Genomes and Genes
Summary: A group of enzymes catalyzing the endonucleolytic cleavage of DNA. They include members of EC 3.1.21.-, EC 3.1.22.-, EC 3.1.23.- (DNA RESTRICTION ENZYMES), EC 3.1.24.- (DNA RESTRICTION ENZYMES), and EC 3.1.25.-.
Publications273 found, 100 shown here
- Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selectionVikram Pattanayak
Department of Chemistry and Chemical Biology, and Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA
Nat Methods 8:765-70. 2011..Our findings establish an energy compensation model of ZFN specificity in which excess binding energy contributes to off-target ZFN cleavage and suggest strategies for the improvement of future ZFN design...
- Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processingEleni P Mimitou
Department of Microbiology, Columbia University Medical Center, 701 West 168th Street, New York, New York 10032, USA
Nature 455:770-4. 2008..Second, Exo1 and/or Sgs1 rapidly process this intermediate to generate extensive tracts of single-stranded DNA that serve as substrate for Rad51...
- A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiationJing Pan
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
Cell 144:719-31. 2011..This map illuminates the occurrence of DSBs in repetitive DNA elements, repair of which can lead to chromosomal rearrangements. We also discuss implications for evolutionary dynamics of recombination hot spots...
- An unbiased genome-wide analysis of zinc-finger nuclease specificityRichard Gabriel
Department of Translational Oncology, National Center for Tumor Diseases NCT and German Cancer Research Center DKFZ, Heidelberg, Germany
Nat Biotechnol 29:816-23. 2011..Comprehensive mapping of ZFN activity in vivo will facilitate the broad application of these reagents in translational research...
- Delineation of joint molecule resolution pathways in meiosis identifies a crossover-specific resolvaseKseniya Zakharyevich
Department of Microbiology, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA
Cell 149:334-47. 2012..Thus, Sgs1, Exo1, and MutLγ together define a previously undescribed meiotic JM resolution pathway that produces the majority of crossovers in budding yeast and, by inference, in mammals...
- Bidirectional resection of DNA double-strand breaks by Mre11 and Exo1Valerie Garcia
Genome Damage and Stability Centre, The University of Sussex, Brighton, BN1 9RQ, UK
Nature 479:241-4. 2011..Mre11 exonuclease activity also confers resistance to DNA damage in cycling cells, suggesting that Mre11-catalysed resection may be a general feature of various DNA repair pathways...
- Spo11-accessory proteins link double-strand break sites to the chromosome axis in early meiotic recombinationSilvia Panizza
Department of Chromosome Biology, Max F Perutz Laboratories, University of Vienna, Dr Bohr Gasse 1, A 1030 Vienna, Austria
Cell 146:372-83. 2011..Loss of Rec114, Mer2, and Mei4 binding correlates with loss of DSBs. Our results strongly suggest that hotspot sequences become tethered to axis sites by the DSB machinery prior to DSB formation...
- BLM helicase ortholog Sgs1 is a central regulator of meiotic recombination intermediate metabolismArnaud De Muyt
Laboratory of Biochemistry and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
Mol Cell 46:43-53. 2012..We conclude that Sgs1 is a primary regulator of recombination pathway choice during meiosis and suggest a similar function in the mitotic cell cycle...
- ATM controls meiotic double-strand-break formationJulian Lange
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA
Nature 479:237-40. 2011..Our findings explain previously puzzling phenotypes of Atm-null mice and provide a molecular basis for the gonadal dysgenesis observed in ataxia telangiectasia, the human syndrome caused by ATM deficiency...
- Enhancing gene targeting with designed zinc finger nucleasesMarina Bibikova
Department of Biochemistry, University of Utah School of Medicine, 20 North 1900 East, Salt Lake City, UT 84132 3201, USA
Science 300:764. 2003
- The mouse Spo11 gene is required for meiotic chromosome synapsisP J Romanienko
Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health 20892, Bethesda, MD, USA
Mol Cell 6:975-87. 2000..We speculate that there is an additional role for Spo11, after it generates DSBs, in synapsis...
- Identification and characterization of a family of mammalian methyl-CpG binding proteinsB Hendrich
Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland
Mol Cell Biol 18:6538-47. 1998..The data demonstrate that MBD2 and MBD4 bind specifically to methyl-CpG in vitro and in vivo and are therefore likely to be mediators of the biological consequences of the methylation signal...
- Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombinationMONICA P COLAIACOVO
Department of Developmental Biology, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
Dev Cell 5:463-74. 2003..Our data also suggest that early prophase barriers to utilization of sister chromatids as repair templates do not depend on central region assembly...
- Mechanism and control of meiotic recombination initiationS Keeney
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, and Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
Curr Top Dev Biol 52:1-53. 2001....
- Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11F Baudat
Cell Biology Program Memorial Sloan Kettering Cancer Center New York, NY 10021, USA
Mol Cell 6:989-98. 2000..Our results also support the view that mammalian checkpoint responses to meiotic recombination and/or synapsis defects are sexually dimorphic...
- Mechanism of Holliday junction resolution by the human GEN1 proteinUlrich Rass
London Research Institute, Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts, United Kingdom
Genes Dev 24:1559-69. 2010..However, the unique feature of GEN1 that distinguishes it from other Rad2/XPG nucleases is its ability to dimerize on HJs. This functional adaptation provides the two symmetrically aligned active sites required for HJ resolution...
- The Mre11 complex is required for repair of hairpin-capped double-strand breaks and prevention of chromosome rearrangementsKirill S Lobachev
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
Cell 108:183-93. 2002..Our results suggest an additional role for the Mre11 complex in maintaining genome stability...
- The COMPASS subunit Spp1 links histone methylation to initiation of meiotic recombinationLaurent Acquaviva
Marseille Cancer Research Center, U1068 Inserm, UMR7258 CNRS, Aix Marseille University, Institut Paoli Calmettes, Marseille 13009, France
Science 339:215-8. 2013..Thus, by interacting with H3K4me3 and Mer2, Spp1 promotes recruitment of potential meiotic DSB sites to the chromosomal axis, allowing Spo11 cleavage at nearby nucleosome-depleted regions...
