- SPO11 is required for sex-body formation, and Spo11 heterozygosity rescues the prophase arrest of Atm-/- spermatocytes
Marina A Bellani
Genetics and Biochemistry Branch, NIDDK, NIH, Bethesda, MD 20892, USA
J Cell Sci 118:3233-45. 2005
- Multiple insecticide resistance mechanisms involving metabolic changes and insensitive target sites selected in anopheline vectors of malaria in Sri Lanka
M Devika B Perera
Department of Zoology, Faculty of Science, University of Peradeniya, Peradeniya, Sri Lanka
Malar J 7:168. 2008
- Endonucleolytic processing of covalent protein-linked DNA double-strand breaks
Matthew J Neale
Molecular Biology Programs, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Nature 436:1053-7. 2005
..Our findings suggest a general mechanism for repair of protein-linked DSBs...
- cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases
Section for Molecular Signalling, Department of Cell and Molecular Biology, Lund University, P O Box 94, S 221 00 Lund, Sweden
J Biol Chem 272:27218-23. 1997
..Northern blot analysis revealed that monoglyceride lipase is ubiquitously expressed among tissues, with a transcript size of about 4 kilobases...
- Multiple biochemical and morphological factors underlie the production of methylketones in tomato trichomes
Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel
Plant Physiol 151:1952-64. 2009
..Furthermore, heterologous expression of MKS2 in Escherichia coli resulted in the production of methylketones in this host...
- Novel thermophilic and thermostable lipolytic enzymes from a Thailand hot spring metagenomic library
BIOTEC Central Research Unit, National Center for Genetic Engineering and Biotechnology, 113 National Science Park, Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand
J Biotechnol 133:42-9. 2008
..The isolated enzymes, are therefore, different from other known patatin-like phospholipases and esterases, which usually show no activity for substrates longer than C(10)...
- Cold-active esterase from Psychrobacter sp. Ant300: gene cloning, characterization, and the effects of Gly-->Pro substitution near the active site on its catalytic activity and stability
Laboratory of Microbial Biochemistry, Institute for Chemical Research, Kyoto University, Uji, Kyoto-fu, Kyoto 611-0011, Japan
Biochim Biophys Acta 1696:59-65. 2004
- Characterization and its potential application of two esterases derived from the arctic sediment metagenome
Jeong Ho Jeon
Marine Biotechnology Research Centre, Korean Ocean Research and Development Institute, Ansan, South Korea
Mar Biotechnol (NY) 11:307-16. 2009
..The esterases genes were overexpressed in Escherichia coli by co-expressing GroEL-GroES chaperonine, and the recombinant ..
- The lipid droplet enzyme Tgl1p hydrolyzes both steryl esters and triglycerides in the yeast, Saccharomyces cerevisiae
Institute of Molecular Biosciences, SFB Biomembrane Research Center, University of Graz, Schubertstr 1, A8010 Graz, Austria
Biochim Biophys Acta 1735:50-8. 2005
..cerevisiae. In a tgl1 deletion mutant, the mobilization of steryl esters in vivo was delayed, but not abolished, suggesting the existence of additional enzymes involved in steryl ester mobilization...
- Screening and its potential application of lipolytic activity from a marine environment: characterization of a novel esterase from Yarrowia lipolytica CL180
Jun Tae Kim
Marine Biotechnology Center, Korea Ocean Research and Development Institute, Ansan, South Korea
Appl Microbiol Biotechnol 74:820-8. 2007
..This study represents a novel esterase of type B1 carboxylesterase/lipase family from a marine isolate, showing a potential usage as a biocatalyst because of enantioselectivity toward racemic ofloxacin ester...
- Novel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
FEMS Microbiol Ecol 72:228-37. 2010
..Furthermore, the whole sequence of fosmid pFL18 containing FLS18C and FLS18D was shotgun sequenced, and a total of 26 ORFs on it were analyzed and annotated...
- Growth-phase-dependent expression of the lipolytic system of Acinetobacter calcoaceticus BD413: cloning of a gene encoding one of the esterases
R G Kok
Department of Microbiology, E C Slater Institute, BioCentrum Amsterdam, The Netherlands
J Gen Microbiol 139:2329-42. 1993
..0 kDa. The amino acid sequence of this protein shows homology to a number of lipolytic enzymes, most notably to esterases. Deletion of estA only partially abolished cell-bound esterase activity in A...
- Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from antarctic desert soil
Department of Biotechnology, University of the Western Cape, Cape Town, South Africa
Appl Environ Microbiol 75:4657-9. 2009
..The enzyme was active over a temperature range of 7 to 54 degrees C and at alkaline pH levels and is a potential candidate for industrial application...
