- Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination
MONICA P COLAIACOVO
Department of Developmental Biology, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
Dev Cell 5:463-74. 2003
..Our data also suggest that early prophase barriers to utilization of sister chromatids as repair templates do not depend on central region assembly...
- Mechanism and control of meiotic recombination initiation
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, and Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
Curr Top Dev Biol 52:1-53. 2001
- Identification of genes encoding microbial glucuronoyl esterases
Xin Liang Li
National Center for Agricultural Utilization Research, USDA ARS, Peoria, IL 61604, USA
FEBS Lett 581:4029-35. 2007
..jecorina. The product of the cip2 gene was purified to homogeneity and shown to exhibit glucuronoyl esterase activity...
- Endonucleolytic processing of covalent protein-linked DNA double-strand breaks
Matthew J Neale
Molecular Biology Programs, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Nature 436:1053-7. 2005
..Our findings suggest a general mechanism for repair of protein-linked DSBs...
- Synapsis-dependent and -independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegans
Amy J MacQueen
Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305 5329, USA
Genes Dev 16:2428-42. 2002
- Molecular and biochemical characterization of a sand fly population from Sri Lanka: evidence for insecticide resistance due to altered esterases and insensitive acetylcholinesterase
S N Surendran
Department of Zoology, University of Jaffna, Jaffna, Sri Lanka
Bull Entomol Res 95:371-80. 2005
..There was preliminary evidence for elevated esterases and altered acetylcholinesterase in this population, the first report of these resistance mechanisms in sand ..
- Multiple biochemical and morphological factors underlie the production of methylketones in tomato trichomes
Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel
Plant Physiol 151:1952-64. 2009
..Furthermore, heterologous expression of MKS2 in Escherichia coli resulted in the production of methylketones in this host...
- Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity
Department of Biological Sciences, Northeast Structural Genomics Consortium, Columbia University, New York, NY 10027, USA
Proc Natl Acad Sci U S A 102:1773-8. 2005
..Our results suggest that SABP2 may be required to convert MeSA to SA as part of the signal transduction pathways that activate systemic acquired resistance and perhaps local defense responses as well...
- Recombinational DNA double-strand breaks in mice precede synapsis
S K Mahadevaiah
Division of Developmental Genetics, National Institute for Medical Research, London, UK
Nat Genet 27:271-6. 2001
..Loss of gamma-H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'...
- cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases
Section for Molecular Signalling, Department of Cell and Molecular Biology, Lund University, P O Box 94, S 221 00 Lund, Sweden
J Biol Chem 272:27218-23. 1997
..Northern blot analysis revealed that monoglyceride lipase is ubiquitously expressed among tissues, with a transcript size of about 4 kilobases...
- Microbial carbohydrate esterases deacetylating plant polysaccharides
Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia
Biotechnol Adv 30:1575-88. 2012
..The acetyl groups became targets of microbial carbohydrate esterases that evolved to overcome the complexity of the plant cell walls and that cooperate with glycoside hydrolases in ..
- Multiple insecticide resistance mechanisms involving metabolic changes and insensitive target sites selected in anopheline vectors of malaria in Sri Lanka
M Devika B Perera
Department of Zoology, Faculty of Science, University of Peradeniya, Peradeniya, Sri Lanka
Malar J 7:168. 2008
- The Serratia marcescens bioH gene encodes an esterase
Discovery Research Laboratory, Tanabe Seiyaku Co, Ltd, Kawagishi 2 chome, Toda, 335 8505, Saitama, Japan
Gene 302:185-92. 2003
..which belongs to the alpha/beta hydrolase-fold family comprising a wide variety of hydrolases including esterases. A catalytic triad composed of a nucleophilic residue (Ser80), an acidic residue (Asp206), and histidine (His234)..
- Catalytic promiscuity in the alpha/beta-hydrolase superfamily: hydroxamic acid formation, C--C bond formation, ester and thioester hydrolysis in the C--C hydrolase family
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
Chembiochem 9:71-6. 2008
..family of alpha/beta-hydrolases contains enzymes of several different catalytic activities, including esterases, C--C hydrolases and cofactor-independent haloperoxidases (perhydrolases), but the molecular basis of this ..
- Biochemical characterization and relative expression levels of multiple carbohydrate esterases of the xylanolytic rumen bacterium Prevotella ruminicola 23 grown on an ester-enriched substrate
Mirjam A Kabel
Royal Nedalco, Bergen op Zoom, The Netherlands
Appl Environ Microbiol 77:5671-81. 2011
We measured expression and used biochemical characterization of multiple carbohydrate esterases by the xylanolytic rumen bacterium Prevotella ruminicola 23 grown on an ester-enriched substrate to gain insight into the carbohydrate ..
