cytochromes b5

Summary

Summary: Cytochromes of the b group that are found bound to cytoplasmic side of ENDOPLASMIC RETICULUM. They serve as electron carrier proteins for a variety of membrane-bound OXYGENASES. They are reduced by the enzyme CYTOCHROME-B(5) REDUCTASE.

Top Publications

  1. ncbi The many roles of cytochrome b5
    John B Schenkman
    Department of Pharmacology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA
    Pharmacol Ther 97:139-52. 2003
  2. pmc Apple sucrose transporter SUT1 and sorbitol transporter SOT6 interact with cytochrome b5 to regulate their affinity for substrate sugars
    Ren Chun Fan
    State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing 100094, China
    Plant Physiol 150:1880-901. 2009
  3. ncbi Cloning and transcriptional analysis of Crepis alpina fatty acid desaturases affecting the biosynthesis of crepenynic acid
    Jeong Won Nam
    Department of Chemistry, Washington University in St Louis, One Brookings Drive, Campus Box 1134, St Louis, MO 63130 4899, USA
    J Exp Bot 58:1421-32. 2007
  4. ncbi The cytochromes P450 and b5 and their reductases--promising targets for structural studies by advanced solid-state NMR spectroscopy
    Ulrich H N Dürr
    Biophysics and Department of Chemistry, University of Michigan, 930 N University Avenue, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1768:3235-59. 2007
  5. ncbi A mutation in Arabidopsis cytochrome b5 reductase identified by high-throughput screening differentially affects hydroxylation and desaturation
    Rajesh Kumar
    Institute of Biological Chemistry, Washington State University, Pullman, WA 99164 6340, USA
    Plant J 48:920-32. 2006
  6. ncbi Distinct roles of endoplasmic reticulum cytochrome b5 and fused cytochrome b5-like domain for rat Delta6-desaturase activity
    Herve Guillou
    Laboratoire de Biochimie, Institut National de la Recherche Agronomique Ecole Nationale Supérieure Agronomique, Rennes, France
    J Lipid Res 45:32-40. 2004
  7. ncbi Neurotrophic activity of neudesin, a novel extracellular heme-binding protein, is dependent on the binding of heme to its cytochrome b5-like heme/steroid-binding domain
    Ikuo Kimura
    Laboratory of Applied Pharmacology, Faculty of Pharmacy, Chiba Institute of Science, Choshi, Chiba 288 0025, Japan
    J Biol Chem 283:4323-31. 2008
  8. ncbi The role of cytochrome b5 fusion desaturases in the synthesis of polyunsaturated fatty acids
    Johnathan A Napier
    IACR Long Ashton Research Station, Long Ashton, Bristol BS41 9AF, UK
    Prostaglandins Leukot Essent Fatty Acids 68:135-43. 2003
  9. ncbi Sheep adrenal cytochrome b5: active as a monomer or a tetramer in vivo?
    Nicolaas Lombard
    Department of Biochemistry, University of Stellenbosch, Matieland 7602, South Africa
    Endocr Res 28:485-92. 2002
  10. ncbi Tobacco cytochrome b5: cDNA isolation, expression analysis and in vitro protein targeting
    M A Smith
    Department of Botany, University of Bristol, UK
    Plant Mol Biol 25:527-37. 1994

Research Grants

  1. ROLE OF HYDROXYLAMINE REDUCTION IN DRUG HYPERSENSITIVITY
    Lauren Trepanier; Fiscal Year: 2004
  2. ENZYMES AND REACTIONS FOR REPAIR OF DNA IN HUMAN CELLS
    THOMAS BRENT; Fiscal Year: 1993
  3. Novel Mechanisms of Quinone Toxicity
    David Ross; Fiscal Year: 2010
  4. Prostaglandin 19-& 20-Hydroxylation by Cytochrome P450
    Bettie Sue Masters; Fiscal Year: 2005
  5. Nitogen reduction and xenobiotic response
    Lauren A Trepanier; Fiscal Year: 2010
  6. Nitogen reduction and xenobiotic response
    Lauren Trepanier; Fiscal Year: 2007
  7. Nitogen reduction and xenobiotic response
    Lauren Trepanier; Fiscal Year: 2009
  8. ELECTRON TRANSFER PROCESSES IN IRON AND COPPER PROTEINS
    Harry Gray; Fiscal Year: 1990
  9. CYTOCHROMES B: MODELS & PROTEIN STUDIES
    Frances Walker; Fiscal Year: 1991
  10. PARTICIPATION OF CYTOCHROME B5 IN ANESTHETIC METABOLISM
    Lucy Waskell; Fiscal Year: 2002

Detail Information

Publications198 found, 100 shown here

  1. ncbi The many roles of cytochrome b5
    John B Schenkman
    Department of Pharmacology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA
    Pharmacol Ther 97:139-52. 2003
    ..This effector function may be to enhance the breakdown of the oxygenated hemoprotein to products or to facilitate flow of electrons through the system...
  2. pmc Apple sucrose transporter SUT1 and sorbitol transporter SOT6 interact with cytochrome b5 to regulate their affinity for substrate sugars
    Ren Chun Fan
    State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing 100094, China
    Plant Physiol 150:1880-901. 2009
    ..These findings suggest a novel mechanism by which the plant cells tailor sugar uptake to the surrounding sugar availability...
  3. ncbi Cloning and transcriptional analysis of Crepis alpina fatty acid desaturases affecting the biosynthesis of crepenynic acid
    Jeong Won Nam
    Department of Chemistry, Washington University in St Louis, One Brookings Drive, Campus Box 1134, St Louis, MO 63130 4899, USA
    J Exp Bot 58:1421-32. 2007
    ..Acetylenase transcription is observed in both species without accumulation of the enzymatic product, crepenynate. Functional expression of acetylenase appears to be affected by competition and collaboration with other enzymes...
  4. ncbi The cytochromes P450 and b5 and their reductases--promising targets for structural studies by advanced solid-state NMR spectroscopy
    Ulrich H N Dürr
    Biophysics and Department of Chemistry, University of Michigan, 930 N University Avenue, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1768:3235-59. 2007
    ..We aim to give a detailed account of modern techniques as applicable to cyt P450 and cyt b5, to show what has already been possible and what seems to be viable in the very near future...
