mannosidases

Summary

Summary: Glycoside hydrolases that catalyze the hydrolysis of alpha or beta linked MANNOSE.

Top Publications

  1. pmc Efficient recombinant expression and secretion of a thermostable GH26 mannan endo-1,4-beta-mannosidase from Bacillus licheniformis in Escherichia coli
    Chomphunuch Songsiriritthigul
    School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Nakhon Ratchasima, Thailand
    Microb Cell Fact 9:20. 2010
  2. pmc Golgi localization of ERManI defines spatial separation of the mammalian glycoprotein quality control system
    Shujuan Pan
    Departments of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Biol Cell 22:2810-22. 2011
  3. pmc In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris
    Wouter Vervecken
    Department of Molecular Biomedical Research, Ghent University and Flanders Interuniversity Institute for Biotechnology, Ghent, Belgium
    Appl Environ Microbiol 70:2639-46. 2004
  4. ncbi Dissecting glycoprotein quality control in the secretory pathway
    C M Cabral
    Dept of Pathology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Trends Biochem Sci 26:619-24. 2001
  5. ncbi LeMAN4 endo-beta-mannanase from ripe tomato fruit can act as a mannan transglycosylase or hydrolase
    Roswitha Schröder
    Mt Albert Research Centre, The Horticulture and Food Research Institute of New Zealand, Private Bag 92 169, Auckland, New Zealand
    Planta 224:1091-102. 2006
  6. pmc Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-beta-mannosidase from Aspergillus niger BK01
    Bien Cuong Do
    School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Nakhon Ratchasima 30000, Thailand
    Microb Cell Fact 8:59. 2009
  7. pmc Mannose trimming is required for delivery of a glycoprotein from EDEM1 to XTP3-B and to late endoplasmic reticulum-associated degradation steps
    Bella Groisman
    Department of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    J Biol Chem 286:1292-300. 2011
  8. pmc Endoplasmic reticulum (ER) mannosidase I is compartmentalized and required for N-glycan trimming to Man5-6GlcNAc2 in glycoprotein ER-associated degradation
    Edward Avezov
    Department of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Mol Biol Cell 19:216-25. 2008
  9. ncbi A complex of Pdi1p and the mannosidase Htm1p initiates clearance of unfolded glycoproteins from the endoplasmic reticulum
    Robert Gauss
    Institute of Microbiology, Department of Biology, Swiss Federal Institute of Technology Zurich, 8093 Zurich, Switzerland
    Mol Cell 42:782-93. 2011
  10. ncbi Cloning and expression of a specific human alpha 1,2-mannosidase that trims Man9GlcNAc2 to Man8GlcNAc2 isomer B during N-glycan biosynthesis
    L O Tremblay
    McGill Cancer Centre, Montreal, Quebec, Canada
    Glycobiology 9:1073-8. 1999

Detail Information

Publications192 found, 100 shown here

  1. pmc Efficient recombinant expression and secretion of a thermostable GH26 mannan endo-1,4-beta-mannosidase from Bacillus licheniformis in Escherichia coli
    Chomphunuch Songsiriritthigul
    School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Nakhon Ratchasima, Thailand
    Microb Cell Fact 9:20. 2010
    ..licheniformis...
  2. pmc Golgi localization of ERManI defines spatial separation of the mammalian glycoprotein quality control system
    Shujuan Pan
    Departments of Pathology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Biol Cell 22:2810-22. 2011
    ..Taken together, these data provide a potential mechanistic explanation for the spatial separation by which glycoprotein quality control components operate in mammalian cells...
  3. pmc In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris
    Wouter Vervecken
    Department of Molecular Biomedical Research, Ghent University and Flanders Interuniversity Institute for Biotechnology, Ghent, Belgium
    Appl Environ Microbiol 70:2639-46. 2004
    ..The procedures which we developed allow glycan engineering of any P. pastoris expression strain and can yield up to 90% homogeneous protein-linked oligosaccharides...
  4. ncbi Dissecting glycoprotein quality control in the secretory pathway
    C M Cabral
    Dept of Pathology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Trends Biochem Sci 26:619-24. 2001
    ..That the mechanisms by which glycoprotein substrates are recruited for degradation diverge at the level of signal recognition reflects a previously unappreciated component of cellular differentiation in higher eukaryotes...
  5. ncbi LeMAN4 endo-beta-mannanase from ripe tomato fruit can act as a mannan transglycosylase or hydrolase
    Roswitha Schröder
    Mt Albert Research Centre, The Horticulture and Food Research Institute of New Zealand, Private Bag 92 169, Auckland, New Zealand
    Planta 224:1091-102. 2006
    ....
  6. pmc Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-beta-mannosidase from Aspergillus niger BK01
    Bien Cuong Do
    School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Nakhon Ratchasima 30000, Thailand
    Microb Cell Fact 8:59. 2009
    ..Mannan endo-1,4-beta-mannosidases (1,4-beta-D-mannanases) catalyze the random hydrolysis of beta-1,4-mannosidic linkages in the main chain of ..
  7. pmc Mannose trimming is required for delivery of a glycoprotein from EDEM1 to XTP3-B and to late endoplasmic reticulum-associated degradation steps
    Bella Groisman
    Department of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    J Biol Chem 286:1292-300. 2011
    ..Our results suggest that mannose trimming enables delivery of a substrate glycoprotein from EDEM1 to late ERAD steps through association with XTP3-B...
  8. pmc Endoplasmic reticulum (ER) mannosidase I is compartmentalized and required for N-glycan trimming to Man5-6GlcNAc2 in glycoprotein ER-associated degradation
    Edward Avezov
    Department of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Mol Biol Cell 19:216-25. 2008
    ..When newly made glycoproteins cannot fold properly, transport through the ERQC leads to trimming of a critical number of mannose residues, triggering a signal for degradation...
