exoribonucleases

Summary

Summary: A family of enzymes that catalyze the exonucleolytic cleavage of RNA. It includes EC 3.1.13.-, EC 3.1.14.-, EC 3.1.15.-, and EC 3.1.16.-. EC 3.1.-

Top Publications

  1. pmc Structure of the Lassa virus nucleoprotein reveals a dsRNA-specific 3' to 5' exonuclease activity essential for immune suppression
    Kathryn M Hastie
    Departments of Immunology and Microbial Science and Molecular Biology, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 108:2396-401. 2011
  2. ncbi A single subunit, Dis3, is essentially responsible for yeast exosome core activity
    Andrzej Dziembowski
    Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique UPR2167, associée à l Université Pierre et Marie Curie, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Nat Struct Mol Biol 14:15-22. 2007
  3. pmc The exosome contains domains with specific endoribonuclease, exoribonuclease and cytoplasmic mRNA decay activities
    Daneen Schaeffer
    Department of Microbiology and Molecular Genetics, University of Texas Health Science Center Houston, 6431 Fannin Street, MSB 1 212 Houston, Texas 77030, USA
    Nat Struct Mol Biol 16:56-62. 2009
  4. doi Endonucleolytic RNA cleavage by a eukaryotic exosome
    Alice Lebreton
    Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, CNRS UPR 2167, 91198 Gif sur Yvette, France
    Nature 456:993-6. 2008
  5. pmc The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination
    Syuzo Kaneko
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Genes Dev 21:1779-89. 2007
  6. doi Active turnover modulates mature microRNA activity in Caenorhabditis elegans
    Saibal Chatterjee
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, CH 4002 Basel, Switzerland
    Nature 461:546-9. 2009
  7. pmc Structural and biochemical studies of the 5'→3' exoribonuclease Xrn1
    Jeong Ho Chang
    Department of Biological Sciences, Columbia University, New York, NY, USA
    Nat Struct Mol Biol 18:270-6. 2011
  8. ncbi 5'-to-3' exoribonuclease activity in bacteria: role of RNase J1 in rRNA maturation and 5' stability of mRNA
    Nathalie Mathy
    CNRS UPR 9073 affiliated with Université de Paris 7 Denis Diderot, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris
    Cell 129:681-92. 2007
  9. ncbi The Sm-like protein Hfq regulates polyadenylation dependent mRNA decay in Escherichia coli
    Bijoy K Mohanty
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Mol Microbiol 54:905-20. 2004
  10. pmc The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosome
    Claudia Schneider
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Nucleic Acids Res 37:1127-40. 2009

Research Grants

Detail Information

Publications237 found, 100 shown here

  1. pmc Structure of the Lassa virus nucleoprotein reveals a dsRNA-specific 3' to 5' exonuclease activity essential for immune suppression
    Kathryn M Hastie
    Departments of Immunology and Microbial Science and Molecular Biology, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 108:2396-401. 2011
    ..Thus, the nucleoprotein is a viral exonuclease with anti-immune activity, and this work provides a unique opportunity to combat arenaviral infections...
  2. ncbi A single subunit, Dis3, is essentially responsible for yeast exosome core activity
    Andrzej Dziembowski
    Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique UPR2167, associée à l Université Pierre et Marie Curie, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Nat Struct Mol Biol 14:15-22. 2007
    ..We observed that the yeast exosome ring mediates interactions with protein partners, providing an explanation for its essential function...
  3. pmc The exosome contains domains with specific endoribonuclease, exoribonuclease and cytoplasmic mRNA decay activities
    Daneen Schaeffer
    Department of Microbiology and Molecular Genetics, University of Texas Health Science Center Houston, 6431 Fannin Street, MSB 1 212 Houston, Texas 77030, USA
    Nat Struct Mol Biol 16:56-62. 2009
    ..The combination of an endoRNase and an exoRNase activity seems to be a widespread feature of RNA-degrading machines...
  4. doi Endonucleolytic RNA cleavage by a eukaryotic exosome
    Alice Lebreton
    Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, CNRS UPR 2167, 91198 Gif sur Yvette, France
    Nature 456:993-6. 2008
    ..The mode of action of eukaryotic exosome cores in RNA processing and degradation should be reconsidered, taking into account the cooperation between its multiple ribonucleolytic activities...
  5. pmc The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination
    Syuzo Kaneko
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Genes Dev 21:1779-89. 2007
    ..Together, our data indicate that p54nrb/PSF functions in recruitment of XRN2 to facilitate pre-mRNA 3' processing and transcription termination...
  6. doi Active turnover modulates mature microRNA activity in Caenorhabditis elegans
    Saibal Chatterjee
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, CH 4002 Basel, Switzerland
    Nature 461:546-9. 2009
    ..This pathway might represent a potential target for therapeutic intervention on miRNA expression...
  7. pmc Structural and biochemical studies of the 5'→3' exoribonuclease Xrn1
    Jeong Ho Chang
    Department of Biological Sciences, Columbia University, New York, NY, USA
    Nat Struct Mol Biol 18:270-6. 2011
    The 5'→3' exoribonucleases (XRNs) have important functions in transcription, RNA metabolism and RNA interference...
  8. ncbi 5'-to-3' exoribonuclease activity in bacteria: role of RNase J1 in rRNA maturation and 5' stability of mRNA
    Nathalie Mathy
    CNRS UPR 9073 affiliated with Université de Paris 7 Denis Diderot, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris
    Cell 129:681-92. 2007
    ..We show that a 30S ribosomal subunit bound to a Shine Dalgarno-like element (Stab-SD) in the cryIIIA mRNA blocks exonucleolytic progression of RNase J1, accounting for the stabilizing effect of this element in vivo...
  9. ncbi The Sm-like protein Hfq regulates polyadenylation dependent mRNA decay in Escherichia coli
    Bijoy K Mohanty
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Mol Microbiol 54:905-20. 2004
    ..Analysis of mRNA half-lives in hfq, deltapcnB and hfq deltapcnB mutants suggests that Hfq and PAP I function in the same mRNA decay pathway...
  10. pmc The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosome
    Claudia Schneider
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Nucleic Acids Res 37:1127-40. 2009
    ..Finally, the N-terminal PIN domain was shown to be necessary and sufficient for association with the core exosome, indicating its dual function as a nuclease and structural element...
