calf thymus ribonuclease h

Summary

Summary: A ribonuclease that specifically cleaves the RNA moiety of RNA:DNA hybrids. It has been isolated from a wide variety of prokaryotic and eukaryotic organisms (particularly calf thymus) as well as retroviruses. EC 3.1.26.4.

Top Publications

  1. pmc Mutations of the RNase H C helix of the Moloney murine leukemia virus reverse transcriptase reveal defects in polypurine tract recognition
    David Lim
    Integrated Program in Cellular, Molecular and Biophysical Studies, Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Virol 76:8360-73. 2002
  2. ncbi Structural requirements at the catalytic site of the heteroduplex substrate for human RNase H1 catalysis
    Walt F Lima
    Department of Molecular and Structural Biology, Isis Pharmaceuticals, 2292 Faraday Avenue, Carlsbad, CA 92008, USA
    J Biol Chem 279:36317-26. 2004
  3. ncbi Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice
    Susana M Cerritelli
    Laboratory of Molecular Genetics, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 11:807-15. 2003
  4. ncbi Inhibition of HIV-1 ribonuclease H by a novel diketo acid, 4-[5-(benzoylamino)thien-2-yl]-2,4-dioxobutanoic acid
    Cathryn A Shaw-Reid
    Department of Biological Chemistry, Merck Research Laboratories, West Point, Pennsylvania 19486 0004, USA
    J Biol Chem 278:2777-80. 2003
  5. ncbi Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis
    Marcin Nowotny
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 121:1005-16. 2005
  6. ncbi Mutations at position 184 of human immunodeficiency virus type-1 reverse transcriptase affect virus titer and viral DNA synthesis
    John G Julias
    Basic Research Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702 1201, USA
    Virology 322:13-21. 2004
  7. ncbi Uncovering the complexities of retroviral ribonuclease H reveals its potential as a therapeutic target
    George J Klarmann
    Reverse Transcriptase Biochemistry Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, P O Box B Frederick, MD 21702, USA
    AIDS Rev 4:183-94. 2002
  8. ncbi Potent gene-specific inhibitory properties of mixed-backbone antisense oligonucleotides comprised of 2'-deoxy-2'-fluoro-D-arabinose and 2'-deoxyribose nucleotides
    Chun Nam Lok
    Lady Davis Institute for Medical Research, Department of Medicine, McGill University, Montreal, Quebec PQ, Canada H3A 2K6
    Biochemistry 41:3457-67. 2002
  9. ncbi Examining Ty3 polypurine tract structure and function by nucleoside analog interference
    Chandravanu Dash
    Resistance Mechanisms Laboratory, HIV Drug Resistance Program, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
    J Biol Chem 281:2773-83. 2006
  10. pmc Inhibition of human immunodeficiency virus type 1 reverse transcriptase, RNase H, and integrase activities by hydroxytropolones
    Joël Didierjean
    Unité Propre de Recherche 9002 du CNRS conventionnée à l Université Louis Pasteur, IBMC, 15 rue Rene Descartes, 67084 Strasbourg Cedex, France
    Antimicrob Agents Chemother 49:4884-94. 2005

Research Grants

Detail Information

Publications145 found, 100 shown here

  1. pmc Mutations of the RNase H C helix of the Moloney murine leukemia virus reverse transcriptase reveal defects in polypurine tract recognition
    David Lim
    Integrated Program in Cellular, Molecular and Biophysical Studies, Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Virol 76:8360-73. 2002
    ..The data also suggest that the C helix may play an important role in polypurine tract recognition and proper formation of the plus-strand DNA's 5' end...
  2. ncbi Structural requirements at the catalytic site of the heteroduplex substrate for human RNase H1 catalysis
    Walt F Lima
    Department of Molecular and Structural Biology, Isis Pharmaceuticals, 2292 Faraday Avenue, Carlsbad, CA 92008, USA
    J Biol Chem 279:36317-26. 2004
    ....
  3. ncbi Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice
    Susana M Cerritelli
    Laboratory of Molecular Genetics, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 11:807-15. 2003
    ..These findings also have important implications for therapy of mitochondrial dysfunctions and drug development for the structurally related RNase H of HIV...
  4. ncbi Inhibition of HIV-1 ribonuclease H by a novel diketo acid, 4-[5-(benzoylamino)thien-2-yl]-2,4-dioxobutanoic acid
    Cathryn A Shaw-Reid
    Department of Biological Chemistry, Merck Research Laboratories, West Point, Pennsylvania 19486 0004, USA
    J Biol Chem 278:2777-80. 2003
    ....
  5. ncbi Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis
    Marcin Nowotny
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 121:1005-16. 2005
    ..In transposases, they are symmetrically coordinated and exchange roles to alternately activate a water and a 3'-OH for successive strand cleavage and transfer by a ping-pong mechanism...
  6. ncbi Mutations at position 184 of human immunodeficiency virus type-1 reverse transcriptase affect virus titer and viral DNA synthesis
    John G Julias
    Basic Research Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702 1201, USA
    Virology 322:13-21. 2004
    ..The M184T mutation increased the proportion of 2-LTR circle junctions containing a tRNA insertion, suggesting that the mutation affected the RNase H activity of RT...
  7. ncbi Uncovering the complexities of retroviral ribonuclease H reveals its potential as a therapeutic target
    George J Klarmann
    Reverse Transcriptase Biochemistry Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, P O Box B Frederick, MD 21702, USA
    AIDS Rev 4:183-94. 2002
    ..Our current understanding of retroviral RNase H function and the status of small molecule inhibitors are the focus of this review...
  8. ncbi Potent gene-specific inhibitory properties of mixed-backbone antisense oligonucleotides comprised of 2'-deoxy-2'-fluoro-D-arabinose and 2'-deoxyribose nucleotides
    Chun Nam Lok
    Lady Davis Institute for Medical Research, Department of Medicine, McGill University, Montreal, Quebec PQ, Canada H3A 2K6
    Biochemistry 41:3457-67. 2002
    ....
  9. ncbi Examining Ty3 polypurine tract structure and function by nucleoside analog interference
    Chandravanu Dash
    Resistance Mechanisms Laboratory, HIV Drug Resistance Program, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
    J Biol Chem 281:2773-83. 2006
    ....
  10. pmc Inhibition of human immunodeficiency virus type 1 reverse transcriptase, RNase H, and integrase activities by hydroxytropolones
    Joël Didierjean
    Unité Propre de Recherche 9002 du CNRS conventionnée à l Université Louis Pasteur, IBMC, 15 rue Rene Descartes, 67084 Strasbourg Cedex, France
    Antimicrob Agents Chemother 49:4884-94. 2005
    ..However, all tested compounds exhibited cellular toxicity that presently limits their applications...
  11. pmc Mutations in yeast replication proteins that increase CAG/CTG expansions also increase repeat fragility
    Julie L Callahan
    Department of Biology, Program in Genetics, Tufts University, Medford, Massachusetts 02155, USA
    Mol Cell Biol 23:7849-60. 2003
    ..These results suggest that processes that generate strand breaks, such as faulty Okazaki fragment processing or DNA repair, are an important source of TNR expansions...
