alkaline phosphatase


Summary: An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC

Top Publications

  1. pmc Comparative enzymology in the alkaline phosphatase superfamily to determine the catalytic role of an active-site metal ion
    Jesse G Zalatan
    Department of Chemistry, Stanford University, Beckman Center B400, Stanford, CA 94305, USA
    J Mol Biol 384:1174-89. 2008
  2. ncbi Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro
    N Jaiswal
    Osiris Therapeutics, Inc, Baltimore, MD 21231 2001
    J Cell Biochem 64:295-312. 1997
  3. doi In vitro osteogenesis assays: influence of the primary cell source on alkaline phosphatase activity and mineralization
    C D Hoemann
    Department of Chemical Engineering, Ecole Polytechnique, Montreal, QC, H3C 3A7, Canada
    Pathol Biol (Paris) 57:318-23. 2009
  4. ncbi Inhibitor of DNA binding/differentiation helix-loop-helix proteins mediate bone morphogenetic protein-induced osteoblast differentiation of mesenchymal stem cells
    Ying Peng
    Molecular Oncology Laboratory, Department of Surgery, and Committe on Genetics, University of Chicago, IL 60637, USA
    J Biol Chem 279:32941-9. 2004
  5. pmc Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota
    Jennifer M Bates
    Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA
    Cell Host Microbe 2:371-82. 2007
  6. pmc Active site detection by spatial conformity and electrostatic analysis--unravelling a proteolytic function in shrimp alkaline phosphatase
    Sandeep Chakraborty
    Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India
    PLoS ONE 6:e28470. 2011
  7. pmc An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily
    Bert van Loo
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom
    Proc Natl Acad Sci U S A 107:2740-5. 2010
  8. ncbi Connective tissue growth factor (CTGF) is regulated by Wnt and bone morphogenetic proteins signaling in osteoblast differentiation of mesenchymal stem cells
    Qing Luo
    Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, 5841 South Maryland Avenue, Chicago, IL 60637, USA
    J Biol Chem 279:55958-68. 2004
  9. ncbi Human circulating CD14+ monocytes as a source of progenitors that exhibit mesenchymal cell differentiation
    Masataka Kuwana
    Instiute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan
    J Leukoc Biol 74:833-45. 2003
  10. pmc Accelerated fat absorption in intestinal alkaline phosphatase knockout mice
    Sonoko Narisawa
    The Burnham Institute, La Jolla, California 92037, USA
    Mol Cell Biol 23:7525-30. 2003

Detail Information

Publications433 found, 100 shown here

  1. pmc Comparative enzymology in the alkaline phosphatase superfamily to determine the catalytic role of an active-site metal ion
    Jesse G Zalatan
    Department of Chemistry, Stanford University, Beckman Center B400, Stanford, CA 94305, USA
    J Mol Biol 384:1174-89. 2008
    ..In this study, we applied an alternative approach to the catalytic mechanism of alkaline phosphatase (AP), a widely studied prototypical bimetallo enzyme...
  2. ncbi Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro
    N Jaiswal
    Osiris Therapeutics, Inc, Baltimore, MD 21231 2001
    J Cell Biochem 64:295-312. 1997
    ..Optimal osteogenic differentiation, as determined by osteoblastic morphology, expression of alkaline phosphatase (APase), reactivity with anti-osteogenic cell surface monoclonal antibodies, modulation of osteocalcin mRNA ..
  3. doi In vitro osteogenesis assays: influence of the primary cell source on alkaline phosphatase activity and mineralization
    C D Hoemann
    Department of Chemical Engineering, Ecole Polytechnique, Montreal, QC, H3C 3A7, Canada
    Pathol Biol (Paris) 57:318-23. 2009
    ..With time in culture, cell differentiation is typically assessed by examining levels of alkaline phosphatase activity (an early osteoblast marker) and by evaluating the assembly of a collagen (type I)-containing ..
  4. ncbi Inhibitor of DNA binding/differentiation helix-loop-helix proteins mediate bone morphogenetic protein-induced osteoblast differentiation of mesenchymal stem cells
    Ying Peng
    Molecular Oncology Laboratory, Department of Surgery, and Committe on Genetics, University of Chicago, IL 60637, USA
    J Biol Chem 279:32941-9. 2004
  5. pmc Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota
    Jennifer M Bates
    Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA
    Cell Host Microbe 2:371-82. 2007
    ..Here, we show that expression of the zebrafish intestinal alkaline phosphatase (Iap), localized to the intestinal lumen brush border, is induced during establishment of the gut ..
  6. pmc Active site detection by spatial conformity and electrostatic analysis--unravelling a proteolytic function in shrimp alkaline phosphatase
    Sandeep Chakraborty
    Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India
    PLoS ONE 6:e28470. 2011
    ..Such a search has led us to predict a hitherto unknown function of shrimp alkaline phosphatase (SAP), where the protein acts as a protease...
  7. pmc An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily
    Bert van Loo
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom
    Proc Natl Acad Sci U S A 107:2740-5. 2010
    ..The crystal structure of PMH identifies it as a member of the alkaline phosphatase superfamily...
  8. ncbi Connective tissue growth factor (CTGF) is regulated by Wnt and bone morphogenetic proteins signaling in osteoblast differentiation of mesenchymal stem cells
    Qing Luo
    Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, 5841 South Maryland Avenue, Chicago, IL 60637, USA
    J Biol Chem 279:55958-68. 2004
  9. ncbi Human circulating CD14+ monocytes as a source of progenitors that exhibit mesenchymal cell differentiation
    Masataka Kuwana
    Instiute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan
    J Leukoc Biol 74:833-45. 2003
    ..Our findings represent the first evidence that human circulating CD14+ monocytes are a source of progenitors that exhibit mesenchymal cell differentiation...
  10. pmc Accelerated fat absorption in intestinal alkaline phosphatase knockout mice
    Sonoko Narisawa
    The Burnham Institute, La Jolla, California 92037, USA
    Mol Cell Biol 23:7525-30. 2003
    Intestinal alkaline phosphatase (IAP) is the most ancestral of the tissue-specific members of the AP gene family. Several studies have suggested an absorptive function for IAP, but in vivo data to this effect have been lacking...
  11. pmc Concerted regulation of inorganic pyrophosphate and osteopontin by akp2, enpp1, and ank: an integrated model of the pathogenesis of mineralization disorders
    Dympna Harmey
    The Burnham Institute, La Jolla, CA 92037, USA
    Am J Pathol 164:1199-209. 2004
    Tissue-nonspecific alkaline phosphatase (TNAP) hydrolyzes the mineralization inhibitor inorganic pyrophosphate (PP(i))...
  12. pmc Cellular function and molecular structure of ecto-nucleotidases
    Herbert Zimmermann
    Institute of Cell Biology and Neuroscience, Molecular and Cellular Neurobiology, Biologicum, Goethe University Frankfurt, Max von Laue Str 13, 60438, Frankfurt am Main, Germany
    Purinergic Signal 8:437-502. 2012
    ..The review focuses on the principal biochemical, cell biological, catalytic, and structural properties of the enzymes and provides brief reference to tissue distribution, and physiological and pathophysiological functions...
  13. pmc Roles of bone morphogenetic protein type I receptors and Smad proteins in osteoblast and chondroblast differentiation
    M Fujii
    Department of Biochemistry, The Cancer Institute of the Japanese Foundation for Cancer Research and Research for the Future Program, Tokyo, Japan
    Mol Biol Cell 10:3801-13. 1999
    ..BMPR-IB; BMPR-I group) and those of activin receptor-like kinase (ALK)-1 and ALK-2 (ALK-1 group) induced alkaline phosphatase activity in C2C12 cells...
  14. ncbi Gingival crevicular fluid alkaline phosphatase activity reflects periodontal healing/recurrent inflammation phases in chronic periodontitis patients
    Giuseppe Perinetti
    Department of Oral Sciences, University G D Annunzio, Chieti, Italy
    J Periodontol 79:1200-7. 2008
    ..One of these host enzymes is alkaline phosphatase (ALP), the GCF activity of which has been associated with periodontal inflammation...
