affinity labels

Summary

Summary: Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids.

Top Publications

  1. ncbi The Nano-tag, a streptavidin-binding peptide for the purification and detection of recombinant proteins
    Thorsten Lamla
    Institut fur Biochemie, Freie Universitat Berlin, Thielallee 63, D 14195 Berlin, Germany
    Protein Expr Purif 33:39-47. 2004
  2. ncbi The AviD-tag, a NeutrAvidin/avidin specific peptide affinity tag for the immobilization and purification of recombinant proteins
    Thomas Gaj
    Department of Chemistry, University of Arizona, Tucson, AZ 85721, USA
    Protein Expr Purif 56:54-61. 2007
  3. ncbi Blockade of alcohol's amnestic activity in humans by an alpha5 subtype benzodiazepine receptor inverse agonist
    David J Nutt
    Psychopharmacology Unit, Dorothy Hodgkin Building, University of Bristol, Whitson Street, Bristol, BS1 3NY, UK
    Neuropharmacology 53:810-20. 2007
  4. ncbi Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
    S Chong
    New England Biolabs, Beverly, MA 01915, USA
    Gene 192:271-81. 1997
  5. ncbi An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells
    Tilmann Burckstummer
    Research Center for Molecular Medicine CeMM, Lazarettgasse 19 3, 1090 Vienna, Austria
    Nat Methods 3:1013-9. 2006
  6. pmc Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step
    S Chong
    New England Biolabs Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 26:5109-15. 1998
  7. ncbi A generic protein purification method for protein complex characterization and proteome exploration
    G Rigaut
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Biotechnol 17:1030-2. 1999
  8. ncbi One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag
    A D Keefe
    Department of Molecular Biology, Howard Hughes Medical Institute, Boston, Massachusetts 02114, USA
    Protein Expr Purif 23:440-6. 2001
  9. ncbi Use of fluorescently labeled caspase inhibitors as affinity labels to detect activated caspases
    Jerzy Grabarek
    New York Medical College, Hawthorne, NY 10532, USA
    Hum Cell 15:1-12. 2002
  10. ncbi ATP analogue binding to the A subunit induces conformational changes in the E subunit that involves a disulfide bond formation in plant V-ATPase
    Y Kawamura
    Cryobiosystem Research Center, Iwate University, Iwate, Japan
    Eur J Biochem 268:2801-9. 2001

Detail Information

Publications177 found, 100 shown here

  1. ncbi The Nano-tag, a streptavidin-binding peptide for the purification and detection of recombinant proteins
    Thorsten Lamla
    Institut fur Biochemie, Freie Universitat Berlin, Thielallee 63, D 14195 Berlin, Germany
    Protein Expr Purif 33:39-47. 2004
    ..Additionally, the Nano-tag allowed the detection of recombinant proteins on Western blots by a streptavidin-alkaline phosphatase conjugate...
  2. ncbi The AviD-tag, a NeutrAvidin/avidin specific peptide affinity tag for the immobilization and purification of recombinant proteins
    Thomas Gaj
    Department of Chemistry, University of Arizona, Tucson, AZ 85721, USA
    Protein Expr Purif 56:54-61. 2007
    ..We believe the AviD-tag and its unique recognition properties will provide researchers with a useful new affinity reagent and tool for a variety of applications in the biological and chemical sciences...
  3. ncbi Blockade of alcohol's amnestic activity in humans by an alpha5 subtype benzodiazepine receptor inverse agonist
    David J Nutt
    Psychopharmacology Unit, Dorothy Hodgkin Building, University of Bristol, Whitson Street, Bristol, BS1 3NY, UK
    Neuropharmacology 53:810-20. 2007
    ....
  4. ncbi Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
    S Chong
    New England Biolabs, Beverly, MA 01915, USA
    Gene 192:271-81. 1997
    ..No exogenous proteolytic cleavage is needed. Furthermore, using this procedure, the purified free target protein can be specifically labeled at its C-terminus...
  5. ncbi An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells
    Tilmann Burckstummer
    Research Center for Molecular Medicine CeMM, Lazarettgasse 19 3, 1090 Vienna, Austria
    Nat Methods 3:1013-9. 2006
    ..Using the well-characterized Ku70-Ku80 protein complex as an example, we identified both core elements as well as new candidate effectors...
  6. pmc Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step
    S Chong
    New England Biolabs Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 26:5109-15. 1998
    ..We have constructed general cloning vectors and demonstrated single-column purification of several proteins. In addition, we discuss several factors that may affect the C-terminal peptide bond cleavage activity...
  7. ncbi A generic protein purification method for protein complex characterization and proteome exploration
    G Rigaut
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Biotechnol 17:1030-2. 1999
    ..Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms...
  8. ncbi One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag
    A D Keefe
    Department of Molecular Biology, Howard Hughes Medical Institute, Boston, Massachusetts 02114, USA
    Protein Expr Purif 23:440-6. 2001
    ..We call this method the spin-filter binding inhibition assay...
  9. ncbi Use of fluorescently labeled caspase inhibitors as affinity labels to detect activated caspases
    Jerzy Grabarek
    New York Medical College, Hawthorne, NY 10532, USA
    Hum Cell 15:1-12. 2002
    ..To detect their activation in situ, we applied fluorochrome labeled inhibitors of caspases (FLICA) as affinity labels of active centers of these enzymes...
  10. ncbi ATP analogue binding to the A subunit induces conformational changes in the E subunit that involves a disulfide bond formation in plant V-ATPase
    Y Kawamura
    Cryobiosystem Research Center, Iwate University, Iwate, Japan
    Eur J Biochem 268:2801-9. 2001
    ..This is the first demonstration of interaction between the A and E subunits in the substrate-binding state of a plant V-ATPase...
  11. ncbi Sephadex-based cell-affinity adsorbents: preparation and performance
    Geert Besselink
    Department of Transfusion Technology, CLB, Sanquin Blood Supply Foundation, P O Box 9190, 1066 AC Amsterdam, The Netherlands
    Biotechnol Appl Biochem 35:55-60. 2002
    ..One obvious advantage of the approach described here is that relatively small amounts of SpA and antisera are needed for preparing cell-affinity media...
  12. ncbi Screening of peptide affinity tags using immobilised metal affinity chromatography in 96-well plate format
    Amro Hanora
    Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE 22100 Lund, Sweden
    J Chromatogr A 1087:38-44. 2005
    ..From screening 25 different tags, three clones were able to bind to all metal ions studied (Ni2+, Zn2+, Co2+ and Cd2+). It was clearly demonstrated that the new construct could facilitate the screening of large peptide libraries...
  13. ncbi New role for an old probe: affinity labeling of oxylipid protein conjugates by N'-aminooxymethylcarbonylhydrazino d-biotin
    Juan Chavez
    Department of Chemistry, Oregon State University, Corvallis, Oregon 97331, USA
    Anal Chem 78:6847-54. 2006
    ..The reported approach is outlined for the detection, identification, and characterization of oxylipid peptide conjugates, but the labeling chemistry may also be applicable to other carbonyl-modified proteins...
  14. ncbi Functionally similar vanadate-induced 8-azidoadenosine 5'-[alpha-(32)P]Diphosphate-trapped transition state intermediates of human P-glycoprotin are generated in the absence and presence of ATP hydrolysis
    Z E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-4255, USA
    J Biol Chem 276:21199-208. 2001
    ..alpha-(32)P]8-azido-ADP (or ADP).Vi transition state complexes generated either in the absence of or accompanying [alpha-(32)P]8-azido-ATP hydrolysis are functionally indistinguishable...
