cation exchange resins

Summary

Summary: High molecular weight insoluble polymers which contain functional anionic groups that are capable of undergoing exchange reactions with cations.

Top Publications

  1. ncbi Solid-phase refolding of cyclodextrin glycosyltransferase adsorbed on cation-exchange resin
    Dae Hyuk Kweon
    Department of Agricultural Biotechnology, Seoul National University, Seoul 151 742, Korea
    Biotechnol Prog 20:277-83. 2004
  2. ncbi Cation exchange chromatography in antibody purification: pH screening for optimised binding and HCP removal
    Andreas Stein
    Merck KGaA, Frankfurter Strasse 250, 64293 Darmstadt, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 848:151-8. 2007
  3. ncbi Effects of ionic strength on lysozyme uptake rates in cation exchangers. I: Uptake in SP Sepharose FF
    S R Dziennik
    Department of Chemical Engineering, Center for Molecular and Engineering Thermodynamics, University of Delaware, Newark, Delaware 19716, USA
    Biotechnol Bioeng 91:139-53. 2005
  4. ncbi Sample clean-up method for analysis of complex-type N-glycans released from glycopeptides
    Miyako Nakano
    Faculty of Pharmaceutical Sciences, Kinki University, Kowakae 3 4 1, Higashi Osaka 577 8502, Japan
    J Chromatogr A 1005:13-21. 2003
  5. ncbi High-speed simultaneous ion-exclusion/cation-exchange chromatography of anions and cations on a weakly acidic cation-exchange resin column
    Masanobu Mori
    National Institute of Advanced Industrial Science and Technology at Seto, 110 Nishiibara cho, Seto, Aichi 489 0884, Japan
    J Chromatogr A 997:219-24. 2003
  6. ncbi Selection of pH-related parameters in ion-exchange chromatography using pH-gradient operations
    Tangir Ahamed
    Department of Biotechnology, Delft University of Technology, Delft, The Netherlands
    J Chromatogr A 1194:22-9. 2008
  7. ncbi Vacancy ion-exclusion chromatography of aromatic carboxylic acids on a weakly acidic cation-exchange resin
    Murad I H Helaleh
    National Institute of Advanced Industrial Science and Technology, Graduate School of Engineering, Chubu University, Seto 489 0884, Japan
    J Chromatogr A 997:139-44. 2003
  8. ncbi Vacancy ion-exclusion chromatography of haloacetic acids on a weakly acidic cation-exchange resin
    Murad I H Helaleh
    National Institute of Advanced Industrial Science and Technology, 110 Nishiibara cho, Seto, Aichi 489 0884, Japan
    J Chromatogr A 997:133-8. 2003
  9. ncbi Ion-exclusion chromatography with conductimetric detection of aliphatic carboxylic acids on a weakly acidic cation-exchange resin by elution with benzoic acid-beta-cyclodextrin
    Kazuhiko Tanaka
    National Institute of Advanced Industrial Science and Technology, 110 Nishiibara cho, Seto, Aichi 489 0884, Japan
    J Chromatogr A 997:127-32. 2003
  10. ncbi Monolithic column with double mixed-modes of hydrophilic interaction/cation-exchange and reverse-phase/cation-exchange stationary phase for pressurized capillary electrochromatography
    Jiabin Wang
    Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P R China
    Electrophoresis 29:928-35. 2008

Research Grants

  1. Synthetic Peptide Consensus Sequence Vaccine Development
    Robert Hodges; Fiscal Year: 2005
  2. LC/MS/MS Purchase
    Robert Hodges; Fiscal Year: 2003
  3. Analytical Ultracentrifuge Purchase
    Robert Hodges; Fiscal Year: 2006
  4. Alpbach Workshop:Coiled-Coils, Collagen/Co-proteins
    Robert Hodges; Fiscal Year: 2005
  5. HPLC and CE of Peptides and Proteins
    Robert S Hodges; Fiscal Year: 2010

Detail Information

Publications211 found, 100 shown here

  1. ncbi Solid-phase refolding of cyclodextrin glycosyltransferase adsorbed on cation-exchange resin
    Dae Hyuk Kweon
    Department of Agricultural Biotechnology, Seoul National University, Seoul 151 742, Korea
    Biotechnol Prog 20:277-83. 2004
    ..Taken together with the previous report in which a series of polycations were explored for efficient purification, expression of a target protein fused with a strong polycation provides a straightforward protein preparation scheme...
  2. ncbi Cation exchange chromatography in antibody purification: pH screening for optimised binding and HCP removal
    Andreas Stein
    Merck KGaA, Frankfurter Strasse 250, 64293 Darmstadt, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 848:151-8. 2007
    ..With this pH we ran labscale columns to purify monoclonal antibody. The cation exchangers showed high step yields and host cell proteins in the pools from gradient elution were reduced very effectively...
  3. ncbi Effects of ionic strength on lysozyme uptake rates in cation exchangers. I: Uptake in SP Sepharose FF
    S R Dziennik
    Department of Chemical Engineering, Center for Molecular and Engineering Thermodynamics, University of Delaware, Newark, Delaware 19716, USA
    Biotechnol Bioeng 91:139-53. 2005
    ..In addition, the results provide heuristics for the optimization of chromatographic conditions...
  4. ncbi Sample clean-up method for analysis of complex-type N-glycans released from glycopeptides
    Miyako Nakano
    Faculty of Pharmaceutical Sciences, Kinki University, Kowakae 3 4 1, Higashi Osaka 577 8502, Japan
    J Chromatogr A 1005:13-21. 2003
    ..In the model study presented here, we were able to obtain N-glycans for HPAEC analyses without any interfering materials by a combination of TNBS reaction and graphitized carbon treatment...
  5. ncbi High-speed simultaneous ion-exclusion/cation-exchange chromatography of anions and cations on a weakly acidic cation-exchange resin column
    Masanobu Mori
    National Institute of Advanced Industrial Science and Technology at Seto, 110 Nishiibara cho, Seto, Aichi 489 0884, Japan
    J Chromatogr A 997:219-24. 2003
    ..36 to 0.68 microM for anions and 0.63-0.99 microM for cations. This method has been applied to the simultaneous determination of anions and cations in several environmental waters with satisfactory results...
