chymotrypsin

Summary

Summary: A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.

Top Publications

  1. doi Functional peptidomics of amphibian skin secretion: A novel Kunitz-type chymotrypsin inhibitor from the African hyperoliid frog, Kassina senegalensis
    Hui Wang
    Natural Drug Discovery group, School of Pharmacy, Queen s University, Belfast BT9 7BL, Northern Ireland, UK
    Biochimie 94:891-9. 2012
  2. pmc A mutation of the epithelial sodium channel associated with atypical cystic fibrosis increases channel open probability and reduces Na+ self inhibition
    Robert Rauh
    Department of Cellular and Molecular Physiology, Friedrich Alexander University of Erlangen Nurnberg, Germany
    J Physiol 588:1211-25. 2010
  3. doi Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom
    Ying Ying He
    Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
    Peptides 29:1692-9. 2008
  4. doi Carfilzomib can induce tumor cell death through selective inhibition of the chymotrypsin-like activity of the proteasome
    Francesco Parlati
    Department of Research, Proteolix Inc, South San Francisco, CA 94080, USA
    Blood 114:3439-47. 2009
  5. ncbi Picornaviral 3C cysteine proteinases have a fold similar to chymotrypsin-like serine proteinases
    M Allaire
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Nature 369:72-6. 1994
  6. ncbi A novel erythrocyte binding antigen-175 paralogue from Plasmodium falciparum defines a new trypsin-resistant receptor on human erythrocytes
    Tim Wolf Gilberger
    Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
    J Biol Chem 278:14480-6. 2003
  7. ncbi Chymotrypsinogen: 2.5-angstrom crystal structure, comparison with alpha-chymotrypsin, and implications for zymogen activation
    S T Freer
    Biochemistry 9:1997-2009. 1970
  8. pmc The delta-subunit of the epithelial sodium channel (ENaC) enhances channel activity and alters proteolytic ENaC activation
    Silke Haerteis
    Institut fur Zellulare und Molekulare Physiologie, Friedrich Alexander Universitat Erlangen Nurnberg, 91054 Erlangen, Germany
    J Biol Chem 284:29024-40. 2009
  9. doi Carfilzomib-dependent selective inhibition of the chymotrypsin-like activity of the proteasome leads to antitumor activity in Waldenstrom's Macroglobulinemia
    Antonio Sacco
    Medical Oncology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Clin Cancer Res 17:1753-64. 2011
  10. pmc Superactivity and conformational changes on alpha-chymotrypsin upon interfacial binding to cationic micelles
    M Soledad Celej
    Departamento de Química Biológica CIQUIBIC, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina
    Biochem J 378:1059-66. 2004

Research Grants

Detail Information

Publications280 found, 100 shown here

  1. doi Functional peptidomics of amphibian skin secretion: A novel Kunitz-type chymotrypsin inhibitor from the African hyperoliid frog, Kassina senegalensis
    Hui Wang
    Natural Drug Discovery group, School of Pharmacy, Queen s University, Belfast BT9 7BL, Northern Ireland, UK
    Biochimie 94:891-9. 2012
    ..Here we report for the first time, the isolation and structural characterisation of an inhibitor of chymotrypsin of Kunitz-type from the skin secretion of the African hyperoliid frog, Kassina senegalensis...
  2. pmc A mutation of the epithelial sodium channel associated with atypical cystic fibrosis increases channel open probability and reduces Na+ self inhibition
    Robert Rauh
    Department of Cellular and Molecular Physiology, Friedrich Alexander University of Erlangen Nurnberg, Germany
    J Physiol 588:1211-25. 2010
    ..probability (P(o)) of the mutant channel is increased, we investigated the proteolytic activation of ENaC by chymotrypsin. Single-channel recordings revealed that chymotrypsin activated near-silent channels in outside-out membrane ..
  3. doi Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom
    Ying Ying He
    Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
    Peptides 29:1692-9. 2008
    ..A novel dual Kunitz/BPTI serine proteinase inhibitor named OH-TCI (trypsin- and chymotrypsin-dual inhibitor from Ophiophagus hannah) was isolated from king cobra venom by three chromatographic steps of ..
  4. doi Carfilzomib can induce tumor cell death through selective inhibition of the chymotrypsin-like activity of the proteasome
    Francesco Parlati
    Department of Research, Proteolix Inc, South San Francisco, CA 94080, USA
    Blood 114:3439-47. 2009
    Carfilzomib is a proteasome inhibitor in clinical development that primarily targets the chymotrypsin-like (CT-L) subunits in both the constitutive proteasome (c20S) and the immunoproteasome (i20S)...
  5. ncbi Picornaviral 3C cysteine proteinases have a fold similar to chymotrypsin-like serine proteinases
    M Allaire
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Nature 369:72-6. 1994
    ..The overall architecture of HAV-3C reveals a fold resembling that of the chymotrypsin family of serine proteinases, which is consistent with earlier predictions...
  6. ncbi A novel erythrocyte binding antigen-175 paralogue from Plasmodium falciparum defines a new trypsin-resistant receptor on human erythrocytes
    Tim Wolf Gilberger
    Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
    J Biol Chem 278:14480-6. 2003
    ..of EBA-181 to erythrocytes differs from other members of the ebl family and is trypsin-resistant and chymotrypsin-sensitive...
  7. ncbi Chymotrypsinogen: 2.5-angstrom crystal structure, comparison with alpha-chymotrypsin, and implications for zymogen activation
    S T Freer
    Biochemistry 9:1997-2009. 1970
  8. pmc The delta-subunit of the epithelial sodium channel (ENaC) enhances channel activity and alters proteolytic ENaC activation
    Silke Haerteis
    Institut fur Zellulare und Molekulare Physiologie, Friedrich Alexander Universitat Erlangen Nurnberg, 91054 Erlangen, Germany
    J Biol Chem 284:29024-40. 2009
    ..Experiments exploiting the properties of the known betaS520C mutant ENaC confirmed this conclusion. Moreover, chymotrypsin had a reduced stimulatory effect on deltabetagamma-hENaC whole-cell currents compared with its effect on ..
  9. doi Carfilzomib-dependent selective inhibition of the chymotrypsin-like activity of the proteasome leads to antitumor activity in Waldenstrom's Macroglobulinemia
    Antonio Sacco
    Medical Oncology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Clin Cancer Res 17:1753-64. 2011
    ..It has been demonstrated that selective inhibition of the chymotrypsin-like (CT-L) activity of constitutive-(c20S) and immuno-(i20S) proteasome represents a valid strategy to induce ..
  10. pmc Superactivity and conformational changes on alpha-chymotrypsin upon interfacial binding to cationic micelles
    M Soledad Celej
    Departamento de Química Biológica CIQUIBIC, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina
    Biochem J 378:1059-66. 2004
    The catalytic behaviour of alpha-CT (alpha-chymotrypsin) is affected by cationic micelles of CTABr (hexadecyltrimethylammonium bromide)...
