Summary: Enzymes that catalyze the degradation of collagen by acting on the peptide bonds. EC 3.4.24.-.

Top Publications

  1. ncbi Interstitial collagenases as markers of tumor progression
    C E Brinckerhoff
    Department of Medicine, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Clin Cancer Res 6:4823-30. 2000
  2. pmc Collagen degradation in the abdominal aneurysm: a conspiracy of matrix metalloproteinase and cysteine collagenases
    Hazem Abdul-Hussien
    Department of Vascular Surgery, Leiden University Medical Center, Leiden, The Netherlands
    Am J Pathol 170:809-17. 2007
  3. pmc Postnatal expression in hyaline cartilage of constitutively active human collagenase-3 (MMP-13) induces osteoarthritis in mice
    L A Neuhold
    Molecular Genetics Division, Wyeth Ayerst Research, Princeton, New Jersey, USA
    J Clin Invest 107:35-44. 2001
  4. ncbi SLRP interaction can protect collagen fibrils from cleavage by collagenases
    Yeqing Geng
    Genetics Unit, Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, Quebec, Canada H3G 1A6
    Matrix Biol 25:484-91. 2006
  5. ncbi Regulated expression of collagenases MMP-1, -8, and -13 and stromelysins MMP-3, -10, and -11 by human corneal epithelial cells
    De Quan Li
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 44:2928-36. 2003
  6. ncbi The role of endogenous versus exogenous tPA on edema formation in murine ICH
    Ruth Thiex
    Department of Neurosurgery, Aachen University, Aachen, Germany
    Exp Neurol 189:25-32. 2004
  7. ncbi Evidence for a protective role for adiponectin in osteoarthritis
    Tsu Hsin Chen
    Department of Orthopedics Surgery, Shin Kong WHS Memorial Hospital, Taipei, Taiwan
    Biochim Biophys Acta 1762:711-8. 2006
  8. ncbi Tuftsin fragment 1-3 is beneficial when delivered after the induction of intracerebral hemorrhage
    Jian Wang
    Department of Pharmacological Sciences, University Medical Center at Stony Brook, Stony Brook, NY 11794 8651, USA
    Stroke 36:613-8. 2005
  9. ncbi Protective role of tuftsin fragment 1-3 in an animal model of intracerebral hemorrhage
    Jian Wang
    Department of Pharmacological Sciences, University Medical Center at Stony Brook, Stony Brook, NY 11794 8651, USA
    Ann Neurol 54:655-64. 2003
  10. pmc Collagen and collagenase gene expression in three-dimensional collagen lattices are differentially regulated by alpha 1 beta 1 and alpha 2 beta 1 integrins
    O Langholz
    Department of Dermatology, University of Cologne, Germany
    J Cell Biol 131:1903-15. 1995

Detail Information

Publications350 found, 100 shown here

  1. ncbi Interstitial collagenases as markers of tumor progression
    C E Brinckerhoff
    Department of Medicine, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Clin Cancer Res 6:4823-30. 2000
    ..and MMP-9, have been given considerable attention for their roles in invasion and metastasis, the interstitial collagenases, a subfamily of MMPs that cleaves the stromal collagens types I and III, have received relatively little ..
  2. pmc Collagen degradation in the abdominal aneurysm: a conspiracy of matrix metalloproteinase and cysteine collagenases
    Hazem Abdul-Hussien
    Department of Vascular Surgery, Leiden University Medical Center, Leiden, The Netherlands
    Am J Pathol 170:809-17. 2007
    ..Collagen turnover critically depends on specific collagenases that cleave the triple helical region of fibrillar collagen...
  3. pmc Postnatal expression in hyaline cartilage of constitutively active human collagenase-3 (MMP-13) induces osteoarthritis in mice
    L A Neuhold
    Molecular Genetics Division, Wyeth Ayerst Research, Princeton, New Jersey, USA
    J Clin Invest 107:35-44. 2001
    ..These results demonstrate that excessive MMP-13 activity can result in articular cartilage degradation and joint pathology of the kind observed in OA, suggesting that excessive activity of this proteinase can lead to this disease...
  4. ncbi SLRP interaction can protect collagen fibrils from cleavage by collagenases
    Yeqing Geng
    Genetics Unit, Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, Quebec, Canada H3G 1A6
    Matrix Biol 25:484-91. 2006
    ..Interaction with either recombinant decorin, fibromodulin or lumican results in decreased collagenase cleavage of both fibril types. Thus SLRP interaction can help protect collagen fibrils from cleavage by collagenases.
  5. ncbi Regulated expression of collagenases MMP-1, -8, and -13 and stromelysins MMP-3, -10, and -11 by human corneal epithelial cells
    De Quan Li
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 44:2928-36. 2003
    This study investigated the regulated expression of collagenases (MMP-1, -8, and -13) and stromelysins (MMP-3, -10, and -11) by human corneal epithelial cells treated with IL-1 beta, TNF-alpha, and doxycycline, a medication used to treat ..
  6. ncbi The role of endogenous versus exogenous tPA on edema formation in murine ICH
    Ruth Thiex
    Department of Neurosurgery, Aachen University, Aachen, Germany
    Exp Neurol 189:25-32. 2004
    ..In conclusion, the role of the endogenous tPA appears to be limited to the early phase of edema formation, whereas exogenous rtPA is edema-promoting between days 3 and 7 after ICH...
  7. ncbi Evidence for a protective role for adiponectin in osteoarthritis
    Tsu Hsin Chen
    Department of Orthopedics Surgery, Shin Kong WHS Memorial Hospital, Taipei, Taiwan
    Biochim Biophys Acta 1762:711-8. 2006
    ..Together these findings clearly indicate that the adiponectin may act as a protective role in the progression of OA, and this also provide new thinking on the relationship between obesity and OA...
  8. ncbi Tuftsin fragment 1-3 is beneficial when delivered after the induction of intracerebral hemorrhage
    Jian Wang
    Department of Pharmacological Sciences, University Medical Center at Stony Brook, Stony Brook, NY 11794 8651, USA
    Stroke 36:613-8. 2005
    ..In this study, we investigate the protection and efficacy of treatment when MIF is administered 2 hours after collagenase injection...
  9. ncbi Protective role of tuftsin fragment 1-3 in an animal model of intracerebral hemorrhage
    Jian Wang
    Department of Pharmacological Sciences, University Medical Center at Stony Brook, Stony Brook, NY 11794 8651, USA
    Ann Neurol 54:655-64. 2003
    ..Our results suggest that endogenous tPA assists in the clearance of intracerebral hemorrhage, presumably by affecting microglial activation, and MIF could be a valuable neuroprotective agent for the treatment of ICH...
  10. pmc Collagen and collagenase gene expression in three-dimensional collagen lattices are differentially regulated by alpha 1 beta 1 and alpha 2 beta 1 integrins
    O Langholz
    Department of Dermatology, University of Cologne, Germany
    J Cell Biol 131:1903-15. 1995
    ..The data presented here indicate that different signaling pathways exist leading to the three events discussed here, and that these pathways do not per se depend upon each other...
  11. ncbi Tissue inhibitor of metalloproteinase-1 messenger RNA expression is enhanced relative to interstitial collagenase messenger RNA in experimental liver injury and fibrosis
    J P Iredale
    University of Medicine, Southampton General Hospital, Southampton, England
    Hepatology 24:176-84. 1996
    ..We suggest that increased expression of TIMP-1 relative to interstitial collagenase by HSCs may promote progression of liver fibrosis in these rat models by preventing degradation of secreted collagens...
