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Genomes and Genes | affinity labelsSummarySummary: Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids. Top Publications
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The Nano-tag, a streptavidin-binding peptide for the purification and detection of recombinant proteinsThorsten Lamla
, , Thielallee 63, D-14195 Berlin, Germany
Protein Expr Purif 33:39-47. 2004..Additionally, the Nano-tag allowed the detection of recombinant proteins on Western blots by a streptavidin-alkaline phosphatase conjugate...- An efficient tandem affinity purification procedure for interaction proteomics in mammalian cellsTilmann Burckstummer
Research Center for Molecular Medicine CeMM, Lazarettgasse 19 3, 1090 Vienna, Austria
Nat Methods 3:1013-9. 2006..Using the well-characterized Ku70-Ku80 protein complex as an example, we identified both core elements as well as new candidate effectors... - Use of fluorescently labeled caspase inhibitors as affinity labels to detect activated caspasesJerzy Grabarek
New York Medical College, Hawthorne, NY 10532, USA
Hum Cell 15:1-12. 2002..To detect their activation in situ, we applied fluorochrome labeled inhibitors of caspases (FLICA) as affinity labels of active centers of these enzymes... - A generic protein purification method for protein complex characterization and proteome explorationG Rigaut
European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Nat Biotechnol 17:1030-2. 1999..Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms... 
Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic stepS Chong
New England Biolabs Inc, 32 Tozer Road, Beverly, MA 01915, USA
Nucleic Acids Res 26:5109-15. 1998..We have constructed general cloning vectors and demonstrated single-column purification of several proteins. In addition, we discuss several factors that may affect the C-terminal peptide bond cleavage activity...- Sephadex-based cell-affinity adsorbents: preparation and performanceGeert Besselink
Department of Transfusion Technology, CLB, Sanquin Blood Supply Foundation, P.O. Box 9190, 1066 AC Amsterdam, The Netherlands
Biotechnol Appl Biochem 35:55-60. 2002..One obvious advantage of the approach described here is that relatively small amounts of SpA and antisera are needed for preparing cell-affinity media... - The First Nucleotide Binding Domain of Cystic Fibrosis Transmembrane Conductance Regulator Is a Site of Stable Nucleotide Interaction, whereas the Second Is a Site of Rapid TurnoverLuba Aleksandrov
Mayo Foundation and Mayo Clinic Scottsdale, S. C. Johnson Medical Research Center, Scottsdale, Arizona 85259, USA
J Biol Chem 277:15419-25. 2002..This demonstration of NBD2 as the rapid nucleotide turnover site is consistent with the strong effect on channel gating kinetics of inactivation of this domain by mutagenesis... - Efficient identification of photolabelled amino acid residues by combining immunoaffinity purification with MS: revealing the semotiadil-binding site and its relevance to binding sites for myristates in domain III of human serum albuminKohichi Kawahara
Department of Biofunctional Chemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, 5 1 Ohe Honmachi, Kumamoto 862 0973, Japan
Biochem J 363:223-32. 2002..These results indicate that FNAK labelling can also be used to monitor Myr binding in domain III. An interpretation for the concomitant local conformational change of HSA is provided... - New role for an old probe: affinity labeling of oxylipid protein conjugates by N'-aminooxymethylcarbonylhydrazino d-biotinJuan Chavez
Department of Chemistry, Oregon State University, Corvallis, Oregon 97331, USA
Anal Chem 78:6847-54. 2006..The reported approach is outlined for the detection, identification, and characterization of oxylipid peptide conjugates, but the labeling chemistry may also be applicable to other carbonyl-modified proteins... - Participation of yeast inosine 5'-monophosphate dehydrogenase in an in vitro complex with a fragment of the C-rich telomeric strandJean Francois Cornuel
Groupe de Biophysique de l UMR 7643 du CNRS, Ecole Polytechnique, 91128, Palaiseau, France
Biochimie 84:279-89. 2002..We note that the genes coding for Imd2p and Imd3p are located close to the telomere, and could therefore be subject to silencing by the telomere position effect... - Element-coded affinity tags for peptides and proteinsPaul A Whetstone
Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA
Bioconjug Chem 15:3-6. 2004..Further, the rare earths are heavy elements, whose mass defects give the masses of tagged peptides exact values not normally shared by molecules that contain only light elements... - Functional asymmetry of the two nucleotide binding domains in the ABC transporter Ste6C Proff
, , Germany
Mol Gen Genet 264:883-93. 2001..The experiments further suggest that the two NBDs of Ste6 are not equivalent and affect each other's ability to bind and hydrolyze ATP... - Identification of a contact domain between echistatin and the integrin alpha(v)beta(3) by photoaffinity cross-linkingL Scheibler
Division of Bone and Mineral Metabolism, Charles A. Dana and Thorndike Laboratories, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
Biochemistry 40:15117-26. 2001..These cross-linking data support the hypothesis that the ligand-bound conformation of the integrin beta(3) subunit differs from the known conformation of I domains... - DnaA protein Lys-415 is close to the ATP-binding site: ATP-pyridoxal affinity labelingT Kubota
