phosphodiesterase i

Summary

Summary: A phosphoric diester hydrolase that removes 5'-nucleotides from the 3'-hydroxy termini of 3'-hydroxy-terminated OLIGONUCLEOTIDES. It has low activity towards POLYNUCLEOTIDES and the presence of 3'-phosphate terminus on the substrate may inhibit hydrolysis.

Top Publications

  1. ncbi Regulation and biological activities of the autotaxin-LPA axis
    Laurens A van Meeteren
    Division of Cellular Biochemistry, Centre for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, The Netherlands
    Prog Lipid Res 46:145-60. 2007
  2. doi Gene expression profiles of lysophosphatidic acid-related molecules in the prostate: relevance to prostate cancer and benign hyperplasia
    Yu Zeng
    Department of Urology, Kagawa University Faculty of Medicine, Kita gun, Kagawa, Japan
    Prostate 69:283-92. 2009
  3. ncbi Identification of human plasma lysophospholipase D, a lysophosphatidic acid-producing enzyme, as autotaxin, a multifunctional phosphodiesterase
    Akira Tokumura
    Faculty of Pharmaceutical Sciences, The University of Tokushima, Tokushima 770 8505, Japan
    J Biol Chem 277:39436-42. 2002
  4. ncbi The hydrolysis of lysophospholipids and nucleotides by autotaxin (NPP2) involves a single catalytic site
    Rik Gijsbers
    Afdeling Biochemie, Faculteit Geneeskunde, University of Leuven, Herestraat 49, B 3000 Leuven, Belgium
    FEBS Lett 538:60-4. 2003
  5. doi Expression of autotaxin and lysophosphatidic acid receptors increases mammary tumorigenesis, invasion, and metastases
    Shuying Liu
    Department of Systems Biology, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Cancer Cell 15:539-50. 2009
  6. ncbi ATX-LPA receptor axis in inflammation and cancer
    Shuying Liu
    Department of Systems Biology, University of Texas M D Anderson Cancer Center, Houston, TX, USA
    Cell Cycle 8:3695-701. 2009
  7. pmc Autotaxin expression and its connection with the TNF-alpha-NF-kappaB axis in human hepatocellular carcinoma
    Jian Min Wu
    Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Mol Cancer 9:71. 2010
  8. doi Autotaxin
    Jean A Boutin
    Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, Croissy sur Seine, France
    Cell Mol Life Sci 66:3009-21. 2009
  9. pmc Phospholipase Cgamma activation drives increased production of autotaxin in endothelial cells and lysophosphatidic acid-dependent regression
    Eunok Im
    Schepens Eye Research Institute, Harvard Medical School, 20 Staniford St, Boston, MA 02114, USA
    Mol Cell Biol 30:2401-10. 2010
  10. pmc Autotaxin is released from adipocytes, catalyzes lysophosphatidic acid synthesis, and activates preadipocyte proliferation. Up-regulated expression with adipocyte differentiation and obesity
    Gilles Ferry
    Institut de Recherche Servier, Centre de Recherche de Croissy, 78290 Croissy sur Seine, France
    J Biol Chem 278:18162-9. 2003

Research Grants

  1. ENZYMOLOGY OF RNA PROCESSING ENZYMES
    Carol Fierke; Fiscal Year: 2000
  2. Quality Control of Protein Translation
    A Wali Karzai; Fiscal Year: 2009
  3. STRUCTURE/FUNCTION OF DNA POLYMERASE I OF E COLI
    NIGEL GRINDLEY; Fiscal Year: 2002
  4. ENZYMATIC MECHANISMS OF DNA SYNTHESIS
    ROBERT BAMBARA; Fiscal Year: 1993
  5. MOLECULAR GENETICS OF HSV DNA POLYMERASE GENE
    Donald Coen; Fiscal Year: 1993
  6. DNA-PROTEIN COMPLEX OF BACILLUS SUBTILIS PHAGE 029
    Margarita Salas; Fiscal Year: 1991
  7. p53 in Cellular Response to ROS-Mediated DNA Damage
    Peng Huang; Fiscal Year: 2007
  8. CRYSTALLOGRAPHIC STUDIES OF DNA REPLICATION PROTEINS
    Tom Ellenberger; Fiscal Year: 2000
  9. DNA REPAIR MECHANISMS IN USTILAGO MAYDIS
    William Holloman; Fiscal Year: 1999
  10. P53 AND REPAIR OF DNA DAMAGE BY ANTICANCER DRUGS
    Peng Huang; Fiscal Year: 2002

Detail Information

Publications177 found, 100 shown here

  1. ncbi Regulation and biological activities of the autotaxin-LPA axis
    Laurens A van Meeteren
    Division of Cellular Biochemistry, Centre for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, The Netherlands
    Prog Lipid Res 46:145-60. 2007
    ....
  2. doi Gene expression profiles of lysophosphatidic acid-related molecules in the prostate: relevance to prostate cancer and benign hyperplasia
    Yu Zeng
    Department of Urology, Kagawa University Faculty of Medicine, Kita gun, Kagawa, Japan
    Prostate 69:283-92. 2009
    ..To elucidate gene expression profiles of lysophosphatidic acid (LPA)-related molecules in cancer, pre-cancerous lesion, and benign hyperplasia of the prostate...
  3. ncbi Identification of human plasma lysophospholipase D, a lysophosphatidic acid-producing enzyme, as autotaxin, a multifunctional phosphodiesterase
    Akira Tokumura
    Faculty of Pharmaceutical Sciences, The University of Tokushima, Tokushima 770 8505, Japan
    J Biol Chem 277:39436-42. 2002
    ....
  4. ncbi The hydrolysis of lysophospholipids and nucleotides by autotaxin (NPP2) involves a single catalytic site
    Rik Gijsbers
    Afdeling Biochemie, Faculteit Geneeskunde, University of Leuven, Herestraat 49, B 3000 Leuven, Belgium
    FEBS Lett 538:60-4. 2003
    ..In comparing the substrate specificity of NPP2 and the closely related NPP1 and NPP3, we found that only NPP2 displayed a lysophospholipase D activity, whereas NPP1 and NPP3 had a much higher nucleotide pyrophosphatase activity...
  5. doi Expression of autotaxin and lysophosphatidic acid receptors increases mammary tumorigenesis, invasion, and metastases
    Shuying Liu
    Department of Systems Biology, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Cancer Cell 15:539-50. 2009
    ..Thus, ATX and LPA receptors can contribute to the initiation and progression of breast cancer...
