endodeoxyribonucleases

Summary

Summary: A group of enzymes catalyzing the endonucleolytic cleavage of DNA. They include members of EC 3.1.21.-, EC 3.1.22.-, EC 3.1.23.- (DNA RESTRICTION ENZYMES), EC 3.1.24.- (DNA RESTRICTION ENZYMES), and EC 3.1.25.-.

Top Publications

  1. pmc A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation
    Jing Pan
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Cell 144:719-31. 2011
  2. pmc DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2
    Petr Cejka
    Department of Microbiology, University of California, Davis, Davis, California 95616 8665, USA
    Nature 467:112-6. 2010
  3. ncbi Highly efficient endogenous human gene correction using designed zinc-finger nucleases
    Fyodor D Urnov
    Sangamo BioSciences, Inc, Pt Richmond Tech Center 501, Canal Blvd, Suite A100 Richmond, California 94804, USA
    Nature 435:646-51. 2005
  4. pmc Crystal structure of the Mre11-Rad50-ATPγS complex: understanding the interplay between Mre11 and Rad50
    Hye Seong Lim
    Department of Life Science, Pohang University of Science and Technology, Pohang, South Korea
    Genes Dev 25:1091-104. 2011
  5. pmc ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repair
    Gareth J Williams
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
    Nat Struct Mol Biol 18:423-31. 2011
  6. pmc Mre11-Rad50-Xrs2 and Sae2 promote 5' strand resection of DNA double-strand breaks
    Matthew L Nicolette
    The Howard Hughes Medical Institute and the Department of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, Texas, USA
    Nat Struct Mol Biol 17:1478-85. 2010
  7. ncbi Endonuclease G regulates budding yeast life and death
    Sabrina Buttner
    Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
    Mol Cell 25:233-46. 2007
  8. ncbi Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination
    MONICA P COLAIACOVO
    Department of Developmental Biology, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Dev Cell 5:463-74. 2003
  9. pmc Endonucleolytic processing of covalent protein-linked DNA double-strand breaks
    Matthew J Neale
    Molecular Biology Programs, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 436:1053-7. 2005
  10. pmc Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaks
    Eun Yong Shim
    Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
    EMBO J 29:3370-80. 2010

Detail Information

Publications239 found, 100 shown here

  1. pmc A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation
    Jing Pan
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Cell 144:719-31. 2011
    ..This map illuminates the occurrence of DSBs in repetitive DNA elements, repair of which can lead to chromosomal rearrangements. We also discuss implications for evolutionary dynamics of recombination hot spots...
  2. pmc DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2
    Petr Cejka
    Department of Microbiology, University of California, Davis, Davis, California 95616 8665, USA
    Nature 467:112-6. 2010
    ..We suggest that Top3-Rmi1 and MRX are important for recruitment of the Sgs1-Dna2 complex to DSBs. Our experiments provide a mechanistic framework for understanding the initial steps of recombinational DNA repair in eukaryotes...
  3. ncbi Highly efficient endogenous human gene correction using designed zinc-finger nucleases
    Fyodor D Urnov
    Sangamo BioSciences, Inc, Pt Richmond Tech Center 501, Canal Blvd, Suite A100 Richmond, California 94804, USA
    Nature 435:646-51. 2005
    ..We observe comparably high frequencies in human T cells, raising the possibility of strategies based on zinc-finger nucleases for the treatment of disease...
  4. pmc Crystal structure of the Mre11-Rad50-ATPγS complex: understanding the interplay between Mre11 and Rad50
    Hye Seong Lim
    Department of Life Science, Pohang University of Science and Technology, Pohang, South Korea
    Genes Dev 25:1091-104. 2011
    ..Our structural and biochemical data provide insights into understanding the interplay between Mre11 and Rad50 to facilitate efficient DNA damage repair...
  5. pmc ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repair
    Gareth J Williams
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
    Nat Struct Mol Biol 18:423-31. 2011
    ....
  6. pmc Mre11-Rad50-Xrs2 and Sae2 promote 5' strand resection of DNA double-strand breaks
    Matthew L Nicolette
    The Howard Hughes Medical Institute and the Department of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, Texas, USA
    Nat Struct Mol Biol 17:1478-85. 2010
    ..This stimulation is mainly caused by cooperative binding of DNA substrates by Exo1, MRX and Sae2. This work establishes the direct role of MRX and Sae2 in promoting the resection of 5' strands in DNA DSB repair...
  7. ncbi Endonuclease G regulates budding yeast life and death
    Sabrina Buttner
    Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
    Mol Cell 25:233-46. 2007
    ..Deletion of NUC1 diminishes apoptotic death when mitochondrial respiration is increased but enhances necrotic death when oxidative phosphorylation is repressed, pointing to dual--lethal and vital--roles for EndoG...
  8. ncbi Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination
    MONICA P COLAIACOVO
    Department of Developmental Biology, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Dev Cell 5:463-74. 2003
    ..Our data also suggest that early prophase barriers to utilization of sister chromatids as repair templates do not depend on central region assembly...
  9. pmc Endonucleolytic processing of covalent protein-linked DNA double-strand breaks
    Matthew J Neale
    Molecular Biology Programs, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 436:1053-7. 2005
    ..Our findings suggest a general mechanism for repair of protein-linked DSBs...
  10. pmc Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaks
    Eun Yong Shim
    Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
    EMBO J 29:3370-80. 2010
    ..However, Mre11 nuclease activity is essential for resection in the absence of extensive resection enzymes. The results provide new insights into how MRX catalyses end resection and recombination initiation...
  11. ncbi The Mre11 complex is required for repair of hairpin-capped double-strand breaks and prevention of chromosome rearrangements
    Kirill S Lobachev
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Cell 108:183-93. 2002
    ..Our results suggest an additional role for the Mre11 complex in maintaining genome stability...
