raffinose

Summary

Summary: A trisaccharide occurring in Australian manna (from Eucalyptus spp, Myrtaceae) and in cottonseed meal.

Top Publications

  1. pmc Galactinol and raffinose constitute a novel function to protect plants from oxidative damage
    Ayako Nishizawa
    Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, 3327 204 Nakamachi, Nara 631 8505, Japan
    Plant Physiol 147:1251-63. 2008
  2. doi A genetic element present on megaplasmids allows Enterococcus faecium to use raffinose as carbon source
    Xinglin Zhang
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    Environ Microbiol 13:518-28. 2011
  3. pmc Regulation of the alpha-galactosidase activity in Streptococcus pneumoniae: characterization of the raffinose utilization system
    C Rosenow
    Versicor, Inc, Fremont, California 94555, USA
    Genome Res 9:1189-97. 1999
  4. ncbi Apoptosis versus necrosis during cold storage and rewarming of human renal proximal tubular cells
    A K Salahudeen
    Department of Medicine, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216-4505, USA
    Transplantation 72:798-804. 2001
  5. ncbi Mechanism and prevention of cold storage-induced human renal tubular cell injury
    A K Salahudeen
    Department of Medicine, University of Mississippi Medical Center, Jackson 39216 4505, USA
    Transplantation 70:1424-31. 2000
  6. doi Proteomic analysis of common bean seed with storage protein deficiency reveals up-regulation of sulfur-rich proteins and starch and raffinose metabolic enzymes, and down-regulation of the secretory pathway
    Frédéric Marsolais
    Southern Crop Protection and Food Research Centre, Agriculture and Agri Food Canada, London, ON, Canada
    J Proteomics 73:1587-600. 2010
  7. pmc Fructans, but not the sucrosyl-galactosides, raffinose and loliose, are affected by drought stress in perennial ryegrass
    Véronique Amiard
    Unité Mixte de Recherche Institut National de la Recherche Agronomique Université de Caen Basse Normandie, Laboratoire de Physiologie et Biochimie Végétales, Institut de Recherche en Biologie Appliquée, Universite, 14032 Caen Cedex, France
    Plant Physiol 132:2218-29. 2003
  8. ncbi Protection mechanisms in the resurrection plant Xerophyta viscosa (Baker): both sucrose and raffinose family oligosaccharides (RFOs) accumulate in leaves in response to water deficit
    Shaun Peters
    University of Cape Town, Department of Molecular and Cell Biology, Private Bag, Rhodes Gift, 7701, Cape Town, South Africa
    J Exp Bot 58:1947-56. 2007
  9. doi Impact of pancreatic cold preservation on rat islet recovery and function
    Antonello Pileggi
    Cell Transplant Center, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 33136, USA
    Transplantation 87:1442-50. 2009
  10. doi Functional identification of Arabidopsis ATSIP2 (At3g57520) as an alkaline α-galactosidase with a substrate specificity for raffinose and an apparent sink-specific expression pattern
    Shaun Peters
    Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, 8008 Zurich, Switzerland
    Plant Cell Physiol 51:1815-9. 2010

Detail Information

Publications237 found, 100 shown here

  1. pmc Galactinol and raffinose constitute a novel function to protect plants from oxidative damage
    Ayako Nishizawa
    Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, 3327 204 Nakamachi, Nara 631 8505, Japan
    Plant Physiol 147:1251-63. 2008
    Galactinol synthase (GolS) is a key enzyme in the synthesis of raffinose family oligosaccharides that function as osmoprotectants in plant cells...
  2. doi A genetic element present on megaplasmids allows Enterococcus faecium to use raffinose as carbon source
    Xinglin Zhang
    Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands
    Environ Microbiol 13:518-28. 2011
    ..faecium gene cluster that is responsible for the metabolism of the α-galactoside sugar raffinose. Phenotypic testing of seven E...
  3. pmc Regulation of the alpha-galactosidase activity in Streptococcus pneumoniae: characterization of the raffinose utilization system
    C Rosenow
    Versicor, Inc, Fremont, California 94555, USA
    Genome Res 9:1189-97. 1999
    ..the Streptococcus pneumoniae genome sequence that contains eight genes involved in regulation and metabolism of raffinose. The genes rafR and rafS are transcribed as one operon, and their gene products regulate the raffinose-dependent ..
  4. ncbi Apoptosis versus necrosis during cold storage and rewarming of human renal proximal tubular cells
    A K Salahudeen
    Department of Medicine, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216-4505, USA
    Transplantation 72:798-804. 2001
    ..Inclusion of antioxidants in the storage solution confers protection against both cold storage and rewarming-induced necrosis and apoptosis...
  5. ncbi Mechanism and prevention of cold storage-induced human renal tubular cell injury
    A K Salahudeen
    Department of Medicine, University of Mississippi Medical Center, Jackson 39216 4505, USA
    Transplantation 70:1424-31. 2000
    ..The objective was, therefore, to seek direct evidence for the free radical production during the cold storage of human renal tubular cells, and to define the roles of extrinsic and intrinsic antioxidants in cold-induced cell injury...
  6. doi Proteomic analysis of common bean seed with storage protein deficiency reveals up-regulation of sulfur-rich proteins and starch and raffinose metabolic enzymes, and down-regulation of the secretory pathway
    Frédéric Marsolais
    Southern Crop Protection and Food Research Centre, Agriculture and Agri Food Canada, London, ON, Canada
    J Proteomics 73:1587-600. 2010
    ..starch composition, whereas increased levels of UDP-glucose 4-epimerase were correlated with a 30% increase in raffinose content. Induction of cell division cycle protein 48 and ubiquitin suggested enhanced ER-associated degradation...
  7. pmc Fructans, but not the sucrosyl-galactosides, raffinose and loliose, are affected by drought stress in perennial ryegrass
    Véronique Amiard
    Unité Mixte de Recherche Institut National de la Recherche Agronomique Université de Caen Basse Normandie, Laboratoire de Physiologie et Biochimie Végétales, Institut de Recherche en Biologie Appliquée, Universite, 14032 Caen Cedex, France
    Plant Physiol 132:2218-29. 2003
    ..the putative role of the sucrosyl-galactosides, loliose [alpha-D-Gal (1,3) alpha-D-Glc (1,2) beta-D-Fru] and raffinose [alpha-D-Gal (1,6) alpha-D-Glc (1,2) beta-D-Fru], in drought tolerance of perennial ryegrass and to compare it ..
