esterases

Summary

Top Publications

  1. ncbi Mechanism and control of meiotic recombination initiation
    S Keeney
    Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, and Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Curr Top Dev Biol 52:1-53. 2001
  2. ncbi Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11
    F Baudat
    Cell Biology Program Memorial Sloan Kettering Cancer Center New York, NY 10021, USA
    Mol Cell 6:989-98. 2000
  3. ncbi SPO11 is required for sex-body formation, and Spo11 heterozygosity rescues the prophase arrest of Atm-/- spermatocytes
    Marina A Bellani
    Genetics and Biochemistry Branch, NIDDK, NIH, Bethesda, MD 20892, USA
    J Cell Sci 118:3233-45. 2005
  4. ncbi The effect of piperonyl butoxide on pyrethroid-resistance-associated esterases in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)
    Susan J Young
    Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK
    Pest Manag Sci 61:397-401. 2005
  5. ncbi Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination
    MONICA P COLAIACOVO
    Department of Developmental Biology, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Dev Cell 5:463-74. 2003
  6. ncbi Microbial carbohydrate esterases deacetylating plant polysaccharides
    Peter Biely
    Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia
    Biotechnol Adv 30:1575-88. 2012
  7. pmc A single amino acid substitution converts a carboxylesterase to an organophosphorus hydrolase and confers insecticide resistance on a blowfly
    R D Newcomb
    Commonwealth Scientific and Industrial Research Organisation, Division of Entomology, G P O Box 1700, Canberra, Australian Capital Territory 2601, Australia
    Proc Natl Acad Sci U S A 94:7464-8. 1997
  8. ncbi Identification of two novel esterases from a marine metagenomic library derived from South China Sea
    Xinmin Chu
    Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230027, China
    Appl Microbiol Biotechnol 80:615-25. 2008
  9. ncbi The same amino acid substitution in orthologous esterases confers organophosphate resistance on the house fly and a blowfly
    C Claudianos
    Division of Entomology, Commonwealth Scientific and Industrial Research Organisation, Canberra, Australia
    Insect Biochem Mol Biol 29:675-86. 1999
  10. ncbi Recombinational DNA double-strand breaks in mice precede synapsis
    S K Mahadevaiah
    Division of Developmental Genetics, National Institute for Medical Research, London, UK
    Nat Genet 27:271-6. 2001

Detail Information

Publications269 found, 100 shown here

  1. ncbi Mechanism and control of meiotic recombination initiation
    S Keeney
    Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, and Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Curr Top Dev Biol 52:1-53. 2001
    ....
  2. ncbi Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11
    F Baudat
    Cell Biology Program Memorial Sloan Kettering Cancer Center New York, NY 10021, USA
    Mol Cell 6:989-98. 2000
    ..Our results also support the view that mammalian checkpoint responses to meiotic recombination and/or synapsis defects are sexually dimorphic...
  3. ncbi SPO11 is required for sex-body formation, and Spo11 heterozygosity rescues the prophase arrest of Atm-/- spermatocytes
    Marina A Bellani
    Genetics and Biochemistry Branch, NIDDK, NIH, Bethesda, MD 20892, USA
    J Cell Sci 118:3233-45. 2005
    ....
  4. ncbi The effect of piperonyl butoxide on pyrethroid-resistance-associated esterases in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)
    Susan J Young
    Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK
    Pest Manag Sci 61:397-401. 2005
    ..Biochemical studies have shown that pyrethroid-resistance-associated esterases in H armigera are inhibited by the insecticide synergist piperonyl butoxide (PBO)...
  5. ncbi Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination
    MONICA P COLAIACOVO
    Department of Developmental Biology, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Dev Cell 5:463-74. 2003
    ..Our data also suggest that early prophase barriers to utilization of sister chromatids as repair templates do not depend on central region assembly...
  6. ncbi Microbial carbohydrate esterases deacetylating plant polysaccharides
    Peter Biely
    Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia
    Biotechnol Adv 30:1575-88. 2012
    ..The acetyl groups became targets of microbial carbohydrate esterases that evolved to overcome the complexity of the plant cell walls and that cooperate with glycoside hydrolases in ..
  7. pmc A single amino acid substitution converts a carboxylesterase to an organophosphorus hydrolase and confers insecticide resistance on a blowfly
    R D Newcomb
    Commonwealth Scientific and Industrial Research Organisation, Division of Entomology, G P O Box 1700, Canberra, Australian Capital Territory 2601, Australia
    Proc Natl Acad Sci U S A 94:7464-8. 1997
    ..Modeling of Asp137 in the homologous position in acetylcholinesterase suggests that Asp137 may act as a base to orientate a water molecule in the appropriate position for hydrolysis of the phosphorylated enzyme intermediate...
  8. ncbi Identification of two novel esterases from a marine metagenomic library derived from South China Sea
    Xinmin Chu
    Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230027, China
    Appl Microbiol Biotechnol 80:615-25. 2008
    ..were isolated from a marine microbial metagenomic library by functional screening, and the corresponding esterases EstA and EstB were biochemically characterized...
  9. ncbi The same amino acid substitution in orthologous esterases confers organophosphate resistance on the house fly and a blowfly
    C Claudianos
    Division of Entomology, Commonwealth Scientific and Industrial Research Organisation, Canberra, Australia
    Insect Biochem Mol Biol 29:675-86. 1999
    ..The data indicate identical catalytic mechanisms have evolved in orthologous Md alpha E7 and Lc alpha E7 molecules to endow diazinon-type resistance on the two species of higher Diptera...
  10. ncbi Recombinational DNA double-strand breaks in mice precede synapsis
    S K Mahadevaiah
    Division of Developmental Genetics, National Institute for Medical Research, London, UK
    Nat Genet 27:271-6. 2001
    ..Loss of gamma-H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'...
  11. ncbi A thermostable esterase from Thermoanaerobacter tengcongensis opening up a new family of bacterial lipolytic enzymes
    Lang Rao
    Chinese Academy of Sciences, Beijing, China
    Biochim Biophys Acta 1814:1695-702. 2011
    ..Based on the enzymatic properties and sequence alignment we concluded that the esterase EstA3 represented a novel bacterial lipolytic enzyme group and in chronological order this group was assigned as Family XIV...
  12. ncbi Identification and characterization of novel esterases from a deep-sea sediment metagenome
    Xiawei Jiang
    College of Life Sciences, Zhejiang University, Hangzhou, China
    Arch Microbiol 194:207-14. 2012
    ..The characterization of Est6 revealed that it was a cold-active esterase and exhibited the highest activity toward p-nitrophenyl butyrate (C4) at 20°C and pH 7.5...
  13. ncbi Overexpressed esterases in a fenvalerate resistant strain of the cotton bollworm, Helicoverpa armigera
    Shuwen Wu
    Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects Ministry of Agriculture, Nanjing 210095, China
    Insect Biochem Mol Biol 41:14-21. 2011
    ..with four putative carboxylesterase genes, all of which clustered within a phylogenetic clade of secreted midgut esterases. Quantitative PCR on these four genes showed several-fold greater expression in tissues of YGF compared to SCD ..
  14. pmc Self-interaction, nucleic acid binding, and nucleic acid chaperone activities are unexpectedly retained in the unique ORF1p of zebrafish LINE
    Mitsuhiro Nakamura
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori ku, Yokohama, Kanagawa, Japan
    Mol Cell Biol 32:458-69. 2012
    ..From this discovery, we propose that LINE ORF1ps play a general role in LINE integration by forming a complex with LINE RNA and rearranging its conformation...
  15. ncbi Distinction between esterases and lipases: a kinetic study with vinyl esters and TAG
    Henri Chahinian
    Laboratoire de Lipolyse Enzymatique, CNRS, Marseille, France
    Lipids 37:653-62. 2002
    ..carboxyl ester bonds (carboxyl ester hydrolases), we have compared the kinetic behavior of various lipases and esterases against solutions and emulsions of vinyl esters and TAG...
