beta galactosidase

Summary

Summary: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS GM1.

Top Publications

  1. ncbi Three-dimensional structure of beta-galactosidase from E. coli
    R H Jacobson
    Institute of Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene 97403
    Nature 369:761-6. 1994
  2. ncbi Quantification of beta-galactosidase activity after non-viral transfection in vivo
    Bernd H Zinselmeyer
    Cancer Research, UK Department of Medical Oncology, Beatson Laboratories, University of Glasgow, G61 1BD, Glasgow, UK
    J Control Release 91:201-8. 2003
  3. pmc Ink4a/Arf expression is a biomarker of aging
    Janakiraman Krishnamurthy
    Department of Medicine, The Lineberger Comprehensive Cancer Center, The University of North Carolina School of Medicine, Chapel Hill, North Carolina, 27599 7295, USA
    J Clin Invest 114:1299-307. 2004
  4. ncbi Sirt1 inhibitor, Sirtinol, induces senescence-like growth arrest with attenuated Ras-MAPK signaling in human cancer cells
    H Ota
    Department of Anesthesia and Critical Care, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Oncogene 25:176-85. 2006
  5. ncbi Senescence-associated (beta)-galactosidase reflects an increase in lysosomal mass during replicative ageing of human endothelial cells
    D J Kurz
    Cell Biology Group, Centre for Cardiovascular Biology and Medicine, Department of Medicine, Royal Free and University College Medical School, University College London, London WC1E 6JJ, UK
    J Cell Sci 113:3613-22. 2000
  6. ncbi Senescence-associated beta-galactosidase is lysosomal beta-galactosidase
    Bo Yun Lee
    Department of Life Science, University of Seoul, Dongdaemungu, Jeonnongdong, Seoul, Korea 130 743
    Aging Cell 5:187-95. 2006
  7. ncbi Stochastic protein expression in individual cells at the single molecule level
    Long Cai
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    Nature 440:358-62. 2006
  8. ncbi A reporter gene system for the hyperthermophilic archaeon Sulfolobus solfataricus based on a selectable and integrative shuttle vector
    Melanie Jonuscheit
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstr 10, Germany
    Mol Microbiol 48:1241-52. 2003
  9. ncbi Irreversible cellular senescence induced by prolonged exposure to H2O2 involves DNA-damage-and-repair genes and telomere shortening
    Jianming Duan
    Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100083, China
    Int J Biochem Cell Biol 37:1407-20. 2005
  10. pmc Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells
    B P Zambrowicz
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 94:3789-94. 1997

Research Grants

  1. BONE MARROW TRANSPLANTION AND ATHEROSCLEROSIS
    MacRae Linton; Fiscal Year: 1999
  2. DnaK Protein Inhibitor Antimicrobials
    Michael Sturgess; Fiscal Year: 2003
  3. INTERLEUKIN-1 AND PERINATAL BRAIN INJURY
    Faye Silverstein; Fiscal Year: 1999
  4. CYTOMEGALOVIRUS DNA REPLICATION AND INVERSION
    Edward Mocarski; Fiscal Year: 1991
  5. CHEMICAL PATHOLOGY OF NEUROLOGICAL DISORDERS
    Kunihiko Suzuki; Fiscal Year: 1993
  6. CHEMICAL PATHOLOGY OF NEUROLOGICAL DISORDERS
    Kunihiko Suzuki; Fiscal Year: 1999
  7. CHEMICAL PATHOLOGY OF NEUROLOGICAL DISORDERS
    Kunihiko Suzuki; Fiscal Year: 1992
  8. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2006
  9. MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
    L Jeannine Brady; Fiscal Year: 2002
  10. MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
    ARNOLD BLEIWEIS; Fiscal Year: 2001

Detail Information

Publications206 found, 100 shown here

  1. ncbi Three-dimensional structure of beta-galactosidase from E. coli
    R H Jacobson
    Institute of Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene 97403
    Nature 369:761-6. 1994
    ..Non-crystallographic symmetry averaging proved to be a very powerful tool in the structure determination, as has been shown in other contexts...
  2. ncbi Quantification of beta-galactosidase activity after non-viral transfection in vivo
    Bernd H Zinselmeyer
    Cancer Research, UK Department of Medical Oncology, Beatson Laboratories, University of Glasgow, G61 1BD, Glasgow, UK
    J Control Release 91:201-8. 2003
    ..After intravenous administration of beta-galactosidase complexed with linear polyethylenimine (PEI, 22 kD) in mice, 134 mU g(-1) beta-galactosidase were detected in the lung, but only 2.9 mU g(-1) were found in the liver...
  3. pmc Ink4a/Arf expression is a biomarker of aging
    Janakiraman Krishnamurthy
    Department of Medicine, The Lineberger Comprehensive Cancer Center, The University of North Carolina School of Medicine, Chapel Hill, North Carolina, 27599 7295, USA
    J Clin Invest 114:1299-307. 2004
    ..These data suggest that expression of the Ink4a/Arf tumor suppressor locus is a robust biomarker, and possible effector, of mammalian aging...
  4. ncbi Sirt1 inhibitor, Sirtinol, induces senescence-like growth arrest with attenuated Ras-MAPK signaling in human cancer cells
    H Ota
    Department of Anesthesia and Critical Care, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Oncogene 25:176-85. 2006
    ..These results suggest that inhibitors for Sirt1 may have anticancer potential, and that impaired activation of Ras-MAPK pathway might take part in a senescence-like growth arrest program induced by Sirtinol...
  5. ncbi Senescence-associated (beta)-galactosidase reflects an increase in lysosomal mass during replicative ageing of human endothelial cells
    D J Kurz
    Cell Biology Group, Centre for Cardiovascular Biology and Medicine, Department of Medicine, Royal Free and University College Medical School, University College London, London WC1E 6JJ, UK
    J Cell Sci 113:3613-22. 2000
    ..These findings demonstrate that senescence-associated (beta)-galactosidase is a manifestation of residual lysosomal activity at a suboptimal pH, which becomes detectable due to the increased lysosomal content in senescent cells...