  12. pmc The axial element protein HTP-3 promotes cohesin loading and meiotic axis assembly in C. elegans to implement the meiotic program of chromosome segregation
    Aaron F Severson
    Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    Genes Dev 23:1763-78. 2009
    ..Premature equational sister separation in rec8 mutants of other organisms suggests the involvement of multiple REC-8 paralogs, which may have masked a conserved requirement for cohesin in AE assembly...
  13. pmc The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repair
    Katja Lammens
    Center for Integrated Protein Science Munich, Ludwig Maximilians University Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Cell 145:54-66. 2011
    ..The results suggest that MR is an ATP-controlled transient molecular clamp at DNA double-strand breaks...
  14. pmc Identification and characterization of a family of mammalian methyl-CpG binding proteins
    B Hendrich
    Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland
    Mol Cell Biol 18:6538-47. 1998
    ..The data demonstrate that MBD2 and MBD4 bind specifically to methyl-CpG in vitro and in vivo and are therefore likely to be mediators of the biological consequences of the methylation signal...
  15. pmc Caenorhabditis elegans HIM-18/SLX-4 interacts with SLX-1 and XPF-1 and maintains genomic integrity in the germline by processing recombination intermediates
    Takamune T Saito
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Genet 5:e1000735. 2009
    ..elegans BLM homolog. We propose that HIM-18 facilitates processing of HR intermediates resulting from replication fork collapse and programmed meiotic DSBs in the C. elegans germline...
  16. pmc Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases
    Ines Fonfara
    Institut fur Biochemie, Justus Liebig Universitat Giessen, Heinrich Buff Ring 58, D 35392, Giessen, Germany
    Nucleic Acids Res 40:847-60. 2012
    ....
  17. pmc The generation of proper constitutive G-tails on yeast telomeres is dependent on the MRX complex
    Michel Larrivée
    Departement De Microbiologie et Infectiologie, Groupe ARN RNA Group, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, J1H 5N4 Canada
    Genes Dev 18:1391-6. 2004
    ..Thus, G-tails are present on yeast chromosomes throughout the cell cycle and the MRX complex is required for their normal establishment...
  18. pmc Single-strand nicks induce homologous recombination with less toxicity than double-strand breaks using an AAV vector template
    Michael J Metzger
    Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Nucleic Acids Res 39:926-35. 2011
    ....
  19. pmc Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processing
    Eleni P Mimitou
    Department of Microbiology, Columbia University Medical Center, 701 West 168th Street, New York, New York 10032, USA
    Nature 455:770-4. 2008
    ..Second, Exo1 and/or Sgs1 rapidly process this intermediate to generate extensive tracts of single-stranded DNA that serve as substrate for Rad51...
  20. ncbi Systematic genetic analysis with ordered arrays of yeast deletion mutants
    A H Tong
    Banting and Best Department of Medical Research, University of Toronto, Toronto ON, Canada M5G 1L6
    Science 294:2364-8. 2001
    ..Systematic application of this approach should produce a global map of gene function...
  21. pmc Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX complex or Sae2
    Eleni P Mimitou
    Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY, USA
    EMBO J 29:3358-69. 2010
    ..Finally, we show that the presence of Ku exacerbates DNA end-processing defects established in the sae2Δ sgs1Δ mutant, leading to its lethality...
  22. ncbi A synaptonemal complex protein promotes homology-independent centromere coupling
    Tomomi Tsubouchi
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Science 308:870-3. 2005
    ..Regions of synaptonemal complex assembled early in meiosis are often centromere-associated. We propose that centromere coupling facilitates homolog pairing and promotes synapsis initiation...
  23. pmc Distinct properties of the XY pseudoautosomal region crucial for male meiosis
    Liisa Kauppi
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Science 331:916-20. 2011
    ..Our findings uncover specific mechanisms that surmount the unique challenges of X-Y recombination...
  24. pmc DNA nicks promote efficient and safe targeted gene correction
    Luther Davis
    Department of Immunology, University of Washington School of Medicine, Seattle, Washington, United States of America
    PLoS ONE 6:e23981. 2011
    ..42±0.03%, respectively). Thus nicks initiate efficient gene correction, with limited local mutagenesis. These results have clear therapeutic implications, and should inform future design of meganucleases for targeted gene correction...
  25. pmc DNA bending and a flip-out mechanism for base excision by the helix-hairpin-helix DNA glycosylase, Escherichia coli AlkA
    T Hollis
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    EMBO J 19:758-66. 2000
    ..The structure of the AlkA-DNA complex offers the first glimpse of a helix-hairpin-helix (HhH) glycosylase complexed to DNA. Modeling studies suggest that other HhH glycosylases can bind to DNA in a similar manner...
  26. ncbi Mechanism and control of meiotic recombination initiation
    S Keeney
    Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, and Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Curr Top Dev Biol 52:1-53. 2001
    ....
  27. doi Homing endonucleases: from basics to therapeutic applications
    Maria J Marcaida
    Macromolecular Crystallography Group, Structural Biology and Biocomputing Programme, Spanish National Cancer Research Centre CNIO, c Melchor Fdez Almagro 3, 28029 Madrid, Spain
    Cell Mol Life Sci 67:727-48. 2010
    ..The use of tailored HEs opens up new possibilities for gene therapy in patients with monogenic diseases that can be treated ex vivo. This review provides an overview of recent advances in this field...
  28. pmc Progression of meiotic DNA replication is modulated by interchromosomal interaction proteins, negatively by Spo11p and positively by Rec8p
    R S Cha
    Department of Molecular Biology, Harvard University, Cambridge, Massachusetts 02138 USA
    Genes Dev 14:493-503. 2000
    ..Roles for Rec8 and/or Spo11 in progression through other stages of meiosis are also revealed...
  29. ncbi The mouse Spo11 gene is required for meiotic chromosome synapsis
    P J Romanienko
    Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health 20892, Bethesda, MD, USA
    Mol Cell 6:975-87. 2000
    ..We speculate that there is an additional role for Spo11, after it generates DSBs, in synapsis...