- Structural reorganization and preorganization in enzyme active sites: comparisons of experimental and theoretically ideal active site geometries in the multistep serine esterase reaction cycle
Adam J T Smith
Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
J Am Chem Soc 130:15361-73. 2008
..The results provide a useful guiding principle for de novo design of enzymes with multistep mechanisms...
- Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics
Department of Entomology and BIO5, University of Arizona, Tucson, Arizona 85721, USA
Annu Rev Entomol 52:231-53. 2007
..e., cytochrome P450 monooxygenases (P450s), esterases, and glutathione-S-transferases (GSTs), are the focus of this review...
- Identification of two novel esterases from a marine metagenomic library derived from South China Sea
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230027, China
Appl Microbiol Biotechnol 80:615-25. 2008
..were isolated from a marine microbial metagenomic library by functional screening, and the corresponding esterases EstA and EstB were biochemically characterized...
- Plasma esterases and inflammation in ageing and frailty
Ruth E Hubbard
Department of Geriatric Medicine, School of Medicine, Cardiff University, 3rd Floor, Academic Centre, Llandough Hospital, Penarth, UK
Eur J Clin Pharmacol 64:895-900. 2008
b>Esterases are enzymes of drug metabolism known to be reduced in frail older people and during acute illness. The mechanism for this is unknown. The aim of this study was to examine esterase activity and inflammation in ageing and frailty.
- Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination
MONICA P COLAIACOVO
Department of Developmental Biology, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
Dev Cell 5:463-74. 2003
..Our data also suggest that early prophase barriers to utilization of sister chromatids as repair templates do not depend on central region assembly...
- Synapsis-dependent and -independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegans
Amy J MacQueen
Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305 5329, USA
Genes Dev 16:2428-42. 2002
- Enzymes from extremophiles
D C Demirjian
ThermoGen, Inc, Chicago, IL 60612, USA
Curr Opin Chem Biol 5:144-51. 2001
..This has also led to a greater understanding of stability factors involved in adaptation of these enzymes to their unusual environments...
- Functional expression and refolding of new alkaline esterase, EM2L8 from deep-sea sediment metagenome
Hyo Jung Park
Division of Biotechnology, The Catholic University of Korea, 43 1 Yokkok 2 dong, Wonmi gu, Bucheon, Gyeonggido 420 743, South Korea
Protein Expr Purif 52:340-7. 2007
..Moreover, it was found to have an optimum pH of 10-11, thus revealing that it is an alkaline enzyme. In this paper, the new esterase EM2L8 buried in a deep-sea sediment became known on the surface and was characterized biochemically...
- Isolation and characterization of cold-active family VIII esterases from an arctic soil metagenome
Eun Young Yu
Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, P O Box 107, Sinseongno 19, Yuseong, Daejeon 305 600, South Korea
Appl Microbiol Biotechnol 90:573-81. 2011
Functional screening for lipolytic enzymes at low temperatures resulted in the isolation of the novel cold-active esterases, EstM-N1 and EstM-N2, from a metagenomic DNA library of arctic soil samples...
- Genetically-determined polymorphism of nonspecific esterases and phosphoglucomutase in eight introduced breeds of the silkworm, Bombyx mori, raised in Bulgaria
Department of Developmental Biology, Section of Genetics, Faculty of Biology, University of Plovdiv, 4000 Plovdiv, Bulgaria
J Insect Sci 8:18. 2008
..Polymorphism of nonspecific esterases from pupal haemolymph was analyzed, as well as of phosphoglucomutase from different organs of larvae, pupae and ..
- The SGNH-hydrolase of Streptomyces coelicolor has (aryl)esterase and a true lipase activity
Laboratory of Molecular Genetics, Division of Molecular Biology, Ruder Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia
Biochimie 91:390-400. 2009
..coelicolor SGNH-hydrolase generated three distinct groups consisting of proteins from Actinomycetales, Ascomycota and Nematoda. At present it seems that these enzymes are most conserved among soil inhabiting organisms...
- The Lipase Engineering Database: a navigation and analysis tool for protein families
Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany
Nucleic Acids Res 31:319-21. 2003
..led.uni-stuttgart.de) integrates information on sequence, structure, and function of lipases, esterases, and related proteins...
- Activity of lipases and esterases towards tertiary alcohols: insights into structure-function relationships
Institute of Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstr. 16, 17487 Greifswald, Germany
Angew Chem Int Ed Engl 41:3211-3. 2002
- An exocellular protein from the oil-degrading microbe Acinetobacter venetianus RAG-1 enhances the emulsifying activity of the polymeric bioemulsifier emulsan
Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Science, Tel Aviv University, Tel Aviv 69978, Israel
Appl Environ Microbiol 69:2608-15. 2003
..Other proteins, including a His-tagged overexpressed esterase from the related organism Acinetobacter calcoaceticus BD4, showed no enhancement...
