exoribonucleases

Summary

Summary: A family of enzymes that catalyze the exonucleolytic cleavage of RNA. It includes EC 3.1.13.-, EC 3.1.14.-, EC 3.1.15.-, and EC 3.1.16.-. EC 3.1.-

Top Publications

  1. ncbi The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases
    P Mitchell
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 91:457-66. 1997
  2. ncbi Cryptic pol II transcripts are degraded by a nuclear quality control pathway involving a new poly(A) polymerase
    Françoise Wyers
    Equipe labelisée La Ligue, Avenue de la Terrasse, 91190 Gif sur Yvette, Paris, France
    Cell 121:725-37. 2005
  3. ncbi GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets
    Joerg E Braun
    Department of Biochemistry, Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Mol Cell 44:120-33. 2011
  4. pmc The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosome
    Claudia Schneider
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Nucleic Acids Res 37:1127-40. 2009
  5. ncbi A single subunit, Dis3, is essentially responsible for yeast exosome core activity
    Andrzej Dziembowski
    Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique UPR2167, associée à l Université Pierre et Marie Curie, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Nat Struct Mol Biol 14:15-22. 2007
  6. ncbi The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II
    Minkyu Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:517-22. 2004
  7. pmc The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L
    Rafal Tomecki
    Department of Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    EMBO J 29:2342-57. 2010
  8. ncbi Endonucleolytic RNA cleavage by a eukaryotic exosome
    Alice Lebreton
    Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, CNRS UPR 2167, 91198 Gif sur Yvette, France
    Nature 456:993-6. 2008
  9. ncbi RNA degradation in Bacillus subtilis: an interplay of essential endo- and exoribonucleases
    Martin Lehnik-Habrink
    Department of General Microbiology, Georg August University Gottingen, Grisebachstr 8, D 37077 Gottingen, Germany
    Mol Microbiol 84:1005-17. 2012
  10. ncbi Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts
    Lidia Vasiljeva
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Mol Cell 21:239-48. 2006

Detail Information

Publications259 found, 100 shown here

  1. ncbi The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases
    P Mitchell
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 91:457-66. 1997
    ..Remarkably, four of these proteins are homologous to characterized bacterial 3'-->5' exoribonucleases; Rrp44p is homologous to RNase II, while Rrp41p, Rrp42p, and Rrp43p are related to RNase PH...
  2. ncbi Cryptic pol II transcripts are degraded by a nuclear quality control pathway involving a new poly(A) polymerase
    Françoise Wyers
    Equipe labelisée La Ligue, Avenue de la Terrasse, 91190 Gif sur Yvette, Paris, France
    Cell 121:725-37. 2005
    ..Our data strongly support the existence of a posttranscriptional quality control mechanism limiting inappropriate expression of genetic information...
  3. ncbi GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets
    Joerg E Braun
    Department of Biochemistry, Max Planck Institute for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    Mol Cell 44:120-33. 2011
    ....
  4. pmc The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosome
    Claudia Schneider
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Nucleic Acids Res 37:1127-40. 2009
    ..Finally, the N-terminal PIN domain was shown to be necessary and sufficient for association with the core exosome, indicating its dual function as a nuclease and structural element...
  5. ncbi A single subunit, Dis3, is essentially responsible for yeast exosome core activity
    Andrzej Dziembowski
    Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique UPR2167, associée à l Université Pierre et Marie Curie, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Nat Struct Mol Biol 14:15-22. 2007
    ..We observed that the yeast exosome ring mediates interactions with protein partners, providing an explanation for its essential function...
  6. ncbi The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II
    Minkyu Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:517-22. 2004
    ..These findings support a model in which poly(A) site cleavage and subsequent degradation of the 3'-downstream RNA by Rat1 trigger transcription termination...
  7. pmc The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L
    Rafal Tomecki
    Department of Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    EMBO J 29:2342-57. 2010
    ..Both hDIS3 and hDIS3L are active exonucleases; however, only hDIS3 has retained endonucleolytic activity. Our data suggest that three different ribonucleases can serve as catalytic subunits for the exosome in human cells...
  8. ncbi Endonucleolytic RNA cleavage by a eukaryotic exosome
    Alice Lebreton
    Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, CNRS UPR 2167, 91198 Gif sur Yvette, France
    Nature 456:993-6. 2008
    ..The mode of action of eukaryotic exosome cores in RNA processing and degradation should be reconsidered, taking into account the cooperation between its multiple ribonucleolytic activities...
  9. ncbi RNA degradation in Bacillus subtilis: an interplay of essential endo- and exoribonucleases
    Martin Lehnik-Habrink
    Department of General Microbiology, Georg August University Gottingen, Grisebachstr 8, D 37077 Gottingen, Germany
    Mol Microbiol 84:1005-17. 2012
    ..The implications of these findings for pathogenic Gram-positive bacteria are also discussed...
  10. ncbi Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts
    Lidia Vasiljeva
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Mol Cell 21:239-48. 2006
    ..Since Nrd1 is known to bind RNA polymerase II and be important for sn/snoRNA 3' end processing, Nrd1 may link transcription and RNA 3' end formation with surveillance by the exosome...
  11. pmc Yeast exosome mutants accumulate 3'-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs
    A van Hoof
    Department of Molecular Biology, Howard Hughes Medical Institute, University of Arizona, Tucson, Arizona 85721, USA
    Mol Cell Biol 20:441-52. 2000
    ..This finding suggests that the cytoplasmic and nuclear forms of the exosome represent two functionally different complexes involved in distinct 3'-to-5' processing and degradation reactions...