  30. ncbi Enhancing gene targeting with designed zinc finger nucleases
    Marina Bibikova
    Department of Biochemistry, University of Utah School of Medicine, 20 North 1900 East, Salt Lake City, UT 84132 3201, USA
    Science 300:764. 2003
  31. pmc Yeast nucleotide excision repair proteins Rad2 and Rad4 interact with RNA polymerase II basal transcription factor b (TFIIH)
    A J Bardwell
    Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235
    Mol Cell Biol 14:3569-76. 1994
    ..Furthermore, the loading of factor b (or such intermediates) onto promoters during transcription initiation provides a mechanism for the preferential targeting of repair proteins to actively transcribing genes...
  32. doi Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axes
    Vérane Sommermeyer
    Institut Curie, Centre de Recherche, 75248 Paris, France
    Mol Cell 49:43-54. 2013
    ..This paper provides the molecular mechanism linking DSB sequences to chromosome axes and explains why H3K4 methylation is important for meiotic recombination...
  33. doi A central coupler for recombination initiation linking chromosome architecture to S phase checkpoint
    Tomoichiro Miyoshi
    Department of Life Sciences, The University of Tokyo, Komaba 3 8 1, Meguro ku, Tokyo 153 8902, Japan
    Mol Cell 47:722-33. 2012
    ..These data lead us to propose a mechanism by which Mde2 functions as a recombination initiation mediator to tether axes and loops, in liaison with the meiotic replication checkpoint...
  34. ncbi S. cerevisiae Tel1p and Mre11p are required for normal levels of Est1p and Est2p telomere association
    Lara K Goudsouzian
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Mol Cell 24:603-10. 2006
    ..These data argue that Mre11p and Tel1p affect telomere length by promoting telomerase recruitment to telomeres, whereas Mec1p has only a minor role in telomerase recruitment in a TEL1 cell...
  35. pmc Mitochondrial release of AIF and EndoG requires caspase activation downstream of Bax/Bak-mediated permeabilization
    Damien Arnoult
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 22:4385-99. 2003
    ..Thus EndoG and AIF seem to define a 'caspase-dependent' mitochondria-initiated apoptotic DNA degradation pathway that is conserved between mammals and nematodes...
  36. pmc Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequences
    Jia Lin Ma
    Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
    Mol Cell Biol 23:8820-8. 2003
    ..The increased gamma ray sensitivity of rad1Delta rad52Delta yku70Delta strains compared to rad52Delta yku70Delta strains suggests that MMEJ also contributes to the repair of DSBs induced by ionizing radiation...
  37. pmc The Rad50 coiled-coil domain is indispensable for Mre11 complex functions
    Marcel Hohl
    Laboratory of Chromosome Biology, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Struct Mol Biol 18:1124-31. 2011
    ..These data show that functions of Mre11 complex are integrated by the coiled coils of Rad50...
  38. ncbi Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82
    A A Mahdi
    Department of Genetics, University of Nottingham Queens Medical Centre, UK
    J Mol Biol 257:561-73. 1996
    ..Multicopy plasmids expressing RusA alone make ruvA, ruvB, and ruvC mutants resistant to UV light. Suppression depends critically on RecG...
  39. pmc Rec8 guides canonical Spo11 distribution along yeast meiotic chromosomes
    Kazuto Kugou
    Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Tokyo 153 8902, Japan
    Mol Biol Cell 20:3064-76. 2009
    ..These observations suggest that Rec8 would prearrange the distribution of Spo11 along chromosomes and will provide clues to understanding temporal and spatial regulation of DSB formation...
  40. pmc Functional interplay of the Mre11 nuclease and Ku in the response to replication-associated DNA damage
    Steven S Foster
    Laboratory of Chromosome Biology, Memorial Sloan Kettering Cancer Center, 1275 York Ave, RRL 901C, New York, NY 10021, USA
    Mol Cell Biol 31:4379-89. 2011
    ..Collectively, the data define a nonhomologous end joining (NHEJ)-independent, S-phase-specific function of the Ku heterodimer...
  41. pmc Divalent metal ion differentially regulates the sequential nicking reactions of the GIY-YIG homing endonuclease I-BmoI
    Benjamin P Kleinstiver
    Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada
    PLoS ONE 6:e23804. 2011
    ..Collectively, our data implies mechanistic roles for divalent metal ion and substrate bases, suggesting that divalent metal ion facilitates the re-positioning of the GIY-YIG nuclease domain between sequential nicking reactions...
  42. pmc Gamma radiation increases endonuclease-dependent L1 retrotransposition in a cultured cell assay
    Evan A Farkash
    Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Nucleic Acids Res 34:1196-204. 2006
    ..The mobilization of L1 in irradiated cells potentially contributes to genomic instability and could be a driving force for secondary mutations in patients undergoing radiation therapy...
  43. pmc Activity, specificity and structure of I-Bth0305I: a representative of a new homing endonuclease family
    Gregory K Taylor
    Graduate Program in Molecular and Cellular Biology and the Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98019, USA
    Nucleic Acids Res 39:9705-19. 2011
    ..The domain architecture and DNA recognition profile displayed by I-Bth0305I, which is the prototype of a homing lineage that we term the 'EDxHD' family, are distinct from previously characterized homing endonucleases...
  44. pmc Sae2 is an endonuclease that processes hairpin DNA cooperatively with the Mre11/Rad50/Xrs2 complex
    Bettina M Lengsfeld
    Department of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX 78712, USA
    Mol Cell 28:638-51. 2007
    ....
  45. pmc Distinct roles of Mus81, Yen1, Slx1-Slx4, and Rad1 nucleases in the repair of replication-born double-strand breaks by sister chromatid exchange
    Sandra Muñoz-Galván
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Universidad de Sevilla CSIC, Seville, Spain
    Mol Cell Biol 32:1592-603. 2012
    ..These unique effects, which have not been detected in other studies unless double mutant combinations were used, indicate the formation of distinct alternatives for the repair of replication-born DSBs that require specific SSEs...
