pentosephosphates

Summary

Top Publications

  1. doi A raison d'être for two distinct pathways in the early steps of plant isoprenoid biosynthesis?
    Andrea Hemmerlin
    Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, IBMP CNRS UPR2357, Universite de Strasbourg, 28 rue Goethe, F 67083 Strasbourg cedex, France
    Prog Lipid Res 51:95-148. 2012
  2. pmc Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol
    G A Sprenger
    Institut für Biotechnologie 1 des Forschungszentrums Jülich, Germany
    Proc Natl Acad Sci U S A 94:12857-62. 1997
  3. pmc A new family of enzymes catalyzing the first committed step of the methylerythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in bacteria
    Felix J Sangari
    Departamento de Biologia Molecular, Universidad de Cantabria and Instituto de Biomedicina y Biotecnología de Cantabria IBBTEC, CSIC UC IDICAN, 39011 Santander, Spain
    Proc Natl Acad Sci U S A 107:14081-6. 2010
  4. pmc A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis
    S Takahashi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 95:9879-84. 1998
  5. pmc Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis
    L M Lois
    Departament de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, Marti i Franques 1, 08028 Barcelona, Spain
    Proc Natl Acad Sci U S A 95:2105-10. 1998
  6. ncbi A mutant pyruvate dehydrogenase E1 subunit allows survival of Escherichia coli strains defective in 1-deoxy-D-xylulose 5-phosphate synthase
    Susanna Sauret-Güeto
    Departament de Bioquimica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona Av Diagonal 645, 08028 Barcelona, Spain
    FEBS Lett 580:736-40. 2006
  7. pmc Isoprenoid biosynthesis in Synechocystis sp. strain PCC6803 is stimulated by compounds of the pentose phosphate cycle but not by pyruvate or deoxyxylulose-5-phosphate
    Yuri V Ershov
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA
    J Bacteriol 184:5045-51. 2002
  8. ncbi Structural basis of fosmidomycin action revealed by the complex with 2-C-methyl-D-erythritol 4-phosphate synthase (IspC). Implications for the catalytic mechanism and anti-malaria drug development
    Stefan Steinbacher
    Max Planck Institut fur Biochemie, Abteilung für Strukturforschung, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Biol Chem 278:18401-7. 2003
  9. ncbi Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugs
    H Jomaa
    Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, Friedrichstrasse 24, D 35392 Giessen, Germany
    Science 285:1573-6. 1999
  10. ncbi Chlorophyta exclusively use the 1-deoxyxylulose 5-phosphate/2-C-methylerythritol 4-phosphate pathway for the biosynthesis of isoprenoids
    J Schwender
    Botanisches Institut, Lehrstuhl II, , Germany
    Planta 212:416-23. 2001

Scientific Experts

Detail Information

Publications115 found, 100 shown here

  1. doi A raison d'être for two distinct pathways in the early steps of plant isoprenoid biosynthesis?
    Andrea Hemmerlin
    Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, IBMP CNRS UPR2357, Universite de Strasbourg, 28 rue Goethe, F 67083 Strasbourg cedex, France
    Prog Lipid Res 51:95-148. 2012
    ..This latter aspect still requires more work and present conclusions are preliminary, although some general features seem to exist...
  2. pmc Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol
    G A Sprenger
    Institut für Biotechnologie 1 des Forschungszentrums Jülich, Germany
    Proc Natl Acad Sci U S A 94:12857-62. 1997
    ....
  3. pmc A new family of enzymes catalyzing the first committed step of the methylerythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in bacteria
    Felix J Sangari
    Departamento de Biologia Molecular, Universidad de Cantabria and Instituto de Biomedicina y Biotecnología de Cantabria IBBTEC, CSIC UC IDICAN, 39011 Santander, Spain
    Proc Natl Acad Sci U S A 107:14081-6. 2010
    ....
  4. pmc A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis
    S Takahashi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 95:9879-84. 1998
    ..coli...
  5. pmc Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis
    L M Lois
    Departament de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, Marti i Franques 1, 08028 Barcelona, Spain
    Proc Natl Acad Sci U S A 95:2105-10. 1998
    ..D-1-Deoxyxylulose-5-phosphate synthase belongs to a family of transketolase-like proteins that are highly conserved in evolution...
  6. ncbi A mutant pyruvate dehydrogenase E1 subunit allows survival of Escherichia coli strains defective in 1-deoxy-D-xylulose 5-phosphate synthase
    Susanna Sauret-Güeto
    Departament de Bioquimica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona Av Diagonal 645, 08028 Barcelona, Spain
    FEBS Lett 580:736-40. 2006
    ....
  7. pmc Isoprenoid biosynthesis in Synechocystis sp. strain PCC6803 is stimulated by compounds of the pentose phosphate cycle but not by pyruvate or deoxyxylulose-5-phosphate
    Yuri V Ershov
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA
    J Bacteriol 184:5045-51. 2002
    ..strain PCC6803. Our data support alternative routes of entry of pentose phosphate cycle substrates derived from photosynthesis...
  8. ncbi Structural basis of fosmidomycin action revealed by the complex with 2-C-methyl-D-erythritol 4-phosphate synthase (IspC). Implications for the catalytic mechanism and anti-malaria drug development
    Stefan Steinbacher
    Max Planck Institut fur Biochemie, Abteilung für Strukturforschung, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Biol Chem 278:18401-7. 2003
    ..Both sites are connected by a spacer of three methylene groups. The substrate molecule, 1-d-deoxyxylulose 5-phosphate, can be superimposed onto fosmidomycin, explaining the stereochemical course of the reaction...
  9. ncbi Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugs
    H Jomaa
    Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, Friedrichstrasse 24, D 35392 Giessen, Germany
    Science 285:1573-6. 1999
    ..Both drugs suppressed the in vitro growth of multidrug-resistant P. falciparum strains. After therapy with these drugs, mice infected with the rodent malaria parasite P. vinckei were cured...
  10. ncbi Chlorophyta exclusively use the 1-deoxyxylulose 5-phosphate/2-C-methylerythritol 4-phosphate pathway for the biosynthesis of isoprenoids
    J Schwender
    Botanisches Institut, Lehrstuhl II, , Germany
    Planta 212:416-23. 2001
    ....
  11. pmc 1-Deoxy-D-xylulose 5-phosphate reductoisomerase (IspC) from Mycobacterium tuberculosis: towards understanding mycobacterial resistance to fosmidomycin
    Rakesh K Dhiman
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Bacteriol 187:8395-402. 2005
    ..Thus, M. tuberculosis resistance to fosmidomycin is not due to intrinsic properties of Rv2870c, and the enzyme appears to be a valid drug target in this pathogen...
