Genomes and Genes
Summary: Hemeproteins whose characteristic mode of action involves transfer of reducing equivalents which are associated with a reversible change in oxidation state of the prosthetic group. Formally, this redox change involves a single-electron, reversible equilibrium between the Fe(II) and Fe(III) states of the central iron atom (From Enzyme Nomenclature, 1992, p539). The various cytochrome subclasses are organized by the type of HEME and by the wavelength range of their reduced alpha-absorption bands.
Publications226 found, 100 shown here
- Respiration of Escherichia coli can be fully uncoupled via the nonelectrogenic terminal cytochrome bd-II oxidaseM Bekker
Swammerdam Institute for Life Sciences, BioCentrum, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
J Bacteriol 191:5510-7. 2009..A full understanding and control of this distribution open new avenues for optimization of biotechnological processes...
- Tracking the feeding patterns of tsetse flies (Glossina genus) by analysis of bloodmeals using mitochondrial cytochromes genesCatherine N Muturi
Molecular Biology and Biotechnology Department, International Centre of Insect Physiology and Ecology, Nairobi, Kenya
PLoS ONE 6:e17284. 2011....
- Oxygen reactivity of both respiratory oxidases in Campylobacter jejuni: the cydAB genes encode a cyanide-resistant, low-affinity oxidase that is not of the cytochrome bd typeRachel J Jackson
Department of Molecular Biology and Biotechnology, The University of Sheffield, Western Bank, Sheffield S10 2TN, UK
J Bacteriol 189:1604-15. 2007..These data are consistent with cytochrome oxidation during photolysis at low temperatures...
- Identification of the cydC locus required for expression of the functional form of the cytochrome d terminal oxidase complex in Escherichia coliC D Georgiou
J Bacteriol 169:2107-12. 1987..9 on the genetic linkage map, defining a new genetic locus, cydC. Evidence described in the text suggests that the gene product is probably required for the synthesis of the unique heme d component of the cytochrome d complex...
- The 'porin-cytochrome' model for microbe-to-mineral electron transferDavid J Richardson
Centre for Molecular and Structural Biochemistry, School of Biological Sciences and School of Chemistry, University of East Anglia, Norwich NR47TJ, UK
Mol Microbiol 85:201-12. 2012..It is debated how, once outside the cells, electrons are transferred from outer-membrane cytochromes to insoluble electron sinks...
- Effect of microaerophilic cell growth conditions on expression of the aerobic (cyoABCDE and cydAB) and anaerobic (narGHJI, frdABCD, and dmsABC) respiratory pathway genes in Escherichia coliC P Tseng
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90095, USA
J Bacteriol 178:1094-8. 1996..These two transcriptional regulators coordinate the hierarchial control of respiratory pathway gene expression in E. coli to ensure the optimal use of oxygen in the cell environment...
- Redox control of fast ligand dissociation from Escherichia coli cytochrome bdVitaliy B Borisov
Belozersky Institute of Physico Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russian Federation
Biochem Biophys Res Commun 355:97-102. 2007..As NO, an inhibitor of respiratory oxidases, is involved in the immune response against microbial infection, the rapid dissociation of NO from cytochrome bd may have important bearings on the patho-physiology of enterobacteria...
- Cytochrome bd confers nitric oxide resistance to Escherichia coliMaria G Mason
Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK
Nat Chem Biol 5:94-6. 2009..coli cells from NO-induced growth inhibition by virtue of its fast NO dissociation rate...
- The cytochrome bd quinol oxidase in Escherichia coli has an extremely high oxygen affinity and two oxygen-binding haems: implications for regulation of activity in vivo by oxygen inhibitionR D'Mello
Division of Life Sciences, King s College London, UK
Microbiology 142:755-63. 1996Cytochrome bd is a respiratory oxidase in Escherichia coli and many other bacteria. It contains cytochromes b558, b595 and d as redox centres, and is thus unrelated to the haem-copper super-family of terminal oxidases...
- Cytochrome bd biosynthesis in Escherichia coli: the sequences of the cydC and cydD genes suggest that they encode the components of an ABC membrane transporterR K Poole
Division of Life Sciences, King s College London, UK
Mol Microbiol 10:421-30. 1993..The CydDC system appears to be the first prokaryotic example of a heterodimeric ABC transport system in which each polypeptide contains both hydrophobic and ATP-binding domains...
- How cytochromes with different folds control heme redox potentialsJunjun Mao
Physics Department J 419, City College of New York, 138th Street and Convent Avenue, New York, New York 10031, USA
Biochemistry 42:9829-40. 2003The electrochemical midpoint potentials (E(m)'s) of 13 cytochromes, in globin (c, c(2), c(551), c(553)), four-helix bundle (c', b(562)), alpha beta roll (b(5)), and beta sandwich (f) motifs, with E(m)'s spanning 450 mV were calculated ..
- Interaction of bd-type quinol oxidase from Escherichia coli and carbon monoxide: heme d binds CO with high affinityV B Borisov
Department of Molecular Energetics of Microorganisms, Belozersky Institute of Physico Chemical Biology, Lomonosov Moscow State University, Moscow, Russia
Biochemistry (Mosc) 73:14-22. 2008..The apparent dissociation constant values for the heme d-CO complex of the membrane-bound and detergent-solubilized forms of the fully reduced enzyme are about 70 and 80 nM, respectively...
- Structural insights into the modulation of the redox properties of two Geobacter sulfurreducens homologous triheme cytochromesLeonor Morgado
REQUIMTE CQFB, Departamento de Quimica, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, Campus Caparica, 2829 516 Caparica, Portugal
Biochim Biophys Acta 1777:1157-65. 2008..The origins of the observed differences are discussed...
- Mutations affecting the cytochrome d-containing oxidase complex of Escherichia coli K12: identification and mapping of a fourth locus, cydDR K Poole
Microbial Physiology Research Group, Biosphere Sciences Division, King s College London, UK
J Gen Microbiol 135:1865-74. 1989..by nitrosoguanidine mutagenesis, lacks the spectroscopically detectable components of this oxidase, namely cytochromes b558, b595 and d...
- Cytochrome o (cyoABCDE) and d (cydAB) oxidase gene expression in Escherichia coli is regulated by oxygen, pH, and the fnr gene productP A Cotter
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024 1489
J Bacteriol 172:6333-8. 1990..We also examined cyoABCDE and cydAB expression in response to growth on alternative carbon compounds and to changes in the culture medium pH and osmolarity...
- The oxygen affinity of cytochrome bo' in Escherichia coli determined by the deoxygenation of oxyleghemoglobin and oxymyoglobin: Km values for oxygen are in the submicromolar rangeR D'Mello
Division of Life Sciences, King s College London, United Kingdom
J Bacteriol 177:867-70. 1995..Two Km values (0.15 to 0.35 microM and 0.016 to 0.085 microM) were detected, well below values determined for the purified oxidase by insensitive electrode methods...
- Antibody recognition force microscopy shows that outer membrane cytochromes OmcA and MtrC are expressed on the exterior surface of Shewanella oneidensis MR-1Brian H Lower
School of Environment and Natural Resources, The Ohio State University, 210 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, USA
Appl Environ Microbiol 75:2931-5. 2009..OmcA was localized to the interface between the cell and mineral. MtrC displayed a more uniform distribution across the cell surface. Both cytochromes were associated with an extracellular polymeric substance.