- Isolation and characterization of cold-active family VIII esterases from an arctic soil metagenome
Eun Young Yu
Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, P O Box 107, Sinseongno 19, Yuseong, Daejeon 305 600, South Korea
Appl Microbiol Biotechnol 90:573-81. 2011
Functional screening for lipolytic enzymes at low temperatures resulted in the isolation of the novel cold-active esterases, EstM-N1 and EstM-N2, from a metagenomic DNA library of arctic soil samples...
- Ectopic expression of an esterase, which is a candidate for the unidentified plant cutinase, causes cuticular defects in Arabidopsis thaliana
Department of Botany, Graduate School of Science, Kyoto University, Kyoto, Japan
Plant Cell Physiol 51:123-31. 2010
..Our findings suggest that CDEF1 is a candidate gene for the unidentified plant cutinase...
- A thermostable esterase from Thermoanaerobacter tengcongensis opening up a new family of bacterial lipolytic enzymes
Chinese Academy of Sciences, Beijing, China
Biochim Biophys Acta 1814:1695-702. 2011
..Based on the enzymatic properties and sequence alignment we concluded that the esterase EstA3 represented a novel bacterial lipolytic enzyme group and in chronological order this group was assigned as Family XIV...
- The lipid droplet enzyme Tgl1p hydrolyzes both steryl esters and triglycerides in the yeast, Saccharomyces cerevisiae
Institute of Molecular Biosciences, SFB Biomembrane Research Center, University of Graz, Schubertstr 1, A8010 Graz, Austria
Biochim Biophys Acta 1735:50-8. 2005
..cerevisiae. In a tgl1 deletion mutant, the mobilization of steryl esters in vivo was delayed, but not abolished, suggesting the existence of additional enzymes involved in steryl ester mobilization...
- Screening and its potential application of lipolytic activity from a marine environment: characterization of a novel esterase from Yarrowia lipolytica CL180
Jun Tae Kim
Marine Biotechnology Center, Korea Ocean Research and Development Institute, Ansan, South Korea
Appl Microbiol Biotechnol 74:820-8. 2007
..This study represents a novel esterase of type B1 carboxylesterase/lipase family from a marine isolate, showing a potential usage as a biocatalyst because of enantioselectivity toward racemic ofloxacin ester...
- Novel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
FEMS Microbiol Ecol 72:228-37. 2010
..Furthermore, the whole sequence of fosmid pFL18 containing FLS18C and FLS18D was shotgun sequenced, and a total of 26 ORFs on it were analyzed and annotated...
- Growth-phase-dependent expression of the lipolytic system of Acinetobacter calcoaceticus BD413: cloning of a gene encoding one of the esterases
R G Kok
Department of Microbiology, E C Slater Institute, BioCentrum Amsterdam, The Netherlands
J Gen Microbiol 139:2329-42. 1993
..0 kDa. The amino acid sequence of this protein shows homology to a number of lipolytic enzymes, most notably to esterases. Deletion of estA only partially abolished cell-bound esterase activity in A...
- Paraoxonase active site required for protection against LDL oxidation involves its free sulfhydryl group and is different from that required for its arylesterase/paraoxonase activities: selective action of human paraoxonase allozymes Q and R
Lipid Research Laboratory, Technion Faculty of Medicine, The Rappaport Family Institute for Research in the Medical Sciences and Rambam Medical Center, Haifa, Israel
Arterioscler Thromb Vasc Biol 18:1617-24. 1998
- C. elegans HIM-17 links chromatin modification and competence for initiation of meiotic recombination
Kirthi C Reddy
Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
Cell 118:439-52. 2004
..Furthermore, DSB levels and incidence of chiasmata can be modulated by loss of LIN-35/Rb. These and other data suggest that chromatin state governs the timing of DSB competence...
- Enzymes from extremophiles
D C Demirjian
ThermoGen, Inc, Chicago, IL 60612, USA
Curr Opin Chem Biol 5:144-51. 2001
..This has also led to a greater understanding of stability factors involved in adaptation of these enzymes to their unusual environments...
- Plasma esterases and inflammation in ageing and frailty
Ruth E Hubbard
Department of Geriatric Medicine, School of Medicine, Cardiff University, 3rd Floor, Academic Centre, Llandough Hospital, Penarth, UK
Eur J Clin Pharmacol 64:895-900. 2008
b>Esterases are enzymes of drug metabolism known to be reduced in frail older people and during acute illness. The mechanism for this is unknown. The aim of this study was to examine esterase activity and inflammation in ageing and frailty.
- Structural reorganization and preorganization in enzyme active sites: comparisons of experimental and theoretically ideal active site geometries in the multistep serine esterase reaction cycle
Adam J T Smith
Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
J Am Chem Soc 130:15361-73. 2008
..The results provide a useful guiding principle for de novo design of enzymes with multistep mechanisms...
- Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from antarctic desert soil
Department of Biotechnology, University of the Western Cape, Cape Town, South Africa
Appl Environ Microbiol 75:4657-9. 2009
..The enzyme was active over a temperature range of 7 to 54 degrees C and at alkaline pH levels and is a potential candidate for industrial application...
- Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics
Department of Entomology and BIO5, University of Arizona, Tucson, Arizona 85721, USA
Annu Rev Entomol 52:231-53. 2007
..e., cytochrome P450 monooxygenases (P450s), esterases, and glutathione-S-transferases (GSTs), are the focus of this review...
- Paraoxonase 192 Gln-->Arg polymorphism: an independent risk factor for nonfatal arterial ischemic stroke among young adults
Whitaker Cardiovascular Institute, Evans Department of Medicine, Boston University School of Medicine, Boston, Mass 02118, USA
Stroke 33:1459-64. 2002
..The association of these polymorphisms with the risk of AIS remains controversial...
- High-resolution structure of ybfF from Escherichia coli K12: a unique substrate-binding crevice generated by domain arrangement
Suk Youl Park
Department of Chemistry and Institute of Basic Sciences, Chonnam National University, 300 Yongbong Dong, Buk Gu, Gwangju 500 757, Korea
J Mol Biol 376:1426-37. 2008
b>Esterases are one of the most common enzymes and are involved in diverse cellular functions...
- Cold-active esterase from Psychrobacter sp. Ant300: gene cloning, characterization, and the effects of Gly-->Pro substitution near the active site on its catalytic activity and stability
Laboratory of Microbial Biochemistry, Institute for Chemical Research, Kyoto University, Uji, Kyoto fu, Kyoto 611 0011, Japan
Biochim Biophys Acta 1696:59-65. 2004
- Relationship between age and plasma esterases
Department of Geriatric Medicine, Academic Centre, Llandough Hospital, Penarth, UK
Age Ageing 30:41-5. 2001
..RESULTS: we found no correlation between the activities of any of the enzymes measured and advancing age. CONCLUSION: age per se is not associated with reductions in the activities of esterase enzymes...
- Functional expression and refolding of new alkaline esterase, EM2L8 from deep-sea sediment metagenome
Hyo Jung Park
Division of Biotechnology, The Catholic University of Korea, 43 1 Yokkok 2 dong, Wonmi gu, Bucheon, Gyeonggido 420 743, South Korea
Protein Expr Purif 52:340-7. 2007
..Moreover, it was found to have an optimum pH of 10-11, thus revealing that it is an alkaline enzyme. In this paper, the new esterase EM2L8 buried in a deep-sea sediment became known on the surface and was characterized biochemically...
- A thermostable feruloyl-esterase from the hemicellulolytic bacterium Thermobacillus xylanilyticus releases phenolic acids from non-pretreated plant cell walls
UMR614 Fractionnement des AgroRessources et Environnement, INRA, 51686 Reims, France
Appl Microbiol Biotechnol 90:541-52. 2011
..4 kDa, similar to feruloyl-esterases from cellulolytic bacteria and fungi...
- Characterization of digestive gland esterase-lipase activity of juvenile redclaw crayfish Cherax quadricarinatus
Laboratorio de Fisiología y Bioquímica Comparada, Centro de Investigaciones Biologicas del Noroeste, Mar Bermejo 195, Col Playa Palo Santa Rita, La Paz, BCS 23090, Mexico
Comp Biochem Physiol B Biochem Mol Biol 135:337-47. 2003
..The enzyme activity diminished as the length of the fatty acid chain of substrates increased. Molecular masses for four isozymes were 43, 46, 63 and 118 kDa, respectively, as determined by PAGE...
- MRI cerebral white matter lesions and paraoxonase PON1 polymorphisms : three-year follow-up of the austrian stroke prevention study
Department of Neurology, Karl Franzens University, Graz, Austria
Arterioscler Thromb Vasc Biol 20:1811-6. 2000
..05/mm Hg), and LL paraoxonase genotype (odds ratio, 2. 65) to be significant predictors of WML progression. These data suggest that the LL PON1 genotype at position 54 influences the extent and progression of WMLs in elderly subjects...
- Two glucuronoyl esterases of Phanerochaete chrysosporium
Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dubravska cesta 9, 845 38, Bratislava, Slovakia
Arch Microbiol 191:133-40. 2009
..Comparison of the amino acid sequences of identified and putative glucuronoyl esterases to other types of carbohydrate esterases (CE) confirmed that they form a separate family of CEs...
- Identification of two novel esterases from a marine metagenomic library derived from South China Sea
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230027, China
Appl Microbiol Biotechnol 80:615-25. 2008
..were isolated from a marine microbial metagenomic library by functional screening, and the corresponding esterases EstA and EstB were biochemically characterized...