  15. ncbi The affinity concept in bioseparation: evolving paradigms and expanding range of applications
    Kalyani Mondal
    Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India
    Biomol Eng 23:59-76. 2006
    ..At industrial level, validation, biosafety and process hygiene are also important aspects. This overview looks at these evolving paradigms and various strategies which utilize affinity phenomenon for protein separations...
  16. ncbi Identification of a contact domain between echistatin and the integrin alpha(v)beta(3) by photoaffinity cross-linking
    L Scheibler
    Division of Bone and Mineral Metabolism, Charles A. Dana and Thorndike Laboratories, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Biochemistry 40:15117-26. 2001
    ..These cross-linking data support the hypothesis that the ligand-bound conformation of the integrin beta(3) subunit differs from the known conformation of I domains...
  17. pmc Use of dual affinity tags for expression and purification of functional peripheral cannabinoid receptor
    Alexei Yeliseev
    Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, 5625 Fishers Lane, Bethesda, MD 20892, USA
    Protein Expr Purif 53:153-63. 2007
    ..Preliminary results of reconstitution experiments indicate that the CB2 has retained its ligand-binding properties...
  18. ncbi Affinity labeling of the rabbit 12/15-lipoxygenase using azido derivatives of arachidonic acid
    Stepan Romanov
    Institute of Biochemistry, University Medicine Berlin Charite, Monbijoustrasse 2, 10117 Berlin, Germany
    Biochemistry 45:3554-62. 2006
    ....
  19. ncbi Nucleotide-binding sites in the functional unit of sarcoplasmic reticulum Ca2+-ATPase as studied by photoaffinity spin-labeled 2-N3-SL-ATP
    T Palm
    Fachbereich Chemie, , Germany
    Biol Chem 382:417-23. 2001
    ..This indicates that the minimal functional unit of the Ca2+-ATPase is a dimer with the nucleotide-binding sites in close proximity...
  20. ncbi DnaA protein Lys-415 is close to the ATP-binding site: ATP-pyridoxal affinity labeling
    T Kubota
    Department of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 288:1141-8. 2001
    ..Thus, this residue is likely close to the bound ATP. Since Lys-415 is located in the DNA-binding domain, these findings imply internal interaction between the domains for ATP binding and DNA binding...
  21. pmc Characterization of the nucleotide-binding capacity and the ATPase activity of the PIP3-binding protein JFC1
    S D Catz
    Department of Molecular and Experimental Medicine, Division of Biochemistry, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 98:11230-5. 2001
    ..Phosphatidylinositol 3,4,5-trisphosphate, a high-affinity ligand of JFC1 did not affect its ATPase kinetics parameters, suggesting that the phosphoinositide have a different role in JFC1 function...
  22. ncbi Proteomic analysis of the binding partners to enteropathogenic Escherichia coli virulence proteins expressed in Saccharomyces cerevisiae
    Philip R Hardwidge
    Veterinary Science Department, South Dakota State University, Brookings, SD, USA
    Proteomics 6:2174-9. 2006
    ..The dataset suggests several potential mammalian targets of these proteins that may guide future experimentation...
  23. ncbi Affinity labeling the dopamine transporter ligand binding site
    Roxanne A Vaughan
    Department of Biochemistry and Molecular Biology, University of North Dakota, School of Medicine and Health Sciences, 501 N Columbia Road, Grand Forks, ND 58203, USA
    J Neurosci Methods 143:33-40. 2005
    ..Using these methods we have identified two distinct regions of DAT that interact with multiple structurally related and diverse irreversible ligands, suggesting that these regions may be involved in the formation of ligand binding sites...
  24. ncbi The First Nucleotide Binding Domain of Cystic Fibrosis Transmembrane Conductance Regulator Is a Site of Stable Nucleotide Interaction, whereas the Second Is a Site of Rapid Turnover
    Luba Aleksandrov
    Mayo Foundation and Mayo Clinic Scottsdale, S C Johnson Medical Research Center, Scottsdale, Arizona 85259, USA
    J Biol Chem 277:15419-25. 2002
    ..This demonstration of NBD2 as the rapid nucleotide turnover site is consistent with the strong effect on channel gating kinetics of inactivation of this domain by mutagenesis...
  25. pmc Efficient identification of photolabelled amino acid residues by combining immunoaffinity purification with MS: revealing the semotiadil-binding site and its relevance to binding sites for myristates in domain III of human serum albumin
    Kohichi Kawahara
    Department of Biofunctional Chemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, 5 1 Ohe Honmachi, Kumamoto 862 0973, Japan
    Biochem J 363:223-32. 2002
    ..These results indicate that FNAK labelling can also be used to monitor Myr binding in domain III. An interpretation for the concomitant local conformational change of HSA is provided...
  26. ncbi Application of isotope coded affinity tag (ICAT) analysis for the identification of differentially expressed proteins following infection of atlantic salmon (Salmo salar) with infectious hematopoietic necrosis virus (IHNV) or Renibacterium salmoninarum (B
    A T Booy
    University of Victoria, Genome British Columbia Proteomics Centre, 3101 4464 Markham Street, Victoria, British Columbia, Canada V8Z 7X8
    J Proteome Res 4:325-34. 2005
    ..Examples of the latter are discussed here and include, a natural killer cell enhancement factor (NKEF), procathepsin L, superoxide-producing NADPH oxidase and interferon-induced viral resistance protein Mx (IFI-Mx)...
  27. ncbi Rapid evaluation of nickel binding properties of His-tagged lactate dehydrogenases using surface plasmon resonance
    Florent Bernaudat
    Department of Pure and Applied Biochemistry, Centre for Chemistry and Chemical Engineering, Lund University, P O Box 124, Getingevagen 60, 221 00 Lund, Sweden
    J Chromatogr A 1066:219-24. 2005
    ..Protein modelling has also proved to be useful in supporting the experimental results...
  28. ncbi A cleavable affinity biotinylating agent reveals a retinoid binding role for RPE65
    Wan Jin Jahng
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 45 Shattuck Street, Boston, Massachusetts 02115, USA
    Biochemistry 42:6159-68. 2003
    ..These studies validate the approach that was used, and furthermore demonstrate that RPE65, a major membrane-associated protein of the RPE, is a RBP...
  29. pmc Analysis of transglutaminase protein substrates by functional proteomics
    Margherita Ruoppolo
    Dipartimento di Biochimica e Biotecnologie Mediche, Universita di Napoli Federico II, Napoli, Italy
    Protein Sci 12:1290-7. 2003
    ..Results obtained in this paper indicate that the whole strategy can be successfully applied in order to identify transglutaminases protein substrates as well as the amino acid site sensitive toward enzyme activity...
  30. ncbi Differential effects of supplementary affinity tags on the solubility of MBP fusion proteins
    Karen M Routzahn
    Protein Engineering Section, Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702, USA
    J Struct Funct Genomics 2:83-92. 2002
    ....
  31. ncbi Functional interaction between the two halves of the photoreceptor-specific ATP binding cassette protein ABCR (ABCA4). Evidence for a non-exchangeable ADP in the first nucleotide binding domain
    Jinhi Ahn
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 278:39600-8. 2003
    ..Our results indicate that only NBD2 of ABCR binds and hydrolyzes ATP in the presence or absence of retinal. NBD1, containing a bound ADP, associates with NBD2 to play a crucial, non-catalytic role in ABCR function...
