Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugsH Jomaa
Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, Friedrichstrasse 24, D 35392 Giessen, Germany
Science 285:1573-6. 1999
..Both drugs suppressed the in vitro growth of multidrug-resistant P. falciparum strains. After therapy with these drugs, mice infected with the rodent malaria parasite P. vinckei were cured...
Isoprenoid biosynthesis in Synechocystis sp. strain PCC6803 is stimulated by compounds of the pentose phosphate cycle but not by pyruvate or deoxyxylulose-5-phosphateYuri V Ershov
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA
J Bacteriol 184:5045-51. 2002
..strain PCC6803. Our data support alternative routes of entry of pentose phosphate cycle substrates derived from photosynthesis...
- 1-Deoxy-D-xylulose 5-phosphate reductoisomerase (IspC) from Mycobacterium tuberculosis: towards understanding mycobacterial resistance to fosmidomycin
Rakesh K Dhiman
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
J Bacteriol 187:8395-402. 2005
..Thus, M. tuberculosis resistance to fosmidomycin is not due to intrinsic properties of Rv2870c, and the enzyme appears to be a valid drug target in this pathogen...
- Structural basis of fosmidomycin action revealed by the complex with 2-C-methyl-D-erythritol 4-phosphate synthase (IspC). Implications for the catalytic mechanism and anti-malaria drug development
Stefan Steinbacher
Max Planck Institut fur Biochemie, Abteilung für Strukturforschung, Am Klopferspitz 18a, D 82152 Martinsried, Germany
J Biol Chem 278:18401-7. 2003
..Both sites are connected by a spacer of three methylene groups. The substrate molecule, 1-d-deoxyxylulose 5-phosphate, can be superimposed onto fosmidomycin, explaining the stereochemical course of the reaction...
- Chlorophyta exclusively use the 1-deoxyxylulose 5-phosphate/2-C-methylerythritol 4-phosphate pathway for the biosynthesis of isoprenoids
J Schwender
Botanisches Institut, Lehrstuhl II, , Germany
Planta 212:416-23. 2001
....
- E. coli MEP synthase: steady-state kinetic analysis and substrate binding
Andrew T Koppisch
Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
Biochemistry 41:236-43. 2002
..Fosmidomycin was an uncompetitive inhibitor against NADPH and gave a pattern representative of slow, tight-binding competitive inhibition against DXP. These results are consistent with an ordered mechanism where NADPH binds before DXP...
- Multistate binding in pyridoxine 5'-phosphate synthase: 1.96 A crystal structure in complex with 1-deoxy-D-xylulose phosphate
Joanne I Yeh
Molecular Biology, Cell Biology, and Biochemistry Department, Brown University, Box G J2, Providence, RI 02912 9108, USA
Biochemistry 41:11649-57. 2002
....
- The metabolic significance of octulose phosphates in the photosynthetic carbon reduction cycle in spinach
John F Williams
Research School of Chemistry, Australian National University, Canberra, A C T, 0200, Australia
Photosynth Res 90:125-48. 2006
....
- Isoprenoid biosynthesis via the methylerythritol phosphate pathway. Mechanistic investigations of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase
Jean François Hoeffler
Université Louis Pasteur CNRS, Institut Le Bel, Strasbourg, France
Eur J Biochem 269:4446-57. 2002
..The equilibrium was, however, largely displaced in favour of the formation of MEP. The reduction step required the presence of a divalent cation such as Mg(2+) or Mn(2+)...
- Catalytic by-product formation and ligand binding by ribulose bisphosphate carboxylases from different phylogenies
F Grant Pearce
School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8020, New Zealand
Biochem J 399:525-34. 2006
..Our observations also illustrate the phylogenetic diversity of mechanisms for regulating Rubisco and raise questions about whether an activase-like mechanism should be expected outside the green-algal/higher-plant lineage...
- Two methods for determination of transketolase activity
I A Sevostyanova
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia
Biochemistry (Mosc) 71:560-2. 2006
..It is not needed in the second method. The range of transketolase concentration in the activity assay is 0.036-0.144 U/ml for the first method and 1.8-6.8 U/ml for the second one...
- Probing catalysis by Escherichia coli dTDP-glucose-4,6-dehydratase: identification and preliminary characterization of functional amino acid residues at the active site
A D Hegeman
Department of Biochemistry, University of Wisconsin Madison, 53705, USA
Biochemistry 40:6598-610. 2001
..Additional steady-state techniques were employed in an attempt to further limit the assignment of rate limitation. The results are discussed within the context of the 4,6-dehydratase active site model and chemical mechanism...
- Structures of Aquifex aeolicus KDO8P synthase in complex with R5P and PEP, and with a bisubstrate inhibitor: role of active site water in catalysis
J Wang
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
Biochemistry 40:15676-83. 2001
..Overall, the process can be described as a syn addition of water and A5P to the si side of PEP...
- Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase complexed with cofactors: implications of a flexible loop movement upon substrate binding
Shunsuke Yajima
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
J Biochem 131:313-7. 2002
..A flexible loop covering the substrate binding site plays an important role in the enzymatic reaction and the determination of substrate specificity...
- Nudix hydrolases that degrade dinucleoside and diphosphoinositol polyphosphates also have 5-phosphoribosyl 1-pyrophosphate (PRPP) pyrophosphatase activity that generates the glycolytic activator ribose 1,5-bisphosphate
David I Fisher
Cell Regulation and Signalling Group, School of Biological Sciences, University of Liverpool, Liverpool L69 7ZB, United Kingdom
J Biol Chem 277:47313-7. 2002
..radiodurans DR2356 enzyme and for the DIPP subfamily. In the latter case, generation of the glycolytic activator ribose 1,5-bisphosphate may be a new function for these enzymes...
- Function of His185 in Aquifex aeolicus 3-deoxy-D-manno-octulosonate 8-phosphate synthase
Jian Wang
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
J Mol Biol 324:205-14. 2002
..aeolicus KDO8PS is to orient PEP in the active site of the enzyme in such a way that a water molecule on the sinister (si) side of PEP can be activated by direct coordination to the metal ion...
- Detection of novel enzyme intermediates in PEP-utilizing enzymes
Karen S Anderson
Department of Pharmacology, SHM B350B, Yale University School of Medicine, 333 Cedar Street New Haven, CT 06520, USA
Arch Biochem Biophys 433:47-58. 2005
..A novel application of this approach using time-resolved electrospray mass spectrometry to detect chemically labile enzyme intermediates is also discussed...
- The methylerythritol phosphate pathway is functionally active in all intraerythrocytic stages of Plasmodium falciparum
Maria B Cassera
Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo, 05508 900 Sao Paulo, Brazil
J Biol Chem 279:51749-59. 2004
..falciparum, and de novo biosynthesis of pyridoxal in a protozoan is reported. Its absence in the human host makes both pathways very attractive as potential new targets for antimalarial drug development...
- A novel NADH-linked l-xylulose reductase in the l-arabinose catabolic pathway of yeast
Ritva Verho
VTT Biotechnology, P. O. Box 1500, FIN-02044 VTT, Finland
J Biol Chem 279:14746-51. 2004
..It also has no activity with C-6 sugars or sugar alcohols. The K(m) values for l-xylulose and d-ribulose are 9.6 and 4.7 mm, respectively. To our knowledge this is the first report of an NADH-linked l-xylulose reductase...
- Synthesis and evaluation of 1-deoxy-D-xylulose 5-phosphoric acid analogues as alternate substrates for methylerythritol phosphate synthase
David T Fox
Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
J Org Chem 70:1978-85. 2005
..These results are consistent with a retro-aldol/aldol mechanism rather than an alpha-ketol rearrangement for the enzyme-catalyzed conversion of DXP to MEP...
- Pools of 14C-malic acid as a substrate for pyruvate production for the DOXP/MEP pathway of biosynthesis of carotenoids in chloroplasts
V S Saakov
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St Petersburg, 194223 Russia
Dokl Biochem Biophys 400:7-13. 2005
- Early steps of deoxyxylulose phosphate pathway in chromoplasts of higher plants
Monika Fellermeier
, Weinbergweg 22, D-06120 Halle, Germany
Phytochemistry 64:199-207. 2003
....
- Profiling of pentose phosphate pathway intermediates in blood spots by tandem mass spectrometry: application to transaldolase deficiency
Jojanneke H J Huck
Department of Pediatrics, VU University Medical Centre, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands
Clin Chem 49:1375-80. 2003
..Measurements of sugar phosphate intermediates of the PPP may provide new insights into metabolic defects underlying the accumulating polyols...
- Cloning and characterization of the gene encoding phosphoketolase in Leuconostoc mesenteroides isolated from kimchi
Jung Min Lee
School of Agricultural Biotechnology, Seoul National University, Republic of Korea
Biotechnol Lett 27:853-8. 2005
..coli BL21 and E. coli EC101 harboring xfp were 0.28 and 0.14 units/mg, respectively. They both exhibited a 1.5-fold increase in the production of acetic acid from acetyl phosphate compared with their corresponding original strain...
- Synthesis and evaluation of 1-deoxy-D-xylulose 5-phosphate analogues as chelation-based inhibitors of methylerythritol phosphate synthase
Joel R Walker
Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
J Org Chem 70:9955-9. 2005
..The carboxylate (1), methyl ester (3), amide (4), and alcohol (5) analogues were inhibitors with IC50's ranging from 0.25 to 1.0 mM. The hydroxamic acid (2) and amino (6) analogues did not inhibit the enzyme...