  15. doi Physiological role of alkaline phosphatase explored in hypophosphatasia
    Michael P Whyte
    Shriners Hospital for Children, St Louis, Missouri, USA
    Ann N Y Acad Sci 1192:190-200. 2010 loss-of-function mutation(s) within TNSALP, the gene that encodes the "tissue nonspecific" isoenzyme of alkaline phosphatase (TNSALP). HPP reveals a critical role for this enzyme in skeletal mineralization...
  16. ncbi Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs)
    Hongwei Cheng
    Department of Surgery, The University of Chicago Medical Center, Illinois, USA
    J Bone Joint Surg Am 85:1544-52. 2003
    ..The optimal BMPs to be used in different clinical applications have not been elucidated, and a comprehensive evaluation of the relative osteogenic activity of different BMPs is lacking...
  17. ncbi Wnt signaling inhibits osteogenic differentiation of human mesenchymal stem cells
    Jan de Boer
    Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands
    Bone 34:818-26. 2004
    ..that both lithium and Wnt3A strongly inhibit dexamethasone-induced expression of the osteogenic marker alkaline phosphatase (ALP). Moreover, lithium partly inhibited mineralization of hMSCs whereas Wnt3A completely blocked it...
  18. ncbi Melatonin enhances alkaline phosphatase activity in differentiating human adult mesenchymal stem cells grown in osteogenic medium via MT2 melatonin receptors and the MEK/ERK (1/2) signaling cascade
    Nicholas M Radio
    Division of Pharmaceutical Sciences, Duquesne University, Pittsburgh, PA 15282, USA
    J Pineal Res 40:332-42. 2006
    ..human adult mesenchymal stem cell (hAMSC) differentiation into osteoblasts as assessed by measuring alkaline phosphatase (ALP) enzyme activity, and (b) identify potential signal transduction pathways that mediate this process...
  19. pmc Osteocyte control of bone formation via sclerostin, a novel BMP antagonist
    David G Winkler
    Department of Gene Function and Target Validation, Celltech R and D, Inc, Bothell, WA 98021, USA
    EMBO J 22:6267-76. 2003
    ..Modulation of this osteocyte-derived negative signal is therapeutically relevant for disorders associated with bone loss...
  20. ncbi Transcriptional characterization of bone morphogenetic proteins (BMPs)-mediated osteogenic signaling
    Ying Peng
    Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, Illinois 60637, USA
    J Cell Biochem 90:1149-65. 2003
    ..Further functional characterization of these downstream target genes should provide important insights into the molecular mechanisms behind BMP-mediated bone formation...
  21. doi Intestinal alkaline phosphatase: multiple biological roles in maintenance of intestinal homeostasis and modulation by diet
    Jean Paul Lallès
    Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1079 at Systèmes d Elevage, Nutrition Animale et Humaine in Saint Gilles, France
    Nutr Rev 68:323-32. 2010
    The diverse nature of intestinal alkaline phosphatase (IAP) functions has remained elusive, and it is only recently that four additional major functions of IAP have been revealed...
  22. ncbi Alkaline phosphatases reduce toxicity of lipopolysaccharides in vivo and in vitro through dephosphorylation
    Iwao Koyama
    Department of Medical Technology, Junior College, Saitama Medical School, 38 Morohongo, Moroyama, Iruma gun, Saitama 350 0495, Japan
    Clin Biochem 35:455-61. 2002
    Intestinal alkaline phosphatase (AP), as a host defense factor, was first investigated in vivo using rats orally exposed to lipopolysaccharide (LPS)...
  23. pmc Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product
    Y Ishino
    Department of Experimental Chemotherapy, Osaka University, Japan
    J Bacteriol 169:5429-33. 1987
    The iap gene in Escherichia coli is responsible for the isozyme conversion of alkaline phosphatase. We analyzed the 1,664-nucleotide sequence of a chromosomal DNA segment that contained the iap gene and its flanking regions...
  24. doi Role of alkaline phosphatase in colitis in man and rats
    A Tuin
    Department of Pharmacokinetics and Drug Delivery, University Centre for Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands
    Gut 58:379-87. 2009
    ..It has previously been shown that the enzyme alkaline phosphatase (AP) is able to detoxify LPS, and the aim of this study was to examine a possible role in IBDs.
  25. ncbi Effects of genistein and hormone-replacement therapy on bone loss in early postmenopausal women: a randomized double-blind placebo-controlled study
    Nunziata Morabito
    Department of Internal Medicine, University of Messina, Italy
    J Bone Miner Res 17:1904-12. 2002
  26. pmc BMP2 protein regulates osteocalcin expression via Runx2-mediated Atf6 gene transcription
    Won Gu Jang
    Dental Science Research Institute and BK21, School of Dentistry, Chonnam National University, Gwangju 500 757, Republic of Korea
    J Biol Chem 287:905-15. 2012
    ..BMP2 treatment was shown to markedly induce the expression and activation of ATF6 with an increase in alkaline phosphatase (ALP) and OC expression in MC3T3E1 cells...
  27. pmc Impaired calcification around matrix vesicles of growth plate and bone in alkaline phosphatase-deficient mice
    H Clarke Anderson
    Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Am J Pathol 164:841-7. 2004
    The presence of skeletal hypomineralization was confirmed in mice lacking the gene for bone alkaline phosphatase, ie, the tissue-non-specific isozyme of alkaline phosphatase (TNAP)...
  28. pmc Shear stress induces osteogenic differentiation of human mesenchymal stem cells
    Gregory Yourek
    Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL 60612, USA
    Regen Med 5:713-24. 2010
    ..To determine whether fluid flow-induced shear stress affects the differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) into osteogenic cells...
  29. ncbi Pyrophosphate inhibits mineralization of osteoblast cultures by binding to mineral, up-regulating osteopontin, and inhibiting alkaline phosphatase activity
    William N Addison
    Faculty of Dentistry, McGill University, Montreal, Quebec H3A 2B2, Canada
    J Biol Chem 282:15872-83. 2007
    ..tissues from mineralizing, whereas its degradation to P(i) in bones and teeth by tissue-nonspecific alkaline phosphatase (Tnap, Tnsalp, Alpl, Akp2) may facilitate crystal growth...
  30. pmc Enzyme replacement therapy for murine hypophosphatasia
    Jose Luis Millan
    Burnham Institute for Medical Research, La Jolla, California 92037, USA
    J Bone Miner Res 23:777-87. 2008
    ..caused by loss-of-function mutation(s) within the gene that encodes the tissue-nonspecific isozyme of alkaline phosphatase (TNALP)...
  31. ncbi Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/polyamide composite scaffolds for bone tissue engineering
    Huanan Wang
    Research Center for Nano Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, 610064, PR China
    Biomaterials 28:3338-48. 2007
    ..proliferation and differentiation of MSCs into osteoblastic phenotype were determined using MTT assay, alkaline phosphatase (ALP) activity and collagen type I (COL I) immunohistochemical staining and scanning electronic microscopy (..
  32. ncbi Evaluation of alkaline phosphatase immunoassay and comparison with other diagnostic methods in areas of low transmission of schistosomiasis
    B A Alarcón de Noya
    Seccion de Biohelmintiasis, Universidad Central de Venezuela, Caracas, Venezuela
    Acta Trop 66:69-78. 1997
    The alkaline phosphatase immunoassay (APIA) is an antibody detection technique which permits the diagnosis of schistosomiasis using a butanolic extract preparation from adult worms...
  33. ncbi Ecto 5'-nucleotidase and nonspecific alkaline phosphatase. Two AMP-hydrolyzing ectoenzymes with distinct roles in human airways
    Maryse Picher
    Cystic Fibrosis Pulmonary Research and Treatment Center, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 278:13468-79. 2003
    ..that mediated the conversion of AMP to adenosine: ecto 5'-nucleotidase (ecto 5'-NT, CD73) and alkaline phosphatase (AP)...
  34. pmc Alkaline phosphatase knock-out mice recapitulate the metabolic and skeletal defects of infantile hypophosphatasia
    K N Fedde
    Washington University School of Medicine at Barnes Jewish Hospital, St Louis, Missouri, USA
    J Bone Miner Res 14:2015-26. 1999 an inborn error of metabolism characterized by deficient activity of the tissue-nonspecific isoenzyme of alkaline phosphatase (TNSALP) and skeletal disease due to impaired mineralization of cartilage and bone matrix...