  12. ncbi A direct interaction between DCP1 and XRN1 couples mRNA decapping to 5' exonucleolytic degradation
    Joerg E Braun
    Department of Biochemistry, Max Planck Institute for Developmental Biology, Tubingen, Germany
    Nat Struct Mol Biol 19:1324-31. 2012
    ..Our findings reveal a role for XRN1 in decapping and provide a molecular basis for the coupling of decapping to 5'→3' mRNA degradation...
  13. pmc Pause sites promote transcriptional termination of mammalian RNA polymerase II
    Natalia Gromak
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Mol Cell Biol 26:3986-96. 2006
    ..Taken together, we provide new insights into the mechanism of pause site-mediated termination and establish a general role for the 5'-->3' exonuclease Xrn2 in Pol II termination...
  14. ncbi Active turnover modulates mature microRNA activity in Caenorhabditis elegans
    Saibal Chatterjee
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, CH 4002 Basel, Switzerland
    Nature 461:546-9. 2009
    ..This pathway might represent a potential target for therapeutic intervention on miRNA expression...
  15. pmc The exosome contains domains with specific endoribonuclease, exoribonuclease and cytoplasmic mRNA decay activities
    Daneen Schaeffer
    Department of Microbiology and Molecular Genetics, University of Texas Health Science Center Houston, 6431 Fannin Street, MSB 1 212 Houston, Texas 77030, USA
    Nat Struct Mol Biol 16:56-62. 2009
    ..The combination of an endoRNase and an exoRNase activity seems to be a widespread feature of RNA-degrading machines...
  16. pmc The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination
    Syuzo Kaneko
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Genes Dev 21:1779-89. 2007
    ..Together, our data indicate that p54nrb/PSF functions in recruitment of XRN2 to facilitate pre-mRNA 3' processing and transcription termination...
  17. pmc Discovery of the first insect nidovirus, a missing evolutionary link in the emergence of the largest RNA virus genomes
    Phan Thi Nga
    Department of Virology, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam
    PLoS Pathog 7:e1002215. 2011
    ..Together these characteristics identify NDiV as the prototype of a new nidovirus family and a missing link in the transition from small to large nidoviruses...
  18. pmc Human Pat1b connects deadenylation with mRNA decapping and controls the assembly of processing bodies
    Sevim Ozgur
    German Cancer Research Center, DKFZ ZMBH Alliance, Heidelberg, Germany
    Mol Cell Biol 30:4308-23. 2010
    ..Taken together, these findings provide evidence that human Pat1b is a central component of the RNA decay machinery by physically connecting deadenylation with decapping...
  19. ncbi 5'-to-3' exoribonuclease activity in bacteria: role of RNase J1 in rRNA maturation and 5' stability of mRNA
    Nathalie Mathy
    CNRS UPR 9073 affiliated with Université de Paris 7 Denis Diderot, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris
    Cell 129:681-92. 2007
    ..We show that a 30S ribosomal subunit bound to a Shine Dalgarno-like element (Stab-SD) in the cryIIIA mRNA blocks exonucleolytic progression of RNase J1, accounting for the stabilizing effect of this element in vivo...
  20. ncbi MicroRNA assassins: factors that regulate the disappearance of miRNAs
    Zoya S Kai
    Department of Biology, University of California, San Diego, La Jolla, California, USA
    Nat Struct Mol Biol 17:5-10. 2010
    ..The discovery of specific cis-acting modifications and trans-acting proteins that affect miRNA half-life reveals new elements that contribute to the homeostasis of these vital regulatory molecules...
  21. ncbi Rrp6p, the yeast homologue of the human PM-Scl 100-kDa autoantigen, is essential for efficient 5.8 S rRNA 3' end formation
    M W Briggs
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14618, USA
    J Biol Chem 273:13255-63. 1998
    ..These findings indicate that Rrp6p plays a role in 5.8 S rRNA 3' end formation, and they identify a functional intermediate in the rRNA processing pathway...
  22. ncbi Identification of a regulated pathway for nuclear pre-mRNA turnover
    C Bousquet-Antonelli
    Wellcome Trust Centre for Cell Biology ICMB, University of Edinburgh, Scotland
    Cell 102:765-75. 2000
    ..We propose that nuclear pre-mRNA turnover represents a novel step in the regulation of gene expression...
  23. ncbi Human 5' --> 3' exonuclease Xrn2 promotes transcription termination at co-transcriptional cleavage sites
    Steven West
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Nature 432:522-5. 2004
    ..Degradation of the downstream cleavage product by Xrn2 results in transcriptional termination, as envisaged in the torpedo model...
  24. ncbi Genome-wide analysis of mRNAs regulated by the nonsense-mediated and 5' to 3' mRNA decay pathways in yeast
    Feng He
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Mol Cell 12:1439-52. 2003
    ....
  25. pmc Dis3-like 1: a novel exoribonuclease associated with the human exosome
    Raymond H J Staals
    Department of Biomolecular Chemistry, Nijmegen Center for Molecular Life Sciences, Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, The Netherlands
    EMBO J 29:2358-67. 2010
    ..Taken together, these data indicate that hDis3L1 is a novel exosome-associated exoribonuclease in the cytoplasm of human cells...
  26. pmc Polyadenylation linked to transcription termination directs the processing of snoRNA precursors in yeast
    Pawel Grzechnik
    Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawinskiego 5A, 02 106 Warsaw, Poland
    Mol Cell 32:247-58. 2008
    ..We propose a model in which polyadenylation of pre-snoRNAs is a key event linking their transcription termination, 3' end processing, and degradation...
  27. pmc Assembly of Saccharomyces cerevisiae 60S ribosomal subunits: role of factors required for 27S pre-rRNA processing
    Aarti Sahasranaman
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA
    EMBO J 30:4020-32. 2011
    ..In the absence of these assembly factors, at least one exonuclease can switch from processing to turnover of pre-rRNA...
