esterases

Summary

Top Publications

  1. ncbi Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination
    MONICA P COLAIACOVO
    Department of Developmental Biology, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Dev Cell 5:463-74. 2003
  2. ncbi Mechanism and control of meiotic recombination initiation
    S Keeney
    Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, and Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Curr Top Dev Biol 52:1-53. 2001
  3. ncbi Identification of genes encoding microbial glucuronoyl esterases
    Xin Liang Li
    National Center for Agricultural Utilization Research, USDA ARS, Peoria, IL 61604, USA
    FEBS Lett 581:4029-35. 2007
  4. pmc Endonucleolytic processing of covalent protein-linked DNA double-strand breaks
    Matthew J Neale
    Molecular Biology Programs, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 436:1053-7. 2005
  5. pmc Synapsis-dependent and -independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegans
    Amy J MacQueen
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305 5329, USA
    Genes Dev 16:2428-42. 2002
  6. ncbi Molecular and biochemical characterization of a sand fly population from Sri Lanka: evidence for insecticide resistance due to altered esterases and insensitive acetylcholinesterase
    S N Surendran
    Department of Zoology, University of Jaffna, Jaffna, Sri Lanka
    Bull Entomol Res 95:371-80. 2005
  7. pmc Multiple biochemical and morphological factors underlie the production of methylketones in tomato trichomes
    Imri Ben-Israel
    Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel
    Plant Physiol 151:1952-64. 2009
  8. pmc Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity
    Farhad Forouhar
    Department of Biological Sciences, Northeast Structural Genomics Consortium, Columbia University, New York, NY 10027, USA
    Proc Natl Acad Sci U S A 102:1773-8. 2005
  9. ncbi Recombinational DNA double-strand breaks in mice precede synapsis
    S K Mahadevaiah
    Division of Developmental Genetics, National Institute for Medical Research, London, UK
    Nat Genet 27:271-6. 2001
  10. ncbi cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases
    M Karlsson
    Section for Molecular Signalling, Department of Cell and Molecular Biology, Lund University, P O Box 94, S 221 00 Lund, Sweden
    J Biol Chem 272:27218-23. 1997

Detail Information

Publications241 found, 100 shown here

  1. ncbi Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination
    MONICA P COLAIACOVO
    Department of Developmental Biology, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Dev Cell 5:463-74. 2003
    ..Our data also suggest that early prophase barriers to utilization of sister chromatids as repair templates do not depend on central region assembly...
  2. ncbi Mechanism and control of meiotic recombination initiation
    S Keeney
    Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, and Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Curr Top Dev Biol 52:1-53. 2001
    ....
  3. ncbi Identification of genes encoding microbial glucuronoyl esterases
    Xin Liang Li
    National Center for Agricultural Utilization Research, USDA ARS, Peoria, IL 61604, USA
    FEBS Lett 581:4029-35. 2007
    ..jecorina. The product of the cip2 gene was purified to homogeneity and shown to exhibit glucuronoyl esterase activity...
  4. pmc Endonucleolytic processing of covalent protein-linked DNA double-strand breaks
    Matthew J Neale
    Molecular Biology Programs, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 436:1053-7. 2005
    ..Our findings suggest a general mechanism for repair of protein-linked DSBs...
  5. pmc Synapsis-dependent and -independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegans
    Amy J MacQueen
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305 5329, USA
    Genes Dev 16:2428-42. 2002
    ....
  6. ncbi Molecular and biochemical characterization of a sand fly population from Sri Lanka: evidence for insecticide resistance due to altered esterases and insensitive acetylcholinesterase
    S N Surendran
    Department of Zoology, University of Jaffna, Jaffna, Sri Lanka
    Bull Entomol Res 95:371-80. 2005
    ..There was preliminary evidence for elevated esterases and altered acetylcholinesterase in this population, the first report of these resistance mechanisms in sand ..
  7. pmc Multiple biochemical and morphological factors underlie the production of methylketones in tomato trichomes
    Imri Ben-Israel
    Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel
    Plant Physiol 151:1952-64. 2009
    ..Furthermore, heterologous expression of MKS2 in Escherichia coli resulted in the production of methylketones in this host...
  8. pmc Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity
    Farhad Forouhar
    Department of Biological Sciences, Northeast Structural Genomics Consortium, Columbia University, New York, NY 10027, USA
    Proc Natl Acad Sci U S A 102:1773-8. 2005
    ..Our results suggest that SABP2 may be required to convert MeSA to SA as part of the signal transduction pathways that activate systemic acquired resistance and perhaps local defense responses as well...
  9. ncbi Recombinational DNA double-strand breaks in mice precede synapsis
    S K Mahadevaiah
    Division of Developmental Genetics, National Institute for Medical Research, London, UK
    Nat Genet 27:271-6. 2001
    ..Loss of gamma-H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'...
  10. ncbi cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases
    M Karlsson
    Section for Molecular Signalling, Department of Cell and Molecular Biology, Lund University, P O Box 94, S 221 00 Lund, Sweden
    J Biol Chem 272:27218-23. 1997
    ..Northern blot analysis revealed that monoglyceride lipase is ubiquitously expressed among tissues, with a transcript size of about 4 kilobases...
  11. doi Microbial carbohydrate esterases deacetylating plant polysaccharides
    Peter Biely
    Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia
    Biotechnol Adv 30:1575-88. 2012
    ..The acetyl groups became targets of microbial carbohydrate esterases that evolved to overcome the complexity of the plant cell walls and that cooperate with glycoside hydrolases in ..
  12. pmc Multiple insecticide resistance mechanisms involving metabolic changes and insensitive target sites selected in anopheline vectors of malaria in Sri Lanka
    M Devika B Perera
    Department of Zoology, Faculty of Science, University of Peradeniya, Peradeniya, Sri Lanka
    Malar J 7:168. 2008
    ....
  13. ncbi The Serratia marcescens bioH gene encodes an esterase
    Hiroyuki Akatsuka
    Discovery Research Laboratory, Tanabe Seiyaku Co, Ltd, Kawagishi 2 chome, Toda, 335 8505, Saitama, Japan
    Gene 302:185-92. 2003
    ..which belongs to the alpha/beta hydrolase-fold family comprising a wide variety of hydrolases including esterases. A catalytic triad composed of a nucleophilic residue (Ser80), an acidic residue (Asp206), and histidine (His234)..
  14. ncbi Catalytic promiscuity in the alpha/beta-hydrolase superfamily: hydroxamic acid formation, C--C bond formation, ester and thioester hydrolysis in the C--C hydrolase family
    Chen Li
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    Chembiochem 9:71-6. 2008
    ..family of alpha/beta-hydrolases contains enzymes of several different catalytic activities, including esterases, C--C hydrolases and cofactor-independent haloperoxidases (perhydrolases), but the molecular basis of this ..
  15. pmc Biochemical characterization and relative expression levels of multiple carbohydrate esterases of the xylanolytic rumen bacterium Prevotella ruminicola 23 grown on an ester-enriched substrate
    Mirjam A Kabel
    Royal Nedalco, Bergen op Zoom, The Netherlands
    Appl Environ Microbiol 77:5671-81. 2011
    We measured expression and used biochemical characterization of multiple carbohydrate esterases by the xylanolytic rumen bacterium Prevotella ruminicola 23 grown on an ester-enriched substrate to gain insight into the carbohydrate ..
