fructosephosphates

Summary

Top Publications

  1. ncbi The crystal and solution studies of glucosamine-6-phosphate synthase from Candida albicans
    Joanna Raczynska
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, ul Noskowskiego 12 14, 61 704 Poznan, Poland
    J Mol Biol 372:672-88. 2007
  2. ncbi Structure and catalytic mechanism of glucosamine 6-phosphate deaminase from Escherichia coli at 2.1 A resolution
    G Oliva
    Instituto de Fisica de Sao Carlos, Universidade de Sao Paulo, Brazil
    Structure 3:1323-32. 1995
  3. ncbi Accumulation of glucose 6-phosphate or fructose 6-phosphate is responsible for destabilization of glucose transporter mRNA in Escherichia coli
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    J Biol Chem 278:15608-14. 2003
  4. ncbi Crystal structure of the hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3): a possible new target for cancer therapy
    Song Gun Kim
    Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA
    J Biol Chem 281:2939-44. 2006
  5. ncbi A direct substrate-substrate interaction found in the kinase domain of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
    Song Gun Kim
    Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    J Mol Biol 370:14-26. 2007
  6. ncbi Involvement of the C terminus in intramolecular nitrogen channeling in glucosamine 6-phosphate synthase: evidence from a 1.6 A crystal structure of the isomerase domain
    A Teplyakov
    European Molecular Biology Laboratory, Germany
    Structure 6:1047-55. 1998
  7. ncbi Identification of fructose 6-phosphate- and fructose 1-phosphate-binding residues in the regulatory protein of glucokinase
    Maria Veiga-da-Cunha
    Laboratory of Physiological Chemistry, University of Louvain and Christian de Duve Institute of Cellular Pathology, B 1200 Brussels, Belgium
    J Biol Chem 277:8466-73. 2002
  8. pmc Differences in regulatory properties between human and rat glucokinase regulatory protein
    Katy J Brocklehurst
    Cardiovascular and Gastrointestinal Department, AstraZeneca, Macclesfield, Cheshire SK10 4TG, UK
    Biochem J 378:693-7. 2004
  9. ncbi Enzymic activities in two populations of purified rat islet beta-cells
    A Sener
    Laboratory of Experimental Medicine, Brussels Free University, B-1070 Brussels, Belgium
    Int J Mol Med 8:285-9. 2001
  10. ncbi Purification and kinetic properties of 6-phosphofructo-1-kinase from gilthead sea bream muscle
    Dominica Mediavilla
    Departament de Bioquimica i Biologia Molecular, Facultat de Farmacia, Universitat de Barcelona, Diagonal 643, 08028 Barcelona, Spain
    Biochim Biophys Acta 1770:706-15. 2007

Research Grants

  1. STRUCTURE OF ORAL BACTERIAL ADHESINS
    Teresa Ruiz; Fiscal Year: 2008

Scientific Experts

Detail Information

Publications132 found, 100 shown here

  1. ncbi The crystal and solution studies of glucosamine-6-phosphate synthase from Candida albicans
    Joanna Raczynska
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, ul Noskowskiego 12 14, 61 704 Poznan, Poland
    J Mol Biol 372:672-88. 2007
    ..This study of Gfa1p focuses on the features that distinguish it from the prokaryotic homologue in terms of quaternary structure, control of the enzymatic activity and details of the isomerase active site...
  2. ncbi Structure and catalytic mechanism of glucosamine 6-phosphate deaminase from Escherichia coli at 2.1 A resolution
    G Oliva
    Instituto de Fisica de Sao Carlos, Universidade de Sao Paulo, Brazil
    Structure 3:1323-32. 1995
    ..The determination of the structure of this protein provides fundamental knowledge for understanding its mode of action and the nature of allosteric conformational changes that regulate its function...
  3. ncbi Accumulation of glucose 6-phosphate or fructose 6-phosphate is responsible for destabilization of glucose transporter mRNA in Escherichia coli
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    J Biol Chem 278:15608-14. 2003
    ..We conclude that the RNase E-dependent destabilization of ptsG mRNA occurs in response to accumulation of glucose-6-P or fructose-6-P...
  4. ncbi Crystal structure of the hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3): a possible new target for cancer therapy
    Song Gun Kim
    Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA
    J Biol Chem 281:2939-44. 2006
    ..This finding suggests that a molecule in which EDTA is covalently linked to ADP is a good starting molecule for the development of new cancer-therapeutic molecules...
  5. ncbi A direct substrate-substrate interaction found in the kinase domain of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
    Song Gun Kim
    Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    J Mol Biol 370:14-26. 2007
    ..The site-directed mutant study and inhibition kinetics suggest that this reaction will be catalyzed most efficiently by the protein when the substrates bind to the active pocket in an ordered manner in which ATP binds first...
  6. ncbi Involvement of the C terminus in intramolecular nitrogen channeling in glucosamine 6-phosphate synthase: evidence from a 1.6 A crystal structure of the isomerase domain
    A Teplyakov
    European Molecular Biology Laboratory, Germany
    Structure 6:1047-55. 1998
    ..Knowledge of the three-dimensional structure of GlmS is essential for understanding the general principles of catalysis by ketol isomerases and the mechanism of nitrogen transfer in glutamine amidotransferases...
  7. ncbi Identification of fructose 6-phosphate- and fructose 1-phosphate-binding residues in the regulatory protein of glucokinase
    Maria Veiga-da-Cunha
    Laboratory of Physiological Chemistry, University of Louvain and Christian de Duve Institute of Cellular Pathology, B 1200 Brussels, Belgium
    J Biol Chem 277:8466-73. 2002
    ..These results are consistent with GKRP having one single binding site for phosphate esters. They also show that a missense mutation of GKRP can lead to a gain of function...