  6. ncbi Selection of pH-related parameters in ion-exchange chromatography using pH-gradient operations
    Tangir Ahamed
    Department of Biotechnology, Delft University of Technology, Delft, The Netherlands
    J Chromatogr A 1194:22-9. 2008
    ..5 or anion-exchange chromatography (AEX) at pH 7.2 without lowering the salt concentration in the CCS. The performance of both CEX and AEX was predicted to be equal for this particular MAb capture...
  7. ncbi Vacancy ion-exclusion chromatography of aromatic carboxylic acids on a weakly acidic cation-exchange resin
    Murad I H Helaleh
    National Institute of Advanced Industrial Science and Technology, Graduate School of Engineering, Chubu University, Seto 489 0884, Japan
    J Chromatogr A 997:139-44. 2003
    ..66, 0.67, 0.42 and 0.86 microM and detector responses were linear in the range 1-100, 1-80, 2.5-100 and 10-40 microM, for SA, NTCA, PA and BA, respectively. Precision for retention times was 0.36% and for peak areas was 1.5%...
  8. ncbi Vacancy ion-exclusion chromatography of haloacetic acids on a weakly acidic cation-exchange resin
    Murad I H Helaleh
    National Institute of Advanced Industrial Science and Technology, 110 Nishiibara cho, Seto, Aichi 489 0884, Japan
    J Chromatogr A 997:133-8. 2003
    ..The detection limits of these acids in the present study with conductivity detection were 3.4 microM for monochloroacetic acid, 0.86 microM for dichloroacetic acid and 0.15 microM for trichloroacetic acid...
  9. ncbi Ion-exclusion chromatography with conductimetric detection of aliphatic carboxylic acids on a weakly acidic cation-exchange resin by elution with benzoic acid-beta-cyclodextrin
    Kazuhiko Tanaka
    National Institute of Advanced Industrial Science and Technology, 110 Nishiibara cho, Seto, Aichi 489 0884, Japan
    J Chromatogr A 997:127-32. 2003
    ..The ion-exclusion chromatographic separation of carboxylic acids with high resolution and sensitivity was accomplished successfully by elution with a 1 mM benzoic acid-10 mM cyclodextrin solution without chemical suppression...
  10. ncbi Monolithic column with double mixed-modes of hydrophilic interaction/cation-exchange and reverse-phase/cation-exchange stationary phase for pressurized capillary electrochromatography
    Jiabin Wang
    Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P R China
    Electrophoresis 29:928-35. 2008
    ..Effects of salt concentration and ACN content on the separation have also been investigated. High column efficiencies of up to 352 000 plates/meter were obtained by the separation of narcotic pharmaceuticals...
  11. ncbi Simultaneous determination of cations, zwitterions and neutral compounds using mixed-mode reversed-phase and cation-exchange high-performance liquid chromatography
    Jingyi Li
    Analytical Research and Development, Celgene Corporation, Summit, NJ 07091, USA
    J Chromatogr A 1185:185-93. 2008
    ..Moreover, the method performance characteristics of selectivity, sensitivity and accuracy have been demonstrated to be suitable to determine the related impurities in the capsules of Drug A...
  12. ncbi High-performance cation-exchange chromatofocusing of proteins
    Xuezhen Kang
    Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA
    J Chromatogr A 991:117-28. 2003
    ....
  13. ncbi Theory and applications of a novel ion exchange chromatographic technology using controlled pH gradients for separating proteins on anionic and cationic stationary phases
    Latchezar I Tsonev
    CryoBioPhysica, Inc, Biomedical Research Institute, 12111 Parklawn Drive, Rockville, MD 20852, USA
    J Chromatogr A 1200:166-82. 2008
    ....
  14. ncbi Preparative weak cation-exchange chromatography of monoclonal antibody variants I. Single-component adsorption
    Lena Melter
    Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, 8093 Zurich, Switzerland
    J Chromatogr A 1200:156-65. 2008
    ..A correlation describing the effect of the separation conditions on the diffusion rate is therefore needed in order to have a fully predictive mathematical model...
  15. ncbi Co-eluent effect in partition chromatography. Rhamnose-xylose separation with strong and weak cation-exchangers in aqueous ethanol
    Jari Tiihonen
    Lappeenranta University of Technology, Laboratory of Industrial Chemistry, P O Box 20, FIN 53851 Lappeenranta, Finland
    J Chromatogr A 982:69-84. 2002
    ..The experimental data were successfully explained with a rate-based column model, which accounted for the volume changes of the stationary phase. A thermodynamic sorption model was utilized in column calculations...
  16. ncbi Gravity-flow open tubular cation chromatography
    Petr Kuban
    Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, Brno, Czech Republic
    J Sep Sci 31:2745-53. 2008
    ..75 mM pyridinedicarboxylic acid as an eluent. Contactless conductometric detection (C(4)D) allows LODs down to 150 nmol/L, corresponding to 30 fmol injections. Analysis of real water samples is demonstrated...
  17. ncbi Integrated strategy for selective expanded bed ion-exchange adsorption and site-specific protein processing using gene fusion technology
    Torbjörn Gräslund
    Department of Biotechnology, Royal Institute of Technology KTH, SCFAB, S 106 91, Stockholm, Sweden
    J Biotechnol 96:93-102. 2002
    ..The strategy also includes an integrated site-specific removal of the Z(basic) purification handle to yield a free target protein...
  18. ncbi pH Transitions in ion-exchange systems: role in the development of a cation-exchange process for a recombinant protein
    Sanchayita Ghose
    Product Recovery, Immunex Corporation, 51 University Street, Seattle, Washington 98101, USA
    Biotechnol Prog 18:530-7. 2002
    ..The results from this study were applied to the cation-exchange step for a recombinant protein that was sensitive to pH excursions to help maintain activity of the protein during the purification process...