  12. pmc Selective inhibitory DNA aptamers of the human RNase H1
    Frédéric Pileur
    INSERM U386, IFR Pathologies Infectieuses, Universite Victor Segalen Bordeaux 2, 146, rue Leo Saignat, 33076 Bordeaux Cedex, France
    Nucleic Acids Res 31:5776-88. 2003
    ..Finally, the inhibitory aptamers were capable of completely abolishing the action of an antisense oligonucleotide in a rabbit reticulocyte lysate supplemented with human RNase H1, with IC50 ranging from 50 to 100 nM...
  13. ncbi The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides
    Anneloor L M A ten Asbroek
    Neurozintuigen Laboratory, Academic Medical Center, Amsterdam, The Netherlands
    Eur J Biochem 269:583-92. 2002
    ..RNase H2 is involved in the activity assayed in vitro. The presence of cell-type specific factors affecting the activity and localization of RNase H2 is strongly suggested...
  14. ncbi Enzymatically amplified surface plasmon resonance imaging method using RNase H and RNA microarrays for the ultrasensitive detection of nucleic acids
    Terry T Goodrich
    Department of Chemistry, University of California Irvine, Irvine, California 92697, USA
    Anal Chem 76:6173-8. 2004
    ..Finally, in a series of kinetics measurements, the initial rate of hydrolysis is shown to depend directly on the surface concentration of DNA-RNA heteroduplexes...
  15. ncbi Efficient reduction of target RNAs by small interfering RNA and RNase H-dependent antisense agents. A comparative analysis
    Timothy A Vickers
    GeneTrove Division and Antisense Core Research Department, Isis Pharmaceuticals, Inc, Carlsbad, California 92008, USA
    J Biol Chem 278:7108-18. 2003
    ..These results demonstrate that siRNA oligonucleotide- and RNase H-dependent antisense strategies are both valid strategies for evaluating function of genes in cell-based assays...
  16. pmc Sequence, distance, and accessibility are determinants of 5'-end-directed cleavages by retroviral RNases H
    Sharon J Schultz
    Department of Microbiology, School of Medicine, University of Washington, Seattle, WA 98195, USA
    J Biol Chem 281:1943-55. 2006
    ..Together these finding demonstrate that the selection of 5'-end-directed cleavage sites by retroviral RNases H results from a combination of nucleotide sequence, permissible distance, and accessibility to the RNA 5'-end...
  17. ncbi Dissecting the effects of DNA polymerase and ribonuclease H inhibitor combinations on HIV-1 reverse-transcriptase activities
    Cathryn A Shaw-Reid
    Department of Antiviral Research, Merck Research Laboratories, West Point, Pennsylvania 19486 0004, USA
    Biochemistry 44:1595-606. 2005
    ....
  18. pmc Using pyrrolo-deoxycytosine to probe RNA/DNA hybrids containing the human immunodeficiency virus type-1 3' polypurine tract
    Chandravanu Dash
    Resistance Mechanisms Laboratory, HIV Drug Resistance Program, National Cancer Institute Frederick, Frederick, MD 21702, USA
    Nucleic Acids Res 32:1539-47. 2004
    ..pdC substitution within the template strand of a DNA duplex does not appear to significantly affect RT-catalyzed DNA synthesis. Implications of these findings on the use of pdC to examine nucleic acid structure are discussed...
  19. ncbi Nonpolar thymine isosteres in the Ty3 polypurine tract DNA template modulate processing and provide a model for its recognition by Ty3 reverse transcriptase
    Daniela Lener
    Resistance Mechanisms Laboratory, HIV Drug Resistance Program, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702 1201, USA
    J Biol Chem 278:26526-32. 2003
    ....
  20. ncbi A fluorescence-based high-throughput screening assay for inhibitors of human immunodeficiency virus-1 reverse transcriptase-associated ribonuclease H activity
    Michael A Parniak
    Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Anal Biochem 322:33-9. 2003
    ..Several chemical libraries totaling more than 106,000 compounds were screened with this assay in approximately 1 month...
  21. ncbi An unusual sugar conformation in the structure of an RNA/DNA decamer of the polypurine tract may affect recognition by RNase H
    Mary L Kopka
    Molecular Biology Institute, University of California, Los Angeles, CA 90095 1570, USA
    J Mol Biol 334:653-65. 2003
    ..Disruption could cause the enzyme to pause at the 5'-end of the PPT, leaving it intact. Pyrimidine-purine (YR) steps are most deformable and the T-A step especially can undergo A-to-B transitions readily...
  22. pmc Two-metal ion mechanism of RNA cleavage by HIV RNase H and mechanism-based design of selective HIV RNase H inhibitors
    Klaus Klumpp
    Roche Discovery Welwyn, Welwyn Garden City, UK
    Nucleic Acids Res 31:6852-9. 2003
    ..Based on this difference in active site structure, a series of small molecule N-hydroxyimides was identified with significant enzyme inhibitory potency and selectivity for HIV RNase H...
  23. ncbi 'Binding, bending and bonding': polypurine tract-primed initiation of plus-strand DNA synthesis in human immunodeficiency virus
    Jason W Rausch
    Reverse Transcriptase Biochemistry Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, Building 535, Room 325, Frederick, MD 21702, USA
    Int J Biochem Cell Biol 36:1752-66. 2004
    ..In this review, plus-strand primer processing in HIV-1 is discussed from biochemical, structural, and historical perspectives. A comparative analysis of PPT-processing in different LTR-containing retroelements is also presented...
  24. ncbi Cotranscriptionally formed DNA:RNA hybrids mediate transcription elongation impairment and transcription-associated recombination
    Pablo Huertas
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avenida Reina, Mercedes 6, 41012 Seville, Spain
    Mol Cell 12:711-21. 2003
    ..These data support a model to explain the connection between recombination, transcription, and mRNA metabolism and provide a new perspective to understanding transcription-associated recombination...
  25. ncbi Two modes of HIV-1 polypurine tract cleavage are affected by introducing locked nucleic acid analogs into the (-) DNA template
    Chandravanu Dash
    Resistance Mechanisms Laboratory, HIV Drug Resistance Program, NCI Frederick, National Institutes of Health, Frederick, MD 21702, USA
    J Biol Chem 279:37095-102. 2004
    ....
  26. ncbi A capillary electrophoretic assay for ribonuclease H activity
    King C Chan
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, Center for Cancer Research, NCI at Frederick, Frederick, MD 21702, USA
    Anal Biochem 331:296-302. 2004
    ..The assay was broadly applicable to other RNases H, specifically those from human, Escherichia coli, and HIV-2, although product profiles varied for each enzyme...
  27. ncbi Crystal structure of Argonaute and its implications for RISC slicer activity
    Ji Joon Song
    Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 305:1434-7. 2004
    ..The architecture of the molecule and the placement of the PAZ and PIWI domains define a groove for substrate binding and suggest a mechanism for siRNA-guided mRNA cleavage...
  28. ncbi Determination of the role of the human RNase H1 in the pharmacology of DNA-like antisense drugs
    Hongjiang Wu
    Department of Core Technology, Isis Pharmaceuticals, Inc, Carlsbad, California 92008, USA
    J Biol Chem 279:17181-9. 2004
    ..Further, we show that there are several additional previously undescribed RNases H in human cells that may participate in the effects of DNA-like antisense oligonucleotides...