  6. ncbi Senescence-associated beta-galactosidase is lysosomal beta-galactosidase
    Bo Yun Lee
    Department of Life Science, University of Seoul, Dongdaemungu, Jeonnongdong, Seoul, Korea 130 743
    Aging Cell 5:187-95. 2006
    ..SA-beta-gal induction during senescence was due at least in part to increased expression of the lysosomal beta-galactosidase protein. These results also indicate that SA-beta-gal is not required for senescence...
  7. ncbi Stochastic protein expression in individual cells at the single molecule level
    Long Cai
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    Nature 440:358-62. 2006
    ..This microfluidic single cell assay opens up possibilities for system-wide characterization of the expression of these low copy number proteins...
  8. ncbi A reporter gene system for the hyperthermophilic archaeon Sulfolobus solfataricus based on a selectable and integrative shuttle vector
    Melanie Jonuscheit
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstr 10, Germany
    Mol Microbiol 48:1241-52. 2003
    ....
  9. ncbi Irreversible cellular senescence induced by prolonged exposure to H2O2 involves DNA-damage-and-repair genes and telomere shortening
    Jianming Duan
    Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100083, China
    Int J Biochem Cell Biol 37:1407-20. 2005
    ..Our findings indicate that several aging theories can be merged together by a common mechanism of oxidative damage, and that the level of oxidative DNA-damage-and-repair capacity may be exploited as reliable markers of cell senescence...
  10. pmc Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells
    B P Zambrowicz
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 94:3789-94. 1997
    ..This third transcript potentially encodes a novel protein of at least 505 amino acids that is conserved in humans and in Caenorhabditis elegans...
  11. pmc Expression of senescence-related genes in human corneal endothelial cells
    Zhenhua Song
    State Key Lab Cultivation Base, Shandong Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao, China
    Mol Vis 14:161-70. 2008
    ..To investigate the expression of p16(INK4a), p21(WAF1/CIP1), p27(KIP1), and p53 in human corneal endothelial cell (HCEC) senescence ex vivo at various donor ages...
  12. ncbi Neuroprotective effect of a CNTF-expressing lentiviral vector in the quinolinic acid rat model of Huntington's disease
    L P de Almeida
    Division of Surgical Research and Gene Therapy Center, Lausanne Medical School, Switzerland
    Neurobiol Dis 8:433-46. 2001
    ..These results further establish that lentiviruses may represent an efficient gene delivery system for the screening of therapeutic molecules in Huntington's disease...
  13. ncbi Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice
    G Friedrich
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030
    Genes Dev 5:1513-23. 1991
    ..Because no overt phenotype was detected in the remaining strains, these results suggest that a substantial proportion of mammalian genes identified by this approach are not essential for development...
  14. ncbi Identification and functional characterization of distinct critically important bone morphogenetic protein-specific response elements in the Id1 promoter
    Olexander Korchynskyi
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 277:4883-91. 2002
    ..Our results provide important new insights into how the BMP/Smad pathway can specifically activate target genes...
  15. ncbi Treatment of anaplastic thyroid carcinoma in vitro with a mutant vaccinia virus
    Shu Fu Lin
    Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Surgery 142:976-83; discussion 976-83. 2007
    ..Anaplastic thyroid carcinoma (ATC) is a fatal disease resistant to all conventional treatments. Novel therapies are needed to improve dismal outcomes...
  16. pmc Vascular bed-specific expression of an endothelial cell gene is programmed by the tissue microenvironment
    W C Aird
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02119, USA
    J Cell Biol 138:1117-24. 1997
    ..Taken together, the results suggest that the vWF gene is regulated by vascular bed-specific pathways in response to signals derived from the local microenvironment...
  17. pmc Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes
    Isabelle Ventre
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Centre National de la Recherche Scientifique, Institut de Biologie Structurale et Microbiologie, Unite Propre de Recherche 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Proc Natl Acad Sci U S A 103:171-6. 2006
    ....
  18. ncbi Synchronous activity of inhibitory networks in neocortex requires electrical synapses containing connexin36
    M R Deans
    Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
    Neuron 31:477-85. 2001
    ..However, the synchrony of these rhythms was weaker and more spatially restricted in the knockout. We conclude that electrical synapses containing Cx36 are critical for the generation of widespread, synchronous inhibitory activity...
  19. pmc Immunotherapy with dendritic cells directed against tumor antigens shared with normal host cells results in severe autoimmune disease
    B Ludewig
    Institute of Experimental Immunology, CH 8091 Zurich, Switzerland
    J Exp Med 191:795-804. 2000
    ..These results reveal the delicate balance between tumor immunity and autoimmunity and therefore point out important limitations for the use of not strictly tumor-specific antigens in antitumor vaccination with DCs...
  20. ncbi Inducible Cre-mediated control of gene expression in the murine gastrointestinal tract: effect of loss of beta-catenin
    Heather Ireland
    Department of Oncology, Cancer Research UK, Cambridge Institute of Medical Research, Wellcome Trust MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2XY, UK
    Gastroenterology 126:1236-46. 2004
    ....
  21. ncbi The spoIIIA operon of Bacillus subtilis defines a new temporal class of mother-cell-specific sporulation genes under the control of the sigma E form of RNA polymerase
    N Illing
    Sir William Dunn School of Pathology, University of Oxford, UK
    Mol Microbiol 5:1927-40. 1991
    ..Since the onset of spoIIID expression occurs about 10 minutes after that of spoIIIA, spoIIIA expression is transient. Thus spoIIIA defines a third temporal class of gene controlled by the sigma E form of RNA polymerase...