  28. pmc Structure of the Lassa virus nucleoprotein reveals a dsRNA-specific 3' to 5' exonuclease activity essential for immune suppression
    Kathryn M Hastie
    Departments of Immunology and Microbial Science and Molecular Biology, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 108:2396-401. 2011
    ..Thus, the nucleoprotein is a viral exonuclease with anti-immune activity, and this work provides a unique opportunity to combat arenaviral infections...
  29. pmc Cap binding and immune evasion revealed by Lassa nucleoprotein structure
    Xiaoxuan Qi
    Biomedical Sciences Research Complex, School of Chemistry, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK
    Nature 468:779-83. 2010
    ..These findings provide great potential for vaccine and drug development...
  30. pmc The roles of 3'-exoribonucleases and the exosome in trypanosome mRNA degradation
    Abeer Fadda
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Alliance, D69120 Heidelberg, Germany
    RNA 19:937-47. 2013
    ..Results suggested that the exosome is involved in the processing of trypanosome snoRNAs. There was no correlation between effects on half-lives and on mRNA abundance...
  31. pmc Polyadenylation helps regulate functional tRNA levels in Escherichia coli
    Bijoy K Mohanty
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Nucleic Acids Res 40:4589-603. 2012
    ..Surprisingly, neither PNPase nor RNase II has any effect on tRNA poly(A) tail length. Our data suggest that the polyadenylation of tRNAs by PAP I likely proceeds in a distributive fashion unlike what is observed with mRNAs...
  32. ncbi Molecular mechanisms of aging-associated inflammation
    Devanand Sarkar
    Department of Pathology, Herbert Irving Comprehensive Cancer Center, College of Physicians and Surgeons, Columbia University Medical Center, New York, NY 10032, USA
    Cancer Lett 236:13-23. 2006
    ..In these contexts, inhibition of hPNPase(old-35) may represent a novel molecular target for intervening in aging-associated chronic diseases...
  33. pmc The yeast exosome and human PM-Scl are related complexes of 3' --> 5' exonucleases
    C Allmang
    Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR UK
    Genes Dev 13:2148-58. 1999
    We previously identified a complex of 3' --> 5' exoribonucleases, designated the exosome, that is expected to play a major role in diverse RNA processing and degradation pathways...
  34. pmc A live, impaired-fidelity coronavirus vaccine protects in an aged, immunocompromised mouse model of lethal disease
    Rachel L Graham
    Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Nat Med 18:1820-6. 2012
    ..ExoN inactivation has potential for broad applications in the stable attenuation of CoVs and, perhaps, other RNA viruses...
  35. pmc Structural and biochemical studies of the 5'→3' exoribonuclease Xrn1
    Jeong Ho Chang
    Department of Biological Sciences, Columbia University, New York, NY, USA
    Nat Struct Mol Biol 18:270-6. 2011
    The 5'→3' exoribonucleases (XRNs) have important functions in transcription, RNA metabolism and RNA interference...
  36. ncbi Concerted action of poly(A) nucleases and decapping enzyme in mammalian mRNA turnover
    Akio Yamashita
    Department of Biochemistry and Molecular Biology, The University of Texas Medical School, Houston, Texas 77030, USA
    Nat Struct Mol Biol 12:1054-63. 2005
    ..Our findings reveal a functional link between deadenylation and decapping and help to define in vivo pathways for mammalian cytoplasmic mRNA decay...
  37. pmc C1D and hMtr4p associate with the human exosome subunit PM/Scl-100 and are involved in pre-rRNA processing
    Geurt Schilders
    Department of Biomolecular Chemistry, Nijmegen Center for Molecular Life Sciences, Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, The Netherlands
    Nucleic Acids Res 35:2564-72. 2007
    The exosome is a complex of 3'-5' exoribonucleases and RNA-binding proteins, which is involved in processing or degradation of different classes of RNA...
  38. ncbi AtXRN4 degrades mRNA in Arabidopsis and its substrates include selected miRNA targets
    Frederic F Souret
    Delaware Biotechnology Institute, University of Delaware, Newark 19711, USA
    Mol Cell 15:173-83. 2004
    ..The demonstration that an Xrn1p homolog degrades mRNA in a multicellular eukaryote and contributes to the miRNA-mediated decay pathway of selected targets has implications for XRNs in other organisms...
  39. ncbi Structure of the active subunit of the yeast exosome core, Rrp44: diverse modes of substrate recruitment in the RNase II nuclease family
    Esben Lorentzen
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Mol Cell 29:717-28. 2008
    ..The structure provides a molecular rationale for the observed biochemical properties of the RNase R family of nucleases...
  40. ncbi A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans
    Scott Kennedy
    Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 427:645-9. 2004
    ..Thus, ERI-1 is a negative regulator that may normally function to limit the duration, cell-type specificity or endogenous functions of RNA interference...
  41. pmc The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels
    K F Jensen
    Institute of Biological Chemistry, University of Copenhagen, Denmark
    J Bacteriol 175:3401-7. 1993
    ....
  42. ncbi Distinct pathways for snoRNA and mRNA termination
    Minkyu Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 24:723-34. 2006
    ..These results suggest that in yeast the transcribing RNA polymerase II can choose between two distinct termination mechanisms but keeps both options available during elongation...
  43. pmc Acetylation regulates the stability of a bacterial protein: growth stage-dependent modification of RNase R
    Wenxing Liang
    Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33101, USA
    Mol Cell 44:160-6. 2011
    ..These findings indicate that acetylation can regulate the stability of a bacterial protein...
  44. pmc The Ccr4a (CNOT6) and Ccr4b (CNOT6L) deadenylase subunits of the human Ccr4-Not complex contribute to the prevention of cell death and senescence
    Saloni Mittal
    School of Pharmacy and Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, United Kingdom
    Mol Biol Cell 22:748-58. 2011
    ....