- Release of short chain fatty acids from cream lipids by commercial lipases and esterases
Laboratory of Industrial Microbiology and Biocatalysis, Faculty of Bioscience Engineering, Ghent University, Belgium
Biotechnol Lett 30:311-5. 2008
Lipases and esterases are frequently used in dairy production processes to enhance the buttery flavour of the end product...
- Risk of myocardial infarction associated with Gln/Arg 192 polymorphism in the human paraoxonase gene and diabetes mellitus. The REGICOR Investigators
Lipids and Cardiovascular Epidemiology Unit, Institut Municipal d Investigacio Medica, IMIM, Barcelona, Spain
Eur Heart J 21:33-8. 2000
- High-resolution structure of ybfF from Escherichia coli K12: a unique substrate-binding crevice generated by domain arrangement
Suk Youl Park
Department of Chemistry and Institute of Basic Sciences, Chonnam National University, 300 Yongbong Dong, Buk Gu, Gwangju 500 757, Korea
J Mol Biol 376:1426-37. 2008
b>Esterases are one of the most common enzymes and are involved in diverse cellular functions...
- Production of flavour compounds from fat during cheese ripening by action of lipases and esterases
Irma Verónica Wolf
Instituto de Lactología Industrial, Facultad de Ingenieria Quimica, Universidad Nacional del Litoral CONICET Consejo Nacional de Investigaciones Científicas y Técnicas, Santiago del Estero 2829, S3000AOM, Santa Fe, Argentina
Protein Pept Lett 16:1235-43. 2009
..Before starting with the specific study about formation of flavour compounds from milk fat during cheese ripening, a brief review of the technological aspects of cheese production is needed...
- Cloning and expression in Escherichia coli of an esterase-coding gene from the oil-degrading bacterium Acinetobacter calcoaceticus RAG-1
P G Reddy
Department of Microbiology, George S Wise Faculty of Life Sciences, Tel Aviv University, Israel
Gene 76:145-52. 1989
..The fact that the production of active esterase depended on the orientation of the est gene within the vector suggested that transcription proceeded from the tet promoter in pBR322...
- In vitro stability of methylmethacrylic acid, TEGDMA and HEMA exposed to esterases
Walther Straub Institute of Pharmacology and Toxicology, Ludwig Maximilians University of Munich, 80336 Munich, Germany
Dent Mater 25:1044-9. 2009
- Characterization of a novel thermostable esterase from Thermus scotoductus SA-01: evidence of a new family of lipolytic esterases
Erika M du Plessis
CSIR Biosciences, Box 395, Pretoria, 0001, Gauteng, South Africa
Curr Microbiol 60:248-53. 2010
..Sequence analysis showed that both proteins contained the G-X-S-X-G signature sequence motif present in most esterases and lipases...
- Molecular and biochemical characterization of a sand fly population from Sri Lanka: evidence for insecticide resistance due to altered esterases and insensitive acetylcholinesterase
S N Surendran
Department of Zoology, University of Jaffna, Jaffna, Sri Lanka
Bull Entomol Res 95:371-80. 2005
..There was preliminary evidence for elevated esterases and altered acetylcholinesterase in this population, the first report of these resistance mechanisms in sand ..
- Sex increases the efficacy of natural selection in experimental yeast populations
Matthew R Goddard
NERC Centre for Population Biology and Department of Biological Sciences, Imperial College London, Silwood Park Campus, Ascot SL5 7PY, UK
Nature 434:636-40. 2005
..We show that, as predicted by the theory, sex increases the rate of adaptation to a new harsh environment but has no measurable effect on fitness in a new benign environment where there is little selection...
- Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism
Molecular Biology Program, Memorial Sloan Kettering Cancer Center and Weill Graduate School of Medical Sciences of Cornell University, 1275 York Avenue, Box 97, New York, NY 10021, USA
Mol Cell 13:549-59. 2004
..Ski8 works with Spo11 to recruit other DSB proteins to meiotic chromosomes, implicating Ski8 as a scaffold protein mediating assembly of a multiprotein complex essential for DSB formation...
- Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY 10021, USA
Chromosoma 116:471-86. 2007
..These studies also suggest that an essential function of Rec102 and Rec104 is to connect Mei4 and Rec114 to Spo11...
- Differential gene expression of mammalian SPO11/TOP6A homologs during meiosis
Molecular and Structural Biology Division, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, CA 94550, USA
FEBS Lett 462:329-34. 1999
..2-13.3, a region that is amplified in some breast cancers. Sequence homology and differential expression together support a highly conserved role for SPO11 in the enzymatic cleavage of DNA that accompanies meiotic recombination...
