papain

Summary

Summary: A proteolytic enzyme obtained from Carica papaya. It is also the name used for a purified mixture of papain and CHYMOPAPAIN that is used as a topical enzymatic debriding agent. EC 3.4.22.2.

Top Publications

  1. ncbi Carboxy terminal extended phytocystatins are bifunctional inhibitors of papain and legumain cysteine proteinases
    Manuel Martinez
    Laboratorio de Bioquimica y Biologia Molecular, Departamento de Biotecnologia, Centro de Biotecnología y Genómica de Plantas, Universidad Politecnica de Madrid, ETS Ingenieros Agronomos, Madrid, Spain
    FEBS Lett 581:2914-8. 2007
  2. ncbi Beet yellows closterovirus: complete genome structure and identification of a leader papain-like thiol protease
    A A Agranovsky
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Russia
    Virology 198:311-24. 1994
  3. ncbi Crystal structure of the wild-type human procathepsin B at 2.5 A resolution reveals the native active site of a papain-like cysteine protease zymogen
    M Podobnik
    Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, Jamova 39, Ljubljana, 1111, Slovenia
    J Mol Biol 271:774-88. 1997
  4. pmc The crystal structure of Pseudomonas avirulence protein AvrPphB: a papain-like fold with a distinct substrate-binding site
    Minfeng Zhu
    Department of Biological Chemistry, Medical School and Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 101:302-7. 2004
  5. ncbi Processing and subcellular localization of the leader papain-like proteinase of Beet yellows closterovirus
    Roman A Zinovkin
    Department of Virology and Belozersky Institute of Physico Chemical Biology, Moscow State University, 119899 Moscow, Russia
    J Gen Virol 84:2265-70. 2003
  6. ncbi Grass group I allergens (beta-expansins) are novel, papain-related proteinases
    K Grobe
    University of California, La Jolla, CA, USA
    Eur J Biochem 263:33-40. 1999
  7. pmc The papain-like protease of severe acute respiratory syndrome coronavirus has deubiquitinating activity
    Naina Barretto
    Department of Microbiology and Immunology, Loyola University Chicago, Stritch School of Medicine, 2160 South First Avenue, Bldg 105, Maywood, IL 60153, USA
    J Virol 79:15189-98. 2005
  8. ncbi Protein engineering of nitrile hydratase activity of papain: molecular dynamics study of a mutant and wild-type enzyme
    Swarnalatha Y Reddy
    Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California 93106, USA
    J Am Chem Soc 124:12979-90. 2002
  9. pmc The tomato yellow leaf curl virus (TYLCV) V2 protein interacts with the host papain-like cysteine protease CYP1
    Amalia Bar-Ziv
    Institute of Plant Sciences A R O, The Volcani Center, Bet Dagan, Israel
    Plant Signal Behav 7:983-9. 2012
  10. pmc Papain-like protease 1 from transmissible gastroenteritis virus: crystal structure and enzymatic activity toward viral and cellular substrates
    Justyna A Wojdyla
    EMBL Hamburg Outstation, c o DESY, Notkestrasse 85, D 22603 Hamburg, Germany
    J Virol 84:10063-73. 2010

Detail Information

Publications244 found, 100 shown here

  1. ncbi Carboxy terminal extended phytocystatins are bifunctional inhibitors of papain and legumain cysteine proteinases
    Manuel Martinez
    Laboratorio de Bioquimica y Biologia Molecular, Departamento de Biotecnologia, Centro de Biotecnología y Genómica de Plantas, Universidad Politecnica de Madrid, ETS Ingenieros Agronomos, Madrid, Spain
    FEBS Lett 581:2914-8. 2007
    ..Phytocystatins (PhyCys) have been described as plant inhibitors of papain-like cysteine proteinases...
  2. ncbi Beet yellows closterovirus: complete genome structure and identification of a leader papain-like thiol protease
    A A Agranovsky
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Russia
    Virology 198:311-24. 1994
    ..The N-terminal domain showed sequence similarity to the helper component papain-like protease of potyviruses...
  3. ncbi Crystal structure of the wild-type human procathepsin B at 2.5 A resolution reveals the native active site of a papain-like cysteine protease zymogen
    M Podobnik
    Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, Jamova 39, Ljubljana, 1111, Slovenia
    J Mol Biol 271:774-88. 1997
    ..The structure strongly suggests that processing of procathepsin B during its autoactivation is not unimolecular...
  4. pmc The crystal structure of Pseudomonas avirulence protein AvrPphB: a papain-like fold with a distinct substrate-binding site
    Minfeng Zhu
    Department of Biological Chemistry, Medical School and Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 101:302-7. 2004
    ..The core of this structure resembles the papain-like cysteine proteases...
  5. ncbi Processing and subcellular localization of the leader papain-like proteinase of Beet yellows closterovirus
    Roman A Zinovkin
    Department of Virology and Belozersky Institute of Physico Chemical Biology, Moscow State University, 119899 Moscow, Russia
    J Gen Virol 84:2265-70. 2003
    ORF 1a of Beet yellows closterovirus (BYV) encodes the domains of the papain-like proteinase (PCP), methyltransferase (MT) and RNA helicase...
  6. ncbi Grass group I allergens (beta-expansins) are novel, papain-related proteinases
    K Grobe
    University of California, La Jolla, CA, USA
    Eur J Biochem 263:33-40. 1999
    ..of Phl p 1 with three functionally important sequence motifs surrounding the active-site amino acids of the C1 (papain-like) family of cysteine proteinases...
  7. pmc The papain-like protease of severe acute respiratory syndrome coronavirus has deubiquitinating activity
    Naina Barretto
    Department of Microbiology and Immunology, Loyola University Chicago, Stritch School of Medicine, 2160 South First Avenue, Bldg 105, Maywood, IL 60153, USA
    J Virol 79:15189-98. 2005
    ..syndrome coronavirus (SARS-CoV) is mediated by replicase polyproteins that are processed by two viral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro)...
  8. ncbi Protein engineering of nitrile hydratase activity of papain: molecular dynamics study of a mutant and wild-type enzyme
    Swarnalatha Y Reddy
    Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California 93106, USA
    J Am Chem Soc 124:12979-90. 2002
    ..of hydrolysis of the nitrile (N-acetyl-phenylalanyl-2-amino-propionitrile, I) catalyzed by Gln19Glu mutant of papain has been studied by nanosecond molecular dynamics (MD) simulations...
  9. pmc The tomato yellow leaf curl virus (TYLCV) V2 protein interacts with the host papain-like cysteine protease CYP1
    Amalia Bar-Ziv
    Institute of Plant Sciences A R O, The Volcani Center, Bet Dagan, Israel
    Plant Signal Behav 7:983-9. 2012
    ..Specifically, we show that V2 recognizes and directly binds the tomato CYP1 protein, a member of the family of papain-like cysteine proteases which are involved in plant defense against diverse pathogens...
  10. pmc Papain-like protease 1 from transmissible gastroenteritis virus: crystal structure and enzymatic activity toward viral and cellular substrates
    Justyna A Wojdyla
    EMBL Hamburg Outstation, c o DESY, Notkestrasse 85, D 22603 Hamburg, Germany
    J Virol 84:10063-73. 2010
    ..encode two classes of cysteine proteases, which have narrow substrate specificities and either a chymotrypsin- or papain-like fold...
  11. ncbi Beta-lactone probes identify a papain-like peptide ligase in Arabidopsis thaliana
    Zheming Wang
    Plant Chemetics Lab, Max Planck Institute for Plant Breeding Research, Carl von Linne Weg 10, 50829 Cologne, Germany
    Nat Chem Biol 4:557-63. 2008
    ..and reverse genetics led to the discovery that this unusual modification is mediated by a single plant-specific, papain-like protease called RD21...
  12. doi Papain-like proteases of Staphylococcus aureus
    Tomasz Kantyka
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Adv Exp Med Biol 712:1-14. 2011
    ..aureus secretes several proteases of three catalytic classes-metallo, serine and papain-like cysteine proteases...