  5. ncbi A mutation in Arabidopsis cytochrome b5 reductase identified by high-throughput screening differentially affects hydroxylation and desaturation
    Rajesh Kumar
    Institute of Biological Chemistry, Washington State University, Pullman, WA 99164 6340, USA
    Plant J 48:920-32. 2006
    ..These results suggest that there is competition in developing seeds among the several reactions that utilize reduced cytochrome b5...
  6. ncbi Distinct roles of endoplasmic reticulum cytochrome b5 and fused cytochrome b5-like domain for rat Delta6-desaturase activity
    Herve Guillou
    Laboratoire de Biochimie, Institut National de la Recherche Agronomique Ecole Nationale Supérieure Agronomique, Rennes, France
    J Lipid Res 45:32-40. 2004
    ..These data suggest distinct and essential roles for both the desaturase cytochrome b5-like domain and free endoplasmic reticulum cytochrome b5 for Delta6-desaturase activity...
  7. ncbi Neurotrophic activity of neudesin, a novel extracellular heme-binding protein, is dependent on the binding of heme to its cytochrome b5-like heme/steroid-binding domain
    Ikuo Kimura
    Laboratory of Applied Pharmacology, Faculty of Pharmacy, Chiba Institute of Science, Choshi, Chiba 288 0025, Japan
    J Biol Chem 283:4323-31. 2008
    ..Neudesin is the first extracellular heme-binding protein that shows signal transducing activity by itself. The present findings may shed new light on the function of extracellular heme-binding proteins...
  8. ncbi The role of cytochrome b5 fusion desaturases in the synthesis of polyunsaturated fatty acids
    Johnathan A Napier
    IACR Long Ashton Research Station, Long Ashton, Bristol BS41 9AF, UK
    Prostaglandins Leukot Essent Fatty Acids 68:135-43. 2003
    ..More importantly, this knowledge helps underpin our understanding of polyunsaturated fatty acid biosynthesis...
  9. ncbi Sheep adrenal cytochrome b5: active as a monomer or a tetramer in vivo?
    Nicolaas Lombard
    Department of Biochemistry, University of Stellenbosch, Matieland 7602, South Africa
    Endocr Res 28:485-92. 2002
    ..Immunoblot analyses and immunocytochemistry of adrenal tissue with antibodies against the full length cyt b5 indicated that the tetrameric form of the protein was in all probability the dominant specie in vivo...
  10. ncbi Tobacco cytochrome b5: cDNA isolation, expression analysis and in vitro protein targeting
    M A Smith
    Department of Botany, University of Bristol, UK
    Plant Mol Biol 25:527-37. 1994
    ..In vitro transcription and translation studies of the cDNA indicated that the protein inserts into the ER membrane by a non-SRP-mediated pathway and that the C-terminus of the protein is required for targeting and insertion...
  11. ncbi Two homologous cytochromes b5 in a single cell
    F Lederer
    Eur J Biochem 132:95-102. 1983
    The amino acid sequence of the heme-binding domains of rat liver cytochromes b5 from outer mitochondrial membranes and from microsomes has been determined by a combination of automatic and manual degradation of fragments generated by ..
  12. ncbi Human cytochrome b5 requires residues E48 and E49 to stimulate the 17,20-lyase activity of cytochrome P450c17
    Jacqueline L Naffin-Olivos
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 8857, USA
    Biochemistry 45:755-62. 2006
    ..These data suggest that a specific allosteric binding site on b5, which includes residues E48, E49, and possibly R52, mediates the stimulation of 17,20-lyase activity...
  13. ncbi The distinct heme coordination environments and heme-binding stabilities of His39Ser and His39Cys mutants of cytochrome b5
    Wen Hu Wang
    Chemical Biology Laboratory, Department of Chemistry and State Key Laboratory of Genetics, School of Life Science, Fudan University, Shanghai 200433, P R China
    Protein Eng 16:1047-54. 2003
    ..These two mutants were also found to have quite lower heme-binding stabilities. The order of stabilities of ferric proteins is: wild-type cytochrome b(5) >> His39Cys > His39Ser...
  14. ncbi Design challenges for hemoproteins: the solution structure of apocytochrome b5
    C J Falzone
    Department of Chemistry, Pennsylvania State University, University Park 16802, USA
    Biochemistry 35:6519-26. 1996
    ....
  15. ncbi A relationship between heme binding and protein stability in cytochrome b5
    Kunal Mukhopadhyay
    Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 43:12227-36. 2004
    ..These data supported that heme affinity could be modulated by the apoprotein stability and by specific residues remote from the heme binding site...
  16. doi Isolated 17,20-lyase deficiency due to the cytochrome b5 mutation W27X
    Renée C Kok
    Endocrine Laboratory, Department of Internal Medicine, Erasmus Medical Centre, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    J Clin Endocrinol Metab 95:994-9. 2010
    ..Conclusion: We demonstrated 17,20-lyase deficiency due to an aberrant CytB5. Our findings thus provide evidence for an alternative etiology for this disorder...
  17. ncbi The role of cytochrome b5 in 4alpha-methyl-oxidation and C5(6) desaturation of plant sterol precursors
    A Rahier
    Département d Enzymologie Cellulaire et Moléculaire, Institut de Biologie Moleculaire des Plantes, CNRS UPR 406, Strasbourg, France
    Biochem Biophys Res Commun 236:434-7. 1997
    ..These results strongly suggest that membrane-bound cytochrome b5 of maize microsomes is an obligatory electron carrier from NAD(P)H to 4,4-DMSO, 4alpha-MSO, and 5-DES...
  18. pmc Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6-desaturated fatty acids in transgenic tobacco
    O Sayanova
    Institute of Arable Crops Research Long Ashton Research Station, University of Bristol, United Kingdom
    Proc Natl Acad Sci U S A 94:4211-6. 1997
    ..6% of the total fatty acids, respectively. The borage delta6-fatty acid desaturase differs from other desaturase enzymes, characterized from higher plants previously, by the presence of an N-terminal domain related to cytochrome b5...
  19. pmc Distinct targeting pathways for the membrane insertion of tail-anchored (TA) proteins
    Vincenzo Favaloro
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, DKFZ ZMBH Allianz, Heidelberg, Germany
    J Cell Sci 121:1832-40. 2008
    ..The Asna1-mediated pathway of membrane insertion of RAMP4 and Sec61beta may relate to functions of these proteins in the ER stress response...