- Cloning and mutagenesis of genes encoding the cytochrome bd terminal oxidase complex in Azotobacter vinelandii: mutants deficient in the cytochrome d complex are unable to fix nitrogen in airM J Kelly
Division of Biosphere Sciences, Kings College London, United Kingdom
J Bacteriol 172:6010-9. 1990..Mutations in the cydB-hybridizing region resulted in the loss of spectral features associated with cytochromes b595 and d. A new locus, cydB, encoding cytochromes b595 and d in A. vinelandii is proposed...
- Requirement for terminal cytochromes in generation of the aerobic signal for the arc regulatory system in Escherichia coli: study utilizing deletions and lac fusions of cyo and cydS Iuchi
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
J Bacteriol 172:6020-5. 1990....
- Cytochrome bd-I in Escherichia coli is less sensitive than cytochromes bd-II or bo'' to inhibition by the carbon monoxide-releasing molecule, CORM-3: N-acetylcysteine reduces CO-RM uptake and inhibition of respirationHelen E Jesse
Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield, S10 2TN, UK
Biochim Biophys Acta 1834:1693-703. 2013....
- Rapid electron exchange between surface-exposed bacterial cytochromes and Fe(III) mineralsGaye F White
Centre for Molecular and Structural Biochemistry, School of Biological Sciences, and School of Chemistry, University of East Anglia, Norwich NR4 7TJ, United Kingdom
Proc Natl Acad Sci U S A 110:6346-51. 2013The mineral-respiring bacterium Shewanella oneidensis uses a protein complex, MtrCAB, composed of two decaheme cytochromes, MtrC and MtrA, brought together inside a transmembrane porin, MtrB, to transport electrons across the outer ..
- RO4938581, a GABAAα5 modulator, displays strong CYP1A2 autoinduction properties in ratsChristoffer Bundgaard
H Lundbeck A S, Discovery DMPK, Copenhagen, Valby, Denmark
Biochem Pharmacol 85:1363-9. 2013..Together, these findings suggest that RO4938581 possesses potent CYP1A2 autoinductive properties in rats and may serve as a tool for mechanistic metabolism or drug-drug interaction studies encircling this enzyme in rats...
- Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopyNadezda A Brazhe
Biological Faculty, Moscow State University, Russia
PLoS ONE 7:e41990. 2012This paper presents a nonivasive approach to study redox state of reduced cytochromes c, c1 and b of complexes II and III in mitochondria of live cardiomyocytes by means of Raman microspectroscopy...
- Cytochromes P460 and c'-beta; a new family of high-spin cytochromes cBradley O Elmore
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, St Paul, MN, USA
FEBS Lett 581:911-6. 2007b>Cytochromes-P460 of Nitrosomonas europaea and Methylococcus capsulatus (Bath), and the cytochrome c' of M...
- Biogenesis of respiratory cytochromes in bacteriaL Thony-Meyer
Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH Zentrum, Zurich, Switzerland
Microbiol Mol Biol Rev 61:337-76. 1997Biogenesis of respiratory cytochromes is defined as consisting of the posttranslational processes that are necessary to assemble apoprotein, heme, and sometimes additional cofactors into mature enzyme complexes with electron transfer ..
- Outer-membrane cytochrome-independent reduction of extracellular electron acceptors in Shewanella oneidensisClemens Bücking
Institut für Angewandte Biowissenschaften, Angewandte Biologie, Karlsruher Institut fur Technologie, Fritz Haber Weg 2, D 76131 Karlsruhe, Germany
Microbiology 158:2144-57. 2012..The final reduction is catalysed by outer-membrane cytochromes that transfer respiratory electrons either directly to mineral surfaces and metal ions bound in larger organic ..
- Purification of a cytochrome bd terminal oxidase encoded by the Escherichia coli app locus from a delta cyo delta cyd strain complemented by genes from Bacillus firmus OF4M G Sturr
Department of Biochemistry, Mount Sinai School of Medicine, City University of New York, USA
J Bacteriol 178:1742-9. 1996..In addition, cbdAB is suggested as a more appropriate gene designation for cytochrome bd than either appBC or cyxAB...
- Role of outer-membrane cytochromes MtrC and OmcA in the biomineralization of ferrihydrite by Shewanella oneidensis MR-1C L Reardon
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA
Geobiology 8:56-68. 2010..at the microbe-mineral interface, Shewanella oneidensis MR-1 mutants with in-frame deletions of outer-membrane cytochromes (OMCs), MtrC and OmcA, were characterized for the ability to reduce ferrihydrite (FH) using a suite of ..
- Electron flow in multiheme bacterial cytochromes is a balancing act between heme electronic interaction and redox potentialsMarian Breuer
Department of Physics and Astronomy, University College London, London WC1E 6BT, United Kingdom
Proc Natl Acad Sci U S A 111:611-6. 2014..To perform this function efficiently, microorganisms have adapted multiheme c-type cytochromes to arrange heme cofactors into wires that cooperatively span the cellular envelope, transmitting electrons ..
- Redox-dependent conformational changes in eukaryotic cytochromes revealed by paramagnetic NMR spectroscopyAlexander N Volkov
Jean Jeener NMR Centre, Structural Biology Brussels, Vrije Universiteit Brussel, Belgium
J Biomol NMR 52:245-56. 2012..We propose that the observed discrepancies arise from the changes in protein dynamics and discuss possible functional implications...
- Arsenite oxidase from Ralstonia sp. 22: characterization of the enzyme and its interaction with soluble cytochromesAurélie Lieutaud
Laboratoire de Bioénergétique et Ingénierie des Protéines UPR 9036, IFR88, CNRS, F 13402 Marseille Cedex 20, France
J Biol Chem 285:20433-41. 2010..The biochemical study of the enzyme demonstrates that it can accommodate in vitro various cytochromes, two of which, c(552) and c(554,) are from the parent species...
- Differential inducing effect of benzo[a]pyrene on gene expression and enzyme activity of cytochromes P450 1A1 and 1A2 in Sprague-Dawley and Wistar ratsMaura Floreani
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy
Drug Metab Pharmacokinet 27:640-52. 2012..was to compare RT-PCR, Western blot and determination of enzyme activity in the assessment of the induction of cytochromes P450 (CYPs) 1A1 and 1A2 by benzo[a]pyrene (BaP) in Sprague-Dawley and Wistar rats...
- Spectral analysis of cytochromes in rat heart myocytes: transient and steady-state photodiode array spectrophotometry measurementsP Sarti
Department of Biochemical Sciences, University of Rome La Sapienza, Italy
Arch Biochem Biophys 299:8-14. 1992..in resolving statically and kinetically, in the millisecond time region, the spectral contributions of the cytochromes. Spectral analysis was improved by adding carbon monoxide at concentrations which did not affect cytochrome c ..
- Involvement and specificity of Shewanella oneidensis outer membrane cytochromes in the reduction of soluble and solid-phase terminal electron acceptorsClemens Bücking
Department of Microbiology, Institute for Biology II, University of Freiburg, Germany
FEMS Microbiol Lett 306:144-51. 2010The formation of outer membrane (OM) cytochromes seems to be a key step in the evolution of dissimilatory iron-reducing bacteria...