  9. ncbi A complex of Pdi1p and the mannosidase Htm1p initiates clearance of unfolded glycoproteins from the endoplasmic reticulum
    Robert Gauss
    Institute of Microbiology, Department of Biology, Swiss Federal Institute of Technology Zurich, 8093 Zurich, Switzerland
    Mol Cell 42:782-93. 2011
    Endoplasmic reticulum (ER)-resident mannosidases generate asparagine-linked oligosaccharide signals that trigger ER-associated protein degradation (ERAD) of unfolded glycoproteins...
  10. ncbi Cloning and expression of a specific human alpha 1,2-mannosidase that trims Man9GlcNAc2 to Man8GlcNAc2 isomer B during N-glycan biosynthesis
    L O Tremblay
    McGill Cancer Centre, Montreal, Quebec, Canada
    Glycobiology 9:1073-8. 1999
    ..5 kDa with amino acid sequence similarity to Class I alpha 1,2-mannosidases. The catalytic domain of the enzyme was expressed as a secreted protein in Pichia pastoris...
  11. ncbi Identification, expression, and characterization of a cDNA encoding human endoplasmic reticulum mannosidase I, the enzyme that catalyzes the first mannose trimming step in mammalian Asn-linked oligosaccharide biosynthesis
    D S Gonzalez
    Complex Carbohydrate Research Center and the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 274:21375-86. 1999
    ..sequence tag clones were identified by sequence similarity to mammalian and yeast oligosaccharide-processing mannosidases, and the full-length coding region of the putative mannosidase homolog was isolated by a combination of 5'-..
  12. ncbi Glycoprotein biosynthesis in Saccharomyces cerevisiae. Isolation and characterization of the gene encoding a specific processing alpha-mannosidase
    A Camirand
    McGill Cancer Centre, McGill University, Montreal, Quebec, Canada
    J Biol Chem 266:15120-7. 1991
    ..These results demonstrate that MNS1 is the structural gene for the specific alpha-mannosidase and that its activity is not essential for viability...
  13. pmc Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans
    Yunkyoung Song
    School of Bioscience and Biotechnology, Chungnam National University, Daejeon 305 764, Korea
    Appl Environ Microbiol 73:4446-54. 2007
    ..Therefore, the Yloch1 mutant strain can be used as a host to produce glycoproteins lacking the outer-chain mannoses and further developed for the production of therapeutic glycoproteins containing human-compatible oligosaccharides...
  14. ncbi Functional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastoris
    Robert C Davidson
    GlycoFi, Inc, 21 Lafayette Street Suite 200, Lebanon, NH 03766 Velocity 11 435 Acacia Ave, Palo Alto, CA 94306, USA
    Glycobiology 14:399-407. 2004
    ..pastoris alg3 deletion mutant in the presence and absence of the outer chain initiating Och1p alpha-1,6-mannosyltransferase activity suggests that the PpOch1p has a broader substrate specificity compared to its S. cerevisiae counterpart...
  15. pmc Single nucleotide polymorphism-mediated translational suppression of endoplasmic reticulum mannosidase I modifies the onset of end-stage liver disease in alpha1-antitrypsin deficiency
    Shujuan Pan
    Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA
    Hepatology 50:275-81. 2009
    ..Functional studies indicated that rs4567(A), but not rs4567(G), suppresses ERManI translation under ER stress conditions...
  16. ncbi A time-dependent phase shift in the mammalian unfolded protein response
    Hiderou Yoshida
    Graduate School of Biostudies, Kyoto University, Kyoto 606 8304, Japan
    Dev Cell 4:265-71. 2003
    ....
  17. pmc GS15 forms a SNARE complex with syntaxin 5, GS28, and Ykt6 and is implicated in traffic in the early cisternae of the Golgi apparatus
    Yue Xu
    Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Singapore 117609, Singapore
    Mol Biol Cell 13:3493-507. 2002
    ....
  18. pmc Variation in its C-terminal amino acids determines whether endo-beta-mannanase is active or inactive in ripening tomato fruits of different cultivars
    Richard Bourgault
    Department of Botany, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    Plant Physiol 130:1254-62. 2002
    ..Site-directed mutagenesis was used to establish key elements in the C terminus of the endo-beta-mannanase protein that are essential for full enzyme activity...
  19. ncbi Use of HDEL-tagged Trichoderma reesei mannosyl oligosaccharide 1,2-alpha-D-mannosidase for N-glycan engineering in Pichia pastoris
    N Callewaert
    Unit of Fundamental and Applied Molecular Biology, Department of Molecular Biology, Ghent University, Ghent, Belgium
    FEBS Lett 503:173-8. 2001
    ..Moreover, the human-type high-mannose oligosaccharide Man(5)GlcNAc(2) was the major N-glycan of the glyco-engineered trans-sialidase, indicating that N-glycan engineering can be effectively accomplished in P. pastoris...
  20. pmc Elucidation of the molecular logic by which misfolded alpha 1-antitrypsin is preferentially selected for degradation
    Ying Wu
    Department of Pathology, Baylor College of Medicine, Houston, TX 77030 3498, USA
    Proc Natl Acad Sci U S A 100:8229-34. 2003
    ..Taken together, these findings indicate that efficient conformational maturation might function as the initial quality control standard for a broad population of glycoproteins...
  21. ncbi Three endo-β-mannanase genes expressed in the micropylar endosperm and in the radicle influence germination of Arabidopsis thaliana seeds
    Raquel Iglesias-Fernández
    Departamento de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Santiago de Compostela, Santiago de Compostela, Spain
    Planta 233:25-36. 2011
    ..These data suggest that AtMAN7, AtMAN5 and specially AtMAN6 are important for the germination of A. thaliana seeds by facilitating the hydrolysis of the mannan-rich endosperm cell walls...
  22. ncbi Microbial mannanases: an overview of production and applications
    Samriti Dhawan
    Department of Biotechnology, GGDSD College, Chandigarh, India
    Crit Rev Biotechnol 27:197-216. 2007
    ..The reference to plant and animal mannanases has been made to complete the overview. However, the major emphasis of the review is on the microbial mannanases...
