fructosephosphates

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Top Publications

  1. ncbi The crystal and solution studies of glucosamine-6-phosphate synthase from Candida albicans
    Joanna Raczynska
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, ul Noskowskiego 12 14, 61 704 Poznan, Poland
    J Mol Biol 372:672-88. 2007
  2. ncbi Crystal structure of glucokinase regulatory protein
    Alexander Pautsch
    Departments of Lead Identification and Optimization Support, CardioMetabolic Diseases Research, CNS Diseases Research, Drug Discovery Support, BP Process Science, and Medicinal Chemistry, Boehringer Ingelheim Pharma GmbH and Company KG, Biberach an der Riss, Germany
    Biochemistry 52:3523-31. 2013
  3. pmc Differences in regulatory properties between human and rat glucokinase regulatory protein
    Katy J Brocklehurst
    Cardiovascular and Gastrointestinal Department, AstraZeneca, Macclesfield, Cheshire SK10 4TG, UK
    Biochem J 378:693-7. 2004
  4. ncbi Structure and catalytic mechanism of glucosamine 6-phosphate deaminase from Escherichia coli at 2.1 A resolution
    G Oliva
    Instituto de Fisica de Sao Carlos, Universidade de Sao Paulo, Brazil
    Structure 3:1323-32. 1995
  5. ncbi Identification of fructose 6-phosphate- and fructose 1-phosphate-binding residues in the regulatory protein of glucokinase
    Maria Veiga-da-Cunha
    Laboratory of Physiological Chemistry, University of Louvain and Christian de Duve Institute of Cellular Pathology, B 1200 Brussels, Belgium
    J Biol Chem 277:8466-73. 2002
  6. ncbi Accumulation of glucose 6-phosphate or fructose 6-phosphate is responsible for destabilization of glucose transporter mRNA in Escherichia coli
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    J Biol Chem 278:15608-14. 2003
  7. ncbi Crystal structure of the hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3): a possible new target for cancer therapy
    Song Gun Kim
    Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA
    J Biol Chem 281:2939-44. 2006
  8. ncbi Involvement of the C terminus in intramolecular nitrogen channeling in glucosamine 6-phosphate synthase: evidence from a 1.6 A crystal structure of the isomerase domain
    A Teplyakov
    European Molecular Biology Laboratory, Germany
    Structure 6:1047-55. 1998
  9. ncbi A direct substrate-substrate interaction found in the kinase domain of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
    Song Gun Kim
    Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    J Mol Biol 370:14-26. 2007
  10. ncbi Isozyme analysis of human polymorphonuclear leukocyte phosphofructokinase from insulin resistant individuals
    P Durante
    Instituto de Investigaciones Clinicas, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela
    Biochem Biophys Res Commun 225:975-82. 1996

Scientific Experts

Detail Information

Publications164 found, 100 shown here

  1. ncbi The crystal and solution studies of glucosamine-6-phosphate synthase from Candida albicans
    Joanna Raczynska
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, ul Noskowskiego 12 14, 61 704 Poznan, Poland
    J Mol Biol 372:672-88. 2007
    ..This study of Gfa1p focuses on the features that distinguish it from the prokaryotic homologue in terms of quaternary structure, control of the enzymatic activity and details of the isomerase active site...
  2. ncbi Crystal structure of glucokinase regulatory protein
    Alexander Pautsch
    Departments of Lead Identification and Optimization Support, CardioMetabolic Diseases Research, CNS Diseases Research, Drug Discovery Support, BP Process Science, and Medicinal Chemistry, Boehringer Ingelheim Pharma GmbH and Company KG, Biberach an der Riss, Germany
    Biochemistry 52:3523-31. 2013
    ..The structure of the inactive GKRP-F1P complex provides a starting point for understanding the mechanism of fructose phosphate-dependent GK regulation at an atomic level. ..
  3. pmc Differences in regulatory properties between human and rat glucokinase regulatory protein
    Katy J Brocklehurst
    Cardiovascular and Gastrointestinal Department, AstraZeneca, Macclesfield, Cheshire SK10 4TG, UK
    Biochem J 378:693-7. 2004
    ..Residues that are not conserved between the rodent and human proteins affect both the affinity for fructose 6-phosphate and sorbitol 6-phosphate and the inhibitory potency of GKRP on glucokinase in the absence of these ligands...
  4. ncbi Structure and catalytic mechanism of glucosamine 6-phosphate deaminase from Escherichia coli at 2.1 A resolution
    G Oliva
    Instituto de Fisica de Sao Carlos, Universidade de Sao Paulo, Brazil
    Structure 3:1323-32. 1995
    ..The determination of the structure of this protein provides fundamental knowledge for understanding its mode of action and the nature of allosteric conformational changes that regulate its function...
  5. ncbi Identification of fructose 6-phosphate- and fructose 1-phosphate-binding residues in the regulatory protein of glucokinase
    Maria Veiga-da-Cunha
    Laboratory of Physiological Chemistry, University of Louvain and Christian de Duve Institute of Cellular Pathology, B 1200 Brussels, Belgium
    J Biol Chem 277:8466-73. 2002
    ..These results are consistent with GKRP having one single binding site for phosphate esters. They also show that a missense mutation of GKRP can lead to a gain of function...
  6. ncbi Accumulation of glucose 6-phosphate or fructose 6-phosphate is responsible for destabilization of glucose transporter mRNA in Escherichia coli
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    J Biol Chem 278:15608-14. 2003
    ..We conclude that the RNase E-dependent destabilization of ptsG mRNA occurs in response to accumulation of glucose-6-P or fructose-6-P...