  11. doi Human Ccr4-Not complexes contain variable deadenylase subunits
    Nga Chi Lau
    Department of Physiological Chemistry, University Medical Center Utrecht, Utrecht, The Netherlands
    Biochem J 422:443-53. 2009
    ..Taken together, human Ccr4-Not complexes are heterogeneous in composition owing to differences in their deadenylase subunits, which may reflect the multi-functionality of these complexes in cellular processes...
  12. ncbi Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts
    Lidia Vasiljeva
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Mol Cell 21:239-48. 2006
    ..Since Nrd1 is known to bind RNA polymerase II and be important for sn/snoRNA 3' end processing, Nrd1 may link transcription and RNA 3' end formation with surveillance by the exosome...
  13. pmc Infidelity of SARS-CoV Nsp14-exonuclease mutant virus replication is revealed by complete genome sequencing
    Lance D Eckerle
    Department of Pediatrics and Microbiology and Immunology and Elizabeth B Lamb Center for Pediatric Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    PLoS Pathog 6:e1000896. 2010
    ....
  14. ncbi A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans
    Scott Kennedy
    Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 427:645-9. 2004
    ..Thus, ERI-1 is a negative regulator that may normally function to limit the duration, cell-type specificity or endogenous functions of RNA interference...
  15. pmc The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels
    K F Jensen
    Institute of Biological Chemistry, University of Copenhagen, Denmark
    J Bacteriol 175:3401-7. 1993
    ....
  16. pmc Polyadenylation linked to transcription termination directs the processing of snoRNA precursors in yeast
    Pawel Grzechnik
    Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawinskiego 5A, 02 106 Warsaw, Poland
    Mol Cell 32:247-58. 2008
    ..We propose a model in which polyadenylation of pre-snoRNAs is a key event linking their transcription termination, 3' end processing, and degradation...
  17. pmc Structure and function of the 5'-->3' exoribonuclease Rat1 and its activating partner Rai1
    Song Xiang
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Nature 458:784-8. 2009
    The 5'-->3' exoribonucleases (XRNs) comprise a large family of conserved enzymes in eukaryotes with crucial functions in RNA metabolism and RNA interference...
  18. ncbi Distinct pathways for snoRNA and mRNA termination
    Minkyu Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 24:723-34. 2006
    ..These results suggest that in yeast the transcribing RNA polymerase II can choose between two distinct termination mechanisms but keeps both options available during elongation...
  19. pmc Budding yeast RNA polymerases I and II employ parallel mechanisms of transcriptional termination
    Junya Kawauchi
    Sir William Dunn School of Pathology, Oxford OX1 3RE, United Kingdom
    Genes Dev 22:1082-92. 2008
    ....
  20. ncbi Cryptic pol II transcripts are degraded by a nuclear quality control pathway involving a new poly(A) polymerase
    Françoise Wyers
    Equipe labelisée La Ligue, Avenue de la Terrasse, 91190 Gif sur Yvette, Paris, France
    Cell 121:725-37. 2005
    ..Our data strongly support the existence of a posttranscriptional quality control mechanism limiting inappropriate expression of genetic information...
  21. pmc Identification of a quality-control mechanism for mRNA 5'-end capping
    Xinfu Jiao
    Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854, USA
    Nature 467:608-11. 2010
    ..We propose that Rai1 is involved in an as yet uncharacterized quality control process that ensures mRNA 5'-end integrity by an aberrant-cap-mediated mRNA decay mechanism...
  22. pmc RNA polyadenylation and degradation in different Archaea; roles of the exosome and RNase R
    Victoria Portnoy
    Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Nucleic Acids Res 34:5923-31. 2006
    ..This ribonuclease is not able to degrade structured RNA better than PNPase. RNase R, which appears to be the only exoribonucleases in Haloferax volcanii, was found to be essential for viability.
  23. ncbi Unravelling the dynamics of RNA degradation by ribonuclease II and its RNA-bound complex
    Carlos Frazao
    Division of Biological Chemistry, ITQB Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apt 127, 2781 901 Oeiras, Portugal
    Nature 443:110-4. 2006
    ..Moreover, it reveals important structural features that can be extrapolated to other members of this family...
  24. pmc Efficient termination of transcription by RNA polymerase I requires the 5' exonuclease Rat1 in yeast
    Aziz El Hage
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
    Genes Dev 22:1069-81. 2008
    ..When Rat1 catches Pol I, which is predicted to be paused at T1, transcription is terminated...
  25. pmc Coupled 5' nucleotide recognition and processivity in Xrn1-mediated mRNA decay
    Martin Jinek
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 41:600-8. 2011
    ....
  26. ncbi RNase II levels change according to the growth conditions: characterization of gmr, a new Escherichia coli gene involved in the modulation of RNase II
    F Cairrao
    Instituto Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apart 127, 2781 901 Oeiras, Portugal
    Mol Microbiol 39:1550-61. 2001
    ..The data presented here show that the regulation of ribonucleolytic activity can depend on growth conditions, and this regulation can be mediated by factors that are not RNases...
  27. pmc Human mitochondrial SUV3 and polynucleotide phosphorylase form a 330-kDa heteropentamer to cooperatively degrade double-stranded RNA with a 3'-to-5' directionality
    Dennis Ding Hwa Wang
    Department of Biological Chemistry, University of California, Irvine, California 92697, USA
    J Biol Chem 284:20812-21. 2009
    ..Taken together, these results strongly suggest that the complex of hSUV3-hPNPase is an integral entity for efficient degradation of structured RNA and may be the long sought RNA-degrading complex in the mammalian mitochondria...
  28. doi The role of 3'-5' exoribonucleases in RNA degradation
    José M Andrade
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Qeiras, Portugal
    Prog Mol Biol Transl Sci 85:187-229. 2009
    ..Escherichia coli has eight 3'-5' exoribonucleases, which are involved in multiple RNA metabolic pathways...
  29. ncbi Identification of 2'-phosphodiesterase, which plays a role in the 2-5A system regulated by interferon
    Kazuishi Kubota
    Biomedical Research Laboratories, Sankyo Co, Ltd, 1 2 58, Hiromachi, Shinagawa ku, Tokyo 140 8710, Japan
    J Biol Chem 279:37832-41. 2004
    ..These observations identify 2'-PDE as a key regulator of the 2-5A system and as a potential novel target for antiviral and antitumor treatments...