  19. ncbi Mass transfer effects in preparative chromatography of eremomycin on polymeric sorbents
    Irina V Polyakova
    Department of Biopolymers, Institute of Molecular Compounds, Russian Academy of Sciences, Bolshoi pr 31, St Petersburg, Russia
    J Chromatogr A 1006:251-60. 2003
    ..The optimal conditions for sorption and complete desorption of eremomycin were found...
  20. ncbi Automated injection of uncleaned samples using a ten-port switching valve and a strong cation-exchange trap column for proteome analysis
    Fangjun Wang
    National Chromatographic R and A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
    J Chromatogr A 1171:56-62. 2007
    ....
  21. ncbi Preparation and characterization of prototypes for multi-modal separation aimed for capture of positively charged biomolecules at high-salt conditions
    Bo Lennart Johansson
    Research and Development, Amersham Biosciences, SE 751 84, Uppsala, Sweden
    J Chromatogr A 1016:35-49. 2003
    ..Varying the ligand ratio (SP/Phenyl) could be used for optimizing the function of mixed-ligand ion-exchange media...
  22. ncbi Enrichment and identification of cysteine-containing peptides from tryptic digests of performic oxidized proteins by strong cation exchange LC and MALDI-TOF/TOF MS
    Jingquan Dai
    Beijing Institute of Radiation Medicine, China
    Anal Chem 77:7594-604. 2005
    ..The strength of the strategy was demonstrated by applying it to bovine serum albumin. Potential use of the strategy in proteomics was also discussed...
  23. ncbi Influence of acidic eluent for retention behaviors of common anions and cations by ion-exclusion/cation-exchange chromatography on a weakly acidic cation-exchange resin in the H+ -form
    Masanobu Mori
    Faculty of Engineering, Gunma University, Kiryu, Gunma 376 8515, Japan
    J Chromatogr A 1118:51-5. 2006
    ..1-2.9% for anions and 1.8-4.5% for cations. This method was successfully applied to hot spring water containing strong acid anions, weak acid anions and cations, with satisfactory results...
  24. ncbi [Studies of chromatographic behaviors of proteins on synthesized cation-exchanger]
    Rong Li
    School of Life Science and Technology, Xi an Jiaotong University, Xi an 710049, China
    Se Pu 22:134-7. 2004
    ..The interaction between chelating weak cation-exchanger and metal ions was examined. The retention mechanism of protein in metal chelating chromatography was explored...
  25. ncbi [Adsorption condition optimization for anti-HBsAg Fab fragment separation and purification from E. coli using Streamline SP]
    Yu Xian Huang
    Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
    Nan Fang Yi Ke Da Xue Xue Bao 26:409-13. 2006
    ..To optimize the adsorption condition of cation-exchange chromatographic media Streamline SP for separation and purification of anti-HBsAg Fab fragment from E. coli...
  26. ncbi Role of zwitterionic structures in the solid-phase extraction based method development for clean up of tetracycline and oxytetracycline from honey
    Shahana Huq
    Phenomenex, Inc, 411 Madrid Ave, Torrance, CA 90501, USA
    J Chromatogr A 1135:12-8. 2006
    ....
  27. ncbi Effect of spacer arm length on protein retention on a strong cation exchange adsorbent
    Peter DePhillips
    Merck Research Laboratories, Sumneytown Road, West Point, PA 19486, USA
    Anal Chem 76:5816-22. 2004
    ..The results indicate that spacer arm length may be a useful variable in manipulating stationary-phase retention properties...
  28. ncbi Protein separations with induced pH gradients using cation-exchange chromatographic columns containing weak acid groups
    Timothy M Pabst
    Department of Chemical Engineering, University of Virginia, Charlottesville, VA 22904 4741, USA
    J Chromatogr A 1181:83-94. 2008
    ....
  29. ncbi Ion exchange chromatography of antibody fragments
    Anders Ljunglöf
    GE Healthcare, Bio Sciences AB, SE 751 84 Uppsala, Sweden
    Biotechnol Bioeng 96:515-24. 2007
    ..Such results are in agreement with recent studies related to ion exchange of whole antibody molecules under similar conditions...
  30. ncbi Relative retention of the fibroblast growth factors FGF-1 and FGF-2 on strong cation-exchange sorbents
    Peter DePhillips
    Merck Research Laboratories, Sumneytown Road, West Point, PA 19486, USA
    J Chromatogr A 1036:51-60. 2004
    ..These observations are interpreted in terms of the structural features of the proteins and the physicochemical properties of the adsorbents...
  31. ncbi Cation exchangers for selective sorption of large proteins
    O V Ochkur
    Institute of Macromolecular Compounds, Russian Academy of Science, Bolshoi pr 31, St Petersburg, Russian Federation
    J Chromatogr B Analyt Technol Biomed Life Sci 849:231-5. 2007
    ..The dependence of sorption selectivity on protein size and on concentration of ionogenic groups was demonstrated...
  32. ncbi Parallel screening of selective and high-affinity displacers for proteins in ion-exchange systems
    Kaushal Rege
    Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA
    J Chromatogr A 1033:19-28. 2004
    ..The results presented in this paper demonstrate that this parallel batch screening-QSER approach can be employed for the identification of selective and high-affinity displacers for protein mixtures...
  33. ncbi Application of a chromatography model with linear gradient elution experimental data to the rapid scale-up in ion-exchange process chromatography of proteins
    Takashi Ishihara
    CMC R and D Laboratories, Pharmaceutical Division, Kirin Brewery Co Ltd, Takasaki, Gunma 370 0013, Japan
    J Chromatogr A 1162:34-40. 2007
    ..Resolution, recovery and purity were examined in order to verify the proposed method...
  34. ncbi Rational method for designing efficient separations by chromatography on polystyrene-divinylbenzene resins eluted with aqueous ethanol
    Shuichi Yamamoto
    Laboratory of Bioprocess Engineering, School of Engineering and Graduate School of Medicine, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
    J Chromatogr A 1162:50-5. 2007
    ..Stepwise elution experiments were also designed and performed successfully on the basis of the K-I curve...