- Caenorhabditis elegans HIM-18/SLX-4 interacts with SLX-1 and XPF-1 and maintains genomic integrity in the germline by processing recombination intermediatesTakamune T Saito
Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
PLoS Genet 5:e1000735. 2009..elegans BLM homolog. We propose that HIM-18 facilitates processing of HR intermediates resulting from replication fork collapse and programmed meiotic DSBs in the C. elegans germline...
- Highly efficient endogenous human gene correction using designed zinc-finger nucleasesFyodor D Urnov
Sangamo BioSciences, Inc, Pt Richmond Tech Center 501, Canal Blvd, Suite A100 Richmond, California 94804, USA
Nature 435:646-51. 2005..We observe comparably high frequencies in human T cells, raising the possibility of strategies based on zinc-finger nucleases for the treatment of disease...
- The structure-specific endonuclease Mus81-Eme1 promotes conversion of interstrand DNA crosslinks into double-strands breaksKatsuhiro Hanada
Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
EMBO J 25:4921-32. 2006..We propose that Mus81-Eme1- and Rad54-mediated homologous recombination are involved in the same DNA replication-dependent ICL repair pathway...
- Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX complex or Sae2Eleni P Mimitou
Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY, USA
EMBO J 29:3358-69. 2010..Finally, we show that the presence of Ku exacerbates DNA end-processing defects established in the sae2Δ sgs1Δ mutant, leading to its lethality...
- Saccharomyces Ku70, mre11/rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damageS E Lee
Rosenstiel Center, Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
Cell 94:399-409. 1998..We suggest that escape from the DNA damage-induced G2/M checkpoint depends on the extent of ssDNA created at broken chromosome ends. RPA appears to play a key intermediate step in this adaptation...
- Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double-strand break endsZhu Zhu
Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Cell 134:981-94. 2008..These results provide important insights into the early steps of DSB repair in eukaryotes...
- Engineering domain fusion chimeras from I-OnuI family LAGLIDADG homing endonucleasesSarah Baxter
Department of Immunology, University of Washington, Seattle, WA 98195, USA
Nucleic Acids Res 40:7985-8000. 2012..This method will be useful for creating large numbers of chimeric LHEs for genome engineering applications...
- Mre11-Rad50-Xrs2 and Sae2 promote 5' strand resection of DNA double-strand breaksMatthew L Nicolette
The Howard Hughes Medical Institute and the Department of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, Texas, USA
Nat Struct Mol Biol 17:1478-85. 2010..This stimulation is mainly caused by cooperative binding of DNA substrates by Exo1, MRX and Sae2. This work establishes the direct role of MRX and Sae2 in promoting the resection of 5' strands in DNA DSB repair...
- DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2Petr Cejka
Department of Microbiology, University of California, Davis, Davis, California 95616 8665, USA
Nature 467:112-6. 2010..We suggest that Top3-Rmi1 and MRX are important for recruitment of the Sgs1-Dna2 complex to DSBs. Our experiments provide a mechanistic framework for understanding the initial steps of recombinational DNA repair in eukaryotes...
- Ctp1 is a cell-cycle-regulated protein that functions with Mre11 complex to control double-strand break repair by homologous recombinationOliver Limbo
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Mol Cell 28:134-46. 2007..Transcription of ctp1(+) is periodic during the cell cycle, with the onset of its expression coinciding with the start of DNA replication. These data suggest that regulation of Ctp1 underlies cell-cycle control of HR...
- Disjunction of homologous chromosomes in meiosis I depends on proteolytic cleavage of the meiotic cohesin Rec8 by separinS B Buonomo
Research Institute of Molecular Pathology, University of Vienna, Austria
Cell 103:387-98. 2000..We show here that cleavage of Rec8 by separin at one of two different sites is necessary for the resolution of chiasmata and the disjunction of homologous chromosomes during meiosis...
- SPO11 is required for sex-body formation, and Spo11 heterozygosity rescues the prophase arrest of Atm-/- spermatocytesMarina A Bellani
Genetics and Biochemistry Branch, NIDDK, NIH, Bethesda, MD 20892, USA
J Cell Sci 118:3233-45. 2005....
- Endonuclease G regulates budding yeast life and deathSabrina Buttner
Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
Mol Cell 25:233-46. 2007..Deletion of NUC1 diminishes apoptotic death when mitochondrial respiration is increased but enhances necrotic death when oxidative phosphorylation is repressed, pointing to dual--lethal and vital--roles for EndoG...
- Drosophila MUS312 and the vertebrate ortholog BTBD12 interact with DNA structure-specific endonucleases in DNA repair and recombinationSabrina L Andersen
Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA
Mol Cell 35:128-35. 2009..Genetic and biochemical evidence described here and in related papers suggest that MUS312 and BTBD12 direct Holliday junction resolution by at least two distinct endonucleases in different recombination and repair contexts...
- SLX-1 is required for maintaining genomic integrity and promoting meiotic noncrossovers in the Caenorhabditis elegans germlineTakamune T Saito
Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
PLoS Genet 8:e1002888. 2012..Moreover, we hypothesize that SLX-1 regulates the crossover landscape during meiosis by acting as a noncrossover-promoting factor in a subset of DSBs...
- Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapyGeorge Silva
Cellectis Genome Surgery, 102 Avenue Gaston Roussel, Romainville Cedex, France
Curr Gene Ther 11:11-27. 2011..Here, we review these alternative approaches, with a special emphasis on meganucleases, a family of naturally occurring rare-cutting endonucleases, and speculate on their current and future potential...
- Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaksEun Yong Shim
Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
EMBO J 29:3370-80. 2010..However, Mre11 nuclease activity is essential for resection in the absence of extensive resection enzymes. The results provide new insights into how MRX catalyses end resection and recombination initiation...