  32. ncbi Synthesis and screening of a rationally designed combinatorial library of affinity ligands mimicking protein L from Peptostreptococcus magnus
    A Cecília A Roque
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisboa, Portugal
    J Mol Recognit 18:213-24. 2005
    ..The most promising lead, ligand 8/7, when immobilized on an agarose support, behaved in a similar fashion to PpL in isolating Fab fragments from papain digests of human IgG to a final purity of 97%...
  33. ncbi [Tagged-MS method]
    Ryoichi Arai
    Tanpakushitsu Kakusan Koso 49:2763-7. 2004
  34. ncbi Application of protein-coupled liposomes to effective affinity screening from phage library
    Yoichi Kumada
    Graduate School of Science and Technology, Kobe University, Rokkodaicho 1 1, Nada, Kobe, Hyogo 657 8501, Japan
    J Chromatogr A 1080:22-8. 2005
    ..Thus, protein-coupled liposomes are useful as adsorbents for screening from combinatorial phage libraries...
  35. ncbi Immobilisation of a repressor protein for binding of plasmid DNA
    Anja Hasche
    Department of Fermentation Engineering, Faculty of Technology, Bielefeld University, Universitaetsstrasse 25, P O Box 100131, D 33501 Bielefeld, Germany
    J Chromatogr A 1080:76-82. 2005
    ....
  36. ncbi The structure of the mammalian signal recognition particle (SRP) receptor as prototype for the interaction of small GTPases with Longin domains
    Oliver Schlenker
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    J Biol Chem 281:8898-906. 2006
    ..Based on structural conservation we present the SRbeta-GTP:SRX structure as a prototype for conserved interactions in a variety of GTPase regulated targeting events occurring at endomembranes...
  37. ncbi Participation of yeast inosine 5'-monophosphate dehydrogenase in an in vitro complex with a fragment of the C-rich telomeric strand
    Jean Francois Cornuel
    Groupe de Biophysique de l UMR 7643 du CNRS, Ecole Polytechnique, 91128, Palaiseau, France
    Biochimie 84:279-89. 2002
    ..We note that the genes coding for Imd2p and Imd3p are located close to the telomere, and could therefore be subject to silencing by the telomere position effect...
  38. ncbi Investigation of the abundance and subunit composition of GABAA receptor subtypes in the cerebellum of alpha1-subunit-deficient mice
    Waltraud Ogris
    Division of Biochemistry and Molecular Biology, Center for Brain Research, and Section of Biochemical Psychiatry, University Clinic for Psychiatry, Medical University Vienna, Vienna, Austria
    J Neurochem 96:136-47. 2006
    ..Our results do not support a significant compensatory synthesis of other GABAA receptor subunits in the cerebellum of alpha1 -/- mice...
  39. ncbi Element-coded affinity tags for peptides and proteins
    Paul A Whetstone
    Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA
    Bioconjug Chem 15:3-6. 2004
    ..Further, the rare earths are heavy elements, whose mass defects give the masses of tagged peptides exact values not normally shared by molecules that contain only light elements...
  40. ncbi Identification of the bovine gamma-aminobutyric acid type A receptor alpha subunit residues photolabeled by the imidazobenzodiazepine [3H]Ro15-4513
    Gregory W Sawyer
    Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, CA 90095, USA
    J Biol Chem 277:50036-45. 2002
    ..These results are discussed in terms of a homology model of the benzodiazepine-binding site based on the molluscan acetylcholine-binding protein structure...
  41. ncbi The nucleotide-binding site of human sphingosine kinase 1
    Stuart M Pitson
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide SA 5000, Australia
    J Biol Chem 277:49545-53. 2002
    ..It does, however, share some sequence and likely structural similarity with the highly conserved glycine-rich loop, which is known to be involved in anchoring and positioning the nucleotide in the catalytic site of many protein kinases...
  42. ncbi Mapping sites of O-GlcNAc modification using affinity tags for serine and threonine post-translational modifications
    Lance Wells
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21218, USA
    Mol Cell Proteomics 1:791-804. 2002
    ..In addition, our studies emphasize the importance of distinguishing between O-phosphate versus O-GlcNAc when mapping sites of serine and threonine post-translational modification using beta-elimination/Michael addition methods...
  43. ncbi Analysis of phosphoinositide-binding proteins using liposomes as an affinity matrix
    Andreas Knödler
    Oregon Health and Science University, Portland, OR, USA
    Biotechniques 38:858, 860, 862. 2005
  44. ncbi Triatoma infestans apyrases belong to the 5'-nucleotidase family
    Eric Faudry
    Chagas Disease Multidisciplinary Research Laboratory, Department of Pathology, Faculty of Medicine, University of Brasilia, Brazil 70 910 900
    J Biol Chem 279:19607-13. 2004
    ....
  45. ncbi Functional asymmetry of the two nucleotide binding domains in the ABC transporter Ste6
    C Proff
    , , Germany
    Mol Gen Genet 264:883-93. 2001
    ..The experiments further suggest that the two NBDs of Ste6 are not equivalent and affect each other's ability to bind and hydrolyze ATP...
  46. ncbi A single histidine in GABAA receptors is essential for benzodiazepine agonist binding
    H A Wieland
    Laboratory of Molecular Neuroendocrinology, University of Heidelberg, Germany
    J Biol Chem 267:1426-9. 1992
    ..Hence, this histidine present in the alpha 1, alpha 2, alpha 3, and alpha 5 subunits appears to be a key residue for the action of clinically used BZ ligands...
  47. pmc The proteins encoded by the rbs operon of Escherichia coli: II. Use of chimeric protein constructs to isolate and characterize RbsC
    J Zaitseva
    Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907 1153, USA
    Protein Sci 5:1100-7. 1996
    ..This is a substantial increase in amounts from any previous RbsC production vectors. All proteins from the rbs operon have now been overproduced and substantially purified...
  48. ncbi Characterization of KpsT, the ATP-binding component of the ABC-transporter involved with the export of capsular polysialic acid in Escherichia coli K1
    M S Pavelka
    Department of Microbiology and Immunology, University of Rochester Medical Center, New York 14642
    J Biol Chem 269:20149-58. 1994
    ..The results obtained from chemical mutagenesis of kpsT are consistent with the model and revealed characteristics particular to capsule transporters...
  49. ncbi Spermidine-preferential uptake system in Escherichia coli. ATP hydrolysis by PotA protein and its association with membrane
    K Kashiwagi
    Faculty of Pharmaceutical Sciences, Chiba University, Japan
    J Biol Chem 270:25377-82. 1995
    ..ATPase of PotA protein was inhibited by spermidine, suggesting that uptake inhibition by spermidine may function during this process...
  50. ncbi Identification of the ubiquinone-binding domain in QPs1 of succinate-ubiquinone reductase
    G Y Lee
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078
    J Biol Chem 270:6193-8. 1995
    ..The Q-binding domain, using the proposed structure of QPs1, is probably located in the stretch connecting transmembrane helices 2 and 3 that extrude from the surface of the M side of the inner membrane...
  51. ncbi Photoaffinity labeling of human placental S-adenosylhomocysteine hydrolase with [2-3H]8-azido-adenosine
    C S Yuan
    Department of Biochemistry, University of Kansas, Lawrence 66045, USA
    J Biol Chem 270:16140-6. 1995
    ..The peptide Val319-Arg327 is adjacent to Leu330, which is proposed by a computer graphics model to interact with the C-6-NH2 group of Ado...
