Summary: A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.

Top Publications

  1. pmc Spatially restricted patterning cues provided by heparin-binding VEGF-A control blood vessel branching morphogenesis
    Christiana Ruhrberg
    Endothelial Cell Biology Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, United Kingdom
    Genes Dev 16:2684-98. 2002
  2. ncbi Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate
    Y Chen
    Department of Internal Medicine, University of Michigan, Ann Arbor 48109, USA
    Nat Med 3:866-71. 1997
  3. ncbi A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF
    S Higashiyama
    Department of Surgical Research, Children s Hospital, Boston, MA 02115
    Science 251:936-9. 1991
  4. ncbi Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells
    N Ferrara
    Department of Pharmacological Sciences, Genentech Inc, South San Francisco, CA 94080
    Biochem Biophys Res Commun 161:851-8. 1989
  5. ncbi Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine cilia
    Ania T Deutscher
    Industry and Investment NSW, Elizabeth Macarthur Agricultural Institute, NSW, Australia
    Mol Microbiol 78:444-58. 2010
  6. ncbi P159 is a proteolytically processed, surface adhesin of Mycoplasma hyopneumoniae: defined domains of P159 bind heparin and promote adherence to eukaryote cells
    Tracey A Burnett
    NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Camden, NSW 2570, Australia
    Mol Microbiol 60:669-86. 2006
  7. pmc Human papillomavirus infection requires cell surface heparan sulfate
    T Giroglou
    Institute for Medical Microbiology and Hygiene, University of Mainz, 55101 Mainz, Germany
    J Virol 75:1565-70. 2001
  8. pmc Structure of a Plasmodium falciparum PfEMP1 rosetting domain reveals a role for the N-terminal segment in heparin-mediated rosette inhibition
    Alexandre Juillerat
    Institut Pasteur, Unité d Immunologie Structurale, 75015 Paris, France
    Proc Natl Acad Sci U S A 108:5243-8. 2011
  9. ncbi Improving the cardio protective effect of aFGF in ischemic myocardium with ultrasound-mediated cavitation of heparin modified microbubbles: preliminary experiment
    Ying Zheng Zhao
    Wenzhou Medical College, Wenzhou City, Zhejiang Province, China
    J Drug Target 20:623-31. 2012
  10. pmc Alboserpin, a factor Xa inhibitor from the mosquito vector of yellow fever, binds heparin and membrane phospholipids and exhibits antithrombotic activity
    Eric Calvo
    Section of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
    J Biol Chem 286:27998-8010. 2011

Research Grants

Detail Information

Publications317 found, 100 shown here

  1. pmc Spatially restricted patterning cues provided by heparin-binding VEGF-A control blood vessel branching morphogenesis
    Christiana Ruhrberg
    Endothelial Cell Biology Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, United Kingdom
    Genes Dev 16:2684-98. 2002
    ..We found that VEGF(120/120) mouse embryos, engineered to express solely an isoform of VEGF-A that lacks heparin-binding, and therefore extracellular matrix interaction domains, exhibited a specific decrease in capillary ..
  2. ncbi Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate
    Y Chen
    Department of Internal Medicine, University of Michigan, Ann Arbor 48109, USA
    Nat Med 3:866-71. 1997
    ..b>Heparin, highly sulfated heparan sulfate, and the polysulfonate pharmaceutical Suramin effectively prevented dengue virus ..
  3. ncbi A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF
    S Higashiyama
    Department of Surgical Research, Children s Hospital, Boston, MA 02115
    Science 251:936-9. 1991
    Macrophage-like U-937 cells secrete a 22-kilodalton heparin-binding growth factor that is mitogenic for BALB-3T3 fibroblasts and smooth muscle cells, but not endothelial cells...
  4. ncbi Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells
    N Ferrara
    Department of Pharmacological Sciences, Genentech Inc, South San Francisco, CA 94080
    Biochem Biophys Res Commun 161:851-8. 1989
    ..bovine pituitary follicular cells and purified to homogeneity by a combination of ammonium sulfate precipitation, heparin-sepharose affinity chromatography and two reversed phase HPLC steps...
  5. ncbi Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine cilia
    Ania T Deutscher
    Industry and Investment NSW, Elizabeth Macarthur Agricultural Institute, NSW, Australia
    Mol Microbiol 78:444-58. 2010
    ..Eight pentapeptide repeats in R1 are sufficient to bind porcine cilia; however, both R1 and R2 are needed to bind heparin. Mhp271, a paralogue of P97, is the only other M. hyopneumoniae protein to contain both R1 and R2 repeats...
  6. ncbi P159 is a proteolytically processed, surface adhesin of Mycoplasma hyopneumoniae: defined domains of P159 bind heparin and promote adherence to eukaryote cells
    Tracey A Burnett
    NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Camden, NSW 2570, Australia
    Mol Microbiol 60:669-86. 2006
    ..b>Heparin is known to inhibit adherence of M. hyopneumoniae to porcine respiratory epithelial cilia. M...
  7. pmc Human papillomavirus infection requires cell surface heparan sulfate
    T Giroglou
    Institute for Medical Microbiology and Hygiene, University of Mainz, 55101 Mainz, Germany
    J Virol 75:1565-70. 2001
    ..Pseudoinfection was inhibited by heparin but not dermatan or chondroitin sulfate, reduced by reducing the level of surface sulfation, and abolished by ..
  8. pmc Structure of a Plasmodium falciparum PfEMP1 rosetting domain reveals a role for the N-terminal segment in heparin-mediated rosette inhibition
    Alexandre Juillerat
    Institut Pasteur, Unité d Immunologie Structurale, 75015 Paris, France
    Proc Natl Acad Sci U S A 108:5243-8. 2011
    ..b>Heparin and other sulfated oligosaccharides, however, can disrupt rosettes, suggesting that therapeutic approaches to ..
  9. ncbi Improving the cardio protective effect of aFGF in ischemic myocardium with ultrasound-mediated cavitation of heparin modified microbubbles: preliminary experiment
    Ying Zheng Zhao
    Wenzhou Medical College, Wenzhou City, Zhejiang Province, China
    J Drug Target 20:623-31. 2012
    ..factor (aFGF) intravenous delivery to the ischemic myocardium of rats by ultrasonic microbubbles modified with heparin. Heparin modified microbubbles (HMB) were prepared by the freeze-dried method...
  10. pmc Alboserpin, a factor Xa inhibitor from the mosquito vector of yellow fever, binds heparin and membrane phospholipids and exhibits antithrombotic activity
    Eric Calvo
    Section of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
    J Biol Chem 286:27998-8010. 2011
    ..Alboserpin displays high affinity binding to heparin (K(D) ~ 20 nM), but no change in FXa inhibition was observed in the presence of the cofactor, implying that ..
  11. pmc Treatment and prevention of heparin-induced thrombocytopenia: Antithrombotic Therapy and Prevention of Thrombosis, 9th ed: American College of Chest Physicians Evidence-Based Clinical Practice Guidelines
    Lori Ann Linkins
    Department of Medicine, McMaster University, Juravinski Hospital, Rm M0118, 1280 Main St W, Hamilton, ON, L8S 4K1, Canada
    Chest 141:e495S-530S. 2012
    b>Heparin-induced thrombocytopenia (HIT) is an antibody-mediated adverse drug reaction that can lead to devastating thromboembolic complications, including pulmonary embolism, ischemic limb necrosis necessitating limb amputation, acute ..
  12. pmc Critical role of heparin binding domains of ameloblastin for dental epithelium cell adhesion and ameloblastoma proliferation
    Akira Sonoda
    Section of Pediatric Dentistry, Division of Oral Health, Growth and Development, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 284:27176-84. 2009
    ..By using recombinant AMBN proteins, we found that AMBN had heparin binding domains at the C-terminal half and that these domains were critical for AMBN binding to dental epithelial ..
  13. ncbi Mhp493 (P216) is a proteolytically processed, cilium and heparin binding protein of Mycoplasma hyopneumoniae
    Jody Wilton
    NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Camden, NSW 2570, Australia
    Mol Microbiol 71:566-82. 2009
    ..b>Heparin binds to M. hyopneumoniae cells in a dose-dependent manner and blocks its ability to adhere to porcine cilia...
  14. ncbi Mast cells increase vascular permeability by heparin-initiated bradykinin formation in vivo
    Chris Oschatz
    Clinical Chemistry, Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden
    Immunity 34:258-68. 2011
    ..We report a paracrine mechanism by which mast cell-released heparin increases vascular permeability in vivo...