  8. ncbi Protection mechanisms in the resurrection plant Xerophyta viscosa (Baker): both sucrose and raffinose family oligosaccharides (RFOs) accumulate in leaves in response to water deficit
    Shaun Peters
    University of Cape Town, Department of Molecular and Cell Biology, Private Bag, Rhodes Gift, 7701, Cape Town, South Africa
    J Exp Bot 58:1947-56. 2007
    ..Sucrose and raffinose family oligosaccharides (RFOs), particularly raffinose, increased under these conditions, with the highest ..
  9. doi Impact of pancreatic cold preservation on rat islet recovery and function
    Antonello Pileggi
    Cell Transplant Center, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 33136, USA
    Transplantation 87:1442-50. 2009
    ..Islet transplantation success depends on the number and quality of islets transplanted. This study aimed at exploring the molecular mechanisms associated with cold pancreas preservation and their impact on islet cell survival and function...
  10. doi Functional identification of Arabidopsis ATSIP2 (At3g57520) as an alkaline α-galactosidase with a substrate specificity for raffinose and an apparent sink-specific expression pattern
    Shaun Peters
    Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, 8008 Zurich, Switzerland
    Plant Cell Physiol 51:1815-9. 2010
    Arabidopsis ATSIP2 has recently been suggested to be a raffinose synthase gene. However, it has high amino acid identity to functionally characterized alkaline α-galactosidases from Cucumis melo and Zea mays...
  11. ncbi Important roles of drought- and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana
    Teruaki Taji
    Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
    Plant J 29:417-26. 2002
    b>Raffinose family oligosaccharides (RFO) accumulating during seed development are thought to play a role in the desiccation tolerance of seeds. However, the functions of RFO in desiccation tolerance have not been elucidated...
  12. doi Raffinose in chloroplasts is synthesized in the cytosol and transported across the chloroplast envelope
    Thomas Schneider
    Molekulare Pflanzenphysiologie, Institut fur Pflanzenbiologie, Universitat Zurich, Zollikerstrasse 107, 8008 Zurich, Switzerland
    Plant Cell Physiol 50:2174-82. 2009
    ..The trisaccharide raffinose (alpha-1,6-galactosyl sucrose) is such a carbohydrate but has received little attention...
  13. doi Expression of three galactinol synthase isoforms in Coffea arabica L. and accumulation of raffinose and stachyose in response to abiotic stresses
    Tiago B Dos Santos
    Instituto Agronômico do Paraná IAPAR, Laboratório de Biotecnologia Vegetal, Londrina PR, Brazil
    Plant Physiol Biochem 49:441-8. 2011
    Galactinol synthase (EC 2.4.1.123; GolS) catalyzes the first step in the synthesis of raffinose family oligosaccharides (RFOs). Their accumulation in response to abiotic stresses implies a role for RFOs in stress adaptation...
  14. pmc Role of dihydrolipoamide dehydrogenase in regulation of raffinose transport in Streptococcus pneumoniae
    Robert E Tyx
    Department of Microbiology and Immunology, University at Buffalo, State University of New York, Buffalo, New York 14214, USA
    J Bacteriol 193:3512-24. 2011
    ..strains lacking the enzyme dihydrolipoamide dehydrogenase (DLDH) show markedly reduced ability to grow on raffinose and stachyose as sole carbon sources...
  15. doi Levansucrase and sucrose phoshorylase contribute to raffinose, stachyose, and verbascose metabolism by lactobacilli
    Januana S Teixeira
    University of Alberta, Department of Agricultural, Food and Nutritional Science, Edmonton, Canada
    Food Microbiol 31:278-84. 2012
    b>Raffinose family oligosaccharides (RFOs) in food are considered anti-nutritional factors. This study elucidated the role of α-galactosidase (α-Gal), levansucrase, and sucrose phosphorylase for conversion of RFOs by lactobacilli...
  16. ncbi Accumulation of crystal deposits in abdominal organs following perfusion with defrosted University of Wisconsin solutions
    Stefan G Tullius
    Department of Surgery, Charite Virchow Clinic, Berlin, Germany
    Am J Transplant 2:627-30. 2002
    ..UW solutions can reach subzero temperatures under commonly used transportation conditions. Under these conditions, visible crystals accumulate and can be detected in abdominal organs of an experimental system...
  17. ncbi Cold induces catalytic iron release of cytochrome P-450 origin: a critical step in cold storage-induced renal injury
    Hong Huang
    Department of Medicine, University of Mississippi Medical Center, Jackson 39216 4505, USA
    Am J Transplant 2:631-9. 2002
    ..In the clinical setting, sequestering free iron released during cold storage is possible and may prove to be useful in limiting organ injury...
  18. ncbi Cold-induced apoptosis of rat liver cells in University of Wisconsin solution: the central role of chelatable iron
    Uta Kerkweg
    Institut fur Physiologische Chemie, Universitatsklinikum, Hufelandstrasse 55, D 45122 Essen, Germany
    Hepatology 35:560-7. 2002
    ..With regard to the extent of this iron-mediated injury, addition of a suitable iron chelator to UW solution might markedly improve the outcome of liver preservation...
  19. ncbi Addition of adenosine monophosphate-activated protein kinase activators to University of Wisconsin solution: a way of protecting rat steatotic livers
    Ismail Ben Mosbah
    Experimental Hepatic Ischemia Reperfusion Unit, Instituto de Investigaciones Biomédicas de Barcelona, Instituto de Investigaciones Biomédicas de Barcelona Consejo Superior de Investigaciones Científicas, Spain
    Liver Transpl 13:410-25. 2007
    ..TMZ and AICAR may constitute new additives to UW solution in steatotic liver preservation, whereas a combination of both seems unnecessary...
  20. ncbi Improved rat liver preservation by hypothermic continuous machine perfusion using polysol, a new, enriched preservation solution
    Maud Bessems
    Surgical Laboratory, Department of Surgery, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Liver Transpl 11:539-46. 2005
    ..In conclusion, machine perfusion using Polysol results in better quality liver preservation than cold storage with UW and machine perfusion using UW-G...
  21. doi Transcriptome profiles of hybrid poplar (Populus trichocarpa × deltoides) reveal rapid changes in undamaged, systemic sink leaves after simulated feeding by forest tent caterpillar (Malacosoma disstria)
    Ryan N Philippe
    Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada
    New Phytol 188:787-802. 2010
    ..Induced changes of galactinol and raffinose oligosaccharides were detected by anion-exchange high-pressure liquid chromatography...
  22. doi Improvement of the cold storage of blood vessels with a vascular preservation solution. Study in porcine aortic segments
    Timo Wille
    Institut fur Physiologische Chemie, Universitatsklinikum, Essen, Germany
    J Vasc Surg 47:422-31. 2008
    ..Hypothesizing that this iron-dependent cold-induced injury might also occur in the endothelium of intact vessels, we here set out to optimize the hypothermic storage of blood vessels...
