that can be exploited by the medical industry...
  38. ncbi The three-dimensional structure of the human alpha 2-macroglobulin dimer reveals its structural organization in the tetrameric native and chymotrypsin alpha 2-macroglobulin complexes
    Steven J Kolodziej
    Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 277:28031-7. 2002
    Three-dimensional electron microscopy reconstructions of the human alpha(2)-macroglobulin (alpha(2)M) dimer and chymotrypsin-transformed alpha(2)M reveal the structural arrangement of the two dimers that comprise native and proteinase-..
  39. ncbi New structural motifs on the chymotrypsin fold and their potential roles in complement factor B
    H Jing
    Center for Macromolecular Crystallography, School of Optometry, University of Alabama at Birmingham, 35294, USA
    EMBO J 19:164-73. 2000
    ..It shows a number of structural motifs novel to the chymotrypsin fold, which by sequence homology are probably present in C2 as well...
  40. ncbi Direct comparison of experimental and calculated folding free energies for hydrophobic deletion mutants of chymotrypsin inhibitor 2: free energy perturbation calculations using transition and denatured states from molecular dynamics simulations of unfoldi
    Y Pan
    Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195-7610, USA
    Biochemistry 40:2723-31. 2001
    Previous molecular dynamics (MD) simulations of thermal denaturation of chymotrypsin inhibitor 2 (CI2) have provided transition-state models in good agreement with experiment...
  41. ncbi Cloning and characterization of trypsin- and chymotrypsin-like proteases from the midgut of the sand fly vector Phlebotomus papatasi
    J M Ramalho-Ortigão
    Intracellular Parasite Biology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, 4 Center Drive, Building 4, Room 126, MSC 0425, NIH, Bethesda, MD 20892 0425, USA
    Insect Biochem Mol Biol 33:163-71. 2003
    Trypsin and chymotrypsin serine proteases are the main digestive proteases in Diptera midguts and are also involved in many aspects of the vector-parasite relationship...
  42. ncbi Chymotrypsin and neuraminidase treatment inhibits host cell invasion by Babesia divergens (Phylum Apicomplexa)
    A Zintl
    Department of Veterinary Microbiology and Parasitology, Conway Institute of Biomedical and Biomolecular Research, University College Dublin, Ireland
    Parasitology 125:45-50. 2002
    ..Parasite invasion of bovine erythrocytes was not affected by trypsin treatment while treatment with alpha-chymotrypsin led to a reduction in parasite growth of 20-40%...
  43. ncbi Alpha-chymotrypsin catalysis in imidazolium-based ionic liquids
    J A Laszlo
    New Crops and Processing Research, USDA-ARS, National Center for Agricultural Utilization Research, 1815 N. University St, Peoria, IL 61604 USA
    Biotechnol Bioeng 75:181-6. 2001
    The transesterification reaction of N-acetyl-L-phenylalanine ethyl ester with 1-propanol catalyzed by alpha-chymotrypsin was examined in the ionic liquids 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim][PF(6)]) and 1-octyl-3-..
  44. ncbi Phenotypic variation of Plasmodium falciparum merozoite proteins directs receptor targeting for invasion of human erythrocytes
    Manoj T Duraisingh
    The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Plant Cell Biology Research Centre, School of Botany, University of Melbourne 3010, Australia
    EMBO J 22:1047-57. 2003
    ..Phenotypic variation of the PfRh protein family allows P. falciparum to exploit different patterns of receptors on the erythrocyte surface and thereby respond to polymorphisms in erythrocyte receptors and to evade the host immune system...
  45. ncbi Proteasome activity in human lens nuclei and correlation with age, gender and severity of cataract
    Madeleine Zetterberg
    Institute of Clinical Neuroscience, Section of Ophthalmology, Medical Faculty, Goteborg University, Goteborg, Sweden
    Curr Eye Res 27:45-53. 2003
    ..The aim of this study was to measure proteasome activity in human lens nuclei resulting from cataract surgery and in different regions of donor lenses...
  46. ncbi First report on a potato I family chymotrypsin inhibitor from the seeds of a Cucurbitaceous plant, Momordica cochinchinensis
    Alex Y K Tsoi
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T, Hong Kong, China
    Biol Chem 385:185-9. 2004
    A 7514-Da chymotrypsin inhibitor was isolated from the seed extract of Momordica cochinchinensis (Family Cucurbitaceae) by chromatography on chymotrypsin-Sepharose 4B and subsequently by C18 reversed-phase HPLC...
