Genomes and Genes
Summary: A group of enzymes catalyzing the endonucleolytic cleavage of DNA. They include members of EC 3.1.21.-, EC 3.1.22.-, EC 3.1.23.- (DNA RESTRICTION ENZYMES), EC 3.1.24.- (DNA RESTRICTION ENZYMES), and EC 3.1.25.-.
Publications239 found, 100 shown here
- A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiationJing Pan
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
Cell 144:719-31. 2011..This map illuminates the occurrence of DSBs in repetitive DNA elements, repair of which can lead to chromosomal rearrangements. We also discuss implications for evolutionary dynamics of recombination hot spots...
- DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2Petr Cejka
Department of Microbiology, University of California, Davis, Davis, California 95616 8665, USA
Nature 467:112-6. 2010..We suggest that Top3-Rmi1 and MRX are important for recruitment of the Sgs1-Dna2 complex to DSBs. Our experiments provide a mechanistic framework for understanding the initial steps of recombinational DNA repair in eukaryotes...
- Highly efficient endogenous human gene correction using designed zinc-finger nucleasesFyodor D Urnov
Sangamo BioSciences, Inc, Pt Richmond Tech Center 501, Canal Blvd, Suite A100 Richmond, California 94804, USA
Nature 435:646-51. 2005..We observe comparably high frequencies in human T cells, raising the possibility of strategies based on zinc-finger nucleases for the treatment of disease...
- Crystal structure of the Mre11-Rad50-ATPγS complex: understanding the interplay between Mre11 and Rad50Hye Seong Lim
Department of Life Science, Pohang University of Science and Technology, Pohang, South Korea
Genes Dev 25:1091-104. 2011..Our structural and biochemical data provide insights into understanding the interplay between Mre11 and Rad50 to facilitate efficient DNA damage repair...
- ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repairGareth J Williams
Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
Nat Struct Mol Biol 18:423-31. 2011....
- Mre11-Rad50-Xrs2 and Sae2 promote 5' strand resection of DNA double-strand breaksMatthew L Nicolette
The Howard Hughes Medical Institute and the Department of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, Texas, USA
Nat Struct Mol Biol 17:1478-85. 2010..This stimulation is mainly caused by cooperative binding of DNA substrates by Exo1, MRX and Sae2. This work establishes the direct role of MRX and Sae2 in promoting the resection of 5' strands in DNA DSB repair...
- Endonuclease G regulates budding yeast life and deathSabrina Buttner
Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
Mol Cell 25:233-46. 2007..Deletion of NUC1 diminishes apoptotic death when mitochondrial respiration is increased but enhances necrotic death when oxidative phosphorylation is repressed, pointing to dual--lethal and vital--roles for EndoG...
- Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombinationMONICA P COLAIACOVO
Department of Developmental Biology, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
Dev Cell 5:463-74. 2003..Our data also suggest that early prophase barriers to utilization of sister chromatids as repair templates do not depend on central region assembly...
- Endonucleolytic processing of covalent protein-linked DNA double-strand breaksMatthew J Neale
Molecular Biology Programs, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Nature 436:1053-7. 2005..Our findings suggest a general mechanism for repair of protein-linked DSBs...
- Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaksEun Yong Shim
Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
EMBO J 29:3370-80. 2010..However, Mre11 nuclease activity is essential for resection in the absence of extensive resection enzymes. The results provide new insights into how MRX catalyses end resection and recombination initiation...
- The Mre11 complex is required for repair of hairpin-capped double-strand breaks and prevention of chromosome rearrangementsKirill S Lobachev
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
Cell 108:183-93. 2002..Our results suggest an additional role for the Mre11 complex in maintaining genome stability...
- The axial element protein HTP-3 promotes cohesin loading and meiotic axis assembly in C. elegans to implement the meiotic program of chromosome segregationAaron F Severson
Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
Genes Dev 23:1763-78. 2009..Premature equational sister separation in rec8 mutants of other organisms suggests the involvement of multiple REC-8 paralogs, which may have masked a conserved requirement for cohesin in AE assembly...
- The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repairKatja Lammens
Center for Integrated Protein Science Munich, Ludwig Maximilians University Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
Cell 145:54-66. 2011..The results suggest that MR is an ATP-controlled transient molecular clamp at DNA double-strand breaks...
- Identification and characterization of a family of mammalian methyl-CpG binding proteinsB Hendrich
Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland
Mol Cell Biol 18:6538-47. 1998..The data demonstrate that MBD2 and MBD4 bind specifically to methyl-CpG in vitro and in vivo and are therefore likely to be mediators of the biological consequences of the methylation signal...
- Caenorhabditis elegans HIM-18/SLX-4 interacts with SLX-1 and XPF-1 and maintains genomic integrity in the germline by processing recombination intermediatesTakamune T Saito
Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
PLoS Genet 5:e1000735. 2009..elegans BLM homolog. We propose that HIM-18 facilitates processing of HR intermediates resulting from replication fork collapse and programmed meiotic DSBs in the C. elegans germline...
- Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleasesInes Fonfara
Institut fur Biochemie, Justus Liebig Universitat Giessen, Heinrich Buff Ring 58, D 35392, Giessen, Germany
Nucleic Acids Res 40:847-60. 2012....
- The generation of proper constitutive G-tails on yeast telomeres is dependent on the MRX complexMichel Larrivée
Departement De Microbiologie et Infectiologie, Groupe ARN RNA Group, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, J1H 5N4 Canada
Genes Dev 18:1391-6. 2004..Thus, G-tails are present on yeast chromosomes throughout the cell cycle and the MRX complex is required for their normal establishment...
- Single-strand nicks induce homologous recombination with less toxicity than double-strand breaks using an AAV vector templateMichael J Metzger
Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Nucleic Acids Res 39:926-35. 2011....
- Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processingEleni P Mimitou
Department of Microbiology, Columbia University Medical Center, 701 West 168th Street, New York, New York 10032, USA
Nature 455:770-4. 2008..Second, Exo1 and/or Sgs1 rapidly process this intermediate to generate extensive tracts of single-stranded DNA that serve as substrate for Rad51...
- Systematic genetic analysis with ordered arrays of yeast deletion mutantsA H Tong
Banting and Best Department of Medical Research, University of Toronto, Toronto ON, Canada M5G 1L6
Science 294:2364-8. 2001..Systematic application of this approach should produce a global map of gene function...
- Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX complex or Sae2Eleni P Mimitou
Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY, USA
EMBO J 29:3358-69. 2010..Finally, we show that the presence of Ku exacerbates DNA end-processing defects established in the sae2Δ sgs1Δ mutant, leading to its lethality...