- Endonucleolytic processing of covalent protein-linked DNA double-strand breaksMatthew J Neale
Molecular Biology Programs, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Nature 436:1053-7. 2005..Our findings suggest a general mechanism for repair of protein-linked DSBs...
- A DNA damage response pathway controlled by Tel1 and the Mre11 complexT Usui
Department of Biology, Graduate School of Science, Osaka University, Toyonaka, 560 0043, Osaka, Japan
Mol Cell 7:1255-66. 2001..These findings demonstrate that the diverse functions of the Mre11 complex in the cellular DNA damage response are conserved in mammals and yeast...
- The nuclease activity of Mre11 is required for meiosis but not for mating type switching, end joining, or telomere maintenanceS Moreau
Institute of Cancer Research and Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
Mol Cell Biol 19:556-66. 1999..We propose that in mitotic cells DSBs can be processed by other nucleases that are partially redundant with Mre11, but these activities are unable to process Spo11-bound DSBs in meiotic cells...
- The Mre11:Rad50 structure shows an ATP-dependent molecular clamp in DNA double-strand break repairKatja Lammens
Center for Integrated Protein Science Munich, Ludwig Maximilians University Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
Cell 145:54-66. 2011..The results suggest that MR is an ATP-controlled transient molecular clamp at DNA double-strand breaks...
- The axial element protein HTP-3 promotes cohesin loading and meiotic axis assembly in C. elegans to implement the meiotic program of chromosome segregationAaron F Severson
Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
Genes Dev 23:1763-78. 2009..Premature equational sister separation in rec8 mutants of other organisms suggests the involvement of multiple REC-8 paralogs, which may have masked a conserved requirement for cohesin in AE assembly...
- DNA damage signaling recruits the Rtt107-Slx4 scaffolds via Dpb11 to mediate replication stress responsePatrice Y Ohouo
Graduate Program in Biochemistry, Molecular and Cell Biology, Weill Institute for Cell and Molecular Biology, Cornell University, 339 Weill Hall, Ithaca, New York 14853 7202, USA
Mol Cell 39:300-6. 2010..Our results unveil how Mec1 regulates the Slx4 and Rtt107 scaffolds and establish a mechanistic link between DNA damage signaling and fork repair...
- Structural, functional and evolutionary relationships between homing endonucleases and proteins from their host organismsGregory K Taylor
Graduate Program in Molecular and Cellular Biology, University of Washington and Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N A3 025, Seattle, WA 90109, USA
Nucleic Acids Res 40:5189-200. 2012..This review summarizes the results of structural studies of HEs and corresponding proteins from host organisms that have illustrated the manner in which these factors are related...
- A synaptonemal complex protein promotes homology-independent centromere couplingTomomi Tsubouchi
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
Science 308:870-3. 2005..Regions of synaptonemal complex assembled early in meiosis are often centromere-associated. We propose that centromere coupling facilitates homolog pairing and promotes synapsis initiation...
- Functional interplay of the Mre11 nuclease and Ku in the response to replication-associated DNA damageSteven S Foster
Laboratory of Chromosome Biology, Memorial Sloan Kettering Cancer Center, 1275 York Ave, RRL 901C, New York, NY 10021, USA
Mol Cell Biol 31:4379-89. 2011..Collectively, the data define a nonhomologous end joining (NHEJ)-independent, S-phase-specific function of the Ku heterodimer...
- Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleasesInes Fonfara
Institut fur Biochemie, Justus Liebig Universitat Giessen, Heinrich Buff Ring 58, D 35392, Giessen, Germany
Nucleic Acids Res 40:847-60. 2012....
- Single-strand nicks induce homologous recombination with less toxicity than double-strand breaks using an AAV vector templateMichael J Metzger
Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Nucleic Acids Res 39:926-35. 2011....
- Crystal structure of the Mus81-Eme1 complexJeong Ho Chang
National Creative Initiatives for Structural Biology and Department of Life Science, Pohang University of Science and Technology, Pohang, Kyungbook 790 784, South Korea
Genes Dev 22:1093-106. 2008....
- Systematic genetic analysis with ordered arrays of yeast deletion mutantsA H Tong
Banting and Best Department of Medical Research, University of Toronto, Toronto ON, Canada M5G 1L6
Science 294:2364-8. 2001..Systematic application of this approach should produce a global map of gene function...
- Uptake of transforming DNA in Gram-positive bacteria: a view from Streptococcus pneumoniaeMathieu Berge
Laboratoire de Microbiologie et Genetique Moleculaire, UMR 5100 CNRS Université Paul, Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France
Mol Microbiol 45:411-21. 2002..Finally, inactivation of dprA had no effect on the internalization of DNA, indicating that DprA is required at a later stage in transformation...
- Endonuclease G is an apoptotic DNase when released from mitochondriaL Y Li
Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75390, USA
Nature 412:95-9. 2001..Once released from mitochondria, endoG cleaves chromatin DNA into nucleosomal fragments independently of caspases. Therefore, endoG represents a caspase-independent apoptotic pathway initiated from the mitochondria...
- Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiaeShohreh Maleki
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY 10021, USA
Chromosoma 116:471-86. 2007..These studies also suggest that an essential function of Rec102 and Rec104 is to connect Mei4 and Rec114 to Spo11...
- The generation of proper constitutive G-tails on yeast telomeres is dependent on the MRX complexMichel Larrivée
Departement De Microbiologie et Infectiologie, Groupe ARN RNA Group, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, J1H 5N4 Canada
Genes Dev 18:1391-6. 2004..Thus, G-tails are present on yeast chromosomes throughout the cell cycle and the MRX complex is required for their normal establishment...
- The junction-resolving enzymesD M Lilley
CRC Nucleic Acid Structure Research Group, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, UK
Nat Rev Mol Cell Biol 2:433-43. 2001..This poses an intriguing challenge in molecular recognition on a relatively large scale...