  52. ncbi Catalytic and noncatalytic nucleotide binding sites of the Escherichia coli F1 ATPase. Amino acid sequences of beta-subunit tryptic peptides labeled with 2-azido-ATP
    J G Wise
    Department of Chemistry and Biochemistry, University of California, Los Angeles 90024 1570
    FEBS Lett 223:395-401. 1987
    ..These beta-subunit peptides were labeled on tyrosine-331 (catalytic sites) and tyrosine-354 (noncatalytic sites) in homology with the labeling patterns of the mitochondrial and chloroplast enzymes...
  53. ncbi Photoaffinity labeling of terminal deoxynucleotidyl transferase. 2. Identification of peptides in the nucleotide binding domain
    R K Evans
    Department of Biochemistry, University of Kentucky, Lexington 40536 0084
    Biochemistry 28:713-20. 1989
    ..Structure predictions, based on sequence data, place the two peptides identified by photolabeling in spatial proximity consistent with the participation of both in the nucleotide binding domain...
  54. ncbi The nucleotide-binding site of HisP, a membrane protein of the histidine permease. Identification of amino acid residues photoaffinity labeled by 8-azido-ATP
    C S Mimura
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    J Biol Chem 265:19535-42. 1990
    ..These consensus motifs are found in many purine nucleotide-binding proteins. The relationship between the location of these residues and the overall structure of the ATP-binding site is discussed...
  55. ncbi Identification of proteins binding to interferon-inducible transcriptional enhancers in hematopoietic cells
    A Wedrychowski
    Department of Hematology, University of Texas M D Anderson Cancer Center, Houston 77030
    J Biol Chem 267:4533-40. 1992
    ..Following exposure to alpha-interferon, more slowly migrating complexes appeared which contained a 48-kDa protein, a 95-kDa protein, and a 105-kDa protein which bound to the 9-27 transcriptional enhancer in a sequence-specific manner...
  56. ncbi Distribution of GLUT3 glucose transporter protein in human tissues
    P R Shepherd
    Charles A Dana Research Institute, Beth Israel Hospital, Boston, MA 02215
    Biochem Biophys Res Commun 188:149-54. 1992
    ..No GLUT3 was detected in membranes from any of 3 skeletal muscle groups investigated. We conclude that a major role of GLUT3 in humans is as the brain neuronal glucose transporter...
  57. ncbi The first phospholipase inhibitor from the serum of Vipera ammodytes ammodytes
    Jernej Sribar
    Department of Molecular and Biomedical Sciences, Jozef Stefan Institute, Ljubljana, Slovenia
    FEBS J 274:6055-64. 2007
    ....
  58. ncbi Selection of optimum affinity tags from a phage-displayed peptide library. Application to immobilized copper(II) affinity chromatography
    A V Patwardhan
    Department of Chemical Engineering, University of Pittsburgh, PA 15219, USA
    J Chromatogr A 787:91-100. 1997
    ....
  59. ncbi Improved anti-IgG and HSA affinity ligands: clinical application of VHH antibody technology
    Rinse Klooster
    Department of Cellular Architecture and Dynamics, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Immunol Methods 324:1-12. 2007
    ..Moreover, as VHHs are single protein chains, they can be coupled relatively easily to solid matrices. These three factors are important for developing affinity purification medication...
  60. ncbi Histidines in affinity tags and surface clusters for immobilized metal-ion affinity chromatography of trimeric tumor necrosis factor alpha
    V Gaberc-Porekar
    National Institute of Chemistry, Hajdrihova, Ljubljana, Slovenia
    J Chromatogr A 852:117-28. 1999
    ..Histidines, relatively rigidly inserted in the structure, as in our model proteins, display superior chromatographic characteristics vis a vis flexible tags with the same total number of histidines...
  61. pmc Analysis of a nucleotide-binding site of 5-lipoxygenase by affinity labelling: binding characteristics and amino acid sequences
    Y Y Zhang
    Whitaker Cardiovascular Institute, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
    Biochem J 351:697-707. 2000
    ..Given the stoichiometry of the labelling, the two peptide sequences of 5LO were probably near each other in the enzyme's tertiary structure, composing or surrounding the ATP-binding site of 5LO...
  62. ncbi Natural poly-histidine affinity tag for purification of recombinant proteins on cobalt(II)-carboxymethylaspartate crosslinked agarose
    G Chaga
    CLONTECH Laboratories, Inc, Palo Alto, CA 94303, USA
    J Chromatogr A 864:247-56. 1999
    ..Recovery for each purified protein was higher than 77% of the initial loaded amount as judged by biological activity. The operational capacity of Co2+-carboxymethylaspartate agarose for each protein was determined...
  63. ncbi Novel mode of ligand binding by the SH2 domain of the human XLP disease gene product SAP/SH2D1A
    S C Li
    Program in Molecular Biology and Cancer, Department of Molecular and Medical Genetics, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, Canada
    Curr Biol 9:1355-62. 1999
    ..The SAP-SLAM interaction can occur in a phosphorylation-independent manner...
  64. ncbi Photolabeling of the phosphate binding site of chloroplast coupling factor 1 with [32P]azidonitrophenyl phosphate
    L Michel
    Laboratoire de Biochimie URA 1130 CNRS, Departement de Biologie Moleculaire et Structurale, Centre d Etudes Nucléaires, Grenoble, France
    FEBS Lett 313:90-3. 1992
    ..The labeled beta-Tyr328 of CF1 is the equivalent of beta-Tyr311 of F1 from beef heart mitochondria, which was previously found to be photolabeled by ANPP [J. Garin et al. (1989) Biochemistry 28, 1442-1448]...
  65. ncbi The quinone-binding site in succinate-ubiquinone reductase from Escherichia coli. Quinone-binding domain and amino acid residues involved in quinone binding
    X Yang
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 273:31916-23. 1998
    ..The hydroxyl group, but not the size of the amino acid side chain, at position 33 of SdhC is also important, because Ser-33 can be substituted with threonine but not with alanine...
  66. pmc Variability in the immunodetection of His-tagged recombinant proteins
    Natasa Debeljak
    Laboratory for Cell and Molecular Biology, Division of Hematology and Oncology, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, 330 Brookline Ave, W BL 548, Boston, MA 02215, USA
    Anal Biochem 359:216-23. 2006
    ..Such variability in His-tag immunorecognition can lead to critical adverse effects on several analytical methods...
  67. ncbi Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging
    Daniela C Dieterich
    Division of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    Nat Protoc 2:532-40. 2007
    ..This protocol can be completed in 5 days...
  68. pmc Neuropharmacology of the naturally occurring kappa-opioid hallucinogen salvinorin A
    Christopher W Cunningham
    Department of Medicinal Chemistry, University of Kansas, Lawrence, KS 66045 7582, USA
    Pharmacol Rev 63:316-47. 2011
    ..salvinorin A responsible for opioid receptor affinity and selectivity has produced numerous receptor probes, affinity labels, and tools for evaluating the biological processes responsible for its observed psychological effects...
  69. ncbi The molecular basis for the mode of action of bicyclomycin
    Harold Kohn
    Division of Medicinal Chemistry and Natural Products, School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7360, USA
    Curr Drug Targets Infect Disord 5:273-95. 2005
    ..obtained from bicyclomycin structure-activity studies, site-directed mutagenesis investigations, bicyclomycin affinity labels, and biochemical and biophysical measurements with recent X-ray crystallographic images of the bicyclomycin-..