  6. ncbi ATX-LPA receptor axis in inflammation and cancer
    Shuying Liu
    Department of Systems Biology, University of Texas M D Anderson Cancer Center, Houston, TX, USA
    Cell Cycle 8:3695-701. 2009
    ....
  7. pmc Autotaxin expression and its connection with the TNF-alpha-NF-kappaB axis in human hepatocellular carcinoma
    Jian Min Wu
    Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Mol Cancer 9:71. 2010
    ..Both ATX and LPA have been shown to be involved in many cancers. However, the functional role of ATX and the regulation of ATX expression in human hepatocellular carcinoma (HCC) remain elusive...
  8. doi Autotaxin
    Jean A Boutin
    Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, Croissy sur Seine, France
    Cell Mol Life Sci 66:3009-21. 2009
    ..The literature provides observations that may aid in redesigning experiments to validate autotaxin as a potential oncology target...
  9. pmc Phospholipase Cgamma activation drives increased production of autotaxin in endothelial cells and lysophosphatidic acid-dependent regression
    Eunok Im
    Schepens Eye Research Institute, Harvard Medical School, 20 Staniford St, Boston, MA 02114, USA
    Mol Cell Biol 30:2401-10. 2010
    ..These findings are likely to potentiate ongoing efforts to prevent, manage, and eradicate numerous angiogenesis-based diseases such as proliferative diabetic retinopathy and solid tumors...
  10. pmc Autotaxin is released from adipocytes, catalyzes lysophosphatidic acid synthesis, and activates preadipocyte proliferation. Up-regulated expression with adipocyte differentiation and obesity
    Gilles Ferry
    Institut de Recherche Servier, Centre de Recherche de Croissy, 78290 Croissy sur Seine, France
    J Biol Chem 278:18162-9. 2003
    ..Up-regulations of ATX expression with adipocyte differentiation and genetic obesity suggest a possible involvement of this released protein in the development of adipose tissue and obesity-associated pathologies...
  11. doi Murine and human autotaxin alpha, beta, and gamma isoforms: gene organization, tissue distribution, and biochemical characterization
    Adeline Giganti
    Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine, BP 84225, 31432 Toulouse Cedex 4, France
    J Biol Chem 283:7776-89. 2008
    ..Finally, the specificity of the isoforms toward a large family of lysophosphatidylcholines is reported. This study is the first complete description of the reported autotaxin isoforms...
  12. doi S32826, a nanomolar inhibitor of autotaxin: discovery, synthesis and applications as a pharmacological tool
    Gilles Ferry
    Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, Croissy sur Seine, France
    J Pharmacol Exp Ther 327:809-19. 2008
    ..S32826 is the first reported inhibitor of autotaxin with an IC(50) in the nanomolar range that can be used to validate the role of autotaxin in various pathologies in cellular models...
  13. ncbi cDNA cloning of the human tumor motility-stimulating protein, autotaxin, reveals a homology with phosphodiesterases
    J Murata
    Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 269:30479-84. 1994
    ..ATX, like PC-1, was found to hydrolyze the type I phosphodiesterase substrate p-nitrophenyl thymidine-5'-monophosphate. Autotaxin now defines a novel motility-regulating function for this class of ecto/exo-enzymes...
  14. doi Two pathways for lysophosphatidic acid production
    Junken Aoki
    Graduate School of Pharmaceutical Sciences, Tohoku University, 6 3, Aobayama, Aoba ku, Sendai, 980 8578, Japan
    Biochim Biophys Acta 1781:513-8. 2008
    ..One PA-selective PLA1 called mPA-PLA1alpha/LIPH is specifically expressed in hair follicles, where it has a critical role in hair growth by producing LPA through a novel LPA receptor called P2Y5...
  15. ncbi The ins and outs of lysophosphatidic acid signaling
    Wouter H Moolenaar
    Division of Cellular Biochemistry and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan, Amsterdam, The Netherlands
    Bioessays 26:870-81. 2004
    ..Exogenous phospholipases D can also produce LPA, which may contribute to their toxicity. Here we review recent progress in our understanding of LPA bioactivity, signaling and synthesis...
  16. ncbi Proteolytic maturation and activation of autotaxin (NPP2), a secreted metastasis-enhancing lysophospholipase D
    Silvia Jansen
    Division of Biochemistry, Flanders Interuniversity Institute for Biotechnology, Faculty of Medicine, K U Leuven, B 3000 Leuven, Belgium
    J Cell Sci 118:3081-9. 2005
    ..These novel insights in the maturation of NPP2 have also implications for the development of NPP2 inhibitors as potential anti-cancer agents...
  17. pmc Lysophospholipids in the limelight: autotaxin takes center stage
    Wouter H Moolenaar
    Division of Cellular Biochemistry, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Cell Biol 158:197-9. 2002
    ..This result is surprising, as there was previously no indication that autotaxin could act as a phospholipase...
  18. ncbi Phenolic glycosides from Symplocos racemosa: natural inhibitors of phosphodiesterase I
    Viqar Uddin Ahmad
    H E J Research Institute of Chemistry, International Center for Chemical Sciences, University of Karachi, Karachi 75270, Pakistan
    Phytochemistry 63:217-20. 2003
    ..The structure elucidation of the isolated compounds was based primarily on 1D- and 2D-NMR analysis, including COSY, HMQC, and HMBC correlations. The compound 1 and 2 showed inhibitory activity against snake venom phosphodiesterase I.
  19. pmc Evaluating dual activity LPA receptor pan-antagonist/autotaxin inhibitors as anti-cancer agents in vivo using engineered human tumors
    Xiaoyu Xu
    Department of Medicinal Chemistry and The Center for Therapeutic Biomaterials, The University of Utah, Suite 205, Salt Lake City, UT 84108 1257, USA
    Prostaglandins Other Lipid Mediat 89:140-6. 2009
    ....
  20. pmc Therapeutic potential of autotaxin/lysophospholipase d inhibitors
    Lorenzo Federico
    Division of Cardiovascular Medicine, The Gill Heart Institute, 900 S Limestone Street, 326 CTW Building, Lexington, KY 40536 0200, USA
    Curr Drug Targets 9:698-708. 2008
    ....
  21. ncbi Inhibition of autotaxin by lysophosphatidic acid and sphingosine 1-phosphate
    Laurens A van Meeteren
    Division of Cellular Biochemistry and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 280:21155-61. 2005
    ....
