urease

Summary

Summary: An enzyme that catalyzes the conversion of urea and water to carbon dioxide and ammonia. EC 3.5.1.5.

Top Publications

  1. pmc Real-time imaging of trapping and urease-dependent transmigration of Cryptococcus neoformans in mouse brain
    Meiqing Shi
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    J Clin Invest 120:1683-93. 2010
  2. ncbi Oral immunization with urease and Escherichia coli heat-labile enterotoxin is safe and immunogenic in Helicobacter pylori-infected adults
    P Michetti
    Division of Gastroenterology, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland
    Gastroenterology 116:804-12. 1999
  3. pmc Urease as a virulence factor in experimental cryptococcosis
    G M Cox
    Division of Infectious Disease, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Infect Immun 68:443-8. 2000
  4. pmc Mycobacterium bovis BCG urease attenuates major histocompatibility complex class II trafficking to the macrophage cell surface
    Khalid Sendide
    Division of Infectious Diseases, Department of Medicine, University of British Columbia, Rm 452D, 2733 Heather Street, Vancouver, BC, Canada V5Z 3J5
    Infect Immun 72:4200-9. 2004
  5. ncbi Comparison between magnifying endoscopy and histological, culture and urease test findings from the gastric mucosa of the corpus
    K Yagi
    Department of Internal Medicine, Niigata Prefectural Yoshida Hospital, Japan
    Endoscopy 34:376-81. 2002
  6. pmc The novel Helicobacter pylori CznABC metal efflux pump is required for cadmium, zinc, and nickel resistance, urease modulation, and gastric colonization
    Frank Nils Stähler
    Department of Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University Hospital Freiburg, Hermann Herder Str 11, D 79104 Freiburg, Germany
    Infect Immun 74:3845-52. 2006
  7. ncbi Nitrate reductase, arginine deaminase, urease and dehydrogenase activities in natural soil (ridges with forest) and in cotton soil after acetamiprid treatments
    Dileep K Singh
    Pesticide Toxicology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007, India
    Chemosphere 71:412-8. 2008
  8. doi In vivo interactome of Helicobacter pylori urease revealed by tandem affinity purification
    Kerstin Stingl
    Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, 75015 Paris, France
    Mol Cell Proteomics 7:2429-41. 2008
  9. pmc Urease activity represents an alternative pathway for Mycobacterium tuberculosis nitrogen metabolism
    Wenwei Lin
    Department of Microbiology, Immunology Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    Infect Immun 80:2771-9. 2012
  10. pmc Iron-containing urease in a pathogenic bacterium
    Eric L Carter
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA
    Proc Natl Acad Sci U S A 108:13095-9. 2011

Detail Information

Publications355 found, 100 shown here

  1. pmc Real-time imaging of trapping and urease-dependent transmigration of Cryptococcus neoformans in mouse brain
    Meiqing Shi
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    J Clin Invest 120:1683-93. 2010
    ..Using a knockout strain of C. neoformans, we demonstrated that transmigration into the mouse brain is urease dependent. To determine whether this could be amenable to therapy, we used the urease inhibitor flurofamide...
  2. ncbi Oral immunization with urease and Escherichia coli heat-labile enterotoxin is safe and immunogenic in Helicobacter pylori-infected adults
    P Michetti
    Division of Gastroenterology, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland
    Gastroenterology 116:804-12. 1999
    Oral immunization with Helicobacter pylori urease can cure Helicobacter infection in animals. As a step toward therapeutic immunization in humans, the safety and immunogenicity of oral immunization with recombinant H...
  3. pmc Urease as a virulence factor in experimental cryptococcosis
    G M Cox
    Division of Infectious Disease, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Infect Immun 68:443-8. 2000
    b>Urease catalyzes the hydrolysis of urea to ammonia and carbamate and has been found to be an important pathogenic factor for certain bacteria...
  4. pmc Mycobacterium bovis BCG urease attenuates major histocompatibility complex class II trafficking to the macrophage cell surface
    Khalid Sendide
    Division of Infectious Diseases, Department of Medicine, University of British Columbia, Rm 452D, 2733 Heather Street, Vancouver, BC, Canada V5Z 3J5
    Infect Immun 72:4200-9. 2004
    ..In this study we examined whether intracellular alkalinization due to mycobacterial urease could account for the defect in intracellular trafficking of class II molecules...
  5. ncbi Comparison between magnifying endoscopy and histological, culture and urease test findings from the gastric mucosa of the corpus
    K Yagi
    Department of Internal Medicine, Niigata Prefectural Yoshida Hospital, Japan
    Endoscopy 34:376-81. 2002
    ..pylori-negative normal stomach were described. This report describes the specific histological features and magnified views of the H. pylori-negative stomach and compares them with those seen during H. pylori-induced gastritis...
  6. pmc The novel Helicobacter pylori CznABC metal efflux pump is required for cadmium, zinc, and nickel resistance, urease modulation, and gastric colonization
    Frank Nils Stähler
    Department of Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University Hospital Freiburg, Hermann Herder Str 11, D 79104 Freiburg, Germany
    Infect Immun 74:3845-52. 2006
    ..pylori metal resistance determinant, which is required for gastric colonization and for the modulation of urease activity. Insertional mutagenesis of the HP0971, HP0970, or HP0969 genes in H...
  7. ncbi Nitrate reductase, arginine deaminase, urease and dehydrogenase activities in natural soil (ridges with forest) and in cotton soil after acetamiprid treatments
    Dileep K Singh
    Pesticide Toxicology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007, India
    Chemosphere 71:412-8. 2008
    ..Nitrate reductase, arginine deaminase, urease and dehydrogenase activities were high in natural soil in comparison to control soil and insecticide treated soil ..
  8. doi In vivo interactome of Helicobacter pylori urease revealed by tandem affinity purification
    Kerstin Stingl
    Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, 75015 Paris, France
    Mol Cell Proteomics 7:2429-41. 2008
    In the human gastric bacterium Helicobacter pylori, two metalloenzymes, hydrogenase and urease, are essential for in vivo colonization, the latter being a major virulence factor...
  9. pmc Urease activity represents an alternative pathway for Mycobacterium tuberculosis nitrogen metabolism
    Wenwei Lin
    Department of Microbiology, Immunology Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    Infect Immun 80:2771-9. 2012
    b>Urease represents a critical virulence factor for some bacterial species through its alkalizing effect, which helps neutralize the acidic microenvironment of the pathogen...
  10. pmc Iron-containing urease in a pathogenic bacterium
    Eric L Carter
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA
    Proc Natl Acad Sci U S A 108:13095-9. 2011
    Helicobacter mustelae, a gastric pathogen of ferrets, synthesizes a distinct iron-dependent urease in addition to its archetypical nickel-containing enzyme...
  11. pmc Dependence of Helicobacter pylori urease activity on the nickel-sequestering ability of the UreE accessory protein
    Stephane Benoit
    Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA
    J Bacteriol 185:4787-95. 2003
    The Helicobacter pylori ureE gene product was previously shown to be required for urease expression, but its characteristics and role have not been determined...
  12. doi Growth-inhibiting, bactericidal, and urease inhibitory effects of Paeonia lactiflora root constituents and related compounds on antibiotic-susceptible and -resistant strains of Helicobacter pylori
    Luong Thi My Ngan
    Interdisciplinary Program in Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151 921, Republic of Korea
    J Agric Food Chem 60:9062-73. 2012
    An assessment was made of the growth-inhibiting, bactericidal, and urease inhibitory activities of paeonol (PA), benzoic acid (BA), methyl gallate (MG), and 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranose (PGG) identified in Paeonia ..