  12. pmc Tumor cell traffic through the extracellular matrix is controlled by the membrane-anchored collagenase MT1-MMP
    Farideh Sabeh
    Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA
    J Cell Biol 167:769-81. 2004
    ..Thus, MT1-MMP serves as the major cell-associated proteinase necessary to confer normal or neoplastic cells with invasive activity...
  13. ncbi Isolation and expansion of synovial CD34(-)CD44(+)CD90(+) mesenchymal stem cells: comparison of an enzymatic method and a direct explant technique
    Dae Hee Lee
    Department of Orthopaedic Surgery, Korea University College of Medicine, Seoul, Korea
    Connect Tissue Res 52:226-34. 2011
    ..The conventional method of MSC isolation involves single-cell suspensions using collagenases. Recently, a nonenzymatic explant technique was developed to isolate MSCs...
  14. ncbi Collagenase for enzymatic debridement: a systematic review
    Janet Ramundo
    Emory University Wound Ostomy and Continence Nursing Education Program, Atlanta, Georgia, USA
    J Wound Ostomy Continence Nurs 36:S4-11. 2009
    ..A preponderance of evidence from this systematic review confirms that collagenase ointment is a safe and effective choice for debridement of cutaneous ulcers and burn wounds...
  15. ncbi Gauging recovery after hemorrhagic stroke in rats: implications for cytoprotection studies
    Crystal L MacLellan
    Department of Psychology, University of Alberta, Edmonton, Alberta, Canada
    J Cereb Blood Flow Metab 26:1031-42. 2006
    ..Such testing must be based on factors including the type of lesion, the postoperative delay and the time required to complete testing...
  16. ncbi Polycystic kidney disease-like domains of clostridial collagenases and their role in collagen recruitment
    Ulrich Eckhard
    Division of Structural Biology, Department of Molecular Biology, University of Salzburg, Billrothstraße 11, A 5020 Salzburg, Austria
    Biol Chem 392:1039-45. 2011
    Bacterial collagenases exhibit a multimodular domain organization...
  17. ncbi Carbamylation differentially alters type I collagen sensitivity to various collagenases
    Stéphane Jaisson
    Laboratory of Biochemistry and Molecular Biology, CNRS UMR 6198, IFR53 Biomolecules, Faculty of Medicine, University of Reims Champagne Ardenne, 51 rue Cognacq Jay, 51095 Reims Cedex, France
    Matrix Biol 26:190-6. 2007
    ..Degradation assays revealed that carbamylated collagen exhibited a greater resistance to collagenases (i.e...
  18. ncbi A low temperature method of isolating normal human articular chondrocytes
    N C Hidvegi
    Department of Plastic Surgery, Royal Free Hospital School of Medicine, London, UK
    Osteoarthritis Cartilage 14:89-93. 2006
    ..Our objective was to develop an efficient method of isolating chondrocytes from NHAC without jeopardising the viability of these cells...
  19. ncbi Expression of interstitial collagenase during skeletal development of the mouse is restricted to osteoblast-like cells and hypertrophic chondrocytes
    S Gack
    Forschungszentrum Karlsruhe, Institut fur Genetik, Germany
    Cell Growth Differ 6:759-67. 1995
  20. ncbi Heparin-enhanced zymographic detection of matrilysin and collagenases
    W H Yu
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33101, USA
    Anal Biochem 293:38-42. 2001
    Unlike the gelatinases (MMP-2 and -9), matrilysin (MMP-7) and collagenases (MMP-1 and -13) are difficult to detect at low levels in conventional casein or gelatin zymography...
  21. ncbi Matrix metalloproteinase 9 (92-kDa gelatinase/type IV collagenase) from HT 1080 human fibrosarcoma cells. Purification and activation of the precursor and enzymic properties
    Y Okada
    Department of Pathology, School of Medicine, Kanazawa University, Japan
    J Biol Chem 267:21712-9. 1992
    ..These results indicate that MMP-9 has different activation mechanisms and substrate specificity from those of MMP-2 (72-kDa gelatinase/type IV collagenase)...
  22. pmc Experimental model of warfarin-associated intracerebral hemorrhage
    Christian Foerch
    Neuroprotection Research Laboratory, Massachusetts General Hospital, Harvard Medical School, Charlestown, Masschusetts 02129, USA
    Stroke 39:3397-404. 2008
    ..This study describes the development of a mouse model of W-ICH in which hematoma growth and outcomes can be correlated with anticoagulation parameters...
  23. pmc Genetically engineered resistance for MMP collagenases promotes abdominal aortic aneurysm formation in mice infused with angiotensin II
    Jun o Deguchi
    Cardiovascular Division, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Lab Invest 89:315-26. 2009
    ..Optical analysis further indicated altered collagen fiber orientation in the adventitia of Col(R/R)/apoE(-/-) mice. These results demonstrate that collagen content regulates aortic biomechanical properties and influences AAA formation...
  24. ncbi Unbalanced collagenases/TIMP-1 expression and epithelial apoptosis in experimental lung fibrosis
    Victor Ruiz
    Instituto Nacional de Enfermedades Respiratorias, Mexico City, DF, CP 14080, Mexico
    Am J Physiol Lung Cell Mol Physiol 285:L1026-36. 2003
    ..Our results showed a significant decrease of collagenases MMP-8 and MMP-13, with an increase of TIMP-1 and transforming growth factor-beta...
  25. ncbi Cleavage of lumican by membrane-type matrix metalloproteinase-1 abrogates this proteoglycan-mediated suppression of tumor cell colony formation in soft agar
    Yingyi Li
    Department of Molecular Virology and Oncology and Center for the Development of Molecular Target Drugs, Cancer Research Institute, Kanazawa University, Kanazawa, Ishikawa, Japan
    Cancer Res 64:7058-64. 2004
    ..These results suggest that MT1-MMP abrogates lumican-mediated suppression of tumor cell colony formation in soft agar by degrading this proteoglycan, which down-regulates it through the induction of p21...
  26. ncbi Intracerebral hemorrhage models in rat: comparing collagenase to blood infusion
    Crystal L MacLellan
    Department of Psychology and Centre for Neuroscience, University of Alberta, Edmonton, Alberta, Canada
    J Cereb Blood Flow Metab 28:516-25. 2008
    ..Thus, both should be used whenever possible taking into account the significant differences between these models and their limitations. Furthermore, this work illustrates significant weaknesses with several outcome measures...
  27. ncbi Increase in neurogenesis and neuroblast migration after a small intracerebral hemorrhage in rats
    Tadashi Masuda
    Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, Nagoya 467 8601, Japan
    Neurosci Lett 425:114-9. 2007
    ..These data suggest that collagenase-induced ICH enhances the proliferation of endogenous NPCs and the migration of newly born neuroblasts toward the hemorrhage area...
  28. ncbi TGF-beta1 stimulates production of gelatinase (MMP-9), collagenases (MMP-1, -13) and stromelysins (MMP-3, -10, -11) by human corneal epithelial cells
    Hyun Seung Kim
    The Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin Street, NC 205, Houston, TX 77030, USA
    Exp Eye Res 79:263-74. 2004
    ..This study investigated the regulated expression of gelatinases (MMP-2 and -9), collagenases (MMP-1 and -13) and stromelysins (MMP-3, -10, and -11) by TGF-beta1 in cultured human corneal epithelial cells...
  29. pmc Orientation within the exosporium and structural stability of the collagen-like glycoprotein BclA of Bacillus anthracis
    Jeremy A Boydston
    UAB Department of Microbiology, BBRB 409, 1530 3rd Ave S, Birmingham, AL 35294 2170, USA
    J Bacteriol 187:5310-7. 2005
    ..Structural data indicate that the CTD is composed of multiple beta strands. Taken together, our results suggest that BclA and particularly its CTD form a rugged shield around the spore...