  30. pmc 5'-end surveillance by Xrn2 acts as a shared mechanism for mammalian pre-rRNA maturation and decay
    Minshi Wang
    Department of Cell Biology, University of Medicine and Dentistry of New Jersey, Stratford, NJ 08084, USA
    Nucleic Acids Res 39:1811-22. 2011
    ..We propose that probing of pre-rRNA maturation intermediates by exonucleases serves the dual function of generating mature rRNAs and suppressing suboptimal processing paths during ribosome assembly...
  31. pmc The 1.4-A crystal structure of the S. pombe Pop2p deadenylase subunit unveils the configuration of an active enzyme
    Anette Thyssen Jonstrup
    Centre for Structural Biology, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Arhus C, Denmark
    Nucleic Acids Res 35:3153-64. 2007
    ....
  32. ncbi Facilitation of mRNA deadenylation and decay by the exosome-bound, DExH protein RHAU
    Hoanh Tran
    Friedrich Miescher Institute for Biomedical Research, Novartis Research Foundation, Maulbeerstrasse 66, CH 4058 Basel, Switzerland
    Mol Cell 13:101-11. 2004
    ..that either stabilize or otherwise degrade ARE-mRNAs by recruiting the exosome, a complex of 3'-to-5' exoribonucleases. We have identified RHAU, a putative DExH RNA helicase that was isolated in association with the ARE of ..
  33. doi The yeast 5'-3' exonuclease Rat1p functions during transcription elongation by RNA polymerase II
    Silvia Jimeno-González
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, Aarhus University, Aarhus DK 8000, Denmark
    Mol Cell 37:580-7. 2010
    ..Our data suggest that Rat1p serves to terminate RNAPII molecules engaged in the production of uncapped RNA, regardless of their position on the gene locus...
  34. pmc RNase MRP cleaves the CLB2 mRNA to promote cell cycle progression: novel method of mRNA degradation
    Tina Gill
    Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
    Mol Cell Biol 24:945-53. 2004
    ..In addition, these results denote a new mechanism of mRNA degradation not seen before in the yeast Saccharomyces cerevisiae...
  35. pmc Quality control of ribosomal RNA mediated by polynucleotide phosphorylase and RNase R
    Zhuan Fen Cheng
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, FL 33101, USA
    Proc Natl Acad Sci U S A 100:6388-93. 2003
    ..In the absence of these RNases, rRNA fragments accumulate, leading to interference with ribosome maturation and ultimately to cell death...
  36. pmc Dis3-like 1: a novel exoribonuclease associated with the human exosome
    Raymond H J Staals
    Department of Biomolecular Chemistry, Nijmegen Center for Molecular Life Sciences, Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, The Netherlands
    EMBO J 29:2358-67. 2010
    ..Taken together, these data indicate that hDis3L1 is a novel exosome-associated exoribonuclease in the cytoplasm of human cells...
  37. pmc Lithium toxicity in yeast is due to the inhibition of RNA processing enzymes
    B Dichtl
    EMBL, Gene Expression Programme, Postfach 10 2209, 69012 Heidelberg, Germany
    EMBO J 16:7184-95. 1997
    ..Here we report that pAp accumulation in HAL2 mutants inhibits the 5'-->3' exoribonucleases Xrn1p and Rat1p...
  38. pmc The 5' external transcribed spacer in mouse ribosomal RNA contains two cleavage sites
    Tatyana Kent
    Department of Cell Biology, School of Osteopathic Medicine, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    RNA 15:14-20. 2009
    ..We discuss the finding of a second processing site in mammalian 5'ETS in relation to the involvement of the U3 snoRNA in pre-rRNA processing and present a revised map of the mouse 18S rRNA processing pathway...
  39. ncbi Decay of endoplasmic reticulum-localized mRNAs during the unfolded protein response
    Julie Hollien
    Department of Cellular and Molecular Pharmacology, University of California San Francisco, Howard Hughes Medical Institute, San Francisco, CA 94143, USA
    Science 313:104-7. 2006
    ..This response is well suited to complement other UPR mechanisms because it could selectively halt production of proteins that challenge the ER and clear the translocation and folding machinery for the subsequent remodeling process...
  40. pmc Transcription termination by nuclear RNA polymerases
    Patricia Richard
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Genes Dev 23:1247-69. 2009
    ..In this review, we describe the present state of knowledge on how each of the three RNA polymerases terminates and how mechanisms are conserved, or vary, from yeast to human...
  41. ncbi Genome-wide analysis of mRNAs regulated by the nonsense-mediated and 5' to 3' mRNA decay pathways in yeast
    Feng He
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Mol Cell 12:1439-52. 2003
    ....
  42. ncbi Antisense RNA stabilization induces transcriptional gene silencing via histone deacetylation in S. cerevisiae
    Jurgi Camblong
    Department of Cell Biology, Sciences III, University of Geneva, 30 quai E Ansermet, 1211 Geneva 4, Switzerland
    Cell 131:706-17. 2007
    ....
  43. ncbi Genome-wide high-resolution mapping of exosome substrates reveals hidden features in the Arabidopsis transcriptome
    Julia A Chekanova
    School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110, USA
    Cell 131:1340-53. 2007
    ..These first genome-wide maps of exosome substrates will aid in illuminating new fundamental components and regulatory mechanisms of eukaryotic transcriptomes...
  44. ncbi Polynucleotide phosphorylase, RNase II and RNase E play different roles in the in vivo modulation of polyadenylation in Escherichia coli
    B K Mohanty
    Department of Genetics, University of Georgia, Athens, GA 30605, USA
    Mol Microbiol 36:982-94. 2000
    ..Conversely, toxicity is significantly reduced in the presence of excess RNase II. Overproduction of RNase E leads to increased polyadenylation and no reduction in toxicity...
  45. pmc Pause sites promote transcriptional termination of mammalian RNA polymerase II
    Natalia Gromak
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Mol Cell Biol 26:3986-96. 2006
    ..Taken together, we provide new insights into the mechanism of pause site-mediated termination and establish a general role for the 5'-->3' exonuclease Xrn2 in Pol II termination...