  15. pmc Single amino acid changes can influence titer, heparin binding, and tissue tropism in different adeno-associated virus serotypes
    Zhijian Wu
    Gene Therapy Center, 7113 Thurston Building, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7352, USA
    J Virol 80:11393-7. 2006
    ..acid residues that differ between AAV1 and AAV6 revealed that a lysine-to-glutamate change (K531E) suppresses the heparin binding ability of AAV6...
  16. pmc Two domains within the Mycoplasma hyopneumoniae cilium adhesin bind heparin
    Cheryl Jenkins
    Microbiology and Immunology, Elizabeth Macarthur Agricultural Institute, PMB 8, Camden, New South Wales 2570, Australia
    Infect Immun 74:481-7. 2006
    ..hyopneumoniae is lacking. However, heparin and other sulfated polysaccharides are known to block the binding of M...
  17. ncbi Specific molecular interactions of oversulfated chondroitin sulfate E with various heparin-binding growth factors. Implications as a physiological binding partner in the brain and other tissues
    Sarama Sathyaseelan Deepa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Japan
    J Biol Chem 277:43707-16. 2002
    We previously observed that the cortical neuronal cell adhesion mediated by midkine (MK), a heparin (Hep)-binding growth factor, is specifically inhibited by oversulfated chondroitin sulfate-E (CS-E) (Ueoka, C., Kaneda, N., Okazaki, I...
  18. ncbi FGFs, heparan sulfate and FGFRs: complex interactions essential for development
    D M Ornitz
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Bioessays 22:108-12. 2000
    ..All FGFs have a high affinity for the glycosaminoglycan heparin and for cell surface heparan sulfate proteoglycans...
  19. ncbi Antibody-based assay for N-deacetylase activity of heparan sulfate/heparin N-deacetylase/N-sulfotransferase (NDST): novel characteristics of NDST-1 and -2
    Jacob van den Born
    Division of Nephrology, University Hospital St Radboud, PO Box 9101, 6500 HB, Nijmegen, The Netherlands
    Glycobiology 13:1-10. 2003
    ..N-deacetylase/N-sulfotransferases (NDSTs), which are key enzymes in sulfation of heparan sulfate (HS)/heparin. The assay is based on the recognition of NDST-generated N-unsubstituted glucosamine units in Escherichia coli K5 ..
  20. ncbi Caring for the critically ill patient. High-dose antithrombin III in severe sepsis: a randomized controlled trial
    B L Warren
    Brown University School of Medicine, Infectious Disease Division, Memorial Hospital of Rhode Island, 111 Brewster St, Pawtucket, RI 02860, USA
    JAMA 286:1869-78. 2001
    ..In the subgroup of patients who did not receive concomitant heparin during the 4-day treatment phase (n = 698), the 28-day mortality was nonsignificantly lower in the antithrombin ..
  21. pmc Passage of dengue virus type 4 vaccine candidates in fetal rhesus lung cells selects heparin-sensitive variants that result in loss of infectivity and immunogenicity in rhesus macaques
    Germán Añez
    Molecular Viral Biology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 8005, USA
    J Virol 83:10384-94. 2009
    ..in FRhL cells showed increased affinity for heparan sulfate compared to the unpassaged DENV-4, as measured by heparin binding and infectivity inhibition assays...
  22. pmc Heparin impairs angiogenesis through inhibition of microRNA-10b
    Xiaokun Shen
    Glycochemistry and Glycobiology Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai
    J Biol Chem 286:26616-27. 2011
    b>Heparin, which has been used as an anticoagulant drug for decades, inhibits angiogenesis, whereas thrombin promotes tumor-associated angiogenesis...
  23. ncbi Heparin increases the infectivity of Human Papillomavirus type 16 independent of cell surface proteoglycans and induces L1 epitope exposure
    Carla Cerqueira
    Emmy Noether Group Virus Endocytosis, Institutes of Molecular Virology and Medical Biochemistry, University of Munster, Munster, Germany
    Cell Microbiol 15:1818-36. 2013
    ..Here, we analyse the phenomenon that preincubation of HPV-16 with increasing concentrations of heparin results in partial restoration rather than more efficient inhibition of infection...
  24. pmc Unfractionated heparin and new heparin analogues from ascidians (chordate-tunicate) ameliorate colitis in rats
    Celso L R Belmiro
    Laboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho HUCFF and Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro UFRJ, Rio de Janeiro 21941 913, Brazil
    J Biol Chem 284:11267-78. 2009
    The anti-inflammatory effect of mammalian heparin analogues, named dermatan sulfate and heparin, isolated from the ascidian Styela plicata was accessed in a TNBS-induced colitis model in rats...
  25. pmc N-arginine dibasic convertase is a specific receptor for heparin-binding EGF-like growth factor that mediates cell migration
    E Nishi
    Department of Surgical Research, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    EMBO J 20:3342-50. 2001
    b>Heparin-binding epidermal growth factor-like growth factor (HB-EGF), a mitogen and chemotactic factor, binds to two receptor tyrosine kinases, erbB1 and erbB4...
  26. ncbi Regional citrate versus heparin anticoagulation for continuous renal replacement therapy: a meta-analysis of randomized controlled trials
    Mei Yi Wu
    Division of Nephrology, Department of Internal Medicine, Taipei Medical University Shuang Ho Hospital, Taipei, Taiwan
    Am J Kidney Dis 59:810-8. 2012
    Anticoagulation of the extracorporeal circuit is required in continuous renal replacement therapy (CRRT). Heparin is the classic choice for anticoagulation, although it may increase the risk of bleeding...
  27. ncbi Heparin enhances the cell-protein misfolding cyclic amplification efficiency of variant Creutzfeldt-Jakob disease
    Takashi Yokoyama
    Research and Development Division, Benesis Corporation, c o Division of Neurological Science, Department of Prion Research, Tohoku University Graduate School of Medicine, 2 1 Seiryo machi, Aoba ku, Sendai, Japan
    Neurosci Lett 498:119-23. 2011
    ..PrP(res) amounts after cell-PMCA were analyzed by western blotting. Heparin, dermatan sulfate, and dextran sulfate (average molecular weight [MW] 1400kDa) enhanced efficiency, but dextran ..
  28. pmc Characterization of heparin-binding site of tissue transglutaminase: its importance in cell surface targeting, matrix deposition, and cell signaling
    Zhuo Wang
    School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, United Kingdom
    J Biol Chem 287:13063-83. 2012
    ..A novel regulatory mechanism for TG2 translocation into the extracellular compartment that depends upon TG2 conformation and the binding of HS is proposed...
  29. pmc NMR structural determinants of eosinophil cationic protein binding to membrane and heparin mimetics
    María Flor García-Mayoral
    Instituto de Quimica Fisica Rocasolano, CSIC, Madrid, Spain
    Biophys J 98:2702-11. 2010
    ..We have used NMR spectroscopy to characterize the binding of ECP to membrane and heparin mimetics at a residue level...
  30. ncbi Heparan sulfate/heparin oligosaccharides protect stromal cell-derived factor-1 (SDF-1)/CXCL12 against proteolysis induced by CD26/dipeptidyl peptidase IV
    Rabia Sadir
    Institut de Biologie Structurale, CNRS Commissariat à l Enérgie Atomique Université Joseph Fourier, Unité Mixte de Recherche 5075, 41 Rue Horowitz, 38027 Grenoble Cedex 01, France
    J Biol Chem 279:43854-60. 2004
    ..We report here that heparin and HS specifically prevent the processing of SDF-1 by DPP IV expressed by Caco-2 cells...
  31. pmc Self-assembling nanocomplexes by combining ferumoxytol, heparin and protamine for cell tracking by magnetic resonance imaging
    Mya S Thu
    Frank Laboratory and Laboratory of Diagnostic Radiology Research, Department of Radiology and Imaging Sciences, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Med 18:463-7. 2012
    ..cell-labeling approach that combines three US Food and Drug Administration (FDA)-approved drugs--ferumoxytol, heparin and protamine--in serum-free medium to form self-assembling nanocomplexes that effectively label cells for in ..
  32. ncbi The potential role of heparin in assisted conception
    Scott M Nelson
    Reproductive and Maternal Medicine, Division of Developmental Medicine, University of Glasgow, Glasgow Royal Infirmary, 10 Alexandra Parade, Glasgow G31 ER, UK
    Hum Reprod Update 14:623-45. 2008
    Heparan sulphates play key roles in conception and early pregnancy events. The role of heparin, a structural analogue, and its application to assisted conception, is largely unknown.
  33. pmc A systems biology approach for the investigation of the heparin/heparan sulfate interactome
    Alessandro Ori
    Institute of Integrative Biology and Centre for Glycobiology, University of Liverpool, Liverpool, United Kingdom
    J Biol Chem 286:19892-904. 2011
    ..We collected 435 human proteins interacting with HS or the structurally related heparin by integrating literature-derived and affinity proteomics data...