- A mutant pyruvate dehydrogenase E1 subunit allows survival of Escherichia coli strains defective in 1-deoxy-D-xylulose 5-phosphate synthase
Susanna Sauret-Güeto
Departament de Bioquimica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona Av Diagonal 645, 08028 Barcelona, Spain
FEBS Lett 580:736-40. 2006
....
- Biochemical and genetic characterization of a novel enzyme of pentitol metabolism: D-arabitol-phosphate dehydrogenase
Mira Povelainen
Danisco Cultor Innovation, Sokeritehtaantie 20, Kantvik 02460, Finland
Biochem J 371:191-7. 2003
..Their apparent biological role is to participate in arabitol catabolism via the 'arabitol phosphate route', similar to the ribitol and xylitol catabolic routes described previously...
- Synthesis and antibacterial activity of mechanism-based inhibitors of KDO8P synthase and DAH7P synthase
Claude Grison
Laboratoire de Chimie Organique Biomoléculaire, UMR CNRS 7565, Institut Nancéien de Chimie Moléculaire, Universite Henri Poincare, Nancy 1, BP 239, F 54506 Vandoeuvre Les Nancy, France
Carbohydr Res 340:529-37. 2005
..The synthesis of new inhibitors closely related to the supposed tetrahedral intermediate substrates for the enzymes is described. The examination of the antibacterial activity of these derivatives is reported...
- Possible direct involvement of the active-site [4Fe-4S] cluster of the GcpE enzyme from Thermus thermophilus in the conversion of MEcPP
Dolapo Adedeji
Department of Chemistry and Biochemistry, Auburn University, 179 Chemistry Building, Auburn, AL 36849, USA
FEBS Lett 581:279-83. 2007
..Similarity of this EPR signal to that detected in ferredoxin:thioredoxin reductase indicates that during the reaction an intermediate is directly bound to the active-site cluster...
- Regulated in vitro synthesis of the enzymes of the deo operon of Escerichia coli. properties of the DNA directed system
B A Svenningsen
Mol Gen Genet 137:289-304. 1975
..The sequential appearance of active enzymes has confirmed the direction of transcription as being dra-tpp-drm-pup and has indicated that the four genes are transcribed into a single tetracistronic message...
- Inhibition of nicotinamide phosphoribosyltransferase by pyridine nucleotides
L S Dietrich
Biochemistry 11:1691-5. 1972
- Rapid and flexible synthesis of 1-deoxy-D-xylulose-5-phosphate, the substrate for 1-deoxy-D-xylulose-5-phosphate reductoisomerase
Russell J Cox
School of Chemistry, University of Bristol, Cantock s Close, Bristol, UK BS8 1TS
Org Biomol Chem 1:3173-7. 2003
..DXP-R was produced by rapid cloning of the dxr gene from Escherichia coli through controlled expression and ion exchange chromatography. The synthetic DXP was fully active in enzyme assays catalysed by recombinant DXP-R...
- A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis
S Takahashi
Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
Proc Natl Acad Sci U S A 95:9879-84. 1998
..coli...
- Transaldolase B of Escherichia coli K-12: cloning of its gene, talB, and characterization of the enzyme from recombinant strains
G A Sprenger
Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Julich, Germany
J Bacteriol 177:5930-6. 1995
..Fructose acted as a C(3) donor at a high apparent K(m) (>/=M) and with a V(rel) of 12%. The enzyme was inhibited by Tris-HCl, phosphate, or sugars with the L configuration at C(2) (L-glyceraldehyde, D-arabinose-5-phosphate)...
- Binding of the natural substrates and products to KDO8P synthase: 31P and 13C solution NMR characterization
Lilia Kaustov
Department of Chemistry, Institute of Catalysis Science and Technology, Technion-Israel Institute of Technology, 32000, Haifa, Israel
Bioorg Chem 31:306-21. 2003
..Since both the substrate A5P and the product KDO8P exhibit multiple anomeric forms in solution, evidence for the preference of recognition and binding of particular forms is sought...
- Activation of a cryptic gene encoding a kinase for L-xylulose opens a new pathway for the utilization of L-lyxose by Escherichia coli
J C Sanchez
Department of Biochemistry, School of Pharmacy, University of Barcelona, Spain
J Biol Chem 269:29665-9. 1994
....
- Construction and characterization of Escherichia coli disruptants defective in the yaeM gene
T Kuzuyama
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
Biosci Biotechnol Biochem 63:776-8. 1999
..This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli...
- Substrate and metal complexes of 3-deoxy-D-manno-octulosonate-8-phosphate synthase from Aquifex aeolicus at 1.9-A resolution. Implications for the condensation mechanism
H S Duewel
The Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA
J Biol Chem 276:8393-402. 2001
..Abstraction of a proton from either of these water molecules by a protein group is expected to elicit a nucleophilic attack of the resulting hydroxide ion on the nearby C-2(PEP), thus triggering the beginning of the catalytic cycle...
