phosphoprotein phosphatase

Summary

Summary: A group of enzymes removing the serine- or threonine-bound phosphate groups from a wide range of phosphoproteins, including a number of enzymes which have been phosphorylated under the action of a kinase. (Enzyme Nomenclature, 1992) EC 3.1.3.16.

Top Publications

  1. doi Regulators of PP2C phosphatase activity function as abscisic acid sensors
    Yue Ma
    Lehrstuhl fur Botanik, Technische Universitat Munchen, Am Hochanger 4, D 85354 Freising, Germany
    Science 324:1064-8. 2009
  2. ncbi Serine/threonine phosphatases: mechanism through structure
    Yigong Shi
    Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China
    Cell 139:468-84. 2009
  3. pmc The extended PP1 toolkit: designed to create specificity
    Mathieu Bollen
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, University of Leuven, B 3000 Leuven, Belgium
    Trends Biochem Sci 35:450-8. 2010
  4. pmc Regulated targeting of protein phosphatase 1 to the outer kinetochore by KNL1 opposes Aurora B kinase
    Dan Liu
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Biol 188:809-20. 2010
  5. doi Docking motif-guided mapping of the interactome of protein phosphatase-1
    Annick Hendrickx
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, Faculty of Medicine, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    Chem Biol 16:365-71. 2009
  6. doi From promiscuity to precision: protein phosphatases get a makeover
    David M Virshup
    Program in Cancer and Stem Cell Biology, Duke NUS Graduate Medical School Singapore, Singapore, Republic of Singapore
    Mol Cell 33:537-45. 2009
  7. doi WIP1 phosphatase at the crossroads of cancer and aging
    Xavier Le Guezennec
    Institute of Molecular and Cell Biology, Cell Cycle Control and Tumorigenesis Group, 61 Biopolis Drive, Proteos, Singapore
    Trends Biochem Sci 35:109-14. 2010
  8. pmc Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2alpha
    I Novoa
    Skirball Institute of Biomolecular Medicine, Department of Medicine, New York University School of Medicine, New York, NY 10016, USA
    J Cell Biol 153:1011-22. 2001
  9. ncbi Wip1 phosphatase modulates ATM-dependent signaling pathways
    Sathyavageeswaran Shreeram
    Institute of Molecular and Cell Biology, 61 Bioplis Drive, Proteos, 138673, Singapore
    Mol Cell 23:757-64. 2006
  10. ncbi Molecular aspects of seed dormancy
    Ruth Finkelstein
    Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, California 93106 9610, USA
    Annu Rev Plant Biol 59:387-415. 2008

Detail Information

Publications327 found, 100 shown here

  1. doi Regulators of PP2C phosphatase activity function as abscisic acid sensors
    Yue Ma
    Lehrstuhl fur Botanik, Technische Universitat Munchen, Am Hochanger 4, D 85354 Freising, Germany
    Science 324:1064-8. 2009
    ..Other RCARs also mediated ABA-dependent regulation of ABI1 and ABI2, consistent with a combinatorial assembly of receptor complexes...
  2. ncbi Serine/threonine phosphatases: mechanism through structure
    Yigong Shi
    Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China
    Cell 139:468-84. 2009
    ..Here, we discuss biochemical and structural investigations that advance the mechanistic understanding of the three major classes of PSPs, with a focus on PP2A...
  3. pmc The extended PP1 toolkit: designed to create specificity
    Mathieu Bollen
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, University of Leuven, B 3000 Leuven, Belgium
    Trends Biochem Sci 35:450-8. 2010
    ..These novel insights offer perspectives for the therapeutic targeting of PP1 by interfering with the binding of PIPs or substrates...
  4. pmc Regulated targeting of protein phosphatase 1 to the outer kinetochore by KNL1 opposes Aurora B kinase
    Dan Liu
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Biol 188:809-20. 2010
    ..In total, our results support a positive feedback mechanism by which Aurora B activity at kinetochores not only targets substrates directly, but also prevents localization of the opposing phosphatase...
  5. doi Docking motif-guided mapping of the interactome of protein phosphatase-1
    Annick Hendrickx
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, Faculty of Medicine, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    Chem Biol 16:365-71. 2009
    ..Notably, an interaction network linking PP1 interactors discloses a pleiotropic role of PP1 in cell polarity...
  6. doi From promiscuity to precision: protein phosphatases get a makeover
    David M Virshup
    Program in Cancer and Stem Cell Biology, Duke NUS Graduate Medical School Singapore, Singapore, Republic of Singapore
    Mol Cell 33:537-45. 2009
    ..As recent publications illustrate, these regulatory subunits confer specificity, selectivity, localization, and regulation on these important enzymes...
  7. doi WIP1 phosphatase at the crossroads of cancer and aging
    Xavier Le Guezennec
    Institute of Molecular and Cell Biology, Cell Cycle Control and Tumorigenesis Group, 61 Biopolis Drive, Proteos, Singapore
    Trends Biochem Sci 35:109-14. 2010
    ..These emerging functions of WIP1 make it a potent therapeutic target against cancer and aging...
  8. pmc Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2alpha
    I Novoa
    Skirball Institute of Biomolecular Medicine, Department of Medicine, New York University School of Medicine, New York, NY 10016, USA
    J Cell Biol 153:1011-22. 2001
    ....
  9. ncbi Wip1 phosphatase modulates ATM-dependent signaling pathways
    Sathyavageeswaran Shreeram
    Institute of Molecular and Cell Biology, 61 Bioplis Drive, Proteos, 138673, Singapore
    Mol Cell 23:757-64. 2006
    ..We propose that the Wip1 phosphatase is an integral component of an ATM-dependent signaling pathway...
  10. ncbi Molecular aspects of seed dormancy
    Ruth Finkelstein
    Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, California 93106 9610, USA
    Annu Rev Plant Biol 59:387-415. 2008
    ..The net result is a slightly heterogeneous response, thereby providing more temporal options for successful germination...
  11. ncbi PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms
    John Brognard
    Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093, USA
    Mol Cell 25:917-31. 2007
    ..Our data unveil a mechanism to selectively terminate Akt-signaling pathways through the differential inactivation of specific Akt isoforms by specific PHLPP isoforms...
  12. ncbi Regulation of protein kinase cascades by protein phosphatase 2A
    T A Millward
    Friedrich Miescher Institut, Maulbeerstrasse 66, CH 4058 Basel, Switzerland
    Trends Biochem Sci 24:186-91. 1999
    ..There is increasing evidence that PP2A activity is regulated by extracellular signals and during the cell cycle. Thus, PP2A is likely to play an important role in determining the activation kinetics of protein kinase cascades...
  13. ncbi PTG, a protein phosphatase 1-binding protein with a role in glycogen metabolism
    J A Printen
    Department of Physiology, University of Michigan School of Medicine, Ann Arbor, MI 48109, USA
    Science 275:1475-8. 1997
    ..These results suggest that PTG is critical for glycogen metabolism, possibly functioning as a molecular scaffold...