  46. pmc DNA interstrand crosslink repair during G1 involves nucleotide excision repair and DNA polymerase zeta
    Sovan Sarkar
    Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
    EMBO J 25:1285-94. 2006
    ..We show that this combination of NER and TLS is the only pathway of ICL repair available to the cell in G1 phase and is essential for viability in the presence of DNA crosslinks...
  47. pmc The Mre11 protein interacts with both Rad50 and the HerA bipolar helicase and is recruited to DNA following gamma irradiation in the archaeon Sulfolobus acidocaldarius
    Achim Quaiser
    Univ Paris Sud, Institut de Genetique et Microbiologie, CNRS UMR 8621, Batiment 409, F 91405 Orsay cedex, France
    BMC Mol Biol 9:25. 2008
    ..Since genetic tools for hyperthermophilic archaea are just emerging, we utilized immuno-detection approaches to get the first in vivo data on the role(s) of these proteins in the hyperthermophilic crenarchaeon Sulfolobus acidocaldarius...
  48. ncbi Genetic and biochemical characterization of MbeA, the relaxase involved in plasmid ColE1 conjugative mobilization
    Athanasia Varsaki
    Sector of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 451 10 Ioannina, Greece
    Mol Microbiol 48:481-93. 2003
    ..Thus, MbeA is a variant of the common relaxase theme with a HEN signature motif, which has to be added to the canonical three histidine motif of previously reported relaxases...
  49. pmc ATP driven structural changes of the bacterial Mre11:Rad50 catalytic head complex
    Carolin Möckel
    Gene Center and Department of Biochemistry, Ludwig Maximilians University Munich, Feodor Lynen Strasse 25, 81377, Munich, Germany
    Nucleic Acids Res 40:914-27. 2012
    ..Our results suggest a possible mechanism for ATP-dependent DNA tethering and DSB processing by MR...
  50. ncbi Genetic and structural characterization of endA. A membrane-bound nuclease required for transformation of Streptococcus pneumoniae
    A Puyet
    Department of Biology, Brookhaven National Laboratory, Upton, NY 11973
    J Mol Biol 213:727-38. 1990
    ..Neither of these upstream genes, nor the downstream gene, are essential for either cell viability or transformability...
  51. doi Important role of the nucleotide excision repair pathway in Mycobacterium smegmatis in conferring protection against commonly encountered DNA-damaging agents
    Krishna Kurthkoti
    Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India
    Microbiology 154:2776-85. 2008
    ..Taken together with previous observations on NER-deficient M. tuberculosis, these results suggest that NER is an important DNA repair pathway in mycobacteria...
  52. ncbi Adaptation of intronic homing endonuclease for successful horizontal transmission
    Sayuri Kurokawa
    Graduate School of Engineering, Department of Environmental Systems Engineering, Kochi University of Technology KUT, Kochi, Japan
    FEBS J 272:2487-96. 2005
    ....
  53. ncbi Atomic structure of the Serratia marcescens endonuclease at 1.1 A resolution and the enzyme reaction mechanism
    S V Shlyapnikov
    Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str 32, Moscow 117984, Russia
    Acta Crystallogr D Biol Crystallogr 56:567-72. 2000
    ..marcescens nuclease structure, the functional characteristics of the natural and mutational forms of the enzyme and consideration of its structural analogy with homing endo-nuclease I-PpoI...
  54. ncbi Lethal anemia caused by interferon-beta produced in mouse embryos carrying undigested DNA
    Hideyuki Yoshida
    Department of Genetics, Osaka University Medical School, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    Nat Immunol 6:49-56. 2005
    ....
  55. pmc Ctp1 is a cell-cycle-regulated protein that functions with Mre11 complex to control double-strand break repair by homologous recombination
    Oliver Limbo
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 28:134-46. 2007
    ..Transcription of ctp1(+) is periodic during the cell cycle, with the onset of its expression coinciding with the start of DNA replication. These data suggest that regulation of Ctp1 underlies cell-cycle control of HR...
  56. ncbi Nucleases of the metallo-beta-lactamase family and their role in DNA and RNA metabolism
    Zbigniew Dominski
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Crit Rev Biochem Mol Biol 42:67-93. 2007
    ..This article reviews the cellular roles of nucleases of the metallo-beta-lactamase family and the recent advances in studying these proteins...
  57. pmc The hSNM1 protein is a DNA 5'-exonuclease
    James Hejna
    Oregon Health and Science University, Department of Molecular and Medical Genetics, 3181 SW Sam Jackson Park Road, Mail Code L103, Portland, OR 97239 3098, USA
    Nucleic Acids Res 35:6115-23. 2007
    ..The exonuclease activity requires the conserved beta-lactamase domain; site-directed mutagenesis of a conserved aspartate inactivates the exonuclease...
  58. pmc Synapsis-dependent and -independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegans
    Amy J MacQueen
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305 5329, USA
    Genes Dev 16:2428-42. 2002
    ....
  59. pmc BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures
    Y Wang
    Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 14:927-39. 2000
    ..Collectively, these results suggest that BRCA1 may function as a coordinator of multiple activities required for maintenance of genomic integrity during the process of DNA replication and point to a central role for BRCA1 in DNA repair...
  60. pmc Stimulation of homology-directed gene targeting at an endogenous human locus by a nicking endonuclease
    Gijsbert P van Nierop
    Virus and Stem Cell Biology Laboratory, Department of Molecular Cell Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands
    Nucleic Acids Res 37:5725-36. 2009
    ..Finally, harnessing the cellular HR pathway through Rep-mediated nicking expands the range of strategies that make use of AAV elements to bring about stable genetic modification of human cells...