- Cloning, recombinant expression and biochemical characterisation of novel esterases from Bacillus sp. associated with the marine sponge Aplysina aerophoba
Institut für Technische Biochemie, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
Appl Microbiol Biotechnol 67:59-69. 2005
Two novel esterases (EstB1 and EstB2) were isolated from a genomic library of Bacillus sp. associated with the marine sponge Aplysina aerophoba...
- Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system
Department of Pharmaceutical Biology, Institute of Pharmacy, Johannes Gutenberg University, Staudinger Weg 5, 55128, Mainz, Germany
Planta 222:888-98. 2005
..This expression allowed molecular analysis of AAE for the first time and increased the number of functionally expressed alkaloid genes from Rauvolfia now to eight, and the number of ajmaline pathway-specific cDNAs to a total of six...
- Characterization and its potential application of two esterases derived from the arctic sediment metagenome
Jeong Ho Jeon
Marine Biotechnology Research Centre, Korean Ocean Research and Development Institute, Ansan, South Korea
Mar Biotechnol (NY) 11:307-16. 2009
..The esterases genes were overexpressed in Escherichia coli by co-expressing GroEL-GroES chaperonine, and the recombinant ..
- Purification and cloning of an esterase from the weed black-grass (Alopecurus myosuroides), which bioactivates aryloxyphenoxypropionate herbicides
School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, UK
Plant J 39:894-904. 2004
- Purification of ENOD8 proteins from Medicago sativa root nodules and their characterization as esterases
Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104, USA
Plant Physiol Biochem 42:73-9. 2004
..Considering ENOD8 protein sequence similarity to an exopolygalacturonase/EP4/iEP4 and lanatoside 15'-O-acetylesterase with the results presented here predicts that ENOD8 substrates could be acetylated oligo- or polysaccharides...
- Isolation and characterization of the early nodule-specific protein homologue (Hev b 13), an allergenic lipolytic esterase from Hevea brasiliensis latex
Siti Arija M Arif
Rubber Research Institute of Malaysia, 50450 Kuala Lumpur, Malaysia
J Biol Chem 279:23933-41. 2004
..The purified protein showed lipase and esterase activities and may be involved in plant defense...
- Identification and biochemical characterization of a GDSL-motif carboxylester hydrolase from Carica papaya latex
CNRS, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse, Marseille, France
Biochim Biophys Acta 1791:1048-56. 2009
- Characterization of novel esterases in insecticide-resistant mosquitoes
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Insect Biochem Mol Biol 37:1131-7. 2007
..detected Ester alleles, Ester(B10) and Ester(11) (including associated Ester(A11) and Ester(B11)), coding for esterases B10 and A11-B11, respectively, are characterized qualitatively and quantitatively...
- Indication for a new lipolytic enzyme family: isolation and characterization of two esterases from a metagenomic library
Institute of Biochemistry, University of Leipzig, Germany
J Mol Microbiol Biotechnol 18:181-7. 2010
..EstGK1 and EstZ3 have only minor overall sequence similarity to known esterases. We propose that, together with other hypothetical enzymes, they constitute a new family of lipolytic enzymes...
- Gene cloning and characterization of a novel thermophilic esterase from Fervidobacterium nodosum Rt17-B1
Jilin University, Changchun, China
Acta Biochim Biophys Sin (Shanghai) 42:288-95. 2010
..high with a half-life of 80 min at 70 degrees C, but less stable compared with some other hyperthermophilic esterases. A structural model was constructed using acetylesterase from Aspergillus aculeatus as a template...
- Production and characterization of two N-terminal truncated esterases from Thermus thermophilus HB27 in a mesophilic yeast: effect of N-terminus in thermal activity and stability
Department of Analytical and Food Chemistry, University of Vigo, Campus of Ourense, As Lagoas, 32004 Ourense, Spain
Protein Expr Purif 78:120-30. 2011
..Minor differences were observed in the substrate specificity. Our results suggest that the N-terminal segment is critical for maintaining the hyperthermophilic function and stability...
- Characterization of two metagenome-derived esterases that reactivate chloramphenicol by counteracting chloramphenicol acetyltransferase
Department of Medical Bioscience, Dong A University, Busan 604 714, Korea
J Microbiol Biotechnol 21:1203-10. 2011
..we investigated lipolytic enzymes selected from an alluvial soil metagenomic library, and identified two novel esterases, EstDL26 and EstDL136...