  12. ncbi Nudix hydrolases that degrade dinucleoside and diphosphoinositol polyphosphates also have 5-phosphoribosyl 1-pyrophosphate (PRPP) pyrophosphatase activity that generates the glycolytic activator ribose 1,5-bisphosphate
    David I Fisher
    Cell Regulation and Signalling Group, School of Biological Sciences, University of Liverpool, Liverpool L69 7ZB, United Kingdom
    J Biol Chem 277:47313-7. 2002
    ..radiodurans DR2356 enzyme and for the DIPP subfamily. In the latter case, generation of the glycolytic activator ribose 1,5-bisphosphate may be a new function for these enzymes...
  13. ncbi Multistate binding in pyridoxine 5'-phosphate synthase: 1.96 A crystal structure in complex with 1-deoxy-D-xylulose phosphate
    Joanne I Yeh
    Molecular Biology, Cell Biology, and Biochemistry Department, Brown University, Box G J2, Providence, RI 02912 9108, USA
    Biochemistry 41:11649-57. 2002
    ....
  14. ncbi Isoprenoid biosynthesis via the methylerythritol phosphate pathway. Mechanistic investigations of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase
    Jean François Hoeffler
    Université Louis Pasteur CNRS, Institut Le Bel, Strasbourg, France
    Eur J Biochem 269:4446-57. 2002
    ..The equilibrium was, however, largely displaced in favour of the formation of MEP. The reduction step required the presence of a divalent cation such as Mg(2+) or Mn(2+)...
  15. pmc The metabolic significance of octulose phosphates in the photosynthetic carbon reduction cycle in spinach
    John F Williams
    Research School of Chemistry, Australian National University, Canberra, A C T, 0200, Australia
    Photosynth Res 90:125-48. 2006
    ....
  16. ncbi The methylerythritol phosphate pathway is functionally active in all intraerythrocytic stages of Plasmodium falciparum
    Maria B Cassera
    Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo, 05508 900 Sao Paulo, Brazil
    J Biol Chem 279:51749-59. 2004
    ..falciparum, and de novo biosynthesis of pyridoxal in a protozoan is reported. Its absence in the human host makes both pathways very attractive as potential new targets for antimalarial drug development...
  17. ncbi Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase complexed with cofactors: implications of a flexible loop movement upon substrate binding
    Shunsuke Yajima
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    J Biochem 131:313-7. 2002
    ..A flexible loop covering the substrate binding site plays an important role in the enzymatic reaction and the determination of substrate specificity...
  18. ncbi Possible direct involvement of the active-site [4Fe-4S] cluster of the GcpE enzyme from Thermus thermophilus in the conversion of MEcPP
    Dolapo Adedeji
    Department of Chemistry and Biochemistry, Auburn University, 179 Chemistry Building, Auburn, AL 36849, USA
    FEBS Lett 581:279-83. 2007
    ..Similarity of this EPR signal to that detected in ferredoxin:thioredoxin reductase indicates that during the reaction an intermediate is directly bound to the active-site cluster...
  19. ncbi Binding of 5-phospho-D-arabinonohydroxamate and 5-phospho-D-arabinonate inhibitors to zinc phosphomannose isomerase from Candida albicans studied by polarizable molecular mechanics and quantum mechanics
    Celine Roux
    Laboratoire de Chimie Bioorganique et Bioinorganique, CNRS UMR 8182, Institut de Chimie Moleculaire et des Materiaux d Orsay, Batiment 420, Universite Paris Sud XI, 15 rue Georges Clemenceau, 91405 Orsay, France
    J Comput Chem 28:938-57. 2007
    ....
  20. ncbi E. coli MEP synthase: steady-state kinetic analysis and substrate binding
    Andrew T Koppisch
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 41:236-43. 2002
    ..Fosmidomycin was an uncompetitive inhibitor against NADPH and gave a pattern representative of slow, tight-binding competitive inhibition against DXP. These results are consistent with an ordered mechanism where NADPH binds before DXP...
  21. ncbi Function of His185 in Aquifex aeolicus 3-deoxy-D-manno-octulosonate 8-phosphate synthase
    Jian Wang
    Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Mol Biol 324:205-14. 2002
    ..aeolicus KDO8PS is to orient PEP in the active site of the enzyme in such a way that a water molecule on the sinister (si) side of PEP can be activated by direct coordination to the metal ion...
  22. pmc Biochemical and genetic characterization of a novel enzyme of pentitol metabolism: D-arabitol-phosphate dehydrogenase
    Mira Povelainen
    Danisco Cultor Innovation, Sokeritehtaantie 20, Kantvik 02460, Finland
    Biochem J 371:191-7. 2003
    ..Their apparent biological role is to participate in arabitol catabolism via the 'arabitol phosphate route', similar to the ribitol and xylitol catabolic routes described previously...
  23. ncbi A novel NADH-linked l-xylulose reductase in the l-arabinose catabolic pathway of yeast
    Ritva Verho
    VTT Biotechnology, P O Box 1500, FIN 02044 VTT, Finland
    J Biol Chem 279:14746-51. 2004
    ..It also has no activity with C-6 sugars or sugar alcohols. The K(m) values for l-xylulose and d-ribulose are 9.6 and 4.7 mm, respectively. To our knowledge this is the first report of an NADH-linked l-xylulose reductase...
  24. ncbi Cloning and characterization of the gene encoding phosphoketolase in Leuconostoc mesenteroides isolated from kimchi
    Jung Min Lee
    School of Agricultural Biotechnology, Seoul National University, Republic of Korea
    Biotechnol Lett 27:853-8. 2005
    ..coli BL21 and E. coli EC101 harboring xfp were 0.28 and 0.14 units/mg, respectively. They both exhibited a 1.5-fold increase in the production of acetic acid from acetyl phosphate compared with their corresponding original strain...
  25. ncbi Rapid and flexible synthesis of 1-deoxy-D-xylulose-5-phosphate, the substrate for 1-deoxy-D-xylulose-5-phosphate reductoisomerase
    Russell J Cox
    School of Chemistry, University of Bristol, Cantock s Close, Bristol, UK BS8 1TS
    Org Biomol Chem 1:3173-7. 2003
    ..DXP-R was produced by rapid cloning of the dxr gene from Escherichia coli through controlled expression and ion exchange chromatography. The synthetic DXP was fully active in enzyme assays catalysed by recombinant DXP-R...
