mannosidases

Summary

Summary: Glycoside hydrolases that catalyze the hydrolysis of alpha or beta linked MANNOSE.

Top Publications

  1. pmc A novel ER alpha-mannosidase-like protein accelerates ER-associated degradation
    N Hosokawa
    Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8397, Japan
    EMBO Rep 2:415-22. 2001
  2. doi Effects of macromolecular crowding on glycoprotein processing enzymes
    Kiichiro Totani
    RIKEN, The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Am Chem Soc 130:2101-7. 2008
  3. pmc Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans
    Yunkyoung Song
    School of Bioscience and Biotechnology, Chungnam National University, Daejeon 305 764, Korea
    Appl Environ Microbiol 73:4446-54. 2007
  4. ncbi Functional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastoris
    Robert C Davidson
    GlycoFi, Inc, 21 Lafayette Street Suite 200, Lebanon, NH 03766 Velocity 11 435 Acacia Ave, Palo Alto, CA 94306, USA
    Glycobiology 14:399-407. 2004
  5. pmc Plant N-glycan processing enzymes employ different targeting mechanisms for their spatial arrangement along the secretory pathway
    Claude Saint-Jore-Dupas
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6037, IFRMP 23, GDR 2590, UFR des Sciences, Universite de Rouen, 76821 Mont Saint Aignan Cedex, France
    Plant Cell 18:3182-200. 2006
  6. ncbi Use of HDEL-tagged Trichoderma reesei mannosyl oligosaccharide 1,2-alpha-D-mannosidase for N-glycan engineering in Pichia pastoris
    N Callewaert
    Unit of Fundamental and Applied Molecular Biology, Department of Molecular Biology, Ghent University, Ghent, Belgium
    FEBS Lett 503:173-8. 2001
  7. pmc Disruption of the processing alpha-mannosidase gene does not prevent outer chain synthesis in Saccharomyces cerevisiae
    R Puccia
    McGill Cancer Centre, McGill University, Montreal, Quebec, Canada
    Biochem J 290:21-6. 1993
  8. ncbi Molecular cloning of a cDNA encoding a (1-->4)-beta-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum)
    J D Bewley
    Department of Botany, University of Guelph, Ontario, Canada
    Planta 203:454-9. 1997
  9. ncbi Molecular cloning, chromosomal mapping and tissue-specific expression of a novel human alpha1,2-mannosidase gene involved in N-glycan maturation
    L O Tremblay
    McGill Cancer Centre, McGill University, 3655 Drummond Street, Montreal, Quebec, Canada H3G 1Y6
    Glycobiology 8:585-95. 1998
  10. ncbi Molecular cloning and primary structure of Man9-mannosidase from human kidney
    E Bause
    Institut fur Physiologische Chemie, Universitat Bonn, Germany
    Eur J Biochem 217:535-40. 1993

Research Grants

  1. ALPHA-MANNOSIDASES IN MAMMALIAN GLYCOPROTEIN PROCESSING
    Kelley Moremen; Fiscal Year: 1993
  2. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2000
  3. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 1999
  4. CARHOHYDRATE MOIETIES OF GP120
    Khushi Matta; Fiscal Year: 1993
  5. CARHOHYDRATE MOIETIES OF GP120
    Khushi Matta; Fiscal Year: 1991
  6. ALPHA-MANNOSIDASES IN MAMMALIAN GLYCOPROTEIN PROCESSING
    Kelley Moremen; Fiscal Year: 1992
  7. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2003
  8. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2001
  9. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2005
  10. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2004

Detail Information

Publications172 found, 100 shown here

  1. pmc A novel ER alpha-mannosidase-like protein accelerates ER-associated degradation
    N Hosokawa
    Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8397, Japan
    EMBO Rep 2:415-22. 2001
    ..luminal region of ER degradation enhancing alpha-mannosidase-like protein (EDEM) is similar to class I alpha1,2-mannosidases involved in N-glycan processing, EDEM did not have enzymatic activity...
  2. doi Effects of macromolecular crowding on glycoprotein processing enzymes
    Kiichiro Totani
    RIKEN, The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Am Chem Soc 130:2101-7. 2008
    ..Our results indicate that the kinetics of glycan processing under crowded conditions may be quite different from those measured in dilute buffers...
  3. pmc Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans
    Yunkyoung Song
    School of Bioscience and Biotechnology, Chungnam National University, Daejeon 305 764, Korea
    Appl Environ Microbiol 73:4446-54. 2007
    ..Therefore, the Yloch1 mutant strain can be used as a host to produce glycoproteins lacking the outer-chain mannoses and further developed for the production of therapeutic glycoproteins containing human-compatible oligosaccharides...
  4. ncbi Functional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastoris
    Robert C Davidson
    GlycoFi, Inc, 21 Lafayette Street Suite 200, Lebanon, NH 03766 Velocity 11 435 Acacia Ave, Palo Alto, CA 94306, USA
    Glycobiology 14:399-407. 2004
    ..pastoris alg3 deletion mutant in the presence and absence of the outer chain initiating Och1p alpha-1,6-mannosyltransferase activity suggests that the PpOch1p has a broader substrate specificity compared to its S. cerevisiae counterpart...
  5. pmc Plant N-glycan processing enzymes employ different targeting mechanisms for their spatial arrangement along the secretory pathway
    Claude Saint-Jore-Dupas
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6037, IFRMP 23, GDR 2590, UFR des Sciences, Universite de Rouen, 76821 Mont Saint Aignan Cedex, France
    Plant Cell 18:3182-200. 2006
    ..We conclude that the localization of N-glycan processing enzymes corresponds to an assembly line in the early secretory pathway and depends on both TMD length and signals in the cytoplasmic tail...
  6. ncbi Use of HDEL-tagged Trichoderma reesei mannosyl oligosaccharide 1,2-alpha-D-mannosidase for N-glycan engineering in Pichia pastoris
    N Callewaert
    Unit of Fundamental and Applied Molecular Biology, Department of Molecular Biology, Ghent University, Ghent, Belgium
    FEBS Lett 503:173-8. 2001
    ..Moreover, the human-type high-mannose oligosaccharide Man(5)GlcNAc(2) was the major N-glycan of the glyco-engineered trans-sialidase, indicating that N-glycan engineering can be effectively accomplished in P. pastoris...
  7. pmc Disruption of the processing alpha-mannosidase gene does not prevent outer chain synthesis in Saccharomyces cerevisiae
    R Puccia
    McGill Cancer Centre, McGill University, Montreal, Quebec, Canada
    Biochem J 290:21-6. 1993
    ..It is concluded that the alpha-mannosidase encoded by MNS1 is the only enzyme responsible for mannose removal in vivo, and that this processing step is not essential for outer chain synthesis...
  8. ncbi Molecular cloning of a cDNA encoding a (1-->4)-beta-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum)
    J D Bewley
    Department of Botany, University of Guelph, Ontario, Canada
    Planta 203:454-9. 1997
    ..Expression of the genes, as determined by the presence of mRNA transcripts in Northern hybridization analyses, occurs in the endosperm of germinated seeds; no transcripts are detected in hypocotyls, cotyledons, roots or leaves...
  9. ncbi Molecular cloning, chromosomal mapping and tissue-specific expression of a novel human alpha1,2-mannosidase gene involved in N-glycan maturation
    L O Tremblay
    McGill Cancer Centre, McGill University, 3655 Drummond Street, Montreal, Quebec, Canada H3G 1Y6
    Glycobiology 8:585-95. 1998
    Class I alpha1,2-mannosidases play an essential role in the elaboration of complex and hybrid N -glycans in mammalian cells...
  10. ncbi Molecular cloning and primary structure of Man9-mannosidase from human kidney
    E Bause
    Institut fur Physiologische Chemie, Universitat Bonn, Germany
    Eur J Biochem 217:535-40. 1993
    ..This finding may indicate that the mammalian alpha-mannosidases, which differ significantly in their substrate specificity, are coded for by evolutionarily unrelated genes, ..