  70. ncbi Affinity labeling of oxaloacetate decarboxylase by novel dichlorotriazine linked alpha-ketoacids
    N E Labrou
    Department of Agricultural Biotechnology, Agricultural University of Athens, Greece
    J Protein Chem 18:729-33. 1999
    ..and beta-mercaptopyruvic acid linked to the reactive diclorotriazine ring, were studied as active site-direct affinity labels towards oxaloacetate decarboxylase (EC 4.1.1.3, OXAD)...
  71. pmc 2-Dialkynyl derivatives of (N)-methanocarba nucleosides: 'Clickable' A(3) adenosine receptor-selective agonists
    Dilip K Tosh
    Molecular Recognition Section, Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bldg 8A, Rm B1A 19, NIH, NIDDK, LBC, Bethesda, MD 20892, United States
    Bioorg Med Chem 18:508-17. 2010
    ..Other potent, selective derivatives (K(i) at A(3)AR innM) were intended as possible receptor affinity labels: 3-nitro-4-fluorophenyl (10.6), alpha-bromophenacyl (9...
  72. pmc Cutting edge: FcR-like 5 on innate B cells is targeted by a poxvirus MHC class I-like immunoevasin
    Jessica A Campbell
    Division of Pediatric Rheumatology, Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Immunol 185:28-32. 2010
    ..Due to their binding specificities, immunoevasins can be exploited as affinity labels to identify host-encoded molecules of previously unsuspected importance in defense against the relevant class ..
  73. ncbi Cysteine proteinase inhibitors as therapy for parasitic diseases: advances in inhibitor design
    Dietmar Steverding
    Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich NR4 7TJ, UK
    Mini Rev Med Chem 6:1025-32. 2006
    ..Also, the development of activity-based affinity labels has enabled the identification and characterization of potential cysteine proteinase targets in situ...
  74. ncbi Affinity labels as tools for the identification of opioid receptor recognition sites
    P S Portoghese
    Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA
    Farmaco 56:191-6. 2001
    b>Affinity labels have proven to be useful tools in opioid research...
  75. pmc Discovery of halopyridines as quiescent affinity labels: inactivation of dimethylarginine dimethylaminohydrolase
    Corey M Johnson
    Division of Medicinal Chemistry, College of Pharmacy, University of Texas, Austin, Texas 78712, United States
    J Am Chem Soc 133:1553-62. 2011
    ..To our knowledge, halopyridines have not previously been reported as protein modifiers, and therefore they represent a first-in-class example of a novel type of quiescent affinity label...
  76. ncbi Immunoassays based on electrochemical detection using microelectrode arrays
    K Dill
    CombiMatrix Corporation, Harbour Pointe Tech Center, 6500 Harbour Heights Parkway, Suite 301, Mukilteo, WA 98275, USA
    Biosens Bioelectron 20:736-42. 2004
    ..Antibodies are tagged with coded affinity labels and then allowed to self-assemble on the appropriate electrode assay sites...
  77. ncbi Selective chemical labeling of proteins in living cells
    Lawrence W Miller
    Department of Chemistry, Columbia University, Havemeyer Hall, MC 3153, 3000 Broadway, New York, New York 10027, USA
    Curr Opin Chem Biol 9:56-61. 2005
    Labeling proteins with fluorophores, affinity labels or other chemically or optically active species is immensely useful for studying protein function in living cells or tissue...
  78. ncbi Analogs of Manduca adipokinetic hormone tested in a bioassay and in a receptor-binding assay
    R Ziegler
    Department of Biochemistry and Center for Insect Science, University of Arizona, Tucson 85721, USA
    Peptides 19:481-6. 1998
    ..In addition an extended M-AKH and analogs containing photo affinity labels were tested. All analogs had reduced activity...
  79. doi The thyroid hormone transporters MCT8 and MCT10 transport the affinity-label N-bromoacetyl-[(125)I]T3 but are not modified by it
    W Edward Visser
    Department of Internal Medicine, Erasmus University Medical Center, Dr Molewaterplein, Rotterdam, The Netherlands
    Mol Cell Endocrinol 337:96-100. 2011
    ..The usefulness of BrAc[(125)I]T3 as a tool for the identification of novel TH transporters remains to be explored...
  80. ncbi Synthesis and spectroscopic characterization of photo-affinity peptide ligands to study rhodopsin-G protein interaction
    Yihui Chen
    Department of Chemistry, Columbia University, New York, NY, USA
    Photochem Photobiol 84:831-8. 2008
    ..Here, we describe novel peptides with photo-affinity labels that bind R* with affinities similar to that of the native G(t)gamma(60-71)far peptide...
  81. pmc Disparate proteome reactivity profiles of carbon electrophiles
    Eranthie Weerapana
    The Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Chem Biol 4:405-7. 2008
    ..reactivity of electrophiles are crucial for designing activity-based probes for enzymes lacking cognate affinity labels. Here, we show that different classes of carbon electrophiles exhibit markedly distinct amino acid labeling ..
  82. doi Protein-inorganic array construction: design and synthesis of the building blocks
    Niculina D Bogdan
    Department of Chemistry, Marie Curie Excellence Team, Technical University Munchen, 4 Lichtenberg Str 85748 Garching, Germany
    Chemistry 16:2170-80. 2010
    ..the way to the synthesis of di-functional ligands containing more elaborated polypyridine ligands as well as affinity labels for different enzyme families...
  83. ncbi The mitochondrial permeability transition pore
    Paolo Bernardi
    Department of Biomedical Sciences and CNR Institute of Neurosciences, University of Padova, Viale Giuseppe Colombo 3, I 35121 Padova, Italy
    Novartis Found Symp 287:157-64; discussion 164-9. 2007
    ..While the nature of the components forming the PTP remains controversial, the identification of novel inhibitors that can be used as affinity labels is offering new perspectives towards the molecular definition of the PTP.
  84. ncbi An efficient, one-pot synthesis of various ceramide 1-phosphates from sphingosine 1-phosphate
    Peter Nussbaumer
    Novartis Institutes for BioMedical Research, Brunner Strasse 59, A 1235 Vienna, Austria
    Chem Phys Lipids 151:125-8. 2008
    ..Thus, ceramide 1-phosphates with various fatty acid chains and with fluorescent and affinity labels attached to the sphingoid backbone were prepared in good yields.
  85. ncbi Functionalized congeners of tyrosine-based P2X(7) receptor antagonists: probing multiple sites for linking and dimerization
    Wangzhong Chen
    Molecular Recognition Section, Laboratory of Bioorganic Chemistry, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0810, USA
    Bioconjug Chem 13:1100-11. 2002
    ..acetylacetonate to amines, which were either converted to the isothiocyanate groups, as potential affinity labels for the receptor, or acylated, as models for conjugation...
  86. pmc Global landscape of HIV-human protein complexes
    Stefanie Jäger
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94158, USA
    Nature 481:365-70. 2012
    ..This data set facilitates a more comprehensive and detailed understanding of how the host machinery is manipulated during the course of HIV infection...
  87. doi Affinity proteomics: the role of specific binding reagents in human proteome analysis
    Oda Stoevesandt
    Protein Technology Group, Babraham Bioscience Technologies Ltd, Babraham Research Campus, Cambridge, UK
    Expert Rev Proteomics 9:401-14. 2012
    ..In addition to current affinity proteomics methods and applications, including arrays of proteins, binders, lysates and tissues, approaches coupling mass spectrometry-based proteomics and affinity proteomics are reviewed...