  7. ncbi Crystal structure of the hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3): a possible new target for cancer therapy
    Song Gun Kim
    Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA
    J Biol Chem 281:2939-44. 2006
    ..This finding suggests that a molecule in which EDTA is covalently linked to ADP is a good starting molecule for the development of new cancer-therapeutic molecules...
  8. ncbi Involvement of the C terminus in intramolecular nitrogen channeling in glucosamine 6-phosphate synthase: evidence from a 1.6 A crystal structure of the isomerase domain
    A Teplyakov
    European Molecular Biology Laboratory, Germany
    Structure 6:1047-55. 1998
    ..Knowledge of the three-dimensional structure of GlmS is essential for understanding the general principles of catalysis by ketol isomerases and the mechanism of nitrogen transfer in glutamine amidotransferases...
  9. ncbi A direct substrate-substrate interaction found in the kinase domain of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
    Song Gun Kim
    Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    J Mol Biol 370:14-26. 2007
    ..The site-directed mutant study and inhibition kinetics suggest that this reaction will be catalyzed most efficiently by the protein when the substrates bind to the active pocket in an ordered manner in which ATP binds first...
  10. ncbi Isozyme analysis of human polymorphonuclear leukocyte phosphofructokinase from insulin resistant individuals
    P Durante
    Instituto de Investigaciones Clinicas, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela
    Biochem Biophys Res Commun 225:975-82. 1996
    ....
  11. ncbi Leishmania donovani phosphofructokinase. Gene characterization, biochemical properties and structure-modeling studies
    Claudia Lopez
    Instituto de Biologia Experimental, Universidad Central de Venezuela, Caracas, Venezuela
    Eur J Biochem 269:3978-89. 2002
    ..Modelling studies and site-directed mutagenesis were employed to shed light on the structural basis for the AMP effector specificity and on ATP/PPi specificity among PFKs...
  12. ncbi Integrated effects of multiple modulators on human liver glycogen phosphorylase a
    Nacide Ercan-Fang
    Metabolic Research Laboratory and Section of Endocrinology, Metabolism and Nutrition, Minneapolis Veterans Affairs Medical Center and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA
    Am J Physiol Endocrinol Metab 283:E29-37. 2002
    ..Of these effectors, only glucose changes greatly in vivo. Thus it may be the major regulator of HLGPa activity...
  13. ncbi Isomerase activity of the C-terminal fructose-6-phosphate binding domain of glucosamine-6-phosphate synthase from Escherichia coli
    R Todorova
    Institute of Biophysics, Bulgarian Academy of Sciences, Sofia
    J Enzyme Inhib 16:373-80. 2001
    ..The fructose-6P binding domain possibly includes a regulatory site, different from the catalytic center of the enzyme...
  14. ncbi Expression of key substrate cycle enzymes in rat spermatogenic cells: fructose 1,6 bisphosphatase and 6 phosphofructose 1-kinase
    Alejandro J Yañez
    Instituto de Bioquimica, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile
    J Cell Physiol 212:807-16. 2007
    ....
  15. ncbi Pyruvate kinase from Lactobacillus bulgaricus: possible regulation by competition between strong and weak effectors
    G Le Bras
    Laboratoire d Enzymologie du Centre National de la Recherche Scientifique, Gif sur Yvette, France
    Biochimie 75:797-802. 1993
    ....
  16. ncbi Effects of oral administration of sodium citrate or acetate to pigs on blood parameters, postmortem glycolysis, muscle pH decline, and quality attributes of pork
    J W Stephens
    Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506, USA
    J Anim Sci 86:1669-77. 2008
    ..10). There was no significant inhibition of the PFK enzyme by orally administered CIT or ACE; however, the PFK glycolytic metabolite data analysis indicated that PFK was a main regulatory enzyme in postmortem muscle...
  17. ncbi The glycosomal ATP-dependent phosphofructokinase of Trypanosoma brucei must have evolved from an ancestral pyrophosphate-dependent enzyme
    P A Michels
    International Institute of Cellular and Molecular Pathology and Laboratory of Biochemistry, Catholic University of Louvain, Brussels, Belgium
    Eur J Biochem 250:698-704. 1997
    ..brucei PFK shows many structural differences, even at the active site, when compared with other ATP-dependent PFKs. These differences offer great potential for the structure-based design of trypanocidal drugs...
  18. pmc Crystal structures of the effector-binding domain of repressor Central glycolytic gene Regulator from Bacillus subtilis reveal ligand-induced structural changes upon binding of several glycolytic intermediates
    Pavlina Rezacova
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA
    Mol Microbiol 69:895-910. 2008
    ..Our results suggest that CggR stability and function may be modulated by various effectors in a complex fashion...
  19. ncbi MgATP-dependent activation by phosphoenolpyruvate of the E187A mutant of Escherichia coli phosphofructokinase
    A S Pham
    Division of Pathology and Laboratory Medicine, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Biochemistry 40:4150-8. 2001
    ..The present study examines the role of Glu 187 at the allosteric site in the binding of Fru-6-P and offers a more complex explanation of the mechanism than that described by traditional allosteric mechanistic models...
  20. ncbi A deeper investigation on carbohydrate cycling in Sinorhizobium meliloti
    I Gosselin
    , UMR CNRS 6022, , , 33 rue Saint Leu, 80039 Cedex, Amiens, France
    FEBS Lett 499:45-9. 2001
    ..Furthermore, carbohydrate cycling in S. meliloti is also observed on fructose, making the situation in this bacterium significantly different from that depicted for alginate-synthesizing species...
  21. ncbi Antibiotic overproduction in Streptomyces coelicolor A3 2 mediated by phosphofructokinase deletion
    Irina Borodina
    Center for Microbial Biotechnology, BioCentrum DTU, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
    J Biol Chem 283:25186-99. 2008
    ....