  35. ncbi Simultaneous quantitative analysis of sphingoid base 1-phosphates in biological samples by o-phthalaldehyde precolumn derivatization after dephosphorylation with alkaline phosphatase
    Jung Kee Min
    College of Pharmacy, Chungbuk National University, Chongju 361 763, South Korea
    Anal Biochem 303:167-75. 2002
    ..of two steps of sample pretreatment: the enzymatic dephosphorylation of sphingoid base 1-phosphates by alkaline phosphatase (APase) and the subsequent analysis of o-phthalaldehyde (OPA) derivatives of the liberated sphingoid bases ..
  36. ncbi Identification of novel Bacillus thuringiensis Cry1Ac binding proteins in Manduca sexta midgut through proteomic analysis
    Rebecca J McNall
    Department of Biochemistry and Molecular Biology, University of Georgia, 413 Biological Sciences Building, 120 Cedar Street, Athens, GA 30602, USA
    Insect Biochem Mol Biol 33:999-1010. 2003
    ..sexta proteins, but did match proteins of other Lepidoptera. Actin and alkaline phosphatase were identified as novel proteins that bind Cry1Ac in addition to the previously reported aminopeptidase N...
  37. pmc Distinct roles of transcription factors brn3a and brn3b in controlling the development, morphology, and function of retinal ganglion cells
    Tudor C Badea
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Neuron 61:852-64. 2009
    ..These findings reveal distinct roles for Brn3a and Brn3b in programming RGC diversity, and they illustrate the broad utility of germline methods for genetically manipulating and visualizing individual identified mammalian neurons...
  38. ncbi The mechanism of mineralization and the role of alkaline phosphatase in health and disease
    Hideo Orimo
    Division of Medical and Biological Chemistry, Graduate School of Medicine, Nippon Medical School, Bunkyo ku, Tokyo, Japan
    J Nippon Med Sch 77:4-12. 2010
    ..Tissue-nonspecific alkaline phosphatase (TNAP) hydrolyzes pyrophosphate and provides inorganic phosphate to promote mineralization...
  39. doi Enslavement in the water body by toxic Aphanizomenon ovalisporum, inducing alkaline phosphatase in phytoplanktons
    Yehonathan Bar-Yosef
    Department of Plant and Environmental Sciences, Edmond J Safra Campus, Givat Ram, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Curr Biol 20:1557-61. 2010
    ..Strong correlation between Aphanizomenon abundance in Lake Kinneret, Israel, and alkaline phosphatase (APase) activity suggests that inorganic phosphate (Pi) limitation induces the PHO regulon and APase ..
  40. doi Impact of growth factors and PTHrP on early and late chondrogenic differentiation of human mesenchymal stem cells
    S Weiss
    Division of Experimental Orthopaedics, Orthopaedic University Hospital Heidelberg, Heidelberg, Germany
    J Cell Physiol 223:84-93. 2010
    ..Differentiation was assessed by immunohistochemistry, alcian-blue staining, qRT-PCR, and quantification of alkaline phosphatase (ALP) activity...
  41. ncbi Bone turnover markers as predictors of skeletal complications in prostate cancer, lung cancer, and other solid tumors
    Janet E Brown
    Academic Unit of Clinical Oncology, Cancer Research Centre, Weston Park Hospital, Sheffield, England, United Kingdom
    J Natl Cancer Inst 97:59-69. 2005
    ..We investigated this question in patients with bone metastases secondary to prostate cancer and to non-small-cell lung cancer (NSCLC) and other solid tumors assigned to the placebo arms of two phase III trials of zoledronic acid...
  42. pmc Association of Cry1Ac toxin resistance in Helicoverpa zea (Boddie) with increased alkaline phosphatase levels in the midgut lumen
    Silvia Caccia
    Department of Genetics, University of Valencia, Burjassot Valencia, Spain
    Appl Environ Microbiol 78:5690-8. 2012
    ..Enzymatic activities of two putative Cry1Ac receptors (aminopeptidase N [APN] and alkaline phosphatase [ALP]) were significantly reduced (2-fold and 3-fold, respectively) in BBMVs from AR1 compared to LC larvae...
  43. pmc Mechanism for release of alkaline phosphatase caused by glycosylphosphatidylinositol deficiency in patients with hyperphosphatasia mental retardation syndrome
    Yoshiko Murakami
    Department of Immunoregulation, Research Institute for Microbial Diseases, and Laboratory of Immunoglycobiology, WPI Immunology Frontier Research Center, Osaka, Japan 565 0871, Japan
    J Biol Chem 287:6318-25. 2012
    ..syndrome (HPMR), an autosomal recessive disease characterized by mental retardation and elevated serum alkaline phosphatase (ALP) levels, is caused by mutations in the coding region of the phosphatidylinositol glycan anchor ..
  44. pmc Serum alkaline phosphatase and phosphate and risk of mortality and hospitalization
    Matthew Abramowitz
    Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York, USA
    Clin J Am Soc Nephrol 5:1064-71. 2010
    Elevated alkaline phosphatase (AlkPhos) and phosphate levels are associated with cardiovascular morbidity and mortality in patients receiving dialysis...
  45. doi Association between serum alkaline phosphatase and C-reactive protein in the United States National Health and Nutrition Examination Survey 2005-2006
    Matthew Webber
    School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK
    Clin Chem Lab Med 48:167-73. 2010
    b>Alkaline phosphatase (ALP) is a widely used marker for skeletal and hepatobiliary disorders, but its activity is also increased in atherosclerosis and peripheral vascular disease. It is an inflammatory marker like C-reactive protein (CRP)...
  46. doi Matrix gla protein and alkaline phosphatase are differently modulated in human dermal fibroblasts from PXE patients and controls
    Federica Boraldi
    Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy
    J Invest Dermatol 133:946-54. 2013 standard conditions and in calcifying medium (CM) in the presence of vit K1 and K2, or levamisole, an alkaline phosphatase (ALP) inhibitor...
  47. pmc Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering
    Peiyan Ni
    State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan, People s Republic of China
    Int J Nanomedicine 6:3065-75. 2011
    ..The significantly higher alkaline phosphatase activity of the PEG/PLA fibrous scaffolds indicated that these can enhance the differentiation of MSCs into ..
  48. ncbi Structural evidence of functional divergence in human alkaline phosphatases
    Marie Helene Le Du
    Département d Ingénierie et d Etudes des Protéines DIEP, CEA, Bat 152 C E Saclay, 91191 Gif sur Yvette Cedex, France
    J Biol Chem 277:49808-14. 2002
    The evolution of the alkaline phosphatase (AP) gene family has lead to the existence in humans of one tissue-nonspecific (TNAP) and three tissue-specific isozymes, i.e. intestinal (IAP), germ cell (GCAP), and placental AP (PLAP)...
  49. pmc Modeling catalytic promiscuity in the alkaline phosphatase superfamily
    Fernanda Duarte
    Uppsala University, Science for Life Laboratory SciLifeLab, Cell and Molecular Biology, Uppsala, Sweden
    Phys Chem Chem Phys 15:11160-77. 2013
    ..As a case study, we will discuss recent computational work on different members of the alkaline phosphatase superfamily (AP) using a range of different approaches, showing the complementary insights they have ..
  50. ncbi Hydrogen peroxide-induced oxidative stress in MC3T3-E1 cells: The effects of glutamate and protection by purines
    Amos A Fatokun
    Institute of Biomedical and Life Sciences, Division of Neuroscience and Biomedical Systems, West Medical Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Bone 39:542-51. 2006
    ..Adenosine and its receptors afforded some protection to osteoblasts against cellular death mediated partly by apoptosis and partly by necrosis...
  51. ncbi Novel inhibitors of alkaline phosphatase suppress vascular smooth muscle cell calcification
    Sonoko Narisawa
    The Burnham Institute, La Jolla, California, USA
    J Bone Miner Res 22:1700-10. 2007
    We report three novel inhibitors of the physiological pyrophosphatase activity of alkaline phosphatase and show that these compounds are capable of reducing calcification in two models of vascular calcification (i.e...