  22. ncbi Sirt1 modulates premature senescence-like phenotype in human endothelial cells
    Hidetaka Ota
    Department of Geriatric Medicine, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    J Mol Cell Cardiol 43:571-9. 2007
    ..Our data suggest that Sirt1 may exert protective effects against endothelial dysfunction by preventing stress-induced premature senescence and deranged expression of PAI-1 and eNOS...
  23. pmc Cold-adapted beta-galactosidase from the Antarctic psychrophile Pseudoalteromonas haloplanktis
    A Hoyoux
    Laboratory of Biochemistry, Institute of Chemistry, University of Liege, Sart-Tilman, B-4000 Liege, Belgium
    Appl Environ Microbiol 67:1529-35. 2001
    ..lactis, suggesting that the cold-adapted beta-galactosidase could be used to hydrolyze lactose in dairy products processed in refrigerated plants...
  24. ncbi Extension of life-span by introduction of telomerase into normal human cells
    A G Bodnar
    Geron Corporation, Menlo Park, CA 94025, USA
    Science 279:349-52. 1998
    ..The ability to maintain normal human cells in a phenotypically youthful state could have important applications in research and medicine...
  25. pmc Senescence associated beta galactosidase activity in human retinal pigment epithelial cells exposed to mild hyperoxia in vitro
    Shigeru Honda
    Department of Ophthalmology, University of California, Davis, CA, USA
    Br J Ophthalmol 86:159-62. 2002
    ..To determine if mild hyperoxia induces senescence in retinal pigment epithelium (RPE) cells in vitro...
  26. ncbi Beta galactosidase enzyme fragment complementation as a novel technology for high throughput screening
    Richard M Eglen
    Research and Development, DiscoveRx Corp, 42501 Albrae St, Suite 100, Fremont, CA 94538, USA
    Comb Chem High Throughput Screen 6:381-7. 2003
    In this review, the applications of beta galactosidase complementation are described. alpha Complementation is a naturally occurring process in bacteria and in engineered cells, and can also occur in eukaryotic cells...
  27. ncbi Thermal denaturation of beta-galactosidase and of two site-specific mutants
    R A Edwards
    Division of Biochemistry, Faculty of Science, University of Calgary, Alberta, Canada
    Biochemistry 29:11001-8. 1990
    ..Other effects result from changes in ligand binding, but the magnitude of the resulting changes in stability is not related to the strength of ligand binding in a simple fashion...
  28. ncbi kappa B-like motifs regulate the induction of immune genes in Drosophila
    Y Engstrom
    Department of Molecular Biology, Stockholm University, Sweden
    J Mol Biol 232:327-33. 1993
    ..Our data suggest a functional and evolutionary relationship between these insect immune response factors and the mammalian NF-kappa B...
  29. ncbi Determination of the roles of Glu-461 in beta-galactosidase (Escherichia coli) using site-specific mutagenesis
    C G Cupples
    Molecular Biology Institute, University of California, Los Angeles 90024
    J Biol Chem 265:5512-8. 1990
    ..The enzyme with His substituted for Glu-461 had the highest kappa 3 value. This is probably a result of the formation of a covalent bond between His and the galactosyl part of the substrate...
  30. pmc Caspase-mediated processing of the Drosophila NF-kappaB factor Relish
    Svenja Stoven
    Umea Centre for Molecular Pathogenesis, Umea University, S 901 87 Umea, Sweden
    Proc Natl Acad Sci U S A 100:5991-6. 2003
    ..Finally, an N-terminal serine-rich region in Relish and the PEST domain were found to negatively regulate Relish activation...
  31. pmc Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice
    X Mao
    Division of Hematology Oncology, Department of Pediatrics, Children s Hospital and the Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 96:5037-42. 1999
    ..This improved reporter strain should facilitate monitoring in vivo Cre-mediated excision events in a variety of experimental contexts...
  32. ncbi A selectable bifunctional beta-galactosidase::phleomycin-resistance fusion protein as a potential marker for eukaryotic cells
    M Baron
    Laboratoire de Microbiologie, Universite Paul Sabatier, Toulouse, France
    Gene 114:239-43. 1992
    ..This system appears to be a potentially useful tool for the direct selection of transformants, and the evaluation of gene expression and regulation in a wide variety of prokaryotic and eukaryotic hosts...
  33. ncbi Glu-537, not Glu-461, is the nucleophile in the active site of (lac Z) beta-galactosidase from Escherichia coli
    J C Gebler
    Department of Chemistry, University of British Columbia, Vancouver, Canada
    J Biol Chem 267:11126-30. 1992
    ..and Legler, G. (1984) Eur. J. Biochem. 138, 527-531) on the basis of affinity labeling studies with conduritol C cis-epoxide. A role for glutamic acid residue 461 as the acid/base catalyst is proposed and justified...
  34. ncbi Endothelial cell senescence in human atherosclerosis: role of telomere in endothelial dysfunction
    Tohru Minamino
    Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, Chiba, Japan
    Circulation 105:1541-4. 2002
    ..However, whether senescent vascular cells are present in the vasculature and contribute to the pathogenesis of atherosclerosis remains unclear...
  35. ncbi Differential effects of genotoxic stress on both concurrent body growth and gradual senescence in the adult zebrafish
    Stephanie B Tsai
    Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA, USA
    Aging Cell 6:209-24. 2007
    ..Moreover, our present findings indicate that genotoxic DNA damage may play a role as a rate-limiting factor during the induction of senescence, but not in the inhibition of continuous, density-dependent growth in adult zebrafish...
  36. ncbi Markers of senescence?
    Philip J Coates
    J Pathol 196:371-3. 2002
    ....
  37. ncbi Eukaryotic promoters can direct protein synthesis in Gram-negative bacteria
    Sylvie Goussard
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    J Mol Microbiol Biotechnol 6:211-8. 2003
    ..These observations should incite to exert caution when interpreting data on the DNA transfer from bacteria to mammallian cells when the genes of interest are under the control of the PCMV or PRSV promoter...