- A synaptonemal complex protein promotes homology-independent centromere couplingTomomi Tsubouchi
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
Science 308:870-3. 2005..Regions of synaptonemal complex assembled early in meiosis are often centromere-associated. We propose that centromere coupling facilitates homolog pairing and promotes synapsis initiation...
- Distinct properties of the XY pseudoautosomal region crucial for male meiosisLiisa Kauppi
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
Science 331:916-20. 2011..Our findings uncover specific mechanisms that surmount the unique challenges of X-Y recombination...
- DNA nicks promote efficient and safe targeted gene correctionLuther Davis
Department of Immunology, University of Washington School of Medicine, Seattle, Washington, United States of America
PLoS ONE 6:e23981. 2011..42±0.03%, respectively). Thus nicks initiate efficient gene correction, with limited local mutagenesis. These results have clear therapeutic implications, and should inform future design of meganucleases for targeted gene correction...
- DNA bending and a flip-out mechanism for base excision by the helix-hairpin-helix DNA glycosylase, Escherichia coli AlkAT Hollis
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
EMBO J 19:758-66. 2000..The structure of the AlkA-DNA complex offers the first glimpse of a helix-hairpin-helix (HhH) glycosylase complexed to DNA. Modeling studies suggest that other HhH glycosylases can bind to DNA in a similar manner...
- Mechanism and control of meiotic recombination initiationS Keeney
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, and Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
Curr Top Dev Biol 52:1-53. 2001....
- Homing endonucleases: from basics to therapeutic applicationsMaria J Marcaida
Macromolecular Crystallography Group, Structural Biology and Biocomputing Programme, Spanish National Cancer Research Centre CNIO, c Melchor Fdez Almagro 3, 28029 Madrid, Spain
Cell Mol Life Sci 67:727-48. 2010..The use of tailored HEs opens up new possibilities for gene therapy in patients with monogenic diseases that can be treated ex vivo. This review provides an overview of recent advances in this field...
- Progression of meiotic DNA replication is modulated by interchromosomal interaction proteins, negatively by Spo11p and positively by Rec8pR S Cha
Department of Molecular Biology, Harvard University, Cambridge, Massachusetts 02138 USA
Genes Dev 14:493-503. 2000..Roles for Rec8 and/or Spo11 in progression through other stages of meiosis are also revealed...
- The mouse Spo11 gene is required for meiotic chromosome synapsisP J Romanienko
Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health 20892, Bethesda, MD, USA
Mol Cell 6:975-87. 2000..We speculate that there is an additional role for Spo11, after it generates DSBs, in synapsis...
- Enhancing gene targeting with designed zinc finger nucleasesMarina Bibikova
Department of Biochemistry, University of Utah School of Medicine, 20 North 1900 East, Salt Lake City, UT 84132 3201, USA
Science 300:764. 2003
- Yeast nucleotide excision repair proteins Rad2 and Rad4 interact with RNA polymerase II basal transcription factor b (TFIIH)A J Bardwell
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235
Mol Cell Biol 14:3569-76. 1994..Furthermore, the loading of factor b (or such intermediates) onto promoters during transcription initiation provides a mechanism for the preferential targeting of repair proteins to actively transcribing genes...
- Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axesVérane Sommermeyer
Institut Curie, Centre de Recherche, 75248 Paris, France
Mol Cell 49:43-54. 2013..This paper provides the molecular mechanism linking DSB sequences to chromosome axes and explains why H3K4 methylation is important for meiotic recombination...
- A central coupler for recombination initiation linking chromosome architecture to S phase checkpointTomoichiro Miyoshi
Department of Life Sciences, The University of Tokyo, Komaba 3 8 1, Meguro ku, Tokyo 153 8902, Japan
Mol Cell 47:722-33. 2012..These data lead us to propose a mechanism by which Mde2 functions as a recombination initiation mediator to tether axes and loops, in liaison with the meiotic replication checkpoint...
- S. cerevisiae Tel1p and Mre11p are required for normal levels of Est1p and Est2p telomere associationLara K Goudsouzian
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
Mol Cell 24:603-10. 2006..These data argue that Mre11p and Tel1p affect telomere length by promoting telomerase recruitment to telomeres, whereas Mec1p has only a minor role in telomerase recruitment in a TEL1 cell...
- Mitochondrial release of AIF and EndoG requires caspase activation downstream of Bax/Bak-mediated permeabilizationDamien Arnoult
Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
EMBO J 22:4385-99. 2003..Thus EndoG and AIF seem to define a 'caspase-dependent' mitochondria-initiated apoptotic DNA degradation pathway that is conserved between mammals and nematodes...
- Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequencesJia Lin Ma
Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
Mol Cell Biol 23:8820-8. 2003..The increased gamma ray sensitivity of rad1Delta rad52Delta yku70Delta strains compared to rad52Delta yku70Delta strains suggests that MMEJ also contributes to the repair of DSBs induced by ionizing radiation...
- The Rad50 coiled-coil domain is indispensable for Mre11 complex functionsMarcel Hohl
Laboratory of Chromosome Biology, Memorial Sloan Kettering Cancer Center, New York, New York, USA
Nat Struct Mol Biol 18:1124-31. 2011..These data show that functions of Mre11 complex are integrated by the coiled coils of Rad50...
- Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82A A Mahdi
Department of Genetics, University of Nottingham Queens Medical Centre, UK
J Mol Biol 257:561-73. 1996..Multicopy plasmids expressing RusA alone make ruvA, ruvB, and ruvC mutants resistant to UV light. Suppression depends critically on RecG...
- Rec8 guides canonical Spo11 distribution along yeast meiotic chromosomesKazuto Kugou
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Tokyo 153 8902, Japan
Mol Biol Cell 20:3064-76. 2009..These observations suggest that Rec8 would prearrange the distribution of Spo11 along chromosomes and will provide clues to understanding temporal and spatial regulation of DSB formation...
- Functional interplay of the Mre11 nuclease and Ku in the response to replication-associated DNA damageSteven S Foster
Laboratory of Chromosome Biology, Memorial Sloan Kettering Cancer Center, 1275 York Ave, RRL 901C, New York, NY 10021, USA
Mol Cell Biol 31:4379-89. 2011..Collectively, the data define a nonhomologous end joining (NHEJ)-independent, S-phase-specific function of the Ku heterodimer...