  22. ncbi Peripheral glucose metabolism is altered by epileptic seizures
    Fernanda K S Pereira
    Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Avenida Antonio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil
    Metab Brain Dis 23:105-14. 2008
    ..6-fold higher than that observed in resistant rats and that the audiogenic stimulus led to decreased concentration of this receptor in the muscle of WAR animals...
  23. ncbi Creation of an allosteric phosphofructokinase starting with a nonallosteric enzyme. The case of dictyostelium discoideum phosphofructokinase
    Belen Santamaria
    Departamento de Bioquímica and Instituto de Investigaciones Biomédicas Alberto Sols UAM CSIC, Facultad de Medicina de la Universidad Autonoma, 28029 Madrid, Spain
    J Biol Chem 277:1210-6. 2002
    ....
  24. ncbi Characterisation of glutamine fructose-6-phosphate amidotransferase (EC 2.6.1.16) and N-acetylglucosamine metabolism in Bifidobacterium
    Sophie Foley
    School of Life Sciences, Napier University, Edinburgh, EH10 5DT, UK
    Arch Microbiol 189:157-67. 2008
    ..This is of interest considering the apparently reduced coding potential in bifidobacteria for enzymes associated with glutamine metabolism...
  25. ncbi Muscle metabolic responses during 16 hours of intermittent heavy exercise
    H J Green
    Department of Kinesiology, University of Waterloo, Waterloo, ON N2L 3G1, Canada
    Can J Physiol Pharmacol 85:634-45. 2007
    ..It is concluded that repetitive heavy exercise results in less of a disturbance in phosphorylation potential, possibly as a result of increased mitochondrial respiration during the rest-to-work non-steady-state transition...
  26. ncbi Purification and kinetic properties of 6-phosphofructo-1-kinase from gilthead sea bream muscle
    Dominica Mediavilla
    Departament de Bioquimica i Biologia Molecular, Facultat de Farmacia, Universitat de Barcelona, Diagonal 643, 08028 Barcelona, Spain
    Biochim Biophys Acta 1770:706-15. 2007
    ..Unlike the mammalian muscle enzyme, our findings support that phosphorylation of PFKM may exert a major role during starvation in fish muscle...
  27. ncbi Crystal structure of human phosphoglucose isomerase and analysis of the initial catalytic steps
    A T Cordeiro
    Laboratory of Protein Crystallography and Structural Biology, Physics Institute of São Carlos, University of Sao Paulo USP, Av Trabalhador Saocarlense 400, PO Box 369, 13566 590 Sao Carlos SP, Brazil
    Biochim Biophys Acta 1645:117-22. 2003
    ....
  28. ncbi The structures of inhibitor complexes of Pyrococcus furiosus phosphoglucose isomerase provide insights into substrate binding and catalysis
    John M Berrisford
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    J Mol Biol 343:649-57. 2004
    ..These similarities suggest that aspects of the catalytic mechanisms of these two structurally unrelated PGIs may be similar and based on an enediol intermediate...
  29. ncbi Enzyme-to-enzyme channeling in the early steps of glycolysis in rat pancreatic islets
    Willy J Malaisse
    Laboratory of Experimental Hormonology, Brussels Free University, Brussels, Belgium
    Endocrine 24:105-9. 2004
    ....
  30. pmc Inactivation of sll1556 in Synechocystis strain PCC 6803 impairs isoprenoid biosynthesis from pentose phosphate cycle substrates in vitro
    Kelly Poliquin
    Department of Cell Biology and Molecular Genetics, Microbiology Building, Campus Dr, University of Maryland, College Park, MD 20742, USA
    J Bacteriol 186:4685-93. 2004
    ..Our findings are consistent with an alternate metabolic shunt for biosynthesis of isoprenoids...
  31. ncbi Quantitative estimation of channeling from early glycolytic intermediates to CO in intact Escherichia coli
    Georgia Shearer
    Department of Biology, Washington University, St Louis, MO 63130, USA
    FEBS J 272:3260-9. 2005
    ..The data also suggest that, in addition to pathway enzymes being associated with one another, some are free in the aqueous cytosol. How sensitive the degree of channeling is to growth or experimental conditions remains to be determined...
  32. ncbi Emetine inhibits glycolysis in isolated, perfused rat hearts
    Shujia J Pan
    Department of Kinesiology, University of Texas at Austin, Austin, TX, USA
    Cardiovasc Toxicol 3:311-8. 2003
    ..Homogenate centrifugation studies indicate that emetine acts on bound rather than unbound phosphofructokinase. The inhibition may be uncompetitive in nature...
  33. ncbi Disentangling the web of allosteric communication in a homotetramer: heterotropic inhibition of phosphofructokinase from Bacillus stearothermophilus
    Allison D Ortigosa
    Department of Biochemistry and Biophysics and Center for Advanced Biomolecular Research, Texas A and M University, College Station, Texas 77843 2128, USA
    Biochemistry 43:577-86. 2004
    ..The results do not agree with either the concerted or sequential models that are often invoked to explain allosteric behavior in oligomeric enzymes...
  34. ncbi Excess mannose limits the growth of phosphomannose isomerase PMI40 deletion strain of Saccharomyces cerevisiae
    Juha Pekka Pitkänen
    MediCel Ltd, Haartmaninkatu 8, FIN 00290 Helsinki, Finland
    J Biol Chem 279:55737-43. 2004
    ....
  35. ncbi Effect of 6-phosphogluconate on phosphoglucose isomerase in rat brain in vitro and in vivo
    M K Gaitonde
    Department of Biochemistry, St George s Hospital Medical School, London, England
    J Neurochem 52:1348-52. 1989
    ..No significant changes were observed in the values of Km, Vmax, and Ki for phosphoglucose isomerase in the brain of 6-aminonicotinamide-treated rats.(ABSTRACT TRUNCATED AT 250 WORDS)..