  35. ncbi Simultaneous determination of N-oxides and free bases of pyrrolizidine alkaloids by cation-exchange solid-phase extraction and ion-pair high-performance liquid chromatography
    Tomasz Mroczek
    Department of Pharmacognosy, Medical University, Lublin, Poland
    J Chromatogr A 949:249-62. 2002
    ..For the last three samples macrocyclic PAs (senecionine, senecionine-N-oxide. senkirkine) have been detected for the first time. Details of precision of the analyses are also included...
  36. ncbi Influence of column type and chromatographic conditions on the ion-exchange chromatography of immunoglobulins
    Y B Yang
    Separations Group, Inc, Hesperia, CA 92345, USA
    J Chromatogr A 743:171-80. 1996
    ..Significant differences in the chromatographic behavior (elution pattern, peak shape and selectivity) of the test samples are discussed in regard to the column type and the chromatographic conditions...
  37. ncbi Determinants of protein retention characteristics on cation-exchange adsorbents
    P DePhillips
    Merck Research Laboratories, Sumneytown Rd, West Point, PA 19486, USA
    J Chromatogr A 933:57-72. 2001
    ..Protein retention was increased on adsorbents with pore size distributions that include significant amounts of pore space with dimensions similar to those of the protein solute...
  38. ncbi Characterisation of the novel restriction endonuclease SuiI from Sulfolobus islandicus
    Stefan Söllner
    Institute of Biochemistry, University of Bayreuth, Universitatstrasse 30, 95447 Bayreuth, Germany
    Extremophiles 10:629-34. 2006
    ..The cleavage occurs after the first guanosine base and is inhibited by 5-methyl-cytosine methylation. The restriction activity is salt-sensitive and has an optimal activity around 70 degrees C...
  39. ncbi Characterization and application of strong ion-exchange membrane adsorbers as stationary phases in high-performance liquid chromatography of proteins
    O W Reif
    , , Germany
    J Chromatogr A 654:29-41. 1993
    ..Appropriate conditions were defined for the separation of human serum and for the isolation of subtilisin Carlsberg and beta-galactosidase from cell culture supernatants...
  40. ncbi Comparison of protein adsorption isotherms and uptake rates in preparative cation-exchange materials
    C Chang
    Department of Chemical Engineering, University of Delaware, Newark 19716, USA
    J Chromatogr A 827:281-93. 1998
    ..Additional information is required to elucidate further the mechanisms of coupled diffusion and adsorption in stationary phases...
  41. ncbi Synthesis of monodisperse poly(chloromethylstyrene-co-divinylbenzene) beads and their application in separation of biopolymers
    Bolin Gong
    Key Laboratory of Energy and Chemical Engineering, Ningxia University, Yinchuan 750021, P R China
    J Sep Sci 28:2546-50. 2005
    ..0 min with only one step. The purity and specific bioactivity of the purified lysozyme were found to be more than 93% and 70 245 U/mg, respectively...
  42. ncbi Protein adsorption on novel acrylamido-based polymeric ion-exchangers. I. Morphology and equilibrium adsorption
    A K Hunter
    Department of Chemical Engineering, University of Virginia, Charlottesville 22903-2442, USA
    J Chromatogr A 897:65-80. 2000
    ..For these ion-exchangers, high protein adsorption capacities are attained when a low-density polymer gel with a high concentration of functional groups is present...
  43. ncbi Simplified in-line sample preparation for amino acid analysis in carbohydrate containing samples
    P Jandik
    Dionex Corporation, Research and Development, Sunnyvale, CA 94088-3603, USA
    J Chromatogr B Biomed Sci Appl 758:189-96. 2001
    ..99. The mean value of relative standard deviations from five replicates was 3.9%. The usefulness of the method is illustrated with two chromatograms of a carrot juice sample obtained before and after the in-line removal of carbohydrates...
  44. pmc Volumetric interpretation of protein adsorption: ion-exchange adsorbent capacity, protein pI, and interaction energetics
    Hyeran Noh
    Department of Materials Science and Engineering, Huck Institute of Life Sciences and Materials Research Institute, The Pennsylvania State University, University Park, PA 16802, USA
    Biomaterials 29:2033-48. 2008
    ....
  45. ncbi Analysis of the physicochemical interactions between Clostridium difficile toxins and cholestyramine using liquid chromatography with post-column derivatization
    G P Palace
    Analytical Chemistry Department, GelTex Pharmaceuticals, Inc, 153 Second Avenue, Waltham, MA 02451, USA
    Biochim Biophys Acta 1546:171-84. 2001
    ..Binding to strong cation exchange resins (sulfonate-PHM) was not observed...
  46. ncbi [Multiple type I pseudohypoaldosteronism: neonatal management and outcome]
    M Cristina De Frutos Martínez
    Servicio de Neonatologia, Hospital Infantil La Paz, Universidad Autonoma de Madrid
    An Esp Pediatr 52:47-51. 2000
    ..The treatment consisted of salt and sodium bicarbonate supplements, restricted potassium intake, cation exchange resins and high fluid intake. During first year she was hospitalized for severe salt-losing crises...
  47. ncbi Identification of S-methylmethionine in Petit Manseng grapes as dimethyl sulphide precursor in wine
    N Loscos
    Dpto De Química Analítica Facultad de Ciencias Universidad de Zaragoza, C Pedro Cerbuna no 12, 50009 Zaragoza, Spain
    Anal Chim Acta 621:24-9. 2008
    ..SMM peak identification and quantification (401.2nmolmL(-1) relatively to norleucine used as internal standard) were carried out by comparison with commercial SMM...
  48. pmc Effect of polysulfonate resins and direct compression fillers on multiple-unit sustained-release dextromethorphan resinate tablets
    Thaned Pongjanyakul
    Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
    AAPS PharmSciTech 6:E190-7. 2005
    ..DMP resinates were formed by a complexation of DMP and strong cation exchange resins, Dowex 50 W and Amberlite IRP69...