- Escitalopram (S-citalopram) and its metabolites in vitro: cytochromes mediating biotransformation, inhibitory effects, and comparison to R-citalopramL L von Moltke
Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, 136 Harrison Ave, Boston, MA 02111, USA
Drug Metab Dispos 29:1102-9. 2001..S-DCT), and of S-DCT to S-didesmethyl-CT (S-DDCT), was studied in human liver microsomes and in expressed cytochromes (CYPs). Biotransformation of the R-enantiomer (R-CT) was studied in parallel...
- The role of multihaem cytochromes in the respiration of nitrite in Escherichia coli and Fe(III) in Shewanella oneidensisThomas A Clarke
Centre for Molecular and Structural Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
Biochem Soc Trans 36:1005-10. 2008..coli and the extracellular Fe(III) reductase system from Shewanella oneidensis contain multihaem c-type cytochromes as electron carriers and terminal reductases...
- Characterization of the Shewanella oneidensis MR-1 decaheme cytochrome MtrA: expression in Escherichia coli confers the ability to reduce soluble Fe(III) chelatesKaty E Pitts
Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences and School of Chemical Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
J Biol Chem 278:27758-65. 2003..Growth under these conditions results in the de novo synthesis of a number of periplasmic c-type cytochromes, many of which are multiheme in nature and are thought to be involved in the Fe(III) respiratory process...
- Direct involvement of type II secretion system in extracellular translocation of Shewanella oneidensis outer membrane cytochromes MtrC and OmcALiang Shi
Microbiology Group, Pacific Northwest National Laboratory, Richland, Washington 99354, USA
J Bacteriol 190:5512-6. 2008....
- Kinetics of reduction of Fe(III) complexes by outer membrane cytochromes MtrC and OmcA of Shewanella oneidensis MR-1Zheming Wang
Pacific Northwest National Laboratory, Richland, WA 99352, USA
Appl Environ Microbiol 74:6746-55. 2008Because of their cell surface locations, the outer membrane c-type cytochromes MtrC and OmcA of Shewanella oneidensis MR-1 have been suggested to be the terminal reductases for a range of redox-reactive metals that form poorly soluble ..
- Enzymatic reduction of chromate: comparative studies using sulfate-reducing bacteria. Key role of polyheme cytochromes c and hydrogenasesC Michel
, UPR 9036, CNRS, Institut de Biologie Structurale et Microbiologie, Marseille, France
Appl Microbiol Biotechnol 55:95-100. 2001..The ability of isolated polyheme cytochromes c from sulfate- and sulfur-reducing bacteria to reduce chromate was also analyzed. Tetraheme cytochrome c3(Mr...
- Flavohemoglobin Hmp affords inducible protection for Escherichia coli respiration, catalyzed by cytochromes bo' or bd, from nitric oxideT M Stevanin
Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom
J Biol Chem 275:35868-75. 2000..The results demonstrate the role of Hmp in protecting respiration from NO stress and are discussed in relation to the energy metabolism of E. coli in natural O(2)-depleted environments...
- Low-temperature EPR and Mössbauer spectroscopy of two cytochromes with His-Met axial coordination exhibiting HALS signalsGiorgio Zoppellaro
Department of Molecular Biosciences, University of Oslo, Box 1041 Blindern, 0316 Oslo, Norway
Chemphyschem 7:1258-67. 2006C-type cytochromes with histidine-methionine (His-Met) iron coordination play important roles in electron-transfer reactions and in enzymes...
- Fractionation of cytochromes of phototrophically grown Chloroflexus aurantiacus. Is there a cytochrome bc complex among them?Mikhail F Yanyushin
Institute of Basic Biological Problems, Pushchino, 142 290, Moscow Region, Russia
FEBS Lett 512:125-8. 2002..Two novel multisubunit complexes, similar to each other, with two heme c-bearing subunits were also purified...
- Nucleotide sequence of the heme subunit of flavocytochrome c from the purple phototrophic bacterium, Chromatium vinosum. A 2.6-kilobase pair DNA fragment contains two multiheme cytochromes, a flavoprotein, and a homolog of human ankyrinM M Dolata
Department of Biochemistry, University of Arizona, Tucson 85721
J Biol Chem 268:14426-31. 1993..Our results on the location of the enzyme are incompatible with this function...
- Enterococcus faecalis V583 contains a cytochrome bd-type respiratory oxidaseL Winstedt
Department of Microbiology, Lund University, Lund, Sweden
J Bacteriol 182:3863-6. 2000..Our results indicate that E. faecalis V583 cells have the capacity of aerobic respiration when grown in the presence of heme...
- Bis-histidine-coordinated hemes in four-helix bundles: how the geometry of the bundle controls the axial imidazole plane orientations in transmembrane cytochromes of mitochondrial complexes II and III and related proteinsEdward A Berry
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
J Biol Inorg Chem 13:481-98. 2008....
- Low-spin ferriheme models of the cytochromes: correlation of molecular structure with EPR spectral typeLiliya A Yatsunyk
Department of Chemistry, University of Arizona, Tucson, Arizona 85721 0041, USA
J Am Chem Soc 125:15986-6005. 2003..On the basis of this and previous work, the change from "large g(max)" to normal rhombic EPR signal occurs between axial ligand plane dihedral angles of 70 degrees and 30 degrees...
- The good, the bad and the ugly in oxygen-sensing: ROS, cytochromes and prolyl-hydroxylasesTill Acker
Neurologisches Institut, Edinger Institut, Klinikum der J W Goethe Universität Frankfurt am Main, Deutschordenstr 46, D 60528 Frankfurt, Germany
Cardiovasc Res 71:195-207. 2006..Herein, we propose an integrated model for these various oxygen-sensing mechanisms that very efficiently regulate HIF-alpha activity and plasma membrane potassium-channel (PMPC) conductivity...
- The outer membrane cytochromes of Shewanella oneidensis MR-1 are lipoproteinsC R Myers
Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Lett Appl Microbiol 39:466-70. 2004To determine if the outer membrane (OM) cytochromes OmcA and OmcB of the metal-reducing bacterium Shewanella oneidensis MR-1 are lipoproteins, and to assess cell surface exposure of the cytochromes by radioiodination.
- Identification of 42 possible cytochrome C genes in the Shewanella oneidensis genome and characterization of six soluble cytochromesTerry E Meyer
Department of Biochemistry and Molecular Biophysics University of Arizona, Tucson, Arizona 85721, USA
OMICS 8:57-77. 2004..However, we found only six soluble cytochromes c in extracts of S...
- Overlapping role of the outer membrane cytochromes of Shewanella oneidensis MR-1 in the reduction of manganese(IV) oxideJ M Myers
Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Lett Appl Microbiol 37:21-5. 2003To determine if the outer membrane (OM) cytochromes OmcA and OmcB of the metal-reducing bacterium Shewanella oneidensis MR-1 have distinct or overlapping roles in the reduction of insoluble manganese(IV) oxide.
- Potential role of a novel psychrotolerant member of the family Geobacteraceae, Geopsychrobacter electrodiphilus gen. nov., sp. nov., in electricity production by a marine sediment fuel cellDawn E Holmes
Department of Microbiology, 106N Morrill IV N, University of Massachusetts, Amherst, MA 01003, USA
Appl Environ Microbiol 70:6023-30. 2004..They are gram-negative, nonmotile rods, with abundant c-type cytochromes. Phylogenetic analysis of the 16S rRNA, recA, gyrB, fusA, rpoB, and nifD genes indicated that strains A1(T) and ..