  23. ncbi Perfluorocarbon for organ preservation before transplantation
    Shinichi Matsumoto
    Islet and Cell Processing Laboratory, Puget Sound Blood Center Northwest Tissue Center, Seattle, WA, USA
    Transplantation 74:1804-9. 2002
    ..The TLM has also been shown to be beneficial for preserving other difficult organs, such as heart and small bowel...
  24. ncbi Hypothermia causes a marked injury to rat proximal tubular cells that is aggravated by all currently used preservation solutions
    Miranda Bartels-Stringer
    Department of Pharmacology and Toxicology, University Medical Center Nijmegen, Nijmegen, The Netherlands
    Cryobiology 47:82-91. 2003
    ..Iron-dependent MPT is suggested to play a role in this damage. Strategies to prevent cold-induced injury should aim at reducing the availability of "free" iron...
  25. pmc Analysis of the raffinose family oligosaccharide pathway in pea seeds with contrasting carbohydrate composition
    T Peterbauer
    Chemical Physiology of Plants, Institute of Ecology, University of Vienna, A-1090 Vienna, Austria
    Plant Physiol 127:1764-72. 2001
    b>Raffinose family oligosaccharides (RFOs) are synthesized by a set of galactosyltransferases, which sequentially add galactose units from galactinol to sucrose...
  26. ncbi Effect of sucrose/raffinose mass ratios on the stability of co-lyophilized protein during storage above the Tg
    P Davidson
    Department of Biological Sciences, National University of Singapore, Singapore
    Pharm Res 18:474-9. 2001
    PURPOSE: To examine the potential of raffinose as an excipient in stabilizing protein and to study the effect of sucrose/raffinose mass ratios on the stability of co-lyophilized protein and amorphous solids during storage at an elevated ..
  27. pmc Modulation of rat cecal microbiota by administration of raffinose and encapsulated Bifidobacterium breve
    Achmad Dinoto
    Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita ku, Sapporo, Hokkaido 060 8589, Japan
    Appl Environ Microbiol 72:784-92. 2006
    To investigate the effects of administration of raffinose and encapsulated Bifidobacterium breve JCM 1192T cells on the rat cecal microbiota, in a preclinical synbiotic study groups of male WKAH/Hkm Slc rats were fed for 3 weeks with ..
  28. doi In vitro fermentation of alternansucrase raffinose-derived oligosaccharides by human gut bacteria
    Oswaldo Hernández-Hernández
    Instituto de Química Orgánica general CSIC, Juan de la Cierva 3, 28006 Madrid, Spain
    J Agric Food Chem 59:10901-6. 2011
    In this work, in vitro fermentation of alternansucrase raffinose-derived oligosaccharides, previously fractionated according to their degree of polymerization (DP; from DP4 to DP10), was carried out using small-scale pH-controlled batch ..
  29. ncbi Factors affecting human islet of Langerhans isolation yields
    C Toso
    Division of Surgical Research, Department of Surgery, University Hospital, University of Geneva, Switzerland
    Transplant Proc 34:826-7. 2002
  30. doi Raffinose and stachyose metabolism are not required for efficient soybean seed germination
    Emily C Dierking
    University of Missouri Columbia, Division of Plant Sciences, 110 Waters Hall, Columbia, MO 65211, USA
    J Plant Physiol 166:1329-35. 2009
    b>Raffinose family oligosaccharides (RFOs), which include raffinose and stachyose, are thought to be an important source of energy during seed germination...
  31. ncbi Experimental small bowel preservation using Polysol: a new alternative to University of Wisconsin solution, Celsior and histidine-tryptophan-ketoglutarate solution?
    Lai Wei
    House of Experimental Therapy, University of Bonn, Sigmund Freud Strasse 25, 53105 Bonn, Germany
    World J Gastroenterol 13:3684-91. 2007
    ....
  32. doi Expression of a GALACTINOL SYNTHASE gene is positively associated with desiccation tolerance of Brassica napus seeds during development
    Xu Li
    National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing, China
    J Plant Physiol 168:1761-70. 2011
    Desiccation tolerance of seeds is positively correlated with raffinose family oligosaccharides (RFOs). However, RFOs' role in desiccation tolerance is still a matter of controversy...
  33. pmc Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation
    Yosuke Hirayama
    Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan
    Appl Environ Microbiol 78:4984-94. 2012
    ..Markerless gene deletion was tested using the aga gene, which encodes α-galactosidase, whose substrates include raffinose. Almost all the pTBR101-CM-transformed strains became double-crossover recombinants after subculture, and 4 out ..
  34. ncbi Inherent toxicity of organ preservation solutions to cultured hepatocytes
    Ursula Rauen
    Institut fur Physiologische Chemie, Universitatsklinikum, Hufelandstr 55, D 45122 Essen, Germany
    Cryobiology 56:88-92. 2008
    ..These potential toxicities should be taken into account in the development of future preservation solutions...
  35. ncbi Degradation of raffinose oligosaccharides in soymilk by immobilized alpha-galactosidase of Aspergillus oryzae
    Girigowda Kotiguda
    Department of Biochemistry, Gulbarga University, Gulbarga 585106, Karnataka, India
    J Microbiol Biotechnol 17:1430-6. 2007
    ..The immobilized enzyme was used in batch, repeated batch, and continuous modes to degrade the raffinose-family sugars present in soymilk...
  36. doi Diets supplemented with chickpea or its main oligosaccharide component raffinose modify faecal microbial composition in healthy adults
    W M U Fernando
    Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Canada
    Benef Microbes 1:197-207. 2010
    The effects of diets supplemented with either chickpea or its main oligosaccharide raffinose on the composition of the faecal microbial community were examined in 12 healthy adults (18-65 years) in a randomised crossover intervention ..
  37. doi Genome-wide identification of genes involved in raffinose metabolism in Maize
    Mei Liang Zhou
    School of Life and Basic Sciences, Sichuan Agricultural University, Yaan, Sichuan 625014, People s Republic of China
    Glycobiology 22:1775-85. 2012
    The raffinose family oligosaccharides (RFOs), such as raffinose and stachyose, are synthesized by a set of distinct galactosyltransferases, which sequentially add galactose units to sucrose...
  38. ncbi Cold preservation-warm reperfusion perturbs cytosolic calcium ion homeostasis in rat liver sinusoidal endothelial cells
    Stéphanie Auger
    Department of Pharmacology, Universite de Montreal, Quebec, Canada
    Liver Transpl 9:150-9. 2003
    ..Cold preservation does not significantly affect changes in SEC steady-state Ca(2+) levels, but greatly impairs the capacity of SECs to subsequently respond to Ca(2+)-mobilizing agonists...