  20. ncbi The kinetic and spectral characterization of the E. coli-expressed mammalian CYP4A7: cytochrome b5 effects vary with substrate
    P A Loughran
    Department of Biochemistry, The University of Texas Health Science Center, San Antonio 78229-3900, USA
    Arch Biochem Biophys 385:311-21. 2001
    ..Estimations of the rate of CPR (0.3 s(-1)) or cytochrome b5 (9.1 s(-1)) binding with CYP4A7 were also determined...
  21. ncbi Congenital methemoglobinemia with a deficiency of cytochrome b5
    E Hegesh
    N Engl J Med 314:757-61. 1986
  22. ncbi Comparison of cytochromes b5 from insects and vertebrates
    Lijun Wang
    Department of Chemistry, University of Kansas, Lawrence, Kansas 66045, USA
    Proteins 67:293-304. 2007
    ....
  23. ncbi A novel cytochrome b5-like domain is linked to the carboxyl terminus of the Saccharomyces cerevisiae delta-9 fatty acid desaturase
    A G Mitchell
    Department of Biological Sciences, Rutgers University, Nelson Laboratories, Piscataway, New Jersey 08855 1059, USA
    J Biol Chem 270:29766-72. 1995
    ....
  24. ncbi P450c17 mutations R347H and R358Q selectively disrupt 17,20-lyase activity by disrupting interactions with P450 oxidoreductase and cytochrome b5
    D H Geller
    Department of Pediatrics, University of California, San Francisco 94143 0978, USA
    Mol Endocrinol 13:167-75. 1999
    ..Furthermore, the data offer the first evidence that R347 is a crucial component of the site at which b5 interacts with the P450c17 x OR complex to promote electron transfer...
  25. ncbi Roles of NADPH-P450 reductase and apo- and holo-cytochrome b5 on xenobiotic oxidations catalyzed by 12 recombinant human cytochrome P450s expressed in membranes of Escherichia coli
    Hiroshi Yamazaki
    Faculty of Pharmaceutical Sciences, Kanazawa University, 13 1 Takara machi, Kanazawa 920 0934, Japan
    Protein Expr Purif 24:329-37. 2002
    ..P450/NPR membrane systems containing b5 are useful models for prediction of the rates for liver microsomal P450-dependent drug oxidations...
  26. doi Asna1/TRC40-mediated membrane insertion of tail-anchored proteins
    Vincenzo Favaloro
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Allianz, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    J Cell Sci 123:1522-30. 2010
    ..By contrast, membrane insertion of cytochrome b5 can proceed independently of Asna1 and nucleotides...
  27. doi Cytochrome b5, not superoxide anion radical, is a major reductant of indoleamine 2,3-dioxygenase in human cells
    Ghassan J Maghzal
    Centre for Vascular Research and Molecular Immunopathology Unit, Bosch Institute and Discipline of Pathology, School of Medical Sciences, University of Sydney, Sydney, New South Wales 2006, Australia
    J Biol Chem 283:12014-25. 2008
    ..Together, our data show that cytochrome b(5) rather than O(2)(*-) plays a major role in the activation of IDO in human cells...
  28. pmc Two tail-anchored protein variants, differing in transmembrane domain length and intracellular sorting, interact differently with lipids
    Paolo Ceppi
    Department of Experimental, Environmental Medicine, and Biotechnologies DIMESAB, Medical School, University of Milano Bicocca, 20052 Monza, Italy
    Proc Natl Acad Sci U S A 102:16269-74. 2005
    ..The dissimilar interaction with lipids of these two differently localized TA proteins could have implications for their intracellular sorting...
  29. pmc Defining the in Vivo Role for cytochrome b5 in cytochrome P450 function through the conditional hepatic deletion of microsomal cytochrome b5
    Robert D Finn
    Cancer Research UK Molecular Pharmacology Unit, Biomedical Research Institute, Level 5, Ninewells Hospital and Medical School, Dundee DD1 9SY, United Kingdom
    J Biol Chem 283:31385-93. 2008
    ..6-fold in hepatic microsomal cytochrome b5 null mice. These data indicate that microsomal cytochrome b5 can play a major role in the in vivo metabolism of certain drugs and chemicals but in a P450- and substrate-dependent manner...
  30. pmc Reduction of hexavalent chromium by human cytochrome b5: generation of hydroxyl radical and superoxide
    Griselda R Borthiry
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Free Radic Biol Med 42:738-55; discussion 735-7. 2007
    ..Overall, the simultaneous generation of Cr(V) and H(2)O(2) by the PLs and the resulting generation of HO() at low Cr(VI) concentrations could have important implications for Cr(VI) toxicity...
  31. pmc Reductive detoxification of arylhydroxylamine carcinogens by human NADH cytochrome b5 reductase and cytochrome b5
    Joseph R Kurian
    Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, Wisconsin 53706 1102, USA
    Chem Res Toxicol 19:1366-73. 2006
    ....
  32. pmc NCB5OR is a novel soluble NAD(P)H reductase localized in the endoplasmic reticulum
    Hao Zhu
    Hematology Division, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:30316-25. 2004
    ..The subcellular localization and redox properties of NCB5OR provide important insights into the biology of NCB5OR and the phenotype of the Ncb5or-null mouse...
  33. pmc Novel targeting signals mediate the sorting of different isoforms of the tail-anchored membrane protein cytochrome b5 to either endoplasmic reticulum or mitochondria
    Yeen Ting Hwang
    Department of Botany, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    Plant Cell 16:3002-19. 2004
    ..Collectively, the results presented provide significant insight into the early biogenetic events required for entry of tail-anchored proteins into either the ER or mitochondrial targeting pathways...
  34. ncbi Intracellular sorting of the tail-anchored protein cytochrome b5 in plants: a comparative study using different isoforms from rabbit and Arabidopsis
    Caterina Maggio
    CNR Istituto di Biologia e Biotecnologia Agraria, via Bassini 15, Milano, Italy
    J Exp Bot 58:1365-79. 2007
    ....
  35. ncbi Approach to predict the contribution of cytochrome P450 enzymes to drug metabolism in the early drug-discovery stage: the effect of the expression of cytochrome b(5) with recombinant P450 enzymes
    C Emoto
    Department of Pharmacokinetics Dynamics Metabolism, Pfizer Global Research and Development, Nagoya Laboratories, Pfizer Japan Inc, Taketoyo, Aichi, Japan
    Xenobiotica 37:986-99. 2007
    ..In addition, recombinant CYP microsomes co-expressed with cytochrome b(5) might be suitable for the prediction of the CL(int) value...