  16. ncbi Identification of genes encoding microbial glucuronoyl esterases
    Xin Liang Li
    National Center for Agricultural Utilization Research, USDA ARS, Peoria, IL 61604, USA
    FEBS Lett 581:4029-35. 2007
    ..jecorina. The product of the cip2 gene was purified to homogeneity and shown to exhibit glucuronoyl esterase activity...
  17. ncbi The esterase and PHD domains in CR1-like non-LTR retrotransposons
    Vladimir V Kapitonov
    Genetic Information Research Institute, Mountain View, California, USA
    Mol Biol Evol 20:38-46. 2003
    ..Unexpectedly, ES links together non-LTR retrotransposons and single-stranded RNA viruses like influenza C and coronaviruses, which are known to depend on their own ES...
  18. pmc Carbohydrate-active enzymes from the zygomycete fungus Rhizopus oryzae: a highly specialized approach to carbohydrate degradation depicted at genome level
    Evy Battaglia
    Microbiology and Kluyver Centre for Genomics of Industrial Fermentation, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    BMC Genomics 12:38. 2011
    ..Rhizopus oryzae is a zygomycete filamentous fungus, well-known as a saprobe ubiquitous in soil and as a pathogenic/spoilage fungus, causing Rhizopus rot and mucomycoses...
  19. ncbi Molecular and biochemical characterization of a sand fly population from Sri Lanka: evidence for insecticide resistance due to altered esterases and insensitive acetylcholinesterase
    S N Surendran
    Department of Zoology, University of Jaffna, Jaffna, Sri Lanka
    Bull Entomol Res 95:371-80. 2005
    ..There was preliminary evidence for elevated esterases and altered acetylcholinesterase in this population, the first report of these resistance mechanisms in sand ..
  20. pmc Synapsis-dependent and -independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegans
    Amy J MacQueen
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305 5329, USA
    Genes Dev 16:2428-42. 2002
    ....
  21. pmc Multiple biochemical and morphological factors underlie the production of methylketones in tomato trichomes
    Imri Ben-Israel
    Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel
    Plant Physiol 151:1952-64. 2009
    ..Furthermore, heterologous expression of MKS2 in Escherichia coli resulted in the production of methylketones in this host...
  22. pmc Endonucleolytic processing of covalent protein-linked DNA double-strand breaks
    Matthew J Neale
    Molecular Biology Programs, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 436:1053-7. 2005
    ..Our findings suggest a general mechanism for repair of protein-linked DSBs...
  23. pmc Multiple insecticide resistance mechanisms involving metabolic changes and insensitive target sites selected in anopheline vectors of malaria in Sri Lanka
    M Devika B Perera
    Department of Zoology, Faculty of Science, University of Peradeniya, Peradeniya, Sri Lanka
    Malar J 7:168. 2008
    ....
  24. ncbi cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases
    M Karlsson
    Section for Molecular Signalling, Department of Cell and Molecular Biology, Lund University, P O Box 94, S 221 00 Lund, Sweden
    J Biol Chem 272:27218-23. 1997
    ..Northern blot analysis revealed that monoglyceride lipase is ubiquitously expressed among tissues, with a transcript size of about 4 kilobases...
  25. pmc Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity
    Farhad Forouhar
    Department of Biological Sciences, Northeast Structural Genomics Consortium, Columbia University, New York, NY 10027, USA
    Proc Natl Acad Sci U S A 102:1773-8. 2005
    ..Our results suggest that SABP2 may be required to convert MeSA to SA as part of the signal transduction pathways that activate systemic acquired resistance and perhaps local defense responses as well...
  26. ncbi Pseudo-enzymatic hydrolysis of 4-nitrophenyl acetate by human serum albumin: pH-dependence of rates of individual steps
    Paolo Ascenzi
    Interdepartmental Laboratory of Electron Microscopy, University Roma Tre, Via della Vasca Navale 79, I 00146 Roma, Italy
    Biochem Biophys Res Commun 424:451-5. 2012
    ..0 ± 0.1 in the substrate-free HSA to 8.1 ± 0.1 in the HSA:NphOAc complex. Accordingly, diazepam inhibits competitively the HSA-catalyzed hydrolysis of NphOAc by binding to Tyr411...
  27. ncbi Current progress on esterases: from molecular structure to function
    Tetsuo Satoh
    Biomedical Research Institute, Chiba University, Chiba, Japan
    Drug Metab Dispos 30:488-93. 2002
    ..This article covers mechanisms of regulation of gene expression of the various esterases by developmental factors and xenobiotics, as well as the interplay between physiological and chemical regulation ..
  28. ncbi The classification of esterases: an important gene family involved in insecticide resistance--a review
    Isabela Reis Montella
    Laboratório de Fisiologia e Controle de Artrópodes Vetores, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brazil
    Mem Inst Oswaldo Cruz 107:437-49. 2012
    ..Historically, insecticide resistance has driven research on insect esterases and schemes for their classification...
  29. pmc Heterologous expression and biochemical characterisation of fourteen esterases from Helicoverpa armigera
    Mark G Teese
    CSIRO Ecosystem Sciences, Canberra, Australia
    PLoS ONE 8:e65951. 2013
    b>Esterases have recurrently been implicated in insecticide resistance in Helicoverpa armigera but little is known about the underlying molecular mechanisms...
  30. ncbi Cloning, recombinant expression and biochemical characterisation of novel esterases from Bacillus sp. associated with the marine sponge Aplysina aerophoba
    A Karpushova
    Institut für Technische Biochemie, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    Appl Microbiol Biotechnol 67:59-69. 2005
    Two novel esterases (EstB1 and EstB2) were isolated from a genomic library of Bacillus sp. associated with the marine sponge Aplysina aerophoba...
  31. ncbi Identification of human cytochrome P450 isoforms and esterases involved in the metabolism of mirabegron, a potent and selective β3-adrenoceptor agonist
    Shin Takusagawa
    Drug Metabolism Research Laboratories, Astellas Pharma Inc, Osaka, Japan
    Xenobiotica 42:957-67. 2012
    1. Human cytochrome P450 (CYP) enzymes and esterases involved in the metabolism of mirabegron, a potent and selective human β(3)-adrenoceptor agonist intended for the treatment of overactive bladder, were identified in in vitro studies...
  32. ncbi Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa
    Joseph Achaleke
    IRAD, PRASAC ARDESAC, Cotton Programme, Garoua, Cameroon
    Pest Manag Sci 65:1147-54. 2009
    ..Dose-response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance...
  33. ncbi Identification of a new subfamily of salt-tolerant esterases from a metagenomic library of tidal flat sediment
    Jeong Ho Jeon
    Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, P O Box 29, Ansan, 425 600, South Korea
    Appl Microbiol Biotechnol 93:623-31. 2012
    ..In this study, we demonstrated that the metagenomic approach using marine tidal flat sediment as a DNA source expanded the diversity of lipolytic enzyme-encoding genes...
  34. pmc Biochemical characterization and relative expression levels of multiple carbohydrate esterases of the xylanolytic rumen bacterium Prevotella ruminicola 23 grown on an ester-enriched substrate
    Mirjam A Kabel
    Royal Nedalco, Bergen op Zoom, The Netherlands
    Appl Environ Microbiol 77:5671-81. 2011
    We measured expression and used biochemical characterization of multiple carbohydrate esterases by the xylanolytic rumen bacterium Prevotella ruminicola 23 grown on an ester-enriched substrate to gain insight into the carbohydrate ..