- Binding of 5-phospho-D-arabinonohydroxamate and 5-phospho-D-arabinonate inhibitors to zinc phosphomannose isomerase from Candida albicans studied by polarizable molecular mechanics and quantum mechanics
Celine Roux
Laboratoire de Chimie Bioorganique et Bioinorganique, CNRS UMR 8182, Institut de Chimie Moleculaire et des Materiaux d Orsay, Batiment 420, Universite Paris Sud XI, 15 rue Georges Clemenceau, 91405 Orsay, France
J Comput Chem 28:938-57. 2007
....
- 3-Deoxy-D-manno-octulosonate-8-phosphate synthase from Escherichia coli. Model of binding of phosphoenolpyruvate and D-arabinose-5-phosphate
T Wagner
Department of Biology, University of Virginia, Charlottesville, VA, 22903 2477, USA
J Mol Biol 301:233-8. 2000
..Based on the precedent of the d-erythrose-4-phosphate (E4P) modeled in the active site of DAHPS, we have modeled PEP and A5P in KDOPS and compared the coordination of PEP and A5P in KDOPS with that of PEP and E4P in DAHPS...
- Mechanistic studies with 2-C-methyl-D-erythritol 4-phosphate synthase from Escherichia coli
David T Fox
Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
Biochemistry 44:8360-8. 2005
..These observations are consistent with a retro-aldol/aldol mechanism for the rearrangement during conversion of DXP to MEP...
Role of thiamine status and genetic variability in transketolase and other pentose phosphate cycle enzymes in the progression of diabetic nephropathyLukáš Pácal
Department of Pathophysiology, Masaryk University, Brno, Czech Republic
Nephrol Dial Transplant 26:1229-36. 2011
....
- The gene encoding xylulose-5-phosphate/fructose-6-phosphate phosphoketolase (xfp) is conserved among Bifidobacterium species within a more variable region of the genome and both are useful for strain identification
Xianhua Yin
Agriculture and Agri Food Canada, Food Research Program, 93 Stone Road West, Guelph, Ont, Canada N1G 5C9
FEMS Microbiol Lett 246:251-7. 2005
..Oligonucleotide sequences from the conserved and variable xfp regions were used as PCR primers, in combinations of appropriate specificity, for the detection and identification of Bifidobacterium isolates...
- Optimized enzymatic preparation of 1-deoxy-d-xylulose 5-phosphate
Yue Fei Zhou
National Engineering Research Center for Miniaturized Detection Systems, Northwest University, Xi an, Shaanxi, PR China
Bioorg Chem 38:120-3. 2010
..The procedure can also be employed to synthesize isotope labeled 1-deoxy-d-xylulose 5-phosphate by using isotope labeled starting materials...
- The use of (E)- and (Z)-phosphoenol-3-fluoropyruvate as mechanistic probes reveals significant differences between the active sites of KDO8P and DAHP synthases
Cristina M Furdui
Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520 8066, USA
Biochemistry 44:7326-35. 2005
..1 s(-)(1) for (Z)-FPEP, respectively]. The observed data indicate substantial differences in the PEP subsites and open the opportunity for the design of selective inhibitors against these two families of enzymes...
- Conversion of D-ribulose 5-phosphate to D-xylulose 5-phosphate: new insights from structural and biochemical studies on human RPE
Wenguang Liang
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
FASEB J 25:497-504. 2011
..The binary complexes of hRPE reported here will aid in the design of small molecules for modulating the activity of the enzyme and altering flux through the PPP...
- Quantifying the amount and activity of Rubisco in leaves
David S Kubien
Department of Biology, University of New Brunswick, Fredericton, NB, Canada
Methods Mol Biol 684:349-62. 2011
..The required calculations are included. We discuss problems that commonly arise during the extraction and assay of Rubisco...
- Structural and genetic relationships between the O-antigens of Escherichia coli O118 and O151
Bin Liu
TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China
FEMS Immunol Med Microbiol 60:199-207. 2010
..coli O118 and O151. It was suggested that the OGCs of the three bacteria studied originated from a common ancestor...
- Synthesis of arabinitol 1-phosphate and its use for characterization of arabinitol-phosphate dehydrogenase
Nikolai V Soroka
Molecular and Radiation Biology Division, Petersburg Nuclear Physics Institute, Russian Academy of Science, Gatchina 188300, Russia
Carbohydr Res 340:539-46. 2005
..APDH converts Ara-ol1-P to xylulose 5-phosphate in the oxidative reaction; both NAD(+) and NADP(+) were accepted as co-factors. Kinetic parameters for the oxidative and reductive reactions are consistent with a ternary complex mechanism...
