pyridoxal phosphate

Summary

Summary: This is the active form of VITAMIN B 6 serving as a coenzyme for synthesis of amino acids, neurotransmitters (serotonin, norepinephrine), sphingolipids, aminolevulinic acid. During transamination of amino acids, pyridoxal phosphate is transiently converted into pyridoxamine phosphate (PYRIDOXAMINE).

Top Publications

  1. ncbi Pyridoxal 5'-phosphate enzymes as targets for therapeutic agents
    Alessio Amadasi
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Parma, Italy
    Curr Med Chem 14:1291-324. 2007
  2. ncbi Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerations
    Andrew C Eliot
    Department of Chemistry University of California, Berkeley, California 94720 3206, USA
    Annu Rev Biochem 73:383-415. 2004
  3. pmc Association of vitamin B-6 status with inflammation, oxidative stress, and chronic inflammatory conditions: the Boston Puerto Rican Health Study
    Jian Shen
    Jean Mayer US Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, 711 Washington Street, Boston, MA 02111 1524, USA
    Am J Clin Nutr 91:337-42. 2010
  4. ncbi Consequences of missense mutations for dimerization and turnover of alanine:glyoxylate aminotransferase: study of a spectrum of mutations
    M B Coulter-Mackie
    Department of Pediatrics, University of British Columbia, Children s and Women s Health Centre, Vancouver, BC, Canada
    Mol Genet Metab 89:349-59. 2006
  5. ncbi Human liver peroxisomal alanine:glyoxylate aminotransferase: Different stability under chemical stress of the major allele, the minor allele, and its pathogenic G170R variant
    Barbara Cellini
    Dipartimento di Scienze della Vita e della Riproduzione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8 37134 Verona, Italy
    Biochimie 92:1801-11. 2010
  6. ncbi Crystal structure of alanine:glyoxylate aminotransferase and the relationship between genotype and enzymatic phenotype in primary hyperoxaluria type 1
    Xiaoxuan Zhang
    Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    J Mol Biol 331:643-52. 2003
  7. pmc Crystal structure of glutamate-1-semialdehyde aminomutase: an alpha2-dimeric vitamin B6-dependent enzyme with asymmetry in structure and active site reactivity
    M Hennig
    Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    Proc Natl Acad Sci U S A 94:4866-71. 1997
  8. pmc The mechanism of addition of pyridoxal 5'-phosphate to Escherichia coli apo-serine hydroxymethyltransferase
    Francesca Malerba
    Dipartimento di Scienze Biochimiche A Rossi Fanelli, Universita degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
    Biochem J 404:477-85. 2007
  9. ncbi Plasma pyridoxal 5'-phosphate in the US population: the National Health and Nutrition Examination Survey, 2003-2004
    Martha Savaria Morris
    Jean Mayer US Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA
    Am J Clin Nutr 87:1446-54. 2008
  10. pmc Molecular basis of reduced pyridoxine 5'-phosphate oxidase catalytic activity in neonatal epileptic encephalopathy disorder
    Faik N Musayev
    Department of Medicinal Chemistry, School of Pharmacy, and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia 23219, USA
    J Biol Chem 284:30949-56. 2009

Detail Information

Publications350 found, 100 shown here

  1. ncbi Pyridoxal 5'-phosphate enzymes as targets for therapeutic agents
    Alessio Amadasi
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Parma, Italy
    Curr Med Chem 14:1291-324. 2007
    ..Then, PLP-dependent enzymes that have been recently characterized and proposed as drug targets are reported. Finally, the relevance of recent genomic analysis of PLP-dependent enzymes for the selection of drug targets is discussed...
  2. ncbi Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerations
    Andrew C Eliot
    Department of Chemistry University of California, Berkeley, California 94720 3206, USA
    Annu Rev Biochem 73:383-415. 2004
    b>Pyridoxal phosphate (PLP)-dependent enzymes are unrivaled in the diversity of reactions that they catalyze...
  3. pmc Association of vitamin B-6 status with inflammation, oxidative stress, and chronic inflammatory conditions: the Boston Puerto Rican Health Study
    Jian Shen
    Jean Mayer US Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, 711 Washington Street, Boston, MA 02111 1524, USA
    Am J Clin Nutr 91:337-42. 2010
    ..Low vitamin B-6 status has been linked to an increased risk of cardiovascular diseases. The cardioprotective effects of vitamin B-6 independent of homocysteine suggest that additional mechanisms may be involved...
  4. ncbi Consequences of missense mutations for dimerization and turnover of alanine:glyoxylate aminotransferase: study of a spectrum of mutations
    M B Coulter-Mackie
    Department of Pediatrics, University of British Columbia, Children s and Women s Health Centre, Vancouver, BC, Canada
    Mol Genet Metab 89:349-59. 2006
    ..The effect of pyridoxal phosphate (PLP) on dimerization and stability was also investigated...
  5. ncbi Human liver peroxisomal alanine:glyoxylate aminotransferase: Different stability under chemical stress of the major allele, the minor allele, and its pathogenic G170R variant
    Barbara Cellini
    Dipartimento di Scienze della Vita e della Riproduzione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8 37134 Verona, Italy
    Biochimie 92:1801-11. 2010
    ..1 M urea. These biochemical results are discussed in the light of the characteristics of the enzymatic phenotype of PH1 patients bearing G170R mutation in AGT-Mi and the positive response of these patients to pyridoxine treatment...
  6. ncbi Crystal structure of alanine:glyoxylate aminotransferase and the relationship between genotype and enzymatic phenotype in primary hyperoxaluria type 1
    Xiaoxuan Zhang
    Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    J Mol Biol 331:643-52. 2003
    ..such as accelerated proteolysis (Ser205Pro), intra-peroxisomal aggregation (Gly41Arg), inhibition of pyridoxal phosphate binding and loss of catalytic activity (Gly82Glu), and peroxisome-to-mitochondrion mistargeting (Gly170Arg)...
  7. pmc Crystal structure of glutamate-1-semialdehyde aminomutase: an alpha2-dimeric vitamin B6-dependent enzyme with asymmetry in structure and active site reactivity
    M Hennig
    Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    Proc Natl Acad Sci U S A 94:4866-71. 1997
    ..In the unliganded enzyme, one subunit has the cofactor bound as an aldimine of pyridoxal phosphate with Lys-273 and, in this subunit, residues 153-181 are disordered...
  8. pmc The mechanism of addition of pyridoxal 5'-phosphate to Escherichia coli apo-serine hydroxymethyltransferase
    Francesca Malerba
    Dipartimento di Scienze Biochimiche A Rossi Fanelli, Universita degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
    Biochem J 404:477-85. 2007
    ....
  9. ncbi Plasma pyridoxal 5'-phosphate in the US population: the National Health and Nutrition Examination Survey, 2003-2004
    Martha Savaria Morris
    Jean Mayer US Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA
    Am J Clin Nutr 87:1446-54. 2008
    ....
  10. pmc Molecular basis of reduced pyridoxine 5'-phosphate oxidase catalytic activity in neonatal epileptic encephalopathy disorder
    Faik N Musayev
    Department of Medicinal Chemistry, School of Pharmacy, and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia 23219, USA
    J Biol Chem 284:30949-56. 2009
    ..These results provide a molecular basis for the phenotype associated with the R229W mutation, as well as providing a foundation for understanding the pathophysiological consequences of pyridoxine 5'-phosphate oxidase mutations...
  11. ncbi Overexpression of human alanine:glyoxylate aminotransferase in Escherichia coli: renaturation from guanidine-HCl and affinity for pyridoxal phosphate co-factor
    Marion B Coulter-Mackie
    Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada V6H 3N1
    Protein Expr Purif 41:18-26. 2005
    ..AGT requires a pyridoxal phosphate (PLP) co-factor in its active site...