  88. doi Engineering of affibody molecules for therapy and diagnostics
    Joachim Feldwisch
    Affibody AB, Solna, Sweden
    Methods Mol Biol 899:103-26. 2012
    ..Obviously, the optimal molecule for molecular imaging will be different from the optimal molecule for therapy. Here, we describe general strategies to optimize Affibody molecules for diagnostic imaging and therapy applications...
  89. pmc Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tags
    Alix Salvail-Lacoste
    Departement de Biochimie, Universite de Montreal, C P 6128, Succursale Centre Ville, Montreal, QC H3C 3J7, Canada
    RNA 19:1003-14. 2013
    ..Moreover, the 5'-CRISPR-based strategy can be combined with affinity purification using the 3'-ARiBo tag for quick purification of RNA with both 5' and 3' homogeneity...
  90. ncbi Probing the nucleotide binding sites of axonemal dynein with the fluorescent nucleotide analogue 2'(3')-O-(-N-Methylanthraniloyl)-adenosine 5'-triphosphate
    G Mocz
    Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu 96822, USA
    Biochemistry 37:9862-9. 1998
    ..The distinct affinities of the individual binding sites are consistent with a physiological role in regulating nucleotide binding...
  91. ncbi Proteomics in medicinal chemistry
    Satomi Niwayama
    Department of Chemistry and Biochemistry, Texas Tech University, Box 41061, Lubbock, 79409 1061, USA
    Mini Rev Med Chem 6:241-6. 2006
    ..An important aspect of proteomics is to identify and quantify proteins. Many new technologies and techniques have been developed in this field and have been applied to various aspects of drug discovery...
  92. ncbi Affinity-tagged green fluorescent protein (GFP) extraction from a clarified E. coli cell lysate using a two-phase aqueous micellar system
    Priscila G Mazzola
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biotechnol Bioeng 93:998-1004. 2006
    ....
  93. ncbi Isolation of cell specific peptide ligands using fluorescent bacterial display libraries
    Karen Y Dane
    Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA
    J Immunol Methods 309:120-9. 2006
    ..Fluorescent display libraries thus provide a powerful new methodology for parallel identification of cell specific affinity ligands...
  94. ncbi Cloning of the human equilibrative, nitrobenzylmercaptopurine riboside (NBMPR)-insensitive nucleoside transporter ei by functional expression in a transport-deficient cell line
    C R Crawford
    Department of Molecular Pharmacology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Biol Chem 273:5288-93. 1998
    ..These data suggest that the delayed early response gene HNP36 is a truncated form of ENT2 and that the full-length open reading frame of ENT2 is required for production of a functional plasma membrane ei transporter...
  95. ncbi Evaluation of homologous, heterologous, and affinity conjugates for the serodiagnosis of Toxoplasma gondii and Neospora caninum in maned wolves (Chrysocyon brachyurus)
    D A O Silva
    Laboratory of Immunoparasitology, Universidade Federal de Uberlandia, MG, Brasil
    J Parasitol 91:1212-6. 2005
    ..gondii in wild canids, whereas for N. caninum infection, only the homologous or heterologous fluorescent conjugates have been shown to be useful...
  96. ncbi Sequential ATP hydrolysis by Cdc6 and ORC directs loading of the Mcm2-7 helicase
    John C W Randell
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Mol Cell 21:29-39. 2006
    ..Importantly, these coordinated yet distinct functions of ORC and Cdc6 ensure the correct temporal and spatial regulation of pre-RC formation...
  97. ncbi Photoaffinity labelling of a DNA-binding site on the globular domain of histone H5
    F A Goytisolo
    Cambridge Centre for Molecular Recognition, University of Cambridge, UK
    Eur J Biochem 242:619-26. 1996
    ..1994) Curr. Opin. Struct. Biol. 4. 44-50]...
  98. ncbi A dual affinity tag on the 64-kDa Nlt1p subunit allows the rapid characterization of mutant yeast oligosaccharyl transferase complexes
    R Pathak
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125, USA
    Arch Biochem Biophys 338:1-6. 1997
    ..Yeast strains bearing affinity-tagged enzyme subunits allow the rapid characterization of native and mutant transferase complexes...
  99. ncbi Proteomics for cancer biomarker discovery
    Pothur R Srinivas
    Division of Cancer Prevention, National Cancer Institute, Rockville, MD 20852, USA
    Clin Chem 48:1160-9. 2002
    ..Finally, the review touches on the efforts of the Early Detection Research Network at the National Cancer Institute in promoting biomarker discovery for translation at the clinical level...
  100. ncbi A highly decreased binding of cyclic adenosine monophosphate to protein kinase A in erythrocyte membranes is specific for active psoriasis
    Rudolf E Schopf
    Department of Dermatology, Johannes Gutenberg University, Langenbeckstrasse 1, 55101 Mainz, Germany
    J Invest Dermatol 119:160-5. 2002
    ..It was concluded that the decreased binding of cyclic adenosine monophosphate to protein kinase A in erythrocytes is specific for psoriasis and normalizes after successful treatment...
  101. ncbi Photoaffinity labeling of human sex hormone-binding globulin using 17alpha-alkylamine derivatives of 3beta-androstanediol substituted with azidonitrophenylamido, azidonitrophenylamino, or trifluoroazidonitrophenylamino chromophores. Localization of Trp-84
    C Chambon
    , , , , 69322 Lyon, France
    Biochemistry 40:15424-35. 2001
    ....

Research Grants69

  1. SKIN REACTION TO LIGHT--NORMAL, PHOTOSENSITIVITY, CANCER
    MADHUKAR PATHAK; Fiscal Year: 1980
    ..A new technique of photoaffinity labeling of enzymes using phtoreactive affinity labels mimicking folic acid is proposed...
  2. NEW CHEMICAL APPROACHES TO ADP-RIBOSYL TRANSFER
    James Slama; Fiscal Year: 1990
    ADP-ribosyl transferases are an important class of regulatory enzymes. This project proposes the design and affinity labels specific for the NAD substrate binding site of ADP-ribosyl transferases...
  3. NUCLEOSIDE, NUCLEOTIDE, AND POLYNUCLEOTIDE ANALOGS
    Donald Bergstrom; Fiscal Year: 1980
    ..Once achieved, methods for transforming the amino function to useful affinity labels and reporter groups will be investigated...
  4. PORPHOBILINOGEN SYNTHASE PROBES OF THE ACTIVE SITE
    Eileen Jaffe; Fiscal Year: 1990
    ..addition products and characterize their behavior as alternative substrates, reversible inhibitors, or affinity labels of PBGS...
  5. PORPHOBILINOGEN SYNTHASE, PROBES OF THE ACTIVE SITE
    Eileen Jaffe; Fiscal Year: 1992
    ..addition products and characterize their behavior as alternative substrates, reversible inhibitors, or affinity labels of PBGS...
  6. AFFINITY LABELS FOR INOSITOL POLYPHOSPHATE RECEPTORS
    Glenn Prestwich; Fiscal Year: 1992
    ..Chemical affinity labels, photoaffinity labels, and immobilized affinity matrices for proteins which recognize IP3, IP4, and IP6 will ..