  30. ncbi The assessment of cartilage degradation in vivo: development of an immunoassay for the measurement in body fluids of type II collagen cleaved by collagenases
    A Robin Poole
    Joint Diseases Laboratory, Shriners Hospitals for Children, Montreal, Quebec, Canada
    J Immunol Methods 294:145-53. 2004
    ..developed which recognizes a neoepitope in type II collagen which is generated by the intrahelical cleavage of collagenases. Antibody reactivity is directed at the carboxyl-terminus of the TCA or 3/4 piece of the degraded alpha1(II) ..
  31. ncbi Matrix metalloproteinase and proinflammatory cytokine production by chondrocytes of human osteoarthritic cartilage: associations with degenerative changes
    L C Tetlow
    University Department of Medicine, Manchester Royal Infirmary, The University of Manchester, UK
    Arthritis Rheum 44:585-94. 2001
    ..RESULTS: Immunodetection of the collagenases (MMPs 1, 8, and 13) and stromelysin 1 (MMP-3) was demonstrated in a proportion of chondrocytes in the ..
  32. ncbi Zymography: a single-step staining method for quantitation of proteolytic activity on substrate gels
    T M Leber
    Imperial Cancer Research Fund London, United Kingdom
    Anal Biochem 249:24-8. 1997
    ..This improved protocol is fast and reproducible. Its linear range of almost two log scales permits assay of proteolytic activity in a wider range than current methods...
  33. ncbi Extracellular collagenases and the endocytic receptor, urokinase plasminogen activator receptor-associated protein/Endo180, cooperate in fibroblast-mediated collagen degradation
    Daniel H Madsen
    Finsen Laboratory, Rigshospitalet, Ole Maaløes Vej 5, DK 2200 Copenhagen N, Denmark
    J Biol Chem 282:27037-45. 2007
  34. pmc A universal strategy for high-yield production of soluble and functional clostridial collagenases in E. coli
    Paulina Ducka
    Department of Molecular Biology, University of Salzburg, Austria
    Appl Microbiol Biotechnol 83:1055-65. 2009
    Clostridial collagenases are foe and friend: on the one hand, these enzymes enable host infiltration and colonization by pathogenic clostridia, and on the other hand, they are valuable biotechnological tools due to their capacity to ..
  35. pmc Collagenase 3 is a target of Cbfa1, a transcription factor of the runt gene family involved in bone formation
    M J Jimenez
    Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain
    Mol Cell Biol 19:4431-42. 1999
  36. ncbi Isolation and expansion of adipose-derived stem cells for tissue engineering
    Trine Fink
    Department of Health Science and Technology, Aalborg University, Aalborg, Denmark
    Front Biosci (Elite Ed) 3:256-63. 2011
    ..In this study, we show that it is possible to maximize cell yield regardless of donor individual characteristics by simple manipulations of media composition, cell seeding density and gaseous environment...
  37. pmc Collagenase-3 (matrix metalloproteinase-13) expression is induced in oral mucosal epithelium during chronic inflammation
    V J Uitto
    Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, Canada
    Am J Pathol 152:1489-99. 1998
    ..Thus, it is likely that MMP-13 expression is instrumental in the subepithelial collagenolysis and local invasion of the activated mucosal epithelium into the connective tissue...
  38. ncbi Collagenase increases the transcapillary pressure gradient and improves the uptake and distribution of monoclonal antibodies in human osteosarcoma xenografts
    Live Eikenes
    Department of Physics and Department of Circulation and Medical Imaging, The Norwegian University of Science and Technology, Høgskoleringen 5, 7491 Trondheim, Norway
    Cancer Res 64:4768-73. 2004
  39. ncbi Endotoxin-mediated delayed islet graft function is associated with increased intra-islet cytokine production and islet cell apoptosis
    T Berney
    Diabetes Research Institute, University of Miami School of Medicine, FL 33136, USA
    Transplantation 71:125-32. 2001
    ..This emphasizes the fact that using endotoxin-free reagents during isolation is a key factor for successful islet transplantation...
  40. ncbi Rapid determination of substrate specificity of Clostridium histolyticum beta-collagenase using an immobilized peptide library
    Yongbo Hu
    Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, Kansas 66045, USA
    J Biol Chem 277:8366-71. 2002
    ..The immobilized peptide library proved to be a powerful approach for assessing the substrate specificity of C. histolyticum beta-collagenase, so it may be applied to the study of other proteases of interest...
  41. ncbi Cleavage of fibromodulin in cartilage explants involves removal of the N-terminal tyrosine sulfate-rich region by proteolysis at a site that is sensitive to matrix metalloproteinase-13
    Terrence F Heathfield
    Department of Cell and Molecular Biology, Section for Connective Tissue Biology, Lund University, SE 22184 Lund, Sweden
    J Biol Chem 279:6286-95. 2004
  42. ncbi Collagenolytic proteases from bacteria
    K Watanabe
    Department of Applied Biochemistry, Kyoto Prefectural University, Shimogamo, Sakyo, 606 8522 Kyoto, Japan
    Appl Microbiol Biotechnol 63:520-6. 2004
    ..However, bacterial collagenolytic proteases have many interesting and useful features. This review focuses on the collagenolytic proteases from bacteria, in particular their molecular properties and practical applications...
  43. ncbi Matrix metalloproteinases and their inhibitors in tumour growth and invasion
    V M Kahari
    Department of Dermatology, Turku University Central Hospital, University of Turku, Finland
    Ann Med 31:34-45. 1999
    ..In this review we discuss the role of MMPs and their inhibitors in tumour invasion as a basis for prognostic purposes and for targeted therapeutic intervention in cancer...
  44. ncbi Kinetics of hematoma expansion in murine warfarin-associated intracerebral hemorrhage
    Sergio Illanes
    Department of Neurology, University Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany
    Brain Res 1320:135-42. 2010
    ..Herein, we refine a mouse model of OAC-ICH using repetitive T2* MRI to describe kinetics of hematoma enlargement, and establish a benchside point of care INR assay (PoC) for assessment of anticoagulation...
  45. ncbi Impaired glutathione reductase activity and levels of collagenase and elastase in synovial fluid in rheumatoid arthritis
    L Bazzichi
    Rheumatic Diseases Unit, Medica Santa Chiara Hospital, University of Pisa, Pisa, Italy
    Clin Exp Rheumatol 20:761-6. 2002
  46. pmc Cloning of the gene for interstitial collagenase-3 (matrix metalloproteinase-13) from rabbit synovial fibroblasts: differential expression with collagenase-1 (matrix metalloproteinase-1)
    M P Vincenti
    Department of Medicine, Dartmouth Medical School, Hanover, NH, 03755, USA
    Biochem J 331:341-6. 1998
    ..and transient than that of MMP-1, suggesting that distinct mechanisms regulate the expression of these two collagenases. We have cloned the rabbit MMP-13 gene from synovial fibroblasts and demonstrated that the rabbit gene shares ..
  47. ncbi Maturational disturbance of chondrocytes in Cbfa1-deficient mice
    M Inada
    Department of Medicine III, Osaka University Medical School, Japan
    Dev Dyn 214:279-90. 1999
    ..These findings demonstrate that Cbfa1 is an important factor for chondrocyte differentiation...