Department of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
Biochem Biophys Res Commun 288:1141-8. 2001..Thus, this residue is likely close to the bound ATP. Since Lys-415 is located in the DNA-binding domain, these findings imply internal interaction between the domains for ATP binding and DNA binding... - Characterization of the nucleotide-binding capacity and the ATPase activity of the PIP3-binding protein JFC1S D Catz
Department of Molecular and Experimental Medicine, Division of Biochemistry, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 98:11230-5. 2001..Phosphatidylinositol 3,4,5-trisphosphate, a high-affinity ligand of JFC1 did not affect its ATPase kinetics parameters, suggesting that the phosphoinositide have a different role in JFC1 function... - Analysis of phosphoinositide-binding proteins using liposomes as an affinity matrixAndreas Knödler
Oregon Health and Science University, Portland, OR, USA
Biotechniques 38:858, 860, 862. 2005 - ATP analogue binding to the A subunit induces conformational changes in the E subunit that involves a disulfide bond formation in plant V-ATPaseY Kawamura
Cryobiosystem Research Center, Iwate University, Iwate, Japan
Eur J Biochem 268:2801-9. 2001..This is the first demonstration of interaction between the A and E subunits in the substrate-binding state of a plant V-ATPase... - Nucleotide-binding sites in the functional unit of sarcoplasmic reticulum Ca2+-ATPase as studied by photoaffinity spin-labeled 2-N3-SL-ATPT Palm
Fachbereich Chemie, , Germany
Biol Chem 382:417-23. 2001..This indicates that the minimal functional unit of the Ca2+-ATPase is a dimer with the nucleotide-binding sites in close proximity... - Functionally similar vanadate-induced 8-azidoadenosine 5'-[alpha-(32)P]Diphosphate-trapped transition state intermediates of human P-glycoprotin are generated in the absence and presence of ATP hydrolysisZ E Sauna
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-4255, USA
J Biol Chem 276:21199-208. 2001..alpha-(32)P]8-azido-ADP (or ADP).Vi transition state complexes generated either in the absence of or accompanying [alpha-(32)P]8-azido-ATP hydrolysis are functionally indistinguishable... - Variability in the immunodetection of His-tagged recombinant proteinsNatasa Debeljak
Laboratory for Cell and Molecular Biology, Division of Hematology and Oncology, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, 330 Brookline Ave, W/BL 548, Boston, MA 02215, USA
Anal Biochem 359:216-23. 2006..Such variability in His-tag immunorecognition can lead to critical adverse effects on several analytical methods... - Use of dual affinity tags for expression and purification of functional peripheral cannabinoid receptorAlexei Yeliseev
Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, 5625 Fishers Lane, Bethesda, MD 20892, USA
Protein Expr Purif 53:153-63. 2007..Preliminary results of reconstitution experiments indicate that the CB2 has retained its ligand-binding properties... - Identification of the bovine gamma-aminobutyric acid type A receptor alpha subunit residues photolabeled by the imidazobenzodiazepine [3H]Ro15-4513Gregory W Sawyer
Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, CA 90095, USA
J Biol Chem 277:50036-45. 2002..These results are discussed in terms of a homology model of the benzodiazepine-binding site based on the molluscan acetylcholine-binding protein structure... - The nucleotide-binding site of human sphingosine kinase 1Stuart M Pitson
Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Frome Road, Adelaide SA 5000, Australia
J Biol Chem 277:49545-53. 2002..It does, however, share some sequence and likely structural similarity with the highly conserved glycine-rich loop, which is known to be involved in anchoring and positioning the nucleotide in the catalytic site of many protein kinases... - Mapping sites of O-GlcNAc modification using affinity tags for serine and threonine post-translational modificationsLance Wells
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21218, USA
Mol Cell Proteomics 1:791-804. 2002..In addition, our studies emphasize the importance of distinguishing between O-phosphate versus O-GlcNAc when mapping sites of serine and threonine post-translational modification using beta-elimination/Michael addition methods... - Screening of peptide affinity tags using immobilised metal affinity chromatography in 96-well plate formatAmro Hanora
Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-22100 Lund, Sweden
J Chromatogr A 1087:38-44. 2005..From screening 25 different tags, three clones were able to bind to all metal ions studied (Ni2+, Zn2+, Co2+ and Cd2+). It was clearly demonstrated that the new construct could facilitate the screening of large peptide libraries... - Immobilisation of a repressor protein for binding of plasmid DNAAnja Hasche
Department of Fermentation Engineering, Faculty of Technology, Bielefeld University, Universitaetsstrasse 25, P.O. Box 100131, D-33501 Bielefeld, Germany
J Chromatogr A 1080:76-82. 2005.... - Triatoma infestans apyrases belong to the 5'-nucleotidase familyEric Faudry
Chagas' Disease Multidisciplinary Research Laboratory, Department of Pathology, Faculty of Medicine, , Brazil 70.910-900