  61. ncbi Investigation of the substrate spectrum of the human mismatch-specific DNA N-glycosylase MED1 (MBD4): fundamental role of the catalytic domain
    F Petronzelli
    Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA
    J Cell Physiol 185:473-80. 2000
    ..This suggests that the catalytic domain is fundamental, and the 5-methylcytosine binding domain dispensable, in determining the substrate spectrum of MED1...
  62. pmc Mre11 nuclease activity and Ctp1 regulate Chk1 activation by Rad3ATR and Tel1ATM checkpoint kinases at double-strand breaks
    Oliver Limbo
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    Mol Cell Biol 31:573-83. 2011
    ....
  63. pmc Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases
    C Ban
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 17:1526-34. 1998
    ..With sequence homology to Sau3AI and structural similarity to PvuII endonuclease, MutH is clearly related to these enzymes by divergent evolution, and this suggests that type II restriction endonucleases evolved from a common ancestor...
  64. ncbi 'Close-fitting sleeves': DNA damage recognition by the UvrABC nuclease system
    Bennett Van Houten
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, 111 Alexander Drive, MD D3 01, Research Triangle Park, NC 27709, USA
    Mutat Res 577:92-117. 2005
    ..This article reviews our current knowledge of how damage recognition is achieved in bacterial nucleotide excision repair through the concerted action of the UvrA, UvrB, and UvrC proteins...
  65. ncbi Recombinational DNA double-strand breaks in mice precede synapsis
    S K Mahadevaiah
    Division of Developmental Genetics, National Institute for Medical Research, London, UK
    Nat Genet 27:271-6. 2001
    ..Loss of gamma-H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'...
  66. ncbi Single-step purifications of His6-MutH, His6-MutL and His6-MutS repair proteins of escherichia coli K-12
    G Feng
    University of Texas, Houston Medical School, USA
    Biotechniques 19:956-65. 1995
    ..These constructs and purification methods should be useful to laboratories wishing to apply or develop MutS-mismatch mapping and to other applications or studies of the E. coli MutHLS repair proteins...
  67. ncbi Substrate specificity of the Escherichia coli Fpg protein (formamidopyrimidine-DNA glycosylase): excision of purine lesions in DNA produced by ionizing radiation or photosensitization
    S Boiteux
    URA 158 CNRS, U 140 INSERM, Institut Gustave Roussy, Villejuif, France
    Biochemistry 31:106-10. 1992
    ..The Fpg protein also excised FapyGua and 8-OH-Gua from visible light/MB-treated DNA. The presence of these products in the supernatant fractions confirmed their excision.(ABSTRACT TRUNCATED AT 250 WORDS)..
  68. ncbi MBD4 deficiency reduces the apoptotic response to DNA-damaging agents in the murine small intestine
    Owen James Sansom
    Cardiff School of Biosciences, Cardiff University, PO Box 911, Cardiff CF10 3US, UK
    Oncogene 22:7130-6. 2003
    ..Our results establish a novel functional role for MBD4 in the cellular response to DNA damage and may have implications for its role in suppressing neoplasia...
  69. doi Leishmania infantum expresses a mitochondrial nuclease homologous to EndoG that migrates to the nucleus in response to an apoptotic stimulus
    Eva Rico
    Departamento de Bioquimica y Biologia Molecular, Campus Universitario, Universidad de Alcala, 28871 Alcala de Henares, Madrid, Spain
    Mol Biochem Parasitol 163:28-38. 2009
    ..Our results also demonstrate that overexpression of the nuclease in edelfosine-treated promastigotes causes a significant increase in the percentage of TUNEL-positive parasites...
  70. pmc The small terminase, gp16, of bacteriophage T4 is a regulator of the DNA packaging motor
    Abdulrahman S Al-Zahrani
    Department of Biology, The Catholic University of America, Washington, D C 20064, USA
    J Biol Chem 284:24490-500. 2009
    ..The gp16 regulator is essential to coordinate the gp17 motor ATPase, translocase, and nuclease activities, otherwise it could be suicidal to the virus...
  71. pmc Crystal structure of the EndoG/EndoGI complex: mechanism of EndoG inhibition
    Bernhard Loll
    Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
    Nucleic Acids Res 37:7312-20. 2009
    ..The structure indicates that the two active sites of EndoG occupy the most remote spatial position possible at the molecular surface and a concerted substrate processing is unlikely...
  72. pmc An intrastrand three-DNA-base interaction is a key specificity determinant of F transfer initiation and of F TraI relaxase DNA recognition and cleavage
    Katherine Hekman
    Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Nucleic Acids Res 36:4565-72. 2008
    ..Oligonucleotide cleavage assay results suggest the essential function of the three-base interaction may be to position the scissile phosphate for cleavage, rather than to directly contribute to binding affinity...
  73. ncbi NucA is required for DNA cleavage during transformation of Bacillus subtilis
    R Provvedi
    Public Health Research Institute, 455 First Avenue, New York 10016, USA
    Mol Microbiol 40:634-44. 2001
    ....
  74. pmc Ctp1CtIP and Rad32Mre11 nuclease activity are required for Rec12Spo11 removal, but Rec12Spo11 removal is dispensable for other MRN-dependent meiotic functions
    Edgar Hartsuiker
    Genome Damage and Stability Centre, Sussex University, Brighton BN15 8HG, United Kingdom
    Mol Cell Biol 29:1671-81. 2009
    ..These observations suggest that the conserved role of the MRN complex in these meiotic functions is independent of Rec12(Spo11) removal...
  75. pmc CRN-1, a Caenorhabditis elegans FEN-1 homologue, cooperates with CPS-6/EndoG to promote apoptotic DNA degradation
    Jay Z Parrish
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    EMBO J 22:3451-60. 2003
    ..Our results suggest that CRN-1/FEN-1 may play a critical role in switching the state of cells from DNA replication/repair to DNA degradation during apoptosis...
  76. pmc The nuclease a-inhibitor complex is characterized by a novel metal ion bridge
    Mahua Ghosh
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 282:5682-90. 2007
    ....