  13. ncbi Staying alive overdosed: how does Helicobacter pylori control urease activity?
    Kerstin Stingl
    Institut Pasteur, Unité de Pathogénie Bactérienne des Muqueuses, 28, rue du Dr Roux, F 75724 Paris, France
    Int J Med Microbiol 295:307-15. 2005
    b>Urease, a nickel metalloenzyme is an essential virulence factor of the gastric pathogen, Helicobacter pylori...
  14. ncbi 13C-urea breath test to assess Helicobacter pylori bacterial load
    Rocco Maurizio Zagari
    Department of Internal Medicine and Gastroenterology, University of Bologna, Italy
    Helicobacter 10:615-9. 2005
    ..Our aim is to evaluate the correlation between 13C-UBT values and H. pylori bacterial load...
  15. doi Ureases as a target for the treatment of gastric and urinary infections
    C Follmer
    Department of Physical Chemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941 909, Brazil
    J Clin Pathol 63:424-30. 2010
    b>Urease is known to be a major contributor to pathologies induced by Helicobacter pylori and Proteus species...
  16. ncbi Cutting edge: urease release by Helicobacter pylori stimulates macrophage inducible nitric oxide synthase
    Alain P Gobert
    Department of Medicine, Division of Gastroenterology, and Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Immunol 168:6002-6. 2002
    ..iNOS by coculturing macrophages with wild-type or mutant strains deficient in VacA, CagA, picB product, or urease (ureA(-)). When filters were used to separate H...
  17. pmc Interplay of metal ions and urease
    Eric L Carter
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824 4320, USA
    Metallomics 1:207-21. 2009
    b>Urease, the first enzyme to be crystallized, contains a dinuclear nickel metallocenter that catalyzes the decomposition of urea to produce ammonia, a reaction of great agricultural and medical importance...
  18. pmc Urease produced by Coccidioides posadasii contributes to the virulence of this respiratory pathogen
    Fariba Mirbod-Donovan
    Department of Biology, Margaret Batts Tobin Building, Room 1 308E, University of Texas at San Antonio, 6900 North Loop 1604 West, San Antonio, TX 78249, USA
    Infect Immun 74:504-15. 2006
    b>Urease activity during in vitro growth in the saprobic and parasitic phases of Coccidioides spp...
  19. pmc Unprecedented loss of ammonia assimilation capability in a urease-encoding bacterial mutualist
    Laura E Williams
    The Institute for Genome Sciences and Policy, Duke University, Durham, NC, USA
    BMC Genomics 11:687. 2010
    ..Blochmannia perform key nutritional functions for the host, including synthesis of several essential amino acids. We used Illumina technology to sequence the genome of Blochmannia associated with Camponotus vafer...
  20. ncbi Insights into the role and structure of plant ureases
    Cristian Follmer
    Departamento de Fisico Quimica, Instituto de Quimica, Universidade Federal do Rio de Janeiro, CT, Bloco A S410, Rio de Janeiro 21941 909, Brazil
    Phytochemistry 69:18-28. 2008
    ..Since the isolation and characterization of jack bean urease by Sumner in 1926, many investigations have been dedicated to the structural and biological features of this ..
  21. pmc Safety and efficacy of low dose Escherichia coli enterotoxin adjuvant for urease based oral immunisation against Helicobacter pylori in healthy volunteers
    S Banerjee
    Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA
    Gut 51:634-40. 2002
    ..This study was undertaken to establish a safe and effective dose of LT, to confirm the safety of recombinant urease, and to compare the immunogenicity of orally compared with enterically delivered urease.
  22. ncbi Interactions among the seven Helicobacter pylori proteins encoded by the urease gene cluster
    Petra Voland
    Department of Medicine II, Technical University, 81675 Munich, Germany
    Am J Physiol Gastrointest Liver Physiol 284:G96-G106. 2003
    Survival of Helicobacter pylori in acid depends on intrabacterial urease. This urease is a Ni(2+)-containing oligomeric heterodimer. Regulation of its activity and assembly is important for gastric habitation by this neutralophile...
  23. pmc Distribution of the urease gene cluster among and urease activities of enterohemorrhagic Escherichia coli O157 isolates from humans
    Alexander W Friedrich
    Institut fur Hygiene, Universitatsklinikum Munster, Robert Koch Str 41, 48149 Munster, Germany
    J Clin Microbiol 43:546-50. 2005
    ..Here we studied the conservation of urease genes and their expression in sorbitol-fermenting (SF) and non-SF EHEC O157 isolates...
  24. ncbi Jack bean urease: the effect of active-site binding inhibitors on the reactivity of enzyme thiol groups
    Barbara Krajewska
    Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30 060 Krakow, Poland
    Bioorg Chem 35:355-65. 2007
    In view of the complexity of the role of the active site flap cysteine in the urease catalysis, in this work we studied how the presence of typical active-site binding inhibitors of urease, phenylphosphorodiamidate (PPD), acetohydroxamic ..
  25. ncbi Urease of Corynebacterium glutamicum: organization of corresponding genes and investigation of activity
    L Nolden
    Institut fur Biochemie, Universität zu Kon, Cologne, Germany
    FEMS Microbiol Lett 189:305-10. 2000
    The Corynebacterium glutamicum genes encoding urease were isolated and sequenced. While ureA, ureB and ureC are encoding structural subunits of urease, ureE, ureF, ureG and ureD are encoding accessory proteins...
  26. pmc Mimotopes selected with a neutralizing antibody against urease B from Helicobacter pylori induce enzyme inhibitory antibodies in mice upon vaccination
    Yan Li
    School of Biotechnology, Southern Medical University, Guangzhou Dadaobei No, 1838, Guangzhou, China
    BMC Biotechnol 10:84. 2010
    b>Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa...
  27. pmc Urease expression by Cryptococcus neoformans promotes microvascular sequestration, thereby enhancing central nervous system invasion
    Michal A Olszewski
    Veteran s Administration Medical Center Ann Arbor, Ann Arbor, Michigan, USA
    Am J Pathol 164:1761-71. 2004
    Our objective was to determine the role of the cryptococcal virulence factor urease in pulmonary-to-central nervous system, dissemination, invasion, and growth. C...
  28. pmc In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity
    Kylie J Nolan
    School of Microbiology and Immunology, The University of New South Wales, Australia
    Infect Immun 70:685-91. 2002
    Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined...
  29. doi Structure of the UreD-UreF-UreG-UreE complex in Helicobacter pylori: a model study
    Francesco Biagi
    Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy
    J Biol Inorg Chem 18:571-7. 2013
    The molecular details of the protein complex formed by UreD, UreF, UreG, and UreE, accessory proteins for urease activation in the carcinogenic bacterium Helicobacter pylori, have been elucidated using computational modeling...
  30. ncbi Isolation of urease-positive Ochrobactrum intermedium in the stomach of a non-ulcer dyspeptic patient from north India
    Mahesh S Dharne
    Molecular Biology Unit, National Centre for Cell Science, Ganeshkhind Road, Pune University Campus, Pune, Maharashtra, India
    J Microbiol Immunol Infect 41:183-6. 2008
    ..intermedium LMG3301. Further investigation is required in order to evaluate the link between H. pylori and O. intermedium in the gastric niche...
  31. pmc Helicobacter typhlonius sp. nov., a Novel Murine Urease-Negative Helicobacter Species
    C L Franklin
    Research Animal Diagnostic and Investigative Laboratory, Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri 65211, USA
    J Clin Microbiol 39:3920-6. 2001
    ..In this report, we describe a novel urease-negative helicobacter, Helicobacter typhlonius sp. nov...