  47. ncbi Role of a buried acid group in the mechanism of action of chymotrypsin
    D M Blow
    Nature 221:337-40. 1969
  48. ncbi Planktocyclin, a cyclooctapeptide protease inhibitor produced by the freshwater cyanobacterium Planktothrix rubescens
    Heike I Baumann
    Limnological Station, Institute of Plant Biology, University of Zurich, Seestrasse 187, 8802 Kilchberg, Switzerland
    J Nat Prod 70:1611-5. 2007
    ..It consists solely of proteinogenic l-amino acids and is a strong inhibitor of mammalian trypsin and alpha-chymotrypsin and a moderately active inhibitor of human recombinant caspase-8...
  49. ncbi Thermal aggregation of alpha-chymotrypsin: role of hydrophobic and electrostatic interactions
    Nasrollah Rezaei-Ghaleh
    Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
    Biophys Chem 132:23-32. 2008
    ..reported that electrostatic interactions may play a critical role in alcohol-induced aggregation of alpha-chymotrypsin (CT). In the present study, we have investigated the heat-induced aggregation of this protein...
  50. ncbi Age-dependent inhibition of proteasome chymotrypsin-like activity in the retina
    Rebecca J Kapphahn
    Department of Ophthalmology, 380 Lions Research Building, 2001 6th Street SE, University of Minnesota, Minneapolis, MN 55455, USA
    Exp Eye Res 84:646-54. 2007
    ..In contrast, the aged neural retina demonstrated a significant reduction in the chymotrypsin-like activity and decreased degradation of both casein and casein modified by 4-hydroxynonenal...
  51. ncbi Influence of modulated structural dynamics on the kinetics of alpha-chymotrypsin catalysis. Insights through chemical glycosylation, molecular dynamics and domain motion analysis
    Ricardo J Solá
    Laboratory for Applied Biochemistry and Biotechnology, Department of Chemistry, University of Puerto Rico, Rio Piedras Campus, San Juan, PR 00931
    FEBS J 273:5303-19. 2006
    Although the chemical nature of the catalytic mechanism of the serine protease alpha-chymotrypsin (alpha-CT) is largely understood, the influence of the enzyme's structural dynamics on its catalysis remains uncertain...
  52. ncbi Largamides A-H, unusual cyclic peptides from the marine cyanobacterium Oscillatoria sp
    Alberto Plaza
    Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0820, USA
    J Org Chem 71:6898-907. 2006
    ..Largamides D-G (4-7) inhibited chymotrypsin with IC(50) values ranging between 4 and 25 microM.
  53. ncbi Chymotrypsin C (CTRC) variants that diminish activity or secretion are associated with chronic pancreatitis
    Jonas Rosendahl
    Department of Gastroenterology and Hepatology, University of Leipzig, Philipp Rosenthal Strasse 27, 04103 Leipzig, Germany
    Nat Genet 40:78-82. 2008
    ..Here we have analyzed the gene encoding the trypsin-degrading enzyme chymotrypsin C (CTRC) in German subjects with idiopathic or hereditary chronic pancreatitis. Two alterations in this gene, p...
  54. ncbi Engineering of protein thermodynamic, kinetic, and colloidal stability: Chemical Glycosylation with monofunctionally activated glycans
    Ricardo J Solá
    Department of Chemistry, University of Puerto Rico, Rio Piedras Campus, San Juan 23346, Puerto Rico 00931 3346
    Biotechnol Bioeng 94:1072-9. 2006
    ..we designed and coupled monofunctional activated glycans (lactose and dextran) to the model protein alpha-chymotrypsin (alpha-CT)...
  55. ncbi A novel protease inhibitor of the alpha2-macroglobulin family expressed in the human epidermis
    Marie Florence Galliano
    UMR 5165, Epidermis Differentiation and Rheumatoid Autoimmunity, CNRS Toulouse III University IFR 30, INSERM CNRS Toulouse III University CHU, 31073 Toulouse, France
    J Biol Chem 281:5780-9. 2006
    ..Recombinant alpha2ML1 displayed inhibitory activity toward chymotrypsin, papain, thermolysin, subtilisin A, and to a lesser extent, elastase but not trypsin...