  12. ncbi The human pyridoxal kinase, a plausible target for ginkgotoxin from Ginkgo biloba
    Uta Kästner
    Institut fur Pharmazeutische Biologie, Universitat Bonn, Germany
    FEBS J 274:1036-45. 2007
    ..Thus, the presence of ginkgotoxin leads to temporarily reduced pyridoxal phosphate formation in vitro and possibly also in vivo...
  13. ncbi Update on interconversions of vitamin B-6 with its coenzyme
    D B McCormick
    Department of Biochemistry, Rollins Research Center, Emory University, Atlanta, GA 30322 3050, USA
    J Nutr 129:325-7. 1999
    ..Both kinase and oxidase involved in B-6 metabolism are potential targets for pharmacologic agents...
  14. pmc Purinergic receptor activation evokes neurotrophic factor neuropeptide Y release from neonatal mouse olfactory epithelial slices
    Shami Kanekar
    Department of Physiology, University of Utah, Salt Lake City, Utah, USA
    J Neurosci Res 87:1424-34. 2009
    ....
  15. ncbi Improvement of the therapeutic index of anticancer drugs by the superoxide dismutase mimic mangafodipir
    Jerome Alexandre
    université Paris René Descartes, Faculte de Medecine, Assistance Publique Hopitaux de Paris, Hopital Cochin, EA 1833, Paris, France
    J Natl Cancer Inst 98:236-44. 2006
    ..We investigated its and other oxidative stress modulators' effects on anticancer drug activity in vitro and in vivo...
  16. ncbi Redox regulation and reaction mechanism of human cystathionine-beta-synthase: a PLP-dependent hemesensor protein
    Ruma Banerjee
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Arch Biochem Biophys 433:144-56. 2005
    ..of the hydroxyl group of serine with the thiolate of homocysteine and is unique in being the only known pyridoxal phosphate-dependent enzyme that also contains heme b as a cofactor...
  17. ncbi The relationship between plasma and red cell concentrations of vitamins thiamine diphosphate, flavin adenine dinucleotide and pyridoxal 5-phosphate following elective knee arthroplasty
    Andrew Gray
    Department of Orthopaedics, Royal Infirmary, Glasgow G31 2ER, UK
    Clin Nutr 23:1080-3. 2004
    ..The aim of the study was to examine the relationship between plasma and intra-cellular B-vitamins following the systemic inflammatory response of surgery...
  18. ncbi Conversion of a PLP-dependent racemase into an aldolase by a single active site mutation
    Florian P Seebeck
    Laboratorium fur Organische Chemie, Swiss Federal Institute of Technology, ETH Honggerberg, CH 8093 Zurich, Switzerland
    J Am Chem Soc 125:10158-9. 2003
    ..This preference is consistent with the expected binding mode of substrate in the modified active site and supports the proposal that naturally occurring d-threonine aldolases and alanine racemases derive from a common ancestor...
  19. ncbi Nucleotide-induced Ca2+ signaling in sustentacular supporting cells of the olfactory epithelium
    Thomas Hassenklöver
    Department of Neurophysiology and Cellular Biophysics, University of Gottingen, Humboldtallee 23, Gottingen, Germany
    Glia 56:1614-24. 2008
    ..Our findings suggest that extracellular nucleotides in the OE activate SCs via P2Y2/P2Y4-like receptors and initiate a characteristic intraepithelial Ca2+ wave...
  20. ncbi Low plasma vitamin B-6 status affects metabolism through the kynurenine pathway in cardiovascular patients with systemic inflammation
    Oivind Midttun
    Bevital A S, Laboratoriebygget, 5021 Bergen, Norway
    J Nutr 141:611-7. 2011
    ..Thus, low plasma PLP during inflammation may reflect impaired cellular vitamin B-6 status, as indicated by the concurrent increase in plasma HK...
  21. ncbi Relation between pyridoxal and pyridoxal phosphate concentrations in plasma, red cells, and white cells in patients with critical illness
    Aikaterini T Vasilaki
    University Department of Surgery, Royal Infirmary, Glasgow, United Kingdom
    Am J Clin Nutr 88:140-6. 2008
    ..Evidence suggests that the relation between plasma and red cell vitamin B-6 concentrations is perturbed as part of the systemic inflammatory response in critically ill patients...
  22. ncbi Crystal structure of rabbit cytosolic serine hydroxymethyltransferase at 2.8 A resolution: mechanistic implications
    J N Scarsdale
    Institute of Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond 23219, USA
    Biochemistry 38:8347-58. 1999
    ..SHMT requires both pyridoxal phosphate (PLP) and tetrahydropteroylpolyglutamate (H4PteGlun) as cofactors, the latter as a carrier of the single ..
  23. pmc The tryptophan synthase α2β2 complex: a model for substrate channeling, allosteric communication, and pyridoxal phosphate catalysis
    Edith Wilson Miles
    Laboratory of Biochemistry and Genetics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 288:10084-91. 2013
    I reflect on my research on pyridoxal phosphate (PLP) enzymes over fifty-five years and on how I combined research with marriage and family. My Ph.D. research with Esmond E. Snell established one aspect of PLP enzyme mechanism...
  24. pmc NPY mediates ATP-induced neuroproliferation in adult mouse olfactory epithelium
    Cuihong Jia
    Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, 48824, USA
    Neurobiol Dis 38:405-13. 2010
    ..These data indicate that ATP initiates neuroproliferation via NPY upregulation, NPY release, and Y1 receptor activation, and suggests that the olfactory epithelium is good model to study neuroregenerative mechanisms in the CNS...
  25. pmc Association of blood lead (Pb) and plasma homocysteine: a cross sectional survey in Karachi, Pakistan
    Mohsin Yakub
    Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan
    PLoS ONE 5:e11706. 2010
    ..The objective of the present study was to assess the relationship of blood Pb to the risk of hyperhomocysteinemia in a low income urban population of Karachi, Pakistan...
  26. ncbi Pyridoxal phosphate-dependent neonatal epileptic encephalopathy
    S Bagci
    Centre for Paediatrics, Department of Neonatology, Sigmund Freud Strasse 25, 53105 Bonn, Germany
    Arch Dis Child Fetal Neonatal Ed 93:F151-2. 2008
    Pyridox(am)ine-5'-phosphate oxidase converts pyridoxine phosphate and pyridoxamine phosphate to pyridoxal phosphate, a cofactor in many metabolic reactions, including neurotransmitter synthesis...
  27. ncbi Functional synergism between the most common polymorphism in human alanine:glyoxylate aminotransferase and four of the most common disease-causing mutations
    M J Lumb
    Medical Research Council Laboratory for Molecular Cell Biology and the Department of Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 275:36415-22. 2000
    ..These results are discussed in the light of the high allelic frequency ( approximately 20%) of the P11L polymorphism and its importance in determining the phenotypic manifestations of mutations in PH1...
  28. ncbi Spectroscopic and kinetic analyses reveal the pyridoxal 5'-phosphate binding mode and the catalytic features of Treponema denticola cystalysin
    Mariarita Bertoldi
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    Biochemistry 41:9153-64. 2002
    ..In addition, reinvestigation of the substrate spectrum of alpha,beta-elimination indicates that cystalysin is a cyst(e)ine C-S lyase rather than a cysteine desulfhydrase as claimed previously...