  32. ncbi Medium pH-dependent redistribution of the urease of Helicobacter pylori
    Wu Hong
    Department of Microbiology, Osaka Medical College, 2 7 Daigaku machi, Takatsuki shi, Osaka 569 8686, Japan
    J Med Microbiol 52:211-6. 2003
    Helicobacter pylori is an aetiological agent of gastric disease. Although the role of urease in gastric colonization of H. pylori has been shown, it remains unclear as to where urease is located in this bacterial cell...
  33. ncbi Urease production by Streptococcus thermophilus
    Teresa Zotta
    Dipartimento di Biologia, Difesa e Biotecnologie Agro Forestali, Universita degli Studi della Basilicata, Viale dell Ateneo Lucano 10, 85100 Potenza, Italy
    Food Microbiol 25:113-9. 2008
    In order to identify potential alternative sources of urease for the removal of urea from alcoholic beverages, 205 strains of lactic acid bacteria belonging to 27 different species were screened for urease production...
  34. pmc Mutagenesis of Klebsiella aerogenes UreG to probe nickel binding and interactions with other urease-related proteins
    Jodi L Boer
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA
    Biochemistry 49:5859-69. 2010
    UreG is a GTPase required for assembly of the nickel-containing active site of urease. Herein, a Strep-tagged Klebsiella aerogenes UreG (UreG(Str)) and selected site-directed variants of UreG(Str) were constructed for studying the in vivo ..
  35. ncbi Oral vaccination with liposome-encapsulated recombinant fusion peptide of urease B epitope and cholera toxin B subunit affords prophylactic and therapeutic effects against H. pylori infection in BALB/c mice
    Wenfeng Zhao
    School of Life Science and Technology, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China
    Vaccine 25:7664-73. 2007
    A new fusion peptide CtUBE of cholera toxin B subunit and Helicobacter pylori urease B subunit epitope was expressed in Escherichia coli. With this fusion peptide, an oral liposome vaccine against H...
  36. ncbi Helicobacter ganmani sp. nov., a urease-negative anaerobe isolated from the intestines of laboratory mice
    B R Robertson
    School of Microbiology and Immunology, The University of New South Wales, Sydney, Australia
    Int J Syst Evol Microbiol 51:1881-9. 2001
    ..Like Helicobacter rodentium, isolates possessed single, bipolar, unsheathed flagella and were urease-negative...
  37. pmc Unraveling the Helicobacter pylori UreG zinc binding site using X-ray absorption spectroscopy (XAS) and structural modeling
    Vlad Martin-Diaconescu
    Department of Chemistry, University of Massachusetts, Amherst, MA 01003, USA
    J Biol Inorg Chem 17:353-61. 2012
    The pathogenicity of Helicobacter pylori depends on the activity of urease for pH modification. Urease activity requires assembly of a dinickel active site that is facilitated in part by GTP hydrolysis by UreG...
  38. pmc Contribution of urease to colonization by Shiga toxin-producing Escherichia coli
    Susan R Steyert
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Infect Immun 80:2589-600. 2012
    ..The urease enzyme, encoded in the STEC chromosome, has been demonstrated to act as a virulence factor in other bacterial ..
  39. pmc Klebsiella aerogenes UreF: identification of the UreG binding site and role in enhancing the fidelity of urease activation
    Jodi L Boer
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, United States
    Biochemistry 51:2298-308. 2012
    The Ni-containing active site of Klebsiella aerogenes urease is assembled through the concerted action of the UreD, UreE, UreF, and UreG accessory proteins...
  40. ncbi Biochemical characterisation of urease from urease-positive thermophilic campylobacter (UPTC)
    A Tazumi
    Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Sagamihara, Japan
    Br J Biomed Sci 69:1-5. 2012
    This study aims to characterise biochemically urease from an atypical Campylobacter lari, namely urease-positive thermophilic Campylobacter (UPTC)...
  41. doi Chemical constituents of Stereospermum acuminatissimum and their urease and α-chymotrypsin inhibitions
    Kamdem Soup T Ramsay
    Department of Organic Chemistry, Higher Teachers Training College, University of Yaounde I, P O Box 47, Yaounde, Cameroon
    Fitoterapia 83:204-8. 2012
    ..The metabolites were screened for their potential against urease and α-chymotrypsin enzymes, as urease is targeted in peptic ulcer while α-chymotrypsin is used to remove protein ..
  42. doi Molecular modeling-based antioxidant arylidene barbiturates as urease inhibitors
    Khalid Mohammed Khan
    H E J Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan
    J Mol Graph Model 30:153-6. 2011
    Previously we have reported arylidene barbiturates 1-18 as a novel class of antioxidants; however, their urease inhibitory potential has not yet been explored...
  43. doi Large-scale virtual screening for the identification of new Helicobacter pylori urease inhibitor scaffolds
    Homa Azizian
    Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 16 Azar Ave, Tehran, Iran
    J Mol Model 18:2917-27. 2012
    ..pylori urease enzyme. One of the compounds selected showed urease inhibition in the low micromolar range...
  44. pmc Cryptococcal urease promotes the accumulation of immature dendritic cells and a non-protective T2 immune response within the lung
    John J Osterholzer
    Veterans Affairs Ann Arbor Health System, Ann Arbor, 2215 Fuller Rd, Ann Arbor, MI 48105, USA
    Am J Pathol 174:932-43. 2009
    b>Urease, a major virulence factor for Cryptococcus neoformans, promotes lethal meningitis/encephalitis in mice. The effect of urease within the lung, the primary site of most invasive fungal infections, is unknown...
  45. ncbi Chemistry and mechanism of urease inhibition
    Z Amtul
    HEJ Research Institute of Chemistry, International Center for Chemical Sciences, University of Karachi, Karachi, 75720, Pakistan
    Curr Med Chem 9:1323-48. 2002
    ..The enzyme inhibitors can interact with enzymes and block their activity towards natural substrates. Urease inhibitors have recently attracted much attention as potential new anti-ulcer drugs...
  46. pmc Wide-open flaps are key to urease activity
    Benjamin P Roberts
    Quantum Theory Project, University of Florida, P O Box 118435, Gainesville, Florida 32611 8435, USA
    J Am Chem Soc 134:9934-7. 2012
    Substrate ingress and product egress from the active site of urease is tightly controlled by an active-site flap...
  47. pmc Helicobacter pylori hydrogenase accessory protein HypA and urease accessory protein UreG compete with each other for UreE recognition
    Stéphane L Benoit
    Department of Microbiology, University of Georgia, 805 Biological Sciences Bldg, Athens, GA, USA
    Biochim Biophys Acta 1820:1519-25. 2012
    The gastric pathogen Helicobacter pylori relies on nickel-containing urease and hydrogenase enzymes in order to colonize the host...
  48. pmc Inhibition of Helicobacter pylori and associated urease by oregano and cranberry phytochemical synergies
    Y T Lin
    Department of Food Science, Chenoweth Laboratory, University of Massachusetts, Amherst, MA 01003, USA
    Appl Environ Microbiol 71:8558-64. 2005
    ..Subsequently, antimicrobial activity and antimicrobial-linked urease inhibition ability were evaluated...
  49. pmc Current tuberculosis diagnostic tools & role of urease breath test
    Mamoudou Maiga
    Center for Tuberculosis Research, Department of Medicine, Johns Hopkins University, MD, USA
    Indian J Med Res 135:731-6. 2012
    ..Our group has developed a rabbit urease breath test model to evaluate the sensitivity and the specificity of urease based detection of Mycobacterium ..
  50. doi Endoscopic tests for the diagnosis of Helicobacter pylori infection in children: Validation of rapid urease test
    Eleftheria Roma-Giannikou
    First Department of Pediatrics, University of Athens, Greece
    Helicobacter 15:227-32. 2010
    Rapid urease test (CLO-test) is an inexpensive and quick method for diagnosis of Helicobacter pylori infection with controversial results in children...