  56. ncbi Purification from Escherichia coli of a periplasmic protein that is a potent inhibitor of pancreatic proteases
    C H Chung
    J Biol Chem 258:11032-8. 1983
    ..This protein also inhibits chymotrypsin, pancreatic elastase, rat mast cell chymase, and human serosal urokinase, but it does not inhibit human ..
  57. ncbi Synthesis of alpha-chymotrypsin/polymer composites by a reverse micelle/gas antisolvent method
    Wei Li
    Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, PR China
    Colloids Surf B Biointerfaces 59:11-5. 2007
    Alpha-chymotrypsin (CT)/polyvinylpyrrolidone (PVP) composites was synthesized by combination of reverse micelles and CO(2)...
  58. ncbi Design and synthesis of enediyne-peptide conjugates and their inhibiting activity against chymotrypsin
    Sansa Dutta
    Department of Chemistry, Indian Institute of Technology, Kharagpur 721 302, India
    Bioorg Med Chem 17:3900-8. 2009
    Novel enediyne-amino acid conjugates 1-4 have been synthesized. All of these effectively target the enzyme chymotrypsin inhibiting its proteolytic activity...
  59. ncbi Active-site gating regulates substrate selectivity in a chymotrypsin-like serine protease the structure of haemophilus influenzae immunoglobulin A1 protease
    Troy A Johnson
    Department of Biochemistry and Molecular Biology, The University of Kansas Medical Center, Kansas City, KS 66160, USA
    J Mol Biol 389:559-74. 2009
    ..The N-terminal domain of the IgAP is found to possess a chymotrypsin-like fold...
  60. ncbi The sequence and reactive site of ecotin. A general inhibitor of pancreatic serine proteases from Escherichia coli
    M E McGrath
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0446
    J Biol Chem 266:6620-5. 1991
    ..a serine protease inhibitor found in the periplasm of Escherichia coli, is unusual in its ability to inhibit chymotrypsin, trypsin, and elastase...
  61. ncbi pH dependence thermal stability of a chymotrypsin inhibitor from Schizolobium parahyba seeds
    Rozeni C L Teles
    , Depto Biologia Celular, , Brasilia DF, Brazil, 70910-900
    Biophys J 88:3509-17. 2005
    The thermal stability of a Schizolobium parahyba chymotrypsin inhibitor (SPCI) as a function of pH has been investigated using fluorescence, circular dichroism, and differential scanning calorimetry (DSC)...
  62. ncbi Cyanopeptolin 954, a chlorine-containing chymotrypsin inhibitor of Microcystis aeruginosa NIVA Cya 43
    Eric von Elert
    Limnological Institute, University of Konstanz, 78457 Konstanz, Germany
    J Nat Prod 68:1324-7. 2005
    ..Cyanopeptolin 954 inhibited chymotrypsin with an IC50 value of 45 nM...
  63. ncbi Trypsin-chymotrypsin inhibitors from Vigna mungo seeds
    Allen H K Cheung
    Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Protein Pept Lett 16:277-84. 2009
    Three trypsin-chymotrypsin inhibitors were isolated from seeds of the black gram (Vigna mungo) with a procedure that entailed cation exchange chromatography on SP-Sepharose, anion exchange chromatography on Q-Sepharose, ion exchange ..
  64. ncbi Effect of ionic strength on the binding of alpha-chymotrypsin to nanoparticle receptors
    Ayush Verma
    Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA
    Langmuir 20:4178-81. 2004
    ..carboxylate-functionalized mixed monolayer protected clusters (MMPCs) effectively bind and inhibit alpha-chymotrypsin based on complementary electrostatic surface recognition...
  65. ncbi Purification and characterization of native and recombinant SaPIN2a, a plant sieve element-localized proteinase inhibitor
    Zhen Yu Wang
    State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat Sen University, Guangzhou 510275, China
    Plant Physiol Biochem 45:757-66. 2007
    ..Native SaPIN2a significantly inhibited serine proteinases such as trypsin, chymotrypsin, and subtilisin, with the most potent inhibitory activity on subtilisin...