  22. ncbi Mechanisms of lysophosphatidic acid production
    Junken Aoki
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Semin Cell Dev Biol 15:477-89. 2004
    ..However, apparently certain extracellular phospholipases such as secretory PLA2 (sPLA2-IIA), membrane-associated PA-selective PLA1 (mPA-PLA1), lecithin-cholesterol acyltransferase (LCAT), and lysoPLD are involved in LPA production...
  23. pmc Autotaxin, an ectoenzyme that produces lysophosphatidic acid, promotes the entry of lymphocytes into secondary lymphoid organs
    Hidenobu Kanda
    Department of Anatomy, Program in Immunology, Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143, USA
    Nat Immunol 9:415-23. 2008
    ..Our results support the idea of a new and general step in the homing cascade in which the ectoenzyme ATX facilitates the entry of lymphocytes into lymphoid organs...
  24. ncbi Lysophosphatidic acid inhibits adipocyte differentiation via lysophosphatidic acid 1 receptor-dependent down-regulation of peroxisome proliferator-activated receptor gamma2
    Marie Francoise Simon
    INSERM U586, Institut Louis Bugnard, Rangueil Hospital, BP84225, 31432 Toulouse, Cedex 4, France
    J Biol Chem 280:14656-62. 2005
    ..The local production of LPA may exert a tonic inhibitory effect on the development of adipose tissue...
  25. ncbi Lysophosphatidic acid signaling: how a small lipid does big things
    Celine Luquain
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27699 7090, USA
    Trends Biochem Sci 28:377-83. 2003
  26. doi Identification of small-molecule inhibitors of autotaxin that inhibit melanoma cell migration and invasion
    Lauren P Saunders
    Department of Molecular Biophysics and Biochemistry, Yale University, P O Box 208114, New Haven, CT 06520 8114, USA
    Mol Cancer Ther 7:3352-62. 2008
    ..These studies suggest that ATX is an approachable molecular target for the rational design of chemotherapeutic agents directed against malignant melanoma...
  27. pmc Potential involvement of adipocyte insulin resistance in obesity-associated up-regulation of adipocyte lysophospholipase D/autotaxin expression
    J Boucher
    INSERM U586, Institut Louis Bugnard, Rangueil Hospital, TSA 50032, 31059, Toulouse Cedex 9, France
    Diabetologia 48:569-77. 2005
    ..The aim of the present study was to depict the physiopathological and cellular mechanisms involved in regulation of adipocyte autotaxin expression...
  28. pmc Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production
    Makiko Umezu-Goto
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Cell Biol 158:227-33. 2002
    ..It also provides potential novel targets for therapy of pathophysiological states including cancer...
  29. ncbi Phosphodiesterase-Ialpha/autotaxin: a counteradhesive protein expressed by oligodendrocytes during onset of myelination
    Michael A Fox
    Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, VA 23298, USA
    Mol Cell Neurosci 23:507-19. 2003
    ..Thus, the release of PD-Ialpha/ATX may be critically involved in the regulation of the initial stages of myelination, i.e., oligodendrocyte remodeling, via modulation of oligodendrocyte-ECM interactions in a matricellular fashion...
  30. ncbi Integrin alpha6beta4 promotes expression of autotaxin/ENPP2 autocrine motility factor in breast carcinoma cells
    Min Chen
    Department of Surgery and the Sealy Center for Cancer Cell Biology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555, USA
    Oncogene 24:5125-30. 2005
    ..These observations highlight for the first time a mechanism by which NFAT transcription factors can facilitate an invasive and motile phenotype downstream of integrin alpha6beta4 signaling...
  31. ncbi Biochemical evidence for an ecto alkaline phosphodiesterase I in human airways
    M Picher
    Cystic Fibrosis Pulmonary Research and Treatment Center, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Am J Respir Cell Mol Biol 23:255-61. 2000
    ..5'-monophosphate p-nitrophenyl ester and adenosine diphosphate (ADP)- ribose, substrates of ecto alkaline phosphodiesterase I (PDE I) activities, were also hydrolyzed by the apical surface of airway epithelia...
  32. ncbi Autotaxin (NPP-2), a metastasis-enhancing motogen, is an angiogenic factor
    S W Nam
    Laboratory of Pathology, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 61:6938-44. 2001
    ....
  33. ncbi Microarray analysis identifies Autotaxin, a tumour cell motility and angiogenic factor with lysophospholipase D activity, as a specific target of cell transformation by v-Jun
    Elizabeth J Black
    Beatson Institute for Cancer Research, Cancer Research Campaign Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    Oncogene 23:2357-66. 2004
    ..ATX has been implicated in both angiogenesis and invasion, and could therefore play an important role in tumorigenesis by v-Jun in vivo...
  34. doi Autotaxin protects MCF-7 breast cancer and MDA-MB-435 melanoma cells against Taxol-induced apoptosis
    N Samadi
    Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada
    Oncogene 28:1028-39. 2009
    ..Our results support the hypothesis that therapeutic inhibition of ATX activity could improve the efficacy of Taxol as a chemotherapeutic agent for cancer treatment...
  35. pmc Synthesis and structure-activity relationships of tyrosine-based inhibitors of autotaxin (ATX)
    James E East
    University of Virginia, Department of Chemistry, Charlottesville, VA 22904, USA
    Bioorg Med Chem Lett 20:7132-6. 2010
    ..Here we describe the SAR of ATX inhibitor, VPC8a202, and apply this SAR knowledge towards developing a high potency inhibitor. We found that electron density in the pyridine region greatly influences activity of our inhibitors at ATX...
  36. doi Development of an activity-based probe for autotaxin
    Silvia Cavalli
    Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Chembiochem 11:2311-7. 2010
    ..Our activity-based reporter will be useful for monitoring ATX activity in biological fluids and for inhibitor screening...
  37. doi Discovery and optimization of boronic acid based inhibitors of autotaxin
    Harald M H G Albers
    Division of Cell Biology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    J Med Chem 53:4958-67. 2010
    ..The most potent of these compounds inhibits ATX-mediated LPC hydrolysis in the nanomolar range (IC(50) = 6 nM). The finding that ATX can be targeted by boronic acids may aid the development of ATX inhibitors for therapeutic use...
  38. pmc Boronic acid-based inhibitor of autotaxin reveals rapid turnover of LPA in the circulation
    Harald M H G Albers
    Division of Cell Biology, Netherlands Proteomics Centre, Centre of Biomedical Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    Proc Natl Acad Sci U S A 107:7257-62. 2010
    ..Thus, boronic acid-based small molecules hold promise as candidate drugs to target ATX...