J Biol Chem 279:19607-13. 2004.... - Application of protein-coupled liposomes to effective affinity screening from phage libraryYoichi Kumada
Graduate School of Science and Technology, Kobe University, Rokkodaicho 1-1, Nada, Kobe, Hyogo 657-8501, Japan
J Chromatogr A 1080:22-8. 2005..Thus, protein-coupled liposomes are useful as adsorbents for screening from combinatorial phage libraries... - Application of isotope coded affinity tag (ICAT) analysis for the identification of differentially expressed proteins following infection of atlantic salmon (Salmo salar) with infectious hematopoietic necrosis virus (IHNV) or Renibacterium salmoninarum (BA T Booy
University of Victoria, Genome British Columbia Proteomics Centre, 3101-4464 Markham Street, Victoria, British Columbia, Canada V8Z 7X8
J Proteome Res 4:325-34. 2005..Examples of the latter are discussed here and include, a natural killer cell enhancement factor (NKEF), procathepsin L, superoxide-producing NADPH oxidase and interferon-induced viral resistance protein Mx (IFI-Mx)... - Rapid evaluation of nickel binding properties of His-tagged lactate dehydrogenases using surface plasmon resonanceFlorent Bernaudat
Department of Pure and Applied Biochemistry, Centre for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, Getingevagen 60, 221 00 Lund, Sweden
J Chromatogr A 1066:219-24. 2005..Protein modelling has also proved to be useful in supporting the experimental results... - Affinity labeling the dopamine transporter ligand binding siteRoxanne A Vaughan
Department of Biochemistry and Molecular Biology, University of North Dakota, School of Medicine and Health Sciences, 501 N Columbia Road, Grand Forks, ND 58203, USA
J Neurosci Methods 143:33-40. 2005..Using these methods we have identified two distinct regions of DAT that interact with multiple structurally related and diverse irreversible ligands, suggesting that these regions may be involved in the formation of ligand binding sites... - [Tagged-MS method]Ryoichi Arai
Tanpakushitsu Kakusan Koso 49:2763-7. 2004 - Investigation of the abundance and subunit composition of GABAA receptor subtypes in the cerebellum of alpha1-subunit-deficient miceWaltraud Ogris
Division of Biochemistry and Molecular Biology, Center for Brain Research, and Section of Biochemical Psychiatry, University Clinic for Psychiatry, Medical University Vienna, Vienna, Austria
J Neurochem 96:136-47. 2006..Our results do not support a significant compensatory synthesis of other GABAA receptor subunits in the cerebellum of alpha1 -/- mice... - Proteomic analysis of the binding partners to enteropathogenic Escherichia coli virulence proteins expressed in Saccharomyces cerevisiaePhilip R Hardwidge
Veterinary Science Department, South Dakota State University, Brookings, SD, USA
Proteomics 6:2174-9. 2006..The dataset suggests several potential mammalian targets of these proteins that may guide future experimentation... - Affinity labeling of the rabbit 12/15-lipoxygenase using azido derivatives of arachidonic acidStepan Romanov
Institute of Biochemistry, University Medicine Berlin-Charit, Monbijoustrasse 2, 10117 Berlin, Germany
Biochemistry 45:3554-62. 2006.... - The affinity concept in bioseparation: evolving paradigms and expanding range of applicationsKalyani Mondal
Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India
Biomol Eng 23:59-76. 2006..At industrial level, validation, biosafety and process hygiene are also important aspects. This overview looks at these evolving paradigms and various strategies which utilize affinity phenomenon for protein separations... - A cleavable affinity biotinylating agent reveals a retinoid binding role for RPE65Wan Jin Jahng
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 45 Shattuck Street, Boston, Massachusetts 02115, USA
Biochemistry 42:6159-68. 2003..These studies validate the approach that was used, and furthermore demonstrate that RPE65, a major membrane-associated protein of the RPE, is a RBP... - Analysis of transglutaminase protein substrates by functional proteomicsMargherita Ruoppolo
Dipartimento di Biochimica e Biotecnologie Mediche, Universita' di Napoli Federico II, Napoli, Italy
Protein Sci 12:1290-7. 2003..Results obtained in this paper indicate that the whole strategy can be successfully applied in order to identify transglutaminases protein substrates as well as the amino acid site sensitive toward enzyme activity... - Differential effects of supplementary affinity tags on the solubility of MBP fusion proteinsKaren M Routzahn
Protein Engineering Section, Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute at Frederick, P.O. Box B, Frederick, Maryland 21702, USA
J Struct Funct Genomics 2:83-92. 2002.... - Functional interaction between the two halves of the photoreceptor-specific ATP binding cassette protein ABCR (ABCA4). Evidence for a non-exchangeable ADP in the first nucleotide binding domainJinhi Ahn
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
J Biol Chem 278:39600-8. 2003..Our results indicate that only NBD2 of ABCR binds and hydrolyzes ATP in the presence or absence of retinal. NBD1, containing a bound ADP, associates with NBD2 to play a crucial, non-catalytic role in ABCR function... - The structure of the mammalian signal recognition particle (SRP) receptor as prototype for the interaction of small GTPases with Longin domainsOliver Schlenker
, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany
J Biol Chem 281:8898-906. 2006..Based on structural conservation we present the SRbeta-GTP:SRX structure as a prototype for conserved interactions in a variety of GTPase regulated targeting events occurring at endomembranes... - Synthesis and screening of a rationally designed combinatorial library of affinity ligands mimicking protein L from Peptostreptococcus magnusA Cecília A Roque
Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisboa, Portugal
J Mol Recognit 18:213-24. 2005..The most promising lead, ligand 8/7, when immobilized on an agarose support, behaved in a similar fashion to PpL in isolating Fab fragments from papain digests of human IgG to a final purity of 97%... - Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid taggingDaniela C Dieterich
Division of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
Nat Protoc 2:532-40. 2007..This protocol can be completed in 5 days... - A single histidine in GABAA receptors is essential for benzodiazepine agonist bindingH A Wieland
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, Germany
J Biol Chem 267:1426-9. 1992..Hence, this histidine present in the alpha 1, alpha 2, alpha 3, and alpha 5 subunits appears to be a key residue for the action of clinically used BZ ligands... - Selection of optimum affinity tags from a phage-displayed peptide library. Application to immobilized copper(II) affinity chromatographyA V Patwardhan
Department of Chemical Engineering, University of Pittsburgh, PA 15219, USA
J Chromatogr A 787:91-100. 1997.... - The first phospholipase inhibitor from the serum of Vipera ammodytes ammodytesJernej Sribar
Department of Molecular and Biomedical Sciences, Jozef Stefan Institute, Ljubljana, Slovenia
FEBS J 274:6055-64. 2007.... - Photoaffinity labeling of terminal deoxynucleotidyl transferase. 2. Identification of peptides in the nucleotide binding domainR K Evans
Department of Biochemistry, University of Kentucky, Lexington 40536 0084
Biochemistry 28:713-20. 1989..Structure predictions, based on sequence data, place the two peptides identified by photolabeling in spatial proximity consistent with the participation of both in the nucleotide binding domain... - Catalytic and noncatalytic nucleotide binding sites of the Escherichia coli F1 ATPase. Amino acid sequences of beta-subunit tryptic peptides labeled with 2-azido-ATPJ G Wise
Department of Chemistry and Biochemistry, University of California, Los Angeles 90024 1570
FEBS Lett 223:395-401. 1987..These beta-subunit peptides were labeled on tyrosine-331 (catalytic sites) and tyrosine-354 (noncatalytic sites) in homology with the labeling patterns of the mitochondrial and chloroplast enzymes... - Photoaffinity labeling of human placental S-adenosylhomocysteine hydrolase with [2-3H]8-azido-adenosineC S Yuan
Department of Biochemistry, University of Kansas, Lawrence 66045, USA
J Biol Chem 270:16140-6. 1995..The peptide Val319-Arg327 is adjacent to Leu330, which is proposed by a computer graphics model to interact with the C-6-NH2 group of Ado... - Identification of the ubiquinone-binding domain in QPs1 of succinate-ubiquinone reductaseG Y Lee
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078
J Biol Chem 270:6193-8. 1995..The Q-binding domain, using the proposed structure of QPs1, is probably located in the stretch connecting transmembrane helices 2 and 3 that extrude from the surface of the M side of the inner membrane... - Spermidine-preferential uptake system in Escherichia coli. ATP hydrolysis by PotA protein and its association with membraneK Kashiwagi
Faculty of Pharmaceutical Sciences, Chiba University, Japan
J Biol Chem 270:25377-82. 1995..ATPase of PotA protein was inhibited by spermidine, suggesting that uptake inhibition by spermidine may function during this process... - The proteins encoded by the rbs operon of Escherichia coli: II. Use of chimeric protein constructs to isolate and characterize RbsCJ Zaitseva
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907 1153, USA
Protein Sci 5:1100-7. 1996..This is a substantial increase in amounts from any previous RbsC production vectors. All proteins from the rbs operon have now been overproduced and substantially purified... - The nucleotide-binding site of HisP, a membrane protein of the histidine permease. Identification of amino acid residues photoaffinity labeled by 8-azido-ATPC S Mimura
Department of Molecular and Cell Biology, University of California, Berkeley 94720
J Biol Chem 265:19535-42. 1990..These consensus motifs are found in many purine nucleotide-binding proteins. The relationship between the location of these residues and the overall structure of the ATP-binding site is discussed... - Characterization of KpsT, the ATP-binding component of the ABC-transporter involved with the export of capsular polysialic acid in Escherichia coli K1M S Pavelka
Department of Microbiology and Immunology, University of Rochester Medical Center, New York 14642
J Biol Chem 269:20149-58. 1994..The results obtained from chemical mutagenesis of kpsT are consistent with the model and revealed characteristics particular to capsule transporters... - Analysis of a nucleotide-binding site of 5-lipoxygenase by affinity labelling: binding characteristics and amino acid sequencesY Y Zhang
Whitaker Cardiovascular Institute, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