  26. ncbi Synthesis and evaluation of 1-deoxy-D-xylulose 5-phosphate analogues as chelation-based inhibitors of methylerythritol phosphate synthase
    Joel R Walker
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    J Org Chem 70:9955-9. 2005
    ..The carboxylate (1), methyl ester (3), amide (4), and alcohol (5) analogues were inhibitors with IC50's ranging from 0.25 to 1.0 mM. The hydroxamic acid (2) and amino (6) analogues did not inhibit the enzyme...
  27. ncbi Early steps of deoxyxylulose phosphate pathway in chromoplasts of higher plants
    Monika Fellermeier
    Biozentrum der Universität Halle, Weinbergweg 22, D 06120 Halle, Germany
    Phytochemistry 64:199-207. 2003
    ....
  28. ncbi Binding of the natural substrates and products to KDO8P synthase: 31P and 13C solution NMR characterization
    Lilia Kaustov
    Department of Chemistry, Institute of Catalysis Science and Technology, Technion Israel Institute of Technology, 32000, Haifa, Israel
    Bioorg Chem 31:306-21. 2003
    ..Since both the substrate A5P and the product KDO8P exhibit multiple anomeric forms in solution, evidence for the preference of recognition and binding of particular forms is sought...
  29. ncbi Two methods for determination of transketolase activity
    I A Sevostyanova
    Belozersky Institute of Physico Chemical Biology, Lomonosov Moscow State University, Moscow, Russia
    Biochemistry (Mosc) 71:560-2. 2006
    ..It is not needed in the second method. The range of transketolase concentration in the activity assay is 0.036-0.144 U/ml for the first method and 1.8-6.8 U/ml for the second one...
  30. pmc Catalytic by-product formation and ligand binding by ribulose bisphosphate carboxylases from different phylogenies
    F Grant Pearce
    School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8020, New Zealand
    Biochem J 399:525-34. 2006
    ..Our observations also illustrate the phylogenetic diversity of mechanisms for regulating Rubisco and raise questions about whether an activase-like mechanism should be expected outside the green-algal/higher-plant lineage...
  31. ncbi Detection of novel enzyme intermediates in PEP-utilizing enzymes
    Karen S Anderson
    Department of Pharmacology, SHM B350B, Yale University School of Medicine, 333 Cedar Street New Haven, CT 06520, USA
    Arch Biochem Biophys 433:47-58. 2005
    ..A novel application of this approach using time-resolved electrospray mass spectrometry to detect chemically labile enzyme intermediates is also discussed...
  32. ncbi Synthesis and antibacterial activity of mechanism-based inhibitors of KDO8P synthase and DAH7P synthase
    Claude Grison
    Laboratoire de Chimie Organique Biomoléculaire, UMR CNRS 7565, Institut Nancéien de Chimie Moléculaire, Universite Henri Poincare, Nancy 1, BP 239, F 54506 Vandoeuvre Les Nancy, France
    Carbohydr Res 340:529-37. 2005
    ..The synthesis of new inhibitors closely related to the supposed tetrahedral intermediate substrates for the enzymes is described. The examination of the antibacterial activity of these derivatives is reported...
  33. ncbi Synthesis and evaluation of 1-deoxy-D-xylulose 5-phosphoric acid analogues as alternate substrates for methylerythritol phosphate synthase
    David T Fox
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    J Org Chem 70:1978-85. 2005
    ..These results are consistent with a retro-aldol/aldol mechanism rather than an alpha-ketol rearrangement for the enzyme-catalyzed conversion of DXP to MEP...
  34. ncbi Structures of Aquifex aeolicus KDO8P synthase in complex with R5P and PEP, and with a bisubstrate inhibitor: role of active site water in catalysis
    J Wang
    Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Biochemistry 40:15676-83. 2001
    ..Overall, the process can be described as a syn addition of water and A5P to the si side of PEP...
  35. ncbi Probing catalysis by Escherichia coli dTDP-glucose-4,6-dehydratase: identification and preliminary characterization of functional amino acid residues at the active site
    A D Hegeman
    Department of Biochemistry, University of Wisconsin Madison, 53705, USA
    Biochemistry 40:6598-610. 2001
    ..Additional steady-state techniques were employed in an attempt to further limit the assignment of rate limitation. The results are discussed within the context of the 4,6-dehydratase active site model and chemical mechanism...
  36. pmc Transaldolase B of Escherichia coli K-12: cloning of its gene, talB, and characterization of the enzyme from recombinant strains
    G A Sprenger
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Julich, Germany
    J Bacteriol 177:5930-6. 1995
    ..Fructose acted as a C(3) donor at a high apparent K(m) (>/=M) and with a V(rel) of 12%. The enzyme was inhibited by Tris-HCl, phosphate, or sugars with the L configuration at C(2) (L-glyceraldehyde, D-arabinose-5-phosphate)...
  37. ncbi Regulated in vitro synthesis of the enzymes of the deo operon of Escerichia coli. properties of the DNA directed system
    B A Svenningsen
    Mol Gen Genet 137:289-304. 1975
    ..The sequential appearance of active enzymes has confirmed the direction of transcription as being dra-tpp-drm-pup and has indicated that the four genes are transcribed into a single tetracistronic message...
  38. ncbi Activation of a cryptic gene encoding a kinase for L-xylulose opens a new pathway for the utilization of L-lyxose by Escherichia coli
    J C Sanchez
    Department of Biochemistry, School of Pharmacy, University of Barcelona, Spain
    J Biol Chem 269:29665-9. 1994
    ....
  39. ncbi Inhibition of nicotinamide phosphoribosyltransferase by pyridine nucleotides
    L S Dietrich
    Biochemistry 11:1691-5. 1972
  40. ncbi Pools of 14C-malic acid as a substrate for pyruvate production for the DOXP/MEP pathway of biosynthesis of carotenoids in chloroplasts
    V S Saakov
    Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St Petersburg, 194223 Russia
    Dokl Biochem Biophys 400:7-13. 2005
  41. ncbi Substrate and metal complexes of 3-deoxy-D-manno-octulosonate-8-phosphate synthase from Aquifex aeolicus at 1.9-A resolution. Implications for the condensation mechanism
    H S Duewel
    The Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 276:8393-402. 2001
    ..Abstraction of a proton from either of these water molecules by a protein group is expected to elicit a nucleophilic attack of the resulting hydroxide ion on the nearby C-2(PEP), thus triggering the beginning of the catalytic cycle...