  11. doi Purification, biochemical and molecular analysis of a chymotrypsin protease with prophenoloxidase suppression activity from the entomopathogenic nematode Steinernema carpocapsae
    Natesan Balasubramanian
    CIRN and Department of Biology, University of Azores, Azores, Portugal
    Int J Parasitol 39:975-84. 2009
    A chymotrypsin serine protease (designated Sc-CHYM) was purified by gel filtration and anion-exchange chromatography from excretory-secretory products of parasitic stage Steinernema carpocapsae...
  12. pmc An engineered chymotrypsin/cathepsin G site in domain I renders Bacillus thuringiensis Cry3A active against Western corn rootworm larvae
    Frederick S Walters
    Syngenta Biotechnology, Inc, Research Triangle Park, NC 27709 2257, USA
    Appl Environ Microbiol 74:367-74. 2008
    ..Modification of Cry3A so that it contained a chymotrypsin/cathepsin G protease recognition site in the loop between alpha-helix 3 and alpha-helix 4 of domain I, however, ..
  13. ncbi Proteasome-mediated degradation of tau proteins occurs independently of the chymotrypsin-like activity by a nonprocessive pathway
    Christopher Cardozo
    Department of Medicine, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Arch Biochem Biophys 408:103-10. 2002
    ..and glutamyl activities almost completely blocked tau degradation, while inhibitors active toward the chymotrypsin-like activity had minimal effects on degradation of tau and intermediates derived from it...
  14. ncbi Identification of six chymotrypsin cDNAs from larval midguts of Helicoverpa zea and Agrotis ipsilon feeding on the soybean (Kunitz) trypsin inhibitor
    S Mazumdar-Leighton
    Department of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456, USA
    Insect Biochem Mol Biol 31:633-44. 2001
    ..Northern blots showed that some chymotrypsin mRNA were detected at higher levels while others were down-regulated when comparing insects reared on 0% STI ..
  15. ncbi Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
    Carlos E M Gomes
    Laboratório de Química e Função de Proteínas Bioativas, Departamento de Bioquimica, Centro de Biociencias, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil
    Plant Physiol Biochem 43:1095-102. 2005
    ..CpaTI was also stable from pH 2 to 12 at 37 degrees C. CpaTI weakly inhibited chymotrypsin and elastase and its inhibition of papain, a cysteine proteinase, were indicative of its bi-functionality...
  16. pmc Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra alpha-helical domain
    Kanchan Anand
    Department of Structural Biology and Crystallography, Institute of Molecular Biotechnology, D 07745 Jena, Germany
    EMBO J 21:3213-24. 2002
    ..The active site, comprised of Cys144 and His41, is part of a chymotrypsin-like fold that is connected by a 16 residue loop to an extra domain featuring a novel alpha-helical fold...
  17. ncbi Susceptibility of amyloid beta peptide degrading enzymes to oxidative damage: a potential Alzheimer's disease spiral
    Heather Shinall
    Department of Molecular and Cellular Biochemistry and the Center for Structural Biology, University of Kentucky, Lexington, Kentucky 40536, USA
    Biochemistry 44:15345-50. 2005
    ..Since IDE and NEP play a prominent role in the clearance of amyloid beta peptides, their oxidative inactivation and enhanced proteolysis can contribute to the onset and/or progression of Alzheimer's disease...
  18. ncbi Antimicrobial activity studies on a trypsin-chymotrypsin protease inhibitor obtained from potato
    Jin Young Kim
    Research Center for Proteineous Materials RCPM, Chosun University, 375 Seosuk dong, Dong Ku, Kwangju 501 759, Republic of Korea
    Biochem Biophys Res Commun 330:921-7. 2005
    A 5.6 kDa trypsin-chymotrypsin protease inhibitor was isolated from the tubers of the potato (Solanum tuberosum L cv...
  19. ncbi Largamides A-H, unusual cyclic peptides from the marine cyanobacterium Oscillatoria sp
    Alberto Plaza
    Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0820, USA
    J Org Chem 71:6898-907. 2006
    ..Largamides D-G (4-7) inhibited chymotrypsin with IC(50) values ranging between 4 and 25 microM.
  20. ncbi Taiwan cobra chymotrypsin inhibitor: cloning, functional expression and gene organization
    Yun Ching Cheng
    Institute of Biomedical Sciences, National Sun Yat Sen University, Kaohsiung 804, Taiwan
    Biochim Biophys Acta 1747:213-20. 2005
    A cDNA encoding chymotrypsin inhibitor was constructed from the cellular RNA isolated from the venom glands of Naja atra (Taiwan cobra)...
  21. pmc Active-site gating regulates substrate selectivity in a chymotrypsin-like serine protease the structure of haemophilus influenzae immunoglobulin A1 protease
    Troy A Johnson
    Department of Biochemistry and Molecular Biology, The University of Kansas Medical Center, Kansas City, KS 66160, USA
    J Mol Biol 389:559-74. 2009
    ..The N-terminal domain of the IgAP is found to possess a chymotrypsin-like fold...
  22. ncbi Purification, characterization and primary structure of a chymotrypsin inhibitor from Naja atra venom
    Xing Ding Zhou
    Department of Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 650223 Kunming, Yunnan, PR China
    Comp Biochem Physiol B Biochem Mol Biol 137:219-24. 2004
    A chymotrypsin inhibitor, designated NA-CI, was isolated from the venom of the Chinese cobra Naja atra by three-step chromatography. It inhibited bovine alpha-chymotrypsin with a Ki of 25 nM...
  23. doi PEGylated amyloid peptide nanocontainer delivery and release system
    V Castelletto
    Department of Chemistry, University of Reading, Whiteknights, Reading RG6 6AD, UK
    Langmuir 26:11624-7. 2010
    ..In the model peptide-PEG hybrid system studied, enzymatic degradation using alpha-chymotrypsin leads to selective cleavage close to the PEG-peptide linkage, break up of the micelles, and release of peptides ..
  24. doi A spider (Araneus ventricosus) chymotrypsin inhibitor that acts as an elastase inhibitor and a microbial serine protease inhibitor
    Hu Wan
    Department of Plant Protection, Huazhong Agricultural University, Wuhan, PR China
    Comp Biochem Physiol B Biochem Mol Biol 165:36-41. 2013
    ..activity and potassium channel blocking activity, but thus far, no additional roles for spider-derived chymotrypsin inhibitors have been elucidated...
  25. doi Molecular mechanisms of tolerance to cyanobacterial protease inhibitors revealed by clonal differences in Daphnia magna
    Anke Schwarzenberger
    Zoological Institute, Cologne Biocentre, University of Cologne, Zuelpicher Str 47b, 50674, Cologne, Germany
    Mol Ecol 21:4898-911. 2012
    ..Based on these findings, it is reasonable to assume that such genetic variability can also be found in natural populations and thus constitutes the basis for local adaptation of natural populations to dietary protease inhibitors...