  46. pmc Degradation of microRNAs by a family of exoribonucleases in Arabidopsis
    Vanitharani Ramachandran
    Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California Riverside, Riverside, CA 92521, USA
    Science 321:1490-2. 2008
    ..Here, we show that a family of exoribonucleases encoded by the SMALL RNA DEGRADING NUCLEASE (SDN) genes degrades mature miRNAs in Arabidopsis...
  47. pmc Bacillus subtilis YhaM, a member of a new family of 3'-to-5' exonucleases in gram-positive bacteria
    Irina A Oussenko
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine of New York University, New York 10029, USA
    J Bacteriol 184:6250-9. 2002
    A strain of Bacillus subtilis lacking two 3'-to-5' exoribonucleases, polynucleotide phosphorylase (PNPase) and RNase R, was used to purify another 3'-to-5' exoribonuclease, which is encoded by the yhaM gene...
  48. pmc Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1
    Irina Chernyakov
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
    Genes Dev 22:1369-80. 2008
    ..The RTD pathway is the first to be implicated in the turnover of mature RNA species from the class of stable RNAs. These results and the results of others demonstrate that tRNA, like mRNA, is subject to multiple quality control steps...
  49. pmc Novel features of the XRN-family in Arabidopsis: evidence that AtXRN4, one of several orthologs of nuclear Xrn2p/Rat1p, functions in the cytoplasm
    J P Kastenmayer
    Michigan State University, Department of Energy Plant Research Laboratory, and Biochemistry Department, 322 Plant Biology Building, East Lansing, MI 48824 1312, USA
    Proc Natl Acad Sci U S A 97:13985-90. 2000
    The 5'-3' exoribonucleases Xrn1p and Xrn2p/Rat1p function in the degradation and processing of several classes of RNA in Saccharomyces cerevisiae...
  50. ncbi Nuclear retention of unspliced mRNAs in yeast is mediated by perinuclear Mlp1
    Vincent Galy
    Unité de Biologie Cellulaire du Noyau, CNRS URA 2582, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Cell 116:63-73. 2004
    ..We propose that at this confined site the perinuclear Mlp1 implements a quality control step prior to export, physically retaining faulty pre-mRNAs...
  51. pmc Exoribonuclease R in Mycoplasma genitalium can carry out both RNA processing and degradative functions and is sensitive to RNA ribose methylation
    Maureen S Lalonde
    Department of Biomedical Science, Florida Atlantic University, Boca Raton, FL 33431, USA
    RNA 13:1957-68. 2007
    ..In contrast, EcR degrades this pre-tRNA without the formation of specific products. Our results suggest that MgR shares some properties of both EcR and EcII and can carry out a broad range of RNA processing and degradative functions...
  52. pmc Serum-deprivation stimulates cap-binding by PARN at the expense of eIF4E, consistent with the observed decrease in mRNA stability
    Ruth Seal
    Department of Neurology, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK
    Nucleic Acids Res 33:376-87. 2005
    ..These phosphorylation-induced changes in the interplay of PARN and eIF4E may determine whether the mRNA is translated or decayed...
  53. ncbi Structural basis for processivity and single-strand specificity of RNase II
    Yuhong Zuo
    Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, P O Box 016129, Miami, FL 33101, USA
    Mol Cell 24:149-56. 2006
    RNase II is a member of the widely distributed RNR family of exoribonucleases, which are highly processive 3'-->5' hydrolytic enzymes that play an important role in mRNA decay. Here, we report the crystal structure of E...
  54. ncbi Mechanism of action of RNase T. II. A structural and functional model of the enzyme
    Yuhong Zuo
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33101 6129, USA
    J Biol Chem 277:50160-4. 2002
    ..These data explain why RNase T must dimerize to function. The model provides a detailed framework on which to explain the mechanism of action of RNase T...
  55. ncbi The transcription factor associated Ccr4 and Caf1 proteins are components of the major cytoplasmic mRNA deadenylase in Saccharomyces cerevisiae
    M Tucker
    Department of Molecular and Cellular Biology and, Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Cell 104:377-86. 2001
    ..Interestingly, because Ccr4p and Caf1p interact with transcription factors, these results suggest an unexpected link between mRNA synthesis and turnover...
  56. pmc Exonucleolysis is required for nuclear mRNA quality control in yeast THO mutants
    Jannie Assenholt
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, The Faculty of Science, Aarhus University, DK 8000 Aarhus C, Denmark
    RNA 14:2305-13. 2008
    ..Our data show that exonucleolytic attack by the nuclear exosome is needed both for provoking mRNP QC and for its ensuing elimination of faulty RNA...
  57. ncbi Inhibition of human poly(A)-specific ribonuclease (PARN) by purine nucleotides: kinetic analysis
    Nikolaos A A Balatsos
    Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece
    J Enzyme Inhib Med Chem 24:516-23. 2009
    ..Our data imply that PARN activity can be modulated by purine nucleotides in vitro, providing an additional simple regulatory mechanism...
  58. ncbi RNase PH is essential for tRNA processing and viability in RNase-deficient Escherichia coli cells
    K O Kelly
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06030 3305
    J Biol Chem 267:16015-8. 1992
    ..These data indicate that RNase PH can be an essential enzyme for the processing of tRNA precursors...
  59. pmc Targeting of the cytosolic poly(A) binding protein PABPC1 to mitochondria causes mitochondrial translation inhibition
    Mateusz Wydro
    Mitochondrial Research Group, Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    Nucleic Acids Res 38:3732-42. 2010
    ..These data are consistent with endogenous RNA-binding factor(s) interacting with the poly(A) to optimize mitochondrial protein synthesis...
  60. pmc Maturation of 23S ribosomal RNA requires the exoribonuclease RNase T
    Z Li
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101, USA
    RNA 5:139-46. 1999
    ..Although other exoribonucleases can participate in shortening the 3' end of the initial RNase III cleavage product, RNase T is required for ..
  61. pmc Escherichia coli RNase R has dual activities, helicase and RNase
    Naoki Awano
    Department of Biochemistry, Robert Wood Johnson Medical School, UMDNJ, CABM, 679 Hoes Lane, Piscataway, New Jersey 08854, USA
    J Bacteriol 192:1344-52. 2010
    ..Interestingly, other major 3'-to-5' processing exoribonucleases of E...