  38. ncbi In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates
    I Matsumura
    Institute of Cellular and Molecular Biology, ICMB A4800 MBB 3 424, University of Texas, 26th and Speedway, Austin, TX, 78712, USA
    J Mol Biol 305:331-9. 2001
    ..These results are consistent with the "patchwork" hypothesis, which postulates that modern enzymes diverged from ancestors with broad specificity...
  39. ncbi Central role of the proteasome in senescence and survival of human fibroblasts: induction of a senescence-like phenotype upon its inhibition and resistance to stress upon its activation
    Niki Chondrogianni
    National Hellenic Research Foundation, Institute of Biological Research and Biotechnology, 48 Vas Constantinou Ave, Athens 11635, Greece
    J Biol Chem 278:28026-37. 2003
    ..In summary these data demonstrate the central role of the proteasome during cellular senescence and survival as well as provide insights toward a better understanding of proteasome regulation...
  40. ncbi In vivo imaging of beta-galactosidase activity using far red fluorescent switch
    Ching Hsuan Tung
    Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Cancer Res 64:1579-83. 2004
    ..The results indicated that in vivo real-time detection of beta-gal activity is possible by fluorescence imaging technology...
  41. pmc Novel oncolytic agent GLV-1h68 is effective against malignant pleural mesothelioma
    Kaitlyn J Kelly
    Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Hum Gene Ther 19:774-82. 2008
    ..GLV-1h68 was successfully used to treat MPM in vitro and in an orthotopic model (in vivo). These promising results warrant clinical investigation of GLV-1h68 as a novel agent in the treatment of MPM...
  42. pmc A high-throughput screen for synthetic riboswitches reveals mechanistic insights into their function
    Sean A Lynch
    Department of Chemistry and Center for Fundamental and Applied Molecular Evolution, Emory University, Atlanta, GA 30322, USA
    Chem Biol 14:173-84. 2007
    ..We anticipate that the screening method and design principles will be generally useful for creating functional synthetic riboswitches...
  43. ncbi Loss of AP-2alpha impacts multiple aspects of ventral body wall development and closure
    Stephanie Brewer
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Dev Biol 267:399-417. 2004
    ..These findings provide a fundamental understanding of how AP-2alpha functions in the closure of the ventral body wall, as well as offer insight into related human birth defects...
  44. ncbi Regulation of cellular senescence and p16(INK4a) expression by Id1 and E47 proteins in human diploid fibroblast
    Wenjie Zheng
    Department of Biochemistry and Molecular Biology, Peking University, Health Science Center, 38 Xueyuan Road, Beijing 100083, People s Republic of China
    J Biol Chem 279:31524-32. 2004
    ....
  45. ncbi Cloning, expression, and purification of a recombinant cold-adapted beta-galactosidase from antarctic bacterium Pseudoalteromonas sp. 22b
    Hubert Cieśliński
    Department of Microbiology, Gdansk University of Technology, ul G Narutowicza 11 12, 80 952 Gdansk, Poland
    Protein Expr Purif 39:27-34. 2005
    ..Because of its attributes, the recombinant Pseudoalteromonas sp. 22b beta-galactosidase could be applied at refrigeration temperatures for production of lactose-reduced dairy products...
  46. ncbi Novel NMR approach to assessing gene transfection: 4-fluoro-2-nitrophenyl-beta-D-galactopyranoside as a prototype reporter molecule for beta-galactosidase
    Weina Cui
    Department of Radiology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9058, USA
    Magn Reson Med 51:616-20. 2004
    ..Molecular and 19F NMR characteristics of PFONPG in solution, blood, and prostate tumor cells are presented. This prototype molecule facilitates a novel approach for assaying gene activity in vivo...
  47. pmc Transcriptional regulation of sitABCD of Salmonella enterica serovar Typhimurium by MntR and Fur
    Jack S Ikeda
    Department of Microbiology, University of Illinois, B103 CLSL, 601 S Goodwin Ave, Urbana, IL 61801, USA
    J Bacteriol 187:912-22. 2005
    ....
  48. ncbi Papaya beta-galactosidase/galactanase isoforms in differential cell wall hydrolysis and fruit softening during ripening
    Hamid Lazan
    Faculty of Science and Technology, School of Biosciences and Biotechnology, University Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia
    Plant Physiol Biochem 42:847-53. 2004
    ..Collectively, the results support the case for a functional relevance of the papaya enzymes in softening related changes during ripening...
  49. ncbi Cooperative effects by the initiation codon and its flanking regions on translation initiation
    C M Stenström
    Department of Microbiology, Stockholm University, S 106 91, Stockholm, Sweden
    Gene 273:259-65. 2001
    ..The otherwise weak initiation codon UUG, but not GUG, was comparable with AUG in strength, if placed in the context of two of the DRs. The +2 codon was one, but not the only, determinant for this unexpectedly high efficiency of UUG...
  50. pmc SIRT1 overexpression antagonizes cellular senescence with activated ERK/S6k1 signaling in human diploid fibroblasts
    Jing Huang
    Peking University Research Center on Aging, Beijing, People s Republic of China
    PLoS ONE 3:e1710. 2008
    ..These findings suggested that SIRT1-promoted cell proliferation and antagonized cellular senescence in human diploid fibroblasts may be, in part, via the activation of ERK/ S6K1 signaling...
  51. ncbi Beta galactosidase complementation: a cell-based luminescent assay platform for drug discovery
    Keith R Olson
    DiscoveRx Corp, Fremont, CA, USA
    Assay Drug Dev Technol 5:137-44. 2007
    ..This review describes the various uses of this flexible technology to cell-based assay development...
  52. ncbi Mature hepatocytes are the source of small hepatocyte-like progenitor cells in the retrorsine model of liver injury
    Audrey Avril
    Biothérapies Hépatiques, CIC INSERM 00 04, CHU Hotel Dieu, 44093 Nantes Cedex 01, France
    J Hepatol 41:737-43. 2004
    ..Our aim was to test whether SHPCs could originate from mature hepatocytes...