  42. ncbi 3-Deoxy-D-manno-octulosonate-8-phosphate synthase from Escherichia coli. Model of binding of phosphoenolpyruvate and D-arabinose-5-phosphate
    T Wagner
    Department of Biology, University of Virginia, Charlottesville, VA, 22903 2477, USA
    J Mol Biol 301:233-8. 2000
    ..Based on the precedent of the d-erythrose-4-phosphate (E4P) modeled in the active site of DAHPS, we have modeled PEP and A5P in KDOPS and compared the coordination of PEP and A5P in KDOPS with that of PEP and E4P in DAHPS...
  43. ncbi Profiling of pentose phosphate pathway intermediates in blood spots by tandem mass spectrometry: application to transaldolase deficiency
    Jojanneke H J Huck
    Department of Pediatrics, VU University Medical Centre, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands
    Clin Chem 49:1375-80. 2003
    ..We hypothesized that they are derived from sugar phosphate intermediates of the pentose phosphate pathway (PPP), and we developed a semiquantitative method for profiling of pentose phosphate pathway intermediates...
  44. ncbi Construction and characterization of Escherichia coli disruptants defective in the yaeM gene
    T Kuzuyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    Biosci Biotechnol Biochem 63:776-8. 1999
    ..This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli...
  45. pmc The multiple activities of Escherichia coli endonuclease IV and the extreme lability of 5'-terminal base-free deoxyribose 5-phosphates
    V Bailly
    Biochimie, Faculte des Sciences, Universite de Liege, Belgium
    Biochem J 259:761-8. 1989
    ..The 3'-phosphatase activity of E. coli endonuclease VI/exonuclease III and E. coli endonuclease IV can also be useful when the AP site has been excised by a beta delta-elimination reaction...
  46. pmc Dynamic, ligand-dependent conformational change triggers reaction of ribose-1,5-bisphosphate isomerase from Thermococcus kodakarensis KOD1
    Akira Nakamura
    Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    J Biol Chem 287:20784-96. 2012
    ..The structural features of the substrate-binding form and biochemical evidence lead us to propose that the isomerase reaction proceeds via a cis-phosphoenolate intermediate...
  47. doi Mechanistic insights into 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a key enzyme of the MEP terpenoid biosynthetic pathway
    Heng Li
    Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education, College of Life Sciences, Northwest University, Xi an, China
    FEBS J 280:5896-905. 2013
    ....
  48. pmc Potent inhibition of the C-P lyase nucleosidase PhnI by Immucillin-A triphosphate
    Siddhesh S Kamat
    Department of Chemistry, Texas A and M University, College Station, Texas 77840, United States
    Biochemistry 52:7366-8. 2013
    ..Here we describe the synthesis of Immucillin-A triphosphate, a mimic of the transition state structure for the nucleosidase reaction catalyzed by PhnI. This compound inhibits PhnI with a dissociation constant of 20 nM at pH 7.5. ..
  49. pmc Apicoplast isoprenoid precursor synthesis and the molecular basis of fosmidomycin resistance in Toxoplasma gondii
    Sethu C Nair
    Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    J Exp Med 208:1547-59. 2011
    ..We propose that the varied extent of metabolite exchange between host and parasite is a crucial determinator of drug susceptibility and a predictor of future resistance...
  50. ncbi Mutation in the flexible loop of 1-deoxy-D-xylulose 5-phosphate reductoisomerase broadens substrate utilization
    Roberta P M Fernandes
    Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, 97331 3507, USA
    Arch Biochem Biophys 444:159-64. 2005
    ..To test this hypothesis, four mutants of the Synechocystis sp. PCC6803 DXR were prepared and a W204F mutant was found to utilize the analog as a substrate...
  51. doi Quantifying the amount and activity of Rubisco in leaves
    David S Kubien
    Department of Biology, University of New Brunswick, Fredericton, NB, Canada
    Methods Mol Biol 684:349-62. 2011
    ..The required calculations are included. We discuss problems that commonly arise during the extraction and assay of Rubisco...
  52. ncbi Structural and genetic relationships between the O-antigens of Escherichia coli O118 and O151
    Bin Liu
    TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China
    FEMS Immunol Med Microbiol 60:199-207. 2010
    ..coli O118 and O151. It was suggested that the OGCs of the three bacteria studied originated from a common ancestor...
  53. doi An extended β7α7 substrate-binding loop is essential for efficient catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase
    Timothy M Allison
    Biomolecular Interaction Centre and Department of Chemistry, University of Canterbury, Christchurch, New Zealand
    Biochemistry 50:9318-27. 2011
    ..Therefore, the presence of the entire extended β7α7 loop is important for efficient catalysis by KDO8P synthase, with the loop acting to promote efficient and productive binding of A5P...
  54. ncbi Mechanistic studies with 2-C-methyl-D-erythritol 4-phosphate synthase from Escherichia coli
    David T Fox
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 44:8360-8. 2005
    ..These observations are consistent with a retro-aldol/aldol mechanism for the rearrangement during conversion of DXP to MEP...
  55. ncbi The gene encoding xylulose-5-phosphate/fructose-6-phosphate phosphoketolase (xfp) is conserved among Bifidobacterium species within a more variable region of the genome and both are useful for strain identification
    Xianhua Yin
    Agriculture and Agri Food Canada, Food Research Program, 93 Stone Road West, Guelph, Ont, Canada N1G 5C9
    FEMS Microbiol Lett 246:251-7. 2005
    ..Oligonucleotide sequences from the conserved and variable xfp regions were used as PCR primers, in combinations of appropriate specificity, for the detection and identification of Bifidobacterium isolates...
  56. ncbi The use of (E)- and (Z)-phosphoenol-3-fluoropyruvate as mechanistic probes reveals significant differences between the active sites of KDO8P and DAHP synthases
    Cristina M Furdui
    Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520 8066, USA
    Biochemistry 44:7326-35. 2005
    ..1 s(-)(1) for (Z)-FPEP, respectively]. The observed data indicate substantial differences in the PEP subsites and open the opportunity for the design of selective inhibitors against these two families of enzymes...
  57. doi Conversion of D-ribulose 5-phosphate to D-xylulose 5-phosphate: new insights from structural and biochemical studies on human RPE
    Wenguang Liang
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    FASEB J 25:497-504. 2011
    ..The binary complexes of hRPE reported here will aid in the design of small molecules for modulating the activity of the enzyme and altering flux through the PPP...
  58. doi Role of thiamine status and genetic variability in transketolase and other pentose phosphate cycle enzymes in the progression of diabetic nephropathy
    Lukáš Pácal
    Department of Pathophysiology, Masaryk University, Brno, Czech Republic
    Nephrol Dial Transplant 26:1229-36. 2011
    ....