- Apicoplast isoprenoid precursor synthesis and the molecular basis of fosmidomycin resistance in Toxoplasma gondii
Sethu C Nair
Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
J Exp Med 208:1547-59. 2011
..We propose that the varied extent of metabolite exchange between host and parasite is a crucial determinator of drug susceptibility and a predictor of future resistance...
- Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains and the biochemical mechanism
H Miyasawa-Hori
Division of Oral Ecology and Biochemistry, Department of Oral Biology, Tohoku University Graduate School of Dentistry, Sendai, Japan
Oral Microbiol Immunol 21:201-5. 2006
....
- On the two components of pyridoxal 5'-phosphate synthase from Bacillus subtilis
Thomas Raschle
ETH Zurich, Institut für Pflanzenwissenschaften, Zurich, Switzerland
J Biol Chem 280:32291-300. 2005
..The results are discussed in light of the nature of the interaction of the two components of the enzyme complex...
- Glucose phosphorylation is required for insulin-dependent mTOR signalling in the heart
Saumya Sharma
Division of Cardiology, The University of Texas Houston Medical School, Houston, Texas 77030, USA
Cardiovasc Res 76:71-80. 2007
..We postulated that mTOR signalling in the heart requires the metabolism of glucose...
- [Synthesis of alpha-D-xylopyranosyl phosphate]
N S Utkina
Bioorg Khim 33:288. 2007
..Synthesis of alpha-D-xylopyranosyl phosphate is carried out by a modified MacDonald method...
- Strain and near attack conformers in enzymic thiamin catalysis: X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor aldo
Peter Asztalos
Institut für Biochemie Biotechnologie, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06120 Halle Saale, Germany
Biochemistry 46:12037-52. 2007
....
- An extended β7α7 substrate-binding loop is essential for efficient catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase
Timothy M Allison
Biomolecular Interaction Centre and Department of Chemistry, University of Canterbury, Christchurch, New Zealand
Biochemistry 50:9318-27. 2011
..Therefore, the presence of the entire extended β7α7 loop is important for efficient catalysis by KDO8P synthase, with the loop acting to promote efficient and productive binding of A5P...
- A novel assay system for the measurement of transketolase activity using xylulokinase from Saccharomyces cerevisiae
Jin Young Lee
Institute of Biotechnological Industry, Inha University, Incheon, 402 751, Korea
Biotechnol Lett 30:899-904. 2008
..The new coupling assay gave reproducible results with E. coli TKT and had a detection limit up to 5 x 10(-4)unit/mg protein. A reliable result was also achieved for the incorporation of XK and TKT into a single reaction...
- The 1.9 A resolution structure of Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a potential drug target
Lena M Henriksson
Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, SE 751 24 Uppsala, Sweden
Acta Crystallogr D Biol Crystallogr 62:807-13. 2006
..Furthermore, the new structure represents an intermediate conformation between the open apo form and the closed holo form observed previously, giving insights into the conformational changes associated with catalysis...
- Preliminary studies on the inhibition of D-sorbitol-6-phosphate 2-dehydrogenase from Escherichia coli with substrate analogues
Celine Roux
Laboratoire de Chimie Bioorganique et Bioinorganique, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8124, Institut de Chimie Moleculaire et des Materiaux d Orsay, Batiment 420, Universite Paris Sud XI, 91405 Orsay Cedex, France
J Enzyme Inhib Med Chem 21:187-92. 2006
..coli D-sorbitol-6-phosphate 2-dehydrogenase yet reported with an IC50 = 7.5 +/- 0.4 microM and corresponding Km/IC50 ratio = about 76...
- D-Ribulose 5-phosphate 3-epimerase: functional and structural relationships to members of the ribulose-phosphate binding (beta/alpha)8-barrel superfamily
Julie Akana
Department of Biochemistry, University of Illinois at Urbana Champaign, 600 S Mathews Avenue, Urbana, Illinois 61801, USA
Biochemistry 45:2493-503. 2006
..Instead, this "superfamily" may result from assembly from smaller modules, including the conserved phosphate binding motif associated with the C-terminal (beta/alpha)(2)-quarter barrel...
- Cytotoxicity of zinc-containing bioactive glasses in contact with human osteoblasts
Valentina Aina
Department of Chemistry IFM and Centre of Excellence NIS, University of Torino, Via Giuria 7, 10125 Torino, Italy
Chem Biol Interact 167:207-18. 2007
..This work highlights the importance of designing Zn-containing bioactive glasses without cytotoxic effects and gives supplementary information about the prooxidant role of zinc in living systems...