  14. ncbi Selective inhibition of a regulatory subunit of protein phosphatase 1 restores proteostasis
    Pavel Tsaytler
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Science 332:91-4. 2011
    ..This favored protein folding and thereby rescued cells from protein misfolding stress. Thus, regulatory subunits of phosphatases are drug targets, a property used here to restore proteostasis in stressed cells...
  15. ncbi Regulation of cell migration by the C2 domain of the tumor suppressor PTEN
    Myrto Raftopoulou
    Medical Research Council Laboratory for Molecular Cell Biology and Cell Biology Unit, Cancer Research UK Oncogene and Signal Transduction Group, University College London, Gower Street, London WC1E 6BT, UK
    Science 303:1179-81. 2004
    ..The ability of PTEN to control cell migration through its C2 domain is likely to be an important feature of its tumor suppressor activity...
  16. ncbi A two-component system that regulates an osmosensing MAP kinase cascade in yeast
    T Maeda
    Division of Tumor Immunology, Dana Farber Cancer Institute, Boston, Massachusetts
    Nature 369:242-5. 1994
    ..Here we describe a two-component system in Saccharomyces cerevisiae that regulates an osmosensing MAP kinase cascade...
  17. pmc Aurora kinases and protein phosphatase 1 mediate chromosome congression through regulation of CENP-E
    Yumi Kim
    Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 142:444-55. 2010
    ....
  18. pmc KNL1/Spc105 recruits PP1 to silence the spindle assembly checkpoint
    Jessica S Rosenberg
    Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065, USA
    Curr Biol 21:942-7. 2011
    ..We propose that the PP1-Spc105 interaction enables local regulation of dynamic phosphorylation and dephosphorylation at the kinetochore to couple microtubule attachment and SAC silencing...
  19. pmc The type 2C phosphatase Wip1: an oncogenic regulator of tumor suppressor and DNA damage response pathways
    Xiongbin Lu
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA
    Cancer Metastasis Rev 27:123-35. 2008
    ..We present evidence that the suppression of p53, p38 MAP kinase, and ATM/ATR signaling pathways by Wip1 are important components of its oncogenicity when it is amplified and overexpressed in human cancers...
  20. pmc Malin decreases glycogen accumulation by promoting the degradation of protein targeting to glycogen (PTG)
    Carolyn A Worby
    Department of Pharmacology, University of California at San Diego, La Jolla, California 92093 0721, USA
    J Biol Chem 283:4069-76. 2008
    ..These results suggest an additional mechanism, involving laforin and malin, in regulating glycogen metabolism...
  21. pmc Protein phosphatase-1 activates CDK9 by dephosphorylating Ser175
    Tatiana Ammosova
    Center for Sickle Cell Disease, Howard University, Washington, DC, United States of America
    PLoS ONE 6:e18985. 2011
    ..Collectively, our results point to CDK9 Ser175 as novel PP1-regulatory site which dephosphorylation upregulates CDK9 activity and contribute to the activation of HIV-1 transcription...
  22. pmc Ypi1, a positive regulator of nuclear protein phosphatase type 1 activity in Saccharomyces cerevisiae
    Jennifer P Bharucha
    Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
    Mol Biol Cell 19:1032-45. 2008
    ..These results, together with genetic interactions among YPI1, GLC7, and SDS22 mutants, indicate that Ypi1 and Sds22 are positive regulators of the nuclear Glc7 activity that is required for mitosis...
  23. ncbi Small CTD phosphatases function in silencing neuronal gene expression
    Michele Yeo
    Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Science 307:596-600. 2005
    ..Likewise, small interfering RNA directed to the single Drosophila SCP unmasks neuronal gene expression in S2 cells. Thus, SCP activity is an evolutionarily conserved transcriptional regulator that acts globally to silence neuronal genes...
  24. pmc PYR/PYL/RCAR family members are major in-vivo ABI1 protein phosphatase 2C-interacting proteins in Arabidopsis
    Noriyuki Nishimura
    Division of Biological Sciences, Cell and Developmental Biology Section and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0116, USA
    Plant J 61:290-9. 2010
    ....
  25. pmc Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors
    John C Meadows
    Division of Biomedical Cell Biology, Warwick Medical School, University of Warwick, Gibbet Hill, Coventry, UK
    Dev Cell 20:739-50. 2011
    ..These data demonstrate that at least two distinct pools of PP1, one kinetochore associated and the other motor associated, are needed to silence the spindle checkpoint...
  26. pmc Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis
    Markus Posch
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Biol 191:61-74. 2010
    ..Sds22 regulates PP1 targeting to the kinetochore, accumulation of phospho-aurora B, and force generation at the kinetochore-microtubule interface...
  27. pmc Autophosphorylation of ataxia-telangiectasia mutated is regulated by protein phosphatase 2A
    Aaron A Goodarzi
    Department of Biological Sciences, University of Calgary, Calgary, AB, Canada
    EMBO J 23:4451-61. 2004
    ..We propose that PP2A plays an important role in the regulation of ATM autophosphorylation and activity in vivo...
  28. ncbi Dephosphorylation of the linker regions of Smad1 and Smad2/3 by small C-terminal domain phosphatases has distinct outcomes for bone morphogenetic protein and transforming growth factor-beta pathways
    Gopal Sapkota
    Cancer Biology and Genetics Program, Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 281:40412-9. 2006
    ....
  29. ncbi Regulation of the antioncogenic Chk2 kinase by the oncogenic Wip1 phosphatase
    H Fujimoto
    1Department of Genome Sciences, Faculty of Medical Sciences, Graduate School of Medicine, Kobe University, 7 5 1, Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    Cell Death Differ 13:1170-80. 2006
    ....
  30. pmc Stress-induced gene expression requires programmed recovery from translational repression
    Isabel Novoa
    Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    EMBO J 22:1180-7. 2003
    ....
  31. doi PPM1D is a potential therapeutic target in ovarian clear cell carcinomas
    David S P Tan
    Department of Histopathology and Gynecologic Oncology, The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, Royal Marsden Hospital, London, United Kingdom
    Clin Cancer Res 15:2269-80. 2009
    ..To identify therapeutic targets in ovarian clear cell carcinomas, a chemoresistant and aggressive type of ovarian cancer...
  32. pmc Quantitative proteomic analysis of purified yeast kinetochores identifies a PP1 regulatory subunit
    Bungo Akiyoshi
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genes Dev 23:2887-99. 2009
    ..These data suggest that Fin1 is a PP1 regulatory subunit whose spatial and temporal activity must be precisely controlled to ensure genomic stability...
  33. pmc PP2B and PP1alpha cooperatively disrupt 7SK snRNP to release P-TEFb for transcription in response to Ca2+ signaling
    Ruichuan Chen
    Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China
    Genes Dev 22:1356-68. 2008
    ..Thus, through cooperatively dephosphorylating Cdk9 in response to Ca2+ signaling, PP2B and PP1alpha alter the P-TEFb functional equilibrium through releasing P-TEFb from 7SK snRNP for transcription...
  34. pmc PHLiPPing the switch on Akt and protein kinase C signaling
    John Brognard
    Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093 0721, USA
    Trends Endocrinol Metab 19:223-30. 2008
    ..This review focuses on the function of PHLPP1 and PHLPP2 in modulating signaling by Akt and PKC...