  39. doi Static cold storage preservation of ischemically damaged kidneys. a comparison between IGL-1 and UW solution
    Mark Hugo J Maathuis
    University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    Transpl Int 21:473-82. 2008
    ..In the present experiment, static CS preservation of ischemically damaged rat kidneys in either IGL-1 or UW-CSS rendered equal results after transplantation...
  40. ncbi Role of perfusion medium, oxygen and rheology for endoplasmic reticulum stress-induced cell death after hypothermic machine preservation of the liver
    Steffen Manekeller
    Surgical Research Division, University Clinic of Surgery, Bonn, Germany
    Transpl Int 21:169-77. 2008
    ..The simple insufflation of gaseous O2 may be considered a feasible alternative, apparently indifferent to the endoplasmic reticulum...
  41. ncbi Effects of polyethylene glycol and hydroxyethyl starch in University of Wisconsin preservation solution on human red blood cell aggregation and viscosity
    I B Mosbah
    Liver Ischemia Reperfusion Injury Unit, the Instituto de Investigaciones Biomédicas de Barcelona, CSIC IDIBAPS, Barcelona, Spain
    Transplant Proc 38:1229-35. 2006
    ..These results were consistent with reduced viscosity for PEG-containing solutions. In conclusion, the use of PEG did not change the physiologic function of human RBCs and thus may be an alternative to HES in UW liquids...
  42. doi Polyethylene glycol modified-albumin enhances the cold preservation properties of University of Wisconsin solution in rat liver and a hepatocyte cell line
    Rime Abbas
    Department of Surgery, University Hospitals Case Medical Center, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 5047, USA
    J Surg Res 164:95-104. 2010
    ..Peg-Alb-UW solution improved the integrity of the glutathione redox buffer system of a hepatocyte cell line after cold storage and reperfusion...
  43. ncbi Effect of University of Wisconsin organ-preservation solution on haemorheology
    Arjan van der Plaats
    Department of Biomedical Engineering, University of Groningen, P O Box 196, 9700 AD Groningen, The Netherlands
    Transpl Int 17:227-33. 2004
    ..The aggregates are larger than the diameter of the sinusoids. Initial washout could be optimised by pre-flushing to improve the viability of the liver and to decrease delayed graft function...
  44. ncbi Improved islet yield and function with ductal injection of University of Wisconsin solution before pancreas preservation
    Toshiya Sawada
    Diabetes Institute for Immunology and Transplantation, Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA
    Transplantation 75:1965-9. 2003
    ....
  45. pmc alpha-Galactoside uptake in Rhizobium meliloti: isolation and characterization of agpA, a gene encoding a periplasmic binding protein required for melibiose and raffinose utilization
    D J Gage
    Department of Biological Sciences, Howard Hughes Medical Institute, Stanford University, Stanford, California 94305 5020, USA
    J Bacteriol 180:5739-48. 1998
    ..meliloti and at low levels in the free-living bacteria, we propose that AgpA may belong to the class of gene products whose expression decreases when R. meliloti becomes an intracellular symbiont...
  46. ncbi Two-layer method (UW solution/perfluorochemical plus O2) for lung preservation in rat lung transplantation
    C C Liu
    Taipei Venteran General Hospital, Taipei, Taiwan
    Transplant Proc 39:3019-23. 2007
    ..Histologic examination revealed more inflammatory cell aggregates in the lung graft preserved using a two-layer method...
  47. ncbi Preservation of the human pancreas before islet isolation using a two-layer (UW solution-perfluorochemical) cold storage method
    Jonathan R T Lakey
    Department of Surgery, Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 74:1809-11. 2002
  48. ncbi Modified two-layer preservation method (M-Kyoto/PFC) improves islet yields in islet isolation
    H Noguchi
    Diabetes Research Institute Kyoto, Kyoto University Graduate School of Medicine, 54 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Am J Transplant 6:496-504. 2006
    ..In conclusion, the advantages of M-Kyoto solution are trypsin inhibition and less collagenase inhibition. Based on these data, we now use M-Kyoto solution for clinical islet transplantation from nonheart-beating donor pancreata...
  49. ncbi Molecular markers in stored kidneys using perfluorocarbon-based preservation solution: preliminary results
    D G Maluf
    Division of Transplant, Department of Surgery, Virginia Commonwealth University, Richmond, Virginia 23298 0248, USA
    Transplant Proc 38:1243-6. 2006
    ..We evaluated histologically and a number of molecular changes induced by ischemia in stored kidneys with University of Wisconsin (UW) and PFC-based preservation solutions (PFC-UW)...
  50. ncbi Chain Elongation of raffinose in pea seeds. Isolation, characterization, and molecular cloning of mutifunctional enzyme catalyzing the synthesis of stachyose and verbascose
    Thomas Peterbauer
    Institute of Ecology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria
    J Biol Chem 277:194-200. 2002
    b>Raffinose oligosaccharides are major soluble carbohydrates in seeds and other tissues of plants...
  51. ncbi Over-expression of OsUGE-1 altered raffinose level and tolerance to abiotic stress but not morphology in Arabidopsis
    Hui Li Liu
    Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Nanxincun 20, Xiangshan, Beijing 100093, China
    J Plant Physiol 164:1384-90. 2007
    ..In addition, transgenic plants showed a higher level of the soluble sugar raffinose than did wild-type plants...
  52. ncbi Ductal injection of preservation solution increases islet yields in islet isolation and improves islet graft function
    Hirofumi Noguchi
    Transplantation Unit, Kyoto University Hospital, Kyoto 606 8507, Japan
    Cell Transplant 17:69-81. 2008
    ..Based on these data, this technique is now used for clinical islet transplantation from non-heart-beating donor pancreata or living donor pancreas...
  53. pmc Mrt, a gene unique to fungi, encodes an oligosaccharide transporter and facilitates rhizosphere competency in Metarhizium robertsii
    Weiguo Fang
    Department of Entomology, University of Maryland, College Park, Maryland 20742, USA
    Plant Physiol 154:1549-57. 2010
    ..Here, we demonstrate that sucrose and raffinose family oligosaccharides in root exudates are important for rhizosphere competence in the insect pathogen ..
  54. ncbi myo-Inositol and sucrose concentrations affect the accumulation of raffinose family oligosaccharides in seeds
    Ute Karner
    Institute of Ecology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria
    J Exp Bot 55:1981-7. 2004
    b>Raffinose family oligosaccharides (RFOs) fulfil multiple functions in plants. In seeds, they possibly protect cellular structures during desiccation and constitute carbon reserves for early germination...