  35. ncbi Catalytic promiscuity in the alpha/beta-hydrolase superfamily: hydroxamic acid formation, C--C bond formation, ester and thioester hydrolysis in the C--C hydrolase family
    Chen Li
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    Chembiochem 9:71-6. 2008
    ..family of alpha/beta-hydrolases contains enzymes of several different catalytic activities, including esterases, C--C hydrolases and cofactor-independent haloperoxidases (perhydrolases), but the molecular basis of this ..
  36. ncbi Isolation and characterization of cold-active family VIII esterases from an arctic soil metagenome
    Eun Young Yu
    Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, P O Box 107, Sinseongno 19, Yuseong, Daejeon 305 600, South Korea
    Appl Microbiol Biotechnol 90:573-81. 2011
    Functional screening for lipolytic enzymes at low temperatures resulted in the isolation of the novel cold-active esterases, EstM-N1 and EstM-N2, from a metagenomic DNA library of arctic soil samples...
  37. ncbi Screening and its potential application of lipolytic activity from a marine environment: characterization of a novel esterase from Yarrowia lipolytica CL180
    Jun Tae Kim
    Marine Biotechnology Center, Korea Ocean Research and Development Institute, Ansan, South Korea
    Appl Microbiol Biotechnol 74:820-8. 2007
    ..This study represents a novel esterase of type B1 carboxylesterase/lipase family from a marine isolate, showing a potential usage as a biocatalyst because of enantioselectivity toward racemic ofloxacin ester...
  38. ncbi Novel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment
    Yongfei Hu
    Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    FEMS Microbiol Ecol 72:228-37. 2010
    ..Furthermore, the whole sequence of fosmid pFL18 containing FLS18C and FLS18D was shotgun sequenced, and a total of 26 ORFs on it were analyzed and annotated...
  39. ncbi Growth-phase-dependent expression of the lipolytic system of Acinetobacter calcoaceticus BD413: cloning of a gene encoding one of the esterases
    R G Kok
    Department of Microbiology, E C Slater Institute, BioCentrum Amsterdam, The Netherlands
    J Gen Microbiol 139:2329-42. 1993
    ..0 kDa. The amino acid sequence of this protein shows homology to a number of lipolytic enzymes, most notably to esterases. Deletion of estA only partially abolished cell-bound esterase activity in A...
  40. ncbi The lipid droplet enzyme Tgl1p hydrolyzes both steryl esters and triglycerides in the yeast, Saccharomyces cerevisiae
    Anita Jandrositz
    Institute of Molecular Biosciences, SFB Biomembrane Research Center, University of Graz, Schubertstr 1, A8010 Graz, Austria
    Biochim Biophys Acta 1735:50-8. 2005
    ..cerevisiae. In a tgl1 deletion mutant, the mobilization of steryl esters in vivo was delayed, but not abolished, suggesting the existence of additional enzymes involved in steryl ester mobilization...
  41. pmc Identification of residues in yeast Spo11p critical for meiotic DNA double-strand break formation
    Robert L Diaz
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Mol Cell Biol 22:1106-15. 2002
    ..These results are consistent with a multimeric structure for Spo11p in vivo but may also indicate that the amount of Spo11 protein is not a limiting factor for DSB formation in normal cells...
  42. ncbi Enzymes from extremophiles
    D C Demirjian
    ThermoGen, Inc, Chicago, IL 60612, USA
    Curr Opin Chem Biol 5:144-51. 2001
    ..This has also led to a greater understanding of stability factors involved in adaptation of these enzymes to their unusual environments...
  43. ncbi C. elegans HIM-17 links chromatin modification and competence for initiation of meiotic recombination
    Kirthi C Reddy
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 118:439-52. 2004
    ..Furthermore, DSB levels and incidence of chiasmata can be modulated by loss of LIN-35/Rb. These and other data suggest that chromatin state governs the timing of DSB competence...
  44. ncbi Paraoxonase active site required for protection against LDL oxidation involves its free sulfhydryl group and is different from that required for its arylesterase/paraoxonase activities: selective action of human paraoxonase allozymes Q and R
    M Aviram
    Lipid Research Laboratory, Technion Faculty of Medicine, The Rappaport Family Institute for Research in the Medical Sciences and Rambam Medical Center, Haifa, Israel
    Arterioscler Thromb Vasc Biol 18:1617-24. 1998
    ....
  45. ncbi Plasma esterases and inflammation in ageing and frailty
    Ruth E Hubbard
    Department of Geriatric Medicine, School of Medicine, Cardiff University, 3rd Floor, Academic Centre, Llandough Hospital, Penarth, UK
    Eur J Clin Pharmacol 64:895-900. 2008
    b>Esterases are enzymes of drug metabolism known to be reduced in frail older people and during acute illness. The mechanism for this is unknown. The aim of this study was to examine esterase activity and inflammation in ageing and frailty.
  46. pmc Structural reorganization and preorganization in enzyme active sites: comparisons of experimental and theoretically ideal active site geometries in the multistep serine esterase reaction cycle
    Adam J T Smith
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    J Am Chem Soc 130:15361-73. 2008
    ..The results provide a useful guiding principle for de novo design of enzymes with multistep mechanisms...
  47. ncbi Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics
    Xianchun Li
    Department of Entomology and BIO5, University of Arizona, Tucson, Arizona 85721, USA
    Annu Rev Entomol 52:231-53. 2007
    ..e., cytochrome P450 monooxygenases (P450s), esterases, and glutathione-S-transferases (GSTs), are the focus of this review...
  48. pmc Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from antarctic desert soil
    Caroline Heath
    Department of Biotechnology, University of the Western Cape, Cape Town, South Africa
    Appl Environ Microbiol 75:4657-9. 2009
    ..The enzyme was active over a temperature range of 7 to 54 degrees C and at alkaline pH levels and is a potential candidate for industrial application...
  49. ncbi A survey of mutations in the Cochliomyia hominivorax (Diptera: Calliphoridae) esterase E3 gene associated with organophosphate resistance and the molecular identification of mutant alleles
    Renato Assis de Carvalho
    Laboratório de Genética Animal, Centro de Biologia Molecular e Engenharia Genética CBMEG, Universidade Estadual de Campinas UNICAMP, 13083 875 Campinas, SP, Brazil
    Vet Parasitol 140:344-51. 2006
    ..Further bioassays to determine the association of this mutation with OP resistance in C. hominivorax should allow the development of more effective strategies for managing this species...
  50. ncbi A family of serine esterases in lytic granules of cytolytic T lymphocytes
    D Masson
    Cell 49:679-85. 1987
    ..CTLs) contain, in addition to the pore-forming protein perforin, a family of highly homologous serine esterases, granzymes A-H...
  51. ncbi Sex increases the efficacy of natural selection in experimental yeast populations
    Matthew R Goddard
    NERC Centre for Population Biology and Department of Biological Sciences, Imperial College London, Silwood Park Campus, Ascot SL5 7PY, UK
    Nature 434:636-40. 2005
    ..We show that, as predicted by the theory, sex increases the rate of adaptation to a new harsh environment but has no measurable effect on fitness in a new benign environment where there is little selection...
  52. ncbi Ectopic expression of an esterase, which is a candidate for the unidentified plant cutinase, causes cuticular defects in Arabidopsis thaliana
    Kentaro Takahashi
    Department of Botany, Graduate School of Science, Kyoto University, Kyoto, Japan
    Plant Cell Physiol 51:123-31. 2010
    ..Our findings suggest that CDEF1 is a candidate gene for the unidentified plant cutinase...
  53. ncbi A new esterase EstD2 isolated from plant rhizosphere soil metagenome
    Myung Hwan Lee
    Department of Applied Biology, Dong A University, Busan, Republic of Korea
    Appl Microbiol Biotechnol 88:1125-34. 2010
    ..Our results suggest that the other hypothetical protein homologs of EstD2 might also be members of this novel family...