  39. doi Rapid clearance of the circulating metastatic factor autotaxin by the scavenger receptors of liver sinusoidal endothelial cells
    Silvia Jansen
    Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, University of Leuven, B 3000 Leuven, Belgium
    Cancer Lett 284:216-21. 2009
    ..Our finding that circulating NPP2 has a rapid turnover has important implications for its development as an anti-cancer target...
  40. pmc Autotaxin signaling via lysophosphatidic acid receptors contributes to vascular endothelial growth factor-induced endothelial cell migration
    Malgorzata M Ptaszynska
    Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892 1500, USA
    Mol Cancer Res 8:309-21. 2010
    ..These data further suggest that ATX could be a prognostic factor or a target for therapeutic intervention in several cancers...
  41. pmc Autotaxin, a secreted lysophospholipase D, is essential for blood vessel formation during development
    Laurens A van Meeteren
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Mol Cell Biol 26:5015-22. 2006
    ....
  42. doi Autotaxin delays apoptosis induced by carboplatin in ovarian cancer cells
    Susanne Vidot
    Section of Medicine, The Institute of Cancer Research, 15 Cotswold Road, Sutton, SM2 5NG, United Kingdom
    Cell Signal 22:926-35. 2010
    ..These results indicate that autotaxin delays apoptosis induced by carboplatin in ovarian cancer cells...
  43. ncbi Autotaxin is overexpressed in glioblastoma multiforme and contributes to cell motility of glioblastoma by converting lysophosphatidylcholine to lysophosphatidic acid
    Yasuhiro Kishi
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033
    J Biol Chem 281:17492-500. 2006
    ....
  44. pmc Inhibition of autotaxin production or activity blocks lysophosphatidylcholine-induced migration of human breast cancer and melanoma cells
    Cristoforo G Gaetano
    Department of Biochemistry, Signal Transduction Research Group, University of Alberta, Edmonton, Alberta, Canada T6G 2S2
    Mol Carcinog 48:801-9. 2009
    ..We conclude that inhibiting autotaxin production or activity could provide a beneficial adjuvant to chemotherapy for preventing tumor growth and metastasis in patients with high autotaxin expression in their tumors...
  45. doi Pharmacophore development and application toward the identification of novel, small-molecule autotaxin inhibitors
    E Jeffrey North
    Department of Chemistry, The University of Memphis, Memphis, Tennessee 38152, USA
    J Med Chem 53:3095-105. 2010
    ..Five of these compounds had IC(50) < 1.5 microM and the most potent compound possessed a K(i) of 271 nM...
  46. ncbi Expression, regulation and function of autotaxin in thyroid carcinomas
    Astrid Kehlen
    Institute of Medical Immunology, University of Halle Wittenberg, Halle, Germany
    Int J Cancer 109:833-8. 2004
    ..Further studies are needed to provide insight into the role of ATX in the normal and neoplastic thyroid gland...
  47. ncbi Autotaxin is an exoenzyme possessing 5'-nucleotide phosphodiesterase/ATP pyrophosphatase and ATPase activities
    T Clair
    Laboratory of Pathology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 272:996-1001. 1997
    ....
  48. ncbi Autotaxin hydrolyzes sphingosylphosphorylcholine to produce the regulator of migration, sphingosine-1-phosphate
    Timothy Clair
    Laboratory of Pathology, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 63:5446-53. 2003
    ....
  49. pmc Carba analogs of cyclic phosphatidic acid are selective inhibitors of autotaxin and cancer cell invasion and metastasis
    Daniel L Baker
    Department of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA
    J Biol Chem 281:22786-93. 2006
    ..Here we show that 3-carba analogs of cPA lack significant agonist activity at LPA receptors yet are potent inhibitors of ATX activity, LPA production, and A2058 melanoma cell invasion in vitro and B16F10 melanoma cell metastasis in vivo...
  50. pmc Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells
    Malgorzata M Ptaszynska
    Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892 1500, USA
    Mol Cancer Res 6:352-63. 2008
    ..Because LPA was previously shown to increase VEGF expression in ovarian cancer, our data suggest a positive feedback loop involving VEGF, ATX, and its product LPA that could affect tumor progression in ovarian cancer cells...
  51. pmc Synthesis and properties of 4'-ThioDNA: unexpected RNA-like behavior of 4'-ThioDNA
    Naonori Inoue
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Nucleic Acids Res 34:3476-83. 2006
    ..Since a 4'-thioDNA duplex competitively inhibited the hydrolysis of an RNA duplex by RNase V1, it was not only the physical properties but also this biological data suggested that a 4'-thioDNA duplex has an RNA-like structure...
  52. ncbi Is autotaxin (ENPP2) the link between hepatitis C and hepatocellular cancer?
    Amanda B Cooper
    Department of General Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    J Gastrointest Surg 11:1628-34; discussion 1634-5. 2007
    ..The study objective was to investigate differences in gene expression between patients with Hepatitis C (+/- cancer) and normal that might explain the increased cancer risk...
  53. ncbi Phosphodiesterase-Ialpha/autotaxin (PD-Ialpha/ATX): a multifunctional protein involved in central nervous system development and disease
    Jameel Dennis
    Department of Anatomy and Neurobiology, Virginia Commonwealth University Medical Center, Richmond, 23298, USA
    J Neurosci Res 82:737-42. 2005
    ..This Mini-Review describes PD-Ialpha/ATX's multifunctional roles in the CNS and discusses their potential contributions to CNS development and pathology...
  54. ncbi Functional invalidation of the autotaxin gene by a single amino acid mutation in mouse is lethal
    Gilles Ferry
    Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 125 Chemin de Ronde, 78290 Croissy sur Seine, France
    FEBS Lett 581:3572-8. 2007
    ..To our knowledge, the present work is the first showing such a lethality in any gene after a point mutation in an enzyme catalytic site...
  55. pmc Fluorogenic phospholipid substrate to detect lysophospholipase D/autotaxin activity
    Colin G Ferguson
    Echelon Biosciences Inc, Salt Lake City, Utah 84108, USA
    Org Lett 8:2023-6. 2006
    ..The substrates (FS-2 and FS-3) show significant increases in fluorescence when treated with recombinant ATX and have potential applications in screening for this emerging drug target...