  29. ncbi Mutations in the ribonuclease H active site of HIV-RT reveal a role for this site in stabilizing enzyme-primer-template binding
    Jason V Cristofaro
    Department of Cell Biology and Molecular Genetics, University of Maryland College Park, Building 231, College Park, Maryland 20742, USA
    Biochemistry 41:10968-75. 2002
    ..In this regard RT is similar to other nucleic acid cleaving enzymes that show enhanced binding upon mutation of active site residues...
  30. ncbi Chimeric RNA-DNA molecular beacon assay for ribonuclease H activity
    J Rizzo
    Department of Chemistry, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA, 19104, USA
    Mol Cell Probes 16:277-83. 2002
    ..This method permits real-time detection of RNase H activity and a convenient approach to RNase H kinetic and mechanistic study...
  31. ncbi Efficient RNase H-directed cleavage of RNA promoted by antisense DNA or 2'F-ANA constructs containing acyclic nucleotide inserts
    Maria M Mangos
    Contribution from McGill University, Department of Chemistry, Montreal, QC, Canada
    J Am Chem Soc 125:654-61. 2003
    ....
  32. ncbi Altering the RNase H primer grip of human immunodeficiency virus reverse transcriptase modifies cleavage specificity
    Jason W Rausch
    HIV Drug Resistance Program, National Cancer Institute Frederick, Frederick, Maryland 21702, USA
    Biochemistry 41:4856-65. 2002
    ..Reduced polymerization-independent RNase H activity also significantly influenced the rate of DNA strand transfer, suggesting the donor template must be reduced in size below 13 nt before this event proceeds...
  33. ncbi Pre-existing distortions in nucleic acid structure aid polypurine tract selection by HIV-1 reverse transcriptase
    Mamuka Kvaratskhelia
    Reverse Transcriptase Biochemistry Section, Resistance Mechanisms Laboratory, HIV Drug Resistance Program, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
    J Biol Chem 277:16689-96. 2002
    ..Our data collectively suggest that the entire PPT sequence contributes to the structural distortion at the PPT/U3 junction, potentially providing a mechanism for its selective processing...
  34. pmc Stepwise analyses of metal ions in RNase H catalysis from substrate destabilization to product release
    Marcin Nowotny
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 25:1924-33. 2006
    ..Lastly, product release probably requires dissociation of metal ion A, which is inhibited by either high concentrations of divalent cations or mutation of an assisting protein residue...
  35. pmc Specific cleavages by RNase H facilitate initiation of plus-strand RNA synthesis by Moloney murine leukemia virus
    Sharon J Schultz
    Department of Microbiology, School of Medicine, University of Washington, Seattle 98195 7242, USA
    J Virol 77:5275-85. 2003
    ..These data suggest a model in which efficient initiation of plus-strand synthesis requires the generation of a gap immediately following the plus-strand primer 3' terminus...
  36. pmc Mechanism for nucleoside analog-mediated abrogation of HIV-1 replication: balance between RNase H activity and nucleotide excision
    Galina N Nikolenko
    Viral Mutation Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 102:2093-8. 2005
    ....
  37. ncbi Oligonucleotides comprised of alternating 2'-deoxy-2'-fluoro-beta-D-arabinonucleosides and D-2'-deoxyribonucleosides (2'F-ANA/DNA 'altimers') induce efficient RNA cleavage mediated by RNase H
    Kyung Lyum Min
    Department of Chemistry, McGill University, 801 Sherbrooke Street West, H3A 2K6, Montreal, QC, Canada
    Bioorg Med Chem Lett 12:2651-4. 2002
    ....
  38. pmc Effects of mutations in the G tract of the human immunodeficiency virus type 1 polypurine tract on virus replication and RNase H cleavage
    John G Julias
    HIV Drug Resistance Program, NCI Frederick, P O Box B, Building 539, Room 130A, Frederick, MD 21702 1201, USA
    J Virol 78:13315-24. 2004
    ..This result is consistent with the idea that the 3' end-processing reactions of retroviral integrases may help to generate defined ends from a heterogenous population of linear viral DNAs...
  39. pmc Mutations in the 5' end of the human immunodeficiency virus type 1 polypurine tract affect RNase H cleavage specificity and virus titer
    Mary Jane McWilliams
    HIV Drug Resistance Program, Frederick Cancer Research and Development Center, National Cancer Institute, Frederick, Maryland 21702 1201, USA
    J Virol 77:11150-7. 2003
    ..The mutants tested preferentially retained specific segments of the PPT, suggesting an effect on cleavage specificity. These results suggest that structural features of the PPT are important for its recognition and cleavage in vivo...
  40. ncbi Fabricating RNA microarrays with RNA-DNA surface ligation chemistry
    Hye Jin Lee
    Department of Chemistry, University of California Irvine, 92697, USA
    Anal Chem 77:7832-7. 2005
    ..These RNA microarrays can be used in the study of RNA-protein/RNA/aptamer bioaffinity interactions and for the enzymatically amplified SPRI detection of DNA in the presence of RNase H...
  41. ncbi Synthesis and properties of oligonucleotide chimeras containing 5'-amino-2'-deoxy-2'-fluoroarabinonucleosides
    Ekaterina Viazovkina
    Department of Chemistry, McGill University, Montreal, Quebec, Canada
    Nucleosides Nucleotides Nucleic Acids 22:1335-8. 2003
    ..The corresponding relative rates observed with the human enzyme were: gap DNA > 2'F-ANA(5'N) > 2'F-ANA > DNA(5'N)...
  42. ncbi Selective inhibition of HIV-1 reverse transcriptase (HIV-1 RT) RNase H by small RNA hairpins and dumbbells
    Rami N Hannoush
    Department of Chemistry, Otto Maass Chemistry Building, McGill University, 801 Sherbrooke Street West, Montreal, Quebec H3A 2K6, Canada
    Chembiochem 5:527-33. 2004
    ..The results described here suggest a noncompetitive binding mechanism, as outlined in the differential inhibitory characteristics of each of the nucleic acid aptamers against the bacterial, human, and viral RNase H homologues...
  43. ncbi Mutating conserved residues in the ribonuclease H domain of Ty3 reverse transcriptase affects specialized cleavage events
    Daniela Lener
    Reverse Transcriptase Biochemistry Section, Resistance Mechanisms Laboratory, HIV Drug Resistance Program, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
    J Biol Chem 277:26486-95. 2002
    ..DNA hybrid failed to support DNA strand transfer and release of the (+) strand polypurine tract primer from (+) RNA, suggesting that interrupting one or both of these events might account for the transposition defect...
  44. ncbi Expression of the C-terminus of HIV-1 reverse transcriptase p66 and p51 subunits as a single polypeptide with RNase H activity
    Roberto Zuniga
    Programa de Virologia, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, Independencia 1027, Santiago, Chile
    Protein Eng Des Sel 17:581-7. 2004
    ..The TCCR construct maintains Mn(2+)-dependent RNase H activity and specifically cleaves the substrate mimicking the tRNA removal required for second-strand transfer reactions...
  45. ncbi Human RNase H1 uses one tryptophan and two lysines to position the enzyme at the 3'-DNA/5'-RNA terminus of the heteroduplex substrate
    Walt F Lima
    Department of Molecular and Structural Biology, Isis Pharmaceuticals, Carlsbad, California 92008, USA
    J Biol Chem 278:49860-7. 2003
    ..These results offer further insights into the interaction between human RNase H1 and the heteroduplex substrate as well as approaches to enhance the design of effective antisense oligonucleotides...