  26. ncbi Cloning and characterization of chymotrypsin- and trypsin-like cDNAs from the gut of the Hessian fly [Mayetiola destructor (Say)]
    Yu Cheng Zhu
    USDA ARS JWDSRC, PO Box 346 141 Exp Stn Rd, Stoneville, MS 38776, USA
    Insect Biochem Mol Biol 35:23-32. 2005
    Fifteen unique cDNA clones encoding trypsin- or chymotrypsin-like proteins were cloned and characterized from a gut cDNA library derived from Hessian fly [Mayetiola destructor (Say)] larvae...
  27. ncbi The arterivirus nsp4 protease is the prototype of a novel group of chymotrypsin-like enzymes, the 3C-like serine proteases
    E J Snijder
    Department of Virology, Institute of Medical Microbiology, Leiden University, The Netherlands
    J Biol Chem 271:4864-71. 1996
    ..analysis suggested that nonstructural protein 4 (Nsp4) is a serine protease (SP) that shares properties with chymotrypsin-like enzymes belonging to two different groups...
  28. ncbi A trypsin and chymotrypsin inhibitor from Caesalpinia bonduc seeds: isolation, partial characterization and insecticidal properties
    Arindam Bhattacharyya
    Department of Botany, University of Delhi, Delhi 110 007, India
    Plant Physiol Biochem 45:169-77. 2007
    ..A 1:1 stoichiometric ratio was noted during CbTI-2-trypsin complex formation, which was absent on binding with chymotrypsin. Further, SDS-PAGE analysis also showed that CbTI-1 has affinity only towards chymotrypsin, whereas both ..
  29. ncbi Encapsulation-induced aggregation and loss in activity of gamma-chymotrypsin and their prevention
    Ingrid J Castellanos
    Department of Chemistry, University of Puerto Rico, Rio Piedras Campus, PO Box 23346, San Juan, PR 00931 3346, USA
    J Control Release 81:307-19. 2002
    ..Herein the model protein gamma-chymotrypsin has been encapsulated in poly(D,L-lactic-co-glycolic)acid (PLGA) microspheres using the solid-in-oil-in-water (..
  30. pmc Lung injury-dependent oxidative status and chymotrypsin-like activity of skeletal muscles in hamsters with experimental emphysema
    Jair Tonon
    Laboratory of Free Radicals and Pathophysiology, Department of General Pathology, Londrina, Brazil
    BMC Musculoskelet Disord 14:39. 2013
    ..Oxidative stress have been demonstrated to participate on skeletal muscle loss of several states, including disuse atrophy, mechanical ventilation, and chronic diseases. No evidences have demonstrated the occurance in a severity manner...
  31. ncbi A new approach in proteomics of wheat gluten: combining chymotrypsin cleavage and matrix-assisted laser desorption/ionization quadrupole ion trap reflectron tandem mass spectrometry
    Jiří Salplachta
    Rapid Commun Mass Spectrom 19:2725-8. 2005
  32. pmc Asparagine-linked glycosylation of human chymotrypsin C is required for folding and secretion but not for enzyme activity
    Melinda Bence
    Department of Molecular and Cell Biology, Boston University Henry M Goldman School of Dental Medicine, Boston, MA 02118, USA
    FEBS J 278:4338-50. 2011
    Human chymotrypsin C (CTRC) plays a protective role in the pancreas by mitigating premature trypsinogen activation through degradation. Mutations that abolish activity or secretion of CTRC increase the risk for chronic pancreatitis...
  33. ncbi Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity
    Andis Klegeris
    Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, British Columbia, Canada
    Glia 51:56-64. 2005
    ..Five structurally distinct inhibitors that are known to inhibit chymotrypsin-like proteases were partially protective...
  34. doi Chymotrypsin-like peptidases from Tribolium castaneum: a role in molting revealed by RNA interference
    Gunnar Broehan
    Department of Biology Chemistry, Division of Animal Physiology, University of Osnabruck, Barbarastr 11, 49069 Osnabruck, Germany
    Insect Biochem Mol Biol 40:274-83. 2010
    b>Chymotrypsin-like peptidases (CTLPs) of insects are primarily secreted into the gut lumen where they act as digestive enzymes. We studied the gene family encoding CTLPs in the genome of the red flour beetle, Tribolium castaneum...
  35. pmc Conformational changes of alpha-chymotrypsin in a fibrillation-promoting condition: a molecular dynamics study
    Nasrollah Rezaei-Ghaleh
    Institute of Biochemistry and Biophysics, Department of Cell and Molecular Biology, Faculty of Science, University of Tehran, Tehran, Iran
    Biophys J 95:4139-47. 2008
    Amyloid nanofibril formation appears to be a generic property of polypeptide chains. alpha-Chymotrypsin (aCT) was recently driven toward amyloid-like aggregation by the addition of trifluoroethanol (TFE) at intermediate concentrations...
  36. ncbi Preparation of highly active alpha-chymotrypsin for catalysis in organic media
    Ipsita Roy
    Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India
    Bioorg Med Chem Lett 14:2191-3. 2004
    A simple one step process for the preparation of free alpha-chymotrypsin, using an organic solvent to precipitate the enzyme from a buffered solution, followed by washing with organic solvents, is described...
  37. pmc Selective inhibitor of proteasome's caspase-like sites sensitizes cells to specific inhibition of chymotrypsin-like sites
    Matthew Britton
    Department of Pharmacology and Toxicology, Dartmouth Medical School, 1 Medical Center Drive, HB7936, Lebanon, NH 03756, USA
    Chem Biol 16:1278-89. 2009
    ..All eukaryotic proteasomes have three types of active sites: chymotrypsin-like, trypsin-like, and caspase-like...
  38. pmc Symplocamide A, a potent cytotoxin and chymotrypsin inhibitor from the marine Cyanobacterium Symploca sp
    Roger G Linington
    Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography and Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA 92093, USA
    J Nat Prod 71:22-7. 2008
    ..1), a potent cancer cell cytotoxin, which also inhibits serine proteases with a 200-fold greater inhibition of chymotrypsin over trypsin...
  39. ncbi alpha-Chymotrypsin inhibition studies on the lignans from Vitex negundo Linn
    Muhammad Arif Lodhi
    Dr Panjwani Center for Molecular Medicine and Drug Research, University of Karachi, Karachi 75270, Pakistan
    J Enzyme Inhib Med Chem 23:400-5. 2008
    ..The lignans (1-8) isolated from the roots of Vitex negundo Linn. were screened against the serine proteases alpha-chymotrypsin, thrombin and prolyl endopeptidase...
  40. doi Adsorption induced enzyme denaturation: the role of polymer hydrophobicity in adsorption and denaturation of alpha-chymotrypsin on allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymers
    Challa Lahari
    Biotransformations Laboratory, Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500007, India
    Langmuir 26:1096-106. 2010
    Effects of changes in hydrophobicity of polymeric support on structure and activity of alpha-chymotrypsin (E.C. 3.4.21...