- Sequence conservation in families whose members have little or no sequence similarity: the four-helical cytokines and cytochromesEmma E Hill
MRC Laboratory of Molecular Biology, Cambridge, UK
J Mol Biol 322:205-33. 2002..questions we have analysed the structures and sequences of two superfamilies: the four-helical cytokines and cytochromes c'-b(562). Members of these superfamilies have sequence similarities that are either very low or not detectable...
- Extracellular electron transfer: wires, capacitors, iron lungs, and moreDerek R Lovley
Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA
Geobiology 6:225-31. 2008
- Vanadium(V) reduction by Shewanella oneidensis MR-1 requires menaquinone and cytochromes from the cytoplasmic and outer membranesJudith M Myers
Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
Appl Environ Microbiol 70:1405-12. 2004..This is the first report delineating specific electron transport components that are required for V(V) reduction and of a role for OM cytochromes in the reduction of a soluble metal species.
- Bacillus subtilis contains two small c-type cytochromes with homologous heme domains but different types of membrane anchorsJ Bengtsson
Department of Microbiology, Lund University, Sölvegatan 12, S 223 62 Lund, Sweden
J Biol Chem 274:26179-84. 1999..Thus, B. subtilis contains two small, very similar, c-type cytochromes with different types of membrane anchors...
- DNA microarray and proteomic analyses of the RpoS regulon in Geobacter sulfurreducensCinthia Núñez
Departamento de Microbiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Av Universidad 2001, Col Chamilpa Cuernavaca, Morelos 62210, Mexico
J Bacteriol 188:2792-800. 2006..sulfurreducens growth using oxygen as an electron acceptor, and expression of at least 13 c-type cytochromes, including one previously shown to participate in Fe(III) reduction (MacA), were RpoS dependent...
- Pressure-regulated biosynthesis of cytochrome bd in piezo- and psychrophilic deep-sea bacterium Shewanella violacea DSS12Hideyuki Tamegai
Department of Chemistry, College of Humanities and Sciences, Nihon University, 3 25 40 Sakurajosui, Setagaya Ku, Tokyo 156 8550, Japan
Extremophiles 9:247-53. 2005..These results suggested that the significant amount of d-type cytochrome expression is specific event under the growth condition of high pressure...
- Models of the cytochromes: crystal structures and EPR spectral characterization of low-spin bis-imidazole complexes of (OETPP)Fe(III) having intermediate ligand plane dihedral anglesLiliya A Yatsunyk
Department of Chemistry, University of Arizona, Tucson, Arizona 85721 0041, USA
Inorg Chem 45:5417-28. 2006..However, in comparison to several proteins with dihedral angles near 60 degrees and Type II EPR spectra, we may conclude that the switch in EPR signal type occurs near 57 degrees +/- 3-5 degrees...
- The anticancer drug ellipticine is a potent inducer of rat cytochromes P450 1A1 and 1A2, thereby modulating its own metabolismDagmar Aimová
Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, 128 40 Prague 2, Czech Republic
Drug Metab Dispos 35:1926-34. 2007..mainly on DNA intercalation, inhibition of topoisomerase II, and formation of covalent DNA adducts mediated by cytochromes P450 (P450s) and peroxidases...
- Electrochemical interrogations of the Mtr cytochromes from Shewanella: opening a potential windowMackenzie Firer-Sherwood
Department of Chemistry, Boston University, 590 Commonwealth Ave, Boston, MA 02215, USA
J Biol Inorg Chem 13:849-54. 2008The multi-heme cytochromes from Shewanella oneidensis associated with the dissimilatory metal reduction (DMR) pathway have been investigated using the technique of protein film voltammetry (PFV)...
- Effects of drought stress and subsequent rewatering on photosynthetic and respiratory pathways in Nicotiana sylvestris wild type and the mitochondrial complex I-deficient CMSII mutantAlexander Galle
Grup de Recerca en Biologia de les Plantes en Condicions Mediterranies, Departament de Biologia UIB IMEDEA, Universitat de les Illes Balears, Carretera de Valldemossa Km 7 5, E 07122 Palma de Mallorca, Spain
J Exp Bot 61:765-75. 2010....
- Aerobic regulation of cytochrome d oxidase (cydAB) operon expression in Escherichia coli: roles of Fnr and ArcA in repression and activationP A Cotter
Department of Microbiology and Molecular Genetics and the Molecular Biology Institute, University of California, Los Angeles 90095 1489, USA
Mol Microbiol 25:605-15. 1997..Based on the results of the in vitro and in vivo studies, a working model for ArcA activation and Fnr repression of cydAB transcription is proposed...
- Models of the bis-histidine-ligated electron-transferring cytochromes. Comparative geometric and electronic structure of low-spin ferro- and ferrihemesF Ann Walker
Department of Chemistry, University of Arizona, Tucson, Arizona 85721 0041, USA
Chem Rev 104:589-615. 2004
- Terminal oxidases of cyanobacteriaS E Hart
Department of Biochemistry, University of Cambridge, Building O, The Downing Site, Cambridge CB2 1QW, U K
Biochem Soc Trans 33:832-5. 2005..We summarize here their genetic, biochemical and biophysical characterization to date and discuss their interactions with electron donors as well as their physiological roles...
- The quinol:fumarate oxidoreductase from the sulphate reducing bacterium Desulfovibrio gigas: spectroscopic and redox studiesRita S Lemos
Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
J Bioenerg Biomembr 34:21-30. 2002..Furthermore, co-reconstitution in liposomes of D. gigas FRD, duroquinone, and D. gigas cytochrome bd shows that this system is capable of coupling succinate oxidation with oxygen reduction to water...
- Interactions between the cytochrome pathway and the alternative oxidase in isolated Acanthamoeba castellanii mitochondriaWieslawa Jarmuszkiewicz
Department of Bioenergetics, Adam Mickiewicz University, Institute of Molecular Biology and Biotechnology, Poznan, Poland
J Bioenerg Biomembr 34:31-40. 2002..These unexpected effects of BHAM and KCN observed at a given Q reduction level are likely due to functional connections between the two pathways activities or to protein-protein interaction...
- Electron transport routes in whole cells of Synechocystis sp. strain PCC 6803: the role of the cytochrome bd-type oxidaseStephan Berry
Lehrstuhl für Biochemie der Pflanzen, Fakultat fur Biologie, Ruhr Universitat Bochum, D 44780 Bochum, Germany
Biochemistry 41:3422-9. 2002..In contrast, no indication of quinol oxidase activity in thylakoids was found for a second alternative oxidase encoded by the ctaII genes...
- Substrate kinetics of the Acanthamoeba castellanii alternative oxidase and the effects of GMPWieslawa Jarmuszkiewicz
Laboratory of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61 701 Poznan, Poland
Biochim Biophys Acta 1708:71-8. 2005..Thus, the apparent affinity of alternative oxidase for oxygen decreased when the ubiquinone reduction level increased either by changing the activator or the respiratory substrate availability...
- FTIR spectroscopic evidence for the involvement of an acidic residue in quinone binding in cytochrome bd from Escherichia coliJie Zhang
Department of Biochemistry, University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
Biochemistry 41:4612-7. 2002....
- Effect of temperature on rates of alternative and cytochrome pathway respiration and their relationship with the redox poise of the quinone poolOwen K Atkin
Department of Plant Sciences, University of Adelaide, South Australia 5005, Australia
Plant Physiol 128:212-22. 2002....