  29. pmc Conserved YjgF protein family deaminates reactive enamine/imine intermediates of pyridoxal 5'-phosphate (PLP)-dependent enzyme reactions
    Jennifer A Lambrecht
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 287:3454-61. 2012
    ..The conservation of the RidA/YjgF family suggests that reactive enamine/imine metabolites are of concern to all organisms...
  30. ncbi l-Threonine aldolase, serine hydroxymethyltransferase and fungal alanine racemase. A subgroup of strictly related enzymes specialized for different functions
    R Contestabile
    Dipartimento di Scienze Biochimiche A Rossi Fanelli and Centro di Biologia Molecolare del Consiglio Nazionale delle Ricerche, Universita degli Studi di Roma, La Sapienza, Roma, Italy
    Eur J Biochem 268:6508-25. 2001
    ..coli and alanine racemase from Cochliobolus carbonum, and their comparison with the SHMT crystal structure, indicated how the tetrahydrofolate binding site might have evolved and offered a starting point for further investigations...
  31. ncbi Pyridoxal phosphate decreases in plasma but not erythrocytes during systemic inflammatory response
    Dinesh Talwar
    Department of Biochemistry, Royal Infirmary, Glasgow G31 2ER, United Kingdom
    Clin Chem 49:515-8. 2003
  32. pmc In vivo and in vitro examination of stability of primary hyperoxaluria-associated human alanine:glyoxylate aminotransferase
    Erin D Hopper
    Department of Biology and Chemistry, Duke University, Durham, North Carolina 27708, USA
    J Biol Chem 283:30493-502. 2008
    ..e. pharmacological chaperones) may be effective for treatment of primary hyperoxaluria, we propose that the methods described here can be used in high throughput screens for compounds that stabilize AGT mutants...
  33. ncbi Phylogenetic analyses and comparative genomics of vitamin B6 (pyridoxine) and pyridoxal phosphate biosynthesis pathways
    G Mittenhuber
    Institut fur Mikrobiologie und Molekularbiologie, Ernst Moritz Arndt Universitat, Greifswald, Germany
    J Mol Microbiol Biotechnol 3:1-20. 2001
    Vitamin B6 in its active form pyridoxal phosphate is an essential coenzyme of many diverse enzymes. Biochemistry, enzymology and genetics of de novo vitamin B6 biosynthesis have been primarily investigated in Escherichia coli...
  34. pmc Biochemical analyses are instrumental in identifying the impact of mutations on holo and/or apo-forms and on the region(s) of alanine:glyoxylate aminotransferase variants associated with primary hyperoxaluria type I
    Elisa Oppici
    Department of Life Sciences and Reproduction, Section of Biological Chemistry, University of Verona, Strada Le Grazie 8 37134 Verona, Italy
    Mol Genet Metab 105:132-40. 2012
    ..e. active site microenvironment, large and/or small domain) of the protein are affected by each mutation, and (iii) suggest the possible therapeutic approach for patients bearing the examined mutations...
  35. ncbi D-Cysteine desulfhydrase of Escherichia coli. Purification and characterization
    T Nagasawa
    Eur J Biochem 153:541-51. 1985
    ..5-10.5), and contains 2 mol pyridoxal phosphate/mol enzyme...
  36. pmc Structure of a bacterial pyridoxal 5'-phosphate synthase complex
    Marco Strohmeier
    Heidelberg University Biochemistry Center, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 103:19284-9. 2006
    ..Mutagenesis permitted identification of the remote glutaminase and synthase catalytic centers and led us to propose a mechanism whereby ammonia shuttles between these active sites through a methionine-rich hydrophobic tunnel...
  37. ncbi A new fatal case of pyridox(am)ine 5'-phosphate oxidase (PNPO) deficiency
    Angeles Ruiz
    Department of Pediatrics, Hospital Son Dureta, Palma de Mallorca, Spain
    Mol Genet Metab 93:216-8. 2008
    ..A174X, in the PNPO gene who died with pyridoxal phosphate (PLP) treatment despite initial clinical recovery...
  38. pmc Vitamin B-6 intake is inversely related to, and the requirement is affected by, inflammation status
    Martha Savaria Morris
    Nutritional Epidemiology Program, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA
    J Nutr 140:103-10. 2010
    ....
  39. ncbi The multifaceted pyridoxal 5'-phosphate-dependent O-acetylserine sulfhydrylase
    Andrea Mozzarelli
    Department of Biochemistry and Molecular Biology, University of Parma, Parma, Italy
    Biochim Biophys Acta 1814:1497-510. 2011
    ..Given the key biological role played by O-acetylserine sulfhydrylase in bacteria, inhibitors with potential antibiotic activity have been developed. This article is part of a Special Issue entitled: Pyridoxal Phospate Enzymology...
  40. ncbi B6-responsive disorders: a model of vitamin dependency
    Peter T Clayton
    Biochemistry, Endocrinology and Metabolism, Institute of Child Health, 30 Guilford St, London, WC1N 1 EH, UK
    J Inherit Metab Dis 29:317-26. 2006
    b>Pyridoxal phosphate is the cofactor for over 100 enzyme-catalysed reactions in the body, including many involved in the synthesis or catabolism of neurotransmitters...
  41. ncbi Haemolysis induced by α-toxin from Staphylococcus aureus requires P2X receptor activation
    Marianne Skals
    Department of Biomedicine, Aarhus University, Ole Worms Alle 4, build 1160, 8000, Aarhus C, Denmark
    Pflugers Arch 462:669-79. 2011
    ..coli produces cell damage by specific activation of a purinergic signalling cascade. These data indicate that pore-forming toxins in general require purinergic signalling to elicit their toxicity...
  42. ncbi Treponema denticola cystalysin catalyzes beta-desulfination of L-cysteine sulfinic acid and beta-decarboxylation of L-aspartate and oxalacetate
    Barbara Cellini
    Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8, 37134 Verona, Italy
    FEBS Lett 554:306-10. 2003
    ..The k(cat) and K(m) of this reaction are 0.15+/-0.01 s(-1) and 13+/-2 mM, respectively. Possible mechanistic and physiological implications are discussed...
  43. ncbi X-ray structure of Escherichia coli pyridoxine 5'-phosphate oxidase complexed with pyridoxal 5'-phosphate at 2.0 A resolution
    M K Safo
    Institute for Structural Biology and Drug Discovery, and Department of Biochemistry, Virginia Commonwealth University, Richmond, VA 23219, USA
    J Mol Biol 310:817-26. 2001
    ..A possible tunnel exists between the two sites so that pyridoxal 5'-phosphate formed at the active site may transfer to the non-catalytic site without passing though the solvent...
  44. ncbi Ginkgo seed poisoning
    Yo Kajiyama
    Department of Pediatrics Kyoto Min i ren Central Hospital Kyoto, Japan
    Pediatrics 109:325-7. 2002
    ..usually involve children who exhibit repetitive seizures that can be fatal, prompt administration of pyridoxal phosphate (2 mg/kg) may have prevented additional seizures...
  45. ncbi Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly
    J T Kaiser
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, Martinsried, D 82152, Germany
    J Mol Biol 297:451-64. 2000
    ....
  46. ncbi NMR studies of coupled low- and high-barrier hydrogen bonds in pyridoxal-5'-phosphate model systems in polar solution
    Shasad Sharif
    Institut fur Chemie und Biochemie, Takustrasse 3, Freie Universitat Berlin, D 14195 Berlin, Germany
    J Am Chem Soc 129:6313-27. 2007
    ..Therefore, the reactivity of Schiff base intermediates in pyridoxal-5'-phosphate enzymes can likely be tuned to the requirements of the reaction being catalyzed by differential protonation of the pyridine nitrogen...