  49. ncbi On-line cation exchange for suppression of adduct formation in negative-ion electrospray mass spectrometry of nucleic acids
    C G Huber
    Institute of Analytical Chemistry and Radiochemistry, Leopold Franzens University, Innsbruck, Austria
    Anal Chem 70:5288-95. 1998
    ..2-20 microM with selected-ion monitoring. Finally, the on-line sample preparation system was evaluated for the mass spectrometric analysis of complex oligonucleotide mixtures...
  50. ncbi Preparation of phytate-removed deamidated soybean globulins by ion exchangers and characterization of their calcium-binding ability
    Hitomi Kumagai
    Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa shi 252 8510, Japan
    J Agric Food Chem 50:172-6. 2002
    ..10 g/mL of CE-4 in a 0.2% soybean globulin solution for 6 h at 4 degrees C. The amount of calcium bound to the soybean globulins decreased with removal of the phytate but increased with deamidation...
  51. ncbi Formulation design of ranitidine hydrochloride to reduce its moisture absorption characteristics
    Shagufta Khan
    Department of Pharmaceutics, Institute of Pharmaceutical Education and Research, Borgaon Meghe Wardha, Maharashtra, India
    PDA J Pharm Sci Technol 63:226-33. 2009
    ..Drug resin complexes (DRCs) were prepared using the batch method with (i) two weak cation exchange resins, Polacrilex with exchangeable H+ and Polacrillin potassium; and (ii) a strong cation exchange resin;Sodium ..
  52. ncbi Selective factor VIII and V inactivation by iminodiacetate ion exchange resin through metal ion adsorption
    Masahiro Takeyama
    Department of Paediatrics, Nara Medical University, Kashihara, Nara, Japan
    Br J Haematol 142:962-70. 2008
    ..Furthermore, iminodiacetate resin-treated plasma would be useful as a substrate for measuring the activity of these factors...
  53. ncbi [13C] GC-C-IRMS analysis of methylboronic acid derivatives of glucose from liver glycogen after the ingestion of [13C] labeled tracers in rats
    Catherine Luengo
    INRA, CNRH IdF, UMR914 Nutrition Physiology and Ingestive Behavior, F 75005 Paris, France
    J Chromatogr B Analyt Technol Biomed Life Sci 877:3638-44. 2009
    ..Hepatic glycogen was extracted, digested to glucose and purified on anion-cation exchange resins. After the optimization of methylboronic acid derivatization using GC-MS, [(13)C] enrichment of extracted ..
  54. ncbi Anion chromatography using on-line recycled eluents
    Takashi Yokoyama
    Department of Chemistry, Faculty of Science, Okayama University of Science, 1 1 Ridai cho, Okayama 700 0005, Japan
    J Chromatogr A 1089:82-6. 2005
    ..The performance of the anion chromatography, using on-line recycled eluents, was the same as that for normal anion chromatography under the same conditions...
  55. ncbi Combined ion exchange treatment for removal of dissolved organic matter and hardness
    Jennifer N Apell
    Department of Environmental Engineering Sciences, University of Florida, P O Box 116450, Gainesville, FL 32611 6450, USA
    Water Res 44:2419-30. 2010
    ..Hardness ions can be removed with cation exchange resins, although typically using a fixed bed reactor at the end of a treatment train...
  56. ncbi Effect of phytate-removal and deamidation of soybean proteins on calcium absorption in the in situ rats
    Hitomi Kumagai
    Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa shi 252 8510, Japan
    Biofactors 22:21-4. 2004
    ..As a result, PrDS retained their high calcium binding properties even after hydrolysis by digestive enzymes. In situ experiments showed that PrDS and their hydrolysates enhanced the calcium absorption from the intestine...
  57. ncbi Highly efficient removal of heavy metals by polymer-supported nanosized hydrated Fe(III) oxides: behavior and XPS study
    Bingjun Pan
    State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Nanjing 210093, PR China
    Water Res 44:815-24. 2010
    ..More attractively, the exhausted HFO-001 beads can be effectively regenerated by HCl-NaCl solution (pH 3) for repeated use without any significant capacity loss...
  58. ncbi Simple and rapid analytical method for detection of amino acids in blood using blood spot on filter paper, fast-GC/MS and isotope dilution technique
    Shuichi Kawana
    Department of Pediatrics, Shimane University School of Medicine, 89 1 En ya cho, Izumo, Shimane 693 8501, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 878:3113-8. 2010
    ..These results indicate that this method provides a simple and rapid procedure for precise determination of amino acids in whole blood...
  59. ncbi Control of plant diseases with secondary metabolite of Clitocybe nuda
    Jin Tong Chen
    Department of Plant Pathology, National Chung Hsing University, Taichung 402, Taiwan
    N Biotechnol 26:193-8. 2009
    ..It was also exchangeable by anion but not cation exchange resins, indicating that the inhibitor has a molecular weight between 1000 and 500, and negative charge on its ..
  60. pmc Ion exchange chromatography of monoclonal antibodies: effect of resin ligand density on dynamic binding capacity
    Ann Marie Hardin
    Genentech Inc, South San Francisco, CA 94080, USA
    J Chromatogr A 1216:4366-71. 2009
    Dynamic binding capacity (DBC) of a monoclonal antibody on agarose based strong cation exchange resins is determined as a function of resin ligand density, apparent pore size of the base matrix, and protein charge...
  61. ncbi Coadsorption of trivalent metal ions and anions on strongly acidic cation-exchange resins by bridge bonding
    Takanori Matsuura
    Graduate School of Engineering, Nagoya Institute of Technology, Gokiso, Showa, Nagoya, 466 8555, Japan
    Anal Chem 80:9666-71. 2008
    ..These findings indicate that the bridge bonding of anions between the metal ions adsorbed on the resins of the higher exchange capacities plays a crucial role for the coadsorption. Some analytical implication was also discussed...