  36. ncbi Development of a quantification system of ionic dissociative metabolites using an FT-IR/ATR method
    T Pan
    Department of Sustainable Resource Sciences, Faculty of Bioresources, Mie University, 1515 Kamihama cho, Tsu, Mie 514 8507, Japan
    Bioprocess Biosyst Eng 26:133-9. 2003
    ..In addition, the stability of quantification results for a pK shift was also verified...
  37. pmc Isoenzymes of phosphofructokinase in the rat. Demonstration of the three non-identical subunits by biochemical, immunochemical and kinetic studies
    S Vora
    Biochem J 229:333-41. 1985
    ....
  38. ncbi Crystal structure of Pyrococcus furiosus phosphoglucose isomerase. Implications for substrate binding and catalysis
    John M Berrisford
    Department of Molecular Biology and Biotechnology, Krebs Institute for Biomolecular Research, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom
    J Biol Chem 278:33290-7. 2003
    ....
  39. ncbi Control of phosphofructokinase from rat skeletal muscle. Effects of fructose diphosphate, AMP, ATP, and citrate
    K Tornheim
    J Biol Chem 251:7322-8. 1976
    ..Citrate is a potent inhibitor at physiological concentrations, whereas AMP is a strong activator. Both AMP and citrate affect the maximum velocity and have little effect on affinity of the enzyme for fructose diphosphate...
  40. ncbi Exploring the active site of Trypanosoma brucei phosphofructokinase by inhibition studies: specific irreversible inhibition
    Samantha Claustre
    Laboratoire Synthèse et Physico Chimie de Molécules d Intérêt Biologique UMR CNRS 5068, Universite Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France
    Biochemistry 41:10183-93. 2002
    ..This first example of a specific irreversible inactivation of T. brucei PFK offers an opportunity to develop biologically active compounds against the sleeping sickness, the causative agent of which is the trypanosome...
  41. ncbi [Recent advance in the discovery of allosteric inhibitors binding to the AMP site of fructose-1,6-bisphosphatase]
    Zhan Mei Li
    Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
    Yao Xue Xue Bao 46:1291-300. 2011
    ..Recent research efforts were reviewed in the field of developing allosteric inhibitors interacting with the AMP binding site of FBPase...
  42. pmc The utilization of fructose by Escherichia coli. Properties of a mutant defective in fructose 1-phosphate kinase activity
    T Ferenci
    Biochem J 132:341-7. 1973
    ..In contrast, the equally rapid but more extensive uptake of [(14)C]fructose by the parent organism required prior growth in the presence of fructose...
  43. ncbi Regulation of sucrose and starch synthesis in wheat (Triticum aestivum L.) leaves: role of fructose 2,6-bisphosphate
    S J Trevanion
    IACR Rothamsted, Harpenden, Hertfordshire, AL5 2JQ, UK
    Planta 215:653-65. 2002
    ..However F26BP does not appear to be involved in regulating the partitioning of fixed carbon between sucrose and starch in wheat under the experimental conditions examined...
  44. ncbi Salt-regulated mannitol metabolism in algae
    Koji Iwamoto
    Functional Biosciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
    Mar Biotechnol (NY) 7:407-15. 2005
    ..In this review we focus on the metabolism of mannitol, mainly in marine photosynthetic organisms, and suggest how this might be applied to producing salt-tolerant transgenic plants...
  45. ncbi Cooperative effect of fructose bisphosphate and glyceraldehyde-3-phosphate dehydrogenase on aldolase action
    J Neuzil
    Institute of Enzymology, Hungarian Academy of Sciences, Budapest
    Biochim Biophys Acta 1037:307-12. 1990
    ....
  46. pmc Examination of intrinsic sulfonamide resistance in Bacillus anthracis: a novel assay for dihydropteroate synthase
    Michelle Wright Valderas
    Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 Mc Elroy Hall, Stillwater, Oklahoma 74078, USA
    Biochim Biophys Acta 1780:848-53. 2008
    ..anthracis DHPS results in part from the use of the sulfonamides as alternative substrates, resulting in the formation of sulfonamide-pterin adducts, and not necessarily due to an inability to bind them...
  47. ncbi Profiling of pentose phosphate pathway intermediates in blood spots by tandem mass spectrometry: application to transaldolase deficiency
    Jojanneke H J Huck
    Department of Pediatrics, VU University Medical Centre, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands
    Clin Chem 49:1375-80. 2003
    ..We hypothesized that they are derived from sugar phosphate intermediates of the pentose phosphate pathway (PPP), and we developed a semiquantitative method for profiling of pentose phosphate pathway intermediates...
  48. ncbi Enzymic activities in two populations of purified rat islet beta-cells
    A Sener
    Laboratory of Experimental Medicine, Brussels Free University, B-1070 Brussels, Belgium
    Int J Mol Med 8:285-9. 2001
    ..These findings thus argue against a low expression of either low-Km hexokinase(s) or lactate dehydrogenase as major determinants of the glucose-sensing device in beta-cells...
  49. ncbi Carnosine protects against the inactivation of esterase induced by glycation and a steroid
    Hong Yan
    Nuffield Laboratory of Ophthalmology, University of Oxford, Walton St, Oxford OX2 6AW, UK
    Biochim Biophys Acta 1741:120-6. 2005
    ..Carnosine decreased the extent of the cross-linking. These results provide further evidence for carnosine's role as an anti-glycation compound. It is also proposed that carnosine may be an anti-steroid agent...