  35. pmc Spinophilin directs protein phosphatase 1 specificity by blocking substrate binding sites
    Michael J Ragusa
    Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence, Rhode Island, USA
    Nat Struct Mol Biol 17:459-64. 2010
    ..Thus, our work provides the molecular basis for the ability of spinophilin to dictate PP1 substrate specificity...
  36. ncbi The physiological relevance of protein phosphatase 1 and its interacting proteins to health and disease
    M Fardilha
    Centre for Cell Biology, University of Aveiro, Aveiro, Portugal
    Curr Med Chem 17:3996-4017. 2010
    ..Further, it compiles 129 PP1-PIP relevant physiological interactions that are well documented in the literature. Finally, the use of PIPs as therapeutic targets will be addressed...
  37. ncbi Protein phosphatase 1 is a molecular constraint on learning and memory
    David Genoux
    Institute of Cell Biology, Swiss Federal Institute of Technology, Department of Biology, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Nature 418:970-5. 2002
    ..Our findings emphasize the physiological importance of PP1 as a suppressor of learning and memory, and as a potential mediator of cognitive decline during ageing...
  38. pmc Regulation of ATM/p53-dependent suppression of myc-induced lymphomas by Wip1 phosphatase
    Sathyavageeswaran Shreeram
    Institute of Molecular and Cell Biology, Singapore, 138673
    J Exp Med 203:2793-9. 2006
    ..We propose that Wip1 phosphatase is critical for regulating the ATM-mediated tumor surveillance network...
  39. pmc Temperature-sensitive ipl1-2/Aurora B mutation is suppressed by mutations in TOR complex 1 via the Glc7/PP1 phosphatase
    Kelly Tatchell
    Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA
    Proc Natl Acad Sci U S A 108:3994-9. 2011
    ..Together, our results suggest that TORC1 positively regulates the Glc7 activity that opposes Ipl1 and provide a mechanism to tie nutritional status with mitotic regulation...
  40. pmc Medulloblastomas overexpress the p53-inactivating oncogene WIP1/PPM1D
    Robert C Castellino
    Department of Pediatrics, Texas Children s Cancer Center, Baylor College of Medicine, 6621 Fannin Street, MC 3 3320, Houston, TX 77030, USA
    J Neurooncol 86:245-56. 2008
    ..These results indicate that medulloblastoma express significant levels of WIP1 that modulate genotoxic responsiveness by negatively regulating p53...
  41. ncbi The PP2A-associated protein alpha4 is an essential inhibitor of apoptosis
    Mei Kong
    Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 306:695-8. 2004
    ..Thus, mammalian cell viability depends on repression of transcription-initiated apoptosis mediated by a component of PP2A...
  42. pmc AMP-activated protein kinase phosphorylates R5/PTG, the glycogen targeting subunit of the R5/PTG-protein phosphatase 1 holoenzyme, and accelerates its down-regulation by the laforin-malin complex
    Santiago Vernia
    Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Científicas CSIC and CIBER de Enfermedades Raras CIBERER, Jaime Roig 11, Valencia 46010, Spain
    J Biol Chem 284:8247-55. 2009
    ..Thus, our results define a novel role of AMPK in glycogen homeostasis...
  43. pmc Microarray expression analyses of Arabidopsis guard cells and isolation of a recessive abscisic acid hypersensitive protein phosphatase 2C mutant
    Nathalie Leonhardt
    Cell and Developmental Biology Section, Division of Biological Sciences, and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0116, USA
    Plant Cell 16:596-615. 2004
    ..A T-DNA disruption mutation in AtP2C-HA confers ABA-hypersensitive regulation of stomatal closing and seed germination. The presented data provide a basis for cell type-specific genomic scale analyses of gene function...
  44. ncbi Downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit in budding yeast
    Ethel Queralt
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Cell 125:719-32. 2006
    ..These findings allow us to present a new quantitative model for mitotic exit in budding yeast...
  45. pmc PTG gene deletion causes impaired glycogen synthesis and developmental insulin resistance
    Sean M Crosson
    Department of Medicine, Life Sciences Institute, University of Michigan School of Medicine, Ann Arbor, Michigan 48109 0650, USA
    J Clin Invest 111:1423-32. 2003
    ..These data suggest that PTG plays a critical role in glycogen synthesis and is necessary to maintain the appropriate metabolic balance for the partitioning of fuel substrates between glycogen and lipid...
  46. ncbi The tumor suppressor PP2A is functionally inactivated in blast crisis CML through the inhibitory activity of the BCR/ABL-regulated SET protein
    Paolo Neviani
    Human Cancer Genetics Program, Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University, Columbus, Ohio 43210, USA
    Cancer Cell 8:355-68. 2005
    ..Thus, functional inactivation of PP2A is essential for BCR/ABL leukemogenesis and, perhaps, required for blastic transformation...
  47. doi Modulation of drought resistance by the abscisic acid receptor PYL5 through inhibition of clade A PP2Cs
    Julia Santiago
    Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas UPV, ES 46022 Valencia, Spain
    Plant J 60:575-88. 2009
    ..Moreover, we show that enhanced resistance to drought can be obtained through PYL5-mediated inhibition of clade A PP2Cs...
  48. doi Evolution of protein phosphatases in plants and animals
    Greg B G Moorhead
    Department of Biological Sciences, University of Calgary, 2500 University Drive N W, Calgary, Alberta, Canada
    Biochem J 417:401-9. 2009
    ..In the present review, we discuss the evolution of the PTPs, the serine/threonine specific PPP (phosphoprotein phosphatase) and PPM (metallo-dependent protein phosphatase) families and the more recently discovered phosphatases ..
  49. pmc Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling
    V Janssens
    Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Biochem J 353:417-39. 2001
    ..Finally, the de-regulation of PP2A in some specific pathologies will be touched upon...
  50. ncbi A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana
    K Meyer
    Institute of Plant Sciences, Swiss Federal Institute of Technology, Zurich
    Science 264:1452-5. 1994
    ..Thus, the control of the phosphorylation state of cell signaling components by the ABI1 product could mediate pleiotropic hormone responses...
  51. ncbi Germline mutations in PTEN are present in Bannayan-Zonana syndrome
    D J Marsh
    Nat Genet 16:333-4. 1997
  52. pmc Bistability in the Ca(2+)/calmodulin-dependent protein kinase-phosphatase system
    A M Zhabotinsky
    Department of Chemistry and Volen Center for Complex Systems, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Biophys J 79:2211-21. 2000
    ..They also suggest a plausible explanation for the very high concentrations of CaMKII found in postsynaptic densities of cerebral neurons...
  53. ncbi Protein phosphatase 2A: a panoply of enzymes
    D M Virshup
    Departments of Pediatrics and Oncological Sciences, 5C334 School of Medicine, University of Utah, Salt Lake City, 84132, USA david
    Curr Opin Cell Biol 12:180-5. 2000
    ..During the past year, studies have shown that protein phosphatase 2A influences events ranging from the initiation of DNA replication to vertebrate axis formation to apoptosis...
  54. pmc PP1-mediated dephosphorylation of phosphoproteins at mitotic exit is controlled by inhibitor-1 and PP1 phosphorylation
    Judy Qiju Wu
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Nat Cell Biol 11:644-51. 2009
    ..Thus, Cdc2 both phosphorylates multiple mitotic substrates and inhibits their PP1-mediated dephosphorylation...