  56. ncbi Autotaxin expression is enhanced in frontal cortex of Alzheimer-type dementia patients
    Ken Umemura
    National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan
    Neurosci Lett 400:97-100. 2006
    ..These observations suggest that expression of the autotaxin gene and cell signaling by lysophosphatidic acid may be involved in the pathology of ATD, and that this cell signaling pathway may be a potential target of treatments for ATD...
  57. ncbi Autotaxin stabilizes blood vessels and is required for embryonic vasculature by producing lysophosphatidic acid
    Masayuki Tanaka
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biol Chem 281:25822-30. 2006
    ..The present study revealed a previously unassigned role of ATX in stabilizing vessels through novel LPA signaling pathways...
  58. ncbi Genomic structure and promoter analysis of the ecto-phosphodiesterase I gene (PDNP3) expressed in glial cells
    K Andoh
    Department of Pediatrics, Kobe University School of Medicine, Japan
    Biochim Biophys Acta 1446:213-24. 1999
    PDNP (phosphodiesterase I/nucleotide pyrophosphatase) is one of a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. PDNP possesses ATPase (EC 3.6.1.3) and ATP pyrophosphatase (EC 3.6.1.8) activities...
  59. pmc Substrate-specifying determinants of the nucleotide pyrophosphatases/phosphodiesterases NPP1 and NPP2
    Anisoara Cimpean
    Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, Herestraat 49, B 3000 Leuven, Belgium
    Biochem J 381:71-7. 2004
    ..Our data suggest that the distinct activities of NPP1 and NPP2 stem from multiple differences throughout the polypeptide chain...
  60. pmc Reduced expression of autotaxin predicts survival in uveal melanoma
    Arun D Singh
    Department of Ophthalmic Oncology, Cole Eye Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Br J Ophthalmol 91:1385-92. 2007
    ..In an effort to identify patients with uveal melanoma at high risk of metastasis, the authors undertook correlation of gene expression profiles with histopathology data and tumour-related mortality...
  61. ncbi Synthesis and biological evaluation of phosphonate derivatives as autotaxin (ATX) inhibitors
    Peng Cui
    Department of Chemistry, University of Virginia, McCormick Road, Charlottesville, VA 22904, USA
    Bioorg Med Chem Lett 17:1634-40. 2007
    ..With this study we have generated a series of beta-keto and beta-hydroxy phosphonate derivatives of LPA, some of which are potent ATX inhibitors...
  62. pmc Autotaxin, a synthetic enzyme of lysophosphatidic acid (LPA), mediates the induction of nerve-injured neuropathic pain
    Makoto Inoue
    Division of Molecular Pharmacology and Neuroscience, Nagasaki University, Graduate School of Biomedical Sciences, 1 14 Bunkyo machi, Nagasaki 852 8521, Japan
    Mol Pain 4:6. 2008
    ..Therefore, targeted inhibition of ATX-mediated LPA biosynthesis as well as LPA1 receptor and its downstream pathways may represent a novel way to prevent nerve injury-induced neuropathic pain...
  63. doi Serum autotaxin measurement in haematological malignancies: a promising marker for follicular lymphoma
    Akiko Masuda
    Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
    Br J Haematol 143:60-70. 2008
    ..Our results suggest that the serum ATX antigen level may be a promising and novel marker for FL...
  64. pmc Dual activity lysophosphatidic acid receptor pan-antagonist/autotaxin inhibitor reduces breast cancer cell migration in vitro and causes tumor regression in vivo
    Honglu Zhang
    Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah 84108, USA
    Cancer Res 69:5441-9. 2009
    ..All treatments significantly reduced tumor burden, and BrP-LPA was superior to Taxol in reducing blood vessel density in tumors. Moreover, both the anti- and syn-BrP-LPA significantly reduced tumors at 3 mg/kg...
  65. ncbi Stimulation of tumor cell motility linked to phosphodiesterase catalytic site of autotaxin
    H Y Lee
    Laboratory of Pathology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 271:24408-12. 1996
    ....
  66. ncbi Site-directed mutations in the tumor-associated cytokine, autotaxin, eliminate nucleotide phosphodiesterase, lysophospholipase D, and motogenic activities
    Eunjin Koh
    Laboratory of Pathology, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 63:2042-5. 2003
    ....
  67. pmc IL-1 beta- and IL-4-induced down-regulation of autotaxin mRNA and PC-1 in fibroblast-like synoviocytes of patients with rheumatoid arthritis (RA)
    A Kehlen
    Institute of Medical Immunology and Institute of Physiological Chemistry, Martin Luther University, Halle, Germany
    Clin Exp Immunol 123:147-54. 2001
    ..Further investigations will deal with the regulation of ATX protein expression as well as with the function of ATX in RA...
  68. pmc Lysophosphatidic acid-3 receptor-mediated feed-forward production of lysophosphatidic acid: an initiator of nerve injury-induced neuropathic pain
    Lin Ma
    Nagasaki University Graduate School of Biomedical Sciences, Japan
    Mol Pain 5:64. 2009
    ..We previously reported that intrathecal injection of lysophosphatidylcholine (LPC) induced neuropathic pain through activation of the lysophosphatidic acid (LPA)-1 receptor, possibly via conversion to LPA by autotaxin (ATX)...
  69. doi Simultaneous stimulation of spinal NK1 and NMDA receptors produces LPC which undergoes ATX-mediated conversion to LPA, an initiator of neuropathic pain
    Makoto Inoue
    Division of Molecular Pharmacology and Neuroscience, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
    J Neurochem 107:1556-65. 2008
    ..These findings suggest that simultaneous intense stimulation of neurokinin 1 and NMDA receptors in the spinal dorsal horn triggers lysophosphatidic acid production from lysophosphatidylcholine through extracellular autotaxin...
  70. doi Evidence for de novo synthesis of lysophosphatidic acid in the spinal cord through phospholipase A2 and autotaxin in nerve injury-induced neuropathic pain
    Lin Ma
    Division of Molecular Pharmacology and Neuroscience, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
    J Pharmacol Exp Ther 333:540-6. 2010
    ..These findings provide evidence that PLA2- and ATX-mediated de novo LPA production in the early phase is involved in nerve injury-induced neuropathic pain...
  71. doi Lipopolysaccharide induces autotaxin expression in human monocytic THP-1 cells
    Song Li
    The Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education, Institute of Cell Biology, College of Life Sciences, Beijing Normal University, No 19 Xinjiekouwai Avenue, Beijing 100875, China
    Biochem Biophys Res Commun 378:264-8. 2009
    ....