  23. pmc Degradation of misfolded endoplasmic reticulum glycoproteins in Saccharomyces cerevisiae is determined by a specific oligosaccharide structure
    C A Jakob
    Division of Cell and Molecular Pathology, Department of Pathology, University of Zurich, CH 8091 Zurich, Switzerland
    J Cell Biol 142:1223-33. 1998
    ..These results suggest a role for the Man8GlcNAc2 oligosaccharide in the degradation process. They may indicate the presence of a Man8GlcNAc2-binding lectin involved in targeting of misfolded glycoproteins to degradation in S. cerevisiae...
  24. pmc A novel ER alpha-mannosidase-like protein accelerates ER-associated degradation
    N Hosokawa
    Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8397, Japan
    EMBO Rep 2:415-22. 2001
    ..luminal region of ER degradation enhancing alpha-mannosidase-like protein (EDEM) is similar to class I alpha1,2-mannosidases involved in N-glycan processing, EDEM did not have enzymatic activity...
  25. ncbi Structural basis for catalysis and inhibition of N-glycan processing class I alpha 1,2-mannosidases
    F Vallee
    Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 275:41287-98. 2000
    ..The structures also provide insight into the specificity of this class of enzymes and provide a blueprint for the future design of novel inhibitors that prevent degradation of misfolded proteins in genetic diseases...
  26. ncbi Endo-beta-mannosidase, a plant enzyme acting on N-glycan: purification, molecular cloning, and characterization
    Takeshi Ishimizu
    Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    J Biol Chem 279:38555-62. 2004
    ..acid sequence of AtEBM has distinct differences from those of the bacterial, fungal, and animal exo-type beta-mannosidases. Indeed, AtEBM-like genes are only found in plants, indicating that endo-beta-mannosidase is a plant-specific ..
  27. ncbi The transmembrane domain of murine alpha-mannosidase IB is a major determinant of Golgi localization
    B Becker
    McGill Cancer Centre, McGill University Montreal, Quebec, Canada
    Eur J Cell Biol 79:986-92. 2000
    ..A GFP chimera lacking the entire cytoplasmic tail was found in both the ER and the Golgi. These results indicate that the transmembrane domain of alpha1,2-mannosidase IB is a major determinant of Golgi localization...
  28. pmc Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans
    K W Moremen
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139
    J Cell Biol 115:1521-34. 1991
    ..Partial human alpha-mannosidase II cDNA clones were also isolated and the gene was localized to human chromosome 5...
  29. pmc Probing for membrane domains in the endoplasmic reticulum: retention and degradation of unassembled MHC class I molecules
    Elias T Spiliotis
    Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Mol Biol Cell 13:1566-81. 2002
    ..Microtubules were required for its appearance. Segregation of unfolded proteins, ER-resident chaperones, and COPII may be a temporal adaptation to cell stress...
  30. ncbi The alpha-mannosidases: phylogeny and adaptive diversification
    D S Gonzalez
    Department of Medical Microbiology, University of Georgia, USA
    Mol Biol Evol 17:292-300. 2000
    ..These data point to a possible role for positive Darwinian selection in the evolution of alpha-mannosidase functional diversification following gene duplication...
  31. pmc Disruption of the processing alpha-mannosidase gene does not prevent outer chain synthesis in Saccharomyces cerevisiae
    R Puccia
    McGill Cancer Centre, McGill University, Montreal, Quebec, Canada
    Biochem J 290:21-6. 1993
    ..It is concluded that the alpha-mannosidase encoded by MNS1 is the only enzyme responsible for mannose removal in vivo, and that this processing step is not essential for outer chain synthesis...
  32. ncbi Molecular cloning of a cDNA encoding a (1-->4)-beta-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum)
    J D Bewley
    Department of Botany, University of Guelph, Ontario, Canada
    Planta 203:454-9. 1997
    ..Expression of the genes, as determined by the presence of mRNA transcripts in Northern hybridization analyses, occurs in the endosperm of germinated seeds; no transcripts are detected in hypocotyls, cotyledons, roots or leaves...
  33. ncbi Molecular cloning and primary structure of Man9-mannosidase from human kidney
    E Bause
    Institut fur Physiologische Chemie, Universitat Bonn, Germany
    Eur J Biochem 217:535-40. 1993
    ..This finding may indicate that the mammalian alpha-mannosidases, which differ significantly in their substrate specificity, are coded for by evolutionarily unrelated genes, ..
  34. ncbi Molecular cloning, chromosomal mapping and tissue-specific expression of a novel human alpha1,2-mannosidase gene involved in N-glycan maturation
    L O Tremblay
    McGill Cancer Centre, McGill University, 3655 Drummond Street, Montreal, Quebec, Canada H3G 1Y6
    Glycobiology 8:585-95. 1998
    Class I alpha1,2-mannosidases play an essential role in the elaboration of complex and hybrid N -glycans in mammalian cells...
  35. ncbi Endo-beta-mannanase activity increases in the skin and outer pericarp of tomato fruits during ripening
    J D Bewley
    Department of Botany, University of Guelph, Ontario, Canada
    J Exp Bot 51:529-38. 2000
    ....
  36. ncbi Enhancement of endoplasmic reticulum (ER) degradation of misfolded Null Hong Kong alpha1-antitrypsin by human ER mannosidase I
    Nobuko Hosokawa
    Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8397, Japan
    J Biol Chem 278:26287-94. 2003
    ....
  37. pmc A temperature-sensitive dcw1 mutant of Saccharomyces cerevisiae is cell cycle arrested with small buds which have aberrant cell walls
    Hiroshi Kitagaki
    National Research Institute of Brewing, 3 7 1, Kagamiyama, Higashihiroshima, Hiroshima, Japan 739 0046
    Eukaryot Cell 3:1297-306. 2004
    ..DCW1 and DFG5 mRNA levels peaked in the G1 and S phases, respectively. These results indicate that Dcw1p and Dfg5p are involved in bud formation through their involvement in biogenesis of the bud cell wall...
  38. ncbi High-resolution native and complex structures of thermostable beta-mannanase from Thermomonospora fusca - substrate specificity in glycosyl hydrolase family 5
    M Hilge
    Laboratorium für Biochemie ETH Zentrum Universitätstrasse 16 CH 8092 Zürich, Switzerland
    Structure 6:1433-44. 1998
    ..No three-dimensional structure of a mannan-degrading enzyme has been reported until now...