  45. ncbi Unravelling the dynamics of RNA degradation by ribonuclease II and its RNA-bound complex
    Carlos Frazao
    Division of Biological Chemistry, ITQB Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apt 127, 2781 901 Oeiras, Portugal
    Nature 443:110-4. 2006
    ..Moreover, it reveals important structural features that can be extrapolated to other members of this family...
  46. pmc Budding yeast RNA polymerases I and II employ parallel mechanisms of transcriptional termination
    Junya Kawauchi
    Sir William Dunn School of Pathology, Oxford OX1 3RE, United Kingdom
    Genes Dev 22:1082-92. 2008
    ....
  47. pmc Structure and function of the 5'-->3' exoribonuclease Rat1 and its activating partner Rai1
    Song Xiang
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Nature 458:784-8. 2009
    The 5'-->3' exoribonucleases (XRNs) comprise a large family of conserved enzymes in eukaryotes with crucial functions in RNA metabolism and RNA interference...
  48. ncbi Reconstitution, activities, and structure of the eukaryotic RNA exosome
    Quansheng Liu
    Structural Biology Program, Sloan Kettering Institute, New York, NY 10021, USA
    Cell 127:1223-37. 2006
    ..To elucidate the architecture of a eukaryotic exosome, its conserved surfaces, and the structural basis for RNA decay, we report the X-ray structure determination for the 286 kDa nine-subunit human exosome at 3.35 A...
  49. ncbi The exosome subunit Rrp44 plays a direct role in RNA substrate recognition
    Claudia Schneider
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK
    Mol Cell 27:324-31. 2007
    ..Recognition of hypomodified tRNA(i)(Met) by Rrp44 is genetically separable from its catalytic activity on other substrates, with the mutations mapping to distinct regions of the protein...
  50. pmc Genome-wide mRNA surveillance is coupled to mRNA export
    Haley Hieronymus
    Department of Systems Biology, Harvard Medical School and the Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Genes Dev 18:2652-62. 2004
    ..These results demonstrate coupling of mRNA surveillance to mRNA export and suggest specificity of the RNA surveillance machinery for different transcript populations. Broadly, these findings link DNA and RNA surveillance to mRNA export...
  51. ncbi Deadenylation of the unstable mRNA encoded by the yeast MFA2 gene leads to decapping followed by 5'-->3' digestion of the transcript
    D Muhlrad
    Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721
    Genes Dev 8:855-66. 1994
    ..Because the poly(A) tail and the cap structure are found on essentially all mRNAs, this pathway could be a general mechanism for the decay of many eukaryotic transcripts...
  52. ncbi The archaeal exosome core is a hexameric ring structure with three catalytic subunits
    Esben Lorentzen
    European Molecular Biology Laboratory, Heidelberg, Germany
    Nat Struct Mol Biol 12:575-81. 2005
    ..of three Rrp41-Rrp42 heterodimers, where both subunits adopt the RNase PH fold common to phosphorolytic exoribonucleases. Structure-guided mutagenesis reveals that the activity of the complex resides within the active sites of the ..
  53. ncbi Human Ccr4-Not complexes contain variable deadenylase subunits
    Nga Chi Lau
    Department of Physiological Chemistry, University Medical Center Utrecht, Utrecht, The Netherlands
    Biochem J 422:443-53. 2009
    ..Taken together, human Ccr4-Not complexes are heterogeneous in composition owing to differences in their deadenylase subunits, which may reflect the multi-functionality of these complexes in cellular processes...
  54. ncbi The Sm-like protein Hfq regulates polyadenylation dependent mRNA decay in Escherichia coli
    Bijoy K Mohanty
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Mol Microbiol 54:905-20. 2004
    ..Analysis of mRNA half-lives in hfq, deltapcnB and hfq deltapcnB mutants suggests that Hfq and PAP I function in the same mRNA decay pathway...
  55. pmc Interactions between mRNA export commitment, 3'-end quality control, and nuclear degradation
    Domenico Libri
    Centre National de la Recherche Scientifique, Centre de Genetique Moleculaire, 91190 Gif sur Yvette, France
    Mol Cell Biol 22:8254-66. 2002
    ....
  56. pmc A nuclear 3'-5' exonuclease involved in mRNA degradation interacts with Poly(A) polymerase and the hnRNA protein Npl3p
    K T Burkard
    Department of Microbiology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14618, USA
    Mol Cell Biol 20:604-16. 2000
    ..These findings suggest that Rrp6p may interact with the mRNA polyadenylation system and thereby play a role in a nuclear pathway for the degradation of aberrantly processed precursor mRNAs...
  57. pmc RNA polyadenylation and degradation in different Archaea; roles of the exosome and RNase R
    Victoria Portnoy
    Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Nucleic Acids Res 34:5923-31. 2006
    ..This ribonuclease is not able to degrade structured RNA better than PNPase. RNase R, which appears to be the only exoribonucleases in Haloferax volcanii, was found to be essential for viability.
  58. pmc Identification of a quality-control mechanism for mRNA 5'-end capping
    Xinfu Jiao
    Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854, USA
    Nature 467:608-11. 2010
    ..We propose that Rai1 is involved in an as yet uncharacterized quality control process that ensures mRNA 5'-end integrity by an aberrant-cap-mediated mRNA decay mechanism...
  59. pmc Efficient termination of transcription by RNA polymerase I requires the 5' exonuclease Rat1 in yeast
    Aziz El Hage
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
    Genes Dev 22:1069-81. 2008
    ..When Rat1 catches Pol I, which is predicted to be paused at T1, transcription is terminated...