  54. ncbi GDSL family of serine esterases/lipases
    Casimir C Akoh
    Department of Food Science and Technology, University of Georgia, Athens, GA 30602 7610, USA
    Prog Lipid Res 43:534-52. 2004
    GDSL esterases and lipases are hydrolytic enzymes with multifunctional properties such as broad substrate specificity and regiospecificity...
  55. ncbi Cold-active esterase from Psychrobacter sp. Ant300: gene cloning, characterization, and the effects of Gly-->Pro substitution near the active site on its catalytic activity and stability
    Ljudmila Kulakova
    Laboratory of Microbial Biochemistry, Institute for Chemical Research, Kyoto University, Uji, Kyoto fu, Kyoto 611 0011, Japan
    Biochim Biophys Acta 1696:59-65. 2004
    ....
  56. ncbi Relationship between age and plasma esterases
    K Abou-Hatab
    Department of Geriatric Medicine, Academic Centre, Llandough Hospital, Penarth, UK
    Age Ageing 30:41-5. 2001
    ..RESULTS: we found no correlation between the activities of any of the enzymes measured and advancing age. CONCLUSION: age per se is not associated with reductions in the activities of esterase enzymes...
  57. ncbi Paraoxonase 192 Gln-->Arg polymorphism: an independent risk factor for nonfatal arterial ischemic stroke among young adults
    Barbara Voetsch
    Whitaker Cardiovascular Institute, Evans Department of Medicine, Boston University School of Medicine, Boston, Mass 02118, USA
    Stroke 33:1459-64. 2002
    ..The association of these polymorphisms with the risk of AIS remains controversial...
  58. ncbi High-resolution structure of ybfF from Escherichia coli K12: a unique substrate-binding crevice generated by domain arrangement
    Suk Youl Park
    Department of Chemistry and Institute of Basic Sciences, Chonnam National University, 300 Yongbong Dong, Buk Gu, Gwangju 500 757, Korea
    J Mol Biol 376:1426-37. 2008
    b>Esterases are one of the most common enzymes and are involved in diverse cellular functions...
  59. ncbi Functional expression and refolding of new alkaline esterase, EM2L8 from deep-sea sediment metagenome
    Hyo Jung Park
    Division of Biotechnology, The Catholic University of Korea, 43 1 Yokkok 2 dong, Wonmi gu, Bucheon, Gyeonggido 420 743, South Korea
    Protein Expr Purif 52:340-7. 2007
    ..Moreover, it was found to have an optimum pH of 10-11, thus revealing that it is an alkaline enzyme. In this paper, the new esterase EM2L8 buried in a deep-sea sediment became known on the surface and was characterized biochemically...
  60. ncbi Cloning, expression and characterization of a halotolerant esterase from a marine bacterium Pelagibacterium halotolerans B2T
    Xiawei Jiang
    College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, China
    Extremophiles 16:427-35. 2012
    ..With this halotolerance property, PE10 could be a candidate for industrial use...
  61. ncbi High-throughput assays for lipases and esterases
    Marlen Schmidt
    Institute of Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstr 16, D 17487 Greifswald, Germany
    Biomol Eng 22:51-6. 2005
    ..a considerable number of high-throughput screening (HTS) systems have been developed, especially for lipases and esterases. In this review, a range of HTS methods for the directed evolution of these hydrolases are covered...
  62. ncbi NTE and non-NTE esterases in brain membrane: kinetic characterization with organophosphates
    Iris Mangas
    Unidad de Toxicología, Instituto de Bioingenieria, Universidad Miguel Hernandez de Elche, Avda Universidad s n ES 03202, Elche, Alicante, Spain
    Toxicology 297:17-25. 2012
    ..inhibition") of two model compounds: paraoxon (non-neuropathy-inducer) and mipafox (neuropathy-inducer), with esterases of chicken brain membranes, an animal model, tissue and fractions, where neuropathy target esterase (NTE) was ..
  63. ncbi The Serratia marcescens bioH gene encodes an esterase
    Hiroyuki Akatsuka
    Discovery Research Laboratory, Tanabe Seiyaku Co, Ltd, Kawagishi 2 chome, Toda, 335 8505, Saitama, Japan
    Gene 302:185-92. 2003
    ..which belongs to the alpha/beta hydrolase-fold family comprising a wide variety of hydrolases including esterases. A catalytic triad composed of a nucleophilic residue (Ser80), an acidic residue (Asp206), and histidine (His234)..
  64. ncbi Multiple mutations and gene duplications conferring organophosphorus insecticide resistance have been selected at the Rop-1 locus of the sheep blowfly, Lucilia cuprina
    Richard D Newcomb
    Gene Technologies Sector, The Horticulture and Food Research Institute of New Zealand, Private Bag 92169 Auckland, New Zealand
    J Mol Evol 60:207-20. 2005
    ..Four new sequence haplotypes, not sampled in the survey of extant haplotypes, were obtained that are all associated with susceptibility. This is suggestive of a higher historical level of susceptible allelic diversity at this locus...
  65. pmc A new family of carbohydrate esterases is represented by a GDSL hydrolase/acetylxylan esterase from Geobacillus stearothermophilus
    Onit Alalouf
    Department of Biotechnology and Food Engineering, Technion Israel Institute of Technology, Haifa, Israel
    J Biol Chem 286:41993-2001. 2011
    Acetylxylan esterases hydrolyze the ester linkages of acetyl groups at positions 2 and/or 3 of the xylose moieties in xylan and play an important role in enhancing the accessibility of xylanases to the xylan backbone...
  66. pmc Cloning and biochemical characterization of a novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate)-hydrolyzing esterase from the newly isolated Nocardioides sp. strain SG-4G and its potential for use in enzymatic bioremediation
    Gunjan Pandey
    CSIRO Entomology, Clunies Ross St, Australian Capital Territory, Australia
    Appl Environ Microbiol 76:2940-5. 2010
    ..1 muM and 170 min(-1), respectively, for MBC. Radiation-killed, freeze-dried SG-4G cells showed strong and stable MBC detoxification activity suitable for use in enzymatic bioremediation applications...
  67. pmc Limited utility of acetoxymethyl (AM)-based intracellular delivery systems, in vivo: interference by extracellular esterases
    Paul D Jobsis
    Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, Bethesda, Maryland 20892, USA
    J Microsc 226:74-81. 2007
    ..We tested the hypothesis that extracellular esterases rapidly cleave the acetoxymethyl groups preventing the probes from entering cells, in vivo...
  68. ncbi Bivalve esterases as biomarker: identification and characterization in European cockles (Cerastoderma edule)
    Jeamylle Nilin
    Laboratory of Marine Ecotoxicology, Instituto de Ciências do Mar LABOMAR, Universidade Federal do Ceara, Fortaleza, Ceara, 60165 081, Brazil
    Bull Environ Contam Toxicol 88:707-11. 2012
    ..edule tissues using different substrates and specific inhibitors and identified the tissue distribution of esterases in this species...
  69. ncbi Histochemical determination of stereoselectivity of esterases in normal pancreas and pancreatic tubular adenocarcinoma of hamsters
    Y Yamazaki
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Biotech Histochem 73:23-31. 1998
    b>Esterases in normal hamster pancreas and pancreatic tubular adenocarcinoma of ductal origin induced by N-nitrosobis(2-oxopropyl)amine were stained in cryostat sections with mixtures of a diazonium salt (fast blue RR) and with each of the ..
  70. ncbi The role of esterases in the metabolism of ciclesonide to desisobutyryl-ciclesonide in human tissue
    Elaine Mutch
    Toxicology Unit, Clinical and Laboratory Sciences, The University of Newcastle, Newcastle upon Tyne, UK
    Biochem Pharmacol 73:1657-64. 2007
    Ciclesonide (CIC) is an inhaled glucocorticosteroid. This study aimed to identify esterases involved in the metabolism of CIC to the active metabolite desisobutyryl-ciclesonide (des-CIC), and to measure hydrolysis rates in human liver, ..