- Oxygen deficiency responsive gene expression in Chlamydomonas reinhardtii through a copper-sensing signal transduction pathwayJeanette M Quinn
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 1569, USA
Plant Physiol 128:463-71. 2002..A Crr1-independent pathway for Hyd1 expression in oxygen-depleted C. reinhardtii demonstrates the existence of multiple oxygen/redox-responsive circuits in this model organism...
- Stress-induced changes in ubiquinone concentration and alternative oxidase in plant mitochondriaV N Popov
Department of Plant Physiology and Biochemistry, Voronezh State University, Russia
Biosci Rep 21:369-79. 2001..The role of reduced Q accumulation and AOX induction in the defense against oxidative damage is discussed...
- A bacterial glutathione transporter (Escherichia coli CydDC) exports reductant to the periplasmMarc S Pittman
Department of Molecular Biology and Biotechnology, Firth Court, The University of Sheffield, UK
J Biol Chem 280:32254-61. 2005..These results identify the first prokaryotic GSH transporter and indicate a key role for GSH in periplasmic redox homeostasis...
- Intramolecular electron transfer in cytochrome cd(1) nitrite reductase from Pseudomonas stutzeri; kinetics and thermodynamicsOle Farver
Department of Analytical and Pharmaceutical Chemistry, The Royal Danish School of Pharmacy, DK 2100 Copenhagen, Denmark
Biophys Chem 98:27-34. 2002..The significance of these parameters obtained at low degree of reduction of the enzyme is discussed and compared with earlier studies on cd(1) nitrite reductases from other sources...
- QO site deficiency can be compensated by extragenic mutations in the hinge region of the iron-sulfur protein in the bc1 complex of Saccharomyces cerevisiaeGael Brasseur
Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Biol Chem 279:24203-11. 2004....
- Arginine 391 in subunit I of the cytochrome bd quinol oxidase from Escherichia coli stabilizes the reduced form of the hemes and is essential for quinol oxidase activityJie Zhang
Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA
J Biol Chem 279:53980-7. 2004..The fact that the midpoint potentials of heme d and heme b(595) are also significantly lowered in the R391A mutant is consistent with these hemes being physically close together on the periplasmic side of the membrane...
- Proton NMR study of the heme environment in bacterial quinol oxidasesJie Zhang
Department of Biochemistry, University of Illinois, 600 South Mathews Avenue, Urbana, IL 61801, USA
Arch Biochem Biophys 421:186-91. 2004..The results presented here demonstrate that structural changes of one metal center can significantly influence the structural properties of other nearby metal center(s) in large membrane paramagnetic metalloproteins...
- Modeling electron transfer thermodynamics in protein complexes: interaction between two cytochromes c(3)Vitor H Teixeira
Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
Biophys J 86:2773-85. 2004Redox protein complexes between type I and type II tetraheme cytochromes c(3) from Desulfovibrio vulgaris Hildenborough are here analyzed using theoretical methodologies...
- Evidence for two different electron transfer pathways in the same enzyme, nitrate reductase A from Escherichia coliRoger Giordani
Laboratoire de Chimie Bacterienne, Institut Fédératif Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, Marseille, France
Eur J Biochem 271:2400-7. 2004..These results, and those of spectrophotometric studies of a mutant lacking the highest-potential [Fe-S] cluster, allow us to propose a two-pathway electron transfer model for nitrate reductase A from Escherichia coli...
- Synthetic models for heme-copper oxidasesEunSuk Kim
Department of Chemistry, Johns Hopkins University, Charles and 34th Streets, Baltimore, Maryland 21218, USA
Chem Rev 104:1077-133. 2004
- Detection of mitochondrial electron chain carrier redox status by transhepatic light intensity during rat liver reperfusionP Dutkowski
Department of Surgery, University of Mainz, Langenbeckstr 1, 55101, Mainz, Germany
Cryobiology 47:125-42. 2003..Transhepatic photometric analysis allowed determination of the redox status of mitochondrial cytochromes during preservation, rewarming and reperfusion...
- [The alternative oxidase of Yarrowia lipolytica mitochondria is unable to compete with the cytochrome pathway for electrons]V K Akimenko
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr Nauki 5, Pushchino, Moscow Oblast, 142290 Russia
Mikrobiologiia 72:453-8. 2003..These findings indicate that the alternative pathway is unable to compete with the cytochrome respiratory chain for electrons. The alternative pathway transfers only electrons that are superfluous for the cytochrome chain...
- Resonance Raman detection of the Fe2+-C-N modes in heme-copper oxidases: a probe of the active siteEftychia Pinakoulaki
Department of Chemistry, University of Crete, 71409 Heraklion, Crete, Greece
Inorg Chem 43:4907-10. 2004....
- Genetic evidence for a regulatory pathway controlling alternative oxidase production in Neurospora crassaAndrea T Descheneau
Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
Genetics 169:123-35. 2005..The discovery that several genes are required for regulation of aod-1 suggests the existence of a complex pathway for signaling from the mitochondria to the nucleus and/or for expression of the gene...
- Contribution of proton-translocating proteins to the virulence of Salmonella enterica serovars Typhimurium, Gallinarum, and Dublin in chickens and miceA K Turner
Institute for Animal Health, Compton Laboratory, Newbury, Berkshire RG20 7NN, United Kingdom
Infect Immun 71:3392-401. 2003..The data support the targeting of respiratory components for the production of live vaccines and suggest that mutations in the atp operon provide suitable candidates for broad-spectrum attenuation of a range of Salmonella serovars...
- Time-resolved electrometric and optical studies on cytochrome bd suggest a mechanism of electron-proton coupling in the di-heme active siteIlya Belevich
Helsinki Bioenergetics Group, Institute of Biotechnology, University of Helsinki, PB 65 Viikinkaari 1, FIN 00014 Helsinki, Finland
Proc Natl Acad Sci U S A 102:3657-62. 2005..Presumably, E445 is one of the two redox-linked ionizable groups required for charge compensation of the di-heme oxygen-reducing site (b(595), d) upon its full reduction by two electrons...
- Cytochrome bd oxidase, oxidative stress, and dioxygen tolerance of the strictly anaerobic bacterium Moorella thermoaceticaAmaresh Das
Department of Chemistry and Molecular Biology, University of Georgia, Athens, GA 30602 2556, USA
J Bacteriol 187:2020-9. 2005..The combined evidence indicates that cytochrome bd oxidase and cysteine synthase protect against oxidative stress and contribute to the limited dioxygen tolerance of M. thermoacetica...
- Knockout of the high-coupling cytochrome aa3 oxidase reduces TCA cycle fluxes in Bacillus subtilisNicola Zamboni
Institute of Biotechnology, ETH Zurich, CH 8093, Zurich, Switzerland
FEMS Microbiol Lett 226:121-6. 2003..Since the product-corrected biomass yields were identical in mutants and parent, the results show that efficient ATP generation is not overly important for exponential growth of B. subtilis in batch culture...
- Intracellular expression of Vitreoscilla hemoglobin modifies microaerobic Escherichia coli metabolism through elevated concentration and specific activity of cytochrome oPhilip S Tsai
Institute of Biotechnology, ETH Zurich, Switzerland
Biotechnol Bioeng 79:558-67. 2002..Results suggest that the expression of VHb in E. coli increases the level and activity of terminal oxidases and thereby improves the efficiency of microaerobic respiration and growth...