  16. doi Isolation and characterization of cold-active family VIII esterases from an arctic soil metagenome
    Eun Young Yu
    Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, P O Box 107, Sinseongno 19, Yuseong, Daejeon 305 600, South Korea
    Appl Microbiol Biotechnol 90:573-81. 2011
    Functional screening for lipolytic enzymes at low temperatures resulted in the isolation of the novel cold-active esterases, EstM-N1 and EstM-N2, from a metagenomic DNA library of arctic soil samples...
  17. ncbi Ectopic expression of an esterase, which is a candidate for the unidentified plant cutinase, causes cuticular defects in Arabidopsis thaliana
    Kentaro Takahashi
    Department of Botany, Graduate School of Science, Kyoto University, Kyoto, Japan
    Plant Cell Physiol 51:123-31. 2010
    ..Our findings suggest that CDEF1 is a candidate gene for the unidentified plant cutinase...
  18. doi A thermostable esterase from Thermoanaerobacter tengcongensis opening up a new family of bacterial lipolytic enzymes
    Lang Rao
    Chinese Academy of Sciences, Beijing, China
    Biochim Biophys Acta 1814:1695-702. 2011
    ..Based on the enzymatic properties and sequence alignment we concluded that the esterase EstA3 represented a novel bacterial lipolytic enzyme group and in chronological order this group was assigned as Family XIV...
  19. ncbi The lipid droplet enzyme Tgl1p hydrolyzes both steryl esters and triglycerides in the yeast, Saccharomyces cerevisiae
    Anita Jandrositz
    Institute of Molecular Biosciences, SFB Biomembrane Research Center, University of Graz, Schubertstr 1, A8010 Graz, Austria
    Biochim Biophys Acta 1735:50-8. 2005
    ..cerevisiae. In a tgl1 deletion mutant, the mobilization of steryl esters in vivo was delayed, but not abolished, suggesting the existence of additional enzymes involved in steryl ester mobilization...
  20. ncbi Screening and its potential application of lipolytic activity from a marine environment: characterization of a novel esterase from Yarrowia lipolytica CL180
    Jun Tae Kim
    Marine Biotechnology Center, Korea Ocean Research and Development Institute, Ansan, South Korea
    Appl Microbiol Biotechnol 74:820-8. 2007
    ..This study represents a novel esterase of type B1 carboxylesterase/lipase family from a marine isolate, showing a potential usage as a biocatalyst because of enantioselectivity toward racemic ofloxacin ester...
  21. ncbi Novel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment
    Yongfei Hu
    Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    FEMS Microbiol Ecol 72:228-37. 2010
    ..Furthermore, the whole sequence of fosmid pFL18 containing FLS18C and FLS18D was shotgun sequenced, and a total of 26 ORFs on it were analyzed and annotated...
  22. ncbi Growth-phase-dependent expression of the lipolytic system of Acinetobacter calcoaceticus BD413: cloning of a gene encoding one of the esterases
    R G Kok
    Department of Microbiology, E C Slater Institute, BioCentrum Amsterdam, The Netherlands
    J Gen Microbiol 139:2329-42. 1993
    ..0 kDa. The amino acid sequence of this protein shows homology to a number of lipolytic enzymes, most notably to esterases. Deletion of estA only partially abolished cell-bound esterase activity in A...
  23. ncbi Paraoxonase active site required for protection against LDL oxidation involves its free sulfhydryl group and is different from that required for its arylesterase/paraoxonase activities: selective action of human paraoxonase allozymes Q and R
    M Aviram
    Lipid Research Laboratory, Technion Faculty of Medicine, The Rappaport Family Institute for Research in the Medical Sciences and Rambam Medical Center, Haifa, Israel
    Arterioscler Thromb Vasc Biol 18:1617-24. 1998
    ....
  24. ncbi C. elegans HIM-17 links chromatin modification and competence for initiation of meiotic recombination
    Kirthi C Reddy
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 118:439-52. 2004
    ..Furthermore, DSB levels and incidence of chiasmata can be modulated by loss of LIN-35/Rb. These and other data suggest that chromatin state governs the timing of DSB competence...
  25. ncbi Enzymes from extremophiles
    D C Demirjian
    ThermoGen, Inc, Chicago, IL 60612, USA
    Curr Opin Chem Biol 5:144-51. 2001
    ..This has also led to a greater understanding of stability factors involved in adaptation of these enzymes to their unusual environments...
  26. ncbi Plasma esterases and inflammation in ageing and frailty
    Ruth E Hubbard
    Department of Geriatric Medicine, School of Medicine, Cardiff University, 3rd Floor, Academic Centre, Llandough Hospital, Penarth, UK
    Eur J Clin Pharmacol 64:895-900. 2008
    b>Esterases are enzymes of drug metabolism known to be reduced in frail older people and during acute illness. The mechanism for this is unknown. The aim of this study was to examine esterase activity and inflammation in ageing and frailty.
  27. pmc Structural reorganization and preorganization in enzyme active sites: comparisons of experimental and theoretically ideal active site geometries in the multistep serine esterase reaction cycle
    Adam J T Smith
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    J Am Chem Soc 130:15361-73. 2008
    ..The results provide a useful guiding principle for de novo design of enzymes with multistep mechanisms...
  28. pmc Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from antarctic desert soil
    Caroline Heath
    Department of Biotechnology, University of the Western Cape, Cape Town, South Africa
    Appl Environ Microbiol 75:4657-9. 2009
    ..The enzyme was active over a temperature range of 7 to 54 degrees C and at alkaline pH levels and is a potential candidate for industrial application...
  29. ncbi Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics
    Xianchun Li
    Department of Entomology and BIO5, University of Arizona, Tucson, Arizona 85721, USA
    Annu Rev Entomol 52:231-53. 2007
    ..e., cytochrome P450 monooxygenases (P450s), esterases, and glutathione-S-transferases (GSTs), are the focus of this review...
  30. ncbi Paraoxonase 192 Gln-->Arg polymorphism: an independent risk factor for nonfatal arterial ischemic stroke among young adults
    Barbara Voetsch
    Whitaker Cardiovascular Institute, Evans Department of Medicine, Boston University School of Medicine, Boston, Mass 02118, USA
    Stroke 33:1459-64. 2002
    ..The association of these polymorphisms with the risk of AIS remains controversial...
  31. doi High-resolution structure of ybfF from Escherichia coli K12: a unique substrate-binding crevice generated by domain arrangement
    Suk Youl Park
    Department of Chemistry and Institute of Basic Sciences, Chonnam National University, 300 Yongbong Dong, Buk Gu, Gwangju 500 757, Korea
    J Mol Biol 376:1426-37. 2008
    b>Esterases are one of the most common enzymes and are involved in diverse cellular functions...
  32. ncbi Cold-active esterase from Psychrobacter sp. Ant300: gene cloning, characterization, and the effects of Gly-->Pro substitution near the active site on its catalytic activity and stability
    Ljudmila Kulakova
    Laboratory of Microbial Biochemistry, Institute for Chemical Research, Kyoto University, Uji, Kyoto fu, Kyoto 611 0011, Japan
    Biochim Biophys Acta 1696:59-65. 2004
    ....
  33. ncbi Relationship between age and plasma esterases
    K Abou-Hatab
    Department of Geriatric Medicine, Academic Centre, Llandough Hospital, Penarth, UK
    Age Ageing 30:41-5. 2001
    ..RESULTS: we found no correlation between the activities of any of the enzymes measured and advancing age. CONCLUSION: age per se is not associated with reductions in the activities of esterase enzymes...