Biochem J 351:697-707. 2000..Given the stoichiometry of the labelling, the two peptide sequences of 5LO were probably near each other in the enzyme's tertiary structure, composing or surrounding the ATP-binding site of 5LO... - Natural poly-histidine affinity tag for purification of recombinant proteins on cobalt(II)-carboxymethylaspartate crosslinked agaroseG Chaga
CLONTECH Laboratories, Inc, Palo Alto, CA 94303, USA
J Chromatogr A 864:247-56. 1999..Recovery for each purified protein was higher than 77% of the initial loaded amount as judged by biological activity. The operational capacity of Co2+-carboxymethylaspartate agarose for each protein was determined... - Novel mode of ligand binding by the SH2 domain of the human XLP disease gene product SAP/SH2D1AS C Li
Program in Molecular Biology and Cancer, Department of Molecular and Medical Genetics, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, Canada
Curr Biol 9:1355-62. 1999..The SAP-SLAM interaction can occur in a phosphorylation-independent manner... - Histidines in affinity tags and surface clusters for immobilized metal-ion affinity chromatography of trimeric tumor necrosis factor alphaV Gaberc-Porekar
National Institute of Chemistry, Hajdrihova, Ljubljana, Slovenia
J Chromatogr A 852:117-28. 1999..Histidines, relatively rigidly inserted in the structure, as in our model proteins, display superior chromatographic characteristics vis a vis flexible tags with the same total number of histidines... - The quinone-binding site in succinate-ubiquinone reductase from Escherichia coli. Quinone-binding domain and amino acid residues involved in quinone bindingX Yang
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
J Biol Chem 273:31916-23. 1998..The hydroxyl group, but not the size of the amino acid side chain, at position 33 of SdhC is also important, because Ser-33 can be substituted with threonine but not with alanine... - Identification of proteins binding to interferon-inducible transcriptional enhancers in hematopoietic cellsA Wedrychowski
Department of Hematology, University of Texas M D Anderson Cancer Center, Houston 77030
J Biol Chem 267:4533-40. 1992..Following exposure to alpha-interferon, more slowly migrating complexes appeared which contained a 48-kDa protein, a 95-kDa protein, and a 105-kDa protein which bound to the 9-27 transcriptional enhancer in a sequence-specific manner... - Distribution of GLUT3 glucose transporter protein in human tissuesP R Shepherd
Charles A Dana Research Institute, Beth Israel Hospital, Boston, MA 02215
Biochem Biophys Res Commun 188:149-54. 1992..No GLUT3 was detected in membranes from any of 3 skeletal muscle groups investigated. We conclude that a major role of GLUT3 in humans is as the brain neuronal glucose transporter... - Photolabeling of the phosphate binding site of chloroplast coupling factor 1 with [32P]azidonitrophenyl phosphateL Michel
Laboratoire de Biochimie URA 1130 CNRS, Departement de Biologie Moleculaire et Structurale, Centre d Etudes Nucléaires, Grenoble, France
FEBS Lett 313:90-3. 1992..The labeled beta-Tyr328 of CF1 is the equivalent of beta-Tyr311 of F1 from beef heart mitochondria, which was previously found to be photolabeled by ANPP [J. Garin et al. (1989) Biochemistry 28, 1442-1448]... - Improved anti-IgG and HSA affinity ligands: clinical application of VHH antibody technologyRinse Klooster
Department of Cellular Architecture and Dynamics, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
J Immunol Methods 324:1-12. 2007..Moreover, as VHHs are single protein chains, they can be coupled relatively easily to solid matrices. These three factors are important for developing affinity purification medication... - Neuropharmacology of the naturally occurring kappa-opioid hallucinogen salvinorin AChristopher W Cunningham
Department of Medicinal Chemistry, University of Kansas, Lawrence, KS 66045 7582, USA
Pharmacol Rev 63:316-47. 2011..salvinorin A responsible for opioid receptor affinity and selectivity has produced numerous receptor probes, affinity labels, and tools for evaluating the biological processes responsible for its observed psychological effects... - The molecular basis for the mode of action of bicyclomycinHarold Kohn
Division of Medicinal Chemistry and Natural Products, School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7360, USA
Curr Drug Targets Infect Disord 5:273-95. 2005..obtained from bicyclomycin structure-activity studies, site-directed mutagenesis investigations, bicyclomycin affinity labels, and biochemical and biophysical measurements with recent X-ray crystallographic images of the bicyclomycin-.. - Affinity labeling of oxaloacetate decarboxylase by novel dichlorotriazine linked alpha-ketoacidsN E Labrou
Department of Agricultural Biotechnology, Agricultural University of Athens, Greece
J Protein Chem 18:729-33. 1999..and beta-mercaptopyruvic acid linked to the reactive diclorotriazine ring, were studied as active site-direct affinity labels towards oxaloacetate decarboxylase (EC 4.1.1.3, OXAD)... - 2-Dialkynyl derivatives of (N)-methanocarba nucleosides: 'Clickable' A(3) adenosine receptor-selective agonistsDilip K Tosh
Molecular Recognition Section, Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bldg 8A, Rm B1A 19, NIH, NIDDK, LBC, Bethesda, MD 20892, United States