  52. pmc Review of hair follicle dermal cells
    Chao Chun Yang
    Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Dermatol Sci 57:2-11. 2010
  53. ncbi Alkaline phosphatase activity in gingival crevicular fluid during canine retraction
    P Batra
    Centre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi, India
    Orthod Craniofac Res 9:44-51. 2006
    The aim of the study was to investigate alkaline phosphatase activity in the gingival crevicular fluid (GCF) during orthodontic tooth movement in humans.
  54. ncbi Characterization of adhesion and differentiation markers of osteogenic marrow stromal cells
    R Marom
    Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Israel
    J Cell Physiol 202:41-8. 2005
    ..of extracellular matrix proteins that are a prerequisite for matrix formation and mineralization, including alkaline phosphatase (ALP), osteocalcin, osteopontin, biglycan, and bone sialoprotein (BSP)...
  55. pmc Disulfide bond formation in the Escherichia coli cytoplasm: an in vivo role reversal for the thioredoxins
    E J Stewart
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    EMBO J 17:5543-50. 1998
    ..This disulfide bond formation can be detected by assessing the state of the normally periplasmic enzyme alkaline phosphatase (AP) when it is localized to the cytoplasm...
  56. doi Biomarkers of potential harm among adult smokers and nonsmokers in the total exposure study
    Kimberly Frost-Pineda
    Altria Client Services, 601 East Jackson Street, Richmond, VA 23219, USA
    Nicotine Tob Res 13:182-93. 2011
    ..The BOPH included markers of oxidative stress, inflammation, platelet activation, endothelial function, lipid metabolism, hematology, metabolism, the cardiovascular system, lung function, kidney function, and liver function...
  57. doi TNF-alpha and IL-1beta inhibit RUNX2 and collagen expression but increase alkaline phosphatase activity and mineralization in human mesenchymal stem cells
    J Ding
    Laboratory of Cellular and Molecular Biology EA2603, IFR114, ULCO, Universite Lille Nord de France, France
    Life Sci 84:499-504. 2009
    ..Our aims were to clarify the effects of tumour necrosis factor alpha (TNF-alpha) and interleukin 1beta (IL-1beta) on osteoblast differentiation and mineralization in human mesenchymal stem cells (MSCs)...
  58. ncbi Metallothionein protects bone marrow stromal cells against hydrogen peroxide-induced inhibition of osteoblastic differentiation
    An Ling Liu
    Yingdong College of Biotechnology, Shaoguan University, Shaoguan, Guangdong Province 512005, PR China
    Cell Biol Int 28:905-11. 2004
    ..of primary mouse bone marrow stromal cells (BMSCs), manifested by a reduction in the differentiation marker alkaline phosphatase (ALP)...
  59. doi Inhibition of cathepsin K for treatment of osteoporosis
    Steven Boonen
    Leuven University Division of Geriatric Medicine and Centre for Metabolic Bone Diseases, UZ Leuven Campus Gasthuisberg, Herestraat 49, B 3000 Leuven, Belgium
    Curr Osteoporos Rep 10:73-9. 2012
    ..The usefulness of cathepsin K inhibitors in osteoporosis is now being examined in phase 2 and phase 3 clinical trials of postmenopausal osteoporotic women...
  60. pmc Serotonergic 5-HT(2B) receptor controls tissue-nonspecific alkaline phosphatase activity in osteoblasts via eicosanoids and phosphatidylinositol-specific phospholipase C
    Anne Baudry
    Cellules Souches, Signalisation et Prions, INSERM U747, Universite Paris Descartes, Paris, France
    J Biol Chem 285:26066-73. 2010
    ..A functional link between the 5-HT(2B)R and the activity of the tissue-nonspecific alkaline phosphatase (TNAP) is established using the C1 osteoprogenitor cell line...
  61. pmc In vitro reconstitution of PHO5 promoter chromatin remodeling points to a role for activator-nucleosome competition in vivo
    Franziska Ertel
    Adolf Butenandt Institut, University of Munich, Schillerstr 44, 80336 Munich, Germany
    Mol Cell Biol 30:4060-76. 2010
    ..These data establish an auxiliary role for DNA binding competition between Pho4 and histones in PHO5 promoter chromatin remodeling in vivo...
  62. doi Epidermal growth factor and bone morphogenetic proteins upregulate osteoblast proliferation and osteoblastic markers and inhibit bone nodule formation
    Claude Laflamme
    Groupe de Recherche en Ecologie Buccale, Faculte de Medecine Dentaire, Pavillon de Medecine dentaire, Universite Laval, Quebec, Canada G1K 7P4
    Arch Oral Biol 55:689-701. 2010
    ..The aim of this study was to investigate the in vitro osteogenic activity of EGF in association with bone morphogenetic proteins BMP2 and BMP7...
  63. pmc Wnt/beta-catenin inhibits dental pulp stem cell differentiation
    E L Scheller
    Laboratory of Molecular Signaling, Department of Biologic and Materials Sciences, School of Dentistry, The University of Michigan, Ann Arbor, MI 48109, USA
    J Dent Res 87:126-30. 2008
    ..Unexpectedly, Wnt-1 inhibited alkaline phosphatase (ALP) activity and the formation of mineralized nodules in DPSCs...
  64. pmc Evidence of associations between feto-maternal vitamin D status, cord parathyroid hormone and bone-specific alkaline phosphatase, and newborn whole body bone mineral content
    Daphna K Dror
    USDA, ARS, Western Human Nutrition Research Center, 430 W Health Sciences Dr, Davis, CA 95616, USA
    Nutrients 4:68-77. 2012
    ..and neonates, relationships among vitamin D status (25(OH)D), parathyroid hormone (PTH), bone specific alkaline phosphatase (BALP), and whole body bone mineral content (WBBMC) in the newborn are poorly characterized...
  65. pmc Tissue-nonspecific alkaline phosphatase acts redundantly with PAP and NT5E to generate adenosine in the dorsal spinal cord
    Sarah E Street
    Department of Cell Biology and Physiology, and Department of Chemistry, Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Neurosci 33:11314-22. 2013
    ..Here, we found that tissue-nonspecific alkaline phosphatase (TNAP, encoded by the Alpl gene) is expressed and functional in DRG neurons and spinal neurons...
  66. doi In vitro study of enhanced osteogenesis induced by HIF-1α-transduced bone marrow stem cells
    D Zou
    School of Stomatology, Tongji University, Shanghai, China
    Cell Prolif 44:234-43. 2011
    ..For better understanding of HIF-1α promoting osteogenesis, the role of HIF-1α-induced MSC osteogenesis in the normoxic state has been investigated here...
  67. pmc Tissue nonspecific alkaline phosphatase is activated via a two-step mechanism by zinc transport complexes in the early secretory pathway
    Ayako Fukunaka
    Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan
    J Biol Chem 286:16363-73. 2011
    ..ZnT1 and ZnT4 expressed in chicken DT40 cells did not contribute to the activation of tissue nonspecific alkaline phosphatase (TNAP)...
  68. pmc Adeno-associated virus serotype 6 capsid tyrosine-to-phenylalanine mutations improve gene transfer to skeletal muscle
    Chunping Qiao
    Division of Molecular Pharmaceutics, University of North Carolina Eshelman School of Pharmacy, Chapel Hill, NC 27599, USA
    Hum Gene Ther 21:1343-8. 2010
    ..Expression of both firefly luciferase and alkaline phosphatase reporter genes increased up to 8-fold and DNA copy numbers in muscle increased up to 6-fold...
  69. pmc Evaluation of calcium acetate/magnesium carbonate as a phosphate binder compared with sevelamer hydrochloride in haemodialysis patients: a controlled randomized study (CALMAG study) assessing efficacy and tolerability
    Angel L M de Francisco
    Hospital Marques de Valdecilla, Universidad de Cantabria, Santander, Spain
    Nephrol Dial Transplant 25:3707-17. 2010
    ..Phosphate binders are required to control serum phosphorus in dialysis patients. A phosphate binder combining calcium and magnesium offers an interesting therapeutic option...