- Human MUS81 complexes stimulate flap endonuclease 1Yong Keol Shin
Department of Biological Sciences, Center for DNA Replication and Genome Instability, Korea Advanced Institute of Science and Technology, Daejeon, Korea
FEBS J 279:2412-30. 2012..Thus, the mammalian MUS81 complexes and FEN1 collaborate to remove the various flap structures that arise during many DNA transactions, including Okazaki fragment processing...
- Distinct roles of two separable in vitro activities of yeast Mre11 in mitotic and meiotic recombinationM Furuse
Cellular and Molecular Biology Laboratory, The Institute of Physical and Chemical Research RIKEN, Wako Shi, Saitama 351 0198, Japan
EMBO J 17:6412-25. 1998....
- A central coupler for recombination initiation linking chromosome architecture to S phase checkpointTomoichiro Miyoshi
Department of Life Sciences, The University of Tokyo, Komaba 3 8 1, Meguro ku, Tokyo 153 8902, Japan
Mol Cell 47:722-33. 2012..These data lead us to propose a mechanism by which Mde2 functions as a recombination initiation mediator to tether axes and loops, in liaison with the meiotic replication checkpoint...
- Crystal structure of the Mre11-Rad50-ATPγS complex: understanding the interplay between Mre11 and Rad50Hye Seong Lim
Department of Life Science, Pohang University of Science and Technology, Pohang, South Korea
Genes Dev 25:1091-104. 2011..Our structural and biochemical data provide insights into understanding the interplay between Mre11 and Rad50 to facilitate efficient DNA damage repair...
- EENdb: a database and knowledge base of ZFNs and TALENs for endonuclease engineeringAn Xiao
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, State Key Laboratory of Protein and Plant Gene Research, Center for Bioinformatics, College of Life Sciences, Peking University, Beijing 100871, China
Nucleic Acids Res 41:D415-22. 2013..These studies may help to extract in-depth properties and common rules regarding ZFN or TALEN efficiency through comparison of the known ZFNs or TALENs...
- The Rad50 coiled-coil domain is indispensable for Mre11 complex functionsMarcel Hohl
Laboratory of Chromosome Biology, Memorial Sloan Kettering Cancer Center, New York, New York, USA
Nat Struct Mol Biol 18:1124-31. 2011..These data show that functions of Mre11 complex are integrated by the coiled coils of Rad50...
- Distinct DNA-damage-dependent and -independent responses drive the loss of oocytes in recombination-defective mouse mutantsMonica Di Giacomo
Molecular Biology Program, Memorial Sloan Kettering Cancer Center and Weill Graduate School of Medical Sciences of Cornell University, 1275 York Avenue, New York, NY 10021, USA
Proc Natl Acad Sci U S A 102:737-42. 2005..The absence of ATM caused defects in folliculogenesis that were similar to those in Dmc1 mutants and that could be suppressed by Spo11 mutation, implying that oocyte death in Atm-deficient animals is a response to defective DSB repair...
- Three structure-selective endonucleases are essential in the absence of BLM helicase in DrosophilaSabrina L Andersen
Curriculum in Genetics and Molecular Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
PLoS Genet 7:e1002315. 2011..Our studies of synthetic lethality provide insights into the multiple functions of DmBLM and how various endonucleases may function when DmBLM is absent...
- DNA nicks promote efficient and safe targeted gene correctionLuther Davis
Department of Immunology, University of Washington School of Medicine, Seattle, Washington, United States of America
PLoS ONE 6:e23981. 2011..42±0.03%, respectively). Thus nicks initiate efficient gene correction, with limited local mutagenesis. These results have clear therapeutic implications, and should inform future design of meganucleases for targeted gene correction...
- DNA bending and a flip-out mechanism for base excision by the helix-hairpin-helix DNA glycosylase, Escherichia coli AlkAT Hollis
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
EMBO J 19:758-66. 2000..The structure of the AlkA-DNA complex offers the first glimpse of a helix-hairpin-helix (HhH) glycosylase complexed to DNA. Modeling studies suggest that other HhH glycosylases can bind to DNA in a similar manner...
- Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombinationHiroyuki Sasanuma
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3 8 1, Meguro ku, Tokyo 153 8902, Japan
Genes Dev 22:398-410. 2008..We thus propose that Cdc7, in concert with CDK, regulates Spo11 loading to DSB sites via Mer2 phosphorylation...
- Mus81-Eme1 are essential components of a Holliday junction resolvaseM N Boddy
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Cell 107:537-48. 2001..The mus81 meiotic defect is rescued by expression of a bacterial Holliday junction resolvase. These findings constitute strong evidence that Mus81 and Eme1 are subunits of a nuclear Holliday junction resolvase...
- Distinct properties of the XY pseudoautosomal region crucial for male meiosisLiisa Kauppi
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
Science 331:916-20. 2011..Our findings uncover specific mechanisms that surmount the unique challenges of X-Y recombination...
- Cleavage mechanism of human Mus81-Eme1 acting on Holliday-junction structuresEwan R Taylor
Departments of Molecular Biology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 105:3757-62. 2008....
- ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repairGareth J Williams
Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
Nat Struct Mol Biol 18:423-31. 2011....
- Saccharomyces cerevisiae checkpoint genes MEC1, RAD17 and RAD24 are required for normal meiotic recombination partner choiceJ M Grushcow
Department of Radiation, University of Chicago, Chicago, Illinois 60637, USA
Genetics 153:607-20. 1999..These observations support the suggestion that MEC1, RAD17, and RAD24, in addition to their proposed monitoring function, act to promote normal meiotic recombination...
- Progression of meiotic DNA replication is modulated by interchromosomal interaction proteins, negatively by Spo11p and positively by Rec8pR S Cha
Department of Molecular Biology, Harvard University, Cambridge, Massachusetts 02138 USA
Genes Dev 14:493-503. 2000..Roles for Rec8 and/or Spo11 in progression through other stages of meiosis are also revealed...