  11. ncbi Endo-beta-mannanase activity increases in the skin and outer pericarp of tomato fruits during ripening
    J D Bewley
    Department of Botany, University of Guelph, Ontario, Canada
    J Exp Bot 51:529-38. 2000
    ....
  12. ncbi Enhancement of endoplasmic reticulum (ER) degradation of misfolded Null Hong Kong alpha1-antitrypsin by human ER mannosidase I
    Nobuko Hosokawa
    Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8397, Japan
    J Biol Chem 278:26287-94. 2003
    ....
  13. pmc Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-beta-mannosidase from Aspergillus niger BK01
    Bien Cuong Do
    School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Nakhon Ratchasima 30000, Thailand
    Microb Cell Fact 8:59. 2009
    ..Mannan endo-1,4-beta-mannosidases (1,4-beta-D-mannanases) catalyze the random hydrolysis of beta-1,4-mannosidic linkages in the main chain of ..
  14. ncbi Substrate specificity and molecular cloning of the lily endo-beta-mannosidase acting on N-glycan
    Akiko Sasaki
    Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    J Biochem 137:87-93. 2005
    ..The enzyme is ubiquitously expressed, suggesting that it has a general function such as processing or degrading N-glycans...
  15. ncbi Structural diversity of cytosolic free oligosaccharides in the human hepatoma cell line, HepG2
    Kanta Yanagida
    Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Glycobiology 16:294-304. 2006
    ..M8A') by digestion with cytosolic alpha-mannosidase; series 2: oligosaccharides processed with Golgi alpha-mannosidases in addition to endoplasmic reticulum (ER) and cytosolic alpha-mannosidases; and series 3: glucosylated ..
  16. pmc In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris
    Wouter Vervecken
    Department of Molecular Biomedical Research, Ghent University and Flanders Interuniversity Institute for Biotechnology, Ghent, Belgium
    Appl Environ Microbiol 70:2639-46. 2004
    ..The procedures which we developed allow glycan engineering of any P. pastoris expression strain and can yield up to 90% homogeneous protein-linked oligosaccharides...
  17. pmc Single, context-specific glycans can target misfolded glycoproteins for ER-associated degradation
    Eric D Spear
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA
    J Cell Biol 169:73-82. 2005
    ..The discovery of a similar signal in misfolded proteinase A supported the generality of the mechanism. These studies show that specific signals embedded in glycoproteins can direct their degradation if they fail to fold...
  18. ncbi A Novel alpha1,2-L-fucosidase acting on xyloglucan oligosaccharides is associated with endo-beta-mannosidase
    Takeshi Ishimizu
    Department of Chemistry, Graduate School of Science, Osaka University, Osaka 560 0043, Japan
    J Biochem 142:721-9. 2007
    ..The amino acid sequence of this associated protein has no similarity to known plant alpha-L-fucosidases. These results show that EBM II is a novel alpha1,2-L-fucosidase and a protein complex containing endo-beta-mannosidase...
  19. pmc Variation in its C-terminal amino acids determines whether endo-beta-mannanase is active or inactive in ripening tomato fruits of different cultivars
    Richard Bourgault
    Department of Botany, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    Plant Physiol 130:1254-62. 2002
    ..Site-directed mutagenesis was used to establish key elements in the C terminus of the endo-beta-mannanase protein that are essential for full enzyme activity...
  20. ncbi Stimulation of ERAD of misfolded null Hong Kong alpha1-antitrypsin by Golgi alpha1,2-mannosidases
    Nobuko Hosokawa
    Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8397, Saitama 332 0012, Japan
    Biochem Biophys Res Commun 362:626-32. 2007
    ..Although transfected NHK is primarily localized in the ER, some NHK also co-localizes with Golgi markers, suggesting that mannose trimming by Golgi alpha1,2-mannosidases can also contribute to NHK degradation.
  21. ncbi Endo-beta-mannosidase, a plant enzyme acting on N-glycan: purification, molecular cloning, and characterization
    Takeshi Ishimizu
    Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    J Biol Chem 279:38555-62. 2004
    ..acid sequence of AtEBM has distinct differences from those of the bacterial, fungal, and animal exo-type beta-mannosidases. Indeed, AtEBM-like genes are only found in plants, indicating that endo-beta-mannosidase is a plant-specific ..
  22. pmc Degradation of misfolded endoplasmic reticulum glycoproteins in Saccharomyces cerevisiae is determined by a specific oligosaccharide structure
    C A Jakob
    Division of Cell and Molecular Pathology, Department of Pathology, University of Zurich, CH 8091 Zurich, Switzerland
    J Cell Biol 142:1223-33. 1998
    ..These results suggest a role for the Man8GlcNAc2 oligosaccharide in the degradation process. They may indicate the presence of a Man8GlcNAc2-binding lectin involved in targeting of misfolded glycoproteins to degradation in S. cerevisiae...
  23. ncbi Synthesis of beta-mannosides using the transglycosylation activity of endo-beta-mannosidase from Lilium longiflorum
    Akiko Sasaki
    Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan
    FEBS J 272:1660-8. 2005
    ..Thus, the lily endo-beta-mannosidase is useful for the enzymatic preparation of oligosaccharides containing the mannosyl beta 1,4-structure, chemical preparations of which have been frequently reported to be difficult...
  24. pmc Novel aspects of degradation of T cell receptor subunits from the endoplasmic reticulum (ER) in T cells: importance of oligosaccharide processing, ubiquitination, and proteasome-dependent removal from ER membranes
    M Yang
    Laboratory of Immune Cell Biology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 1152, USA
    J Exp Med 187:835-46. 1998
    ..However, no evidence of a role for mannosidases was found for TCR-alpha, and significant retrograde movement through the ER membrane took place even when ..
  25. pmc A novel endo-beta-mannanase gene in tomato LeMAN5 is associated with anther and pollen development
    Sergei A Filichkin
    Department of Horticulture, Oregon State University, Corvallis, Oregon 97331 7304, USA
    Plant Physiol 134:1080-7. 2004
    ..These data suggest that the LeMAN5 endo-beta-mannanase is associated with anther and pollen development...
  26. ncbi endo-beta-1,4-Mannanases from blue mussel, Mytilus edulis: purification, characterization, and mode of action
    Bingze Xu
    Center for Surface Biotechnology, Biomedical Center, Uppsala University, Box 577, SE 751 23 Uppsala, Sweden
    J Biotechnol 92:267-77. 2002
    ..The enzymes readily degrade the galactomannans from locust bean gum and ivory nut mannan but show no cross-specificity for xylan and carboxymethyl cellulose. There is no binding ability observed towards cellulose and mannan...
  27. pmc Htm1p, a mannosidase-like protein, is involved in glycoprotein degradation in yeast
    C A Jakob
    Institute of Microbiology, ETH Zurich, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
    EMBO Rep 2:423-30. 2001
    ..We propose that this mannosidase homolog is a lectin that recognizes Man8GlcNAc2 oligosaccharides that serve as signals in the degradation pathway...
  28. ncbi Cloning and expression in Pichia pastoris of a blue mussel (Mytilus edulis) beta-mannanase gene
    Bingze Xu
    Center for Surface Biotechnology, Uppsala Biomedical Center, Uppsala University, Sweden
    Eur J Biochem 269:1753-60. 2002
    ..In conclusion, P. pastoris is regarded as a suitable host strain for the production of blue mussel beta-mannanase. This is the first time a mollusc beta-mannanase has been characterized at the DNA level...
  29. ncbi Structural insights into the beta-mannosidase from T. reesei obtained by synchrotron small-angle X-ray solution scattering enhanced by X-ray crystallography
    Ricardo Aparicio
    Instituto de Fisica Gleb Wataghin, Universidade Estadual de Campinas, Campinas, SP, Brazil
    Biochemistry 41:9370-5. 2002
    ..The method could be applied as a general method in structural genomics and related fields once protein solution scattering data are available...