  39. ncbi Cell biology. Protein degradation unlocked
    Richard N Sifers
    Departments of Pathology, and Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 299:1330-1. 2003
  40. ncbi Lectin-like ERAD players in ER and cytosol
    Yukiko Yoshida
    Laboratory of Frontier Science, The Tokyo Metropolitan Institute of Medical Science, 2 1 6, Kamikitazawa, Setagaya Ku, Tokyo, 156 8506, Japan
    Biochim Biophys Acta 1800:172-80. 2010
    ..In this review, we discuss a fundamental system that monitors glycoprotein folding in the ER and the unique roles of the sugar-recognizing ubiquitin ligase and peptide:N-glycanase (PNGase) in the cytosolic ERAD pathway...
  41. pmc Plant N-glycan processing enzymes employ different targeting mechanisms for their spatial arrangement along the secretory pathway
    Claude Saint-Jore-Dupas
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6037, IFRMP 23, GDR 2590, UFR des Sciences, Universite de Rouen, 76821 Mont Saint Aignan Cedex, France
    Plant Cell 18:3182-200. 2006
    ..We conclude that the localization of N-glycan processing enzymes corresponds to an assembly line in the early secretory pathway and depends on both TMD length and signals in the cytoplasmic tail...
  42. pmc Defining the glycan destruction signal for endoplasmic reticulum-associated degradation
    Erin M Quan
    Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94143 2542, USA
    Mol Cell 32:870-7. 2008
    ..This strategy of marking potential substrates by Htm1p and decoding the signal by Yos9p is well suited to provide a proofreading mechanism that enhances substrate specificity...
  43. ncbi Modification of the N-glycosylation pathway to produce homogeneous, human-like glycans using GlycoSwitch plasmids
    Wouter Vervecken
    Department of Molecular Biomedical Research, Ghent University, Ghent, Belgium
    Methods Mol Biol 389:119-38. 2007
    ..Different plasmids are described as well as glycan analysis methods...
  44. pmc Cell type-dependent variations in the subcellular distribution of alpha-mannosidase I and II
    A Velasco
    Division of Cellular and Molecular Medicine and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093 0651
    J Cell Biol 122:39-51. 1993
    alpha-mannosidases I and II (Man I and II) are resident enzymes of the Golgi complex involved in oligosaccharide processing during N-linked glycoprotein biosynthesis that are widely considered to be markers of the cis- and medial-Golgi ..
  45. ncbi Characterization of a cDNA encoding a novel human Golgi alpha 1, 2-mannosidase (IC) involved in N-glycan biosynthesis
    L O Tremblay
    McGill Cancer Centre, McGill University, Montreal, Quebec H3G 1Y6, Canada
    J Biol Chem 275:31655-60. 2000
    A human cDNA encoding a 70.9-kDa type II membrane protein with sequence similarity to class I alpha1,2-mannosidases was isolated...
  46. pmc The mammalian UPR boosts glycoprotein ERAD by suppressing the proteolytic downregulation of ER mannosidase I
    Daniel J Termine
    Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA
    J Cell Sci 122:976-84. 2009
    ..A model is proposed in which ERManI, by functioning as a downstream effector target of EDEM1, represents a checkpoint activation paradigm by which the mammalian UPR coordinates the boosting of ERAD...
  47. ncbi N-Glycosylation affects endoplasmic reticulum degradation of a mutated derivative of carboxypeptidase yscY in yeast
    M Knop
    Institut fuer Biochemie, Universitaet Stuttgart, Germany
    Yeast 12:1229-38. 1996
    ..Our results suggest that the ER of yeast contains a glycosylation-dependent quality control system, as has been shown for higher eukaryotic cells...
  48. pmc Htm1 protein generates the N-glycan signal for glycoprotein degradation in the endoplasmic reticulum
    Simone Clerc
    Department of Biology, Institute of Microbiology, Eidgenossische Technische Hochschule Zurich, Zurich, Switzerland
    J Cell Biol 184:159-72. 2009
    ....
  49. ncbi Man9-mannosidase from human kidney is expressed in COS cells as a Golgi-resident type II transmembrane N-glycoprotein
    E Bieberich
    Institut fur Physiologische Chemie, Bonn, Germany
    Eur J Biochem 233:644-9. 1995
    ..This localization clearly differs from that of pig liver Man9-mannosidase which appears to be located in the endoplasmic reticulum and transient vesicles...
  50. pmc A germination-specific endo-beta-mannanase gene is expressed in the micropylar endosperm cap of tomato seeds
    H Nonogaki
    Department of Vegetable Crops, University of California, Davis, California 95616 8631, USA
    Plant Physiol 123:1235-46. 2000
    ..Distinct mannanases are involved in germination and post-germinative growth, with LeMAN2 being associated with endosperm cap weakening prior to radicle emergence, whereas LeMAN1 mobilizes galactomannan reserves in the lateral endosperm...
  51. ncbi Effects of macromolecular crowding on glycoprotein processing enzymes
    Kiichiro Totani
    RIKEN, The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Am Chem Soc 130:2101-7. 2008
    ..Our results indicate that the kinetics of glycan processing under crowded conditions may be quite different from those measured in dilute buffers...
  52. ncbi Substrate specificity and molecular cloning of the lily endo-beta-mannosidase acting on N-glycan
    Akiko Sasaki
    Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    J Biochem 137:87-93. 2005
    ..The enzyme is ubiquitously expressed, suggesting that it has a general function such as processing or degrading N-glycans...
  53. ncbi A Novel alpha1,2-L-fucosidase acting on xyloglucan oligosaccharides is associated with endo-beta-mannosidase
    Takeshi Ishimizu
    Department of Chemistry, Graduate School of Science, Osaka University, Osaka 560 0043, Japan
    J Biochem 142:721-9. 2007
    ..The amino acid sequence of this associated protein has no similarity to known plant alpha-L-fucosidases. These results show that EBM II is a novel alpha1,2-L-fucosidase and a protein complex containing endo-beta-mannosidase...