  50. ncbi Leishmania mexicana mexicana glucose-6-phosphate isomerase: crystallization, molecular-replacement solution and inhibition
    Artur T Cordeiro
    Laboratory of Protein Crystallography and Structural Biology, Physics Institute of São Carlos, University of Sao Paulo, USP, Av Trabalhador Saocarlense 400, PO Box 369, 13566 590 Sao Carlos SP, Brazil
    Acta Crystallogr D Biol Crystallogr 60:915-9. 2004
    ..3 A in space group P6(1), with unit-cell parameters a = b = 87.0, c = 354.7 A, alpha = beta = 90, gamma = 120 degrees. A preliminary study of the first inhibitors to be evaluated on the Leishmania enzyme is also reported...
  51. ncbi Low rate of glucose 6-phosphate hydrolysis in liver cells is a physiological feature of non-diabetic wild sand rats (Psammomys obesus)
    E A Koceir
    INSERM E 0221 Bioénergétique Fondamentale et Appliquée, Universite Joseph Fourier, Grenoble, France
    Diabetes Metab 29:363-74. 2003
    ..Psammomys obesus is a natural model of insulin resistance when fed a rodent laboratory chow...
  52. ncbi Inhibition of type I and type II phosphomannose isomerases by the reaction intermediate analogue 5-phospho-D-arabinonohydroxamic acid supports a catalytic role for the metal cofactor
    Celine Roux
    Laboratoire de Chimie Bioorganique et Bioinorganique, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8124, Institut de Chimie Moleculaire et des Materiaux d Orsay, Batiment 420, Universite Paris Sud XI, 91405 Orsay, France
    Biochemistry 43:2926-34. 2004
    ..All together, our results are consistent with a catalytic role for the metal cofactor in PMI activity...
  53. ncbi D-Fructose-6-phosphate aldolase-catalyzed one-pot synthesis of iminocyclitols
    Masakazu Sugiyama
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    J Am Chem Soc 129:14811-7. 2007
    ..Facile access to these compounds makes them attractive core structures for further inhibitor optimization...
  54. ncbi Characterization of the Aspergillus fumigatus phosphomannose isomerase Pmi1 and its impact on cell wall synthesis and morphogenesis
    Wenxia Fang
    Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China
    Microbiology 155:3281-93. 2009
    ..Our results reveal that PMI activity is essential for viability and plays a central regulatory role in both cell wall synthesis and energy production in A. fumigatus...
  55. ncbi The enzyme thermistor--a realistic biosensor concept. A critical review
    Maria Yakovleva
    Department of Biochemistry and Structural Biology, Lund University, PO Box 124, 22100 Lund, Sweden
    Anal Chim Acta 766:1-12. 2013
    ..Another recent method is the determination of fructose using a fructose-6-phosphate kinase column. Operation with complex sample matrices such as blood, plasma and milk and how to avoid non-specific temperature effects are considered...
  56. pmc Characterization of phosphofructokinase activity in Mycobacterium tuberculosis reveals that a functional glycolytic carbon flow is necessary to limit the accumulation of toxic metabolic intermediates under hypoxia
    Wai Yee Phong
    Novartis Institute for Tropical Diseases, Singapore, Singapore
    PLoS ONE 8:e56037. 2013
    ..Such toxic effect is exacerbated when the glycolytic pathway is disrupted at the PKF step...
  57. ncbi Affinity labeling of Escherichia coli glucosamine-6-phosphate synthase with a fructose 6-phosphate analog--evidence for proximity between the N-terminal cysteine and the fructose-6-phosphate-binding site
    C Leriche
    Institut de Chimie des Substances Naturelles, UPR 2301 CNRS, Gif sur Yvette, France
    Eur J Biochem 245:418-22. 1997
    ..These results provide evidence for the close proximity of glutamine and fructose-6-phosphate-binding sites recently suggested by Bearne [Bearne, S. L. (1996) J. Biol. Chem. 271, 3052-3057]...
  58. pmc Biosynthesis of levan, a bacterial extracellular polysaccharide, in the yeast Saccharomyces cerevisiae
    Jaco Franken
    Institute for Wine Biotechnology, Stellenbosch University, Stellenbosch, Western Cape, South Africa
    PLoS ONE 8:e77499. 2013
    ..Furthermore, the work provides interesting perspectives when considering the heterologous expression of sugar polymerizing enzymes in yeast. ..
  59. ncbi Muscle metabolism and performance improvement after two training programmes of sprint running differing in rest interval duration
    Ploutarchos Saraslanidis
    Department of Physical Education and Sport Science, Aristotle University of Thessaloniki, Thessaloniki, Greece
    J Sports Sci 29:1167-74. 2011
    ..This may be due to a greater activation of glycolysis caused, in part, by the limited resynthesis of phosphocreatine during the very short rest interval...
  60. ncbi In vitro binding of the pleiotropic transcriptional regulatory protein, FruR, to the fru, pps, ace, pts and icd operons of Escherichia coli and Salmonella typhimurium
    T M Ramseier
    Department of Biology, University of California, San Diego, La Jolla 92093 0116
    J Mol Biol 234:28-44. 1993
    ..Computer searches revealed the presence of this sequence in numerous functionally diverse operons, implying that FruR is a global transcriptional regulatory protein in enteric bacteria...
  61. ncbi Influence of MgADP on phosphofructokinase from Escherichia coli. Elucidation of coupling interactions with both substrates
    J L Johnson
    Department of Chemistry and Biochemistry, University of Oklahoma, Norman 73019
    Biochemistry 33:2635-43. 1994
    ..For both activating and inhibitory couplings, the enthalpy and entropy terms have the same sign as the coupling free energy.(ABSTRACT TRUNCATED AT 250 WORDS)..