- Methods to increase enantioselectivity of lipases and esterases
Uwe T Bornscheuer
Institute of Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstrasse 16, D 17487, Greifswald, Germany
Curr Opin Biotechnol 13:543-7. 2002
Lipases and esterases are frequently used in the synthesis of optically pure compounds; however, natural enzymes do not always show sufficiently high enantioselectivity...
- Paraoxonase (PON1) phenotype is a better predictor of vascular disease than is PON1(192) or PON1(55) genotype
G P Jarvik
Departments of Medicine, Division of Medical Genetics, University of Washington, Seattle, USA
Arterioscler Thromb Vasc Biol 20:2441-7. 2000
..These results support the benefit of a "level crossing" approach that includes intervening phenotypes in the study of complexly inherited disease...
- Rapid degradation of oseltamivir phosphate in clinical samples by plasma esterases
Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok 10400, Thailand
Antimicrob Agents Chemother 50:3197-9. 2006
The anti-influenza drug oseltamivir is an ester prodrug activated by hepatic carboxylesterases. Plasma esterases also convert up to 31...
- Denaturing action of urea and guanidine hydrochloride towards two thermophilic esterases
Pompea Del Vecchio
Department of Chemistry, University of Naples Federico II, Via Cintia, 45 80126 Naples, Italy
Biochem J 367:857-63. 2002
The stability of two thermophilic esterases, AFEST from Archaeoglobus fulgidus and EST2 from Alicyclobacillus acidocaldarius, against the denaturing action of urea and guanidine hydrochloride has been investigated by means of steady-..
- Roles played by esterase activity and by a sodium channel mutation involved in pyrethroid resistance in populations of Boophilus microplus (Acari: Ixodidae) collected from Yucatan, Mexico
J Med Entomol 42:1020-5. 2005
..It was not determined whether enhanced esterase-based hydrolytic activity was involved in pyrethroid resistance in the populations tested...
- Paraoxonase 192 Gln-->Arg polymorphism: an independent risk factor for nonfatal arterial ischemic stroke among young adults
Whitaker Cardiovascular Institute, Evans Department of Medicine, Boston University School of Medicine, Boston, Mass 02118, USA
Stroke 33:1459-64. 2002
..The association of these polymorphisms with the risk of AIS remains controversial...
- Current progress on esterases: from molecular structure to function
Biomedical Research Institute, Chiba University, Chiba, Japan
Drug Metab Dispos 30:488-93. 2002
..This article covers mechanisms of regulation of gene expression of the various esterases by developmental factors and xenobiotics, as well as the interplay between physiological and chemical regulation ..
- Inter-individual variability in esterases in human liver
Toxicology Unit, School of Clinical and Laboratory Sciences, 4th Floor, Devonshire Building, Devonshire Terrace, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4EA, UK
Biochem Pharmacol 74:932-9. 2007
..Lower correlations with larger alcohol leaving group parabens are consistent with more hCE2 involvement...
- Identification of residues in yeast Spo11p critical for meiotic DNA double-strand break formation
Robert L Diaz
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Mol Cell Biol 22:1106-15. 2002
..These results are consistent with a multimeric structure for Spo11p in vivo but may also indicate that the amount of Spo11 protein is not a limiting factor for DSB formation in normal cells...
- Effects of the synergists piperonyl butoxide and S,S,S-tributyl phosphorotrithioate on propoxur pharmacokinetics in Blattella germanica (Blattodea: Blattellidae)
, , Mexico
J Econ Entomol 94:1209-16. 2001
..The data demonstrate that unanticipated effects are possible with synergists and that caution must be exercised when interpreting synergist results...
- GDSL family of serine esterases/lipases
Casimir C Akoh
Department of Food Science and Technology, University of Georgia, Athens, GA 30602 7610, USA
Prog Lipid Res 43:534-52. 2004
GDSL esterases and lipases are hydrolytic enzymes with multifunctional properties such as broad substrate specificity and regiospecificity...
- Isolation and biochemical characterization of two novel metagenome-derived esterases
Molekulare Enzymtechnologie, , Lotharstrasse 1, 47057 Duisburg, Germany
Appl Environ Microbiol 72:3637-45. 2006
..Both esterases are approximately 380 amino acids in size and show similarity to beta-lactamases, indicating that they belong to ..
- Role of the N terminus in enzyme activity, stability and specificity in thermophilic esterases belonging to the HSL family
Institute of Protein Biochemistry, CNR, Via P. Castellino 111, 80131 Napoli, Italy
J Mol Biol 345:501-12. 2005
..This is the first report highlighting the importance of the "cap" domain in the HSL family, since the N terminus partly contributes to the building up of this structure...