  62. ncbi Extraction of extracellular polymeric substances from extreme acidic microbial biofilms
    Angeles Aguilera
    Centro de Astrobiología INTA CSIC, Madrid, Spain
    Appl Microbiol Biotechnol 78:1079-88. 2008
    ..8 and Crown Ether cation exchange resins) were tested...
  63. ncbi Role of the ligand density in cation exchange materials for the purification of proteins
    Agnes Franke
    Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland
    J Chromatogr A 1217:2216-25. 2010
    The performance of functionalized materials, such as cation exchange resins, is dependent not only on the ligand type and ligand density, but also on the pore accessibility of the target molecule...
  64. ncbi Sample preparation and in-solution protease digestion of proteins for chromatography-based proteomic analysis
    Michael P Washburn
    Stowers Institute for Medical Research, Kansas City, Missouri, USA
    Curr Protoc Protein Sci . 2008
    ..are directly loaded onto a microcapillary column packed sequentially with reversed-phase and strong cation exchange resins. Once digested protein samples are loaded onto the column, the column is placed in-line between a high-..
  65. pmc Formulation and In vitro Evaluation of Sustained Release Dosage Form with Taste Masking of Metformin Hydrochloride
    P K Bhoyar
    S K B College of Pharmacy, New Kamptee, Nagpur 441 002, India
    Indian J Pharm Sci 72:184-90. 2010
    ..Results showed that metformin HCl was successfully taste masked and formulated into a sustained dosage form as an alternative to the conventional tablet...
  66. ncbi Selection and evaluation of adsorbents for the removal of anionic surfactants from laundry rinsing water
    Natasja Schouten
    Wetsus, Centre for Sustainable Water Technology, Agora 1, PO Box 1113, 8900 CC Leeuwarden, The Netherlands
    Water Res 41:4233-41. 2007
    ..02-0.6 gLAS/g. Negatively charged materials, such as cation exchange resins or bentonite clay, have negligible adsorption capacities for LAS...
  67. ncbi Cadmium sorption characteristics of phosphorylated sago starch-extraction residue
    Masato Igura
    Graduate School of Bio Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 2 24 16 Nakacho, Koganei, Tokyo 184 8588, Japan
    J Hazard Mater 178:686-92. 2010
    ..2 mg g(-1), which is almost half the capacity of commercially available weakly acidic cation exchange resins. The phosphorylated residue could be reused several times, although cadmium sorption gradually decreased ..
  68. ncbi Removal of Pb from a calcareous soil during EDTA-enhanced electrokinetic extraction
    S Amrate
    Laboratory of Hydrometallurgy and Molecular Inorganic Chemistry, Faculty of Chemistry, University of Science and Technology Houari Boumediene USTHB, Bab Ezzouar, Algiers, Algeria
    Sci Total Environ 349:56-66. 2005
    ..Thus, simultaneous recovery of EDTA and lead from their chelated solutions has been made possible using the same set-up and by controlling fluids chemistry...
  69. ncbi Understanding medication errors: discussion of a case involving a urinary catheter implicated in a wrong route error
    Rodney W Hicks
    Texas Tech University Health Sciences Center, School of Nursing, Lubbock, TX, USA
    Urol Nurs 28:454-9. 2008
    ..Awareness of national trends for patient safety, including emerging changes leading to best practices, updates to National Patient Safety Goals, and changes in national policy, can reduce the risk of error involvement...
  70. ncbi Dedicated removal of immunoglobulin (Ig)A, IgM, and Factor (F)XI/activated FXI from human plasma IgG
    Yu Wen Wu
    Institute of Medical Biomaterials and Tissue Engineering, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan Research and Development, Chromatography Applications, Pall Life Sciences, Cergy, France Pall Life Sciences, Northborough, Massachusetts Human Protein Process Sciences HPPS, Lille, France
    Transfusion 54:169-78. 2014
    ..Some adverse events are due to contaminants like IgA and activated Factor (F)XI. Therefore, new purification strategies are needed for dedicated removal of these contaminants without impairing IgG recovery...
  71. ncbi Development of an online two-dimensional nano-scale liquid chromatography/mass spectrometry method for improved chromatographic performance and hydrophobic peptide recovery
    Hongji Liu
    Waters Corporation, Milford, MA 01757, USA
    J Chromatogr A 1135:43-51. 2006
    ..coli peptides identified from the RP fractions. These peptides, which were 19% of all E. coli peptides identified from a 2D run, would not have been identified without the application of the RP gradient to the SCX column...
  72. ncbi Simultaneous determination of creatinine, creatine, and UV-absorbing amino acids using dual-mode gradient low-capacity cation-exchange chromatography
    Yukio Yokoyama
    Department of Analytical Chemistry, Faculty of Engineering, Yokohama National University, Tokiwadai, Hodogaya, Yokohama 240 8501, Japan
    J Chromatogr A 1085:110-6. 2005
    ..The developed method is very practical and can provide useful information and results for the clinical or biomedical researches with low analytical run costs...
  73. ncbi Cation exchange/hydrophobic interaction monolithic chromatography of small molecules and proteins by nano liquid chromatography
    Cemil Aydoğan
    Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey
    J Sep Sci 36:1685-92. 2013
    ..Theoretical plate number up to 52,000 plates/m was successfully achieved. The prepared monolith was further applied to the proteins with different acetonitrile content...
  74. ncbi Zr and U determination at trace level in simulated deep groundwater by Q ICP-MS using extraction chromatography
    C Gautier
    Commissariat à l Énergie Atomique et aux Énergies Alternatives, CEA Saclay, DEN DANS DPC SEARS LASE, Batiment 459, PC 171, 91191 Gif sur Yvette Cedex, France
    Talanta 106:1-7. 2013
    ..Compared to a sample dilution implemented to reduce the matrix effects, the developed preconcentration method allowed improving the sensitivity up to a 20 fold factor for Zr and U measurements at trace level by Q ICP-MS...