  13. doi Antioxidant and antiproliferative activities of loach ( Misgurnus anguillicaudatus ) peptides prepared by papain digestion
    Lijun You
    College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China
    J Agric Food Chem 59:7948-53. 2011
    Loach protein hydrolysates (LPH) prepared by papain digestion were fractionated into four fractions, LPH-I (MW > 10 kDa), LPH-II (MW = 5-10 kDa), LPH-III (MW = 3-5 kDa), LPH-IV (MW < 3 kDa), and the in vitro antioxidant and ..
  14. ncbi Inhibition mechanism of cathepsin L-specific inhibitors based on the crystal structure of papain-CLIK148 complex
    H Tsuge
    Institute for Health Sciences, Tokushima Bunri University, Yamashiro cho, Tokushima, 770 8514, Japan
    Biochem Biophys Res Commun 266:411-6. 1999
    b>Papain was used as an experimental model structure to understand the inhibition mechanism of newly developed specific inhibitors of cathepsin L, the papain superfamily...
  15. ncbi Thyroglobulin type-1 domains in equistatin inhibit both papain-like cysteine proteinases and cathepsin D
    B Lenarcic
    Department of Biochemistry and Molecular Biology, J Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia
    J Biol Chem 274:563-6. 1999
    Equistatin from sea anemone is a protein composed of three thyroglobulin-type 1 domains known to inhibit papain-like cysteine proteinases, papain, and cathepsins B and L...
  16. ncbi Papain-like cysteine proteases: key players at molecular battlefields employed by both plants and their invaders
    Takayuki Shindo
    Plant Chemetics Laboratory, Max Planck Institute for Plant Breeding Research, Carl von Linne Weg 10, 50829 Cologne, Germany
    Mol Plant Pathol 9:119-25. 2008
    b>Papain-like cysteine proteases (PLCPs) play crucial roles in plant-pathogen/pest interactions...
  17. pmc Structure of the foot-and-mouth disease virus leader protease: a papain-like fold adapted for self-processing and eIF4G recognition
    A Guarné
    Centre d Investigació i Desenvolupament CSIC, Jordi Girona Salgado 18 26, E 08034 Barcelona, Spain
    EMBO J 17:7469-79. 1998
    ..protease presented here comprises a globular catalytic domain reminiscent of that of cysteine proteases of the papain superfamily, and a flexible C-terminal extension found intruding into the substrate-binding site of an adjacent ..
  18. pmc Critical role of amino acid 23 in mediating activity and specificity of vinckepain-2, a papain-family cysteine protease of rodent malaria parasites
    Ajay Singh
    Department of Medicine, San Francisco General Hospital, San Francisco, CA 94143, USA
    Biochem J 368:273-81. 2002
    ..vinckei (vinckepain-2) and P. berghei (berghepain-2) were characterized. These genes encoded fairly typical papain-family proteases, but they contained an unusual substitution of Gly23 with Ala (papain numbering system)...
  19. doi Crystal structure of the parasite inhibitor chagasin in complex with papain allows identification of structural requirements for broad reactivity and specificity determinants for target proteases
    Izabela Redzynia
    Institute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Technical University of Lodz, Poland
    FEBS J 276:793-806. 2009
    A complex of chagasin, a protein inhibitor from Trypanosoma cruzi, and papain, a classic family C1 cysteine protease, has been crystallized. Kinetic studies revealed that inactivation of papain by chagasin is very fast (k(on) = 1...
  20. pmc Weeds, worms, and more. Papain's long-lost cousin, phytochelatin synthase
    Philip A Rea
    Plant Science Institute, Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Plant Physiol 136:2463-74. 2004
  21. ncbi Human cathepsin X: a novel cysteine protease of the papain family with a very short proregion and unique insertions
    D K Nägler
    Biotechnology Research Institute, National Research Council of Canada, Montreal, Que
    FEBS Lett 434:135-9. 1998
    A novel cDNA encoding a cysteine protease of the papain family named cathepsin X was obtained by PCR amplification from a human ovary cDNA library...
  22. ncbi Molecular cloning, expression, and functional characterization of a cystatin from pineapple stem
    Douglas J H Shyu
    Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan
    Biosci Biotechnol Biochem 68:1681-9. 2004
    ..The fusion and non-fusion cystatins were separately purified to homogeneity via a His-tag or papain-coupling affinity column...
  23. ncbi Loosely packed papain prosegment displays inhibitory activity
    Luis H Gutiérrez-González
    Area de Biofisicoquímica, Departamento de Quimica, Universidad Autonoma Metropolitana Iztapalapa, Apartado Postal 55 534, 09340 Mexico City, Mexico
    Arch Biochem Biophys 446:151-60. 2006
    ..By using circular dichroism, UV-difference and fluorescence spectroscopies, we studied the thermal unfolding of papain prosegment. The transition seems to be two-state and reversible, with an unfolded state prone to aggregation...
  24. ncbi Identification of internal autoproteolytic cleavage sites within the prosegments of recombinant procathepsin B and procathepsin S. Contribution of a plausible unimolecular autoproteolytic event for the processing of zymogens belonging to the papain family
    O Quraishi
    Protein Engineering Network of Centres of Excellence and Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada
    J Biol Chem 276:8118-24. 2001
    The steps involved in the maturation of proenzymes belonging to the papain family of cysteine proteases have been difficult to characterize...
  25. ncbi Papain gel: a new chemo-mechanical caries removal agent
    Sandra Kalil Bussadori
    FUNDECTO USP, Universidade de Sao Paulo USP, Brazil
    J Clin Pediatr Dent 30:115-9. 2005
    ..Among different kinds of chemo-mechanical caries removal systems, Papacarie--a papain gel--was found to be easy to manipulate, simple and cheap, as well as effective in removing infected tissues.
  26. doi Two routes for production and purification of Fab fragments in biopharmaceutical discovery research: Papain digestion of mAb and transient expression in mammalian cells
    Yonghong Zhao
    Centocor R and D Inc, 145 King of Prussia Road, Radnor, PA 19087, USA
    Protein Expr Purif 67:182-9. 2009
    ..One route utilizes papain digestion of an intact monoclonal antibody for Fab fragment production...
  27. doi Papain-catalyzed peptide bond formation: enzyme-specific activation with guanidinophenyl esters
    Roseri J A C de Beer
    Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands
    Chembiochem 12:2201-7. 2011
    ..for the serine proteases trypsin and chymotrypsin, has now been applied for the first time in combination with papain, a cheap and commercially available cysteine protease...
  28. doi Mechanism for stabilization of the molten globule state of papain by sodium n-alkyl sulfates: spectroscopic and calorimetric approaches
    J Chamani
    Department of Biology, Faculty of Science, Islamic Azad University Mashhad Branch, Mashhad, Iran
    J Colloid Interface Sci 322:119-27. 2008
    b>Papain exists in a molten globule (MG) state at pH 2 and in this state protein tends to aggregate in the presence of lower concentrations of guanidine hydrochloride (GuHCl)...
  29. ncbi Effects of a novel pathogenesis-related class 10 (PR-10) protein from Crotalaria pallida Roots with papain inhibitory activity against root-knot nematode Meloidogyne incognita
    Lúcia Betânia da S Andrade
    Centro de Ciencias Agrarias e Biologicas, Universidade Estadual Vale do Acarau UVA, Sobral, Ceara CE, Brazil
    J Agric Food Chem 58:4145-52. 2010
    A novel pathogenesis-related class 10 (PR-10) protein with papain inhibitory activity, named CpPRI, was purified from Crotalaria pallida roots by ammonium sulfate precipitation followed by three reverse-phase high-performance liquid ..