- The effect of piperonyl butoxide on pyrethroid-resistance-associated esterases in Helicoverpa armigera (HÃ¼bner) (Lepidoptera: Noctuidae)
Susan J Young
Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK
Pest Manag Sci 61:397-401. 2005
..Biochemical studies have shown that pyrethroid-resistance-associated esterases in H armigera are inhibited by the insecticide synergist piperonyl butoxide (PBO)...
- Distinct DNA-damage-dependent and -independent responses drive the loss of oocytes in recombination-defective mouse mutants
Monica Di Giacomo
Molecular Biology Program, Memorial Sloan Kettering Cancer Center and Weill Graduate School of Medical Sciences of Cornell University, 1275 York Avenue, New York, NY 10021, USA
Proc Natl Acad Sci U S A 102:737-42. 2005
..The absence of ATM caused defects in folliculogenesis that were similar to those in Dmc1 mutants and that could be suppressed by Spo11 mutation, implying that oocyte death in Atm-deficient animals is a response to defective DSB repair...
- Esterase 2-oligodeoxynucleotide conjugates as sensitive reporter for electrochemical detection of nucleic acid hybridization
Laboratorium fur Biochemie, Universitat Bayreuth, Universitätsstr 30, 95440 Bayreuth, Germany
Biosens Bioelectron 22:1798-806. 2007
..Artificial single nucleotide mismatches in a 510-nucleotide ssDNA could be reliably determined using esterase 2-oligodeoxynucleotide conjugates as a reporter...
- Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity
Department of Biological Sciences, Northeast Structural Genomics Consortium, Columbia University, New York, NY 10027, USA
Proc Natl Acad Sci U S A 102:1773-8. 2005
..Our results suggest that SABP2 may be required to convert MeSA to SA as part of the signal transduction pathways that activate systemic acquired resistance and perhaps local defense responses as well...
- New thermophilic and thermostable esterase with sequence similarity to the hormone-sensitive lipase family, cloned from a metagenomic library
Department of Biotechnology, Yonsei University, 134 Sinchon-dong, Seodaemun-gu, Seoul 120-749, Korea
Appl Environ Microbiol 71:817-25. 2005
..An amino acid sequence comparison with other esterases and lipases revealed that the enzyme should be classified as a new member of the hormone-sensitive lipase family...
- Strategic selection of hyperthermophilic esterases for resolution of 2-arylpropionic esters
Amitabh C Sehgal
North Carolina State University, Department of Chemical Engineering, Raleigh, North Carolina 27695-7905, USA
Biotechnol Prog 19:1410-6. 2003
- Comparative acaricide susceptibility and detoxifying enzyme activities in field-collected resistant and susceptible strains of Tetranychus urticae
Thomas Van Leeuwen
Laboratory of Agrozoology, Department of Crop Protection, Faculty of Agricultural and Applied Biological Science, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
Pest Manag Sci 61:499-507. 2005
..Enhanced detoxification by increased activity of mono-oxygenases (MO) and esterases is at least partially responsible for the observed resistance and cross-resistance...
- Biochemical profiling in silico--predicting substrate specificities of large enzyme families
Institute of Technical Biochemistry, University of Stuttgart, Germany
J Biotechnol 124:108-16. 2006
..specificity of cholinesterases toward acetyl- and butyrylcholine, the changes of activity of lipases and esterases upon the size of the acid moieties, activity of lipases and esterases toward tertiary alcohols, and the ..
- MELDB: a database for microbial esterases and lipases
Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, KRIBB, P.O. Box 115, Yuseong, Daejeon 305-600, Republic of Korea
FEBS Lett 580:2736-40. 2006
MELDB is a comprehensive protein database of microbial esterases and lipases which are hydrolytic enzymes important in the modern industry...
- Detecting cellulase and esterase enzyme activities encoded by novel genes present in environmental DNA libraries
Helen C Rees
Department of Microbiology and Immunology, University of Leicester, University Road, Leicester, LE1 9HN, UK
Extremophiles 7:415-21. 2003
..Esterase/lipase-positive clones from Lake Elmenteita were isolated with a frequency of 1 in 30,000 from a library made from an olive-oil enrichment as compared to 1 in 100,000 from an environmental library...
- The role of esterases in the metabolism of ciclesonide to desisobutyryl-ciclesonide in human tissue
Toxicology Unit, Clinical and Laboratory Sciences, The University of Newcastle, Newcastle upon Tyne, UK
Biochem Pharmacol 73:1657-64. 2007
Ciclesonide (CIC) is an inhaled glucocorticosteroid. This study aimed to identify esterases involved in the metabolism of CIC to the active metabolite desisobutyryl-ciclesonide (des-CIC), and to measure hydrolysis rates in human liver, ..