- The nature of the exchange coupling between high-spin Fe(III) heme o3 and CuBII in Escherichia coli quinol oxidase, cytochrome bo3: MCD and EPR studiesMyles R Cheesman
School of Chemical Sciences and Pharmacy, Centre for Metalloprotein Spectroscopy and Biology CMSB, University of East Anglia, Norwich NR4 7TJ, U K
J Am Chem Soc 126:4157-66. 2004..These conclusions overturn a long-held view that such EPR signals in bovine cytochrome c oxidase arise from an S' = 2 ground state resulting from strong exchange coupling (J > 10(2) cm-1) within the active site...
- Electrostatic analysis and Brownian dynamics simulation of the association of plastocyanin and cytochrome fF De Rienzo
, Dipartimento di Chimica, Via Campi, 183-41100 Modena, Italy
Biophys J 81:3090-104. 2001....
- The nucleotide sequence of the cyd locus encoding the two subunits of the cytochrome d terminal oxidase complex of Escherichia coliG N Green
Department of Biochemistry, University of Illinois, Urbana 61801
J Biol Chem 263:13138-43. 1988..There are only 10 histidines in both subunits, several of which are likely to serve as heme axial ligands...
- Glutamates 99 and 107 in transmembrane helix III of subunit I of cytochrome bd are critical for binding of the heme b595-d binuclear center and enzyme activityTatsushi Mogi
Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259, Midori ku, Yokohama 226 8503, Japan
Biochemistry 45:15785-92. 2006..On the basis of this study and previous studies, we propose arrangement of transmembrane helices in subunit I, which may explain possible roles of conserved hydrophilic residues within the membrane...
- Glutamate 107 in subunit I of the cytochrome bd quinol oxidase from Escherichia coli is protonated and near the heme d/heme b595 binuclear centerKe Yang
Department of Biochemistry, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
Biochemistry 46:3270-8. 2007..Mutagenesis shows that there are several acidic residues, including E99 and E107 as well as D29 (in CydB), which are important for the assembly or stability of the heme d/heme b595 active site...
- High-stability semiquinone intermediate in nitrate reductase A (NarGHI) from Escherichia coli is located in a quinol oxidation site close to heme bDPascal Lanciano
Unité de Bioénergétique et Ingénierie des Protéines UPR9036, Institut de Biologie Structurale et de Microbiologie, CNRS and Université d Aix Marseille, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Biochemistry 46:5323-9. 2007..Overall, our results indicate that the semiquinone is located within the quinol oxidation site QD. Details of the possible binding motif of the semiquinone and mechanistic implications are discussed...
- Discovery of the true peroxy intermediate in the catalytic cycle of terminal oxidases by real-time measurementIlya Belevich
Helsinki Bioenergetics Group, Institute of Biotechnology, University of Helsinki, Post Office Box 65 Viikinkaari 1, FI 00014 Helsinki, Finland
J Biol Chem 282:28514-9. 2007..The lifetime of the new intermediate is 47 micros; it decays into oxoferryl species due to oxidation of low spin heme b(558) that is linked to significant charge translocation across the membrane...
- Thermodynamic and kinetic characterisation of individual haems in multicentre cytochromes c3Catarina M Paquete
Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Rua da Quinta Grande, 6, Apt 127, 2780 156 Oeiras, Portugal
Biochim Biophys Acta 1767:1169-79. 2007..redox centres and allow the determination of the microscopic thermodynamic parameters in several multihaem cytochromes c(3) isolated from different sulphate-reducing bacteria...
- Heme-copper oxidases use tunneling pathwaysDavid N Beratan
Department of Chemistry, French Family Science Center, Duke University, Durham, NC 27708, USA
Proc Natl Acad Sci U S A 105:403-4. 2008
- Strong excitonic interactions in the oxygen-reducing site of bd-type oxidase: the Fe-to-Fe distance between hemes d and b595 is 10 AAlexander M Arutyunyan
A N Belozersky Institute of Physico Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia
Biochemistry 47:1752-9. 2008..The physical proximity supports the hypothesis that heme d and heme b595 can form a di-heme oxygen reducing site, a unique structure for respiratory oxidases...
- Investigation of the role of the cydD gene product in production of a functional cytochrome d oxidase in Escherichia coliK J Bebbington
Department of Biology, Imperial College of Science, Technology and Medicine, London, UK
FEMS Microbiol Lett 112:19-24. 1993..Therefore, if CydD has a role in haem d biosynthesis it must be in a unique step only associated with the synthesis of the haem d prosthetic group of cytochrome d...
- Roles of Nitric Oxide and Superoxide in CystitisANTHONY JOHN KANAI; Fiscal Year: 2013..Hypothesis: FA-NO2 are radioprotective by desensitizing TRPA1 and TRPV1 channels and BDNF-AB and NGF-AB are therapeutic by inhibiting nerve growth and sensitization in bladder and spinal cord. ..
- THE ROLE OF ACETYLATION IN MITOCHONDRIAL FUNCTIONGary Cecchini; Fiscal Year: 2013..Using the SIRT3 KO mouse model, it will be determined if the properties of cardiac tissue such as pre- and post-conditioning are altered by acetylation/deacetylation of proteins. ..
- Functional Genomics of the Mitochondrial ProteomeDennis R Winge; Fiscal Year: 2011..The function of up to 30% of the mitochondrial proteome is unknown. This proposal will elucidate the function of these un-annotated mitochondrial proteins, providing new insights into the role of mitochondria in human disease. ..
- Retinoid Metabolism and Alcohol Induced DiseaseWilliam S Blaner; Fiscal Year: 2010..alcohol accelerates retinoid oxidation, involving ethanol-inducible cytochromes like Cyp2E1;and 3. alcohol increases mobilization of stored retinoid from the liver to other tissues...
- Protein Nitration in the PlacentaLeslie Myatt; Fiscal Year: 2012....
- MOLECULAR MECHANISMS OF COMPLEX MIXTURE TOXICITYAlvaro Puga; Fiscal Year: 2013....
- Therapeutic Effect of a Novel Antioxidant on Degenerative Eye DisordersNuran Ercal; Fiscal Year: 2012..NACA eye drops would represent an alternative to costly cataract surgery, reduce health care costs related to age-related eye diseases and greatly improve the quality of life of people affected by these diseases. ..
- Role of Mitochondria in the Regulation of Iron metabolism by AlcoholDUYGU DEE DEE HARRISON-FINDIK; Fiscal Year: 2012..The knowledge obtained from these studies, characterizing the pivotal role of mitochondria, will enable us to determine the mechanisms of liver injury caused by iron and alcohol in patients with ALD. ..
- Genetic Studies of Optic AtrophyTaosheng Huang; Fiscal Year: 2012..This study will allow us to understand how mutations of OPA1 cause optic atrophy and explore its novel function in lifespan and provide us a potential opportunity to develop treatments for the disease. ..
- Microsomal Cytochromes P450 and their Interactions with their Redox PartnersLucy A Waskell; Fiscal Year: 2013....
- Role of Retroelements in Environmental AtherogenesisKenneth S Ramos; Fiscal Year: 2010..effects of benzo(a)pyrene (BaP), a carcinogen present in various forms of pollution and metabolized by cytochromes P450 to intermediates that damage DNA and cause oxidative stress...