  59. ncbi [Synthesis of alpha-D-xylopyranosyl phosphate]
    S D Mal'tsev
    Bioorg Khim 33:288. 2007
    ..Synthesis of alpha-D-xylopyranosyl phosphate is carried out by a modified MacDonald method...
  60. pmc Glucose phosphorylation is required for insulin-dependent mTOR signalling in the heart
    Saumya Sharma
    Division of Cardiology, The University of Texas Houston Medical School, Houston, Texas 77030, USA
    Cardiovasc Res 76:71-80. 2007
    ..We postulated that mTOR signalling in the heart requires the metabolism of glucose...
  61. ncbi Strain and near attack conformers in enzymic thiamin catalysis: X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor aldo
    Peter Asztalos
    Institut für Biochemie Biotechnologie, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06120 Halle Saale, Germany
    Biochemistry 46:12037-52. 2007
    ....
  62. ncbi A novel assay system for the measurement of transketolase activity using xylulokinase from Saccharomyces cerevisiae
    Jin Young Lee
    Institute of Biotechnological Industry, Inha University, Incheon, 402 751, Korea
    Biotechnol Lett 30:899-904. 2008
    ..The new coupling assay gave reproducible results with E. coli TKT and had a detection limit up to 5 x 10(-4)unit/mg protein. A reliable result was also achieved for the incorporation of XK and TKT into a single reaction...
  63. ncbi Kinetic and mechanistic analysis of the Escherichia coli ribD-encoded bifunctional deaminase-reductase involved in riboflavin biosynthesis
    Maria L B Magalhaes
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 47:6499-507. 2008
    ..coli RibD. On the basis of the observed patterns of inhibition versus the two substrates, we propose that the RibD-catalyzed reduction step follows a kinetic scheme similar to that of its structural homologue, DHFR...
  64. pmc Structure of L-xylulose-5-Phosphate 3-epimerase (UlaE) from the anaerobic L-ascorbate utilization pathway of Escherichia coli: identification of a novel phosphate binding motif within a TIM barrel fold
    Rong Shi
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    J Bacteriol 190:8137-44. 2008
    ..Mutation and activity measurements for structurally equivalent residues in related epimerases supported this mechanistic proposal...
  65. pmc Conformational dynamics of the flexible catalytic loop in Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomerase
    Sarah L Williams
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093 0365, USA
    Chem Biol Drug Des 73:26-38. 2009
    ..The substantial fluctuations observed suggest that 1-deoxy-D-xylulose 5-phosphate reductoisomerase may be a promising target for computer-aided drug discovery through the relaxed complex method...
  66. ncbi Optimized enzymatic preparation of 1-deoxy-d-xylulose 5-phosphate
    Yue Fei Zhou
    National Engineering Research Center for Miniaturized Detection Systems, Northwest University, Xi an, Shaanxi, PR China
    Bioorg Chem 38:120-3. 2010
    ..The procedure can also be employed to synthesize isotope labeled 1-deoxy-d-xylulose 5-phosphate by using isotope labeled starting materials...
  67. ncbi Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains and the biochemical mechanism
    H Miyasawa-Hori
    Division of Oral Ecology and Biochemistry, Department of Oral Biology, Tohoku University Graduate School of Dentistry, Sendai, Japan
    Oral Microbiol Immunol 21:201-5. 2006
    ....
  68. ncbi Preliminary studies on the inhibition of D-sorbitol-6-phosphate 2-dehydrogenase from Escherichia coli with substrate analogues
    Celine Roux
    Laboratoire de Chimie Bioorganique et Bioinorganique, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8124, Institut de Chimie Moleculaire et des Materiaux d Orsay, Batiment 420, Universite Paris Sud XI, 91405 Orsay Cedex, France
    J Enzyme Inhib Med Chem 21:187-92. 2006
    ..coli D-sorbitol-6-phosphate 2-dehydrogenase yet reported with an IC50 = 7.5 +/- 0.4 microM and corresponding Km/IC50 ratio = about 76...
  69. ncbi The 1.9 A resolution structure of Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a potential drug target
    Lena M Henriksson
    Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, SE 751 24 Uppsala, Sweden
    Acta Crystallogr D Biol Crystallogr 62:807-13. 2006
    ..Furthermore, the new structure represents an intermediate conformation between the open apo form and the closed holo form observed previously, giving insights into the conformational changes associated with catalysis...
  70. ncbi D-Ribulose 5-phosphate 3-epimerase: functional and structural relationships to members of the ribulose-phosphate binding (beta/alpha)8-barrel superfamily
    Julie Akana
    Department of Biochemistry, University of Illinois at Urbana Champaign, 600 S Mathews Avenue, Urbana, Illinois 61801, USA
    Biochemistry 45:2493-503. 2006
    ..Instead, this "superfamily" may result from assembly from smaller modules, including the conserved phosphate binding motif associated with the C-terminal (beta/alpha)(2)-quarter barrel...
  71. ncbi Cytotoxicity of zinc-containing bioactive glasses in contact with human osteoblasts
    Valentina Aina
    Department of Chemistry IFM and Centre of Excellence NIS, University of Torino, Via Giuria 7, 10125 Torino, Italy
    Chem Biol Interact 167:207-18. 2007
    ..This work highlights the importance of designing Zn-containing bioactive glasses without cytotoxic effects and gives supplementary information about the prooxidant role of zinc in living systems...
  72. ncbi Structure activity and molecular modeling analyses of ribose- and base-modified uridine 5'-triphosphate analogues at the human P2Y2 and P2Y4 receptors
    Kenneth A Jacobson
    Molecular Recognition Section, Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Biochem Pharmacol 71:540-9. 2006
    ....
  73. ncbi On the two components of pyridoxal 5'-phosphate synthase from Bacillus subtilis
    Thomas Raschle
    ETH Zurich, Institut für Pflanzenwissenschaften, Zurich, Switzerland
    J Biol Chem 280:32291-300. 2005
    ..The results are discussed in light of the nature of the interaction of the two components of the enzyme complex...
  74. ncbi Relationship of the oxidative pentose shunt pathway to lipid synthesis in Drosophila melanogaster
    B W Geer
    Biochem Genet 17:881-95. 1979
    ..Environmental temperature influences on the fatty acid composition of larvae are not altered by an oxidative pentose shunt deficiency...