  36. pmc Targeting of a tail-anchored protein to endoplasmic reticulum and mitochondrial outer membrane by independent but competing pathways
    N Borgese
    Consiglio Nazionale delle Ricerche, Cellular and Molecular Pharmacology Center and Department of Medical Pharmacology, University of Milan, Milan, Italy
    Mol Biol Cell 12:2482-96. 2001
    ..Thus, targeting occurs directly from the cytosol. Moreover, ER and MOM compete for the same polypeptide, explaining the dual localization of some TA proteins...
  37. ncbi Cytochrome b5 inhibits electron transfer from NADPH-cytochrome P450 reductase to ferric cytochrome P450 2B4
    Haoming Zhang
    Department of Anesthesiology, University of Michigan Medical School and Veterans Affairs Medical Center, Ann Arbor, Michigan 48105, USA
    J Biol Chem 283:5217-25. 2008
    ....
  38. ncbi Probing the differences between rat liver outer mitochondrial membrane cytochrome b5 and microsomal cytochromes b5
    A Altuve
    Department of Chemistry, Oklahoma State University, Stillwater 74078 3071, USA
    Biochemistry 40:9469-83. 2001
    ..negative reduction potential and is substantially more stable toward chemical and thermal denaturation than Mc cytochromes b5. In addition, hemin is kinetically trapped in rat OM cyt b5 but not in the Mc proteins...
  39. ncbi The roles of cytochrome b5 in cytochrome P450 reactions
    Todd D Porter
    Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY 40536 0082, USA
    J Biochem Mol Toxicol 16:311-6. 2002
    ..Evidence now indicates that each of these pathways is likely to operate in vivo...
  40. ncbi X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35-->Tyr mutant
    Ping Yao
    Chemical Biology Laboratory, Department of Chemistry, Fudan University, Shanghai, People s Republic of China
    Eur J Biochem 269:4287-96. 2002
    ..The detailed conformational changes of the proteins in response to heat have been studied by CD for the first time, revealing the existence of the folding intermediate...
  41. ncbi Trafficking of tail-anchored proteins: transport from the endoplasmic reticulum to the plasma membrane and sorting between surface domains in polarised epithelial cells
    Alessandra Bulbarelli
    Consiglio Nazionale delle Ricerche Cellular and Molecular Pharmacology Center and Department of Medical Pharmacology, University of Milan, Milan, Italy
    J Cell Sci 115:1689-702. 2002
    ..The two GFP fusion proteins showed a different surface distribution, which is consistent with a role for the two syntaxin TMDs in polarised sorting...
  42. doi The role of cytosolic proteins in the insertion of tail-anchored proteins into phospholipid bilayers
    Sara F Colombo
    CNR Institute for Neuroscience and Department of Pharmacology, Universita degli Studi di Milano, Italy
    J Cell Sci 122:2383-92. 2009
    ..The effect of diamide is also observed in living cells. Thus, the specific in vivo targeting of b5 might be achieved by interaction with redox-sensitive targeting factors that hinder its nonspecific insertion into any permissive bilayer...
  43. ncbi Assessment of the ability of type 2 cytochrome b5 to modulate 17,20-lyase activity of human P450c17
    Penny Soucy
    Oncology and Molecular Endocrinology Research Center, Laval University Medical Center CHUL and Laval University, Ste Foy Que, Canada G1V 4G2
    J Steroid Biochem Mol Biol 80:71-5. 2002
    ..Furthermore, the analysis of mRNA expression of types 1 and 2 cyt-b5 by RT-PCR using primers specific to each type showed that both types are present in the liver but also in the adrenal and testis...
  44. pmc Transmembrane topogenesis of a tail-anchored protein is modulated by membrane lipid composition
    Silvia Brambillasca
    CNR Institute of Neuroscience Cell Mol Pharmacology and Department of Medical Pharmacology, University of Milan, Milan, Italy
    EMBO J 24:2533-42. 2005
    ..These results identify the minimal requirements for transmembrane topogenesis of a TA protein and suggest that selectivity among various intracellular compartments can be imparted by differences in their lipid composition...
  45. pmc Membrane-bound progesterone receptors contain a cytochrome b5-like ligand-binding domain
    William Mifsud
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Genome Biol 3:RESEARCH0068. 2002
    ..They do not show sequence similarity to any of the classical steroid receptors. We were interested in identifying distant homologs of MAPR better to understand their biological roles...
  46. ncbi Cytochrome b(5) modulation of 17{alpha} hydroxylase and 17-20 lyase (CYP17) activities in steroidogenesis
    M K Akhtar
    Institute of Biological Sciences, Cledwyn Building, University of Wales, Aberystwyth, Ceredigion SY23 3DD, UK
    J Endocrinol 187:267-74. 2005
    ....
  47. ncbi Mammalian mitochondrial and microsomal cytochromes b(5) exhibit divergent structural and biophysical characteristics
    Adriana Altuve
    Department of Chemistry, University of Kansas, Lawrence, KS 66047, USA
    Biochem Biophys Res Commun 314:602-9. 2004
    ..The biophysical properties characteristic of the OM proteins may be important in facilitating the two functions proposed for them so far, reduction of ascorbate radical and stimulation of androgen synthesis...
  48. ncbi 1H NMR studies of the effect of mutation at Valine45 on heme microenvironment of cytochrome b5
    Chunyang Cao
    State Key Laboratory of Bio organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 354 Feng Lin Lu Road, Shanghai 200032, China
    Biochimie 85:1007-16. 2003
    ..The relationships between the structure and biological function of the mutants are discussed in terms of the geometry of heme and axial ligands, the hydrophobicity of heme pocket and the electrostatic potential of the heme-exposed area...
  49. ncbi Determination of the rate of reduction of oxyferrous cytochrome P450 2B4 by 5-deazariboflavin adenine dinucleotide T491V cytochrome P450 reductase
    Haoming Zhang
    University of Michigan and VA Medical Research Center, 2215 Fuller Road, Ann Arbor, Michigan 48105, USA
    Biochemistry 42:11594-603. 2003
    ....
  50. ncbi The solution structure of the oxidized bovine microsomal cytochrome b(5) mutant V61H
    Chunyang Cao
    State Key Laboratory of Bio organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, PR China
    Biochem Biophys Res Commun 307:600-9. 2003
    ..Additionally, the axial ligand rings counterclockwise rotate around His39 N-Fe axis due to the mutation, which is confirmed by variation of the hyperfine shifts of the heme protons of V61H compared to those of WT cytochrome b(5)...