- Divalent metal ion differentially regulates the sequential nicking reactions of the GIY-YIG homing endonuclease I-BmoIBenjamin P Kleinstiver
Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada
PLoS ONE 6:e23804. 2011..Collectively, our data implies mechanistic roles for divalent metal ion and substrate bases, suggesting that divalent metal ion facilitates the re-positioning of the GIY-YIG nuclease domain between sequential nicking reactions...
- Gamma radiation increases endonuclease-dependent L1 retrotransposition in a cultured cell assayEvan A Farkash
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Nucleic Acids Res 34:1196-204. 2006..The mobilization of L1 in irradiated cells potentially contributes to genomic instability and could be a driving force for secondary mutations in patients undergoing radiation therapy...
- Activity, specificity and structure of I-Bth0305I: a representative of a new homing endonuclease familyGregory K Taylor
Graduate Program in Molecular and Cellular Biology and the Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98019, USA
Nucleic Acids Res 39:9705-19. 2011..The domain architecture and DNA recognition profile displayed by I-Bth0305I, which is the prototype of a homing lineage that we term the 'EDxHD' family, are distinct from previously characterized homing endonucleases...
- Sae2 is an endonuclease that processes hairpin DNA cooperatively with the Mre11/Rad50/Xrs2 complexBettina M Lengsfeld
Department of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX 78712, USA
Mol Cell 28:638-51. 2007....
- Distinct roles of Mus81, Yen1, Slx1-Slx4, and Rad1 nucleases in the repair of replication-born double-strand breaks by sister chromatid exchangeSandra Muñoz-Galván
Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Universidad de Sevilla CSIC, Seville, Spain
Mol Cell Biol 32:1592-603. 2012..These unique effects, which have not been detected in other studies unless double mutant combinations were used, indicate the formation of distinct alternatives for the repair of replication-born DSBs that require specific SSEs...
- DNA interstrand crosslink repair during G1 involves nucleotide excision repair and DNA polymerase zetaSovan Sarkar
Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
EMBO J 25:1285-94. 2006..We show that this combination of NER and TLS is the only pathway of ICL repair available to the cell in G1 phase and is essential for viability in the presence of DNA crosslinks...
- The Mre11 protein interacts with both Rad50 and the HerA bipolar helicase and is recruited to DNA following gamma irradiation in the archaeon Sulfolobus acidocaldariusAchim Quaiser
Univ Paris Sud, Institut de Genetique et Microbiologie, CNRS UMR 8621, Batiment 409, F 91405 Orsay cedex, France
BMC Mol Biol 9:25. 2008..Since genetic tools for hyperthermophilic archaea are just emerging, we utilized immuno-detection approaches to get the first in vivo data on the role(s) of these proteins in the hyperthermophilic crenarchaeon Sulfolobus acidocaldarius...
- Genetic and biochemical characterization of MbeA, the relaxase involved in plasmid ColE1 conjugative mobilizationAthanasia Varsaki
Sector of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 451 10 Ioannina, Greece
Mol Microbiol 48:481-93. 2003..Thus, MbeA is a variant of the common relaxase theme with a HEN signature motif, which has to be added to the canonical three histidine motif of previously reported relaxases...
- ATP driven structural changes of the bacterial Mre11:Rad50 catalytic head complexCarolin Möckel
Gene Center and Department of Biochemistry, Ludwig Maximilians University Munich, Feodor Lynen Strasse 25, 81377, Munich, Germany
Nucleic Acids Res 40:914-27. 2012..Our results suggest a possible mechanism for ATP-dependent DNA tethering and DSB processing by MR...
- Genetic and structural characterization of endA. A membrane-bound nuclease required for transformation of Streptococcus pneumoniaeA Puyet
Department of Biology, Brookhaven National Laboratory, Upton, NY 11973
J Mol Biol 213:727-38. 1990..Neither of these upstream genes, nor the downstream gene, are essential for either cell viability or transformability...
- Important role of the nucleotide excision repair pathway in Mycobacterium smegmatis in conferring protection against commonly encountered DNA-damaging agentsKrishna Kurthkoti
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India
Microbiology 154:2776-85. 2008..Taken together with previous observations on NER-deficient M. tuberculosis, these results suggest that NER is an important DNA repair pathway in mycobacteria...
- Adaptation of intronic homing endonuclease for successful horizontal transmissionSayuri Kurokawa
Graduate School of Engineering, Department of Environmental Systems Engineering, Kochi University of Technology KUT, Kochi, Japan
FEBS J 272:2487-96. 2005....
- Atomic structure of the Serratia marcescens endonuclease at 1.1 A resolution and the enzyme reaction mechanismS V Shlyapnikov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str 32, Moscow 117984, Russia
Acta Crystallogr D Biol Crystallogr 56:567-72. 2000..marcescens nuclease structure, the functional characteristics of the natural and mutational forms of the enzyme and consideration of its structural analogy with homing endo-nuclease I-PpoI...
- Lethal anemia caused by interferon-beta produced in mouse embryos carrying undigested DNAHideyuki Yoshida
Department of Genetics, Osaka University Medical School, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
Nat Immunol 6:49-56. 2005....
- Ctp1 is a cell-cycle-regulated protein that functions with Mre11 complex to control double-strand break repair by homologous recombinationOliver Limbo
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Mol Cell 28:134-46. 2007..Transcription of ctp1(+) is periodic during the cell cycle, with the onset of its expression coinciding with the start of DNA replication. These data suggest that regulation of Ctp1 underlies cell-cycle control of HR...
- Nucleases of the metallo-beta-lactamase family and their role in DNA and RNA metabolismZbigniew Dominski
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Crit Rev Biochem Mol Biol 42:67-93. 2007..This article reviews the cellular roles of nucleases of the metallo-beta-lactamase family and the recent advances in studying these proteins...
- The hSNM1 protein is a DNA 5'-exonucleaseJames Hejna
Oregon Health and Science University, Department of Molecular and Medical Genetics, 3181 SW Sam Jackson Park Road, Mail Code L103, Portland, OR 97239 3098, USA
Nucleic Acids Res 35:6115-23. 2007..The exonuclease activity requires the conserved beta-lactamase domain; site-directed mutagenesis of a conserved aspartate inactivates the exonuclease...
- Synapsis-dependent and -independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegansAmy J MacQueen
Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305 5329, USA
Genes Dev 16:2428-42. 2002....
- BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structuresY Wang
Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
Genes Dev 14:927-39. 2000..Collectively, these results suggest that BRCA1 may function as a coordinator of multiple activities required for maintenance of genomic integrity during the process of DNA replication and point to a central role for BRCA1 in DNA repair...