  70. doi Treatment with hydrogen molecule alleviates TNFα-induced cell injury in osteoblast
    Wen Wen Cai
    The Centre of Drug Safeguard, Chinese People s Liberation Army General Hospital, Beijing, People s Republic of China
    Mol Cell Biochem 373:1-9. 2013
    ..TNFα suppressed cell viability, induced cell apoptosis, suppressed Runx2 mRNA expression, and inhibited alkaline phosphatase activity, which was reversed by co-incubation with H(2)...
  71. doi Schistosoma mansoni: molecular characterization of Alkaline Phosphatase and expression patterns across life cycle stages
    B O Araujo-Montoya
    Centro de Biotecnologia, Instituto Butantan, Av Vital Brazil 1500, Sao Paulo, SP, Brazil
    Exp Parasitol 129:284-91. 2011
    Here we describe the cloning and characterization of the Schistosoma mansoni Alkaline Phosphatase (SmAP), previously identified in the tegument of adult worms...
  72. pmc Valproic acid induces hair regeneration in murine model and activates alkaline phosphatase activity in human dermal papilla cells
    Soung Hoon Lee
    Translational Research Center for Protein Function Control, Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea
    PLoS ONE 7:e34152. 2012
    ..We have identified a new function for valproic acid (VPA), a GSK3β inhibitor that activates the Wnt/β-catenin pathway, to promote hair re-growth in vitro and in vivo...
  73. pmc Intestinal alkaline phosphatase prevents metabolic syndrome in mice
    Kanakaraju Kaliannan
    Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 110:7003-8. 2013
    ..In the present study we examined the role of the small intestinal brush-border enzyme, intestinal alkaline phosphatase (IAP), in preventing a high-fat-diet-induced metabolic syndrome in mice...
  74. pmc Multiple receptors as targets of Cry toxins in mosquitoes
    Supaporn Likitvivatanavong
    Department of Cell Biology and Neuroscience, University of California, Riverside, California 92521, United States
    J Agric Food Chem 59:2829-38. 2011
    ..toxin and multiple receptors in mosquitoes, in particular Aedes aegypti and reviews the manner by which the receptors were identified and characterized, with a focus on three proteins, cadherin, alkaline phosphatase, and aminopeptidase-N.
  75. doi When and how to evaluate mildly elevated liver enzymes in apparently healthy patients
    George Aragon
    Center for Liver Diseases, Inova Fairfax Hospital, Falls Church, VA 22042, USA
    Cleve Clin J Med 77:195-204. 2010
    ..This review discusses likely causes of elevated aminotransferase, alkaline phosphatase, and gamma-glutamyl transferase levels and provides algorithms for evaluating high liver enzyme values in ..
  76. pmc Resolvin E1-induced intestinal alkaline phosphatase promotes resolution of inflammation through LPS detoxification
    Eric L Campbell
    Mucosal Inflammation Program, Department of Medicine, University of Colorado, Aurora, CO 80045, USA
    Proc Natl Acad Sci U S A 107:14298-303. 2010
    ..Notably, RvE1 induced intestinal alkaline phosphatase (ALPI) expression and significantly enhanced epithelial ALPI enzyme activity...
  77. ncbi Ghrelin directly regulates bone formation
    Nobuhiro Fukushima
    Molecular Genetics, Institute of Life Science, Kurume University, Kurume, Fukuoka, Japan
    J Bone Miner Res 20:790-8. 2005
    ..Ghrelin and its receptor, GHS-R1a, were identified in osteoblasts, and ghrelin promoted both proliferation and differentiation. Furthermore, ghrelin increased BMD in rats. Our results show that ghrelin directly affects bone formation...
  78. doi Epigenetic regulation of alkaline phosphatase in human cells of the osteoblastic lineage
    Jesús Delgado-Calle
    Department of Internal Medicine, Hospital U M Valdecilla IFIMAV, University of Cantabria, Santander, Spain
    Bone 49:830-8. 2011
    ..This study analyzed the relationship between tissue non-specific alkaline phosphatase (ALPL) gene expression and the methylation of a CpG island located in its proximal region...
  79. doi Selection of alkaline phosphatase-positive induced pluripotent stem cells from human amniotic fluid-derived cells by feeder-free system
    Huai En Lu
    Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan, ROC
    Exp Cell Res 317:1895-903. 2011
    ..In this study, we report a simplistic, one-step method for selection of AP+ AF-iPS cells via feeder-free screening...
  80. pmc Identification of specific targets for the gut mucosal defense factor intestinal alkaline phosphatase
    Kathryn T Chen
    Dept of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Am J Physiol Gastrointest Liver Physiol 299:G467-75. 2010
    Intestinal alkaline phosphatase (IAP) is a small intestinal brush border enzyme that has been shown to function as a gut mucosal defense factor, but its precise mechanism of action remains unclear...
  81. doi Osteoblastogenesis and osteoprotection enhanced by flavonolignan silibinin in osteoblasts and osteoclasts
    Jung Lye Kim
    Department of Food and Nutrition, Hallym University, Chuncheon, Korea
    J Cell Biochem 113:247-59. 2012
    ..In addition, silibinin furthered the induction of osteoblastogenic biomarkers of alkaline phosphatase, collagen type 1, connective tissue growth factor, and bone morphogenetic protein-2...
  82. pmc Intestinal alkaline phosphatase has beneficial effects in mouse models of chronic colitis
    Sundaram Ramasamy
    Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Inflamm Bowel Dis 17:532-42. 2011
    The brush border enzyme intestinal alkaline phosphatase (IAP) functions as a gut mucosal defense factor and is protective against dextran sulfate sodium (DSS)-induced acute injury in rats...
  83. ncbi Comparison of capillary architecture between slow and fast muscles in rats using a confocal laser scanning microscope
    Shinichiro Murakami
    Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Acta Med Okayama 64:11-8. 2010
    ..To investigate capillary density, sections of both types of muscle were stained with alkaline phosphatase; the soleus muscle showed more intense reactivity, indicating that it had a denser capillary structure than ..
  84. ncbi Anti-inflammatory effects of alkaline phosphatase in coronary artery bypass surgery with cardiopulmonary bypass
    Suzanne Kats
    Catharina Hospital, Department of Cardiothoracic Surgery, Eindhoven, The Netherlands
    Recent Pat Inflamm Allergy Drug Discov 3:214-20. 2009
    ..b>Alkaline phosphatase prevents endotoxin-induced systemic inflammation in animals and humans...
  85. doi Intestinal alkaline phosphatase preserves the normal homeostasis of gut microbiota
    M S Malo
    Department of Surgery, Massachusetts General Hospital, Boston, MA 02114, USA
    Gut 59:1476-84. 2010
    ..Previously it was shown that intestinal alkaline phosphatase (IAP), a small intestinal brush border enzyme, functions as a gut mucosal defence factor limiting the ..
  86. ncbi Induction of bone-type alkaline phosphatase in human vascular smooth muscle cells: roles of tumor necrosis factor-alpha and oncostatin M derived from macrophages
    Atsushi Shioi
    Department of Cardiovascular Medicine, Osaka City University Graduate School of Medicine, Osaka, Japan
    Circ Res 91:9-16. 2002
    ..1alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3) on in vitro calcification through increasing the expression of alkaline phosphatase (ALP), an ectoenzyme indispensable for bone mineralization, in vascular smooth muscle cells...
  87. pmc Isolation, transcription, and inactivation of the gene for an atypical alkaline phosphatase of Synechococcus sp. strain PCC 7942
    J M Ray
    Department of Plant Biology, Carnegie Institution of Washington, Stanford, California 94305
    J Bacteriol 173:4297-309. 1991
    The alkaline phosphatase of Synechococcus sp. strain PCC 7942 is 145 kDa, which is larger than any alkaline phosphatase previously characterized and approximately three times the size of the analogous enzyme in Escherichia coli...
  88. ncbi Transforming growth factor-beta1 mechanisms in aortic valve calcification: increased alkaline phosphatase and related events
    Jocelyn N Clark-Greuel
    Division of Cardiology, The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104 4318, USA
    Ann Thorac Surg 83:946-53. 2007
    ..Previous investigations by our group have shown a strong association of transforming growth factor-beta1 (TGF-beta1)-related mechanisms with calcific aortic stenosis in both cell culture and clinical pathology studies...