  34. pmc Characterization of the chemokine CXCL11-heparin interaction suggests two different affinities for glycosaminoglycans
    India C Severin
    Merck Serono Geneva Research Centre, 9 Chemin des Mines, 1202 Geneva, Switzerland
    J Biol Chem 285:17713-24. 2010
    ..residues in the C-terminal helix (the 50s cluster, (57)KSKQAR(62)) along with Lys(17), significantly impaired heparin binding in vitro, identifying these residues as components of the dominant epitope...
  35. ncbi The Mycoplasma gallisepticum OsmC-like protein MG1142 resides on the cell surface and binds heparin
    Cheryl Jenkins
    Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Camden, NSW 2570, Australia
    Microbiology 153:1455-63. 2007
    ..In this study, M. gallisepticum whole cells were shown to bind heparin in a specific and saturable manner. Heparin also significantly inhibited the binding of M...
  36. ncbi Antithrombin-mediated anticoagulant activity of sulfated polysaccharides: different mechanisms for heparin and sulfated galactans
    Fabio R Melo
    Laboratório de Tecido Conjuntivo, Hospital Universitario Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Caixa Postal 68041, Rio de Janeiro, RJ 21941 590, Brazil
    J Biol Chem 279:20824-35. 2004
    ..3) Sulfated galactans require significantly longer chains than heparin to achieve anticoagulant activity...
  37. ncbi Stability of PEI-DNA and DOTAP-DNA complexes: effect of alkaline pH, heparin and serum
    I Moret
    , Facultad de Medicina, Universitat de Valencia, , Valencia, Spain
    J Control Release 76:169-81. 2001
    ..3 to 13.4) or by adding heparin. The stability against degradation was tested in vitro, by incubating the complexes at 37 degrees C in the ..
  38. ncbi Heparin-binding epidermal growth factor-like growth factor is an autocrine growth factor for human keratinocytes
    K Hashimoto
    Department of Dermatology, Osaka University Medical School, Japan
    J Biol Chem 269:20060-6. 1994
    Since heparin-binding epidermal growth factor (HB-EGF) is a member of the EGF family and binds to EGF receptor, we tested recombinant HB-EGF for its ability to stimulate human keratinocyte proliferation...
  39. ncbi Iduronic acid-containing glycosaminoglycans on target cells are required for efficient respiratory syncytial virus infection
    L K Hallak
    Immunology Microbiology, Biochemistry and Pathology, Rush Presbyterian St Luke s Medical Center, Chicago, IL 60612, USA
    Virology 271:264-75. 2000
    ..experiments in which RSV was preincubated with various soluble GAGs revealed the relative blocking order of: heparin > heparan sulfate > chondroitin sulfate B. Iduronic acid is a component common to these GAGs...
  40. pmc Heparin-binding-hemagglutinin-induced IFN-gamma release as a diagnostic tool for latent tuberculosis
    Jean Michel Hougardy
    Laboratory of Vaccinology and Mucosal Immunity, Hopital Erasme, Universite Libre de Bruxelles, Brussels, Belgium
    PLoS ONE 2:e926. 2007
    ..We have therefore evaluated the potential of the novel antigen heparin-binding hemagglutinin (HBHA) for in vitro detection of LTBI.
  41. pmc Heparin stimulates Staphylococcus aureus biofilm formation
    Robert M Q Shanks
    Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Infect Immun 73:4596-606. 2005
    b>Heparin, known for its anticoagulant activity, is commonly used in catheter locks...
  42. ncbi Induction and expression of heparin-binding EGF-like growth factor in human pancreatic cancer
    M S Kobrin
    Department of Medicine, University of California, Irvine 92717
    Biochem Biophys Res Commun 202:1705-9. 1994
    b>Heparin-binding EGF-like growth factor (HB-EGF) is a polypeptide with an apparent molecular weight of 22 kilodalton that is related to epidermal growth factor (EGF) and that binds and activates the EGF receptor...
  43. ncbi Regulation of Wnt signaling and embryo patterning by an extracellular sulfatase
    G K Dhoot
    Department of Basic Veterinary Sciences, The Royal Veterinary College, University of London, Royal College Street, London NW1 OTU, UK
    Science 293:1663-6. 2001
  44. pmc Mutations in factor H reduce binding affinity to C3b and heparin and surface attachment to endothelial cells in hemolytic uremic syndrome
    Tamara Manuelian
    Hans Knoell Institute for Natural Products Research, Beutenbergstrasse 11a, D 07745 Jena, Germany
    J Clin Invest 111:1181-90. 2003
    ..All three mutations cause reduced binding to the central complement component C3b/C3d to heparin, as well as to endothelial cells...
  45. ncbi Regional citrate versus systemic heparin anticoagulation for continuous renal replacement in critically ill patients
    Demetrios J Kutsogiannis
    Division of Critical Care Medicine, Department of Public Health Sciences, The University of Alberta, Edmonton, Alberta, Canada
    Kidney Int 67:2361-7. 2005
    We determined the effect of regional citrate versus systemic heparin anticoagulation for continuous renal replacement therapy in critically ill subjects suffering from acute renal failure who were not at high risk for hemorrhagic ..
  46. ncbi Heparin plus a glycoprotein IIb/IIIa inhibitor versus bivalirudin monotherapy and paclitaxel-eluting stents versus bare-metal stents in acute myocardial infarction (HORIZONS-AMI): final 3-year results from a multicentre, randomised controlled trial
    Gregg W Stone
    New York Presbyterian Hospital, Columbia University Medical Center, New York, NY 10022, USA
    Lancet 377:2193-204. 2011
    ..Primary results of the HORIZONS-AMI trial have been previously reported. In this final report, we aimed to assess 3-year outcomes...
  47. pmc Tissue culture adaptation of foot-and-mouth disease virus selects viruses that bind to heparin and are attenuated in cattle
    D Sa-Carvalho
    Plum Island Animal Disease Center, Agricultural Research Service, North Atlantic Area, U S Department of Agriculture, Greenport, New York 11944, USA
    J Virol 71:5115-23. 1997
    ..viruses demonstrated that positively charged residues are required at both 2134 and 3056 for binding to heparin. Taken together, these results indicate that in vitro cultivation of FMDV type O selects viruses that bind to ..
  48. pmc Sulfated K5 Escherichia coli polysaccharide derivatives as wide-range inhibitors of genital types of human papillomavirus
    David Lembo
    Department of Clinical and Biological Sciences, University of Turin, San Luigi Gonzaga Hospital, Regione Gonzole 10, 10043 Orbassano, Turin, Italy
    Antimicrob Agents Chemother 52:1374-81. 2008
    ..and biological assays to prepare a collection of sulfated K5 derivatives with a backbone structure resembling the heparin/heparan biosynthetic precursor and to test them for their anti-HPV activity...
  49. ncbi The heparin-binding domain confers diverse functions of VEGF-A in development and disease: a structure-function study
    Dominik Krilleke
    Ocular Biology and Therapeutics, UCL Institute of Ophthalmology, 11 43 Bath Street, London EC1V 9EL, UK
    Biochem Soc Trans 37:1201-6. 2009
    ..isoforms of VEGF (vascular endothelial growth factor)-A, including VEGF(164(165)), contain a highly basic HBD (heparin-binding domain)...
  50. ncbi Antithrombin, heparin, and heparan sulfate
    Steven M Opal
    Infectious Disease Division, Brown Medical School, Providence, RI 02860, USA
    Crit Care Med 30:S325-31. 2002
    ..The interaction between antithrombin and specific polysulfated, acidic oligosaccharide moieties found on heparin and related proteoglycan molecules within the circulation and on endothelial surfaces will also be examined.
  51. ncbi Heparin-bonded covered stents versus bare-metal stents for complex femoropopliteal artery lesions: the randomized VIASTAR trial (Viabahn endoprosthesis with PROPATEN bioactive surface [VIA] versus bare nitinol stent in the treatment of long lesions in sup
    Johannes Lammer
    Departments of Cardiovascular and Interventional Radiology, Medical University Vienna, Vienna, Austria Electronic address
    J Am Coll Cardiol 62:1320-7. 2013
    ..The hypothesis that endovascular treatment with covered stents has equal risks but higher efficacy than bare-metal stents (BMS) in long femoropopliteal artery disease was tested...
  52. ncbi Evaluation of the Becton-Dickinson rapid serum tube: does it provide a suitable alternative to lithium heparin plasma tubes?
    Goce Dimeski
    School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia
    Clin Chem Lab Med 48:651-7. 2010
    ..Although lithium heparin plasma has unique problems, it has become an alternative in hospital-based laboratories.