  41. doi Identification and characterization of a Bowman-Birk inhibitor active towards trypsin but not chymotrypsin in Lupinus albus seeds
    Alessio Scarafoni
    Department of Agri Food Molecular Sciences, State University of Milan, Via G Celoria 2, 20133 Milano, Italy
    Phytochemistry 69:1820-5. 2008
    ..Inhibitory properties toward bovine trypsin and chymotrypsin, as well as its thermal and pH stabilities, have been also assessed...
  42. ncbi Role of endogenous cathepsin D-like and chymotrypsin-like proteolysis in human epidermal desquamation
    T Horikoshi
    Basic Research Laboratory, Kanebo Ltd, 5 3 28, Kotobuki cho, Odawara, Kanagawa 250 0002, Japan
    Br J Dermatol 141:453-9. 1999
    ..of proteinase inhibitors, whose specificity suggested that the crucial enzymatic activity at pH 8 was a chymotrypsin-like serine proteinase, while a similar activity at pH 5 was accompanied by an aspartic proteinase activity of ..
  43. ncbi The human cytotoxic T cell granule serine protease granzyme H has chymotrypsin-like (chymase) activity and is taken up into cytoplasmic vesicles reminiscent of granzyme B-containing endosomes
    K M Edwards
    The John Connell Laboratory, The Austin Research Institute, Studley Road, Heidelberg, 3084, Australia
    J Biol Chem 274:30468-73. 1999
    ..Different granzymes display proteolytic specificities that mimic the activities of trypsin or chymotrypsin, or may cleave substrates at acidic ("Asp-ase") or at long unbranched amino acids such as Met ("Met-ase")...
  44. ncbi Structure of arterivirus nsp4. The smallest chymotrypsin-like proteinase with an alpha/beta C-terminal extension and alternate conformations of the oxyanion hole
    Isabelle H Barrette-Ng
    Canadian Institutes for Health Research Group in Protein Structure and Function, Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 277:39960-6. 2002
    ..0 A resolution. Nsp4 adopts the smallest known chymotrypsin-like fold with a canonical catalytic triad of Ser-120, His-39, and Asp-65, as well as a novel alpha/beta C-..
  45. ncbi Pro- and anti-apoptotic effects of an inhibitor of chymotrypsin-like serine proteases
    Malcolm A King
    Department of Clinical Immunology, Pacific Medicine Laboratory Services, Royal North Shore Hospital, St Leonards, Australia
    Cell Cycle 3:1566-71. 2004
    The irreversible inhibitor of chymotrypsin-like serine proteases, N-tosyl-L-phenylalanine chloromethylketone (TPCK), was shown to prevent internucleosomal DNA cleavage caused by inducers of apoptosis...
  46. ncbi Fluorescence and CD spectroscopic analysis of the alpha-chymotrypsin stabilization by the ionic liquid, 1-ethyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide
    Teresa De Diego
    Departamento de Bioquímica y Biología Molecular B e Inmunología, Facultad de Quimica, Universidad de Murcia, PO Box 4021, E 30100 Murcia, Spain
    Biotechnol Bioeng 88:916-24. 2004
    The stability of alpha-chymotrypsin in the ionic liquid, 1-ethyl-3-methyl-imidizolium bis[(trifluoromethyl)sulfonyl]amide ([emim][NTf2]), was studied at 30 and 50 degrees C and compared with the stability in other liquid media, such as ..
  47. ncbi Effect of the molecular weight of poly(ethylene glycol) used as emulsifier on alpha-chymotrypsin stability upon encapsulation in PLGA microspheres
    Ingrid J Castellanos
    Department of Chemistry, University of Puerto Rico, Rio Piedras Campus, PO Box 23346, San Juan, Puerto Rico 00931 3346
    J Pharm Pharmacol 57:1261-69. 2005
    Poly(ethylene glycol) (PEG) was used as emulsifier to prepare alpha-chymotrypsin-loaded poly(lactic-co-glycolic) acid (PLGA) microspheres by a solid-in-oil-in-water (s/o/w) technique...
  48. ncbi In vivo and in vitro interactions of the Bombyx mori chymotrypsin inhibitor b1 with Escherichia coli
    Ningjia He
    Laboratories of Insect Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka, Japan
    Biosci Biotechnol Biochem 68:835-40. 2004
    Various chymotrypsin inhibitors occur in the hemolymph of silkworm larvae. Interaction of chymotrypsin inhibitor b1 (CI-b1) with Escherichia coli was examined from the viewpoint of action against invading bacteria. Injection of dead E...
  49. ncbi Modulation of the chymotrypsin-like activity of the 20S proteasome by intracellular redox status: effects of glutathione peroxidase-1 overexpression and antioxidant drugs
    Carole Kretz-remy
    Stress Oxydant, Chaperon et Apoptose, CNRS UMR 5534, Universite Claude Bernard Lyon I, 16 rue Dubois, F 69622 Villeurbanne Cedex, France
    Biol Chem 384:589-95. 2003
    ..an increased proteolytic activity toward oxidized polypeptides and the suc-LLVY-MCA peptide specific for its chymotrypsin-like activity...
  50. ncbi Crystal structures of five bovine chymotrypsin complexes with P1 BPTI variants
    Honorata Czapinska
    Laboratory of Protein Engineering, Institute of Biochemistry and Molecular Biology, University of Wroclaw, Tamka 2, 50 137 Wroclaw, Poland
    J Mol Biol 344:1005-20. 2004
    The bovine chymotrypsin-bovine pancreatic trypsin inhibitor (BPTI) interaction belongs to extensively studied models of protein-protein recognition...
  51. ncbi Detection of native peptides as potent inhibitors of enzymes. Crystal structure of the complex formed between treated bovine alpha-chymotrypsin and an autocatalytically produced fragment, IIe-Val-Asn-Gly-Glu-Glu-Ala-Val-Pro-Gly-Ser-Trp-Pro-Trp, at 2.2 ang
    Nagendra Singh
    Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110 029, India
    FEBS J 272:562-72. 2005
    b>Chymotrypsin is a prominent member of the family of serine proteases...
  52. doi A hemocyte-derived Kunitz-BPTI-type chymotrypsin inhibitor, HlChI, from the ixodid tick Haemaphysalis longicornis, plays regulatory functions in tick blood-feeding processes
    M Abdul Alim
    Laboratory of Parasitic Diseases, National Institute of Animal Health, National Agricultural and Food Research Organization, Tsukuba, Ibaraki 305 0856, Japan
    Insect Biochem Mol Biol 42:925-34. 2012
    ..Here, we describe a cDNA that encodes a functionally active chymotrypsin inhibitor of the BPTI/Kunitz family of serine protease inhibitors from the hemocytes of the ixodid tick, ..
  53. ncbi Properties and secretory mechanism of Musca domestica digestive chymotrypsin and its relation with Drosophila melanogaster homologs
    Fabio K Tamaki
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, C P 26077, 05513 970 Sao Paulo, Brazil
    Insect Biochem Mol Biol 42:482-90. 2012
    ..In-gel activity revealed a unique band against S-AAPF-MCA with the same migration as purified chymotrypsin. One chymotrypsinogen-like sequence (MdChy1) was sequenced, cloned and recombinantly expressed in Escherichia ..