  51. pmc Role of rapid urease test and histopathology in the diagnosis of Helicobacter pylori infection in a developing country
    Javed Yakoob
    Department of Gastroenterology and Pathology, Aga Khan University Hospital, Karachi, Pakistan
    BMC Gastroenterol 5:38. 2005
    The aim of this study was to determine the effect of commonly self-prescribed proton pump inhibitors (PPI) on the results of rapid urease test and histology for the diagnosis of H. pylori infection.
  52. ncbi Analysis of Streptococcus salivarius urease expression using continuous chemostat culture
    Y Y Chen
    Department of Dental Research, University of Rochester School of Medicine and Dentistry, NY 14642, USA
    FEMS Microbiol Lett 135:223-9. 1996
    ..Using continuous chemostat culture, urease activity in Streptococcus salivarius 57...
  53. doi Design, synthesis, and biological evaluation of phosphoramide derivatives as urease inhibitors
    María J Domínguez
    Synthesis Section and Molecular Modeling Section, Department of Organic and Pharmaceutical Chemistry, University of Navarra, Irunlarrea 1, E 31008 Pamplona, Spain
    J Agric Food Chem 56:3721-31. 2008
    The design, synthesis, and biological evaluation of phosphoramide derivatives as urease inhibitors to reduce the loss of ammonia has been carried out...
  54. doi Diagnosis of Helicobacter pylori: improving the sensitivity of CLOtest by increasing the number of gastric antral biopsies
    Iqbal Siddique
    Department of Medicine, Faculty of Medicine, Kuwait University, Safat, Kuwait
    J Clin Gastroenterol 42:356-60. 2008
    The rapid urease CLOtest is commonly used during endoscopy to diagnose the presence of Helicobacter pylori...
  55. doi Biopsy site for detecting Helicobacter pylori infection in patients with gastric cancer
    Chan Gyoo Kim
    Research Institute and Hospital, National Cancer Center, Ilsandong gu, Goyang, Gyeonggi, Korea
    J Gastroenterol Hepatol 24:469-74. 2009
    ..pylori. The aim of this study was to determine appropriate biopsy site for detecting H. pylori in gastric cancer patients...
  56. doi Urease inhibitors as potential drugs for gastric and urinary tract infections: a patent review
    Paulina Kosikowska
    Wrocław University of Technology, Department of Bioorganic Chemistry, Faculty of Chemistry, Wrocław, Poland
    Expert Opin Ther Pat 21:945-57. 2011
    b>Urease is the enzyme that catalyzes the hydrolysis of urea, which is involved in serious infections caused by Helicobacter pylori in the gastric tract, as well as Proteus and related species in the urinary tract...
  57. doi Design, synthesis, and evaluation of novel organophosphorus inhibitors of bacterial ureases
    Stamatia Vassiliou
    Department of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Technology, Wyb Wyspianskiego 27, 50 370 Wrocław, Poland
    J Med Chem 51:5736-44. 2008
    A new group of organophosphorus inhibitors of urease, P-methyl phosphinic acids was discovered by using the structure based inhibitor design approach...
  58. ncbi Inhibition of urease by bismuth(III): implications for the mechanism of action of bismuth drugs
    Li Zhang
    Department of Chemistry and Open Laboratory of Chemical Biology, The University of Hong Kong, Pokfulam, Hong Kong, PR China
    Biometals 19:503-11. 2006
    ..b>Urease, an enzyme that converts urea into ammonia and carbonic acid, is crucial for colonization of the acidic ..
  59. ncbi Jackbean, soybean and Bacillus pasteurii ureases: biological effects unrelated to ureolytic activity
    Cristian Follmer
    Department of Biophysics, IB, and Graduate Program in Cellular and Molecular Biology, Center of Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
    Eur J Biochem 271:1357-63. 2004
    In this work we compared two plant ureases, jackbean urease (JBU) and embryo-specific soybean urease (SBU) and a bacterial (Bacillus pasteurii) urease, for kinetic parameters and other biological properties described recently for ureases ..
  60. doi Detection of Helicobacter pylori in adenotonsiller tissue specimens by rapid urease test and polymerase chain reaction
    Mete Eyigor
    Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey
    Eur Arch Otorhinolaryngol 266:1611-3. 2009
    ..Helicobacter pylori was searched by rapid urease test (RUT) and polymerase chain reaction (PCR). Presence of H...
  61. ncbi Ureases display biological effects independent of enzymatic activity: is there a connection to diseases caused by urease-producing bacteria?
    D Olivera-Severo
    Programa de Pós Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil
    Braz J Med Biol Res 39:851-61. 2006
    ..We showed that some isoforms of jack bean urease, canatoxin and the classical urease, bind to glycoconjugates and induce platelet aggregation...
  62. doi Urease from cotton (Gossypium hirsutum) seeds: isolation, physicochemical characterization, and antifungal properties of the protein
    Angela Menegassi
    Graduate Program in Molecular and Cellular Biology Center of Biotechnology and Department of Biophysics, Universidade Federal do Rio Grande do Sul UFRGS, Porto Alegre, RS CEP 91501 970, Brazil
    J Agric Food Chem 56:4399-405. 2008
    ..Despite an abundance of urease in vegetal tissues, the physiological role of this enzyme in plants is still poorly understood...
  63. pmc An intact urease assembly pathway is required to compete with NikR for nickel ions in Helicobacter pylori
    Erin L Benanti
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Bacteriol 191:2405-8. 2009
    We examined the effects of urease and hydrogenase assembly gene deletions on NikR activation in H. pylori strains 26695 and G27...
  64. pmc Urease is not involved in the virulence of Yersinia pseudotuberculosis in mice
    B Riot
    INSERM U411, Faculte de Medecine Necker, Paris, France
    Infect Immun 65:1985-90. 1997
    A chromosomal locus (ure) involved in the production of urease activity in the bacterial pathogen Yersinia pseudotuberculosis was characterized. The genetic organization of the Y...
  65. ncbi Jaburetox-2Ec: an insecticidal peptide derived from an isoform of urease from the plant Canavalia ensiformis
    F Mulinari
    Graduate Program in Cellular and Molecular Biology, Center of Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
    Peptides 28:2042-50. 2007
    Canatoxin, a urease isoform from Canavalia ensiformis seeds, shows insecticidal activity against different insect species...
  66. ncbi Antifungal activity of plant and bacterial ureases
    A B Becker-Ritt
    Graduate Program in Molecular and Cellular Biology, Center of Biotechnology, Universidade Federal do Rio Grande do Sul UFRGS, Brazil
    Toxicon 50:971-83. 2007
    ..Previous studies by our group have shown that plant ureases, but not a bacterial (Bacillus pasteurii) urease, display insecticidal activity...
  67. doi Membrane-disruptive properties of the bioinsecticide Jaburetox-2Ec: implications to the mechanism of the action of insecticidal peptides derived from ureases
    Pedro R Barros
    Departamento de Fisico Quimica, Instituto de Quimica, Universidade Federal do Rio de Janeiro, CT, Bloco A S410, Rio de Janeiro 21941 909, Brazil
    Biochim Biophys Acta 1794:1848-54. 2009
    Jaburetox-2Ec, a recombinant peptide derived from an urease isoform (JBURE-II), displays high insecticidal activity against important pests such as Spodoptera frugiperda and Dysdercus peruvianus...