  72. ncbi Cloning, chromosomal localization, and tissue expression of autotaxin from human teratocarcinoma cells
    H Y Lee
    Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochem Biophys Res Commun 218:714-9. 1996
    ..the teratocarcinoma autotaxin has 94% identity to the melanoma-derived protein, 90% identity to rat brain phosphodiesterase I/nucleotide pyrophosphatase (PD-I alpha), and 44% identity to the plasma cell membrane marker PC-I...
  73. pmc Identification of nucleotide pyrophosphatase/alkaline phosphodiesterase I activity associated with the mouse plasma cell differentiation antigen PC-1
    N F Rebbe
    Washington University Medical Service, Veterans Affairs Medical Center, John Cochran Division, St Louis, MO 63106
    Proc Natl Acad Sci U S A 88:5192-6. 1991
    The protein responsible for both nucleotide pyrophosphatase (EC 3.6.1.9) and alkaline phosphodiesterase I (EC 3.1.4.1) activities was purified from MOPC 315 plasmacytoma cells...
  74. doi Lysophosphatidylcholine induces neuropathic pain through an action of autotaxin to generate lysophosphatidic acid
    M Inoue
    Division of Molecular Pharmacology and Neuroscience, Nagasaki University, Graduate School of Biomedical Sciences, 1 14 Bunkyo machi, Nagasaki, 852 8521, Japan
    Neuroscience 152:296-8. 2008
    ..These findings suggest that intrathecally-injected LPC is converted to LPA by ATX, and this LPA activates the LPA(1) receptor to initiate neuropathic pain...
  75. pmc Autotaxin/lysopholipase D and lysophosphatidic acid regulate murine hemostasis and thrombosis
    Zehra Pamuklar
    Division of Cardiovascular Medicine, The Gill Heart Institute, Lexington, Kentucky 40536 0200, USA
    J Biol Chem 284:7385-94. 2009
    ....
  76. doi Expression of autotaxin and acylglycerol kinase in prostate cancer: association with cancer development and progression
    Mohammed Ahmed Abdel Muneem Nouh
    Department of Urology, Kagawa University Faculty of Medicine, Miki cho, Kagawa, Japan
    Cancer Sci 100:1631-8. 2009
    ..In conclusion, LPA-producing enzymes (ATX and AGK) were frequently expressed in prostate cancer cells and precancerous HG-PIN. In particular, high expression levels of ATX were associated with both malignant potentials and poor outcomes...
  77. ncbi Serum lysophospholipase D/autotaxin may be a new nutritional assessment marker: study on prostate cancer patients
    Kazuhiro Nakamura
    Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan
    Ann Clin Biochem 44:549-56. 2007
    ..In this study, we evaluated the usefulness of lysoPLD/ATX activity as a diagnostic marker...
  78. pmc Production of lysophosphatidic acid in blister fluid: involvement of a lysophospholipase D activity
    Juliette Mazereeuw-Hautier
    Service de Dermatologie, Hopital Rangueil, Toulouse Cedex, France
    J Invest Dermatol 125:421-7. 2005
    ..Due to its ability to enhance keratinocyte migration, LPA in blister fluid could, via the LPA1-R, play an important role in re-epithelialization occurring after blister rupture...
  79. ncbi Secretion and lysophospholipase D activity of autotaxin by adipocytes are controlled by N-glycosylation and signal peptidase
    Jean Philippe Pradere
    INSERM, U586, Unité de Recherches sur les Obésités, Toulouse, F 31432, France
    Biochim Biophys Acta 1771:93-102. 2007
    ..In conclusion, the present work demonstrates the crucial role of N-glycosylation in secretion and activity of ATX. The present work also confirms the crucial role signal peptidase in secretion of ATX by adipocytes...
  80. ncbi Autotaxin (lysoPLD/NPP2) protects fibroblasts from apoptosis through its enzymatic product, lysophosphatidic acid, utilizing albumin-bound substrate
    Jaehwi Song
    Department of Pathology, College of Medicine and Microdissection Genomics Research Center, The Catholic University of Korea, Banpo dong number 505, Seocho Gu, Seoul 137 701, Republic of Korea
    Biochem Biophys Res Commun 337:967-75. 2005
    ..These findings provide novel insights into understanding the functions of ATX as a key regulator of bioactive phospholipids and suggest interventions to correct dysfunction in conditions of tumor cell growth and metastasis...
  81. ncbi Suppression of lysophosphatidic acid and lysophosphatidylcholine formation in the plasma in vitro: proposal of a plasma sample preparation method for laboratory testing of these lipids
    Kazuhiro Nakamura
    Department of Clinical Laboratory, University of Tokyo Hospital, Tokyo 113 8655, Japan
    Anal Biochem 367:20-7. 2007
    ..Thus, although the plasma levels of LPA and LPC can be easily altered, laboratory testing of these important bioactive lipids for clinical purposes may be conducted reliably if the samples are prepared under stringent conditions...
  82. ncbi Submucosal connective tissue-type mast cells contribute to the production of lysophosphatidic acid (LPA) in the gastrointestinal tract through the secretion of autotaxin (ATX)/lysophospholipase D (lysoPLD)
    Ken Mori
    Department of Surgical Oncology, University of Tokyo Graduate School of Medicine, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    Virchows Arch 451:47-56. 2007
    ..Our data suggest that submucosal MC play significant roles in various aspects of pathophysiology in the gastrointestinal tract by locally providing bioactive LPA through the production of ATX/lysoPLD...
  83. ncbi Autotaxin is an N-linked glycoprotein but the sugar moieties are not needed for its stimulation of cellular motility
    M L Stracke
    Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Melanoma Res 5:203-9. 1995
    ..Therefore, the carbohydrate side chains on autotaxin are not necessary to stimulate motility; however, they may still play a role in folding, secretion or maintenance of the active conformation of the protein...
  84. ncbi Peritoneal fluids from patients with certain gynecologic tumor contain elevated levels of bioactive lysophospholipase D activity
    Akira Tokumura
    Department of Health Chemistry, Institute of Health Biosciences, University of Tokushima Graduate School, 1 78 1 Shomachi, Tokushima, Japan
    Life Sci 80:1641-9. 2007
    ..Our results suggest that increased lysoPLD activity in peritoneal fluid from patients with certain gynecologic tumors might be relevant to its potential of tumor progression...
  85. ncbi Measurement of lysophospholipase D/autotaxin activity in human serum samples
    Kazuhiro Nakamura
    Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan
    Clin Biochem 40:274-7. 2007
    ..Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing...