  7. PORPHOBILINOGEN SYNTHASE, PROBES OF THE ACTIVE SITE
    Eileen Jaffe; Fiscal Year: 1993
    ..addition products and characterize their behavior as alternative substrates, reversible inhibitors, or affinity labels of PBGS...
  8. CHARACTERIZATION OF RAT KIDNEY GLUTAMINASE ISOENZYMES
    Norman Curthoys; Fiscal Year: 1980
    ..The stoichiometry and kinetics of inactivation of the glutaminase with either of two affinity labels, 6-diazo-5-oxo-l-norleucine and L-2-amino-4-oxo-5-chloropentanoic acid, has been characterized...
  9. AFFINITY LABELS FOR INOSITOL POLYPHOSPHATE RECEPTORS
    Glenn Prestwich; Fiscal Year: 1999
    ..The affinity reagents and the binding domain data obtained from these reagents will be important in defining the mechanisms of these ubiquitous and varied molecular switches. ..
  10. Structural basis of specificity in affinity-labeled polyketide synthase didomains
    Janet Smith; Fiscal Year: 2009
    ..b>Affinity labels have been demonstrated to be an effective means to establish the mechanism of pikromycin thioesterase (Pik TE)..
  11. Structural basis of specificity in affinity-labeled polyketide synthase didomains
    Janet L Smith; Fiscal Year: 2011
    ..b>Affinity labels have been demonstrated to be an effective means to establish the mechanism of pikromycin thioesterase (Pik TE)..
  12. Structural basis of specificity in affinity-labeled polyketide synthase didomains
    Janet L Smith; Fiscal Year: 2010
    ..b>Affinity labels have been demonstrated to be an effective means to establish the mechanism of pikromycin thioesterase (Pik TE)..
  13. PCP RECEPTOR CHARACTERIZATION WITH NEW AFFINITY LIGANDS
    Eckard Weber; Fiscal Year: 1990
    ..Computer modelling will be utilized to develop yet other novel PCP receptor (affinity) ligands. Proposed affinity labels for the NMDA binding site are based on the antagonist CPP...
  14. PCP RECEPTOR CHARACTERIZATION WITH NEW AFFINITY LIGANDS
    Eckard Weber; Fiscal Year: 1992
    ..Computer modelling will be utilized to develop yet other novel PCP receptor (affinity) ligands. Proposed affinity labels for the NMDA binding site are based on the antagonist CPP...
  15. CHALCONE ISOMERASE--MECHANISM OF CATALYSIS
    RODNEY BEDNAR; Fiscal Year: 1990
    ..b>Affinity labels will be used to identify a putative enzymic base...
  16. ANALYSIS OF GIARDIA PYROPHOSPHATE DEPENDENT KINASES
    NELSON PHILLIPS; Fiscal Year: 1991
    ..These affinity labels will be used as probes to isolate tryptic peptides at or around the PPi binding and catalytic sites by ..
  17. SIGNAL TRANSDUCTION OF A G PROTEIN COUPLED RECEPTOR
    Athan Kuliopulos; Fiscal Year: 1999
    ..binding site will be done with a combination of N-bromoacetyl- and benzoylphenylalanine-alphaFactor peptide affinity labels. N-terminally truncated alphaFactor peptide affinity labels are antagonists and will serve as probes of the ..
  18. ACTIVE SITES OF CREATINE KINASE FROM HEART AND MUSCLE
    GEORGE KENYON; Fiscal Year: 1991
    ..Such analogs may be novel substrates, competitive inhibitors or affinity labels. Various physical and biochemical methods, including kinetic studies, nuclear magnetic resonance studies, ..
  19. BIOCHEMICAL CHARACTERIZATION OF OPIOID BINDING SITES
    Gavril Pasternak; Fiscal Year: 1993
    ..Finally, we will extend our studies utilizing affinity labels synthesized in our laboratory to investigate binding heterogeneity at the molecular level...
  20. STRUCTURAL STUDIES ON THE ENERGY TRANSDUCING ATPASE
    William Allison; Fiscal Year: 1980
    ..Analogues of ATP that are potential affinity labels for the enzyme will be synthesized for these studies...
  21. BIOCHEMICAL CHARACTERIZATION OF OPIOID BINDING SITES
    Gavril Pasternak; Fiscal Year: 1992
    ..Finally, we will extend our studies utilizing affinity labels synthesized in our laboratory to investigate binding heterogeneity at the molecular level...
  22. POTENTIAL ANTICARIES AGENTS
    RONALD DOYLE; Fiscal Year: 1980
    ..The plan is to develop site-directed irreversible enzyme inhibitors (affinity labels) for Streptococcus mutans glucosyltransferases, key bacterial enzymes implicated in the initiation and ..
  23. BETA-ADRENERGIC ACTIVATION OF A HEART CHLORIDE CHANNEL
    Kenneth Walsh; Fiscal Year: 1992
    ..long-term goal of locating the sites of phosphorylation and ligand binding on the protein and developing high affinity labels for the channel...
  24. IMIDAZOLINES AS PROBES OF IGRS AND ALPHA-ADRENOCEPTORS
    Duane Miller; Fiscal Year: 1993
    ..imidazolines IBI analogs; characterization of the molecular mechanism of action of IBI and imidazoline affinity labels on IGRS in selected tissues (aorta, brain, platelets and adipocytes); investigation of the competitive ..
  25. Sigma Receptor Structure/Function/K+ Channel Modulation
    ARNOLD RUOHO; Fiscal Year: 2005
    ..1) synthesis and characterization of novel high affinity sigma receptor agonist and antagonist photo affinity labels; (2) mapping the ligand binding site(s) of the sigma1 receptor; and (3) determination of the properties of ..
  26. EPOTHILONE AFFINITY LABELS
    Gunda Georg; Fiscal Year: 2001
    ..It is the plan to prepare epothilone affinity labels through emisynthesis and total synthesis and evaluate them for tubulin assembly activity and cytotoxicity...
  27. EPOTHILONE AFFINITY LABELS
    Gunda Georg; Fiscal Year: 2000
    ..It is the plan to prepare epothilone affinity labels through emisynthesis and total synthesis and evaluate them for tubulin assembly activity and cytotoxicity...
  28. EPOTHILONE AFFINITY LABELS
    Gunda Georg; Fiscal Year: 2001
    ..It is the plan to prepare epothilone affinity labels through emisynthesis and total synthesis and evaluate them for tubulin assembly activity and cytotoxicity...
  29. EPOTHILONE AFFINITY LABELS
    Gunda Georg; Fiscal Year: 2002
    ..It is the plan to prepare epothilone affinity labels through emisynthesis and total synthesis and evaluate them for tubulin assembly activity and cytotoxicity...
  30. EPOTHILONE AFFINITY LABELS
    Gunda Georg; Fiscal Year: 1999
    ..It is the plan to prepare epothilone affinity labels through emisynthesis and total synthesis and evaluate them for tubulin assembly activity and cytotoxicity...
  31. Sigma Receptor Structure /Function /K+ Channel Modulation
    ARNOLD RUOHO; Fiscal Year: 2007
    ..1) synthesis and characterization of novel high affinity sigma receptor agonist and antagonist photo affinity labels; (2) mapping the ligand binding site(s) of the sigma1 receptor; and (3) determination of the properties of ..
  32. EPOTHILONE AFFINITY LABELS
    Gunda Georg; Fiscal Year: 2002
    ..It is the plan to prepare epothilone affinity labels through emisynthesis and total synthesis and evaluate them for tubulin assembly activity and cytotoxicity...