  8. pmc Differences in regulatory properties between human and rat glucokinase regulatory protein
    Katy J Brocklehurst
    Cardiovascular and Gastrointestinal Department, AstraZeneca, Macclesfield, Cheshire SK10 4TG, UK
    Biochem J 378:693-7. 2004
    ..Residues that are not conserved between the rodent and human proteins affect both the affinity for fructose 6-phosphate and sorbitol 6-phosphate and the inhibitory potency of GKRP on glucokinase in the absence of these ligands...
  9. ncbi Enzymic activities in two populations of purified rat islet beta-cells
    A Sener
    Laboratory of Experimental Medicine, Brussels Free University, B-1070 Brussels, Belgium
    Int J Mol Med 8:285-9. 2001
    ..These findings thus argue against a low expression of either low-Km hexokinase(s) or lactate dehydrogenase as major determinants of the glucose-sensing device in beta-cells...
  10. ncbi Purification and kinetic properties of 6-phosphofructo-1-kinase from gilthead sea bream muscle
    Dominica Mediavilla
    Departament de Bioquimica i Biologia Molecular, Facultat de Farmacia, Universitat de Barcelona, Diagonal 643, 08028 Barcelona, Spain
    Biochim Biophys Acta 1770:706-15. 2007
    ..Unlike the mammalian muscle enzyme, our findings support that phosphorylation of PFKM may exert a major role during starvation in fish muscle...
  11. ncbi A deeper investigation on carbohydrate cycling in Sinorhizobium meliloti
    I Gosselin
    , UMR CNRS 6022, , , 33 rue Saint Leu, 80039 Cedex, Amiens, France
    FEBS Lett 499:45-9. 2001
    ..Furthermore, carbohydrate cycling in S. meliloti is also observed on fructose, making the situation in this bacterium significantly different from that depicted for alginate-synthesizing species...
  12. ncbi Creation of an allosteric phosphofructokinase starting with a nonallosteric enzyme. The case of dictyostelium discoideum phosphofructokinase
    Belen Santamaria
    Departamento de Bioquímica and Instituto de Investigaciones Biomédicas Alberto Sols UAM CSIC, Facultad de Medicina de la Universidad Autonoma, 28029 Madrid, Spain
    J Biol Chem 277:1210-6. 2002
    ....
  13. ncbi MgATP-dependent activation by phosphoenolpyruvate of the E187A mutant of Escherichia coli phosphofructokinase
    A S Pham
    Division of Pathology and Laboratory Medicine, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Biochemistry 40:4150-8. 2001
    ..The present study examines the role of Glu 187 at the allosteric site in the binding of Fru-6-P and offers a more complex explanation of the mechanism than that described by traditional allosteric mechanistic models...
  14. ncbi Expression of key substrate cycle enzymes in rat spermatogenic cells: fructose 1,6 bisphosphatase and 6 phosphofructose 1-kinase
    Alejandro J Yañez
    Instituto de Bioquimica, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile
    J Cell Physiol 212:807-16. 2007
    ....
  15. ncbi Profiling of pentose phosphate pathway intermediates in blood spots by tandem mass spectrometry: application to transaldolase deficiency
    Jojanneke H J Huck
    Department of Pediatrics, VU University Medical Centre, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands
    Clin Chem 49:1375-80. 2003
    ..We hypothesized that they are derived from sugar phosphate intermediates of the pentose phosphate pathway (PPP), and we developed a semiquantitative method for profiling of pentose phosphate pathway intermediates...
  16. ncbi Excess mannose limits the growth of phosphomannose isomerase PMI40 deletion strain of Saccharomyces cerevisiae
    Juha Pekka Pitkänen
    MediCel Ltd, Haartmaninkatu 8, FIN 00290 Helsinki, Finland
    J Biol Chem 279:55737-43. 2004
    ....
  17. ncbi Quantitative estimation of channeling from early glycolytic intermediates to CO in intact Escherichia coli
    Georgia Shearer
    Department of Biology, Washington University, St Louis, MO 63130, USA
    FEBS J 272:3260-9. 2005
    ..The data also suggest that, in addition to pathway enzymes being associated with one another, some are free in the aqueous cytosol. How sensitive the degree of channeling is to growth or experimental conditions remains to be determined...
  18. ncbi The structures of inhibitor complexes of Pyrococcus furiosus phosphoglucose isomerase provide insights into substrate binding and catalysis
    John M Berrisford
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    J Mol Biol 343:649-57. 2004
    ..These similarities suggest that aspects of the catalytic mechanisms of these two structurally unrelated PGIs may be similar and based on an enediol intermediate...
  19. ncbi Development of a quantification system of ionic dissociative metabolites using an FT-IR/ATR method
    T Pan
    Department of Sustainable Resource Sciences, Faculty of Bioresources, Mie University, 1515 Kamihama cho, Tsu, Mie 514 8507, Japan
    Bioprocess Biosyst Eng 26:133-9. 2003
    ..In addition, the stability of quantification results for a pK shift was also verified...
  20. ncbi Disentangling the web of allosteric communication in a homotetramer: heterotropic inhibition of phosphofructokinase from Bacillus stearothermophilus
    Allison D Ortigosa
    Department of Biochemistry and Biophysics and Center for Advanced Biomolecular Research, Texas A and M University, College Station, Texas 77843 2128, USA
    Biochemistry 43:577-86. 2004
    ..The results do not agree with either the concerted or sequential models that are often invoked to explain allosteric behavior in oligomeric enzymes...
  21. ncbi Low rate of glucose 6-phosphate hydrolysis in liver cells is a physiological feature of non-diabetic wild sand rats (Psammomys obesus)
    E A Koceir
    INSERM E 0221 Bioénergétique Fondamentale et Appliquée, Universite Joseph Fourier, Grenoble, France
    Diabetes Metab 29:363-74. 2003
    ..Psammomys obesus is a natural model of insulin resistance when fed a rodent laboratory chow...