  53. ncbi Cell type-specific differences in glycosaminoglycans modulate the biological activity of a heparin-binding peptide (RKRLQVQLSIRT) from the G domain of the laminin alpha1 chain
    M P Hoffman
    Craniofacial Developmental Biology and Regeneration Branch, NIDCR, National Institutes of Health, Bethesda, Maryland 20892 4370, USA
    J Biol Chem 276:22077-85. 2001
    ..cell line (B16F10) both bind to the peptides, but cell attachment was inhibited by glycosaminoglycans, modified heparin, and sized heparin fragments in a cell type-specific manner...
  54. ncbi Heparin-coated superparamagnetic iron oxide for in vivo MR imaging of human MSCs
    Ji Hye Lee
    Department of Bioengineering, Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul 133 791, Republic of Korea
    Biomaterials 33:4861-71. 2012
    ..Here, we present superparamagnetic iron oxide (SPIO) nanoparticles coated with unfractionated heparin (UFH-SPIO) as a new negative contrast agent for in vivo MR imaging of hMSCs...
  55. ncbi His-384 allotypic variant of factor H associated with age-related macular degeneration has different heparin binding properties from the non-disease-associated form
    Simon J Clark
    Medical Research Council MRC Immunochemistry Unit and Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, United Kingdom
    J Biol Chem 281:24713-20. 2006
    ..Here, using a recombinant construct, we show that amino acid 384 is adjacent to a heparin-binding site in CCP7 of factor H and demonstrate that the allotypic variants differentially recognize heparin...
  56. ncbi Effects of citrate acid concentrate (citrasate®) on heparin N requirements and hemodialysis adequacy: a multicenter, prospective noninferiority trial
    Jeffrey J Sands
    Fresenius Medical Care North America, Celebration, FL 34747, USA
    Blood Purif 33:199-204. 2012
    ..Previous studies suggest CD may improve dialysis adequacy and decrease heparin requirements, presumably due to nonsystemic anticoagulant effects in the dialyzer.
  57. ncbi Heparin-induced thrombocytopenia: in vitro studies on the interaction of dabigatran, rivaroxaban, and low-sulfated heparin, with platelet factor 4 and anti-PF4/heparin antibodies
    Krystin Krauel
    Institut für Immunologie und Transfusionsmedizin, Universitä Greifswald, Sauerbruchstrasse, Greifswald, Germany
    Blood 119:1248-55. 2012
    b>Heparin is a widely used anticoagulant. Because of its negative charge, it forms complexes with positively charged platelet factor 4 (PF4). This can induce anti-PF4/heparin IgG Abs...
  58. ncbi Synthesis of heparin oligosaccharides and their interaction with eosinophil-derived neurotoxin
    Shang Cheng Hung
    Genomics Research Center, Academia Sinica, Taipei, Taiwan
    Org Biomol Chem 10:760-72. 2012
    A convenient route for the synthesis of heparin oligosaccharides involving regioselective protection of D-glucosamine and a concise preparation of rare L-ido sugars from diacetone α-D-glucose is described...
  59. ncbi Effect of heparin on neuroprotection against spinal cord ischemia and reperfusion in rats
    K Korkmaz
    Department of Cardiovascular Surgery, Ankara Numune Educational and Research Hospital, Turkey
    Eur Rev Med Pharmacol Sci 17:522-30. 2013
    ..We investigated the effect of the degree of anticoagulation on spinal cord I/R injury and whether heparin is protective against I/R injury beside its anticoagulant properties.
  60. ncbi Induction of heparin-binding EGF-like growth factor expression during myogenesis. Activation of the gene by MyoD and localization of the transmembrane form of the protein on the myotube surface
    X Chen
    Department of Surgery, Children s Hospital, Boston, Massachusetts 02115, USA
    J Biol Chem 270:18285-94. 1995
    b>Heparin-binding epidermal growth factor-like growth factor (HB-EGF) gene expression and protein localization were analyzed during the process of myogenic differentiation. The mouse HB-EGF gene was isolated, and a 1...
  61. ncbi Early intravenous unfractionated heparin and mortality in septic shock
    Ryan Zarychanski
    Section of Critical Care Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
    Crit Care Med 36:2973-9. 2008
    ..Unfractionated heparin is a known anticoagulant, which also possesses anti-inflammatory properties...
  62. ncbi Comparison of efficacy of silicone gel, silicone gel sheeting, and topical onion extract including heparin and allantoin for the treatment of postburn hypertrophic scars
    Huseyin Karagoz
    Maresal Cakmak Military Hospital, Department of Plastic and Reconstructive Surgery, Erzurum, Turkey
    Burns 35:1097-103. 2009
    ..the efficacy of silicone gel (Scarfade), silicone gel sheet (Epi-Derm), and topical onion extract including heparin and allantoin (Contractubex) for the treatment of hypertrophic scars...
  63. pmc Hyaluronic acid-based hydrogels functionalized with heparin that support controlled release of bioactive BMP-2
    Gajadhar Bhakta
    Institute of Medical Biology, A STAR, 8A Biomedical Grove, 06 06 Immunos, Singapore 138648, Singapore
    Biomaterials 33:6113-22. 2012
    ..b>Heparin (HP), a highly sulfated glycosaminoglycan (GAG) that avidly binds BMP-2, has inherent biological properties that ..
  64. ncbi Rapid induction of heparin-binding epidermal growth factor/diphtheria toxin receptor expression by Raf and Ras oncogenes
    S A McCarthy
    DNAX Research Institute, Palo Alto, California 94304, USA
    Genes Dev 9:1953-64. 1995
    ..Through this approach the gene encoding heparin-binding epidermal growth factor (HB-EGF) was identified as an immediate-early transcriptional target of oncogenic ..
  65. ncbi Impact of intravenous heparin on quantification of circulating microRNAs in patients with coronary artery disease
    D Kaudewitz
    Prof Manuel Mayr, King s British Heart Foundation Centre, King s College London, London, SE5 9NU, UK, Tel 44 02078 485132, Fax 44 2078485296, E mail
    Thromb Haemost 110:609-15. 2013
    ..Circulating levels of microRNAs have been measured in various studies related to cardiovascular disease. Heparin is a potential confounder of microRNA measurements due to its known interference with polymerase chain reactions...
  66. ncbi Regional citrate versus systemic heparin for anticoagulation in critically ill patients on continuous venovenous haemofiltration: a prospective randomized multicentre trial
    Gerd R Hetzel
    Department of Nephrology, Medical Faculty, Heinrich Heine University Dusseldorf, Germany
    Nephrol Dial Transplant 26:232-9. 2011
    ..Several protocols avoiding heparin and using regional citrate anticoagulation have been developed to diminish bleeding risks...
  67. ncbi Therapeutic use of heparin beyond anticoagulation
    Ralf J Ludwig
    Department of Dermatology, University of Lubeck, Ratzeburger Allee 160, 23562 Lübeck, Germany
    Curr Drug Discov Technol 6:281-9. 2009
    b>Heparin has been used as an anticoagulant for decades. Recently, attention has been drawn to the non-anticoagulant activities of heparin...
  68. pmc Effects of heparin and related molecules upon neutrophil aggregation and elastase release in vitro
    Rachel A Brown
    Sackler Institute of Pulmonary Pharmacology, Guy s, King s and St Thomas School of Biomedical Sciences, King s College London, London
    Br J Pharmacol 139:845-53. 2003
    ..b>Heparin inhibits the enzymatic activity of elastase and here we provide evidence for the first time that heparin can ..
  69. ncbi Identification of fibroblast growth factor 9 (FGF9) as a high affinity, heparin dependent ligand for FGF receptors 3 and 2 but not for FGF receptors 1 and 4
    D Hecht
    Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel
    Growth Factors 12:223-33. 1995
    Fibroblast growth factors (FGF) are multifunctional, heparin binding polypeptides that share structural similarity, but differ in their target cell specificity and expression pattern...
  70. ncbi Up-regulation of EGF receptor and its ligands, AREG, EREG, and HB-EGF in oral lichen planus
    Kenichi Kumagai
    Department of Oral and Maxillofacial Surgery, Tsurumi University, Tsurumi ku, Yokohama, Japan
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 110:748-54. 2010
    ..This study aimed to investigate the roles of the epidermal growth factor receptor (EGFR) family members and their ligands in oral lichen planus (OLP)...
  71. ncbi Characterization of the interaction between tumor necrosis factor-stimulated gene-6 and heparin: implications for the inhibition of plasmin in extracellular matrix microenvironments
    David J Mahoney
    Medical Research Council Immunochemistry Unit, University of Oxford, United Kingdom
    J Biol Chem 280:27044-55. 2005
    ..Here we show that this domain can also associate with the glycosaminoglycan heparin/heparan sulfate...