  60. ncbi Yeast RNase III triggers polyadenylation-independent transcription termination
    Ghada Ghazal
    RNA Group, Departement de Microbiologie et d Infectiologie, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
    Mol Cell 36:99-109. 2009
    ..These results support a model for transcription termination in which cotranscriptional cleavage by Rnt1p provides access for exoribonucleases in the absence of polyadenylation signals.
  61. pmc Post-translational modification of RNase R is regulated by stress-dependent reduction in the acetylating enzyme Pka (YfiQ)
    Wenxing Liang
    Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, Florida 33101, USA
    RNA 18:37-41. 2012
    ..We find that RNase R in cold-shocked cells also lacks the acetyl modification due to the absence of Pka. These data indicate that RNase R stability depends on Pka, which itself is regulated under stress conditions...
  62. pmc Archaeal β-CASP ribonucleases of the aCPSF1 family are orthologs of the eukaryal CPSF-73 factor
    Duy Khanh Phung
    Centre National de la Recherche Scientifique, LMGM and Université de Toulouse, UPS, F 31062 Toulouse, France
    Nucleic Acids Res 41:1091-103. 2013
    ..Altogether, our results suggest that aCPSF1 performs an essential function and that an enzyme with similar activities was present in the last common ancestor of Archaea and Eukarya...
  63. pmc The nuclear exosome contributes to autogenous control of NAB2 mRNA levels
    Kelly M Roth
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Box 672, 601 Elmwood Ave, Rochester, NY 14642, USA
    Mol Cell Biol 25:1577-85. 2005
    ..These findings suggest a novel regulatory circuit in which the nuclear exosome controls the level of NAB2 mRNA in response to changes in the activity of Nab2 protein...
  64. pmc RNA 3'-end mismatch excision by the severe acute respiratory syndrome coronavirus nonstructural protein nsp10/nsp14 exoribonuclease complex
    Mickaël Bouvet
    Architecture et Fonction des Macromolecules Biologiques, Unité Mixte de Recherche 7257, Ecole Supérieure d Ingénieurs de Luminy Case 925, Centre National de la Recherche Scientifique and Aix Marseille Université, 13288 Marseille, France
    Proc Natl Acad Sci U S A 109:9372-7. 2012
    ..In addition to the activation of nsp16-mediated 2'-O-MTase activity, nsp10 also activates nsp14 in an RNA processing function potentially connected to a replicative mismatch repair mechanism...
  65. pmc Exoribonuclease superfamilies: structural analysis and phylogenetic distribution
    Y Zuo
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, PO Box 016129, Miami, FL 33101 6129, USA
    Nucleic Acids Res 29:1017-26. 2001
    b>Exoribonucleases play an important role in all aspects of RNA metabolism. Biochemical and genetic analyses in recent years have identified many new RNases and it is now clear that a single cell can contain multiple enzymes of this class...
  66. pmc A nuclear surveillance pathway for mRNAs with defective polyadenylation
    Laura Milligan
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
    Mol Cell Biol 25:9996-10004. 2005
    ..In wild-type strains, assembly of the cleavage and polyadenylation complex might be suboptimal at cryptic polyadenylation sites, causing slowed polyadenylation...
  67. pmc Maturation of mammalian H/ACA box snoRNAs: PAPD5-dependent adenylation and PARN-dependent trimming
    Heike Berndt
    Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, 06099 Halle, Germany
    RNA 18:958-72. 2012
    ..The noncanonical poly(A) polymerase PAPD5 is responsible for addition of the oligo(A) tails. We suggest that deadenylation is coupled to clean 3' end trimming, which might serve to enhance snoRNA stability...
  68. pmc Reconstitution of an Argonaute-dependent small RNA biogenesis pathway reveals a handover mechanism involving the RNA exosome and the exonuclease QIP
    Zhihong Xue
    Department of Physiology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Mol Cell 46:299-310. 2012
    ..In addition, exosome is also important for the decay of sRNAs. Together, our results establish a biochemical mechanism of an Argonaute-dependent sRNA biogenesis pathway and critical roles of exosome in sRNA processing...
  69. pmc Quantitative analysis of processive RNA degradation by the archaeal RNA exosome
    Sophia Hartung
    Center for Integrated Protein Sciences, Department of Biochemistry, Ludwig Maximilians University Munich, Munich, Germany
    Nucleic Acids Res 38:5166-76. 2010
    ....
  70. pmc Infidelity of SARS-CoV Nsp14-exonuclease mutant virus replication is revealed by complete genome sequencing
    Lance D Eckerle
    Department of Pediatrics and Microbiology and Immunology and Elizabeth B Lamb Center for Pediatric Research, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    PLoS Pathog 6:e1000896. 2010
    ....
  71. ncbi The role of 3'-5' exoribonucleases in RNA degradation
    José M Andrade
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Qeiras, Portugal
    Prog Mol Biol Transl Sci 85:187-229. 2009
    ..Escherichia coli has eight 3'-5' exoribonucleases, which are involved in multiple RNA metabolic pathways...
  72. ncbi RNase II levels change according to the growth conditions: characterization of gmr, a new Escherichia coli gene involved in the modulation of RNase II
    F Cairrao
    Instituto Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apart 127, 2781 901 Oeiras, Portugal
    Mol Microbiol 39:1550-61. 2001
    ..The data presented here show that the regulation of ribonucleolytic activity can depend on growth conditions, and this regulation can be mediated by factors that are not RNases...
  73. pmc Human mitochondrial SUV3 and polynucleotide phosphorylase form a 330-kDa heteropentamer to cooperatively degrade double-stranded RNA with a 3'-to-5' directionality
    Dennis Ding Hwa Wang
    Department of Biological Chemistry, University of California, Irvine, California 92697, USA
    J Biol Chem 284:20812-21. 2009
    ..Taken together, these results strongly suggest that the complex of hSUV3-hPNPase is an integral entity for efficient degradation of structured RNA and may be the long sought RNA-degrading complex in the mammalian mitochondria...