  68. doi OmpR positively regulates urease expression to enhance acid survival of Yersinia pseudotuberculosis
    YangBo Hu
    Wuhan Institute of Virology, The Chinese Academy of Sciences, Wuhan 430071, PR China
    Microbiology 155:2522-31. 2009
    ..0 and pH 4.5 with DeltaompR at pH 7.0, and urease components were revealed to be the main targets for OmpR regulation...
  69. pmc Role of urease in megasome formation and Helicobacter pylori survival in macrophages
    Justin T Schwartz
    Department of Medicine, University of Iowa and the VA Medical Center, Iowa City, IA, USA
    J Leukoc Biol 79:1214-25. 2006
    ..Herein, we explored the role of Hp urease in bacterial survival in murine peritoneal macrophages and J774 cells...
  70. pmc Roles of alpha and beta carbonic anhydrases of Helicobacter pylori in the urease-dependent response to acidity and in colonization of the murine gastric mucosa
    Stéphanie Bury-Moné
    Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    Infect Immun 76:497-509. 2008
    ..physiology of Helicobacter pylori owing to its capnophilic nature, the large amounts of carbon dioxide produced by urease-mediated urea hydrolysis, and the constant bicarbonate supply in the stomach...
  71. ncbi Requirement of hydD, hydE, hypC and hypE genes for hydrogenase activity in Helicobacter pylori
    Stephane Benoit
    Department of Microbiology, 812 Biological Sciences Building, University of Georgia, Athens, GA 30602, USA
    Microb Pathog 36:153-7. 2004
    ..were all severely affected for hydrogenase activity, and in some cases (hypA and hypB mutants) also affected for urease activity. This finding prompted us to disrupt the two remaining unstudied hyp genes of H...
  72. ncbi Requirement of nickel metabolism proteins HypA and HypB for full activity of both hydrogenase and urease in Helicobacter pylori
    J W Olson
    Department of Microbiology, 813 Biological Sciences Building, University of Georgia, Athens, GA 30602, USA
    Mol Microbiol 39:176-82. 2001
    The nickel-containing enzymes hydrogenase and urease require accessory proteins in order to incorporate properly the nickel atom(s) into the active sites...
  73. pmc Attenuated Salmonella enterica serovar Typhi expressing urease effectively immunizes mice against Helicobacter pylori challenge as part of a heterologous mucosal priming-parenteral boosting vaccination regimen
    Patricia Londoño-Arcila
    Acambis Ltd, Cambridge, United Kingdom
    Infect Immun 70:5096-106. 2002
    Recombinant vaccine strains of Salmonella enterica serovar Typhi capable of expressing Helicobacter pylori urease were generated by transforming strains CVD908 and CVD908-htrA with a plasmid harboring the ureAB genes under the control of ..
  74. pmc The Helicobacter pylori UreI protein is not involved in urease activity but is essential for bacterial survival in vivo
    S Skouloubris
    Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, 75724 Paris Cedex 15, France
    Infect Immun 66:4517-21. 1998
    ..Helicobacter pylori ureI mutants to investigate the function of UreI, the product of one of the genes of the urease cluster...
  75. doi Inverse nickel-responsive regulation of two urease enzymes in the gastric pathogen Helicobacter mustelae
    Jeroen Stoof
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Environ Microbiol 10:2586-97. 2008
    ..be chronically colonized by pathogenic Helicobacter species, which use the nickel-dependent urea-degrading enzyme urease to confer acid resistance...
  76. ncbi Endoscopic diagnosis of Helicobacter pylori infection by rapid urease test in bleeding peptic ulcers: a prospective case-control study
    Jui Hsiang Tang
    Department of Gastroenterology and Hepatology, Chang Gung Memorial Hospital, Taipei, Taiwan
    J Clin Gastroenterol 43:133-9. 2009
    To assess the efficacy of rapid urease test (RUT) in patients with bleeding ulcers, as well as the effects of visible blood in the stomach and short-term (<24 h) use of standard-dose proton pump inhibitor (PPI) on RUT sensitivity.
  77. pmc Silencing and reactivation of urease in Yersinia pestis is determined by one G residue at a specific position in the ureD gene
    F Sebbane
    Equipe Inserm E9919 Université JE2225, Département de Pathogenèse des Maladies Infectieuses et Parasitaires, Institut de Biologie de Lille, 59021 Lille, France
    Infect Immun 69:170-6. 2001
    ..the plague agent, is a naturally nonureolytic microorganism, while all other Yersinia species display a potent urease activity. In this report we demonstrate that Y...
  78. ncbi Alkali production by oral bacteria and protection against dental caries
    R A Burne
    Center for Oral Biology and Department of Microbiology and Immunology, University of Rochester Medical Center, Box 611, 601 Elmwood Ave, Rochester, NY 14642, USA
    FEMS Microbiol Lett 193:1-6. 2000
    ..This short review highlights recent progress on understanding molecular genetic and physiologic aspects of ammonia generation by prominent oral bacteria...
  79. pmc Identification of the nik gene cluster of Brucella suis: regulation and contribution to urease activity
    V Jubier-Maurin
    Institut National de la Santé et de la Recherche Médicale U 431, Institut E Bataillon, Universite Montpellier II, 34095 Montpellier, France
    J Bacteriol 183:426-34. 2001
    ..Insertional inactivation of nikA strongly reduced the activity of the nickel metalloenzyme urease, which was restored by addition of a nickel excess. Moreover, the nikA mutant of B...
  80. pmc Nickel-based Enzyme Systems
    Stephen W Ragsdale
    Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109 0606, USA
    J Biol Chem 284:18571-5. 2009
    ..The metal centers vary from mononuclear to complex metal clusters and catalyze simple hydrolytic to multistep redox reactions...
  81. pmc Mice are protected from Helicobacter pylori infection by nasal immunization with attenuated Salmonella typhimurium phoPc expressing urease A and B subunits
    I E Corthesy-Theulaz
    Department of Internal Medicine CHUV, and Institute of Pathology, Lausanne University, Switzerland
    Infect Immun 66:581-6. 1998
    ..The genes encoding the A and B subunits of H. pylori urease were introduced into S...
  82. pmc Characterization of Helicobacter pylori nickel metabolism accessory proteins needed for maturation of both urease and hydrogenase
    Nalini Mehta
    Department of Microbiology, 527 Biological Sciences Building, University of Georgia, Athens, GA 30602, USA
    J Bacteriol 185:726-34. 2003
    ..that two accessory proteins, HypA and HypB, play a role in nickel-dependent maturation of both hydrogenase and urease in Helicobacter pylori. Here, the two proteins were purified and characterized...
  83. ncbi Description of 'Candidatus Helicobacter heilmannii' based on DNA sequence analysis of 16S rRNA and urease genes
    Jani L O'Rourke
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia, 2052
    Int J Syst Evol Microbiol 54:2203-11. 2004
    ..gastrospirillum isolates from humans and animals, which incorporates sequence data based on the 16S rRNA and urease genes...
  84. pmc Cytoplasmic histidine kinase (HP0244)-regulated assembly of urease with UreI, a channel for urea and its metabolites, CO2, NH3, and NH4(+), is necessary for acid survival of Helicobacter pylori
    David R Scott
    Department of Physiology, David Geffen School of Medicine at UCLA, 405 Hilgard Ave, Los Angeles, California 90024, USA
    J Bacteriol 192:94-103. 2010
    ..b>Urease activity, urea flux through the pH-gated urea channel, UreI, and periplasmic alpha-carbonic anhydrase are ..
  85. ncbi Oral immunization of mice with lactic acid bacteria producing Helicobacter pylori urease B subunit partially protects against challenge with Helicobacter felis
    Blaise Corthesy
    R and D Laboratory of the Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    J Infect Dis 192:1441-9. 2005
    ..Since the use of mucosal adjuvants is limited in human application, we have evaluated the potential of recombinant Lactobacillus strains producing H. pylori urease B (UreB) subunit to deliver this antigen to the gastrointestinal tract.