  47. ncbi Structure, mechanism, and conformational dynamics of O-acetylserine sulfhydrylase from Salmonella typhimurium: comparison of A and B isozymes
    Arundhati Chattopadhyay
    Institute of Materials Science, University of Connecticut, 97 North Eagleville Road, Storrs, Connecticut 06269 3136, USA
    Biochemistry 46:8315-30. 2007
    ..This finding, in agreement with structural data, suggests for the O-acetylserine sulfhydrylase B-isozyme a higher degree of conformational flexibility linked to catalysis...
  48. pmc Activation of purinergic receptor subtypes modulates odor sensitivity
    Colleen C Hegg
    Department of Physiology, University of Utah, Salt Lake City, Utah 84108 1297, USA
    J Neurosci 23:8291-301. 2003
    ..Peripheral ATP-mediated odor suppression is a novel mechanism for reduced olfactory sensitivity during exposure to olfactotoxins and may be a novel neuroprotective mechanism...
  49. ncbi Histidine decarboxylase, a pyridoxal phosphate-dependent enzyme, is an autoantigen of gastric enterochromaffin-like cells
    Filip Sköldberg
    Department of Medical Sciences, Uppsala University, University Hospital, 751 85 Uppsala, Sweden
    J Clin Endocrinol Metab 88:1445-52. 2003
    ..syndrome type 1 often have autoantibodies against neurotransmitter synthesizing enzymes, including the pyridoxal phosphate-dependent enzymes glutamic acid decarboxylase and aromatic L-amino acid decarboxylase...
  50. pmc Electrical stimulation of the mucosa evokes slow EPSPs mediated by NK1 tachykinin receptors and by P2Y1 purinoceptors in different myenteric neurons
    Rachel M Gwynne
    Department of Physiology, University of Melbourne, Parkville, Victoria 3010, Australia
    Am J Physiol Gastrointest Liver Physiol 297:G179-86. 2009
    ..3-9 s) EPSPs. The slow EPSPs were blocked by pyridoxal phosphate-6-axophenyl-2-4-disulfonic acid (30 microM, N = 3) or the specific P2Y(1) antagonist MRS 2179 (10 microM, N ..
  51. ncbi Plasma pyridoxal phosphate and pyridoxic acid and their relationship to plasma homocysteine in a representative sample of British men and women aged 65 years and over
    C J Bates
    MRC Human Nutrition Research, Cambridge, UK
    Br J Nutr 81:191-201. 1999
    Concentrations of pyridoxal phosphate and pyridoxic acid were measured in fasting plasma samples from British men and women aged 65 years and over, participating in a National Diet and Nutrition Survey during 1994-5, selected to be ..
  52. ncbi D-amino acids in the brain: structure and function of pyridoxal phosphate-dependent amino acid racemases
    Tohru Yoshimura
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, Japan
    FEBS J 275:3527-37. 2008
    ..We compared the eukaryotic serine racemase with bacterial alanine racemase, the best-studied enzyme among the PLP-dependent amino acid racemases, and thus suggested a putative reaction mechanism for mammalian D-serine synthesis...
  53. ncbi Structure and control of pyridoxal phosphate dependent allosteric threonine deaminase
    D T Gallagher
    University of Maryland, Biotechnology Institute, National Institute of Standards and Technology 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Structure 6:465-75. 1998
    ..This enzyme is thus a useful model system for studying the structural basis of allosteric control mechanisms...
  54. ncbi Tryptophan synthase: a mine for enzymologists
    Samanta Raboni
    Department of Biochemistry and Molecular Biology, University of Parma, Viale G P Usberti 23 A, Parma, Italy
    Cell Mol Life Sci 66:2391-403. 2009
    ..These key features have made tryptophan synthase a text-book case for the understanding of the interplay between chemistry and conformational energy landscapes...
  55. ncbi P2 receptor-mediated stimulation of the PI3-K/Akt-pathway in vivo
    H Franke
    Rudolf Boehm Institute of Pharmacology and Toxicology, University of Leipzig, Leipzig, Germany
    Glia 57:1031-45. 2009
    ....
  56. ncbi Bacterial cysteine desulfurases: their function and mechanisms
    H Mihara
    Institute for Chemical Research, Kyoto University, Uji, Japan
    Appl Microbiol Biotechnol 60:12-23. 2002
    ....
  57. ncbi Glycine turnover and decarboxylation rate quantified in healthy men and women using primed, constant infusions of [1,2-(13)C2]glycine and [(2)H3]leucine
    Yvonne Lamers
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    J Nutr 137:2647-52. 2007
    ..GCS is responsible for a substantial proportion of whole body glycine flux and constitutes a major route for the generation of 1-carbon units...
  58. ncbi On the two components of pyridoxal 5'-phosphate synthase from Bacillus subtilis
    Thomas Raschle
    ETH Zurich, Institut für Pflanzenwissenschaften, Zurich, Switzerland
    J Biol Chem 280:32291-300. 2005
    ..The results are discussed in light of the nature of the interaction of the two components of the enzyme complex...
  59. ncbi Escherichia coli contains a protein that is homologous in function and N-terminal sequence to the protein encoded by the nifS gene of Azotobacter vinelandii and that can participate in the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase
    D H Flint
    E I Du Pont de Nemours and Company, Central Research and Development, Experimental Station, Wilmington, Delaware 19880 0328, USA
    J Biol Chem 271:16068-74. 1996
    ..This E. coli protein contains pyridoxal phosphate as a cofactor and catalyzes the removal of sulfur from cysteine to form alanine and S0...
  60. ncbi Structure of external aldimine of Escherichia coli CsdB, an IscS/NifS homolog: implications for its specificity toward selenocysteine
    Hisaaki Mihara
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biochem 131:679-85. 2002
    ..These facts suggest that the restricted flexibility of the Cys364-anchoring extended lobe in CsdB may be responsible for the ability of the enzyme to discriminate between selenium and sulfur...
  61. pmc Structure of human cystathionine beta-synthase: a unique pyridoxal 5'-phosphate-dependent heme protein
    M Meier
    M E Müller Institute for Structural Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    EMBO J 20:3910-6. 2001
    ..This heme binding motif together with a spatially adjacent oxidoreductase active site motif could explain the regulation of its enzyme activity by redox changes...
  62. pmc Poisoning pyridoxal 5-phosphate-dependent enzymes: a new strategy to target the malaria parasite Plasmodium falciparum
    Ingrid B Müller
    Department of Biochemistry, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    PLoS ONE 4:e4406. 2009
    ....
  63. pmc Quantal release of ATP in mouse cortex
    Yuriy Pankratov
    Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK
    J Gen Physiol 129:257-65. 2007
    ..Furthermore, the packets of ATP appear to be released from vesicle in a subset of glutamate-containing terminals...
  64. pmc Identification of regions of the P2X(7) receptor that contribute to human and rat species differences in antagonist effects
    A D Michel
    Neurology Centre of Excellence for Drug Discovery, GlaxoSmithKline Research and Development Limited, New Frontiers Science Park, Harlow, Essex, UK
    Br J Pharmacol 155:738-51. 2008
    ..In this study we have identified several regions of the P2X(7) receptor that contribute to the species differences in antagonist effects...
  65. ncbi P2X7 receptor inhibition improves recovery after spinal cord injury
    Xiaohai Wang
    Department of Neurosurgery, Center for Aging and Developmental Biology, University of Rochester Medical Center, Rochester, New York 14642, USA
    Nat Med 10:821-7. 2004
    ..P2X7R antagonists inhibit this process, reducing both the histological extent and functional sequelae of acute SCI...