  62. pmc Acetylation regulates the stability of a bacterial protein: growth stage-dependent modification of RNase R
    Wenxing Liang
    Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33101, USA
    Mol Cell 44:160-6. 2011
    ..These findings indicate that acetylation can regulate the stability of a bacterial protein...
  63. ncbi RNase II: the finer details of the Modus operandi of a molecular killer
    Cecilia M Arraiano
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    RNA Biol 7:276-81. 2010
    ..These exoribonucleases processively degrade RNA from the 3'-end releasing 5'-nucleotide monophosphates...
  64. pmc Structural components and architectures of RNA exosomes
    Kurt Januszyk
    Structural Biology Program, Sloan Kettering Institute, New York, New York, USA
    Adv Exp Med Biol 702:9-28. 2010
    ....
  65. pmc Maturation of mammalian H/ACA box snoRNAs: PAPD5-dependent adenylation and PARN-dependent trimming
    Heike Berndt
    Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, 06099 Halle, Germany
    RNA 18:958-72. 2012
    ..The noncanonical poly(A) polymerase PAPD5 is responsible for addition of the oligo(A) tails. We suggest that deadenylation is coupled to clean 3' end trimming, which might serve to enhance snoRNA stability...
  66. pmc Polyadenylation helps regulate functional tRNA levels in Escherichia coli
    Bijoy K Mohanty
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Nucleic Acids Res 40:4589-603. 2012
    ..Surprisingly, neither PNPase nor RNase II has any effect on tRNA poly(A) tail length. Our data suggest that the polyadenylation of tRNAs by PAP I likely proceeds in a distributive fashion unlike what is observed with mRNAs...
  67. doi Biochemical characterization of the RNase II family of exoribonucleases from the human pathogens Salmonella typhimurium and Streptococcus pneumoniae
    Susana Domingues
    Instituto de Tecnologia Quimica e Biologica Universidade Nova de Lisboa, Apartado 127, 2781 901 Oeiras, Portugal
    Biochemistry 48:11848-57. 2009
    ..Since ribonucleases have not been previously characterized in S. pneumoniae or Sa. typhimurium, this work provides an important first step in the understanding of post-transcriptional control in these pathogens...
  68. ncbi The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases
    P Mitchell
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 91:457-66. 1997
    ..Remarkably, four of these proteins are homologous to characterized bacterial 3'-->5' exoribonucleases; Rrp44p is homologous to RNase II, while Rrp41p, Rrp42p, and Rrp43p are related to RNase PH...
  69. pmc Nuclear deadenylation/polyadenylation factors regulate 3' processing in response to DNA damage
    Murat A Cevher
    Department of Chemistry, Hunter College, City University of New York, New York, NY, USA
    EMBO J 29:1674-87. 2010
    ....
  70. doi Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility
    Dewi Astuti
    Centre for Rare Diseases and Personalised Medicine, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, UK
    Nat Genet 44:277-84. 2012
    ..We also detected evidence of DIS3L2 mutations in sporadic Wilms tumor. These observations suggest that DIS3L2 has a critical role in RNA metabolism and is essential for the regulation of cell growth and division...
  71. ncbi Mutational analysis of an RNA internal loop as a reactivity epitope for Escherichia coli ribonuclease III substrates
    Irina Calin-Jageman
    Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA
    Biochemistry 42:5025-34. 2003
    ..The implications of these findings are discussed in light of RNase III substrate function as a gene regulatory element...
  72. pmc Poly(A) tail length control in Saccharomyces cerevisiae occurs by message-specific deadenylation
    C E Brown
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    Mol Cell Biol 18:6548-59. 1998
    ..The ability to control the length of the poly(A) tail for newly expressed mRNAs has the potential to be an important posttranscriptional regulatory step in gene expression...
  73. ncbi Differential sensitivities of portions of the mRNA for ribosomal protein S20 to 3'-exonucleases dependent on oligoadenylation and RNA secondary structure
    G A Coburn
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3
    J Biol Chem 271:15776-81. 1996
    ..Our data point to the importance of oligoadenylation in facilitating 3'-exonucleolytic activity and indicate that there are alternative degradative pathways. The implications for mRNA decay are discussed...
  74. pmc Rrp17p is a eukaryotic exonuclease required for 5' end processing of Pre-60S ribosomal RNA
    Marlene Oeffinger
    Rockefeller University, New York, NY 10065, USA
    Mol Cell 36:768-81. 2009
    ..We show that Rrp17p binds late pre-60S ribosomes, accompanying them from the nucleolus to the nuclear periphery, and provide evidence for physical and functional links between late 60S subunit processing and export...
  75. pmc Processing of the precursors to small nucleolar RNAs and rRNAs requires common components
    E Petfalski
    Institute of Cell and Molecular Biology, University of Edinburgh, United Kingdom
    Mol Cell Biol 18:1181-9. 1998
    ..8S rRNA. Processing of the pre-rRNA and both intronic and polycistronic snoRNAs therefore involves common components...
  76. pmc Deletion of the nuclear exosome component RRP6 leads to continued accumulation of the histone mRNA HTB1 in S-phase of the cell cycle in Saccharomyces cerevisiae
    Ruth Canavan
    Department of Microbiology, Moyne Institute for Preventive Medicine, Trinity College, University of Dublin, Dublin, Ireland
    Nucleic Acids Res 35:6268-79. 2007
    ....
  77. ncbi The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II
    Minkyu Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:517-22. 2004
    ..These findings support a model in which poly(A) site cleavage and subsequent degradation of the 3'-downstream RNA by Rat1 trigger transcription termination...
  78. pmc Determination of key residues for catalysis and RNA cleavage specificity: one mutation turns RNase II into a "SUPER-ENZYME"
    Ana Barbas
    Instituto de Tecnologia Quimica e Biologica Universidade Nova de Lisboa, 2781 901 Oeiras, Portugal
    J Biol Chem 284:20486-98. 2009
    ..The most prominent finding presented is the extraordinary catalysis observed in the E542A mutant that turns RNase II into a "super-enzyme."..