  30. doi A distinct subfamily of papain-like cystein proteinases regulated by senescence and stresses in Glycine max
    Belén Esteban-García
    Grupo de Biotecnología de Productos Naturales BIO 279, Facultad de Ciencias Experimentales, Universidad de Almeria, 04120 Almeria, Spain
    J Plant Physiol 167:1101-8. 2010
    GMCP3 encodes a cystein proteinase of Glycine max belonging to the papain-like family (C1A in MEROPS database) that was previously found to be involved in the mobilization of protein reserves during seed germination...
  31. pmc Purification and in situ immobilization of papain with aqueous two-phase system
    Mingliang Li
    State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, China
    PLoS ONE 5:e15168. 2010
    b>Papain was purified from spray-dried Carica papaya latex using aqueous two-phase system (ATPS). Then it was recovered from PEG phase by in situ immobilization or preparing cross-linked enzyme aggregates (CLEAs)...
  32. doi Stain removal efficacy of a novel dentifrice containing papain and Bromelain extracts--an in vitro study
    P Kalyana
    Department of Community Dentistry, Manipal College of Dental Sciences, Manipal, India
    Int J Dent Hyg 9:229-33. 2011
    ..To assess the in vitro stain removal efficacy of a novel commercially available dentifrice...
  33. ncbi Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG
    Johnson Agniswamy
    Structural Immunology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12441 Parklawn Drive, Rockville, Maryland 20852, USA
    Structure 14:225-35. 2006
    ..Despite the lack of sequence homology, Mac-1 adopts the canonical papain fold...
  34. ncbi Papain digestion of different mouse IgG subclasses as studied by electrospray mass spectrometry
    M Adamczyk
    Department of Chemistry 9NM, Abbott Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, Abbott Park, IL 60064 6016, USA
    J Immunol Methods 237:95-104. 2000
    ..On-line liquid chromatography-electrospray ionization mass spectrometry (LC/ESI-MS) has been utilized to monitor the papain digestion of mouse monoclonal antibodies (mAb) of different subclasses (IgG1, IgG2a, IgG2b, and IgG3)...
  35. pmc Crystal structure of tarocystatin-papain complex: implications for the inhibition property of group-2 phytocystatins
    Ming Hung Chu
    Department of Life Science and Institute of Plant Biology, National Taiwan University, No 1, Sec 4, Roosevelt Road, Taipei, 10617, Taiwan, ROC
    Planta 234:243-54. 2011
    ..The structure of the tarocystatin-papain complex and the domain interaction between NtD and CtE in tarocystatin have not been determined...
  36. doi Enzyme catalytic promiscuity: The papain-catalyzed Knoevenagel reaction
    Wen Hu
    School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China
    Biochimie 94:656-61. 2012
    b>Papain as a sustainable and inexpensive biocatalyst was used for the first time to catalyze the Knoevenagel reactions in DMSO/water...
  37. doi Papain-specific activating esters in aqueous dipeptide synthesis
    Roseri J A C de Beer
    Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands
    Chembiochem 13:1319-26. 2012
    ..Finally, selected ODmap- and OBn-activated amino acids were applied in the synthesis of two biologically active dipeptides on preparative scales...
  38. ncbi Physiologic responses of sheep to two different methods of papain exposure
    Andrew Hoffman
    Department of Clinical Sciences, Tufts University School of Veterinary Medicine, 200 Westboro Rd, North Grafton, MA 01536, USA
    Inhal Toxicol 15:761-80. 2003
    ..Emphysema was produced by two different techniques of papain exposure: (1) aerosol (75 IU/kg) given weekly for 4 treatments (HM) or (2) aerosol (75 IU/kg) weekly for 3 ..
  39. pmc A Phytophthora infestans cystatin-like protein targets a novel tomato papain-like apoplastic protease
    Miaoying Tian
    Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, Ohio 44691, USA
    Plant Physiol 143:364-77. 2007
    ..Biochemical functional analyses revealed that EPIC2B interacts with and inhibits a novel papain-like extracellular cysteine protease, termed Phytophthora Inhibited Protease 1 (PIP1)...
  40. doi Immobilization of modified papain with anhydride groups on activated cotton fabric
    Yong Xue
    College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, China
    Appl Biochem Biotechnol 160:109-21. 2010
    b>Papain (EC 3.4.22.2) has been chemically modified using two novel reagents including different anhydrides of 1,2,4-benzenetricarboxylic and pyromellitic acids...
  41. pmc Proteolytic processing and deubiquitinating activity of papain-like proteases of human coronavirus NL63
    Zhongbin Chen
    Department of Microbiology and Immunology, Loyola University Medical Center, 2160 South First Avenue, Bldg 105, Rm 3929, Maywood, IL 60153, USA
    J Virol 81:6007-18. 2007
    ..Here, we identify HCoV-NL63 replicase gene products and characterize two viral papain-like proteases (PLPs), PLP1 and PLP2, which process the viral replicase polyprotein...
  42. ncbi Glycosylation in the Fc domain of IgG increases resistance to proteolytic cleavage by papain
    T Shantha Raju
    Discovery Research, Centocor R and D Inc, 145 King of Prussia Road, Radnor, PA 19087, USA
    Biochem Biophys Res Commun 341:797-803. 2006
    ..fragments of IgGs include Fc, Fab, and F(ab')2 fragments, all of which can be made using the sulfhydryl protease papain, although prolonged digestion times and/or excessive amounts of papain typically result in further cleavage of the ..
  43. pmc Deubiquitinase function of arterivirus papain-like protease 2 suppresses the innate immune response in infected host cells
    Puck B van Kasteren
    Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, 2333 ZA, Leiden, The Netherlands
    Proc Natl Acad Sci U S A 110:E838-47. 2013
    ..are economically important positive-stranded RNA viruses that encode an ovarian tumor (OTU) domain DUB known as papain-like protease 2 (PLP2)...
  44. ncbi A new multi-domain member of the cystatin superfamily expressed by Fasciola hepatica
    Eric Khaznadji
    INRA, UR86 Bio Agresseurs, Santé et Environnement 37 380 Nouzilly, France
    Int J Parasitol 35:1115-25. 2005
    ..This protein was a potent inhibitor of the papain and of the major cysteine protease of F. hepatica, the cathepsin L1.
  45. ncbi Affinity selection to papain yields potent peptide inhibitors of cathepsins L, B, H, and K
    Tomaz Bratkovic
    Faculty of Pharmacy, Department of Pharmaceutical Biology, University of Ljubljana, Askerceva 7, SI 1000 Ljubljana, Slovenia
    Biochem Biophys Res Commun 332:897-903. 2005
    ..In the presented work, papain was chosen as a model cysteine protease and panning protocol was optimized for selection of papain-binding phage-..
  46. ncbi Optimal conditions for the papain digestion of polyclonal ovine IgG for the production of bio-therapeutic Fab fragments
    Chrissie Cresswell
    Process Development Group, Protherics U K Ltd, Blaenwaun, Ffostrasol, Llandysul, Wales SA44 5JT, U K
    Biotechnol Appl Biochem 42:163-8. 2005
    In the present paper, we describe a rapid method for the determination of optimum conditions for papain digestion of polyclonal ovine IgG (purified by Na(2)SO(4) precipitation) for the production of bio-therapeutic Fabs (antigen-binding ..
  47. ncbi Characterization of a partially folded intermediate of papain induced by fluorinated alcohols at low pH
    Aabgeena Naeem
    Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India
    Arch Biochem Biophys 432:79-87. 2004
    ..1,1,1,3,3,3-hexafluoroisopropanol (HFIP) and 2,2,2-trifluoroethanol (TFE) on the structure of acid-unfolded papain (EC. 3.4.22...
  48. doi Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria
    Raka Ghosh
    Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata, India
    FEBS J 275:421-34. 2008
    ..Ervatamin-A, ervatamin-B and ervatamin-C are three such papain-like cysteine proteases found in the latex of the tropical plant Ervatamia coronaria, and are known not only for ..