  34. ncbi Functional expression and refolding of new alkaline esterase, EM2L8 from deep-sea sediment metagenome
    Hyo Jung Park
    Division of Biotechnology, The Catholic University of Korea, 43 1 Yokkok 2 dong, Wonmi gu, Bucheon, Gyeonggido 420 743, South Korea
    Protein Expr Purif 52:340-7. 2007
    ..Moreover, it was found to have an optimum pH of 10-11, thus revealing that it is an alkaline enzyme. In this paper, the new esterase EM2L8 buried in a deep-sea sediment became known on the surface and was characterized biochemically...
  35. doi A thermostable feruloyl-esterase from the hemicellulolytic bacterium Thermobacillus xylanilyticus releases phenolic acids from non-pretreated plant cell walls
    Harivony Rakotoarivonina
    UMR614 Fractionnement des AgroRessources et Environnement, INRA, 51686 Reims, France
    Appl Microbiol Biotechnol 90:541-52. 2011
    ..4 kDa, similar to feruloyl-esterases from cellulolytic bacteria and fungi...
  36. ncbi Characterization of digestive gland esterase-lipase activity of juvenile redclaw crayfish Cherax quadricarinatus
    Silverio López-López
    Laboratorio de Fisiología y Bioquímica Comparada, Centro de Investigaciones Biologicas del Noroeste, Mar Bermejo 195, Col Playa Palo Santa Rita, La Paz, BCS 23090, Mexico
    Comp Biochem Physiol B Biochem Mol Biol 135:337-47. 2003
    ..The enzyme activity diminished as the length of the fatty acid chain of substrates increased. Molecular masses for four isozymes were 43, 46, 63 and 118 kDa, respectively, as determined by PAGE...
  37. ncbi MRI cerebral white matter lesions and paraoxonase PON1 polymorphisms : three-year follow-up of the austrian stroke prevention study
    R Schmidt
    Department of Neurology, Karl Franzens University, Graz, Austria
    Arterioscler Thromb Vasc Biol 20:1811-6. 2000
    ..05/mm Hg), and LL paraoxonase genotype (odds ratio, 2. 65) to be significant predictors of WML progression. These data suggest that the LL PON1 genotype at position 54 influences the extent and progression of WMLs in elderly subjects...
  38. doi Two glucuronoyl esterases of Phanerochaete chrysosporium
    Miroslava Duranová
    Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dubravska cesta 9, 845 38, Bratislava, Slovakia
    Arch Microbiol 191:133-40. 2009
    ..Comparison of the amino acid sequences of identified and putative glucuronoyl esterases to other types of carbohydrate esterases (CE) confirmed that they form a separate family of CEs...
  39. ncbi Identification of two novel esterases from a marine metagenomic library derived from South China Sea
    Xinmin Chu
    Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230027, China
    Appl Microbiol Biotechnol 80:615-25. 2008
    ..were isolated from a marine microbial metagenomic library by functional screening, and the corresponding esterases EstA and EstB were biochemically characterized...
  40. ncbi Cloning, recombinant expression and biochemical characterisation of novel esterases from Bacillus sp. associated with the marine sponge Aplysina aerophoba
    A Karpushova
    Institut für Technische Biochemie, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    Appl Microbiol Biotechnol 67:59-69. 2005
    Two novel esterases (EstB1 and EstB2) were isolated from a genomic library of Bacillus sp. associated with the marine sponge Aplysina aerophoba...
  41. ncbi Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system
    Martin Ruppert
    Department of Pharmaceutical Biology, Institute of Pharmacy, Johannes Gutenberg University, Staudinger Weg 5, 55128, Mainz, Germany
    Planta 222:888-98. 2005
    ..This expression allowed molecular analysis of AAE for the first time and increased the number of functionally expressed alkaloid genes from Rauvolfia now to eight, and the number of ajmaline pathway-specific cDNAs to a total of six...
  42. doi Characterization and its potential application of two esterases derived from the arctic sediment metagenome
    Jeong Ho Jeon
    Marine Biotechnology Research Centre, Korean Ocean Research and Development Institute, Ansan, South Korea
    Mar Biotechnol (NY) 11:307-16. 2009
    ..The esterases genes were overexpressed in Escherichia coli by co-expressing GroEL-GroES chaperonine, and the recombinant ..
  43. ncbi Purification and cloning of an esterase from the weed black-grass (Alopecurus myosuroides), which bioactivates aryloxyphenoxypropionate herbicides
    Ian Cummins
    School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, UK
    Plant J 39:894-904. 2004
    ....
  44. ncbi Purification of ENOD8 proteins from Medicago sativa root nodules and their characterization as esterases
    David Pringle
    Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104, USA
    Plant Physiol Biochem 42:73-9. 2004
    ..Considering ENOD8 protein sequence similarity to an exopolygalacturonase/EP4/iEP4 and lanatoside 15'-O-acetylesterase with the results presented here predicts that ENOD8 substrates could be acetylated oligo- or polysaccharides...
  45. ncbi Isolation and characterization of the early nodule-specific protein homologue (Hev b 13), an allergenic lipolytic esterase from Hevea brasiliensis latex
    Siti Arija M Arif
    Rubber Research Institute of Malaysia, 50450 Kuala Lumpur, Malaysia
    J Biol Chem 279:23933-41. 2004
    ..The purified protein showed lipase and esterase activities and may be involved in plant defense...
  46. doi Identification and biochemical characterization of a GDSL-motif carboxylester hydrolase from Carica papaya latex
    Slim Abdelkafi
    CNRS, Aix Marseille Universite, Enzymologie Interfaciale et Physiologie de la Lipolyse, Marseille, France
    Biochim Biophys Acta 1791:1048-56. 2009
    ....
  47. ncbi Characterization of novel esterases in insecticide-resistant mosquitoes
    Feng Cui
    State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Insect Biochem Mol Biol 37:1131-7. 2007
    ..detected Ester alleles, Ester(B10) and Ester(11) (including associated Ester(A11) and Ester(B11)), coding for esterases B10 and A11-B11, respectively, are characterized qualitatively and quantitatively...
  48. ncbi Indication for a new lipolytic enzyme family: isolation and characterization of two esterases from a metagenomic library
    Sally Bayer
    Institute of Biochemistry, University of Leipzig, Germany
    J Mol Microbiol Biotechnol 18:181-7. 2010
    ..EstGK1 and EstZ3 have only minor overall sequence similarity to known esterases. We propose that, together with other hypothetical enzymes, they constitute a new family of lipolytic enzymes...
  49. ncbi Gene cloning and characterization of a novel thermophilic esterase from Fervidobacterium nodosum Rt17-B1
    Shanshan Yu
    Jilin University, Changchun, China
    Acta Biochim Biophys Sin (Shanghai) 42:288-95. 2010
    ..high with a half-life of 80 min at 70 degrees C, but less stable compared with some other hyperthermophilic esterases. A structural model was constructed using acetylesterase from Aspergillus aculeatus as a template...
  50. doi Production and characterization of two N-terminal truncated esterases from Thermus thermophilus HB27 in a mesophilic yeast: effect of N-terminus in thermal activity and stability
    Pablo Fuciños
    Department of Analytical and Food Chemistry, University of Vigo, Campus of Ourense, As Lagoas, 32004 Ourense, Spain
    Protein Expr Purif 78:120-30. 2011
    ..Minor differences were observed in the substrate specificity. Our results suggest that the N-terminal segment is critical for maintaining the hyperthermophilic function and stability...
  51. ncbi Characterization of two metagenome-derived esterases that reactivate chloramphenicol by counteracting chloramphenicol acetyltransferase
    Weixin Tao
    Department of Medical Bioscience, Dong A University, Busan 604 714, Korea
    J Microbiol Biotechnol 21:1203-10. 2011
    ..we investigated lipolytic enzymes selected from an alluvial soil metagenomic library, and identified two novel esterases, EstDL26 and EstDL136...