  55. ncbi Inhibition of raffinose oligosaccharide breakdown delays germination of pea seeds
    Andreas Blöchl
    Department of Chemical Ecology and Ecosystem Research, University of Vienna, Althanstr 14, A 1090 Vienna, Austria
    J Plant Physiol 164:1093-6. 2007
    b>Raffinose family oligosaccharides (RFOs) are almost ubiquitous in seeds and have been hypothesized to constitute an important energy source during germination...
  56. doi Perfluorocarbon improves post-transplant survival and early kidney function following prolonged cold ischemia
    T Marada
    Department of Transplant Surgery and Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
    Eur Surg Res 44:170-8. 2010
    ..We tested whether this technique would prevent tissue damage and improve kidney function in a model of syngeneic kidney transplantation with prolonged ischemia time...
  57. ncbi Successful pancreas preservation by a perfluorocarbon-based one-layer method for subsequent pig islet isolation
    Daniel Brandhorst
    Third Medical Department, University Hospital, Rodthohl 6, 35385 Giessen, Germany
    Transplantation 79:433-7. 2005
    ..The present study examined whether PFC can be used in a one-layer method (OLM) for long-term pancreas preservation before isolation of adult pig islets...
  58. ncbi The role of raffinose in the cold acclimation response of Arabidopsis thaliana
    Ellen Zuther
    Max Planck Institut fur Molekulare Pflanzenphysiologie, 14424 Potsdam, Germany
    FEBS Lett 576:169-73. 2004
    In many plants raffinose family oligosaccharides are accumulated during cold acclimation. The contribution of raffinose accumulation to freezing tolerance is not clear...
  59. ncbi Transcript and metabolite profiling during cold acclimation of Arabidopsis reveals an intricate relationship of cold-regulated gene expression with modifications in metabolite content
    Fatma Kaplan
    Plant Molecular and Cellular Biology Program, Department of Environmental Horticulture, University of Florida, Gainesville, FL 32611, USA
    Plant J 50:967-81. 2007
    ..The present findings demonstrate that regulatory processes independent of transcript abundance represent a key part of the metabolic adjustments that occur during cold acclimation...
  60. pmc Cloning and expression analysis of a UDP-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants
    Nir Dai
    Department of Vegetable Research, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
    Plant Physiol 142:294-304. 2006
    The Cucurbitaceae translocate a significant portion of their photosynthate as raffinose and stachyose, which are galactosyl derivatives of sucrose...
  61. pmc Cloning, functional expression, and characterization of the raffinose oligosaccharide chain elongation enzyme, galactan:galactan galactosyltransferase, from common bugle leaves
    Esther M Tapernoux-Lüthi
    Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, Ch 8008 Zurich, Switzerland
    Plant Physiol 134:1377-87. 2004
    Galactan:galactan galactosyltransferase (GGT) is a unique enzyme of the raffinose family oligosaccharide (RFO) biosynthetic pathway...
  62. ncbi The preservation of liposomes by raffinose family oligosaccharides during drying is mediated by effects on fusion and lipid phase transitions
    Dirk K Hincha
    Max Planck Institut fur Molekulare Pflanzenphysiologie, D 14424 Potsdam, Germany
    Biochim Biophys Acta 1612:172-7. 2003
    b>Raffinose family oligosaccharides (RFO) have been implicated as protective agents in the cellular dehydration tolerance, especially of many plant seeds...
  63. pmc Phloem loading in Verbascum phoeniceum L. depends on the synthesis of raffinose-family oligosaccharides
    Ashlee McCaskill
    Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA
    Proc Natl Acad Sci U S A 104:19619-24. 2007
    ..It has been proposed that loading occurs symplastically in species that translocate carbohydrate primarily as raffinose family oligosaccharides (RFOs)...
  64. ncbi Transport and metabolism of raffinose family oligosaccharides in transgenic potato
    Matthew A Hannah
    Max Planck Institut fur Molekulare Pflanzenphysiologie, D 14424 Potsdam, Germany
    J Exp Bot 57:3801-11. 2006
    b>Raffinose family oligosaccharides (RFOs) are involved in the storage and transport of carbon and serve as compatible solutes for protection against abiotic stresses like drought or cold...
  65. ncbi Effect of pH on Lactobacillus fermentum growth, raffinose removal, alpha-galactosidase activity and fermentation products
    J G LeBlanc
    Centro de Referencia para Lactobacilos CERELA CONICET, Chacabuco 145, San Miguel de Tucuman, 4000 Tucuman, Argentina
    Appl Microbiol Biotechnol 65:119-23. 2004
    ..Growth was similar under all conditions assayed except at pH 4.5. These microorganisms were able to eliminate raffinose, a nondigestible alpha-oligosaccharide (NDO) found in soy products, showing a consumption rate of 0...
  66. pmc Crystal structure of inactivated Thermotoga maritima invertase in complex with the trisaccharide substrate raffinose
    François Alberto
    Architecture et Fonction des Macromolecules Biologiques, UMR6098, CNRS, Universités Aix Marseille I and II, Case 932, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
    Biochem J 395:457-62. 2006
    ..a member of the glycoside hydrolase family GH-32, readily releases beta-D-fructose from sucrose, raffinose and fructan polymers such as inulin...
  67. pmc Induction of sucrose utilization genes from Bifidobacterium lactis by sucrose and raffinose
    Marla I Trindade
    Department of Molecular and Cell Biology, University of Cape Town, Rondebosch 7701, South Africa
    Appl Environ Microbiol 69:24-32. 2003
    ..In B. lactis, total sucrase activity was induced by the presence of sucrose, raffinose, or oligofructose in the culture medium and was repressed by glucose...
  68. ncbi Suppressive effect of dietary raffinose on T-helper 2 cell-mediated immunity
    Taizo Nagura
    Department of Applied Biological Chemistry, The University of Tokyo, Bunkyo, Tokyo, Japan
    Br J Nutr 88:421-6. 2002
    The effects of the dietary oligosaccharide raffinose on immune responses, with special reference to its anti-allergic functions, were examined in vivo...
  69. ncbi O-raffinose cross-linking markedly reduces systemic and renal vasoconstrictor effects of unmodified human hemoglobin
    W Lieberthal
    Renal Division, Evans Department of Medicine, Naval Blood Research Laboratory, Boston University Medical Center, Massachusetts 02118, USA
    J Pharmacol Exp Ther 288:1278-87. 1999
    ..We compared unmodified hemoglobin with hemoglobin cross-linked with O-raffinose. Unmodified hemoglobin increased systemic vascular resistance and mean arterial pressure more than the O-..