  53. ncbi Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose
    A L Rojas
    Instituto de Fisica de Sao Carlos, Universidade de Sao Paulo, Av Trabalhador Sao Carlense 400, CEP 13560 970 São Carlos, SP, Brazil
    J Mol Biol 343:1281-92. 2004
    ..Penicillium sp. beta-galactosidase is a glycoprotein containing seven N-linked oligosaccharide chains and is the only structure of a glycosylated beta-galactosidase described to date...
  54. pmc A double reporter assay for detecting changes in the ratio of spliced and unspliced mRNA in mammalian cells
    Md Talat Nasim
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, UK
    Nucleic Acids Res 30:e109. 2002
    ..This provides a rapid and convenient assay for the effects on splicing efficiency of trans-acting factors or of alterations in the sequences of the intron and surrounding exon sequences...
  55. doi The potential action of galactose as a "chemical chaperone": increase of beta galactosidase activity in fibroblasts from an adult GM1-gangliosidosis patient
    Anna Caciotti
    Metabolic and Muscular Unit, Clinic of Pediatric Neurology, AOU Meyer, Florence, Italy
    Eur J Paediatr Neurol 13:160-4. 2009
    ..Protein misfolding in lysosomal defects may have the potential to be corrected by chemical chaperones: in vitro and clinical approaches are being investigated...
  56. ncbi Analyzing mRNA-protein complexes using a yeast three-hybrid system
    David S Bernstein
    Department of Biochemistry, University of Wisconsin Madison, 53706, USA
    Methods 26:123-41. 2002
    ..Multicomponent complexes containing more than one protein can be detected, identified, and analyzed. We describe the method and how to use it, and discuss applications that bear particularly on eukaryotic mRNAs...
  57. ncbi Construction of a flocculent Saccharomyces cerevisiae strain secreting high levels of Aspergillus niger beta-galactosidase
    L Domingues
    Centro de Engenharia Biológica IBQF, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Appl Microbiol Biotechnol 58:645-50. 2002
    ..Moreover, the use of flocculating yeast cells allows for enzyme production with high productivity in continuous fermentation systems with facilitated downstream processing...
  58. ncbi Beta-galactosidase from a cold-adapted bacterium: purification, characterization and application for lactose hydrolysis
    S Fernandes
    Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Sweden
    Appl Microbiol Biotechnol 58:313-21. 2002
    ..The enzyme could be immobilized to Sepharose via different chemistries with 60-70% retention of activity. The immobilized enzyme was more stable and its ability to hydrolyze lactose was similar to that of the soluble enzyme...
  59. ncbi Purification and characterization of a beta-galactosidase from peach (Prunus persica)
    Dong Hoon Lee
    Department of Horticulture, Yeungnam University, Gyeongsan 712 749, Korea
    Mol Cells 15:68-74. 2003
    ..Therefore, enzymatic, immunological, and molecular evidences in this study indicate that the purified 42-kDa protein is a peach beta-galactosidase...
  60. ncbi Expression of beta-galactosidase and beta-xylosidase genes during microspore and pollen development
    Petra Hrubá
    Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Pernikarce 15, 160 00, Prague 6, Czech Republic
    Planta 220:931-40. 2005
    ....
  61. ncbi Overexpression and functional analysis of cold-active beta-galactosidase from Arthrobacter psychrolactophilus strain F2
    Tomoyuki Nakagawa
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099 2493, Japan
    Protein Expr Purif 54:295-9. 2007
    ..We conclude that rBglAp is a cold-active enzyme that is extremely heat labile and has significant potential application to the food industry...
  62. ncbi The role of chondrocyte senescence in the pathogenesis of osteoarthritis and in limiting cartilage repair
    James A Martin
    Department of Orthopaedics, University of Iowa College of Medicine, Iowa City 52242, USA
    J Bone Joint Surg Am 85:106-10. 2003
    ..These observations led us to hypothesize that progressive cell senescence decreases the ability of chondrocytes to maintain and to restore articular cartilage...
  63. ncbi 19F-NMR detection of lacZ gene expression via the enzymic hydrolysis of 2-fluoro-4-nitrophenyl beta-D-galactopyranoside in vivo in PC3 prostate tumor xenografts in the mouse
    Li Liu
    Department of Radiology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, Texas 75390 9058, USA
    FASEB J 21:2014-9. 2007
    ..More importantly, we demonstrate for the first time the ability to differentiate wild-type and lacZ-expressing prostate tumor xenografts in mice using this approach...
  64. ncbi Luminescent imaging of beta-galactosidase activity in living subjects using sequential reporter-enzyme luminescence
    Thomas S Wehrman
    Baxter Laboratory in Genetic Pharmacology, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nat Methods 3:295-301. 2006
    ..Thus, coupling the properties of FLuc to the advantages of beta-gal permits bioluminescent imaging applications that previously were not possible...
  65. ncbi Synthesis and evaluation of novel fluorogenic substrates for the detection of bacterial beta-galactosidase
    K F Chilvers
    Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, UK
    J Appl Microbiol 91:1118-30. 2001
    ....
  66. ncbi Endless versatility in the biotechnological applications of Kluyveromyces LAC genes
    Marta Rubio-Texeira
    68 541, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 02139, USA
    Biotechnol Adv 24:212-25. 2006
    ..Emphasis is also made on the potential that some of the latest technologies, such as the generation of transgenics, will have for a further benefit in the use of these and related genes...
  67. pmc FlhA influences Bacillus thuringiensis PlcR-regulated gene transcription, protein production, and virulence
    Laurent Bouillaut
    Unité Génétique Microbienne et Environnement, INRA, La Minière, 78285 Guyancourt Cedex, France
    Appl Environ Microbiol 71:8903-10. 2005
    ..The decrease in virulence may be due to both a lack of flagella and a lower production of secreted factors. Hence, FlhA appears to be an essential virulence factor with a pleiotropic role...