  54. pmc Single, context-specific glycans can target misfolded glycoproteins for ER-associated degradation
    Eric D Spear
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA
    J Cell Biol 169:73-82. 2005
    ..The discovery of a similar signal in misfolded proteinase A supported the generality of the mechanism. These studies show that specific signals embedded in glycoproteins can direct their degradation if they fail to fold...
  55. ncbi Structural diversity of cytosolic free oligosaccharides in the human hepatoma cell line, HepG2
    Kanta Yanagida
    Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Glycobiology 16:294-304. 2006
    ..M8A') by digestion with cytosolic alpha-mannosidase; series 2: oligosaccharides processed with Golgi alpha-mannosidases in addition to endoplasmic reticulum (ER) and cytosolic alpha-mannosidases; and series 3: glucosylated ..
  56. ncbi Stimulation of ERAD of misfolded null Hong Kong alpha1-antitrypsin by Golgi alpha1,2-mannosidases
    Nobuko Hosokawa
    Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8397, Saitama 332 0012, Japan
    Biochem Biophys Res Commun 362:626-32. 2007
    ..Although transfected NHK is primarily localized in the ER, some NHK also co-localizes with Golgi markers, suggesting that mannose trimming by Golgi alpha1,2-mannosidases can also contribute to NHK degradation.
  57. ncbi Synthesis of beta-mannosides using the transglycosylation activity of endo-beta-mannosidase from Lilium longiflorum
    Akiko Sasaki
    Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan
    FEBS J 272:1660-8. 2005
    ..Thus, the lily endo-beta-mannosidase is useful for the enzymatic preparation of oligosaccharides containing the mannosyl beta 1,4-structure, chemical preparations of which have been frequently reported to be difficult...
  58. pmc A novel endo-beta-mannanase gene in tomato LeMAN5 is associated with anther and pollen development
    Sergei A Filichkin
    Department of Horticulture, Oregon State University, Corvallis, Oregon 97331 7304, USA
    Plant Physiol 134:1080-7. 2004
    ..These data suggest that the LeMAN5 endo-beta-mannanase is associated with anther and pollen development...
  59. pmc Novel aspects of degradation of T cell receptor subunits from the endoplasmic reticulum (ER) in T cells: importance of oligosaccharide processing, ubiquitination, and proteasome-dependent removal from ER membranes
    M Yang
    Laboratory of Immune Cell Biology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 1152, USA
    J Exp Med 187:835-46. 1998
    ..However, no evidence of a role for mannosidases was found for TCR-alpha, and significant retrograde movement through the ER membrane took place even when ..
  60. ncbi Cyclic amidine sugars as transition-state analogue inhibitors of glycosidases: potent competitive inhibitors of mannosidases
    Marie Pierre Heck
    CEA, CE Saclay, Service de Marquage Moleculaire et de Chimie Bioorganique, Bat 547, Departement de Biologie Joliot Curie, 91191 Gif sur Yvette, France
    J Am Chem Soc 126:1971-9. 2004
    ..9b bearing an exocyclic ethylamine moiety proved to be a selective competitive inhibitor of alpha- and beta-mannosidases (K(i) = 6 nM) making it the most potent inhibitor of these glycosidases reported to date...
  61. ncbi Molecular cloning and characterization of Arabidopsis thaliana Golgi alpha-mannosidase II, a key enzyme in the formation of complex N-glycans in plants
    Richard Strasser
    Institute of Applied Genetics and Cell Biology, Vienna, Austria
    Plant J 45:789-803. 2006
    ..A GFP fusion construct containing only the transmembrane domain was predominantly retained in the ER, a result that indicates the presence of a motif promoting ER export within the last 10 amino acids of the cytoplasmic tail of AtGMII...
  62. ncbi Importance of carbohydrate positioning in the recognition of mutated CPY for ER-associated degradation
    Zlatka Kostova
    Institut fur Biochemie, Universitat Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart, Germany
    J Cell Sci 118:1485-92. 2005
    ..For cases in which both lectins are involved, Cne1p and Htm1p play competing roles in targeting the substrate for degradation: loss of Cne1p accelerates degradation, whereas loss of Htm1p stabilizes the substrate...
  63. ncbi Optimization of the production of thermostable endo-beta-1,4 mannanases from a newly isolated Aspergillus niger gr and Aspergillus flavus gr
    Naganagouda V Kote
    Department of Biochemistry, Gulbarga University, Karnataka, India
    Appl Biochem Biotechnol 152:213-23. 2009
    ..These new strains offer an attractive alternative source of enzymes for the food and feed processing industries...
  64. pmc Misfolded proteins are sorted by a sequential checkpoint mechanism of ER quality control
    Shilpa Vashist
    Department of Biochemistry and Molecular Biology, 408 South Frear Laboratory, Pennsylvania State University, University Park, PA 16802, USA
    J Cell Biol 165:41-52. 2004
    ..Our data support a model whereby "properly folded" proteins are defined biologically as survivors that endure a series of distinct checkpoints...
  65. ncbi Cloning, expression and characterization of endo-beta-1,4-mannanase from Aspergillus fumigatus in Aspergillus sojae and Pichia pastoris
    Gökhan Duruksu
    Dept of Biotechnology, Middle East Technical University, Ankara, Turkey
    Biotechnol Prog 25:271-6. 2009
    ..5 and 5.2 for A. sojae and P. pastoris, respectively. The enzyme from P. pastoris was more stable retaining most of the activity up to 50 degrees C, whereas the enzyme from A. sojae rapidly lost activity above 40 degrees C...
  66. ncbi Comparison of kifunensine and 1-deoxymannojirimycin binding to class I and II alpha-mannosidases demonstrates different saccharide distortions in inverting and retaining catalytic mechanisms
    Niket Shah
    Department of Medical Biophysics, University of Toronto, Ontario, Canada
    Biochemistry 42:13812-6. 2003
    b>Mannosidases are key enzymes in the eukaryotic N-glycosylation pathway. These enzymes fall into two broad classes (I and II) and are characteristically different in catalytic mechanism, sequence, and structure...
  67. ncbi Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line
    Francois Foulquier
    Unite de Glycobiologie Structurale et Fonctionnelle, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Eur J Biochem 271:398-404. 2004
    ....