  70. ncbi Assessment of hypolactasia and site-specific intestinal permeability by differential sugar absorption of raffinose, lactose, sucrose and mannitol
    Jan Hessels
    Clinical Laboratory, Twenteborg Hospital, Almelo, The Netherlands
    Clin Chem Lab Med 41:1056-63. 2003
    ..In this study, we evaluated the utility of raffinose, lactose, sucrose and mannitol as probe sugars and calculated their urinary recovery as a percentage of ingested ..
  71. ncbi Preservation of the liver graft with Celsior solution
    F A Garcia-Gil
    Liver Transplant Unit, Hospital Clinico Universitario Lozano Blesa, Zaragoza, Spain
    Transplant Proc 38:2385-8. 2006
    ..We studied the evolution of the liver graft with preservation in Celsior (CS) compared with University of Wisconsin solution (UW)...
  72. ncbi Current status and perspective of liver preservation solutions
    Xiao Ning Feng
    Department of Hepatobiliary Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
    Hepatobiliary Pancreat Dis Int 5:490-4. 2006
    ..In this article, we review recent advances in liver preservation solutions...
  73. ncbi The UW solution has greater potential for longer preservation periods than the Celsior solution: comparative study for ventricular and coronary endothelial function after 24-h heart preservation
    Noriyoshi Kajihara
    Department of Cardiovascular Surgery, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Eur J Cardiothorac Surg 29:784-9. 2006
    ..We compared the efficacies of the University of Wisconsin (UW) and Celsior solutions on ventricular and endothelial functions after 24-h preservation...
  74. ncbi Hypothermic machine perfusion of liver grafts for transplantation: technical development in human discard and miniature swine models
    J V Guarrera
    Department of Surgery, New York Presbyterian Hospital, New York, New York 10032, USA
    Transplant Proc 37:323-5. 2005
    ..The aim of this study was to develop a reproducible technique for liver HMP prior to initiating a clinical trial...
  75. ncbi A comparative prospective study of two available solutions for kidney and liver preservation
    Paola Pedotti
    Genetic and Molecular Epidemiology Unit IRCCS Ospedale Maggiore, Milano, Italy
    Transplantation 77:1540-5. 2004
    ..We have performed a prospective multicenter study to compare the role of these cold-storage solutions on kidney and liver recovery after transplantation...
  76. ncbi Pronlonged hypothermic machine perfusion preserves hepatocellular function but potentiates endothelial cell dysfunction in rat livers
    Hongzhi Xu
    Department of Biology, University of North Carolina, Charlotte, NC 28223, USA
    Transplantation 77:1676-82. 2004
    ..The present study was conducted to investigate endothelial and hepatocellular functions following extended HMP (> r =24 hr) in rat liver model...
  77. ncbi Comparison of histidine-tryptophan-ketoglutarate solution and University of Wisconsin solution for organ preservation in clinical pancreas transplantation
    Jonathan A Fridell
    Department of Surgery, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Transplantation 77:1304-6. 2004
    ..This study assesses the efficacy of histidine-tryptophan-ketoglutarate (HTK) compared with UW in pancreas transplantation...
  78. ncbi Impact of organ preservation using HTK for graft flush and subsequent storage in UW in rat kidney transplantation
    Volker Schmitz
    Department of Anesthesiology, University of Colorado Health Sciences Center, Denver, CO, USA
    Eur Surg Res 38:388-98. 2006
    ..Since previous reports have indicated a benefit of combining histidine-tryptophan-ketoglutarate (HTK) and University of Wisconsin (UW) solution, we evaluated the effects of initial flush with low viscosity HTK, followed by storage in UW...
  79. ncbi Comparison of histidine-tryptophan ketoglutarate solution and University of Wisconsin solution in prolonged cold preservation of kidney allografts
    Avinash Agarwal
    Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Transplantation 81:480-2. 2006
    ..This data suggests that UW and HTK have at least similar efficacy in kidney preservation at longer ischemic times...
  80. ncbi Ex-vivo study of flow dynamics and endothelial cell structure during extended hypothermic machine perfusion preservation of livers
    Shailendra Jain
    Department of Mechanical Engineering and Engineering Science, UNC Charlotte, Charlotte, NC, USA
    Cryobiology 48:322-32. 2004
    ..Histological findings confirm these results. As a result, heterogeneous flow pattern, red cell stasis, and edema occur, which may lead to the failure of these tissues following extended HMP...
  81. ncbi The influence of short-term fasting on the quality of small bowel graft preservation
    Payam Salehi
    Surgical Medical Research Institute, 1074 Dentistry Pharmacy Building, University of Alberta, Edmonton, Alta, Canada T6G 2N8
    Cryobiology 50:83-92. 2005
    ..In this study, we investigated the effect of donor nutritional status and a proven nutrient-rich preservation solution on graft quality following cold storage...
  82. ncbi Ischemic type biliary lesions in histidine-tryptophan-ketoglutarate (HTK) preserved liver grafts
    C Moench
    Department of Transplantation and Hepatobiliary Surgery, Johannes Gutenberg University, Mainz, Germany
    Int J Artif Organs 29:329-34. 2006
    ..We suggest that low viscosity of the preservation fluid by itself does not guarantee reliable perfusion of the small arteries of a liver graft and a pressure perfusion might be beneficial even in HTK solution...
  83. ncbi Kidney transplantation from non-heart-beating donors after oxygenated low-flow machine perfusion preservation with histidine-tryptophan-ketoglutarate solution
    Thomas Minor
    Surgical Research Division, University of Bonn, Sigmund Freud Strasse 25, 53127, Bonn, Germany
    Transpl Int 17:707-12. 2005
    ..In conclusion, provision of oxygen during storage is possible by low-flow perfusion with HTK as with Belzer MPS and apparently improves graft viability after transplantation...
  84. ncbi Suppression of cold ischemic injury in stored kidneys by the antimicrobial peptide bactenecin
    Jonathan F McAnulty
    Department of Surgical Sciences, University of Wisconsin, 2015 Linden Dr, Madison, WI 53706, USA
    Cryobiology 49:230-40. 2004
    ..Cold ischemic injury plays an important role in short- and long-term function of kidneys after transplant. Antimicrobial peptides have not previously been studied for their impact on cold ischemia in transplanted kidneys...