Bioorg Med Chem 18:508-17. 2010..Other potent, selective derivatives (K(i) at A(3)AR innM) were intended as possible receptor affinity labels: 3-nitro-4-fluorophenyl (10.6), alpha-bromophenacyl (9... - Cutting edge: FcR-like 5 on innate B cells is targeted by a poxvirus MHC class I-like immunoevasinJessica A Campbell
Division of Pediatric Rheumatology, Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
J Immunol 185:28-32. 2010..Due to their binding specificities, immunoevasins can be exploited as affinity labels to identify host-encoded molecules of previously unsuspected importance in defense against the relevant class .. - Cysteine proteinase inhibitors as therapy for parasitic diseases: advances in inhibitor designDietmar Steverding
Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich NR4 7TJ, UK
Mini Rev Med Chem 6:1025-32. 2006..Also, the development of activity-based affinity labels has enabled the identification and characterization of potential cysteine proteinase targets in situ... - Affinity labels as tools for the identification of opioid receptor recognition sitesP S Portoghese
Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA
Farmaco 56:191-6. 2001b>Affinity labels have proven to be useful tools in opioid research... - Discovery of halopyridines as quiescent affinity labels: inactivation of dimethylarginine dimethylaminohydrolaseCorey M Johnson
Division of Medicinal Chemistry, College of Pharmacy, University of Texas, Austin, Texas 78712, United States
J Am Chem Soc 133:1553-62. 2011..To our knowledge, halopyridines have not previously been reported as protein modifiers, and therefore they represent a first-in-class example of a novel type of quiescent affinity label... - Analogs of Manduca adipokinetic hormone tested in a bioassay and in a receptor-binding assayR Ziegler
Department of Biochemistry and Center for Insect Science, University of Arizona, Tucson 85721, USA
Peptides 19:481-6. 1998..In addition an extended M-AKH and analogs containing photo affinity labels were tested. All analogs had reduced activity... 
The thyroid hormone transporters MCT8 and MCT10 transport the affinity-label N-bromoacetyl-[(125)I]T3 but are not modified by itW Edward Visser
Department of Internal Medicine, Erasmus University Medical Center, Dr Molewaterplein, Rotterdam, The Netherlands
Mol Cell Endocrinol 337:96-100. 2011..The usefulness of BrAc[(125)I]T3 as a tool for the identification of novel TH transporters remains to be explored...- Immunoassays based on electrochemical detection using microelectrode arraysK Dill
CombiMatrix Corporation, Harbour Pointe Tech Center, 6500 Harbour Heights Parkway, Suite 301, Mukilteo, WA 98275, USA
Biosens Bioelectron 20:736-42. 2004..Antibodies are tagged with coded affinity labels and then allowed to self-assemble on the appropriate electrode assay sites... - Selective chemical labeling of proteins in living cellsLawrence W Miller
Department of Chemistry, Columbia University, Havemeyer Hall, MC 3153, 3000 Broadway, New York, New York 10027, USA
Curr Opin Chem Biol 9:56-61. 2005Labeling proteins with fluorophores, affinity labels or other chemically or optically active species is immensely useful for studying protein function in living cells or tissue... - Disparate proteome reactivity profiles of carbon electrophilesEranthie Weerapana
The Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Nat Chem Biol 4:405-7. 2008..reactivity of electrophiles are crucial for designing activity-based probes for enzymes lacking cognate affinity labels. Here, we show that different classes of carbon electrophiles exhibit markedly distinct amino acid labeling .. - Protein-inorganic array construction: design and synthesis of the building blocksNiculina D Bogdan
Department of Chemistry, Marie Curie Excellence Team, Technical University Munchen, 4 Lichtenberg Str 85748 Garching, Germany
Chemistry 16:2170-80. 2010..the way to the synthesis of di-functional ligands containing more elaborated polypyridine ligands as well as affinity labels for different enzyme families... - Synthesis and spectroscopic characterization of photo-affinity peptide ligands to study rhodopsin-G protein interactionYihui Chen
Department of Chemistry, Columbia University, New York, NY, USA
Photochem Photobiol 84:831-8. 2008..Here, we describe novel peptides with photo-affinity labels that bind R* with affinities similar to that of the native G(t)gamma(60-71)far peptide... - An efficient, one-pot synthesis of various ceramide 1-phosphates from sphingosine 1-phosphatePeter Nussbaumer
Novartis Institutes for BioMedical Research, Brunner Strasse 59, A 1235 Vienna, Austria
Chem Phys Lipids 151:125-8. 2008..Thus, ceramide 1-phosphates with various fatty acid chains and with fluorescent and affinity labels attached to the sphingoid backbone were prepared in good yields. - The mitochondrial permeability transition porePaolo Bernardi
Department of Biomedical Sciences and CNR Institute of Neurosciences, University of Padova, Viale Giuseppe Colombo 3, I 35121 Padova, Italy
Novartis Found Symp 287:157-64; discussion 164-9. 2007..While the nature of the components forming the PTP remains controversial, the identification of novel inhibitors that can be used as affinity labels is offering new perspectives towards the molecular definition of the PTP. - Short oligonucleotide probes containing G-stacks display abnormal binding affinity on Affymetrix microarraysChunlei Wu
Genomic Institute of Novartis Research Foundation, 10675 John Jay Hopkins Dr, San Diego, CA 92121, USA