- Stimulation of homology-directed gene targeting at an endogenous human locus by a nicking endonucleaseGijsbert P van Nierop
Virus and Stem Cell Biology Laboratory, Department of Molecular Cell Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands
Nucleic Acids Res 37:5725-36. 2009..Finally, harnessing the cellular HR pathway through Rep-mediated nicking expands the range of strategies that make use of AAV elements to bring about stable genetic modification of human cells...
- Investigation of the substrate spectrum of the human mismatch-specific DNA N-glycosylase MED1 (MBD4): fundamental role of the catalytic domainF Petronzelli
Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA
J Cell Physiol 185:473-80. 2000..This suggests that the catalytic domain is fundamental, and the 5-methylcytosine binding domain dispensable, in determining the substrate spectrum of MED1...
- Mre11 nuclease activity and Ctp1 regulate Chk1 activation by Rad3ATR and Tel1ATM checkpoint kinases at double-strand breaksOliver Limbo
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
Mol Cell Biol 31:573-83. 2011....
- Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleasesC Ban
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
EMBO J 17:1526-34. 1998..With sequence homology to Sau3AI and structural similarity to PvuII endonuclease, MutH is clearly related to these enzymes by divergent evolution, and this suggests that type II restriction endonucleases evolved from a common ancestor...
- 'Close-fitting sleeves': DNA damage recognition by the UvrABC nuclease systemBennett Van Houten
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, 111 Alexander Drive, MD D3 01, Research Triangle Park, NC 27709, USA
Mutat Res 577:92-117. 2005..This article reviews our current knowledge of how damage recognition is achieved in bacterial nucleotide excision repair through the concerted action of the UvrA, UvrB, and UvrC proteins...
- Recombinational DNA double-strand breaks in mice precede synapsisS K Mahadevaiah
Division of Developmental Genetics, National Institute for Medical Research, London, UK
Nat Genet 27:271-6. 2001..Loss of gamma-H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'...
- Single-step purifications of His6-MutH, His6-MutL and His6-MutS repair proteins of escherichia coli K-12G Feng
University of Texas, Houston Medical School, USA
Biotechniques 19:956-65. 1995..These constructs and purification methods should be useful to laboratories wishing to apply or develop MutS-mismatch mapping and to other applications or studies of the E. coli MutHLS repair proteins...
- Substrate specificity of the Escherichia coli Fpg protein (formamidopyrimidine-DNA glycosylase): excision of purine lesions in DNA produced by ionizing radiation or photosensitizationS Boiteux
URA 158 CNRS, U 140 INSERM, Institut Gustave Roussy, Villejuif, France
Biochemistry 31:106-10. 1992..The Fpg protein also excised FapyGua and 8-OH-Gua from visible light/MB-treated DNA. The presence of these products in the supernatant fractions confirmed their excision.(ABSTRACT TRUNCATED AT 250 WORDS)..
- MBD4 deficiency reduces the apoptotic response to DNA-damaging agents in the murine small intestineOwen James Sansom
Cardiff School of Biosciences, Cardiff University, PO Box 911, Cardiff CF10 3US, UK
Oncogene 22:7130-6. 2003..Our results establish a novel functional role for MBD4 in the cellular response to DNA damage and may have implications for its role in suppressing neoplasia...
- Leishmania infantum expresses a mitochondrial nuclease homologous to EndoG that migrates to the nucleus in response to an apoptotic stimulusEva Rico
Departamento de Bioquimica y Biologia Molecular, Campus Universitario, Universidad de Alcala, 28871 Alcala de Henares, Madrid, Spain
Mol Biochem Parasitol 163:28-38. 2009..Our results also demonstrate that overexpression of the nuclease in edelfosine-treated promastigotes causes a significant increase in the percentage of TUNEL-positive parasites...
- The small terminase, gp16, of bacteriophage T4 is a regulator of the DNA packaging motorAbdulrahman S Al-Zahrani
Department of Biology, The Catholic University of America, Washington, D C 20064, USA
J Biol Chem 284:24490-500. 2009..The gp16 regulator is essential to coordinate the gp17 motor ATPase, translocase, and nuclease activities, otherwise it could be suicidal to the virus...
- Crystal structure of the EndoG/EndoGI complex: mechanism of EndoG inhibitionBernhard Loll
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
Nucleic Acids Res 37:7312-20. 2009..The structure indicates that the two active sites of EndoG occupy the most remote spatial position possible at the molecular surface and a concerted substrate processing is unlikely...
- An intrastrand three-DNA-base interaction is a key specificity determinant of F transfer initiation and of F TraI relaxase DNA recognition and cleavageKatherine Hekman
Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
Nucleic Acids Res 36:4565-72. 2008..Oligonucleotide cleavage assay results suggest the essential function of the three-base interaction may be to position the scissile phosphate for cleavage, rather than to directly contribute to binding affinity...
- NucA is required for DNA cleavage during transformation of Bacillus subtilisR Provvedi
Public Health Research Institute, 455 First Avenue, New York 10016, USA
Mol Microbiol 40:634-44. 2001....
- Ctp1CtIP and Rad32Mre11 nuclease activity are required for Rec12Spo11 removal, but Rec12Spo11 removal is dispensable for other MRN-dependent meiotic functionsEdgar Hartsuiker
Genome Damage and Stability Centre, Sussex University, Brighton BN15 8HG, United Kingdom
Mol Cell Biol 29:1671-81. 2009..These observations suggest that the conserved role of the MRN complex in these meiotic functions is independent of Rec12(Spo11) removal...
- CRN-1, a Caenorhabditis elegans FEN-1 homologue, cooperates with CPS-6/EndoG to promote apoptotic DNA degradationJay Z Parrish
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
EMBO J 22:3451-60. 2003..Our results suggest that CRN-1/FEN-1 may play a critical role in switching the state of cells from DNA replication/repair to DNA degradation during apoptosis...
- The nuclease a-inhibitor complex is characterized by a novel metal ion bridgeMahua Ghosh
Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
J Biol Chem 282:5682-90. 2007....
- Reverse transcriptase and endonuclease activities encoded by Penelope-like retroelementsKonstantin I Pyatkov
Institute of Cell Biophysics, Pushchino 142290, Russia
Proc Natl Acad Sci U S A 101:14719-24. 2004..We propose that the Penelope EN cleaves the target DNA during transposition, generating a primer for reverse transcription. Our results show that an active Uri EN has been adopted by a retrotransposon...