  30. ncbi Mnl1p, an alpha -mannosidase-like protein in yeast Saccharomyces cerevisiae, is required for endoplasmic reticulum-associated degradation of glycoproteins
    K Nakatsukasa
    Department of Chemistry, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    J Biol Chem 276:8635-8. 2001
    ..Possible roles of Mnl1p in the degradation and in the ER-retention of misfolded glycoproteins are discussed...
  31. ncbi Structure and function of Class I alpha 1,2-mannosidases involved in glycoprotein synthesis and endoplasmic reticulum quality control
    A Herscovics
    McGill Cancer Centre, McGill University, 3655 Promenade Sir William Osler, Montreal, H3G 1Y6, Quebec, Canada
    Biochimie 83:757-62. 2001
    Class I alpha 1,2-mannosidases (glycosylhydrolase family 47) are conserved through eukaryotic evolution. This protein family comprises three subgroups distinguished by their enzymatic properties...
  32. ncbi N-glycan structure of a short-lived variant of ribophorin I expressed in the MadIA214 glycosylation-defective cell line reveals the role of a mannosidase that is not ER mannosidase I in the process of glycoprotein degradation
    M Ermonval
    URA CNRS 1960, , 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Glycobiology 11:565-76. 2001
    ..Our results unambiguously demonstrate that an alpha1,2-mannosidase that is not ER mannosidase I is involved in ERAD of RI(332-)3HA in the glycosylation-defective cell line, MadIA214...
  33. ncbi Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line
    Francois Foulquier
    Unite de Glycobiologie Structurale et Fonctionnelle, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Eur J Biochem 271:398-404. 2004
    ....
  34. pmc Misfolded proteins are sorted by a sequential checkpoint mechanism of ER quality control
    Shilpa Vashist
    Department of Biochemistry and Molecular Biology, 408 South Frear Laboratory, Pennsylvania State University, University Park, PA 16802, USA
    J Cell Biol 165:41-52. 2004
    ..Our data support a model whereby "properly folded" proteins are defined biologically as survivors that endure a series of distinct checkpoints...
  35. ncbi Importance of carbohydrate positioning in the recognition of mutated CPY for ER-associated degradation
    Zlatka Kostova
    Institut fur Biochemie, Universitat Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart, Germany
    J Cell Sci 118:1485-92. 2005
    ..For cases in which both lectins are involved, Cne1p and Htm1p play competing roles in targeting the substrate for degradation: loss of Cne1p accelerates degradation, whereas loss of Htm1p stabilizes the substrate...
  36. doi Heterologous expression and optimized production of an Aspergillus aculeatus endo-1,4-beta-mannanase in Yarrowia lipolytica
    Robyn Roth
    CSIR Biosciences, Council of Scientific and Industrial Research, Pretoria 0001, South Africa
    Mol Biotechnol 43:112-20. 2009
    ....
  37. pmc Morphological analysis of protein transport from the ER to Golgi membranes in digitonin-permeabilized cells: role of the P58 containing compartment
    H Plutner
    Department of Cell and Molecular Biology, Scripps Research Institute, La Jolla, California 92037
    J Cell Biol 119:1097-116. 1992
    ....
  38. ncbi Cloning, expression and characterization of endo-beta-1,4-mannanase from Aspergillus fumigatus in Aspergillus sojae and Pichia pastoris
    Gökhan Duruksu
    Dept of Biotechnology, Middle East Technical University, Ankara, Turkey
    Biotechnol Prog 25:271-6. 2009
    ..5 and 5.2 for A. sojae and P. pastoris, respectively. The enzyme from P. pastoris was more stable retaining most of the activity up to 50 degrees C, whereas the enzyme from A. sojae rapidly lost activity above 40 degrees C...
  39. ncbi Cyclic amidine sugars as transition-state analogue inhibitors of glycosidases: potent competitive inhibitors of mannosidases
    Marie Pierre Heck
    CEA, CE Saclay, Service de Marquage Moleculaire et de Chimie Bioorganique, Bat 547, Departement de Biologie Joliot Curie, 91191 Gif sur Yvette, France
    J Am Chem Soc 126:1971-9. 2004
    ..9b bearing an exocyclic ethylamine moiety proved to be a selective competitive inhibitor of alpha- and beta-mannosidases (K(i) = 6 nM) making it the most potent inhibitor of these glycosidases reported to date...
  40. pmc Processing of N-linked glycans during endoplasmic-reticulum-associated degradation of a short-lived variant of ribophorin I
    Claudia Kitzmuller
    Max F Perutz Laboratories, University Departments at the Vienna Biocenter and Institute of Medical Biochemistry, Department of Molecular Genetics, University of Vienna, Austria
    Biochem J 376:687-96. 2003
    ..N-glycan species, in particular Glc(1)Man(7)GlcNAc(2), were also found on the protein, indicating that several mannosidases might be implicated in the initial trimming of the oligomannoside...
  41. ncbi Comparison of kifunensine and 1-deoxymannojirimycin binding to class I and II alpha-mannosidases demonstrates different saccharide distortions in inverting and retaining catalytic mechanisms
    Niket Shah
    Department of Medical Biophysics, University of Toronto, Ontario, Canada
    Biochemistry 42:13812-6. 2003
    b>Mannosidases are key enzymes in the eukaryotic N-glycosylation pathway. These enzymes fall into two broad classes (I and II) and are characteristically different in catalytic mechanism, sequence, and structure...
  42. doi Optimization of the production of thermostable endo-beta-1,4 mannanases from a newly isolated Aspergillus niger gr and Aspergillus flavus gr
    Naganagouda V Kote
    Department of Biochemistry, Gulbarga University, Karnataka, India
    Appl Biochem Biotechnol 152:213-23. 2009
    ..These new strains offer an attractive alternative source of enzymes for the food and feed processing industries...
  43. ncbi Molecular cloning and characterization of Arabidopsis thaliana Golgi alpha-mannosidase II, a key enzyme in the formation of complex N-glycans in plants
    Richard Strasser
    Institute of Applied Genetics and Cell Biology, Vienna, Austria
    Plant J 45:789-803. 2006
    ..A GFP fusion construct containing only the transmembrane domain was predominantly retained in the ER, a result that indicates the presence of a motif promoting ER export within the last 10 amino acids of the cytoplasmic tail of AtGMII...
  44. ncbi A highly thermostable endo-(1,4)-beta-mannanase from the marine bacterium Rhodothermus marinus
    O Politz
    Department of Dermatology, Benjamin Franklin Medical Centre, The Free University of Berlin, Germany
    Appl Microbiol Biotechnol 53:715-21. 2000
    ..In contrast, R. marinus-derived protein retained 87% activity after 1 h at 90 degrees C. The enzyme hydrolysed carob-galactomannan (locust bean gum) effectively and to a smaller extent guar gum, but not yeast mannan...
  45. ncbi Characterization of a cDNA encoding a novel human Golgi alpha 1, 2-mannosidase (IC) involved in N-glycan biosynthesis
    L O Tremblay
    McGill Cancer Centre, McGill University, Montreal, Quebec H3G 1Y6, Canada
    J Biol Chem 275:31655-60. 2000
    A human cDNA encoding a 70.9-kDa type II membrane protein with sequence similarity to class I alpha1,2-mannosidases was isolated...
  46. ncbi A cellulose-binding module of the Trichoderma reesei beta-mannanase Man5A increases the mannan-hydrolysis of complex substrates
    Per Hägglund
    Department of Biochemistry, Centre for Chemistry and Chemical Engineering, Lund University, PO Box 124, S 221 00 Lund, Sweden
    J Biotechnol 101:37-48. 2003
    ..Moreover, the binding of Man5A to cellulose was compared with that of an endoglucanase (Cel7B) from T. reesei...