  79. doi Terminating transcription in yeast: whether to be a 'nerd' or a 'rat'
    Ana G Rondon
    Nat Struct Mol Biol 15:775-6. 2008
  80. pmc Recognition of adenosine residues by the active site of poly(A)-specific ribonuclease
    Niklas Henriksson
    Department of Cell and Molecular Biology, Uppsala University, Box 596, SE 751 24 Uppsala, Sweden
    J Biol Chem 285:163-70. 2010
    ..The poly(U)-degrading property of PARN could be of biological significance as oligo(U) tails recently have been proposed to play a role in RNA stabilization and destabilization...
  81. pmc Studies of the 5' exonuclease and endonuclease activities of CPSF-73 in histone pre-mRNA processing
    Xiao cui Yang
    Program in Molecular Biology and Biotechnology, CB 3280, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Cell Biol 29:31-42. 2009
    ..RNA interference experiments with HeLa cells indicate that degradation of the DCP does not depend on the Xrn2 5' exonuclease, suggesting that CPSF-73 degrades the DCP both in vitro and in vivo...
  82. pmc Human Pat1b connects deadenylation with mRNA decapping and controls the assembly of processing bodies
    Sevim Ozgur
    German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg, Germany
    Mol Cell Biol 30:4308-23. 2010
    ..Taken together, these findings provide evidence that human Pat1b is a central component of the RNA decay machinery by physically connecting deadenylation with decapping...
  83. pmc Arabidopsis thaliana XRN2 is required for primary cleavage in the pre-ribosomal RNA
    Monika Zakrzewska-Placzek
    Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Warsaw, Poland
    Nucleic Acids Res 38:4487-502. 2010
    ..We also show that some rRNA precursors and excised spacer fragments that accumulate in the absence of AtXRN2 and AtXRN3 are polyadenylated, indicating that these nucleases contribute to polyadenylation-dependent nuclear RNA surveillance...
  84. pmc Mechanism of RNA stabilization and translational activation by a pentatricopeptide repeat protein
    Jana Prikryl
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    Proc Natl Acad Sci U S A 108:415-20. 2011
    ..to its native binding site in the chloroplast atpI-atpH intergenic region (i) blocks both 5'→3' and 3'→ 5 exoribonucleases in vitro; (ii) is sufficient to define the native processed atpH mRNA 5'-terminus in conjunction with a ..
  85. pmc Arabidopsis FIERY1, XRN2, and XRN3 are endogenous RNA silencing suppressors
    Isabelle Gy
    Laboratoire de Biologie Cellulaire, Institut Jean Pierre Bourgin, Institut National de la Recherche Agronomique INRA, 78026 Versailles Cedex, France
    Plant Cell 19:3451-61. 2007
    ..Here, we show that the nuclear exoribonucleases XRN2 and XRN3 are endogenous PTGS suppressors...
  86. doi Yeast RNase III triggers polyadenylation-independent transcription termination
    Ghada Ghazal
    RNA Group, Departement de Microbiologie et d Infectiologie, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
    Mol Cell 36:99-109. 2009
    ..These results support a model for transcription termination in which cotranscriptional cleavage by Rnt1p provides access for exoribonucleases in the absence of polyadenylation signals.
  87. ncbi A 54-kDa fragment of the Poly(A)-specific ribonuclease is an oligomeric, processive, and cap-interacting Poly(A)-specific 3' exonuclease
    J Martinez
    Department of Cell and Molecular Biology, Uppsala University, Box 596, SE 751 24 Uppsala, Sweden
    J Biol Chem 275:24222-30. 2000
    ..Addition of free m(7)G(5')ppp(5')G cap analogue inhibited poly(A) degradation in vitro, suggesting a functional link between the RNA 5' end cap structure and poly(A) degradation at the 3' end of the RNA...
  88. ncbi Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents
    Philip L Ross
    Applied Biosystems, Framingham, MA 01701, USA
    Mol Cell Proteomics 3:1154-69. 2004
    ..We find that inactivation of Upf1p and Xrn1p causes common as well as unique effects on protein expression...
  89. pmc Translational repression by deadenylases
    Amy Cooke
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 285:28506-13. 2010
    ..The deadenylation-independent repression requires a 5' cap structure on the mRNA; however, deadenylation does not. We suggest that mere recruitment of CAF1 is sufficient for repression, independent of deadenylation...
  90. doi The RNA exosome component hRrp6 is a target for 5-fluorouracil in human cells
    Susanne Kammler
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, University of Aarhus, CF Møllers Alle, Building 1130, 8000 Aarhus C, Denmark
    Mol Cancer Res 6:990-5. 2008
    ..Taken together, our data position hRrp6 as a promising target for antiproliferative intervention...
  91. pmc Ribonuclease P processes polycistronic tRNA transcripts in Escherichia coli independent of ribonuclease E
    Bijoy K Mohanty
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Nucleic Acids Res 35:7614-25. 2007
    ..Our data indicate that RNase P substrate recognition is more complex than previously envisioned...
  92. ncbi Human 5' --> 3' exonuclease Xrn2 promotes transcription termination at co-transcriptional cleavage sites
    Steven West
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Nature 432:522-5. 2004
    ..Degradation of the downstream cleavage product by Xrn2 results in transcriptional termination, as envisaged in the torpedo model...
  93. doi Nop53p interacts with 5.8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome
    Daniela C Granato
    Department of Biochemistry, Institute of Chemistry, University of Sao Paulo, Brazil
    FEBS J 275:4164-78. 2008
    ..Consistent with this observation and similar to the observed in exosome mutants, depletion of Nop53p leads to accumulation of polyadenylated pre-rRNAs...
  94. ncbi The RNA processing exosome is linked to elongating RNA polymerase II in Drosophila
    Erik D Andrulis
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Nature 420:837-41. 2002
    ..Here we show that Drosophila Spt6 (dSpt6) co-purifies with the exosome, a complex of 3' to 5' exoribonucleases that is implicated in the processing of structural RNA and in the degradation of improperly processed pre-..
  95. ncbi Nonsense-mediated mRNA decay: molecular insights and mechanistic variations across species
    Elena Conti
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Curr Opin Cell Biol 17:316-25. 2005
    ..With the elucidation of the first crystal structures of components of the NMD machinery, the way is paved towards a molecular understanding of the protein interaction network underlying this process...