  89. pmc Variability of parathyroid hormone and other markers of bone mineral metabolism in patients receiving hemodialysis
    Clare Gardham
    Department of Clinical Biochemistry, East Kent Hospitals University NHS Foundation Trust, Kent and Canterbury Hospital, Ethelbert Road, Canterbury, Kent CT1 3NG, United Kingdom
    Clin J Am Soc Nephrol 5:1261-7. 2010
    ..The biologic variation of PTH and other bone mineral markers was measured in hemodialysis patients to better define their role in management...
  90. ncbi Mice lacking tissue non-specific alkaline phosphatase die from seizures due to defective metabolism of vitamin B-6
    K G Waymire
    Department of Genetics and Molecular Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Nat Genet 11:45-51. 1995
    In humans, deficiency of the tissue non-specific alkaline phosphatase (TNAP) gene is associated with defective skeletal mineralization...
  91. doi Involvement of BMPs/Smad signaling pathway in mechanical response in osteoblasts
    Liang Wang
    Tianjin Institute of Medical Equipment, Tianjin, China
    Cell Physiol Biochem 26:1093-102. 2010
    ..The aim of this study was to determine whether Bone Morphogenetic Proteins (BMPs)/Smad signaling pathway is involved in mechanical response in osteoblasts...
  92. ncbi Inactivation of two mouse alkaline phosphatase genes and establishment of a model of infantile hypophosphatasia
    S Narisawa
    The Burnham Institute, La Jolla Cancer Research Center, California 92037, USA
    Dev Dyn 208:432-46. 1997
    We report the inactivation, via homologous recombination, of two of the three active mouse alkaline phosphatase genes, i.e., embryonic (EAP) and tissue nonspecific (TNAP)...
  93. ncbi Longitudinal monitoring of subgingival colonization by Actinobacillus actinomycetemcomitans, and crevicular alkaline phosphatase and aspartate aminotransferase activities around orthodontically treated teeth
    Giuseppe Perinetti
    Unit of Orthodontics, Department of Oral Sciences, School of Dentistry, University G D Annunzio, Chieti, Italy
    J Clin Periodontol 31:60-7. 2004
    ..a longitudinal design to examine subgingival colonization of Actinobacillus actinomycetemcomitans (Aa) and alkaline phosphatase (ALP) and aspartate aminotransferase (AST) activities in gingival crevicular fluid (GCF) in order to assess ..
  94. doi Oxygen tension differentially influences osteogenic differentiation of human adipose stem cells in 2D and 3D cultures
    Jiawei He
    Department of Biomedical Engineering, University of California, Davis, Davis, California 95616, USA
    J Cell Biochem 110:87-96. 2010
    ..We detected greatest alkaline phosphatase activity and extracellular calcium deposition in cells cultured in both 2D and 3D under 21% oxygen, and ..
  95. doi New bone formation by allogeneic mesenchymal stem cell transplantation in a patient with perinatal hypophosphatasia
    Mika Tadokoro
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Hyogo, Japan
    J Pediatr 154:924-30. 2009
    ..We demonstrated the importance of allogeneic MSC transplantation for hypophosphatasia and the constructs as an alternative to bone fragments that provided further osteogenic capability in the patient...
  96. doi The alkaline phosphatase PhoX is more widely distributed in marine bacteria than the classical PhoA
    Marta Sebastian
    Institute of Marine and Coastal Sciences, Rutgers University, New Brunswick, NJ 08901 8521, USA
    ISME J 3:563-72. 2009
    ..when inorganic phosphate (P(i)) is insufficient to meet their P-requirements, and therefore bulk alkaline phosphatase activity measurements have been used to assess the P-status of microbial assemblages...
  97. pmc Intestinal alkaline phosphatase is a gut mucosal defense factor maintained by enteral nutrition
    Ross F Goldberg
    Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 105:3551-6. 2008
    ..Using in vitro and in vivo models, we demonstrate that the brush-border enzyme, intestinal alkaline phosphatase (IAP), has the ability to detoxify lipopolysaccharide and prevent bacterial invasion across the gut mucosal ..
  98. ncbi Cultured peribulbar dermal sheath cells can induce hair follicle development and contribute to the dermal sheath and dermal papilla
    Kevin J McElwee
    Department of Dermatology, Philipp University, Marburg, Germany
    J Invest Dermatol 121:1267-75. 2003
    ..DSC cells were characterized in vivo and in vitro by low alkaline phosphatase activity in contrast to high alkaline phosphatase in DP cells...
  99. ncbi Changes in Runx2/Cbfa1 expression and activity during osteoblastic differentiation of human bone marrow stromal cells
    Chaoxiang Shui
    Endocrine Research Unit, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, USA
    J Bone Miner Res 18:213-21. 2003
    ..reverse transcription-polymerase chain reaction (RT-PCR) analysis demonstrated that the expression of alkaline phosphatase and osteocalcin mRNAs increased in a time-dependent manner with the development of the osteoblast phenotype ..
  100. pmc Melanopsin-expressing retinal ganglion-cell photoreceptors: cellular diversity and role in pattern vision
    Jennifer L Ecker
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
    Neuron 67:49-60. 2010
    ..Thus, nonclassical retinal photoreception occurs within diverse cell types and influences circuits and functions encompassing luminance as well as spatial information...
  101. doi Inorganic polymeric phosphate/polyphosphate as an inducer of alkaline phosphatase and a modulator of intracellular Ca2+ level in osteoblasts (SaOS-2 cells) in vitro
    Werner E G Muller
    Institute of Physiological Chemistry, University Medical Center of the Johannes Gutenberg University Mainz, Duesbergweg 6, D 55128 Mainz, Germany
    Acta Biomater 7:2661-71. 2011
    ..At a concentration of 100 μM, polyP (Ca(2+) salt) caused a strong increase in the activity of the alkaline phosphatase and also an induction of the steady-state expression of the gene encoding this enzyme...

Research Grants68

  1. Activators of the Pyrophosphatase Activity of Alkaline Phosphatase
    Jose Luis Millan; Fiscal Year: 2007
    ..We have recently reported that the rickets and osteomalacia characteristic in tissue-nonspecific alkaline phosphatase (TNAP)-deficient mice (Akp2-/- mice) results from highly increased levels of inorganic pyrophosphate (PP), ..
  2. Classical and non-classical responses to vitamin D in children: the role of DBP g
    Thomas O Carpenter; Fiscal Year: 2010
    ..of vitamin D on the bone and mineral system, with outcome measures to include: circulating levels of PTH, alkaline phosphatase activity, osteocalcin, P1NP, and FGF23 as markers of a vitamin D effect on the skeleton and bone mineral ..
  3. Modeling distinct neonatal purine metabolism to inform vaccine development
    Ofer Levy; Fiscal Year: 2013
    ..Neonatal plasma contains high concentrations of Ado-generating alkaline phosphatase (AP) and 5'ectonucleotidase CD73 while containing less Ado deaminase (ADA) than adult plasma, ..
  4. Functional characterization of ATP1A1 and DEspR variants associated with essentia
    Nelson Ruiz Opazo; Fiscal Year: 2010
    ..the rs6535847 T alleles (pTATAAAA) in tissue culture cells using SEAP (a secreted form of human placental alkaline phosphatase) as a reporter molecule to monitor activity...
  5. Leads and Target Validation for Vascular Calcification in Chronic Kidney Disease
    Jose Luis Millan; Fiscal Year: 2010
    ..The key regulator of extracellular PPi is the ectoenzyme tissue-nonspecific alkaline phosphatase (TNAP) that hydrolyzes PPi, thus destroying its ability to suppress calcification...
  6. Targeted delivery of osteoinductive molecules to biologic HA in bone grafts
    Jennifer L Bain; Fiscal Year: 2012
    ..Bone formation markers such as alkaline phosphatase and osteocalcin will be measured...
  7. DnaK Protein Inhibitor Antimicrobials
    Michael Sturgess; Fiscal Year: 2003
    ..the protein, and, if inconclusive data are obtained, the inhibition of protein folding as indicated by the alkaline phosphatase and beta-galactosidase activity of E. coli cultures. The ability of the peptide analogs to enter E...