- Proliferation failure and gamma radiation sensitivity of Fen1 null mutant mice at the blastocyst stageElisabeth Larsen
Centre for Molecular Biology and Neuroscience, Institute of Medical Microbiology, The National Hospital, University of Oslo, 0027 Oslo, Norway
Mol Cell Biol 23:5346-53. 2003..Our data thus provide in vivo evidence for an essential function of FEN1 in DNA repair, as well as in DNA replication...
- Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteinsHui Li
Department of Pathology, University of Washington, Box 357705, Seattle, WA 98195, USA
Nucleic Acids Res 37:1650-62. 2009....
- New insight into the recognition of branched DNA structure by junction-resolving enzymesAnne Cécile Déclais
Cancer Research UK Nucleic Acid Structure Research Group, MSI WTB complex, The University of Dundee, Dundee DD1 5EH, UK
Curr Opin Struct Biol 18:86-95. 2008..The comparison highlights the versatility of Holliday junction resolution, and extracts some general principles of recognition...
- Saccharomyces Ku70, mre11/rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damageS E Lee
Rosenstiel Center, Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
Cell 94:399-409. 1998..We suggest that escape from the DNA damage-induced G2/M checkpoint depends on the extent of ssDNA created at broken chromosome ends. RPA appears to play a key intermediate step in this adaptation...
- The DNA translocating ATPase of bacteriophage T4 packaging motorKiran R Kondabagil
Department of Biology, The Catholic University of America, Washington, DC 20064, USA
J Mol Biol 363:786-99. 2006..Since the catalytic motifs of the N-terminal ATPase are highly conserved among >200 large terminase sequences analyzed, these may represent common themes in phage and herpes viral DNA translocation...
- A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged regionA Sancar
Cell 33:249-60. 1983..We have also obtained evidence suggesting that the enzyme acts by the same mechanism on PydC photoproducts which are thought to be of primary importance in UV-induced mutagenesis...
- A unified genetic, computational and experimental framework identifies functionally relevant residues of the homing endonuclease I-BmoIBenjamin P Kleinstiver
Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON N6A 5C1, Canada
Nucleic Acids Res 38:2411-27. 2010....
- Switch from caspase-dependent to caspase-independent death during heart development: essential role of endonuclease G in ischemia-induced DNA processing of differentiated cardiomyocytesNúria Bahi
Laboratori d Investigacio, Hospital Arnau de Vilanova, Department of Ciències Mèdiques Bàsiques, Universitat de Lleida, Av Rovira Roure, 80 25198 Lleida, Spain
J Biol Chem 281:22943-52. 2006....
- Alpha-anomeric deoxynucleotides, anoxic products of ionizing radiation, are substrates for the endonuclease IV-type AP endonucleasesAlexander A Ishchenko
Groupe Réparation de l ADN, UMR 8126 CNRS, Institut Gustave Roussy, 94805 Villejuif Cedex, France
Biochemistry 43:15210-6. 2004..We propose that the novel substrate specificities of Nfo and Apn1 play an important role in counteracting oxidative DNA base damage...
- Mus81-Eme1 are essential components of a Holliday junction resolvaseM N Boddy
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Cell 107:537-48. 2001..The mus81 meiotic defect is rescued by expression of a bacterial Holliday junction resolvase. These findings constitute strong evidence that Mus81 and Eme1 are subunits of a nuclear Holliday junction resolvase...
- Interplay of Mre11 nuclease with Dna2 plus Sgs1 in Rad51-dependent recombinational repairMartin E Budd
Divisions of Biology and Chemistry, Caltech, Braun Laboratories, Pasadena, California, United States of America
PLoS ONE 4:e4267. 2009..We further show that sgs1Delta mre11-H125N, but not sgs1Delta, is very sensitive to IR, implicating the Sgs1 helicase in the Dna2-mediated pathway...
- Defining the bacteriophage T4 DNA packaging machine: evidence for a C-terminal DNA cleavage domain in the large terminase/packaging protein gp17Francisco J Rentas
Department of Biology, The Catholic University of America, 103 McCort Ward Hall, 620 Michigan Ave, N E Washington, DC 20064, USA
J Mol Biol 334:37-52. 2003..Although the DNA recognition mechanisms may be distinct, it appears that T4 and other phage terminases employ a common catalytic paradigm for phosphodiester bond cleavage that is used by numerous nucleases...
- The N-terminal ATPase site in the large terminase protein gp17 is critically required for DNA packaging in bacteriophage T4V B Rao
Department of Biology, The Catholic University of America, Washington, DC 20064, USA
J Mol Biol 314:401-11. 2001..The purified K166G mutant showed a loss of gp17-ATPase activity. The data, for the first time, implicated a specific ATPase center in the viral dsDNA packaging...
- Isolation and characterization of T4 bacteriophage gp17 terminase, a large subunit multimer with enhanced ATPase activityRichard G Baumann
Department of Biochemistry and Molecular Biology, University of Maryland Medical School, Baltimore, Maryland 21201 1503, USA
J Biol Chem 278:4618-27. 2003....
- A highly sensitive selection method for directed evolution of homing endonucleasesZhilei Chen
Center for Biophysics and Computational Biology, University of Illinois, Urbana, IL 61801, USA
Nucleic Acids Res 33:e154. 2005..003%. This system should also be readily applicable for directed evolution of other DNA cleavage enzymes...
- DNase1L2 suppresses biofilm formation by Pseudomonas aeruginosa and Staphylococcus aureusL Eckhart
Department of Dermatology, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
Br J Dermatol 156:1342-5. 2007..We have recently demonstrated that epidermal keratinocytes strongly express DNase1-like 2 (DNase1L2) in a differentiation-associated manner...
- Cleavage of insertion/deletion mismatches, flap and pseudo-Y DNA structures by deoxyinosine 3'-endonuclease from Escherichia coliM Yao
Division of Cancer Biology, Department of Radiation Oncology, Emory University School of Medicine, Atlanta, Georgia 30335, USA
J Biol Chem 271:30672-6. 1996..These biochemical properties suggest that deoxyinosine 3'-endonuclease might be important in the repair of IDM structures generated in lagging strand during DNA replication...
- The MRX complex stabilizes the replisome independently of the S phase checkpoint during replication stressMireille Tittel-Elmer
Department of Biochemistry and Molecular Biology, Southern Alberta Cancer Research Institute, University of Calgary, Calgary, Alberta, Canada
EMBO J 28:1142-56. 2009....
- Labeling of unique sequences in double-stranded DNA at sites of vicinal nicks generated by nicking endonucleasesHeiko Kuhn
Center for Advanced Biotechnology and Department of Biomedical Engineering, Boston University, MA 02215, USA
Nucleic Acids Res 36:e40. 2008..The method can find applications in sensitive and specific detection of viral duplex DNA...