  66. ncbi Diverse forms of Pin-II family proteinase inhibitors from Capsicum annuum adversely affect the growth and development of Helicoverpa armigera
    Vaijayanti A Tamhane
    Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Dr Homi Bhabha Road, Pune 411008 MS, India
    Gene 403:29-38. 2007
    ..CanPIs revealed that three IRD PIs have trypsin inhibitory sites, while four IRD CanPIs have both trypsin and chymotrypsin inhibitory sites...
  67. ncbi Binding of hydrophobic peptides to several non-catalytic sites promotes peptide hydrolysis by all active sites of 20 S proteasomes. Evidence for peptide-induced channel opening in the alpha-rings
    Alexei F Kisselev
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 277:22260-70. 2002
    The eukaryotic 20 S proteasome contains the following 6 active sites: 2 chymotrypsin-like, 2 trypsin-like, and 2 caspase-like...
  68. ncbi Design, synthesis and analysis of novel bicyclic and bifunctional protease inhibitors
    Agnes M Jaulent
    Department of Chemistry, Imperial College London, South Kensington Campus, London SW7 2AZ, UK
    Protein Eng Des Sel 17:681-7. 2004
    ..It is found that the new inhibitors do indeed allow bifunctional inhibition, although, unlike BBI, the small size of the inhibitor prevents the simultaneous inhibition of two proteases at the same time...
  69. ncbi Sol-gel immobilization of serine proteases for application in organic solvents
    D J van Unen
    Laboratory of Supramolecular Chemistry and Technology, MESA Research Institute, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands
    Biotechnol Bioeng 75:154-8. 2001
    The serine proteases alpha-chymotrypsin, trypsin, and subtilisin Carlsberg were immobilized in a sol-gel matrix and the effects on the enzyme activity in organic media are evaluated...
  70. ncbi Analysis of serine proteinase-inhibitor interaction by alanine shaving
    Olga Buczek
    Laboratory of Protein Engineering, Institute of Biochemistry and Molecular Biology, University of Wroclaw, 50-137 Wroclaw, Poland
    Protein Sci 11:806-19. 2002
    ..P(1) position) of bovine pancreatic trypsin inhibitor (BPTI) in interaction with two proteinases, trypsin and chymotrypsin, by a procedure called molecular shaving...
  71. ncbi Isolation and characterization of a trypsin-chymotrypsin inhibitor from the seeds of green lentil (Lens culinaris)
    Allen H K Cheung
    Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Protein Pept Lett 14:859-64. 2007
    A Bowman-Birk type trypsin-chymotrypsin inhibitor was isolated from seeds of the legume green lentil (Lens culinaris) by means of affinity chromatography on Affi-gel blue gel, ion exchange chromatography on Q-Sepharose, ion exchange ..
  72. ncbi Vinyl ester-based cyclic peptide proteasome inhibitors
    Anna Baldisserotto
    Department of Pharmaceutical Sciences and Biotechnology Center, University of Ferrara, Via Fossato di Mortara 17 19, I 44100 Ferrara, Italy
    Bioorg Med Chem Lett 18:1849-54. 2008
    ..Some of these derivatives were shown to inhibit the chymotrypsin-like activity of the proteasome at nanomolar concentration and their potency was found to depend on the size of ..
  73. ncbi Pancreatic proteolytic enzymes of ostrich purified on immobilized protein inhibitors. Characterization of a new form of chymotrypsin (Chtr1)
    Monika Zelazko
    Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Science, C K Norwida 25 27, 50 375 Wrocław, Poland
    Comp Biochem Physiol B Biochem Mol Biol 151:102-9. 2008
    Four forms of chymotrypsin (Chtr1, Chtr2, Chtr3, Chtr4), one form of trypsin and one form of elastase were purified from a slightly alkaline extract of ostrich (Struthio camelus) pancreas...
  74. ncbi Structural characterization of the fibrillar form of the yeast Saccharomyces cerevisiae prion Ure2p
    Luc Bousset
    Laboratoire d'Enzymologie et Biochimie Structurales, CNRS, 91198 Gif-sur-Yvette Cedex, France
    Biochemistry 43:5022-32. 2004
    ....