  49. ncbi Two Kunitz-type inhibitors with activity against trypsin and papain from Pithecellobium dumosum seeds: purification, characterization, and activity towards pest insect digestive enzyme
    A S Oliveira
    Laboratório de Química eFunção de Proteínas, Departamento de Bioquimica, Centro de Biociências UFRN, Natal RN, Brazil
    Protein Pept Lett 16:1526-32. 2009
    ..20 x 10(-8) and 2.88 x 10(-8) M, and also moderately inhibited papain activity, a cysteine proteinase. PdKI-3.1 and PdKI-3...
  50. ncbi Effect of a papain-based gel for chemomechanical caries removal on dentin shear bond strength
    Michelle Cristina Lopes
    Araras Dental School, Herminio Ometto University Center UNIARARAS, Araras, Sao Paulo, Brazil
    J Dent Child (Chic) 74:93-7. 2007
    The aim of this study was to assess the shear bond strength of an adhesive restorative system on sound and demineralized dentin after the use of a papain-based agent.
  51. ncbi Nontraditional wound care: A review of the evidence for the use of sugar, papaya/papain, and fatty acids
    Barbara Pieper
    College of Nursing, Wayne State University, Detroit, Michigan, USA
    J Wound Ostomy Continence Nurs 30:175-83. 2003
    ..The purpose of this article is to review the evidence about 3 commonly used wound products, namely, sugar, papaya/papain, and essential fatty acids...
  52. pmc Interaction of recombinant human cystatin C with the cysteine proteinases papain and actinidin
    P Lindahl
    Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala Biomedical Center
    Biochem J 281:49-55. 1992
    The interaction between recombinant human cystatin C and the cysteine proteinases papain and actinidin was studied by spectroscopic, kinetic and equilibrium methods. The absorption, near-u.v.c.d...
  53. ncbi Differential effect toward inhibition of papain and cathepsin C by recombinant human salivary cystatin SN and its variants produced by a baculovirus system
    C C Tseng
    Divisions of Basic Sciences and of Restorative and Prosthodontic Sciences, Room 1027 S, College of Dentistry, New York University, 345 East 24th Street, New York, New York 10010 4086, USA
    Arch Biochem Biophys 380:133-40. 2000
    ..The purified proteins were examined for papain- and cathepsin C-inhibition...
  54. ncbi An amplified assay for thiols based on reactivation of papain
    R Singh
    ImmunoGen, Inc, Cambridge, Massachusetts 02139
    Anal Biochem 213:49-56. 1993
    ..assay has been developed for thiol (sulfhydryl) groups using an inactive disulfide derivative of papain (papain-S-SCH3)...
  55. ncbi The arterivirus Nsp2 protease. An unusual cysteine protease with primary structure similarities to both papain-like and chymotrypsin-like proteases
    E J Snijder
    Department of Virology, Faculty of Medicine, Leiden University, The Netherlands
    J Biol Chem 270:16671-6. 1995
    ..A papain-like Cys protease in Nsp1 and a chymotrypsin-like Ser protease in Nsp4 are involved in this process...
  56. ncbi Morphogenesis of rat experimental pulmonary emphysema induced by intratracheally administered papain: changes in elastic fibres
    L M Pastor
    Department of Cell Biology, Medical School, Aging Institute, University of Murcia, Murcia, Spain
    Histol Histopathol 21:1309-19. 2006
    ..this paper, the morphogenesis of emphysema and alterations of the elastic fibres produced by the instillation of papain are described by light and electron microscopy...
  57. pmc Homology of amino acid sequences of rat liver cathepsins B and H with that of papain
    K Takio
    Proc Natl Acad Sci U S A 80:3666-70. 1983
    ..thiol endopeptidases, cathepsins B and H, are presented and compared with that of the plant thiol protease papain. The 252-residue sequence of cathepsin B and the 220-residue sequence of cathepsin H were determined largely by ..
  58. ncbi Purification and characterization of a trypsin-papain inhibitor from Pithecelobium dumosum seeds and its in vitro effects towards digestive enzymes from insect pests
    Adeliana S Oliveira
    Departamento Bioquimica, Laboratório de Química e Função de Proteínas, Centro de Biociencias, Campus UFRN, Av Salgado Filho s n, 59072 970 Natal, RN, Brazil
    Plant Physiol Biochem 45:858-65. 2007
    A novel trypsin-papain inhibitor, named PdKI-2, was purified from the seeds of Pithecelobium dumosum seeds by TCA precipitation, Trypsin-Sepharose chromatography and reversed-phase HPLC. PdKI-2 had an M(r) of 18...
  59. ncbi Chemo-mechanical removal of caries in an adolescent patient using a papain gel: case report
    S K Bussadori
    Nove de Julho University, Sao Paulo, Brazil
    J Clin Pediatr Dent 32:177-80. 2008
    ..Papacarie is a papain gel composed basically of papain, chloramines, and toluidine blue...
  60. ncbi Removal of mercury from its aqueous solution using charcoal-immobilized papain (CIP)
    Susmita Dutta
    Department of Chemical Engineering, National Institute of Technology, Durgapur, Durgapur 713209, India
    J Hazard Mater 172:888-96. 2009
    In the present work mercury has been eradicated from its aqueous solution using papain, immobilized on activated charcoal by physical adsorption method...
  61. ncbi Structural basis for the specificity and catalysis of human Atg4B responsible for mammalian autophagy
    Kenji Sugawara
    Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, N 12, W 6, Kita ku, Sapporo 060 0812, Japan
    J Biol Chem 280:40058-65. 2005
    ..Despite no obvious sequence homology with known proteases, the structure of HsAtg4B shows a classical papain-like fold. In addition to the papain fold region, HsAtg4B has a small alpha/beta-fold domain...
  62. doi Impacts of papain and neuraminidase enzyme treatment on electrohydrodynamics and IgG-mediated agglutination of type A red blood cells
    Atsushi Hyono
    Faculty of Pharmaceutical Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Langmuir 25:10873-85. 2009
    ..These properties significantly depend on the concentration of proteolytic papain enzyme and protease-free neuraminidase enzyme that the cells are exposed to...
  63. ncbi Physicochemical characterization of papain entrapped in ionotropically cross-linked kappa-carrageenan gel beads for stability improvement using Doehlert shell design
    Mayur G Sankalia
    Pharmacy Department, Centre of Relevance and Excellence in Novel Drug Delivery Systems, G H Patel building, Donor s Plaza, The M S University of Baroda, Vadodara 390 002, India
    J Pharm Sci 95:1994-2013. 2006
    This work examines the influence of various process parameters on papain entrapped in cross-linked kappa-carrageenan beads for improvement of its stability...
  64. ncbi Microtensile bond strength of etch-and-rinse and self-etch adhesive systems to demineralized dentin after the use of a papain-based chemomechanical method
    Renato José Gianini
    Faculty of Dentistry and Dental Research Center São Leopoldo Mandic, Campinas, Sao Paulo, Brazil
    Am J Dent 23:23-8. 2010
    To evaluate the in vitro microtensile bond strength (microTBS) of etch-and-rinse and self-etch adhesive systems to demineralized dentin after the use of a papain-based chemomechanical method.
  65. pmc Differences in the chemical and catalytic characteristics of two crystallographically 'identical' enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent substrate catalysis kinetics and reactivity probe studies t
    E Salih
    Department of Biochemistry, St Bartholomew s Hospital Medical College, University of London, U K
    Biochem J 247:181-93. 1987
    The characteristics of actinidin (EC 3.4.22.14) and papain (EC 3.4.22...
  66. ncbi AtCYS1, a cystatin from Arabidopsis thaliana, suppresses hypersensitive cell death
    Beatrice Belenghi
    Dipartimento Scientifico e Tecnologico, Universita degli Studi di Verona, Verona, Italy
    Eur J Biochem 270:2593-604. 2003
    ..AtCYS1 inhibits the catalytic activity of papain (Kd = 4.0 x 10-2 micro m, at pH 7.0 and 25 degrees C), generally taken as a molecular model of cysteine proteases...