  86. ncbi Supramolecular assembly and acid resistance of Helicobacter pylori urease
    N C Ha
    National Creative Research Initiative Center for Biomolecular Recognition, Department of Life Science and Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang, Kyungbuk, 790 784, Korea
    Nat Struct Biol 8:505-9. 2001
    Helicobacter pylori, an etiologic agent in a variety of gastroduodenal diseases, produces a large amount of urease, which is believed to neutralize gastric acid by producing ammonia for the survival of the bacteria...
  87. ncbi Transcriptional and mutational analysis of the Helicobacter pylori urease promoter
    Beverly J Davies
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK
    FEMS Microbiol Lett 213:27-32. 2002
    b>Urease is an essential virulence factor of the human gastric pathogen Helicobacter pylori, and is expressed to very high levels...
  88. pmc Shuttle cloning and nucleotide sequences of Helicobacter pylori genes responsible for urease activity
    A Labigne
    Unité des Entérobactéries, INSERM U199, Institut Pasteur, Paris, France
    J Bacteriol 173:1920-31. 1991
    Production of a potent urease has been described as a trait common to all Helicobacter pylori so far isolated from humans with gastritis as well as peptic ulceration. The detection of urease activity from genes cloned from H...
  89. pmc Establishment of systemic Brucella melitensis infection through the digestive tract requires urease, the type IV secretion system, and lipopolysaccharide O antigen
    Tatiane A Paixao
    Department of Medical Microbiology and Immunology, University of California at Davis, Davis, CA 95616, USA
    Infect Immun 77:4197-208. 2009
    ..In this study, a mouse model of oral infection was characterized to assess the roles of urease, the VirB type IV secretion system, and lipopolysaccharide for establishing infection through the digestive tract...
  90. pmc Correlations of oral bacterial arginine and urea catabolism with caries experience
    M M Nascimento
    Department of Operative Dentistry, College of Dentistry, University of Florida, Gaineseville, FL 32610, USA
    Oral Microbiol Immunol 24:89-95. 2009
    ..if the capacity of oral samples to produce ammonia from arginine or urea was related to caries experience, the arginine deiminase system (ADS) and urease activity in saliva and dental plaque samples were measured in 45 adult subjects.
  91. pmc Transcriptional regulation of the Streptococcus salivarius 57.I urease operon
    Y Y Chen
    Center for Oral Biology, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA
    J Bacteriol 180:5769-75. 1998
    ..Thus, it was concluded that PureI was the predominant, regulated promoter and that regulation was governed by a mechanism differing markedly from other known mechanisms for bacterial urease expression.
  92. doi Prospective evaluation of a new stool antigen test for the detection of Helicobacter pylori, in comparison with histology, rapid urease test, (13)C-urea breath test, and serology
    Jeongmin Choi
    Department of Internal Medicine and Healthcare Research Institute, Seoul National University Hospital Healthcare System Gangnam Center, Seoul, South Korea
    J Gastroenterol Hepatol 26:1053-9. 2011
    ..This study aimed to evaluate the efficacy of a new polyclonal enzyme immunoassay for the detection of Helicobacter pylori (H. pylori) antigen in stool by determination of the optimal cut-off value in the screening population...
  93. pmc Interallelic complementation at the ubiquitous urease coding locus of soybean
    Ariel Goldraij
    Department of Biochemistry, University of Missouri, Columbia, Missouri 65211, USA
    Plant Physiol 132:1801-10. 2003
    Soybean (Glycine max [L.] Merrill) mutant aj6 carries a single recessive lesion, aj6, that eliminates ubiquitous urease activity in leaves and callus while retaining normal embryo-specific urease activity...
  94. pmc Surface properties of Helicobacter pylori urease complex are essential for persistence
    Tobias D Schoep
    Ondek Pty Ltd and H pylori Research Laboratory, Microbiology and Immunology, University of Western Australia, Nedlands, Australia
    PLoS ONE 5:e15042. 2010
    The enzymatic activity of Helicobacter pylori's urease neutralises stomach acidity, thereby promoting infection by this pathogen...
  95. doi Crystalline bacterial biofilm formation on urinary catheters by urease-producing urinary tract pathogens: a simple method of control
    Robert J Broomfield
    Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK
    J Med Microbiol 58:1367-75. 2009
    The problem of catheter encrustation stems from infection by urease-producing bacteria...
  96. ncbi The marine cyanobacterium Synechococcus sp. WH7805 requires urease (urea amidohydrolase, EC 3.5.1.5) to utilize urea as a nitrogen source: molecular-genetic and biochemical analysis of the enzyme
    J L Collier
    Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093 0202, USA
    Microbiology 145:447-59. 1999
    ..Oligonucleotide primers were used to amplify a conserved fragment of the urease (urea amidohydrolase, EC 3.5.1.5) coding region from cyanobacteria. A 5...
  97. ncbi A H+-gated urea channel: the link between Helicobacter pylori urease and gastric colonization
    D L Weeks
    VA Greater Los Angeles Healthcare System and Department of Physiology, University of California, Los Angeles, CA 90073, USA
    Science 287:482-5. 2000
    Acidic media trigger cytoplasmic urease activity of the unique human gastric pathogen Helicobacter pylori. Deletion of ureI prevents this activation of cytoplasmic urease that is essential for bacterial acid resistance...
  98. doi Effect of subinhibitory concentrations of cumin (Cuminum cyminum L.) seed essential oil and alcoholic extract on the morphology, capsule expression and urease activity of Klebsiella pneumoniae
    Safoura Derakhshan
    Department of Bacteriology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
    Int J Antimicrob Agents 32:432-6. 2008
    ..the effect of subminimum inhibitory concentrations (sub-MICs) on cell morphology, capsule expression and urease activity. Growth of K. pneumoniae strains exposed to sub-MICs of C...
  99. pmc Motility of urease-deficient derivatives of Helicobacter pylori
    Shumin Tan
    Department of Molecular Microbiology, Washington University Medical School, St Louis, Missouri 63110, USA
    J Bacteriol 186:885-8. 2004
    Early studies of a ureB mutant derivative of Helicobacter pylori had suggested that urease is needed for motility and that urease action helps energize flagellar rotation...
  100. doi Characterization of NikR-responsive promoters of urease and metal transport genes of Helicobacter mustelae
    Jeroen Stoof
    Department of Gastroenterology and Hepatology, Erasmus MC, University Medical Center, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Biometals 23:145-59. 2010
    ..in the gastric pathogen Helicobacter pylori controls expression of nickel-transporters and the nickel-cofactored urease acid resistance determinant...
  101. doi Why urease is a di-nickel enzyme whereas the CcrA β-lactamase is a di-zinc enzyme
    Crystal E Valdez
    Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095 1569, United States
    J Phys Chem B 116:10649-56. 2012
    ..Five enzyme/substrate systems are considered: urease/urea, CcrA β-lactamase/β-lactam antibiotic model, urease/β-lactam antibiotic model, CcrA β-lactamase/urea, and ..

Research Grants62

  1. Regulation of Host Nitrogen Balance by the Gut Microbiome
    Gary D Wu; Fiscal Year: 2012
    ..Urea, produced by the host, is hydrolyzed in the colon by urease-producing gut bacteria and the resultant ammonia is absorbed by the host and utilized for protein metabolism...