  75. ncbi Regulation of energy metabolism in macrophages during hypoxia. Roles of fructose 2,6-bisphosphate and ribose 1,5-bisphosphate
    T Kawaguchi
    Department of Biochemistry, Dallas Veterans Affairs Medical Center, Dallas, Texas 75216, USA
    J Biol Chem 276:28554-61. 2001
    ..These data suggest that the activation of the above enzymes was mediated by protein kinase C acting via activation of phosphatidylinositol specific phospholipase C in the macrophages during hypoxia...
  76. ncbi Reduction precedes cytidylyl transfer without substrate channeling in distinct active sites of the bifunctional CDP-ribitol synthase from Haemophilus influenzae
    M Zolli
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
    Biochemistry 40:5041-8. 2001
    ....
  77. ncbi Glucose metabolism in 6 phosphogluconolactonase mutants of Escherichia coli
    S R Kupor
    J Biol Chem 247:1904-10. 1972
  78. ncbi Examination of donor substrate conversion in yeast transketolase
    E Fiedler
    Institute of Biochemistry, Department of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, 06120 Halle/Saale, Kurt-Mothes-Strabetae 3, Germany
    J Biol Chem 276:16051-8. 2001
    ..A., Schörken, U., Sprenger, G., and Sahm, H. (1995) Eur. J. Biochem. 230, 525-532)...
  79. ncbi Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production
    S W Kim
    Marine Bioproducts Engineering Center, Department of Chemical Engineering, University of California, Berkeley, CA 94720 1462, USA
    Biotechnol Bioeng 72:408-15. 2001
    ..A comparison of the three E. coli strains transformed with the arabinose-inducible dxs on a medium-copy plasmid revealed that lycopene production was highest in XL1-Blue...
  80. ncbi Functional and biochemical characterization of a recombinant 3-Deoxy-D-manno-octulosonic acid 8-phosphate synthase from the hyperthermophilic bacterium Aquifex aeolicus
    H S Duewel
    Interdepartmental Program in Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan, 48109 1065, USA
    Biochem Biophys Res Commun 263:346-51. 1999
    ..5 h (90 degrees C), 8.1 h (80 degrees C), and 30.3 h (70 degrees C). KdsA appeared to follow Michaelis-Menton kinetics with K(A5-P)(m) = 8 - 74 microM, K(PEP)(m) = 43-28 microM, and k(cat) = 0.4-2.0 s(-1) between 60 and 90 degrees C...
  81. ncbi Identification and quantitation of phosphorus metabolites in yeast neutral pH extracts by nuclear magnetic resonance spectroscopy
    A Teleman
    VTT Chemical Technology, Espoo, Finland
    Anal Biochem 272:71-9. 1999
    ..No such accumulation occurred when the cells were grown in yeast-peptone-dextrose medium. Trimetaphosphate (intracellular concentration about 0.2 mM) was detected in both cold methanol-chloroform and perchloric acid extracts...
  82. ncbi The localisation of 2-carboxy-D-arabinitol 1-phosphate and inhibition of Rubisco in leaves of Phaseolus vulgaris L
    M A Parry
    Biochemistry and Physiology Department, IACR Rothamsted, Harpenden, UK
    FEBS Lett 444:106-10. 1999
    ..Furthermore, CA1P is shown to be in the chloroplasts of mesophyll cells. Thus, CA1P does play a physiological role in the regulation of Rubisco...
  83. ncbi Haemophilus influenzae type b-specific antibody in infants after maternal immunization
    J A Englund
    Department of Microbiology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA
    Pediatr Infect Dis J 16:1122-30. 1997
    ..To study the kinetics of Haemophilus influenzae type b (Hib)-specific antibody in infants born to mothers immunized with an Hib polysaccharide or one of two Hib conjugate vaccines...
  84. ncbi Pentose phosphate pathway mutants of yeast
    Z Lobo
    Mol Gen Genet 185:367-8. 1982
    ..A lesion in the latter enzyme alone leaves growth practically unaffected. The mutations define the respective structural genes...
  85. ncbi Deoxyribonucleoside catabolism and thymine incorporation in mutants of Escherichia coli lacking deoxyriboaldolase
    A Munch-Petersen
    Eur J Biochem 15:191-202. 1970
  86. pmc Ribulose diphosphate carboxylase from autotrophic microorganisms
    B A McFadden
    J Bacteriol 110:633-42. 1972
    ..Ribulose diphosphate carboxylase in extracts of H. eutropha, H. facilis, Chromatium D, Rhodospirillum rubrum, and Chlorella pyrenoidosa were also inhibited to varying extents by cyanide and antiserum to the H. eutropha enzyme...
  87. ncbi Mutants constitutive for nucleoside-catabolizing enzymes in Escherichia coli K12. Isolation, charactrization and mapping
    A Munch-Petersen
    Eur J Biochem 27:208-15. 1972
  88. ncbi Studies on the quaternary structure of the first enzyme for histidine biosynthesis
    A R Tebar
    Experientia 29:1477-9. 1973
  89. ncbi Regulation of purine synthesis de novo in human fibroblasts by purine nucleotides and phosphoribosylpyrophosphate
    M A Becker
    Department of Medicine, University of Chicago Pritzker School of Medicine, Illinois 60637
    J Biol Chem 262:14531-7. 1987
    ..3) Activation of amido PRT by PP-Rib-P is nearly maximal at base line in fibroblasts with regulatory defects in PP-Rib-P synthetase...
  90. ncbi Two mechanisms for growth inhibition by elevated transport of sugar phosphates in Escherichia coli
    R J Kadner
    Department of Microbiology, School of Medicine, University of Virginia, Charlottesville 22908
    J Gen Microbiol 138:2007-14. 1992
    ..It is likely that the control of uhpT transcription by catabolite repression exists to limit the level of UhpT transport activity and thereby prevent the toxic events that result from elevated uptake of its substrates...
  91. pmc Expression of the xylulose 5-phosphate phosphoketolase gene, xpkA, from Lactobacillus pentosus MD363 is induced by sugars that are fermented via the phosphoketolase pathway and is repressed by glucose mediated by CcpA and the mannose phosphoenolpyruvate p
    Clara C Posthuma
    SILS, BioCentrum, University of Amsterdam, 1018 TV Amsterdam, The Netherlands
    Appl Environ Microbiol 68:831-7. 2002
    ....
  92. ncbi Crystal structure of activated ribulose-1,5-bisphosphate carboxylase/oxygenase from green alga Chlamydomonas reinhardtii complexed with 2-carboxyarabinitol-1,5-bisphosphate
    Eiichi Mizohata
    Department of Materials Chemistry, Graduate School of Engineering, Osaka University, 2 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Mol Biol 316:679-91. 2002
    ..Furthermore, the unusual electron density of residue 471 of the L-subunit, which has been deduced to be threonine from the genomic DNA sequence, suggests that the residue is isoleucine produced by RNA editing or O(gamma)-methylthreonine...