- Homing endonucleases: from basics to therapeutic applicationsMaria J Marcaida
Macromolecular Crystallography Group, Structural Biology and Biocomputing Programme, Spanish National Cancer Research Centre CNIO, c Melchor Fdez Almagro 3, 28029 Madrid, Spain
Cell Mol Life Sci 67:727-48. 2010..The use of tailored HEs opens up new possibilities for gene therapy in patients with monogenic diseases that can be treated ex vivo. This review provides an overview of recent advances in this field...
- Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequencesJia Lin Ma
Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
Mol Cell Biol 23:8820-8. 2003..The increased gamma ray sensitivity of rad1Delta rad52Delta yku70Delta strains compared to rad52Delta yku70Delta strains suggests that MMEJ also contributes to the repair of DSBs induced by ionizing radiation...
- Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82A A Mahdi
Department of Genetics, University of Nottingham Queens Medical Centre, UK
J Mol Biol 257:561-73. 1996..Multicopy plasmids expressing RusA alone make ruvA, ruvB, and ruvC mutants resistant to UV light. Suppression depends critically on RecG...
- Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axesVérane Sommermeyer
Institut Curie, Centre de Recherche, 75248 Paris, France
Mol Cell 49:43-54. 2013..This paper provides the molecular mechanism linking DSB sequences to chromosome axes and explains why H3K4 methylation is important for meiotic recombination...
- Mitochondrial release of AIF and EndoG requires caspase activation downstream of Bax/Bak-mediated permeabilizationDamien Arnoult
Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
EMBO J 22:4385-99. 2003..Thus EndoG and AIF seem to define a 'caspase-dependent' mitochondria-initiated apoptotic DNA degradation pathway that is conserved between mammals and nematodes...
- The Mre11 protein interacts with both Rad50 and the HerA bipolar helicase and is recruited to DNA following gamma irradiation in the archaeon Sulfolobus acidocaldariusAchim Quaiser
Univ Paris Sud, Institut de Genetique et Microbiologie, CNRS UMR 8621, Batiment 409, F 91405 Orsay cedex, France
BMC Mol Biol 9:25. 2008..Since genetic tools for hyperthermophilic archaea are just emerging, we utilized immuno-detection approaches to get the first in vivo data on the role(s) of these proteins in the hyperthermophilic crenarchaeon Sulfolobus acidocaldarius...
- Gamma radiation increases endonuclease-dependent L1 retrotransposition in a cultured cell assayEvan A Farkash
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Nucleic Acids Res 34:1196-204. 2006..The mobilization of L1 in irradiated cells potentially contributes to genomic instability and could be a driving force for secondary mutations in patients undergoing radiation therapy...
- DNA interstrand crosslink repair during G1 involves nucleotide excision repair and DNA polymerase zetaSovan Sarkar
Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
EMBO J 25:1285-94. 2006..We show that this combination of NER and TLS is the only pathway of ICL repair available to the cell in G1 phase and is essential for viability in the presence of DNA crosslinks...
- Yeast nucleotide excision repair proteins Rad2 and Rad4 interact with RNA polymerase II basal transcription factor b (TFIIH)A J Bardwell
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235
Mol Cell Biol 14:3569-76. 1994..Furthermore, the loading of factor b (or such intermediates) onto promoters during transcription initiation provides a mechanism for the preferential targeting of repair proteins to actively transcribing genes...
- S. cerevisiae Tel1p and Mre11p are required for normal levels of Est1p and Est2p telomere associationLara K Goudsouzian
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
Mol Cell 24:603-10. 2006..These data argue that Mre11p and Tel1p affect telomere length by promoting telomerase recruitment to telomeres, whereas Mec1p has only a minor role in telomerase recruitment in a TEL1 cell...
- Distinct roles of Mus81, Yen1, Slx1-Slx4, and Rad1 nucleases in the repair of replication-born double-strand breaks by sister chromatid exchangeSandra Muñoz-Galván
Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Universidad de Sevilla CSIC, Seville, Spain
Mol Cell Biol 32:1592-603. 2012..These unique effects, which have not been detected in other studies unless double mutant combinations were used, indicate the formation of distinct alternatives for the repair of replication-born DSBs that require specific SSEs...
- Rec8 guides canonical Spo11 distribution along yeast meiotic chromosomesKazuto Kugou
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Tokyo 153 8902, Japan
Mol Biol Cell 20:3064-76. 2009..These observations suggest that Rec8 would prearrange the distribution of Spo11 along chromosomes and will provide clues to understanding temporal and spatial regulation of DSB formation...
- Divalent metal ion differentially regulates the sequential nicking reactions of the GIY-YIG homing endonuclease I-BmoIBenjamin P Kleinstiver
Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada
PLoS ONE 6:e23804. 2011..Collectively, our data implies mechanistic roles for divalent metal ion and substrate bases, suggesting that divalent metal ion facilitates the re-positioning of the GIY-YIG nuclease domain between sequential nicking reactions...
- Activity, specificity and structure of I-Bth0305I: a representative of a new homing endonuclease familyGregory K Taylor
Graduate Program in Molecular and Cellular Biology and the Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98019, USA
Nucleic Acids Res 39:9705-19. 2011..The domain architecture and DNA recognition profile displayed by I-Bth0305I, which is the prototype of a homing lineage that we term the 'EDxHD' family, are distinct from previously characterized homing endonucleases...
- Sae2 is an endonuclease that processes hairpin DNA cooperatively with the Mre11/Rad50/Xrs2 complexBettina M Lengsfeld
Department of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX 78712, USA
Mol Cell 28:638-51. 2007....
- Genetic and biochemical characterization of MbeA, the relaxase involved in plasmid ColE1 conjugative mobilizationAthanasia Varsaki
Sector of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 451 10 Ioannina, Greece
Mol Microbiol 48:481-93. 2003..Thus, MbeA is a variant of the common relaxase theme with a HEN signature motif, which has to be added to the canonical three histidine motif of previously reported relaxases...
- ATP driven structural changes of the bacterial Mre11:Rad50 catalytic head complexCarolin Möckel
Gene Center and Department of Biochemistry, Ludwig Maximilians University Munich, Feodor Lynen Strasse 25, 81377, Munich, Germany
Nucleic Acids Res 40:914-27. 2012..Our results suggest a possible mechanism for ATP-dependent DNA tethering and DSB processing by MR...