  66. ncbi Reconstitution and biochemical characterization of a new pyridoxal-5'-phosphate biosynthetic pathway
    Kristin E Burns
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA
    J Am Chem Soc 127:3682-3. 2005
    ..The substrates for Bacillus subtilis PLP synthase (YaaD and YaaE) are identified, and the first reconstitution of PLP biosynthesis using this pathway is described. Three partial reactions catalyzed by YaaD are also identified...
  67. ncbi Structure and mechanism of Escherichia coli pyridoxine 5'-phosphate oxidase
    Martino L di Salvo
    Dipartimento di Scienze Biochimiche A Rossi Fanelli, Universita La Sapienza, P le Aldo Moro 5, 00185, Via degli Apuli 9, Rome, Italy
    Biochim Biophys Acta 1647:76-82. 2003
    ..The crystal structure and the stereospecificity studies suggest that the pair of electrons on C4' of the substrate are transferred to FMN as a hydride ion...
  68. ncbi Exploring the pyridoxal 5'-phosphate-dependent enzymes
    Andrea Mozzarelli
    Department of Biochemistry and Molecular Biology, University of Parma, 43100 Parma, Italy
    Chem Rec 6:275-87. 2006
    ..Finally, perspectives of PLP-dependent enzymes functional genomics and drug targeting highlight the continuous novelty of an "old" class of enzymes...
  69. ncbi Mangafodipir prevents liver injury induced by acetaminophen in the mouse
    Sassia Bedda
    Laboratoire d Immunologie, UPRES 1833, Faculte Cochin, Pavillon Hardy, Universite Paris V, AP HP, 75679 Paris Cedex 14, France
    J Hepatol 39:765-72. 2003
    ....
  70. ncbi Reaction specificity in pyridoxal phosphate enzymes
    Michael D Toney
    Department of Chemistry, University of California Davis, One Shields Ave, Davis, CA 95616, USA
    Arch Biochem Biophys 433:279-87. 2005
    b>Pyridoxal phosphate enzymes catalyze a wide variety of reaction types on amines and amino acids, generally by stabilizing carbanionic intermediates...
  71. ncbi Hepatobiliary contrast agents for contrast-enhanced MRI of the liver: properties, clinical development and applications
    Peter Reimer
    Department of Radiology, Stadtisches Klinikum Karlsruhe, Academic Teaching Hospital of the University of Freiburg, Moltkestrasse 90, 76133 Karlsruhe, Germany
    Eur Radiol 14:559-78. 2004
    ..No comparative studies have been conducted comparing the advantages or disadvantages of all three agents directly against each other...
  72. ncbi Genomic distribution and heterogeneity of MocR-like transcriptional factors containing a domain belonging to the superfamily of the pyridoxal-5'-phosphate dependent enzymes of fold type I
    E Bramucci
    Dipartimento di Scienze Biochimiche A Rossi Fanelli, Universita di Roma La Sapienza, 00185 Roma, Italy
    Biochem Biophys Res Commun 415:88-93. 2011
    ..In the vast majority of cases, residues are conserved or conservatively replaced. This result suggests that, in most cases, MocR factors preserve at least ability to bind the cofactor and very likely some catalytic abilities...
  73. ncbi Low vitamin B6 but not homocyst(e)ine is associated with increased risk of stroke and transient ischemic attack in the era of folic acid grain fortification
    Peter J Kelly
    Stroke Service, Department of Neurology, VBK 802, Massachusetts General Hospital, Fruit St, Boston, MA 02114, USA
    Stroke 34:e51-4. 2003
    ..We performed a case-control study to determine the risk of stroke and transient ischemic attack (TIA) associated with tHcy and low vitamin status in a postfortification US sample...
  74. ncbi Pyridoxal 5'-phosphate may be curative in early-onset epileptic encephalopathy
    G F Hoffmann
    Department of General Pediatrics, Ruprecht Karls University Heidelberg, Heidelberg, Germany
    J Inherit Metab Dis 30:96-9. 2007
    ..Prompt treatment with PLP in all neonates and infants with epileptic encephalopathy should become mandatory, permitting normal development in at least some of those affected with PNPO deficiency...
  75. pmc Kinetic properties of polymorphic variants and pathogenic mutants in human cystathionine gamma-lyase
    Weidong Zhu
    Redox Biology Center and Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 47:6226-32. 2008
    ..These results reveal that both mutations weaken the affinity for PLP and suggest that cystathionuric patients with these mutations should be responsive to pyridoxine therapy...
  76. pmc Nickel sulfate induces location-dependent atrophy of mouse olfactory epithelium: protective and proliferative role of purinergic receptor activation
    Cuihong Jia
    Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan 48824, USA
    Toxicol Sci 115:547-56. 2010
    ..These data provide therapeutic strategies to alleviate or cure the loss of olfactory function associated with exposure to nickel compounds...
  77. ncbi X-ray structure refinement and comparison of three forms of mitochondrial aspartate aminotransferase
    C A McPhalen
    Department of Structural Biology, University of Basel, Switzerland
    J Mol Biol 225:495-517. 1992
    ..These adjustments may be accommodated by small rigid-body shifts of secondary structural elements, and by packing defects in the protein core...
  78. ncbi Pyridoxal phosphate binding sites are similar in human heme-dependent and yeast heme-independent cystathionine beta-synthases. Evidence from 31P NMR and pulsed EPR spectroscopy that heme and PLP cofactors are not proximal in the human enzyme
    O Kabil
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664, USA
    J Biol Chem 276:19350-5. 2001
    ..Both classes of enzymes catalyze a pyridoxal phosphate (PLP)-dependent condensation of serine and homocysteine to produce cystathionine...
  79. ncbi Functional attributes of the phosphate group binding cup of pyridoxal phosphate-dependent enzymes
    Alexander I Denesyuk
    Finnish Russian Joint Biotechnology Laboratory, University of Turku, BioCity 6A, Turku, FIN 20520, Finland
    J Mol Biol 316:155-72. 2002
    ..With glucosamine-6-phosphate synthase, the water molecules of the phosphate-binding cup facilitate the entry of substrate and the exit of product...
  80. ncbi Dietary vitamin B-6 restriction does not alter rates of homocysteine remethylation or synthesis in healthy young women and men
    Steven R Davis
    Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611 0370, USA
    Am J Clin Nutr 81:648-55. 2005
    ..The effects of vitamin B-6 status on steady-state kinetics of homocysteine metabolism in humans are unclear...
  81. ncbi Metabolism and pharmacokinetics of MnDPDP in man
    K G Toft
    Nycomed Imaging AS, Oslo, Norway
    Acta Radiol 38:677-89. 1997
    ..To study the metabolism and pharmacokinetics of mangafodipir trisodium injection, 0.01 mmol/ml (Teslascan), in healthy male volunteers...
  82. ncbi Vitamin B6 intakes and status of mechanically ventilated critically ill patients in Taiwan
    Y C Huang
    School of Nutrition, Chung Shan Medical University, Taiwan, Republic of China
    Eur J Clin Nutr 56:387-92. 2002
    ..To assess vitamin B6 intake and status of critically ill patients. The relationship between vitamin B6 status indicators and the severity of illness and outcome in these patients was also examined...
  83. ncbi Metal ion inhibition of nonenzymatic pyridoxal phosphate catalyzed decarboxylation and transamination
    R F Zabinski
    Department of Chemistry, University of California-Davis, One Shields Avenue, Davis, California 95616, USA
    J Am Chem Soc 123:193-8. 2001
    Nonenzymatic pyridoxal phosphate (PLP) catalyzed decarboxylations and transaminations have been revisited experimentally. Metal ions are known to catalyze a variety of PLP-dependent reactions in solution, including transamination...