  72. pmc Clinical review: anticoagulation for continuous renal replacement therapy--heparin or citrate?
    Heleen M Oudemans-van Straaten
    Department of Intensive Care Medicine, Onze Lieve Vrouwe Gasthuis, Oosterpark 9, 1091 AC Amsterdam, The Netherlands
    Crit Care 15:202. 2011
    b>Heparin is the most commonly prescribed anticoagulant for continuous renal replacement therapy. There is, however, increasing evidence questioning its safety, particularly in the critically ill...
  73. ncbi Mycoplasma hyopneumoniae Surface proteins Mhp385 and Mhp384 bind host cilia and glycosaminoglycans and are endoproteolytically processed by proteases that recognize different cleavage motifs
    Ania T Deutscher
    NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Camden NSW 2567, Australia
    J Proteome Res 11:1924-36. 2012
    ..b>Heparin- and cilium-binding sites were identified within P60(384), P50(384), and P88(385)...
  74. pmc Contaminated heparin associated with adverse clinical events and activation of the contact system
    Takashi Kei Kishimoto
    Momenta Pharmaceuticals, the Harvard Massachusetts Institute of Technology Division of Health Sciences and Technology, Koch Institute for Integrative Cancer Research, Cambridge, MA 02139, USA
    N Engl J Med 358:2457-67. 2008
    There is an urgent need to determine whether oversulfated chondroitin sulfate (OSCS), a compound contaminating heparin supplies worldwide, is the cause of the severe anaphylactoid reactions that have occurred after intravenous heparin ..
  75. ncbi In vivo distribution and antitumor activity of heparin-stabilized doxorubicin-loaded liposomes
    Hee Dong Han
    Medicinal Science Division, Korea Research Institute of Chemical Technology, P O Box 107, Yuseong, Daejeon 305 600, South Korea
    Int J Pharm 313:181-8. 2006
    The purpose of this study was to investigate the effect of heparin conjugation to the surface of doxorubicin (DOX)-loaded liposomes on the circulation time, biodistribution and antitumor activity after intravenous injection in murine ..
  76. pmc Role of cellular glycosaminoglycans and charged regions of viral G protein in human metapneumovirus infection
    Sutthiwan Thammawat
    Department of Microbiology and Infectious Diseases, Flinders Medical Centre, Bedford Park, SA 5042, Australia
    J Virol 82:11767-74. 2008
    ..The pretreatment of hMPV with soluble heparin markedly inhibited the infection of HEp-2 cells...
  77. ncbi Cell transplantation of adipose tissue-derived stem cells in combination with heparin attenuated acute liver failure in mice
    Hiroshi Yukawa
    Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 461 0047, Japan
    Cell Transplant 18:611-8. 2009
    The effect of adipose tissue-derived stem cells (ASCs) in combination with heparin transplantation on acute liver failure mice with carbon tetrachloride (CCl(4)) injection was investigated...
  78. ncbi Characterization of the Mycobacterium avium subsp. paratuberculosis laminin-binding/histone-like protein (Lbp/Hlp) which reacts with sera from patients with Crohn's disease
    Louise H Lefrançois
    INRA, UR1282, Infectiologie Animale, Santé Publique IASP 311, F 37380 Nouzilly, France
    Microbes Infect 13:585-94. 2011
    ..A Map protein was isolated by heparin-Sepharose chromatography and identified as a laminin-binding protein like...
  79. pmc Structural basis for selective vascular endothelial growth factor-A (VEGF-A) binding to neuropilin-1
    Matthew W Parker
    Department of Molecular and Cellular Biochemistry and Center for Structural Biology, University of Kentucky, Lexington, Kentucky 40536, USA
    J Biol Chem 287:11082-9. 2012
    ..We have determined the crystal structure of the exon 7/8-encoded VEGF-A heparin binding domain in complex with the Nrp1-b1 domain...
  80. ncbi Antimetastatic activities of heparins and modified heparins. Experimental evidence
    Lubor Borsig
    Institute of Physiology, University of Zürich and Zürich Center for Integrative Human Physiology, Zurich, Switzerland
    Thromb Res 125:S66-71. 2010
    b>Heparin is commonly used for prevention or treatment of cancer-associated thromboembolism. Recent clinical evidence indicates that heparin, and low-molecular weight heparin improves survival of cancer patients...
  81. ncbi Heparin inhibits ligand binding to the leukocyte integrin Mac-1 (CD11b/CD18)
    K Peter
    Department of Internal Medicine III, University of Heidelberg, Germany
    Circulation 100:1533-9. 1999
    The clinical benefits of heparin reach beyond its anticoagulative properties. Recently, it has been described that leukocytes adhere on immobilized heparin mediated by the integrin Mac-1 (CD11b/CD18, alphaMbeta2, or CR3)...
  82. pmc Identification of a linear heparin binding domain for human respiratory syncytial virus attachment glycoprotein G
    S A Feldman
    Laboratory of Pediatric and Respiratory Virus Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
    J Virol 73:6610-7. 1999
    ..Infection is mediated, in part, by an initial interaction between attachment protein (G) and a highly sulfated heparin-like glycosaminoglycan (Gag) located on the cell surface...
  83. ncbi Semi-rigid solution structures of heparin by constrained X-ray scattering modelling: new insight into heparin-protein complexes
    Sanaullah Khan
    Department of Structural and Molecular Biology, Division of Biosciences, Darwin Building, University College London, Gower Street, London WC1E 6BT, UK
    J Mol Biol 395:504-21. 2010
    The anionic polysaccharides heparin and heparan sulphate play essential roles in the regulation of many physiological processes. Heparin is often used as an analogue for heparan sulphate...
  84. pmc Recent progress and applications in glycosaminoglycan and heparin research
    Tatiana N Laremore
    Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
    Curr Opin Chem Biol 13:633-40. 2009
    b>Heparin, the focus of this review, is a crucially important anticoagulant drug produced from animal sources, which was contaminated last year leading to a number of adverse side effects, some resulting in death...
  85. pmc The structure of the glial cell line-derived neurotrophic factor-coreceptor complex: insights into RET signaling and heparin binding
    Vimal Parkash
    Institute of Biotechnology, University of Helsinki, Helsinki, Finland
    J Biol Chem 283:35164-72. 2008
    ..We have solved the GDNF(2).GFR alpha 1(2) complex structure at 2.35 A resolution in the presence of a heparin mimic, sucrose octasulfate. The structure of our GDNF(2)...
  86. ncbi The characteristics, biodistribution and bioavailability of a chitosan-based nanoparticulate system for the oral delivery of heparin
    Mei Chin Chen
    Department of Chemical Engineering, National Cheng Kung University, Tainan City, Taiwan, ROC
    Biomaterials 30:6629-37. 2009
    b>Heparin is a potent anticoagulant; however, it is poorly absorbed in the gastrointestinal tract...
  87. ncbi Heparin and heparan sulfate: structure and function
    Dallas L Rabenstein
    Department of Chemistry, University of California, Riverside 92521, USA
    Nat Prod Rep 19:312-31. 2002
    This review covers the structure and function of heparin and heparan sulfate glycosaminoglycans...
  88. pmc Heparin-degrading sulfatases in hepatocellular carcinoma: roles in pathogenesis and therapy targets
    Jin Ping Lai
    Center for Basic Research in Digestive Diseases, Miles and Shirley Fiterman Center for Digestive Diseases, Mayo Clinic College of Medicine and Mayo Clinic Cancer Center, 200 First Street, SW, Rochester, MN 55905, USA
    Future Oncol 4:803-14. 2008
    ..Two recently identified human heparin-degrading endosulfatases, named sulfatase 1 (SULF1) and sulfatase 2 (SULF2), have been found to be involved in ..
  89. pmc Quantitative characterization of heparin binding to Tau protein: implication for inducer-mediated Tau filament formation
    Hai Li Zhu
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
    J Biol Chem 285:3592-9. 2010
    ..Polyanionic cofactors such as heparin can induce Tau filament formation in vitro...
  90. ncbi Roles of heparan-sulphate glycosaminoglycans in cancer
    Ram Sasisekharan
    Biological Engineering Division and Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Rev Cancer 2:521-8. 2002
  91. ncbi Heparin-like glycosaminoglycans prevent the infection of measles virus in SLAM-negative cell lines
    Yuri Terao-Muto
    Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo, 4 6 1 Sirokanedai, Minato ku, Tokyo 108 8639, Japan
    Antiviral Res 80:370-6. 2008
    ..Soluble heparin (HP) inhibited the rMV-EGFP infectivity in SLAM-negative cell lines in a dose-dependent manner...