  75. ncbi Enhancement of catalytic activity of enzymes by heating in anhydrous organic solvents: 3D structure of a modified serine proteinase at high resolution
    S Sharma
    Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110 029, India
    Indian J Biochem Biophys 38:34-41. 2001
    ..Six enzymes, namely, proteinase K, wheat germ acid phosphatase, alpha-amylase, beta-glucosidase, chymotrypsin and trypsin were exposed to acetonitrile at 70 degrees C for three hr...
  76. ncbi Residues Y179 and H101 of a hydrophobic patch of factor VII are involved in activation by factor Xa
    J Jin
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Biochemistry 40:11405-10. 2001
    ....
  77. ncbi Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I
    Kentaro Shiraki
    School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan
    Eur J Biochem 269:4152-8. 2002
    ..5-10) among chymotrypsin-type serine proteases...
  78. ncbi Cationic inhibitors of serine proteinases from buckwheat seeds: study of their interaction with exogenous proteinases
    T A Tsybina
    Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia
    Biochemistry (Mosc) 69:441-4. 2004
    ..High efficiency of the inhibitors in binding bovine trypsin and chymotrypsin as well as their broad antiprotease effect, including inhibition of proteinases secreted by fungi and bacteria, ..
  79. ncbi Further insights into quinone cofactor biogenesis: probing the role of mauG in methylamine dehydrogenase tryptophan tryptophylquinone formation
    Arwen R Pearson
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Biochemistry 43:5494-502. 2004
    ..The properties of MauG, which are atypical of c-type cytochromes, are discussed in the context of these final stages of TTQ biogenesis...
  80. ncbi Higher accumulation of proteinase inhibitors in flowers than leaves and fruits as a possible basis for differential feeding preference of Helicoverpa armigera on tomato (Lycopersicon esculentum Mill, Cv. Dhanashree)
    Mrunal S Damle
    Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, Maharashtra 411 008, India
    Phytochemistry 66:2659-67. 2005
    ..Bioassay using H. armigera larvae fed on artificial diet containing tomato PIs revealed adverse effect on larval growth, pupae development, adult formation and fecundity...
  81. ncbi Ala226 to Gly and Ser189 to Asp mutations convert rat chymotrypsin B to a trypsin-like protease
    Balázs Jelinek
    Biotechnology Research Group of the Hungarian Academy of Sciences
    Protein Eng Des Sel 17:127-31. 2004
    In a previous successful attempt to convert trypsin to a chymotrypsin-like protease, 15 residues of trypsin were replaced with the corresponding ones in chymotrypsin...
  82. ncbi Noncovalent inhibition of the serine proteases, alpha-chymotrypsin and trypsin by trifluoro(organo)borates
    Reem Smoum
    Department of Pharmaceutics, School of Pharmacy, Faculty of Medicine, Hebrew University of Jerusalem, POB 12065, 91120, Jerusalem, Israel
    Org Biomol Chem 3:941-4. 2005
    ..Dixon plots showed that these compounds are reversible competitive inhibitors of alpha-chymotrypsin and trypsin...
  83. ncbi Characterization of a proteinase inhibitor from Cajanus cajan (L.)
    Soghra Khatun Haq
    Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202 002, India
    J Protein Chem 22:543-54. 2003
    ..inhibitory activity toward proteolytic enzymes belonging to the serine protease group, namely trypsin and alpha-chymotrypsin. The inhibitory activity was stable over a wide range of pH and temperatures...
  84. ncbi Reaction of lentil trypsin-chymotrypsin inhibitors with human and bovine proteinases
    Jürgen K P Weder
    Institut fur Lebensmittelchemie, Technische Universitat Munchen, Lichtenbergstrasse 4, 85748 Garching, Germany
    J Agric Food Chem 51:8045-50. 2003
    Four trypsin-chymotrypsin inhibitors from Syrian local small lentils were selected to study the reasons for their different action against human and bovine proteinases...
  85. ncbi A despecialization step underlying evolution of a family of serine proteases
    Merridee A Wouters
    Victor Chang Cardiac Research Institute, Sydney, New South Wales 2010, Australia
    Mol Cell 12:343-54. 2003
    ..This indicates that a despecialization step, characterized by broad specificity and structural plasticity, underlies evolution of new primary specificities in this protease superfamily...