  46. ncbi Interpreting oligonucleotide microarray data to determine RNA secondary structure: application to the 3' end of Bombyx mori R2 RNA
    Shenghua Duan
    Department of Chemistry, University of Rochester, Rochester, New York 14627 0216, USA
    Biochemistry 45:9819-32. 2006
    ..It should also facilitate the design of antisense and siRNA oligonucleotides...
  47. pmc Facilitating RNA structure prediction with microarrays
    Elzbieta Kierzek
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12 14, 61 704 Poznan, Poland
    Biochemistry 45:581-93. 2006
    ..The results suggest that probing RNA with oligonucleotide microarrays can facilitate determination of secondary structure...
  48. ncbi Creation of DNA overhangs by using modified DNA overhang cloning method
    Wei Li
    Laboratory of Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, People s Republic of China
    Appl Microbiol Biotechnol 75:703-9. 2007
    ..The creation of DNA overhangs by using complementary DNA oligonucleotides, T4 ligase, and RNase H is simpler and cheaper than that by using reverse transcriptase and RNase H...
  49. ncbi Effects of donor and acceptor RNA structures on the mechanism of strand transfer by HIV-1 reverse transcriptase
    Mark Nils Hanson
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Mol Biol 353:772-87. 2005
    ..Overall results support that the acceptor structure has a major influence on the efficiency and position of the invasion and terminus transfer steps...
  50. ncbi Biochemical characterization and functional complementation of ribonuclease HII and ribonuclease HIII from Chlamydophila pneumoniae AR39
    Rubing Liang
    College of Life Science and Technology, Shanghai Jiaotong University, 800 Dong Chuan Road, Shanghai 200240, China
    Microbiology 153:787-93. 2007
    ..coli RNase H, perhaps representing a more primordial type. This is believed to be the first in vivo functional study of Chlamydophila RNase Hs and the results should contribute to the analysis of RNase Hs of other parasite species...
  51. pmc Mutations in human immunodeficiency virus type 1 RNase H primer grip enhance 3'-azido-3'-deoxythymidine resistance
    Krista A Delviks-Frankenberry
    HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    J Virol 81:6837-45. 2007
    ....
  52. pmc Characterization of the polymerase and RNase H activities of human foamy virus reverse transcriptase
    Paul L Boyer
    HIV Drug Resistance Program, National Cancer Institute FCRDC, P O Box B, Building 539, Room 130A, Frederick, MD 21702 1201, USA
    J Virol 78:6112-21. 2004
    ..Although the sequence of the basic loop of FV RT is different from the basic loop of either Moloney leukemia virus RNase H or Escherichia coli RNase H, the FV RT basic loop appears to have a similar function...
  53. pmc Selection of mutations in the connection and RNase H domains of human immunodeficiency virus type 1 reverse transcriptase that increase resistance to 3'-azido-3'-dideoxythymidine
    Jessica H Brehm
    University of Pittsburgh School of Medicine, S818 Scaife Hall, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    J Virol 81:7852-9. 2007
    ....
  54. ncbi Acceptor RNA cleavage profile supports an invasion mechanism for HIV-1 minus strand transfer
    Yan Chen
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, New York 14642, USA
    J Biol Chem 280:14443-52. 2005
    ..Observations with this new approach lend additional support to the proposed multistep transfer mechanism...
  55. ncbi Single-stranded DNA aptamer RT1t49 inhibits RT polymerase and RNase H functions of HIV type 1, HIV type 2, and SIVCPZ RTs
    Jay D Kissel
    Department of Biology, Indiana University, Bloomington, Indiana 47405, USA
    AIDS Res Hum Retroviruses 23:699-708. 2007
    ..This is the first demonstration of universal inhibition of HIV and SIV(cpz) RTs by a nucleic acid aptamer and supports previous reports suggesting that resistance to RT1t49 may be exceptionally infrequent...
  56. pmc Specific recognition and cleavage of the plus-strand primer by reverse transcriptase
    Angela Atwood-Moore
    Section on Eukaryotic Transposable Elements, Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 79:14863-75. 2005
    ..Thus, our results identify a conserved element in RT that contacts the PPT and is specifically required for PPT removal...
  57. ncbi Evidence that HIV-1 reverse transcriptase employs the DNA 3' end-directed primary/secondary RNase H cleavage mechanism during synthesis and strand transfer
    Vandana Purohit
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 280:40534-43. 2005
    ..Overall, results indicate RT can catalyze pause-associated DNA 3' end-directed primary and secondary cuts during synthesis and these cuts can contribute to strand transfer by creation of an invasion site...
  58. ncbi DNA polymerization by the reverse transcriptase of the human L1 retrotransposon on its own template in vitro
    Olga Piskareva
    Institute of Biochemistry and Physiology of Microorganisms RAS Pushchino, Prosoekt Nauki 5, 142290 Pushchino, Moscow Region, Russia
    FEBS Lett 580:661-8. 2006
    ..A mechanism facilitating minus-strand DNA synthesis during reverse transcription of L1 element in vivo is discussed...
  59. pmc Forced selection of a human immunodeficiency virus type 1 variant that uses a non-self tRNA primer for reverse transcription: involvement of viral RNA sequences and the reverse transcriptase enzyme
    Truus E M Abbink
    Department of Human Retrovirology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    J Virol 78:10706-14. 2004
    ..We demonstrate that both the PAS and RT mutations improve the replication capacity of the tRNA(1,2)(Lys)-using virus...
  60. ncbi Synthesis, physicochemical and biochemical studies of 1',2'-oxetane constrained adenosine and guanosine modified oligonucleotides, and their comparison with those of the corresponding cytidine and thymidine analogues
    Pushpangadan I Pradeepkumar
    Contribution from the Department of Bioorganic Chemistry, Box 581, Biomedical Center, University of Uppsala, S 75123 Uppsala, Sweden
    J Am Chem Soc 126:11484-99. 2004
    ....
  61. ncbi Selection of optimal antisense accessible sites of survivin and its application in treatment of gastric cancer
    Qiang Song Tong
    Department of Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
    World J Gastroenterol 11:634-40. 2005
    ..To select the optimal antisense accessible sites of survivin, a highly expressed gene in tumor tissues, in order to explore a novel approach to improve biological therapy of gastric cancer...
  62. ncbi Reduced dNTP interaction of human immunodeficiency virus type 1 reverse transcriptase promotes strand transfer
    Darwin J Operario
    Departments of Microbiology and Immunology, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Biol Chem 281:32113-21. 2006
    ..Rather, kinetic interference leading to stalled DNA synthesis likely enhanced transfers. These results suggest that the RT-dNTP substrate interaction mechanistically influences strand transfer and recombination of HIV-1 RT...
  63. ncbi Highly selective action of triphosphate metabolite of 4'-ethynyl D4T: a novel anti-HIV compound against HIV-1 RT
    Guangwei Yang
    Department of Pharmacology, School of Medicine, Yale University, 333 Cedar Street, New Haven, CT 06520, USA
    Antiviral Res 73:185-91. 2007
    ..4'-Ed4TTP showed much less inhibitory effects toward major host DNA polymerases. Overall, our results suggest that 4'-Ed4TTP is the active form for anti-HIV-1 activity via its inhibitory effect against RT...