- Identification of human cytochrome P450 isoforms and esterases involved in the metabolism of mirabegron, a potent and selective β3-adrenoceptor agonist
Drug Metabolism Research Laboratories, Astellas Pharma Inc, Osaka, Japan
Xenobiotica 42:957-67. 2012
1. Human cytochrome P450 (CYP) enzymes and esterases involved in the metabolism of mirabegron, a potent and selective human β(3)-adrenoceptor agonist intended for the treatment of overactive bladder, were identified in in vitro studies...
- Biochemical and physiological studies of soluble esterases from Drosophila melanogaster
M J Healy
CSIRO Division of Entomology, Canberra, ACT, Australia
Biochem Genet 29:365-88. 1991
Twenty-two soluble esterases have been identified in D. melanogaster by combining the techniques of native polyacrylamide gel electrophoresis and isoelectric focusing...
- Esterases from Bacillus subtilis and B. stearothermophilus share high sequence homology but differ substantially in their properties
Institute of Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstrasse 16, 17487 Greifswald, Germany
Appl Microbiol Biotechnol 60:320-6. 2002
..Despite the high homology, both esterases revealed remarkable differences in their properties...
- Functional proteomic analysis of lipases and esterases in cultured human adipocytes
Institute of Biochemistry, Graz University of Technology, Graz, Austria
J Proteome Res 9:6334-44. 2010
..Both of them showed pronounced hydrolytic activities on hydrophobic acylglycerols and therefore may be considered lipases. The physiological functions of the novel lipolytic proteins in vivo are currently subject to investigation...
- Overexpressed esterases in a fenvalerate resistant strain of the cotton bollworm, Helicoverpa armigera
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects Ministry of Agriculture, Nanjing 210095, China
Insect Biochem Mol Biol 41:14-21. 2011
..with four putative carboxylesterase genes, all of which clustered within a phylogenetic clade of secreted midgut esterases. Quantitative PCR on these four genes showed several-fold greater expression in tissues of YGF compared to SCD ..
- A novel esterase from Bacillus subtilis (RRL 1789): purification and characterization of the enzyme
Regional Research Laboratory, Canal Road, Jammu Tawi 180001, India
Protein Expr Purif 45:262-8. 2006
..The N-terminal amino acid sequence of BSE determined, as Met-Thr-Pro-Glu-Iso-Val-Thr-Thr-Glu-Tyr-Gly- revealed similarity with the N-terminal amino acid sequence of p-nitrobenzylesterase of B. subtilis...
- Functions of the siderophore esterases IroD and IroE in iron-salmochelin utilization
Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
Microbiology 151:2363-72. 2005
..It is suggested that homologues in other bacteria are also periplasmic IroE-type esterases of siderophores.
- SPO11 is required for sex-body formation, and Spo11 heterozygosity rescues the prophase arrest of Atm-/- spermatocytes
Marina A Bellani
Genetics and Biochemistry Branch, NIDDK, NIH, Bethesda, MD 20892, USA
J Cell Sci 118:3233-45. 2005
- Effects of four host plants on susceptibility of Spodoptera litura (Lepidoptera: Noctuidae) larvae to five insecticides and activities of detoxification esterases
Department of Entomology, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, China
Pest Manag Sci 66:1273-9. 2010
..The susceptibility of S. litura larvae reared on tobacco, Chinese cabbage, cowpea and sweet potato to phoxim, chlorfenapyr, methomyl, fenvalerate and emamectin benzoate under laboratory conditions was determined...
- The classification of esterases: an important gene family involved in insecticide resistance--a review
Isabela Reis Montella
Laboratório de Fisiologia e Controle de Artrópodes Vetores, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brazil
Mem Inst Oswaldo Cruz 107:437-49. 2012
..Historically, insecticide resistance has driven research on insect esterases and schemes for their classification...
- The SGNH-hydrolase of Streptomyces coelicolor has (aryl)esterase and a true lipase activity
Laboratory of Molecular Genetics, Division of Molecular Biology, Ruder Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia
Biochimie 91:390-400. 2009
..coelicolor SGNH-hydrolase generated three distinct groups consisting of proteins from Actinomycetales, Ascomycota and Nematoda. At present it seems that these enzymes are most conserved among soil inhabiting organisms...
- Genetically-determined polymorphism of nonspecific esterases and phosphoglucomutase in eight introduced breeds of the silkworm, Bombyx mori, raised in Bulgaria
Department of Developmental Biology, Section of Genetics, Faculty of Biology, University of Plovdiv, 4000 Plovdiv, Bulgaria
J Insect Sci 8:18. 2008
..Polymorphism of nonspecific esterases from pupal haemolymph was analyzed, as well as of phosphoglucomutase from different organs of larvae, pupae and ..
- The Lipase Engineering Database: a navigation and analysis tool for protein families
Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, D 70569 Stuttgart, Germany
Nucleic Acids Res 31:319-21. 2003
..led.uni-stuttgart.de) integrates information on sequence, structure, and function of lipases, esterases, and related proteins...