  55. pmc PP4 and PP2A regulate Hedgehog signaling by controlling Smo and Ci phosphorylation
    Hongge Jia
    Sealy Center for Cancer Cell Biology, Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USA
    Development 136:307-16. 2009
    ..Taken together, our results suggest that PP4 and PP2A are two phosphatases that act at different positions of the Hh signaling cascade...
  56. ncbi The myeloid leukemia-associated protein SET is a potent inhibitor of protein phosphatase 2A
    M Li
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Milton S Hershey Medical Center, Hershey 17033, USA
    J Biol Chem 271:11059-62. 1996
    ..The results indicate that SET is a potent and specific inhibitor of PP2A and suggest that impaired regulation of PP2A may contribute to acute myeloid leukemogenesis...
  57. pmc Protein phosphatase 1 regulates exit from the spindle checkpoint in budding yeast
    Benjamin A Pinsky
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, PO Box 19024, Seattle, WA 98109, USA
    Curr Biol 19:1182-7. 2009
    ..We therefore propose that PP1 activity silences the checkpoint by reversing key phosphorylation events...
  58. pmc A novel and essential mechanism determining specificity and activity of protein phosphatase 2A (PP2A) in vivo
    Thomas Fellner
    Institute of Medical Biochemistry, Division of Molecular Biology, Vienna Biocenter, University of Vienna, A 1030 Vienna, Austria
    Genes Dev 17:2138-50. 2003
    ..Complementation and RNA-interference experiments showed that PTPA fulfills an essential function conserved from yeast to man...
  59. pmc Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1
    J Warmka
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Mol Cell Biol 21:51-60. 2001
    ..Consistent with its role as a negative regulator of Hog1, which accumulates in the nucleus upon activation, Ptc1 was found in both the nucleus and the cytoplasm. Thus, one function of Ptc1 is to inactivate Hog1...
  60. pmc Glucocorticoids inhibit MAP kinase via increased expression and decreased degradation of MKP-1
    O Kassel
    Forschungszentrum Karlsruhe, Institute of Toxicology and Genetics, PO Box 3640, D-76021 Karlsruhe, Germany
    EMBO J 20:7108-16. 2001
    ..These results identify MKP-1 as essential for glucocorticoid-mediated control of Erk-1/2 activation and unravel a novel regulatory mechanism for this anti-inflammatory drug...
  61. ncbi Zebrafish pten genes have overlapping and non-redundant functions in tumorigenesis and embryonic development
    A Faucherre
    Hubrecht Institute, Utrecht, The Netherlands
    Oncogene 27:1079-86. 2008
    ..These pten mutant zebrafish represent a unique model to screen for genetic and/or chemical suppressors of Pten loss-of-function...
  62. ncbi Identification of multicopy suppressors of cell cycle arrest at the G1-S transition in Saccharomyces cerevisiae
    Ivan Munoz
    Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona
    Yeast 20:157-69. 2003
    ..The possible relationship between the known functions of these suppressor genes and the progress through the G(1)-S transition is discussed...
  63. doi Spindle checkpoint silencing: PP1 tips the balance
    Bart Lesage
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, University of Leuven, B 3000 Leuven, Belgium
    Curr Biol 21:R898-903. 2011
    ..In addition, PP1 also directly inactivates a kinetochore-associated pool of Aurora B and silences checkpoint signaling by opposing the centromeric targeting of Aurora B...
  64. doi Isoform-specific roles of protein phosphatase 1 catalytic subunits in sarcoplasmic reticulum-mediated Ca(2+) cycling
    Hidekazu Aoyama
    Division of Cardiology, Department of Medicine and Clinical Science, Yamaguchi University Graduate School of Medicine, 1 1 1 Minami kogushi, Ube, Yamaguchi 755 8505, Japan
    Cardiovasc Res 89:79-88. 2011
    ..We sought to determine the functional contribution of each PP1 isoform to sarcoplasmic reticulum (SR)-mediated Ca(2+) cycling in isolated adult rat cardiomyocytes...
  65. pmc Structural and functional insights into core ABA signaling
    Joshua J Weiner
    Department of Biochemistry and Center for Eukaryotic Structural Genomics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Curr Opin Plant Biol 13:495-502. 2010
    ....
  66. pmc A third metal is required for catalytic activity of the signal-transducing protein phosphatase M tPphA
    Jiyong Su
    Interfaculty Institute of Microbiology and Infection Medicine, Department of Organismic Interactions, University of Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    J Biol Chem 286:13481-8. 2011
    ..Mutation of the homologue Asp residue in human PP2Cα also caused loss of function, suggesting a general requirement of M3 in PPM-catalyzed reactions...
  67. pmc mTOR-dependent regulation of PHLPP expression controls the rapamycin sensitivity in cancer cells
    Jianyu Liu
    Markey Cancer Center, University of Kentucky, Lexington, Kentucky 40536 0509, USA
    J Biol Chem 286:6510-20. 2011
    ....
  68. doi The oncogenic phosphatase PPM1D confers cisplatin resistance in ovarian carcinoma cells by attenuating checkpoint kinase 1 and p53 activation
    A Y Ali
    Departments of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada
    Oncogene 31:2175-86. 2012
    ..These findings extend the current knowledge on the molecular and cellular basis of cisplatin resistance and offer the rationale for PPMID as a potential target for treatment of chemoresistant OVCA...
  69. pmc Protein phosphatase 1 (PP1) is a post-translational regulator of the mammalian circadian clock
    Isabelle Schmutz
    Department of Biology, Unit of Biochemistry, University of Fribourg, Fribourg, Switzerland
    PLoS ONE 6:e21325. 2011
    ..Hence, PP1, may reduce PER2 phosphorylation thereby influencing nuclear localization of this protein. This may at least partially influence period and phase shifting properties of the mammalian circadian clock...
  70. ncbi Degeneracy and function of the ubiquitous RVXF motif that mediates binding to protein phosphatase-1
    Paulina Wakula
    Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Biol Chem 278:18817-23. 2003
    ....
  71. pmc Role of host protein tyrosine phosphatase SHP-1 in Leishmania donovani-induced inhibition of nitric oxide production
    Geneviève Forget
    Centre de Recherche en Infectiologie, Centre Hospitalier Universitaire de Quebec, Canada
    Infect Immun 74:6272-9. 2006
    ....
  72. doi Protein phosphatase-1 regulates Akt1 signal transduction pathway to control gene expression, cell survival and differentiation
    L Xiao
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, 68198 5870, USA
    Cell Death Differ 17:1448-62. 2010
    ..These results show that PP-1 acts as a major phosphatase to dephosphorylate AKT at Thr-450 and thus modulate its functions...
  73. pmc Protein phosphatases and their regulation in the control of mitosis
    Satoru Mochida
    Kumamoto University, Honjo, Kumamoto City, Kumamoto, Japan
    EMBO Rep 13:197-203. 2012
    ..Here, we summarize our present understanding of the phosphatases that are important in the control of the cell cycle and pose the questions that need to be answered as regards the regulation of protein phosphatases...