  33. EPOTHILONE AFFINITY LABELS
    Gunda Georg; Fiscal Year: 2003
    ..It is the plan to prepare epothilone affinity labels through emisynthesis and total synthesis and evaluate them for tubulin assembly activity and cytotoxicity...
  34. Sigma Receptor Structure/Function/K+ Channel Modulation
    ARNOLD RUOHO; Fiscal Year: 2004
    ..1) synthesis and characterization of novel high affinity sigma receptor agonist and antagonist photo affinity labels; (2) mapping the ligand binding site(s) of the sigma1 receptor; and (3) determination of the properties of ..
  35. EPOTHILONE AFFINITY LABELS
    Gunda Georg; Fiscal Year: 2003
    ..It is the plan to prepare epothilone affinity labels through emisynthesis and total synthesis and evaluate them for tubulin assembly activity and cytotoxicity...
  36. Sigma Receptor Structure /Function/K+ Channel Modulation
    ARNOLD RUOHO; Fiscal Year: 2003
    ..1) synthesis and characterization of novel high affinity sigma receptor agonist and antagonist photo affinity labels; (2) mapping the ligand binding site(s) of the sigma1 receptor; and (3) determination of the properties of ..
  37. Sigma Receptor Structure/Function/K+ Channel Modulation
    ARNOLD RUOHO; Fiscal Year: 2006
    ..1) synthesis and characterization of novel high affinity sigma receptor agonist and antagonist photo affinity labels; (2) mapping the ligand binding site(s) of the sigma1 receptor; and (3) determination of the properties of ..
  38. EPOTHILONE AFFINITY LABELS
    Gunda Georg; Fiscal Year: 2000
    ..It is the plan to prepare epothilone affinity labels through emisynthesis and total synthesis and evaluate them for tubulin assembly activity and cytotoxicity...
  39. Liquid Chormatograph Mass Spectrometer (LC-MS)
    MURRAY JOHNSTON; Fiscal Year: 2002
    ..These projects include: (Colman) characterization of new affinity labels, chemically modified enzymes and purified modified peptides and mutant enzymes; (Koh) selective regulation of ..
  40. STRUCTURE AND FUNCTION OF STEROID-BINDING PROTEINS
    Robert Kuhn; Fiscal Year: 1980
    The objectives of this study are to study the active site of steroid binding proteins using affinity labels, develop an RIA for progesterone binding globulin, determine the site of and factors controlling the synthesis of PBG, study the ..
  41. PEPTIDE RECEPTORS IN BIOLOGICAL MEMBRANES
    JAMES STAROS; Fiscal Year: 1990
    ..structure of the active site of the kinase domain will be investigated through the use of a set of ATP-analog affinity labels which are expected to label different parts of the ATP binding site...
  42. SUSTRATE SPECIFICITY OF NONRECEPTOR TYROSINE KINASE
    W Miller; Fiscal Year: 2001
    ..substrate analogs for these enzymes containing the photoactive amino acid p-benzoyl-Phe will be used as the affinity labels. Modified regions inside and outside the SH2 and SH3 domains will be studied further by site-directed ..
  43. SUSTRATE SPECIFICITY OF NONRECEPTOR TYROSINE KINASE
    W Miller; Fiscal Year: 1999
    ..substrate analogs for these enzymes containing the photoactive amino acid p-benzoyl-Phe will be used as the affinity labels. Modified regions inside and outside the SH2 and SH3 domains will be studied further by site-directed ..
  44. SUSTRATE SPECIFICITY OF NONRECEPTOR TYROSINE KINASE
    W Miller; Fiscal Year: 2002
    ..substrate analogs for these enzymes containing the photoactive amino acid p-benzoyl-Phe will be used as the affinity labels. Modified regions inside and outside the SH2 and SH3 domains will be studied further by site-directed ..
  45. SUBSTRATE SPECIFICITY OF NONRECEPTOR TYROSINE KINASES
    W Miller; Fiscal Year: 1993
    ..substrate analogs for these enzymes containing the photoactive amino acid p-benzoyl-Phe will be used as the affinity labels. Modified regions inside and outside the SH2 and SH3 domains will be studied further by site-directed ..
  46. SUSTRATE SPECIFICITY OF NONRECEPTOR TYROSINE KINASE
    W Miller; Fiscal Year: 2000
    ..substrate analogs for these enzymes containing the photoactive amino acid p-benzoyl-Phe will be used as the affinity labels. Modified regions inside and outside the SH2 and SH3 domains will be studied further by site-directed ..
  47. SUSTRATE SPECIFICITY OF NONRECEPTOR TYROSINE KINASE
    W Miller; Fiscal Year: 2003
    ..substrate analogs for these enzymes containing the photoactive amino acid p-benzoyl-Phe will be used as the affinity labels. Modified regions inside and outside the SH2 and SH3 domains will be studied further by site-directed ..
  48. MECHANISM OF GLUCOSE TRANSPORT THROUGH BIOMEMBRANES
    Robert Langdon; Fiscal Year: 1980
    ..2) Compare its site of reaction with that of affinity labels for the anion transporter, which is also present in Band 3...
  49. CARDIOPULMONARY ACTIONS--TRIMETOQUINOL ANALOGS
    Duane Miller; Fiscal Year: 1993
    ..The primary objective of this proposal is to synthesize and study highly selective photoaffinity and affinity labels of TMQ as probes of TXA2 and beta-adrenergic receptors, and to utilize mutant beta-adrenoceptor systems for ..
  50. SELECTIVE NONPEPTIDE OPIOID LIGANDS
    PHILIP PORTOGHESE; Fiscal Year: 1999
    ..kappa receptors without involvement of central kappa receptors; d) The design and synthesis of kappa receptor affinity labels that are structurally related to known kappa antagonists...
  51. SELECTIVE NONPEPTIDE OPIOID LIGANDS
    PHILIP PORTOGHESE; Fiscal Year: 2001
    ..kappa receptors without involvement of central kappa receptors; d) The design and synthesis of kappa receptor affinity labels that are structurally related to known kappa antagonists...
  52. SELECTIVE NONPEPTIDE OPIOID LIGANDS
    PHILIP PORTOGHESE; Fiscal Year: 2000
    ..kappa receptors without involvement of central kappa receptors; d) The design and synthesis of kappa receptor affinity labels that are structurally related to known kappa antagonists...
  53. ACTIVE SITE & MECHANISM OF RNA POLYMERASE
    ROBERT WOODY; Fiscal Year: 1990
    ..Nitration of tyrosine residues will be studied, especially those on the Sigma subunit. The site of affinity labels and photoaffinity labels will be determined in the sequence by chain separation cleavage and peptide mapping...
  54. A MECHANISTIC STUDY OF SOME O2 OXIDOREDUCTASES
    Gordon Hamilton; Fiscal Year: 1990
    ..To this end we plan to: further characterize and develop affinity labels for the enzyme's active site residues, obtain the complete amino acid sequence of the enzyme by cloning and ..
  55. AFFINITY LABELS FOR OPIOID RECEPTORS
    JANE ALDRICH; Fiscal Year: 1999
    ..A second objective is to identify peptide-based affinity labels for opioid receptors...
  56. PEPTIDE TRANSPORT IN MICROORGANISMS
    Fred Naider; Fiscal Year: 1980
    ..Such affinity labels will be used to covalently insert radioactive or chromogenic markers into components of the peptide transport ..