  47. pmc A temperature-sensitive dcw1 mutant of Saccharomyces cerevisiae is cell cycle arrested with small buds which have aberrant cell walls
    Hiroshi Kitagaki
    National Research Institute of Brewing, 3 7 1, Kagamiyama, Higashihiroshima, Hiroshima, Japan 739 0046
    Eukaryot Cell 3:1297-306. 2004
    ..DCW1 and DFG5 mRNA levels peaked in the G1 and S phases, respectively. These results indicate that Dcw1p and Dfg5p are involved in bud formation through their involvement in biogenesis of the bud cell wall...
  48. ncbi N-Glycosylation affects endoplasmic reticulum degradation of a mutated derivative of carboxypeptidase yscY in yeast
    M Knop
    Institut fuer Biochemie, Universitaet Stuttgart, Germany
    Yeast 12:1229-38. 1996
    ..Our results suggest that the ER of yeast contains a glycosylation-dependent quality control system, as has been shown for higher eukaryotic cells...
  49. pmc A germination-specific endo-beta-mannanase gene is expressed in the micropylar endosperm cap of tomato seeds
    H Nonogaki
    Department of Vegetable Crops, University of California, Davis, California 95616 8631, USA
    Plant Physiol 123:1235-46. 2000
    ..Distinct mannanases are involved in germination and post-germinative growth, with LeMAN2 being associated with endosperm cap weakening prior to radicle emergence, whereas LeMAN1 mobilizes galactomannan reserves in the lateral endosperm...
  50. ncbi Modification of the N-glycosylation pathway to produce homogeneous, human-like glycans using GlycoSwitch plasmids
    Wouter Vervecken
    Department of Molecular Biomedical Research, Ghent University, Ghent, Belgium
    Methods Mol Biol 389:119-38. 2007
    ..Different plasmids are described as well as glycan analysis methods...
  51. ncbi Two homologous genes, DCW1 (YKL046c) and DFG5, are essential for cell growth and encode glycosylphosphatidylinositol (GPI)-anchored membrane proteins required for cell wall biogenesis in Saccharomyces cerevisiae
    Hiroshi Kitagaki
    National Research Institute of Brewing, Higashihiroshima, Japan
    Mol Microbiol 46:1011-22. 2002
    ..These results suggest that both Dcw1p and Dfg5p are GPI-anchored membrane proteins and are required for normal biosynthesis of the cell wall...
  52. ncbi Microbial mannanases: an overview of production and applications
    Samriti Dhawan
    Department of Biotechnology, GGDSD College, Chandigarh, India
    Crit Rev Biotechnol 27:197-216. 2007
    ..The reference to plant and animal mannanases has been made to complete the overview. However, the major emphasis of the review is on the microbial mannanases...
  53. ncbi Molecular and biochemical characterization of endo-beta-mannanases from germinating coffee (Coffea arabica) grains
    P Marraccini
    Department of Plant Science, Nestle Research Centre, Tours, France
    Planta 213:296-308. 2001
    ..Activity and mRNA levels appeared to be tightly co-ordinated. Tests of substrate specificity with the purified ManB enzyme showed that activity required a minimum of five mannose units to function efficiently...
  54. ncbi High-resolution native and complex structures of thermostable beta-mannanase from Thermomonospora fusca - substrate specificity in glycosyl hydrolase family 5
    M Hilge
    Laboratorium für Biochemie ETH Zentrum Universitätstrasse 16 CH 8092 Zürich, Switzerland
    Structure 6:1433-44. 1998
    ..No three-dimensional structure of a mannan-degrading enzyme has been reported until now...
  55. ncbi The alpha-mannosidases: phylogeny and adaptive diversification
    D S Gonzalez
    Department of Medical Microbiology, University of Georgia, USA
    Mol Biol Evol 17:292-300. 2000
    ..These data point to a possible role for positive Darwinian selection in the evolution of alpha-mannosidase functional diversification following gene duplication...
  56. ncbi Structural basis for catalysis and inhibition of N-glycan processing class I alpha 1,2-mannosidases
    F Vallee
    Program in Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 275:41287-98. 2000
    ..The structures also provide insight into the specificity of this class of enzymes and provide a blueprint for the future design of novel inhibitors that prevent degradation of misfolded proteins in genetic diseases...
  57. pmc Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans
    K W Moremen
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139
    J Cell Biol 115:1521-34. 1991
    ..Partial human alpha-mannosidase II cDNA clones were also isolated and the gene was localized to human chromosome 5...
  58. ncbi Insights into the mechanism of Drosophila melanogaster Golgi alpha-mannosidase II through the structural analysis of covalent reaction intermediates
    Shin Numao
    Department of Chemistry, University of British Columbia, Vancouver, British Columbia V6T 1Z1, Canada
    J Biol Chem 278:48074-83. 2003
    ..J., Szabo, L., Stoll, D., Withers, S. G., and Davies, G. J. (2002) Angew. Chem. Int. Ed. Engl. 41, 2824-2827) suggests that these disparate enzymes have recruited common stereoelectronic features in evolving their catalytic mechanisms...
  59. ncbi Immunocytochemical localization of the Menkes copper transport protein (ATP7A) to the trans-Golgi network
    H A Dierick
    Department of Pediatrics, University of Michigan, Ann Arbor 48109 0618, USA
    Hum Mol Genet 6:409-16. 1997
    ....
  60. ncbi Production of complex human glycoproteins in yeast
    Stephen R Hamilton
    Thayer School of Engineering and the Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA
    Science 301:1244-6. 2003
    ..endogenous yeast glycosylation pathways, while properly localizing five active eukaryotic proteins, including mannosidases I and II, N-acetylglucosaminyl transferases I and II, and uridine 5'-diphosphate (UDP)-N-acetylglucosamine ..
  61. ncbi Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of alpha1,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25
    Satoshi Inoue
    Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
    Eur J Biochem 271:356-66. 2004
    ..results suggest that fhx/P25 in the elementary unit is largely protected from digestion with Golgi alpha1,2-mannosidases when L-chains are present in the unit...
  62. ncbi A novel Rab GTPase, Rab33B, is ubiquitously expressed and localized to the medial Golgi cisternae
    J Y Zheng
    Section of Bacterial Infection, Institute of Immunological Science, Hokkaido University, Sapporo, Japan
    J Cell Sci 111:1061-9. 1998
    ..Immunoelectron microscopy analysis further defined the localization of Rab33B to the medial Golgi cisternae. These results suggest Rab33B plays a role in intra-Golgi transport...
  63. ncbi The involvement of mnn4 and mnn6 mutations in mannosylphosphorylation of O-linked oligosaccharide in yeast Saccharomyces cerevisiae
    K Nakayama
    Molecular Biology Department, National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, Japan
    Biochim Biophys Acta 1425:255-62. 1998
    ..The amount of mannosylphosphorylated mannotriose was 7% of total O-linked oligosaccharides (20% of neutral mannotriose) of chitinase in strain mnn1...
  64. ncbi A combined STD-NMR/molecular modeling protocol for predicting the binding modes of the glycosidase inhibitors kifunensine and salacinol to Golgi alpha-mannosidase II
    Xin Wen
    Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6
    Biochemistry 44:6729-37. 2005
    ....
  65. ncbi Synthesis of an octamannosyled glycan chain, the key oligosaccharide structure in ER-associated degradation
    Ichiro Matsuo
    RIKEN, The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    Carbohydr Res 338:2163-8. 2003
    ..The oligosaccharide assembly was performed in a convergent and stereoselective manner, using three oligosaccharide components, a core trisaccharide having a beta-mannoside bond, a liner mannotriose, and a branched mannotetraose...
  66. ncbi Alkaloidal components in the poisonous plant, Ipomoea carnea (Convolvulaceae)
    Mitsue Haraguchi
    Animal Health Center, Biological Institute of Sao Paulo, Sao Paulo, Brazil
    J Agric Food Chem 51:4995-5000. 2003
    ..1, 0.75, and 0.84 microM, respectively, and alkaloid 5 was a moderate inhibitor of alpha- and beta-mannosidases. Although alkaloid 1 is known as a powerful inhibitor of lysosomal alpha-mannosidase (IC(50) = 0...