  71. ncbi Molecular cloning, purification, and biochemical characterization of a novel pyrethroid-hydrolyzing esterase from Klebsiella sp. strain ZD112
    Pei C Wu
    State Key Laboratory of Biocontrol, School of Life Science, Zhongshan University, Guangzhou 510275, People s Republic of China
    J Agric Food Chem 54:836-42. 2006
    ..were found by a database homology search using the nucleotide and deduced amino acid sequences of the esterases and lipases. EstP was heterologously expressed in E. coli and purified...
  72. pmc Characterization and heterologous gene expression of a novel esterase from Lactobacillus casei CL96
    Young J Choi
    Department of Food Science and Agricultural Chemistry, McGill University, Ste Anne de Bellevue, Quebec, H9X 3V9, Canada
    Appl Environ Microbiol 70:3213-21. 2004
    ..0 and 37 degrees C, respectively. Among the pNP (p-nitrophenyl) esters tested, the most selective substrate was pNP-caprylate (C(8)), with K(m) and k(cat) values of 14 +/- 1.08 microM and 1,245 +/- 42.3 S(-1), respectively...
  73. ncbi Mutational analysis of N-linked glycosylation of esterase 6 in Drosophila melanogaster
    M A Myers
    CSIRO Division of Entomology, Canberra, Australia
    Biochem Genet 34:201-18. 1996
    ..The latter effect may compromise the role of the transferred enzyme in stimulating egg-laying and delaying receptivity to remating...
  74. ncbi Lipases or esterases: does it really matter? Toward a new bio-physico-chemical classification
    Yassine Ben Ali
    Laboratoire de Biochimie et de Genie Enzymatique des Lipases, ENIS, University of Sfax, Sfax, Tunisia
    Methods Mol Biol 861:31-51. 2012
    Carboxylester hydrolases, commonly named esterases, consist of a large spectrum of enzymes defined by their ability to catalyze the hydrolysis of carboxylic ester bonds and are widely distributed among animals, plants, and microorganisms...
  75. pmc The active site of a carbohydrate esterase displays divergent catalytic and noncatalytic binding functions
    Cedric Montanier
    Institute for Cell and Molecular Biosciences, Newcastle University, The Medical School, Newcastle upon Tyne, UK
    PLoS Biol 7:e71. 2009
    ..exhibits a noncatalytic cellulose-binding function but also shares sequence identity with the CE2 family of esterases. Here we show that, unlike other CE2 members, the CtCE2 domain displays divergent catalytic esterase and ..
  76. ncbi Improving the ex vivo stability of drug ester compounds in rat and dog serum: inhibition of the specific esterases and implications on their identity
    Matthias Koitka
    INSERM U850, Universite de Limoges, Faculte de Medecine, 2 rue du Dr Marcland, 87025 Limoges, France
    J Pharm Biomed Anal 51:664-78. 2010
    ..Furthermore, we found indications of the identity of the drug-specific esterases by means of their inhibitor sensitivity as well as by protein purification and identification...
  77. ncbi R192Q paraoxonase gene variant is associated with a change in HDL-cholesterol level during dietary caloric restriction in nondiabetic healthy males
    Tatsuo Obata
    First Department of Internal Medicine, National Defense Medical College, Saitama, Japan
    J Atheroscler Thromb 10:57-62. 2003
    ..PON R192Q polymorphism could affect HDL-C levels after caloric restriction presumably due to decreased HL activity and altered insulin resistance...
  78. ncbi The role of paraoxonase (PON1) in the detoxication of organophosphates and its human polymorphism
    L G Costa
    Department of Environmental Health, University of Washington, Seattle 98105, USA
    Chem Biol Interact 119:429-38. 1999
    ..These observations underline the importance of considering both genetic variability of enzyme isoform as well as enzyme level (PON1 status) and the developmental time course of appearance of PON1 in developing risk assessment models...
  79. ncbi Single residues dictate the co-evolution of dual esterases: MCP hydrolases from the α/β hydrolase family
    María Alcaide
    Spanish National Research Council CSIC, Institute of Catalysis, 28049 Madrid, Spain
    Biochem J 454:157-66. 2013
    ..The results may open up new research avenues into comparative catalytic models, structural and mechanistic studies, and biotechnological applications of MCP hydrolases. ..
  80. ncbi A thermostable feruloyl-esterase from the hemicellulolytic bacterium Thermobacillus xylanilyticus releases phenolic acids from non-pretreated plant cell walls
    Harivony Rakotoarivonina
    UMR614 Fractionnement des AgroRessources et Environnement, INRA, 51686 Reims, France
    Appl Microbiol Biotechnol 90:541-52. 2011
    ..4 kDa, similar to feruloyl-esterases from cellulolytic bacteria and fungi...
  81. ncbi Characterization of digestive gland esterase-lipase activity of juvenile redclaw crayfish Cherax quadricarinatus
    Silverio López-López
    Laboratorio de Fisiología y Bioquímica Comparada, Centro de Investigaciones Biologicas del Noroeste, Mar Bermejo 195, Col Playa Palo Santa Rita, La Paz, BCS 23090, Mexico
    Comp Biochem Physiol B Biochem Mol Biol 135:337-47. 2003
    ..The enzyme activity diminished as the length of the fatty acid chain of substrates increased. Molecular masses for four isozymes were 43, 46, 63 and 118 kDa, respectively, as determined by PAGE...
  82. ncbi Purification and cloning of an esterase from the weed black-grass (Alopecurus myosuroides), which bioactivates aryloxyphenoxypropionate herbicides
    Ian Cummins
    School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, UK
    Plant J 39:894-904. 2004
    ....
  83. ncbi Functions of the siderophore esterases IroD and IroE in iron-salmochelin utilization
    Mingang Zhu
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Microbiology 151:2363-72. 2005
    ..It is suggested that homologues in other bacteria are also periplasmic IroE-type esterases of siderophores.
  84. ncbi Isolation and characterization of the early nodule-specific protein homologue (Hev b 13), an allergenic lipolytic esterase from Hevea brasiliensis latex
    Siti Arija M Arif
    Rubber Research Institute of Malaysia, 50450 Kuala Lumpur, Malaysia
    J Biol Chem 279:23933-41. 2004
    ..The purified protein showed lipase and esterase activities and may be involved in plant defense...
  85. ncbi Esterases from Bacillus subtilis and B. stearothermophilus share high sequence homology but differ substantially in their properties
    E Henke
    Institute of Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstrasse 16, 17487 Greifswald, Germany
    Appl Microbiol Biotechnol 60:320-6. 2002
    ..Despite the high homology, both esterases revealed remarkable differences in their properties...
  86. ncbi Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system
    Martin Ruppert
    Department of Pharmaceutical Biology, Institute of Pharmacy, Johannes Gutenberg University, Staudinger Weg 5, 55128, Mainz, Germany
    Planta 222:888-98. 2005
    ..This expression allowed molecular analysis of AAE for the first time and increased the number of functionally expressed alkaloid genes from Rauvolfia now to eight, and the number of ajmaline pathway-specific cDNAs to a total of six...
  87. ncbi Purification of ENOD8 proteins from Medicago sativa root nodules and their characterization as esterases
    David Pringle
    Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104, USA
    Plant Physiol Biochem 42:73-9. 2004
    ..Considering ENOD8 protein sequence similarity to an exopolygalacturonase/EP4/iEP4 and lanatoside 15'-O-acetylesterase with the results presented here predicts that ENOD8 substrates could be acetylated oligo- or polysaccharides...