  74. pmc Coupled 5' nucleotide recognition and processivity in Xrn1-mediated mRNA decay
    Martin Jinek
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 41:600-8. 2011
    ....
  75. ncbi Identification of 2'-phosphodiesterase, which plays a role in the 2-5A system regulated by interferon
    Kazuishi Kubota
    Biomedical Research Laboratories, Sankyo Co, Ltd, 1 2 58, Hiromachi, Shinagawa ku, Tokyo 140 8710, Japan
    J Biol Chem 279:37832-41. 2004
    ..These observations identify 2'-PDE as a key regulator of the 2-5A system and as a potential novel target for antiviral and antitumor treatments...
  76. ncbi Facilitation of mRNA deadenylation and decay by the exosome-bound, DExH protein RHAU
    Hoanh Tran
    Friedrich Miescher Institute for Biomedical Research, Novartis Research Foundation, Maulbeerstrasse 66, CH 4058 Basel, Switzerland
    Mol Cell 13:101-11. 2004
    ..that either stabilize or otherwise degrade ARE-mRNAs by recruiting the exosome, a complex of 3'-to-5' exoribonucleases. We have identified RHAU, a putative DExH RNA helicase that was isolated in association with the ARE of ..
  77. pmc RNase MRP cleaves the CLB2 mRNA to promote cell cycle progression: novel method of mRNA degradation
    Tina Gill
    Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA
    Mol Cell Biol 24:945-53. 2004
    ..In addition, these results denote a new mechanism of mRNA degradation not seen before in the yeast Saccharomyces cerevisiae...
  78. pmc Lithium toxicity in yeast is due to the inhibition of RNA processing enzymes
    B Dichtl
    EMBL, Gene Expression Programme, Postfach 10 2209, 69012 Heidelberg, Germany
    EMBO J 16:7184-95. 1997
    ..Here we report that pAp accumulation in HAL2 mutants inhibits the 5'-->3' exoribonucleases Xrn1p and Rat1p...
  79. pmc Quality control of ribosomal RNA mediated by polynucleotide phosphorylase and RNase R
    Zhuan Fen Cheng
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, FL 33101, USA
    Proc Natl Acad Sci U S A 100:6388-93. 2003
    ..In the absence of these RNases, rRNA fragments accumulate, leading to interference with ribosome maturation and ultimately to cell death...
  80. pmc The 1.4-A crystal structure of the S. pombe Pop2p deadenylase subunit unveils the configuration of an active enzyme
    Anette Thyssen Jonstrup
    Centre for Structural Biology, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Arhus C, Denmark
    Nucleic Acids Res 35:3153-64. 2007
    ....
  81. pmc 5'-end surveillance by Xrn2 acts as a shared mechanism for mammalian pre-rRNA maturation and decay
    Minshi Wang
    Department of Cell Biology, University of Medicine and Dentistry of New Jersey, Stratford, NJ 08084, USA
    Nucleic Acids Res 39:1811-22. 2011
    ..We propose that probing of pre-rRNA maturation intermediates by exonucleases serves the dual function of generating mature rRNAs and suppressing suboptimal processing paths during ribosome assembly...
  82. ncbi The yeast 5'-3' exonuclease Rat1p functions during transcription elongation by RNA polymerase II
    Silvia Jimeno-González
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, Aarhus University, Aarhus DK 8000, Denmark
    Mol Cell 37:580-7. 2010
    ..Our data suggest that Rat1p serves to terminate RNAPII molecules engaged in the production of uncapped RNA, regardless of their position on the gene locus...
  83. pmc The 5' external transcribed spacer in mouse ribosomal RNA contains two cleavage sites
    Tatyana Kent
    Department of Cell Biology, School of Osteopathic Medicine, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    RNA 15:14-20. 2009
    ..We discuss the finding of a second processing site in mammalian 5'ETS in relation to the involvement of the U3 snoRNA in pre-rRNA processing and present a revised map of the mouse 18S rRNA processing pathway...
  84. ncbi Decay of endoplasmic reticulum-localized mRNAs during the unfolded protein response
    Julie Hollien
    Department of Cellular and Molecular Pharmacology, University of California San Francisco, Howard Hughes Medical Institute, San Francisco, CA 94143, USA
    Science 313:104-7. 2006
    ..This response is well suited to complement other UPR mechanisms because it could selectively halt production of proteins that challenge the ER and clear the translocation and folding machinery for the subsequent remodeling process...
  85. pmc Transcription termination by nuclear RNA polymerases
    Patricia Richard
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Genes Dev 23:1247-69. 2009
    ..In this review, we describe the present state of knowledge on how each of the three RNA polymerases terminates and how mechanisms are conserved, or vary, from yeast to human...
  86. ncbi Genome-wide high-resolution mapping of exosome substrates reveals hidden features in the Arabidopsis transcriptome
    Julia A Chekanova
    School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110, USA
    Cell 131:1340-53. 2007
    ..These first genome-wide maps of exosome substrates will aid in illuminating new fundamental components and regulatory mechanisms of eukaryotic transcriptomes...
  87. ncbi Antisense RNA stabilization induces transcriptional gene silencing via histone deacetylation in S. cerevisiae
    Jurgi Camblong
    Department of Cell Biology, Sciences III, University of Geneva, 30 quai E Ansermet, 1211 Geneva 4, Switzerland
    Cell 131:706-17. 2007
    ....