  2. Acid Adaptation Targets for Eradication of Helicobacter pylori
    George Sachs; Fiscal Year: 2013
    ..The system responds by acid-induced transcriptional upregulation of two promoters in the urease gene cluster, pureA and pureI, acid-induced post-translational upregulation of assembly UreI with apourease and ..
  3. Structure-Function Studies of the Proton-Gated Urea Channel from H. Pylori
    Hartmut Luecke; Fiscal Year: 2013
    ..HpUreI is a proton-gated urea channel necessary for rapid access of urea to intrabacterial urease, essential for maintaining the periplasm at pH 6.1 in the acidic environment of the stomach, as low as pH 2...
  4. Urease Activity and Nitrogen Assimilation in Helicobacter pylori
    ERICA FRANCESCA MILLER; Fiscal Year: 2012
    ..b>Urease is an enzyme that is required for infection by H...
  5. The Gastric Biology of Helicobacter Pylori
    George Sachs; Fiscal Year: 2012
    ..to dual therapy with a long acting PPI + amoxicillin;(C) Investigate pH-induced activation and trafficking of a urease complex and other proteins to UreI on the inner membrane and determine whether this is regulated by HP0165 or ..
  6. Breath Test for Pseudomonas aeruginosa in CF
    Graham S Timmins; Fiscal Year: 2010
    ..isotope ratio mass spectrometry to measure isotopically labeled gasses from denitrification, cyanide synthase and urease activities in P. aeruginosa in vitro...
  7. BACTERIAL UREASE IN THE CATHETERIZED URINARY TRACT
    HARRY MOBLEY; Fiscal Year: 1990
    ..Nearly half (47%) of the gram-negative bacilli isolated produced the enzyme urease which has been implicated in pyelonephritis, hepatic coma, hyperammonemia, complement inactivation, and kidney and ..
  8. Immunobiology and Prevention of H. pylori Disease
    Phillip Smith; Fiscal Year: 2009
    ..2) In a mouse model that recapitulates human H. pylori infection, H. pylori urease (or other antigens) drives an IL-10/TGF-( dominated Tr response in young animals but a predominant Thl response in ..
  9. BIOLOGICAL METAL CLUSTERS--BIOPHYSICAL AND MODEL STUDIES
    Michael Maroney; Fiscal Year: 1999
    ..g. photosystem II, catalase), Ni atoms (urease), and more than one kind of metal, or heteropolynuclear clusters (e.g...
  10. Virulence Factor Trafficking in Cryptococcus
    Peter Williamson; Fiscal Year: 2005
    ..Each of the mutants identified in Specific Aims 2 and 3 will then be assessed for production of other virulence-related factors such as capsule, urease, phospholipase and virulence in a mouse model.
  11. Nanosensor device for the detection of H. Pylori and prevention of gastric cancer
    ANASTASIA RIGAS; Fiscal Year: 2012
    ..It is based on the ability of H pylori to convert urea to CO2 and ammonia using its enzyme urease. Current embodiments of UBT employ 13C-urea and detect labeled CO2 in breath...
  12. CAMPYLOBACTER PYLORIDIS IN GASTRODUODENAL DISEASE
    David Graham; Fiscal Year: 1991
    ..We have developed and validated a reliable noninvasive breath test based on the high endogenous urease content of C. pyloridis. This test uses 13C-urea and detects the presence of C...
  13. Urea-dependent virulence in uropathogenic bacteria
    CARLEEN COLLINS; Fiscal Year: 2004
    DESCRIPTION(provided by applicant: Urease, which catalyzes the hydrolysis of urea to ammonia and carbonic acid is produced by diverse bacterial species including various aerobes, facultative anaerobes and obligate anaerohes Both Gram-..
  14. Alkali production in human dental plaque and saliva as predictor of caries risk
    VALERIA GORDAN; Fiscal Year: 2009
    ..The objective of this study is to examine the relationship of arginine deiminase (AD) and urease activities with dental caries status at the clinical level...
  15. NATURAL AND INDUCED IMMUNITY AGAINST HELICOBACTER PYLORI
    Pierre Michetti; Fiscal Year: 2000
    ..Using H. felis infection and urease-based immunization as models, this proposal aims at delineating the origin and the homing pattern of the immune ..
  16. NATURAL AND INDUCED IMMUNITY AGAINST HELICOBACTER PYLORI
    Pierre Michetti; Fiscal Year: 2001
    ..Using H. felis infection and urease-based immunization as models, this proposal aims at delineating the origin and the homing pattern of the immune ..
  17. H.pylori arginase modulation of ulcers and cancer
    David McGee; Fiscal Year: 2007
    ..H. pylori exerts an enormous amount of cellular energy on nitrogen metabolism including the urease enzyme. Therefore, the critical role of urease in virulence has been a major research focus. However, H...
  18. Development of a Urea Biosensor Using the UreR Protein
    HARRY MOBLEY; Fiscal Year: 2004
    ..The sensor will employ the UreR protein encoded by the urease operon of the uropathogenic bacterial species Proteus mirabilis...
  19. BACTERIAL UREASE IN UTI
    HARRY MOBLEY; Fiscal Year: 2000
    ..Urea, the principal nitrogenous waste product of humans (present at nearly 0.5 M in urine) induces urease synthesis in P. mirabilis and is hydrolyzed by this enzyme...
  20. NOVEL E. COLI 0157:H7 INTESTINAL COLONIZATION FACTORS
    JAMES KAPER; Fiscal Year: 2009
    ..with wild type EHEC and isogenic effector mutants using in vitro organ culture (IVOC);3) Characterize the role of urease in pathogenesis and environmental persistence;and 4) Characterize transcriptional of the LEE4 operon, which ..
  21. ACID INDUCIBLE RESPONSES IN H PYLORI
    Catherine McGowan; Fiscal Year: 2003
    ..b>Urease activity is required for H. pylori survival at low pH, and its presence is essential for colonization...
  22. S.T.R.A.P Sonos Transportable Renal Application Product
    Martin Roberts; Fiscal Year: 2005
    ..The Specific Aims are: 1) Immobilize urease to hydrolize urea, the major waste eliminated by the kidney, 2) Develop a cation exchange unit based on zirconium ..
  23. PHOP/PHOQ DELETED S TYPHI VACCINE STRAINS
    Elizabeth Hohmann; Fiscal Year: 2002
    ..typhi vaccine strain expressing cytoplasmic Helicobacter pylori urease followed by purified recombinant urease and E...
  24. COMMUNITY COMPOSITION AND PHYSIOLOGY OF ORAL BIOFILMS
    David White; Fiscal Year: 2001
    ..blocks" will be defined by characterizing enzymatic activities (proteases, mucin-specific exoglycosidases, urease) as indicators of nutrient-protein catabolism, mucin catabolism, and urea hydrolysis; the rate of glucose ..
  25. MECHANISMS OF UREASE SURFACE LOCALIZATION IN H PYLORI
    Bruce Dunn; Fiscal Year: 1999
    ..H. pylori expresses significant urease activity which is an essential virulence factor...
  26. CAMPYLOBACTER PYLORI-INDUCED GASTRIC DISEASE
    Steven Krakowka; Fiscal Year: 1992
    ..One these techniques are mastered we will determine the role of selected bacterial virulence factors (urease and motility) in the pathogenesis of gastritis in a series of short-using developed endoscopic, immunologic and ..
  27. CAMPYLOBACTER PYLORI IN GASTROINTESTINAL DISEASE
    JOHN PENNER; Fiscal Year: 1990
    ..Because of its known importance the urease enzyme will be given special attention in these studies...
  28. STUDIES OF SYNTHETIC METALLOENZYME MIMICS
    CYNTHIA BURROWS; Fiscal Year: 1991
    ..In the area of hydrolysis, studies mimicking the proposed mechanism of urease-catalyzed hydrolysis will be performed...