  62. ncbi Properties of recombinant ATP-dependent fructokinase from the halotolerant methanotroph Methylomicrobium alcaliphilum 20Z
    S Y But
    Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr Nauki 5, 142290 Pushchino, Moscow Region, Russia
    Biochemistry (Mosc) 77:372-7. 2012
    ..The involvement of fructokinase in sucrose metabolism in M. alcaliphilum 20Z and other methanotrophs and autotrophs is discussed...
  63. ncbi Modulation of the interaction between aldolase and glycerol-phosphate dehydrogenase by fructose phosphates
    B G Vertessy
    Institute of Enzymology, Hungarian Academy of Sciences, Budapest
    Biochim Biophys Acta 1078:236-42. 1991
    ..The possible molecular mechanism of the fructose-1,6-bisphosphate modulated interaction is discussed...
  64. ncbi Active-site mutants altering the cooperativity of E. coli phosphofructokinase
    S A Berger
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 343:575-6. 1990
    ..These results show that each of the three arginines contributes to cooperativity and to the transmission of allosteric signals between the four subunit of the enzyme...
  65. ncbi Active-site remodelling in the bifunctional fructose-1,6-bisphosphate aldolase/phosphatase
    Juan Du
    Lehrstuhl für Biochemie, Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat Freiburg, Albertstrasse 21, 79104 Freiburg, Germany
    Nature 478:534-7. 2011
    ....
  66. ncbi Does regulatory protein play a role in glucokinase localization?
    F B Reitz
    Center for Bioengineering, University of Washington, Seattle 98195 7962, U S A
    Horm Metab Res 29:317-21. 1997
    ..These results suggest that glucokinase regulatory protein may play a role in metabolism-sensitive glucokinase localization in vivo...
  67. ncbi Crystal structure of a trapped phosphoenzyme during a catalytic reaction
    Y H Lee
    Nat Struct Biol 4:615-8. 1997
    ..The geometry suggests that the dephosphorylation step requires prior removal of the product for an 'associative in-line' phosphoryl transfer to the catalytic water...
  68. ncbi Functional nonequivalence of transketolase active centers
    German A Kochetov
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow, Russia
    IUBMB Life 62:797-802. 2010
    ..In this article, we review experimental evidence of functional nonequivalence of the two active centers of TK, which are known to be identical by crystal X-ray structure analysis...
  69. ncbi Identification and characterization of genes underlying chitinolysis in Collimonas fungivorans Ter331
    Kathrin Fritsche
    Netherlands Institute of Ecology NIOO KNAW, Heteren, The Netherlands
    FEMS Microbiol Ecol 66:123-35. 2008
    ..No link could be established between chitinolysis and antifungal activity of C. fungivorans Ter331, suggesting that the bacterium's reported antagonism towards fungi relies on other mechanisms...
  70. pmc Malate dehydrogenase of the cytosol. Preparation and reduced nicotinamide-adenine dinucleotide-binding studies
    A Lodola
    Biochem J 169:577-88. 1978
    ..K., from whom copies may be obtained under the terms given in Biochem. J. (1978) 169, 5...
  71. ncbi Steady-state fluorescence of Escherichia coli phosphofructokinase reveals a regulatory role for ATP
    S A Berger
    MRC Laboratory of Molecular Biology, Cambridge, England
    Biochemistry 30:8477-80. 1991
    ..coli PFK exists in a state with high affinity for Fru6P ("R" state). This state can be altered to a low affinity ("T" state) by PEP binding to the allosteric site or by ATP binding to the enzyme...
  72. ncbi Glucokinase and glucokinase regulatory proteins are functionally coexpressed before birth in the rat brain
    I Roncero
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, Complutense University, Madrid, Spain
    J Neuroendocrinol 21:973-81. 2009
    ..Our findings indicate that both proteins may be functionally active before birth and that they can act within a glucose sensor system involved in controlling food intake...
  73. ncbi Study of hereditary fructose intolerance by use of 31P magnetic resonance spectroscopy
    R D Oberhaensli
    MRC Clinical Magnetic Resonance Facility, John Radcliffe Hospital, Oxford
    Lancet 2:931-4. 1987
    ..The effect of fructose on liver Pi and plasma urate was most pronounced in heterozygotes with gout (n = 3). Heterozygosity for HFI may predispose to hyperuricaemia...
  74. ncbi Substrate antagonism in the kinetic mechanism of E. coli phosphofructokinase-1
    D Deville-Bonne
    Laboratoire d Enzymologie du CNRS, Gif sur Yvette, France
    FEBS Lett 290:173-6. 1991
    ..The antagonism between ATP and fructose-6-phosphate shows that a negative interaction occurs during the reaction with E. coli phosphofructokinase-1 which must be considered in addition to its allosteric properties...
  75. pmc Underestimation of the pentose-phosphate pathway in intact primary neurons as revealed by metabolic flux analysis
    Patricia Rodriguez-Rodriguez
    Institute of Functional Biology and Genomics IBFG, University of Salamanca CSIC, Salamanca, Spain
    J Cereb Blood Flow Metab 33:1843-5. 2013
    ..8-fold the PPP rate. Thus, in neurons, a considerable fraction of fructose-6-phosphate returning from the PPP contributes to the G6P pool that re-enters PPP, largely underestimating its flux. ..
  76. pmc The ancestral activation promiscuity of ADP-glucose pyrophosphorylases from oxygenic photosynthetic organisms
    Misty L Kuhn
    Department of Chemistry and Biochemistry, Loyola University Chicago, 1032 W, Sheridan Rd, Chicago, IL 60660, USA
    BMC Evol Biol 13:51. 2013
    ....