  52. ncbi Identification of human cytochrome P450 isoforms and esterases involved in the metabolism of mirabegron, a potent and selective β3-adrenoceptor agonist
    Shin Takusagawa
    Drug Metabolism Research Laboratories, Astellas Pharma Inc, Osaka, Japan
    Xenobiotica 42:957-67. 2012
    1. Human cytochrome P450 (CYP) enzymes and esterases involved in the metabolism of mirabegron, a potent and selective human β(3)-adrenoceptor agonist intended for the treatment of overactive bladder, were identified in in vitro studies...
  53. ncbi Biochemical and physiological studies of soluble esterases from Drosophila melanogaster
    M J Healy
    CSIRO Division of Entomology, Canberra, ACT, Australia
    Biochem Genet 29:365-88. 1991
    Twenty-two soluble esterases have been identified in D. melanogaster by combining the techniques of native polyacrylamide gel electrophoresis and isoelectric focusing...
  54. ncbi Esterases from Bacillus subtilis and B. stearothermophilus share high sequence homology but differ substantially in their properties
    E Henke
    Institute of Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstrasse 16, 17487 Greifswald, Germany
    Appl Microbiol Biotechnol 60:320-6. 2002
    ..Despite the high homology, both esterases revealed remarkable differences in their properties...
  55. doi Functional proteomic analysis of lipases and esterases in cultured human adipocytes
    Maximilian Schicher
    Institute of Biochemistry, Graz University of Technology, Graz, Austria
    J Proteome Res 9:6334-44. 2010
    ..Both of them showed pronounced hydrolytic activities on hydrophobic acylglycerols and therefore may be considered lipases. The physiological functions of the novel lipolytic proteins in vivo are currently subject to investigation...
  56. doi Overexpressed esterases in a fenvalerate resistant strain of the cotton bollworm, Helicoverpa armigera
    Shuwen Wu
    Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects Ministry of Agriculture, Nanjing 210095, China
    Insect Biochem Mol Biol 41:14-21. 2011
    ..with four putative carboxylesterase genes, all of which clustered within a phylogenetic clade of secreted midgut esterases. Quantitative PCR on these four genes showed several-fold greater expression in tissues of YGF compared to SCD ..
  57. ncbi A novel esterase from Bacillus subtilis (RRL 1789): purification and characterization of the enzyme
    Peerzada Kaiser
    Regional Research Laboratory, Canal Road, Jammu Tawi 180001, India
    Protein Expr Purif 45:262-8. 2006
    ..The N-terminal amino acid sequence of BSE determined, as Met-Thr-Pro-Glu-Iso-Val-Thr-Thr-Glu-Tyr-Gly- revealed similarity with the N-terminal amino acid sequence of p-nitrobenzylesterase of B. subtilis...
  58. ncbi Functions of the siderophore esterases IroD and IroE in iron-salmochelin utilization
    Mingang Zhu
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Microbiology 151:2363-72. 2005
    ..It is suggested that homologues in other bacteria are also periplasmic IroE-type esterases of siderophores.
  59. ncbi SPO11 is required for sex-body formation, and Spo11 heterozygosity rescues the prophase arrest of Atm-/- spermatocytes
    Marina A Bellani
    Genetics and Biochemistry Branch, NIDDK, NIH, Bethesda, MD 20892, USA
    J Cell Sci 118:3233-45. 2005
    ....
  60. doi Effects of four host plants on susceptibility of Spodoptera litura (Lepidoptera: Noctuidae) larvae to five insecticides and activities of detoxification esterases
    Ming Xue
    Department of Entomology, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, China
    Pest Manag Sci 66:1273-9. 2010
    ..The susceptibility of S. litura larvae reared on tobacco, Chinese cabbage, cowpea and sweet potato to phoxim, chlorfenapyr, methomyl, fenvalerate and emamectin benzoate under laboratory conditions was determined...
  61. ncbi The classification of esterases: an important gene family involved in insecticide resistance--a review
    Isabela Reis Montella
    Laboratório de Fisiologia e Controle de Artrópodes Vetores, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brazil
    Mem Inst Oswaldo Cruz 107:437-49. 2012
    ..Historically, insecticide resistance has driven research on insect esterases and schemes for their classification...
  62. doi The SGNH-hydrolase of Streptomyces coelicolor has (aryl)esterase and a true lipase activity
    Ana Bielen
    Laboratory of Molecular Genetics, Division of Molecular Biology, Ruder Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia
    Biochimie 91:390-400. 2009
    ..coelicolor SGNH-hydrolase generated three distinct groups consisting of proteins from Actinomycetales, Ascomycota and Nematoda. At present it seems that these enzymes are most conserved among soil inhabiting organisms...
  63. pmc Genetically-determined polymorphism of nonspecific esterases and phosphoglucomutase in eight introduced breeds of the silkworm, Bombyx mori, raised in Bulgaria
    Teodora Staykova
    Department of Developmental Biology, Section of Genetics, Faculty of Biology, University of Plovdiv, 4000 Plovdiv, Bulgaria
    J Insect Sci 8:18. 2008
    ..Polymorphism of nonspecific esterases from pupal haemolymph was analyzed, as well as of phosphoglucomutase from different organs of larvae, pupae and ..
  64. pmc The Lipase Engineering Database: a navigation and analysis tool for protein families
    Markus Fischer
    Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, D 70569 Stuttgart, Germany
    Nucleic Acids Res 31:319-21. 2003
    ..led.uni-stuttgart.de) integrates information on sequence, structure, and function of lipases, esterases, and related proteins...
  65. pmc An exocellular protein from the oil-degrading microbe Acinetobacter venetianus RAG-1 enhances the emulsifying activity of the polymeric bioemulsifier emulsan
    Horacio Bach
    Department of Molecular Microbiology and Biotechnology, George S Wise Faculty of Life Science, Tel Aviv University, Tel Aviv 69978, Israel
    Appl Environ Microbiol 69:2608-15. 2003
    ..Other proteins, including a His-tagged overexpressed esterase from the related organism Acinetobacter calcoaceticus BD4, showed no enhancement...
  66. ncbi Activity of lipases and esterases towards tertiary alcohols: insights into structure-function relationships
    Erik Henke
    Institute of Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstr 16, 17487 Greifswald, Germany
    Angew Chem Int Ed Engl 41:3211-3. 2002
  67. ncbi In vitro stability of methylmethacrylic acid, TEGDMA and HEMA exposed to esterases
    Mario Seiss
    Walther Straub Institute of Pharmacology and Toxicology, Ludwig Maximilians University of Munich, 80336 Munich, Germany
    Dent Mater 25:1044-9. 2009
    ....
  68. ncbi Risk of myocardial infarction associated with Gln/Arg 192 polymorphism in the human paraoxonase gene and diabetes mellitus. The REGICOR Investigators
    C Aubo
    Lipids and Cardiovascular Epidemiology Unit, Institut Municipal d Investigacio Medica, IMIM, Barcelona, Spain
    Eur Heart J 21:33-8. 2000
    ....
  69. ncbi Release of short chain fatty acids from cream lipids by commercial lipases and esterases
    K Saerens
    Laboratory of Industrial Microbiology and Biocatalysis, Faculty of Bioscience Engineering, Ghent University, Belgium
    Biotechnol Lett 30:311-5. 2008
    Lipases and esterases are frequently used in dairy production processes to enhance the buttery flavour of the end product...