- An exocellular protein from the oil-degrading microbe Acinetobacter venetianus RAG-1 enhances the emulsifying activity of the polymeric bioemulsifier emulsan
Department of Molecular Microbiology and Biotechnology, George S Wise Faculty of Life Science, Tel Aviv University, Tel Aviv 69978, Israel
Appl Environ Microbiol 69:2608-15. 2003
..Other proteins, including a His-tagged overexpressed esterase from the related organism Acinetobacter calcoaceticus BD4, showed no enhancement...
- Activity of lipases and esterases towards tertiary alcohols: insights into structure-function relationships
Institute of Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstr 16, 17487 Greifswald, Germany
Angew Chem Int Ed Engl 41:3211-3. 2002
- In vitro stability of methylmethacrylic acid, TEGDMA and HEMA exposed to esterases
Walther Straub Institute of Pharmacology and Toxicology, Ludwig Maximilians University of Munich, 80336 Munich, Germany
Dent Mater 25:1044-9. 2009
- Risk of myocardial infarction associated with Gln/Arg 192 polymorphism in the human paraoxonase gene and diabetes mellitus. The REGICOR Investigators
Lipids and Cardiovascular Epidemiology Unit, Institut Municipal d Investigacio Medica, IMIM, Barcelona, Spain
Eur Heart J 21:33-8. 2000
- Release of short chain fatty acids from cream lipids by commercial lipases and esterases
Laboratory of Industrial Microbiology and Biocatalysis, Faculty of Bioscience Engineering, Ghent University, Belgium
Biotechnol Lett 30:311-5. 2008
Lipases and esterases are frequently used in dairy production processes to enhance the buttery flavour of the end product...
- Cloning and expression in Escherichia coli of an esterase-coding gene from the oil-degrading bacterium Acinetobacter calcoaceticus RAG-1
P G Reddy
Department of Microbiology, George S Wise Faculty of Life Sciences, Tel Aviv University, Israel
Gene 76:145-52. 1989
..The fact that the production of active esterase depended on the orientation of the est gene within the vector suggested that transcription proceeded from the tet promoter in pBR322...
- Characterization of a novel thermostable esterase from Thermus scotoductus SA-01: evidence of a new family of lipolytic esterases
Erika M du Plessis
CSIR Biosciences, Box 395, Pretoria, 0001, Gauteng, South Africa
Curr Microbiol 60:248-53. 2010
..Sequence analysis showed that both proteins contained the G-X-S-X-G signature sequence motif present in most esterases and lipases...
- Production of flavour compounds from fat during cheese ripening by action of lipases and esterases
Irma Verónica Wolf
Instituto de Lactología Industrial, Facultad de Ingenieria Quimica, Universidad Nacional del Litoral CONICET Consejo Nacional de Investigaciones Científicas y Técnicas, Santiago del Estero 2829, S3000AOM, Santa Fe, Argentina
Protein Pept Lett 16:1235-43. 2009
..Before starting with the specific study about formation of flavour compounds from milk fat during cheese ripening, a brief review of the technological aspects of cheese production is needed...
- Novel thermophilic and thermostable lipolytic enzymes from a Thailand hot spring metagenomic library
BIOTEC Central Research Unit, National Center for Genetic Engineering and Biotechnology, 113 National Science Park, Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand
J Biotechnol 133:42-9. 2008
..The isolated enzymes, are therefore, different from other known patatin-like phospholipases and esterases, which usually show no activity for substrates longer than C(10)...
- Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism
Molecular Biology Program, Memorial Sloan Kettering Cancer Center and Weill Graduate School of Medical Sciences of Cornell University, 1275 York Avenue, Box 97, New York, NY 10021, USA
Mol Cell 13:549-59. 2004
..Ski8 works with Spo11 to recruit other DSB proteins to meiotic chromosomes, implicating Ski8 as a scaffold protein mediating assembly of a multiprotein complex essential for DSB formation...
- Sex increases the efficacy of natural selection in experimental yeast populations
Matthew R Goddard
NERC Centre for Population Biology and Department of Biological Sciences, Imperial College London, Silwood Park Campus, Ascot SL5 7PY, UK
Nature 434:636-40. 2005
..We show that, as predicted by the theory, sex increases the rate of adaptation to a new harsh environment but has no measurable effect on fitness in a new benign environment where there is little selection...
- Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY 10021, USA
Chromosoma 116:471-86. 2007
..These studies also suggest that an essential function of Rec102 and Rec104 is to connect Mei4 and Rec114 to Spo11...
- Differential gene expression of mammalian SPO11/TOP6A homologs during meiosis
Molecular and Structural Biology Division, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, CA 94550, USA
FEBS Lett 462:329-34. 1999
..2-13.3, a region that is amplified in some breast cancers. Sequence homology and differential expression together support a highly conserved role for SPO11 in the enzymatic cleavage of DNA that accompanies meiotic recombination...