  51. ncbi Cytochrome b(5) coexpression increases the CYP2E1-dependent mutagenicity of dialkylnitrosamines in methyltransferase-deficient strains of Salmonella typhimurium
    M T Cooper
    Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536-0305, USA
    Mutat Res 484:61-8. 2001
    ..These new, highly sensitive strains may prove useful in the detection of nitrosamine contamination of food and environmental samples...
  52. ncbi Cytochrome b5 reductase and cytochrome b5 support the CYP2E1-mediated activation of nitrosamines in a recombinant Ames test
    Vishwesh Mokashi
    Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536 0305, USA
    Arch Biochem Biophys 412:147-52. 2003
    ..Neither cytochrome b(5) reductase nor cytochrome b(5) alone could support CYP2E1 activity. These results demonstrate that the cytochrome b(5) reductase/cytochrome b(5) pathway can support CYP2E1 activity in bacterial cells...
  53. ncbi Hepatic cytochrome P450 reductase-null mice reveal a second microsomal reductase for squalene monooxygenase
    Li Li
    Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY 40536, USA
    Arch Biochem Biophys 461:76-84. 2007
    ....
  54. ncbi Cytochrome b5 increases the rate of product formation by cytochrome P450 2B4 and competes with cytochrome P450 reductase for a binding site on cytochrome P450 2B4
    Haoming Zhang
    Department of Anesthesiology, University of Michigan Medical School, Ann Arbor, Michigan 48105, USA
    J Biol Chem 282:29766-76. 2007
    ..It is proposed that cyt b(5) and CPR compete for a binding site on cyt P450 2B4...
  55. doi A web-based resource for the Arabidopsis P450, cytochromes b5, NADPH-cytochrome P450 reductases, and family 1 glycosyltransferases (http://www.P450.kvl.dk)
    Suzanne M Paquette
    Department of Biological Structure, University of Washington, HSB G 520 Box 357420, Seattle, WA 98195 7420, USA
    Phytochemistry 70:1940-7. 2009
    ..P450.kvl.dk) is a sequence repository of manually curated sequences, multiple sequence alignments, phylogenetic trees, sequence motif logos, 3D structures, intron-exon maps, and customized BLAST datasets...
  56. ncbi Lack of electron transfer from cytochrome b5 in stimulation of catalytic activities of cytochrome P450 3A4. Characterization of a reconstituted cytochrome P450 3A4/NADPH-cytochrome P450 reductase system and studies with apo-cytochrome b5
    H Yamazaki
    Osaka Prefectural Institute of Public Health, Osaka 537, Japan
    J Biol Chem 271:27438-44. 1996
    ..We conclude that b5 can facilitate some P450 3A4-catalyzed oxidations by complexing with P450 3A4 and enhancing its reduction by NADPH-P450 reductase, without directly transferring electrons to P450...
  57. ncbi Molecular cloning, overexpression in Escherichia coli, structural and functional characterization of house fly cytochrome b5
    V M Guzov
    Department of Entomology, University of Arizona, Tucson, Arizona 85721, USA
    J Biol Chem 271:26637-45. 1996
    ..Absorption and EPR spectroscopy reveal properties very similar to cytochromes b5 from vertebrates...
  58. ncbi The reduction potential of cytochrome b5 is modulated by its exposed heme edge
    M Rivera
    Department of Chemistry, Oklahoma State University, Stillwater 74078 3071, USA
    Biochemistry 37:1485-94. 1998
    ..The negative shift in the reduction potential of the mutant protein was explained by the increased accessibility of water to the heme binding site, as observed in its X-ray crystal structure...
  59. ncbi Solution structure of reduced microsomal rat cytochrome b5
    L Banci
    Department of Chemistry, University of Florence, Italy
    Eur J Biochem 249:270-9. 1997
    ..The structural features in solution of the residues proposed to be involved in protein-protein recognition are found to be largely conserved with respect to the solid state...
  60. ncbi Characterization of heme-coordinating histidyl residues of cytochrome b5 based on the reactivity with diethylpyrocarbonate: a mechanism for the opening of axial imidazole rings
    Nobuyuki Nakanishi
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, Rokkodai cho, Nada Ku, Kobe, Hyogo 657 8501
    J Biochem 140:561-71. 2006
    ....
  61. ncbi Trypanosoma brucei oleate desaturase may use a cytochrome b5-like domain in another desaturase as an electron donor
    Guillermo A Petrini
    Instituto de Biologia Molecular y Celular de Rosario IBR, CONICET, Departamento de Microbiologia, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Santa Fe, Argentina
    Eur J Biochem 271:1079-86. 2004
    ..Thus, Ole1p has, like Cytb5p, an additional cytochrome b5 function (actually an electron donor function), which is responsible for the activity detected when using the cytb5Delta single mutant...
  62. pmc Cytochrome b5 null mouse: a new model for studying inherited skin disorders and the role of unsaturated fatty acids in normal homeostasis
    Robert D Finn
    School of Applied Sciences, Ellison Building, Northumbria University, Newcastle, NE1 8ST, UK
    Transgenic Res 20:491-502. 2011
    ..These data demonstrate that regional concentrations of unsaturated fatty acids are controlled by endogenous metabolic pathways and not by diet alone...
  63. ncbi Structure and characterization of Ectothiorhodospira vacuolata cytochrome b(558), a prokaryotic homologue of cytochrome b(5)
    V Kostanjevecki
    Department of Biochemistry, Laboratory of Protein Biochemistry and Protein Engineering, University of Gent, B 9000 Gent, Belgium
    J Biol Chem 274:35614-20. 1999
    ..A search of the Mycobacterium tuberculosis genome showed that a previously unidentified gene encodes a fatty-acid desaturase with an N-terminal b(5) domain. Thus, it may provide another example of a bacterial b(5) homologue...
  64. ncbi An adult-specific CYP6 P450 gene is overexpressed in a pyrethroid-resistant strain of the malaria vector, Anopheles gambiae
    Dimitra Nikou
    Vector Biology Division, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, UK
    Gene 318:91-102. 2003
    ..The isolation of An. gambiae cDNAs encoding cytochrome b(5) and nicotinamide adenine dinucleotide phosphate (reduced form) (NADPH)-cytochrome P450 reductase cDNAs is also reported...