  54. ncbi Chemical constituents of Cichorium intybus and their inhibitory effects against urease and alpha-chymotrypsin enzymes
    Sumayya Saied
    Department of Chemistry, University of Karachi, Karachi 75270, Pakistan
    Nat Prod Commun 6:1117-20. 2011
    ..Compounds 4-6 and 8 are reported for the first time from C. intybus. Compounds 2 and 3 showed weak inhibitory activities against urease and alpha-chymotrypsin enzymes, respectively.
  55. doi A midgut-specific chymotrypsin cDNA (Slctlp1) from Spodoptera litura: cloning, characterization, localization and expression analysis
    Qinglei Zhan
    Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China
    Arch Insect Biochem Physiol 76:130-43. 2011
    ..A full-length cDNA (Slctlp1) encoding a chymotrypsin-like serine protease was cloned from Spodoptera litura and characterized for its cDNA structure, developmental ..
  56. doi Quantitative structure-activity relationships for organophosphates binding to trypsin and chymotrypsin
    Christopher D Ruark
    Henry M Jackson Foundation for the Advancement of Military Medicine, Applied Biotechnology Branch, Wright Patterson AFB, OH 45433 5707, USA
    J Toxicol Environ Health A 74:1-23. 2011
    ..Here, QSAR were used to predict OP bimolecular rate constants for trypsin and α-chymotrypsin. A heuristic regression of over 500 topological/constitutional, geometric, thermodynamic, electrostatic, and ..
  57. doi Whole blood assay for elastase, chymotrypsin, matrix metalloproteinase-2, and matrix metalloproteinase-9 activity
    Roy B Lefkowitz
    Department of Bioengineering, University of California San Diego, La Jolla, California 92093 0448, USA
    Anal Chem 82:8251-8. 2010
    ..We now report on the development of new protease substrates for the measurement of elastase, chymotrypsin, matrix metalloproteinase (MMP)-2, and MMP-9 activity in whole blood...
  58. doi Concentration regimes and denaturation effects on the conformational changes of α-chymotrypsin by viscosity and dynamic light scattering measurements
    N Ghaouar
    Laboratoire de Physique de la Matière Molle, Faculte des Sciences de Tunis, Campus Universitaire, 2092, Tunisia
    Int J Biol Macromol 47:425-30. 2010
    This work focused on the conformational changes of α-chymotrypsin from bovine pancreas for various concentrations as well as the effects of chemical denaturation and organic solvents using both of viscosity and dynamic light scattering ..
  59. doi Functional characterization of a partial loss-of-function mutation of the epithelial sodium channel (ENaC) associated with atypical cystic fibrosis
    Regina Huber
    Institut fur Zellulare und Molekulare Physiologie, Erlangen, Germany
    Cell Physiol Biochem 25:145-58. 2010
    ..betaS520C mutant ENaC which can be converted to a channel with a P(o) of nearly one, and by experiments using chymotrypsin to proteolytically activate the channel...
  60. doi Structure and cleavage specificity of the chymotrypsin-like serine protease (3CLSP/nsp4) of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
    Xinsheng Tian
    CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
    J Mol Biol 392:977-93. 2009
    ..Thus, we provide structural evidence as well as enzymatic proof of the nsp4 protein being a functional 3CLSP. We also show that the enzyme has a strong preference for glutamic acid at the P1 position of the substrate...
  61. pmc Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation
    María Fernanda Pomares
    Departamento de Bioquimica de la Nutricion, Instituto Superior de Investigaciones Biológicas Consejo Nacional de Investigaciones Científicas y Técnicas Universidad Nacional de Tucumán, 4000 San Miguel de Tucuman, Tucuman, Argentina
    Appl Environ Microbiol 75:5734-8. 2009
    ..In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly(12) to Tyr that retained almost full antibiotic activity ..
  62. doi A trypsin-chymotrypsin inhibitor with antiproliferative activity from small glossy black soybeans
    Xiujuan Ye
    Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Planta Med 75:550-6. 2009
    ..It was adsorbed on all three ion exchangers. It inhibited trypsin with an IC(50) of 19 microM and chymotrypsin with an IC(50) of 14.3 microM...
  63. ncbi The crystal structure of guamerin in complex with chymotrypsin and the development of an elastase-specific inhibitor
    Hun Kim
    Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440 746, Korea
    J Mol Biol 376:184-92. 2008
    ..For this purpose, we determined the crystal structure of guamerin in complex with chymotrypsin at 2.5 A resolution...
  64. pmc Chymotrypsin C (caldecrin) promotes degradation of human cationic trypsin: identity with Rinderknecht's enzyme Y
    Richard Szmola
    Department of Molecular and Cell Biology, Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA
    Proc Natl Acad Sci U S A 104:11227-32. 2007
    ..Here we report that degradation of human cationic trypsin is triggered by chymotrypsin C, which selectively cleaves the Leu(81)-Glu(82) peptide bond within the Ca(2+) binding loop...
  65. ncbi Role of electrostatic interactions in 2,2,2-trifluoroethanol-induced structural changes and aggregation of alpha-chymotrypsin
    Nasrollah Rezaei-Ghaleh
    Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
    Arch Biochem Biophys 457:160-9. 2007
    It has been recently demonstrated that alpha-chymotrypsin (CT) can be driven toward amyloid aggregation by addition of 2,2,2-trifluoroethanol (TFE), at intermediate concentrations...
  66. doi Insect chymotrypsins: chloromethyl ketone inactivation and substrate specificity relative to possible coevolutional adaptation of insects and plants
    Adriana R Lopes
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, Brazil
    Arch Insect Biochem Physiol 70:188-203. 2009
    ..using different colorimetric and fluorescent substrates indicated that insect chymotrypsins differ from bovine chymotrypsin in their primary specificity toward small substrates (like N-benzoyl-L-Tyr p-nitroanilide) rather than on their ..
  67. doi Anti-elastolytic activity of a honeybee (Apis cerana) chymotrypsin inhibitor
    Bo Yeon Kim
    College of Natural Resources and Life Science, Dong A University, Busan 604 714, Republic of Korea
    Biochem Biophys Res Commun 430:144-9. 2013
    The honeybee is an important insect species in global ecology, agriculture, and alternative medicine. While chymotrypsin and trypsin inhibitors from bees show activity against cathepsin G and plasmin, respectively, no anti-elastolytic ..
  68. ncbi The arterivirus Nsp2 protease. An unusual cysteine protease with primary structure similarities to both papain-like and chymotrypsin-like proteases
    E J Snijder
    Department of Virology, Faculty of Medicine, Leiden University, The Netherlands
    J Biol Chem 270:16671-6. 1995
    ..A papain-like Cys protease in Nsp1 and a chymotrypsin-like Ser protease in Nsp4 are involved in this process...