- Kinetic and mechanistic analysis of the Escherichia coli ribD-encoded bifunctional deaminase-reductase involved in riboflavin biosynthesis
Maria L B Magalhaes
Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
Biochemistry 47:6499-507. 2008
..coli RibD. On the basis of the observed patterns of inhibition versus the two substrates, we propose that the RibD-catalyzed reduction step follows a kinetic scheme similar to that of its structural homologue, DHFR...
- Structure of L-xylulose-5-Phosphate 3-epimerase (UlaE) from the anaerobic L-ascorbate utilization pathway of Escherichia coli: identification of a novel phosphate binding motif within a TIM barrel fold
Rong Shi
Department of Biochemistry, McGill University, Montreal, Quebec, Canada
J Bacteriol 190:8137-44. 2008
..Mutation and activity measurements for structurally equivalent residues in related epimerases supported this mechanistic proposal...
- Conformational dynamics of the flexible catalytic loop in Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomerase
Sarah L Williams
Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093 0365, USA
Chem Biol Drug Des 73:26-38. 2009
..The substantial fluctuations observed suggest that 1-deoxy-D-xylulose 5-phosphate reductoisomerase may be a promising target for computer-aided drug discovery through the relaxed complex method...
- Mutation in the flexible loop of 1-deoxy-D-xylulose 5-phosphate reductoisomerase broadens substrate utilization
Roberta P M Fernandes
Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, 97331-3507, USA
Arch Biochem Biophys 444:159-64. 2005
..To test this hypothesis, four mutants of the Synechocystis sp. PCC6803 DXR were prepared and a W204F mutant was found to utilize the analog as a substrate...
- Structure activity and molecular modeling analyses of ribose- and base-modified uridine 5'-triphosphate analogues at the human P2Y2 and P2Y4 receptors
Kenneth A Jacobson
Molecular Recognition Section, Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Biochem Pharmacol 71:540-9. 2006
....
- Relationship of the oxidative pentose shunt pathway to lipid synthesis in Drosophila melanogaster
B W Geer
Biochem Genet 17:881-95. 1979
..Environmental temperature influences on the fatty acid composition of larvae are not altered by an oxidative pentose shunt deficiency...
- Reduction precedes cytidylyl transfer without substrate channeling in distinct active sites of the bifunctional CDP-ribitol synthase from Haemophilus influenzae
M Zolli
Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
Biochemistry 40:5041-8. 2001
....
- Characterization of the D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase gene (xfp) from Bifidobacterium lactis
L Meile
Laboratory of Food Microbiology, Institute of Food Science, ETH Zurich, CH 8092 Zurich, Switzerland
J Bacteriol 183:2929-36. 2001
..However, xfp is transcribed in B. lactis as a monocistronic operon. It is the first reported and sequenced gene of a phosphoketolase...
- Examination of donor substrate conversion in yeast transketolase
E Fiedler
Institute of Biochemistry, Department of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, 06120 Halle/Saale, Kurt-Mothes-Strabetae 3, Germany
J Biol Chem 276:16051-8. 2001
..A., Schörken, U., Sprenger, G., and Sahm, H. (1995) Eur. J. Biochem. 230, 525-532)...
- Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production
S W Kim
Marine Bioproducts Engineering Center, Department of Chemical Engineering, University of California, Berkeley, CA 94720 1462, USA
Biotechnol Bioeng 72:408-15. 2001
..A comparison of the three E. coli strains transformed with the arabinose-inducible dxs on a medium-copy plasmid revealed that lycopene production was highest in XL1-Blue...
- Functional and biochemical characterization of a recombinant 3-Deoxy-D-manno-octulosonic acid 8-phosphate synthase from the hyperthermophilic bacterium Aquifex aeolicus
H S Duewel
Interdepartmental Program in Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan, 48109 1065, USA
Biochem Biophys Res Commun 263:346-51. 1999
..5 h (90 degrees C), 8.1 h (80 degrees C), and 30.3 h (70 degrees C). KdsA appeared to follow Michaelis-Menton kinetics with K(A5-P)(m) = 8 - 74 microM, K(PEP)(m) = 43-28 microM, and k(cat) = 0.4-2.0 s(-1) between 60 and 90 degrees C...
- The localisation of 2-carboxy-D-arabinitol 1-phosphate and inhibition of Rubisco in leaves of Phaseolus vulgaris L
M A Parry
Biochemistry and Physiology Department, IACR Rothamsted, Harpenden, UK
FEBS Lett 444:106-10. 1999
..Furthermore, CA1P is shown to be in the chloroplasts of mesophyll cells. Thus, CA1P does play a physiological role in the regulation of Rubisco...
- Haemophilus influenzae type b-specific antibody in infants after maternal immunization
J A Englund
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA
Pediatr Infect Dis J 16:1122-30. 1997
..To study the kinetics of Haemophilus influenzae type b (Hib)-specific antibody in infants born to mothers immunized with an Hib polysaccharide or one of two Hib conjugate vaccines...
