prothrombin

Summary

Summary: A plasma protein that is the inactive precursor of thrombin. It is converted to thrombin by a prothrombin activator complex consisting of factor Xa, factor V, phospholipid, and calcium ions. Deficiency of prothrombin leads to hypoprothrombinemia.

Top Publications

  1. ncbi Prothrombin/thrombin and the thrombin receptors PAR-1 and PAR-4 in the brain: localization, expression and participation in neurodegenerative diseases
    Elena Sokolova
    Otto von Guericke Universitaet Magdeburg, Medizinische Fakultat, Institut fur Neurobiochemie, Leipziger Strasse 44, Magdeburg, Germany
    Thromb Haemost 100:576-81. 2008
  2. ncbi p38 MAPK controls prothrombin expression by regulated RNA 3' end processing
    Sven Danckwardt
    Department of Pediatric Oncology, Hematology, and Immunology, University of Heidelberg, Heidelberg, Germany
    Mol Cell 41:298-310. 2011
  3. pmc Prothrombin deficiency results in embryonic and neonatal lethality in mice
    W Y Sun
    Division of Developmental Biology, Children s Hospital Research Foundation, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 95:7597-602. 1998
  4. ncbi A common genetic variation in the 3'-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis
    S R Poort
    Hemostasis and Thrombosis Research Center, Leiden University Hospital, The Netherlands
    Blood 88:3698-703. 1996
  5. pmc Des-gamma-carboxy prothrombin and alpha-fetoprotein as biomarkers for the early detection of hepatocellular carcinoma
    Anna S Lok
    Division of Gastroenterology, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA
    Gastroenterology 138:493-502. 2010
  6. ncbi D-dimer and prothrombin fragment 1 + 2 predict venous thromboembolism in patients with cancer: results from the Vienna Cancer and Thrombosis Study
    Cihan Ay
    Clinical Division of Haematology and Haemostaseology, Department of Medicine I, Medical University of Vienna, Waehringer GuerA 1090 Vienna, Austria
    J Clin Oncol 27:4124-9. 2009
  7. pmc Goal-directed coagulation management of major trauma patients using thromboelastometry (ROTEM)-guided administration of fibrinogen concentrate and prothrombin complex concentrate
    Herbert Schöchl
    Department of Anaesthesiology and Intensive Care, AUVA Trauma Hospital, Dr Franz Rehrl Platz 5, Salzburg, Austria
    Crit Care 14:R55. 2010
  8. ncbi Reversal of dabigatran anticoagulation by prothrombin complex concentrate (Beriplex P/N) in a rabbit model
    I Pragst
    CSL Behring GmbH, Marburg Boehringer Ingelheim Pharma GmbH and Co KG, Biberach, Germany
    J Thromb Haemost 10:1841-8. 2012
  9. ncbi Significance of alpha-fetoprotein and des-gamma-carboxy prothrombin in patients with hepatocellular carcinoma undergoing hepatectomy
    Kentaroh Yamamoto
    Department of Surgery, Hepato Biliary Pancreatic Surgery Division, Artificial Organ and Transplantation Division, University of Tokyo, Tokyo, Japan
    Ann Surg Oncol 16:2795-804. 2009
  10. ncbi Increased efficiency of mRNA 3' end formation: a new genetic mechanism contributing to hereditary thrombophilia
    N H Gehring
    Department of Pediatrics, , Humboldt University, Berlin, Germany
    Nat Genet 28:389-92. 2001

Detail Information

Publications327 found, 100 shown here

  1. ncbi Prothrombin/thrombin and the thrombin receptors PAR-1 and PAR-4 in the brain: localization, expression and participation in neurodegenerative diseases
    Elena Sokolova
    Otto von Guericke Universitaet Magdeburg, Medizinische Fakultat, Institut fur Neurobiochemie, Leipziger Strasse 44, Magdeburg, Germany
    Thromb Haemost 100:576-81. 2008
    ..Both prothrombin and its active form thrombin have been detected locally in the brain...
  2. ncbi p38 MAPK controls prothrombin expression by regulated RNA 3' end processing
    Sven Danckwardt
    Department of Pediatric Oncology, Hematology, and Immunology, University of Heidelberg, Heidelberg, Germany
    Mol Cell 41:298-310. 2011
    ..Although induced in many (patho-)physiological conditions, the underlying mechanisms controlling prothrombin expression remained enigmatic...
  3. pmc Prothrombin deficiency results in embryonic and neonatal lethality in mice
    W Y Sun
    Division of Developmental Biology, Children s Hospital Research Foundation, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 95:7597-602. 1998
    The conversion of prothrombin (FII) to the serine protease, thrombin (FIIa), is a key step in the coagulation cascade because FIIa triggers platelet activation, converts fibrinogen to fibrin, and activates regulatory pathways that both ..
  4. ncbi A common genetic variation in the 3'-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis
    S R Poort
    Hemostasis and Thrombosis Research Center, Leiden University Hospital, The Netherlands
    Blood 88:3698-703. 1996
    We have examined the prothrombin gene as a candidate gene for venous thrombosis in selected patients with a documented familial history of venous thrombophilia...
  5. pmc Des-gamma-carboxy prothrombin and alpha-fetoprotein as biomarkers for the early detection of hepatocellular carcinoma
    Anna S Lok
    Division of Gastroenterology, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA
    Gastroenterology 138:493-502. 2010
    ..The aim of this study was to compare the accuracy of alpha-fetoprotein (AFP) and des-gamma-carboxy prothrombin (DCP) in the early diagnosis of HCC.
  6. ncbi D-dimer and prothrombin fragment 1 + 2 predict venous thromboembolism in patients with cancer: results from the Vienna Cancer and Thrombosis Study
    Cihan Ay
    Clinical Division of Haematology and Haemostaseology, Department of Medicine I, Medical University of Vienna, Waehringer GuerA 1090 Vienna, Austria
    J Clin Oncol 27:4124-9. 2009
    ..The aim of this study was to investigate D-dimer and prothrombin fragment 1 + 2 (F 1 + 2), which reflect activation of blood coagulation and fibrinolysis, for prediction of ..
  7. pmc Goal-directed coagulation management of major trauma patients using thromboelastometry (ROTEM)-guided administration of fibrinogen concentrate and prothrombin complex concentrate
    Herbert Schöchl
    Department of Anaesthesiology and Intensive Care, AUVA Trauma Hospital, Dr Franz Rehrl Platz 5, Salzburg, Austria
    Crit Care 14:R55. 2010
    ..The appropriate strategy for trauma-induced coagulopathy management is under debate. We report the treatment of major trauma using mainly coagulation factor concentrates...
  8. ncbi Reversal of dabigatran anticoagulation by prothrombin complex concentrate (Beriplex P/N) in a rabbit model
    I Pragst
    CSL Behring GmbH, Marburg Boehringer Ingelheim Pharma GmbH and Co KG, Biberach, Germany
    J Thromb Haemost 10:1841-8. 2012
    ..b>Prothrombin complex concentrates (PCCs) have served as a standard treatment for the reversal of coumarin anticoagulation.
  9. ncbi Significance of alpha-fetoprotein and des-gamma-carboxy prothrombin in patients with hepatocellular carcinoma undergoing hepatectomy
    Kentaroh Yamamoto
    Department of Surgery, Hepato Biliary Pancreatic Surgery Division, Artificial Organ and Transplantation Division, University of Tokyo, Tokyo, Japan
    Ann Surg Oncol 16:2795-804. 2009
    Alpha-fetoprotein (AFP) and des-gamma-carboxy prothrombin (DCP) are well-known tumor markers of hepatocellular carcinoma (HCC)...
  10. ncbi Increased efficiency of mRNA 3' end formation: a new genetic mechanism contributing to hereditary thrombophilia
    N H Gehring
    Department of Pediatrics, , Humboldt University, Berlin, Germany
    Nat Genet 28:389-92. 2001
    The G-->A mutation at position 20210 of the prothrombin or coagulation factor II gene (F2) represents a common genetic risk factor for the occurrence of thromboembolic events...
  11. ncbi Early decrease in α-fetoprotein, but not des-γ-carboxy prothrombin, predicts sorafenib efficacy in patients with advanced hepatocellular carcinoma
    Teiji Kuzuya
    Division of Gastroenterology and Hepatology, Musashino Red Cross Hospital, Musashino, Tokyo, Japan
    Oncology 81:251-8. 2011
    ..the relationships between early changes in the tumor markers α-fetoprotein (AFP) and des-γ-carboxy prothrombin (DCP), and antitumor response in the early period following administration of sorafenib in patients with ..