- Inter-individual variability in esterases in human liver
Toxicology Unit, School of Clinical and Laboratory Sciences, 4th Floor, Devonshire Building, Devonshire Terrace, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4EA, UK
Biochem Pharmacol 74:932-9. 2007
..Lower correlations with larger alcohol leaving group parabens are consistent with more hCE2 involvement...
- The role of esterases in the metabolism of ciclesonide to desisobutyryl-ciclesonide in human tissue
Toxicology Unit, Clinical and Laboratory Sciences, The University of Newcastle, Newcastle upon Tyne, UK
Biochem Pharmacol 73:1657-64. 2007
Ciclesonide (CIC) is an inhaled glucocorticosteroid. This study aimed to identify esterases involved in the metabolism of CIC to the active metabolite desisobutyryl-ciclesonide (des-CIC), and to measure hydrolysis rates in human liver, ..
- Esterase 2-oligodeoxynucleotide conjugates as sensitive reporter for electrochemical detection of nucleic acid hybridization
Laboratorium fur Biochemie, Universitat Bayreuth, Universitätsstr 30, 95440 Bayreuth, Germany
Biosens Bioelectron 22:1798-806. 2007
..Artificial single nucleotide mismatches in a 510-nucleotide ssDNA could be reliably determined using esterase 2-oligodeoxynucleotide conjugates as a reporter...
- Methods to increase enantioselectivity of lipases and esterases
Uwe T Bornscheuer
Institute of Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstrasse 16, D 17487, Greifswald, Germany
Curr Opin Biotechnol 13:543-7. 2002
Lipases and esterases are frequently used in the synthesis of optically pure compounds; however, natural enzymes do not always show sufficiently high enantioselectivity...
- Rapid degradation of oseltamivir phosphate in clinical samples by plasma esterases
Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok 10400, Thailand
Antimicrob Agents Chemother 50:3197-9. 2006
The anti-influenza drug oseltamivir is an ester prodrug activated by hepatic carboxylesterases. Plasma esterases also convert up to 31...
- Chaperone-like activities of alpha-synuclein: alpha-synuclein assists enzyme activities of esterases
Division of Biotechnology and Molecular Engineering, College of Engineering, Ajou University, Suwon 443 749, Republic of Korea
Biochem Biophys Res Commun 346:1142-9. 2006
..which has no well-defined secondary or tertiary structure, can protect the enzyme activity of microbial esterases against stress conditions such as heat, pH, and organic solvents...
- GDSL family of serine esterases/lipases
Casimir C Akoh
Department of Food Science and Technology, University of Georgia, Athens, GA 30602 7610, USA
Prog Lipid Res 43:534-52. 2004
GDSL esterases and lipases are hydrolytic enzymes with multifunctional properties such as broad substrate specificity and regiospecificity...
- Biochemical detection of pyrethroid resistance mechanisms in Anopheles minimus in Thailand
Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
J Vector Ecol 28:108-16. 2003
..Expression of levels of non-specific esterases, monooxygenases, and glutathione S-transferases (GSTs) were measured...
- Isolation and biochemical characterization of two novel metagenome-derived esterases
Molekulare Enzymtechnologie, Universitat Duisburg Essen, Lotharstrasse 1, 47057 Duisburg, Germany
Appl Environ Microbiol 72:3637-45. 2006
..Both esterases are approximately 380 amino acids in size and show similarity to beta-lactamases, indicating that they belong to ..
- Role of the N terminus in enzyme activity, stability and specificity in thermophilic esterases belonging to the HSL family
Institute of Protein Biochemistry, CNR, Via P Castellino 111, 80131 Napoli, Italy
J Mol Biol 345:501-12. 2005
..This is the first report highlighting the importance of the "cap" domain in the HSL family, since the N terminus partly contributes to the building up of this structure...
- The effect of piperonyl butoxide on pyrethroid-resistance-associated esterases in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)
Susan J Young
Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK
Pest Manag Sci 61:397-401. 2005
..Biochemical studies have shown that pyrethroid-resistance-associated esterases in H armigera are inhibited by the insecticide synergist piperonyl butoxide (PBO)...
- Distinct DNA-damage-dependent and -independent responses drive the loss of oocytes in recombination-defective mouse mutants
Monica Di Giacomo
Molecular Biology Program, Memorial Sloan Kettering Cancer Center and Weill Graduate School of Medical Sciences of Cornell University, 1275 York Avenue, New York, NY 10021, USA
Proc Natl Acad Sci U S A 102:737-42. 2005
..The absence of ATM caused defects in folliculogenesis that were similar to those in Dmc1 mutants and that could be suppressed by Spo11 mutation, implying that oocyte death in Atm-deficient animals is a response to defective DSB repair...