  22. ncbi Emetine inhibits glycolysis in isolated, perfused rat hearts
    Shujia J Pan
    Department of Kinesiology, University of Texas at Austin, Austin, TX, USA
    Cardiovasc Toxicol 3:311-8. 2003
    ..Homogenate centrifugation studies indicate that emetine acts on bound rather than unbound phosphofructokinase. The inhibition may be uncompetitive in nature...
  23. ncbi Crystal structure of Pyrococcus furiosus phosphoglucose isomerase. Implications for substrate binding and catalysis
    John M Berrisford
    Department of Molecular Biology and Biotechnology, Krebs Institute for Biomolecular Research, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom
    J Biol Chem 278:33290-7. 2003
    ....
  24. pmc Inactivation of sll1556 in Synechocystis strain PCC 6803 impairs isoprenoid biosynthesis from pentose phosphate cycle substrates in vitro
    Kelly Poliquin
    Department of Cell Biology and Molecular Genetics, Microbiology Building, Campus Dr, University of Maryland, College Park, MD 20742, USA
    J Bacteriol 186:4685-93. 2004
    ..Our findings are consistent with an alternate metabolic shunt for biosynthesis of isoprenoids...
  25. ncbi Effect of 6-phosphogluconate on phosphoglucose isomerase in rat brain in vitro and in vivo
    M K Gaitonde
    Department of Biochemistry, St George s Hospital Medical School, London, England
    J Neurochem 52:1348-52. 1989
    ..No significant changes were observed in the values of Km, Vmax, and Ki for phosphoglucose isomerase in the brain of 6-aminonicotinamide-treated rats.(ABSTRACT TRUNCATED AT 250 WORDS)..
  26. ncbi Crystal structure of human phosphoglucose isomerase and analysis of the initial catalytic steps
    A T Cordeiro
    Laboratory of Protein Crystallography and Structural Biology, Physics Institute of São Carlos, University of Sao Paulo USP, Av Trabalhador Saocarlense 400, PO Box 369, 13566 590 Sao Carlos SP, Brazil
    Biochim Biophys Acta 1645:117-22. 2003
    ....
  27. ncbi Enzyme-to-enzyme channeling in the early steps of glycolysis in rat pancreatic islets
    Willy J Malaisse
    Laboratory of Experimental Hormonology, Brussels Free University, Brussels, Belgium
    Endocrine 24:105-9. 2004
    ....
  28. ncbi Leishmania donovani phosphofructokinase. Gene characterization, biochemical properties and structure-modeling studies
    Claudia Lopez
    Instituto de Biologia Experimental, Universidad Central de Venezuela, Caracas, Venezuela
    Eur J Biochem 269:3978-89. 2002
    ..Modelling studies and site-directed mutagenesis were employed to shed light on the structural basis for the AMP effector specificity and on ATP/PPi specificity among PFKs...
  29. ncbi Regulation of sucrose and starch synthesis in wheat (Triticum aestivum L.) leaves: role of fructose 2,6-bisphosphate
    S J Trevanion
    IACR Rothamsted, Harpenden, Hertfordshire, AL5 2JQ, UK
    Planta 215:653-65. 2002
    ..However F26BP does not appear to be involved in regulating the partitioning of fixed carbon between sucrose and starch in wheat under the experimental conditions examined...
  30. ncbi Exploring the active site of Trypanosoma brucei phosphofructokinase by inhibition studies: specific irreversible inhibition
    Samantha Claustre
    Laboratoire Synthèse et Physico Chimie de Molécules d Intérêt Biologique UMR CNRS 5068, Universite Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France
    Biochemistry 41:10183-93. 2002
    ..This first example of a specific irreversible inactivation of T. brucei PFK offers an opportunity to develop biologically active compounds against the sleeping sickness, the causative agent of which is the trypanosome...
  31. ncbi Integrated effects of multiple modulators on human liver glycogen phosphorylase a
    Nacide Ercan-Fang
    Metabolic Research Laboratory and Section of Endocrinology, Metabolism and Nutrition, Minneapolis Veterans Affairs Medical Center and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA
    Am J Physiol Endocrinol Metab 283:E29-37. 2002
    ..Of these effectors, only glucose changes greatly in vivo. Thus it may be the major regulator of HLGPa activity...
  32. ncbi Isomerase activity of the C-terminal fructose-6-phosphate binding domain of glucosamine-6-phosphate synthase from Escherichia coli
    R Todorova
    Institute of Biophysics, Bulgarian Academy of Sciences, Sofia
    J Enzyme Inhib 16:373-80. 2001
    ..The fructose-6P binding domain possibly includes a regulatory site, different from the catalytic center of the enzyme...
  33. ncbi Control of phosphofructokinase from rat skeletal muscle. Effects of fructose diphosphate, AMP, ATP, and citrate
    K Tornheim
    J Biol Chem 251:7322-8. 1976
    ..Citrate is a potent inhibitor at physiological concentrations, whereas AMP is a strong activator. Both AMP and citrate affect the maximum velocity and have little effect on affinity of the enzyme for fructose diphosphate...
  34. pmc Isoenzymes of phosphofructokinase in the rat. Demonstration of the three non-identical subunits by biochemical, immunochemical and kinetic studies
    S Vora
    Biochem J 229:333-41. 1985
    ....
  35. pmc Crystal structures of the effector-binding domain of repressor Central glycolytic gene Regulator from Bacillus subtilis reveal ligand-induced structural changes upon binding of several glycolytic intermediates
    Pavlina Rezacova
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA
    Mol Microbiol 69:895-910. 2008
    ..Our results suggest that CggR stability and function may be modulated by various effectors in a complex fashion...