  12. pmc Alpha-fetoprotein, des-gamma carboxyprothrombin, and lectin-bound alpha-fetoprotein in early hepatocellular carcinoma
    Jorge A Marrero
    University of Michigan, Ann Arbor, Michigan 48109, USA
    Gastroenterology 137:110-8. 2009
    ..Des-gamma carboxy-prothrombin (DCP) and lectin-bound AFP (AFP-L3%) are potential surveillance tests for HCC...
  13. pmc In vivo detection of Staphylococcus aureus endocarditis by targeting pathogen-specific prothrombin activation
    Peter Panizzi
    Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Nat Med 17:1142-6. 2011
    ..Here we report that an engineered analog of prothrombin could be used to detect S...
  14. ncbi Decreased inhibitory activity of prothrombin to calcium oxalate crystallization by specific chemical modification of its gamma-carboxyglutamic acid residues
    Jihong Liu
    Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    Urology 67:201-3. 2006
    To investigate the inhibitory role of gamma (gamma)-carboxyglutamic acid (Gla) in the Gla domain of urinary prothrombin in the formation of calcium oxalate crystals.
  15. pmc Des-gamma-carboxy prothrombin as an important prognostic indicator in patients with small hepatocellular carcinoma
    Kenichi Hakamada
    Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine, 5 Zaifu cho, Hirosaki 036 8562, Japan
    World J Gastroenterol 14:1370-7. 2008
    To clarify the effect of a high des-gamma-carboxy prothrombin (DCP) level on the invasiveness and prognosis of small hepatocellular carcinoma.
  16. pmc Simultaneous activation of complement and coagulation by MBL-associated serine protease 2
    Anders Krarup
    MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, Oxford, United Kingdom
    PLoS ONE 2:e623. 2007
    ..We have shown that MASP2 is capable of promoting fibrinogen turnover by cleavage of prothrombin, generating thrombin...
  17. ncbi Prevalence of angiotensin-converting enzyme, methylenetetrahydrofolate reductase, Factor V Leiden, prothrombin and apolipoprotein E gene polymorphisms in Morocco
    Thierry Paluku They-They
    Laboratoire de Génétique Médicale et Pathologie Moléculaire LGPM, Faculté de Médecine et de Pharmacie Casablanca, 19 rue Tarik Ibn Ziad, Casablanca, Morocco
    Ann Hum Biol 37:767-77. 2010
    ..These genetic factors are involved in several pathways affecting blood pressure regulation, blood coagulation, homocysteine and lipid metabolisms...
  18. ncbi Varied prevalence of factor V G1691A (Leiden) and prothrombin G20210A single nucleotide polymorphisms among Arabs
    Wassim Y Almawi
    Al Jawhara Center for Molecular Medicine, Genetics and Inherited Diseases, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Bahrain
    J Thromb Thrombolysis 20:163-8. 2005
    Factor V G1691A (FV-Leiden) and prothrombin (PRT) G20210A single nucleotide polymorphisms (SNPs) are major inherited risk factors of venous thromboembolism...
  19. ncbi Prognostic and diagnostic value of des-γ-carboxy prothrombin in liver cancer
    Gaetano Bertino
    Department of Internal Medicine and Systemic Diseases, Santa Marta Hospital, Catania University, Catania, Italy
    Drug News Perspect 23:498-508. 2010
    Des-γ-carboxy prothrombin (DCP) is an abnormal prothrombin induced by the absence of vitamin K₂ that is increased in the serum of patients with hepatocellular carcinoma (HCC)...
  20. ncbi Des-gamma-carboxyprothrombin, alpha-fetoprotein and AFP-L3 in patients with chronic hepatitis, cirrhosis and hepatocellular carcinoma
    Francisco A Durazo
    The Pfleger Liver Institute, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    J Gastroenterol Hepatol 23:1541-8. 2008
    ....
  21. ncbi Des-gamma carboxyprothrombin can differentiate hepatocellular carcinoma from nonmalignant chronic liver disease in american patients
    Jorge A Marrero
    Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor 48109, USA
    Hepatology 37:1114-21. 2003
    ..Des-gamma carboxy-prothrombin (DCP) has been reported to be more sensitive and specific for the diagnosis of HCC in Japanese patients ..
  22. ncbi The direct thrombin inhibitor hirudin
    Andreas Greinacher
    Institute for Immunology and Transfusion Medicine, Ernst Moritz Arndt University Greifswald, Sauerbruchstrasse, 17489 Greifswald, Germany
    Thromb Haemost 99:819-29. 2008
    ..Drawbacks of hirudin and important precautions for avoiding potential adverse effects are discussed in detail in the third part of this review...
  23. ncbi Utility of Lens culinaris agglutinin-reactive fraction of alpha-fetoprotein and des-gamma-carboxy prothrombin, alone or in combination, as biomarkers for hepatocellular carcinoma
    Richard K Sterling
    Virginia Commonwealth University Health Systems, Richmond, Virginia, USA
    Clin Gastroenterol Hepatol 7:104-13. 2009
    Des-gamma-carboxy prothrombin (DCP) and Lens culinaris agglutinin-reactive fraction of alpha-fetoprotein (AFP-L3) are surveillance markers used to detect hepatocellular carcinoma (HCC) in Japan...
  24. ncbi The intronic prothrombin 19911A>G polymorphism influences splicing efficiency and modulates effects of the 20210G>A polymorphism on mRNA amount and expression in a stable reporter gene assay system
    Nicolas von Ahsen
    Georg August University, Department of Clinical Chemistry, Robert Koch Str 40, 37075 Gottingen, Germany
    Blood 103:586-93. 2004
    The common prothrombin gene cleavage site mutation 20210G>A is associated with elevated prothrombin levels and thrombosis...
  25. ncbi The effects of phosphatidylserine-dependent antiprothrombin antibody on thrombin generation
    Yoshie Sakai
    Department of Medicine II, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Arthritis Rheum 60:2457-67. 2009
    Antibodies to prothrombin (APTs) and to beta2-glycoprotein I are the major autoantibodies responsible for lupus anticoagulant (LAC) activity...
  26. ncbi Prognostic values of alpha-fetoprotein and protein induced by vitamin K absence or antagonist-II in hepatitis B virus-related hepatocellular carcinoma: a prospective study
    Hyoung Su Kim
    Department of Internal Medicine, Kangdong Sacred Heart Hospital of Hallym University Medical Center, Seoul, Republic of Korea
    J Clin Gastroenterol 43:482-8. 2009
    ..However, prognostic values of AFP and PIVKA-II remain clarified in hepatitis B virus (HBV)-associated HCC. This study was aimed to evaluate the prognostic values of AFP and PIVKA-II in HBV-associated HCC...
  27. ncbi In vitro assessment, using thrombin generation, of the applicability of prothrombin complex concentrate as an antidote for Rivaroxaban
    J Dinkelaar
    Haematology and Clinical Chemistry Laboratory, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands
    J Thromb Haemost 11:1111-8. 2013
    ..At present no antidote is appointed and no guidelines have been formulated for the measurement of Rivaroxaban reversal...
  28. ncbi High serum des-gamma-carboxy prothrombin level predicts poor prognosis after radiofrequency ablation of hepatocellular carcinoma
    Masahiro Kobayashi
    Department of Hepatology, Toranomon Hospital, Tokyo, Japan
    Cancer 115:571-80. 2009
    ..Radiofrequency ablation (RFA) has been an alternative choice for unresectable HCC. However, RFA is expected to have similar therapeutic efficacy for early stage HCC with fewer invasions...
  29. ncbi Plasma levels of prothrombin fragment F1+2, D-dimer and prothrombin time correlate with clinical stage and lymph node metastasis in operable gastric cancer patients
    Hyuk Chan Kwon
    Department of Internal Medicine, Dong A University College of Medicine, 3 1 Dongdaeshin dong, Seo Gu, Busan 602 715, South Korea
    Jpn J Clin Oncol 38:2-7. 2008
    ..The principal objective of this study was to determine the relationship between preoperative coagulation tests and the extent of tumor involvement in gastric cancer patients...
  30. ncbi Increase of prothrombin-mRNA after global cerebral ischemia in rats, with constant expression of protease nexin-1 and protease-activated receptors
    M Riek-Burchardt
    Project Group Neuropharmacology, Leibniz Institute for Neurobiology, POB 1860, Brenneckestrasse 6, 39118 Magdeburg, Germany
    Neurosci Lett 329:181-4. 2002
    b>Prothrombin, protease-activated receptors (PARs) and the specific thrombin inhibitor protease nexin-1 (PN-1) are expressed in the brain...