  88. ncbi Effects of four host plants on susceptibility of Spodoptera litura (Lepidoptera: Noctuidae) larvae to five insecticides and activities of detoxification esterases
    Ming Xue
    Department of Entomology, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, China
    Pest Manag Sci 66:1273-9. 2010
    ..The susceptibility of S. litura larvae reared on tobacco, Chinese cabbage, cowpea and sweet potato to phoxim, chlorfenapyr, methomyl, fenvalerate and emamectin benzoate under laboratory conditions was determined...
  89. ncbi Characterization and its potential application of two esterases derived from the arctic sediment metagenome
    Jeong Ho Jeon
    Marine Biotechnology Research Centre, Korean Ocean Research and Development Institute, Ansan, South Korea
    Mar Biotechnol (NY) 11:307-16. 2009
    ..The esterases genes were overexpressed in Escherichia coli by co-expressing GroEL-GroES chaperonine, and the recombinant ..
  90. ncbi Two glucuronoyl esterases of Phanerochaete chrysosporium
    Miroslava Duranová
    Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dubravska cesta 9, 845 38, Bratislava, Slovakia
    Arch Microbiol 191:133-40. 2009
    ..Comparison of the amino acid sequences of identified and putative glucuronoyl esterases to other types of carbohydrate esterases (CE) confirmed that they form a separate family of CEs...
  91. ncbi A novel esterase from Bacillus subtilis (RRL 1789): purification and characterization of the enzyme
    Peerzada Kaiser
    Regional Research Laboratory, Canal Road, Jammu Tawi 180001, India
    Protein Expr Purif 45:262-8. 2006
    ..The N-terminal amino acid sequence of BSE determined, as Met-Thr-Pro-Glu-Iso-Val-Thr-Thr-Glu-Tyr-Gly- revealed similarity with the N-terminal amino acid sequence of p-nitrobenzylesterase of B. subtilis...
  92. ncbi Characterization of two metagenome-derived esterases that reactivate chloramphenicol by counteracting chloramphenicol acetyltransferase
    Weixin Tao
    Department of Medical Bioscience, Dong A University, Busan 604 714, Korea
    J Microbiol Biotechnol 21:1203-10. 2011
    ..we investigated lipolytic enzymes selected from an alluvial soil metagenomic library, and identified two novel esterases, EstDL26 and EstDL136...
  93. ncbi Indication for a new lipolytic enzyme family: isolation and characterization of two esterases from a metagenomic library
    Sally Bayer
    Institute of Biochemistry, University of Leipzig, Germany
    J Mol Microbiol Biotechnol 18:181-7. 2010
    ..EstGK1 and EstZ3 have only minor overall sequence similarity to known esterases. We propose that, together with other hypothetical enzymes, they constitute a new family of lipolytic enzymes...
  94. ncbi Functional proteomic analysis of lipases and esterases in cultured human adipocytes
    Maximilian Schicher
    Institute of Biochemistry, Graz University of Technology, Graz, Austria
    J Proteome Res 9:6334-44. 2010
    ..Both of them showed pronounced hydrolytic activities on hydrophobic acylglycerols and therefore may be considered lipases. The physiological functions of the novel lipolytic proteins in vivo are currently subject to investigation...
  95. ncbi Gene cloning and characterization of a novel thermophilic esterase from Fervidobacterium nodosum Rt17-B1
    Shanshan Yu
    Jilin University, Changchun, China
    Acta Biochim Biophys Sin (Shanghai) 42:288-95. 2010
    ..high with a half-life of 80 min at 70 degrees C, but less stable compared with some other hyperthermophilic esterases. A structural model was constructed using acetylesterase from Aspergillus aculeatus as a template...
  96. ncbi Characterization of novel esterases in insecticide-resistant mosquitoes
    Feng Cui
    State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Insect Biochem Mol Biol 37:1131-7. 2007
    ..detected Ester alleles, Ester(B10) and Ester(11) (including associated Ester(A11) and Ester(B11)), coding for esterases B10 and A11-B11, respectively, are characterized qualitatively and quantitatively...
  97. ncbi Production and characterization of two N-terminal truncated esterases from Thermus thermophilus HB27 in a mesophilic yeast: effect of N-terminus in thermal activity and stability
    Pablo Fuciños
    Department of Analytical and Food Chemistry, University of Vigo, Campus of Ourense, As Lagoas, 32004 Ourense, Spain
    Protein Expr Purif 78:120-30. 2011
    ..Minor differences were observed in the substrate specificity. Our results suggest that the N-terminal segment is critical for maintaining the hyperthermophilic function and stability...
  98. ncbi Biochemical and physiological studies of soluble esterases from Drosophila melanogaster
    M J Healy
    CSIRO Division of Entomology, Canberra, ACT, Australia
    Biochem Genet 29:365-88. 1991
    Twenty-two soluble esterases have been identified in D. melanogaster by combining the techniques of native polyacrylamide gel electrophoresis and isoelectric focusing...
  99. ncbi MRI cerebral white matter lesions and paraoxonase PON1 polymorphisms : three-year follow-up of the austrian stroke prevention study
    R Schmidt
    Department of Neurology, Karl Franzens University, Graz, Austria
    Arterioscler Thromb Vasc Biol 20:1811-6. 2000
    ..05/mm Hg), and LL paraoxonase genotype (odds ratio, 2. 65) to be significant predictors of WML progression. These data suggest that the LL PON1 genotype at position 54 influences the extent and progression of WMLs in elderly subjects...
  100. ncbi Identification and biochemical characterization of a GDSL-motif carboxylester hydrolase from Carica papaya latex
    Slim Abdelkafi
    CNRS, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse, Marseille, France
    Biochim Biophys Acta 1791:1048-56. 2009
    ....
  101. ncbi The SGNH-hydrolase of Streptomyces coelicolor has (aryl)esterase and a true lipase activity
    Ana Bielen
    Laboratory of Molecular Genetics, Division of Molecular Biology, Ruder Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia
    Biochimie 91:390-400. 2009
    ..coelicolor SGNH-hydrolase generated three distinct groups consisting of proteins from Actinomycetales, Ascomycota and Nematoda. At present it seems that these enzymes are most conserved among soil inhabiting organisms...

Research Grants63

  1. A Soft Topical Antiandrogenic Drug
    Wei Chu Xu; Fiscal Year: 2013
    ..on the market or in development, (S)-HYG-440 was designed to avoid systemic activity by taking advantage of esterases and hydrolases found in most tissues in the body for metabolic deactivation, i.e. as a metabolically "soft" drug...
  2. Novel mechanisms by which aspirin might protect against atherosclerosis
    Sampath Parthasarathy; Fiscal Year: 2012
    ..Hydrolysis was competed for by substrates of "aryl esterases" that prompted us to propose that paraoxonase 1 (PON 1) type of enzymes might be involved in the metabolism of ..
  3. Development Of An Eye Drop To Treat Presbyopia
    William H Garner; Fiscal Year: 2012
    ..The co-drug elicits no acute toxicology in Draize testing in rabbits. The co-drug is cleaved by cellular esterases to release a pro-reductant, that when converted to active reducing agent by cellular oxidoreductases, increases ..
  4. Tissue Kallikrein In Immune Mediated Nephritis
    Quan Zhen Li; Fiscal Year: 2011
    ..Klks constitute a multi-gene family of serine esterases with a wide spectrum of biological functions...
  5. Role of Sialic Acid Modification in ALL Survival and Drug Resistance
    NORA C HEISTERKAMP; Fiscal Year: 2013
    ..during death due to 9-O-acetylation removal from Sias in pre-B ALL cells, by expression of inducible 9-O-acetyl esterases in human pre-B ALL and mouse wild type and GD3-/- pre-B ALL cells in co-culture with protective stroma...
  6. Dietary Rice Bran Phytochemicals and Microbial Metabolites for Colon Chemoprevent
    Tiffany L Weir; Fiscal Year: 2013
    ..such as gamma-oryzanol to its bioactive metabolite ferulic acid via the activity of microbial ferulate esterases. The proposed research project will identify the change in microbes and metabolites found in stool using 454 ..