  64. ncbi The HIV-1 reverse transcriptase mutants G190S and G190A, which confer resistance to non-nucleoside reverse transcriptase inhibitors, demonstrate reductions in RNase H activity and DNA synthesis from tRNA(Lys, 3) that correlate with reductions in replicati
    J Wang
    Department of Medicine, University of Rochester School of Medicine and Dentistry, NY 14642, USA
    Virology 348:462-74. 2006
    ..It also suggests that high concentrations of efavirenz are unlikely to favor the selection of G190S clinically...
  65. ncbi Insights into the multiple roles of pausing in HIV-1 reverse transcriptase-promoted strand transfers
    Lu Gao
    Department of Biochemistry and Biophysics, University of Rochester, New York 14642, USA
    J Biol Chem 282:6222-31. 2007
    ..We interpret the new results to mean that pause sites can also serve to stall DNA synthesis, allowing acceptor invasions initiated earlier in the template to catch up with the primer terminus...
  66. ncbi Differential susceptibility of HIV-1 reverse transcriptase to inhibition by RNA aptamers in enzymatic reactions monitoring specific steps during genome replication
    Daniel M Held
    Department of Biology, Indiana University, Bloomington, Indiana 47405, USA
    J Biol Chem 281:25712-22. 2006
    ..Together, these results support a model wherein aptamers suppress viral replication by cumulative inhibition of RT at every stage of genome replication...
  67. ncbi Interaction of the Ty3 reverse transcriptase thumb subdomain with template-primer
    Arkadiusz Bibillo
    Reverse Transcriptase Biochemistry Section, Resistance Mechanisms Laboratory, HIV Drug Resistance Program, NCI, Frederick, National Institutes of Health, Frederick, Maryland 21702 1201, USA
    J Biol Chem 280:30282-90. 2005
    ..Mutations in the thumb subdomain of Ty3 also affected RNase H activity, suggesting a closer spatial relationship between its N- and C-terminal catalytic centers compared with HIV-1 RT...
  68. ncbi Combining mutations in HIV-1 reverse transcriptase with mutations in the HIV-1 polypurine tract affects RNase H cleavages involved in PPT utilization
    Mary Jane McWilliams
    HIV Drug Resistance Program, NCI Frederick, MD 21702 1201, USA
    Virology 348:378-88. 2006
    ..In contrast, the mutations in the PPT (alone) and RT (alone) affect the specificity of PPT cleavage but have much less effect on the overall rate of cleavage...
  69. ncbi Short partially double-stranded oligodeoxynucleotide induces reverse transcriptase/RNase H-mediated cleavage of HIV RNA and contributes to abrogation of infectivity of virions
    Alexey A Matskevich
    Institute of Medical Virology, University of Zurich, CH 8006 Zurich, Switzerland
    AIDS Res Hum Retroviruses 22:1220-30. 2006
    ..The pretreatment with ODN A strongly reduced the infectivity of virions in cell culture in the absence of any DNA carriers or detergents...
  70. ncbi Analysis of mutations made during active synthesis or extension of mismatched substrates further define the mechanism of HIV-RT mutagenesis
    Gwyneth R Liesch
    Department of Cell Biology and Molecular Genetics CBMG, University of Maryland College Park, College Park, Maryland 20742, USA
    Biochemistry 42:5925-36. 2003
    ..This is in contrast to RTs that must bind to then extend mismatches. The importance of these findings with respect to the mechanism of mutagenesis is discussed...
  71. ncbi Cooperative regulation for Okazaki fragment processing by RNase HII and FEN-1 purified from a hyperthermophilic archaeon, Pyrococcus furiosus
    Asako Sato
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan
    Biochem Biophys Res Commun 309:247-52. 2003
    ..It is likely that Pf-RNase HII and Pf-FEN-1 cooperatively process Okazaki fragment during lagging-strand DNA replication...
  72. ncbi Antisense oligonuclotides with oxetane-constrained cytidine enhance heteroduplex stability, and elicit satisfactory RNase H response as well as showing improved resistance to both exo and endonucleases
    Pushpangadan I Pradeepkumar
    Department of Bioorganic Chemistry, Biomedical Center, University of Uppsala, Box 581, S 751 23 Uppsala, Sweden
    Org Biomol Chem 1:81-92. 2003
    ....
  73. ncbi Comparative study of DNA enzymes and ribozymes against the same full-length messenger RNA of the vanilloid receptor subtype I
    Jens Kurreck
    Free University Berlin, Institute of Biochemistry, Thielallee 63, 14195 Berlin, Germany
    J Biol Chem 277:7099-107. 2002
    ..We therefore conclude that DNA enzymes are an inexpensive, very stable and active alternative to ribozymes for the specific cleavage of long RNA molecules...
  74. ncbi Efficient Hsp90-independent in vitro activation by Hsc70 and Hsp40 of duck hepatitis B virus reverse transcriptase, an assumed Hsp90 client protein
    Jürgen Beck
    Department of Internal Medicine II Molecular Biology, University Hospital Freiburg, Hugstetter Strasse 55, Freiburg D 79106, Germany
    J Biol Chem 278:36128-38. 2003
    ..Thus Hsc70, mostly known for its role in general protein folding, is able to effect activation of a highly specialized target protein...
  75. pmc Mutations in the RNase H domain of HIV-1 reverse transcriptase affect the initiation of DNA synthesis and the specificity of RNase H cleavage in vivo
    John G Julias
    HIV Drug Resistance Program, National Cancer Institute, National Institutes of Health, P O Box B, Frederick, MD 21702 1201, USA
    Proc Natl Acad Sci U S A 99:9515-20. 2002
    ..Analysis of two-long-terminal repeat circle junctions showed that mutations in the RNase H domain affect the specificity of RNase H cleavage...
  76. pmc Crosslinking of 4.5S RNA to the Escherichia coli ribosome in the presence or absence of the protein Ffh
    Jutta Rinke-Appel
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    RNA 8:612-25. 2002
    ..5S molecule, and this was docked onto the ribosome using the crosslinking data. The results are discussed in terms of multiple functions and binding sites of the 4.5S RNA...
  77. ncbi Strand transfer occurs in retroviruses by a pause-initiated two-step mechanism
    Ricardo H Roda
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 277:46900-11. 2002
    ..Nucleocapsid protein (NC) is known to influence primer elongation and strand exchange. The presence of NC increased the efficiency of transfers but did not appear to alter the fundamental transfer mechanism...
  78. ncbi Mechanism of minus strand strong stop transfer in HIV-1 reverse transcription
    Yan Chen
    Department of Biochemistry and Biophysics, University of Rochester, New York 14642, USA
    J Biol Chem 278:8006-17. 2003
    ..The acceptor invades at gaps created by reverse transcriptase-RNase H in the donor-cDNA hybrid. The fragmented donor is eventually strand-displaced by the acceptor, completing the transfer...
  79. ncbi Targeting degradation of RNA by RNase H using DNA hairpins
    Jing Li
    School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332 0230, USA
    Biochemistry 42:10945-54. 2003
    ..The in vitro results indicate that DNA hairpins can enhance the stringency of RNase H targeted degradation of the RNA sites...