  74. ncbi Structural and binding studies of the three-metal center in two mycobacterial PPM Ser/Thr protein phosphatases
    Annemarie Wehenkel
    Unité de Biochimie Structurale and CNRS URA 2185, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Mol Biol 374:890-8. 2007
    ....
  75. pmc Loss of PHLPP expression in colon cancer: role in proliferation and tumorigenesis
    J Liu
    Department of Biochemistry and Molecular Biology, West China Medical School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, PR China
    Oncogene 28:994-1004. 2009
    ..Moreover, reexpression of either PHLPP isoform in HCT116 cells inhibited tumor growth in vivo. Taken together, our results strongly support a tumor suppressor role of PHLPP in colon cancer...
  76. pmc Phosphorylation of Chk1 by ATR is antagonized by a Chk1-regulated protein phosphatase 2A circuit
    Van Leung-Pineda
    Department of Cell Biology and Physiology and Howard Hughes Medical Institute, Washington University School of Medicine, Box 8228, 660 South Euclid Ave, St Louis, MO 63110, USA
    Mol Cell Biol 26:7529-38. 2006
    ....
  77. pmc Inhibition of protein kinase R activation and upregulation of GADD34 expression play a synergistic role in facilitating coronavirus replication by maintaining de novo protein synthesis in virus-infected cells
    Xiaoxing Wang
    Institute of Molecular and Cell Biology, Proteos, Singapore
    J Virol 83:12462-72. 2009
    ..e., blocking PKR activation and inducing GADD34 expression, to maintain de novo protein synthesis in IBV-infected cells and, meanwhile, to enhance viral replication...
  78. ncbi Multiple interactions within the AKAP220 signaling complex contribute to protein phosphatase 1 regulation
    R V Schillace
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health Sciences University, Portland, Oregon 97201 3098, USA
    J Biol Chem 276:12128-34. 2001
    ..These experiments indicate that regulation of PP1 activity by AKAP220 involves a complex network of intra- and intermolecular interactions...
  79. ncbi Activation of protein phosphatase 2A by palmitate inhibits AMP-activated protein kinase
    Yong Wu
    Division of Endocrinology and Diabetes, Department of Medicine, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 282:9777-88. 2007
    ..Taken together, we conclude that palmitate inhibits the phosphorylation of both AMPK and endothelial nitric-oxide synthase in endothelial cells via ceramide-dependent PP2A activation...
  80. ncbi The scaffolding A/Tpd3 subunit and high phosphatase activity are dispensable for Cdc55 function in the Saccharomyces cerevisiae spindle checkpoint and in cytokinesis
    Roni Koren
    Gonda Center of Molecular Microbiology and The Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine, Technion Israel Institute of Technology, Bat Galim, Haifa, 31096, Israel
    J Biol Chem 279:48598-606. 2004
    ..This association requires C subunit carboxyl methylation. The residual phosphatase activity associated with Cdc55 in the absence of Tpd3 is sufficient to maintain a partially active spindle checkpoint and to prevent cytokinesis defects...
  81. ncbi A selective inhibitor of eIF2alpha dephosphorylation protects cells from ER stress
    Michael Boyce
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Science 307:935-9. 2005
    ..More broadly, salubrinal demonstrates the feasibility of selective pharmacological targeting of cellular dephosphorylation events...
  82. pmc Expression of a protein phosphatase 1 inhibitor, cdNIPP1, increases CDK9 threonine 186 phosphorylation and inhibits HIV-1 transcription
    Tatiana Ammosova
    Center for Sickle Cell Disease, Howard University, Washington, DC 20001, USA
    J Biol Chem 286:3798-804. 2011
    ..Expression of cdNIPP1 as a part of the HIV-1 genome inhibited HIV-1 replication. Our study provides a proof-of-concept for the future development of PP1-targeting compounds as inhibitors of HIV-1 replication...
  83. ncbi MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo
    H Sun
    Cold Spring Harbor Laboratory, New York 11724 2208
    Cell 75:487-93. 1993
    ..The mutant 3CH134 protein also forms a physical complex with the phosphorylated form of p42MAPK. These findings suggest that 3CH134 is a physiological MAP kinase phosphatase; we propose the name MKP-1 for this phosphatase...
  84. pmc Sds22/PP1 links epithelial integrity and tumor suppression via regulation of myosin II and JNK signaling
    Y Jiang
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Oncogene 30:3248-60. 2011
    ....
  85. ncbi Differential regulation of MAP kinase signalling by dual-specificity protein phosphatases
    D M Owens
    Cancer Research UK Stress Response Laboratory, Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee, Scotland, UK
    Oncogene 26:3203-13. 2007
    ....
  86. pmc Displacement affinity chromatography of protein phosphatase one (PP1) complexes
    Greg B G Moorhead
    Department of Biological Sciences, University of Calgary, 2500 University Dr, NW Calgary, AB T2N 1N4, Canada
    BMC Biochem 9:28. 2008
    ..PP1 is localized to its site of action by interacting with targeting or regulatory proteins, a majority of which contains a primary docking site referred to as the RVXF/W motif...
  87. pmc Dynamic regulation of pro- and anti-inflammatory cytokines by MAPK phosphatase 1 (MKP-1) in innate immune responses
    Hongbo Chi
    Section of Immunobiology, Department of Pharmacology, and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 103:2274-9. 2006
    ..These results highlight the complex mechanisms by which the MAPKs regulate innate immunity...
  88. pmc Dephosphorylation of CDK9 by protein phosphatase 2A and protein phosphatase-1 in Tat-activated HIV-1 transcription
    Tatyana Ammosova
    Center for Sickle Cell Disease, Howard University, 2121 Georgia Ave, N W Washington, DC 20059, USA
    Retrovirology 2:47. 2005
    ..In the present study we analyze relative contribution of PP2A and PP1 to dephosphorylation of CDK9 and to HIV-1 transcription in vitro and in vivo...
  89. doi Schizosaccharomyces pombe cell division cycle under limited glucose requires Ssp1 kinase, the putative CaMKK, and Sds23, a PP2A-related phosphatase inhibitor
    Yuichiro Hanyu
    CREST Research Project, Japan Science Technology Corporation, Kyoto, Japan
    Genes Cells 14:539-54. 2009
    ..Ssp1 and Ppe1 thus seem to antagonize in utilizing limited glucose. We also show that Ppk9 and Ssp2 are the catalytic subunits of AMPK and AMPK-related kinases, respectively, which bind to common beta-(Amk2) and gamma-(Cbs2) subunits...
  90. ncbi MAPK phosphatases--regulating the immune response
    Yusen Liu
    Center for Perinatal Research, Columbus Children s Research Institute, Columbus Children s Hospital, Department of Pediatrics, The Ohio State University College of Medicine, Columbus, Ohio 43205, USA
    Nat Rev Immunol 7:202-12. 2007
    ..This Review discusses the properties, function and regulation of MKPs during immune responses...