  48. ncbi Comparative analysis of highly defined proteases for the isolation of adipose tissue-derived stem cells
    Linda Pilgaard
    Laboratory for Stem Cell Research, Aalborg University, Fredrik Bajers Vej 3B, 9220 Aalborg, Denmark
    Regen Med 3:705-15. 2008
    ..Before the potential of adipose tissue-derived stem cells can fully be exploited for a broad scope of tissue-engineering and cell-based therapeutical applications, an effective and reproducible method for isolation is needed...
  49. ncbi Collagen dissolution by keratinocytes requires cell surface plasminogen activation and matrix metalloproteinase activity
    Sarah Netzel-Arnett
    Matrix Metalloproteinase Unit, NIDCR, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:45154-61. 2002
    ..We propose that plasminogen activation facilitates keratinocyte-mediated collagen breakdown via the direct activation of matrix metalloproteinase-13 and possibly other fibrillar collagenases.
  50. ncbi Insulin-like growth factor-I improves cellular and molecular aspects of healing in a collagenase-induced model of flexor tendinitis
    Linda A Dahlgren
    Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Veterinary Medical Center, Ithaca, NY 14853, USA
    J Orthop Res 20:910-9. 2002
    ..Considered together with the decreased soft tissue swelling and improved sonographic healing, these data support the potential use of intralesional IGF-I for treatment of debilitating tendon injuries...
  51. pmc MMP-9/gelatinase B is a key regulator of growth plate angiogenesis and apoptosis of hypertrophic chondrocytes
    T H Vu
    Department of Anatomy, University of California, San Francisco 94143, USA
    Cell 93:411-22. 1998
    ..Growth plates from gelatinase B-null mice in culture show a delayed release of an angiogenic activator, establishing a role for this proteinase in controlling angiogenesis...
  52. ncbi Structure of full-length porcine synovial collagenase reveals a C-terminal domain containing a calcium-linked, four-bladed beta-propeller
    J Li
    Blackert Laboratory, Imperial College, London, UK
    Structure 3:541-9. 1995
    The collagenases are members of the family of zinc-dependent enzymes known as the matrix metalloproteinases (MMPs)...
  53. ncbi SV40-transformed human lung fibroblasts secrete a 92-kDa type IV collagenase which is identical to that secreted by normal human macrophages
    S M Wilhelm
    Division of Dermatology, Washington University School of Medicine, St Louis, Missouri 63110
    J Biol Chem 264:17213-21. 1989
  54. ncbi Localized unfolding of collagen explains collagenase cleavage near imino-poor sites
    Collin M Stultz
    Harvard MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA
    J Mol Biol 319:997-1003. 2002
    b>Collagenases cleave all three chains of type III collagen at specific sites characterized by a Gly-Leu or a Gly-Ile bond that is upstream from an imino acid-poor region...
  55. ncbi Collagenase-3 binds to a specific receptor and requires the low density lipoprotein receptor-related protein for internalization
    O Y Barmina
    Department of Pharmacological Science, St Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 274:30087-93. 1999
  56. ncbi Expression of collagenase-3 (MMP-13) enhances invasion of human fibrosarcoma HT-1080 cells
    Risto Ala-aho
    Centre for Biotechnology, University of Turku and Abo Akademi University, FIN 20520 Turku, Finland
    Int J Cancer 97:283-9. 2002
    ..Our data provide direct evidence for the role of MMP-13 as a potent invasion proteinase, which alone can enhance the ability of malignant cells to penetrate through both basement membrane and fibrillar collagen...
  57. ncbi Influence of neutral protease activity on human islet isolation outcome
    H Brandhorst
    Third Medical Department, University Hospital, Giessen, Germany
    Transplant Proc 37:241-2. 2005
    ..Since no information is available about the effect of NP activity on islet release from the human pancreas, the present study evaluated the effect of various NP concentrations on the outcome of human islet isolation...
  58. ncbi The HBZ factor of human T-cell leukemia virus type I dimerizes with transcription factors JunB and c-Jun and modulates their transcriptional activity
    Jihane Basbous
    Laboratoire Infections Retrovirales et Signalisation Cellulaire, CNRS Université Montpellier I, Unité Mixte de Recherche 5121 Institut Fédératif de Recherche 122, Institut de Biologie, 4 Boulevard Henri IV, 34960 Montpellier Cedex 2, France
    J Biol Chem 278:43620-7. 2003
    ..Our results support the hypothesis that HBZ could be a negative modulator of the Tax effect by controlling Tax expression at the transcriptional level and by attenuating activation of AP-1 by Tax...
  59. ncbi Embryogenesis of the rat heart: the expression of collagenases
    Anna Ratajska
    The Medical University of Warsaw, Department of Pathological Anatomy, Chalubinskiego, Poland
    Basic Res Cardiol 97:189-97. 2002
  60. pmc Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage
    R C Billinghurst
    Department of Surgery, McGill University, Montreal, Quebec, Canada
    J Clin Invest 99:1534-45. 1997
    ..A secondary cleavage followed the initial cleavage produced by these recombinant collagenases. This generated neoepitope COL2-1/4N2...
  61. ncbi Effect of the pancreatic digestion with liberase versus collagenase on the yield, function and viability of neonatal rat pancreatic islets
    Ayman Hyder
    Faculty of Science, Damietta 34517, Egypt
    Cell Biol Int 29:831-4. 2005
    ..The insulin synthesis (content) tended also to be better in liberase-isolated islets. It could be concluded that liberase could be non-significantly preferred in the isolation of neonatal rat islets in comparison with collagenase...
  62. ncbi Elucidating the function of non catalytic domains of collagenases and aggrecanases
    Hideaki Nagase
    Matrix Biology Department, Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College London, London, United Kingdom
    Connect Tissue Res 49:169-74. 2008
    ..Among them, collagenases are unique as they have an ability to degrade triple helical interstitial collagens into 3/4 and 1/4 fragments, ..
  63. ncbi Incomplete digestion preserves chondrocytes from dedifferentiating in long-termed culture on plastic substrate
    Kai Liu
    Second Affiliated Hospital of Medical College of Xi an Jiaotong University, Xi an, Shanxi 710004, China
    Tissue Cell 41:1-11. 2009
    ..Especially, as extracellular matrix was preserved, chondrocytes expressed stable phenotype in a rather long-termed culture...
  64. ncbi Nonsimultaneous administration of pancreas dissociation enzymes during islet isolation
    Tatsuya Kin
    Clinical Islet Transplant Program, University of Alberta and Alberta Health Services, Edmonton, Alberta, Canada
    Transplantation 87:1700-5. 2009
    ..However, this conversion significantly affected the field in transplant activity. Here, we report our successful conversion from Liberase to Serva collagenase with the use of a modified digestion protocol...
  65. ncbi Cerebral angiogenesis after collagenase-induced intracerebral hemorrhage in rats
    Tao Tang
    Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, PR China
    Brain Res 1175:134-42. 2007
  66. pmc High expression of 92-kD type IV collagenase (gelatinase B) in the osteoclast lineage during mouse development
    P Reponen
    Biocenter, University of Oulu, Finland
    J Cell Biol 124:1091-1102. 1994
  67. ncbi Proteinase expression in early mouse embryos is regulated by leukaemia inhibitory factor and epidermal growth factor
    M B Harvey
    Department of Medical Biochemistry, University of Calgary, Alberta, Canada
    Development 121:1005-14. 1995
  68. pmc A retinoic acid receptor agonist Am80 rescues neurons, attenuates inflammatory reactions, and improves behavioral recovery after intracerebral hemorrhage in mice
    Hideaki Matsushita
    Department of Chemico Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    J Cereb Blood Flow Metab 31:222-34. 2011
    ..Moreover, Am80-treated mice exhibited better recovery from hemorrhage-induced neurologic deficits than vehicle-treated mice. These results suggest that RAR is a promising target of neuroprotective therapy for ICH...