  93. ncbi Bifunctional catalysis by CDP-ribitol synthase: convergent recruitment of reductase and cytidylyltransferase activities in Haemophilus influenzae and Staphylococcus aureus
    Mark P Pereira
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
    Biochemistry 43:11802-12. 2004
    ....
  94. ncbi Isoprenoid biosynthesis via the MEP pathway. Synthesis of (3,4)-3,4-dihydroxy-5-oxohexylphosphonic acid, an isosteric analogue of 1-deoxy-D-xylulose 5-phosphate, the substrate of the 1-deoxy-D-xylulose 5-phosphate reducto-isomerase
    Odile Meyer
    Universite Louis Pasteur, CNRS UMR 7123, Institut Le Bel, 4 rue Blaise Pascal, 67070 Strasbourg Cedex, France
    Org Biomol Chem 1:4367-72. 2003
    ..The enzyme was, however, less efficient with the methylene phosphonate analogue than with the natural substrate...
  95. ncbi The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli
    Uwe Sauer
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    J Biol Chem 279:6613-9. 2004
    ..metabolism with an extraordinary flexibility to cope with varying catabolic and anabolic demands, which raises two general questions: why do only a few bacteria contain both isoforms, and how do other organisms manage NADPH metabolism?..
  96. ncbi Alternative pathway for the formation of 4,5-dihydroxy-2,3-pentanedione, the proposed precursor of 4-hydroxy-5-methyl-3(2H)-furanone as well as autoinducer-2, and its detection as natural constituent of tomato fruit
    Tobias Hauck
    Lehrstuhl fur Lebensmittelchemie, Universitat Wurzburg, Am Hubland, 97074 Wurzburg, Germany
    Biochim Biophys Acta 1623:109-19. 2003
    ..Application of o-phenylenediamine into ripe tomatoes led to the detection of 3-(1,2-dihydroxyethyl)-2-methylquinoxaline by means of HPLC-MS/MS analysis implying the genuine occurrence of 4,5-dihydroxy-2,3-pentanedione in this fruit...
  97. ncbi Structural evidence for a hydride transfer mechanism of catalysis in phosphoglucose isomerase from Pyrococcus furiosus
    Michael K Swan
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA
    J Biol Chem 278:47261-8. 2003
    ....
  98. ncbi Structure of an effector-induced inactivated state of ribulose 1,5-bisphosphate carboxylase/oxygenase: the binary complex between enzyme and xylulose 1,5-bisphosphate
    J Newman
    Department of Molecular Biology, Biomedical Centre, Uppsala, Sweden
    Structure 2:495-502. 1994
    ..We have used one of these, xylulose 1,5-bisphosphate as an analogue of the natural substrate and co-crystallized it with the enzyme...
  99. ncbi Xylitol inhibition of anaerobic acid production by Streptococcus mutans at various pH levels
    H Miyasawa
    Department of Lifelong Oral Health Sciences, Tohoku University Graduate School of Dentistry, Sendai, Japan
    Oral Microbiol Immunol 18:215-9. 2003
    ..mutans more effectively...
  100. ncbi Role of the non-mevalonate pathway in indole alkaloid production by Catharanthus roseus hairy roots
    Seung Beom Hong
    Department of Biochemistry and Cell Biology, Rice University, MS 140, 6100 Main St, Houston, Texas 77005, USA
    Biotechnol Prog 19:1105-8. 2003
    ..These results suggest that the DXP pathway is a major provider of carbon for the monoterpenoid pathway leading to the formation of indole alkaloids in C. roseus hairy roots in the exponential phase...
  101. ncbi Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway
    Peter Richard
    VTT Biotechnology, P O Box 1500, Tietotie 2, Espoo 02044 VTT, Finland
    FEMS Yeast Res 3:185-9. 2003
    ..Under anaerobic conditions ethanol is produced from L-arabinose, but at a very low rate. The reasons for the low rate of L-arabinose fermentation are discussed...

Research Grants51

  1. Exploring Novel Targeting Strategies for AIDS Protozoal Pathogens
    Karen S Anderson; Fiscal Year: 2011
    ..Unfortunately, successful combination therapies against the HIV-1 virus still leave most patients susceptible to opportunistic parasitic infections and new therapies are desperately needed. ..
  2. Novel Approach to Study Transient Enzyme Intermediates
    Karen Anderson; Fiscal Year: 2008
    ..These studies will aid in pioneering a powerful and novel analytical methodology for detecting, characterizing and quantitating enzyme intermediates on a rapid time scale. ..
  3. Universal Technology for Profiling the Dynamics of Normal & Oncogenic Signaling
    Karen Anderson; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  4. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2007
    ..This project will use X-ray crystallography to provide such information for a number of key proteins in N. gonorrhoeae that are involved in cell wall synthesis. ..
  5. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 2007
    ..abstract_text> ..
  6. Novel Approach to Study Transient Enzyme Intermediates
    Karen Anderson; Fiscal Year: 2007
    ..These studies will aid in pioneering a powerful and novel analytical methodology for detecting, characterizing and quantitating enzyme intermediates on a rapid time scale. ..
  7. A Molecular Approach For Opportunistic HIV-1 Infections
    Karen Anderson; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  8. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 2005
    ..abstract_text> ..
  9. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 2006
    ..abstract_text> ..
  10. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2006
    ..These studies will provide a framework for future studies aimed at structure-based drug design and will provide substantial insight into the mechanisms of peptidoglycan synthesis. [unreadable] [unreadable]..
  11. Novel Approach to Study Transient Enzyme Intermediates
    Karen Anderson; Fiscal Year: 2006
    ..These studies will aid in pioneering a powerful and novel analytical methodology for detecting, characterizing and quantitating enzyme intermediates on a rapid time scale. ..
  12. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2008
    ..This project will use X-ray crystallography to provide such information for a number of key proteins in N. gonorrhoeae that are involved in cell wall synthesis. ..
  13. Molecular Mechanisms of EGF Signaling & Iressa/Tarceva Inhibition in NSCLC
    Karen Anderson; Fiscal Year: 2008
    ..The studies described in this application are designed help unravel these molecular details to allow us to differentiate cancer cells from normal cells and assess the usefulness of current and new cancer therapies. ..