  92. pmc Antithrombin-S195A factor Xa-heparin structure reveals the allosteric mechanism of antithrombin activation
    Daniel J D Johnson
    Department of Haematology, Division of Stuctural Medicine, Thrombosis Research Unit, Cambridge Institute for Medical Research, University of Cambridge, UK
    EMBO J 25:2029-37. 2006
    ..One of the principal regulatory mechanisms involves heparin activation of the serpin antithrombin (AT)...
  93. ncbi Heparin, heparan sulfate and heparanase in inflammatory reactions
    Jin ping Li
    Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden
    Thromb Haemost 102:823-8. 2009
    ..b>Heparin, a classical anticoagulant, exhibits anti-inflammatory effects in animal models and in the clinic, presumably ..
  94. ncbi Structure of heparin-binding EGF-like growth factor. Multiple forms, primary structure, and glycosylation of the mature protein
    S Higashiyama
    Department of Surgical Research, Children s Hospital, Boston, Massachusetts
    J Biol Chem 267:6205-12. 1992
    b>Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a newly described member of the epidermal growth factor (EGF) family that is mitogenic for BALB/c 3T3 cells, inhibits the binding of 125I-EGF to its receptor, and ..
  95. ncbi Comparison of effects on markers of blood cell activation of enoxaparin, dalteparin, and unfractionated heparin in patients with unstable angina pectoris or non-ST-segment elevation acute myocardial infarction (the ARMADA study)
    Gilles Montalescot
    Institut de Cardiologie, Bureau 2 236, Groupe Hospitalier Pitié Salpêtrière Hospital, AP HP, 47 Boulevard de l Hopital, 75013 Paris, France
    Am J Cardiol 91:925-30. 2003
    ..LMWHs) enoxaparin and dalteparin have shown superior and equivalent efficacy, respectively, over unfractionated heparin (UFH) in patients with unstable angina pectoris (UAP) or non-ST-segment elevation myocardial infarction (NSTEMI)...
  96. ncbi Assessment of the heparin-binding AN69 ST hemodialysis membrane: II. Clinical studies without heparin administration
    Sylvie Lavaud
    Service de Nephrologie, Centre Hospitalier et Universitaire and CNRS FRE 2534, Reims, France
    ASAIO J 51:348-51. 2005
    Binding polyanionic unfractionated heparin over the modified AN69 polyacrylonitrile membrane, the surface electronegativity of which has been neutralized by polyethyleneimine (AN69-ST), renders the membrane more hemocompatible...
  97. ncbi Heparin-binding EGF-like growth factor: characterization of rat and mouse cDNA clones, protein domain conservation across species, and transcript expression in tissues
    J A Abraham
    Scios Nova Inc, Mountain View, CA 94043
    Biochem Biophys Res Commun 190:125-33. 1993
    Clones were obtained that encode the rat and mouse forms of heparin-binding EGF-like growth factor (HB-EGF), a potent mitogen for smooth muscle cells, fibroblasts and keratinocytes that is proposed to be derived from a transmembrane ..
  98. ncbi Heparin attenuates cytotoxic and inflammatory activity of Alzheimer amyloid-beta in vitro
    Luigi Bergamaschini
    Department of Internal Medicine, Ospedale Maggiore IRCCS, University of Milan, Italy
    Neurobiol Aging 23:531-6. 2002
    ..We evaluated the effect of heparin on Abeta (1-42) neurotoxicity and on its ability to activate complement and contact system...
  99. ncbi Low anticoagulant heparin targets multiple sites of inflammation, suppresses heparin-induced thrombocytopenia, and inhibits interaction of RAGE with its ligands
    Narayanam V Rao
    Department of Internal Medicine, University of Utah Medical Center, Salt Lake City, UT 84132, USA
    Am J Physiol Cell Physiol 299:C97-110. 2010
    While heparin has been used almost exclusively as a blood anticoagulant, important literature demonstrates that it also has broad anti-inflammatory activity...
  100. ncbi Delineating mechanisms of dissociation for isomeric heparin disaccharides using isotope labeling and ion trap tandem mass spectrometry
    Ola M Saad
    Department of Chemistry, University of California at Berkeley, Berkeley, California 94720 1460, USA
    J Am Soc Mass Spectrom 15:1274-86. 2004
    b>Heparin and heparan sulfate (HS) glycosaminoglycans have been identified as important players in many physiological as well as pathophysiological settings...
  101. ncbi A novel anti-angiogenic form of antithrombin with retained proteinase binding ability and heparin affinity
    H Larsson
    Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden
    J Biol Chem 276:11996-2002. 2001
    Latent antithrombin, an inactive antithrombin form with low heparin affinity, has previously been shown to efficiently inhibit angiogenesis and tumor growth...

Research Grants73

  1. Anti-Inflammatory Glycosaminoglycan Ethers for Treatment of Periodontitis
    JUSTIN RODNEY SAVAGE; Fiscal Year: 2011
    ..Sulfated glycosaminoglycans (GAGs, e.g., heparin) block ligation of RAGE by AGE and its other ligands...
  2. Cyr61/CCN1-Induced Angiogenesis and Vasculogenesis in the Retina
    Brahim Chaqour; Fiscal Year: 2010
    ..work in the PI's laboratory have revealed that the cysteine-rich protein 61 (Cyr61), a novel ECM-associated, heparin- and integrin-binding protein, stimulates the angiogenic phenotype of the BM-derived hematopoeitic progenitors, ..
  3. Hepatic Lipase and HCV Infection
    Tianyi Wang; Fiscal Year: 2010
    ..enzymes in maintaining circulating lipoprotein levels and can facilitate lipid uptake via their associations with heparin sulphate proteoglycans (HSPG) on hepatocyte surface...
    Douglas M Tollefsen; Fiscal Year: 2010
    b>Heparin cofactor II (HCII) is an inhibitor of thrombin in plasma that can be activated by dermatan sulfate (DS) proteoglycans on vascular smooth muscle cells and fibroblasts...
  5. Adjunctive Use of Apyrase to Fibrinolytic Therapy
    Ridong Chen; Fiscal Year: 2013
    ..The current regimen, including tissue plasminogen activator, aspirin, clopidogrel and heparin, still induces inadequate coronary reperfusion in 30-40% of patients and early thrombotic reocclusion in 5-10% ..
  6. Novel Glycosaminoglycan Ethers for Prevention of Metastasis
    Alana L Welm; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Heparin and its derivatives block P- and L-selectin mediated metastatic spread of cancer in animal models, and they potently inhibit the matrix degrading enzyme heparanase...
  7. GPCR Signaling &Vascular Wall Remodeling
    Gadiparthi N Rao; Fiscal Year: 2013
    ..While protease-dependent shedding of heparin-binding growth factors, in this case, heparin-binding epidermal growth factor (HB-EGF), appears to be accountable ..
  8. Extracellular Matrix Remodeling During Aging
    JANE M SOTTILE; Fiscal Year: 2010
    ..the deposition and turnover of other ECM molecules including collagen I, collagen III, thrombospondin, and heparin sulfate proteoglycans...
    Jeffrey H Miner; Fiscal Year: 2012
    ..are laminin-521 (alpha5beta2gamma1), type IV collagen (the alpha3/4/5 network), nidogens-1 and -2, and the heparin sulfate proteoglycan agrin...
  10. Molecular mechanism of antiangiogenic properties of gold nanoparticle
    Priyabrata Mukherjee; Fiscal Year: 2013
    ..We have recently demonstrated that "bare" AuNPs bind to heparin binding growth factors such as VPF/VEGF165, bFGF and PlGF through their heparin-binding domain and inhibit their ..
  11. Heparan Sulfate Proteoglycans in Human Hematopoiesis
    Pankaj Gupta; Fiscal Year: 2013
    ..Gremlin1 is an extracellular heparin-binding BMP inhibitor that plays a critical role in growth, differentiation and embryonic development and in ..
  12. Novel Glycosaminoglycan Derivatives for Treatment of Bladder Inflammation
    JUSTIN RODNEY SAVAGE; Fiscal Year: 2011
    ..First, heparin is administered intravesically, but the anti-coagulant properties and expense limit its regular usage...
  13. Dysregulation of the Tumor Microenvironment in Hepatocellular Carcinoma
    Lewis R Roberts; Fiscal Year: 2010
    ..Stellate cell activation is dependent on signaling by platelet-derived growth factor (PDGF), a heparin-binding growth factor, and transforming growth factor-[unreadable] (TGF-[unreadable]) which is stored in the ECM ..