  36. ncbi Pyruvate kinase from Lactobacillus bulgaricus: possible regulation by competition between strong and weak effectors
    G Le Bras
    Laboratoire d Enzymologie du Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Biochimie 75:797-802. 1993
    ....
  37. ncbi Antibiotic overproduction in Streptomyces coelicolor A3 2 mediated by phosphofructokinase deletion
    Irina Borodina
    Center for Microbial Biotechnology, BioCentrum DTU, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
    J Biol Chem 283:25186-99. 2008
    ....
  38. ncbi Isozyme analysis of human polymorphonuclear leukocyte phosphofructokinase from insulin resistant individuals
    P Durante
    Instituto de Investigaciones Clinicas, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela
    Biochem Biophys Res Commun 225:975-82. 1996
    ....
  39. ncbi The glycosomal ATP-dependent phosphofructokinase of Trypanosoma brucei must have evolved from an ancestral pyrophosphate-dependent enzyme
    P A Michels
    International Institute of Cellular and Molecular Pathology and Laboratory of Biochemistry, Catholic University of Louvain, Brussels, Belgium
    Eur J Biochem 250:698-704. 1997
    ..brucei PFK shows many structural differences, even at the active site, when compared with other ATP-dependent PFKs. These differences offer great potential for the structure-based design of trypanocidal drugs...
  40. doi Effects of oral administration of sodium citrate or acetate to pigs on blood parameters, postmortem glycolysis, muscle pH decline, and quality attributes of pork
    J W Stephens
    Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506, USA
    J Anim Sci 86:1669-77. 2008
    ..10). There was no significant inhibition of the PFK enzyme by orally administered CIT or ACE; however, the PFK glycolytic metabolite data analysis indicated that PFK was a main regulatory enzyme in postmortem muscle...
  41. doi Peripheral glucose metabolism is altered by epileptic seizures
    Fernanda K S Pereira
    Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Avenida Antonio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil
    Metab Brain Dis 23:105-14. 2008
    ..6-fold higher than that observed in resistant rats and that the audiogenic stimulus led to decreased concentration of this receptor in the muscle of WAR animals...
  42. ncbi Muscle metabolic responses during 16 hours of intermittent heavy exercise
    H J Green
    Department of Kinesiology, University of Waterloo, Waterloo, ON N2L 3G1, Canada
    Can J Physiol Pharmacol 85:634-45. 2007
    ..It is concluded that repetitive heavy exercise results in less of a disturbance in phosphorylation potential, possibly as a result of increased mitochondrial respiration during the rest-to-work non-steady-state transition...
  43. ncbi Characterisation of glutamine fructose-6-phosphate amidotransferase (EC 2.6.1.16) and N-acetylglucosamine metabolism in Bifidobacterium
    Sophie Foley
    School of Life Sciences, Napier University, Edinburgh, EH10 5DT, UK
    Arch Microbiol 189:157-67. 2008
    ..This is of interest considering the apparently reduced coding potential in bifidobacteria for enzymes associated with glutamine metabolism...
  44. pmc The ancestral activation promiscuity of ADP-glucose pyrophosphorylases from oxygenic photosynthetic organisms
    Misty L Kuhn
    Department of Chemistry and Biochemistry, Loyola University Chicago, 1032 W, Sheridan Rd, Chicago, IL 60660, USA
    BMC Evol Biol 13:51. 2013
    ....
  45. pmc Structure-based development of small molecule PFKFB3 inhibitors: a framework for potential cancer therapeutic agents targeting the Warburg effect
    Minsuh Seo
    Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, United States of America
    PLoS ONE 6:e24179. 2011
    ..This study validates PFKFB3 as a target for new cancer therapies and provides a framework for future development efforts...
  46. pmc Resolving the sources of plasma glucose excursions following a glucose tolerance test in the rat with deuterated water and [U-13C]glucose
    Teresa C Delgado
    Intermediary Metabolism Group, Center for Neurosciences and Cell Biology, Coimbra, Portugal
    PLoS ONE 7:e34042. 2012
    ..In rats that were placed on high-fat diet, the development of glucose intolerance was associated with a significantly higher gluconeogenic contribution to plasma glucose levels after the load...
  47. ncbi Changes in the contents of metabolites and enzyme activities in rice plants responding to Rhizoctonia solani Kuhn infection: activation of glycolysis and connection to phenylpropanoid pathway
    J Musembi Mutuku
    Saga University, Faculty of Agriculture, 1 Honjo Machi, Saga City, 840 8502 Japan
    Plant Cell Physiol 53:1017-32. 2012
    ....
  48. pmc The glycosylation pathway of Eimeria tenella is upregulated during gametocyte development and may play a role in oocyst wall formation
    Robert A Walker
    Institute for the Biotechnology of Infectious Diseases, University of Technology, Sydney, New South Wales 2007, Australia
    Eukaryot Cell 9:127-35. 2010
    ..tenella sexual stages and may play a role in directing a number of macrogamete proteins to the developing oocyst wall...
  49. doi Clotrimazole potentiates the inhibitory effects of ATP on the key glycolytic enzyme 6-phosphofructo-1-kinase
    Mariah Celestino Marcondes
    Laboratório de Oncobiologia Molecular LabOMol and Laboratório de Enzimologia e Controle do Metabolismo LabECoM, Departamento de Fármacos, Faculdade de Farmacia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941 590, Brazil
    Arch Biochem Biophys 497:62-7. 2010
    ..Altogether, the results presented here suggest that PFK inhibition by CTZ involves a decrease in the affinity of PFK for its substrates at the catalytic site with the concomitant potentiation of the inhibitory properties of ATP...
  50. ncbi Methylglyoxal is an intermediate in the biosynthesis of 6-deoxy-5-ketofructose-1-phosphate: a precursor for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii
    Robert H White
    Department of Biochemistry 0308, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA
    Biochemistry 45:12366-79. 2006
    ..This work is the first report of methylglyoxal functioning in central metabolism...