- Prokaryotic nucleotide excision repair: the UvrABC systemJames J Truglio
Department of Pharmacological Sciences, State University of New York at Stony Brook, 11794 5115, USA
Chem Rev 106:233-52. 2006
- LEI/L-DNase II: interplay between caspase-dependent and independent pathwaysAlicia Torriglia
Universite Pierre et Marie Curie Paris 6, Paris, F 75006 France
Front Biosci (Landmark Ed) 14:4836-47. 2009..A caspase-activated DNase performs DNA degradation in caspase-dependent apoptosis, but other endonucleases like L-DNase II or GAAD are activated in caspase-independent apoptosis, allowing the complete dismantling of the cell...
- Crystallization and preliminary crystallographic analysis of a novel nuclease from Serratia marcescensM D Miller
Department of Biochemical and Biophysical Sciences, University of Houston, TX 77204 5934
J Mol Biol 222:27-30. 1991..7 A, b = 74.5 A, c = 68.9 A, and diffract to beyond 2 A. Low-resolution native data sets have been recorded and a search is under way for heavy-atom derivatives...
- An in vivo selection system for homing endonuclease activityMathias Gruen
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
Nucleic Acids Res 30:e29. 2002..This selection system may facilitate the study of sequence-specific DNA cleaving enzymes, and selections based on this work may enable the evolution of homing endonucleases with novel activities or specificities...
- Endonuclease V (nfi) mutant of Escherichia coli K-12G Guo
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109 0602, USA
J Bacteriol 180:46-51. 1998....
Research Grants158 found, 100 shown here
- RECOGNITION AND REPAIR OF UV DAMAGE TO HUMAN DNAYue Zou; Fiscal Year: 2002....
- PARTICIPATION OF PARP-1 IN THE PATHOGENESIS OF ASTHMAHamid Boulares; Fiscal Year: 2008..The results of the proposed study should provide insight into the suitability of PARP-1 as a novel target for the treatment of allergen-induced respiratory diseases such as asthma. ..
- Checkpoint Signaling and Repair of UV Damage to Human DNAYue Zou; Fiscal Year: 2008....
- Biochemical Insight of Hyperphosphorylation of Replication Protein AYue Zou; Fiscal Year: 2008..This project is highly relevant to cancer biology as DNA damage is the major cause to human cancer. [unreadable] [unreadable] [unreadable]..
- BIOLOGICALLY SIGNIFICANT DAMAGE IN DNAJAMIE MILLIGAN; Fiscal Year: 2007..Applications to human health include defining the causes of individual variation in radiosensitivity and the development of mechanistic models for risk estimation of cancer etiology by low dose and low dose rate exposures. ..
- PARTICIPATION OF PARP-1 IN THE PATHOGENESIS OF ASTHMAA Boulares; Fiscal Year: 2007..The results of the proposed study should provide insight into the suitability of PARP-1 as a novel target for the treatment of allergen-induced respiratory diseases such as asthma. ..
- PARTICIPATION OF PARP-1 IN THE PATHOGENESIS OF ASTHMAA Boulares; Fiscal Year: 2006..The results of the proposed study should provide insight into the suitability of PARP-1 as a novel target for the treatment of allergen-induced respiratory diseases such as asthma. ..
- BIOLOGICALLY SIGNIFICANT DAMAGE IN DNAJAMIE MILLIGAN; Fiscal Year: 2006..abstract_text> ..
- STRUCTURAL STUDIES OF INTRON ENCODED ENDONUCLEASESPatrick Van Roey; Fiscal Year: 2000..This will allow the comparison of representative examples of the two classes of enzymes that, although otherwise unrelated, carry out the same biological function. ..
- BIOLOGICALLY SIGNIFICANT DAMAGE IN DNAJAMIE MILLIGAN; Fiscal Year: 2005..abstract_text> ..
- RECOGNITION AND REPAIR OF UV DAMAGE TO HUMAN DNAYue Zou; Fiscal Year: 2001....
- PARTICIPATION OF PARP-1 IN THE PATHOGENESIS OF ASTHMAA Boulares; Fiscal Year: 2005
- STRUCTURAL STUDIES OF INTRON ENCODED ENDONUCLEASESPatrick Van Roey; Fiscal Year: 2001..This will allow the comparison of representative examples of the two classes of enzymes that, although otherwise unrelated, carry out the same biological function. ..
- PARTICIPATION OF PARP-1 IN THE PATHOGENESIS OF ASTHMAA Boulares; Fiscal Year: 2004
- RECOGNITION AND REPAIR OF UV DAMAGE TO HUMAN DNAYue Zou; Fiscal Year: 2004....
- RECOGNITION AND REPAIR OF UV DAMAGE TO HUMAN DNAYue Zou; Fiscal Year: 2003....
- BIOLOGICALLY SIGNIFICANT DAMAGE IN DNAJAMIE MILLIGAN; Fiscal Year: 2003..abstract_text> ..
- BIOLOGICALLY SIGNIFICANT DAMAGE IN DNAJAMIE MILLIGAN; Fiscal Year: 2004..abstract_text> ..
- Checkpoint Signaling and Repair of UV Damage to Human DNAYue Zou; Fiscal Year: 2009....
- PARTICIPATION OF PARP-1 IN THE PATHOGENESIS OF ASTHMAHamid Boulares; Fiscal Year: 2008
- BIOLOGICALLY SIGNIFICANT DAMAGE IN DNAJAMIE MILLIGAN; Fiscal Year: 2002..abstract_text> ..
- BIOLOGICALLY SIGNIFICANT DAMAGE IN DNAJAMIE MILLIGAN; Fiscal Year: 2010..Applications to human health include defining the causes of individual variation in radiosensitivity and the development of mechanistic models for risk estimation of cancer etiology by low dose and low dose rate exposures. ..
- BIOLOGICALLY SIGNIFICANT DAMAGE IN DNAJAMIE MILLIGAN; Fiscal Year: 2009..Applications to human health include defining the causes of individual variation in radiosensitivity and the development of mechanistic models for risk estimation of cancer etiology by low dose and low dose rate exposures. ..
- STRUCTURAL STUDIES OF INTRON ENCODED ENDONUCLEASESPatrick Van Roey; Fiscal Year: 1999..This will allow the comparison of representative examples of the two classes of enzymes that, although otherwise unrelated, carry out the same biological function. ..