  14. New Pathways in Thrombosis and Inflammation Mediated by Semaphorin-Plexin Signali
    Guy A Zimmerman; Fiscal Year: 2010
    ..therapies including cyclooxygenase inhibitors (aspirin), thienopyridines (clopidogrel), and anticoagulants (heparin, warfarin)...
  15. Novel Biomarkers Predictive of Heparin-Induced Thrombocytopenia
    Barbara A Konkle; Fiscal Year: 2010
    ..Thrombosis is the major cause of death in the United States and heparin, in unfractionated (UFH) or low molecular weight form, is uniformly used for anticoagulation in the hospital ..
  16. Insulin Resistance in Chronic Heart Failure: pathophysiology and potential for re
    Nir Uriel; Fiscal Year: 2013
    ..We will also measure post heparin plasma lipoprotein lipase (LPL) activity to assess the availability of circulating lipoprotein-derived FFA in ..
  17. The role of Histidine rich protein II in cerebral malaria
    Priya Pal; Fiscal Year: 2013
    ..Previous studies from our laboratory show that HRPII binds to soluble glycosaminoglycans (GAGs) such as heparin, heparan sulfate, and dermatan sulfate with high affinity...
  18. Role of Maternal Platelets in the Placenta and Pregnancy Complications
    Rashmi Sood; Fiscal Year: 2013
    ..find that this model is driven by thrombin-mediated platelet activation and that pregnancy outcome is improved by heparin treatment, but not by treatment with hirudin, fondaparinux or a direct Xa inhibitor...
  19. Metabolic Engineering of Chinese Hamster Ovary Cells for Production of Heparin
    Susan T Sharfstein; Fiscal Year: 2011
    b>Heparin is the most widely used anticoagulant drug in modern medicine...
  20. The central role of c-Met in H. pylori pathogenesis.
    Sarah E Keates; Fiscal Year: 2010
    ..Disintegrin And Metallopeptidase domain-17 (ADAM-17);and that iii) activation of ADAM-17 can mediate shedding of heparin-binding epidermal growth factor (HB-EGF) which in turn culminates in EGFR transactivation...
  21. Cell-synthesized Thread-based Tissue Engineering
    NICOLAS L'HEUREUX; Fiscal Year: 2012
    ..As a cost-effective alternative, we will explore the possibility of replacing it by a new type of heparin coating...
  22. Physiology and Molecular Biology of Factor XI
    David Gailani; Fiscal Year: 2013
    ..In Aim 3, we will determine the importance of the fXI anion binding sites to heparin-dependent regulation of fXIa by plasma serpins, and evaluate the capacity of poly-P to down-regulate serpin-..
  23. Apolipoprotein A-V: A Functional Proteomics Study
    ROBERT O apos MARA RYAN; Fiscal Year: 2010
    ..553G>T SNP will be characterized for intra- molecular disulfide bond formation, heparin / receptor binding interactions and in vivo TG modulation capability...
  24. GE Healthcare Fast Protein Liquid Chromatography FPLC product number 13470501.
    Jasti S Rao; Fiscal Year: 2010
    ..b>heparin for AAV2 and AAV6). The packing material used, called the affinity matrix, must be inert and easily modified...
  25. Designing a Microenvironment Niche for Liver-Specific Differentiation of hESCs
    Alexander Revzin; Fiscal Year: 2013
    ..Stem cell-carrying heparin hydrogel microstructures will be implanted in immunodeficient mice and survival/function of cells within these ..
    Yvonne Kapila; Fiscal Year: 2009
    ..We have determined that the carboxyl-terminal heparin-binding domain and alternatively spliced V region of FN are important to this process, since a FN miniprotein (V+..
  27. Extracellular Sulfatases As Novel Biomarkers For Early Detection of Cancer
    Joanna Phillips; Fiscal Year: 2013
    ..Aim 4: Characterize the plasma levels of heparin-binding factors (growth factors, morphogens, cytokines, chemokines) that are potentially mobilized by SULF action ..
  28. Extracellular Matrix Components, Oxidants and Antioxidants in Pulmonary Fibrosis
    CORRINE R KLIMENT; Fiscal Year: 2010
    ..the lung from oxidant-induced damage, inflammation, and fibrosis is by preventing oxidative fragmentation of Heparin/Heparan Sulfate (HS) in the ECM...
    Robert J Linhardt; Fiscal Year: 2012
    ..e., chondroitin, dermatan, heparin, etc.), molecular weight, charge density and disaccharide analysis...
    Steven T Olson; Fiscal Year: 2013
    ..recent studies have suggested an important revision of the allosteric mechanism of activation of this serpin by heparin in showing that activation is mediated principally by the relief of repulsive interactions with factor Xa and ..
    Peter J Newman; Fiscal Year: 2013
    ..Wang, seeks to define the role of specific B lymphocyte populations in the cellular immune response underlying heparin-induced thrombocytopenia - studies that may transform our ability to diagnose and manage this complex bleeding ..
  32. Role of heparin binding growth factors in vascular leakage and fatal bleeding
    Patricio E Ray; Fiscal Year: 2012
    Very little is known about the role that circulating heparin binding angiogenic growth factors play in the pathogenesis of bleeding disorders induced by heparin-like drugs or heparinoids in critically ill children...
  33. Reduction of Tunneled Dialysis Catheter Dysfunction Via Long Term Nitric Oxide Re
    Prabir Roy-Chaudhury; Fiscal Year: 2013
    ..47,000 to the cost of care and a 14.6 d increase in length of stay. Current heparin, silver impregnated, or antibiotic coated TDCs are either expensive, non-effective, or suffer from mechanical ..
  34. Serpin Regulation of Coagulation Proteases
    Alireza R Rezaie; Fiscal Year: 2013
    ..AT is a relatively weak inhibitor of coagulation proteases unless it binds to the heparin-like glycosaminoglycans lining the microvasculature or therapeutically administered for prophylaxis and treatment ..
  35. Factor XI inhibitor for thrombosis
    ERIK IAN TUCKER; Fiscal Year: 2013
    ..significant antithrombotic effects that are comparable to a high interventional dose of the low-molecular-weight heparin enoxaparin (Lovenox(R), 1mg/kg, iv), and 3) Verifying that the antithrombotic dose of AXIMAB 1A6 produces no ..
  36. Structure-Function of AAV - a Viral Gene Therapy Vector
    Michael S Chapman; Fiscal Year: 2013
    ..to characterize the diversity of HS interactions that has evolved in AAV, correlating structure with measured heparin affinity, cell attachment and cell entry, measured by in vitro transgene expression...
  37. Regulation and Function of Immunoglobulin D in Mucosal Immune Defense
    Andrea Cerutti; Fiscal Year: 2013
    ..by activating local and systemic innate immune cells such as basophils and mast cells through interaction with heparin, eosinophil cationic protein and inflammasomes. Three aims are proposed...
  38. Heparan sulfate proteoglycans in aging and development
    GREGORY COLE; Fiscal Year: 2009
    ..These studies will focus on analyzing the role agrin plays in modulating the function of heparin-binding signaling molecules such as the fibroblast growth factors (FGFs)...
  39. 2012 New Insights into Coagulation
    Eleanor Pollak; Fiscal Year: 2012
    ..Other topics that we plan to hear discussed include the older well-established coagulation agents: heparin and low-molecular weight heparin, warfarin, and intravenous direct thrombin inhibitors...
  40. Therapeutic factor XI blockade for sepsis
    ERIK IAN contact TUCKER; Fiscal Year: 2010
    ..by antibody inhibition of FXI is antithrombotic for more than a week, and hemostatically safer than heparin or aspirin...
  41. A murine model for human factor H R1210C mutation-related diseases
    Wenchao Song; Fiscal Year: 2013
    ..The R1210C variant of human fH showed reduced binding to C3b/C3d, heparin and endothelial cells, yet R1210 is not conserved across species (D1210 in mouse, G1210 in rat, P1210 in cows and ..
  42. HEPylated G-CSF: Drug with Safer, Enhanced Delivery for Neutropenia Treatment
    Wei Jing; Fiscal Year: 2011
    ..Heparosan, a new type of therapeutic modifying agent, is a natural "self" polysaccharide related to heparin. Caisson's technology also allows for synthesis of sugar polymers with a very narrow size distribution and ..
  43. Pregnancy specific glycoprotein 1 activates transforming growth factor beta
    Gabriela S Dveksler; Fiscal Year: 2013
    ..Recently, we described that besides its immune regulatory function, PSG1 is pro-angiogenic. PSG1 binds to the heparin and chondroitin sulfate in syndecans and this interaction results in the formation of capillary-like structures ..