  67. ncbi Human and parasitic papain-like cysteine proteases: their role in physiology and pathology and recent developments in inhibitor design
    Fabien Lecaille
    Mount Sinai School of Medicine, Department of Human Genetics, Fifth Avenue at 100th Street, New York, New York 10029, USA
    Chem Rev 102:4459-88. 2002
  68. pmc A bacterial type III effector family uses the papain-like hydrolytic activity to arrest the host cell cycle
    Qing Yao
    National Institute of Biological Sciences, Beijing 102206, China
    Proc Natl Acad Sci U S A 106:3716-21. 2009
    ..Structure of one of the family members, Cif homolog in Burkholderia pseudomallei (CHBP), reveals a papain-like fold and a conserved Cys-His-Gln catalytic triad despite the lack of primary sequence identity...
  69. pmc High-resolution complex of papain with remnants of a cysteine protease inhibitor derived from Trypanosoma brucei
    Magnus S Alphey
    Division of Biological Chemistry and Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:504-8. 2006
    Attempts to cocrystallize the cysteine protease papain derived from the latex of Carica papaya with an inhibitor of cysteine proteases (ICP) from Trypanosoma brucei were unsuccessful...
  70. ncbi A multicystatin is induced by drought-stress in cowpea (Vigna unguiculata (L.) Walp.) leaves
    Ndeye Ndack Diop
    Laboratoire d Ecophysiologie Moléculaire, UMR IRD 137, FST, Universite Paris 12 Val de Marne, 61 Avenue du General de Gaulle, 94 010 Créteil cedex, France
    FEBS Lett 577:545-50. 2004
    Cystatins are protein inhibitors of cystein proteinases belonging to the papain family. In cowpea, cystatin-like polypeptides and a cDNA have been identified from seeds and metabolic functions have been attributed to them...
  71. doi PLP2, a potent deubiquitinase from murine hepatitis virus, strongly inhibits cellular type I interferon production
    Dahai Zheng
    Center for Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, 15 Da Tun Road, Chaoyang District, Beijing 100101, China
    Cell Res 18:1105-13. 2008
    ..Here, we provide evidence that Papain-like protease domain 2 (PLP2), a catalytic domain of the nonstructural protein 3 (nsp3) of MHV-A59, can bind to ..
  72. pmc The papain-like protease from the severe acute respiratory syndrome coronavirus is a deubiquitinating enzyme
    Holger A Lindner
    Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Quebec, Canada H4P 2R2
    J Virol 79:15199-208. 2005
    The severe acute respiratory syndrome coronavirus papain-like protease (SARS-CoV PLpro) is involved in the processing of the viral polyprotein and, thereby, contributes to the biogenesis of the virus replication complex...
  73. ncbi Light-induced inhibition of papain by a {Mn-NO}6 nitrosyl: identification of papain-SNO adduct by mass spectrometry
    Raman K Afshar
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    J Inorg Biochem 99:1458-64. 2005
    Modification of Cys25 at the active site of the cysteine protease papain by S-nitrosylation inhibits its hydrolytic ability...
  74. ncbi Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment
    Ai Saiga
    Graduate School of Biosphere Science, Hiroshima University, Higashi Hiroshima, 739 8528, Japan
    J Agric Food Chem 51:3661-7. 2003
    Hydrolysates obtained from porcine myofibrillar proteins by protease treatment (papain or actinase E) exhibited high antioxidant activity in a linolenic acid peroxidation system induced by Fe(2+)...
  75. ncbi Design, synthesis, and evaluation of in vivo potency and selectivity of epoxysuccinyl-based inhibitors of papain-family cysteine proteases
    Amir Masoud Sadaghiani
    Department of Pathology, Stanford School of Medicine, Stanford, CA 94305, USA
    Chem Biol 14:499-511. 2007
    The papain-family cathepsins are cysteine proteases that are emerging as promising therapeutic targets for a number of human disease conditions ranging from osteoporosis to cancer...
  76. ncbi Deubiquitinating activity of the SARS-CoV papain-like protease
    Naina Barretto
    Loyola University Chicago Stritch School of Medicine, Maywood, IL, USA
    Adv Exp Med Biol 581:37-41. 2006
  77. pmc Characterization of chito-oligosaccharides prepared by chitosanolysis with the aid of papain and Pronase, and their bactericidal action against Bacillus cereus and Escherichia coli
    Acharya B Vishu Kumar
    Department of Biochemistry and Nutrition, Central Food Technological Research Institute CFTRI, Mysore 570020, India
    Biochem J 391:167-75. 2005
    b>Papain (from papaya latex; EC 3.4.22.2) and Pronase (from Streptomyces griseus; EC 3.4.24.31) caused optimum depolymerization of chitosan at pH 3...
  78. pmc Hypovirus papain-like protease p29 functions in trans to enhance viral double-stranded RNA accumulation and vertical transmission
    Nobuhiro Suzuki
    Agrivirology Laboratory, Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710 0046, Japan
    J Virol 77:11697-707. 2003
    ..The papain-like protease, p29, and the highly basic protein, p40, derived, respectively, from the N-terminal and C-terminal ..
  79. pmc Replication of murine hepatitis virus is regulated by papain-like proteinase 1 processing of nonstructural proteins 1, 2, and 3
    Rachel L Graham
    Department of Pediatrics, Vanderbilt University Medical Center, D6217 MCN, 1161 21st Ave S, Nashville, TN 37232 2581, USA
    J Virol 80:11610-20. 2006
    ..In murine hepatitis virus (MHV), nsps 1, 2, and 3 are processed by two papain-like proteinase activities within nsp3 (PLP1 and PLP2) to yield nsp1, an nsp2-3 intermediate, and mature nsp2 and ..
  80. ncbi Enzymic, phylogenetic, and structural characterization of the unusual papain-like protease domain of Plasmodium falciparum SERA5
    Anthony N Hodder
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, 1G Royal Parade, Parkville, Victoria 3050, Australia
    J Biol Chem 278:48169-77. 2003
    ..All SERA proteins possess a central domain that has homology to papain except in the case of SERA5 (and some other SERAs), where the active site cysteine has been replaced with a serine...
  81. ncbi Inhibition of papain-like cysteine proteases and legumain by caspase-specific inhibitors: when reaction mechanism is more important than specificity
    J Rozman-Pungercar
    Department of Biochemistry and Molecular Biology, J Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    Cell Death Differ 10:881-8. 2003
    ..The in vitro studies included cathepsins B, H, L, S, K, F, V, X and C, papain and legumain...
  82. ncbi Selective disruption of lysosomes in HeLa cells triggers apoptosis mediated by cleavage of Bid by multiple papain-like lysosomal cathepsins
    Tina Cirman
    Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia
    J Biol Chem 279:3578-87. 2004
    ..to the cytosol, could be prevented by 15 microM L-trans-epoxysuccinyl(OEt)-Leu-3-methylbutylamide, an inhibitor of papain-like cysteine proteases...
  83. ncbi Electrostatic properties in the catalytic site of papain: A possible regulatory mechanism for the reactivity of the ion pair
    Laurent E Dardenne
    Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro UFRJ, CCS, Bloco G, Ilha do Fundao, 21949 900 Rio de Janeiro, RJ, Brazil
    Proteins 52:236-53. 2003
    We present an analysis of the electrostatic properties in the catalytic site of papain (EC 3.4.22...
  84. pmc Severe acute respiratory syndrome coronavirus papain-like protease: structure of a viral deubiquitinating enzyme
    Kiira Ratia
    Center for Pharmaceutical Biotechnology and Department of Medicinal Chemistry and Pharmacognosy, University of Illinois, Chicago, IL 60607, USA
    Proc Natl Acad Sci U S A 103:5717-22. 2006
    ..of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro)...
  85. pmc Mutagenic definition of a papain-like catalytic triad, sufficiency of the N-terminal domain for single-site core catalytic enzyme acylation, and C-terminal domain for augmentative metal activation of a eukaryotic phytochelatin synthase
    Nataliya D Romanyuk
    Plant Science Institute, Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Plant Physiol 141:858-69. 2006
    ..been determined that the N-terminal half of AtPCS1 and its equivalents from other sources have the hallmarks of a papain-like, Clan CA Cys protease...