  69. ncbi The molecular structure of the complex of Ascaris chymotrypsin/elastase inhibitor with porcine elastase
    K Huang
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Structure 2:679-89. 1994
    ..Eight different proteins from Ascaris suum have been identified as inhibitors of serine proteinases, targeting chymotrypsin, elastase and trypsin...
  70. doi Introduction of non-natural amino acid residues into the substrate-specific P1 position of trypsin inhibitor SFTI-1 yields potent chymotrypsin and cathepsin G inhibitors
    Anna Łegowska
    Faculty of Chemistry, University of Gdansk, Gdansk, Poland
    Bioorg Med Chem 17:3302-7. 2009
    ..Their inhibitory activities with bovine alpha-chymotrypsin and cathepsin G were estimated by determination of association equilibrium constants (K(a))...
  71. doi Apigenin, chrysin, and luteolin selectively inhibit chymotrypsin-like and trypsin-like proteasome catalytic activities in tumor cells
    Yi Xin Wu
    Department of Biochemistry, Hamamatsu University School of Medicine, Hamamatsu, Japan
    Planta Med 76:128-32. 2010
    ..The function of proteasomes is mediated by three main catalytic activities: (1) chymotrypsin-like (CT-L), (2) trypsin-like (T-L), and (3) peptidylglutamyl peptide hydrolyzing (PGPH)...
  72. ncbi pH-responsive polymer-assisted refolding of urea- and organic solvent-denatured alpha-chymotrypsin
    I Roy
    Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India
    Protein Eng 16:1153-7. 2003
    A pH-responsive polymer Eudragit S-100 has been found to assist in correct folding of alpha-chymotrypsin denatured with 8 M urea and 100 mM dithiothreitol at pH 8.2. The complete activity could be regained within 10 min during refolding...
  73. doi Association of novel chymotrypsin C gene variations and haplotypes in patients with chronic pancreatitis in Chinese in Taiwan
    Ming Chu Chang
    Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan
    Pancreatology 9:287-92. 2009
    Variations and haplotypes of the chymotrypsin C (CTRC) gene in Chinese patients with chronic pancreatitis (CP) and control subjects with genotype-phenotype correlation were investigated.
  74. ncbi Sequence variation in trypsin- and chymotrypsin-like cDNAs from the midgut of Ostrinia nubilalis: methods for allelic differentiation of candidate Bacillus thuringiensis resistance genes
    B S Coates
    USDA ARS, Corn Insects and Crop Genetics Research Unit, Iowa State University, Ames, IA 50010, USA
    Insect Mol Biol 15:13-24. 2006
    ..Three groups of trypsin- (OnT25, OnT23, and OnT3) and two chymotrypsin-like (OnC1 and OnC2) cDNAs were isolated from O. nubilalis midgut tissue...
  75. ncbi The effect of absorption enhancers on the initial degradation kinetics of insulin by alpha-chymotrypsin
    M A Radwan
    Department of Clinical Pharmacy, College of Pharmacy, Science and Medical Studies, Department for Women Students, King Saud University, P O Box 22452, 11495, Riyadh, Saudi Arabia
    Int J Pharm 217:111-20. 2001
    ..insulin absorption enhancers by following their effect on the initial kinetics of insulin incubated with alpha-chymotrypsin at 37 degrees C...
  76. pmc Prostate-specific antigen is a "chymotrypsin-like" serine protease with unique P1 substrate specificity
    Aaron M LeBeau
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University, Baltimore, Maryland 21231, USA
    Biochemistry 48:3490-6. 2009
    ..PSA inhibitors, therefore, may represent a promising class of therapeutics and/or imaging agents. PSA displays chymotrypsin-like specificity, cleaving after hydrophobic residues, in addition to possessing a unique ability to cleave ..
  77. ncbi Immunomodulatory activity of a chymotrypsin inhibitor from Momordica cochinchinensis seeds
    Alex Yuen Kam Tsoi
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    J Pept Sci 12:605-11. 2006
    ..g. immunomodulatory effect, remain relatively unexplored. Recently, we isolated a chymotrypsin-specific inhibitor (MCoCI) from the seeds of Momordica cochinchinensis (Lour) Spreng (Family Cucurbitaceae), ..
  78. ncbi Modification of host erythrocyte membranes by trypsin and chymotrypsin treatments and effects on the in vitro growth of bovine and equine Babesia parasites
    Masashi Okamura
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 93:208-11. 2007
    ..bigemina) and equine Babesia parasites (B. equi and B. caballi). The selected proteases, trypsin and chymotrypsin, clearly modified several membrane proteins of both bovine and equine RBC, as demonstrated by SDS-PAGE analysis;..
  79. doi Diffusion of alpha-chymotrypsin in solution-crowded media. A fluorescence recovery after photobleaching study
    Isabel Pastor
    Department of Physical Chemistry and Research Institute of Theoretical and Computational Chemistry of University of Barcelona, Barcelona, Spain
    J Phys Chem B 114:4028-34. 2010
    ..Here, we study the diffusion of alpha-chymotrypsin in different crowded (Dextran) in vitro solutions using a confocal laser scanning microscope...
  80. ncbi Purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase from the chicken
    V Guyonnet
    Department of Poultry Science, University of Georgia, Athens 30602, USA
    J Chromatogr A 852:217-25. 1999
    The purpose of this work was to isolate, purify and partially sequence trypsin, chymotrypsin and elastase from the chicken pancreas. The extraction of the pancreatic zymogens with 0.5 M CaCl2 at pH 7...
  81. ncbi The interaction of alpha-1-antitrypsin with chymotrypsin, trypsin and elastase
    A B Cohen
    Biochim Biophys Acta 391:193-200. 1975
    The mode of inhibition by alpha-1-antitrypsin of chymotrypsin, trypsin and pancreatic elastase was examined by a kinetic method...
  82. pmc Macromolecular chelation as an improved mechanism of protease inhibition: structure of the ecotin-trypsin complex
    M E McGrath
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0448
    EMBO J 13:1502-7. 1994
    ..This first case of protein dimerization being used to augment binding energy and allow chelation of a target protein provides a new model for protein-protein interactions and for protease inhibition...
  83. ncbi Identification and recombinant expression of a novel chymotrypsin from Spodoptera exigua
    Salvador Herrero
    Business Unit Bioscience, Plant Research International B V, Wageningen University and Research Centre, Wageningen, The Netherlands
    Insect Biochem Mol Biol 35:1073-82. 2005
    A novel chymotrypsin which is expressed in the midgut of the lepidopteran insect Spodoptera exigua is described. This enzyme, referred to as SeCT34, represents a novel class of chymotrypsins...