  91. pmc Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis
    Santiago Mora-Garcia
    Plant Biology Labo ato y, The Salk Institute fo Biological Studies, and the Howa d Hughes Medical Institute, La Jolla, Califo nia 92037, USA
    Genes Dev 18:448-60. 2004
    ..Our data indicate that BES1 is subject to antagonistic phosphorylation and dephosphorylation reactions in the nucleus, which fine-tune the amplitude of the response to BL...
  92. pmc Protein phosphatases decrease sarcoplasmic reticulum calcium content by stimulating calcium release in cardiac myocytes
    Dmitry Terentyev
    Texas Tech University Health Sciences Center, 3601 4th Street, Lubbock, TX 79430 6551, USA
    J Physiol 552:109-18. 2003
    ..Our results suggest that increased intracellular phosphatase activity stimulates RyR-mediated SR Ca2+ release leading to depleted SR Ca2+ stores in cardiac myocytes...
  93. pmc Generation of active protein phosphatase 2A is coupled to holoenzyme assembly
    Hans Hombauer
    Department of Medical Biochemistry, Max F Perutz Laboratories, Medical University of Vienna, Vienna, Austria
    PLoS Biol 5:e155. 2007
    ..We propose a novel model of PP2A biogenesis in which a tightly controlled activation cascade protects cells from untargeted activity of the free catalytic PP2A subunit...
  94. ncbi Protein phosphatases take the mitotic stage
    Peter De Wulf
    European Institute of Oncology, Department of Experimental Oncology, IFOM IEO Campus, Milan, Italy
    Curr Opin Cell Biol 21:806-15. 2009
    ..Recent work has now exposed the conserved serine-threonine phosphatases PP1 and PP2A as key regulators of various mitotic processes...
  95. ncbi PPP1R6, a novel member of the family of glycogen-targetting subunits of protein phosphatase 1
    C G Armstrong
    Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 418:210-4. 1997
    ..The PPP1R6 is associated with glycogen in muscle but is not subject to the same modes of covalent and allosteric regulation as G(M) and G(L)...
  96. doi Genetic control of cellular quiescence in S. pombe
    Kenichi Sajiki
    Okinawa Institute of Science and Technology OIST, Initial Research Project, Uruma 904 2234, Okinawa, Japan
    J Cell Sci 122:1418-29. 2009
    ..We propose that the transcriptional role of Fcp1 is central in differentiating quiescence from proliferation...
  97. ncbi The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm
    B McCright
    Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City, 84112, USA
    J Biol Chem 271:22081-9. 1996
    ..These studies demonstrate an increasing diversity of regulatory mechanisms to control the activity of this key intracellular protein phosphatase and suggest distinct functions for isoforms targeted to different intracellular locations...
  98. ncbi Regulation of the TAK1 signaling pathway by protein phosphatase 2C
    M Hanada
    Department of Biochemistry, Institute of Development, Aging, and Cancer, Tohoku University, 4 1 Seiryomachi, Aoba ku, Sendai 980 8575, Japan
    J Biol Chem 276:5753-9. 2001
    ..Collectively, these results indicate that PP2Cbeta negatively regulates the TAK1 signaling pathway by direct dephosphorylation of TAK1...
  99. ncbi Protein phosphatase 2A: variety of forms and diversity of functions
    K Lechward
    Cell and Molecular Signaling Laboratory, , Poland
    Acta Biochim Pol 48:921-33. 2001
    ..Studies on PP2A have shown that this enzyme may be implicated in the regulation of metabolism, transcription, RNA splicing, translation, differentiation, cell cycle, oncogenic transformation and signal transduction...
  100. ncbi TIP41 interacts with TAP42 and negatively regulates the TOR signaling pathway
    E Jacinto
    Division of Biochemistry, Biozentrum, University of Basel, CH 4056, Basel, Switzerland
    Mol Cell 8:1017-26. 2001
    ....
  101. ncbi Negative regulation of mitosis by the fission yeast protein phosphatase ppa2
    N Kinoshita
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    Genes Dev 7:1059-71. 1993
    ....

Research Grants81

  1. REGULATORY DOMAINS OF PROTEIN PHOSPHATASE 1
    PETER KENNELLY; Fiscal Year: 1999
    ..Successful completion of this project may increase the understanding of how PP1 is regulated in cells, and do so in molecular detail. ..
  2. STRUCTURE, BIOSYNTHESIS AND FUNCTION OF GLYCOPROTEINS
    Stuart Kornfeld; Fiscal Year: 2007
    ..If it is novel, we will clone it to obtain its sequence and identify protein domains that may give clues as to its function. We will then study how the protein facilitates AP-1 recruitment.[unreadable]..
  3. CERAMIDE IN HUMAN NEUROBLASTOMA DIFFERENTIATION /THERAPY
    JAQUELINE KRAVEKA; Fiscal Year: 2002
    ....
  4. The Effects of PP2A on TNF Signaling and Smoke-Induced Lung Injury
    Robert F Foronjy; Fiscal Year: 2010
    ..This proposal will advance public health by determining how PP2A, the primary eukaryotic serine/threonine phosphatase, alters the injurious responses to cigarette smoke exposure in the lung. ..
  5. REGION SPECIFIC PHOSPHOPROTEINS IN THE BASAL GANGLIA
    Paul Greengard; Fiscal Year: 1999
    ..Scientific Core) will include the production and supply of key reagents for the other sections of this Program Project. ..
  6. Regulation of Drosophila arrestins in light adaptation
    Bih Hwa Shieh; Fiscal Year: 2010
    ..We propose that CaMKII, by promoting phosphorylation of visual arrestins, is critical for the negative regulation of the visual response. Moreover, the activity of CaMKII may be tightly modulated by PP2A. ..
  7. Regulation of Drosophila arrestins in light adaptation
    Bih Hwa Shieh; Fiscal Year: 2009
    ..We propose that CaMKII, by promoting phosphorylation of visual arrestins, is critical for the negative regulation of the visual response. Moreover, the activity of CaMKII may be tightly modulated by PP2A. ..
  8. CFTR REGULATION BY TARGETED KINASE AND PHOSPHATASE
    Neil Bradbury; Fiscal Year: 2000
    ..abstract_text> ..
  9. CFTR REGULATION BY TARGETED KINASE AND PHOSPHATASE
    Neil Bradbury; Fiscal Year: 2001
    ..abstract_text> ..
  10. CFTR REGULATION BY TARGETED KINASE AND PHOSPHATASE
    Neil Bradbury; Fiscal Year: 2001
    ..abstract_text> ..
  11. CFTR REGULATION BY TARGETED KINASE AND PHOSPHATASE
    Neil Bradbury; Fiscal Year: 2002
    ..abstract_text> ..
  12. CFTR REGULATION BY TARGETED KINASE AND PHOSPHATASE
    Neil Bradbury; Fiscal Year: 1999
    ..abstract_text> ..
  13. Biosynthesis of Microbial Polyketides
    David E Cane; Fiscal Year: 2010
    ..Finally, the proposed studies will lead to fundamental new biological insights into the central issue of modern molecular biochemistry, the relationship between protein sequence, structure, and function. ..
  14. Cardiac Sarcoplasmic Reticulum Calcium Cycling Proteins
    Evangelia G Kranias; Fiscal Year: 2010
    ..Our studies will clearly advance our knowledge on these two protein players involved in cardiac function and dysfunction, which may lead to better therapeutic avenues in heart failure. ..