  68. ncbi Cloning of a Cicer arietinum beta-galactosidase with pectin-degrading function
    Rocío Esteban
    Dpto de Fisiología Vegetal Facultad de Biología, Universidad de Salamanca, Centro Hispano Luso de Investigaciones Agrarias, Pza Doctores de la Reina s n, Salamanca 37007, Spain
    Plant Cell Physiol 44:718-25. 2003
    ..It is suggested that the increase in the size of cell wall pores could permit access of other cell wall-modifying enzymes to their substrate...
  69. ncbi Progressive loss of SIRT1 with cell cycle withdrawal
    Tsutomu Sasaki
    Department of Neuroscience, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Aging Cell 5:413-22. 2006
    ..Thus, as mitotic activity ceases in mouse and human cells in the normal environment of the animal or in the culture dish, there is a concomitant decline in the level of SIRT1...
  70. ncbi Isolation of the promoter of a cotton beta-galactosidase gene (GhGal1) and its expression in transgenic tobacco plants
    Aimin Wu
    Laboratory of Molecular Biology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    Sci China C Life Sci 49:105-14. 2006
    ..These results indicated the temporal and spatial expression characterization of the GhGal1 promoter in transgenic tobacco plants and provided an important insight into the roles of GhGal1 in cotton fiber development...
  71. pmc Identification of new flagellar genes of Salmonella enterica serovar Typhimurium
    Jonathan Frye
    Sidney Kimmel Cancer Center, San Diego, California 92121, USA
    J Bacteriol 188:2233-43. 2006
    ..Null alleles in cheV, mcpA, mcpB, mcpC, and srfB did not affect motility, while deletion of yhjH did result in reduced motility compared to that of the wild type...
  72. ncbi Replication competence and senescence in central and peripheral human corneal endothelium
    Tatsuya Mimura
    Schepens Eye Research Institute and Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, USA
    Invest Ophthalmol Vis Sci 47:1387-96. 2006
    ..To compare replication competence and senescence in human corneal endothelial cells (HCECs) between the central and peripheral areas and between younger and older donors...
  73. ncbi Cold-active beta-galactosidase from Arthrobacter sp. C2-2 forms compact 660 kDa hexamers: crystal structure at 1.9A resolution
    Tereza Skalova
    Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovskeho nam 2, 1606 Praha 6, Czech Republic
    J Mol Biol 353:282-94. 2005
    ..This structure reveals new insights into the cold-adaptation mechanisms of enzymatic pathways of extremophiles...
  74. pmc A family of at least seven beta-galactosidase genes is expressed during tomato fruit development
    D L Smith
    Horticultural Crops Quality Laboratory, Agricultural Research Service, United States Department of Agriculture, Building 002, 10300 Baltimore Avenue, Beltsville, Maryland 20705 2350, USA
    Plant Physiol 123:1173-83. 2000
    ....
  75. ncbi Construction and characterization of a novel maltose inducible expression vector in Bacillus subtilis
    Yang Ming-Ming
    Institute of Biochemical Engineering, College of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, P R China
    Biotechnol Lett 28:1713-8. 2006
    ..16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two genes, bioA and vgb. This thus provided a potential expression system for cloned genes in B. subtilis...
  76. pmc Mutations in the Drosophila dTAK1 gene reveal a conserved function for MAPKKKs in the control of rel/NF-kappaB-dependent innate immune responses
    S Vidal
    Centre de Genetique Moleculaire, CNRS, 91198 Gif sur Yvette, France
    Genes Dev 15:1900-12. 2001
    ..Our genetic demonstration of dTAK1's role in the regulation of Drosophila antimicrobial peptide gene expression suggests an evolutionary conserved role for TAK1 in the activation of Rel/NF-kappaB-mediated host defense reactions...
  77. ncbi Calcium alginate entrapped preparations of Aspergillus oryzae beta galactosidase: its stability and applications in the hydrolysis of lactose
    Toshiba Haider
    Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, UP, India
    Int J Biol Macromol 41:72-80. 2007
    Insoluble concanavalin A-beta galactosidase complex was obtained by using jack bean extract and this complex was crosslinked with glutaraldehyde, in order to maintain the integrity of complex in the presence of its substrate or products...
  78. ncbi The zebrafish as a vertebrate model of functional aging and very gradual senescence
    Shuji Kishi
    Department of Cancer Biology, Dana Farber Cancer Institute and Harvard Medical School, 44 Binney Street, Boston, MA 02115 6084, USA
    Exp Gerontol 38:777-86. 2003
    ....
  79. ncbi Antisense suppression of a beta-galactosidase gene (TB G6) in tomato increases fruit cracking
    Edgar Moctezuma
    Produce Quality and Safety Laboratory, USDA ARS, Building 002, Henry A Wallace Beltsville Area Research Center, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
    J Exp Bot 54:2025-33. 2003
    ..Although the exact function of the TBG6 product is still unknown, these results implicate an important role for this enzyme in early fruit growth and development in tomato...
  80. ncbi Superoxide dismutase 1 knock-down induces senescence in human fibroblasts
    Gil Blander
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 278:38966-9. 2003
    ..Together, these findings support the notion that in normal human cells the SOD1 protein may play a role in the regulation of cellular lifespan by p53 and may also regulate the death signals in cancer cells...
  81. ncbi Fluctuations in transcription factor binding can explain the graded and binary responses observed in inducible gene expression
    Jason R Pirone
    Biomathematics Graduate Program and Department of Environmental and Molecular Toxicology, Campus Box 8203, North Carolina State University, Raleigh, NC 27695 8203, USA
    J Theor Biol 226:111-21. 2004
    ..Our modeling results are in good qualitative agreement with the experimental data, and illustrate how the binary and graded responses can stem from the same underlying mechanism...