  84. ncbi Selection of potent chymotrypsin and elastase inhibitors from M13 phage library of basic pancreatic trypsin inhibitor (BPTI)
    L Kiczak
    Lavoratory of Protein Engineering, Institute of Biochemistry and Molecular Biology, University of Wroclaw, Tamka 2, 50-137 Wroclaw, Poland
    Biochim Biophys Acta 1550:153-63. 2001
    ..pancreatic trypsin inhibitor (BPTI) that would be strong inhibitors of two serine proteinases: bovine alpha-chymotrypsin and porcine pancreatic elastase. BPTI only moderately inhibits the first and does not inhibit the second enzyme...
  85. ncbi Differences in binding modes of enantiomers of 1-acetamido boronic acid based protease inhibitors: crystal structures of gamma-chymotrypsin and subtilisin Carlsberg complexes
    V S Stoll
    Department of Biochemistry, University of Toronto, 1 King s College Circle, Toronto, Ontario M5S 1A8, Canada
    Biochemistry 37:451-62. 1998
    ..has been synthesized and kinetically characterized as transition-state analog inhibitors using alpha-chymotrypsin and subtilisin Carlsberg as model systems...
  86. ncbi Cloning and characterization of trypsin- and chymotrypsin-like proteases from the midgut of the sand fly vector Phlebotomus papatasi
    J M Ramalho-Ortigão
    Intracellular Parasite Biology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, 4 Center Drive, Building 4, Room 126, MSC 0425, NIH, Bethesda, MD 20892 0425, USA
    Insect Biochem Mol Biol 33:163-71. 2003
    Trypsin and chymotrypsin serine proteases are the main digestive proteases in Diptera midguts and are also involved in many aspects of the vector-parasite relationship...
  87. ncbi Planktocyclin, a cyclooctapeptide protease inhibitor produced by the freshwater cyanobacterium Planktothrix rubescens
    Heike I Baumann
    Limnological Station, Institute of Plant Biology, University of Zurich, Seestrasse 187, 8802 Kilchberg, Switzerland
    J Nat Prod 70:1611-5. 2007
    ..It consists solely of proteinogenic l-amino acids and is a strong inhibitor of mammalian trypsin and alpha-chymotrypsin and a moderately active inhibitor of human recombinant caspase-8...
  88. pmc Age-dependent inhibition of proteasome chymotrypsin-like activity in the retina
    Rebecca J Kapphahn
    Department of Ophthalmology, 380 Lions Research Building, 2001 6th Street SE, University of Minnesota, Minneapolis, MN 55455, USA
    Exp Eye Res 84:646-54. 2007
    ..In contrast, the aged neural retina demonstrated a significant reduction in the chymotrypsin-like activity and decreased degradation of both casein and casein modified by 4-hydroxynonenal...
  89. ncbi The three-dimensional structure of the human alpha 2-macroglobulin dimer reveals its structural organization in the tetrameric native and chymotrypsin alpha 2-macroglobulin complexes
    Steven J Kolodziej
    Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 277:28031-7. 2002
    Three-dimensional electron microscopy reconstructions of the human alpha(2)-macroglobulin (alpha(2)M) dimer and chymotrypsin-transformed alpha(2)M reveal the structural arrangement of the two dimers that comprise native and proteinase-..
  90. pmc Phenotypic variation of Plasmodium falciparum merozoite proteins directs receptor targeting for invasion of human erythrocytes
    Manoj T Duraisingh
    The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Plant Cell Biology Research Centre, School of Botany, University of Melbourne 3010, Australia
    EMBO J 22:1047-57. 2003
    ..Phenotypic variation of the PfRh protein family allows P. falciparum to exploit different patterns of receptors on the erythrocyte surface and thereby respond to polymorphisms in erythrocyte receptors and to evade the host immune system...
  91. ncbi Role of a buried acid group in the mechanism of action of chymotrypsin
    D M Blow
    Nature 221:337-40. 1969
  92. ncbi Trypsin and chymotrypsin inhibitors in insects and gut leeches
    Liliane Schoofs
    Laboratory of Developmental Physiology and Molecular Biology, K U Leuven, Naamsestraat 59, Leuven, B 3000 Leuven, Belgium
    Curr Pharm Des 8:483-91. 2002
    ..Also other invertebrates such as insects, which evolved from the annelids, harvest potential interesting molecules, such as serine protease inhibitors that can be exploited by the medical industry...
  93. ncbi Purification from Escherichia coli of a periplasmic protein that is a potent inhibitor of pancreatic proteases
    C H Chung
    J Biol Chem 258:11032-8. 1983
    ..This protein also inhibits chymotrypsin, pancreatic elastase, rat mast cell chymase, and human serosal urokinase, but it does not inhibit human ..
  94. pmc Chymotrypsin C (CTRC) variants that diminish activity or secretion are associated with chronic pancreatitis
    Jonas Rosendahl
    Department of Gastroenterology and Hepatology, University of Leipzig, Philipp Rosenthal Strasse 27, 04103 Leipzig, Germany
    Nat Genet 40:78-82. 2008
    ..Here we have analyzed the gene encoding the trypsin-degrading enzyme chymotrypsin C (CTRC) in German subjects with idiopathic or hereditary chronic pancreatitis. Two alterations in this gene, p...
  95. ncbi Thermal aggregation of alpha-chymotrypsin: role of hydrophobic and electrostatic interactions
    Nasrollah Rezaei-Ghaleh
    Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
    Biophys Chem 132:23-32. 2008
    ..reported that electrostatic interactions may play a critical role in alcohol-induced aggregation of alpha-chymotrypsin (CT). In the present study, we have investigated the heat-induced aggregation of this protein...
  96. ncbi Proteasome activity in human lens nuclei and correlation with age, gender and severity of cataract
    Madeleine Zetterberg
    Institute of Clinical Neuroscience, Section of Ophthalmology, Medical Faculty, Goteborg University, Goteborg, Sweden
    Curr Eye Res 27:45-53. 2003
    ..The aim of this study was to measure proteasome activity in human lens nuclei resulting from cataract surgery and in different regions of donor lenses...
  97. ncbi Cyanopeptolin 954, a chlorine-containing chymotrypsin inhibitor of Microcystis aeruginosa NIVA Cya 43
    Eric von Elert
    Limnological Institute, University of Konstanz, 78457 Konstanz, Germany
    J Nat Prod 68:1324-7. 2005
    ..Cyanopeptolin 954 inhibited chymotrypsin with an IC50 value of 45 nM...
  98. ncbi First report on a potato I family chymotrypsin inhibitor from the seeds of a Cucurbitaceous plant, Momordica cochinchinensis
    Alex Y K Tsoi
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    Biol Chem 385:185-9. 2004
    A 7514-Da chymotrypsin inhibitor was isolated from the seed extract of Momordica cochinchinensis (Family Cucurbitaceae) by chromatography on chymotrypsin-Sepharose 4B and subsequently by C18 reversed-phase HPLC...
  99. ncbi Effect of ionic strength on the binding of alpha-chymotrypsin to nanoparticle receptors
    Ayush Verma
    Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA
    Langmuir 20:4178-81. 2004
    ..carboxylate-functionalized mixed monolayer protected clusters (MMPCs) effectively bind and inhibit alpha-chymotrypsin based on complementary electrostatic surface recognition...