  75. ncbi Comparison of gaseous oxidized Hg measured by KCl-coated denuders, and nylon and cation exchange membranes
    Jiaoyan Huang
    Department of Natural Resources and Environmental Sciences, University of Nevada, Reno, 1664, N Virginia Street, Reno, Nevada 89557, United States
    Environ Sci Technol 47:7307-16. 2013
    ..Understanding the chemistry of GOM across space and time has important implications for those developing policy regarding this environmental contaminant. ..
  76. ncbi Preparation, characterization, and process performance of composite fibrous adsorbents as cation exchangers for high throughput and high capacity bioseparations
    Poondi Rajesh Gavara
    Downstream Processing Laboratory, Jacobs University Bremen gGmbH, Campus Ring 1, D 28759 Bremen, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 903:14-22. 2012
    ..5% yeast homogenate. Summarizing, the composite fibrous adsorbents exhibited superior performance during early protein capture and intermediate-resolution applications...
  77. ncbi Improved N(α)-acetylated peptide enrichment following dimethyl labeling and SCX
    Sin Hong Chen
    Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung, Taiwan
    J Proteome Res 12:3277-87. 2013
    ..Compared to other methods, this method is relatively simple and can be directly implemented in a two-dimensional separation (SCX-RP)-mass spectrometry scheme for quantitative N-terminal proteomics using stable-isotope dimethyl labeling. ..
  78. ncbi Amberlyst-15 and Amberlite-200C: efficient catalysts for aldol and cross-aldol condensation under ultrasound irradiation
    Achraf Lahyani
    Laboratory of Applied Chemistry Heterocyclics, Fats and Polymers, Faculty of Sciences, University of Sfax, Route de soukra Km 3, 5, 3038 Sfax, Tunisia
    Ultrason Sonochem 20:1296-301. 2013
    ..Several trans-chalcones and α,α'-bis(arylmethylidene) cycloalkanones were synthesized in good yields and excellent selectivity in a short reaction time...
  79. ncbi Application of imaging capillary IEF for characterization and quantitative analysis of recombinant protein charge heterogeneity
    Zoran Sosic
    Analytical Development, Biogen Idec, Cambridge, MA 02142, USA
    Electrophoresis 29:4368-76. 2008
    ..Some aspects of the method specificity for use as an identity test in biopharmaceutical development have been discussed...
  80. ncbi Ammonium removal from anaerobic digester effluent by ion exchange
    T Wirthensohn
    Department of IFA Tulln, Institute for Environmental Biotechnology, University of Natural Resources and Applied Life Sciences Vienna, Konrad Lorenz Strasse 20, 3430 Tulln, Austria
    Water Sci Technol 60:201-10. 2009
    ..74 mg/l; Ca2+= 1.79 mg/l). The aim of this study was to remove this ammonium by ion exchange. Acidic gel cation exchange resins and clinoptilolite were tested in column experiments to evaluate their capacity, flow rates and pH...
  81. ncbi [Sulfonation modification-assisted enrichment and identification of histidine-containing peptides by strong cation exchange chromatography and mass spectrometry]
    Dong Cao
    Beijing Institute of Technology, Beijing 100081, China
    Se Pu 27:158-63. 2009
    ..It is feasible to isolate and enrich the histidine-containing peptides by using this method which has the potential applications in proteomics...
  82. ncbi iTRAQ experimental design for plasma biomarker discovery
    Xiaomin Song
    Australian Proteome Analysis Facility Ltd, Macquarie University, Sydney, Australia
    J Proteome Res 7:2952-8. 2008
    ..Our findings are discussed in the context of optimizing iTRAQ experimental design for robust plasma-based biomarker discovery...
  83. doi Preparation of soluble proteins from Escherichia coli
    Paul T Wingfield
    National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Protein Sci . 2005
    ..The purification protocol described is typical for a protein that is expressed in fairly high abundance (i.e., >5% total protein) and accumulates in a soluble state...
  84. ncbi A high yield multi-method extraction protocol for protein quantification in activated sludge
    Ras Monique
    Laboratoire de Biologie appliquée à l Agro alimentaire et à l Environnement, Institut Universitaire de Technologie, Universite Toulouse III, 24 Rue d Embaquès, 32000 Auch, France
    Bioresour Technol 99:7464-71. 2008
    ..Thus, further qualitative studies may not be biased by the extraction protocol...
  85. ncbi Cation exchange resins as pharmaceutical carriers for methylene blue: binding and release
    Felix Gut
    Department of Internal Medicine VI, University of Heidelberg, Germany
    Eur J Pharm Biopharm 69:582-7. 2008
    ..The binding to seven cation exchange resins was studied. Ion exchangers on acrylic or methacrylic acid basis bound between 1.54 and 2...
  86. ncbi Pre-concentration of trace elements in short chain alcohols using different commercial cation exchange resins prior to inductively coupled plasma-optical emission spectrometric detection
    Philiswa N Nomngongo
    Department of Applied Chemistry, University of Johannesburg, Johannesburg, 2028, South Africa
    Anal Chim Acta 787:78-86. 2013
    ..The optimized method was applied to the separation and pre-concentration of metal ions in different commercial alcohol samples...
  87. ncbi Debromination of decabrominated diphenyl ether by resin-bound iron nanoparticles
    An Li
    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing, China
    Environ Sci Technol 41:6841-6. 2007
    ....
  88. ncbi Recovery of nickel ions from simulated electroplating rinse water by electrodeionization process
    H Lu
    College of Environmental Science and Engineering, Nankai University, Nankai District, 300071 Tianjin, China
    Water Sci Technol 61:729-35. 2010
    ..7. The experiment results demonstrated that, just in a single process, it was feasible to purifying and concentrating dilute nickel wastewater synchronously by the EDI technology...
  89. ncbi Improved purification process for cholera toxin and its application to the quantification of residual toxin in cholera vaccines
    Hyun Jang
    Vaccine Development Section, Laboratory Sciences Division, International Vaccine Institute, Seoul, Korea
    J Microbiol Biotechnol 19:108-12. 2009
    ..The G(M1) ganglioside-binding ELISA was shown to be very sensitive and capable of detecting as little as 1 ng/ml of cholera toxin...