  31. ncbi Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains
    Jeanne Claíne de Albuquerque Modesto
    Laboratorio de Bioquimica e Biofisica, Instituto Butantan, Av Vital Brazil 1500, 05503 900 Sao Paulo, SP, Brazil
    Biol Chem 386:589-600. 2005
    The first low-molecular-mass metalloprotease presenting prothrombin activating activity was purified from Bothrops insularis venom and named insularinase A. It is a single-chain protease with a molecular mass of 22 639 Da...
  32. ncbi Frequency of factor V, prothrombin and methylenetetrahydrofolate reductase gene variants in preeclampsia
    Angela V D'Elia
    Department of Biomedical Science and Technology, University of Udine, Udine, Italy
    Gynecol Obstet Invest 53:84-7. 2002
    The association between thrombophilic variants (Leiden mutation of the factor V gene, G20210A mutation of the prothrombin gene and C677T polymorphism of the methylenetetrahydrofolate reductase (MTHFR) gene) with preeclampsia was ..
  33. ncbi Cooperative roles of factor V(a) and phosphatidylserine-containing membranes as cofactors in prothrombin activation
    Gabriel E Weinreb
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 278:5679-84. 2003
    Activation of prothrombin, as catalyzed by the prothrombinase complex (factor X(a), enzyme; factor V(a) and phosphatidylserine (PS)-containing membranes, cofactors), involves production and subsequent proteolysis of two possible ..
  34. ncbi The presence of anti-phosphatidylserine/prothrombin antibodies as risk factor for both arterial and venous thrombosis in patients with systemic lupus erythematosus
    Junzo Nojima
    Laboratory for Clinical Investigation, Osaka University Hospital, Suita, Osaka 565 0871, Japan
    Haematologica 91:699-702. 2006
    ..I antibodies (anti-beta2-GPI), antiprothrombin antibodies (anti-PT), and anti-phosphatidylserine/prothrombin antibodies (anti-PS/PT) in 175 patients with systemic lupus erythematosus (SLE) comprising 67 patients with ..
  35. ncbi Effect of prothrombin and its activation fragments on calcium oxalate crystal growth and aggregation in undiluted human urine in vitro: relationship between protein structure and inhibitory activity
    Phulwinder K Grover
    Urology Unit, Department of Surgery, Flinders Medical Centre, Bedford Park, South Australia 5042, Australia
    Clin Sci (Lond) 102:425-34. 2002
    In recent years there has been great interest in the putative role of prothrombin and its activation peptides, especially the urinary form of prothrombin fragment 1, in the pathogenesis of calcium oxalate (CaOx) urolithiasis...
  36. ncbi Comparison of clinicopathological features of patients with hepatocellular carcinoma seropositive for alpha-fetoprotein alone and those seropositive for des-gamma-carboxy prothrombin alone
    H Okuda
    Institute of Gastroenterology, Tokyo Women s Medical University, Tokyo, Japan
    J Gastroenterol Hepatol 16:1290-6. 2001
    ..of the clinicopathological features of HCC patients who are seropositive for alpha-fetoprotein (AFP) alone and those who are seropositive for des-gamma-carboxy prothrombin (DCP) alone. The authors, thus, performed this comparative study.
  37. ncbi Tumor markers in early diagnosis, follow-up and management of patients with hepatocellular carcinoma
    Shigetoshi Fujiyama
    Third Department of Internal Medicine, Kumamoto University School of Medicine, Japan
    Oncology 62:57-63. 2002
    ..Equally important in the management of patients with HCC are biological indicators for malignancy, the selection of therapeutic interventions and the prediction of the outcome...
  38. pmc Significance of antiprothrombin antibodies in patients with systemic lupus erythematosus: clinical evaluation of the antiprothrombin assay and the antiphosphatidylserine/prothrombin assay, and comparison with other antiphospholipid antibody assays
    Akito Tsutsumi
    Division of Clinical Immunology, Major of Advanced Biomedical Applications, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, 305 8575, Japan
    Mod Rheumatol 16:158-64. 2006
    Antibodies against prothrombin are detected by enzyme immunoassays (EIA) in sera of patients with antiphospholipid syndrome (APS)...
  39. ncbi Clinical evaluation of lens culinaris agglutinin-reactive alpha-fetoprotein and des-gamma-carboxy prothrombin in histologically proven hepatocellular carcinoma in the United States
    Brian I Carr
    Liver Cancer Center, Starzl Transplantation Institute, University of Pittsburgh Medical Center, E1552 BST, 200 Lothrop Street, Pittsburgh, Pennsylvania 15213, USA
    Dig Dis Sci 52:776-82. 2007
    ..role for the lens culinaris agglutinin-reactive fraction of alpha-fetoprotein (AFP-L3%) and des-gamma-carboxy prothrombin (DCP) in the management of the U.S. hepatocellular carcinoma (HCC) patient population...
  40. pmc Serum tumor markers for detection of hepatocellular carcinoma
    Lin Zhou
    Division of Biotherapy for Cancer, Cancer Center, West China Hospital of Sichuan University, 37 Guoxue Xiang, Chengdu 610041, Sichuan Province, China
    World J Gastroenterol 12:1175-81. 2006
    ....
  41. pmc A potential regulatory role for mRNA secondary structures within the prothrombin 3'UTR
    Xingge Liu
    Department of Medicine Hematology Oncology, University of Pennsylvania School of Medicine and The Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
    Thromb Res 126:130-6. 2010
    The distal 3'UTR of prothrombin mRNA exhibits significant sequence heterogeneity reflecting an inexact 3'-cleavage/polyadenylation reaction...
  42. ncbi Geographic distribution of the 20210 G to A prothrombin variant
    F R Rosendaal
    Department of Clinical Epidemiology, Leiden University Medical Centre, The Netherlands
    Thromb Haemost 79:706-8. 1998
    A variant in prothrombin (clotting factor II), a G to A transition at nucleotide position 20210, has recently been shown to be associated with the prothrombin plasma levels and the risk of both venous and arterial thrombosis...
  43. ncbi Early increase in α-fetoprotein for predicting unfavorable clinical outcomes in patients with advanced hepatocellular carcinoma treated with sorafenib
    Takahide Nakazawa
    Department of Gastroenterology, Internal Medicine, Kitasato University School of Medicine, Tokyo, Japan
    Eur J Gastroenterol Hepatol 25:683-9. 2013
    To determine the value of early alterations of the tumor markers α-fetoprotein (AFP) and des-γ-carboxy prothrombin (DCP) for predicting the outcomes of patients with advanced hepatocellular carcinoma (HCC) who receive sorafenib.
  44. ncbi Early on-treatment predictions of clinical outcomes using alpha-fetoprotein and des-gamma-carboxy prothrombin responses in patients with advanced hepatocellular carcinoma
    Myoung Ha Lee
    Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea
    J Gastroenterol Hepatol 27:313-22. 2012
    The clinical utility of alpha-fetoprotein (AFP) and des-γ-carboxy prothrombin (DCP) as a predictor of treatment outcome in patients with advanced hepatocellular carcinoma (HCC) receiving hepatic artery infusional chemotherapy (HAIC) or ..
  45. ncbi Clinical and molecular insights into the hepatocellular carcinoma tumour marker des-γ-carboxyprothrombin
    Yoshinori Inagaki
    Hepato Biliary Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
    Liver Int 31:22-35. 2011
    ..However, various issues have to be resolved to construct a novel therapy for HCC-targeting DCP. Innovation is required to make further progress in examining DCP...
  46. ncbi Inhibition of calcium oxalate crystal growth and aggregation by prothrombin and its fragments in vitro: relationship between protein structure and inhibitory activity
    P K Grover
    Urology Unit, Department of Surgery, Flinders Medical Centre, Flinders University of South Australia
    Eur J Biochem 263:50-6. 1999
    During blood coagulation, prothrombin (PT) is ultimately degraded to three fragments, thrombin, fragment 1 (F1) and fragment 2 (F2), which, collectively, contain all of the structural features of PT...
  47. ncbi The significance of genetic polymorphisms of factor V Leiden and prothrombin in the preeclamptic Polish women
    Agnieszka Seremak-Mrozikiewicz
    Division of Perinatology and Women s Diseases, Department of Perinatology and Gynecology, Poznan University of Medical Sciences, Poznan, Poland
    J Thromb Thrombolysis 30:97-104. 2010
    ..Inherited thrombophilias, like polymorphism of factor V (FV) Leiden and prothrombin (PTM) are considered to be involved in the PE development...
  48. ncbi Diagnostic and prognostic value of alpha-fetoprotein, des-γ-carboxy prothrombin and squamous cell carcinoma antigen immunoglobulin M complexes in hepatocellular carcinoma
    G Bertino
    Department of Internal Medicine and Systemic Diseases, Catania University, Catania, Italy
    Minerva Med 102:363-71. 2011
    ..The aim of this study was compare the accuracy of α-fetoprotein (AFP), des-γ- carboxy prothrombin (DCP), squamous cell carcinoma antigen-immunoglobulin M complexes (SCCA-IgM Cs) in the early diagnosis and in ..