  80. ncbi Cleavage of a DNA-RNA-DNA/DNA chimeric substrate containing a single ribonucleotide at the DNA-RNA junction with prokaryotic RNases HII
    Mitsuru Haruki
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2 1, Yamadaoka, Suita, Osaka, Japan
    FEBS Lett 531:204-8. 2002
    ..subtilis RNase HIII did not cleave the chimeric substrate containing a single ribonucleotide. These results suggest that bacterial and archaeal RNases HII are involved in excision of a single ribonucleotide misincorporated into DNA...
  81. ncbi Structural basis for the unusual properties of 2',5' nucleic acids and their complexes with RNA and DNA
    B J Premraj
    Department of Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India
    Biophys Chem 95:253-72. 2002
    ....
  82. ncbi RNA branching and debranching in the yeast retrovirus-like element Ty1
    Zhi Cheng
    School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110, USA
    Science 303:240-3. 2004
    ..The properties of the lariat suggest it forms by a novel mechanism and that branching and debranching may play roles in Ty1 reverse transcription at the minus-strand transfer step...
  83. pmc Evolutionary dynamics and population control during in vitro selection and amplification with multiple targets
    Hua Shi
    RNA 8:1461-70. 2002
    ..Additionally, it can be used to isolate a full spectrum of aptamers in a sequential and exhaustive manner for all the different targets in a mixture...
  84. pmc NMR structure of an alpha-L-LNA:RNA hybrid: structural implications for RNase H recognition
    Jakob T Nielsen
    Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, 5230 Odense M, Denmark
    Nucleic Acids Res 31:5858-67. 2003
    ..We attempt to rationalise this impaired enzyme activity from the rearrangement of the sugar-phosphate backbone of the alpha-L-LNA:RNA hybrid...
  85. pmc Dynamic NMR structures of [Rp]- and [Sp]-phosphorothioated DNA-RNA hybrids: is flexibility required for RNase H recognition?
    Marco Tonelli
    Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94143 2280 USA
    Biophys J 85:2525-38. 2003
    ..These observations, together with the analysis of the crystal structure of a hybrid duplex in complex with ribonuclease H (RNase H), suggested that this flexibility may be required for recognition by RNase H...
  86. pmc Inhibition of aminoglycoside 6'-N-acetyltransferase type Ib-mediated amikacin resistance by antisense oligodeoxynucleotides
    Renee Sarno
    Department of Biological Science, College of Natural Science and Mathematics, California State University Fullerton, Fullerton, California 92834 6850, USA
    Antimicrob Agents Chemother 47:3296-304. 2003
    ..Our results indicate that use of antisense compounds could be a viable strategy to preserve the efficacies of existing antibiotics to which bacteria are becoming increasingly resistant...
  87. ncbi Extension and cleavage of the polypurine tract plus-strand primer by Ty1 reverse transcriptase
    François Xavier Wilhelm
    Institut de Biologie Moleculaire et Cellulaire, 15, rue R Descartes, 67084 Strasbourg, France
    J Biol Chem 278:47678-84. 2003
    ....
  88. pmc The ribonuclease H activity of the reverse transcriptases of human immunodeficiency viruses type 1 and type 2 is modulated by residue 294 of the small subunit
    Z Sevilya
    Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Nucleic Acids Res 31:1481-7. 2003
    ..A comparative in vitro study of the kinetic parameters of the RNase H activity suggests that residue 294 affects the K(m) rather than the kcat value, influencing the affinity for the RNA.DNA substrate...
  89. ncbi Oligonucleotide enhanced cytotoxicity of Idarubicin for lymphoma cells
    J G Sharp
    J G Sharp Department of Cell Biology and Anatomy, University of Nebraska Medical Center, 986395 Nebraska Medical Center, Omaha, NE 68198 6395, USA
    Leuk Lymphoma 42:417-27. 2001
    ..This approach offers new opportunities for purging of lymphoma cells from hematopoietic harvests and systemic lymphoma therapy...
  90. ncbi Multiple mechanisms for initiation of ColE1 DNA replication: DNA synthesis in the presence and absence of ribonuclease H
    S DasGupta
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892
    Cell 51:1113-22. 1987
    ..Lagging strand synthesis terminates 17 nucleotides upstream of the normal replication origin, forcing unidirectional replication...
  91. ncbi Transcriptional activation of ColE1 DNA synthesis by displacement of the nontranscribed strand
    H Masukata
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892
    Cell 51:1123-30. 1987
    ..The necessary length for the single-stranded region is at least 40 nucleotides. The region probably provides a site for initial binding of a helicase that further unwinds the template DNA for initiation of DNA synthesis...
  92. ncbi Regulators of serine/threonine protein phosphatases at the dawn of a clinical era?
    R E Honkanen
    Department of Biochemistry and Molecular Biology, College of Medicine, University of South Alabama, Mobile, AL 36688, USA
    Curr Med Chem 9:2055-75. 2002
    ....
  93. ncbi Circular transcripts of the testis-determining gene Sry in adult mouse testis
    B Capel
    Laboratory of Eukaryotic Molecular Genetics, Medical Research Council, National Institute for Medical Research, London, England
    Cell 73:1019-30. 1993
    ..We suggest that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse...
  94. pmc Role of cysteine residues in ribonuclease H from Escherichia coli. Site-directed mutagenesis and chemical modification
    S Kanaya
    Protein Engineering Research Institute, Osaka, Japan
    Biochem J 271:59-66. 1990
    ..Thus the sensitivity of E. coli RNAase H to N-ethylmaleimide arises not from blocking of the thiol group but from steric hindrance by the modifying group incorporated at either Cys-13 or Cys-133...
  95. ncbi Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis
    J Minshull
    Department of Biochemistry, University of Cambridge, England
    Cell 56:947-56. 1989
    ..The extracts can enter mitosis if either of the cyclin mRNAs is left intact. We conclude that the synthesis of these cyclins is necessary for mitotic cell cycles in cleaving Xenopus embryos...
  96. pmc Crystal structure of the moloney murine leukemia virus RNase H domain
    David Lim
    Integrated Program in Cellular, Molecular and Biophysical Studies, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Virol 80:8379-89. 2006
    ..coli, and HIV-1 revealed that a loop of the HIV-1 connection domain resides within the same region of the Mo-MLV and E. coli C-helix. The HIV-1 connection domain may serve to recognize and bind the RNA/DNA substrate major groove...
  97. pmc Insertion of a small peptide of six amino acids into the beta7-beta8 loop of the p51 subunit of HIV-1 reverse transcriptase perturbs the heterodimer and affects its activities
    Pradeep K Pandey
    Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USA
    BMC Biochem 3:18. 2002
    ....
  98. pmc The identification of spermine binding sites in 16S rRNA allows interpretation of the spermine effect on ribosomal 30S subunit functions
    Ioannis Amarantos
    Laboratory of Biochemistry and Laboratory of Biology, School of Medicine, University of Patras, GR 26500 Patras, Greece
    Nucleic Acids Res 30:2832-43. 2002
    ..The above can be interpreted in terms of conformational changes in 16S rRNA, induced by the incorporation of ABA-spermine...