  65. ncbi Insulin in flavin-containing monooxygenase regulation. Flavin-containing monooxygenase and cytochrome P450 activities in experimental diabetes
    Tímea Borbás
    Division of Pharmacology and Drug Safety, Richter Gedeon Ltd, Gyomroi ut 19 21, 1475 Budapest, P O Box 27, Hungary
    Eur J Pharm Sci 28:51-8. 2006
    ..Blood glucose level is a good marker for FMO induction. The marked reduction of intestinal CYP3A capacity suggests that diabetes exerts a substantial effect on the activity of most determining intestinal CYP enzyme...
  66. ncbi Examining the mechanism of stimulation of cytochrome P450 by cytochrome b5: the effect of cytochrome b5 on the interaction between cytochrome P450 2B4 and P450 reductase
    James R Reed
    Department of Drug Metabolism, Merck and Co, PO Box 2000, Rahway, NJ 07065, USA
    J Inorg Biochem 97:265-75. 2003
    ..We propose that the conformational change(s) necessary for progression of the catalytic cycle is inhibited when reduced, but not oxidized, reductase is bound to the P450...
  67. ncbi Subcellular/tissue distribution and responses to oil exposure of the cytochrome P450-dependent monooxygenase system and glutathione S-transferase in freshwater prawns (Macrobrachium malcolmsonii, M. lamarrei lamarrei)
    S Arun
    Central Electrochemical Research Institute, Karaikudi 630 006, Tamil Nadu, India
    Ecotoxicology 15:341-6. 2006
    ....
  68. ncbi Therapeutic effect of propolis and paclitaxel on hepatic phase I and II enzymes and marker enzymes in dimethylbenz(a)anthracene-induced breast cancer in female rats
    Radhakrishnan Padmavathi
    Department of Medical Biochemistry, University of Madras, Taramani Campus, Chennai 600 113, Tamilnadu, India
    Comp Biochem Physiol C Toxicol Pharmacol 143:349-54. 2006
    ..On the basis of the observed results propolis can be considered a promising chemotherapeutic agent and can be administered as an adjuvant with paclitaxel chemotherapy...
  69. ncbi Molecular dynamics simulation studies of cytochrome b5 from outer mitochondrial and microsomal membrane
    Kyung Hoon Lee
    Department of Chemistry, University of Kansas, 1251 Wescoe Hall Drive, Room 2010, Malott Hall, Lawrence, KS 66045 7582, USA
    Biopolymers 69:260-9. 2003
    ..The OM protein also appears to have a more compact hydrophobic core in its beta-sheet region. These effects may be used to explain observed stability differences between the two proteins...
  70. ncbi Biochemical assessment of limits to estrogen synthesis in porcine follicles
    C J Corbin
    Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, California 95616, USA
    Biol Reprod 69:390-7. 2003
    ..Neither reductase nor cytochrome b5 appear to regulate P450 activities, but the expression of cytochrome b5 in granulosa and theca suggests possible alternative roles for this protein in follicular development or function...
  71. ncbi Characterization of the interaction of amphipathic cytochrome b5 with stearyl coenzyme A desaturase and NADPH:cytochrome P-450 reductase
    H A Dailey
    J Biol Chem 255:5184-9. 1980
  72. ncbi Zinc mediates normalization of hepatic drug metabolizing enzymes in chlorpyrifos-induced toxicity
    Ajay Goel
    Department of Biophysics, Panjab University, Sector 14, Chandigarh 160014, India
    Toxicol Lett 169:26-33. 2007
    ....
  73. ncbi Effects of chronic exposure to cadmium on renal cytochrome P450-dependent monooxygenase system in rats
    Andrzej Plewka
    Department of Histology and Embryology, Medical University of Silesia, Medyków 20, 40 752 Katowice Ligota, Poland
    Arch Toxicol 78:194-200. 2004
    ..Thus, we hypothesize that the metabolism and detoxification of many substances, including xenobiotics, may be seriously affected in Cd-exposed subjects...
  74. ncbi Enhancing the stability of microsomal cytochrome b5: a rational approach informed by comparative studies with the outer mitochondrial membrane isoform
    Na Sun
    Department of Chemistry, University of Kansas, 1251 Wescoe Hall Drive, Lawrence, KS 66045 7582, USA
    Protein Eng Des Sel 18:571-9. 2005
    ..The results suggest that maximizing heme binding pocket compactness in the apo state is a useful general strategy for increasing the stability of engineered or designed proteins...
  75. ncbi Effects of cytochrome b(5) on drug oxidation activities of human cytochrome P450 (CYP) 3As: similarity of CYP3A5 with CYP3A4 but not CYP3A7
    Satoshi Yamaori
    Laboratory of Drug Metabolism, Graduate School of Pharmaceutical Sciences, Hokkaido University, 060 0812 Sapporo, Japan
    Biochem Pharmacol 66:2333-40. 2003
    ..The effects of b(5) on kinetic parameters of CYP3A5 were similar to those of CYP3A4 but not CYP3A7. These results suggest that roles of b(5) in drug oxidation activities of CYP3A5 and CYP3A4 are different from those of CYP3A7...
  76. ncbi Interactions of mammalian cytochrome P450, NADPH-cytochrome P450 reductase, and cytochrome b(5) enzymes
    Tsutomu Shimada
    Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, 638 Robinson Research Building, 23rd and Pierce Avenues, Nashville, TN 37232 0146, USA
    Arch Biochem Biophys 435:207-16. 2005
    ....
  77. ncbi Redundancy in the pathway for redox regulation of mammalian methionine synthase: reductive activation by the dual flavoprotein, novel reductase 1
    Horatiu Olteanu
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 278:38310-4. 2003
    ....
  78. ncbi Short-term inhibitory effects of nitric oxide on cytochrome P450-mediated drug metabolism: time dependency and reversibility profiles in isolated perfused rat livers
    Ragini Vuppugalla
    School of Pharmacy, Texas Tech University Health Sciences Center, 1300 S Coulter, Amarillo, TX 79106, USA
    Drug Metab Dispos 32:1446-54. 2004
    ..The short-term effects of NO on P450 are time-dependent and enzyme-selective, with both reversible and irreversible mechanisms...
  79. ncbi Inhibition of CYP2E1 catalytic activity in vitro by S-adenosyl-L-methionine
    Andres A Caro
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY 10029, USA
    Biochem Pharmacol 69:1081-93. 2005
    ....