- Protection of Brain Injury from Cyanide Poisoning by Carnosic AcidStuart A Lipton; Fiscal Year: 2013..To investigate neuroprotective effects of CA in an in vitro model of cyanide poisoning. Specific Aim 2. To examine neuroprotective effects of CA in an in vivo mouse model mimicking the effects of cyanide ingestion. ..
- Biochemical Characterization of Cytochrome b5 Reductase in Candida albicansMARY JOLENE PATRICIA HOLLOWAY; Fiscal Year: 2010..I will also learn new techniques used in biochemistry and proteomics, such as mass spectrometry, protein pull-down assays, and other methods involved in the characterization of proteins. ..
- Extracellular ubiquitin: role in myocyte apoptosis and myocardial remodelingKrishna Singh; Fiscal Year: 2010....
- Electron Transfer in Iron and Copper ProteinsJay R Winkler; Fiscal Year: 2013..Efforts will focus on oxidative generation of Compounds I and II in cytochromes P450 (Aim 3) and a bacterial enzyme that exhibits NOS-like reactivity (Aim 4).
- StAR expression: Integration of Transcription with regulation via the mRNA 3'UTRCOLIN ROBERT JEFCOATE; Fiscal Year: 2012..TIS11b regulation is present in other tissues that make steroids (testis). Deficiency in this mRNA regulator may lead to more sluggish responses to ACTH and stress as well as abnormal cortisol output. ..
- Prevention of Retinal Degeneration by Crystallins in Experimental UveitisNarsing A Rao; Fiscal Year: 2011..PUBLIC HEALTH RELEVANCE AlphaA crystallin is a unique protein present in the eye and other tissues. Lack of this protein can cause blindness, and supplement of this protein can prevent several blinding diseases. ..
- Nanoelectrode arrays for study of the molecular mechanisms, triggers, and inhibitPeter J Burke; Fiscal Year: 2012..A high throughput technology to monitor P in mitochondria will allow further studies of these issues in cancer biology. ..
- Functional roles of Nrf2 and NQO1 genetic variants in hyperoxic lung injury-ARDSBhagavatula Moorthy; Fiscal Year: 2013..g., use of PPI such as OM) for the prevention/treatment of ALI/ARDS in humans. ..
- Visualization of ototoxic mitochondrial dysfunction in mammalian hair cellsShelley A Batts; Fiscal Year: 2012..Thus, this work could fundamentally affect the way that hearing research is conducted by allowing longitudinal investigations into the intact mammalian cochlea for the first time. ..
- Early Disease Biomarkers of PCB-exposed Human PopulationSisir K Dutta; Fiscal Year: 2010....
- Influence of Heme on Hepatic Cytochrome P450 TurnoverMaria Almira Correia; Fiscal Year: 2013..causes global translational arrest of de novo synthesis of a myriad of hepatic proteins including cytochromes P450 (P450s), hemoprotein enzymes engaged in the breakdown and clearance of endo- and xenobiotics such as drugs,..
- Mitochondrial function and antifungal photodynamic therapyConstantine G Haidaris; Fiscal Year: 2011..abstract_text> ..
- TNF-alpha Signaling in Silica-Induced Lung FibrosisLuis A Ortiz; Fiscal Year: 2012..34 and 2.4 years for survival and graft rejection respectively). Consequently, these data indicate that silicosis still is a serious and frequently lethal lung disease for which no successful treatment is available. ..
- Non-Hodgkin lymphoma in women: reproductive, hormonal and genetic factorsPaige M Bracci; Fiscal Year: 2011....
- Mitochondrial Protection Within Chronically Ischemic MyocardiumEdward O McFalls; Fiscal Year: 2010..Using this model, we propose that a class of drugs used in diabetic patients that has been associated with heart failure exacerbations, alters the mitochondrial energy production in a similar way. ..
- HEPATIC DEGRADATION OF CYTOCHROME P450 ENZYMESMaria Almira Correia; Fiscal Year: 2013DESCRIPTION (provided by applicant): The hepatic cytochromes P450 (P450s) are endoplasmic-reticulum membrane-anchored enzymes engaged in the breakdown of endo- and xenobiotics i.e...
- NEWBORN IRON DEFICIENCYMichael K Georgieff; Fiscal Year: 2013..Iron plays a key, direct role in regulating mTOR activity through cytochromes and hypoxia inducible factor 1-alpha...
- Creation of hyperactive transposons for mutagenesis in rodentsEric M Ostertag; Fiscal Year: 2011..Thus, if successful, this project would benefit many goals of public health by making the production of mutations in the rat that model human diseases readily accessible to the research community. ..
- Activating liver carcinogens in yeast by expressing CYP450 polymorphismsMICHAEL THOMAS FASULLO; Fiscal Year: 2010..Ultimately, the information will aid public health practitioners and clinicians to identify which individuals are at highest risk for liver cancer, and guide prevention and early detection efforts. ..
- Role of Mitochondria Targeted CYP2E1 and HO-1 in Alcohol Mediated Tissue InjuryNarayan G Avadhani; Fiscal Year: 2012..The proposed research project is based on the hypothesis that mitochondrially targeted CYP2E1 and HO-1 contribute to ROS production, oxidative stress and mitochondrial dysfunction as important factors leading to tissue injury. ..
- Molecular &Cellular Effects of Human Mutations in Cytochrome P450 ReductaseSatya Prakash Panda; Fiscal Year: 2013..NADPH-cytochrome P450 oxidoreductase (POR) is the sole provider of electrons to the microsomal cytochromes P450 and to heme oxygenase, which catabolizes heme degradation...
- Genetic Determinants of the Response to Thienopyridine TherapyJessica L Mega; Fiscal Year: 2013..The TIMI Study Group also offers the appropriate training environment to provide Dr. Mega with the skills needed to lead the multicenter clinical trial described in Aim 3. ..
- Research Center for Pharmacology &Drug ToxicologyL Jackson Roberts; Fiscal Year: 2013....
- Targeting ATAP-mediated Apoptosis in Treatment of Prostate CancerZelin Sheng; Fiscal Year: 2013....
- Alcoholic Liver Dysfunction Potentiation by Hyperlipidemia and Cigarette SmokeSHANNON MARIE BAILEY; Fiscal Year: 2013....
- Human hepatocytes for drug toxicity screening from Cardiac Death Donor liversMark G Clemens; Fiscal Year: 2012..should allow us to quickly translate this innovative technology into a commercially viable product. ..
- Novel Catalases and their ProductsAlan R Brash; Fiscal Year: 2013..By uncovering the details of how different enzymes deal with oxidants and how they relate or differ from each other this project will improve our understanding of oxidative stress, which will ultimately help control it. ..
- The Role of Cyp2b in Toxicant Metabolism and SensitivityWilliam S Baldwin; Fiscal Year: 2012....
- Developing a prediction model for vincristine-induced peripheral neuropathyJamie L Renbarger; Fiscal Year: 2010..Once the correlation between genetics and vincristine neurotoxicity fully elucidated, it will be possible to individualize vincristine dosing using genetic information to optimize therapeutic efficacy while minimizing toxicity. ..
- Hepatic CYP enzymes and host-pathogen interactionsEdward T Morgan; Fiscal Year: 2013..This project will increase our understanding of how liver metabolism changes during infection or inflammation, and may discover new strategies to treat infectious and inflammatory diseases. ..