  84. ncbi Pyridoxal phosphate-responsive seizures in a patient with cerebral folate deficiency (CFD) and congenital deafness with labyrinthine aplasia, microtia and microdontia (LAMM)
    Patricia Dill
    Division of Pediatric Neurology and Developmental Medicine, University Children s Hospital, Basel, Switzerland
    Mol Genet Metab 104:362-8. 2011
    ....
  85. ncbi Role of Asp222 in the catalytic mechanism of Escherichia coli aspartate aminotransferase: the amino acid residue which enhances the function of the enzyme-bound coenzyme pyridoxal 5'-phosphate
    T Yano
    Department of Medical Chemistry, Osaka Medical College, Japan
    Biochemistry 31:5878-87. 1992
    ....
  86. ncbi Properties and interaction of heterologously expressed glutamate decarboxylase isoenzymes GAD(65kDa) and GAD(67kDa) from human brain with ginkgotoxin and its 5'-phosphate
    K Buss
    , , Nussallee 6, 53115 Bonn, Germany
    J Med Chem 44:3166-74. 2001
    ..Comparison of kinetic data, pH, and temperature optima as well as of the mode of interaction with pyridoxal phosphate as a cofactor revealed several significant differences between the two isoenzymes reflecting their somewhat ..
  87. ncbi Intrahepatic biliary anatomy of living adult liver donors: correlation of mangafodipir trisodium-enhanced MR cholangiography and intraoperative cholangiography
    Vibhu Kapoor
    Department of Radiology, Division of Abdominal Imaging, University of Pittsburgh Medical Center, 200 Lothrop St, Pittsburgh, PA 15213, USA
    AJR Am J Roentgenol 179:1281-6. 2002
    ....
  88. ncbi Influence of antivitamins ginkgotoxin 5'-phosphate and deoxypyridoxine 5'-phosphate on human pyridoxine 5'-phosphate oxidase
    Nora Salamon
    Institute of Pharmaceutical Biology, University of Bonn, 53115 Bonn, Germany
    Planta Med 75:563-7. 2009
    ..COLI and the homogeneous enzyme was characterized. The investigation showed that the enzyme is inhibited in vitro by the synthetic vitamin B(6) derivative 4'-deoxypyridoxine 5'-phosphate but not by ginkgotoxin or its 5'-phosphate...
  89. ncbi Ginkgo nut intoxication in a 2-year-old male
    Shunji Hasegawa
    Department of Allergy, Yamaguchi University, School of Medicine, and Department of Hospital Pharmacy, Niigata City General Hospital, Japan
    Pediatr Neurol 35:275-6. 2006
    ..The patient was diagnosed as having ginkgo nut intoxication, and diazepam and pyridoxal phosphate were administered intravenously. After the treatment, his symptoms were resolved...
  90. ncbi Structural analysis of Pseudomonas 1-aminocyclopropane-1-carboxylate deaminase complexes: insight into the mechanism of a unique pyridoxal-5'-phosphate dependent cyclopropane ring-opening reaction
    Subramanian Karthikeyan
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9038, USA
    Biochemistry 43:13328-39. 2004
    ..The results of mutagenesis studies corroborated the assigned critical role for Tyr294 in the catalysis...
  91. ncbi Prevention of 7β-hydroxycholesterol-induced cell death by mangafodipir is mediated through lysosomal and mitochondrial pathways
    Amit Laskar
    Division of Drug Research Pharmacology, Department of Medical and Health Sciences, Faculty of Health Sciences, Linkoping University, SE 581 85 Linkoping, Sweden
    Eur J Pharmacol 640:124-8. 2010
    ..This is the first report to show the additional cytoprotective effect of mangafodipir, which may suggest possible use of the drug...
  92. ncbi Cardiovascular safety of MnDPDP and MnCl2
    P Jynge
    Department of Physiology and Biomedical Engineering, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway
    Acta Radiol 38:740-9. 1997
    ..To investigate the apparent discrepancy between expected basic physiological responses at the cellular level and the in vivo behaviour of both MnDPDP and MnCl2 administered i.v. prompted parallel investigations of these substances...
  93. ncbi Structure of a pseudomerohedrally twinned monoclinic crystal form of a pyridoxal phosphate-dependent catalytic antibody
    Béatrice Golinelli-Pimpaneau
    Laboratoire d Enzymologie et de Biochimie Structurales, Batiment 34, CNRS, 1 Avenue de la Terrasse, 91190 Gif sur Yvette, France
    Acta Crystallogr D Biol Crystallogr 61:472-6. 2005
    ..Four other iodide ions are present in the structure: two are involved in specific intermolecular contacts and two dictate the conformation of the CDRH3 loop in each molecule...
  94. ncbi Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12
    Y Yang
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School 77030 1501, USA
    FEMS Microbiol Lett 141:89-95. 1996
    ....
  95. ncbi Plasma pyridoxal 5'-phosphate is a significant indicator of immune responses in the mechanically ventilated critically ill
    Yi Chia Huang
    School of Nutrition, Chung Shan Medical University, Taichung, Taiwan, Republic of China
    Nutrition 21:779-85. 2005
    ..This study assessed the effect of vitamin B6 status on immune responses in mechanically ventilated, critically ill patients and compared the results with those of healthy controls...
  96. ncbi Reaction mechanism of Escherichia coli cystathionine gamma-synthase: direct evidence for a pyridoxamine derivative of vinylglyoxylate as a key intermediate in pyridoxal phosphate dependent gamma-elimination and gamma-replacement reactions
    P Brzovic
    Department of Biochemistry, University of California, Riverside 92521
    Biochemistry 29:442-51. 1990
    Cystathionine gamma-synthase catalyzes a pyridoxal phosphate dependent synthesis of cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine via a gamma-replacement reaction...
  97. ncbi Bacterial L-serine dehydratases: a new family of enzymes containing iron-sulfur clusters
    R Grabowski
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
    Trends Biochem Sci 18:297-300. 1993
    ..A mechanism for the dehydration of L-serine which is similar, but not identical, to that of the dehydration of citrate catalysed by aconitase is proposed...
  98. ncbi Three-dimensional structure of Escherichia coli branched-chain amino acid aminotransferase at 2.5 A resolution
    K Okada
    Department of Chemistry, Faculty of Science, Osaka City University, Sumiyoshi ku, Osaka
    J Biochem 121:637-41. 1997
    ..Although the main chain conformation of the active site is homologous to that of D-amino acid aminotransferase, many of the active site residues are different between them...
  99. ncbi Crystal structure of serine dehydratase from rat liver
    Taro Yamada
    Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, Kansas 66045 7534, USA
    Biochemistry 42:12854-65. 2003
    ..A possible catalytic mechanism involves the phosphate group, surrounded by the characteristic sequence, acting as a general acid to donate a proton to the leaving hydroxyl group of serine...
  100. ncbi MR imaging properties and pharmacokinetics of MnDPDP in healthy volunteers
    C Wang
    Department of Diagnostic Radiology, University Hospital, Uppsala, Sweden
    Acta Radiol 38:665-76. 1997
    ..Thirteen male volunteers were studied to evaluate the MR imaging properties and pharmacokinetics of 10 mM mangafodipir trisodium infusion (MnDPDP, Teslascan)...
  101. pmc Identification and function of the pdxY gene, which encodes a novel pyridoxal kinase involved in the salvage pathway of pyridoxal 5'-phosphate biosynthesis in Escherichia coli K-12
    Y Yang
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, 77030 1501, USA
    J Bacteriol 180:1814-21. 1998
    ..pdxY is completely cotranscribed with tyrS, but about 92% of tyrS transcripts terminate at a putative Rho-factor-dependent attenuator located in the tyrS-pdxY intercistronic region...