  68. ncbi Heterologous expression and optimized production of an Aspergillus aculeatus endo-1,4-beta-mannanase in Yarrowia lipolytica
    Robyn Roth
    CSIR Biosciences, Council of Scientific and Industrial Research, Pretoria 0001, South Africa
    Mol Biotechnol 43:112-20. 2009
    ....
  69. pmc Processing of N-linked glycans during endoplasmic-reticulum-associated degradation of a short-lived variant of ribophorin I
    Claudia Kitzmuller
    Max F Perutz Laboratories, University Departments at the Vienna Biocenter and Institute of Medical Biochemistry, Department of Molecular Genetics, University of Vienna, Austria
    Biochem J 376:687-96. 2003
    ..N-glycan species, in particular Glc(1)Man(7)GlcNAc(2), were also found on the protein, indicating that several mannosidases might be implicated in the initial trimming of the oligomannoside...
  70. ncbi N-glycan structure of a short-lived variant of ribophorin I expressed in the MadIA214 glycosylation-defective cell line reveals the role of a mannosidase that is not ER mannosidase I in the process of glycoprotein degradation
    M Ermonval
    URA CNRS 1960, , 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Glycobiology 11:565-76. 2001
    ..Our results unambiguously demonstrate that an alpha1,2-mannosidase that is not ER mannosidase I is involved in ERAD of RI(332-)3HA in the glycosylation-defective cell line, MadIA214...
  71. pmc Htm1p, a mannosidase-like protein, is involved in glycoprotein degradation in yeast
    C A Jakob
    Institute of Microbiology, ETH Zurich, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
    EMBO Rep 2:423-30. 2001
    ..We propose that this mannosidase homolog is a lectin that recognizes Man8GlcNAc2 oligosaccharides that serve as signals in the degradation pathway...
  72. ncbi Cloning and expression in Pichia pastoris of a blue mussel (Mytilus edulis) beta-mannanase gene
    Bingze Xu
    Center for Surface Biotechnology, Uppsala Biomedical Center, Uppsala University, Sweden
    Eur J Biochem 269:1753-60. 2002
    ..In conclusion, P. pastoris is regarded as a suitable host strain for the production of blue mussel beta-mannanase. This is the first time a mollusc beta-mannanase has been characterized at the DNA level...
  73. ncbi A highly thermostable endo-(1,4)-beta-mannanase from the marine bacterium Rhodothermus marinus
    O Politz
    Department of Dermatology, Benjamin Franklin Medical Centre, The Free University of Berlin, Germany
    Appl Microbiol Biotechnol 53:715-21. 2000
    ..In contrast, R. marinus-derived protein retained 87% activity after 1 h at 90 degrees C. The enzyme hydrolysed carob-galactomannan (locust bean gum) effectively and to a smaller extent guar gum, but not yeast mannan...
  74. ncbi Mnl1p, an alpha -mannosidase-like protein in yeast Saccharomyces cerevisiae, is required for endoplasmic reticulum-associated degradation of glycoproteins
    K Nakatsukasa
    Department of Chemistry, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    J Biol Chem 276:8635-8. 2001
    ..Possible roles of Mnl1p in the degradation and in the ER-retention of misfolded glycoproteins are discussed...
  75. ncbi Structure and function of Class I alpha 1,2-mannosidases involved in glycoprotein synthesis and endoplasmic reticulum quality control
    A Herscovics
    McGill Cancer Centre, McGill University, 3655 Promenade Sir William Osler, Montreal, H3G 1Y6, Quebec, Canada
    Biochimie 83:757-62. 2001
    Class I alpha 1,2-mannosidases (glycosylhydrolase family 47) are conserved through eukaryotic evolution. This protein family comprises three subgroups distinguished by their enzymatic properties...
  76. ncbi Structural insights into the beta-mannosidase from T. reesei obtained by synchrotron small-angle X-ray solution scattering enhanced by X-ray crystallography
    Ricardo Aparicio
    Instituto de Fisica Gleb Wataghin, Universidade Estadual de Campinas, Campinas, SP, Brazil
    Biochemistry 41:9370-5. 2002
    ..The method could be applied as a general method in structural genomics and related fields once protein solution scattering data are available...
  77. ncbi endo-beta-1,4-Mannanases from blue mussel, Mytilus edulis: purification, characterization, and mode of action
    Bingze Xu
    Center for Surface Biotechnology, Biomedical Center, Uppsala University, Box 577, SE 751 23 Uppsala, Sweden
    J Biotechnol 92:267-77. 2002
    ..The enzymes readily degrade the galactomannans from locust bean gum and ivory nut mannan but show no cross-specificity for xylan and carboxymethyl cellulose. There is no binding ability observed towards cellulose and mannan...
  78. pmc Morphological analysis of protein transport from the ER to Golgi membranes in digitonin-permeabilized cells: role of the P58 containing compartment
    H Plutner
    Department of Cell and Molecular Biology, Scripps Research Institute, La Jolla, California 92037
    J Cell Biol 119:1097-116. 1992
    ....
  79. ncbi A cellulose-binding module of the Trichoderma reesei beta-mannanase Man5A increases the mannan-hydrolysis of complex substrates
    Per Hägglund
    Department of Biochemistry, Centre for Chemistry and Chemical Engineering, Lund University, PO Box 124, S 221 00 Lund, Sweden
    J Biotechnol 101:37-48. 2003
    ..Moreover, the binding of Man5A to cellulose was compared with that of an endoglucanase (Cel7B) from T. reesei...
  80. ncbi Two homologous genes, DCW1 (YKL046c) and DFG5, are essential for cell growth and encode glycosylphosphatidylinositol (GPI)-anchored membrane proteins required for cell wall biogenesis in Saccharomyces cerevisiae
    Hiroshi Kitagaki
    National Research Institute of Brewing, Higashihiroshima, Japan
    Mol Microbiol 46:1011-22. 2002
    ..These results suggest that both Dcw1p and Dfg5p are GPI-anchored membrane proteins and are required for normal biosynthesis of the cell wall...