  51. pmc The crystal complex of phosphofructokinase-2 of Escherichia coli with fructose-6-phosphate: kinetic and structural analysis of the allosteric ATP inhibition
    Ricardo Cabrera
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
    J Biol Chem 286:5774-83. 2011
    ..Differences and resemblances in the patterns of fructose-6-P binding and the mechanism of inhibition are discussed for Pfk-1 and Pfk-2, as an example of evolutionary convergence, because these enzymes do not share a common ancestor...
  52. ncbi The crystal structure of mouse phosphoglucose isomerase at 1.6A resolution and its complex with glucose 6-phosphate reveals the catalytic mechanism of sugar ring opening
    J T Graham Solomons
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    J Mol Biol 342:847-60. 2004
    ..The structure with G6P also explains the specificity of PGI for glucose 6-phosphate over mannose 6-isomerase (M6P). To isomerize M6P to F6P requires a rotation of its C2-C3 bond but in PGI this is sterically blocked by Gln511...
  53. doi Characterization of hyperthermostable fructose-1,6-bisphosphatase from Thermococcus onnurineus NA1
    Yeol Gyun Lee
    Division of Life Science, Korea Basic Science Institute, Daejeon, 305 806, Republic of Korea
    J Microbiol 48:803-7. 2010
    ..However, this enzyme shows a different range of substrate specificities. These results suggest that TNA1-Fbp can establish new criterion for class V FBPases...
  54. pmc Glucose-6-phosphate isomerase deficiency results in mTOR activation, failed translocation of lipin 1α to the nucleus and hypersensitivity to glucose: Implications for the inherited glycolytic disease
    Jorge F Haller
    Department of Physiology and Biophysics, Boston University School of Medicine, USA
    Biochim Biophys Acta 1812:1393-402. 2011
    ..These results shed light on the mechanism underlying the pathologies associated with inherited GPI deficiency and the variability in the severity of the symptoms observed in these patients...
  55. ncbi Estimation of binding constants for the substrate and activator of Rhodobacter sphaeroides adenosine 5'-diphosphate-glucose pyrophosphorylase using affinity capillary electrophoresis
    John Kaddis
    Department of Chemistry and Biochemistry, California State University at Los Angeles, 5151 State University Drive, Los Angeles, CA 90032 8202, USA
    Anal Biochem 327:252-60. 2004
    ..This method demonstrates the quantitative ability of ACE to study different binding sites/ligand interactions in allosteric enzymes...
  56. ncbi Gene cloning and catalysis features of a new mannitol-1-phosphate dehydrogenase (BbMPD) from Beauveria bassiana
    Zheng Liang Wang
    Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, People s Republic of China
    Carbohydr Res 345:50-4. 2010
    ..0 and was much more sensitive to Cu(2+) and Zn(2+) than to Li(+) and Mn(2+). The results indicate a crucial role of BbMPD in the mannitol biosynthesis of B. bassiana...
  57. doi The crystal structure of ATP-bound phosphofructokinase from Trypanosoma brucei reveals conformational transitions different from those of other phosphofructokinases
    Iain W McNae
    Structural Biochemistry Group, Institute of Structural and Molecular Biology, University of Edinburgh, King s Buildings, Edinburgh, Scotland
    J Mol Biol 385:1519-33. 2009
    ..brucei PFK compared with bacterial and mammalian PFKs give optimism for the discovery of species-specific drugs for the treatment of diseases caused by protist parasites of the trypanosomatid family...
  58. doi Simultaneous quantification of metabolites involved in central carbon and energy metabolism using reversed-phase liquid chromatography-mass spectrometry and in vitro 13C labeling
    Wen Chu Yang
    Bindley Bioscience Center, Purdue University, West Lafayette, Indiana 47907, USA
    Anal Chem 80:9508-16. 2008
    ..Thirty common metabolites involved in glycolysis, the pentose phosphate pathway, and tricarboxylic acid cycle were identified and quantified from yeast lysate with a relative standard deviation of less than 10%...
  59. ncbi Noninvasive analysis of hepatic glycogen kinetics before and after breakfast with deuterated water and acetaminophen
    John G Jones
    NMR Research Unit, Department of Biochemistry and Center for Neurosciences and Cell Biology, Faculty of Sciences and Technology, University of Coimbra, 3001 401 Coimbra, Portugal
    Diabetes 55:2294-300. 2006
    ..Unlike current measurements of human hepatic glycogen metabolism, the (2)H(2)O/acetaminophen assay does not require specialized on-site clinical equipment or personnel...
  60. ncbi Evidence for impaired gluconeogenesis in very long-chain acyl-CoA dehydrogenase-deficient mice
    U Spiekerkoetter
    Department of General Pediatrics, University Children s Hospital, Moorenstrasse 5, 40225 Dusseldorf, Germany
    Horm Metab Res 38:625-30. 2006
    ..Whether this is due to lack of a substrate, inhibitory effects on other gluconeogenic enzymes or impaired transcription of gluconeogenic enzymes needs to be resolved in the future...
  61. doi Molecular cloning and characterization of Plasmodium falciparum transketolase
    Shweta Joshi
    Division of Biochemistry, Central Drug Research Institute, Lucknow 226001, Uttar Pradesh, India
    Mol Biochem Parasitol 160:32-41. 2008
    ..The availability of a structural model of PfTk and the observed differences in its kinetic properties compared to the host enzyme may facilitate designing of novel inhibitors of PfTk with potential anti-malarial activity...
  62. ncbi Crystallization and preliminary X-ray analysis of the two domains of glucosamine-6-phosphate synthase from Escherichia coli
    G Obmolova
    European Molecular Biology Laboratory, c o DESY, Hamburg, Germany
    J Mol Biol 242:703-5. 1994
    ..5 A, c = 334.3 A and with two molecules in the asymmetric unit. Diffraction data to 2.6 A resolution have been collected...