Research Grants62

  1. Cystathionine beta synthase (CBS) and angiogenesis
    Priyabrata Mukherjee; Fiscal Year: 2013
    ....
  2. Vitamin B6 Effects on One-Carbon Metabolism
    Jesse F Gregory; Fiscal Year: 2013
    ..Through its coenzyme form pyridoxal phosphate (PLP), B6 plays essential roles in the acquisition and processing of one-carbon (1C) units by the glycine ..
  3. New Inactivators of GABA Aminotransferase for Addiction
    Richard B Silverman; Fiscal Year: 2013
    ..Studies involving isotopic labeling of 2 and of the pyridoxal phosphate (PLP) coenzyme will be carried out...
  4. Chemically-Rich Structure and Dynamics in the Active Site of Tryptophan Synthase
    Leonard J Mueller; Fiscal Year: 2013
    ....
  5. Elucidation and Evolutionary Potential of a Latent Pathway for PLP Synthesis
    Shelley D Copley; Fiscal Year: 2013
    ..coli has at least three serendipitous pathways that allow synthesis of the essential cofactor pyridoxal phosphate when an enzyme in the normal biosynthetic pathway is missing...
  6. Clinical and Molecular Studies of the Erythropoietic Protoporphyria Phenotype
    Manisha Balwani; Fiscal Year: 2013
    ..pilot study will be conducted in patients with EPP and XLP to determine if Isoniazid, which binds to pyridoxal phosphate, a co-factor of ALAS2, can decrease the activity of ALAS2, thereby reducing the formation of erythrocyte ..
  7. REACTION SPECIFICITY OF PYRIDOXAL PHOSPHATE ENZYMES
    MICHAEL TONEY; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Pyridoxal phosphate (PLP) dependent enzymes are ubiquitous in nitrogen metabolism and catalyze many medically important transformations...
  8. Structure-Function Analysis of AI-2 Quorum Sensing
    Frederick M Hughson; Fiscal Year: 2012
    ..We have purified and crystallized the CAI-1 synthase CqsA, a pyridoxal phosphate enzyme, and in the third aim, we propose to determine its structure, identify its substrates, and ..
  9. Structural Biology of Complex Enzymes
    Janet L Smith; Fiscal Year: 2013
    ..research program on glutamine amidotransferases, focusing on substrate binding and catalytic coupling in pyridoxal phosphate synthase and in CTP synthetase...
  10. MECHANISMS & INHIBITORS OF PYRIDOXAL-DEPENDENT ENZYMES
    DAVID METZLER; Fiscal Year: 1993
    ..coli. The goals will be to provide understanding of basic mechanisms of pyridoxal phosphate- dependent catalysis and to assist in the design of useful enzyme-activated inhibitors...
  11. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
    ..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
  12. INACTIVATION AND STRUCTURE AND GABA AMINOTRANSFERASE
    Richard Silverman; Fiscal Year: 1999
    ....
  13. REGULATION OF ORNITHINE DECARBOXYLASE ACTIVITY
    John Mitchell; Fiscal Year: 1980
    ....
  14. REGULATION OF PYRIDOXAL PHOSPHATE BIOSYNTHESIS IN E COLI
    Malcolm Winkler; Fiscal Year: 1999
    ..Finally, this project is of biomedical and biotechnological relevance, because PLP plays well- documented, diverse roles in human intermediary metabolism, physiology, and disease. ..
  15. VITAMIN B6 ADEQUACY, SMOKING AND DNA DAMAGE
    Terry Shultz; Fiscal Year: 2002
    ..Vitamin B-6 metabolite concentrations [i.e., pyridoxal phosphate (PLP), pyridoxamine phosphate, pyridoxal, pyridoxine and 4-pyridoxic acid] will be determined in plasma and ..
  16. Structure and Mechanisms of PLP Dependent Lyases
    Robert Phillips; Fiscal Year: 2006
    ..The enzymes under study are potential drug targets in pathogens. In addition, both TPL and MGL may be useful in cancer therapy. Thus, knowledge of the structure and mechanism of these enzymes may lead to new pharmaceuticals. ..
  17. REGULATION OF GAD PROTEIN LEVELS AND GENE EXPRESSION
    Karin Rimvall; Fiscal Year: 1993
    ..expression of GAD67 and GAD65 is differentially regulated; GAD65 through its interaction with the co-factor pyridoxal phosphate and GAD67 at the level of protein expression...
  18. VITAMIN B-6 AND STERIOD HORMONE ACTION
    JOHN CIDLOWSKI; Fiscal Year: 1991
    ..Specifically we will continue to explore the modulatory role of the dietary cofactor Vitamin B6 (pyridoxal phosphate) in the mechanisms of glucocorticoid receptor action in cultured human cells...
  19. REACTION MECHANISMS FOR ENZYMES
    JULES SHAFER; Fiscal Year: 1990
    ....
  20. PERTURBATIONS IN VITAMIN B6 METABOLISM IN CANCER
    LOUISE NUTTER; Fiscal Year: 2000
    ....
  21. NUTRITIONAL AND BIOCHEMICAL/GENETIC MARKERS OF CANCER
    Jing Ma; Fiscal Year: 2007
    ..Hence, the proposed project is highly cost-efficient. ..
  22. HUMAN FUCOSYLTRANSFERASE STRUCTURE/FUNCTION ANALYSIS
    Eric Holmes; Fiscal Year: 2001
    ....
  23. REGULATION OF MAMMALIAN PYRIDOXAL PHOSPHATE METABOLISM
    MARGARET FONDA; Fiscal Year: 1980
    ..The possibility of product inhibition, feedback inhibition, and substrate activation of these enzymes will be studied to elucidate their role in control of vitamin B6 metabolism...
  24. VITAMIN B6 IN DEVELOPMENT OF CENTRAL NERVOUS SYSTEM
    Avanelle Kirksey; Fiscal Year: 1980
    ..Relative concentrations of pyridoxal phosphate and sequential changes in patterns of development on days 7, 12, 15, 21 and 50 will be investigated...
  25. 5-Aminolevulinate Synthase and Heme Biosynthesis
    Gloria Ferreira; Fiscal Year: 2006
    ..abstract_text> ..
  26. REGULATION TO FOLATE METABOLISM
    ROWENA MATTHEWS; Fiscal Year: 2000
    ..We will continue our studies on the catalytic mechanism of serine hydroxymethyltransferase, a pyridoxal phosphate-dependent enzyme that catalyzes the transfer of the beta-carbon of serine to H4folate to yield glycine and ..
  27. LONG ORAL CONTRACEPTIVE USE/VITAMIN B-6 PREGNANCY NEEDS
    Avanelle Kirksey; Fiscal Year: 1980
    ..effects of the different levels of vitamin B-6 supplementation, concentrations of total vitamin B-6 and pyridoxal phosphate will be measured in maternal plasma at 5 months gestation, at delivery and at 6 weeks postpartum and in ..
  28. High Flux X-ray Crystallography Data Collection System
    Harlan Wright; Fiscal Year: 2004
    ..metabolism, which are targets for new drug discovery in such diseases as tuberculosis; enzymes of pyridoxal phosphate and folate metabolism that are potential targets for new cancer chemotherapeutic agents and cardiovascular ..
  29. GYRATE ATROPHY OF THE CHOROID AND RETINA
    RICHARD WELEBER; Fiscal Year: 1980
    ..have been found to show in vitro responsiveness of cultured fibroblasts to increasing concentrations of pyridoxal phosphate. Five of the six have been evaluated for clinical responsiveness (lowering of elevated serum ornithine) and ..