  70. ncbi Cloning and expression in Escherichia coli of an esterase-coding gene from the oil-degrading bacterium Acinetobacter calcoaceticus RAG-1
    P G Reddy
    Department of Microbiology, George S Wise Faculty of Life Sciences, Tel Aviv University, Israel
    Gene 76:145-52. 1989
    ..The fact that the production of active esterase depended on the orientation of the est gene within the vector suggested that transcription proceeded from the tet promoter in pBR322...
  71. doi Characterization of a novel thermostable esterase from Thermus scotoductus SA-01: evidence of a new family of lipolytic esterases
    Erika M du Plessis
    CSIR Biosciences, Box 395, Pretoria, 0001, Gauteng, South Africa
    Curr Microbiol 60:248-53. 2010
    ..Sequence analysis showed that both proteins contained the G-X-S-X-G signature sequence motif present in most esterases and lipases...
  72. ncbi Production of flavour compounds from fat during cheese ripening by action of lipases and esterases
    Irma Verónica Wolf
    Instituto de Lactología Industrial, Facultad de Ingenieria Quimica, Universidad Nacional del Litoral CONICET Consejo Nacional de Investigaciones Científicas y Técnicas, Santiago del Estero 2829, S3000AOM, Santa Fe, Argentina
    Protein Pept Lett 16:1235-43. 2009
    ..Before starting with the specific study about formation of flavour compounds from milk fat during cheese ripening, a brief review of the technological aspects of cheese production is needed...
  73. ncbi Novel thermophilic and thermostable lipolytic enzymes from a Thailand hot spring metagenomic library
    Pacawadee Tirawongsaroj
    BIOTEC Central Research Unit, National Center for Genetic Engineering and Biotechnology, 113 National Science Park, Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand
    J Biotechnol 133:42-9. 2008
    ..The isolated enzymes, are therefore, different from other known patatin-like phospholipases and esterases, which usually show no activity for substrates longer than C(10)...
  74. ncbi Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism
    Charanjit Arora
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center and Weill Graduate School of Medical Sciences of Cornell University, 1275 York Avenue, Box 97, New York, NY 10021, USA
    Mol Cell 13:549-59. 2004
    ..Ski8 works with Spo11 to recruit other DSB proteins to meiotic chromosomes, implicating Ski8 as a scaffold protein mediating assembly of a multiprotein complex essential for DSB formation...
  75. ncbi Sex increases the efficacy of natural selection in experimental yeast populations
    Matthew R Goddard
    NERC Centre for Population Biology and Department of Biological Sciences, Imperial College London, Silwood Park Campus, Ascot SL5 7PY, UK
    Nature 434:636-40. 2005
    ..We show that, as predicted by the theory, sex increases the rate of adaptation to a new harsh environment but has no measurable effect on fitness in a new benign environment where there is little selection...
  76. pmc Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae
    Shohreh Maleki
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY 10021, USA
    Chromosoma 116:471-86. 2007
    ..These studies also suggest that an essential function of Rec102 and Rec104 is to connect Mei4 and Rec114 to Spo11...
  77. ncbi Differential gene expression of mammalian SPO11/TOP6A homologs during meiosis
    M Shannon
    Molecular and Structural Biology Division, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, CA 94550, USA
    FEBS Lett 462:329-34. 1999
    ..2-13.3, a region that is amplified in some breast cancers. Sequence homology and differential expression together support a highly conserved role for SPO11 in the enzymatic cleavage of DNA that accompanies meiotic recombination...
  78. ncbi Inter-individual variability in esterases in human liver
    Christopher Jewell
    Toxicology Unit, School of Clinical and Laboratory Sciences, 4th Floor, Devonshire Building, Devonshire Terrace, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4EA, UK
    Biochem Pharmacol 74:932-9. 2007
    ..Lower correlations with larger alcohol leaving group parabens are consistent with more hCE2 involvement...
  79. ncbi The role of esterases in the metabolism of ciclesonide to desisobutyryl-ciclesonide in human tissue
    Elaine Mutch
    Toxicology Unit, Clinical and Laboratory Sciences, The University of Newcastle, Newcastle upon Tyne, UK
    Biochem Pharmacol 73:1657-64. 2007
    Ciclesonide (CIC) is an inhaled glucocorticosteroid. This study aimed to identify esterases involved in the metabolism of CIC to the active metabolite desisobutyryl-ciclesonide (des-CIC), and to measure hydrolysis rates in human liver, ..
  80. ncbi Esterase 2-oligodeoxynucleotide conjugates as sensitive reporter for electrochemical detection of nucleic acid hybridization
    Yiran Wang
    Laboratorium fur Biochemie, Universitat Bayreuth, Universitätsstr 30, 95440 Bayreuth, Germany
    Biosens Bioelectron 22:1798-806. 2007
    ..Artificial single nucleotide mismatches in a 510-nucleotide ssDNA could be reliably determined using esterase 2-oligodeoxynucleotide conjugates as a reporter...
  81. ncbi Methods to increase enantioselectivity of lipases and esterases
    Uwe T Bornscheuer
    Institute of Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, Greifswald University, Soldmannstrasse 16, D 17487, Greifswald, Germany
    Curr Opin Biotechnol 13:543-7. 2002
    Lipases and esterases are frequently used in the synthesis of optically pure compounds; however, natural enzymes do not always show sufficiently high enantioselectivity...
  82. pmc Rapid degradation of oseltamivir phosphate in clinical samples by plasma esterases
    Niklas Lindegardh
    Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok 10400, Thailand
    Antimicrob Agents Chemother 50:3197-9. 2006
    The anti-influenza drug oseltamivir is an ester prodrug activated by hepatic carboxylesterases. Plasma esterases also convert up to 31...
  83. ncbi Chaperone-like activities of alpha-synuclein: alpha-synuclein assists enzyme activities of esterases
    Misun Ahn
    Division of Biotechnology and Molecular Engineering, College of Engineering, Ajou University, Suwon 443 749, Republic of Korea
    Biochem Biophys Res Commun 346:1142-9. 2006
    ..which has no well-defined secondary or tertiary structure, can protect the enzyme activity of microbial esterases against stress conditions such as heat, pH, and organic solvents...
  84. ncbi GDSL family of serine esterases/lipases
    Casimir C Akoh
    Department of Food Science and Technology, University of Georgia, Athens, GA 30602 7610, USA
    Prog Lipid Res 43:534-52. 2004
    GDSL esterases and lipases are hydrolytic enzymes with multifunctional properties such as broad substrate specificity and regiospecificity...
  85. ncbi Biochemical detection of pyrethroid resistance mechanisms in Anopheles minimus in Thailand
    Theeraphap Chareonviriyaphap
    Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
    J Vector Ecol 28:108-16. 2003
    ..Expression of levels of non-specific esterases, monooxygenases, and glutathione S-transferases (GSTs) were measured...
  86. pmc Isolation and biochemical characterization of two novel metagenome-derived esterases
    C Elend
    Molekulare Enzymtechnologie, Universitat Duisburg Essen, Lotharstrasse 1, 47057 Duisburg, Germany
    Appl Environ Microbiol 72:3637-45. 2006
    ..Both esterases are approximately 380 amino acids in size and show similarity to beta-lactamases, indicating that they belong to ..
  87. ncbi Role of the N terminus in enzyme activity, stability and specificity in thermophilic esterases belonging to the HSL family
    Luigi Mandrich
    Institute of Protein Biochemistry, CNR, Via P Castellino 111, 80131 Napoli, Italy
    J Mol Biol 345:501-12. 2005
    ..This is the first report highlighting the importance of the "cap" domain in the HSL family, since the N terminus partly contributes to the building up of this structure...