  63. ncbi Revised procedures for yeast metabolites extraction: application to a glucose pulse to carbon-limited yeast cultures, which reveals a transient activation of the purine salvage pathway
    Marie Odile Loret
    Laboratoire de Biotechnologie et Bioprocédés, UMR CNRS 5504, UMR INRA 792, Avenue de Rangueil, 31077 Toulouse Cedex 04, France
    Yeast 24:47-60. 2007
    ..This metabolic perturbation was accompanied by a rapid increase in the activity of the ISN1-encoded specific IMP-5'-nucleotidase. The mechanism of this activation remains to be determined...
  64. ncbi Human red blood cells targeted metabolome analysis of glycolysis cycle metabolites by capillary electrophoresis using an indirect photometric detection method
    Michał J Markuszewski
    Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdansk, Gen J Hallera 107, 80 416 Gdansk, Poland
    J Pharm Biomed Anal 39:636-42. 2005
    ..6 microM for fructose-6-phosphate (F-6-P) to 3.1 mM for 2,3-diphospho-D-glyceric acid (2,3-DPG). The method can be readily applied in clinical, pathophysiological and epidemiological studies...
  65. ncbi Endogenous effectors of human liver glycogen phosphorylase modulate effects of indole-site inhibitors
    Nacide Ercan-Fang
    Metabolic Research Laboratory, Section of Endocrinology, Metabolism, and Nutrition, Minneapolis Veterans Affairs Medical Center, Minneapolis, MN 55417, USA
    Am J Physiol Endocrinol Metab 289:E366-72. 2005
    ..However, at higher concentrations (10-30 microM), the GPI effect was dominant and resulted in inhibition of phosphorylase a activity irrespective of the presence or absence of the other modulators of the enzyme...
  66. ncbi Anomeric specificity of D-glucose production by rat hepatocytes
    W J Malaisse
    Laboratory of Experimental Hormonology ULB and High Resolution NMR Centre VUB, Brussels Free Universities, Brussels, Belgium
    Mol Cell Biochem 266:145-50. 2004
    ....
  67. ncbi Strain and near attack conformers in enzymic thiamin catalysis: X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor aldo
    Peter Asztalos
    Institut für Biochemie Biotechnologie, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06120 Halle Saale, Germany
    Biochemistry 46:12037-52. 2007
    ....
  68. ncbi Expression and purification of active human internal His(6)-tagged L-glutamine: D-Fructose-6P amidotransferase I
    Céline Richez
    Institut de Chimie des Substances Naturelles, ICSN CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Protein Expr Purif 54:45-53. 2007
    ..The stimulation of hydrolysis of the alternate substrate L-glutamine para-nitroanilide by D-fructose-6P (Fru-6P) afforded a K(d) of 5 microM for Fru-6P...
  69. ncbi Portal vein caffeine infusion enhances net hepatic glucose uptake during a glucose load in conscious dogs
    R Richard Pencek
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 0615, USA
    J Nutr 134:3042-6. 2004
    ..5 +/- 3.8 vs. 5.5 +/- 1.5 micromol/(kg . min)]. These findings indicate that physiologic circulating levels of caffeine can enhance NHGU during a glucose load, and the added glucose consumed by the liver is in part converted to lactate...
  70. ncbi Unfolding pathway of the dimeric and tetrameric forms of phosphofructokinase-2 from Escherichia coli
    Mauricio Baez
    Departamento de Biologia, Facultad de Ciencias Universidad de Chile, Casilla 653, Santiago, Chile
    Biochemistry 46:6141-8. 2007
    ....
  71. ncbi In silico exploration of the fructose-6-phosphate phosphorylation step in glycolysis: genomic evidence of the coexistence of an atypical ATP-dependent along with a PPi-dependent phosphofructokinase in Propionibacterium freudenreichii subsp. shermanii
    Guillaume Meurice
    INRA STLO, Rennes Cedex, France
    In Silico Biol 4:517-28. 2004
    ..2001]. The possible significance of the co-existence of these two PFKs and their importance for the regulation of glycolytic pathway flux in P. shermanii is discussed...
  72. doi Fructose 1,6-bisphosphate aldolase/phosphatase may be an ancestral gluconeogenic enzyme
    Rafael F Say
    Mikrobiologie, Fakultat Biologie, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    Nature 464:1077-81. 2010
    ..We propose that this highly conserved, heat-stabile and bifunctional FBP aldolase/phosphatase represents the pace-making ancestral gluconeogenic enzyme, and that in evolution gluconeogenesis preceded glycolysis...
  73. ncbi Effects of hyperglycemia on hepatic gluconeogenic flux during glycogen phosphorylase inhibition in the conscious dog
    Dale S Edgerton
    Department of Molecular Physiology and Biophysics, 702 Light Hall, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Am J Physiol Endocrinol Metab 286:E510-22. 2004
    ..We conclude that, in the absence of appreciable glycogen breakdown, the increase in glycolytic flux that accompanies hyperglycemia results in decreased net carbon flux to G-6-P but no effect on gluconeogenic flux to G-6-P...
  74. ncbi Structure of a ternary complex of an allosteric lactate dehydrogenase from Bacillus stearothermophilus at 2.5 A resolution
    D B Wigley
    Department of Biochemistry, University of Leicester, U K
    J Mol Biol 223:317-35. 1992
    ..His188 is connected to His195 (which binds the carbonyl group of the substrate) and Arg173 is connected to Arg171 (the residue that binds the carboxylate group of the substrate)...