  7. Immunopharmacotherapy for the Treatment of Cocaine Abuse
    Kim D Janda; Fiscal Year: 2013
    ..Specifically, the poor catalytic efficiency of cocaine-degrading mAbs, the uncertain druggability of cocaine esterases, and the weak immunogenicity of current active vaccines, which represent the only anti-cocaine therapy tested in ..
  8. Relationship of Blood Esterases, Pesticide Exposure and Cardiovascular Disease
    Janice Chambers; Fiscal Year: 2009
    ..In addition, we anticipate that we will develop more simplified assay methods for PON1 and CaE that will be more amenable for use as a clinical biomarker. ..
  9. Molecular Mechanisms of Dietary Fat Digestion by Pancreatic Lipases
    Mark E Lowe; Fiscal Year: 2012
    ..Lipases differ from esterases that act on water soluble substrates in that lipases must absorb to the interface of the substrate emulsion ..
  10. High-activity mutants of cocaine esterase for treatment of drug addiction
    Chang Guo Zhan; Fiscal Year: 2012
    Enhancing cocaine metabolism by administration of cocaine esterases has been recognized as a promising treatment strategy for cocaine overdose and addiction...
  11. Mechanism and Anti-Cancer Activity of SCFA-Hexosamine Analogs
    Kevin J Yarema; Fiscal Year: 2013
    ..through the sialic acid biosynthetic pathway due to ManNAc generated after complete removal of n-butyrate by esterases. During the initial funding period of this project, it was discovered that partial hydrolysis products of ..
  12. MOLECULAR BASIS OF CANNABINOID ACTIVITY
    Alexandros Makriyannis; Fiscal Year: 2013
    ..to extend our goals by new findings on the effects of enzymatic endocannabinoid deactivation by specific esterases. We now propose to study the three key brain esterases involved in endocannabinoid deactivation, namely, ..
  13. Effects of Biofilm Activities and Dental Resin Composite Compositions on the Deve
    Clifton M Carey; Fiscal Year: 2013
    ..margins include: interfacial stresses from contractions induced by polymerization shrinkage of resins, salivary esterases, acidogenic microbe-generated biofilms, and deformations from cyclic loading and oral thermal fluctuations...
  14. MOLECULAR STRUCTURE OF STEROIDS
    William Duax; Fiscal Year: 2001
    ..A comparison of the structure of Cc ChE with those of other esterases should reveal the basis for cholesterol specificity and permit design of specific inhibitors of ChE that would ..
  15. HYDROLYTIC ENZYMES IN THE METABOLISM OF TOXINS
    Bruce Hammock; Fiscal Year: 2007
    ..This proposal targets two enzyme classes, epoxide hydrolases (EHs) and esterases, which are both members of the alpha/beta-hydrolase fold family...
  16. Control of Urban and Peri-urban Culex Mosquitoes
    Gregory Lanzaro; Fiscal Year: 2007
    ..this by developing novel pyrethoid-like fluorescent substrates for detection of P450 mixed function oxidases and esterases in Culex mosquitoes. These new substrates are highly sensitive...
  17. STEROID METABOLISM AND ACTION IN CANCER
    Mortimer Levitz; Fiscal Year: 1993
    ..the proposal would exploit the MCF-7 breast cancer cell line as a model to test the role of estrogen- sensitive esterases in the control of cell proliferation...
  18. MEIBOMIAN KERATOCONJUNCTIVITIS
    James McCulley; Fiscal Year: 1999
    ..In addition, isolated bacteria have been tested for production of specific exoenzymes such as lipases and esterases which could produce secondary lipid changes...
  19. Metabolism and Age-Related Organophosphate Toxicity
    Janice Chambers; Fiscal Year: 2005
    ..levels in both adults and juveniles are strongly related to the levels and the efficiency of several protective esterases. We plan to test our hypothesis by investigating the following two specific aims: 1...
  20. Perfluorocarbon Materials As Drug Delivery Vehicles
    Hans Joachim Lehmler; Fiscal Year: 2004
    ..These prodrugs show a high solubility in PFOB and are also water soluble, readily hydrolyzed in the presence of esterases and show little cytotoxicity...
  21. BLOOD-BRAIN BARRIER TRANSPORT OF THYMIDINE ANALOGS
    Ashim Mitra; Fiscal Year: 1990
    ..5'-ester derivatives have been selected for their wide range of lipophilicities and structural specificities for esterases. The latentiated analogues will undergo appreciable transport (compared to IDU itself) across the blood-brain ..
  22. GROWTH AND NUTRITION IN ALAGILLE SYNDROME
    ANDREW MULBERG; Fiscal Year: 1999
    ..on not only the solubilization by bile acids into mixed micelles, but also requires pancreatic and intestinal esterases for hydrolysis before traversing the aqueous water layer in the intestinal lumen into the enterocyte...
  23. ORAL MUCOSAL CELL RESPONSES TO DENTAL RESIN COMPONENTS
    George Schuster; Fiscal Year: 1999
    ..The ability of cells and tissue esterases to hydrolyze the materials will also be assessed using cell homogenates and commercial tissue esterases...
  24. Improving Absorption and Targeting of Antiviral Drugs
    John Hilfinger; Fiscal Year: 2009
    ..designing prodrugs targeted to transporters expressed in human intestine and able to be activated by endogenous esterases or amidases that release the absorbed drug in vivo from its prodrug form...
  25. Synthesis of New NNRTLs for the Treatment of AIDS
    Mark Cushman; Fiscal Year: 2005
    ..of the ADAMs is compromised by the presence of three methyl ester moieties that are readily hydrolyzed by plasma esterases. Consequently, the main goal of the present project is to find suitable replacements for these three labile ..
  26. CALCIUM INDEPENDENT PLA2 AND ISCHEMIC CELL INJURY
    Didier Portilla; Fiscal Year: 2000
    ..which agrees with the consensus sequence surrounding active site serines, G-X-S-X-U, observed in many carboxyl esterases including lipases...
  27. WATER-SOLUBLE PRODRUGS OF TAXOL AGAINST COLON CANCER
    LUKE LAM; Fiscal Year: 1993
    ..salts have problems of stability, toxicity, physiological pH disturbance and non-specific hydrolysis by esterases. Although there are more than 60 derivatives known, taxol beta-glucosides and beta-galactosides are not known...
  28. SELECTIVE DEPLETION OF ALKYLTRANSFERASE IN TUMOR CELLS
    M Dolan; Fiscal Year: 1999
    ..less potent against the pure AGT protein than their deesterified counterpart requiring hydrolysis by cellular esterases for a highly potent agent...
  29. BIODEGRADABLE PESTICIDES BASED ON HORMONE METABOLISM
    Bruce Hammock; Fiscal Year: 1980
    ..This aim will be accomplished by studying JH esterases and epoxide hydrases. The mechanism of JH esterase regulation will be determined...
  30. Role of fibrate mechanism in diabetes and its complications
    GANESARATNAM BALENDIRAN; Fiscal Year: 2010
    ..Following oral administration, fenofibrate is rapidly hydrolyzed by esterases to its active metabolite, fenofibric acid...
  31. RESEARCH CAREER DEVELOPMENT IN BIOCHEMICAL TOXICOLOGY
    Janice Chambers; Fiscal Year: 1993
    ..of the activated metabolites by brain (the target tissue) and liver, along with the potential mechanisms (esterases and other binding proteins) present in liver and blood which would protect the target AChE...
  32. SERINE ESTERASES IN HUMAN CYTOLYTIC LYMPHOCYTES
    Bo Dupont; Fiscal Year: 1991
    The objective of this proposal is to isolate and characterize genes encoding serine esterases (SEs) from human natural killer (NK) and cytotoxic T-lymphocytes (CTL) in order to investigate the role of these molecules in cellular ..