  15. REGULATION OF HEPATIC GLUTAHIONE SYNTHESIS
    Shelly Lu; Fiscal Year: 1992
    ..The ultimate goal of this work is to utilize this information to impact on the treatment and prevention of various complications that may result from altered hepatic GSH synthesis...
  16. FLUORESCENCE STUDIES OF ENZYME STRUCTURE AND FUNCTION
    Sonia Anderson; Fiscal Year: 1992
    ..Calmodulin, cAMP-dependent protein kinase, and phosphoprotein phosphatase PrP-1 play roles in both the PFK and MLCK systems...
  17. Novel Screen for Targeted CLL Therapeutics
    Dale Christensen; Fiscal Year: 2009
    ..We now propose to develop a peptide displacement assay that can be used to screen compound libraries to identify small molecule SET antagonists and screen a diverse 30,000 compound library. ..
  18. Combined neuroprotection and metabolic correction to treat leukodystrophies
    Ernesto Bongarzone; Fiscal Year: 2009
    ....
  19. Combined neuroprotection and metabolic correction to treat leukodystrophies
    ERNESTO ROQUE BONGARZONE; Fiscal Year: 2010
    ....
  20. Protein Phosphatase Inhibitor-1 and vascular smooth muscle cell function
    Lahouaria Hadri; Fiscal Year: 2010
    ..Our goal is to focus on strategies that inhibit VSMC proliferation and migration and enhance re-endothelization by using somatic gene therapy carrying I-1 to provide a treatment that prevents restonosis. (End of Abstract) ..
  21. PROTEIN PHOSPHATASES AND OOCYTE MEIOSIS
    Gary Smith; Fiscal Year: 2001
    ..These studies will provide novel information concerning control of oocyte maturation and spindle formation that may subsequently influence the developmental competency of resulting embryos. ..
  22. C-TRIP: Targeted Gene Therapy for the Treatment of Heart Failure (P20)
    ROGER JOSEPH HAJJAR; Fiscal Year: 2010
    ..This new cardiotropic vector combined with a novel well validated target offers a new strategy for the treatment of heart failure. ..
  23. PROTEIN PHOSPHATASES AND OOCYTE MEIOSIS
    Gary Smith; Fiscal Year: 1999
    ..These studies will provide novel information concerning control of oocyte maturation and spindle formation that may subsequently influence the developmental competency of resulting embryos. ..
  24. PROTEIN PHOSPHATASES AND OOCYTE MEIOSIS
    Gary Smith; Fiscal Year: 2000
    ..These studies will provide novel information concerning control of oocyte maturation and spindle formation that may subsequently influence the developmental competency of resulting embryos. ..
  25. PROTEIN PHOSPHATASES AND OOCYTE MEIOSIS
    Gary Smith; Fiscal Year: 2002
    ..These studies will provide novel information concerning control of oocyte maturation and spindle formation that may subsequently influence the developmental competency of resulting embryos. ..
  26. Myocardial Ischemia-Reperfusion and Protein Phosphatase 2a
    Polly A Hofmann; Fiscal Year: 2010
    ..the heart, can we improve the short and long term outcomes from ischemic events in the heart? Which of the unique PP2aB targeting subunits is key to improving post-ischemic recovery in the heart, and by what mechanisms does this occur? ..
  27. Myocardial Ischemia-Reperfusion and Protein Phosphatase 2a
    Polly Hofmann; Fiscal Year: 2009
    ..the heart, can we improve the short and long term outcomes from ischemic events in the heart? Which of the unique PP2aB targeting subunits is key to improving post-ischemic recovery in the heart, and by what mechanisms does this occur? ..
  28. Mitogen-activated Protein Kinases and NF-kB Activation in Vascular Smooth Muscle
    Bingbing Jiang; Fiscal Year: 2009
    ....
  29. ROLE OF PP2A IN POLYOMAVIRUS TUMOR ANTIGEN FUNCTIONS
    DAVID PALLAS; Fiscal Year: 1992
    ..We will test the abilities of our various proteins mutated in the interaction sites to modulate cellular function and phosphorylation events.es..
  30. Mitogen-activated Protein Kinases and NF-kB Activation in Vascular Smooth Muscle
    Bingbing Jiang; Fiscal Year: 2010
    ....
  31. Induction and maintenance of mitosis by Cdk1 suppression of Protein phosphatase 1
    ROBERT LEWIS MARGOLIS; Fiscal Year: 2010
    ..If our hypothesis is true, our discovery will profoundly change understanding of mitotic control, and thus reveal new targets for cancer chemotherapy. ..
  32. Induction and maintenance of mitosis by Cdk1 suppression of Protein phosphatase 1
    Robert Margolis; Fiscal Year: 2009
    ..If our hypothesis is true, our discovery will profoundly change understanding of mitotic control, and thus reveal new targets for cancer chemotherapy. ..
  33. IN VITRO MODEL OF PRECONDITIONING WITH ISOLATED MYOCYTES
    CHARLES GANOTE; Fiscal Year: 1999
    ....
  34. IN VITRO MODEL OF PRECONDITIONING WITH ISOLATED MYOCYTES
    CHARLES GANOTE; Fiscal Year: 2001
    ....
  35. IN VITRO MODEL OF PRECONDITIONING WITH ISOLATED MYOCYTES
    CHARLES GANOTE; Fiscal Year: 2000
    ....
  36. Phosphorylation & Function of Inhibitor-2
    David L Brautigan; Fiscal Year: 2010
    ..The goal is to understand how this protein acts to connect multiple signaling inputs into centrosome actions. Understanding cellular mechanisms is the basis for diagnosis and treatment of human diseases. ..
  37. Phosphorylation & Function of Inhibitor-2
    DAVID BRAUTIGAN; Fiscal Year: 2009
    ..The goal is to understand how this protein acts to connect multiple signaling inputs into centrosome actions. Understanding cellular mechanisms is the basis for diagnosis and treatment of human diseases. ..
  38. PATHOBIOLOGY OF TAU PROTEIN
    KENNETH KOSIK; Fiscal Year: 1999
    ..Saporin is toxic to cells only when it reaches the cytosol. ..
  39. Small Molecule Screen for Apolipoprotein-E/Alzheimer's Disease
    MICHAEL PETER VITEK; Fiscal Year: 2010
    ..This will require several levels of screening to find the few compounds that can be orally administered and show protective activity in the brains of experimental animals. ..
  40. Role of Protein Phosphatase-1 in Cerebral Ischemia and Cell Death
    Hugh C Hemmings; Fiscal Year: 2010
    ..This approach is targeted to the development of rational mechanism-based protein phosphatase-1 inhibitors to attenuate ischemic cell death and neurological injury. ..
  41. Role of Protein Phosphatase-1 in Cerebral Ischemia and Cell Death
    HUGH HEMMINGS; Fiscal Year: 2009
    ..This approach is targeted to the development of rational mechanism-based protein phosphatase-1 inhibitors to attenuate ischemic cell death and neurological injury. ..