  77. pmc Reverse transcriptase and endonuclease activities encoded by Penelope-like retroelements
    Konstantin I Pyatkov
    Institute of Cell Biophysics, Pushchino 142290, Russia
    Proc Natl Acad Sci U S A 101:14719-24. 2004
    ..We propose that the Penelope EN cleaves the target DNA during transposition, generating a primer for reverse transcription. Our results show that an active Uri EN has been adopted by a retrotransposon...
  78. pmc Proliferation failure and gamma radiation sensitivity of Fen1 null mutant mice at the blastocyst stage
    Elisabeth Larsen
    Centre for Molecular Biology and Neuroscience, Institute of Medical Microbiology, The National Hospital, University of Oslo, 0027 Oslo, Norway
    Mol Cell Biol 23:5346-53. 2003
    ..Our data thus provide in vivo evidence for an essential function of FEN1 in DNA repair, as well as in DNA replication...
  79. pmc Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins
    Hui Li
    Department of Pathology, University of Washington, Box 357705, Seattle, WA 98195, USA
    Nucleic Acids Res 37:1650-62. 2009
    ....
  80. ncbi New insight into the recognition of branched DNA structure by junction-resolving enzymes
    Anne Cécile Déclais
    Cancer Research UK Nucleic Acid Structure Research Group, MSI WTB complex, The University of Dundee, Dundee DD1 5EH, UK
    Curr Opin Struct Biol 18:86-95. 2008
    ..The comparison highlights the versatility of Holliday junction resolution, and extracts some general principles of recognition...
  81. ncbi Saccharomyces Ku70, mre11/rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damage
    S E Lee
    Rosenstiel Center, Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Cell 94:399-409. 1998
    ..We suggest that escape from the DNA damage-induced G2/M checkpoint depends on the extent of ssDNA created at broken chromosome ends. RPA appears to play a key intermediate step in this adaptation...
  82. ncbi The DNA translocating ATPase of bacteriophage T4 packaging motor
    Kiran R Kondabagil
    Department of Biology, The Catholic University of America, Washington, DC 20064, USA
    J Mol Biol 363:786-99. 2006
    ..Since the catalytic motifs of the N-terminal ATPase are highly conserved among >200 large terminase sequences analyzed, these may represent common themes in phage and herpes viral DNA translocation...
  83. ncbi A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region
    A Sancar
    Cell 33:249-60. 1983
    ..We have also obtained evidence suggesting that the enzyme acts by the same mechanism on PydC photoproducts which are thought to be of primary importance in UV-induced mutagenesis...
  84. pmc A unified genetic, computational and experimental framework identifies functionally relevant residues of the homing endonuclease I-BmoI
    Benjamin P Kleinstiver
    Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON N6A 5C1, Canada
    Nucleic Acids Res 38:2411-27. 2010
    ....
  85. ncbi Switch from caspase-dependent to caspase-independent death during heart development: essential role of endonuclease G in ischemia-induced DNA processing of differentiated cardiomyocytes
    Núria Bahi
    Laboratori d Investigacio, Hospital Arnau de Vilanova, Department of Ciències Mèdiques Bàsiques, Universitat de Lleida, Av Rovira Roure, 80 25198 Lleida, Spain
    J Biol Chem 281:22943-52. 2006
    ....
  86. ncbi Alpha-anomeric deoxynucleotides, anoxic products of ionizing radiation, are substrates for the endonuclease IV-type AP endonucleases
    Alexander A Ishchenko
    Groupe Réparation de l ADN, UMR 8126 CNRS, Institut Gustave Roussy, 94805 Villejuif Cedex, France
    Biochemistry 43:15210-6. 2004
    ..We propose that the novel substrate specificities of Nfo and Apn1 play an important role in counteracting oxidative DNA base damage...
  87. ncbi Mus81-Eme1 are essential components of a Holliday junction resolvase
    M N Boddy
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 107:537-48. 2001
    ..The mus81 meiotic defect is rescued by expression of a bacterial Holliday junction resolvase. These findings constitute strong evidence that Mus81 and Eme1 are subunits of a nuclear Holliday junction resolvase...
  88. pmc Interplay of Mre11 nuclease with Dna2 plus Sgs1 in Rad51-dependent recombinational repair
    Martin E Budd
    Divisions of Biology and Chemistry, Caltech, Braun Laboratories, Pasadena, California, United States of America
    PLoS ONE 4:e4267. 2009
    ..We further show that sgs1Delta mre11-H125N, but not sgs1Delta, is very sensitive to IR, implicating the Sgs1 helicase in the Dna2-mediated pathway...
  89. ncbi Defining the bacteriophage T4 DNA packaging machine: evidence for a C-terminal DNA cleavage domain in the large terminase/packaging protein gp17
    Francisco J Rentas
    Department of Biology, The Catholic University of America, 103 McCort Ward Hall, 620 Michigan Ave, N E Washington, DC 20064, USA
    J Mol Biol 334:37-52. 2003
    ..Although the DNA recognition mechanisms may be distinct, it appears that T4 and other phage terminases employ a common catalytic paradigm for phosphodiester bond cleavage that is used by numerous nucleases...
  90. ncbi The N-terminal ATPase site in the large terminase protein gp17 is critically required for DNA packaging in bacteriophage T4
    V B Rao
    Department of Biology, The Catholic University of America, Washington, DC 20064, USA
    J Mol Biol 314:401-11. 2001
    ..The purified K166G mutant showed a loss of gp17-ATPase activity. The data, for the first time, implicated a specific ATPase center in the viral dsDNA packaging...
  91. ncbi Isolation and characterization of T4 bacteriophage gp17 terminase, a large subunit multimer with enhanced ATPase activity
    Richard G Baumann
    Department of Biochemistry and Molecular Biology, University of Maryland Medical School, Baltimore, Maryland 21201 1503, USA
    J Biol Chem 278:4618-27. 2003
    ....