- Chaperone-like activities of alpha-synuclein: alpha-synuclein assists enzyme activities of esterases
Division of Biotechnology and Molecular Engineering, College of Engineering, Ajou University, Suwon 443-749, Republic of Korea
Biochem Biophys Res Commun 346:1142-9. 2006
..which has no well-defined secondary or tertiary structure, can protect the enzyme activity of microbial esterases against stress conditions such as heat, pH, and organic solvents...
- Mechanism and control of meiotic recombination initiation
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, and Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
Curr Top Dev Biol 52:1-53. 2001
- Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11
Cell Biology Program Memorial Sloan Kettering Cancer Center New York, NY 10021, USA
Mol Cell 6:989-98. 2000
..Our results also support the view that mammalian checkpoint responses to meiotic recombination and/or synapsis defects are sexually dimorphic...
- Cloning and characterization of a periplasmic nuclease of Vibrio vulnificus and its role in preventing uptake of foreign DNA
S I Wu
Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, Republic of China
Appl Environ Microbiol 67:82-8. 2001
..We also demonstrated that Vvn was not required for the virulence of V. vulnificus mice...
- Evolutionary genetics of Drosophila esterases
J G Oakeshott
CSIRO Division of Entomology, Canberra ACT, Australia
Genetica 90:239-68. 1993
..This family shows minimal sequence similarities with other esterases but crystallographic data for a few non-drosophilid enzymes show that the family shares a distinctive overall ..
- The same amino acid substitution in orthologous esterases confers organophosphate resistance on the house fly and a blowfly
Division of Entomology, Commonwealth Scientific and Industrial Research Organisation, Canberra, Australia
Insect Biochem Mol Biol 29:675-86. 1999
..The data indicate identical catalytic mechanisms have evolved in orthologous Md alpha E7 and Lc alpha E7 molecules to endow diazinon-type resistance on the two species of higher Diptera...
- Biochemical detection of pyrethroid resistance mechanisms in Anopheles minimus in Thailand
Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
J Vector Ecol 28:108-16. 2003
..Expression of levels of non-specific esterases, monooxygenases, and glutathione S-transferases (GSTs) were measured...
- The mouse Spo11 gene is required for meiotic chromosome synapsis
P J Romanienko
Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health 20892, Bethesda, MD, USA
Mol Cell 6:975-87. 2000
..We speculate that there is an additional role for Spo11, after it generates DSBs, in synapsis...
- Human serum paraoxonase
University Department of Medicine, Manchester Royal Infirmary, United Kingdom
Gen Pharmacol 31:329-36. 1998
- Identification of a point mutation in an esterase gene in different populations of the southern cattle tick, Boophilus microplus
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station 77843 4467, USA
Insect Biochem Mol Biol 30:969-77. 2000
..Identification of this mutation and the strong signal detected in southern blot with this strain, suggest that esterases are contributing to detoxification of pyrethroid compounds, as a resistant mechanism in Mexican strains of the ..
- Screening, nucleotide sequence, and biochemical characterization of an esterase from Pseudomonas fluorescens with high activity towards lactones
Institute for Technical Biochemistry, University of Stuttgart, 70569 Stuttgart, Germany
Appl Environ Microbiol 65:477-82. 1999
..8 U/mg) to delta-valerolactone (36.5 U/mg). The activities towards the aliphatic esters were significantly lower; the only exception was the activity toward ethyl caprylate, which was the preferred substrate...
- An overview of the evolution of overproduced esterases in the mosquito Culex pipiens
Institut des Sciences de l Evolution, Laboratoire Génétique et Environnement C C 065, UMR CNRS 5554, Universite de Montpellier II, France
Philos Trans R Soc Lond B Biol Sci 353:1707-11. 1998
..One of these is the evolution of the overproduced esterases that are involved in resistance to organophosphate insecticides in the mosquito Culex pipiens...
- Paraoxonase active site required for protection against LDL oxidation involves its free sulfhydryl group and is different from that required for its arylesterase/paraoxonase activities: selective action of human paraoxonase allozymes Q and R
Lipid Research Laboratory, Technion Faculty of Medicine, The Rappaport Family Institute for Research in the Medical Sciences and Rambam Medical Center, Haifa, Israel
Arterioscler Thromb Vasc Biol 18:1617-24. 1998
- A family of serine esterases in lytic granules of cytolytic T lymphocytes
Cell 49:679-85. 1987
..CTLs) contain, in addition to the pore-forming protein perforin, a family of highly homologous serine esterases, granzymes A-H...
- Molecular analysis of evolutionary changes in the expression of Drosophila esterases
J P Brady
Department of Biology, Indiana University, Bloomington 47405
Proc Natl Acad Sci U S A 87:8217-21. 1990
..pseudoobscura, when isolated as a 4.5-kilobase restriction fragment, is not dosage compensated after integration into an autosome of D. melanogaster...