  86. ncbi Cyclic phosphatidic acid is produced by autotaxin in blood
    Satomi Tsuda
    Department of Biology, Faculty of Science, Ochanomizu University, Tokyo 112 8610, Japan
    J Biol Chem 281:26081-8. 2006
    ..These results indicate that the generation of cPA and LPA in serum is mainly attributed to autotaxin...
  87. ncbi [Secretion and role of autotaxin and lysophosphatidic acid in adipose tissue]
    Jean Sébastien Saulnier-Blache
    IFR31 Institut Louis Bugnard, BP 84225, INSERM U586, Unité de Recherches sur les Obésités, 31432 Toulouse 4, France
    J Soc Biol 200:77-81. 2006
    ..A possible contribution of ATX and LPA as paracrine regulators of adipogenesis and obesity associated diabetes is proposed...
  88. ncbi Induction of autotaxin by the Epstein-Barr virus promotes the growth and survival of Hodgkin lymphoma cells
    Karl R N Baumforth
    CRUK Institute for Cancer Studies, The Medical School, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
    Blood 106:2138-46. 2005
    ....
  89. ncbi Measurement of autotaxin/lysophospholipase D activity
    Andrew J Morris
    Division of Cardiovascular Medicine, The Gill Heart Institute, University of Kentucky College of Medicine, Lexington, Kentucky, USA
    Methods Enzymol 434:89-104. 2007
    ..This chapter describes methods for determining the activity of recombinant autotaxin/lysoPLD using radiolabeled and fluorogenic substrates...
  90. ncbi Degradation of extracellular ATP by the retinal pigment epithelium
    David Reigada
    Dept of Physiology, University of Pennsylvania, Philadelphia, PA 19104 6085, USA
    Am J Physiol Cell Physiol 289:C617-24. 2005
    ..These results suggest that extracellular degradation of ATP in subretinal space can result in the production of ADP. This ADP can stimulate P2Y receptors and augment Ca(2+) signaling in the RPE...
  91. ncbi Retinoic acid-induced expression of autotaxin in N-myc-amplified neuroblastoma cells
    J Dufner-Beattie
    Department of Human Genetics, University of Utah, Salt Lake City, Utah
    Mol Carcinog 30:181-9. 2001
    ..ATX induction by retinoic acid was due to increased transcription and required new protein synthesis...
  92. doi Scalable purification and characterization of the extracellular domain of human autotaxin from prokaryotic cells
    Arayo Haga
    Research Institute for Health and Environmental Science, Gifu Prefectural Government, 1 1, Naka Fudougaoka, Kakamigahara 504 0838, Japan
    Protein Expr Purif 59:9-17. 2008
    ..This is a first report for scalable purification of the ATX molecule and the rhATX S48 should be a good tool for immunization of anti-ATX or crystallographic analysis of ATX...
  93. ncbi Up-regulated expression of the phosphodiesterase nucleotide pyrophosphatase family member PC-1 is a marker and pathogenic factor for knee meniscal cartilage matrix calcification
    K Johnson
    VA Medical Center, San Diego, California 92161, USA
    Arthritis Rheum 44:1071-81. 2001
    ..Increased expression of PC-1 is both a marker and a potential pathogenic factor for knee meniscal cartilage matrix calcification...
  94. pmc Lysophosphatidic acid, LPA: a bad boy becomes good
    Lygia T Budnik
    Institute for Hormone and Fertility Research, University of Hamburg, Centrum for Innovative Medicine, Falkenried 88, D 20251 Hamburg, Germany
    Reprod Biol Endocrinol 1:37. 2003
  95. ncbi Enzymatic activation of autotaxin by divalent cations without EF-hand loop region involvement
    J Lee
    College of Medicine, Konyang University, Nonsan 320-711, South Korea
    Biochem Pharmacol 62:219-24. 2001
    ..However, the PDE activity of this same deletion mutant remained sensitive to augmentation by cations, strongly implying that cations exert their effect by interactions outside of the EF-hand loop region...
  96. ncbi The nucleoside triphosphate pyrophosphohydrolase isozyme PC-1 directly promotes cartilage calcification through chondrocyte apoptosis and increased calcium precipitation by mineralizing vesicles
    K Johnson
    VA Medical Center University of California San Diego, 92161, USA
    J Rheumatol 28:2681-91. 2001
    ..These results suggest a direct link between increased PC-1 expression and the pathogenesis of chondrocalcinosis...
  97. ncbi Autotaxin promotes motility via G protein-coupled phosphoinositide 3-kinase gamma in human melanoma cells
    Hoi Young Lee
    College of Medicine, Konyang University, 320 711, Nonsan, South Korea
    FEBS Lett 515:137-40. 2002
    ..Furthermore, stimulation of tumor cell motility by ATX was inhibited by catalytically inactive form of PI3Kgamma, strongly indicating the crucial role of PI3Kgamma for ATX-mediated motility in human melanoma cells..
  98. ncbi Cdc42 and Rac1 are necessary for autotaxin-induced tumor cell motility in A2058 melanoma cells
    In Duk Jung
    College of Medicine, Konyang University, 320 711, Nonsan, South Korea
    FEBS Lett 532:351-6. 2002
    ..Collectively, these data strongly indicate that Cdc42 and Rac1 are essential for ATX-induced tumor cell motility in A2058 melanoma cells, and that PAK and FAK might be also involved in the process...
  99. pmc Anticancer activity of FTY720: phosphorylated FTY720 inhibits autotaxin, a metastasis-enhancing and angiogenic lysophospholipase D
    Laurens A van Meeteren
    Division of Cellular Biochemistry and Center for Biomedical Genetics, The Netherlands Cancer Institute, 1066 Amsterdam, The Netherlands
    Cancer Lett 266:203-8. 2008
    ..After oral administration in mice, FTY720 (3mg/kg) significantly reduced plasma LPA levels. These results suggest that FTY720 may exert its anticancer effects, at least in part, by targeting the ATX-LPA axis...
  100. ncbi Lysophosphatidic acid and autotaxin stimulate cell motility of neoplastic and non-neoplastic cells through LPA1
    Kotaro Hama
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biol Chem 279:17634-9. 2004
    ..The present study suggests that ATX and LPA(1) represent potential targets for cancer therapy...