  92. pmc A highly sensitive selection method for directed evolution of homing endonucleases
    Zhilei Chen
    Center for Biophysics and Computational Biology, University of Illinois, Urbana, IL 61801, USA
    Nucleic Acids Res 33:e154. 2005
    ..003%. This system should also be readily applicable for directed evolution of other DNA cleavage enzymes...
  93. ncbi DNase1L2 suppresses biofilm formation by Pseudomonas aeruginosa and Staphylococcus aureus
    L Eckhart
    Department of Dermatology, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Br J Dermatol 156:1342-5. 2007
    ..We have recently demonstrated that epidermal keratinocytes strongly express DNase1-like 2 (DNase1L2) in a differentiation-associated manner...
  94. ncbi Cleavage of insertion/deletion mismatches, flap and pseudo-Y DNA structures by deoxyinosine 3'-endonuclease from Escherichia coli
    M Yao
    Division of Cancer Biology, Department of Radiation Oncology, Emory University School of Medicine, Atlanta, Georgia 30335, USA
    J Biol Chem 271:30672-6. 1996
    ..These biochemical properties suggest that deoxyinosine 3'-endonuclease might be important in the repair of IDM structures generated in lagging strand during DNA replication...
  95. pmc The MRX complex stabilizes the replisome independently of the S phase checkpoint during replication stress
    Mireille Tittel-Elmer
    Department of Biochemistry and Molecular Biology, Southern Alberta Cancer Research Institute, University of Calgary, Calgary, Alberta, Canada
    EMBO J 28:1142-56. 2009
    ....
  96. pmc Labeling of unique sequences in double-stranded DNA at sites of vicinal nicks generated by nicking endonucleases
    Heiko Kuhn
    Center for Advanced Biotechnology and Department of Biomedical Engineering, Boston University, MA 02215, USA
    Nucleic Acids Res 36:e40. 2008
    ..The method can find applications in sensitive and specific detection of viral duplex DNA...
  97. ncbi Prokaryotic nucleotide excision repair: the UvrABC system
    James J Truglio
    Department of Pharmacological Sciences, State University of New York at Stony Brook, 11794 5115, USA
    Chem Rev 106:233-52. 2006
  98. ncbi LEI/L-DNase II: interplay between caspase-dependent and independent pathways
    Alicia Torriglia
    Universite Pierre et Marie Curie Paris 6, Paris, F 75006 France
    Front Biosci (Landmark Ed) 14:4836-47. 2009
    ..A caspase-activated DNase performs DNA degradation in caspase-dependent apoptosis, but other endonucleases like L-DNase II or GAAD are activated in caspase-independent apoptosis, allowing the complete dismantling of the cell...
  99. ncbi Crystallization and preliminary crystallographic analysis of a novel nuclease from Serratia marcescens
    M D Miller
    Department of Biochemical and Biophysical Sciences, University of Houston, TX 77204 5934
    J Mol Biol 222:27-30. 1991
    ..7 A, b = 74.5 A, c = 68.9 A, and diffract to beyond 2 A. Low-resolution native data sets have been recorded and a search is under way for heavy-atom derivatives...
  100. pmc An in vivo selection system for homing endonuclease activity
    Mathias Gruen
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Nucleic Acids Res 30:e29. 2002
    ..This selection system may facilitate the study of sequence-specific DNA cleaving enzymes, and selections based on this work may enable the evolution of homing endonucleases with novel activities or specificities...
  101. pmc Endonuclease V (nfi) mutant of Escherichia coli K-12
    G Guo
    Department of Pathology, University of Michigan Medical School, Ann Arbor 48109 0602, USA
    J Bacteriol 180:46-51. 1998
    ....

Research Grants158 found, 100 shown here

  1. BIOLOGICALLY SIGNIFICANT DAMAGE IN DNA
    JAMIE MILLIGAN; Fiscal Year: 2007
    ..Applications to human health include defining the causes of individual variation in radiosensitivity and the development of mechanistic models for risk estimation of cancer etiology by low dose and low dose rate exposures. ..
  2. PARTICIPATION OF PARP-1 IN THE PATHOGENESIS OF ASTHMA
    A Boulares; Fiscal Year: 2007
    ..The results of the proposed study should provide insight into the suitability of PARP-1 as a novel target for the treatment of allergen-induced respiratory diseases such as asthma. ..
  3. BIOLOGICALLY SIGNIFICANT DAMAGE IN DNA
    JAMIE MILLIGAN; Fiscal Year: 2006
    ..abstract_text> ..
  4. STRUCTURAL STUDIES OF INTRON ENCODED ENDONUCLEASES
    Patrick Van Roey; Fiscal Year: 2001
    ..This will allow the comparison of representative examples of the two classes of enzymes that, although otherwise unrelated, carry out the same biological function. ..
  5. RECOGNITION AND REPAIR OF UV DAMAGE TO HUMAN DNA
    Yue Zou; Fiscal Year: 2004
    ....
  6. Checkpoint Signaling and Repair of UV Damage to Human DNA
    Yue Zou; Fiscal Year: 2009
    ....
  7. BIOLOGICALLY SIGNIFICANT DAMAGE IN DNA
    JAMIE MILLIGAN; Fiscal Year: 2010
    ..Applications to human health include defining the causes of individual variation in radiosensitivity and the development of mechanistic models for risk estimation of cancer etiology by low dose and low dose rate exposures. ..
  8. Checkpoint Signaling and Repair of UV Damage to Human DNA
    Yue Zou; Fiscal Year: 2010
    ....
  9. Checkpoint Signaling and Repair of UV Damage to Human DNA
    Yue Zou; Fiscal Year: 2007
    ..abstract_text> ..
  10. A Multicomponent Anthrax Vaccine using Phage T4 Display
    VENIGALLA RAO; Fiscal Year: 2007
    ..The duration of protection as well as pathological changes will be assessed. ..