  67. ncbi Isolation of a mouse Golgi mannosidase cDNA, a member of a gene family conserved from yeast to mammals
    A Herscovics
    McGill Cancer Centre, McGill University, Montreal, Quebec, Canada
    J Biol Chem 269:9864-71. 1994
    ..the deduced amino acid sequence of a rabbit liver alpha-mannosidase partial cDNA, demonstrating that processing mannosidases have been conserved through eukaryotic evolution...
  68. ncbi Man9-mannosidase from human kidney is expressed in COS cells as a Golgi-resident type II transmembrane N-glycoprotein
    E Bieberich
    Institut fur Physiologische Chemie, Bonn, Germany
    Eur J Biochem 233:644-9. 1995
    ..This localization clearly differs from that of pig liver Man9-mannosidase which appears to be located in the endoplasmic reticulum and transient vesicles...
  69. ncbi The synthesis of a series of modified mannotrisaccharides as probes of the enzymes involved in the early stages of mammalian complex N-glycan formation
    Chris A Tarling
    Department of Chemistry, 2036 Main Mall, University of British Columbia, Vancouver, BC, Canada V6T 1Z1
    Carbohydr Res 339:2487-97. 2004
    ..Secondly it should render trisaccharide 8 stable to hydrolysis by the mannosidases Man-II and Man-III by inductive destabilization of their oxocarbenium ion-like transition states...
  70. ncbi The roles of enzyme localisation and complex formation in glycan assembly within the Golgi apparatus
    Christopher L de Graffenried
    Department of Chemistry, University of California, Berkeley, California 94720 1460, USA
    Curr Opin Cell Biol 16:356-63. 2004
    ..Together, localisation and association govern the assembly of complex glycans and thereby regulate interactions at the cell surface...
  71. ncbi A novel stress-induced EDEM variant regulating endoplasmic reticulum-associated glycoprotein degradation
    Silvia Olivari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    J Biol Chem 280:2424-8. 2005
    ....
  72. ncbi Transient calnexin interaction confers long-term stability on folded K+ channel protein in the ER
    Rajesh Khanna
    Department of Physiology and Molecular Biology Institute, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095 1751, USA
    J Cell Sci 117:2897-908. 2004
    ..Our results indicate that transient interaction with calnexin provides long-term protection from ER-associated degradation...
  73. ncbi Isolation, characterization, and expression of cDNA encoding a rat liver endoplasmic reticulum alpha-mannosidase
    J Bischoff
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    J Biol Chem 265:17110-7. 1990
    ..The rat liver endoplasmic reticulum alpha-mannosidase bears striking homology to the vacuolar alpha-mannosidase from Saccharomyces cerevisiae...
  74. ncbi SS33410, an inhibitor of V-ATPase, blocks intracellular protein transport of the VSV-G protein in the Golgi compartment
    Dae Hyun Seog
    Department of Microbiology, College of Medicine, Inje University, Busan, Korea
    Biosci Biotechnol Biochem 67:2591-7. 2003
    ..These studies thus suggest that SS33410 blocks the intracellular protein transport before the step of trimming by mannosidase II, which is confined to the medial Golgi compartment...
  75. ncbi The soluble form of rat liver alpha-mannosidase is immunologically related to the endoplasmic reticulum membrane alpha-mannosidase
    J Bischoff
    J Biol Chem 261:4758-65. 1986
    ..In contrast, as shown in the accompanying paper, the intracellular action of ER alpha-mannosidase on glycoprotein-bound Man9GlcNAc2 is highly specific...
  76. ncbi Effects of mitogenic stimulation on lymphocyte alpha-D-mannosidases
    D Abraham
    Eur J Cell Biol 40:167-75. 1986
    ..The intracellular localization of the alpha-D-mannosidases in the non-stimulated and activated murine cells was investigated by fractionation of lymphocyte lysates on ..
  77. ncbi Synthesis of FimH receptor-active manno-oligosaccharides by reverse hydrolysis using alpha-mannosidases from Penicillium citrinum, Aspergillus phoenicis and almond
    V Maitin
    School of Food Biosciences, The University of Reading, Whiteknights, PO Box 226, RG6 6AP, Reading, UK
    Appl Microbiol Biotechnol 63:666-71. 2004
    ..4% purity. The synthesised oligosaccharides can potentially inhibit the adhesion of pathogens by acting as "decoys" of receptors of type-1 fimbriae carried by enterobacteria...
  78. pmc Steady-state localization of a medial-Golgi glycosyltransferase involves transit through the trans-Golgi network
    A S Opat
    Department of Pathology and Immunology, Monash University Medical School, Commercial Road, Prahran, Melbourne, Victoria 3181, Australia
    Biochem J 358:33-40. 2001
    ..Furthermore, this data suggests that retrograde transport pathways play an important role in establishing the asymmetric distribution of GlcNAc-TI within the Golgi stack...
  79. ncbi Structure of Penicillium citrinum alpha 1,2-mannosidase reveals the basis for differences in specificity of the endoplasmic reticulum and Golgi class I enzymes
    Yuri D Lobsanov
    Program in Structural Biology and Biochemistry, Research Institute, The Hospital for Sick Children, 555 University Ave, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 277:5620-30. 2002
    Class I alpha1,2-mannosidases (glycosylhydrolase family 47) are key enzymes in the maturation of N-glycans. This protein family includes two distinct enzymatically active subgroups...
  80. ncbi Synthesis, enzymatic activity, and X-ray crystallography of an unusual class of amino acids
    Wang Chen
    Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada V5A 1S6
    Bioorg Med Chem 14:8332-40. 2006
    ..The same compound inhibits recombinant human maltase glucoamylase, one of the key intestinal enzymes involved in the breakdown of glucose oligosaccharides in the small intestine, with a K(i) value of 21microM...
  81. ncbi Overexpression of the Golgi-localized enzyme alpha-mannosidase IIx in Chinese hamster ovary cells results in the conversion of hexamannosyl-N-acetylchitobiose to tetramannosyl-N-acetylchitobiose in the N-glycan-processing pathway
    M Oh-Eda
    Glycobiology Program, The Burnham Institute, La Jolla, CA 92037, USA
    Eur J Biochem 268:1280-8. 2001
    ....
  82. ncbi The importance of trimming reactions on asparagine-linked oligosaccharides for protein quality control
    Jürgen Roth
    Division of Cell and Molecular Pathology, Department of Pathology, University of Zurich, Schmelzbergstrasse 12, 8091 Zurich, Switzerland
    Histochem Cell Biol 117:159-69. 2002
    Although the biochemistry of early trimming reactions by glucosidases and ER mannosidases occurring on asparagine-linked oligosaccharides has been known for a long time, their involvement in quality control of protein folding has become ..
  83. ncbi Enzymatic glycosylation of lincomycin
    L Weignerova
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague
    Biosci Biotechnol Biochem 65:1897-9. 2001
    ..Compound 1 was glycosylated using jack bean alpha-mannosidase to produce 7-O-alpha-D-mannopyranosyl-lincomycin (2)...
  84. ncbi 5-Methylthiopentose: a new substituent on lipoarabinomannan in Mycobacterium tuberculosis
    Achim Treumann
    Astbury Centre for Structural Molecular Biology, School of Biochemistry and Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK
    J Mol Biol 316:89-100. 2002
    ..We suggest interference with the signal transduction mechanisms of infected macrophages as a possible function for this newly discovered LAM substituent...
  85. pmc Reevaluation of the effects of brefeldin A on plant cells using tobacco Bright Yellow 2 cells expressing Golgi-targeted green fluorescent protein and COPI antisera
    Christophe Ritzenthaler
    Institut de Biologie Moleculaire des Plantes, 67084 Strasbourg Cedex, France
    Plant Cell 14:237-61. 2002
    ....