  99. pmc Role of integrase in reverse transcription of the Saccharomyces cerevisiae retrotransposon Ty1
    M Wilhelm
    Institut de Biologie Moleculaire et Cellulaire, 15 rue Rene Descartes, 67084 Strasbourg, France
    Eukaryot Cell 4:1057-65. 2005
    ..We suggest that interaction between these acidic amino acid residues of IN and a basic region of RT could be critical for the correct folding of RT and for the formation of an active conformation of the enzyme...
  100. pmc Mg2+ dependency of HIV-1 reverse transcription, inhibition by nucleoside analogues and resistance
    Valerie Goldschmidt
    Unité Propre de Recherche 9002 du CNRS conventionnée à l Université Louis Pasteur, IBMC, 15 rue Rene Descartes, 67084 Strasbourg Cedex, France
    Nucleic Acids Res 34:42-52. 2006
    ..Thus, differences in the free Mg2+ concentration between different cell types or during the cell cycle might strongly affect HIV-1 replication and its inhibition...
  101. ncbi Solution structure of DNA/RNA hybrid duplex with C8-propynyl 2'-deoxyadenosine modifications: Implication of RNase H and DNA/RNA duplex interaction
    Hunjoong Lee
    Department of Chemistry, University of Houston, 136 Fleming Building, Houston, TX 77204 5003, USA
    Biochim Biophys Acta 1769:20-8. 2007
    ....

Research Grants61

  1. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 2005
    ..coli CysRS for its ability to distinguish cysteine from serine. These studies shall shed new light on the molecular interactions responsible for the accurate translation of the genetic code. ..
  2. COMPREHENSIVE NATIONAL COOP DRUG DISCOVERY GROUP-AIDS
    Ellis Reinherz; Fiscal Year: 1993
    ..The potential for delivering these compounds to HIV-infected cells via the CD4 structure will be investigated...
  3. Stem Cell Quality Assays: Correlation with Aging/Health
    JOHN GRAHAM SHARP; Fiscal Year: 2010
    ..This research will validate laboratory assays of stem cell compartment quality which can then be applied to clinical situations and may reveal blood cytokine assays with surrogate predictive value. ..
  4. Maintenance of the tRNA 3' end
    Ya Ming Hou; Fiscal Year: 2008
    ..Because of the importance of CCA in maintenance of cellular life, the proposed studies will have a strong impact on human health and diseases. ..
  5. GnRH signaling in LbetaT2 gonadotrope cells
    Nicholas Webster; Fiscal Year: 2008
    ..These genetic models will include cell-specific transgenic overexpression of selected co-repressors as well as gonadotrope-specific deletion of components of the Gs and Gq/11 pathways using cre-loxP technology. ..
  6. Genetic conflict shapes centromeres and heterochromatin
    HARMIT MALIK; Fiscal Year: 2008
    ....
  7. Stem Cell Quality Assays: Correlation with Aging/Health
    John Sharp; Fiscal Year: 2008
    ..This research will validate laboratory assays of stem cell compartment quality which can then be applied to clinical situations and may reveal blood cytokine assays with surrogate predictive value. ..
  8. Maintenance of the tRNA 3' end
    Ya Ming Hou; Fiscal Year: 2007
    ..Christine Chow and Dr. Ming-Daw Tsai. PHS 398 (Rev. 5/95) (Form Page 4) Page 2 EE Number pages consecutivelyat the bottom throughout the application. Do not use suffixes such as 3a, 3b. ..
  9. Role of Tetraspan Glycoproteins (CD63) in HIV Replication
    MONIQUE FERGUSON; Fiscal Year: 2007
    ....
  10. Stem Cell Quality Assays: Correlation with Aging/Health
    John Sharp; Fiscal Year: 2007
    ..This research will validate laboratory assays of stem cell compartment quality which can then be applied to clinical situations and may reveal blood cytokine assays with surrogate predictive value. ..
  11. A Link between RNAi and Fragile X Mental Retardation
    Scott Hammond; Fiscal Year: 2007
    ..While this project focuses on FMRP regulation of neuronal genes, it will provide a general framework for studies on all aspects of RNAi regulation of mammalian cellular pathways. ..
  12. HIV RNase H natural product inhibitors
    MICHAEL PARNIAK; Fiscal Year: 2007
    ..The research program will have significant impact on public health by developing new anti-HIV therapeutics for use in the treatment of patients infected with HIV strains resistant to the current clinically used drugs. ..
  13. Genetic conflict shapes centromeres and heterochromatin
    HARMIT MALIK; Fiscal Year: 2007
    ....
  14. GnRH signaling in LbetaT2 gonadotrope cells
    Nicholas Webster; Fiscal Year: 2007
    ..These genetic models will include cell-specific transgenic overexpression of selected co-repressors as well as gonadotrope-specific deletion of components of the Gs and Gq/11 pathways using cre-loxP technology. ..
  15. A Link between RNAi and Fragile X Mental Retardation
    Scott Hammond; Fiscal Year: 2006
    ..While this project focuses on FMRP regulation of neuronal genes, it will provide a general framework for studies on all aspects of RNAi regulation of mammalian cellular pathways. ..
  16. A Link between RNAi and Fragile X Mental Retardation
    Scott Hammond; Fiscal Year: 2008
    ..While this project focuses on FMRP regulation of neuronal genes, it will provide a general framework for studies on all aspects of RNAi regulation of mammalian cellular pathways. ..
  17. Role of Tetraspan Glycoproteins (CD63) in HIV Replication
    MONIQUE FERGUSON; Fiscal Year: 2008
    ....
  18. Genetic conflict shapes centromeres and heterochromatin
    Harmit S Malik; Fiscal Year: 2010
    ....
  19. FUNCTIONS OF tRNA MODIFICATIONS
    Ya Ming Hou; Fiscal Year: 2009
    ..abstract_text> ..
  20. FUNCTIONS OF tRNA MODIFICATIONS
    Ya Ming Hou; Fiscal Year: 2010
    ..Information to be gained from this proposal will provide important insights to build a solid foundation for selective inhibition of the bacterial enzyme so as to improve human health care. ..
  21. Role of Tetraspan Glycoproteins (CD63) in HIV Replication
    MONIQUE REGAIL FERGUSON; Fiscal Year: 2010
    ....
  22. Role of Tetraspan Glycoproteins (CD63) in HIV Replication
    MONIQUE FERGUSON; Fiscal Year: 2009
    ....
  23. GnRH signaling in LbetaT2 gonadotrope cells
    Nicholas Webster; Fiscal Year: 2009
    ..These genetic models will include cell-specific transgenic overexpression of selected co-repressors as well as gonadotrope-specific deletion of components of the Gs and Gq/11 pathways using cre-loxP technology. ..
  24. FUNCTIONS OF tRNA MODIFICATIONS
    Ya Ming Hou; Fiscal Year: 2009
    ..Information to be gained from this proposal will provide important insights to build a solid foundation for selective inhibition of the bacterial enzyme so as to improve human health care. ..
  25. A Link between RNAi and Fragile X Mental Retardation
    Scott Hammond; Fiscal Year: 2009
    ..While this project focuses on FMRP regulation of neuronal genes, it will provide a general framework for studies on all aspects of RNAi regulation of mammalian cellular pathways. ..
  26. Role of Tetraspan Glycoproteins (CD63) in HIV Replication
    MONIQUE FERGUSON; Fiscal Year: 2009
    ....
  27. Genetic conflict shapes centromeres and heterochromatin
    HARMIT MALIK; Fiscal Year: 2009
    ....