  77. pmc Structure-based development of small molecule PFKFB3 inhibitors: a framework for potential cancer therapeutic agents targeting the Warburg effect
    Minsuh Seo
    Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, United States of America
    PLoS ONE 6:e24179. 2011
    ..This study validates PFKFB3 as a target for new cancer therapies and provides a framework for future development efforts...
  78. ncbi Changes in the contents of metabolites and enzyme activities in rice plants responding to Rhizoctonia solani Kuhn infection: activation of glycolysis and connection to phenylpropanoid pathway
    J Musembi Mutuku
    Saga University, Faculty of Agriculture, 1 Honjo Machi, Saga City, 840 8502 Japan
    Plant Cell Physiol 53:1017-32. 2012
    ....
  79. pmc Resolving the sources of plasma glucose excursions following a glucose tolerance test in the rat with deuterated water and [U-13C]glucose
    Teresa C Delgado
    Intermediary Metabolism Group, Center for Neurosciences and Cell Biology, Coimbra, Portugal
    PLoS ONE 7:e34042. 2012
    ..In rats that were placed on high-fat diet, the development of glucose intolerance was associated with a significantly higher gluconeogenic contribution to plasma glucose levels after the load...
  80. ncbi Anomeric specificity of D-glucose production by rat hepatocytes
    W J Malaisse
    Laboratory of Experimental Hormonology ULB and High Resolution NMR Centre VUB, Brussels Free Universities, Brussels, Belgium
    Mol Cell Biochem 266:145-50. 2004
    ....
  81. ncbi Molecular cloning and characterization of Plasmodium falciparum transketolase
    Shweta Joshi
    Division of Biochemistry, Central Drug Research Institute, Lucknow 226001, Uttar Pradesh, India
    Mol Biochem Parasitol 160:32-41. 2008
    ..The availability of a structural model of PfTk and the observed differences in its kinetic properties compared to the host enzyme may facilitate designing of novel inhibitors of PfTk with potential anti-malarial activity...
  82. pmc The glycosylation pathway of Eimeria tenella is upregulated during gametocyte development and may play a role in oocyst wall formation
    Robert A Walker
    Institute for the Biotechnology of Infectious Diseases, University of Technology, Sydney, New South Wales 2007, Australia
    Eukaryot Cell 9:127-35. 2010
    ..tenella sexual stages and may play a role in directing a number of macrogamete proteins to the developing oocyst wall...
  83. ncbi Endogenous effectors of human liver glycogen phosphorylase modulate effects of indole-site inhibitors
    Nacide Ercan-Fang
    Metabolic Research Laboratory, Section of Endocrinology, Metabolism, and Nutrition, Minneapolis Veterans Affairs Medical Center, Minneapolis, MN 55417, USA
    Am J Physiol Endocrinol Metab 289:E366-72. 2005
    ..However, at higher concentrations (10-30 microM), the GPI effect was dominant and resulted in inhibition of phosphorylase a activity irrespective of the presence or absence of the other modulators of the enzyme...
  84. ncbi Noninvasive analysis of hepatic glycogen kinetics before and after breakfast with deuterated water and acetaminophen
    John G Jones
    NMR Research Unit, Department of Biochemistry and Center for Neurosciences and Cell Biology, Faculty of Sciences and Technology, University of Coimbra, 3001 401 Coimbra, Portugal
    Diabetes 55:2294-300. 2006
    ..Unlike current measurements of human hepatic glycogen metabolism, the (2)H(2)O/acetaminophen assay does not require specialized on-site clinical equipment or personnel...
  85. ncbi The crystal structure of ATP-bound phosphofructokinase from Trypanosoma brucei reveals conformational transitions different from those of other phosphofructokinases
    Iain W McNae
    Structural Biochemistry Group, Institute of Structural and Molecular Biology, University of Edinburgh, King s Buildings, Edinburgh, Scotland
    J Mol Biol 385:1519-33. 2009
    ..brucei PFK compared with bacterial and mammalian PFKs give optimism for the discovery of species-specific drugs for the treatment of diseases caused by protist parasites of the trypanosomatid family...
  86. ncbi Gene cloning and catalysis features of a new mannitol-1-phosphate dehydrogenase (BbMPD) from Beauveria bassiana
    Zheng Liang Wang
    Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, People s Republic of China
    Carbohydr Res 345:50-4. 2010
    ..0 and was much more sensitive to Cu(2+) and Zn(2+) than to Li(+) and Mn(2+). The results indicate a crucial role of BbMPD in the mannitol biosynthesis of B. bassiana...
  87. ncbi Strain and near attack conformers in enzymic thiamin catalysis: X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor aldo
    Peter Asztalos
    Institut für Biochemie Biotechnologie, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06120 Halle Saale, Germany
    Biochemistry 46:12037-52. 2007
    ....
  88. pmc Localization of phosphoglucose isomerase in Escherichia coli and its relation to the induction of the hexose phosphate transport system
    I Friedberg
    J Bacteriol 112:1201-5. 1972
    ..S6P was not taken by intact cells. The data indicate that the isomerization of F6P to G6P can take place on the cell surface, and this explains the indirect induction of hexose phosphate transport by F6P...
  89. ncbi Clotrimazole potentiates the inhibitory effects of ATP on the key glycolytic enzyme 6-phosphofructo-1-kinase
    Mariah Celestino Marcondes
    Laboratório de Oncobiologia Molecular LabOMol and Laboratório de Enzimologia e Controle do Metabolismo LabECoM, Departamento de Fármacos, Faculdade de Farmacia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941 590, Brazil
    Arch Biochem Biophys 497:62-7. 2010
    ..Altogether, the results presented here suggest that PFK inhibition by CTZ involves a decrease in the affinity of PFK for its substrates at the catalytic site with the concomitant potentiation of the inhibitory properties of ATP...