  88. ncbi The effect of piperonyl butoxide on pyrethroid-resistance-associated esterases in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)
    Susan J Young
    Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK
    Pest Manag Sci 61:397-401. 2005
    ..Biochemical studies have shown that pyrethroid-resistance-associated esterases in H armigera are inhibited by the insecticide synergist piperonyl butoxide (PBO)...
  89. pmc Distinct DNA-damage-dependent and -independent responses drive the loss of oocytes in recombination-defective mouse mutants
    Monica Di Giacomo
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center and Weill Graduate School of Medical Sciences of Cornell University, 1275 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:737-42. 2005
    ..The absence of ATM caused defects in folliculogenesis that were similar to those in Dmc1 mutants and that could be suppressed by Spo11 mutation, implying that oocyte death in Atm-deficient animals is a response to defective DSB repair...
  90. ncbi Comparative acaricide susceptibility and detoxifying enzyme activities in field-collected resistant and susceptible strains of Tetranychus urticae
    Thomas Van Leeuwen
    Laboratory of Agrozoology, Department of Crop Protection, Faculty of Agricultural and Applied Biological Science, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
    Pest Manag Sci 61:499-507. 2005
    ..Enhanced detoxification by increased activity of mono-oxygenases (MO) and esterases is at least partially responsible for the observed resistance and cross-resistance...
  91. pmc New thermophilic and thermostable esterase with sequence similarity to the hormone-sensitive lipase family, cloned from a metagenomic library
    Jin Kyu Rhee
    Department of Biotechnology, Yonsei University, 134 Sinchon dong, Seodaemun gu, Seoul 120 749, Korea
    Appl Environ Microbiol 71:817-25. 2005
    ..An amino acid sequence comparison with other esterases and lipases revealed that the enzyme should be classified as a new member of the hormone-sensitive lipase family...
  92. ncbi Roles played by esterase activity and by a sodium channel mutation involved in pyrethroid resistance in populations of Boophilus microplus (Acari: Ixodidae) collected from Yucatan, Mexico
    R Rosario-Cruz
    Centro Nacional de Investigaciones en Parasitología Veterinaria INIFAP, Morelos, Mexico
    J Med Entomol 42:1020-5. 2005
    ..It was not determined whether enhanced esterase-based hydrolytic activity was involved in pyrethroid resistance in the populations tested...
  93. ncbi Biochemical profiling in silico--predicting substrate specificities of large enzyme families
    Sadhna Tyagi
    Institute of Technical Biochemistry, University of Stuttgart, Germany
    J Biotechnol 124:108-16. 2006
    ..specificity of cholinesterases toward acetyl- and butyrylcholine, the changes of activity of lipases and esterases upon the size of the acid moieties, activity of lipases and esterases toward tertiary alcohols, and the ..
  94. ncbi Strategic selection of hyperthermophilic esterases for resolution of 2-arylpropionic esters
    Amitabh C Sehgal
    North Carolina State University, Department of Chemical Engineering, Raleigh, North Carolina 27695 7905, USA
    Biotechnol Prog 19:1410-6. 2003
    ....
  95. ncbi MELDB: a database for microbial esterases and lipases
    Ho Young Kang
    Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, KRIBB, P O Box 115, Yuseong, Daejeon 305 600, Republic of Korea
    FEBS Lett 580:2736-40. 2006
    MELDB is a comprehensive protein database of microbial esterases and lipases which are hydrolytic enzymes important in the modern industry...
  96. ncbi Detecting cellulase and esterase enzyme activities encoded by novel genes present in environmental DNA libraries
    Helen C Rees
    Department of Microbiology and Immunology, University of Leicester, University Road, Leicester, LE1 9HN, UK
    Extremophiles 7:415-21. 2003
    ..Esterase/lipase-positive clones from Lake Elmenteita were isolated with a frequency of 1 in 30,000 from a library made from an olive-oil enrichment as compared to 1 in 100,000 from an environmental library...
  97. ncbi Identification of a point mutation in an esterase gene in different populations of the southern cattle tick, Boophilus microplus
    R Hernandez
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station 77843 4467, USA
    Insect Biochem Mol Biol 30:969-77. 2000
    ..Identification of this mutation and the strong signal detected in southern blot with this strain, suggest that esterases are contributing to detoxification of pyrethroid compounds, as a resistant mechanism in Mexican strains of the ..
  98. ncbi Current progress on esterases: from molecular structure to function
    Tetsuo Satoh
    Biomedical Research Institute, Chiba University, Chiba, Japan
    Drug Metab Dispos 30:488-93. 2002
    ..This article covers mechanisms of regulation of gene expression of the various esterases by developmental factors and xenobiotics, as well as the interplay between physiological and chemical regulation ..
  99. ncbi The mouse Spo11 gene is required for meiotic chromosome synapsis
    P J Romanienko
    Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health 20892, Bethesda, MD, USA
    Mol Cell 6:975-87. 2000
    ..We speculate that there is an additional role for Spo11, after it generates DSBs, in synapsis...
  100. pmc Identification of residues in yeast Spo11p critical for meiotic DNA double-strand break formation
    Robert L Diaz
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Mol Cell Biol 22:1106-15. 2002
    ..These results are consistent with a multimeric structure for Spo11p in vivo but may also indicate that the amount of Spo11 protein is not a limiting factor for DSB formation in normal cells...
  101. pmc Screening, nucleotide sequence, and biochemical characterization of an esterase from Pseudomonas fluorescens with high activity towards lactones
    V Khalameyzer
    Institute for Technical Biochemistry, University of Stuttgart, 70569 Stuttgart, Germany
    Appl Environ Microbiol 65:477-82. 1999
    ..8 U/mg) to delta-valerolactone (36.5 U/mg). The activities towards the aliphatic esters were significantly lower; the only exception was the activity toward ethyl caprylate, which was the preferred substrate...

Research Grants70

  1. BASIS FOR CELLULAR SUSCEPTIBILITY TO BACTERIAL TOXINS
    CATHARINE SAELINGER; Fiscal Year: 1993
    ..injury will be obtained by immunohistochemical techniques and Western blot hybridization analysis for serine esterases, perforin, eosinophil cytotoxic proteins and autoantibodies...
  2. Molecular Mechanisms of Dietary Fat Digestion by Pancreatic Lipases
    Mark E Lowe; Fiscal Year: 2010
    ..Lipases differ from esterases that act on water soluble substrates in that lipases must absorb to the interface of the substrate emulsion ..
  3. Molecular Mechanisms of Dietary Fat Digestion by Pancreatic Lipases
    Mark Lowe; Fiscal Year: 2009
    ..Lipases differ from esterases that act on water soluble substrates in that lipases must absorb to the interface of the substrate emulsion ..
  4. Novel mechanisms by which aspirin might protect against atherosclerosis
    Sampath Parthasarathy; Fiscal Year: 2010
    ..Hydrolysis was competed for by substrates of "aryl esterases" that prompted us to propose that paraoxonase 1 (PON 1) type of enzymes might be involved in the metabolism of ..
  5. Novel mechanisms by which aspirin might protect against atherosclerosis
    Sampath Parthasarathy; Fiscal Year: 2009
    ..Hydrolysis was competed for by substrates of "aryl esterases" that prompted us to propose that paraoxonase 1 (PON 1) type of enzymes might be involved in the metabolism of ..
  6. Improving CPT-11 Efficacy Using Structural Biology
    MATTHEW REDINBO; Fiscal Year: 2007
    CPT-11 is a camptothecin prodrug that is activated by esterases to yield SN-38, a potent topoisomerase I poison...