  75. ncbi The allosteric transition of glucosamine-6-phosphate deaminase: the structure of the T state at 2.3 A resolution
    E Horjales
    Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico UNAM, Cuernavaca, Mor, Mexico
    Structure 7:527-37. 1999
    ..The reversibility of the catalysis and its rapid-equilibrium random kinetic mechanism, among other properties, make this enzyme a good model for studying allosteric processes...
  76. ncbi Chemical modification of SH groups of E. coli phosphofructokinase-2 induces subunit dissociation: monomers are inactive but preserve ligand binding properties
    V Guixe
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Arch Biochem Biophys 376:313-9. 2000
    ..These results show the presence of SH groups in the interface of Pfk-2 subunits, critical for subunit interactions, and that conformational changes occurring through the dimers are essential for catalytic activity...
  77. pmc Hepatic intralobular mapping of fructose metabolism in the rat liver
    S P Burns
    Cell Regulation Unit, Department of Diabetes and Metabolic Medicine, 5th Floor Alexandra Wing, St Bartholomew s and the Royal London School of Medicine and Dentistry, Whitechapel Road, London E1 1BB, UK
    Biochem J 349:539-45. 2000
    ..It may be inferred that fructokinase activity is expressed throughout the hepatic lobule...
  78. ncbi Sterol regulatory element-binding protein-1 is regulated by glucose at the transcriptional level
    A H Hasty
    Department of Metabolic Diseasese, University of Tokyo, Tokyo 113 8655, Japan
    J Biol Chem 275:31069-77. 2000
    ..These data provide strong evidence for the induction of SREBP-1c mRNA by glucose leading to increased mature protein in the nucleus, thus providing a potential mechanism for the up-regulation of lipogenic genes by glucose in vivo...
  79. ncbi Regulation of glycolysis during acclimation of scallops (Patinopecten yessoensis Jay) to anaerobiosis
    T Enomoto
    Department of Food Science, Ishikawa Agricultural College, Nonoichi, Japan
    Comp Biochem Physiol B Biochem Mol Biol 127:45-52. 2000
    ..7.1.11) (PFK1) was strongly activated by 1 microM Fru 2,6-BP when 0.2 mM Fru 6-P was used as a substrate, but the activity was not affected at 5 mM Fru 6-P. In view of these results, the regulation mechanism of glycolysis is discussed...
  80. ncbi Multisite control of the Crabtree effect in ascites hepatoma cells
    S Rodriguez-Enriquez
    Departamento de Bioquimica, Instituto Nacional de Cardiologia, Mexico
    Eur J Biochem 268:2512-9. 2001
    ..6 mM) at pH 6.8, exhibited a 40% inhibition of oxidative phosphorylation. The data suggest that the Crabtree effect is the result of several small metabolic changes promoted by addition of exogenous glucose or fructose...
  81. ncbi Reaction path of phosphofructo-1-kinase is altered by mutagenesis and alternative substrates
    X Wang
    Departments of Microbiology and Immunology and Biochemistry and Molecular Biology, The Chicago Medical School, North Chicago, Illinois 60064, USA
    Biochemistry 40:3938-42. 2001
    ..These unusual kinetic patterns could be interpreted within the random, steady-state model as reflecting changes in the rates of particular binding and catalytic events...
  82. ncbi Persistent binding of MgADP to the E187A mutant of Escherichia coli phosphofructokinase in the absence of allosteric effects
    A S Pham
    Division of Pathology and Laboratory Medicine, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Biochemistry 40:4140-9. 2001
    ..These results clarified the mechanism of a three-ligand interaction and offered a nontraditional perspective on allosteric mechanism...
  83. ncbi Photoaffinity labeling and photoaffinity cross-linking of phosphofructokinase-1 from Saccharomyces cerevisiae by 8-azidoadeninenucleotides
    M Knoche
    , Fachbereich Chemie und Pharmazie, , Germany
    Arch Biochem Biophys 385:301-10. 2001
    ....
  84. ncbi Triosephosphate metabolism by mature boar spermatozoa
    A R Jones
    Department of Biochemistry, University of Sydney, NSW, Australia
    Reprod Fertil Dev 9:577-81. 1997
    ..This suggests that these cells may have the capacity to convert glycolytic intermediates into a storage metabolite to conserve carbon atoms for the eventual synthesis of lactate...
  85. ncbi Inhibition of phosphomannose isomerase by fructose 1-phosphate: an explanation for defective N-glycosylation in hereditary fructose intolerance
    J Jaeken
    Department of Pediatrics, University of Leuven, Belgium
    Pediatr Res 40:764-6. 1996
    ..3.1.8), the first enzyme of the N-glycosylation pathway thus explaining the N-glycosylation disturbances in HFI...
  86. ncbi Yeast phosphofructokinase: kinetic characterization of a substrate-imprinted enzyme conformation
    T Kriegel
    Institut fur Biochemie, Universitat Leipzig, FRG
    Biomed Biochim Acta 50:1159-65. 1991
    ....
  87. pmc Crystal structure of the neutral form of fructose-1,6-bisphosphatase complexed with the product fructose 6-phosphate at 2.1-A resolution
    H M Ke
    Gibbs Chemical Laboratory, Harvard University, Cambridge, MA 02138
    Proc Natl Acad Sci U S A 88:2989-93. 1991
    ..Possible functions of the active-site residues are discussed, and candidates are suggested for site-directed mutagenesis...
  88. ncbi Glucosamine-6-phosphate synthase from Escherichia coli yields two proteins upon limited proteolysis: identification of the glutamine amidohydrolase and 2R ketose/aldose isomerase-bearing domains based on their biochemical properties
    M A Denisot
    Laboratoire de Bioorganique et Biotechnologies, UA CNRS 1389, Paris, France
    Arch Biochem Biophys 288:225-30. 1991
    ....