  69. pmc Experience of islet isolation without neutral protease supplementation
    Tatsuya Kin
    Clinical Islet Laboratory and Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada
    Islets 2:278-82. 2010
    ..As a result NP was not added at all for these isolations (no-NP group). In the remaining 84 isolations, NP was added during digestion phase (control group)...
  70. ncbi Contrasting effects of fluoroquinolone antibiotics on the expression of the collagenases, matrix metalloproteinases (MMP)-1 and -13, in human tendon-derived cells
    A N Corps
    Rheumatology Research Unit, Box 194, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2QQ, UK
    Rheumatology (Oxford) 44:1514-7. 2005
    ..We have now tested additional fluoroquinolones and investigated whether they have a similar effect on expression of MMP-13...
  71. ncbi Natural and synthetic retinoids afford therapeutic effects on intracerebral hemorrhage in mice
    Hideaki Matsushita
    Department of Chemico Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    Eur J Pharmacol 683:125-31. 2012
    ..ATRA and Am80 also significantly attenuated damage of axon tracts as revealed by amyloid precursor protein immunohistochemistry. These results underscore potential therapeutic values of NR1B agonists for intracerebral hemorrhage...
  72. ncbi In vivo evidence for active matrix metalloproteinases in human endometrium supports their role in tissue breakdown at menstruation
    Jin Zhang
    Prince Henry s Institute of Medical Research, Clayton, Victoria 3168, Australia
    J Clin Endocrinol Metab 87:2346-51. 2002
    ..This is the first in vivo evidence for increased active MMPs in menstrual endometrium compared with other stages of the cycle. These findings position the MMPs for a critical role in the matrix degradation at menstruation...
  73. ncbi α7 Nicotinic acetylcholine receptor agonist attenuates neuropathological changes associated with intracerebral hemorrhage in mice
    M Hijioka
    Department of Chemico Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5 1 Oe honmachi, Chuo Ku, Kumamoto 862 0973, Japan
    Neuroscience 222:10-9. 2012
    ..These results suggest that α7 nAChR agonist can provide neuroprotective effect on ICH-induced injury, independently of its anti-inflammatory actions...
  74. ncbi Purification and properties of individual collagenases from Streptomyces sp. strain 3B
    D Petrova
    Faculty of Biology, University of Sofia, St Kliment Ohridski, 1164 Sofia, Bulgaria
    Folia Microbiol (Praha) 51:93-8. 2006
    ..Purification is described here leading to two collagenases (I and II) with specific activity of 3350 and 3600 U/mg, respectively, the highest activity obtained as yet for ..
  75. ncbi Insulin-like growth factor I-triggered cell migration and invasion are mediated by matrix metalloproteinase-9
    E Mira
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Spain
    Endocrinology 140:1657-64. 1999
    ..Based on these results, we suggest that IGF-I-induced MCF-7 cell migration is mediated by the MMP-9 activity on the cell surface and by alpha(v)beta5 integrin...
  76. pmc Expression of matrix metalloproteinase-1, -2 and -3 in squamous cell carcinoma and actinic keratosis
    R Tsukifuji
    Division of Dermatology, National Chiba Hospital, Japan
    Br J Cancer 80:1087-91. 1999
    ..Moreover, MMP-1 expression could be an early event in the development of SCC, and AK demonstrating MMP-1 mRNA, might be in a more advanced dysplastic state, progressing to SCC...
  77. ncbi Simultaneous determination of matrix metalloproteinase (MMP)-7, MMP-1, -3, and -13 gene expression by multiplex PCR in colorectal carcinomas
    Elke Roeb
    Medical Clinic III, Rheinisch Westfälische Technische Hochschule Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany
    Int J Colorectal Dis 19:518-24. 2004
    ..The aim of this study was to evaluate MMP-7 gene expression in comparison with MMP-1, MMP-3, and MMP-13 in patients with resectable rectal and colon cancer by a semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR)...
  78. ncbi Protein kinase A dependent phosphorylation activates Mg2+ efflux in the basolateral region of the liver
    C Cefaratti
    Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106 4970, USA
    Mol Cell Biochem 297:209-14. 2007
    ..These data suggest that phosphorylation of the Na(+)/Mg(2+) exchanger or a nearby protein activates the Mg(2+) transport mechanism in hepatocytes...
  79. ncbi Relaxin positively regulates matrix metalloproteinase expression in human lower uterine segment fibroblasts using a tyrosine kinase signaling pathway
    S Palejwala
    Department of Obstetrics, Gynecology, and Women's Health, New Jersey Medical School, Newark, New Jersey 07103, USA
    Endocrinology 142:3405-13. 2001
  80. pmc Local conformation and dynamics of isoleucine in the collagenase cleavage site provide a recognition signal for matrix metalloproteinases
    Jianxi Xiao
    Department of Chemistry and Chemical Biology, BioMaPS Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA
    J Biol Chem 285:34181-90. 2010
  81. ncbi Evidence to suggest that cathepsin K degrades articular cartilage in naturally occurring equine osteoarthritis
    T Vinardell
    Departement des Sciences Cliniques, Faculte de Medecine Veterinaire, Universite de Montreal, St Hyacinthe, Quebec, Canada
    Osteoarthritis Cartilage 17:375-83. 2009
    ..This exposes a neoepitope that can now be identified by specific antibodies. The aim of this study was to obtain evidence suggesting a role for CK in naturally occurring equine OA in both lesional and peri-lesional regions...
  82. ncbi Flow cytometric analysis of gelatinase B (MMP-9) activity using immobilized fluorescent substrate on microspheres
    Y St-Pierre
    Immunology Research Center, Institut Armand Frappier, Universite du Quebec, Laval, Canada
    Cytometry 25:374-80. 1996
  83. pmc Exosite interactions impact matrix metalloproteinase collagen specificities
    Trista K Robichaud
    Department of Periodontics, University of Texas Health Science Center, San Antonio, Texas 78229, USA
    J Biol Chem 286:37535-42. 2011
    ..Further characterization of exosite determinants that govern interactions of MMPs with collagenous substrates should aid the development of pharmacotherapeutics that target individual MMPs...
  84. ncbi Interleukin-17F affects cartilage matrix turnover by increasing the expression of collagenases and stromelysin-1 and by decreasing the expression of their inhibitors and extracellular matrix components in chondrocytes
    Shihoko Tanigawa
    Nihon University Graduate School of Dentistry, 1 8 13, Kanda Surugadai, Chiyoda ku, Tokyo 101 8310, Japan
    Cytokine 56:376-86. 2011
    ..Our results suggest that IL-17F stimulates cartilage degradation by increasing the expression of collagenases (MMP-1 and -13) and stromelysin-1 (MMP-3) and by decreasing expression of their inhibitors (TIMP-2 and -4), ..
  85. ncbi Stabilization of type I collagen against collagenases (type I) and thermal degradation using iron complex
    Nishtar Nishad Fathima
    Chemical Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, India
    J Inorg Biochem 100:1774-80. 2006
    ..Since THPS is reducing in nature, it is expected to keep iron in the +2 state and if THP chelates to Fe(II), the hydrolytic behavior of iron can also be controlled...
  86. ncbi Structural implications for the role of the N terminus in the 'superactivation' of collagenases. A crystallographic study
    P Reinemer
    Max Planck Institut fur Biochemie, Abteilung für Strukturforschung, Martinsried, Germany
    FEBS Lett 338:227-33. 1994
    For the collagenases PMNL-CL and FIB-CL, the presence of the N-terminal Phe79 correlates with an increase in proteolytic activity...