  7. BIOSYNTHESIS AND REGULATION OF SUBSTITUTED SIALIC ACIDS
    Ajit Varki; Fiscal Year: 1990
    ..particularly in retinoblastoma cells that are deficient in it, and to search for other sialic-acid-apecific esterases that could be involved in regulating O-acetylation...
  8. PHOSPHOLIPID TRANSFER DURING INTESTINAL ABSORPTION
    John Nichols; Fiscal Year: 1992
    ..mucosal brush border membranes, where is it produced, and is it structurally related to other known lipases and esterases? Phospholipids and lysophospholipids labeled with the fluorescent probe NBD (7-nitro-2,1,3-benzoxadiazol-4-yl) ..
  9. MODE OF ACTION AND METABOLISM OF ORGANIC TOXICANTS
    JOHN CASIDA; Fiscal Year: 1993
    ..mechanisms and implications of sequential phosphorylation and alkylation reactions within the catalytic site of esterases important in delayed neurotoxicity and other long-term toxicological lesions...
  10. MOLECULAR STRUCTURE OF STEROIDS
    William Duax; Fiscal Year: 1999
    ..A comparison of the structure of Cc ChE with those of other esterases should reveal the basis for cholesterol specificity and permit design of specific inhibitors of ChE that would ..
  11. MOLECULAR STRUCTURE OF STEROIDS
    William Duax; Fiscal Year: 2001
    ..A comparison of the structure of Cc ChE with those of other esterases should reveal the basis for cholesterol specificity and permit design of specific inhibitors of ChE that would ..
  12. MOLECULAR STRUCTURE OF STEROIDS
    William Duax; Fiscal Year: 2000
    ..A comparison of the structure of Cc ChE with those of other esterases should reveal the basis for cholesterol specificity and permit design of specific inhibitors of ChE that would ..
  13. Development Of An Eye Drop To Treat Presbyopia
    William H Garner; Fiscal Year: 2012
    ..The co-drug elicits no acute toxicology in Draize testing in rabbits. The co-drug is cleaved by cellular esterases to release a pro-reductant, that when converted to active reducing agent by cellular oxidoreductases, increases ..
  14. HYDROLYTIC ENZYMES IN THE METABOLISM OF TOXINS
    Bruce Hammock; Fiscal Year: 2000
    ..This proposal targets two enzyme classes, epoxide hydrolases (EHs) and esterases, which are both members of the alpha/beta-hydrolase fold family...
  15. HYDROLYTIC ENZYMES IN THE METABOLISM OF TOXINS
    Bruce Hammock; Fiscal Year: 1999
    ..This proposal targets two enzyme classes, epoxide hydrolases (EHs) and esterases, which are both members of the alpha/beta-hydrolase fold family...
  16. HYDROLYTIC ENZYMES IN THE METABOLISM OF TOXINS
    Bruce Hammock; Fiscal Year: 2004
    ..This proposal targets two enzyme classes, epoxide hydrolases (EHs) and esterases, which are both members of the alpha/beta-hydrolase fold family...
  17. HYDROLYTIC ENZYMES IN THE METABOLISM OF TOXINS
    Bruce Hammock; Fiscal Year: 2007
    ..This proposal targets two enzyme classes, epoxide hydrolases (EHs) and esterases, which are both members of the alpha/beta-hydrolase fold family...
  18. HYDROLYTIC ENZYMES IN THE METABOLISM OF TOXINS
    Bruce Hammock; Fiscal Year: 2001
    ..This proposal targets two enzyme classes, epoxide hydrolases (EHs) and esterases, which are both members of the alpha/beta-hydrolase fold family...
  19. HYDROLYTIC ENZYMES IN THE METABOLISM OF TOXINS
    Bruce Hammock; Fiscal Year: 2002
    ..This proposal targets two enzyme classes, epoxide hydrolases (EHs) and esterases, which are both members of the alpha/beta-hydrolase fold family...
  20. HYDROLYTIC ENZYMES IN THE METABOLISM OF TOXINS
    Bruce Hammock; Fiscal Year: 2003
    ..This proposal targets two enzyme classes, epoxide hydrolases (EHs) and esterases, which are both members of the alpha/beta-hydrolase fold family...
  21. HYDROLYTIC ENZYMES IN THE METABOLISM OF TOXINS
    Bruce Hammock; Fiscal Year: 2006
    ..This proposal targets two enzyme classes, epoxide hydrolases (EHs) and esterases, which are both members of the alpha/beta-hydrolase fold family...
  22. HYDROLYTIC ENZYMES IN THE METABOLISM OF TOXINS
    Bruce Hammock; Fiscal Year: 2005
    ..This proposal targets two enzyme classes, epoxide hydrolases (EHs) and esterases, which are both members of the alpha/beta-hydrolase fold family...
  23. STEROID HORMONES IN BREAST CANCER
    RICHARD HOCHBERG; Fiscal Year: 1992
    ..They require hydrolytic activation by esterases. We have found that LE2 is synthesized and sequestered in hydrophobic tissues, such as fat, including the fat of ..
  24. Control of Urban and Peri-urban Culex Mosquitoes
    Gregory Lanzaro; Fiscal Year: 2006
    ..this by developing novel pyrethoid-like fluorescent substrates for detection of P450 mixed function oxidases and esterases in Culex mosquitoes. These new substrates are highly sensitive...
  25. Control of Urban and Peri-urban Culex Mosquitoes
    Gregory Lanzaro; Fiscal Year: 2003
    ..this by developing novel pyrethoid-like fluorescent substrates for detection of P450 mixed function oxidases and esterases in Culex mosquitoes. These new substrates are highly sensitive...
  26. Control of Urban and Peri-urban Culex Mosquitoes
    Gregory Lanzaro; Fiscal Year: 2005
    ..this by developing novel pyrethoid-like fluorescent substrates for detection of P450 mixed function oxidases and esterases in Culex mosquitoes. These new substrates are highly sensitive...
  27. Control of Urban and Peri-urban Culex Mosquitoes
    Gregory Lanzaro; Fiscal Year: 2004
    ..this by developing novel pyrethoid-like fluorescent substrates for detection of P450 mixed function oxidases and esterases in Culex mosquitoes. These new substrates are highly sensitive...
  28. Control of Urban and Peri-urban Culex Mosquitoes
    Gregory Lanzaro; Fiscal Year: 2007
    ..this by developing novel pyrethoid-like fluorescent substrates for detection of P450 mixed function oxidases and esterases in Culex mosquitoes. These new substrates are highly sensitive...
  29. MEDIATORS OF INFLAMMATION IN THE HUMAN LUNG
    HAROLD NEWBALL; Fiscal Year: 1980
    ..The other two proteins are both arginine esterases, one generates a kinin from low molecular weight kininogen, while the other activates prekallikrein...
  30. STEROID METABOLISM AND ACTION IN CANCER
    Mortimer Levitz; Fiscal Year: 1993
    ..the proposal would exploit the MCF-7 breast cancer cell line as a model to test the role of estrogen- sensitive esterases in the control of cell proliferation...
  31. MEIBOMIAN KERATOCONJUNCTIVITIS
    James McCulley; Fiscal Year: 1999
    ..In addition, isolated bacteria have been tested for production of specific exoenzymes such as lipases and esterases which could produce secondary lipid changes...
  32. Metabolism and Age-Related Organophosphate Toxicity
    Janice Chambers; Fiscal Year: 2005
    ..levels in both adults and juveniles are strongly related to the levels and the efficiency of several protective esterases. We plan to test our hypothesis by investigating the following two specific aims: 1...