  86. ncbi Production and activation of recombinant papain-like cysteine proteases
    Dieter Bromme
    Mount Sinai School of Medicine, Department of Human Genetics, New York, NY 10029 6574, USA
    Methods 32:199-206. 2004
    b>Papain-like cysteine proteases are the most numerous family of the cysteine protease class. They are expressed throughout the animal and plant kingdoms as well as in viruses and bacteria...
  87. ncbi Domain organization and movements in heavy metal ion pumps: papain digestion of CopA, a Cu+-transporting ATPase
    Yuta Hatori
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan
    J Biol Chem 282:25213-21. 2007
    ..from Thermotoga maritima was cloned, overexpressed, and purified, and then subjected to limited proteolysis using papain. Stable analogs of intermediate states were generated using AMPPCP as a nonhydrolyzable ATP analog and AlFx as a ..
  88. ncbi The novel serpin endopin 2 demonstrates cross-class inhibition of papain and elastase: localization of endopin 2 to regulated secretory vesicles of neuroendocrine chromaffin cells
    Shin Rong Hwang
    Buck Institute for Age Research, Novato, California 94945, USA
    Biochemistry 41:10397-405. 2002
    ..serpin that displays cross-class inhibition of cysteine and serine proteases, indicated by effective inhibition of papain and elastase, respectively...
  89. ncbi Antithrombotic papain-hydrolyzed peptides isolated from pork meat
    Muneshige Shimizu
    Research and Development Center, Nippon Meat Packers, Inc, Tsukuba, Ibaraki, Japan
    Thromb Res 123:753-7. 2009
    ..Platelets play important role in these arterial diseases. Suppression of platelet activity by materials may decrease incidence of the disease. The present study aimed to examine the peptides from pork meat with antithrombotic activity...
  90. doi Optimization of adsorption conditions of papain on dye affinity membrane using response surface methodology
    Sai Nan Su
    College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, No 2999 North Renmin Road, Shanghai 201620, China
    Bioresour Technol 100:2336-40. 2009
    The adsorption of papain on Reactive Blue 4 dye-ligand affinity membrane was investigated in a batch system...
  91. ncbi Structural and biochemical features distinguish the foot-and-mouth disease virus leader proteinase from other papain-like enzymes
    A Guarné
    Institut de Biologia Molecular de Barcelona, Barcelona, Spain
    J Mol Biol 302:1227-40. 2000
    ..Assay of Lpro activity on a fluorescent hexapeptide substrate showed that Lpro, in contrast to papain, was highly sensitive to increases in the cation concentration and was active only across a narrow pH range...
  92. pmc A zinc finger-containing papain-like protease couples subgenomic mRNA synthesis to genome translation in a positive-stranded RNA virus
    M A Tijms
    Department of Virology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands
    Proc Natl Acad Sci U S A 98:1889-94. 2001
    ..Nsp1 is composed of two papain-like protease domains and a predicted N-terminal zinc finger, which was implicated in transcription by site-..
  93. pmc Sulfonation of papain-treated chitosan and its mechanism for anticoagulant activity
    Jiraporn Suwan
    Thailand Excellence Center for Tissue Engineering, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
    Carbohydr Res 344:1190-6. 2009
    ..chitosan polysulfate (MW 5120-26,200 Da) was prepared using the depolymerization of chitosan with papain (EC. 3.4.22.2)...
  94. ncbi The thiol proteinases from the latex of Carica papaya L. I. Fractionation, purification and preliminary characterization
    T Dubois
    Laboratoire de Chimie Générale I, université de Bruxelles
    Biol Chem Hoppe Seyler 369:733-40. 1988
    Three thiol proteinases, namely papain, chymopapain and proteinase omega were purified to homogeneity from the latex of Carica papaya L...
  95. ncbi The role of Gly-4 of human cystatin A (stefin A) in the binding of target proteinases. Characterization by kinetic and equilibrium methods of the interactions of cystatin A Gly-4 mutants with papain, cathepsin B, and cathepsin L
    S Estrada
    Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala Biomedical Center
    Biochemistry 37:7551-60. 1998
    ..by Ala, resulted in approximately 1000-, approximately 10- and approximately 6000-fold decreased affinities for papain, cathepsin L, and cathepsin B, respectively...
  96. ncbi The contribution of N-terminal region residues of cystatin A (stefin A) to the affinity and kinetics of inhibition of papain, cathepsin B, and cathepsin L
    S Estrada
    Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala Biomedical Center
    Biochemistry 38:7339-45. 1999
    ..However, deletion also of Ile-2 resulted in reduced affinities of 900-, >/=3-, and 200-fold for papain and cathepsins L and B, respectively...
  97. doi Mesenchymal stem cell transplantation increases expression of vascular endothelial growth factor in papain-induced emphysematous lungs and inhibits apoptosis of lung cells
    Guohua Zhen
    Division of Respiratory Diseases, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China
    Cytotherapy 12:605-14. 2010
    ..We have found that bone marrow (BM) mesenchymal stem cell (MSC) transplantation ameliorates papain-induced pulmonary emphysema. However, the underlying mechanism is not completely understood...
  98. ncbi Molecular cloning of a protein associated with soybean seed oil bodies that is similar to thiol proteases of the papain family
    A Kalinski
    Plant Molecular Biology Laboratory, United States Department of Agriculture, Beltsville, Maryland 20705
    J Biol Chem 265:13843-8. 1990
    ..sequences in the GenBank Data Bank with the sequence of P34 has shown considerable sequence similarity to the thiol proteases of the papain family. Southern blot analysis of genomic DNA indicated that the P34 gene has a low copy number.
  99. ncbi The H1 double-stranded RNA genome of Ustilago maydis virus-H1 encodes a polyprotein that contains structural motifs for capsid polypeptide, papain-like protease, and RNA-dependent RNA polymerase
    J Kang
    Kumho Life and Environmental Science Laboratory, 1 Oryong-dong, Buk-gu, 500-480, Kwangju, South Korea
    Virus Res 76:183-9. 2001
    ..frame that encodes a polyprotein of 1820 residues, which is predicted to be autocatalytically processed by a viral papain-like protease to generate viral proteins...
  100. pmc The refined 2.4 A X-ray crystal structure of recombinant human stefin B in complex with the cysteine proteinase papain: a novel type of proteinase inhibitor interaction
    M T Stubbs
    Max Planck Institut fur Biochemie, Martinsried bei Munchen, FRG
    EMBO J 9:1939-47. 1990
    A stoichiometric complex of human stefin B and carboxymethylated papain has been crystallized in a trigonal crystal form. Data to 2.37 A resolution were collected using the area detector diffractometer FAST...
  101. ncbi Purification of Fab fragments from a monoclonal antibody papain digest by Gradiflow electrophoresis
    Lynette Coleman
    Bioengineering Centre, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia
    Protein Expr Purif 32:246-51. 2003
    Fab fragments isolated from papain digests of monoclonal antibodies have a wide variety of uses in analytical and in both in vivo and in vitro diagnostic applications...

Research Grants68

  1. Mechanisms of viral proteases in coronavirus replication and pathogenesis
    Andrew D Mesecar; Fiscal Year: 2010
    ..Previously, we identified the papain-like protease (PLpro) of SARS-CoV as a critical determinant of viral replication and pathogenesis...
  2. TRANSPORT MECHANISMS IN THE HUMAN SMALL INTESTINE
    Krishnamurthy Ramaswamy; Fiscal Year: 1990
    ..Control, papain-and inhibitor (peptidase)-treated membrane vesicles will be employed for these studies...
  3. TRANSPORT MECHANISMS IN THE HUMAN SMALL INTESTINE
    Krishnamurthy Ramaswamy; Fiscal Year: 1992
    ..Control, papain-and inhibitor (peptidase)-treated membrane vesicles will be employed for these studies...