  81. ncbi Molecular and biochemical characterization of endo-beta-mannanases from germinating coffee (Coffea arabica) grains
    P Marraccini
    Department of Plant Science, Nestle Research Centre, Tours, France
    Planta 213:296-308. 2001
    ..Activity and mRNA levels appeared to be tightly co-ordinated. Tests of substrate specificity with the purified ManB enzyme showed that activity required a minimum of five mannose units to function efficiently...
  82. ncbi Role of N-glycan-dependent quality control in the cell-surface expression of the AT1 receptor
    Pascal M Lanctot
    Department of Pharmacology, Faculty of Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, Que, Canada J1H 5N4
    Biochem Biophys Res Commun 340:395-402. 2006
    ....
  83. ncbi The synthesis of a series of modified mannotrisaccharides as probes of the enzymes involved in the early stages of mammalian complex N-glycan formation
    Chris A Tarling
    Department of Chemistry, 2036 Main Mall, University of British Columbia, Vancouver, BC, Canada V6T 1Z1
    Carbohydr Res 339:2487-97. 2004
    ..Secondly it should render trisaccharide 8 stable to hydrolysis by the mannosidases Man-II and Man-III by inductive destabilization of their oxocarbenium ion-like transition states...
  84. ncbi Enzymatic glycosylation of lincomycin
    L Weignerova
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague
    Biosci Biotechnol Biochem 65:1897-9. 2001
    ..Compound 1 was glycosylated using jack bean alpha-mannosidase to produce 7-O-alpha-D-mannopyranosyl-lincomycin (2)...
  85. ncbi Immunocytochemical localization of the Menkes copper transport protein (ATP7A) to the trans-Golgi network
    H A Dierick
    Department of Pediatrics, University of Michigan, Ann Arbor 48109 0618, USA
    Hum Mol Genet 6:409-16. 1997
    ....
  86. ncbi The roles of enzyme localisation and complex formation in glycan assembly within the Golgi apparatus
    Christopher L de Graffenried
    Department of Chemistry, University of California, Berkeley, California 94720 1460, USA
    Curr Opin Cell Biol 16:356-63. 2004
    ..Together, localisation and association govern the assembly of complex glycans and thereby regulate interactions at the cell surface...
  87. ncbi Overexpression of the Golgi-localized enzyme alpha-mannosidase IIx in Chinese hamster ovary cells results in the conversion of hexamannosyl-N-acetylchitobiose to tetramannosyl-N-acetylchitobiose in the N-glycan-processing pathway
    M Oh-Eda
    Glycobiology Program, The Burnham Institute, La Jolla, CA 92037, USA
    Eur J Biochem 268:1280-8. 2001
    ....
  88. ncbi Isolation of a mouse Golgi mannosidase cDNA, a member of a gene family conserved from yeast to mammals
    A Herscovics
    McGill Cancer Centre, McGill University, Montreal, Quebec, Canada
    J Biol Chem 269:9864-71. 1994
    ..the deduced amino acid sequence of a rabbit liver alpha-mannosidase partial cDNA, demonstrating that processing mannosidases have been conserved through eukaryotic evolution...
  89. ncbi Transient calnexin interaction confers long-term stability on folded K+ channel protein in the ER
    Rajesh Khanna
    Department of Physiology and Molecular Biology Institute, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095 1751, USA
    J Cell Sci 117:2897-908. 2004
    ..Our results indicate that transient interaction with calnexin provides long-term protection from ER-associated degradation...
  90. ncbi Intestinal dipeptidyl peptidase IV is efficiently sorted to the apical membrane through the concerted action of N- and O-glycans as well as association with lipid microdomains
    Marwan Alfalah
    Department of Physiological Chemistry, School of Veterinary Medicine Hannover, Hannover D 30559, Germany
    J Biol Chem 277:10683-90. 2002
    ....
  91. ncbi A novel Rab GTPase, Rab33B, is ubiquitously expressed and localized to the medial Golgi cisternae
    J Y Zheng
    Section of Bacterial Infection, Institute of Immunological Science, Hokkaido University, Sapporo, Japan
    J Cell Sci 111:1061-9. 1998
    ..Immunoelectron microscopy analysis further defined the localization of Rab33B to the medial Golgi cisternae. These results suggest Rab33B plays a role in intra-Golgi transport...
  92. ncbi A novel stress-induced EDEM variant regulating endoplasmic reticulum-associated glycoprotein degradation
    Silvia Olivari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    J Biol Chem 280:2424-8. 2005
    ....
  93. ncbi The involvement of mnn4 and mnn6 mutations in mannosylphosphorylation of O-linked oligosaccharide in yeast Saccharomyces cerevisiae
    K Nakayama
    Molecular Biology Department, National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, Japan
    Biochim Biophys Acta 1425:255-62. 1998
    ..The amount of mannosylphosphorylated mannotriose was 7% of total O-linked oligosaccharides (20% of neutral mannotriose) of chitinase in strain mnn1...
  94. ncbi The structure of bovine lysosomal alpha-mannosidase suggests a novel mechanism for low-pH activation
    Pirkko Heikinheimo
    Institutt for Kjemi, Universitetet i Tromsø, N 9037, Tromsø, Norway
    J Mol Biol 327:631-44. 2003
    ..Furthermore, it provides a basis for understanding the human form of alpha-mannosidosis at the atomic level. The atomic coordinates and structure factors have been deposited in the Protein Data Bank (accession codes 1o7d and r1o7dsf)...
  95. ncbi Matrix proteins can generate the higher order architecture of the Golgi apparatus
    J Seemann
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520 8002, USA
    Nature 407:1022-6. 2000
    ..This new concept of the Golgi further indicates that the Golgi may be an autonomous organelle rather than one that is in simple dynamic equilibrium with the endoplasmic reticulum...
  96. ncbi Endomannosidase processes oligosaccharides of alpha1-antitrypsin and its naturally occurring genetic variants in the Golgi apparatus
    T Torossi
    Department of Pathology, Division of Cell and Molecular Pathology, University of Zurich, Schmelzbergstrasse 12, 8091, Zurich, Switzerland
    Cell Mol Life Sci 63:1923-32. 2006
    ..For exported misfolded glycoproteins, this would provide a pathway for the formation of mature oligosaccharides important for their proper trafficking and correct functioning...