  30. GLYCINE, SERINE AND THREONINE METABOLISM
    LEODIS DAVIS; Fiscal Year: 1990
    ..Many of these enzymes have as co-factors pyridoxal phosphate and/or folic acid. We will study the mechanism of catalysis by these enzymes...
  31. X-RAY STUDIES OF HEMOGLOBIN AND OTHER MACROMOLECULES
    Arthur Arnone; Fiscal Year: 1992
    ..in understanding the mechanism of action of this enzyme as well as of the large class of enzymes which used pyridoxal phosphate as a coenzyme...
  32. VITAMIN B-6 STATUS IN PREGNANCY, LACTATION AND INFANCY
    PHYLIS MOSER VEILLON; Fiscal Year: 1993
    ....
  33. ENZYME STEREOCHEMISTRY AND MECHANISM
    DONALD ABERHART; Fiscal Year: 1990
    ..In particular, the active site groups responsible for reported inhibitions by pyridoxal phosphate and by carbonyl-reactive reagents will be identified.
  34. CELLULAR CONTROL OF GLUTAMATE HOMEOSTASIS
    John Lehmann; Fiscal Year: 1993
    ..ii) Modulation of the alphaKG transporter by glutamate and by TAF require intracellular components (a pyridoxal phosphate-dependent enzyme and the endoplasmic reticulum, respectively)...
  35. ALCOHOL TOXICITY MEASURED BY MRI CONTRAST AGENTS
    Stuart Young; Fiscal Year: 1992
    ..Histology, plasma and tissue enzymes, and quantitative electron microscopic studies of vesicular transport mechanisms (using electron dense tracers) are to be obtained at appropriate intervals for comparison with the MRI/S095 studies...
  36. DETERMINATION OF FEMTOMOLES OF PYRIDOXAL PHOSPHATE
    BOB YANG; Fiscal Year: 1980
    ..No summary has been provided to the Smithsonian Science Information Exchange. ..
  37. MECHANISMS OF PYRIDOXAL-5'-PHOSPHATE DEPENDENT ENZYMES
    Robert Phillips; Fiscal Year: 2004
    ..Thus the results of this research are likely to make a significant contribution to the goal of improving health care for Americans in the future. ..
  38. Role of 10-formyldihydrofolate in purine biosynthesis
    Tsunenobu Tamura; Fiscal Year: 2004
    ..If this pathway is proven, derivatives of 10-HCO-H2folate could be used to inhibit de novo purine biosynthesis for developing new anticancer drugs. ..
  39. REACTION SPECIFICITY OF PYRIDOXAL PHOSPHATE ENZYMES
    MICHAEL TONEY; Fiscal Year: 2007
    b>Pyridoxal phosphate (PLP) dependent enzymes are ubiquitous in nitrogen metabolism and catalyze many medically important transformations. As a group, they catalyze an extraordinarily wide variety of reactions...
  40. Drug manipulation of noise-induced hearing loss
    RICHARD BOBBIN; Fiscal Year: 2002
    ....
  41. ENZYME CATALYSIS OF ELECTRON AND GROUP TRANSFER
    PERRY FREY; Fiscal Year: 2006
    ..The contributions of beta- amino acids produced by lysine 2,3-aminomutase and arginine 2,3-aminomutase to the functions of antibiotics are not known. The beta-aminoacids may become significant in drug development. ..
  42. Vitamin B12 and Folate in Relation to Cognition in the Framingham Offspring Study
    Jacob Selhub; Fiscal Year: 2010
    ..5 About 3-5 percent of seniors have low circulating vitamin B12 levels;6 however, about 20 percent of seniors are found to be deficient when those with elevated methylmalonic acid levels are included. ..
  43. Measurement of folate in fortified cereal grain products
    Jacob Selhub; Fiscal Year: 2003
    ..In order to assess the impact of folic acid fortification, it is necessary to have valid data on the actual synthetic folic acid content of fortified foods. ..
  44. FOOD FOLATE FORTIFICATION EFFECT ON FOLATE STATUS
    Jacob Selhub; Fiscal Year: 2001
    ..abstract_text> ..
  45. Folate, DNA Methylation and Breast Tumorigenesis
    Joshua Miller; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  46. Vitamin B12 Status of Elderly Americans
    MARTHA MORRIS; Fiscal Year: 2003
    ..The third aim is to evaluate the usefulness of a serum vitamin B12 cut-off point of 258 pmol/L, either as the sole indicator of vitamin B12 deficiency in the elderly, or in conjunction with anemia or an elevated metabolite level. ..
  47. MECHANISMS OF ENZYMATIC REACTIONS
    PERRY FREY; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  48. Ribozymes for new genetic coding systems
    John Richard; Fiscal Year: 2007
    ..abstract_text> ..
  49. B12 Absorption, Kinetics and Transcobalamin Genotype
    Joshua Miller; Fiscal Year: 2004
    ..This novel method would ultimately replace the Schilling test, the current gold standard, but flawed and cumbersome method for clinical assessment of B 12 absorptive capacity. ..
  50. Mechanisms of altered venous tone in hypertension
    ALEX PEREZ RIVERA; Fiscal Year: 2005
    ..Answers to these questions will provide a basic understanding of venous function that could lead to better pharmacological approaches not only for hypertension treatment but also for any disease in which there is a vein dysfunction. ..
  51. The TGFbeta Pathway, Gene Variants and Colorectal Neopl*
    Loic Le Marchand; Fiscal Year: 2005
    ..abstract_text> ..
  52. Macronutrients and Cardiovascular Risk
    Lawrence Appel; Fiscal Year: 2006
    ..abstract_text> ..
  53. Leak potassium channels in chemosensitive RTN neurons
    DANIEL MULKEY; Fiscal Year: 2006
    ..Results from these experiments will determine if TASK currents are present in pH-sensitive RTN neurons and provide insight into the cellular mechanism of mechanism chemoreception. [unreadable] [unreadable]..
  54. The Electrophysiology of Motor Neuron Diseases
    Mark Bromberg; Fiscal Year: 2002
    ..Currently, most MUNE techniques rely on proprietary software. We will develop software for use on PC-based computer systems, making them available to all laboratories. ..
  55. Gene and Nutrient Etiologies of Human Heart Defects
    Gary M Shaw; Fiscal Year: 2010
    ..Because these defects result in substantial morbidity as well as high emotional and economic costs, further scientific understanding leading to possible prevention would greatly benefit society. ..
  56. Hawaii Colorectal Cancer Family Registry
    Loic Le Marchand; Fiscal Year: 2008
    ..We will also propose a CFR pilot study exploring the feasibility of assessing assorted mating as evidence for population stratification. ..
  57. Effects of Light at Night on Circadian System in Nurses
    Eva Schernhammer; Fiscal Year: 2008
    ..abstract_text> ..
  58. PRESYNAPTIC MECHANISMS IN THE INTESTINE
    James Galligan; Fiscal Year: 2008
    ..It is also anticipated that these studies will identify new targets for drugs that can be used to treat GI motility disorders. ..
  59. Clock Genes, Night Work, and Breast Cancer Risk
    Eva Schernhammer; Fiscal Year: 2008
    ..The results from this study could help identify individuals who are more susceptible to night work than others, based on their genetic profile. ..
  60. MRI Evaluation of Acute Renal Transplant Dysfunction
    Vivian Lee; Fiscal Year: 2008
    ..At its conclusion, our study has a high likelihood of showing that functional MR renography for differentiating intrinsic renal diseases, will be an accurate tool for acute renal transplant dysfunction. ..