- Comparative acaricide susceptibility and detoxifying enzyme activities in field-collected resistant and susceptible strains of Tetranychus urticae
Thomas Van Leeuwen
Laboratory of Agrozoology, Department of Crop Protection, Faculty of Agricultural and Applied Biological Science, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
Pest Manag Sci 61:499-507. 2005
..Enhanced detoxification by increased activity of mono-oxygenases (MO) and esterases is at least partially responsible for the observed resistance and cross-resistance...
- New thermophilic and thermostable esterase with sequence similarity to the hormone-sensitive lipase family, cloned from a metagenomic library
Jin Kyu Rhee
Department of Biotechnology, Yonsei University, 134 Sinchon dong, Seodaemun gu, Seoul 120 749, Korea
Appl Environ Microbiol 71:817-25. 2005
..An amino acid sequence comparison with other esterases and lipases revealed that the enzyme should be classified as a new member of the hormone-sensitive lipase family...
- Roles played by esterase activity and by a sodium channel mutation involved in pyrethroid resistance in populations of Boophilus microplus (Acari: Ixodidae) collected from Yucatan, Mexico
Centro Nacional de Investigaciones en Parasitología Veterinaria INIFAP, Morelos, Mexico
J Med Entomol 42:1020-5. 2005
..It was not determined whether enhanced esterase-based hydrolytic activity was involved in pyrethroid resistance in the populations tested...
- Biochemical profiling in silico--predicting substrate specificities of large enzyme families
Institute of Technical Biochemistry, University of Stuttgart, Germany
J Biotechnol 124:108-16. 2006
..specificity of cholinesterases toward acetyl- and butyrylcholine, the changes of activity of lipases and esterases upon the size of the acid moieties, activity of lipases and esterases toward tertiary alcohols, and the ..
- Strategic selection of hyperthermophilic esterases for resolution of 2-arylpropionic esters
Amitabh C Sehgal
North Carolina State University, Department of Chemical Engineering, Raleigh, North Carolina 27695 7905, USA
Biotechnol Prog 19:1410-6. 2003
- MELDB: a database for microbial esterases and lipases
Ho Young Kang
Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, KRIBB, P O Box 115, Yuseong, Daejeon 305 600, Republic of Korea
FEBS Lett 580:2736-40. 2006
MELDB is a comprehensive protein database of microbial esterases and lipases which are hydrolytic enzymes important in the modern industry...
- Detecting cellulase and esterase enzyme activities encoded by novel genes present in environmental DNA libraries
Helen C Rees
Department of Microbiology and Immunology, University of Leicester, University Road, Leicester, LE1 9HN, UK
Extremophiles 7:415-21. 2003
..Esterase/lipase-positive clones from Lake Elmenteita were isolated with a frequency of 1 in 30,000 from a library made from an olive-oil enrichment as compared to 1 in 100,000 from an environmental library...
- Identification of a point mutation in an esterase gene in different populations of the southern cattle tick, Boophilus microplus
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station 77843 4467, USA
Insect Biochem Mol Biol 30:969-77. 2000
..Identification of this mutation and the strong signal detected in southern blot with this strain, suggest that esterases are contributing to detoxification of pyrethroid compounds, as a resistant mechanism in Mexican strains of the ..
- Current progress on esterases: from molecular structure to function
Biomedical Research Institute, Chiba University, Chiba, Japan
Drug Metab Dispos 30:488-93. 2002
..This article covers mechanisms of regulation of gene expression of the various esterases by developmental factors and xenobiotics, as well as the interplay between physiological and chemical regulation ..
- The mouse Spo11 gene is required for meiotic chromosome synapsis
P J Romanienko
Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health 20892, Bethesda, MD, USA
Mol Cell 6:975-87. 2000
..We speculate that there is an additional role for Spo11, after it generates DSBs, in synapsis...
- Identification of residues in yeast Spo11p critical for meiotic DNA double-strand break formation
Robert L Diaz
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Mol Cell Biol 22:1106-15. 2002
..These results are consistent with a multimeric structure for Spo11p in vivo but may also indicate that the amount of Spo11 protein is not a limiting factor for DSB formation in normal cells...
- Screening, nucleotide sequence, and biochemical characterization of an esterase from Pseudomonas fluorescens with high activity towards lactones
Institute for Technical Biochemistry, University of Stuttgart, 70569 Stuttgart, Germany
Appl Environ Microbiol 65:477-82. 1999
..8 U/mg) to delta-valerolactone (36.5 U/mg). The activities towards the aliphatic esters were significantly lower; the only exception was the activity toward ethyl caprylate, which was the preferred substrate...