- BIOLOGICALLY SIGNIFICANT DAMAGE IN DNAJAMIE MILLIGAN; Fiscal Year: 2008
- Mechanism of Nuclear Defects in Hutchinson-Gilford Progeria SyndromeYue Zou; Fiscal Year: 2008....
- Checkpoint Signaling and Repair of UV Damage to Human DNAYue Zou; Fiscal Year: 2010....
- Checkpoint Signaling and Repair of UV Damage to Human DNAYue Zou; Fiscal Year: 2007..abstract_text> ..
- A Multicomponent Anthrax Vaccine using Phage T4 DisplayVENIGALLA RAO; Fiscal Year: 2007..The duration of protection as well as pathological changes will be assessed. ..
- DNA REPAIR DEFECT IN FANCONI ANEMIA, GROUP AMURIEL LAMBERT; Fiscal Year: 2005..Thus elucidating the relationship between aSpllS* and the FANC proteins could potentially delineate the basis for defective hematopoietic differentiation and development, and for aplastic anemia, leukemia and other cancers in FA. ..
- Assembly Visualized Routine Flourescence MicroscopyPhilip Serwer; Fiscal Year: 2007..Applications are anticipated in the analysis of the assembly of disease-causing viruses and determining pathways of oncogenesis. [unreadable] [unreadable] [unreadable]..
- Function and Renal Regulation X/K -ATPase IsoformsBRUCE KONE; Fiscal Year: 2001..abstract_text> ..
- Mechanistic Studies of Nucleic Acid Damage and Their ApplicationMarc M Greenberg; Fiscal Year: 2010..Furthermore, the application of the knowledge gleaned from this research provides the starting point for potentially new therapeutics and research tools. ..
- Combined Methods for Protein-Structure PredictionKevin Karplus; Fiscal Year: 2003....
- Investigations of DNA Damage and RepairMarc Greenberg; Fiscal Year: 2005..Application of these studies, such as the design of enzyme inhibitors may provide new therapeutic agents. ..
- Transacting Genes Regulating Recombination Hotspot ActivitiesKenneth Paigen; Fiscal Year: 2009..Our project thus has relevance to all issues of health and disease that have any genetic component, and specifically to problems of tumorigenesis and infertility that involve DNA rearrangements and repair. ..
- FUNCTION AND REGULATION OF THE E COLI AIDB GENESMichael Volkert; Fiscal Year: 2001..During the proposed funding period, I propose to characterize this factor further by genetic and biochemical means and to compare its induction mechanism with that of meAda. ..
- Regulation of H/K-ATPase in Kidney and ColonBRUCE KONE; Fiscal Year: 2006..abstract_text> ..
- Genomic Organization of Recombination Hot SpotsKenneth Paigen; Fiscal Year: 2006..Additionally, for one of the chromosomal regions, we will carry out a genetic test for the existence of trans-acting genes required for hotspot activity. [unreadable] [unreadable]..
- Tetrahymena Genome Sequencing ProjectJonathan Eisen; Fiscal Year: 2004....
- GENETIC INSTABILITY IN WERNER SYNDROMERaymond Monnat; Fiscal Year: 2008..abstract_text> ..
- INTEGRATED STUDY OF MUSCULOSKELETAL LOSS AND RESTORATIONESTHER DUPONT VERSTEEGDEN; Fiscal Year: 2006..Identifying these common pathways influencing muscle and bone integrity could lead to putative targets for pharmacologic or therapeutic interventions. ..
- ENZYMATIC MECHANISMS OF GENETIC RECOMBINATIONRichard Kolodner; Fiscal Year: 2003..The ultimate goal of these studies will be to provide a comprehensive picture of the pathways and mechanisms that suppress genome instability. ..
- Mechanistic Studies of Nucleic Acid Damage and Their ApplicationMarc M Greenberg; Fiscal Year: 2010..Furthermore, the application of the knowledge gleaned from this research provides the starting point for potentially new therapeutics and research tools. ..
- Investigations of DNA Damage and RepairMarc Greenberg; Fiscal Year: 2002..Application of these studies, such as the design of enzyme inhibitors may provide new therapeutic agents. ..
- ANTITUMOR DRUGS AND INHIBITION OF INITIATION OF DNA REPLWILLIAM BURHANS; Fiscal Year: 2001..This project will explore these possibilities with a combined genetic and biochemical analysis of the effects of adozelesin and related drugs on initiation of DNA replication in mammalian cells. ..
- Mechanisms of Double-strand Break Repair in YeastLORRAINE SYMINGTON; Fiscal Year: 2008..The last aim includes in vitro studies to identify preferred DNA substrates for Rad55:Rad57 binding and stabilization of the Rad51 nucleoprotein by Rad55:Rad57 in the presence or absence of Srs2. ..
- HUMAN OXIDATION RESISTANCE GENESMichael Volkert; Fiscal Year: 2006..abstract_text> ..
- Improving Phenylbutyrate-based Anticancer TherapyASHOK BHAGWAT; Fiscal Year: 2005..This work will create the tools necessary for the monitoring of tumor cells in live animals when they are treated with PB as part of combination chemotherapy. ..
- Mechanisms of Mitotic RecombinationJeff Sekelsky; Fiscal Year: 2009..These studies will enhance our understanding of mechanisms of mitotic crossing over and the role that the important tumor suppressor protein BLM plays in preventing mitotic crossing over. ..
- Repair, Replication, and Detection of Oxidatively Damaged DNAMarc Greenberg; Fiscal Year: 2006..Furthermore, the reagents developed by us for selectively detecting oxidized abasic sites will be valuable biotechnology tools. ..
- Mutator Phenotype in Human CancersLAWRENCE LOEB; Fiscal Year: 2007..If increases in mutation frequency drive tumor progression, it is extremely important to identify agents that can inhibit mutagenesis and thereby prevent or impede the progression of human cancers. ..
- MECHANISM AND REGULATION OF NONHOMOLOGOUS DNA ENDJOININGMichael Lieber; Fiscal Year: 2006..The long-term medical benefit of a biochemically-defined NHEJ system includes the ability to test small molecule drug inhibitors for roles in cancer therapy. [unreadable] [unreadable]..
- ENZYMOLOGY OF MISMATCH REPAIR IN YEASTRichard D Kolodner; Fiscal Year: 2010..cerevisiae. As a consequence, it is anticipated that these studies will provide genetic and biochemical insights that can be applied to the study of the genetics of human cancer susceptibility. ..
- Transcription-induced MutationsASHOK BHAGWAT; Fiscal Year: 2007..Further, this system will be used to study the roles played by various DNA repair enzymes, DNA polymerases and accessory proteins in shaping the strand bias and spectrum of mutations responsible for antibody maturation. ..