  86. pmc Endoplasmic reticulum (ER) mannosidase I is compartmentalized and required for N-glycan trimming to Man5-6GlcNAc2 in glycoprotein ER-associated degradation
    Edward Avezov
    Department of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Mol Biol Cell 19:216-25. 2008
    ..When newly made glycoproteins cannot fold properly, transport through the ERQC leads to trimming of a critical number of mannose residues, triggering a signal for degradation...
  87. ncbi Intestinal dipeptidyl peptidase IV is efficiently sorted to the apical membrane through the concerted action of N- and O-glycans as well as association with lipid microdomains
    Marwan Alfalah
    Department of Physiological Chemistry, School of Veterinary Medicine Hannover, Hannover D 30559, Germany
    J Biol Chem 277:10683-90. 2002
    ....
  88. ncbi Matrix proteins can generate the higher order architecture of the Golgi apparatus
    J Seemann
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520 8002, USA
    Nature 407:1022-6. 2000
    ..This new concept of the Golgi further indicates that the Golgi may be an autonomous organelle rather than one that is in simple dynamic equilibrium with the endoplasmic reticulum...
  89. pmc Engineering the protein N-glycosylation pathway in insect cells for production of biantennary, complex N-glycans
    Jason Hollister
    Department of Molecular Biology, University of Wyoming, Laramie 82071, USA
    Biochemistry 41:15093-104. 2002
    ....
  90. ncbi Role of N-glycan-dependent quality control in the cell-surface expression of the AT1 receptor
    Pascal M Lanctot
    Department of Pharmacology, Faculty of Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, Que, Canada J1H 5N4
    Biochem Biophys Res Commun 340:395-402. 2006
    ....
  91. ncbi Alterations in the post-translational modification and intracellular trafficking of clusterin in MCF-7 cells during apoptosis
    J O'Sullivan
    Department of Biological Sciences, University of Notre Dame, Notre Dame IN 46556, USA
    Cell Death Differ 10:914-27. 2003
    ..These data suggest that, at least in secretory epithelial cells, retrograde transport from the Golgi to the ER of a nonglycosylated, uncleaved isoform and the subsequent translocation of clusterin to the nucleus occur in dying cells...
  92. ncbi Cloning, expression, purification, and characterization of the acid alpha-mannosidase from Trypanosoma cruzi
    A S Vandersall-Nairn
    Complex Carbohydrate Research Center and the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
    Glycobiology 8:1183-94. 1998
    ..A polyclonal antibody raised to the recombinant enzyme was shown to immunoprecipitate the alpha-mannosidase from T.cruzi cell extracts and will be used in future immunolocalization studies...
  93. ncbi The role of glucosidase II and endomannosidase in glucose trimming of asparagine-linked oligosaccharides
    Jürgen Roth
    Division of Cell and Molecular Pathology, Department of Pathology, University of Zurich, Switzerland
    Biochimie 85:287-94. 2003
    ..The mutually exclusive subcellular distribution of glucosidase II and endomannosidase are discussed in terms of their significance for quality control of protein folding and N-glycosylation...
  94. ncbi Identification, expression, and characterization of a cDNA encoding human endoplasmic reticulum mannosidase I, the enzyme that catalyzes the first mannose trimming step in mammalian Asn-linked oligosaccharide biosynthesis
    D S Gonzalez
    Complex Carbohydrate Research Center and the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
    J Biol Chem 274:21375-86. 1999
    ..sequence tag clones were identified by sequence similarity to mammalian and yeast oligosaccharide-processing mannosidases, and the full-length coding region of the putative mannosidase homolog was isolated by a combination of 5'-..
  95. ncbi Endomannosidase processes oligosaccharides of alpha1-antitrypsin and its naturally occurring genetic variants in the Golgi apparatus
    T Torossi
    Department of Pathology, Division of Cell and Molecular Pathology, University of Zurich, Schmelzbergstrasse 12, 8091, Zurich, Switzerland
    Cell Mol Life Sci 63:1923-32. 2006
    ..For exported misfolded glycoproteins, this would provide a pathway for the formation of mature oligosaccharides important for their proper trafficking and correct functioning...
  96. ncbi The structure of bovine lysosomal alpha-mannosidase suggests a novel mechanism for low-pH activation
    Pirkko Heikinheimo
    Institutt for Kjemi, Universitetet i Tromsø, N 9037, Tromsø, Norway
    J Mol Biol 327:631-44. 2003
    ..Furthermore, it provides a basis for understanding the human form of alpha-mannosidosis at the atomic level. The atomic coordinates and structure factors have been deposited in the Protein Data Bank (accession codes 1o7d and r1o7dsf)...
  97. ncbi N-linked oligosaccharide processing, but not association with calnexin/calreticulin is highly correlated with endoplasmic reticulum-associated degradation of antithrombin Glu313-deleted mutant
    Fuminori Tokunaga
    Department of Life Science, Graduate School of Science, Himeji Institute of Technology, Harima Science Garden City, Hyogo 678 1297, Japan
    Arch Biochem Biophys 411:235-42. 2003
    ....
  98. ncbi Expression and role of mannose receptor/terminal high-mannose type oligosaccharide on osteoclast precursors during osteoclast formation
    S Morishima
    Cellular Physiological Chemistry, Tokyo Medical and Dental University, 1 5 45, Yushima, Bunkyo ku, Tokyo 113 8549, Japan
    J Endocrinol 176:285-92. 2003
    ..These results indicate that the binding of terminal high-mannose and mannose receptor is important for the process of cellular fusion in osteoclast formation...
  99. ncbi Polysaccharide analysis using carbohydrate gel electrophoresis: a method to study plant cell wall polysaccharides and polysaccharide hydrolases
    Florence Goubet
    Department of Biochemistry, Cambridge University, Building O, Downing Site, Cambridge, CB2 1QW, United Kingdom
    Anal Biochem 300:53-68. 2002
    ..PACE can detect as little as 500 fmol of monosaccharides and 100 fmol of oligosaccharides, and it is fast and quantitative...
  100. pmc Structure and kinetic investigation of Streptococcus pyogenes family GH38 alpha-mannosidase
    Michael D L Suits
    York Structural Biology Laboratory, Department of Chemistry, University of York, York, United Kingdom
    PLoS ONE 5:e9006. 2010
    The enzymatic hydrolysis of alpha-mannosides is catalyzed by glycoside hydrolases (GH), termed alpha-mannosidases. These enzymes are found in different GH sequence-based families...
  101. doi The molecular characterization of a novel GH38 α-mannosidase from the crenarchaeon Sulfolobus solfataricus revealed its ability in de-mannosylating glycoproteins
    Beatrice Cobucci-Ponzano
    Institute of Protein Biochemistry Consiglio Nazionale delle Ricerche, Via P Castellino 111, 80131 Naples, Italy
    Biochimie 92:1895-907. 2010
    b>Mannosidases, important enzymes in the N-glycan processing and degradation in Eukaryotes, are frequently found in the genome of Bacteria and Archaea in which their function is still largely unknown...

Research Grants67

  1. ALPHA-MANNOSIDASES IN MAMMALIAN GLYCOPROTEIN PROCESSING
    Kelley Moremen; Fiscal Year: 1993
    ..the regulation, structure, and function of resident enzymes of the Golgi complex by examining the alpha-mannosidases of the N-glycan processing pathway...
  2. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2000
    ..The first family consists of Class I al,2-mannosidases that have been conserved through eukaryotic evolution from yeast to man...
  3. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 1999
    ..The first family consists of Class I al,2-mannosidases that have been conserved through eukaryotic evolution from yeast to man...
  4. CARHOHYDRATE MOIETIES OF GP120
    Khushi Matta; Fiscal Year: 1993
    ..studies toward the chemical synthesis of various potential inhibitors of glycosidases, such as glucosidases and mannosidases, involved in the processing and biosynthesis of glycoprotein including gp120...