  11. DNA REPAIR DEFECT IN FANCONI ANEMIA, GROUP A
    MURIEL LAMBERT; Fiscal Year: 2005
    ..Thus elucidating the relationship between aSpllS* and the FANC proteins could potentially delineate the basis for defective hematopoietic differentiation and development, and for aplastic anemia, leukemia and other cancers in FA. ..
  12. Assembly Visualized Routine Flourescence Microscopy
    Philip Serwer; Fiscal Year: 2007
    ..Applications are anticipated in the analysis of the assembly of disease-causing viruses and determining pathways of oncogenesis. ..
  13. Mechanistic Studies of Nucleic Acid Damage and Their Application
    Marc M Greenberg; Fiscal Year: 2010
    ..Furthermore, the application of the knowledge gleaned from this research provides the starting point for potentially new therapeutics and research tools. ..
  14. Investigations of DNA Damage and Repair
    Marc Greenberg; Fiscal Year: 2005
    ..Application of these studies, such as the design of enzyme inhibitors may provide new therapeutic agents. ..
  15. Transacting Genes Regulating Recombination Hotspot Activities
    Kenneth Paigen; Fiscal Year: 2009
    ..Our project thus has relevance to all issues of health and disease that have any genetic component, and specifically to problems of tumorigenesis and infertility that involve DNA rearrangements and repair. ..
  16. Improving Phenylbutyrate-based Anticancer Therapy
    ASHOK BHAGWAT; Fiscal Year: 2005
    ..This work will create the tools necessary for the monitoring of tumor cells in live animals when they are treated with PB as part of combination chemotherapy. ..
  17. Mutator Phenotype in Human Cancers
    LAWRENCE LOEB; Fiscal Year: 2007
    ..If increases in mutation frequency drive tumor progression, it is extremely important to identify agents that can inhibit mutagenesis and thereby prevent or impede the progression of human cancers. ..
  18. ENZYMOLOGY OF MISMATCH REPAIR IN YEAST
    Richard D Kolodner; Fiscal Year: 2010
    ..cerevisiae. As a consequence, it is anticipated that these studies will provide genetic and biochemical insights that can be applied to the study of the genetics of human cancer susceptibility. ..
  19. Transcription-induced Mutations
    ASHOK BHAGWAT; Fiscal Year: 2007
    ..Further, this system will be used to study the roles played by various DNA repair enzymes, DNA polymerases and accessory proteins in shaping the strand bias and spectrum of mutations responsible for antibody maturation. ..
  20. Mechanistic Studies of Nucleic Acid Damage and Their Application
    Marc Greenberg; Fiscal Year: 2009
    ..Furthermore, the application of the knowledge gleaned from this research provides the starting point for potentially new therapeutics and research tools. ..
  21. DRUG-INDUCED ATM READTHROUGH OF PTC MUTATIONS
    Richard A Gatti; Fiscal Year: 2010
    ..These studies may impact upon other genetic disorders as well, and upon cancer patients with low levels of ATM protein. ..
  22. MECHANISM OF DNA RECOMBINATION AT CLASS SWITCH SEQUENCES
    Michael Lieber; Fiscal Year: 2001
    ..Fifth, we search for enzymatic activities in extracts that carry out the cleavage step in class switch recombination. ..
  23. Impaired Regulation of Muscle size with Aging
    ESTHER DUPONT VERSTEEGDEN; Fiscal Year: 2002
    ..Also, potential regulatory pathways and molecules involved in control of muscle size will be determined. ..
  24. BIOPHYSICS AND GENETICS OF VIRAL DNA PACKAGING
    Philip Serwer; Fiscal Year: 1980
    ..d) Development of dynamic models of the DNA packaging process from the above data and the testing of these models...
  25. CORTICOLIMBIC ABNORMALITIES IN SCHIZOPHRENIC BRAIN
    Francine Benes; Fiscal Year: 2001
    ....
  26. LETHAL MUTAGENESIS OF HIV BY DEOXYNUCLEOSIDE ANALOGS
    LAWRENCE LOEB; Fiscal Year: 2002
    ....
  27. Stable Maintenance of an Extrachromosomal Selfish DNA Element
    Makkuni Jayaram; Fiscal Year: 2007
    ..Some of the principles gleaned from this study will have implications in global symbiotic or commensalist relationships among host-parasite genomes. ..
  28. Mechanisms of Error Prone Repair of DNA Breaks
    Sang Eun Lee; Fiscal Year: 2007
    ..Since the components of DSB repair are conserved from yeast to humans, the insights garnered from our research will be valuable for dissecting the equivalent process in human cells and will be of relevance to public health. ..
  29. Apoptosis in recombination-deficient meiocytes
    Francesca Cole; Fiscal Year: 2007
    ....
  30. CORTICOLIMBIC SYSTEM IN SCHIZOPHRENIC BRAIN--ANALYSIS
    Francine Benes; Fiscal Year: 1993
    ....
  31. Stable Maintenance of an Extrachromosomal Selfish DNA Element
    Makkuni Jayaram; Fiscal Year: 2010
    ..Some of the principles gleaned from this study will have implications in global symbiotic or commensalist relationships among host-parasite genomes. ..
  32. Etiology of Chromosome Translocations
    Sang Eun Lee; Fiscal Year: 2009
    ..This application will identify and characterize damage- surveillance and other mechanisms that suppress chromosome translocation to understand the defects in blood cancers and develop novel preventive and/or therapeutic strategies. ..
  33. Gene Expression in Hippocampal Circuits of Bipolars and Schizophrenics
    Francine Benes; Fiscal Year: 2009
    ....
  34. Mechanisms of Error Prone Repair of DNA Breaks
    Sang Eun Lee; Fiscal Year: 2009
    ..The information gleaned from the proposal will also provide a logical framework as to how cells tolerate cancer therapeutic agent-induced DNA lesions and shed lights on the prevention and improved therapeutic intervention of cancers. ..