- Adaptation of intronic homing endonuclease for successful horizontal transmissionSayuri Kurokawa
Graduate School of Engineering, Department of Environmental Systems Engineering, Kochi University of Technology KUT, Kochi, Japan
FEBS J 272:2487-96. 2005....
- Genetic and structural characterization of endA. A membrane-bound nuclease required for transformation of Streptococcus pneumoniaeA Puyet
Department of Biology, Brookhaven National Laboratory, Upton, NY 11973
J Mol Biol 213:727-38. 1990..Neither of these upstream genes, nor the downstream gene, are essential for either cell viability or transformability...
- Atomic structure of the Serratia marcescens endonuclease at 1.1 A resolution and the enzyme reaction mechanismS V Shlyapnikov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str 32, Moscow 117984, Russia
Acta Crystallogr D Biol Crystallogr 56:567-72. 2000..marcescens nuclease structure, the functional characteristics of the natural and mutational forms of the enzyme and consideration of its structural analogy with homing endo-nuclease I-PpoI...
- Important role of the nucleotide excision repair pathway in Mycobacterium smegmatis in conferring protection against commonly encountered DNA-damaging agentsKrishna Kurthkoti
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India
Microbiology 154:2776-85. 2008..Taken together with previous observations on NER-deficient M. tuberculosis, these results suggest that NER is an important DNA repair pathway in mycobacteria...
- Nucleases of the metallo-beta-lactamase family and their role in DNA and RNA metabolismZbigniew Dominski
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Crit Rev Biochem Mol Biol 42:67-93. 2007..This article reviews the cellular roles of nucleases of the metallo-beta-lactamase family and the recent advances in studying these proteins...
- The hSNM1 protein is a DNA 5'-exonucleaseJames Hejna
Oregon Health and Science University, Department of Molecular and Medical Genetics, 3181 SW Sam Jackson Park Road, Mail Code L103, Portland, OR 97239 3098, USA
Nucleic Acids Res 35:6115-23. 2007..The exonuclease activity requires the conserved beta-lactamase domain; site-directed mutagenesis of a conserved aspartate inactivates the exonuclease...
- Stimulation of homology-directed gene targeting at an endogenous human locus by a nicking endonucleaseGijsbert P van Nierop
Virus and Stem Cell Biology Laboratory, Department of Molecular Cell Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands
Nucleic Acids Res 37:5725-36. 2009..Finally, harnessing the cellular HR pathway through Rep-mediated nicking expands the range of strategies that make use of AAV elements to bring about stable genetic modification of human cells...
- Mre11 nuclease activity and Ctp1 regulate Chk1 activation by Rad3ATR and Tel1ATM checkpoint kinases at double-strand breaksOliver Limbo
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
Mol Cell Biol 31:573-83. 2011....
- Synapsis-dependent and -independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegansAmy J MacQueen
Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305 5329, USA
Genes Dev 16:2428-42. 2002....
- Lethal anemia caused by interferon-beta produced in mouse embryos carrying undigested DNAHideyuki Yoshida
Department of Genetics, Osaka University Medical School, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
Nat Immunol 6:49-56. 2005....
- BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structuresY Wang
Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
Genes Dev 14:927-39. 2000..Collectively, these results suggest that BRCA1 may function as a coordinator of multiple activities required for maintenance of genomic integrity during the process of DNA replication and point to a central role for BRCA1 in DNA repair...
- Investigation of the substrate spectrum of the human mismatch-specific DNA N-glycosylase MED1 (MBD4): fundamental role of the catalytic domainF Petronzelli
Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA
J Cell Physiol 185:473-80. 2000..This suggests that the catalytic domain is fundamental, and the 5-methylcytosine binding domain dispensable, in determining the substrate spectrum of MED1...
- Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleasesC Ban
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
EMBO J 17:1526-34. 1998..With sequence homology to Sau3AI and structural similarity to PvuII endonuclease, MutH is clearly related to these enzymes by divergent evolution, and this suggests that type II restriction endonucleases evolved from a common ancestor...
- Recombinational DNA double-strand breaks in mice precede synapsisS K Mahadevaiah
Division of Developmental Genetics, National Institute for Medical Research, London, UK
Nat Genet 27:271-6. 2001..Loss of gamma-H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'...
- NOVEL HUMAN GENE SPECIFIC REAGENTS FROM HOMING ENDOSRaymond Monnat; Fiscal Year: 2005..The availability of human GSR's may also allow modification of altered human genes responsible for specific diseases or genes of pathogenic microorganisms including bacteria and viruses, parasites, etc. ..
- Checkpoint Signaling and Repair of UV Damage to Human DNAYue Zou; Fiscal Year: 2007..abstract_text> ..
- STRUCTURAL STUDIES OF INTRON ENCODED ENDONUCLEASESPatrick Van Roey; Fiscal Year: 2001..This will allow the comparison of representative examples of the two classes of enzymes that, although otherwise unrelated, carry out the same biological function. ..
- Postdoctoral Training in Renal Diseases and HypertensionBRUCE KONE; Fiscal Year: 2006..It is the goal of the Program that its graduates will contribute to a new generation of academic nephrologists and renal investigators equipped to address the challenging problems in renal diseases and hypertension ..
- Conference Proposal: FASEB Nucleic Acid EnzymesBarry Stoddard; Fiscal Year: 2006..unreadable] [unreadable] [unreadable]..
- Computations of Fold Changes in Evolution of ProteinsNick Grishin; Fiscal Year: 2006..Finally, we will perform in silico evolution of model proteins under functional constraints and compare the results to those deduced from the analysis of natural sequences and structures. ..
- A Genetic System Able to Screen for Active DNA DemethylationShuang yong Xu; Fiscal Year: 2006..In addition, knowledge of the mechanism of demethylation could be crucial in converting fully differentiated adult stem cells into totipotent stem cells. [unreadable] [unreadable] [unreadable]..