  82. ncbi Development of improved Sindbis virus-based DNA expression vector
    Ryuya Yamanaka
    Clinical Gene Therapy Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA
    DNA Cell Biol 23:75-80. 2004
    ..We have demonstrated a high-level expression of the exogenous beta-galactosidase gene from the pSin-SV40-HDV-SV40pA construct using a Sindbis replication system...
  83. doi Evaluation of promoter strength in mouse and rat primary hepatocytes using adenovirus vectors
    Eri Arita
    Laboratory of Bio Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan
    Eur J Pharm Biopharm 70:1-6. 2008
    ..These findings should help in the construction of adenovirus vectors for expressing genes of interest in rodent primary cultured hepatocytes...
  84. ncbi Differences in the carriage and the ability to utilize the serotype associated virulence plasmid in strains of Salmonella enterica serotype Typhimurium investigated by use of a self-transferable virulence plasmid, pOG669
    John E Olsen
    Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Stigbøjlen 4, DK 1870 Frederiksberg C, Denmark
    Microb Pathog 36:337-47. 2004
    ..An internal fragment of the rpoS gene was sequenced in three strains. These all showed an identical sequence to a published S. typhimurium rpoS gene...
  85. ncbi Ad-mTERT-delta19, a conditional replication-competent adenovirus driven by the human telomerase promoter, selectively replicates in and elicits cytopathic effect in a cancer cell-specific manner
    Eunhee Kim
    BK21 Project for Medical Sciences, Institute for Cancer Research, Yonsei Cancer Center, Yonsei University College of Medicine, Seoul, 120 752, Korea
    Hum Gene Ther 14:1415-28. 2003
    ....
  86. ncbi Construction of a multiframe vector to express coding sequences in Escherichia coli
    V Dominguez-Martinez
    Facultad de Ciencias Biológicas e Instituto, Salud Pública, Universidad Veracruzana, Xalapa, Ver, Mexico
    Can J Microbiol 47:72-6. 2001
    ..In one case, levels of beta-galactosidase activity depended largely on the N-terminus of the translation products. We conclude that pMFV7 may be useful for expressing coding sequences regardless of their reading frame...
  87. ncbi An improved beta-galactosidase reporter gene
    D S Anson
    Department of Chemical Pathology, Women s and Children s Hospital, 72 King William Road, North Adelaide, SA 5006, Australia
    J Biotechnol 108:17-30. 2004
    ..coli gene sequence. A variant of the codon-optimised beta-galactosidase gene sequence that includes an effective nuclear localisation signal was also made...
  88. ncbi BACE (beta-secretase) modulates the processing of APLP2 in vivo
    L Pastorino
    Department of Psychiatry, Mount Sinai School of Medicine, New York, NY 10029, USA
    Mol Cell Neurosci 25:642-9. 2004
    ..Overexpression of BACE in cultured cells led to increased APLP2 processing. Our results strongly suggest that BACE is a neuronal protein that modulates the processing of both APP and APLP2...
  89. doi Using thermal energy produced by irradiation of Mn-Zn ferrite magnetic nanoparticles (MZF-NPs) for heat-inducible gene expression
    Qiu sha Tang
    School of Clinical Medical Science, Southeast University, Nanjing 210009, China
    Biomaterials 29:2673-9. 2008
    ..This novel system made use of the relative low Curie point of MZF-NPs to control the in vivo hyperthermia temperature and therefore acquired safe and effective heat-inducible transgene expression...
  90. pmc A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor
    Lisa Rickman
    Division of Mycobacterial Research, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Mol Microbiol 56:1274-86. 2005
    ..In summary, it has been shown that Rv3676 is a direct regulator of rpfA expression, and because rpfA codes for a resuscitation promoting factor this may implicate Rv3676 in reactivation of dormant M. tuberculosis infections...
  91. ncbi Characterization of tetracycline-inducible bitransgenic Krt12rtTA/+/tet-O-LacZ mice
    Tai ichiro Chikama
    Department of Ophthahlmology, University of Cincinnati, Ohio 45267, USA
    Invest Ophthalmol Vis Sci 46:1966-72. 2005
    ..To prepare binary transgenic mouse lines that overexpress reporter genes in a corneal-epithelium-specific manner when induced by doxycycline...
  92. ncbi Conditional mutagenesis of the murine serum response factor gene blocks cardiogenesis and the transcription of downstream gene targets
    Zhiyv Niu
    Center for Cardiovascular Development, Division of Cardiovascular Sciences, Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, 77030, USA
    J Biol Chem 280:32531-8. 2005
    ....
  93. ncbi Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability
    Hoang Duc Nguyen
    Institute of Genetics, University of Bayreuth, D 95440 Bayreuth, Germany Vietnam National University Ho Chi Minh City, College of Natural Sciences, Faculty of Biology, Ho Chi Minh City, Viet Nam
    Plasmid 54:241-8. 2005
    ..The versatility of these expression vectors was demonstrated by fusing their promoters to a reporter gene and by overexpression of the HtpG protein with three of them. All recombinant vectors exhibited full structural stability...
  94. pmc Identification of a crucial site for synoviolin expression
    Kaneyuki Tsuchimochi
    Department of Genomic Science, Institute of Medical Science, St Marianna University School of Medicine, Kawasaki, Kanagawa, Japan
    Mol Cell Biol 25:7344-56. 2005
    ..Our results suggest that transcriptional regulation of synoviolin via EBS-1 plays an important role in cellular homeostasis. Our study provides novel insight into the transcriptional regulation for cellular homeostasis...
  95. ncbi Optimization of transfection mediated by calcium phosphate for plasmid rAAV-LacZ (recombinant adeno-associated virus-beta-galactosidase reporter gene) production in suspension-cultured HEK-293 (human embryonic kidney 293) cells
    Lei Feng
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People s Republic of China
    Biotechnol Appl Biochem 46:127-35. 2007
    ..g. (vector genomes)/60 ml of medium respectively, is promising for the technique's application in the large-scale production of rAAV...