  88. ncbi Polynucleotide phosphorylase, RNase II and RNase E play different roles in the in vivo modulation of polyadenylation in Escherichia coli
    B K Mohanty
    Department of Genetics, University of Georgia, Athens, GA 30605, USA
    Mol Microbiol 36:982-94. 2000
    ..Conversely, toxicity is significantly reduced in the presence of excess RNase II. Overproduction of RNase E leads to increased polyadenylation and no reduction in toxicity...
  89. pmc Bacillus subtilis YhaM, a member of a new family of 3'-to-5' exonucleases in gram-positive bacteria
    Irina A Oussenko
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine of New York University, New York 10029, USA
    J Bacteriol 184:6250-9. 2002
    A strain of Bacillus subtilis lacking two 3'-to-5' exoribonucleases, polynucleotide phosphorylase (PNPase) and RNase R, was used to purify another 3'-to-5' exoribonuclease, which is encoded by the yhaM gene...
  90. pmc Degradation of microRNAs by a family of exoribonucleases in Arabidopsis
    Vanitharani Ramachandran
    Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California Riverside, Riverside, CA 92521, USA
    Science 321:1490-2. 2008
    ..Here, we show that a family of exoribonucleases encoded by the SMALL RNA DEGRADING NUCLEASE (SDN) genes degrades mature miRNAs in Arabidopsis...
  91. pmc Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1
    Irina Chernyakov
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
    Genes Dev 22:1369-80. 2008
    ..The RTD pathway is the first to be implicated in the turnover of mature RNA species from the class of stable RNAs. These results and the results of others demonstrate that tRNA, like mRNA, is subject to multiple quality control steps...
  92. ncbi Nuclear retention of unspliced mRNAs in yeast is mediated by perinuclear Mlp1
    Vincent Galy
    Unité de Biologie Cellulaire du Noyau, CNRS URA 2582, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Cell 116:63-73. 2004
    ..We propose that at this confined site the perinuclear Mlp1 implements a quality control step prior to export, physically retaining faulty pre-mRNAs...
  93. ncbi Inhibition of human poly(A)-specific ribonuclease (PARN) by purine nucleotides: kinetic analysis
    Nikolaos A A Balatsos
    Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece
    J Enzyme Inhib Med Chem 24:516-23. 2009
    ..Our data imply that PARN activity can be modulated by purine nucleotides in vitro, providing an additional simple regulatory mechanism...
  94. pmc Novel features of the XRN-family in Arabidopsis: evidence that AtXRN4, one of several orthologs of nuclear Xrn2p/Rat1p, functions in the cytoplasm
    J P Kastenmayer
    Michigan State University, Department of Energy Plant Research Laboratory, and Biochemistry Department, 322 Plant Biology Building, East Lansing, MI 48824 1312, USA
    Proc Natl Acad Sci U S A 97:13985-90. 2000
    The 5'-3' exoribonucleases Xrn1p and Xrn2p/Rat1p function in the degradation and processing of several classes of RNA in Saccharomyces cerevisiae...
  95. pmc Exoribonuclease R in Mycoplasma genitalium can carry out both RNA processing and degradative functions and is sensitive to RNA ribose methylation
    Maureen S Lalonde
    Department of Biomedical Science, Florida Atlantic University, Boca Raton, FL 33431, USA
    RNA 13:1957-68. 2007
    ..In contrast, EcR degrades this pre-tRNA without the formation of specific products. Our results suggest that MgR shares some properties of both EcR and EcII and can carry out a broad range of RNA processing and degradative functions...
  96. pmc Exonucleolysis is required for nuclear mRNA quality control in yeast THO mutants
    Jannie Assenholt
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, The Faculty of Science, Aarhus University, DK 8000 Aarhus C, Denmark
    RNA 14:2305-13. 2008
    ..Our data show that exonucleolytic attack by the nuclear exosome is needed both for provoking mRNP QC and for its ensuing elimination of faulty RNA...
  97. pmc Serum-deprivation stimulates cap-binding by PARN at the expense of eIF4E, consistent with the observed decrease in mRNA stability
    Ruth Seal
    Department of Neurology, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK
    Nucleic Acids Res 33:376-87. 2005
    ..These phosphorylation-induced changes in the interplay of PARN and eIF4E may determine whether the mRNA is translated or decayed...
  98. ncbi Structural basis for processivity and single-strand specificity of RNase II
    Yuhong Zuo
    Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, P O Box 016129, Miami, FL 33101, USA
    Mol Cell 24:149-56. 2006
    RNase II is a member of the widely distributed RNR family of exoribonucleases, which are highly processive 3'-->5' hydrolytic enzymes that play an important role in mRNA decay. Here, we report the crystal structure of E...
  99. ncbi RNase II: the finer details of the Modus operandi of a molecular killer
    Cecilia M Arraiano
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    RNA Biol 7:276-81. 2010
    ..These exoribonucleases processively degrade RNA from the 3'-end releasing 5'-nucleotide monophosphates...
  100. ncbi Biochemical characterization of the RNase II family of exoribonucleases from the human pathogens Salmonella typhimurium and Streptococcus pneumoniae
    Susana Domingues
    Instituto de Tecnologia Quimica e Biologica Universidade Nova de Lisboa, Apartado 127, 2781 901 Oeiras, Portugal
    Biochemistry 48:11848-57. 2009
    ..Since ribonucleases have not been previously characterized in S. pneumoniae or Sa. typhimurium, this work provides an important first step in the understanding of post-transcriptional control in these pathogens...
  101. pmc Targeting of the cytosolic poly(A) binding protein PABPC1 to mitochondria causes mitochondrial translation inhibition
    Mateusz Wydro
    Mitochondrial Research Group, Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    Nucleic Acids Res 38:3732-42. 2010
    ..These data are consistent with endogenous RNA-binding factor(s) interacting with the poly(A) to optimize mitochondrial protein synthesis...