  86. ncbi Highly conserved cysteines of mouse core 2 beta1,6-N-acetylglucosaminyltransferase I form a network of disulfide bonds and include a thiol that affects enzyme activity
    Ten Yang Yen
    Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132, USA
    J Biol Chem 278:45864-81. 2003
    ....
  87. ncbi Effects of black-eyed pea trypsin/chymotrypsin inhibitor on proteolytic activity and on development of Anthonomus grandis
    Octavio L Franco
    EMBRAPA Recursos Geneticos e Biotecnologia, 70770 900, Brasi lia DF, Brazil
    Phytochemistry 63:343-9. 2003
    ..Aiming to reduce the proteolytic activity, the inhibitory effects of black-eyed pea trypsin/chymotrypsin inhibitor (BTCI), towards trypsin and chymotrypsin from bovine pancreas and from midguts of A...
  88. ncbi Exposure to hydrogen peroxide diminishes NF-kappaB activation, IkappaB-alpha degradation, and proteasome activity in neutrophils
    Jaroslaw W Zmijewski
    Dept of Medicine, School of Medicine, University of Alabama, BDB 420, 1530 3rd Ave S, Birmingham, AL 35294 0012, USA
    Am J Physiol Cell Physiol 293:C255-66. 2007
    ..H(2)O(2) treatment resulted in diminished trypsin- and chymotrypsin-like proteasome activity...
  89. ncbi Proteinase inhibitors from the tropical sea anemone Radianthus macrodactylus: isolation and characteristic
    I N Sokotun
    Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia
    Biochemistry (Mosc) 72:301-6. 2007
    ..The molecular masses of RmIn I, RmIn II, and the complexes RmIn II/trypsin and RmIn I,II/alpha-chymotrypsin have been determined...
  90. ncbi Immobilized protease-assisted synthesis of engineered cysteine-knot microproteins
    Panumart Thongyoo
    Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UK
    Chembiochem 8:1107-9. 2007
  91. ncbi Proteasome-mediated degradation of tau proteins occurs independently of the chymotrypsin-like activity by a nonprocessive pathway
    Christopher Cardozo
    Department of Medicine, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Arch Biochem Biophys 408:103-10. 2002
    ..and glutamyl activities almost completely blocked tau degradation, while inhibitors active toward the chymotrypsin-like activity had minimal effects on degradation of tau and intermediates derived from it...
  92. ncbi Purification and characterization of proteinase inhibitors from wild soja (Glycine soja) seeds
    Masanobu Deshimaru
    Department of Chemistry, Faculty of Science, Fukuoka University, Japan
    Biosci Biotechnol Biochem 66:1897-903. 2002
    ..2-6.2 x 10(-9) M. Some of the inhibitors inhibited chymotrypsin and elastase as well...
  93. ncbi Examples of peptide-peptoid hybrid serine protease inhibitors based on the trypsin inhibitor SFTI-1 with complete protease resistance at the P1-P1' reactive site
    Maciej Stawikowski
    Department of Bioorganic Chemistry, Faculty of Chemistry, University of Gdansk, Sobieskiego 18 St, 80-523 Gdansk, Poland
    Chembiochem 6:1057-61. 2005
    ..The analogues synthesised appeared to be potent inhibitors of bovine beta-trypsin and alpha-chymotrypsin. These noncovalent, competitive and selective peptide-peptoid hybrid (peptomeric) inhibitors might open the way ..
  94. ncbi The sequence of sheep kappa-casein: primary structure of para-kappa A-casein
    J Jolles
    Eur J Biochem 46:127-32. 1974
  95. ncbi Kunitz-type proteinase inhibitors derived by limited proteolysis of the inter-alpha-trypsin inhibitor, VII. Characterization of the bovine inhibitor as double-headed trypsin-elastase inhibitor
    K Hochstrasser
    Hoppe Seylers Z Physiol Chem 364:1689-96. 1983
    ..The N-terminal domain is an inhibitor of bovine chymotrypsin and elastases from porcine pancreases and human polymorphonuclear granulocytes, whereas the C-terminal domain ..
  96. ncbi Alanyl-tRNA synthetase from Escherichia coli, Bombyx mori and Ratus ratus. Existence of common structural features
    J D Dignam
    Department of Biochemistry and Molecular Biology, Medical College of Ohio, Toledo 43699 0008
    Eur J Biochem 198:201-10. 1991
    ..Similar effects of substrates were observed when the enzymes were digested with chymotrypsin suggesting that the effects of substrates on protease sensitivity were not unique to trypsin...