Bioinformatics 23:2566-72. 2007..Our findings are expected to be useful in microarray design and data analysis... - Peptide tags for enhanced cellular and protein adhesion to single-crystalline sapphireEric M Krauland
Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Biotechnol Bioeng 97:1009-20. 2007..Targeting proteins to metal oxide surfaces with peptide tags may provide a facile one-step alternative coupling chemistry for the formation of protein bioassays and biosensors... - Nucleoside transporter expression and activity is regulated during granulocytic differentiation of NB4 cells in response to all-trans-retinoic acidSheryl A Flanagan
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Leuk Res 31:955-68. 2007.... - The pST44 polycistronic expression system for producing protein complexes in Escherichia coliSong Tan
Center for Gene Regulation, Department of Biochemistry and Molecular Biology, 108 Althouse Laboratory, The Pennsylvania State University, University Park, PA 16802 1014, USA
Protein Expr Purif 40:385-95. 2005..We also utilize the modular design to show that the order of expression of the three subunits along the polycistronic plasmid does not affect the reconstitution of the yeast Piccolo complex in E. coli... - Inhibition of multidrug resistance-linked P-glycoprotein (ABCB1) function by 5'-fluorosulfonylbenzoyl 5'-adenosine: evidence for an ATP analogue that interacts with both drug-substrate-and nucleotide-binding sitesShinobu Ohnuma
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4256, USA
Biochemistry 50:3724-35. 2011..Thus, FSBA is an ATP analogue that interacts with both the drug-binding and ATP-binding sites of P-gp, but fluorosulfonyl-mediated cross-linking is observed only at the NBDs... - The chemistry of irreversible captureClaude F Meares
Department of Chemistry, University of California, Davis, California 95616, USA
Adv Drug Deliv Rev 60:1383-8. 2008..These are based on a combination of genetic manipulation of the protein and chemical synthesis of appropriate ligands, examples of which are reviewed here... - Capillary electrophoresis-based noncompetitive immunoassay for the prion protein using fluorescein-labeled protein A as a fluorescent probeWen-Chu Yang
Department of Chemistry, Iowa State University, Ames, Iowa 50011, Ames Laboratory USDOE, Iowa State University, Ames, Iowa 50011, USA
Anal Chem 77:4489-94. 2005..9969. The estimated detection limit for rPrP was approximately 6 ng/mL, which is 3 times the signal-to-noise ratio. The method was successfully applied for testing blood samples from scrapie-infected sheep... - Low concentrations of ethanol do not affect radioligand binding to the delta-subunit-containing GABAA receptors in the rat brainAshok K Mehta
Department of Pharmacology, MC 7764, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229 3900, USA
Brain Res 1165:15-20. 2007..These results suggest that the native delta-subunit-containing GABA(A) receptors do not play a major role in the pharmacology of clinically relevant low concentrations of ethanol... - Covalent labeling of nuclear vitamin D receptor with affinity labeling reagents containing a cross-linking probe at three different positions of the parent ligand: structural and biochemical implicationsTaner Kaya
Boston University, Boston, MA 02215, USA
Bioorg Chem 37:57-63. 2009..Therefore, we conclude that the beta-hairpin region (modified by 1,25(OH)(2)D(3)-3-BE) is most important for growth inhibition by 1,25(OH)(2)D(3), while helices 3 and 6 are less important for such activity... - Proteomics in medicinal chemistrySatomi Niwayama
Department of Chemistry and Biochemistry, Texas Tech University, Box 41061, Lubbock, 79409 1061, USA
Mini Rev Med Chem 6:241-6. 2006..An important aspect of proteomics is to identify and quantify proteins. Many new technologies and techniques have been developed in this field and have been applied to various aspects of drug discovery... - Affinity-tagged green fluorescent protein (GFP) extraction from a clarified E. coli cell lysate using a two-phase aqueous micellar systemPriscila G Mazzola
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Biotechnol Bioeng 93:998-1004. 2006.... - Utility of cleavable isotope-coded affinity-tagged reagents for quantification of low-copy proteins induced by methylprednisolone using liquid chromatography/tandem mass spectrometryJun Qu
The Department of Pharmaceutical Sciences, University at Buffalo, State University of New York, Amherst, New York 14260-1200, USA
Anal Chem 78:4543-52. 2006..The approach enables simultaneous quantification of multiple effector proteins induced by biological or pharmacological stimuli, and the processed samples can be interrogated repeatedly as additional targets of interest arise... - Isolation of cell specific peptide ligands using fluorescent bacterial display librariesKaren Y Dane
Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA
J Immunol Methods 309:120-9. 2006..Fluorescent display libraries thus provide a powerful new methodology for parallel identification of cell specific affinity ligands... - Identification of intracellular proteins associated with the EBV-encoded nuclear antigen 5 using an efficient TAP procedure and FT-ICR mass spectrometryAlma Forsman
Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska Academy, Sahlgrenska University Hospital, University of Gothenburg, Gothenburg, Sweden