  87. pmc Transcriptional repression of the human collagenase-1 (MMP-1) gene in MDA231 breast cancer cells by all-trans-retinoic acid requires distal regions of the promoter
    U Benbow
    Department of Medicine, Dartmouth Medical School, Hanover NH 03755, USA
    Br J Cancer 79:221-8. 1999
    ..We conclude that collagenase-1 repression in MDA231 cells occurs by a novel regulatory pathway that does not depend on the proximal AP-1 site at -73 bp, but does depend on distal regions in the collagenase-1 promoter...
  88. ncbi Decreased brain edema after collagenase-induced intracerebral hemorrhage in mice lacking the inducible nitric oxide synthase gene. Laboratory investigation
    Dong Wook Kim
    Department of Pediatrics, Ilsan Paik Hospital, Inje University, College of Medicine, Goyang, Korea
    J Neurosurg 111:995-1000. 2009
    ..The authors tested whether iNOS would influence hematoma size and brain edema after ICH...
  89. ncbi Kinetic analysis of matrix metalloproteinase activity using fluorogenic triple-helical substrates
    J L Lauer-Fields
    Department of Chemistry and Biochemistry, Florida Atlantic University, 777 Glades Road, Boca Raton, Florida 33431-0991, USA
    Biochemistry 40:5795-803. 2001
    ..This indicates that fluorogenic triple-helical substrates mimic the behavior of the native collagen substrate and may be useful for the investigation of collagenase triple-helical activity...
  90. ncbi NF-kappa B regulation by I kappa B kinase-2 in rheumatoid arthritis synoviocytes
    K Aupperle
    Division of Rheumatology, Allergy and Immunology, University of California San Diego School of Medicine, La Jolla, CA, USA
    J Immunol 166:2705-11. 2001
    ..These data indicate that IKK2 is the key convergence pathway for cytokine-induced NF-kappaB activation. Furthermore, IKK2 regulates adhesion molecule, matrix metalloproteinase, and cytokine production in FLS...
  91. pmc Crystal structure of an active form of human MMP-1
    Shalini Iyer
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK
    J Mol Biol 362:78-88. 2006 matrix metalloproteinases with collagenolytic activity by MMP-1, MMP-8 and MMP-13, collectively known as the collagenases. Matrix metalloproteinase 1 (MMP-1) plays a pivotal role in degradation of interstitial collagen types I, II, ..
  92. ncbi Effects of mechanical tension on matrix degradation by human periodontal ligament cells cultured in collagen gels
    J W Von den Hoff
    Department of Orthodontics and Oral Biology, College of Dental Science, University Medical Centre Nijmegen, Nijmegen, The Netherlands
    J Periodontal Res 38:449-57. 2003
    ..Periodontal ligament (PDL) cells are thought to play a crucial role in the remodelling of periodontal tissues during orthodontic tooth movement...
  93. ncbi In vitro sensitivity of the three mammalian collagenases to tetracycline inhibition: relationship to bone and cartilage degradation
    R A Greenwald
    Department of Medicine Rheumatology, Long Island Jewish Medical Center, New Hyde Park, NY 11040
    Bone 22:33-8. 1998
  94. ncbi Assessment of human islet isolation with four different collagenases
    M Shimoda
    Division of Cardiology, Department of Internal Medicine, Baylor University Medical Center, Baylor Heart and Vascular Institute, Dallas, Texas, USA
    Transplant Proc 42:2049-51. 2010
    ..Liberase HI had been used as a standard preparation until the issues concerning bovine spongiform encephalopathy. Thus, we must now use other collagenases for clinical islet transplantation, four of which we have evaluated herein.
  95. ncbi Expression and induction of collagenases (MMP-8 and -13) in plasma cells associated with bone-destructive lesions
    J Wahlgren
    Faculty of Medicine and Biomedicum, University of Helsinki, Finland
    J Pathol 194:217-24. 2001
    ..of immunoglobulin-producing plasma cells, typically present in sites of chronic inflammation, to express collagenases (MMP-8 and -13) in vivo and in vitro...
  96. ncbi Cellular mechanisms for human procollagenase-3 (MMP-13) activation. Evidence that MT1-MMP (MMP-14) and gelatinase a (MMP-2) are able to generate active enzyme
    V Knauper
    Department of Cell and Molecular Biology, Strangeways Research Laboratory, Worts Causeway, Cambridge, CB1 4RN, United Kingdom
    J Biol Chem 271:17124-31. 1996
    ..This new activation cascade may be significant in human breast cancer pathology, where all three enzymes have been implicated as playing important roles...
  97. ncbi Apoptosis and gene expression of collagenases but not gelatinases in rabbit disc fragment cultures
    Daniel Haschtmann
    MEM Research Center for Orthopaedic Surgery, Institute for Surgical Technology and Biomechanics, University of Bern, Switzerland
    J Neurosurg Spine 8:552-60. 2008
  98. pmc Human 72-kilodalton type IV collagenase forms a complex with a tissue inhibitor of metalloproteases designated TIMP-2
    G I Goldberg
    Division of Dermatology, Washington University School of Medicine, Saint Louis, MO 63110
    Proc Natl Acad Sci U S A 86:8207-11. 1989
    ..Both activity and activation of the complex can be completely inhibited by further addition of stoichiometric quantities of purified TIMP-2 or recombinant TIMP...
  99. pmc Prostaglandin PGE2 at very low concentrations suppresses collagen cleavage in cultured human osteoarthritic articular cartilage: this involves a decrease in expression of proinflammatory genes, collagenases and COL10A1, a gene linked to chondrocyte hypert
    Elena V Tchetina
    Shriners Hospitals for Children, Department of Surgery, McGill University, 1529 Cedar Avenue, Montreal, Quebec H3G 1A6, Canada
    Arthritis Res Ther 9:R75. 2007 cultured human osteoarthritic cartilage has been shown to be associated with decreased expression of collagenases, cytokines, genes associated with chondrocyte hypertrophy, and upregulation of prostaglandin (PG)E2 production...
  100. ncbi Molecular cloning and expression of collagenase-3, a novel human matrix metalloproteinase produced by breast carcinomas
    J M Freije
    Departamento de Biología Funcional and Morfología y Biología Celular, Universidad de Ovideo, Spain
    J Biol Chem 269:16766-73. 1994
    ..since it represents the third member of this subfamily, composed at present of fibroblast and neutrophil collagenases. The collagenase-3 cDNA was expressed in a vaccinia virus system, and the recombinant protein was able to ..
  101. ncbi Collagenases in cancer
    Risto Ala-aho
    Department of Medical Biochemistry and Molecular Biology, and MediCity Research Laboratory, University of Turku, Turku, Finland
    Biochimie 87:273-86. 2005
    Three mammalian collagenases (MMP-1, MMP-8, and MMP-13) belong to family of matrix metalloproteinases and are the principal secreted endopeptidases capable of cleaving collagenous extracellular matrix...

Research Grants65

  1. The Role of Adipocyte-Induced Inflammation in Prostate Tumor Progression
    ..Ultimately, these studies should lead to identification of novel signaling pathways and molecular mechanisms predisposing prostate cells to metastasize to bone. ..
  2. Nuclear Events in PTH Action on Bone
    Nicola C Partridge; Fiscal Year: 2013
    ..Moreover, it will define how HDACs contribute to this. In so doing, the data will also provide new perspectives into treatment of disorders of calcium metabolism, cancer-associated bone disease and other bone disorders. ..