- Mechanisms of Mitotic RecombinationJEFF JOSEPH SEKELSKY; Fiscal Year: 2010..These studies will enhance our understanding of mechanisms of mitotic crossing over and the role that the important tumor suppressor protein BLM plays in preventing mitotic crossing over. ..
- Targeted gene repair of primary immune deficienciesAndrew Scharenberg; Fiscal Year: 2006..Overall, the work proposed here will build a foundation of materials and experience for the development of LHE's as general tools for targeted gene therapy of human primary immune deficiencies. ..
- DNA Packaging MotorsPhilip Serwer; Fiscal Year: 2005..Analysis of bacteriophage DNA packaging motors is expected to provide a basis for understanding the role of motors in disease, especially disease caused by a virus that has a DNA packaging motor. ..
- Engineering DNA nicking endonucleasesShuang yong Xu; Fiscal Year: 2006..Under Phase II funding, this isothermal amplification method (NEMDA) will be optimized and the sensitivity will be improved. Finally, we will investigate the potential of NEMDA for detections of pathogens and its use in bio-prospecting. ..
- Cytochrome P450 in Reperfusion InjuryRoberta Gottlieb; Fiscal Year: 2007..This proposal identifies cytochrome P450 monooxygenases as a previously underestimated factor in myocardial reperfusion injury, and establishes the basis for a novel therapeutic approach [unreadable] [unreadable]..
- DNA Mismatch and Double-Strand Break RepairMartin G Marinus; Fiscal Year: 2010..It also impacts the mechanism by which pathogenic bacteria become resistant to antibiotics and how this resistance is disseminated. ..
- INTEGRATED STUDY OF MUSCULOSKELETAL LOSS AND RESTORATIONEsther E Dupont Versteegden; Fiscal Year: 2010..Identifying these common pathways influencing muscle and bone integrity could lead to putative targets for pharmacologic or therapeutic interventions. ..
- Engineering enzymes for anti-tumor suicide gene therapyBarry L Stoddard; Fiscal Year: 2010..Our hypothesis is that decitabine should ultimately couple with engineered enzyme variants to yield improvements in the performance of dCK, due to the lack of this activity in non-cancerous tissues. ..
- Regulation of H/K-ATPase in Kidney and ColonBRUCE KONE; Fiscal Year: 2009..abstract_text> ..
- Combined Methods for Protein-Structure PredictionKevin Karplus; Fiscal Year: 2007....
- Regulation of L1 retrotranspositionEline Luning Prak; Fiscal Year: 2008..A better understanding of how L1 retrotransposition is regulated should yield important insights into L1 biology, mechanism and the potential of L1s to contribute to genomic instability in cancer. ..
- Genetic Recombination and Chromosome RearrangementsLORRAINE SYMINGTON; Fiscal Year: 2007..Much of the conference will be devoted to topics of concern both to the National Cancer Institute and to the Institute of Environmental and Health Sciences. [unreadable] [unreadable] [unreadable] [unreadable]..
- HUMAN OXIDATION RESISTANCE GENESMichael Volkert; Fiscal Year: 2007..abstract_text> ..
- DNA Damage Clusters: Repair in Mammalian CellsBetsy Sutherland; Fiscal Year: 2004..Further, knowledge of conditions affecting formation of clustered damages, and cellular paths for dealing with them could allow the design of more effective radiation therapies. ..
- Copy number control of the yeast plasmidMakkuni Jayaram; Fiscal Year: 2004..abstract_text> ..
- FUNCTION AND REGULATION OF THE E COLI AIDB GENESMichael Volkert; Fiscal Year: 2002..During the proposed funding period, I propose to characterize this factor further by genetic and biochemical means and to compare its induction mechanism with that of meAda. ..
- Assembly Visualized Routine Flourescence MicroscopyPhilip Serwer; Fiscal Year: 2008..Applications are anticipated in the analysis of the assembly of disease-causing viruses and determining pathways of oncogenesis. ..
- RETROTRANSPOSITION IN L1 TRANSGENIC MICEEline Luning Prak; Fiscal Year: 2004..abstract_text> ..
- ANALYSIS OF B-CELL RECEPTOR CALCIUM SIGNALINGAndrew Scharenberg; Fiscal Year: 2004..abstract_text> ..
- SCLERODERMA LUNG STUDYMaureen Mayes; Fiscal Year: 2003..abstract_text> ..
- OXYGEN INDUCED DNA DAMAGE, MUTATIONS AND CANCERLAWRENCE LOEB; Fiscal Year: 2003..Even a modest, 2-fold, decrease could increase the latent period before disease is manifested clinically and thus reduce cancer mortality. ..
- Genetic Recombination/Genomic Rearrangements ConferenceLORRAINE SYMINGTON; Fiscal Year: 2005..abstract_text> ..
- ROLE OF THE LCR IN HUMAN BETA-GLOBIN GENE REGULATIONKENNETH PETERSON; Fiscal Year: 2001..Specific Aim 3 will test the role of the LCR sequences in LCR complex formation and globin gene regulation using transgenic mice containing mutant beta-globin loci by applying FRET. ..
- DNA REPAIR DEFECT IN FANCONI ANEMIA, GROUP AMURIEL LAMBERT; Fiscal Year: 2008....
- Apoptosis in recombination-deficient meiocytesFrancesca Cole; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Chimeric Nucleases for Gene Targeting in DrosophilaDana Carroll; Fiscal Year: 2003..To test the generality of the method, it will be applied to a second Drosophila locus, the rosy (ry) gene. ..
- Bid-Mediated Cytochrome c Release from MitochondriaRoberta Gottlieb; Fiscal Year: 2007..These studies will elucidate the molecular mechanism by which BH3-only proteins cause the release of cytochrome c from mitochondria. [unreadable] [unreadable]..