  49. ncbi Linkage analysis demonstrates that the prothrombin G20210A mutation jointly influences plasma prothrombin levels and risk of thrombosis
    J M Soria
    Unitat d Hemostàsia i Trombosi Hospital de la Santa Creu i Sant Pau, Barcelona, Spain
    Blood 95:2780-5. 2000
    Association studies suggest that the G20210A mutation (G to A substitution at nucleotide position 20210) in the prothrombin gene (PT) is associated with increased plasma prothrombin activity and with increased risk for venous ..
  50. ncbi Combined measurement of preoperative α-fetoprotein and des-γ-carboxy prothrombin predicts recurrence after curative resection in patients with hepatitis-B-related hepatocellular carcinoma
    Young Eun Chon
    Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea
    Int J Cancer 131:2332-41. 2012
    Alpha-fetoprotein (AFP) and des-γ-carboxy prothrombin (DCP) are widely used complementary tumor markers for hepatocellular carcinoma (HCC)...
  51. ncbi Evaluation of prothrombin complex concentrate and recombinant activated factor VII to reverse rivaroxaban in a rabbit model
    Anne Godier
    Department of Anaesthesia and Critical Care, Paris Descartes University, Hotel Dieu University Hospital, Paris, France
    Anesthesiology 116:94-102. 2012
    ..Objectives were to study efficacy and safety of recombinant activated factor VII (rFVIIa) and prothrombin complex concentrate (PCC) to reverse the anticoagulant effect of an overdose of rivaroxaban in a rabbit model ..
  52. ncbi Clinical characteristics and prognosis of hepatocellular carcinoma with different sets of serum AFP and PIVKA-II levels
    Se Hun Kang
    Department of Internal Medicine, Yonsei University College, Seoul, Korea
    Eur J Gastroenterol Hepatol 24:849-56. 2012
    Fetoprotein (AFP) and prothrombin induced by the absence of vitamin K or antagonist-II (PIVKA-II) are useful tumor markers for hepatocellular carcinoma (HCC)...
  53. ncbi Prevalence of antibodies to prothrombin in solid phase (aPT) and to phosphatidylserine-prothrombin complex (aPS/PT) in patients with and without lupus anticoagulant
    Maria Laura Bertolaccini
    Lupus Research Unit, The Rayne Institute, Division of Women s Health, King s College London, 4th Floor Lambeth Wing, St Thomas Hospital, London SE1 7EH, UK
    Thromb Haemost 109:207-13. 2013
    Antibodies to prothrombin in solid phase (aPT) and those to phosphatidiyserine-prothrombin complex (aPS/PT) have been suggested to strongly correlate with the presence of lupus anticoagulant (LA)...
  54. ncbi Characterization of the protein Z-dependent protease inhibitor
    X Han
    Division of Hematology, Barnes Jewish Hospital at Washington University Medical Center, St Louis, MO 63110, USA
    Blood 96:3049-55. 2000
    ..dramatically delays the initiation and reduces the ultimate rate of thrombin generation in mixtures containing prothrombin, factor V, phospholipids, and Ca(++)...
  55. pmc The association of factor V leiden and prothrombin gene mutation and placenta-mediated pregnancy complications: a systematic review and meta-analysis of prospective cohort studies
    Marc A Rodger
    Thrombosis Program, Division of Hematology, Departments of Medicine, Obstetrics and Gynecology and Epidemiology Community Medicine, University of Ottawa, Ottawa, Ontario, Canada
    PLoS Med 7:e1000292. 2010
    Factor V Leiden (FVL) and prothrombin gene mutation (PGM) are common inherited thrombophilias...
  56. ncbi Combined effect of factor V Leiden and prothrombin 20210A on the risk of venous thromboembolism--pooled analysis of 8 case-control studies including 2310 cases and 3204 controls. Study Group for Pooled-Analysis in Venous Thromboembolism
    J Emmerich
    Hopital Europeen Georges Pompidou, Service de Medecine Vasculaire, Centre Claude Bernard et Laboratoire d Hémostase, Paris, France
    Thromb Haemost 86:809-16. 2001
    ..69). Conversely, factor II G20210A mutation was equally balanced in both patient groups...
  57. ncbi Glycosylation of prothrombin fragment 1 governs calcium oxalate crystal nucleation and aggregation, but not crystal growth
    Dawn Webber
    Department of Chemistry, University of Cape Town, Private Bag, Rondebosch, Cape Town, 7701, South Africa
    Urol Res 35:277-85. 2007
    ..In several cases, this has been attributed to glycosylation of the proteins as evidenced by urinary prothrombin fragment 1 where there is a correlation between sialylation and calcium oxalate kidney stone disease...
  58. ncbi Integrin alpha5-induced EGFR activation by prothrombin triggers hepatocyte apoptosis via the JNK signaling pathway
    Ziqiu Wang
    Department of Medical Oncology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    J Cell Physiol 216:551-7. 2008
    We have previously shown that prothrombin, a blood coagulation factor, can cause an inhibition of DNA synthesis in normal rat hepatocytes...
  59. ncbi EPCR Ser219Gly: elevated sEPCR, prothrombin F1+2, risk for coronary heart disease, and increased sEPCR shedding in vitro
    H Ireland
    Cardiovascular Genetics, Department Medicine, University College London, 5 University St, London, WC1E 6JF, UK
    Atherosclerosis 183:283-92. 2005
    ..Furthermore, in base-line samples from NPHSII and in the diabetic study, a significant increase in prothrombin F1+2 level was observed for 219Gly...
  60. ncbi Effects of Mucuna pruriens extract on activation of prothrombin by Echis carinatus venom
    R Guerranti
    Institute of Biochemistry and Enzymology, University of Siena, Via A Moro, 53100, Siena, Italy
    J Ethnopharmacol 75:175-80. 2001
    ..Here the effect of an extract of MP101UJ in prothrombin activation by EV in vitro by clotting and chromogenic assay is studied...
  61. ncbi Plasma levels of plasminogen activator inhibitor type 1, factor VIII, prothrombin activation fragment 1+2, anticardiolipin, and antiprothrombin antibodies are risk factors for thrombosis in hemodialysis patients
    Daniela Molino
    Divison of Nephrology, Second University of Naples, Naples, Italy
    Semin Nephrol 24:495-501. 2004
    ..All patients underwent a screening prothrombin time, activated partial thromboplastin time, fibrinogen (Fg), coagulation factors of the intrinsic and ..
  62. ncbi Renal prothrombin mRNA is significantly decreased in a hyperoxaluric rat model of nephrolithiasis
    P K Grover
    Urology Unit, Department of Surgery, Flinders Medical Centre and Flinders University Bedford Park 5042, South Australia, Australia
    J Pathol 210:273-81. 2006
    Although urinary prothrombin fragment 1 (UPTF1) possesses several hallmarks expected of a regulatory protein in urolithiasis, its precise role remains unknown...
  63. ncbi Fibrin presence within aortic valves in patients with aortic stenosis: association with in vivo thrombin generation and fibrin clot properties
    Joanna Natorska
    Institute of Cardiology, Jagiellonian University School of Medicine, 80 Pradnicka St, 31 202 Krakow, Poland
    Thromb Haemost 105:254-60. 2011
    ..001). Patients with AS had elevated plasma D-dimer (236.4 ± 28 ng/ml, p=0.002) and prothrombin fragment 1+2 (F1.2) (261.7 ± 27.1 pM, p=0.005) compared to AI subjects (142.8 ± 10 ng/ml and 131.2 ± 1...
  64. ncbi The limits of simulation of the clotting system
    R Wagenvoord
    Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, Netherlands
    J Thromb Haemost 4:1331-8. 2006
    ..To investigate in how far successful simulation of a thrombin generation (TG) curve gives information about the underlying biochemical reaction mechanism...
  65. ncbi Dengue virus nonstructural protein NS1 binds to prothrombin/thrombin and inhibits prothrombin activation
    Shi Wei Lin
    Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan, ROC
    J Infect 64:325-34. 2012
    ..Since thrombin plays a very important role in the activation of coagulation, we investigated whether NS1 can bind to thrombin and affect its formation or activity...
  66. ncbi Use of serum PIVKA-II (DCP) determination for differentiation between benign and malignant liver diseases
    R Lamerz
    Department of Internal Medicine II, Ludwig Maximilians University, Munich, Germany
    Anticancer Res 19:2489-93. 1999
    The concentration of Des-gamma-carboxy-prothrombin (DCP) or PIVKA-II has been described to be increased in patients with hepatocellular cancer, along with its elevation in vitamin K deficient states by warfarin or dicoumarol treatment...