  3. Role of DDR2 in OA-like Pathogenesis in Osteochondrodysplasias
    Yefu Li; Fiscal Year: 2010
    ..This study should not only provide novel insights into the role of DDR2 in the progression of genetic forms of OA, but may shed light on aspects of disease progression in non-genetic forms as well. ..
  4. Collagenase degradation in extracellular matrix in aging
    Gary J Fisher; Fiscal Year: 2010
  5. Abdominal Adipose Tissue Inflammation
    Peter S Tobias; Fiscal Year: 2013
    ..Collectively, these studies will provide key insight into an extreme atherosclerosis pathology linked to systemic inflammation resulting from chronic exposure to tissue injury, infectious agents and pathogens. ..
  6. The role of TNF-Alpha in cutaneous integrity
    Victoria P Werth; Fiscal Year: 2013
    ..will determine the roles of TNFa in UV-induced collagen change and inflammation by comparing the activation of collagenases and related enzymes, as well as populations of infiltrating cells, in th skin of mice treated with UVB alone ..
  7. Invasive Behavior of Tumor Cells Producing Collagenase-1
    Constance E Brinckerhoff; Fiscal Year: 2013
    ..This is accomplished by the collagenases, of which MMP-1 is the most ubiquitously expressed...
  8. Defining Common Molecular Parameters For Onset and Progression of Osteoarthritis
    Roland Wolkowicz; Fiscal Year: 2010
    ..Moreover, interrogation against available human OA datasets will have clinically relevant impact in the context of the "whole joint" and uncover new therapeutic targets. ..
  9. Effects of mechanical forces on lung injury and repair
    Bela Suki; Fiscal Year: 2010
    ..The research proposed here involves investigating how cells and connective tissue stretch in the lung during breathing and may open a new direction in understanding the disease with high potential to develop new treatments. ..
  10. Targeting stromal reaction in pancreatic cancer to overcome chemo-resistance
    Hidayatullah G Munshi; Fiscal Year: 2013
    ..abstract_text> ..
  11. A Protective Role for Fibroblast Activation Protein (FAP) in Pulmonary Fibrosis
    Ming Hui Fan; Fiscal Year: 2013
    ..Dr. Fan's project will determine the mechanism(s) by which FAP protects against the development of pulmonary fibrosis after lung injury. ..
  12. New probes for matrix metalloproteinase 13
    Gregg B Fields; Fiscal Year: 2013
    ..In addition, a unique mode of MMP inhibition will be identified. ..
  13. ESE 1 a novel transcriptional regulator of cartilage remodeling
    MARY GOLDRING; Fiscal Year: 2009
    ..Project Description ..
  14. Matrix remodeling in tumor growth and metastasis in a murine breast cancer model
    Lisa L Chang; Fiscal Year: 2011
    ..Further, our studies may lead to an early breast cancer diagnostic biomarker that could be used in the clinic to save time, resources and most importantly, life. ! ..
  15. Redox-Control of MMP-1 and Senescence
    JUAN ANDRES MELENDEZ; Fiscal Year: 2012
  16. Neutrophil Collagenase in Sepsis and Ventilator Induced Lung Injury
    A Murat Kaynar; Fiscal Year: 2013
    ..In this proposal, we will study a key molecule, namely matrix metalloproteinase (MMP)-8 in mouse models of sepsis, acute lung injury and the possible injury inflicted by the use of mechanical ventilation. ..
  17. Role of Prostaglandin Receptors in Stroke
    SYLVAIN DORE; Fiscal Year: 2013
    ..Such information is required to develop and test receptor agonist/antagonist interventions for potential use in clinical trials for hemorrhagic stroke. ..
  18. Non-Enzymatic Cryogenic Isolation of Therapeutic Cells
    MICHAEL JOHN TAYLOR; Fiscal Year: 2012
  19. Molecular Determinants of Arterial Remodeling in Atherogenesis
    Peter Libby; Fiscal Year: 2013
    ..g., collagenases and elastases...
  20. Regulation of Limbal Epithelial Stem Cells by the Native Niche
    Scheffer C G Tseng; Fiscal Year: 2013
    ..Such an approach may one day help realize the considerable promise held by adult SCs in treating a number of diseases in the body. ..
  21. MMP14-dependent regulation of adipose tissue remodeling and function (R01)
    Tae Hwa Chun; Fiscal Year: 2013
    ..The knowledge will help us understand the pathogenesis of insulin resistance and obesity-related metabolic diseases from the perspective of peri-adipocyte collagen turnover. ..
    Keith Brew; Fiscal Year: 2013
    ..ADAMTS-4 and -5, and screen human antibody phage display libraries for "exosite inhibitors" of aggrecanases and collagenases. The prototype exosite inhibitors will be further refined by mutation...
  23. PGE2 EP1 and EP3 receptors as therapeutic targets in intracerebral hemorrhage
    Jian Wang; Fiscal Year: 2013
    ..This proof-of-concept information is required to plan more detailed preclinical assessment of selective EP1 and EP3 receptor antagonists in ICH. ..
  24. Molecular Mechanisms by which TNFalpha Modulates Fibrosis
    David A Brenner; Fiscal Year: 2013
    ..This proposal uses the wound healing model of hepatic fibrosis to assess how TNF? modulates wound healing through different signal transduction pathways in the whole organism and in specific hepatic cell populations. ..
  25. Protective Effect of Hemin Preconditioning after Intracerebral Hemorrhage
    Raymond F Regan; Fiscal Year: 2013
    ..It is hoped that the results of this project will define the benefits of systemic hemin after ICH, and rapidly lead to clinical trials for a disease process that currently has few therapeutic options. ..
  26. Biophysical Regulation of Breast Differentiation
    Patricia J Keely; Fiscal Year: 2013
    ..These experiments are designed to understand the underlying molecular mechanisms by which the dense extracellular matrix regulates breast cell behavior and invasion and progression, and could suggest future targets for therapy. ..
  27. Structural Studies of Triple-Helical Proteins
    BARBARA M BRODSKY; Fiscal Year: 2013
  28. Vascularization of polymeric tissue beds
    Horst A von Recum; Fiscal Year: 2010
    ..Successful completion of the research will generate fundamental insights into the requirements needed for developing vascular networks in engineered tissue constructs and facilitate translation into clinical applications. ..
  29. Reasons for poor durability of resin-dentin bonds
    David H Pashley; Fiscal Year: 2013
    ..We have discovered a number of QAC-containing monomers that inhibit collagenases. If we can incorporate them into adhesive resins, we may be able to permanently inhibit dentin MMPs that are ..
  30. Matrix Metalloproteinase-8 in Obliterative Bronchiolitis Post Lung Transplant
    Meera Subramaniam; Fiscal Year: 2010
    ..This work will clarify the role of MMP-8 in the pathophysiology of OB and may suggest new insights into MMP-8-blockade as a novel therapeutic target to ameliorate OB. ..
  31. Role of C/EBPepsilon in myeloid differentiation
    Stephanie Halene; Fiscal Year: 2010
    ..Verified important targets will be transduced into C/EBPe-/- marrow progenitors and transplanted back into C/EBPe-/- mice to assess reversal of phenotype. ..
  32. Fibroblast Activation Protein-alpha Activated Anti-Stromal Prodrug Therpay for Ca
    Samuel R Denmeade; Fiscal Year: 2010
    ..e. "stroma") within cancers rather than the cancer cells themselves can be effective therapy. The FAP- activated prodrug, therefore, could represent a new targeted therapy for a variety of human cancers. ..