  44. Microthrombus Formation Triggers Lung Injury in Sepsis
    BRUCE ALLAN HARMS; Fiscal Year: 2012
    ..of thrombus formation prevents neutrophil sequestration, and prevents lung injury: low molecular weight heparin, recombinant hirudin, and recombinant activated protein C have anti-thrombotic properties that prevent ..
  45. Genetic influences in experimental pancreatitis
    Bishr Omary; Fiscal Year: 2013
    ..to experimental pancreatitis (Aim 3);and (iii) Testing the effect of targeting the coagulation pathway, via heparin, as a therapeutic modality in experimental pancreatitis (Aim 4)...
  46. Pathogenesis of HIV-HUS in children
    Patricio E Ray; Fiscal Year: 2012
    ..In aim 1 we will define how FGF-2, BP-1, HIV-Tat , VEGF-A, and heparin, alone or in combination, modulate the permeability of cultured human glomerular endothelial cells and determine ..
  47. Improving the diagnosis of heparin-induced thrombocytopenia
    Adam Cuker; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Heparin-induced thrombocytopenia (HIT) is a potentially fatal prothrombotic complication of heparin therapy...
  48. LPD Nanoparticles in Anti-Cancer Therapy
    Leaf Huang; Fiscal Year: 2012
    ..In aim 3, we will employ another nanoparticle formulation, i.e., LPH, which contains heparin sulfate to deliver doxorubicin (dox), a potent anti-cancer drug, either by itself or together with EGFR siRNA...
  49. Mechanisms of normal and abnormal platelet homeostasis
    Joel S Bennett; Fiscal Year: 2010
    ..Project 1, entitled "Understanding the mechanistic basis of heparin-induced thrombocytopenia (HIT)", tests the hypothesis that variations in platelet factor 4 (PF4) secretion are ..
    UMESH RAMANLAL DESAI; Fiscal Year: 2013
    ..with proteins using computational, biochemical and biophysical technologies;designing specific GAG activators of heparin cofactor II;and developing selected GAGs as clinically useful anticoagulants;Project III (PL: K...
  51. The Role of the EGF Receptor in Acute and Chronic Renal Injury
    Raymond Harris; Fiscal Year: 2013
    ..the current funding period have indicated that the membrane-associated form of EGFR ligands, and specifically heparin-binding EGF-like growth factor (HB-EGF), subserves different functions than the soluble mature form of the ..
  52. Biomimetic Engineering of Vascular Prostheses
    Roger E Marchant; Fiscal Year: 2012
    ..for 1v23 (compared to 1IIb23), REDV peptide with specificity for 1421, CRRETAWAC peptide specific for 1521, and heparin binding peptides that demonstrate specificity for syndecans...
  53. Saturated Fat and Protein Effects on Atherogenic Dyslipidemia
    Ronald M Krauss; Fiscal Year: 2013
    ..for possible metabolic determinants of dietary effects on apoB-containing lipoprotein subclasses, including post-heparin plasma hepatic lipase activity, which is critical for production of smaller LDL, and LDL receptor activity as ..
  54. Potential of a dominant-negative FGF mutant as a therapeutic in cancer
    Yoshikazu Takada; Fiscal Year: 2012
    ..an FGF1 mutant (Arg-50 to Glu, R50E) that is defective in integrin binding but still binds to FGF-receptor or heparin. R50E was defective in inducing FGF signaling while it binds to heparin or FGFR, suggesting that integrin binding ..
  55. GPx-3 and Peroxide Flux in the Endothelial Cell
    Joseph Loscalzo; Fiscal Year: 2013
    ..with complex and unique translational requirements;demonstration of the cofactor activation of GPx-3 catalysis by heparin via a unique polyanionic glycosaminoglycan binding site in the solvent-exposed carboxyterminal tail;the ..
  56. Identification of novel genes regulating metalloproteinase activity
    Andreas Herrlich; Fiscal Year: 2009
    ..In the kidney, the EGF ligand HB (heparin-binding)-EGF is involved in tubular repair after injury...
  57. Hypertensive Mechanisms in Preeclampsia
    ERIC MATTHEW GEORGE; Fiscal Year: 2013
    ..One potential mechanism is suggested through the presence of a heparin binding site on the sFlt-1 protein and recent evidence from the literature that the switch between local ..
  58. Development of B-Lock an antibiofilm catheter lock product
    Krzysztof Appelt; Fiscal Year: 2010
    ..Conduct a randomized, double-blind clinical trial to evaluate B-LockTM solution compared with heparin lock solution in the preservation of in-dwelling catheter sterility and potency in patients receiving ..
  59. Regulation of heparanase in cancer progression
    Israel Vlodavsky; Fiscal Year: 2013
    ..e., chemically modified non-anticoagulant species of heparin, siRNA), antibodies directed against specific domains of the heparanase protein, assay systems (i.e...
  60. "New Needle for Two-Needle Hemodialysis"
    ALFREDO R ZARATE; Fiscal Year: 2011
    ..HD prescription during both HD including length of dialysis, type of dialyzer non-reused, size of needles (15g), heparin, dialysate composition, and blood and dialysate flow rate...
  61. Structural and Biophysical Characterization of Hedgehog Signaling
    Daniel J Leahy; Fiscal Year: 2013
    ..Hh:Cdo, and Hh:Boc complexes, and demonstrated that these interactions depend on additional co-factors including heparin and Ca2+. We have also recently expressed and purified small amounts (~40 ug) of Patched...
  62. Structural and functional characterization of the receptor protein tyrosine phosp
    Heidi Erlandsen; Fiscal Year: 2010
    ..has previously been described mainly in neuronal cells, and was the first described receptor for the heparin-binding molecule pleiotrophin (PTN), a small chemokine of 15- 17 kDa, that was also independently identified as ..
  63. Long Circulating Low Molecular Weight Heparins Pulmonary
    Fakhrul Ahsan; Fiscal Year: 2006
    ..Drugs that have traditionally been used for the short and long term treatment of VTE include unfractionated heparin and warfarin...
    Joseph Toce; Fiscal Year: 1991
    ..Abstract): This is a phase I application in which the goal is to develop a therapeutic agent which would replace heparin. Although heparin is the best drug for treatment of thromboembolic disease, it is unstable if administered orally ..
  65. Structural Mechanisms for the Inhibition of Thrombosis
    JAMES HUNTINGTON; Fiscal Year: 2009
    ..inhibition of upstream coagulation factor Xa;2) direct inhibition of thrombin by serpins antithrombin (AT) and heparin cofactor II (HCII);and, 3) attenuation of thrombin's procoagulant activity through cofactor binding and ..
  66. Mechanisms of Hemostatic Protease Inhibition by Serpins
    Ingrid M Verhamme; Fiscal Year: 2010
    The long-term goal is to define the molecular mechanisms of thrombin (T) inhibition by the serpins, heparin cofactor II (HCII) and plasminogen activator inhibitor-1 (PAI-1), implicated in arterial thrombosis...
    GIAN VISENTIN; Fiscal Year: 2000
    b>Heparin-induced thrombocytopenia/thrombosis (HITP) is an important cause of morbidity and mortality in patients receiving heparin therapy...
  68. Etiologic and therapeutic studies of heparin-induced thrombocytopenia (HIT)
    Mortimer Poncz; Fiscal Year: 2011
    b>Heparin-induced thrombocytopenia (HIT) is an iatrogenic cause of thrombocytopenia and thrombosis due to antibodies to complexes between Platelet Factor 4 (PF4) and heparin...
  69. Antithrombotic Activity of Ascidian Glycosaminoglycans
    DOUGLAS TOLLEFSEN; Fiscal Year: 2004
    DESCRIPTION (provided by applicant) Heparin from porcine or bovine tissues is used to treat diseases such as venous thrombosis, pulmonary embolism, and coronary artery thrombosis...
    Scott Cooper; Fiscal Year: 2003
    ..Previously we made the observation that, unlike AT, PCI is a potent inhibitor of thrombin bound to TM. The heparin binding domains of PCI and AT also differ; in AT the D-helix is a major part of the heparin-binding domain, while ..
  71. Biomedical Polymers With Heparin-Binding End Groups
    Robert Ward; Fiscal Year: 2004
    Endpoint immobilization of heparin on the surface of polymeric biomaterials is a well-established method for improving thrombo-resistance...
  72. Development of biomimetic oligomers as anti-coagulant antagonists
    Richard Scott; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): Unfractionated heparin (UFH) and the low molecular weight heparins (LMWHs) are widely used anti-coagulants employed in a number of clinical and surgical applications...
    Kevin Mayo; Fiscal Year: 1992
    Platelet factor (PF4) and B-thromboglobulin-related proteins (BTG) are low- molecular weight heparin binding proteins generally considered to be platelet specific and are involved in blood clotting, wound healing, tissue repair and cell ..