  33. Metabolism and Age-Related Organophosphate Toxicity
    Janice Chambers; Fiscal Year: 2004
    ..levels in both adults and juveniles are strongly related to the levels and the efficiency of several protective esterases. We plan to test our hypothesis by investigating the following two specific aims: 1...
  34. Metabolism and Age-Related Organophosphate Toxicity
    Janice Chambers; Fiscal Year: 2003
    ..levels in both adults and juveniles are strongly related to the levels and the efficiency of several protective esterases. We plan to test our hypothesis by investigating the following two specific aims: 1...
  35. Metabolism and Age-Related Organophosphate Toxicity
    Janice Chambers; Fiscal Year: 2002
    ..levels in both adults and juveniles are strongly related to the levels and the efficiency of several protective esterases. We plan to test our hypothesis by investigating the following two specific aims: 1...
  36. STEROID HORMONES IN BREAST CANCER
    RICHARD HOCHBERG; Fiscal Year: 1990
    ..ascertain whether these circulating compounds are estrogenic messengers that are specific for tissues containing esterases capable of releasing estradiol...
  37. MEIBOMIAN KERATOCONJUNCITIVITIS
    James McCulley; Fiscal Year: 1991
    ..In addition, isolated bacteria are being tested for production of specific exoenzymes such as lipases and esterases; other activity such as formation of cyclopropanes from unsaturated fatty acids is also being evaluated...
  38. Tissue Kallikrein In Immune Mediated Nephritis
    Quan Zhen Li; Fiscal Year: 2009
    ..Klks constitute a multi-gene family of serine esterases with a wide spectrum of biological functions...
  39. Tissue Kallikrein In Immune Mediated Nephritis
    Quan Zhen Li; Fiscal Year: 2010
    ..Klks constitute a multi-gene family of serine esterases with a wide spectrum of biological functions...
  40. CELLULAR MECHANISMS IN OVARIAN LIPID METABOLISM
    LAURIE PAAVOLA; Fiscal Year: 1990
    ..cholesteryl eshers, we will determine if HDL-cholesteryl esters are hydrolyzed by extracellular or intracellular esterases. Agents disrupting microtubules will aid in determining if the cytoskeleton is involved in transporting LDL-..
  41. Improving Absorption and Targeting of Antiviral Drugs
    John Hilfinger; Fiscal Year: 2009
    ..designing prodrugs targeted to transporters expressed in human intestine and able to be activated by endogenous esterases or amidases that release the absorbed drug in vivo from its prodrug form...
  42. Improving Absorption and Targeting of Antiviral Drugs
    John Hilfinger; Fiscal Year: 2007
    ..designing prodrugs targeted to transporters expressed in human intestine and able to be activated by endogenous esterases or amidases that release the absorbed drug in vivo from its prodrug form...
  43. Molecular and Cellular Interactions in Atherosclerosis
    Aldons Lusis; Fiscal Year: 2003
    ..PON1 and PON3 are members of a family of esterases, and both are carried on HDL...
  44. ROLE OF SPHINGOMYELIN IN LIPOPROTEIN METABOLISM
    Papasani Subbaiah; Fiscal Year: 2009
    ..up- or down regulated by ablation of ABCG1? There could be changes in cellular cholesterol content, cholesterol esterases, ACAT, ABCA1, or possibly SR-B1 that could affect efflux...
  45. ROLE OF SPHINGOMYELIN IN LIPOPROTEIN METABOLISM
    Papasani V Subbaiah; Fiscal Year: 2010
    ..up- or down regulated by ablation of ABCG1? There could be changes in cellular cholesterol content, cholesterol esterases, ACAT, ABCA1, or possibly SR-B1 that could affect efflux...
  46. BIOLOGICALLY ACTIVE STEROID ANALOGS
    RICHARD HOCHBERG; Fiscal Year: 2000
    ..Since they are readily cleaved by esterases into the charged carboxylic compounds which are not estrogenic they are very labile and do not induce ..
  47. BIOLOGICALLY ACTIVE STEROID ANALOGS
    RICHARD HOCHBERG; Fiscal Year: 1999
    ..Since they are readily cleaved by esterases into the charged carboxylic compounds which are not estrogenic they are very labile and do not induce ..
  48. BLOOD-BRAIN BARRIER TRANSPORT OF THYMIDINE ANALOGS
    Ashim Mitra; Fiscal Year: 1990
    ..5'-ester derivatives have been selected for their wide range of lipophilicities and structural specificities for esterases. The latentiated analogues will undergo appreciable transport (compared to IDU itself) across the blood-brain ..
  49. Perfluorocarbon Materials As Drug Delivery Vehicles
    Hans Joachim Lehmler; Fiscal Year: 2003
    ..These prodrugs show a high solubility in PFOB and are also water soluble, readily hydrolyzed in the presence of esterases and show little cytotoxicity...
  50. Perfluorocarbon Materials As Drug Delivery Vehicles
    Hans Joachim Lehmler; Fiscal Year: 2004
    ..These prodrugs show a high solubility in PFOB and are also water soluble, readily hydrolyzed in the presence of esterases and show little cytotoxicity...
  51. EICOSANOID SYNTHESIS
    John Falck; Fiscal Year: 1990
    ..Further metabolism of these producet by cytochrome P-450, glutathione transferases, esterases, hydroleses, and other enzyme systems has been documented...
  52. EICOSANOID SYNTHESIS
    John Falck; Fiscal Year: 1991
    ..Further metabolism of these producet by cytochrome P-450, glutathione transferases, esterases, hydroleses, and other enzyme systems has been documented...
  53. HUMAN SERUM PARAOXONASE/ARYLESTERASE
    Bert La Du; Fiscal Year: 1992
    ..center amino acid components, physical properties, substrate specificity, and its structural homology with other esterases having cysteine in their active centers...
  54. GROWTH AND NUTRITION IN ALAGILLE SYNDROME
    ANDREW MULBERG; Fiscal Year: 1999
    ..on not only the solubilization by bile acids into mixed micelles, but also requires pancreatic and intestinal esterases for hydrolysis before traversing the aqueous water layer in the intestinal lumen into the enterocyte...
  55. GROWTH AND NUTRITION IN ALAGILLE SYNDROME
    ANDREW MULBERG; Fiscal Year: 1999
    ..on not only the solubilization by bile acids into mixed micelles, but also requires pancreatic and intestinal esterases for hydrolysis before traversing the aqueous water layer in the intestinal lumen into the enterocyte...
  56. ORAL MUCOSAL CELL RESPONSES TO DENTAL RESIN COMPONENTS
    George Schuster; Fiscal Year: 1999
    ..The ability of cells and tissue esterases to hydrolyze the materials will also be assessed using cell homogenates and commercial tissue esterases...
  57. STRUCTURAL CHANGES IN HEMOGLOBIN
    Lynn Ten Eyck; Fiscal Year: 1999
    ..Further calculations are planned to estimate the transition states of such OP reactions with active serine of AchE and other esterases.
  58. STRUCTURAL CHANGES IN HEMOGLOBIN
    Lynn Ten Eyck; Fiscal Year: 2000
    ..Further calculations are planned to estimate the transition states of such OP reactions with active serine of AchE and other esterases.
  59. STRUCTURAL CHANGES IN HEMOGLOBIN
    Lynn Ten Eyck; Fiscal Year: 2001
    ..Further calculations are planned to estimate the transition states of such OP reactions with active serine of AchE and other esterases.
  60. Improving CPT-11 Efficacy Using Structural Biology
    MATTHEW REDINBO; Fiscal Year: 2006
    DESCRIPTION (provided by applicant): CPT-11 is a camptothecin prodrug that is activated by esterases to yield SN-38, a potent topoisomerase I poison...