  96. pmc RNase activity of polynucleotide phosphorylase is critical at low temperature in Escherichia coli and is complemented by RNase II
    Naoki Awano
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    J Bacteriol 190:5924-33. 2008
    ....
  97. doi Exosome-mediated quality control: substrate recruitment and molecular activity
    Alice Lebreton
    Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, CNRS UPR2167 Associée à l Université P et M Curie, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Biochim Biophys Acta 1779:558-65. 2008
    ..Despite this avalanche of new results, further analyses will be required to improve our understanding of exosome regulation...
  98. ncbi 5'-exonuclease-2 of Saccharomyces cerevisiae. Purification and features of ribonuclease activity with comparison to 5'-exonuclease-1
    A Stevens
    Biology Division, Oak Ridge National Laboratory, Tennessee 37831 8080, USA
    J Biol Chem 270:16063-9. 1995
    ..5'-Exonuclease-1 hydrolyzes the synthetic RNAs and (pA)4 in an identical manner...
  99. pmc The exosome and RNA quality control in the nucleus
    Stepanka Vanacova
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, CH 4057 Basel, Switzerland
    EMBO Rep 8:651-7. 2007
    ..In this review, we discuss recent structural and functional data related to the nuclear quality-control apparatus, including the long-awaited structure of the human exosome and its activity...
  100. pmc Quantitative analysis of processive RNA degradation by the archaeal RNA exosome
    Sophia Hartung
    Center for Integrated Protein Sciences, Department of Biochemistry, Ludwig Maximilians University Munich, Munich, Germany
    Nucleic Acids Res 38:5166-76. 2010
    ....
  101. ncbi Defining the mechanism by which IFN-beta dowregulates c-myc expression in human melanoma cells: pivotal role for human polynucleotide phosphorylase (hPNPaseold-35)
    D Sarkar
    Department of Pathology, Herbert Irving Comprehensive Caner Center, Columbia University Medical Center, College of Physicians and Surgeons, New York, NY 10032, USA
    Cell Death Differ 13:1541-53. 2006
    ..In these contexts, targeted overexpression of hPNPase(old-35) might be a novel therapeutic strategy for c-myc-overexpressing and IFN-resistant tumors, such as melanomas...

Research Grants82

  1. Cis- and trans-acting factors in mitochondrial RNA decay
    LAURIE READ; Fiscal Year: 2009
    ..Two candidate exoribonucleases were identified in the T. brucei genome, and one was shown to be essential for optimal growth...
  2. Cis- and trans-acting factors in mitochondrial RNA decay
    Laurie K Read; Fiscal Year: 2010
    ..Two candidate exoribonucleases were identified in the T. brucei genome, and one was shown to be essential for optimal growth...
  3. INITIATION OF MRNA DECAY IN BACILLUS SUBTILIS
    DAVID BECHHOFER; Fiscal Year: 2009
    ..The availability of B. subtilis strains that are deficient in 1 or more 3'-to-5' exoribonucleases will make it possible to examine the role of individual ribonucleases in mRNA turnover...
  4. INITIATION OF MRNA DECAY IN BACILLUS SUBTILIS
    DAVID BECHHOFER; Fiscal Year: 2009
    ..The availability of B. subtilis strains that are deficient in 1 or more 3'-to-5' exoribonucleases will make it possible to examine the role of individual ribonucleases in mRNA turnover...
  5. INITIATION OF MRNA DECAY IN BACILLUS SUBTILIS
    DAVID BECHHOFER; Fiscal Year: 2007
    ..The availability of B. subtilis strains that are deficient in 1 or more 3'-to-5' exoribonucleases will make it possible to examine the role of individual ribonucleases in mRNA turnover...
  6. INITIATION OF MRNA DECAY IN BACILLUS SUBTILIS
    David H Bechhofer; Fiscal Year: 2010
    ..The availability of B. subtilis strains that are deficient in 1 or more 3'-to-5'exoribonucleases will make it possible to examine the role of individual ribonucleases in mRNA turnover...
  7. Analysis of E. coli ribonucleases and RNA metabolism
    SIDNEY KUSHNER; Fiscal Year: 2009
    ..seven endoribonucleases (RNase I, RNase III, RNase E, RNase G, RNase Z, RNase P, and RNase LS) as well as two exoribonucleases (polynucleotide phosphorylase, RNase II). Specific experiments include: 1...
  8. Analysis of E. coli ribonucleases and RNA metabolism
    Sidney R Kushner; Fiscal Year: 2010
    ..seven endoribonucleases (RNase I, RNase III, RNase E, RNase G, RNase Z, RNase P, and RNase LS) as well as two exoribonucleases (polynucleotide phosphorylase, RNase II). Specific experiments include: 1...
  9. MITOCHONDRIAL RNA METABOLISM IN TRYPANOSOMA BRUCEI
    LAURIE READ; Fiscal Year: 2004
    ..We will also focus on identification and isolation of exoribonucleases. Finally, we will use genetic and biochemical means to isolate T...
  10. POSTTRANSCRIPTIONAL REGULATION OF ONCOGENE MESSENGER RNA
    Gary Brewer; Fiscal Year: 2006
    ..Candidate 3'-5' activities include a deadenylase, such as PARN; the exosome, which is a large complex of exoribonucleases; and the proteasome, which appears to possess an ARE-specific ribonuclease activity...
  11. MITOCHONDRIAL RNA METABOLISM IN TRYPANOSOMA BRUCEI
    LAURIE READ; Fiscal Year: 2007
    ..We will identify poly(A)-selective exoribonucleases and characterize their function using RNAi-mediated targeted depletion...
  12. Structural and Functional Studies of Eukaryotic Exosomes
    CHRISTOPHER LIMA; Fiscal Year: 2009
    ..subunits led to the hypothesis that the exosome was composed of up to eleven distinct 3' to 5' exoribonucleases. However, recent studies utilizing reconstituted and affinity-purified cellular exosome complexes indicate ..
  13. Structural and Functional Studies of Eukaryotic Exosomes
    Christopher D Lima; Fiscal Year: 2010
    ..subunits led to the hypothesis that the exosome was composed of up to eleven distinct 3'to 5'exoribonucleases. However, recent studies utilizing reconstituted and affinity-purified cellular exosome complexes indicate ..