  33. SELECTIVE THERAPY OF TUMORS THAT OVEREXPRESS C MYC
    PHILIP POTTER; Fiscal Year: 2006
    The anticancer prodrug CPT-11 is activated by esterases to yield the potent topoisomerase I inhibitor SN-3S...
  34. Development of Esterases for the Treatment of Cocaine Overdose and Abuse
    James Woods; Fiscal Year: 2009
    ..These procedures are designed to prolong the enzyme's duration of action with the hopeful outcome that some insight will be obtained into how to make this substance useful in the long-term treatment of cocaine abuse. ..
  35. IN VIVO CHEMILUMINESCENT ACTIVATION OF PHOTOSENSITIZERS
    Susan Carpenter; Fiscal Year: 1999
    ..The second type of tethered molecules will be those which maintain their integrity in the presenc of esterases. This design will take advantage of a very efficient intramolecular energy transfer between hypericin and the ..
  36. ISOLATION AND STRUCTURE OF MICROSOMAL MEMBRANE PROTEINS
    Juris Ozols; Fiscal Year: 2002
    ..of premature proteolysis of altered gene products, atherosclerosis (steroyl CoA desaturase, and drug metabolism (esterases and FMO's))...
  37. TOPS-Ester: Pharmacokinetics and Imaging in Human Skin
    CARLETON HSIA; Fiscal Year: 2003
    ..cells of the epidermis and dermis, the succinate-ester moiety of the molecule is hydrolyzed by intracellular esterases to succinate anions; thereby facilitating intracellular retention of negatively charged carboxylate derivatives ..
  38. Cholinergic Signaling in the Brain
    Sukumar Vijayaraghavan; Fiscal Year: 2006
    ..The effects of acetylcholine esterases on nAChR responses will be examined in order to characterize transmitter diffusion and its ability to act on non-..
  39. ROLES OF CHOLESTERYL ESTER HYDROLASES IN THE LIVER
    William Grogan; Fiscal Year: 1999
    ..and promoter characteristics will be compared with other enzymes of cholesterol metabolism and other carboxyl esterases. The third aim is to measure cholesterol ester hydrolase protein and mRNA in various regions of the liver as ..
  40. COCAINE, HEROIN & OPIOID METABOLISM BY CARBOXYLESTERASES
    WILLIAM BOSRON; Fiscal Year: 2000
    ..These major metabolites are hot pharmacologically active as psychomotor stimulants. Two different esterases have been identified in liver that catalyze the hydrolysis of cocaine to benzoylecgonine and ecgonine methyl ..
  41. INHIBITION OF DNA REPAIR TO ENHANCE CHEMOTHERAPY
    ANTHONY PEGG; Fiscal Year: 2000
    ..8-substituted BG derivatives; compounds with ester linkages that are activated as AGT inhibitors by esterases; and benzyl pyrimidine derivatives which are very potent but rapidly cleared...
  42. MOLECULAR EVOLUTION OF B-ESTERASES IN DROSOPHILA
    ROLLIN RICHMOND; Fiscal Year: 1990
    ..These data will be used to assess the adaptive significance of allozyme variation at the esterase-6 locus and to construct hypotheses about evolutionary changes in sequences which regulate the expression of structural genes...
  43. FASEB SUMMER CONFERECE ON PHOSPHOLIPASES
    EDWARD DENNIS; Fiscal Year: 1992
    ..There is no other conference dedicated to these ubiquitous and important esterases. All aspects of their chemistry and their roles in diseased states will be covered in invited talks, discussion ..
  44. DEGRANULATION FUNCTIONS OF CYTOTOXIC T LYMPHOCYTES
    William Clark; Fiscal Year: 1992
    ..While it is certain that a number of different serine esterases reside in granules, little else is known...
  45. ANTIBODIES AS CATALYSTS
    Ian Wilson; Fiscal Year: 2004
    ..of abzymes that catalyze important classes of chemical reactions, cocaine-binding antibodies, and cocaine esterases in an iterative cycle of structure determination, redesign of ligands, and mutagenesis...
  46. ANALYSIS OF CYTOTOXIC T LYMPHOCYTE SERINE ESTERASE
    MARK PASTERNACK; Fiscal Year: 1991
    ..These granules contain perforin, as well as large quantities of a novel serine esterase (or esterases), the role of which is unknown...
  47. Catalytic specificity of zinc phosphate esterases investigated by evolution
    JONATHAN LASSILA; Fiscal Year: 2009
    ..Studying the mechanistic effects of changes in enzyme structure permits greater understanding of these essential biological catalysts that may permit the development of new enzymes with therapeutic and diagnostic value. ..
  48. PROCEDURES FOR PENETRATING THE BLOOD-BRAIN BARRIER
    VICTOR SHASHOUA; Fiscal Year: 1990
    ..Such transporter- coupled compounds behave as "prodrugs" which release their active moiety after hydrolysis by esterases following entry into the CNS...
  49. FLUORESCENT ACTIVATED CELL SORTING FOR ENZYME SCREENING
    Martin Keller; Fiscal Year: 2000
    ..RBI currently provides a number of enzyme libraries for various enzyme classes (Glycosidases; Esterases/Lipases; Aminotransferases; Phosphatases) suitable for screening...
  50. BETA 1 AND BETA 3 ADRENORECEPTORS
    JAMES GRANNEMAN; Fiscal Year: 2001
    ..Aim 2. To further characterize the differential signaling properties of beta1- beta3-AR and to examine the cellular and molecular basis. Specific Aim 3. To examine the molecular pharmacology of beta3-AR-selective agonists. ..
  51. Antiatherogenic Properties of tert-Butylhydroquinone
    William Stone; Fiscal Year: 2004
    ..Commercial development should be accelerated since NIH has already conducted extensive toxicological studies with TBHQ. ..
  52. Analysis of Therapeutic Adipose Tissue Remodeling
    JAMES GRANNEMAN; Fiscal Year: 2004
    ..2) To examine adipose tissue cellular dynamics during therapeutic remodeling induced by agonist activation of beta3-adrenergic receptors and PPAR-gamma. ..
  53. INNOVATIVE BIOMONITORING FOR LEAD IN SALIVA
    Charles Timchalk; Fiscal Year: 2006
    ..It is reasonable to speculate that once this model system has been adequately validated it can readily be employed to screen sensitive populations (e.g. children) that are at greatest risk from chronic Pb exposure. ..
  54. Inflammatory Genomics in Human Carotid Artery Disease
    GAIL JARVIK; Fiscal Year: 2006
    ..Genes that are implicated in disease may eventually allow targeted therapy. ..
  55. Development of Selective Nanoporous Sorbents for Radionuclide Decorporation
    Charles Timchalk; Fiscal Year: 2008
    ..abstract_text> ..
  56. Mixture Modeling: Pesticide Drug Interactions
    Charles Timchalk; Fiscal Year: 2009
    ..It is fully anticipated that this approach will facilitate the understanding of a variety of complex chemical interactions as it relates to the occupational health implications of working with insecticides. ..
  57. Prediction and Progression of Carotid Artery Disease
    GAIL JARVIK; Fiscal Year: 2009
    ..Aim 3: Pathway evaluation of oxidative loci and phenotypes in prediction of CAAD. ..
  58. The role of PKK in NFkappa B activation
    Shiv Pillai; Fiscal Year: 2007
    ..PKK represents a novel enzyme that may be critical in pathways that are important in cancer, inflammation, cell survival, and differentiation. ..
  59. Apolipoprotein-mediated cholesterol efflux
    W Davidson; Fiscal Year: 2008
    ..This understanding will form a basis for new interventions designed to enhance apolipoprotein-mediated cholesterol efflux from cells, particularly those within the atherosclerotic vessel wall. ..