  67. ncbi Time-dependent analysis of predisposing factors for the recurrence of hepatocellular carcinoma
    Shouta Iwadou
    Department of Internal Medicine, Hiroshima City Hospital, Hiroshima, Japan
    Liver Int 30:1027-32. 2010
    ..The aim of this study is to evaluate the predisposing factors after treatments...
  68. ncbi Severe venous thromboembolism in a young man with Klinefelter's syndrome and heterozygosis for both G20210A prothrombin and factor V Leiden mutations
    Mario Lapecorella
    Centro Emostasi e Trombosi, Dipartimento di Clinica Medica, Immunologia e Malattie Infettive MIDIM, University of Bari School of Medicine, Italy
    Blood Coagul Fibrinolysis 14:95-8. 2003
    ..Further examinations also showed a double heterozygosis for G20210A prothrombin and factor V Leiden mutations...
  69. ncbi Inhibition of growth and aggregation of calcium oxalate crystals in vitro--a comparison of four human proteins
    P K Grover
    Department of Surgery, Flinders Medical Centre, Adelaide, South Australia, Australia
    Eur J Biochem 253:637-44. 1998
    ..the inhibitory effects of Tamm-Horsfall glycoprotein (THG), human serum albumin (HSA), alpha1-microglobulin and prothrombin fragment 1 (PTF1) on calcium oxalate crystallization...
  70. ncbi Prothrombin index decrease: a useful and reliable marker of extensive fibrosis?
    Jean Francois Cadranel
    Unité d Hépatologie, Hopital Laennec, BP 72, 60109 Creil Cedex, France
    Eur J Gastroenterol Hepatol 14:1057-9. 2002
    ..Among those serum markers, we focused on prothrombin index, which seems to be a reliable and non-expansive marker for the diagnosis of extensive fibrosis.
  71. ncbi Prevalence of factor V Leiden and prothrombin G20210A mutation in a large French population selected for nonthrombotic history: geographical and age distribution
    Elisabeth Mazoyer
    Hématologie biologique, Hopital Lariboisiere, Paris, France
    Blood Coagul Fibrinolysis 20:503-10. 2009
    ..Thirty percent of variation in plasma prothrombin level was explained by environmental factors (serum cholesterol, age, oral contraception, hormonal replacement ..
  72. ncbi Prevalence of antiphospholipid antibodies, factor V G1691A (Leiden) and prothrombin G20210A mutations in early and late recurrent pregnancy loss
    Nabil Mtiraoui
    Research Unit of Haematological and Autoimmune Diseases, Faculty of Pharmacy, Monastir, Center University, Tunisia
    Eur J Obstet Gynecol Reprod Biol 119:164-70. 2005
    ..We assessed the prevalence of inherited (FV-Leiden and PRT G20210A), and acquired (anti-PL antibodies) risk factors among habitual aborters in Tunisia...
  73. ncbi Factor V Leiden G1691A and prothrombin G20210A mutations are common in Tunisia
    C Frere
    J Thromb Haemost 1:2451-2. 2003
  74. ncbi Factor V Leiden and prothrombin gene G20210A mutations are uncommon in portal vein thrombosis in India
    Sanjay Sharma
    Department of Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow 226 014, India
    Indian J Gastroenterol 25:236-9. 2006
    ..The cause of thrombosis in these patients remains unclear. We studied the frequency of mutations in genes for coagulation factors V and II (prothrombin) in 61 Indian patients with PVT and 49 healthy control subjects.
  75. ncbi Prevalence of two thrombophilia predisposing mutations: factor V G1691A (R506Q; Leiden) and prothrombin G20210A, among healthy Lebanese
    Hala Tamim
    Thromb Haemost 88:691-2. 2002
  76. ncbi Prevalence of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations in 200 healthy Jordanians
    Suhair S Eid
    Molecular Laboratory, King Hussein Medical Centre, Jordan
    Clin Lab Sci 17:200-2. 2004
    ..In order to estimate the frequency of the factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations in the Jordanian population, we screened 200 healthy Jordanian individuals...
  77. ncbi Prevalence of factor V Leiden, prothrombin and methylene tetrahydrofolate reductase mutations in women with adverse pregnancy outcomes in Lebanon
    Laila F Zahed
    Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Lebanon
    Am J Obstet Gynecol 195:1114-8. 2006
    The purpose of this study was to determine the prevalence of factor V Leiden, prothrombin, and methylene tetrahydrofolate reductase gene mutations in women with adverse pregnancy outcome compared with women who had uneventful pregnancies.
  78. ncbi Impact of infusion speed on the safety and effectiveness of prothrombin complex concentrate: a prospective clinical trial of emergency anticoagulation reversal
    Ingrid Pabinger
    Department of Internal Medicine, Division of Haematology and Haemostaseology, Medical University Vienna, Vienna, Austria
    Ann Hematol 89:309-16. 2010
    b>Prothrombin complex concentrate (PCC) infusion is preferred for emergency reversal of coumarin therapy...
  79. ncbi High incidence of factor V Leiden and prothrombin G20210A in healthy southern Italians
    Gianluca Sottilotta
    Centro Emofilia, Servizio Emostasi e Trombosi, Azienda Ospedaliera Bianchi Melacrino Morelli, Reggio Calabria, Italy
    Clin Appl Thromb Hemost 15:356-9. 2009
    Factor V Leiden (FVL) and G20210 prothrombin (FII G20210A) mutations are risk factors for thromboembolism...
  80. ncbi Thrombogenicity of prothrombin complex concentrates
    M Kohler
    Department of Transfusion Medicine, University of Gottingen, Germany
    Thromb Res 95:S13-7. 1999
    Thrombogenicity of factor IX complex or prothrombin complex concentrates (PCC) is a well-acknowledged problem...
  81. ncbi Prevalence and association of the factor V Leiden and prothrombin G20210A in healthy subjects and patients with venous thromboembolism
    D Coen
    Clinical Institute of Laboratory Diagnostics, Zagreb University Hospital Center, Zagreb, Croatia
    Croat Med J 42:488-92. 2001
    To determine the prevalences of factor V Leiden and the G20210A mutation in the prothrombin gene (PT20210A) and the frequency of their association in healthy subjects and in patients with venous thromboembolism (VTE).
  82. ncbi Absence of factor V Leiden mutation and low prothrombin G 20210 A mutation prevalence in a healthy Moroccan population
    Florence Mathonnet
    Thromb Haemost 88:1073-4. 2002
  83. ncbi Risk assessment for recurrent venous thrombosis
    Paul Alexander Kyrle
    Department of Medicine I, Medical University of Vienna, Vienna, Austria
    Lancet 376:2032-9. 2010
    ..Analysis of preliminary data suggests that risk assessment can also be refined through integration of prothrombotic coagulation changes and clinical risk factors...
  84. ncbi Thrombin generation in acute coronary syndrome and stable coronary artery disease: dependence on plasma factor composition
    K Brummel-Ziedins
    Department of Biochemistry, University of Vermont, VT 05446, USA
    J Thromb Haemost 6:104-10. 2008
    ..We investigated whether thrombin generation based on circulating coagulation protein levels, could distinguish between acute and stable coronary artery disease (CAD)...
  85. ncbi The prothrombin 20209 C-->T mutation in Jewish-Moroccan Caucasians: molecular analysis of gain-of-function of 3' end processing
    S Danckwardt
    Departments of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
    J Thromb Haemost 4:1078-85. 2006
    Mutations of the 3' end mRNA-processing signal of the prothrombin (F2) gene have been reported to cause elevated F2 plasma concentrations, thrombosis, and complications of pregnancy...
  86. pmc Genetic elimination of prothrombin in adult mice is not compatible with survival and results in spontaneous hemorrhagic events in both heart and brain
    Eric S Mullins
    Division of Pediatric Hematology Oncology, Cincinnati Children s Hospital Research Foundation and University of Cincinnati College of Medicine, OH 45229 3039, USA
    Blood 113:696-704. 2009
    Mice carrying a conditional prothrombin knockout allele (fII(lox)) were established to develop an experimental setting for exploring the importance of thrombin in the maintenance of vascular integrity, the inflammatory response, and ..
  87. ncbi Factor V Leiden is associated with pre-eclampsia but not with fetal growth restriction: a genetic association study and meta-analysis
    T Dudding
    Hunter Genetics, Hunter New England Health Service, NSW, Australia
    J Thromb Haemost 6:1869-75. 2008
    ..factor V gene, the 1691G to A change (rs6025) located on chromosome 1q23 (factor V Leiden, FVL), and the other in the prothrombin gene, 20210G to A change (rs1799963) on chromosome 11p11-q12 (PT). These results, however, are conflicting.