- Apolipoprotein J is associated with paraoxonase in human plasma
G J Kelso
Department of Pharmacology and Cell Biophysics, College of Medicine, University of Cincinnati, Ohio 45267
Biochemistry 33:832-9. 1994
..0 micrograms/mL. Both oxidation states of paraoxonase bind to apoJ with equal affinity. Our data combined with other evidence suggest that the plasma link of apoJ with paraoxonase will be implicated as a predictor of vascular damage...
- Quantification of intra- and extra-cellular thermophilic lipase/esterase production by Thermus sp
Department of Chemical Engineering, University of Vigo, 36200 Vigo, Spain
Biotechnol Lett 26:705-8. 2004
..All enzymes were stable at 80 degrees C over 30 min, and their activity towards fatty acid esters increased as substrate chain-length diminished (i.e. hydrolysis rate was up to 6-fold higher on p-nitrophenyl caproate than on laurate)...
- Molecular cloning, purification, and biochemical characterization of a novel pyrethroid-hydrolyzing esterase from Klebsiella sp. strain ZD112
Pei C Wu
State Key Laboratory of Biocontrol, School of Life Science, Zhongshan University, Guangzhou 510275, People's Republic of China
J Agric Food Chem 54:836-42. 2006
..were found by a database homology search using the nucleotide and deduced amino acid sequences of the esterases and lipases. EstP was heterologously expressed in E. coli and purified...
- Phospholipase action of platelet-activating factor acetylhydrolase, but not paraoxonase-1, on long fatty acyl chain phospholipid hydroperoxides
Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
J Biol Chem 282:100-8. 2007
- Human PON2 gene at 7q21.3: cloning, multiple mRNA forms, and missense polymorphisms in the coding sequence
Clinical Diabetes and Nutrition Section, Phoenix Epidemiology and Clinical Research Branch, National Institutes of Health, Phoenix, AZ 85016, USA
Gene 213:149-57. 1998
..We also describe two polymorphisms in the coding sequences that, in the protein deduced from the longest open reading frame, predict an alanine-to-glycine substitution at residue 147 and a serine-to-cysteine substitution at residue 310...
- Enzyme-like proteins from an unselected library of designed amino acid sequences
Department of Chemistry, Princeton University, Princeton, NJ 08544, USA
Protein Eng Des Sel 17:67-75. 2004
..The observed activity is similar to, or better than, that observed for several esterases designed previously using rational design or automated computational methods...
- Lactone-ring-cleaving enzyme: genetic analysis, novel RNA editing, and evolutionary implications
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake cho, Sakyo ku, Kyoto 606 8502, Japan
Proc Natl Acad Sci U S A 95:12787-92. 1998
..Lactonohydrolase was expressed under the control of the lac promoter in Escherichia coli...
- A novel esterase from Saccharomyces carlsbergensis, a possible function for the yeast TIP1 gene
M W Horsted
Department of Yeast Genetics, Carlsberg Laboratory, Copenhagen Valby, Denmark
Yeast 14:793-803. 1998
..cerevisiae TIP1 gene. The esterase preparation did not appear to contain significant amounts of other proteins than Tip1p, indicating that the TIP1 gene is the structural gene for the esterase...
- Identification of paraoxonase 3 in rat liver microsomes: purification and biochemical properties
Departamento de Medicina Legal y Toxicologia, Facultad de Medicina, Avda, Madrid 11, Granada 18071, Spain
Biochem J 376:261-8. 2003
..Some biochemical properties of PON3 were also studied, including optimum pH, K(m) and heat and pH stability...
- Dominant role of paraoxonases in inactivation of the Pseudomonas aeruginosa quorum-sensing signal N-(3-oxododecanoyl)-L-homoserine lactone
John F Teiber
The University of Texas Southwestern Medical Center, Department of Internal Medicine, Division of Epidemiology, 5323 Harry Hines Blvd, Dallas, TX 75390 8874, USA
Infect Immun 76:2512-9. 2008
..was previously shown to be hydrolytically inactivated by the paraoxonase (PON) family of calcium-dependent esterases, consisting of PON1, PON2, and PON3...
- DNA binding and cleavage by the periplasmic nuclease Vvn: a novel structure with a known active site
Chia Lung Li
Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China
EMBO J 22:4014-25. 2003
..This structural study suggests that Vvn hydrolyzes DNA by a general single-metal ion mechanism, and indicates how non-specific DNA-binding proteins may recognize DNA...