- Pentose phosphate pathway mutants of yeast
Z Lobo
Mol Gen Genet 185:367-8. 1982
..A lesion in the latter enzyme alone leaves growth practically unaffected. The mutations define the respective structural genes...
- Deoxyribonucleoside catabolism and thymine incorporation in mutants of Escherichia coli lacking deoxyriboaldolase
A Munch-Petersen
Eur J Biochem 15:191-202. 1970
- Ribulose diphosphate carboxylase from autotrophic microorganisms
B A McFadden
J Bacteriol 110:633-42. 1972
..Ribulose diphosphate carboxylase in extracts of H. eutropha, H. facilis, Chromatium D, Rhodospirillum rubrum, and Chlorella pyrenoidosa were also inhibited to varying extents by cyanide and antiserum to the H. eutropha enzyme...
- Mutants constitutive for nucleoside-catabolizing enzymes in Escherichia coli K12. Isolation, charactrization and mapping
A Munch-Petersen
Eur J Biochem 27:208-15. 1972
- Glucose metabolism in 6 phosphogluconolactonase mutants of Escherichia coli
S R Kupor
J Biol Chem 247:1904-10. 1972
- Studies on the quaternary structure of the first enzyme for histidine biosynthesis
A R Tebar
Experientia 29:1477-9. 1973
- Regulation of purine synthesis de novo in human fibroblasts by purine nucleotides and phosphoribosylpyrophosphate
M A Becker
Department of Medicine, University of Chicago Pritzker School of Medicine, Illinois 60637
J Biol Chem 262:14531-7. 1987
..3) Activation of amido PRT by PP-Rib-P is nearly maximal at base line in fibroblasts with regulatory defects in PP-Rib-P synthetase...
- Two mechanisms for growth inhibition by elevated transport of sugar phosphates in Escherichia coli
R J Kadner
Department of Microbiology, School of Medicine, University of Virginia, Charlottesville 22908
J Gen Microbiol 138:2007-14. 1992
..It is likely that the control of uhpT transcription by catabolite repression exists to limit the level of UhpT transport activity and thereby prevent the toxic events that result from elevated uptake of its substrates...
- Regulation of energy metabolism in macrophages during hypoxia. Roles of fructose 2,6-bisphosphate and ribose 1,5-bisphosphate
T Kawaguchi
Department of Biochemistry, Dallas Veterans Affairs Medical Center, Dallas, Texas 75216, USA
J Biol Chem 276:28554-61. 2001
..These data suggest that the activation of the above enzymes was mediated by protein kinase C acting via activation of phosphatidylinositol specific phospholipase C in the macrophages during hypoxia...
- Expression of the xylulose 5-phosphate phosphoketolase gene, xpkA, from Lactobacillus pentosus MD363 is induced by sugars that are fermented via the phosphoketolase pathway and is repressed by glucose mediated by CcpA and the mannose phosphoenolpyruvate p
Clara C Posthuma
SILS, BioCentrum, University of Amsterdam, 1018 TV Amsterdam, The Netherlands
Appl Environ Microbiol 68:831-7. 2002
....
- Crystal structure of activated ribulose-1,5-bisphosphate carboxylase/oxygenase from green alga Chlamydomonas reinhardtii complexed with 2-carboxyarabinitol-1,5-bisphosphate
Eiichi Mizohata
Department of Materials Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan
J Mol Biol 316:679-91. 2002
..Furthermore, the unusual electron density of residue 471 of the L-subunit, which has been deduced to be threonine from the genomic DNA sequence, suggests that the residue is isoleucine produced by RNA editing or O(gamma)-methylthreonine...
- Role of the non-mevalonate pathway in indole alkaloid production by Catharanthus roseus hairy roots
Seung-Beom Hong
Department of Biochemistry and Cell Biology, Rice University, MS-140, 6100 Main St, Houston, Texas 77005, USA
Biotechnol Prog 19:1105-8. 2003
..These results suggest that the DXP pathway is a major provider of carbon for the monoterpenoid pathway leading to the formation of indole alkaloids in C. roseus hairy roots in the exponential phase...
- Xylitol inhibition of anaerobic acid production by Streptococcus mutans at various pH levels
H Miyasawa
Department of Lifelong Oral Health Sciences, Tohoku University Graduate School of Dentistry, Sendai, Japan
Oral Microbiol Immunol 18:215-9. 2003
..mutans more effectively...
- Structure of an effector-induced inactivated state of ribulose 1,5-bisphosphate carboxylase/oxygenase: the binary complex between enzyme and xylulose 1,5-bisphosphate
J Newman
Department of Molecular Biology, Biomedical Centre, Uppsala, Sweden
Structure 2:495-502. 1994
..We have used one of these, xylulose 1,5-bisphosphate as an analogue of the natural substrate and co-crystallized it with the enzyme...