  42. Mechanisms Regulating Reversal of Malignancy
    Priscilla Furth; Fiscal Year: 2006
    ..2. c. Test if pharmacological RXRalpha and/or PPARgamma agonists alone or in combination prevent development of ERalpha ductal hyperplasia and DCIS. ..
  43. Caveolin 1: a novel modulator of the PP2A/ATM/p53 pathway
    Ferruccio Galbiati; Fiscal Year: 2009
    ....
  44. Mechanisms Regulating Reversal of Malignancy
    Priscilla Furth; Fiscal Year: 2007
    ..2. c. Test if pharmacological RXRalpha and/or PPARgamma agonists alone or in combination prevent development of ERalpha ductal hyperplasia and DCIS. ..
  45. Mechanisms Regulating Reversal of Malignancy
    Priscilla Furth; Fiscal Year: 2009
    ..2. c. Test if pharmacological RXRalpha and/or PPARgamma agonists alone or in combination prevent development of ERalpha ductal hyperplasia and DCIS. ..
  46. Caveolin 1: a novel modulator of the PP2A/ATM/p53 pathway
    Ferruccio Galbiati; Fiscal Year: 2010
    ....
  47. Role of Protein Phosphatase-1 in Cerebral Ischemia and Cell Death
    HUGH HEMMINGS; Fiscal Year: 2009
    ..This approach is targeted to the development of rational mechanism-based protein phosphatase-1 inhibitors to attenuate ischemic cell death and neurological injury. ..
  48. Mechanisms Regulating Reversal of Malignancy
    Priscilla Furth; Fiscal Year: 2009
    ..2. c. Test if pharmacological RXRalpha and/or PPARgamma agonists alone or in combination prevent development of ERalpha ductal hyperplasia and DCIS. ..
  49. Phosphorylation & Function of Inhibitor-2
    DAVID BRAUTIGAN; Fiscal Year: 2009
    ..The goal is to understand how this protein acts to connect multiple signaling inputs into centrosome actions. Understanding cellular mechanisms is the basis for diagnosis and treatment of human diseases. ..
  50. REGION-SPECIFIC PHOSHPROTEINS IN THE BASAL GANGLIA
    Paul Greengard; Fiscal Year: 1990
    ....
  51. Antiapoptotic Mechanism of Protein Phosphatase-1 in Lens
    David Li; Fiscal Year: 2005
    ....
  52. SPHINGOLIPIDS & REGULATION OF GROWTH & APOPTOTIC SIGNALI
    DAVID PALLAS; Fiscal Year: 2000
    ..abstract_text> ..
  53. Antiapoptotic Mechanism of Protein Phosphatase-1 in Lens
    David Li; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  54. Antiapoptotic Mechanism of Protein Phosphatase-1 in Lens
    David Li; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  55. MODULATION OF CARDIAC CALCIUM CHANNELS IN VITRO
    Robert Rosenberg; Fiscal Year: 1993
    ..In addition, Ca channels will be solubilized from the sarcolemma and reconstituted into artificial membranes, providing even better control of the channel's regulatory environment...
  56. Differential Regulation of Na-K-Cl Cotransport
    CAROLE LIEDTKE; Fiscal Year: 2009
    ..More importantly, the studies will lay the foundation for predicting and testing the effects of drugs, both pharmaceutical and recreational, that alter NKCC1 function. ..
  57. Differential Regulation of Na-K-Cl Cotransport
    Carole M Liedtke; Fiscal Year: 2010
    ..More importantly, the studies will lay the foundation for predicting and testing the effects of drugs, both pharmaceutical and recreational, that alter NKCC1 function. ..
  58. CARDIAC PHOSPHOPROTEIN PHOSPHATASES
    HENG CHUN LI; Fiscal Year: 1990
    ..The long-term goal of this project is to identify phosphoprotein phosphatase (ptase) isozymes of cardiac muscle and other tissues, and to gain greater understanding of their ..
  59. GDP/GTP Exchange Factors: Nucleus Accumbens Plasticity
    ELIZABETH EIPPER; Fiscal Year: 2004
    ..PP-1, a DARPP-32 target localized to spines, also binds to Kalirin. It is our hypothesis that Kalirin-7, through its interactions with these regulators, integrates the effects of diverse signaling pathways on spine formation. ..
  60. GDP/GTP Exchange Factors: Nucleus Accumbens Plasticity
    ELIZABETH EIPPER; Fiscal Year: 2005
    ..PP-1, a DARPP-32 target localized to spines, also binds to Kalirin. It is our hypothesis that Kalirin-7, through its interactions with these regulators, integrates the effects of diverse signaling pathways on spine formation. ..
  61. GDP/GTP Exchange Factors: Nucleus Accumbens Plasticity
    ELIZABETH EIPPER; Fiscal Year: 2007
    ..It is our hypothesis that Kalirin-7, through its interactions with these regulators, integrates the effects of diverse signaling pathways on spine formation. [unreadable] [unreadable]..
  62. GDP/GTP Exchange Factors: Nucleus Accumbens Plasticity
    ELIZABETH EIPPER; Fiscal Year: 2006
    ..It is our hypothesis that Kalirin-7, through its interactions with these regulators, integrates the effects of diverse signaling pathways on spine formation. [unreadable] [unreadable]..
  63. Dopamine Signaling Mechanisms of Traumatic Brain Injury
    C Dixon; Fiscal Year: 2009
    ..In survivors, TBI results in the persistent disturbance of cognitive functioning. This project seeks to examine key neurochemical mechanisms underlying cognitive deficits and to evaluate novel therapies to maximize recovery of function. ..
  64. REGULATION OF CREB/ATF FUNCTION BY PROTEIN PHOSPHATASES
    Dwight Klemm; Fiscal Year: 1993
    ..The findings should also establish small-t as an important reagent for studying the activity of PP2A in transcriptional regulation...
  65. Reversible phosphorylation in Hedgehog signaling
    ALAN ZHU; Fiscal Year: 2010
    ....
  66. Reversible phosphorylation in Hedgehog signaling
    ALAN ZHU; Fiscal Year: 2010
    ....
  67. Reversible phosphorylation in Hedgehog signaling
    ALAN ZHU; Fiscal Year: 2009
    ....
  68. CORE--SCIENTIFIC CORE
    ANDREW CZERNIK; Fiscal Year: 2000
    ....
  69. CORE--SCIENTIFIC CORE
    ANDREW CZERNIK; Fiscal Year: 1999
    ....
  70. Cellular Targets of Polyomavirus Tumor Antigens
    DAVID PALLAS; Fiscal Year: 2009
    ..Drugs that function at the level of this new target would likely be less toxic, and therefore more useful for anti-cancer therapy. ..
  71. Cellular Targets of Polyomavirus Tumor Antigens
    DAVID PALLAS; Fiscal Year: 2009
    ..Drugs that function at the level of this new target would likely be less toxic, and therefore more useful for anti-cancer therapy. ..
  72. A Testis Specific Regulatory Subunit of Protein Phosphatase-1
    ANN SPERRY; Fiscal Year: 2009
    ..This research will examine the importance of a testis-specific regulatory protein in spermatogenesis and will provide insight into possible remedies for male infertility as well as possible strategies for contraceptive design. ..