  96. ncbi The p53-family members p63 and p73 inhibit insulin-like growth factor-I receptor gene expression in colon cancer cells
    Irit Nahor
    Department of Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Israel
    Growth Horm IGF Res 15:388-96. 2005
    ..In summary, we have identified the IGF-IR gene as a novel downstream target for p63/p73 action...
  97. pmc A spreadable, non-integrative and high copy number shuttle vector for Sulfolobus solfataricus based on the genetic element pSSVx from Sulfolobus islandicus
    Tiziana Aucelli
    Istituto di Biochimica delle Proteine, Via P Castellino 111, 80131 CNR, Napoli, Italy
    Nucleic Acids Res 34:e114. 2006
    ..Complementation of the beta-galactosidase deficiency in a deletion mutant strain of S.solfataricus demonstrated that lacS gene was an efficient marker for selection of single transformants on solid minimal lactose medium...
  98. ncbi Localization of the endothelin-B receptor using a transgenic approach
    Adele Gordon
    Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK
    J Cardiovasc Pharmacol 44:S144-6. 2004
    ..These mice express beta- galactosidase wherever endothelin-B is expressed and therefore provide a precise in-vivo localization profile of endothelin-B expression...
  99. ncbi A simple system for converting lacZ to gfp reporter fusions in diverse bacteria
    Mark Goulian
    Department of Physics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Gene 372:219-26. 2006
    ..Green fluorescent protein expression in the new recombinants reflected the beta galactosidase expression in the parent strains...
  100. ncbi Transcriptional activity of the paired-like homeodomain proteins CHX10 and VSX1
    Kimberley M Dorval
    Toronto Western Research Institute, University Health Network Program, University of Toronto, Toronto, Ontario M5T 2S8, Canada
    J Biol Chem 280:10100-8. 2005
    ..Therefore, CHX10 and VSX1 may control retinal bipolar cell specification or differentiation by repressing genes required for the development of other cell types...
  101. ncbi Semliki Forest virus is an efficient and selective vector for gene delivery in infarcted rat heart
    Annemarieke E Loot
    Department of Clinical Pharmacology, Molecular Virology Section, University of Groningen, The Netherlands
    J Mol Cell Cardiol 37:137-42. 2004
    ..In conclusion, intracoronarily delivered SFV has a favourable distribution pattern, showing expression of the transgene restricted to the heart...

Research Grants69

  1. BONE MARROW TRANSPLANTION AND ATHEROSCLEROSIS
    MacRae Linton; Fiscal Year: 1999
    ..In addition, the transplantation of bone marrow from mice with the normal apoE gene into apo E deficient mice will be tested as a means of promoting regression of atherosclerosis in apoE deficient mice. ..
  2. DnaK Protein Inhibitor Antimicrobials
    Michael Sturgess; Fiscal Year: 2003
    ..abstract_text> ..
  3. INTERLEUKIN-1 AND PERINATAL BRAIN INJURY
    Faye Silverstein; Fiscal Year: 1999
    ....
  4. CYTOMEGALOVIRUS DNA REPLICATION AND INVERSION
    Edward Mocarski; Fiscal Year: 1991
    ..CMV insertion mutants will be constructed using E. coli beta galactosidase as a genetic marker...
  5. CHEMICAL PATHOLOGY OF NEUROLOGICAL DISORDERS
    Kunihiko Suzuki; Fiscal Year: 1993
    ....
  6. CHEMICAL PATHOLOGY OF NEUROLOGICAL DISORDERS
    Kunihiko Suzuki; Fiscal Year: 1999
    ....
  7. CHEMICAL PATHOLOGY OF NEUROLOGICAL DISORDERS
    Kunihiko Suzuki; Fiscal Year: 1992
    ..Furthermore, it is conceivable that genetic status resembling Gaucher disease might exist due to abnormality of this factor. Such a possibility will be tested when the factor is adequately characterized...
  8. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2006
    ..abstract_text> ..
  9. MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
    L Jeannine Brady; Fiscal Year: 2002
    ....
  10. MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
    ARNOLD BLEIWEIS; Fiscal Year: 2001
    ....
  11. Genesis of Liver Carcinomas with Oval Cell Traits
    Douglas Hixson; Fiscal Year: 2005
    ..By elucidating the role of oval cells in liver carcinogenesis, the results from these studies will provide valuable information that can be translated into new strategies for the diagnosis, treatment and prognosis of HCC. ..
  12. CHEMICAL PATHOLOGY OF NEUROLOGICAL DISORDERS
    Kunihiko Suzuki; Fiscal Year: 2001
    ..These point mutations are known in sap B and C in humans causing clinical diseases due to deficiency of the respective activator proteins without affecting processing of other domains. ..
  13. PTEN TUMOR SUPPRESSOR AND SIGNAL TRANSDUCTION
    Ramon Parsons; Fiscal Year: 2005
    ..Finally, PTEN heterozygous mice have tumors in multiple organ systems. They will breed PTEN+/- mice with IRS-2+/- mice to see if IRS-2 loss is able to attenuate the tumor phenotype. ..
  14. REPRODUCTIVE BIOCHEMISTRY OF TESTIS-SPECIFIC LDH-X
    Erwin Goldberg; Fiscal Year: 2002
    ..Ultimately, these data will permit us to fully realize the potential of gene therapy in alleviating male infertility and for fertility control. ..
  15. STRATEGIES FOR PRIMATE TRANSGENESIS
    Thaddeus Golos; Fiscal Year: 2003
    ..abstract_text> ..
  16. HETEROLOGOUS RECOMBINATION IN DSRNA PHAGE PHI 6
    Leonard Mindich; Fiscal Year: 1993
    ..It may be a major element in the evolution of RNA viruses. Phi6 is a model for the dsRNA viruses. Several members of the reoviridae, e.g. rotavirus and blue tongue virus, are the etiologic agents of important human and animal diseases...