  33. Biomechanical Mechanisms of Artery Tortuosity
    Hai Chao Han; Fiscal Year: 2013
    ..Understanding the biomechanical mechanisms how tortuosity initiates and develop will reveal its role in vascular disease and lead to new understanding of vascular disease and set a basis for development of new treatment. ..
    MARY SHARON STACK; Fiscal Year: 2013
    ..Understanding this transition will enable novel means of targeting intraperitoneal therapies to appropriate multicellular populations. ..
  35. Plasmalemma permeability and necroptosis: New targets for intracerebral hemo
    Michael J Whalen; Fiscal Year: 2013
    ..Completion of these Aims will establish necrosis as central to ICH, and may establish VA64 and necrostatins as novel therapies for ICH. ..
  36. Post Transcriptional Control of hemorrhagic iron damage.
    Jack T Rogers; Fiscal Year: 2013
    ..apos; ..
  37. A fluorescent method to quantify neuronal injury after intracerebral hemorrhage
    Raymond F Regan; Fiscal Year: 2012
    ..It is hoped that the proposed studies will establish the validity of this rapid fluorescent method for quantifying neuronal injury after experimental ICH. ..
  38. Membrane-type 1 Matrix Metalloproteinase in COPD
    Jeffrey J Atkinson; Fiscal Year: 2012
    ..of COPD has been attributed to cigarette smoke induced-inflammation and elastase production, but recently collagenases have been implicated in the development of emphysema...
  39. Distinguishing adipose stromal vs. stem cells by serial transplantation
    Bruce A Bunnell; Fiscal Year: 2013
    ..In addition, the outcomes will have far-reaching implications at the translational/clinical level with respect to regenerative medical strategies, approaches, and best-practices. ..
  40. Neuroprotective effect of flavanol (-) epicatechin after intracerebral hemorrhage
    Jian Wang; Fiscal Year: 2013
    ..Ultimately, the data may help the general public and healthcare providers make informed decisions on whether (-)-epicatechin could be accepted as an adjunct treatment in patients with ICH. ..
  41. Smoke Induced Airway Injury in the Lung
    JEANINE M D'ARMIENTO; Fiscal Year: 2013
    ..Upon completion of this proposal our goal is to determine whether the collagenolytic enzymes are a therapeutic target in emphysema and whether this pathway is useful as a disease susceptibility marker. ! ..
  42. CellGuard-TD improves human tissue digestion and cell subculturing
    Robert G Van Buskirk; Fiscal Year: 2011
    ..This particular project is designed to develop a CellGuard supplement that will improve the isolation and cell therapy application of human pancreatic islets leading to improved methods for treating diabetes. ..
  43. Non-invasive Morphologic and Biochemical Assessment of Tendon with Novel UTE-MRI
    Eric Y Chang; Fiscal Year: 2013
  44. Mitophagic and anti-angiogenic mechanism of heart failure
    Suresh C Tyagi; Fiscal Year: 2013
    ..The positive outcome of this project will have therapeutic ramifications for chronic heart failure. ..
  45. Therapeutic for Intracerebral Hemorrhage
    Dale Christensen; Fiscal Year: 2009
  46. MMP1-PAR1 Signaling in Vascular Integrity During Sepsis
    Caitlin J Foley; Fiscal Year: 2013
    ..The collagenolytic and PAR1 agonist activity profile of mColA and other secreted mouse collagenases will be determined in vitro and plasma expression levels and activity of these collagenases during sepsis ..
  47. B-Lymphocyte Immunotherapy in Islet Transplantation
    Ali Naji; Fiscal Year: 2011
    ..Islet transplantation has the potential to provide precise and long term control of glucose metabolism, thus preventing secondary complications of the disease. ..
    L Windsor; Fiscal Year: 2001
    STEPHEN JOSEPH WEISS; Fiscal Year: 2012
    ..PUBLIC HEALTH RELEVANCE: These studies should provide new insights into the role of the MT-MMPs in normal and neoplastic cell populations and define their potential importance as targets for novel therapeutic interventions. ..
    Francis G Spinale; Fiscal Year: 2010
    ..This study will develop methods to measure and block these enzymes following a heart attack. These results will help develop new tests and treatments for patients suffering from heart failure after a heart attack. ..
  51. Tissue Dissociation Enzymes for Islets and Other Cells
    ROBERT CRANE MCCARTHY; Fiscal Year: 2010
    ..Specifically, three recombinant constructs of Clostridia histolyticum collagenases: intact Class 2 (C2intact), intact Class 1 with two collagen binding domains (C1intact), and degraded C1 with a ..
    James Schreiber; Fiscal Year: 1999
    ..This information will further our basic understanding of the regulation of collagenase activity and are critical for development of future therapies for the prevention of preterm labor. ..
    Tayebeh Pourmotabbed; Fiscal Year: 2001
    ..These studies will further our understanding on the action of this enzyme and may lead to new therapeutic approaches which would focus on protection of articular tissues from degradation in rheumatoid arthritis. ..
  54. Improved recombinant collagenase for tissue dissociation
    Robert McCarthy; Fiscal Year: 2007
    ..After confirming the resistance of the mutated collagenases to protease degradation, enzyme production will be scaled up, & the mutant enzymes purified and assessed ..
    Anthony Poole; Fiscal Year: 2003
    ..we seek to identify molecular damage to athe triple helix of type Ii collagen (ordinarily caused by collagenases) that is thought to occur in ageing and occurs in IA...
    JEANINE D ARMIENTO; Fiscal Year: 2002
    ..Interstitial collagen is highly resistant to degradation by all proteinases other than collagenases. Using the alpha-myosin heavy chain promoter we have created a transgenic mouse which constitutively expresses ..
  57. Tenascin in Chondrosarcoma Metastasis
    Sean Scully; Fiscal Year: 2004
    ..It is also plausible that lessons learned in the proposed studies of chondrosarcoma can be applicable to a wider spectrum of neoplastic processes. ..
    Peter Torzilli; Fiscal Year: 2005
    ..and the tight packing of the collagen molecules into fibrils, effectively restricts the accessibility of the collagenases and gelatinases to the cleavage site within the collagen molecule...
    Martin Schwartz; Fiscal Year: 1993
    ..Our data indicate that, although highly homologous, the five MMP have sufficiently different peptide substrate specificities to permit construction of potent inhibitors which are highly specific for each of the five enzymes...
    Sunil Kapila; Fiscal Year: 2004
    ..The Independent Scientist Award will be critical in achieving these goals. ..
    Thomas Curry; Fiscal Year: 1999
    Mahboob Rahman; Fiscal Year: 2002
    ..of increase cartilage matrix catabolism by regulating various matrix-degrading enzymes, including the collagenases. Collagenase-3 is specifically expressed in skeletal cells including chondrocytes and has been shown to have an ..
    Virginia Kraus; Fiscal Year: 2002
    ..The hallmark of early OA is cartilage matrix degradation. Collagenases are members of the matrix metalloproteinase (MMP) family which play a major role in this cartilage destruction...
    Martin Graham; Fiscal Year: 1999
    ..How do mast cell proteases degrade human intestinal smooth muscle cell procollagen to collagen, and do the propeptide products of this cleavage regulate procollagen expression in human intestinal smooth muscle cells? ..
  65. Complex Formation and Protease Specificity
    Dieter Bromme; Fiscal Year: 2006
    ..Unique among structurally related cathepsins and MMP collagenases, cathepsin K cleaves interstitial collagens at multiple intra-helical sites and similar to bacterial ..