- The histidine 115-histidine 134 dyad mediates the lactonase activity of mammalian serum paraoxonases
Department of Biological Chemistry, Weizmann Institute of Science, Ullman Building, Rehovot 76100, Israel
J Biol Chem 281:7649-56. 2006
..To our knowledge, this is one of few examples of a histidine dyad in enzyme active sites and the first example of a hydrolytic enzyme with such a dyad...
- Differential expression of a cDNA clone in human liver versus hepatic cancer--highly homologous to aryl-dialkyl-phosphatase
K K Wang
National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute
Cell Res 7:79-90. 1997
..The biological significance of its differential expression in normal versus malignant tissue is discussed...
- Molecular cloning and expression pattern of rpr-1, a resiniferatoxin-binding, phosphotriesterase-related protein, expressed in rat kidney tubules
J A Davies
Department of Anatomy, University of Edinburgh Medical School, UK
FEBS Lett 410:378-82. 1997
..These results suggest an unsuspected link between the phosphotriesterase enzyme family and resiniferatoxin toxicity and pharmacology...
- Molecular cloning of enantioselective ester hydrolase from Bacillus pumilus DBRL-191
Biotechnology Division, Regional Research Laboratory, Canal Road, Jammu Tawi 180001, India
FEMS Microbiol Lett 249:113-20. 2005
..2kD and pI approximately 9.4. 3-D the structural model of the enzyme built by homology modelling using the atomic coordinates from the crystal structure of B. subtilis lipase (LipA) showed a compact minimal alpha/beta hydrolase fold...
- Genetic-dietary regulation of serum paraoxonase expression and its role in atherogenesis in a mouse model
D M Shih
Department of Medicine, School of Medicine, University of California Los Angeles, 90095, USA
J Clin Invest 97:1630-9. 1996
..In a set of recombinant inbred strains derived from the B6 and C3H parental strains, low paraoxonase mRNA levels segregated with aortic lesion development, supporting a role for paraoxonase in atherogenesis...
- Techniques to measure lipase and esterase activity in vitro
Department of Cell Biology, CIHR Group in Molecular and Cell Biology of Lipids, 328 HMRC, University of Alberta, Edmonton, Alta, Canada T6G 2S2
Methods 36:139-47. 2005
Lipases and esterases constitute a large category of enzymes. They are ubiquitous in nature, found in bacteria, fungi, and animals. The family members address a wide variety of structurally diverse substrates...
- A mouse kidney- and liver-expressed cDNA having homology with a prokaryotic parathion hydrolase (phosphotriesterase)-encoding gene: abnormal expression in injured and polycystic kidneys
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66160 7421, USA
Gene 168:157-63. 1996
..This cDNA represents the first eukaryotic sequence showing similarity to these prokaryotic genes. Based on this apparent homology, it has been named mpr56-1 (for mouse phosphotriesterase-related 56-1)...
- The human serum paraoxonase/arylesterase gene (PON1) is one member of a multigene family
S L Primo-Parmo
Department of Anesthesiology, University of Michigan Medical School, R5036 Kresge II Building, Ann Arbor, Michigan, 48109 0572, USA
Genomics 33:498-507. 1996
..The expanded number of PON genes will have important implications for future experiments designed to discover the individual functions, catalytic properties, and physiological roles of the paraoxonases...
- Human paraoxonases (PON1, PON2, and PON3) are lactonases with overlapping and distinct substrate specificities
Dragomir I Draganov
Department of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
J Lipid Res 46:1239-47. 2005
..None of the recombinant PONs protected low density lipoprotein against copper-induced oxidation in vitro...
- Pro-antibiotic substrates for the identification of enantioselective hydrolases
School of Chemical and Biological Engineering and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742, Korea
Biotechnol Lett 28:1181-5. 2006
A new growth-based screening method for the identification of enantioselective hydrolases, such as lipases and esterases, using pro-antibiotic substrates was devised...
- Killing of trypanosomes by the human haptoglobin-related protein
A B Smith
Department of Biochemistry and Molecular Genetics, School of Medicine and Dentistry, University of Alabama at Birmingham 35294, USA
Science 268:284-6. 1995
..An antibody to haptoglobin inhibited TLF activity. TLF was shown to exhibit peroxidase activity and to be inhibited by catalase. These results suggest that TLF kills trypanosomes by oxidative damage initiated by its peroxidase activity...
- Nucleotide sequence of a gene encoding an organophosphorus nerve agent degrading enzyme from Alteromonas haloplanktis
US Army Edgewood Research, Development, and Engineering Center, Aberdeen Proving Ground, MD 21010, USA
J Ind Microbiol Biotechnol 18:49-55. 1997
..These results corroborate the earlier conclusion that the OPAA is a type of X-Pro dipeptidase, and that X-Pro could be the native substrate for such an enzyme in Alteromonas cells...