  100. pmc pH dependence thermal stability of a chymotrypsin inhibitor from Schizolobium parahyba seeds
    Rozeni C L Teles
    Universidade de Brasilia, Depto Biologia Celular, Laboratorio de Biofisica, Brasilia DF, Brazil, 70910 900
    Biophys J 88:3509-17. 2005
    The thermal stability of a Schizolobium parahyba chymotrypsin inhibitor (SPCI) as a function of pH has been investigated using fluorescence, circular dichroism, and differential scanning calorimetry (DSC)...
  101. ncbi The Bacillus thuringiensis Cry1Aa toxin: effects of trypsin and chymotrypsin site mutations on toxicity and stability
    Aliou Bah
    Biotechnology Research Institute, National Research, Council, Montreal, Que, Canada H4P 2R2
    J Invertebr Pathol 85:120-7. 2004
    ..resistance to degradation by spruce budworm (Choristoneura fumiferana) midgut proteases by mutating potential chymotrypsin and trypsin sites...

Research Grants70

  1. POLYMERASE PROTEINS IN CORONAVIRUS REPLICATION
    Mark R Denison; Fiscal Year: 2010
    ..Nsp5 possesses two chymotrypsin-like domains, whose interface comprises the active-site cavity and substrate-binding regions, and a unique ..
  2. P. papatasi midgut molecules: gene function and assessing TBV candidates
    JOSE MARCELO contact RAMALHO ORTIGAO; Fiscal Year: 2010
    ..focus on the use of RNA interference to assess the roles of proteins such as PpChym2, a blood-induced chymotrypsin that may be involved in early killing of Leishmania;and PpChit1, a midgut-specific chitinase that is thought to ..
  3. P. papatasi midgut molecules: gene function and assessing TBV candidates
    JOSE MARCELO RAMALHO ORTIGAO; Fiscal Year: 2009
    ..focus on the use of RNA interference to assess the roles of proteins such as PpChym2, a blood-induced chymotrypsin that may be involved in early killing of Leishmania; and PpChit1, a midgut-specific chitinase that is thought ..
  4. P. papatasi midgut molecules: gene function and assessing TBV candidates
    JOSE MARCELO contact RAMALHO ORTIGAO; Fiscal Year: 2010
    ..focus on the use of RNA interference to assess the roles of proteins such as PpChym2, a blood-induced chymotrypsin that may be involved in early killing of Leishmania;and PpChit1, a midgut-specific chitinase that is thought to ..
  5. Tropomyosin in the antiangiogenic activity of HKa
    Keith McCrae; Fiscal Year: 2007
    ..Finally, affinity purification of chymotrypsin-digested tropomyosin on HKa-sepharose, as w ell as panning of a cyclic random peptide Library on HKa, led to ..
  6. Dengue and West Nile Viral Protease Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2005
    ..This strategy has been successfully employed in development of chymotrypsin-and trypsin-like serine proteases and preliminary screen of DEN protease activity shows promising results...
  7. GUT ISCHEMIA & MOF SYNDROME: MICROVASCULAR TRANSDUCTION
    GREGORY BULKLEY; Fiscal Year: 1993
    ..and in cultured endothelial monolayers, these hypotheses will be tested by probing with proteases (chymotrypsin) antiproteases (soybean trypsin inhibitor), specific antioxidants (superoxide dismutuse and catalase) and ..
  8. Human Cathepsin G: Expression, C-Terminal Processing and Dual Specificity
    David Johnson; Fiscal Year: 2007
    ..In Aim#3 the dual specificity CatG will be studied. Although CatG is a member of the chymotrypsin family, it has been shown to also cleave trypsin-like substrates...
  9. ELECTRON MICROSCOPY OF HUMAN ALPHA-2 MACROGLOBULIN
    JAMES STOOPS; Fiscal Year: 1993
    ..Plasmin (Mr=80,000) forms a binary complex with alpha2M whereas smaller proteinases such as alpha- chymotrypsin (Mr=25,000) form a ternary complex...
  10. Single Molecule Protein and Biopolymer Folding Dynamics
    Shimon Weiss; Fiscal Year: 2003
    ..the proposed methodology with existing single-molecule fluorescence methodology to study the folding of protein chymotrypsin inhibitor 2 (CI2)...
  11. ANTICHYMOTRYPSIN INTERACTION WITH SERINE PROTEASES
    BARRY COOPERMAN; Fiscal Year: 1993
    ..ACT is a relatively specific inhibitory of chymotrypsin-like serine proteases [chymotrypsin (Chtr), cathepsin G (CatG)]...
  12. PROTEIN KINASE: PRIMARY STRUCTURE AND C-AMP INTERACTION
    Susan Taylor; Fiscal Year: 1980
    ..If necessary maleylated tryptic peptides, thermolysin peptides, and chymotrypsin will be isolated to establish overlapping sequences...
  13. Characterization of Pancreatic Disease in a Novel Porcine Cystic Fibrosis Model
    Aliye Uc; Fiscal Year: 2009
    ..in regulating the exocrine pancreatic function of pigs will be determined in vivo (analysis of fecal fat and chymotrypsin; collection of pancreatic fluid) and in vitro (primary porcine ductular epithelial cell cultures) using CFTR-/- ..
  14. Characterization of Pancreatic Disease in a Novel Porcine Cystic Fibrosis Model
    Aliye Uc; Fiscal Year: 2010
    ..in regulating the exocrine pancreatic function of pigs will be determined in vivo (analysis of fecal fat and chymotrypsin;collection of pancreatic fluid) and in vitro (primary porcine ductular epithelial cell cultures) using CFTR-/- ..
  15. Light Scattering & Flourescence Study of Sugar Solutions as Cryoprotective Agents
    DAVID LEE SIDEBOTTOM; Fiscal Year: 2010
    ..phase of our project, students will incorporate functional biomolecules (green fluorescent protein, GFP and chymotrypsin inhibitor 2, CI2) into these sugar solutions...
  16. Regulation of GABAA Receptor Cell Surface Expression
    NANCY LEIDENHEIMER; Fiscal Year: 2006
    ..Complementary biochemical experiments using chymotrypsin digest/Immunoblotting blotting techniques will also be used to measure receptor cell surface expression...
  17. MOLECULAR STUDIES OF HUMAN CYTOMEGALOVIRUS PROTEASE
    Liang Tong; Fiscal Year: 2003
    ..features and belongs to a new class of serine protease, distinct from the classical serine proteases such as chymotrypsin and subtilisin. Therefore, a new body of knowledge is needed on this new class of enzymes...
  18. Irreversible inhibitors of cholesterol esterase
    LORRAINE DECK; Fiscal Year: 2005
    ..will be determined at the enzyme level by screening compounds for their rates of inactivation of CE compared with the proteases chymotrypsin, trypsin and elastase, all of which are serine proteases that function within the intestine.