  90. ncbi Fructose 1,6-bisphosphate aldolase/phosphatase may be an ancestral gluconeogenic enzyme
    Rafael F Say
    Mikrobiologie, Fakultat Biologie, Universitat Freiburg, Schanzlestrasse 1, D 79104 Freiburg, Germany
    Nature 464:1077-81. 2010
    ..We propose that this highly conserved, heat-stabile and bifunctional FBP aldolase/phosphatase represents the pace-making ancestral gluconeogenic enzyme, and that in evolution gluconeogenesis preceded glycolysis...
  91. ncbi Unfolding pathway of the dimeric and tetrameric forms of phosphofructokinase-2 from Escherichia coli
    Mauricio Baez
    Departamento de Biologia, Facultad de Ciencias Universidad de Chile, Casilla 653, Santiago, Chile
    Biochemistry 46:6141-8. 2007
    ....
  92. ncbi Expression and purification of active human internal His(6)-tagged L-glutamine: D-Fructose-6P amidotransferase I
    Céline Richez
    Institut de Chimie des Substances Naturelles, ICSN CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Protein Expr Purif 54:45-53. 2007
    ..The stimulation of hydrolysis of the alternate substrate L-glutamine para-nitroanilide by D-fructose-6P (Fru-6P) afforded a K(d) of 5 microM for Fru-6P...
  93. pmc The crystal complex of phosphofructokinase-2 of Escherichia coli with fructose-6-phosphate: kinetic and structural analysis of the allosteric ATP inhibition
    Ricardo Cabrera
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
    J Biol Chem 286:5774-83. 2011
    ..Differences and resemblances in the patterns of fructose-6-P binding and the mechanism of inhibition are discussed for Pfk-1 and Pfk-2, as an example of evolutionary convergence, because these enzymes do not share a common ancestor...
  94. pmc Glucose-6-phosphate isomerase deficiency results in mTOR activation, failed translocation of lipin 1α to the nucleus and hypersensitivity to glucose: Implications for the inherited glycolytic disease
    Jorge F Haller
    Department of Physiology and Biophysics, Boston University School of Medicine, USA
    Biochim Biophys Acta 1812:1393-402. 2011
    ..These results shed light on the mechanism underlying the pathologies associated with inherited GPI deficiency and the variability in the severity of the symptoms observed in these patients...
  95. ncbi Revised procedures for yeast metabolites extraction: application to a glucose pulse to carbon-limited yeast cultures, which reveals a transient activation of the purine salvage pathway
    Marie Odile Loret
    Laboratoire de Biotechnologie et Bioprocédés, UMR CNRS 5504, UMR INRA 792, Avenue de Rangueil, 31077 Toulouse Cedex 04, France
    Yeast 24:47-60. 2007
    ..This metabolic perturbation was accompanied by a rapid increase in the activity of the ISN1-encoded specific IMP-5'-nucleotidase. The mechanism of this activation remains to be determined...
  96. ncbi Evidence for impaired gluconeogenesis in very long-chain acyl-CoA dehydrogenase-deficient mice
    U Spiekerkoetter
    Department of General Pediatrics, University Children s Hospital, Moorenstrasse 5, 40225 Dusseldorf, Germany
    Horm Metab Res 38:625-30. 2006
    ..Whether this is due to lack of a substrate, inhibitory effects on other gluconeogenic enzymes or impaired transcription of gluconeogenic enzymes needs to be resolved in the future...
  97. ncbi Methylglyoxal is an intermediate in the biosynthesis of 6-deoxy-5-ketofructose-1-phosphate: a precursor for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii
    Robert H White
    Department of Biochemistry 0308, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA
    Biochemistry 45:12366-79. 2006
    ..This work is the first report of methylglyoxal functioning in central metabolism...
  98. ncbi Characterization of hyperthermostable fructose-1,6-bisphosphatase from Thermococcus onnurineus NA1
    Yeol Gyun Lee
    Division of Life Science, Korea Basic Science Institute, Daejeon, 305 806, Republic of Korea
    J Microbiol 48:803-7. 2010
    ..However, this enzyme shows a different range of substrate specificities. These results suggest that TNA1-Fbp can establish new criterion for class V FBPases...
  99. ncbi Simultaneous quantification of metabolites involved in central carbon and energy metabolism using reversed-phase liquid chromatography-mass spectrometry and in vitro 13C labeling
    Wen Chu Yang
    Bindley Bioscience Center, Purdue University, West Lafayette, Indiana 47907, USA
    Anal Chem 80:9508-16. 2008
    ..Thirty common metabolites involved in glycolysis, the pentose phosphate pathway, and tricarboxylic acid cycle were identified and quantified from yeast lysate with a relative standard deviation of less than 10%...
  100. ncbi Structure of a ternary complex of an allosteric lactate dehydrogenase from Bacillus stearothermophilus at 2.5 A resolution
    D B Wigley
    Department of Biochemistry, University of Leicester, U K
    J Mol Biol 223:317-35. 1992
    ..His188 is connected to His195 (which binds the carbonyl group of the substrate) and Arg173 is connected to Arg171 (the residue that binds the carboxylate group of the substrate)...
  101. pmc Hepatic intralobular mapping of fructose metabolism in the rat liver
    S P Burns
    Cell Regulation Unit, Department of Diabetes and Metabolic Medicine, 5th Floor Alexandra Wing, St Bartholomew s and the Royal London School of Medicine and Dentistry, Whitechapel Road, London E1 1BB, UK
    Biochem J 349:539-45. 2000
    ..It may be inferred that fructokinase activity is expressed throughout the hepatic lobule...