  4. TRANSPORT MECHANISMS IN THE HUMAN SMALL INTESTINE
    Krishnamurthy Ramaswamy; Fiscal Year: 1993
    ..Control, papain-and inhibitor (peptidase)-treated membrane vesicles will be employed for these studies...
  5. TRANSPORT MECHANISMS IN THE HUMAN SMALL INTESTINE
    Krishnamurthy Ramaswamy; Fiscal Year: 1991
    ..Control, papain-and inhibitor (peptidase)-treated membrane vesicles will be employed for these studies...
  6. Abnormality in Gene Expression of Key Mediators of Vitamin A Action in COPD
    SALIL DAS; Fiscal Year: 2009
    ..RA is known to be effective in promoting alveolization in papain-induced emphysema in dogs and elastase-induced emphysema in adult rats...
  7. Abnormality in Gene Expression of Key Mediators of Vitamin A Action in COPD
    Salil K Das; Fiscal Year: 2010
    ..RA is known to be effective in promoting alveolization in papain-induced emphysema in dogs and elastase-induced emphysema in adult rats...
  8. CELL SURFACE ANTIGENS ON HUMAN MACROPHAGES
    Robert Todd; Fiscal Year: 1990
    ..two-subunit surface protein (p 40, 46) found on macrophages isolated from human colostrum, while PAM1 is a papain-sensitive 200 kilodalton polypeptide uniquely expressed by pulmonary alveolar macrophages...
  9. CELL SURFACE ANTIGENS OF HUMAN MACROPHAGES
    Robert Todd; Fiscal Year: 1993
    ..two-subunit surface protein (p 40, 46) found on macrophages isolated from human colostrum, while PAM1 is a papain-sensitive 200 kilodalton polypeptide uniquely expressed by pulmonary alveolar macrophages...
  10. DESIGN AND SYNTHESIS OF CYSTEINE PROTEINASE INHIBITORS
    DANIEL RICH; Fiscal Year: 1990
    ..for new peptidyl semicarbazones, aldehydes, and nitriles for inhibition of the following cysteine proteinases: papain; cathepsin B, H, and L; the cysteine proteinase for entamoeba histolytica; and calpains I and II...
  11. Role of Red Cell Membrane in Malaria Parasite Release
    Manjit Hanspal; Fiscal Year: 2002
    ..series of peptides derived from Pi in which critical residues are varied based on substrate specificities of other papain family enzymes...
  12. Role of Red Cell Membrane in Malaria Parasite Release
    Manjit Hanspal; Fiscal Year: 2003
    ..series of peptides derived from Pi in which critical residues are varied based on substrate specificities of other papain family enzymes...
  13. Role of Red Cell Membrane in Malaria Parasite Release
    Manjit Hanspal; Fiscal Year: 2004
    ..series of peptides derived from Pi in which critical residues are varied based on substrate specificities of other papain family enzymes...
  14. Role of Red Cell Membrane in Malaria Parasite Release
    Manjit Hanspal; Fiscal Year: 2005
    ..series of peptides derived from Pi in which critical residues are varied based on substrate specificities of other papain family enzymes...
  15. Role of Red Cell Membrane in Malaria Parasite Release
    Manjit Hanspal; Fiscal Year: 2006
    ..series of peptides derived from Pi in which critical residues are varied based on substrate specificities of other papain family enzymes...
  16. CHARACTERIZATION OF THE PEMPHIGUS FOLIACEUS ANTIGEN
    Grant Anhalt; Fiscal Year: 1991
    ..An immunoreactive soluble 50 KD papain fragment of this PF Ag (pf-PF) could be released into solution and specifically immunoprecipitated by PF sera...
  17. CARBOHYDRATE-CONTAINING MACROMOLECULES IN AGING BRAIN
    ERIC BRUNNGRABER; Fiscal Year: 1980
    ..The defatted residue is treated with papain to solubilize glycopeptides and glycosaminoglycans...
  18. STRUCTURE AND FUNCTION OF CARBAMYL PHOSPHATE SYNTHETASE
    CAROL LUSTY; Fiscal Year: 1993
    ..intermediate during the hydrolysis of glutamine and the observed sequence homology of the alpha subunit to papain. This model will be experimentally tested through studies of site-directed mutations in residues conserved among ..
  19. SPINAL CORD CELL EXCITABILITY
    Raul Mandler; Fiscal Year: 1990
    ..Wobbler (NFR/wr) and control (NFR) spinal cords will be dissected in 6 regions and cells will be isolated using a papain dissociation protocol...
  20. Biochemical Characterization of Yersinia Effector YopJ
    Kim Orth; Fiscal Year: 2010
    ..properties of four other Yersinia effectors: YopH, a tyrosine phosphatase;YopE, a GAP;YpkA, a serine kinase;YopT, papain-like protease...
  21. Biochemical Characterization of Yersinia Effector YopJ
    Kim Orth; Fiscal Year: 2009
    ..properties of four other Yersinia effectors: YopH, a tyrosine phosphatase;YopE, a GAP;YpkA, a serine kinase;YopT, papain-like protease...
  22. ELECTRON MICROSCOPY OF HUMAN ALPHA-2 MACROGLOBULIN
    JAMES STOOPS; Fiscal Year: 1993
    ..in which proteinases are bound to alpha2M should result from determining the orientation of the active site of papain labelled with a gold cluster in the complex...
  23. REACTION MECHANISMS FOR ENZYMES
    JULES SHAFER; Fiscal Year: 1990
    b>Papain, a thiol protease from papaya latex, will be studied to determine the role of Asp-158 in catalysis and to determine the effect of Asp-158 on the interactive ionization of His-159 and Cys-25 at the active site of papain...
  24. CORONAVIRUSES--MOLECULAR BIOLOGY AND PERSISTENCE
    Susan Weiss; Fiscal Year: 1999
    ..domains in ORF 1a (1) cDNA clones containing portions of ORF 1a will be used to analyze the two predicted papain-like proteinase domains, PLP-1 and PLP-2...
  25. BIOCHEMICAL STUDIES OF ERYTHOCYTE ANION TRANSPORT
    Michael Jennings; Fiscal Year: 1980
    ..The activity of the protein is inhibited by extracellular proteolysis with papain. The molecular origin of the inhibition will be investigated by characterizing the products of extracellular ..
  26. NEW CLASS OF SERINE AND CYSTEINE PROTEASE INHIBITORS
    CHRISTOPHER SETO; Fiscal Year: 1999
    ..this inhibitor design, a series of 4- heterocyclohexanone-based inhibitors of the prototypical cysteine protease papain have been synthesized...
  27. NEW CLASS OF SERINE AND CYSTEINE PROTEASE INHIBITORS
    CHRISTOPHER SETO; Fiscal Year: 2002
    ..this inhibitor design, a series of 4- heterocyclohexanone-based inhibitors of the prototypical cysteine protease papain have been synthesized...
  28. NEW CLASS OF SERINE AND CYSTEINE PROTEASE INHIBITORS
    CHRISTOPHER SETO; Fiscal Year: 2001
    ..this inhibitor design, a series of 4- heterocyclohexanone-based inhibitors of the prototypical cysteine protease papain have been synthesized...
  29. NEW CLASS OF SERINE AND CYSTEINE PROTEASE INHIBITORS
    CHRISTOPHER SETO; Fiscal Year: 2000
    ..this inhibitor design, a series of 4- heterocyclohexanone-based inhibitors of the prototypical cysteine protease papain have been synthesized...
  30. CHEMISTRY AND BIOCHEMISTRY OF NO RELEASING AGENTS
    Peng Wang; Fiscal Year: 1999
    ..of the N-nitrosoaniline moiety into a peptide backbone and synthesis of specific S-nitrosating agents to inhibit papain, interleukin-1beta-converting enzyme (ICE) and protein tyrosine phosphatases (PTP)...