  5. CARHOHYDRATE MOIETIES OF GP120
    Khushi Matta; Fiscal Year: 1991
    ..studies toward the chemical synthesis of various potential inhibitors of glycosidases, such as glucosidases and mannosidases, involved in the processing and biosynthesis of glycoprotein including gp120...
  6. ALPHA-MANNOSIDASES IN MAMMALIAN GLYCOPROTEIN PROCESSING
    Kelley Moremen; Fiscal Year: 1992
    ..the regulation, structure, and function of resident enzymes of the Golgi complex by examining the alpha-mannosidases of the N-glycan processing pathway...
  7. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2003
    ..The first enzymes are Class I al,2-mannosidases that have been conserved in all eukaryotes...
  8. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2001
    ..The first enzymes are Class I al,2-mannosidases that have been conserved in all eukaryotes...
  9. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2005
    ..The first enzymes are Class I al,2-mannosidases that have been conserved in all eukaryotes...
  10. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2004
    ..The first enzymes are Class I al,2-mannosidases that have been conserved in all eukaryotes...
  11. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2002
    ..The first enzymes are Class I al,2-mannosidases that have been conserved in all eukaryotes...
  12. Mannosidase inhibitors as therapeutics for glycoprotein misfolding diseases
    Kelley Moremen; Fiscal Year: 2009
    ..They also inhibit glycan processing alpha-mannosidases in the Golgi complex and block maturation to complex type glycan structures on cell surface and secreted ..
  13. Mannosidase inhibitors as therapeutics for glycoprotein misfolding diseases
    Kelley Moremen; Fiscal Year: 2007
    ..They also inhibit glycan processing alpha-mannosidases in the Golgi complex and block maturation to complex type glycan structures on cell surface and secreted ..
  14. Mannosidase inhibitors as therapeutics for glycoprotein misfolding diseases
    Kelley Moremen; Fiscal Year: 2006
    ..They also inhibit glycan processing alpha-mannosidases in the Golgi complex and block maturation to complex type glycan structures on cell surface and secreted ..
  15. Mannosidase inhibitors as therapeutics for glycoprotein misfolding diseases
    Kelley W Moremen; Fiscal Year: 2010
    ..They also inhibit glycan processing alpha-mannosidases in the Golgi complex and block maturation to complex type glycan structures on cell surface and secreted ..
  16. PATHOBIOGENESIS OF HERITABLE PULMONARY EMPHYSEMA
    Richard Sifers; Fiscal Year: 2002
    ..The hypothesis to be tested is that modification by intracellular mannosidases sorts genetic variant PIZ for non-proteasomal elimination by promoting specific sequential interactions with ..
  17. PATHOBIOGENESIS OF HERITABLE PULMONARY EMPHYSEMA
    Richard Sifers; Fiscal Year: 2001
    ..The hypothesis to be tested is that modification by intracellular mannosidases sorts genetic variant PIZ for non-proteasomal elimination by promoting specific sequential interactions with ..
  18. PATHOBIOGENESIS OF HERITABLE PULMONARY EMPHYSEMA
    Richard Sifers; Fiscal Year: 2000
    ..The hypothesis to be tested is that modification by intracellular mannosidases sorts genetic variant PIZ for non-proteasomal elimination by promoting specific sequential interactions with ..
  19. TRANSIENT GLUCOSYLATION OF GLYCOPROTEINS
    Armando Parodi; Fiscal Year: 1991
    ..shows a different specificity with respect to the oligosaccharides, this will indicate that processing by ER mannosidases influences the glycosylation of glycoproteins...
  20. TRANSIENT GLUCOSYLATION OF GLYCOPROTEINS
    Armando Parodi; Fiscal Year: 1990
    ..shows a different specificity with respect to the oligosaccharides, this will indicate that processing by ER mannosidases influences the glycosylation of glycoproteins...
  21. TRANSIENT GLUCOSYLATION OF GLYCOPROTEINS
    Armando Parodi; Fiscal Year: 1992
    ..shows a different specificity with respect to the oligosaccharides, this will indicate that processing by ER mannosidases influences the glycosylation of glycoproteins...
  22. NOVEL GLYCOSIDASE INHIBITORS AS ANTICANCER AGENTS
    BRIAN COPPOLA; Fiscal Year: 2002
    ..Second, SW causes an undesired inhibition of lysosomal alpha- mannosidases, which induces a reversible phenocopy of the genetic lysosomal storage disease alpha-mannosidosis...
  23. NOVEL GLYCOSIDASE INHIBITORS AS ANTICANCER AGENTS
    William Pearson; Fiscal Year: 2000
    ..Second, SW causes an undesired inhibition of lysosomal alpha- mannosidases, which induces a reversible phenocopy of the genetic lysosomal storage disease alpha-mannosidosis...
  24. NOVEL GLYCOSIDASE INHIBITORS AS ANTICANCER AGENTS
    William Pearson; Fiscal Year: 2001
    ..Second, SW causes an undesired inhibition of lysosomal alpha- mannosidases, which induces a reversible phenocopy of the genetic lysosomal storage disease alpha-mannosidosis...
  25. NOVEL GLYCOSIDASE INHIBITORS AS ANTICANCER AGENTS
    William Pearson; Fiscal Year: 1999
    ..Second, SW causes an undesired inhibition of lysosomal alpha- mannosidases, which induces a reversible phenocopy of the genetic lysosomal storage disease alpha-mannosidosis...
  26. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2005
    ..The first family consists of Class I alpha1,2-mannosidases that have been conserved in all eukaryotes...
  27. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2007
    ..The first family consists of Class I alpha1,2-mannosidases that have been conserved in all eukaryotes...
  28. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 2006
    ..The first family consists of Class I alpha1,2-mannosidases that have been conserved in all eukaryotes...
  29. Mannosidase inhibitors as therapeutics for glycoprotein misfolding diseases
    Kelley Moremen; Fiscal Year: 2009
    ..They also inhibit glycan processing a-mannosidases in the Golgi complex and block maturation to complex type glycan structures on cell surface and secreted ..
  30. CELLULAR PROTEIN MATURATION AND DEGRADATION
    Daniel Hebert; Fiscal Year: 2009
    ..Therefore, mannosidases appear to be involved in generating the quality control code that is recognized by carbohydrate-binding sorting ..
  31. CELLULAR PROTEIN MATURATION AND DEGRADATION
    Daniel N Hebert; Fiscal Year: 2010
    ..Therefore, mannosidases appear to be involved in generating the quality control code that is recognized by carbohydrate-binding sorting ..
  32. STRUCTURE, BIOSYNTHESIS AND FUNCTION OF GLYCOPROTEINS
    Stuart Kornfeld; Fiscal Year: 1980
    ..We plan to isolate and characterize the glucosidases and mannosidases which carry out processing...
  33. MANNOPROTEIN BIOSYNTHESIS
    Annette Herscovics; Fiscal Year: 1992
    ..We are particularly interested in processing reactions which involve alpha-mannosidases and mannosyltransferases...
  34. MANNOPROTEIN BIOSYNTHESIS
    Annette Herscovics; Fiscal Year: 1993
    ..We are particularly interested in processing reactions which involve alpha-mannosidases and mannosyltransferases...
  35. Selective Mannosidase Inhibitors as Cancer Therapeutics
    Kelley Moremen; Fiscal Year: 2003
    ..Significant differences in the substrate binding specificities of these two mannosidases can now be exploited to develop an inhibitor that is selective for Golgi alpha-mannosidase II...
  36. Selective Mannosidase Inhibitors as Cancer Therapeutics
    Kelley Moremen; Fiscal Year: 2004
    ..Significant differences in the substrate binding specificities of these two mannosidases can now be exploited to develop an inhibitor that is selective for Golgi alpha-mannosidase II...