  89. ncbi Control of the fructose 6-phosphate/fructose 2,6-bisphosphate cycle by sn-glycerol 3-phosphate
    J Frenzel
    Institut fur Biochemie, Karl Marx Universität, Leipzig
    Biomed Biochim Acta 47:461-70. 1988
    ..In addition, sn-glycerol 3-phosphate was found to increase the concentration control coefficient of Fru 2,6-P2 with respect to Fru 6-P...
  90. ncbi Influence of ligands on the aggregation of the normal and mutant forms of phosphofructokinase 2 of Escherichia coli
    V Guixe
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago
    Arch Biochem Biophys 264:519-24. 1988
    ..Possibly Pfk-2* is not able to form a tetramer because of an alteration in the regulatory site for the Mg-nucleotide complex...
  91. pmc Localization of phosphoglucose isomerase in Escherichia coli and its relation to the induction of the hexose phosphate transport system
    I Friedberg
    J Bacteriol 112:1201-5. 1972
    ..S6P was not taken by intact cells. The data indicate that the isomerization of F6P to G6P can take place on the cell surface, and this explains the indirect induction of hexose phosphate transport by F6P...
  92. pmc Partial purification and regulatory properties of phosphofructokinase from Aspergillus niger
    A Habison
    Biochem J 209:669-76. 1983
    ....
  93. ncbi Changes of liver metabolite concentrations in adults with disorders of fructose metabolism after intravenous fructose by 31P magnetic resonance spectroscopy
    P Boesiger
    Institute of Biomedical Engineering and Medical Informatics, University of Zurich, Switzerland
    Pediatr Res 36:436-40. 1994
    ..abstract truncated at 250 words)..
  94. ncbi Effects of temperature shifts on the activities of Neurospora crassa glycogen synthase, glycogen phosphorylase and trehalose-6-phosphate synthase
    M A Noventa-Jordão
    Departamento de Biologia, Faculdade de Filosofia, Universidade de Sao Paulo, Ribeirao Preto, Brasil
    FEBS Lett 378:32-6. 1996
    ..Altogether, these results may contribute to explain the preferential accumulation of trehalose 45 degrees C, or that of glycogen at 30 degrees C...
  95. ncbi Crystal structure of rabbit phosphoglucose isomerase complexed with its substrate D-fructose 6-phosphate
    J H Lee
    Laboratory for Molecular Biology, MC567, Department of Biology, University of Illinois at Chicago, Chicago, Illinois 60607, USA
    Biochemistry 40:7799-805. 2001
    ....
  96. ncbi A comparative study on diurnal changes in metabolite levels in the leaves of three crassulacean acid metabolism (CAM) species, Ananas comosus, Kalanchoë daigremontiana and K. pinnata
    Li Song Chen
    Faculty of Agriculture, Saga University, Saga, 840 8502 Japan
    J Exp Bot 53:341-50. 2002
    ..Diurnal changes in oxaloacetate (OAA), pyruvate (Pyr) and phosphoenolpyruvate (PEP) levels in the three CAM species were similar...
  97. ncbi Differential effects of glucose and fructose on hexose metabolism in dog spermatozoa
    T Rigau
    Unit of Animal Reproduction, Department of Animal Medicine and Surgery, Autonomous University of Barcelona, E 08193 Bellaterra, Spain
    Reproduction 123:579-91. 2002
    ..Moreover, the differential hexose metabolism induced by the two sugars had distinct effects on the function of dog spermatozoa, as revealed by the diverse patterns of tyrosine phosphorylation...
  98. ncbi Bifunctional phosphomannose isomerase/GDP-D-mannose pyrophosphorylase is the point of control for GDP-D-mannose biosynthesis in Helicobacter pylori
    Bingyuan Wu
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    FEBS Lett 519:87-92. 2002
    ..The inhibition is of non-competitive (mixed) type. As the enzyme is found only in bacteria probably participating in capsular polysaccharide biosynthesis, it could be a specific therapeutic target against bacterial infection...
  99. ncbi Isolation of a single activating allosteric interaction in phosphofructokinase from Escherichia coli
    Aron W Fenton
    Department of Biochemistry and Biophysics and the Center for Advanced Biomolecular Research, Texas A and M University, 2128 TAMU, College Station, Texas 77843 2128, USA
    Biochemistry 41:13410-6. 2002
    ..56 kcal/mol under these conditions, indicating that the effects of MgADP are diminished by a homotropic activation equal to -1.3 kcal/mol. These data are not consistent with a concerted allosteric mechanism...
  100. ncbi A radioassay for phosphofructokinase-1 activity in cell extracts and purified enzyme
    Mauro Sola-Penna
    Laboratório de Enzimologia e Controle do Metabolismo LabECoM, Departamento de Fármacos, Faculdade de Farmacia, Universidade Federal do Rio de Janeiro, Ilha do Fundao, Rio de Janeiro, RJ 21944 910, Brazil
    J Biochem Biophys Methods 50:129-40. 2002
    ..The proposed method is precise and relatively inexpensive, and can be applied to determine phosphofructokinase-1 activity in cellular extracts as well as in the purified enzyme...
  101. ncbi Biosynthesis of 1-deoxy-1-imino-D-erythrose 4-phosphate: a defining metabolite in the aminoshikimate pathway
    Jiantao Guo
    Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA
    J Am Chem Soc 124:528-9. 2002
    ..mediterranei crude cell lysate. Retention of both 15N and 2H2 labeling in product aminoDAHP indicates that 3-amino-3-deoxy-d-fructose 6-phosphate is serving as a sequestered form of 1-deoxy-1-imino-d-erythrose 4-phosphate...

Research Grants1

  1. STRUCTURE OF ORAL BACTERIAL ADHESINS
    Teresa Ruiz; Fiscal Year: 2008
    ..We will determine the 3D structure of the adhesins and adhesin/receptor complexes, which is essential to gain insights into the different stages of the pathogenic process. ..