  14. Universal Technology for Profiling the Dynamics of Normal & Oncogenic Signaling
    Karen Anderson; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  15. Molecular Mechanisms of EGF Signaling & Iressa/Tarceva Inhibition in NSCLC
    Karen S Anderson; Fiscal Year: 2011
    ..The studies described in this application are designed help unravel these molecular details to allow us to differentiate cancer cells from normal cells and assess the usefulness of current and new cancer therapies. ..
  16. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2009
    ..This project will use X-ray crystallography to provide such information for a number of key proteins in N. gonorrhoeae that are involved in cell wall synthesis. ..
  17. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen S Anderson; Fiscal Year: 2010
    ..The studies outlined in this proposal will combine mechanistic studies with computational guidance to design more effective HIV therapies that have improved therapeutic properties. ..
  18. Exploring Novel Targeting Strategies for AIDS Protozoal Pathogens
    Karen S Anderson; Fiscal Year: 2010
    ..Unfortunately, successful combination therapies against the HIV-1 virus still leave most patients susceptible to opportunistic parasitic infections and new therapies are desperately needed. ..
  19. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2010
    ..This project will use X-ray crystallography to provide such information for a number of key proteins in N. gonorrhoeae that are involved in cell wall synthesis. ..
  20. Molecular Mechanisms of EGF Signaling & Iressa/Tarceva Inhibition in NSCLC
    Karen S Anderson; Fiscal Year: 2010
    ..The studies described in this application are designed help unravel these molecular details to allow us to differentiate cancer cells from normal cells and assess the usefulness of current and new cancer therapies. ..
  21. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 2009
    ..The studies outlined in this proposal will combine mechanistic studies with computational guidance to design more effective HIV therapies that have improved therapeutic properties. ..
  22. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2009
    ..This project will use X-ray crystallography to provide such information for a number of key proteins in N. gonorrhoeae that are involved in cell wall synthesis. ..
  23. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 2008
    ..The studies outlined in this proposal will combine mechanistic studies with computational guidance to design more effective HIV therapies that have improved therapeutic properties. ..
  24. Molecular Mechanisms of EGF Signaling & Iressa/Tarceva Inhibition in NSCLC
    Karen Anderson; Fiscal Year: 2009
    ..The studies described in this application are designed help unravel these molecular details to allow us to differentiate cancer cells from normal cells and assess the usefulness of current and new cancer therapies. ..
  25. A Molecular Approach For Opportunistic HIV-1 Infections
    Karen Anderson; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  26. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 2005
    ..abstract_text> ..
  27. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2005
    ..These studies will provide a framework for future studies aimed at structure-based drug design and will provide substantial insight into the mechanisms of peptidoglycan synthesis. ..
  28. MOLECULAR APPROACH FOR OPPORTUNISTIC HIV INFECTIONS
    Karen Anderson; Fiscal Year: 2000
    ..abstract_text> ..
  29. MECHANISTIC STUDIES ON PEP UTILIZING ENZYMES
    Karen Anderson; Fiscal Year: 2000
    ..In addition as the investigator develops the quantitative aspects of the rapid mixing, pulsed-flow ESI-MS technique further, she will extend the studies of these enzymes to determine the full kinetic profile. ..
  30. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 2000
    ..4) Design and Evaluate Novel Bifunctional HIV-1 Inhibitors created by linking nucleoside and non-nucleoside analogs. ..
  31. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 1993
    ..5. Explore the basis for resistance to AZT and non-nucleoside analogs by providing a complete kinetic characterization of mutant reverse transcriptases...
  32. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 1999
    ..3) Mechanistic studies will be conducted on the inhibition of mitochondrial gamma polymerase by nucleoside analogues which may be important to understand cytotoxicity. ..
  33. MOLECULAR APPROACH FOR OPPORTUNISTIC HIV INFECTIONS
    Karen Anderson; Fiscal Year: 1999
    ..abstract_text> ..
  34. MOLECULAR APPROACH FOR OPPORTUNISTIC HIV INFECTIONS
    Karen Anderson; Fiscal Year: 2001
    ..abstract_text> ..
  35. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 2001
    ..4) Design and Evaluate Novel Bifunctional HIV-1 Inhibitors created by linking nucleoside and non-nucleoside analogs. ..
  36. MECHANISTIC STUDIES ON PEP UTILIZING ENZYMES
    Karen Anderson; Fiscal Year: 2001
    ..In addition as the investigator develops the quantitative aspects of the rapid mixing, pulsed-flow ESI-MS technique further, she will extend the studies of these enzymes to determine the full kinetic profile. ..
  37. A Molecular Approach For Opportunistic HIV-1 Infections
    Karen Anderson; Fiscal Year: 2005
    ....
  38. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 2004
    ..abstract_text> ..
  39. A Molecular Approach For Opportunistic HIV-1 Infections
    Karen Anderson; Fiscal Year: 2004
    ....
  40. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2004
    ..These studies will provide a framework for future studies aimed at structure-based drug design and will provide substantial insight into the mechanisms of peptidoglycan synthesis. ..
  41. MECHANISTIC STUDIES ON PEP UTILIZING ENZYMES
    Karen Anderson; Fiscal Year: 2003
    ..In addition as the investigator develops the quantitative aspects of the rapid mixing, pulsed-flow ESI-MS technique further, she will extend the studies of these enzymes to determine the full kinetic profile. ..
  42. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 2003
    ..4) Design and Evaluate Novel Bifunctional HIV-1 Inhibitors created by linking nucleoside and non-nucleoside analogs. ..
  43. Molecular Targets in Peptidoglycan Synthesis
    Christopher Davies; Fiscal Year: 2003
    ..These studies will provide a framework for future studies aimed at structure-based drug design and will provide substantial insight into the mechanisms of peptidoglycan synthesis. ..
  44. MECHANISTIC STUDIES ON PEP UTILIZING ENZYMES
    Karen Anderson; Fiscal Year: 2002
    ..In addition as the investigator develops the quantitative aspects of the rapid mixing, pulsed-flow ESI-MS technique further, she will extend the studies of these enzymes to determine the full kinetic profile. ..
  45. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 2002
    ..4) Design and Evaluate Novel Bifunctional HIV-1 Inhibitors created by linking nucleoside and non-nucleoside analogs. ..
  46. MECHANISM AND INHIBITION OF HIV REVERSE TRANSCRIPTASE
    Karen Anderson; Fiscal Year: 2001
    ..4) Design and Evaluate Novel Bifunctional HIV-1 Inhibitors created by linking nucleoside and non-nucleoside analogs. ..