  97. ncbi SS33410, an inhibitor of V-ATPase, blocks intracellular protein transport of the VSV-G protein in the Golgi compartment
    Dae Hyun Seog
    Department of Microbiology, College of Medicine, Inje University, Busan, Korea
    Biosci Biotechnol Biochem 67:2591-7. 2003
    ..These studies thus suggest that SS33410 blocks the intracellular protein transport before the step of trimming by mannosidase II, which is confined to the medial Golgi compartment...
  98. ncbi Synthesis, enzymatic activity, and X-ray crystallography of an unusual class of amino acids
    Wang Chen
    Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada V5A 1S6
    Bioorg Med Chem 14:8332-40. 2006
    ..The same compound inhibits recombinant human maltase glucoamylase, one of the key intestinal enzymes involved in the breakdown of glucose oligosaccharides in the small intestine, with a K(i) value of 21microM...
  99. pmc Steady-state localization of a medial-Golgi glycosyltransferase involves transit through the trans-Golgi network
    A S Opat
    Department of Pathology and Immunology, Monash University Medical School, Commercial Road, Prahran, Melbourne, Victoria 3181, Australia
    Biochem J 358:33-40. 2001
    ..Furthermore, this data suggests that retrograde transport pathways play an important role in establishing the asymmetric distribution of GlcNAc-TI within the Golgi stack...
  100. ncbi Cloning, expression, purification, and characterization of the acid alpha-mannosidase from Trypanosoma cruzi
    A S Vandersall-Nairn
    Complex Carbohydrate Research Center and the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
    Glycobiology 8:1183-94. 1998
    ..A polyclonal antibody raised to the recombinant enzyme was shown to immunoprecipitate the alpha-mannosidase from T.cruzi cell extracts and will be used in future immunolocalization studies...
  101. ncbi A combined STD-NMR/molecular modeling protocol for predicting the binding modes of the glycosidase inhibitors kifunensine and salacinol to Golgi alpha-mannosidase II
    Xin Wen
    Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6
    Biochemistry 44:6729-37. 2005
    ....

Research Grants17

  1. MOLECULAR STUDIES OF RETINAL DEGENERATION IN DROSOPHILA
    Nansi J Colley; Fiscal Year: 2013
    ..role in vesicular transport of rhodopsin and other constituents of phototransduction, and (2) the family of mannosidases and their key roles in trimming carbohydrates in the ER and Golgi during rhodopsin biosynthesis...
  2. Mannosidase inhibitors as therapeutics for glycoprotein misfolding diseases
    Kelley W Moremen; Fiscal Year: 2010
    ..They also inhibit glycan processing alpha-mannosidases in the Golgi complex and block maturation to complex type glycan structures on cell surface and secreted ..
  3. CARHOHYDRATE MOIETIES OF GP120
    Khushi Matta; Fiscal Year: 1993
    ..studies toward the chemical synthesis of various potential inhibitors of glycosidases, such as glucosidases and mannosidases, involved in the processing and biosynthesis of glycoprotein including gp120...
  4. NOVEL GLYCOSIDASE INHIBITORS AS ANTICANCER AGENTS
    BRIAN COPPOLA; Fiscal Year: 2002
    ..Second, SW causes an undesired inhibition of lysosomal alpha- mannosidases, which induces a reversible phenocopy of the genetic lysosomal storage disease alpha-mannosidosis...
  5. Selective Mannosidase Inhibitors as Cancer Therapeutics
    Kelley Moremen; Fiscal Year: 2004
    ..Significant differences in the substrate binding specificities of these two mannosidases can now be exploited to develop an inhibitor that is selective for Golgi alpha-mannosidase II...
  6. MANNOSE IN MAMMALIAN GLYCOPROTEIN SYNTHESIS
    Hudson Freeze; Fiscal Year: 2001
    ..4. Demonstrate glycoprotein digestion and alpha-mannosidases responsible for it...
  7. Induction of 2G12 neutralizing antibody by plant-derived HIVgp145
    Yvonne Rosenberg; Fiscal Year: 2007
    ..of HIV gp145 to the endoplasmic reticulum using a KDEL tag which blocks exit into the Golgi and exposure to mannosidases. These high-mannose HIV Env molecules exhibited stronger reactivity with 2G12 than CHO-derived Env, suggesting ..
  8. Mannosidases in Glycoprotein Biosynthesis & Catabolism
    Kelley Moremen; Fiscal Year: 2008
    ..We have focused on the Class I (family 47) a-mannosidases in the N-glycan pathway since they act as committed steps in the synthesis of complex oligosaccharides by ..
  9. PATHOBIOGENESIS OF HERITABLE PULMONARY EMPHYSEMA
    Richard Sifers; Fiscal Year: 2003
    ..The hypothesis to be tested is that modification by intracellular mannosidases sorts genetic variant PIZ for non-proteasomal elimination by promoting specific sequential interactions with ..
  10. EXPRESSION AND IN VIVO ROLE OF TROPHININ IN MICE
    Michiko Fukuda; Fiscal Year: 2002
    ....
  11. Substrate specific ERK docking domain inhibitors
    Paul S Shapiro; Fiscal Year: 2010
    ..These compounds will be appropriate for research tools and the development into novel chemotherapeutic agents aimed at treating cancers associated with elevated ERK pathway activity. ..
  12. Post-translational Surveillance of Genome Expression
    Richard Sifers; Fiscal Year: 2009
    ..Moreover, the study will have broad implications, and serve as a paradigm, for the investigation of numerous protein-folding diseases. ..
  13. Multi-ethnic Genome-wide Alzheimer association study
    Lindsay A Farrer; Fiscal Year: 2010
    ....
  14. Development of ERK docking domain inhibitors
    Paul Shapiro; Fiscal Year: 2005
    ..These studies represent a novel approach for developing compounds that selectively block ERK activation of proteins involved in cancer cell proliferation. ..