  88. ncbi Malignancies, prothrombotic mutations, and the risk of venous thrombosis
    Jeanet W Blom
    Department of Clinical Epidemiology, Leiden University Medical Center, Leiden, The Netherlands
    JAMA 293:715-22. 2005
    ..Venous thrombosis is a common complication in patients with cancer, leading to additional morbidity and compromising quality of life...
  89. ncbi Staphylocoagulase is a prototype for the mechanism of cofactor-induced zymogen activation
    Rainer Friedrich
    Abteilung Strukturforschung, Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    Nature 425:535-9. 2003
    ..human pathogen implicated in sepsis and endocarditis, secretes the cofactor staphylocoagulase, which activates prothrombin, without the usual proteolytic cleavages, to directly initiate blood clotting. Here we present the 2...
  90. ncbi Lipoprotein (a) and genetic polymorphisms of clotting factor V, prothrombin, and methylenetetrahydrofolate reductase are risk factors of spontaneous ischemic stroke in childhood
    U Nowak-Gottl
    Department of Paediatrics, Institute of Clinical Chemistry and Laboratory Medicine and Institute of Arteriosclerosis Research, Westphalian Wilhelms University Münster, Germany
    Blood 94:3678-82. 1999
    ..lipoprotein (a) [Lp(a)] levels >30 mg/dL; for the presence of the factor V (FV) G1691A mutation, the prothrombin (PT) G20210A variant, and the TT677 genotype of methylenetetrahydrofolate reductase (MTHFR); and deficiencies ..
  91. ncbi Exosite-driven substrate specificity and function in coagulation
    S Krishnaswamy
    Joseph Stokes Research Institute, Children s Hospital of Philadelphia and Department of Pediatrics, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Thromb Haemost 3:54-67. 2005
    ..Exosite-driven enzymic function probably represents a widely employed biological strategy for the achievement of high macromolecular substrate specificity...
  92. ncbi The predictors of microvascular invasion in candidates for liver transplantation with hepatocellular carcinoma-with special reference to the serum levels of des-gamma-carboxy prothrombin
    Ken Shirabe
    Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    J Surg Oncol 95:235-40. 2007
    ..The size and histological grade of the tumor were significantly correlated with the mvi. The des-gamma-carboxy prothrombin (DCP) is a mvi predictor...
  93. ncbi The prothrombin 3'end formation signal reveals a unique architecture that is sensitive to thrombophilic gain-of-function mutations
    Sven Danckwardt
    Molecular Medicine Partnership Unit, Im Neuenheimer Feld 153, 69120 Heidelberg, Germany
    Blood 104:428-35. 2004
    The functional analysis of the common prothrombin 20210 G>A(F2 20210(*)A) mutation has recently revealed gain of function of 3'end processing as a novel genetic mechanism predisposing to human disease...
  94. ncbi Significance of des-gamma-carboxy prothrombin in selection criteria for living donor liver transplantation for hepatocellular carcinoma
    M Fujiki
    Department of Transplant and General Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan
    Am J Transplant 9:2362-71. 2009
    Des-gamma-carboxy prothrombin (DCP) levels reportedly correlate with histological features of hepatocellular carcinoma (HCC)...
  95. ncbi Risk of recurrent venous thromboembolism in patients with common thrombophilia: a systematic review
    Wai Khoon Ho
    Department of Haematology, Royal Perth Hospital and Centre for Clinical Research Excellence, University of Western Australia, Perth
    Arch Intern Med 166:729-36. 2006
    ..polymorphisms that predispose to a first episode of venous thromboembolism (VTE) are factor V Leiden (FVL) and prothrombin G20210A. However, the effect of these polymorphisms on the risk of recurrent VTE is unclear...
  96. ncbi Generation of C5a in the absence of C3: a new complement activation pathway
    Markus Huber-Lang
    Department of Traumatology, Hand and Reconstructive Surgery, University of Ulm Medical School, Steinhoevelstrasse 9, D 89075 Ulm, Germany
    Nat Med 12:682-7. 2006
    ..There were higher levels of F2 mRNA (encoding prothrombin) as well as prothrombin and thrombin protein in liver of C3-/- mice compared to C3+/+ mice...
  97. ncbi Preparation of recombinant alpha-thrombin: high-level expression of recombinant human prethrombin-2 and its activation by recombinant ecarin
    Hiroshi Yonemura
    Applied Research Department, The Chemo Sero Therapeutic Research Institute Kaketsuken, Kawabe, Kyokushi, Kikuchi, Kumamoto 869 1298
    J Biochem 135:577-82. 2004
    ..Our system is suitable for the large-scale production of recombinant alpha-thrombin, which can be used in place of clinically available alpha-thrombin derived from human or bovine plasma...
  98. ncbi Antiprothrombin antibodies detected in two different assay systems. Prevalence and clinical significance in systemic lupus erythematosus
    Maria Laura Bertolaccini
    Lupus Research Unit, The Rayne Institute, St Thomas Hospital, London SE1 7EH, UK
    Thromb Haemost 93:289-97. 2005
    ..Results show that anti-prothrombin antibodies were found in 47% of the patients (aPT in 31% and aPS-PT in 31%)...
  99. ncbi The G20210A prothrombin-gene mutation and the plasminogen activator inhibitor (PAI-1) 5G/5G genotype are associated with early onset of severe preeclampsia
    A Gerhardt
    Department of Obstetrics and Gynecology, Heinrich Heine University Medical Center, Dusseldorf, Germany
    J Thromb Haemost 3:686-91. 2005
    ..9, 95% confidence interval (CI) 0.4, 2.2; prothrombin mutation, OR 1.9, 95% CI 0.5, 7.0; methylentetrahydrofolate reductase 677TT genotype, OR 0.8, 95% CI 0.4, 1...
  100. pmc Prothrombin gene G20210A mutation and obstetric complications
    Robert M Silver
    Departments of Obstetrics and Gynecology at the University of Alabama at Birmingham, Birmingham, Alabama, USA
    Obstet Gynecol 115:14-20. 2010
    To estimate whether maternal carriage of the prothrombin gene G20210A mutation is associated with pregnancy loss, preeclampsia, placental abruption, or small for gestational age (SGA) neonates in a low-risk, prospective cohort.
  101. ncbi Risk for subsequent venous thromboembolic complications in carriers of the prothrombin or the factor V gene mutation with a first episode of deep-vein thrombosis
    P Simioni
    Department of Medical and Surgical Sciences, Second Chair of Internal Medicine, University Hospital of Padua, Italy
    Blood 96:3329-33. 2000
    Carriers of a mutation in the prothrombin (clotting factor II) or factor V gene have a 2- to 4-fold greater risk for venous thromboembolism than subjects without the mutations...

Research Grants65

  1. Dual Direct Inhibitors of Thrombin and Factor Xa
    UMESH RAMANLAL DESAI; Fiscal Year: 2012
    ..1) Sulfated DHPs (CDs, FDs and SDs) prolong prothrombin time at concentrations 2-6-fold below that of the clinically used LMWH enoxaparin, while in the activated ..
  2. Intralipid: A novel frontline countermeasure for brodifacoum poisoning
    Douglas L Feinstein; Fiscal Year: 2013
    ..the body by inhibiting its recycling leading to loss of activity of Vitamin K dependent proteins including prothrombin. Loss of prothrombin leads to systemic anticoagulation, hence the basis for the widespread use of ..
  3. Mechanism of Staphylocoagulase-activated Blood Clotting
    Paul E Bock; Fiscal Year: 2013
    ..aureus bacteremia allows bacteria to bind to the injury site. SC/vWbp activate host prothrombin (ProT) non-proteolytically by inserting their N-termini into a cleft in ProT. Active SC/vWbp...
  4. Pathogen specific imaging of endocarditis
    PETER ROLF PANIZZI; Fiscal Year: 2013
    ..the ultrahigh affinity (17pM)5 of the virulence factor staphylocoagulase, which is secreted by the bacteria, to prothrombin (ProT) and the use of this interaction to develop specific imaging probes6...
  5. Integrating plasma IGF-1 into novel classification of hepatocellular carcinoma
    Ahmed Kaseb; Fiscal Year: 2013
    ..laboratory-based parameters that assess the synthetic function of the liver through serum albumin level and prothrombin time or the elimination function of the liver through measuring serum bilirubin level, in addition to two ..
  6. Comprehensive studies of novel SNPs affecting warfarin dose in African Americans
    Minoli Perera; Fiscal Year: 2012
    ..a pharmacodynamic study evaluating the effect of VKORC1 SNPs on a surrogate marker of VKOR protein function, Prothrombin induced by vitamin K absence or antagonism II (PIVKA-II)...