  97. ncbi Human cytoplasmic antiproteinase neutralizes rapidly and efficiently chymotrypsin and trypsin-like proteases utilizing distinct reactive site residues
    M Riewald
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 271:14526-32. 1996
    ..were observed to form SDS-stable complexes not only with 125I-thrombin and 125I-urokinase, but also with 125I-chymotrypsin. Kinetic studies indicated that the amidolytic activity of chymotrypsin is inhibited efficiently and rapidly by ..
  98. ncbi Kunitz-type proteinase inhibitors derived by limited proteolysis of the inter-alpha-trypsin inhibitor, VII. Determination of the amino-acid sequence of the trypsin-released inhibitor from bovine inter-alpha-trypsin inhibitor
    K Hochstrasser
    Hoppe Seylers Z Physiol Chem 364:1679-87. 1983
    ..The reactive-site residue is leucine in the N-terminal domain (in the human inhibitor methionine) and arginine in the C-terminal domain in both bovine and human inhibitor...
  99. ncbi Heterologous expression of three plant serpins with distinct inhibitory specificities
    S W Dahl
    Department of Biochemistry and Nutrition, Technical University of Denmark, DK 2800 Lyngby, Denmark
    J Biol Chem 271:25083-8. 1996
    ..BSZx, BSZ4, and WSZ1 were assayed for inhibitory activity against trypsin, chymotrypsin, and cathepsin G, and cleavage sites in the reactive center loop were identified by sequencing...
  100. ncbi Unexpected crucial role of residue 225 in serine proteases
    E R Guinto
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Box 8231, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 96:1852-7. 1999
    Residue 225 in serine proteases of the chymotrypsin family is Pro or Tyr in more than 95% of nearly 300 available sequences...
  101. ncbi Matrix localization of tissue factor pathway inhibitor-2/matrix-associated serine protease inhibitor (TFPI-2/MSPI) involves arginine-mediated ionic interactions with heparin and dermatan sulfate: heparin accelerates the activity of TFPI-2/MSPI toward plas
    Y Liu
    Department of Neurosurgery, The University of Texas M D Anderson Cancer Center, Houston, Texas, 77030, USA
    Arch Biochem Biophys 370:112-8. 1999
    ..serine protease inhibitor (MSPI), a Kunitz-type serine protease inhibitor, inhibits plasmin, trypsin, chymotrypsin, plasma kallikrein, cathepsin G, and factor VIIa-tissue factor complex...

Research Grants70

  1. POLYMERASE PROTEINS IN CORONAVIRUS REPLICATION
    Mark R Denison; Fiscal Year: 2010
    ..Nsp5 possesses two chymotrypsin-like domains, whose interface comprises the active-site cavity and substrate-binding regions, and a unique ..
  2. P. papatasi midgut molecules: gene function and assessing TBV candidates
    JOSE MARCELO contact RAMALHO ORTIGAO; Fiscal Year: 2010
    ..focus on the use of RNA interference to assess the roles of proteins such as PpChym2, a blood-induced chymotrypsin that may be involved in early killing of Leishmania;and PpChit1, a midgut-specific chitinase that is thought to ..
  3. P. papatasi midgut molecules: gene function and assessing TBV candidates
    JOSE MARCELO RAMALHO ORTIGAO; Fiscal Year: 2009
    ..focus on the use of RNA interference to assess the roles of proteins such as PpChym2, a blood-induced chymotrypsin that may be involved in early killing of Leishmania; and PpChit1, a midgut-specific chitinase that is thought ..
  4. P. papatasi midgut molecules: gene function and assessing TBV candidates
    JOSE MARCELO contact RAMALHO ORTIGAO; Fiscal Year: 2010
    ..focus on the use of RNA interference to assess the roles of proteins such as PpChym2, a blood-induced chymotrypsin that may be involved in early killing of Leishmania;and PpChit1, a midgut-specific chitinase that is thought to ..
  5. Tropomyosin in the antiangiogenic activity of HKa
    Keith McCrae; Fiscal Year: 2007
    ..Finally, affinity purification of chymotrypsin-digested tropomyosin on HKa-sepharose, as w ell as panning of a cyclic random peptide Library on HKa, led to ..