  8. SPRINT - Factors Affecting Atherosclerosis Study (FAST)
    Srinivasan Beddhu; Fiscal Year: 2013
    ..obesity and factors reflective of abnormal bone and mineral metabolism (plasma calcium, phosphorus, alkaline phosphatase, 25 and 1,25 vitamin D and fibroblast growth factor 23 levels) play a significant role in progression of ..
  9. Molecular Pathogenesis and Treatment of Hypophosphatasia
    Jose Luis Millan; Fiscal Year: 2013
    ..that features rickets or osteomalacia due to loss-of-function mutation within the tissue-nonspecific alkaline phosphatase (TNAP) gene. To-date, there is no established medical treatment for this condition...
  10. Mineralization Gene Variants: Biochemical Implications and Associations with BMD
    Carrie M Nielson; Fiscal Year: 2013
    ..The research proposal focuses on characterizing alkaline phosphatase (ALPL) gene mutations for their effect on enzyme activity in vitro and on their associations with BMD in ..
  11. Wear Particle Disease and NF-kappa B Signaling
    Stuart B Goodman; Fiscal Year: 2013
    ..OPC cultures will be assayed for alkaline phosphatase and osteocalcin expression, and bone matrix formation will be quantitated using Von Kossa staining...
  12. Smad4 promotes intestinal homeostasis through suppression of Wnt signaling
    Tanner J Freeman; Fiscal Year: 2013
    ..Using tissues from our murine models, differentiation (intestinal alkaline phosphatase, muc2, chromogranin A, lysozyme) and stem cell marker expression (Lgr5, Lrig1, Ascl2, Olfm4, DcamKL1, Mshi1)..
  13. Flow Perfusion Bioreactor Fabrication of Bioactive Polymer/ECM Hybrid Constructs
    Antonios G Mikos; Fiscal Year: 2013
    ..differentiation of subsequently seeded MSCs in vitro (as measured by differentiation markers such as alkaline phosphatase activity, calcium and glycosaminoglycan content, and the presence of collage types I and II) and upon ..
  14. Elucidating the regulation of mitochondrial glutaminase in cancer progression
    Kelly E Sullivan; Fiscal Year: 2013
    ..Specifically, alkaline phosphatase treatment of GAC immunoprecipitated from transformed cells drastically reduces the basal activity of this ..
    Julie Glowacki; Fiscal Year: 2010
    ..bone marrow stromal cells (hMSCs) undergo an age-dependent decrease in capacity for differentiation to alkaline phosphatase-positive (AlkP+) cells...
  16. Contribution of altered muscle hemodynamics to fatigability in older persons with
    CHARLOTTE A contact PETERSON; Fiscal Year: 2010 muscle biopsies by immunofluorescent detection of endothelial cell antigens and detection of endothelial alkaline phosphatase, and non-invasively with the flow-mediated dilatation (FMD) test and optical probe...
  17. Role of TRPM4 in dental follicle stem cell differentiation
    Henrique Cheng; Fiscal Year: 2010
    ..TRPM4 knockdown clones will be cultured in osteoblast or adipocyte differentiation medium and examined for alkaline phosphatase enzyme activity and for the presence of Ca2+ deposition in the extracellular matrix using Alizarin Red S (..
  18. Evaluation of Recombinant Human Lactoferrin as an Osteogenic Biomaterial
    Ashley A Amini; Fiscal Year: 2013
    ..Additionally, osteocalcin, osteopontin and Runx2, alkaline phosphatase expression levels will be assessed to study the effects of the rhLF gel on cell differentiation...
    Richard A Hodin; Fiscal Year: 2010
    ..This proposal is based upon a novel hypothesis that the loss of intestinal alkaline phosphatase (IAP) expression underlies the impairment in gut mucosal defense that occurs in critically ill patients...
  20. Examination of the Pre-pubertal Mammalian Growth Plate
    ..patterns of genes known to be differentially expressed in histologic growth plate zones (collagen X, alkaline phosphatase, Indian hedgehog, and IGFBP5)...
  21. Injectable, In Situ Forming Hydrogels for Craniofacial Tissue Engineering
    Tiffany Vo; Fiscal Year: 2013
    ..and essential biochemical assays for cell proliferation, mineralized extracellular matrix production and alkaline phosphatase activity...
  22. Imaging Enzyme Structure and Dynamics: Specific Function and Functional Diversity
    Hua Deng; Fiscal Year: 2010
    ..Two enzyme systems, protein-tyrosine phosphatase (PTPase) and alkaline phosphatase (AP), will be studied...
  23. The mechanism of a Chinese herbal formula on cancer pain
    Ling Xu; Fiscal Year: 2012 that osteoclastic activitiesare involved in bone cancer pain and cause elevated levels of serum bone alkaline phosphatase (BAP) and carboxyterminal pyridlinoline cross-linked telopeptide of type 1 collagen (ICTP)...
  24. Impaired intestinal barrier function and airway inflammation in cystic fibrosis
    Robert C De Lisle; Fiscal Year: 2010
    ..central hypothesis of this project is that impaired intestinal innate defenses in CF, including decreased alkaline phosphatase (IAP) activity, compromised gut barrier function, and small intestinal bacterial overgrowth, contribute to ..
  25. Purinergic receptors in inflammation
    Wolfgang G Junger; Fiscal Year: 2012
    ..adenosine receptors are recruited to the leading edge where adenosine is generated by CD39/E- NTPDase1 and alkaline phosphatase (ALP)...
  26. Sleeping Beauty-Mediated Gene Therapy for Hemophilia
    R Scott McIvor; Fiscal Year: 2013
    ..after delivery of SB transposons to the liver of normal dogs, using DGI's unique canine secreted alkaline phosphatase (cSEAP) reporter system;(iii) To deliver SB transposons encoding canine clotting factor IX (cFIX) to the ..
  27. TNSALP Mutations in Atypical Femoral Fractures with Long-Term Bisphosphonate Use
    STEVEN R MUMM; Fiscal Year: 2013
    ..a heritable metabolic bone disease, is caused by loss-of-function mutations in the tissue non-specific alkaline phosphatase (TNSALP) gene, and therefore features low serum levels of alkaline phosphatase...
  28. Differentiation and Elimination of Chemo-surviving Colon Tumors
    ADHIP N MAJUMDAR; Fiscal Year: 2013
    ..of miR-21 leads to differentiation of these cells, as evidenced by increased CK-20 expression and alkaline phosphatase activity...
  29. Study of Enzymatic Phosphoryl Transfer
    Daniel Herschlag; Fiscal Year: 2012
    ..This proposal focuses on the alkaline phosphatase (AP) superfamily, aiming to understand the underlying structural and functional reasons why different ..
  30. Osteogenic Maturation and Bone Repair Require 1,25- and 24,25-dihydroxyvitamin D
    KEVIN MATTHEW CURTIS; Fiscal Year: 2012
    ..Our data establishes that 24,25OHD inhibits proliferation, increases alkaline phosphatase activity and mineralization, and decreases 1[unreadable]-hydroxylase expression, potentially decreasing the ..
  31. Crevicular Fluid Content During Early Wound Healing- Implant versus Tooth
    Binnaz Leblebicioglu; Fiscal Year: 2010
    ..g. VEGF, TGF-b, IL-6, IL-10, MMP-8, MMP-9, TIMP-1, TIMP-2, TNF-a, IL-1b, bone alkaline phosphatase and osteocalcin) to determine: a) anticipated differences in early wound healing responses at soft and/or ..
  32. Role of inflammatory response in aortic valve lesions
    Xianzhong Meng; Fiscal Year: 2013
    ..that BMP-2 plays a major role in TLR4-induced osteogenic responses, characterized by expression of Runx2, alkaline phosphatase (ALP) and osteopontin...
  33. Lysyl Oxidase Control of TGF-? in Bone
    Mitsuo Yamauchi; Fiscal Year: 2010
    ..To investigate the effect of LOX enzyme on TGF-[unreadable]1 signaling and its outcome (alkaline phosphatase activity, collagen synthesis/organization, mineralization) in vitro (2a) and ex vivo (2b)...