  31. CHEMISTRY AND BIOCHEMISTRY OF NO RELEASING AGENTS
    Peng Wang; Fiscal Year: 2001
    ..of the N-nitrosoaniline moiety into a peptide backbone and synthesis of specific S-nitrosating agents to inhibit papain, interleukin-1beta-converting enzyme (ICE) and protein tyrosine phosphatases (PTP)...
  32. CHEMISTRY AND BIOCHEMISTRY OF NO RELEASING AGENTS
    Peng Wang; Fiscal Year: 2000
    ..of the N-nitrosoaniline moiety into a peptide backbone and synthesis of specific S-nitrosating agents to inhibit papain, interleukin-1beta-converting enzyme (ICE) and protein tyrosine phosphatases (PTP)...
  33. REGULATION OF ADENOVIRUS PROTEINASE BY A PEPTIDE AND DNA
    WALTER MANGEL; Fiscal Year: 2000
    ..A putative active site contains a Cys-His-Glu triplet and oxyanion hole in an arrangement similar to that in papain. If that is the active site, this protein would represent a new class of cysteine proteinases and a new, fifth ..
  34. REGULATION OF ADENOVIRUS PROTEINASE BY A PEPTIDE AND DNA
    WALTER MANGEL; Fiscal Year: 1999
    ..A putative active site contains a Cys-His-Glu triplet and oxyanion hole in an arrangement similar to that in papain. If that is the active site, this protein would represent a new class of cysteine proteinases and a new, fifth ..
  35. CELLULAR AND STRUCTURAL STUDIES OF MYELOMA MUTANTS
    BARBARA BIRSHTEIN; Fiscal Year: 1980
    ..These same parameters have enabled us to subgroup the variants. Analytical techniques of papain digestion products such as immunoelectrophoresis, SDS-PAGE of CNBr fragments, and comparative peptide maps, in ..
  36. Role of Elastolytic Cathepsins in Emphysema
    Harold A Chapman; Fiscal Year: 2010
    Accumulating evidence indicates papain-family cysteine proteases are involved in the pathobiology of emphysema...
  37. Role of Elastolytic Cathepsins in Emphysema
    Harold Chapman; Fiscal Year: 2009
    Accumulating evidence indicates papain-family cysteine proteases are involved in the pathobiology of emphysema...
  38. Role of Elastolytic Cathepsins in Emphysema
    Harold Chapman; Fiscal Year: 2007
    Accumulating evidence indicates papain-family cysteine proteases are involved in the pathobiology of emphysema...
  39. Role of Elastolytic Cathepsins in Emphysema
    Harold Chapman; Fiscal Year: 2006
    EXCEED THE SPACE PROVIDED. Accumulating evidence indicates papain-family cysteine proteases are involved in the pathobiology of emphysema...
  40. STRUCTURAL AND BIOSYNTHETIC STUDIES OF HL-A DETERMINANTS
    JACK STROMINGER; Fiscal Year: 1980
    ..B, -C, and -D locus products) from the surface of cultured human lymphocyte cell lines, using both detergent and papain solubilization; 2) to carry out structural studies on purified HLA antigens in order to examine the question of ..
  41. Role of basophils in initiating Th2 immune responses
    Ruslan Medzhitov; Fiscal Year: 2010
    ..Using a protease allergen papain as a model system, we have recently demonstrated that basophils play an essential role in allergen-induced Th2 ..
  42. Falcipain Cysteine Proteases of Malaria Parasites
    Philip Rosenthal; Fiscal Year: 2005
    ..project, important advances have been made in our understanding of this class of enzymes, and in particular in the papain-family proteases known as falcipains...
  43. NEURAL CONTROL OF BREATHING IN PAPAIN INDUCED EMPHYSEMA
    Steven Kelsen; Fiscal Year: 1980
    ..the physiologic features of chronic obstructive lung disease will be produced in dogs by the administration of papain. The lung and chest wall mechanoreceptors will be assessed in conscious and anesthetized animals before and ..
  44. CARBONYLS IN ENZYME MECHANISM--RAMAN CHARACTERIZATION
    Paul Carey; Fiscal Year: 1999
    ..proteases the specific aims are: * For the mammalian proteases, cathepsins B and L (together with the plant enzyme papain) and the hepatitis viral protease HAV-3C, and their protein- engineered analogs, to use the carbonyl stretching ..
  45. Novel Protease Inhibitors as SARS Therapeutics
    Michael Johnson; Fiscal Year: 2005
    ..The replicase polyprotein is proteolytically processed by two viral proteases, papain-like protease 2 (PLpro) and 3CLpro...
  46. Novel Protease Inhibitors as SARS Therapeutics
    Michael Johnson; Fiscal Year: 2009
    ..The replicase polyprotein is proteolytically processed by two viral proteases, papain-like protease 2 (PLpro) and 3CLpro...
  47. Novel Protease Inhibitors as SARS Therapeutics
    Michael Johnson; Fiscal Year: 2007
    ..The replicase polyprotein is proteolytically processed by two viral proteases, papain-like protease 2 (PLpro) and 3CLpro...
  48. Novel Protease Inhibitors as SARS Therapeutics
    Michael Johnson; Fiscal Year: 2006
    ..The replicase polyprotein is proteolytically processed by two viral proteases, papain-like protease 2 (PLpro) and 3CLpro...
  49. STRUCTURAL CHARACTERIZATION-LIVER ALDEHYDE DEHYDROGENASE
    John Hempel; Fiscal Year: 1990
    ..crosslinking an internal salt bridge in Class 1/2 AlDHs suggested from alignment of AlDHs with thiol proteases and papain crystal structure correlations. Concurrently, site-directed mutagenesis on this A1DH will also be performed...
  50. STRUCTURAL CHARACTERIZATION-LIVER ALDEHYDE DEHYDROGENASE
    John Hempel; Fiscal Year: 1992
    ..crosslinking an internal salt bridge in Class 1/2 AlDHs suggested from alignment of AlDHs with thiol proteases and papain crystal structure correlations. Concurrently, site-directed mutagenesis on this A1DH will also be performed...
  51. BIOCHEMICAL STUDIES ON HISTOCOMPATIBILITY ANTIGENS
    STANLEY NATHENSON; Fiscal Year: 1980
    ..Sequence analysis of the five fragments comprising the antigenically active papain cleaved molecule has been carried out...
  52. BORONIC ACID ANALOGS OF PEPTIDES
    Robert Lindquist; Fiscal Year: 1980
    ..Inhibitors are proposed for the following enzymes: (1) elastase, thought to be involved in emphysema; (2) papain, a sulfhydryl enzyme; and (3) thermolysin, a proteolytic enzyme involved in a number of important physiological ..
  53. IMMUNOGENETICS OF THE TLA REGION OF CHROMOSOME 17
    EDWARD BOYSE; Fiscal Year: 1980
    ..Restricted proteolysis with trypsin and papain will be used to isolate surface-disposed H-chain pieces...
  54. Functions of Ub-like Proteins and Proteases
    KEITH WILKINSON; Fiscal Year: 2009
    ..We will determine the enzymatic specificity of the DUB activity exhibited by the SARS virus papain-like protease using the panel of UBL-X reagents...
  55. Functions of Ub-like Proteins and Proteases
    KEITH WILKINSON; Fiscal Year: 2006
    ..We will determine the enzymatic specificity of the DUB activity exhibited by the SARS virus papain-like protease using the panel of UBL-X reagents...
  56. Functions of Ub-like Proteins and Proteases
    KEITH WILKINSON; Fiscal Year: 2007
    ..We will determine the enzymatic specificity of the DUB activity exhibited by the SARS virus papain-like protease using the panel of UBL-X reagents...
  57. POLYMERASE PROTEINS IN CORONAVIRUS REPLICATION
    Mark Denison; Fiscal Year: 2007
    ..p65) has known functions, while nsp3 (p210) is predicted to be a multifunctional protein that contains two known papain-like proteinases (PLP1 and PLP2) as well as other domains with possible functions in RNA synthesis and replication ..