  7. Mechanisms linking the hemostatic protease thrombin to arthritic disease
    Matthew J Flick; Fiscal Year: 2013
    ..Activation of the hemostatic system and the subsequent conversion of prothrombin to the active serine protease thrombin is a prominent feature of RA...
  8. Insights on Selected Procoagulation Markers and Outcomes in Stroke Trial (I-SPOT)
    ANGARA KONETI RAO; Fiscal Year: 2013
    ..procoagulant activity (TF-PCA) and plasma activated factor VII (FVIIa) and markers of thrombin generation, prothrombin fragment 1.2 (F1.2) and thrombin- antithrombin complexes (TAT), are markedly elevated after AIS...
  9. Pathobiology of Staphyloccocus areus Endocarditis
    PETER ROLF PANIZZI; Fiscal Year: 2010
    ..S. aureus expresses a unique non-proteolytic prothrombin (ProT) activator, staphylocoagulase that mediates recognition of fibrinogen by the active staphylocoagulase-..
  10. The Activation of Prothrombin
    Kenneth G Mann; Fiscal Year: 2011
    ..abstract_text> ..
  11. Lipid Regulation of Thrombin Generation
    Barry R Lentz; Fiscal Year: 2012
    Thrombin, the central regulatory molecule of blood coagulation, is produced by prothrombin (II) proteolysis by a platelet-membrane-bound enzyme (factor Xa) and its associated cofactor (factor Va)...
  12. Pathobiology of Staphyloccocus areus Endocarditis
    PETER ROLF PANIZZI; Fiscal Year: 2013
    ..5. aureus expresses a unique non-proteolytic prothrombin (ProT) activator, staphylocoagulase that mediates recognition of fibrinogen by the active staphylocoagulase-..
  13. Hemostatic factors and sickle cell disease
    Punam Malik; Fiscal Year: 2013
    ..These hypotheses will be tested through studies that focus on defining the importance of prothrombin in the development of multi-organ SCD pathologies and long-term survival in Berkeley sickle mice (Hba0/Hbb0 [Tg(..
  14. Mechanism of Staphylocoagulase-activated Blood Clotting
    Paul E Bock; Fiscal Year: 2012
    ..are proposed to address major gaps in the understanding of the unique mechanism by which SC activates prothrombin (ProT) non-proteolytically and forms an SOProT complex that specifically converts fibrinogen (Fbg) into fibrin...
  15. Hemostatic Factors as Determinants of Bacterial Virulence and Host Defense.
    Jay L Degen; Fiscal Year: 2010
    ..important determinants of the inflammatory response in vivo, ii) bacterial factors known to bind/activate host prothrombin, fibrinogen and plasminogen serve to subvert host defense, iii) leukocyte engagement of fibrin within ..
  16. Studies on anticoagulant properties of thrombin
    Enrico Di Cera; Fiscal Year: 2013
    ..deals with the molecular mechanism of protein C activation by thrombin and its connection with the mechanism of prothrombin activation, as well as with the possibility of turning thrombin into an exclusive activator of protein C for ..
  17. STUDIES OF THROMBIN ALLOSTERY
    Enrico Di Cera; Fiscal Year: 2013
    ..the hypothesis that the E*, E and E:Na+ forms are already present in the most important intermediate along the prothrombin activation pathway...
  18. Mechanisms Linking Hemostatic Facotrs to Neuroinflammatory Disease
    Eric S Mullins; Fiscal Year: 2013
    ..established in the setting of EAE through the use of two complementary approaches: a) diminution of circulating prothrombin by use of an anti- sense oligonucleotide (ASO) gap-mer;and b) the use of a novel mouse line expressing a mutant ..
  19. Thrombin-mediated proteolysis in neuroinflammatory disease
    Jay L Degen; Fiscal Year: 2012
    ..experimental setting, an important experimental asset will be gene-targeted mice with selected alterations in prothrombin or thrombin substrates...
  20. Microactuated Rheometer for Point of Care Coagulopathy Applications
    Richard Superfine; Fiscal Year: 2013
    ..Traditional laboratory coagulation tests such as prothrombin time (PT) and activated partial thromboplastin time (APTT) are both impractical and ineffective at providing ..
  21. INTERACTION OF PLATELETS WITH COAGULATION FACTORS IX & X
    Peter Walsh; Fiscal Year: 1992
    ..weight kininogen, and factor Va and promote the proteolytic activation of factor XII, factor XI, factor X, and prothrombin. To investigate further the activated platelet surface as a locus for the molecular interactions of coagulation ..
  22. RISK FACTORS FOR VENOUS THROMBOEMBOLISM IN THE COMMUNITY
    John A Heit; Fiscal Year: 2013
    ..incidence of VTE;in Aim 2 to determine "when genetic testing is appropriate" by 2a) testing Factor V Leiden, Prothrombin G20210A, and novel ABO SNPs as risk factors for VTE after hospitalization for major surgery and acute medical ..
  23. METAL DEPENDENT COMPLEXES IN BLOOD COAGULATION
    Barbara Furie; Fiscal Year: 2002
    ..This application explores the phospholipid binding specificity of prothrombin and the nature of the prothrombin-membrane binding site...
  24. MOLECULAR INTERACTIONS OF BLOOD COAGULATION FACTOR XA
    Katherine High; Fiscal Year: 1999
    ..The prothrombinase complex catalyzes the conversion of prothrombin to the active serine protease thrombin...
  25. FACTOR VA INTERACTIONS REGULATING PROTHROMBIN ACTIVATION
    Paul E Bock; Fiscal Year: 2010
    ..mechanisms of the procoagulant activation of human factor V, factor Va cofactor activity in regulating prothrombin (ProT) activation, and the mechanism of the anticoagulant inactivation of factor Va by activated protein C (ARC)..
  26. MOLECULAR INTERACTIONS OF BLOOD COAGULATION FACTOR X
    RICHARD PAINTER; Fiscal Year: 1991
    ..associates with factor Va (cofactor) in another membrane surface complex responsible for the activation of prothrombin. The participation of factor X/Xa in these three macromolecular complexes implies that several regions on the ..
  27. Structure/Function Study of the Anticoagulant Protein S
    Alan Rigby; Fiscal Year: 2005
    ..The penultimate step in blood coagulation is the conversion of prothrombin to thrombin, which is then responsible for the conversion of fibrinogen to fibrin...
  28. MUTATIONS, HORMONE THERAPY, AND VENOUS THROMBOEMBOLISM
    Bruce Psaty; Fiscal Year: 2001
    ..between hormone replacement therapy and the prothrombotic mutations, Factor V Leiden and the recently described prothrombin mutation (20210A) on the incidence of VTE in a population-based case-control study conducted at Group Health ..
  29. ANTIBODIES WITH PROTHROMBINASE ACTIVITY IN LUPUS
    Perumal Thiagarajan; Fiscal Year: 2003
    b>Prothrombin is the precursor of thrombin, the central enzyme in blood coagulation...
  30. Biomakers in Sickle Cell Anemia: Response to Hydroxyurea
    Marie Stuart; Fiscal Year: 2004
    ..Markers of coagulation activation will include whole blood tissue factor activity (WBTF) and prothrombin fragment F1.2 levels...
  31. DEFINITION OF FUNCTIONAL SITES ON COAGULATION FACTOR X
    WILLIAM CHURCH; Fiscal Year: 1993
    ..This assembled complex catalyzes the proteolytic activation of prothrombin to the protease thrombin...
  32. VITAMIN K AND SKELETAL HEALTH
    Neil Binkley; Fiscal Year: 2000
    ..vitamin K insufficiency has classically been defined in terms of coagulation abnormalities (prolongation of the prothrombin time), it has recently become appreciated that measurement of undercarboxylation of other proteins, notably ..
  33. PROTHROMBIN ACTIVATION ON BLOOD MONONUCLEAR CELLS
    PAULA TRACY; Fiscal Year: 1990
    ..to assemble an endogenous prothrombinase through synthesis, secretion and activation of factor V, factor X and prothrombin. We will determine how the activated protein C/protein S complex interacts with the monocyte/macrophage surface ..
  34. MEMBRANE BIOPHYSICS AND CONTROL OF THROMBIN GENERATION
    BARRY LENTZ; Fiscal Year: 1999
    ..The working hypothesis is that PS exposure is critical in regulating prothrombin activation because it apparently serves to assemble the prothrombinase, activate factor Xa, place the ..
  35. Factor Va regulation of prothrombinase activity
    JAMILA HIRBAWI; Fiscal Year: 2010
    ..goal of identifying the amino acid residue(s) of factor V that interact with the blood coagulation factor prothrombin (flla), during prothrombinase complex assembly and function...