- Structural evidence for a hydride transfer mechanism of catalysis in phosphoglucose isomerase from Pyrococcus furiosus
Michael K Swan
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA
J Biol Chem 278:47261-8. 2003
....
- Alternative pathway for the formation of 4,5-dihydroxy-2,3-pentanedione, the proposed precursor of 4-hydroxy-5-methyl-3(2H)-furanone as well as autoinducer-2, and its detection as natural constituent of tomato fruit
Tobias Hauck
, , Am Hubland, , Germany
Biochim Biophys Acta 1623:109-19. 2003
..Application of o-phenylenediamine into ripe tomatoes led to the detection of 3-(1,2-dihydroxyethyl)-2-methylquinoxaline by means of HPLC-MS/MS analysis implying the genuine occurrence of 4,5-dihydroxy-2,3-pentanedione in this fruit...
- The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli
Uwe Sauer
Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
J Biol Chem 279:6613-9. 2004
..metabolism with an extraordinary flexibility to cope with varying catabolic and anabolic demands, which raises two general questions: why do only a few bacteria contain both isoforms, and how do other organisms manage NADPH metabolism?..
- Isoprenoid biosynthesis via the MEP pathway. Synthesis of (3,4)-3,4-dihydroxy-5-oxohexylphosphonic acid, an isosteric analogue of 1-deoxy-D-xylulose 5-phosphate, the substrate of the 1-deoxy-D-xylulose 5-phosphate reducto-isomerase
Odile Meyer
Universite Louis Pasteur, CNRS UMR 7123, Institut Le Bel, 4 rue Blaise Pascal, 67070 Strasbourg Cedex, France
Org Biomol Chem 1:4367-72. 2003
..The enzyme was, however, less efficient with the methylene phosphonate analogue than with the natural substrate...
- Bifunctional catalysis by CDP-ribitol synthase: convergent recruitment of reductase and cytidylyltransferase activities in Haemophilus influenzae and Staphylococcus aureus
Mark P Pereira
Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
Biochemistry 43:11802-12. 2004
....
- Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway
Peter Richard
VTT Biotechnology, P O Box 1500, Tietotie 2, Espoo 02044 VTT, Finland
FEMS Yeast Res 3:185-9. 2003
..Under anaerobic conditions ethanol is produced from L-arabinose, but at a very low rate. The reasons for the low rate of L-arabinose fermentation are discussed...
- Identification and quantitation of phosphorus metabolites in yeast neutral pH extracts by nuclear magnetic resonance spectroscopy
A Teleman
VTT Chemical Technology, Espoo, Finland
Anal Biochem 272:71-9. 1999
..No such accumulation occurred when the cells were grown in yeast-peptone-dextrose medium. Trimetaphosphate (intracellular concentration about 0.2 mM) was detected in both cold methanol-chloroform and perchloric acid extracts...
- Optimal growth and ethanol production from xylose by recombinant Saccharomyces cerevisiae require moderate D-xylulokinase activity
Yong-Su Jin
Department of Food Science, University of Wisconsin, Madison, Wisconsin 53706, USA
Appl Environ Microbiol 69:495-503. 2003
..The selected transformant had approximately four copies of XYL3 per haploid genome and had moderate XK activity. It converted xylose into ethanol efficiently...
- Escherichia coli phnN, encoding ribose 1,5-bisphosphokinase activity (phosphoribosyl diphosphate forming): dual role in phosphonate degradation and NAD biosynthesis pathways
Bjarne Hove-Jensen
Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, Copenhagen, Denmark
J Bacteriol 185:2793-801. 2003
....
- Effect of bivalent cations on the interaction of transketolase with its donor substrate
Irina A Sevostyanova
A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow 119992, Russia
Proteins 71:541-5. 2008
..In particular, this becomes apparent on binding of xylulose 5-phosphates to one of the two active centers of the enzyme, which caused the second center to undergo a several fold decrease in the affinity for the donor substrate...
- Analysis of the plastidic phosphate translocator gene family in Arabidopsis and identification of new phosphate translocator-homologous transporters, classified by their putative substrate-binding site
Silke Knappe
, Lehrstuhl II, Gyrhofstrasse 15, D-50931 Cologne, Germany
Plant Physiol 131:1178-90. 2003
....
- Mutants of Escherichia coli unable to metabolize cytidine: isolation and characterization
K Hammer-Jespersen
Mol Gen Genet 126:177-86. 1973
- Inhibition mode of a bisubstrate inhibitor of KDO8P synthase: a frequency-selective REDOR solid-state and solution NMR characterization
Lilia Kaustov
Department of Chemistry and Institute of Catalysis Science and Technology, Technion-Israel Institute of Technology, Haifa 32000, Israel
J Am Chem Soc 125:4662-9. 2003
....