  101. pmc Autotaxin (ATX): a multi-functional and multi-modular protein possessing enzymatic lysoPLD activity and matricellular properties
    Larra M Yuelling
    Department of Anatomy and Neurobiology, Virginia Commonwealth University School of Medicine, PO Box 980709, Richmond, VA 23298, USA
    Biochim Biophys Acta 1781:525-30. 2008
    ..In this review, we summarize our current understanding of ATX's structure-function domains and discuss their contribution to the presently known main functional roles of ATX...

Research Grants70

  1. ENZYMOLOGY OF RNA PROCESSING ENZYMES
    Carol Fierke; Fiscal Year: 2000
    ..Furthermore, since RNase P is an essential bacterial enzyme, this enzyme may represent a feasible target for novel antibiotics. ..
  2. Quality Control of Protein Translation
    A Wali Karzai; Fiscal Year: 2009
    ..Ultimately, these insights will have implications for a better understanding of a variety of cellular processes, including control of gene expression, synthesis and degradation of proteins, and the targeted decay defective mRNAs. ..
  3. STRUCTURE/FUNCTION OF DNA POLYMERASE I OF E COLI
    NIGEL GRINDLEY; Fiscal Year: 2002
    ..abstract_text> ..
  4. ENZYMATIC MECHANISMS OF DNA SYNTHESIS
    ROBERT BAMBARA; Fiscal Year: 1993
    ....
  5. MOLECULAR GENETICS OF HSV DNA POLYMERASE GENE
    Donald Coen; Fiscal Year: 1993
    ..These studies should permit a detailed picture of the regulation and functional domains of Pol both in vitro and in infected cells and may uncover novel features of polymerases and new strategies for antiviral drug development...
  6. DNA-PROTEIN COMPLEX OF BACILLUS SUBTILIS PHAGE 029
    Margarita Salas; Fiscal Year: 1991
    ..There are indications that replication of poliovirus and encephalomyocarditis virus is initiated by a similar mechanism. The long term objective of the project will be to find specific ways to interfere with this new initiation reaction...
  7. p53 in Cellular Response to ROS-Mediated DNA Damage
    Peng Huang; Fiscal Year: 2007
    ..It is anticipated that the proposed studies will further our understanding of the mechanisms by which p53 affect cellular response to oxidative DNA damage and sensitivity to anticancer agents that generate ROS. ..
  8. CRYSTALLOGRAPHIC STUDIES OF DNA REPLICATION PROTEINS
    Tom Ellenberger; Fiscal Year: 2000
    ..5 protein. This entire project represents a long-term collaboration with Prof. Charles Richardson, also of Harvard. ..
  9. DNA REPAIR MECHANISMS IN USTILAGO MAYDIS
    William Holloman; Fiscal Year: 1999
    ..The aim is to identify likely interactors and to isolate mutants in these proteins by the usual reverse genetics approaches. It is anticipated that this may identify other genes that play a role in REC1-dependent activities. ..
  10. P53 AND REPAIR OF DNA DAMAGE BY ANTICANCER DRUGS
    Peng Huang; Fiscal Year: 2002
    ..Furthermore, the inability of mutant p53 to remove nucleoside analogs from DNA may provide a molecular basis for the design of strategies to selectively kill cancer cells with mutant p53 by therapeutic nucleoside analogs. ..
  11. DNA-PROTEIN COMPLEX OF BACILLUS SUBTILIS PHAGE 029
    Margarita Salas; Fiscal Year: 1993
    ..Evidence for a protein-primed mechanism of replication has been obtained for phi29, adenovirus and hepatitis B. The long term objective of the project is to find specific ways to interfere with this initiation reaction...
  12. Involvement of APC in DNA Repair
    Satya Narayan; Fiscal Year: 2006
    ..The findings of these studies will lay the basis for the development of a new class of chemotherapeutic agents for the prevention of colorectal cancers. ..
  13. STRUCTURE AND FUNCTION OF E COLI POLA GENE
    NIGEL GRINDLEY; Fiscal Year: 1993
    ..To facilitate the crystallographic studies, particularly those of Klenow fragment complexes with duplex DNA, we shall construct appropriately engineered derivatives of Klenow fragment...
  14. MOLECULAR GENETICS OF HSV DNA POLYMERASE GENE
    Donald Coen; Fiscal Year: 2009
    ..A combination of genetic, virological, biochemical, and crystallographic approaches will be initiated. Efforts to discover new agents that exploit this interaction (and to understand their mechanisms) will also begin. ..
  15. STRUCTURE AND FUNCTION OF E COLI POLA GENE
    NIGEL GRINDLEY; Fiscal Year: 1990
    ....
  16. DYNAMIC FLUORESCENCE STUDIES OF DNA-PROTEIN COMPLEXES
    David Millar; Fiscal Year: 2001
    ..The information obtained from this study will provide insight into the molecular mechanisms used to suppress mutations during DNA replication. ..
  17. Chemosensitization of Pancreatic Tumors via Inhibition of a DNA BER Enzyme, Ape1
    Melissa Fishel; Fiscal Year: 2007
    ....
  18. Discovering TREX1 and TREX2 Function in Mammalian Cells and Mice
    EDWARD HASTY; Fiscal Year: 2007
    ..Completion of this proposal will greatly facilitate our understanding of TREX1 and TREX2 for maintaining genome stability and for preventing cancer. ..
  19. REPAIR OF DNA DAMAGED BY UV IRRADIATION IN YEAST
    Louise Prakash; Fiscal Year: 2003
    ..The DNA replication/damage bypass proteins which become ubiquitinated in response to DNA damage will be identified, and the role of RAD6 and RAD18 in this process will be determined. ..
  20. Molecular Mechanisms of Metastasis Suppression by NM23
    DAVID KAETZEL; Fiscal Year: 2009
    ....
  21. Molecular Mechanisms of Metastasis Suppression by NM23
    DAVID KAETZEL; Fiscal Year: 2007
    ....
  22. FIDELITY OF DNA SYNTHESIS IN HEMATOPOETIC TISSUES
    ANTERO SO; Fiscal Year: 1993
    ..We plan to clone and sequence the cDNA for delta helicase, to analyze the structure and function of the enzyme, and to develop antibody and oligonucleotide reagents to elucidate the physiological role of this helicase...
  23. INITIATION OF MRNA DECAY IN BACILLUS SUBTILIS
    DAVID BECHHOFER; Fiscal Year: 2000
    ..Other endoribonuclease activities are likely to be present in B. Subtilis, and an in vitro system will be set up to detect such activities. ..