- PERINATAL MECHANISMS OF DETOXIFICATIONEric F Johnson; Fiscal Year: 2013..Additionally, we will address how genetic deficiencies of this capacity affect susceptibility to hypertension due to renal and liver dysfunction. ..
- Eicosanoids and Endothelial Cell DysfunctionGadiparthi N Rao; Fiscal Year: 2013....
- Role of The Jak-Stat Pathway in Ischemic PreconditioningYu Ting Xuan; Fiscal Year: 2010..This proposal will provide important new insights into the signaling and molecular mechanisms whereby the late phase of ischemic PC reprograms the heart in the intact animal. ..
- Genetic Susceptibility Factors in Porphyria Cutanea Tarda (PCT)Karl Anderson; Fiscal Year: 2009..This study and projects which follow will lead to a better understanding of PCT and also contribute to knowledge about how agents such as alcohol and hepatitis C interact with genetic factors in causing human liver diseases. ..
- Investigation of underlying mechanisms of sex differences in cerebral ischemiaChad S Siegel; Fiscal Year: 2010..The overall goal of this proposal is to investigate mechanisms underlying sex differences in ischemic cell death. ..
- NOVEL HIGH THROUGHPUT PLATFORM FOR SCREENING CYTOCHROME P450 INDUCTIONMARIA INES MORANO; Fiscal Year: 2013....
- CYP1B1, NF-kB , and Regulation of AngiogenesisTAMMY LYNN PALENSKI; Fiscal Year: 2012..A better understanding of how angiogenesis is regulated will aid in development of new and more effective therapies for these blinding diseases, as well as other diseases with a neovascular component. ..
- Does the extreme variability of methadone metabolism have an epigenetic cause?Gavin R Schnitzler; Fiscal Year: 2010....
- Modulation of Neonatal Hyperoxic Lung Injury by the Aryl Hydrocarbon ReceptorBinoy Shivanna; Fiscal Year: 2013..I anticipate that the results will support my hypotheses, which will lead to novel strategies in the prevention and treatment of BPD in premature infants. ..
- Mechanisms and Functions of Bimodally Targeted Cytochrome P450s to MitochondriaNarayan G Avadhani; Fiscal Year: 2012..Finally, the role of mitochondria targeted CYPs in ischemia reperfusion injury will be studied using cell and animal models. ..
- Chemoprevention of Pancreatic Cancer by CapsaicinSanjay K Srivastava; Fiscal Year: 2013..Successful completion of this project will support the development and clinical application of capsaicin for the chemoprevention of pancreatic cancer. ..
- Blood Pressure -- Determinants &ControllersAllen W Cowley; Fiscal Year: 2012....
- Genomic profiling of yeast resistance to AFB1, a P450-activated carcinogenMICHAEL THOMAS FASULLO; Fiscal Year: 2013..The project will be a valuable training tool in systems and computational biology. ..
- Function of mRNA Editing in TrypanosomesStephen L Hajduk; Fiscal Year: 2010..Together these studies will provide the first analysis of the role of RNA editing in protein diversification in trypanosomes and is likely to lead to the discovery of novel mitochondrial proteins. ..
- Regulation of Drug Metabolizing Enzyme OntogenyD Gail McCarver; Fiscal Year: 2012..This knowledge will be invaluable in modifying risk policies to minimize/avoid adverse drug events and toxicity due to environmental exposures in children. ..
- Mechanisms of Cell Death after Photodynamic TherapyAnna Liisa Nieminen; Fiscal Year: 2010..The proposed studies will enhance our understanding how to enhance PDT-induced killing of cancer cells and further increase the efficacy of PDT translation to the benefit for cancer patients. ..
- Role of NADPH oxidase 1-derived ROS in the pathogenesis of Parkinson's diseaseYoon Seong Kim; Fiscal Year: 2012..abstract_text> ..
- Participation in Cytochrome b5 in Anesthetic MetabolismLucy A Waskell; Fiscal Year: 2010DESCRIPTION (provided by applicant): The cytochromes P450 (cyts P450) are a ubiquitous superfamily of oxidases whose activity is influenced by a second protein, cytochrome b5 (cyt b5)...
- Problems in Membrane Protein Crystallography: Hetero-Oligomeric Cytochrome b6fWilliam A Cramer; Fiscal Year: 2012..Via the membrane, the set of energy-transducing proteins determines the level of energy and its regulation in the human cell. ..
- Role of Mitochondrial Aldehyde Dehydrogenases in Ethanol Metabolism and ToxicityVasilis Vasiliou; Fiscal Year: 2010..This will be accomplished by studying the formation of heterotetramers using recombinant proteins and cell lines expressing these proteins. ..
- Polymorphisms of estrogen-metabolizing genes in kidney cancerYuichiro Tanaka; Fiscal Year: 2013..Such information can lead to the identification of Veterans with higher risk for RCC. Proposed research may also lead to implications for possible therapy for RCC. ..
- REACTION MECHANISM OF MYOCARDIAC UBIQUINONE-PROTEINSChang An Yu; Fiscal Year: 2009..Qo site pocket, the proton transfer path, structure water, hydrogen bonding, and the interaction domain between cytochromes c\ and c...
- CYTOCHROME B5--A CASE STUDY IN MOLECULAR RECOGNITIONMario Rivera; Fiscal Year: 1999b>Cytochromes b are an important class of hemoproteins involved in electron transfer reactions...
- OXIDATIVE PHOSPHORYLATION IN CARDIAC HYPERTROPHYTERRENCE O BRIEN; Fiscal Year: 2000..Studying the genetic regulation of oxidative phosphorylation in cardiocytes subjected to hypertrophic stimulus should lead to important, physiologically relevant, insights into key initial molecular events. ..
- ELECTRON TRANSPORT ENZYMES IN CATECHOLAMINE GRANULESPatrick Fleming; Fiscal Year: 1990..DBH was not previously known to be allosterically regulated. Thus, this characterization is necessary to permit an understanding of the regulation of catecholamine biosynthesis...
- Cardiolipin Function in Mitochondrial Electron-Transfer ComplexesNeal C Robinson; Fiscal Year: 2011..Successful completion of this project will determine the absolute requirement for cardiolipin and help us understand its pathophysiological importance. ..
- Prostaglandin 19-& 20-Hydroxylation by Cytochrome P450Bettie Sue Masters; Fiscal Year: 2005..on the determination of molecular and kinetic properties of four members of the CYP4A gene subfamily of rabbit cytochromes P450 that hydroxylate medium and/or long chain fatty acids and eIcosanoids primarily or exclusively in the ..
- Cytochrome-P-450 genes in pathobiology of endometrial cancerRajvir Dahiya; Fiscal Year: 2009..This project will provide us with novel mechanisms involving the pathobiology of endometrial cancer. ..
- ELECTRON-TRANSFER PROTEINSFRANCIS S contact MILLETT; Fiscal Year: 2010..In the proposed studies, new ruthenium photoexcitation methods will be developed to study electron transfer reactions important to human health. ..
- 3-D CRYSTALLIZATION OF MEMBRANE PROTEINSJAMES GOUAUX; Fiscal Year: 1999..Extending the methods elucidated from this research to other systems will provide a structural understanding of such human conditions as cystic fibrosis, retinitis pigmentosa and multidrug resistance. ..