J Proteome Res 7:2309-19. 2008..Our study also confirms previously identified interactors including HA95, Hsp70, Hsc70, Hsp27, HAX-1, Prolyl 4-hydroxylase, S3a, and alpha- and beta-tubulin... - Development of rationally designed affinity-based drug delivery systemsDustin J Maxwell
Department of Biomedical Engineering, Washington University, Campus Box 1097, 1 Brookings Drive, St. Louis, MO 63130-4899, USA
Acta Biomater 1:101-13. 2005..These studies demonstrate that our rational approach to drug delivery system design can be used to control drug release from tissue-engineered scaffolds and may be useful for promoting tissue regeneration in vivo... - Evaluation of homologous, heterologous, and affinity conjugates for the serodiagnosis of Toxoplasma gondii and Neospora caninum in maned wolves (Chrysocyon brachyurus)D A O Silva
Laboratory of Immunoparasitology, , MG, Brasil
J Parasitol 91:1212-6. 2005..gondii in wild canids, whereas for N. caninum infection, only the homologous or heterologous fluorescent conjugates have been shown to be useful... - Sequential ATP hydrolysis by Cdc6 and ORC directs loading of the Mcm2-7 helicaseJohn C W Randell
Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Mol Cell 21:29-39. 2006..Importantly, these coordinated yet distinct functions of ORC and Cdc6 ensure the correct temporal and spatial regulation of pre-RC formation... - Affinity peptide for targeted detection of dysplasia in Barrett's esophagusMeng Li
Division of Gastroenterology, Department of Medicine, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi an, Shaanxi, People s Republic of China
Gastroenterology 139:1472-80. 2010..We aim to select and validate an affinity peptide that binds to esophageal dysplasia for future clinical studies... - Identification of Tyr115 labeled by S-(4-bromo-2,3-dioxobutyl)glutathione in the hydrophobic substrate binding site of glutathione S-transferase, isoenzyme 3-3R M Katusz
Department of Chemistry and Biochemistry, University of Delaware, Newark 19716
Arch Biochem Biophys 298:667-77. 1992.... - Site-specific crosslinks of yeast U6 snRNA to the pre-mRNA near the 5' splice siteC H Kim
Division of Biology, California Institute of Technology, Pasadena 91125, USA
RNA 2:995-1010. 1996..This experiment is a paradigm for future studies in which different mutant extracts are used to establish the stage in assembly at which particular RNA-RNA interactions defined by unique crosslinks occur... - Generation of bioreagents for protein chipsMichael L Phelan
BD Biosciences Clontech, Palo Alto, CA, USA
Proteomics 3:2123-34. 2003..New technologies for the production of protein capture agents are more amenable to automation than traditional monoclonal antibody production and therefore carry the promise for industrialization...
Research Grants
- PORPHOBILINOGEN SYNTHASE, PROBES OF THE ACTIVE SITEEileen Jaffe; Fiscal Year: 1993..addition products and characterize their behavior as alternative substrates, reversible inhibitors, or affinity labels of PBGS...
- AFFINITY LABELS FOR INOSITOL POLYPHOSPHATE RECEPTORSGlenn Prestwich; Fiscal Year: 1999..The affinity reagents and the binding domain data obtained from these reagents will be important in defining the mechanisms of these ubiquitous and varied molecular switches. ..
- Structural basis of specificity in affinity-labeled polyketide synthase didomainsJanet L Smith; Fiscal Year: 2011..b>Affinity labels have been demonstrated to be an effective means to establish the mechanism of pikromycin thioesterase (Pik TE)..
- Structural basis of specificity in affinity-labeled polyketide synthase didomainsJanet L Smith; Fiscal Year: 2010..b>Affinity labels have been demonstrated to be an effective means to establish the mechanism of pikromycin thioesterase (Pik TE)..
- BIOCHEMICAL CHARACTERIZATION OF OPIOID BINDING SITESGavril Pasternak; Fiscal Year: 1993..Finally, we will extend our studies utilizing affinity labels synthesized in our laboratory to investigate binding heterogeneity at the molecular level...
- STRUCTURAL STUDIES ON THE ENERGY TRANSDUCING ATPASEWilliam Allison; Fiscal Year: 1980..Analogues of ATP that are potential affinity labels for the enzyme will be synthesized for these studies...
- Sigma Receptor Structure /Function /K+ Channel ModulationARNOLD RUOHO; Fiscal Year: 2007..1) synthesis and characterization of novel high affinity sigma receptor agonist and antagonist photo affinity labels; (2) mapping the ligand binding site(s) of the sigma1 receptor; and (3) determination of the properties of ..
- EPOTHILONE AFFINITY LABELSGunda Georg; Fiscal Year: 2003..It is the plan to prepare epothilone affinity labels through emisynthesis and total synthesis and evaluate them for tubulin assembly activity and cytotoxicity...
- Liquid Chormatograph Mass Spectrometer (LC-MS)MURRAY JOHNSTON; Fiscal Year: 2002..These projects include: (Colman) characterization of new affinity labels, chemically modified enzymes and purified modified peptides and mutant enzymes; (Koh) selective regulation of ..
- SUSTRATE SPECIFICITY OF NONRECEPTOR TYROSINE KINASEW Miller; Fiscal Year: 2003..substrate analogs for these enzymes containing the photoactive amino acid p-benzoyl-Phe will be used as the affinity labels. Modified regions inside and outside the SH2 and SH3 domains will be studied further by site-directed ..