  28. GnRH signaling in LbetaT2 gonadotrope cells
    Nicholas Webster; Fiscal Year: 2009
    ..These genetic models will include cell-specific transgenic overexpression of selected co-repressors as well as gonadotrope-specific deletion of components of the Gs and Gq/11 pathways using cre-loxP technology. ..
  29. FUNCTIONS OF tRNA MODIFICATIONS
    Ya Ming Hou; Fiscal Year: 2008
    ..Information to be gained from this proposal will provide important insights to build a solid foundation for selective inhibition of the bacterial enzyme so as to improve human health care. ..
  30. Stem Cell Quality Assays: Correlation with Aging/Health
    John Sharp; Fiscal Year: 2006
    ..This research will validate laboratory assays of stem cell compartment quality which can then be applied to clinical situations and may reveal blood cytokine assays with surrogate predictive value. ..
  31. GnRH signaling in LbetaT2 gonadotrope cells
    Nicholas Webster; Fiscal Year: 2006
    ..These genetic models will include cell-specific transgenic overexpression of selected co-repressors as well as gonadotrope-specific deletion of components of the Gs and Gq/11 pathways using cre-loxP technology. ..
  32. Genetic conflict shapes centromeres and heterochromatin
    HARMIT MALIK; Fiscal Year: 2006
    ....
  33. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 2004
    ..coli CysRS for its ability to distinguish cysteine from serine. These studies shall shed new light on the molecular interactions responsible for the accurate translation of the genetic code. ..
  34. Aminoacyl-tRNA synthetases in Archaea
    Ya Ming Hou; Fiscal Year: 2004
    ..jannaschii to gain a broader insight into its aminoacyl-tRNA synthetases as a basis for understanding the origin and evolution of contemporary mRNA-encoded protein synthesis in extant organisms. ..
  35. Aminoacyl-tRNA synthetases in Archaea
    Ya Ming Hou; Fiscal Year: 2003
    ..jannaschii to gain a broader insight into its aminoacyl-tRNA synthetases as a basis for understanding the origin and evolution of contemporary mRNA-encoded protein synthesis in extant organisms. ..
  36. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 2003
    ..coli CysRS for its ability to distinguish cysteine from serine. These studies shall shed new light on the molecular interactions responsible for the accurate translation of the genetic code. ..
  37. Mappicine inhibitors of HIV Ribonuclease H
    MICHAEL PARNIAK; Fiscal Year: 2003
    ..abstract_text> ..
  38. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 2002
    ..coli CysRS for its ability to distinguish cysteine from serine. These studies shall shed new light on the molecular interactions responsible for the accurate translation of the genetic code. ..
  39. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 2001
    ..The results of these studies will be integrated into structural studies of tRNACys and its complex with cysteine-tRNA synthetase in order to have a better understanding of tRNA structural recognition by an aminoacyl-tRNA synthetase. ..
  40. Stable suppression of gene function in mammals by RNAi
    Scott Hammond; Fiscal Year: 2002
    ..proposed commercial application: not available ..
  41. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 2001
    ..The results of these studies will be integrated into structural studies of tRNACys and its complex with cysteine-tRNA synthetase in order to have a better understanding of tRNA structural recognition by an aminoacyl-tRNA synthetase. ..
  42. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 2000
    ..The results of these studies will be integrated into structural studies of tRNACys and its complex with cysteine-tRNA synthetase in order to have a better understanding of tRNA structural recognition by an aminoacyl-tRNA synthetase. ..
  43. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 1999
    ..The results of these studies will be integrated into structural studies of tRNACys and its complex with cysteine-tRNA synthetase in order to have a better understanding of tRNA structural recognition by an aminoacyl-tRNA synthetase. ..
  44. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 1999
    ..The results of these studies will be integrated into structural studies of tRNACys and its complex with cysteine-tRNA synthetase in order to have a better understanding of tRNA structural recognition by an aminoacyl-tRNA synthetase. ..
  45. Maintenance of the tRNA 3' end
    Ya Ming Hou; Fiscal Year: 2004
    ..Because of the importance of CCA in maintenance of cellular life, the proposed studies will have a strong impact on human health and diseases. ..
  46. FUNCTIONAL ANALYSIS OF AN RNA STRUCTURAL MOTIF
    Ya Ming Hou; Fiscal Year: 2003
    ..coli CysRS for its ability to distinguish cysteine from serine. These studies shall shed new light on the molecular interactions responsible for the accurate translation of the genetic code. ..
  47. Aminoacyl-tRNA synthetases in Archaea
    Ya Ming Hou; Fiscal Year: 2006
    ..jannaschii to gain a broader insight into its aminoacyl-tRNA synthetases as a basis for understanding the origin and evolution of contemporary mRNA-encoded protein synthesis in extant organisms. [unreadable] [unreadable]..
  48. Maintenance of the tRNA 3' end
    Ya Ming Hou; Fiscal Year: 2007
    ..Because of the importance of CCA in maintenance of cellular life, the proposed studies will have a strong impact on human health and diseases. ..
  49. GnRH signaling in LbetaT2 gonadotrope cells
    Nicholas Webster; Fiscal Year: 2005
    ..These genetic models will include cell-specific transgenic overexpression of selected co-repressors as well as gonadotrope-specific deletion of components of the Gs and Gq/11 pathways using cre-loxP technology. ..
  50. A Link between RNAi and Fragile X Mental Retardation
    Scott Hammond; Fiscal Year: 2005
    ..While this project focuses on FMRP regulation of neuronal genes, it will provide a general framework for studies on all aspects of RNAi regulation of mammalian cellular pathways. ..
  51. A Bioinformatic Approach to Inferring Protein Contacts
    MARCELLA MCCLURE; Fiscal Year: 2005
    ..Several experimental laboratories have agreed to test the residue contact predictions resulting from these studies for Ebola, VSV and measles. ..
  52. Stem Cell Quality Assays: Correlation with Aging/Health
    John Sharp; Fiscal Year: 2005
    ..This research will validate laboratory assays of stem cell compartment quality which can then be applied to clinical situations and may reveal blood cytokine assays with surrogate predictive value. ..
  53. Rational Design of HIV Entry Inhibitors
    MONIQUE FERGUSON; Fiscal Year: 2005
    ..Since our peptides will be targeting viral structures, we expect them to have low toxicity, and no cross-resistance with the current antiretroviral drugs. ..
  54. Genetic conflict shapes centromeres and heterochromatin
    HARMIT MALIK; Fiscal Year: 2005
    ....
  55. Aminoacyl-tRNA synthetases in Archaea
    Ya Ming Hou; Fiscal Year: 2005
    ..jannaschii to gain a broader insight into its aminoacyl-tRNA synthetases as a basis for understanding the origin and evolution of contemporary mRNA-encoded protein synthesis in extant organisms. ..
  56. Maintenance of the tRNA 3' end
    Ya Ming Hou; Fiscal Year: 2005
    ..Because of the importance of CCA in maintenance of cellular life, the proposed studies will have a strong impact on human health and diseases. ..
  57. Combinatorial Approaches to Activators of the NGF Recep*
    Nicholas Webster; Fiscal Year: 2005
    ..The Specific Aim of this proposal is to screen the combinatorial asterriquinone library against the NGF receptor. ..