  90. ncbi In-situ graft-polymerization preparation of cation-exchange supermacroporous cryogel with sulfo groups in glass columns
    Kejian Yao
    State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China
    J Chromatogr A 1157:246-51. 2007
    ..Chromatography of lysozyme was carried out to reveal the protein breakthrough and elution characteristics in the obtained cryogel beds...
  91. pmc Comparison between the effect of strongly and weakly cationic exchange resins on matrix physical properties and the controlled release of diphenhydramine hydrochloride from matrices
    Prasert Akkaramongkolporn
    Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand
    AAPS PharmSciTech 11:1104-14. 2010
    ..The results of this study provided useful information on the utilization of ion exchange resins as release modifiers in matrix systems...
  92. ncbi Immobilization of polyethylenimine nanoclusters onto a cation exchange resin through self-crosslinking for selective Cu(II) removal
    Yiliang Chen
    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China
    J Hazard Mater 190:1037-44. 2011
    ..Also, the spent composite adsorbent can be readily regenerated by HCl (0.3M)-NaCl (0.5M) binary solution for repeated use with negligible capacity loss...
  93. ncbi Kinetics of esterification of acidified oil with different alcohols by a cation ion-exchange resin/polyethersulfone hybrid catalytic membrane
    Honglei Zhang
    School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China
    Bioresour Technol 112:28-33. 2012
    ..Furthermore, pseudo-homogeneous kinetic models of the esterification of acidified oil with the four alcohols were established according to the experimental data. The kinetic models can well predict the FFA conversion...
  94. ncbi Methanogenesis in membraneless microbial electrolysis cells
    Peter Clauwaert
    Laboratory of Microbial Ecology and Technology LabMET, Ghent University, Coupure Links 653, 9000, Ghent, Belgium
    Appl Microbiol Biotechnol 82:829-36. 2009
    ....
  95. ncbi Sulfonated and sulfoacylated poly(styrene-divinylbenzene) copolymers as packing materials for cation chromatography
    Andrey V Pirogov
    Chemistry Department, Moscow State University, GPS 2, Lenin Hills, 119992, Moscow, Russia
    Anal Bioanal Chem 376:745-52. 2003
    ..It was found that the separation of alkaline ions and 1,1-dimethylhydrazine (UDMH) were much better that those for the commercial material...
  96. ncbi Confirmatory determination of six penicillins in honey by liquid chromatography/electrospray ionization-tandem mass spectrometry
    Jian Wang
    Canadian Food Inspection Agency, Calgary Laboratory, 3650 36th St, NW, Calgary, T2L 2L1, AB, Canada
    J AOAC Int 87:45-55. 2004
    ..The method limits of confirmation (microg/kg) were 0.44 for amoxicillin, 0.52 for ampicillin, 0.23 for penicillin G, 0.14 for oxacillin, 0.14 for cloxacillin, and 0.15 for dicloxacillin when a sample size of 5 g honey was used...
  97. ncbi Evaluation of analyte stability and method ruggedness in the determination of streptomycin residues in honey by liquid chromatography with post-column derivatization
    Guo Fang Pang
    Qinhuangdao Entry Exit Inspection and Quarantine Bureau, 39 Haibin Rd, Qinhuangdao, Hebei, PC 066002, People s Republic of China
    J AOAC Int 87:39-44. 2004
    ..The continuous 4-year quality analysis also found that C18 solid-phase extraction cartridges should be standardized to ensure that the analytical results are accurate when different lots of cartridges are used...
  98. ncbi Mass influences in the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins
    B Schmidt
    Department of Chemistry, Swiss Federal Institute of Technology Lausanne, Ecublens
    J Chromatogr A 944:149-59. 2002
    ..3 x 10(16) and a steric factor of 224. Using this substance as displacer, a sample mixture containing up to three proteins was separated and the proteins recovered at high yields (80-97%) and in high purity and concentration...
  99. ncbi "Giant diverticulum" sigmoid colon
    Jan Willem R Mulder
    Academic Medical Center of the University of Amsterdam, The Netherlands
    J Am Coll Surg 195:130. 2002
  100. ncbi Retention of alkali, alkaline earth and transition metals on an itaconic acid cation-exchange column. Eluent pH, ionic strength and temperature effects upon selectivity
    Wasim Bashir
    National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Dublin 9, Ireland
    J Chromatogr A 964:113-22. 2002
    ..An investigation into the effect of temperature upon peak efficiency and retention showed increased column temperature could be used to improve the resolution of closely eluting metal ions such as Ca(II) and Sr(II) and Ca(II) and Mn(II)...
  101. ncbi [Study on the defluoridation of drinking water with model-La cation exchange resin]
    Mingyuan Huang
    School of Public Health, Nanhua University, Hengyang 421001, China
    Wei Sheng Yan Jiu 32:553-5. 2003
    ..This method is easy to master and the pH value of the solution doesn't need to be regenerated that the cost of defluoridation can be reduced significantly and it can be used for a long time...

Research Grants5

  1. Synthetic Peptide Consensus Sequence Vaccine Development
    Robert Hodges; Fiscal Year: 2005
    ..abstract_text> ..
  2. LC/MS/MS Purchase
    Robert Hodges; Fiscal Year: 2003
    ..A. Sorkin) Design of peptide analogs/mimetic of the hormones bradykinin and substance P and cloning of the substance P receptor in rat brain. (J. M. Stewart) ..
  3. Analytical Ultracentrifuge Purchase
    Robert Hodges; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  4. Alpbach Workshop:Coiled-Coils, Collagen/Co-proteins
    Robert Hodges; Fiscal Year: 2005
    ..abstract_text> ..
  5. HPLC and CE of Peptides and Proteins
    Robert S Hodges; Fiscal Year: 2010
    ..The development of these methods will have a major impact on proteomics as well. ..