  14. Structural and Functional Studies of Eukaryotic Exosomes
    Christopher D Lima; Fiscal Year: 2011
    ..subunits led to the hypothesis that the exosome was composed of up to eleven distinct 3'to 5'exoribonucleases. However, recent studies utilizing reconstituted and affinity-purified cellular exosome complexes indicate ..
  15. ENZYMOLOGY AND CONTROL OF AMINO ACID ACTIVATION
    MURRAY DEUTSCHER; Fiscal Year: 2006
    ..coli exoribonucleases; 3) elucidation of exoribonuclease active sites, substrate recognition and mechanism of action; 4) ..
  16. Structural studies of exoribonucleases
    Arun Malhotra; Fiscal Year: 2007
    ..as a model system for understanding the role of ribonucleases in cellular RNA metabolism, and eight distinct exoribonucleases have been identified in this bacterium...
  17. ENZYMOLOGY AND CONTROL OF AMINO ACID ACTIVATION
    MURRAY DEUTSCHER; Fiscal Year: 2002
    ..coli exoribonucleases and their genes; 3) elucidation of exoribonuclease active sites and specificity of substrate recognition; 4) ..
  18. Compartmentalized Exosome Structure and Function
    Erik D Andrulis; Fiscal Year: 2010
    ..RNA metabolic pathways is the exosome, an essential protein complex comprising numerous 3'to 5'exoribonucleases. Yet, the exact mechanisms underlying how the exosome functions in these pathways are unclear...
  19. Structure and Function of the Decapping Enzyme Complex
    John D Gross; Fiscal Year: 2011
    ..sentences an mRNA for destruction by exposing the 5'monophosphate of the mRNA body to 5'to 3'exoribonucleases. The activity of Dcp2 is stimulated by a variety of pathway specific co-activators through mechanisms that ..
  20. Compartmentalized Exosome Structure and Function
    ERIK ANDRULIS; Fiscal Year: 2007
    ..distinct RNA metabolic pathways is the exosome, an essential protein complex comprising numerous 3' to 5' exoribonucleases. Yet, the exact mechanisms underlying how the exosome functions in these pathways are unclear...
  21. Structure and Function of the Decapping Enzyme Complex
    John D Gross; Fiscal Year: 2010
    ..sentences an mRNA for destruction by exposing the 5'monophosphate of the mRNA body to 5'to 3'exoribonucleases. The activity of Dcp2 is stimulated by a variety of pathway specific co-activators through mechanisms that ..
  22. Compartmentalized Exosome Structure and Function
    ERIK ANDRULIS; Fiscal Year: 2009
    ..RNA metabolic pathways is the exosome, an essential protein complex comprising numerous 3' to 5' exoribonucleases. Yet, the exact mechanisms underlying how the exosome functions in these pathways are unclear...
  23. Structure and Function of the Decapping Enzyme Complex
    John Gross; Fiscal Year: 2009
    ..an mRNA for destruction by exposing the 5' monophosphate of the mRNA body to 5' to 3' exoribonucleases. The activity of Dcp2 is stimulated by a variety of pathway specific co-activators through mechanisms that ..
  24. RNases and RNA Metabolism in Bacteria
    Murray P Deutscher; Fiscal Year: 2010
    ..of functional domains and mechanism of action of RNase R;4) Elucidation of the physiological role of exoribonucleases;and 5) Identification of RNases required for maturation of ribosomal RNA...
  25. RNases and RNA Metabolism in Bacteria
    MURRAY DEUTSCHER; Fiscal Year: 2009
    ..of functional domains and mechanism of action of RNase R; 4) Elucidation of the physiological role of exoribonucleases; and 5) Identification of RNases required for maturation of ribosomal RNA...
  26. ENZYMOLOGY AND CONTROL OF AMINO ACID ACTIVATION
    MURRAY DEUTSCHER; Fiscal Year: 2007
    ..of functional domains and mechanism of action of RNase R; 4) Elucidation of the physiological role of exoribonucleases; and 5) Identification of RNases required for maturation of ribosomal RNA...
  27. RNases and RNA Metabolism in Bacteria
    MURRAY DEUTSCHER; Fiscal Year: 2007
    ..of functional domains and mechanism of action of RNase R; 4) Elucidation of the physiological role of exoribonucleases; and 5) Identification of RNases required for maturation of ribosomal RNA...
  28. Comparative Analysis of RNA Helicases in E. coli
    Chaitanya Jain; Fiscal Year: 2009
    ..Advanced knowledge of such RNA helicases could also be helpful to design novel antibiotics that target conserved DEAD-box domains in pathogenic organisms. ..
  29. Comparative Analysis of RNA Helicases in E. coli
    Chaitanya Jain; Fiscal Year: 2010
    ..Advanced knowledge of such RNA helicases could also be helpful to design novel antibiotics that target conserved DEAD-box domains in pathogenic organisms. ..
  30. Comparative Analysis of RNA Helicases in E. coli
    Chaitanya Jain; Fiscal Year: 2010
    ..Advanced knowledge of such RNA helicases could also be helpful to design novel antibiotics that target conserved DEAD-box domains in pathogenic organisms. ..
  31. Regulation of APETALA2 in flower development in Arabidopsis
    Xuemei Chen; Fiscal Year: 2007
    ..This proposed research will undoubtedly provide insights into the integration of posttranscriptional and transcriptional mechanisms in developmental processes in multicellular organisms. ..
  32. Hec1 network as molecular therapeutic targets
    Wen Hwa Lee; Fiscal Year: 2007
    ..Moreover, the identified small molecules will not only be a useful tool for dissecting the mitotic machinery, but will have potentials for therapeutic application to treat cancer. ..
  33. Transcriptional Role of BRCA1 in Breast Cancer
    Wen Hwa Lee; Fiscal Year: 2009
    ..Especially, these natural factors that specifically suppress proliferation and induce breast cancer cell death will potentially provide a novel tumor dormancy therapy for treating breast cancer. ..
  34. NUCLEAR PATHWAY FOR MESSENGER RNA DEGRADATION IN YEAST
    James Butler; Fiscal Year: 2002
    ....