  80. pmc Stabilizing roles of residual structure in the empty heme binding pockets and unfolded states of microsomal and mitochondrial apocytochrome b5
    Aaron B Cowley
    Department of Chemistry, University of Kansas, 1251 Wescoe Hall Drive, 2010 Malott Hall, Lawrence, KS 66045 7582, USA
    Protein Sci 13:2316-29. 2004
    ..We propose that a more robust beta-sheet in Mc apo-b5s compensates for the absence of the hydrophobic packing interactions that stabilize the heme binding pocket in OM apo-b5s...
  81. ncbi Role of cytochrome B5 in modulating peroxide-supported cyp3a4 activity: evidence for a conformational transition and cytochrome P450 heterogeneity
    Santosh Kumar
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1031, USA
    Drug Metab Dispos 33:1131-6. 2005
    ..In conclusion, the interaction of b(5) with P450 apparently leads to a conformational transition, which results in redistribution of the CYP3A4 pool...
  82. ncbi The role of the length and sequence of the linker domain of cytochrome b5 in stimulating cytochrome P450 2B4 catalysis
    Thomas A Clarke
    Centre of Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Biol Chem 279:36809-18. 2004
    ..A model for the membrane-bound cyt b(5)-cyt P450 complex is presented...
  83. ncbi Reduction of cytochrome b5 by NADPH-cytochrome P450 reductase
    F Peter Guengerich
    Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Arch Biochem Biophys 440:204-11. 2005
    ..07 s(-1)), very fast reduction, and dissociation. In vesicles, encounter is rapid and the slow step (approximately 3 s(-1)) is reduction within a complex less favorable for reduction than in the non-vesicle systems...
  84. ncbi Induction of rat hepatic cytochrome P-450 by ketamine and its toxicological implications
    Wei Hung Chan
    Institute of Toxicology, National Taiwan University, Taipei, Taiwan
    J Toxicol Environ Health A 68:1581-97. 2005
    ..Other potential pharmacological or toxicological events related to ketamine use need to be further explored...
  85. ncbi Neuronal calcium sensor protein visinin-like protein-3 interacts with microsomal cytochrome b5 in a Ca2+-dependent manner
    Kensuke Oikawa
    Department of Pathology, School of Nursing, Asahikawa Medical College, Midorigaoka Higashi 2 1, Asahikawa, Hokkaido 078 8510, Japan
    J Biol Chem 279:15142-52. 2004
    ..This report provides the first evidence of an interaction between visinin-like protein-3 and microsomal cytochrome b(5)...
  86. ncbi Topological changes in the CYP3A4 active site probed with phenyldiazene: effect of interaction with NADPH-cytochrome P450 reductase and cytochrome b5 and of site-directed mutagenesis
    Yoshitaka Yamaguchi
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas, USA
    Drug Metab Dispos 32:155-61. 2004
    ....
  87. ncbi Membrane properties induced by anionic phospholipids and phosphatidylethanolamine are critical for the membrane binding and catalytic activity of human cytochrome P450 3A4
    Keon Hee Kim
    Department of Genetic Engineering, Paichai University, Taejon 302 735, Korea
    Biochemistry 42:15377-87. 2003
    ..Taken together, it can be suggested that the chemical and physical properties of membranes induced by anionic phospholipids and PE are critical for the membrane binding and catalytic activity of CYP3A4...
  88. ncbi Effects of light and dark beer on hepatic cytochrome P-450 expression in male rats receiving alcoholic beverages as part of total enteral nutrition
    Mats Hidestrand
    Arkansas Children s Nutrition Center and University of Arkansas for Medical Sciences, Department of Pharmacology and Toxicology, Little Rock, Arkansas 72202, USA
    Alcohol Clin Exp Res 29:888-95. 2005
    ....
  89. ncbi Human liver microsomal cytochrome P-450 mephenytoin 4-hydroxylase, a prototype of genetic polymorphism in oxidative drug metabolism. Purification and characterization of two similar forms involved in the reaction
    T Shimada
    J Biol Chem 261:909-21. 1986
    ..Thus, at least two very similar forms of human P-450 are involved in S-mephenytoin 4-hydroxylation, an activity which shows genetic polymorphism...
  90. ncbi Dietary influence of selenium on the incidence of N-nitrosodiethylamine-induced hepatoma with reference to drug and glutathione metabolizing enzymes
    C Thirunavukkarasu
    Department of Medical Biochemistry, Dr ALM PG Institute of Basic Medical Sciences, University of Madras, Chennai 600 113, India
    Cell Biochem Funct 20:347-56. 2002
    ..Our results confirm the fact that selenium is particularly protective in limiting the action of DEN during the initiation phase of hepatocarcinogenesis...
  91. ncbi Biotransformation of praziquantel by human cytochrome p450 3A4 (CYP 3A4)
    Anna Godawska-Matysik
    Jagiellonian University, Medical College, Department of Chemical Technology and Biotechnology of Drugs, Medyczna 9, PL 30 688 Krakow, Poland
    Acta Pol Pharm 63:381-5. 2006
    ..In the second experiment microsomes isolated from Saccharomyces cerevisiae containing coexpressed human CYP 3A4, human CYP-reductase and human cytochrome b5 were used. The reactions were monitored by HPLC and MS...
  92. ncbi Human sterol 14alpha-demethylase activity is enhanced by the membrane-bound state of cytochrome b(5)
    D C Lamb
    Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, SY23 3DD, United Kingdom
    Arch Biochem Biophys 395:78-84. 2001
    ..This is the first report showing the stimulation of CYP51 by cytochrome b(5)...
  93. ncbi Structural and thermodynamic encoding in the sequence of rat microsomal cytochrome b(5)
    Juliette T J Lecomte
    Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA
    Biopolymers 89:428-42. 2008
    ..Structural information was obtained by nuclear magnetic resonance spectroscopy toward an atomic-level analysis of the effects...
  94. pmc Reductive activation of hexavalent chromium by human lung epithelial cells: generation of Cr(V) and Cr(V)-thiol species
    Griselda R Borthiry
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Inorg Biochem 102:1449-62. 2008
    ..In clonogenic assays, the cells were very sensitive to Na(2)CrO(4) and ZnCrO(4), but considerably less sensitive to PbCrO(4)...