- Mechanistic Studies of Nucleic Acid Damage and Their ApplicationMarc Greenberg; Fiscal Year: 2009..Furthermore, the application of the knowledge gleaned from this research provides the starting point for potentially new therapeutics and research tools. ..
- DRUG-INDUCED ATM READTHROUGH OF PTC MUTATIONSRichard A Gatti; Fiscal Year: 2010..These studies may impact upon other genetic disorders as well, and upon cancer patients with low levels of ATM protein. ..
- OXYGEN INDUCED DNA DAMAGE, MUTATIONS AND CANCERLAWRENCE LOEB; Fiscal Year: 2002..Even a modest, 2-fold, decrease could increase the latent period before disease is manifested clinically and thus reduce cancer mortality. ..
- ENZYMOLOGY OF MISMATCH REPAIR IN YEASTRichard Kolodner; Fiscal Year: 2001..In addition, it is anticipated that these studies will provide tools for use in the analysis of mismatch repair in higher eukaryotes. ..
- MECHANISM OF DNA RECOMBINATION AT CLASS SWITCH SEQUENCESMichael Lieber; Fiscal Year: 2001..Fifth, we search for enzymatic activities in extracts that carry out the cleavage step in class switch recombination. ..
- Impaired Regulation of Muscle size with AgingESTHER DUPONT VERSTEEGDEN; Fiscal Year: 2002..Also, potential regulatory pathways and molecules involved in control of muscle size will be determined. ..
- BIOPHYSICS AND GENETICS OF VIRAL DNA PACKAGINGPhilip Serwer; Fiscal Year: 1980..d) Development of dynamic models of the DNA packaging process from the above data and the testing of these models...
- TARGETING ORIGINS OF REPLICATION WITH CANCER DRUGSWILLIAM BURHANS; Fiscal Year: 2001..abstract_text> ..
- CORTICOLIMBIC ABNORMALITIES IN SCHIZOPHRENIC BRAINFrancine Benes; Fiscal Year: 2001....
- LETHAL MUTAGENESIS OF HIV BY DEOXYNUCLEOSIDE ANALOGSLAWRENCE LOEB; Fiscal Year: 2002....
- LDL isoforms in kidney-related atherosclerosisvAlexei Basnakian; Fiscal Year: 2008..If according to our expectation, cLDL is shown to be relevant to CKD-associated atherosclerosis, it may be used in the future as a reliable and mechanistically linked cardiovascular risk factor in CKD patients. ..
- SITE SPECIFIC RECOMBINATION IN THE YEAST PLASMIDMakkuni Jayaram; Fiscal Year: 1992..We would like to study the possible relevance of the recombination reaction to the physiology of 2 micron circle...
- ENZYMOLOGY OF MISMATCH REPAIR IN YEASTRichard Kolodner; Fiscal Year: 2008..cerevisiae. As a consequence, it is anticipated that these studies will provide genetic and biochemical insights that can be applied to the study of the genetics of human cancer susceptibility. ..
- ANTITUMOR DRUGS AND INHIBITION OF INITIATION OF DNA REPLWILLIAM BURHANS; Fiscal Year: 2000..This project will explore these possibilities with a combined genetic and biochemical analysis of the effects of adozelesin and related drugs on initiation of DNA replication in mammalian cells. ..
- Stable Maintenance of an Extrachromosomal Selfish DNA ElementMakkuni Jayaram; Fiscal Year: 2007..Some of the principles gleaned from this study will have implications in global symbiotic or commensalist relationships among host-parasite genomes. ..
- TARGETING ORIGINS OF REPLICATION WITH CANCER DRUGSWILLIAM BURHANS; Fiscal Year: 2000..abstract_text> ..
- VIRUS CHROMOSOME STRUCTURE--ROLE IN DEVELOPMENTMichael Feiss; Fiscal Year: 1999..These studies are directed at understanding how packaging protein interactions with DNA account for the major reconfiguring of packaging proteins that occurs during DNA packaging. ..
- Mechanisms of Error Prone Repair of DNA BreaksSang Eun Lee; Fiscal Year: 2007..unreadable] [unreadable]..
- Apoptosis in recombination-deficient meiocytesFrancesca Cole; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- CORTICOLIMBIC SYSTEM IN SCHIZOPHRENIC BRAIN--ANALYSISFrancine Benes; Fiscal Year: 1993....
- CORTICOLIMBIC SYSTEM IN SCHIZOPHRENIC BRAIN--ANALYSISFrancine Benes; Fiscal Year: 1991....
- INTEGRATED STUDY OF MUSCULOSKELETAL LOSS AND RESTORATIONESTHER DUPONT VERSTEEGDEN; Fiscal Year: 2008..Identifying these common pathways influencing muscle and bone integrity could lead to putative targets for pharmacologic or therapeutic interventions. ..
- CORTICOLIMBIC ABNORMALITIES IN SCHIZOPHRENIC BRAINFrancine Benes; Fiscal Year: 1999....
- Premortem enzymatic DNA damage in kidney injuryAlexei Basnakian; Fiscal Year: 2008..We propose that these two enzymes are linked in a pathway, in which DNase I activates endonuclease G, and that inactivation of any of these endonuclease can protect kidney from toxic injury. ..
- Stable Maintenance of an Extrachromosomal Selfish DNA ElementMakkuni Jayaram; Fiscal Year: 2010..Some of the principles gleaned from this study will have implications in global symbiotic or commensalist relationships among host-parasite genomes. ..
- Etiology of Chromosome TranslocationsSang Eun Lee; Fiscal Year: 2009..This application will identify and characterize damage- surveillance and other mechanisms that suppress chromosome translocation to understand the defects in blood cancers and develop novel preventive and/or therapeutic strategies. ..
- Gene Expression in Hippocampal Circuits of Bipolars and SchizophrenicsFrancine Benes; Fiscal Year: 2009....
- Mechanisms of Error Prone Repair of DNA BreaksSang Eun Lee; Fiscal Year: 2009..The information gleaned from the proposal will also provide a logical framework as to how cells tolerate cancer therapeutic agent-induced DNA lesions and shed lights on the prevention and improved therapeutic intervention of cancers. ..
- Premortem enzymatic DNA damage in kidney injuryAlexei Basnakian; Fiscal Year: 2009..We propose that these two enzymes are linked in a pathway, in which DNase I activates endonuclease G, and that inactivation of any of these endonuclease can protect kidney from toxic injury. ..