  37. Selective Mannosidase Inhibitors as Cancer Therapeutics
    Kelley Moremen; Fiscal Year: 2001
    ..Significant differences in the substrate binding specificities of these two mannosidases can now be exploited to develop an inhibitor that is selective for Golgi alpha-mannosidase II...
  38. Selective Mannosidase Inhibitors as Cancer Therapeutics
    Kelley Moremen; Fiscal Year: 2002
    ..Significant differences in the substrate binding specificities of these two mannosidases can now be exploited to develop an inhibitor that is selective for Golgi alpha-mannosidase II...
  39. Mannosidases in Glycoprotein Biosynthesis & Catabolism
    Kelley Moremen; Fiscal Year: 2005
    ..We have focused on the Class I (family 47) a-mannosidases in the N-glycan pathway since they act as committed steps in the synthesis of complex oligosaccharides by ..
  40. Mannosidases in Glycoprotein Biosynthesis & Catabolism
    Kelley Moremen; Fiscal Year: 2006
    ..We have focused on the Class I (family 47) a-mannosidases in the N-glycan pathway since they act as committed steps in the synthesis of complex oligosaccharides by ..
  41. MANNOSE IN MAMMALIAN GLYCOPROTEIN SYNTHESIS
    Hudson Freeze; Fiscal Year: 2001
    ..4. Demonstrate glycoprotein digestion and alpha-mannosidases responsible for it...
  42. Mannosidases in Glycoprotein Biosynthesis & Catabolism
    Kelley Moremen; Fiscal Year: 2007
    ..We have focused on the Class I (family 47) a-mannosidases in the N-glycan pathway since they act as committed steps in the synthesis of complex oligosaccharides by ..
  43. GLYCOPROTEIN PROCESSING SYSTEMS OF ER AND GOLGI
    PHILLIPS ROBBINS; Fiscal Year: 1991
    ..The cloning and sequencing of mammalian Golgi processing mannosidases represents another major emphasis...
  44. MANNOSE IN MAMMALIAN GLYCOPROTEIN SYNTHESIS
    Hudson Freeze; Fiscal Year: 1999
    ..4. Demonstrate glycoprotein digestion and alpha-mannosidases responsible for it...
  45. MANNOSE IN MAMMALIAN GLYCOPROTEIN SYNTHESIS
    Hudson Freeze; Fiscal Year: 2000
    ..4. Demonstrate glycoprotein digestion and alpha-mannosidases responsible for it...
  46. MANNOSIDASES IN GLYCOPROTEIN BIOSYNTHESIS AND CATABOLISM
    Kelley Moremen; Fiscal Year: 2000
    ..To address these problems the cDNAs encoding several of the processing and catabolic alpha-mannosidases have been isolated and have been organized into two multigene classes...
  47. MANNOSIDASES IN GLYCOPROTEIN BIOSYNTHESIS AND CATABOLISM
    Kelley Moremen; Fiscal Year: 1999
    ..To address these problems the cDNAs encoding several of the processing and catabolic alpha-mannosidases have been isolated and have been organized into two multigene classes...
  48. BIOCHEMICAL STUDIES ON LYSOSOMES AND RELATED ENZYMES
    OSCAR TOUSTER; Fiscal Year: 1980
    ..The several alpha-D-mannosidases and alpha-D-glucosidases will be studied with oligosaccharide substrates, particularly the biosynthetic ..
  49. Induction of 2G12 neutralizing antibody by plant-derived HIVgp145
    Yvonne Rosenberg; Fiscal Year: 2007
    ..of HIV gp145 to the endoplasmic reticulum using a KDEL tag which blocks exit into the Golgi and exposure to mannosidases. These high-mannose HIV Env molecules exhibited stronger reactivity with 2G12 than CHO-derived Env, suggesting ..
  50. ROLE OF CALSEQUESTRIN AND ITS PHOSPHORYLATION IN HEART
    Steven Cala; Fiscal Year: 2005
    ..Comparing frozen tissue samples of control and heart failure, we will determine whether triadin-1 glycans are a so defective, and whether differences exist in ER/Golgi mannosidases.
  51. ROLE OF CALSEQUESTRIN AND ITS PHOSPHORYLATION IN HEART
    Steven Cala; Fiscal Year: 2007
    ..Comparing frozen tissue samples of control and heart failure, we will determine whether triadin-1 glycans are a so defective, and whether differences exist in ER/Golgi mannosidases.
  52. ROLE OF CALSEQUESTRIN AND ITS PHOSPHORYLATION IN HEART
    Steven Cala; Fiscal Year: 2006
    ..Comparing frozen tissue samples of control and heart failure, we will determine whether triadin-1 glycans are a so defective, and whether differences exist in ER/Golgi mannosidases.
  53. Mannosidases in Glycoprotein Biosynthesis & Catabolism
    Kelley Moremen; Fiscal Year: 2004
    ..We have focused on the cx-mannosidases in the N-glycan pathway since they act as committed steps in the synthesis of complex oligosaccharides by ..
  54. Mannosidases in Glycoprotein Biosynthesis & Catabolism
    Kelley Moremen; Fiscal Year: 2003
    ..We have focused on the cx-mannosidases in the N-glycan pathway since they act as committed steps in the synthesis of complex oligosaccharides by ..
  55. Mannosidases in Glycoprotein Biosynthesis & Catabolism
    Kelley Moremen; Fiscal Year: 2005
    ..We have focused on the cx-mannosidases in the N-glycan pathway since they act as committed steps in the synthesis of complex oligosaccharides by ..
  56. Mannosidases in Glycoprotein Biosynthesis & Catabolism
    Kelley Moremen; Fiscal Year: 2001
    ..We have focused on the cx-mannosidases in the N-glycan pathway since they act as committed steps in the synthesis of complex oligosaccharides by ..
  57. Mannosidases in Glycoprotein Biosynthesis & Catabolism
    Kelley Moremen; Fiscal Year: 2002
    ..We have focused on the cx-mannosidases in the N-glycan pathway since they act as committed steps in the synthesis of complex oligosaccharides by ..
  58. SYNTHESIS OF INHIBITORS & AFFINITY LIGANDS FOR PROCESSING MANNOSIDASES
    Kelley Moremen; Fiscal Year: 2000
    ..The expression of GlcNAc-T V mRNA has been described during mouse development using in situ hybridization techniques. Large expression levels were observed in brain and nerve tissue and in cells with high motility. ..
  59. SYNTHESIS OF INHIBITORS & AFFINITY LIGANDS FOR PROCESSING MANNOSIDASES
    Kelley Moremen; Fiscal Year: 2002
    ..The expression of GlcNAc-T V mRNA has been described during mouse development using in situ hybridization techniques. Large expression levels were observed in brain and nerve tissue and in cells with high motility. ..
  60. SYNTHESIS OF INHIBITORS & AFFINITY LIGANDS FOR PROCESSING MANNOSIDASES
    Kelley Moremen; Fiscal Year: 2001
    ..The expression of GlcNAc-T V mRNA has been described during mouse development using in situ hybridization techniques. Large expression levels were observed in brain and nerve tissue and in cells with high motility. ..
  61. TRANSGENIC CHARACTERIZATION OF CLASS II MAMMALIAN MANNOSIDASES
    Kelley Moremen; Fiscal Year: 2000
    ....
  62. TRANSGENIC CHARACTERIZATION OF CLASS II MAMMALIAN MANNOSIDASES
    Kelley Moremen; Fiscal Year: 2002
    ....
  63. TRANSGENIC CHARACTERIZATION OF CLASS II MAMMALIAN MANNOSIDASES
    Kelley Moremen; Fiscal Year: 2001
    ....
  64. CLONING & CHARACTERIZING CLASS II MANNOSIDASES IN MAMMALIAN SYSTEMS
    Kelley Moremen; Fiscal Year: 2001
    A variety of modified alkaloid compounds are being synthesized for testing of their ability to inhibit processing and catabolic mannosidases as well as of their use as affinity ligands for purification of recombinant enzymes.