  6. Dengue and West Nile Viral Protease Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2005
    ..This strategy has been successfully employed in development of chymotrypsin-and trypsin-like serine proteases and preliminary screen of DEN protease activity shows promising results...
  7. GUT ISCHEMIA & MOF SYNDROME: MICROVASCULAR TRANSDUCTION
    GREGORY BULKLEY; Fiscal Year: 1993
    ..and in cultured endothelial monolayers, these hypotheses will be tested by probing with proteases (chymotrypsin) antiproteases (soybean trypsin inhibitor), specific antioxidants (superoxide dismutuse and catalase) and ..
  8. ELECTRON MICROSCOPY OF HUMAN ALPHA-2 MACROGLOBULIN
    JAMES STOOPS; Fiscal Year: 1993
    ..Plasmin (Mr=80,000) forms a binary complex with alpha2M whereas smaller proteinases such as alpha- chymotrypsin (Mr=25,000) form a ternary complex...
  9. Human Cathepsin G: Expression, C-Terminal Processing and Dual Specificity
    David Johnson; Fiscal Year: 2007
    ..In Aim#3 the dual specificity CatG will be studied. Although CatG is a member of the chymotrypsin family, it has been shown to also cleave trypsin-like substrates...
  10. Single Molecule Protein and Biopolymer Folding Dynamics
    Shimon Weiss; Fiscal Year: 2003
    ..the proposed methodology with existing single-molecule fluorescence methodology to study the folding of protein chymotrypsin inhibitor 2 (CI2)...
  11. ANTICHYMOTRYPSIN INTERACTION WITH SERINE PROTEASES
    BARRY COOPERMAN; Fiscal Year: 1993
    ..ACT is a relatively specific inhibitory of chymotrypsin-like serine proteases [chymotrypsin (Chtr), cathepsin G (CatG)]...
  12. PROTEIN KINASE: PRIMARY STRUCTURE AND C-AMP INTERACTION
    Susan Taylor; Fiscal Year: 1980
    ..If necessary maleylated tryptic peptides, thermolysin peptides, and chymotrypsin will be isolated to establish overlapping sequences...
  13. Characterization of Pancreatic Disease in a Novel Porcine Cystic Fibrosis Model
    Aliye Uc; Fiscal Year: 2009
    ..in regulating the exocrine pancreatic function of pigs will be determined in vivo (analysis of fecal fat and chymotrypsin; collection of pancreatic fluid) and in vitro (primary porcine ductular epithelial cell cultures) using CFTR-/- ..
  14. Characterization of Pancreatic Disease in a Novel Porcine Cystic Fibrosis Model
    Aliye Uc; Fiscal Year: 2010
    ..in regulating the exocrine pancreatic function of pigs will be determined in vivo (analysis of fecal fat and chymotrypsin;collection of pancreatic fluid) and in vitro (primary porcine ductular epithelial cell cultures) using CFTR-/- ..
  15. Light Scattering & Flourescence Study of Sugar Solutions as Cryoprotective Agents
    DAVID LEE SIDEBOTTOM; Fiscal Year: 2010
    ..phase of our project, students will incorporate functional biomolecules (green fluorescent protein, GFP and chymotrypsin inhibitor 2, CI2) into these sugar solutions...
  16. Regulation of GABAA Receptor Cell Surface Expression
    NANCY LEIDENHEIMER; Fiscal Year: 2006
    ..Complementary biochemical experiments using chymotrypsin digest/Immunoblotting blotting techniques will also be used to measure receptor cell surface expression...
  17. MOLECULAR STUDIES OF HUMAN CYTOMEGALOVIRUS PROTEASE
    Liang Tong; Fiscal Year: 2003
    ..features and belongs to a new class of serine protease, distinct from the classical serine proteases such as chymotrypsin and subtilisin. Therefore, a new body of knowledge is needed on this new class of enzymes...
  18. Irreversible inhibitors of cholesterol esterase
    LORRAINE DECK; Fiscal Year: 2005
    ..will be determined at the enzyme level by screening compounds for their rates of inactivation of CE compared with the proteases chymotrypsin, trypsin and elastase, all of which are serine proteases that function within the intestine.