  85. ncbi Flow and pressure during liver preservation under ex situ and in situ perfusion with University of Wisconsin solution and histidine-tryptophan-ketoglutarate solution
    C Moench
    Department of Transplantation and Hepatobiliarypancreatic Surgery, Johannes Gutenberg University, Mainz, Germany
    Eur Surg Res 39:175-81. 2007
    ..No major differences between HTK and UW solutions could be detected. Therefore, an additional ex situ perfusion of the hepatic artery should be mandatory in every liver procurement...
  86. ncbi UW is superior to Celsior and HTK in the protection of human liver endothelial cells against preservation injury
    Hermann Janssen
    Klinik für Allgemein und Transplantationschirurgie, Universitatsklinikum Essen, Essen, Germany
    Liver Transpl 10:1514-23. 2004
    ..In conclusion, our cell culture model reveals the order of efficacy to protect HLEC against preservation injury as: UW >> CS > HTK...
  87. ncbi Loss of endothelium-dependent relaxation in abdominal aorta preserved in a co-storage system
    Jane M Knes
    Division of Transplantation Department of Surgery, University of Wisconsin Clinical Science Center, 600 Highland Avenue, Madison, WI 53792, USA
    Transpl Int 17:699-706. 2005
    ..We conclude that parenchymal cells exude factors during preservation by cold storage which reversibly inhibit vascular NO production. These factors could significantly impair whole organ function on reperfusion...
  88. ncbi Wash-out of the non-heart-beating donor liver: a matter of flush solution and temperature?
    Maud Bessems
    Surgical Laboratory, Department of Surgery, Academic Medical Center, AZ Amsterdam, The Netherlands
    Liver Int 26:880-8. 2006
    ..The aim of this study was to investigate the type of flush solution, temperature and anticoagulant content on the wash-out of the non-heart-beating donor (NHBD) rat liver...
  89. ncbi Histidine-tryptophan-ketoglutarate solution vs. University of Wisconsin solution for liver transplantation: a systematic review
    Li Feng
    Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, PR China
    Liver Transpl 13:1125-36. 2007
    ..Adequately powered RCTs with longer follow-up periods are required to evaluate the long-term effect of these 2 solutions...
  90. ncbi Short-term ischaemic storage of human uterine myometrium--basic studies towards uterine transplantation
    C Almén Wranning
    Department of Obstetrics and Gynecology, The Sahlgrenska Academy at Goteborg University, Sahlgrenska University Hospital, Sweden
    Hum Reprod 20:2736-44. 2005
    ..Transplantation of the uterus has been suggested as a possible future treatment of absolute uterine infertility. The tolerability of human uterine tissue to cold ischaemic storage was tested in the present study...
  91. ncbi 24-h storage of pig livers in UW, HTK, hydroxyethyl starch, and saline solution: is microdialysis an appropriate method for the continuous graft monitoring during preservation?
    Gero Puhl
    Klinik fUr Allgemein, Viszeral und Transplantationschirurgie, Charite Campus Virchow Klinikum, Humboldt Universitat, Berlin, Germany
    Transpl Int 19:303-9. 2006
    ..Whether or not glycerol monitoring could represent an additional and rational complementation to the current practice of macroscopic, microscopic and donor evaluation has to be clarified by further studies...
  92. ncbi Solutions for organ perfusion and storage: haemorheologic aspects
    P Panzera
    Department of General Surgery and Liver Transplantation, University of Bari, Bari, Italy
    Transplant Proc 37:2456-8. 2005
    ..We was also evaluated the effect of these solutions on cellular membranes by measuring acetylcholinesterase (AChE), which is a marker of RBC membrane integrity...
  93. ncbi Improved viability and reduced apoptosis in sub-zero 21-hour preservation of transplanted rat hearts using anti-freeze proteins
    Gabriel Amir
    Heart Transplantation Unit, Department of Cardiac Surgery, Sheba Medical Center, Tel Hashomer, Israel
    J Heart Lung Transplant 24:1915-29. 2005
    ..We sought to evaluate and compare the effects of prolonged sub-zero cryopreservation of transplanted rat hearts using AFP I or AFP III...
  94. ncbi Pancreas preservation with the University of Wisconsin versus Celsior solutions
    A Manrique
    Servicio de Cirugia General, Ap Digestivo y Trasplante de Organos Abdominales, Hospital Universitario Doce de Octubre, Madrid, Spain
    Transplant Proc 38:2582-4. 2006
    ..The use of Celsior solution for organ preservation has not been thoroughly studied in pancreas transplantation. The aim of this study was to compare University of Wisconsin and Celsior solutions for preservation of pancreas grafts...
  95. ncbi Pancreas preservation with University of Wisconsin and Celsior solutions: a single-center, prospective, randomized pilot study
    Ugo Boggi
    Divisione di Chirurgia Generale e Trapianti, Dipartimento di Oncologia, Trapianti e Nuove Tecnologie in Medicina, Universita di Pisa, Pisa, Italy
    Transplantation 77:1186-90. 2004
    ..We report the results of a single-center, prospective, randomized pilot study specifically designed to compare the safety profile of Celsior solution with University of Wisconsin (UW) solution in clinical pancreas transplantation...
  96. ncbi Pancreas preservation with University of Wisconsin and Celsior solutions
    U Boggi
    Divisione di Chirurgia Generale e Trapianti, Universita di Pisa, Pisa, Italy
    Transplant Proc 36:563-5. 2004
    ..Although the use of Celsior has been recently described for heart, lung, liver, and kidney transplantation, no data are available on its use for clinical pancreas preservation...
  97. doi Testing combinations of protease inhibitor and preservation solution to improve islet quality and yield
    I H Al-Abdullah
    City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    Transplant Proc 40:390-2. 2008
    ..We hypothesized that using both TLM and Pefabloc could enhance islet yield as compared with preservation in University of Wisconsin (UW) or Histidine-Tryptophan Ketoglutarate (HTK) solution...
  98. doi Organ preservation with histidine-tryptophan ketogluatarate solution in clinical pancreas transplantation: an update of the indiana university experience
    A Agarwal
    Department of Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA
    Transplant Proc 40:498-501. 2008
    ..Serum fasting blood glucose and serial amylase remained comparable at all intervals posttransplantation to those of a historical UW cohort. Within this range of cold ischemia times, HTK appears to provide effective pancreas preservation...
  99. ncbi Biochemical and morphological changes in pancreas stored under hypothermic conditions
    A Kinasiewicz
    Department of General and Transplantation Surgery, Medical University of Warsaw, 59 Nowogrodzka Street, 02 006 Warsaw, Poland
    Transplant Proc 34:655-6. 2002
  100. ncbi Evaluation of rat liver apoptotic and necrotic cell death after cold storage using UW, HTK, and Celsior
    Irene H Straatsburg
    Surgical Laboratory, Department of Surgery, Academic Medical Center, University of Amsterdam, The Netherlands
    Transplantation 74:458-64. 2002
    ..In the isolated perfused rat liver model, we compared the effects of Celsior, University of Wisconsin (UW), and histidine-tryptophan-ketoglutarate (HTK) preservation solutions on liver cell death...