  29. C. Neoformans Infection in Organ Transplant Recipients
    Nina Singh; Fiscal Year: 2005
    ..with clinical manifestations and outcome: capsule formation, serotype, melanin synthesis, thermal susceptibility, urease, and phospholipase production. 4. To determine if C...
  30. REGULATION OF GLUTAMINE SYNTHETASE IN STREPTOMYCES
    Susan Fisher; Fiscal Year: 1993
    ..Since expression of the nitrogen-regulated enzyme urease is also deficient in these mutants, a global nitrogen regulatory system may be present in .1. coelicolor...
  31. SITE SPECIFIC MUTAGENESIS OF H PYLORI CARBONIC ANHYDRASE
    ROGER ROWLETT; Fiscal Year: 1999
    ..of carbonic anhydrase in processing cellular carbon dioxide, bicarbonate and protons--all products of the enzyme urease which H...
  32. UREASE AND DEAMINASES IN CHEMISTRY AND MEDICINE
    William Fishbein; Fiscal Year: 1980
    ..With regard to urease, concentrated effort will be focused on obtaining accurate subunit and metal ion stoichiometries by accurate ..
  33. NEUROTRANSMITTERS, APPETITE & INBORN METABOLISM ERRORS
    Mark Batshaw; Fiscal Year: 1991
    ..behavior and serotonin metabolism will also be studied in three animal models associated with hyperammonemia: urease infused rats, valproate treated rats, and sparse fur mice...
  34. Urease Activity and Dental Caries in Children
    EVANGELIA MOROU BERMUDEZ; Fiscal Year: 2009
    ..application consists of a combined clinical and basic research study, which aims to address the role bacterial urease activity in the development of dental caries in children...
  35. Disposable sensor for on-line measure of urea clearance in effluent dialysate
    Patrick Grant; Fiscal Year: 2006
    ..1). Develop a nanoassembly process to repeatably deposit a stable pH sensing film and urease on the inner wall of standard dialysis tubing. (2)...
  36. GENES OF H PYLORI ESSENTIAL FOR GASTRIC COLONIZATION
    Andrew Plaut; Fiscal Year: 1999
    ..pylori. The first approach has involved the construction of a novel urease reporter system to identify acid-inducible genes in vitro, and identification of genes whose expression is induced ..
  37. HELICOBACTER PYLORI UREASE ORAL VACCINE
    William Thomas; Fiscal Year: 1993
    The goal of this proposal is to determine the feasibility of oral vaccination with recombinant Helicobacter pylori urease. This goal will be tested by the oral immunization of germ and specific pathogen free mice with recombinant urease ..
  38. A NOVEL METHOD TO DETECT HELICOBACTER PYLORI IN CHILDREN
    David Wagner; Fiscal Year: 2004
    ..The Ez-HBT(TM) is superior to antibody based tests because it detects the presence of gastric urease activity. Urease activity is only present when H. pylori have colonized the stomach...
  39. NEW MATERIALS FOR URINARY CATHETERS AND STENTS
    SHANTHA SARANGAPANI; Fiscal Year: 1990
    ..The encrustation is caused by urease producing bacteria: the action of urease on urea results in ammonia production, resulting in increased urine pH...
  40. A NOVEL METHOD TO DETECT HELICOBACTER PYLORI IN CHILDREN
    David Wagner; Fiscal Year: 2000
    ..pylori infection in adults based on the bacteria's urease activity to split urea to carbon dioxide. A small dose of 13C-urea is administered and if H...
  41. HUS Pathogenesis & clinical Outcome in an in vivo model
    James Fox; Fiscal Year: 2008
    ..We will use this model to study the pathogenesis of HUS and importantly to develop therapeutic regimens to prevent and treat EHEC disease. [unreadable] [unreadable] [unreadable] [unreadable]..
  42. Helicobacter induced hepatitis and tumorigenesis
    James Fox; Fiscal Year: 2009
    ..A more complete understanding of the etiopathogenesis of hepatobiliary disease and hepatocellular carcinoma is likely to result in new strategies for treatment and prevention of these important human diseases. ..
  43. UREASE AND GENE EXPRESSION OF HELICOBACTER PYLORI
    HARRY MOBLEY; Fiscal Year: 2008
    ..highly restricted to the gastric mucosa of humans, has adopted a strategy of survival that includes synthesis of urease as its most abundant cellular protein...
  44. IN VIVO PATHOGENESIS OF HELICOBACTER PYLORI
    James Fox; Fiscal Year: 2009
    ..pylori pathogenesis and specifically evaluate the role of regulatory T cells in limiting H. pylori-induced gastric pathology. ..
  45. DEVELOPING AND IMPROVING INSTITUTIONAL ANIMAL RESOURCES
    James Fox; Fiscal Year: 2006
    ....
  46. Microecology-murine gut-initiation & progression of IBD
    James Fox; Fiscal Year: 2005
    ..prior and subsequent to oral vaccination with Helicobacter spp. antigens and mucosal adjuvants, elucidate how these vaccine strategies influence microflora dynamics and impact gut cytokine responses. ..
  47. THE GASTRIC ACID PUMP AS A TARGET FOR ULCER TREATMENT
    George Sachs; Fiscal Year: 2009
    ..The results of the proposed research will further improve the agents used for the treatment of acid-related diseases and also our knowledge of the ATPase and cellular events involved in regulation of its activity. ..
  48. GASTRIC ENTEROCHROMAFFIN-LIKE CELL IN ULCER DISEASE
    George Sachs; Fiscal Year: 2005
    ..Hence this approach will provide additional insights into mechanisms of regulation of gastric secretion and enable illuminated interpretation of the pathophysiology of peptic ulcer disease. ..
  49. CARDIOPROTECTIVE EFFECT--NEW CHELATORS FOR THALASSEMIA
    Chaim Hershko; Fiscal Year: 2001
    ..abstract_text> ..
  50. Nitroxyl and Nitric Oxide Producing Reactions of Hydroxyurea and Related Compound
    S Bruce King; Fiscal Year: 2010
    ..The results from these studies should enhance our ability to use HNO/NO donors as treatments for sickle cell disease, other anemias and congestive heart failure. . ..
  51. Repair of methionine residues in H. pylori catalase
    Robert Maier; Fiscal Year: 2008
    ..It is proposed to understand what factors influence the need for this repair in a key protein. [unreadable] [unreadable] [unreadable] [unreadable]..
  52. Development of a vaccine against Helicobacter pylori to prevent gastric cancer
    RODOLFO BEGUE; Fiscal Year: 2008
    ..The specific aims of this application are: a) to construct 3 candidate products based on H. pylori urease B: purified recombinant protein (done), DNA plasmid (done) and HBsAg-urease B (this application); and b) to test ..
  53. Nickel Metabolism in Helicobacter pylori
    Robert Maier; Fiscal Year: 2006
    ..Two nickel-containing enzymes, urease and hydrogenase, are important for the bacterium's mucosal-colonizing abilities...
  54. Helicobacter pylori: Pathogenesis and Virulence
    Kenneth Simpson; Fiscal Year: 2005
    ....
  55. Oxidative Stress and Survival of Helicobacter pylori
    Robert Maier; Fiscal Year: 2005
    ....
  56. Virulence Determinants in Helicobacter hepaticus
    Robert Maier; Fiscal Year: 2004
    ..If specific mutants can be created, then virulence determinants can begin to be systematically studied. ..
  57. 4th Western Pacific Helicobacter Congress
    Barry Marshall; Fiscal Year: 2002
    ..Concurrent sessions will allow delegates to prioritise their sessions. ..