  95. pmc Investigation of the mechanisms underlying the differential effects of the K262R mutation of P450 2B6 on catalytic activity
    Namandjé N Bumpus
    Department of Pharmacology, The University of Michigan, 1150 West Medical Center Drive, Ann Arbor, MI 48109 0632, USA
    Mol Pharmacol 74:990-9. 2008
    ..It is therefore proposed that the oxyferrous or iron-peroxo intermediate formed by the mutant enzyme in the presence of 17EE and efavirenz may be less stable than the same intermediates formed by the wild-type enzyme...
  96. ncbi Kinetics of testosterone 6beta-hydroxylation in the reconstituted system with similar ratios of purified CYP3A4, NADPH-cytochrome p450 oxidoreductase and cytochrome B5 to human liver microsomes
    M Taguchi
    Department of Biochemical Pharmacology and Toxicology, Faculty of Pharmaceutical Science, Chiba University, Japan
    Res Commun Mol Pathol Pharmacol 109:53-63. 2001
    ....
  97. ncbi Toxico-kinetics, recovery efficiency and microsomal changes following administration of deltamethrin to black Bengal goats
    S Juliet
    Department of Pharmacology and Toxicology, West Bengal University of Animal and Fishery Sciences 37, Kshudiram Bose Sarani, P.O.: Belgachia, Calcutta-700 037, India
    Pest Manag Sci 57:311-9. 2001
    ..Histopathological examination of liver, kidney, heart, spleen brain and lung sections of treated goats did not reveal any pathological changes...
  98. ncbi An enzymatically active chimeric protein containing the hydrophilic form of NADPH-cytochrome P450 reductase fused to the membrane-binding domain of cytochrome b5
    A A Gilep
    Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, Belarus
    Biochem Biophys Res Commun 284:937-41. 2001
    ..We conclude that differences on the surface of the P450s reflect unique and specific information essential for the recognition needed to establish reactions of intermolecular electron transfer from the flavoprotein CPR...
  99. ncbi Expression, purification, and physical properties of recombinant flavocytochrome fusion proteins containing rat cytochrome b(5) linked to NADPH-cytochrome P450 reductase by different membrane-binding segments
    A A Gilep
    Department of Biochemistry, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75235-9038, USA
    Arch Biochem Biophys 390:222-34. 2001
    ..The present study illustrates the application of artificial recombinant flavocytochromes as useful proteins for the study of intramolecular electron transport reactions for comparison with intermolecular interactions...
  100. ncbi Reconstitution of the enzymatic activities of cytochrome P450s using recombinant flavocytochromes containing rat cytochrome b(5) fused to NADPH--cytochrome P450 reductase with various membrane-binding segments
    A A Gilep
    Department of Biochemistry, Y7.326, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas, 75235-9038
    Arch Biochem Biophys 390:215-21. 2001
    ....
  101. ncbi Beta-naphthoflavone induction of a cytochrome P-450 arachidonic acid epoxygenase in chick embryo liver distinct from the aryl hydrocarbon hydroxylase and from phenobarbital-induced arachidonate epoxygenase
    K Nakai
    Department of Pharmacology, Cornell University Medical College, New York, New York 10021
    J Biol Chem 267:19503-12. 1992
    ..The findings support a physiologic role for P-450-induced arachidonate metabolism and provide a basis for a possible link between TCDD's induction of P-450 and alterations of cellular homeostasis...

Research Grants83

  1. ROLE OF HYDROXYLAMINE REDUCTION IN DRUG HYPERSENSITIVITY
    Lauren Trepanier; Fiscal Year: 2004
    ..abstract_text> ..
  2. ENZYMES AND REACTIONS FOR REPAIR OF DNA IN HUMAN CELLS
    THOMAS BRENT; Fiscal Year: 1993
    ..Expression of normal and modified P450s by use of transfection techniques will allow molecular interpretation of these changes in DMBA metabolism...
  3. Novel Mechanisms of Quinone Toxicity
    David Ross; Fiscal Year: 2010
    ..The studies will have broad applicability to a variety of organ systems. ..
  4. Prostaglandin 19-& 20-Hydroxylation by Cytochrome P450
    Bettie Sue Masters; Fiscal Year: 2005
    ..abstract_text> ..
  5. Nitogen reduction and xenobiotic response
    Lauren A Trepanier; Fiscal Year: 2010
    ..The ultimate goal of these studies is to find better ways to prevent these adverse reactions. ..
  6. Nitogen reduction and xenobiotic response
    Lauren Trepanier; Fiscal Year: 2007
    ..The ultimate goal of these studies is to find better ways to prevent these adverse reactions. ..
  7. Nitogen reduction and xenobiotic response
    Lauren Trepanier; Fiscal Year: 2009
    ..The ultimate goal of these studies is to find better ways to prevent these adverse reactions. ..
  8. ELECTRON TRANSFER PROCESSES IN IRON AND COPPER PROTEINS
    Harry Gray; Fiscal Year: 1990
    ..The proposed experiments will allow us to develop a better understanding of a fundamental process essential to all living organisms...
  9. CYTOCHROMES B: MODELS & PROTEIN STUDIES
    Frances Walker; Fiscal Year: 1991
    ..Native and site-directed mutagenetically modified cytochromes b5 will be utilized to investigate the effect of heme and axial ligand modifications on orbital energies and ..
  10. PARTICIPATION OF CYTOCHROME B5 IN ANESTHETIC METABOLISM
    Lucy Waskell; Fiscal Year: 2002
    ..Experiments are proposed which will also evaluate the specificity of this intramembrane binding interaction. ..
  11. OXYGEN SENSING AND SIGNAL TRANSDUCTION
    H Bunn; Fiscal Year: 2003
    ..abstract_text> ..
  12. ANDROGEN PRODUCTION IN ADRENARCHE AND PCOS
    WALTER MILLER; Fiscal Year: 2006
    ..abstract_text> ..
  13. Regulation of P450c17 Lyase Activity in Adrenache
    Qing Dong; Fiscal Year: 2007
    ..abstract_text> ..
  14. MOLECULAR PROBES OF THE MECHANISM OF CYTOCHROME P-450
    JOHN GROVES; Fiscal Year: 1993
    ..We plan also to study the DNA cleavage reactions of derivatives of cationic porphyrins with two metal coordinating appendages and to explore interactions of the species with ferritin mRNA...
  15. PARTICIPATION OF CYTOCHROME B5 IN ANESTHETIC METABOLISM
    Lucy Waskell; Fiscal Year: 2007
    ....