  101. ncbi Pathomorphologic and microcirculatory changes and endothelin-1 expression in UW-and Celsior-preserved pancreata in experimental pancreas transplantation
    S Ludwig
    Department of Surgery II, University of Leipzig, Leipzig, Germany
    Transplant Proc 34:2364-5. 2002

Research Grants64

  1. Substrate Selection in Homologous Bacterial Transporters
    Manuel Varela; Fiscal Year: 2004
    ..coli (LacY) also confer sugar selection in homologous transporters for melibiose (MelY), raffinose (RafB) and sucrose (CseB)...
  2. Heptocellular Response to Cryopreservation
    Mehmet Toner; Fiscal Year: 2006
    ..The proposed studies will be carried out using several key sugars including trehalose, sucrose, raffinose, stachyose, and mixtures thereof...
  3. Model of Transport in Renal Medullary Microvasculature
    AURELIE EDWARDS; Fiscal Year: 2007
    ..Permeability of DVR to sodium and raffinose varies with perfusion rate; this dependence may be mediated by NO generation...
  4. VASOMOTOR CONTROL OF DESCENDING VASA RECTA
    THOMAS PALLONE; Fiscal Year: 2009
    ..We will determine the role of VOCC and nonselective cation channels in Angll signaling and test a role for protein kinase C in their activation. ..
  5. Factors affecting ice formation in cells and their relevance to cryopreservation
    Peter Mazur; Fiscal Year: 2009
    ....
  6. MECHANISMS OF MYOCARDIAL ISCHEMIC INJURY IN DIABETES
    Judy Kersten; Fiscal Year: 2007
    ....
  7. MECHANISMS OF MYOCARDIAL ISCHEMIC INJURY IN DIABETES
    Judy R Kersten; Fiscal Year: 2010
    ..abstract_text> ..
  8. Genetic Dissection of a Motor Central Pattern Generator
    JOHN BOUGHTER; Fiscal Year: 2007
    ..Comparison of the genes and mechanisms underlying stereotyped repetitive movements versus plastic behaviors will be a source of information relevant for a number of human CMS disorders involving abnormal repetitive movements. ..
  9. The Transporter Classification Database (TCDB)
    Milton Saier; Fiscal Year: 2007
    ..6) Develop approaches that will allow integration of the different lines of functional information revealed in specific aims #4 and 5. ..
  10. The Transporter Classification Database (TCDB)
    Milton Saier; Fiscal Year: 2009
    ..6) Develop approaches that will allow integration of the different lines of functional information revealed in specific aims #4 and 5. ..
  11. Factors affecting ice formation in cells and their relevance to cryopreservation
    Peter Mazur; Fiscal Year: 2007
    ..The final goal will be to use the mechanistic data on IIF to devise better methods of avoiding it and thus improve success in the cryopreservation of cells that currently can not be well cryopreserved. ..
  12. Factors affecting ice formation in cells and their relevance to cryopreservation
    Peter Mazur; Fiscal Year: 2009
    ..The final goal will be to use the mechanistic data on IIF to devise better methods of avoiding it and thus improve success in the cryopreservation of cells that currently can not be well cryopreserved. ..
  13. SENSORY CODING IN TASTE
    JOHN BOUGHTER; Fiscal Year: 2007
    ..These experiments test the hypothesis that there are differences in voltage- activated conductances and cell morphology between neurons projecting to the VPMpc and the CeA. ..
  14. Sugars as Novel Cryoprotectants for Primate Oocytes
    Ali Eroglu; Fiscal Year: 2009
    ..We predict that the proposed research will result in a reliable preservation technique for primate oocytes by circumventing many of the difficulties associated with conventional protocols. ..
  15. AQUAPORINS, ICE FORMATION IN CELLS,/CRYOPRESERVATION
    Peter Mazur; Fiscal Year: 2006
    ..abstract_text> ..
  16. CYROBIOLOGICAL PRESERVATION OF ANOPHELES EMBRYOS
    Peter Mazur; Fiscal Year: 2001
    ..Such a mutant has been isolated in a related species, Anopheles quadrimaculatus. We also propose to collaborate with the Laboratory of Parasitic Diseases at NIH in the cryopreservation of A gambiae larvae. ..
  17. ATP DEPENDENT PROTEIN KINASES IN GRAM POSITIVE BACTERIA
    Milton Saier; Fiscal Year: 2000
    ..The knowledge gained should be applicable to the control of pathogenic bacteria. The protein kinase-mediated regulatory processes under study are expected to be relevant to analogous processes in eukaryotic organisms. ..
  18. MICROVASCULAR TRANSPORT IN THE RENAL MEDULLA
    THOMAS PALLONE; Fiscal Year: 2002
    ..Exchange of albumin and middle molecular weight solutes will also be simulated. ..
  19. MECHANISMS OF MYOCARDIAL ISCHEMIC INJURY IN DIABETES
    Judy Kersten; Fiscal Year: 2004
    ..Thus, this proposal will delineate mechanisms responsible for the adverse consequences of diabetes and hyperglycemia during myocardial ischemia using an integrated approach of in vivo and cellular techniques. ..
  20. Calcium Signaling in Vasa Recta Endothelium
    THOMAS PALLONE; Fiscal Year: 2004
    ..We will test whether DVR contractions to norepinephrine, Angll and KCI are increased, measure NO production and assess effects of OH on endothelial and pericyte Ca2+ signaling. ..
  21. ATP-DEPENDENT PROTEIN KINASES IN GRAM-POSITIVE BACTERIA
    Milton Saier; Fiscal Year: 2004
    ..abstract_text> ..
  22. Bitter Taste: Physiology and Behavior
    JOHN BOUGHTER; Fiscal Year: 2004
    ..By examining these strain differences in receptor cell function, these studies should help to link ongoing molecular advances to the neural mechanisms underlying bitter taste function. ..
  23. VASOMOTOR CONTROL OF DESCENDING VASA RECTA
    THOMAS PALLONE; Fiscal Year: 2003
    ....
  24. Role of SLc11a1 Gen in Warm Ischemia-Reperfusion Injury
    Samuel Wyllie; Fiscal Year: 2006
    ..These studies will identify molecular mechanisms by which Slc11a1 modulates liver I/R injury, and will provide a novel target for therapeutic intervention in I/R-mediated organ injury. ..