- BRAIN TISSUE RESOURCE FOR NEUROPSYCHIATRIC RESEARCHFrancine Benes; Fiscal Year: 2002..Over the next five years, all of these changes will enhance the "brain bank" in its role as a vital national resource. ..
- Mechanisms of Double-strand Break Repair in YeastLORRAINE SYMINGTON; Fiscal Year: 2009..The last aim includes in vitro studies to identify preferred DNA substrates for Rad55:Rad57 binding and stabilization of the Rad51 nucleoprotein by Rad55:Rad57 in the presence or absence of Srs2. ..
- CORTICOLIMBIC SYSTEM AND SCHIZOPHRENIAFrancine Benes; Fiscal Year: 2002....
- The Werner Syndrome Protein in DNA Replication, Mutagenesis and Genomic StabilityLawrence A Loeb; Fiscal Year: 2010..All of the procedures have been established and are ongoing in our laboratory and can be completed within two years. ..
- MECHANISMS OF SITE-SPECIFIC RECOMBINATIONMakkuni Jayaram; Fiscal Year: 2009..Finally, they will set the stage for analyzing recombination in the context of high-order chromatin and native 2 micron circle physiology. ..
- NEUTROPHINS AND RETINAL DEVELOPMENTJanet Sparrow; Fiscal Year: 2001..The use of neurotrophins in conjunction with gene therapy or transplantation may also have potential for reversing retinal degenerative changes and lead to the restoration of vision. ..
- CREATING MUTANT ENZYMES FOR GENE THERAPY OF CANCERLAWRENCE LOEB; Fiscal Year: 2007..abstract_text> ..
- 12th International Workshop on Ataxia-Telangiectasia and ATMRichard Gatti; Fiscal Year: 2006..unreadable] [unreadable] [unreadable]..
- Nucleosomes Modulate DNA Interstrand Crosslink RepairMURIEL LAMBERT; Fiscal Year: 2005..abstract_text> ..
- Structural studies of blood coagulation proteinsBarry Stoddard; Fiscal Year: 2006..The third specific aim for this project is to determine the structure of the N-terminal, D'-D3 domain from von Willebrand Factor, alone and/or in complex with factor VIII heterodimer. ..
- Stimulating Gene Targeting with Zinc Finger NucleasesDana Carroll; Fiscal Year: 2004..To demonstrate the generality of this approach to gene targeting, it will be applied to another mouse locus, the Rpo2-1 gene. ..
- Cloning and Engineering of Nicking enzymesShuang yong Xu; Fiscal Year: 2004..abstract_text> ..
- A universal DNA endonucleaseShuang yong Xu; Fiscal Year: 2005..For isothermal applications of the universal nuclease at 50oC-70oC, the nuclease domain will be derived from thermophilic endonucleases. ..
- DNA Repair and Mutagenesis in S cerevisiaeElaine Sia; Fiscal Year: 2005..The experiments in this proposal are aimed at understanding the processes of mutagenesis and repair of mitochondrial DNA in yeast, however, these studies may lead to insights into similar processes in human mitochondrial DNA. ..
- Environmental Mutagen Society, 34th Annual MeetingLAWRENCE LOEB; Fiscal Year: 2003..Awards will be made to US speakers whose major area of interest is in carcinogenesis. ..
- Enhanced Capillary DNA SequencingPhilip Serwer; Fiscal Year: 2004..Each adaptation will help solve a current genomic problem, for example, either closing of a partially assembled genome sequence or performing high accuracy analysis of mutations. ..
- ANTITUMOR DRUGS AND INHIBITION OF INITIATION OF DNA REPLWILLIAM BURHANS; Fiscal Year: 2003..This project will explore these possibilities with a combined genetic and biochemical analysis of the effects of adozelesin and related drugs on initiation of DNA replication in mammalian cells. ..
- TRPM2 HTS Screening Assay Development(RMI)Andrew Scharenberg; Fiscal Year: 2005..Using the 96 well plate format, we will also evaluate the overall method's properties and suitability for HTS using a library of compounds of known bioactivity. ..
- A Multicomponent Anthrax Vaccine using Phage T4 DisplayVENIGALLA RAO; Fiscal Year: 2007..The duration of protection as well as pathological changes will be assessed. ..
- CORTICOLIMBIC ABNORMALITIES IN SCHIZOPHRENIC BRAINFrancine Benes; Fiscal Year: 2001....
- Improving Phenylbutyrate-based Anticancer TherapyASHOK BHAGWAT; Fiscal Year: 2005..This work will create the tools necessary for the monitoring of tumor cells in live animals when they are treated with PB as part of combination chemotherapy. ..
- LETHAL MUTAGENESIS OF HIV BY DEOXYNUCLEOSIDE ANALOGSLAWRENCE LOEB; Fiscal Year: 2002....
- Impaired Regulation of Muscle size with AgingESTHER DUPONT VERSTEEGDEN; Fiscal Year: 2002..Also, potential regulatory pathways and molecules involved in control of muscle size will be determined. ..
- Mechanisms of Nonhomologous Repair of Damaged DNASang Eun Lee; Fiscal Year: 2005..Furthermore, since DSB repair by NHEJ is remarkably conserved from yeast to humans, these studies will help to dissect the similar pathways in humans. ..
- Gonococcal Pilus Structure and FunctionH Seifert; Fiscal Year: 2008..These studies will provide new findings and insights into the structure and functions of the gonococcal pilus. ..
- VIRUS CHROMOSOME STRUCTURE: ROLE IN DEVELOPMENTMichael Feiss; Fiscal Year: 2005..We will also look at the ability of terminase to move DNA, and to pursue a structural analysis of functional domains of terminase. ..
- TARGETING ORIGINS OF REPLICATION WITH CANCER DRUGSWILLIAM BURHANS; Fiscal Year: 2004..abstract_text> ..
- Endonuclease dificiency in breast cancer progressionAlexei Basnakian; Fiscal Year: 2006..The next specific aim will be focused on functional studies to determine the role of EndoG in dedifferentiation and invasiveness of breast cancer cells in vitro (Specific Aim 2). ..