  36. Development of Platelet & Coagulation Activation Assays
    RONALD SHEBUSKI; Fiscal Year: 2001
    ..markers of platelet activation (membrane and soluble P-selectin and GPIIb/lIIa) and coagulation activation (prothrombin fragment 1...
  37. PROCOAGULANT PROPERTIES OF THE SICKLE CELL MEMBRANE
    Jolyon Jesty; Fiscal Year: 1992
    ..dependence of two major clotting reactions: the activation of factor X by the factor IXa-VIIIa complex, and of prothrombin by the factor Xa-Va complex...
  38. FACTOR VA INTERACTIONS REGULATING PROTHROMBIN ACTIVATION
    Paul Bock; Fiscal Year: 1991
    ..protein-protein interactions underlying the role of factor Va in the mechanism and regulation of human prothrombin activation are proposed...
  39. ACQUISITION OF NMR SPECTROMETER
    William Bachovchin; Fiscal Year: 1991
    ..iii) Vitamin K dependent carboxylation of prothrombin. The 3D solution structures of several prothrombin propeptides are being solved to develop a better understand ..
  40. PATHOPHYSIOLOGY OF ANTIPHOSPHOLIPID ANTIBODY SYNDROMES
    Thomas Ortel; Fiscal Year: 2003
    ..In these patients, autoantibodies that bind to proteins involved in normal hemostasis (e.g., prothrombin) are associated with an increased thrombotic risk...
  41. PROTHROMBINASE COMPLEX--STOPPED-FLOW KINETICS
    Sriram Krishnaswamy; Fiscal Year: 1991
    ..This supramolecular complex catalyses the proteolytic activation of the zymogen prothrombin to the active protease alpha-thrombin...
  42. THROMBOTIC, INFLAMMATORY & GENE MARKERS OF CVD IN WOMEN
    Paul Ridker; Fiscal Year: 2002
    ..plasminogen activator (tPA), plasminogen activator inhibitor type 1 (PAI-1), total plasma homocysteine, prothrombin fragment F1+2, D-dimer, APC-R, C-reactive protein, interleukin-6, and sICAM-1) to determine whether elevations ..
  43. Production of plant-based recombinant human thrombin
    BRIAN HOOKER; Fiscal Year: 2002
    ..The objective of this proposed Phase I SBIR project is to demonstrate expression of correctly processed prothrombin in stable plant cell culture...
  44. VLDL, ATHEROSCLEROSIS, AND THROMBOSIS-MECHANISTIC LINKS
    William Bradley; Fiscal Year: 1993
    ..Basic studies address the hypothesis that HTG-TGRLP are thrombogenic by binding prothrombin, FX(FXa), and providing phospholipid for thrombin generation...
  45. PERINATAL DEVELOPMENT OF VITAMIN K-DEPENDENT HEMOSTASIS
    Edwin Bovill; Fiscal Year: 1993
    ..the presence of significant concentrations of undercarboxylated and thus, dysfunctional protein C and prothrombin in 3-15% of otherwise healthy term and pre-term neonates at birth...
  46. FACTOR II ANTIGEN AND COAGULANT ACTIVITY
    JAMES CORRIGAN; Fiscal Year: 1980
    ..In the proposed research, the conversion of preprothrombin to prothrombin (coagulation factor II) and prothrombin to thrombin will be studied by employing both immunological and ..
  47. Imaging Endocarditis
    Peter Panizzi; Fiscal Year: 2009
    ..S. aureus expresses staphylocoagulase, a bifunctional molecule that activates prothrombin by N-terminal domains and mediates staphylocoagulaseprothrombin complex tethering to the growing vegetations by ..
  48. CA(II)/MG(II) BINDING IN COAGULATION SYSTEMS
    LEE PEDERSEN; Fiscal Year: 1992
    ..molecular description, by experimental and theoretical techniques, of calcium and magnesium ion binding to prothrombin (human and bovine), its Fragment 1 and appropriate fragment peptides in various states of decarboxylation...
  49. MECHANISM OF ACTION OF VITAMIN K AND ANTICOAGULANTS
    Robert Bell; Fiscal Year: 1980
    There is substantial evidence that the vitamin K-epoxide cycle is involved in the synthesis of prothrombin. Agents which block either the regeneration of vitamin K from vitamin K epoxide or the epoxidation of vitamin K are anticoagulants...
  50. THE MECHANISM OF ACTION OF VITAMIN K
    ALLAN WILLINGHAM; Fiscal Year: 1980
    ..We have found that the product of the vitamin K-dependent carboxylation of prothrombin precursor(s) is a protein with the same molecular weight as prothrombin but an isoelectric point of 7.2 (vs. 5...
  51. The Role of Membranes in Thrombus Formation In Viva
    Barbara Furie; Fiscal Year: 2005
    ..of the coagulation enzyme complexes tenase, the activator of factor X and prothrombinase, the activator of prothrombin on the membrane surface...
  52. Thrombin-Stimulated Intrauterine Signaling Pathway
    Mark Phillippe; Fiscal Year: 2007
    ..e. enhanced phospholipid scramblase (PLS), tissue prothrombinase (Fgl2), prothrombin and thrombin receptor (PAR1) expression and activation leading to preterm contractions and delivery...
  53. HOME PROTHROMBIN TIME TESTS
    STEPHEN ZWEIG; Fiscal Year: 1992
    ..Thus, frequent monitoring of blood coagulation rates by prothrombin time tests is required for safe treatment...
  54. THROMBOGENIC PHOSPHOLIPID SURFACES
    FREDERICK DOMBROSE; Fiscal Year: 1980
    ..between the detailed molecular structure of a phospholipid bilayer as a function of: a) its ability to bind prothrombin; and b) enhance the rate of conversion of prothrombin to thrombin by Factor Xa in the presence of an optimal ..
  55. HUMAN RECOMBINANT TISSUE FACTOR PROTHROMBIN TIME REAGENT
    Scott Brown; Fiscal Year: 1991
    DESCRIPTION: (Adapted from the investigator's abstract). The goal of this proposal is to develop a prothrombin time (PT) reagent using purified, reconstituted recombinant human tissue factor (rTF)...
  56. DEVELOPMENT OF A NON-INSTRUMENTED PROTHROMBIN TIME TEST
    WAI LAW; Fiscal Year: 2000
    Development of a disposable non-instrumented prothrombin time test (PF) for home use is proposed. Preliminary data using flow channels built with precision engineered fabric demonstrated the potential of the proposed test device...
  57. Development of a non-instrumental Prothrombin Time test
    WAI LAW; Fiscal Year: 2003
    Development of a disposable non-instrumented prothrombin time (PT) test for home use is proposed. Phase I data using flow channels built with precision woven fabric demonstrated the potential of the proposed test device...
  58. CHARACTERIZATION AND USE OF PROTHROMBIN DEFICIENT MICE
    JAY DEGEN; Fiscal Year: 2005
    b>Prothrombin plays a central role in both the processes of coagulation and anti-coagulation in the blood of mammals, is involved in the stimulation of platelets, is chemotactic for macrophages, and is involved in regulating the ..
  59. MOLECULAR BASIS OF COAGULATION PROTEASE SPECIFICITY
    Alireza R Rezaie; Fiscal Year: 2010
    ..and propagated by the successive activations of the vitamin K-dependent zymogens, factors VII, IX, X and prothrombin;and the non- enzymatic cofactors Va and VIlla that culminate in generation of the fibrinogen clotting enzyme ..
  60. Functional Domains of Coagulation Factor V
    Michael Kalafatis; Fiscal Year: 2007
    ..Both chains of the cofactor interact with factor Xa while only the heavy chain of the cofactor binds prothrombin. The factor Va cofactor activity is efficiently down-regulated following proteolysis of the heavy chain by ..
  61. Novel targets and agents to treat thrombotic disorders
    John Griffin; Fiscal Year: 2004
    ..abstract_text> ..
  62. PROTEIN-MINERAL INTERACTIONS AND CALCIUM OXALATE STONES
    ROSEMARY RYALL; Fiscal Year: 2008
    ..abstract_text> ..
  63. FORMS OF PROSTATE SPECIFIC ANTIGEN AND HK2 IN CANCER
    MARY HEEB; Fiscal Year: 2003
    ..Techniques will include protein purification, antibody and ELISA development, immunoblotting, enzyme activity assays, and peptide synthesis. ..
  64. BLOOD FACTORS AND PERIPHERAL ARTERIAL DISEASE OUTCOMES
    Mary McDermott; Fiscal Year: 2004
    ..The blood factors we will study, fibrinogen, PAI-1, TPA antigen, d-dimer, prothrombin 1...
  65. Reducing Risk Factors in Peripheral Arterial Disease
    Mary Mcgrae McDermott; Fiscal Year: 2010
    ....