  34. Intracellular Membrane Fusion Mediated by SNARE Proteins
    Christopher Peters; Fiscal Year: 2013
    ..efficiency of vacuoles can be easily measured by using two different strains, one lacking the vacuolar alkaline phosphatase PHO8, the other lacking the vacuolar protease PEP4...
  35. Lead Optimization of Novel Inhibitors of Tissue Non-specific Alkaline Phosphatase
    Jose Luis Millan; Fiscal Year: 2013 applicant): This R01 application entitled "Lead Optimization of Novel Inhibitors of Tissue Non-specific Alkaline Phosphatase" is in response to PAR-12-060 "Solicitation of Validated Hits for the Discovery of in vivo Chemical Probes"...
    Marja Hurley; Fiscal Year: 2003
    ..FGF-2 on gene markers of differentiation of early and mature osteoblasts including, CBFA1, type 1 collagen, alkaline phosphatase and osteocalcin...
    Daniel Carson; Fiscal Year: 2004
    ..hypertrophic growth, a phase in which they express other marker proteins, including collagen type X and alkaline phosphatase. Molecular controls over chondrogenesis are manifest at many levels including the expression of key growth ..
  38. Phase 2 Study of ENB-0040 for the Treatment of Hypophosphatasia
    HORACIO B PLOTKIN; Fiscal Year: 2013
    ..This enzyme, called tissue non-specific alkaline phosphatase, is essential for the proper formation of bones...
    William Konigsberg; Fiscal Year: 1999
    ..The specific protein molecules under investigation are: 1) alkaline phosphatase; 2) the fungal transcription factors GAL4, LAC9, MAL63, LEU3 and HAP1; 3) the glucocorticoid and estrogen ..
  40. Mechanisms of initiation of skeletal mineralization
    Jose Luis Millan; Fiscal Year: 2013
    ..Our work centers on elucidating the concerted action of three phosphatases, PHOSPHO1, tissue-nonspecific alkaline phosphatase (TNAP) and nucleosidetriphosphate pyrophosphohydrolase (NPP1) in establishing a Pi/PPi ratio conducive to ..
  41. InstaCortisol:A realtime and continuous assessment of Cortisol in ISF
    MANJU GROVER VENUGOPAL; Fiscal Year: 2010
    ..voltammetry, when Cortisol sandwiches between the capture and the detector antibody, which is attached to Alkaline Phosphatase enzyme. There are four specific aims of this project...
    Dana Gaddy; Fiscal Year: 2003
    ..acts on early osteoblast progenitors to prevent their commitment to pre-osteoblasts capable of expressing alkaline phosphatase (AP). INH suppression of osteoblastogenesis is associated with decreased osteoclastogenesis...
    Virginia Kimonis; Fiscal Year: 2003
    ..Laboratory investigation indicates elevated alkaline phosphatase levels in affected individuals. CPK is normal to mildly elevated...
    MILTON HEITMANCIK; Fiscal Year: 1999
    ..studies to be conducted at interim sacrifices include hematology; serum calcium, phosphorus, fluoride and alkaline phosphatase; and bone fluoride. Serum and bone fluoride concentrations are to be measured at terminal sacrifice...
    David Alpers; Fiscal Year: 2000
    Intestinal alkaline phosphatase (IAP) in the rat provides a model for studying a unique mechanism of protein secretion from the enterocyte, in that IAP isomers I and II are secreted from the cell bound to a phospholipid-rich surfactant-..
    David Busbee; Fiscal Year: 1991
    ..Active DNA pol-a may be inactivated by treatment with alkaline phosphatase. Fibroblasts treated with the carcinogen r-7,t-8-dihydroxy-t-9,10- epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene (..
    Norman Sussman; Fiscal Year: 1993
    Serum alkaline phosphatase (AP) is an important marker of cholestasis, a condition in which the normal flow of bile from the liver is interrupted...
    Rong Guan; Fiscal Year: 2000
    ..of 055 in SW620 increased expression of several cell differentiation markers such as E-cadherin, CEA and alkaline phosphatase and induced morphological changes consistent with cell differentiation...
    WING KAM; Fiscal Year: 1993
    Human alkaline phosphatase is an important multigene family with as many as five tissue-specific forms...
    Jeffrey Gimble; Fiscal Year: 2005
    ..the literature suggest that a peripheral, "osteogenic" circadian clock exists; serum levels of osteocalcin, alkaline phosphatase, and C- telopeptide exhibit a reproducible, oscillatory diurnal pattern in human subjects and animal models...
    Ira Goldfine; Fiscal Year: 2000
    ..purification and partial sequencing led to the identification of PC-1 as nucleotide pyrophosphatase/alkaline phosphatase. A surface ectoenzyme of uncertain function but perhaps involved in recycling of nucleotide phosphates by ..
    Evan Kantrowitz; Fiscal Year: 2001
    ..The model system to be used for this project is alkaline phosphatase, an enzyme that catalyzes the nonspecific hydrolysis of phosphate esters...
  53. Anabolic Effect of Osteogenic Peptides
    Gary Balian; Fiscal Year: 2009
    ..We have shown this by measuring increases in alkaline phosphatase activity and gene expression for osteocalcin, bone sialoprotein, osterix and RUNX2, moreover, the peptides ..
  54. The Insulitis Reporter Mouse
    Jonathan Katz; Fiscal Year: 2007
    ..Soluble human placental alkaline phosphatase (sHPAP) is an ideal candidate...
    Beatrice Mintz; Fiscal Year: 1990
    ..The earliest events involve a set of alkaline phosphatase genes which will be isolated and characterized...
    Antonios Mikos; Fiscal Year: 2000
    ..b>Alkaline phosphatase activity, collagen synthesis, and formation of mineral deposits by cultured osteoblasts in 3-D will be used ..
    HOWARD SUSSMAN; Fiscal Year: 1992
    ..The region of DNA upstream from the transcriptional start of each of the alkaline phosphatase isoenzyme genes will be isolated and sequenced, and specific enhancers identified by testing native and ..
    Carol Ziomek; Fiscal Year: 1991
    b>Alkaline phosphatase (APase) is expressed in the preimplantation mouse embryo in a stage- and then a tissue-specific manner...
    J Whittaker; Fiscal Year: 1992, muscle expression which seems to depend on maternally-derived gene activator(s), and endodermal alkaline phosphatase development which apparently involves a maternal mRNA for the enzyme...
    MALCOLM STEINBERG; Fiscal Year: 1991
    ..The molecule is identified as the cell-surface enzyme alkaline phosphatase (ALP). A prime focus of this project is to understand the role of ALP in guiding migrating embryonic cells...
    IRENA BRONSTEIN; Fiscal Year: 1990
    ..The viability of DNA sequence imaging using a novel alkaline phosphatase labile dioxetane disodium 3-(4-methoxyspiro[1,2-dioxetane-3,2-tricyclo [
  62. Capillary Electrophoretic Studies of Enzyme Inhibition
    SAMUEL GILMAN; Fiscal Year: 2007
    ..Work with alkaline phosphatase will focus on continuing the development of quantitative models of the capillary electrophoretic enzyme ..
    Teresita Bellido; Fiscal Year: 2001
    ..Furthermore, acting via their specific receptors IL-6 type cytokines increase alkaline phosphatase and inhibit cell growth, suggesting their ability to induce differentiation...
    JOHN VOYTA; Fiscal Year: 1990
    ..The system will utilize a chemiluminescent substrate for alkaline phosphatase, AMPPD, which provides state of the art detection capabilities...
    TIAN TSONG; Fiscal Year: 1980
    ..2) Metal activation process of alkaline phosphatase from E. Coli...
    CHARLES RUTHERFORD; Fiscal Year: 1980 continue our fractionation of tumor homogenates on DEAE-Sephacel in order to separate isozymes of both alkaline phosphatase and cyclic nucleotide phosphodiesterase...
    Ellis Golub; Fiscal Year: 1999
    ..only components common to all vertebrate mineralized tissues are calcium and phosphate ions and the enzyme, alkaline phosphatase. While the beginning of this investigation will focus on alkaline phosphatase and matrix vesicles, the ..
    JOSEPH COLEMAN; Fiscal Year: 1991
    ..1) Continuation of a detailed correlation of crystal and solution structure for alkaline phosphatase of E. coli...