Research Grants22

  1. Regulation of an Iron Import Facilitator by Transcriptional Interference
    CAMILLE REYES NERY; Fiscal Year: 2010
    ..in cells lacking yeast RNase III and the presence of a 5'extended species in cells deficient in the exoribonucleases Xrn1 and Rat1. The 5'end of these extended species were found to originate ~3...
  2. Identification of New Antigens for a Plague Vaccine
    Ashok K Chopra; Fiscal Year: 2013
    ..We inferred from these data that other stress-associated genes (e.g., exoribonucleases) could also be involved in lpp-mediated, attenuated virulence of the bacterium...
  3. Global analysis of mRNA decay in Bacillus subtilis
    David H Bechhofer; Fiscal Year: 2013
    ..Earlier work identified two major 3'exoribonucleases in B...
  4. Structure and function of 5'to 3'exoribonucleases
    Liang Tong; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The 5'->3'exoribonucleases (XRNs) comprise a large family of conserved enzymes in eukaryotes with important functions in RNA metabolism and RNA interference...
  5. Fundamental Aspects of Kinetoplastid Mitochondrial RNA Stability
    Sara Lana Zimmer; Fiscal Year: 2013
    ..Attempts to identify and describe the regulation of cis elements recognized by exoribonucleases have met with limited success in T...
  6. Cis- and trans-acting factors in mitochondrial RNA decay
    Laurie K Read; Fiscal Year: 2012
    ..Two candidate exoribonucleases were identified in the T. brucei genome, and one was shown to be essential for optimal growth...
  7. Monitoring mechanisms in mammalian ribosome biogenesis
    Dimitri G Pestov; Fiscal Year: 2013
    ..Here, we wish to establish the pathway through which exoribonucleases start the process of elimination of the defective preribosomes. Our project has three specific aims. 1...
  8. Structure and Function of the Decapping Enzyme Complex
    John D Gross; Fiscal Year: 2013
    ..sentences an mRNA for destruction by exposing the 5'monophosphate of the mRNA body to 5'to 3' exoribonucleases. The activity of Dcp2 is stimulated by a variety of pathway specific co-activators through mechanisms that ..
  9. Compartmentalized Exosome Structure and Function
    Erik D Andrulis; Fiscal Year: 2010
    ..RNA metabolic pathways is the exosome, an essential protein complex comprising numerous 3'to 5'exoribonucleases. Yet, the exact mechanisms underlying how the exosome functions in these pathways are unclear...
  10. MITOCHONDRIAL RNA METABOLISM IN TRYPANOSOMA BRUCEI
    LAURIE READ; Fiscal Year: 2004
    ..We will also focus on identification and isolation of exoribonucleases. Finally, we will use genetic and biochemical means to isolate T...
  11. MITOCHONDRIAL RNA METABOLISM IN TRYPANOSOMA BRUCEI
    LAURIE READ; Fiscal Year: 2007
    ..We will identify poly(A)-selective exoribonucleases and characterize their function using RNAi-mediated targeted depletion...
  12. Structural studies of exoribonucleases
    Arun Malhotra; Fiscal Year: 2007
    ..as a model system for understanding the role of ribonucleases in cellular RNA metabolism, and eight distinct exoribonucleases have been identified in this bacterium...
  13. INITIATION OF MRNA DECAY IN BACILLUS SUBTILIS
    David H Bechhofer; Fiscal Year: 2010
    ..The availability of B. subtilis strains that are deficient in 1 or more 3'-to-5'exoribonucleases will make it possible to examine the role of individual ribonucleases in mRNA turnover...
  14. Biogenesis of Small RNAs
    SANDRA WOLIN; Fiscal Year: 2009
    ..By understanding these processes, it may be possible to design treatments for diseases caused by noncoding RNAs that do not function properly. ..
  15. RNA-Mediated Silencing: Mechanisms and Biological Roles in Chlamydomonas
    Heriberto Cerutti; Fiscal Year: 2009
    ..The overall findings are expected to improve our ability to exploit RNAi as an experimental and/or therapeutic tool, with likely impacts in both medicine and agriculture. ..
  16. Antibodies to Ro ribonucleoproteins as biomarkers in Sjogren's syndrome
    SANDRA WOLIN; Fiscal Year: 2007
    ..Our goal is to identify whether Sjogren's syndrome patients produce autoantibodies that will be useful for diagnosis and for predicting the course of the disease in individual patients. [unreadable] [unreadable] [unreadable]..
  17. GM - CSF - producing tumor cell line as a CML vaccine
    Catherine Wu; Fiscal Year: 2006
    ..The goal of this proposal is to carry out an initial phase I trial using this potentially valuable reagent and thus provide a basis for the continuation and refinement of clinical trials using this reagent. ..
  18. LEUKEMIA ANTIGENS FOLLOWING DONOR LEUKOCYTE INFUSION
    Catherine Wu; Fiscal Year: 2004
    ..abstract_text> ..
  19. CANCER SUPPRESSION BY THE RETINOBLASTOMA GENE
    Wen Hwa Lee; Fiscal Year: 2002
    ..Thus, firm grounds for clinical trials on a significant subset of advanced human cancers will be established. ..
  20. The Yeast Exosome as a Model Anticancer Drug Target
    James Butler; Fiscal Year: 2004
    ..8S rRNA processing. These experiments should clarify the connection between 5FU and rRNA processing and therefore provide a new pathway to which anticancer drugs can be targeted. ..
  21. COORDINATION OF BACTERIAL RIBOSOMAL PROTEIN SYNTHESIS
    Lasse Lindahl; Fiscal Year: 2001
    ....
  22. NUCLEAR PATHWAY FOR MESSENGER RNA DEGRADATION IN YEAST
    James Butler; Fiscal Year: 2002
    ....