dihydroxyacetone

Summary

Summary: A ketotriose compound. Its addition to blood preservation solutions results in better maintenance of 2,3-diphosphoglycerate levels during storage. It is readily phosphorylated to dihydroxyacetone phosphate by triokinase in erythrocytes. In combination with naphthoquinones it acts as a sunscreening agent.

Top Publications

  1. ncbi Effect of pyruvate and dihydroxyacetone on metabolism and aerobic endurance capacity
    J L Ivy
    Department of Kinesiology and Health Education, University of Texas at Austin, USA
    Med Sci Sports Exerc 30:837-43. 1998
  2. ncbi Disruption of the membrane-bound alcohol dehydrogenase-encoding gene improved glycerol use and dihydroxyacetone productivity in Gluconobacter oxydans
    Hiroshi Habe
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology AIST
    Biosci Biotechnol Biochem 74:1391-5. 2010
  3. doi Expression of Vitreoscilla hemoglobin enhances cell growth and dihydroxyacetone production in Gluconobacter oxydans
    Minghua Li
    State Key Laboratory of Bioreactor Engineering, Institute of Newworld Biotechnology, East China University of Science and Technology, Shanghai, China
    Curr Microbiol 61:370-5. 2010
  4. ncbi Study of the inhibitory effect of the product dihydroxyacetone on Gluconobacter oxydans in a semi-continuous two-stage repeated-fed-batch process
    R Bauer
    Institute of Chemical Engineering, Munich University of Technology, Boltzmannstrasse 15, 85747 Garching, Germany
    Bioprocess Biosyst Eng 28:37-43. 2005
  5. doi Use of glycerol for producing 1,3-dihydroxyacetone by Gluconobacter oxydans in an airlift bioreactor
    Zhong Ce Hu
    Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310032, People s Republic of China
    Bioresour Technol 102:7177-82. 2011
  6. pmc Dihydroxyacetone synthase from a methanol-utilizing carboxydobacterium, Acinetobacter sp. strain JC1 DSM 3803
    Y T Ro
    Department of Biology, Yonsei University, Seoul, Korea
    J Bacteriol 179:6041-7. 1997
  7. doi Catalytic conversion of dihydroxyacetone to lactic acid using metal salts in water
    Carolus B Rasrendra
    Chemical Engineering Department, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    ChemSusChem 4:768-77. 2011
  8. ncbi Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans
    D Hekmat
    Institute of Chemical Engineering, Munich University of Technology, Boltzmannstrasse 15, 85747 Garching, Germany
    Bioprocess Biosyst Eng 26:109-16. 2003
  9. doi Formation of pyrazines and a novel pyrrole in Maillard model systems of 1,3-dihydroxyacetone and 2-oxopropanal
    An Adams
    Department of Organic Chemistry, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B 9000 Ghent, Belgium
    J Agric Food Chem 56:2147-53. 2008
  10. ncbi Combining metabolic engineering and adaptive evolution to enhance the production of dihydroxyacetone from glycerol by Gluconobacter oxydans in a low-cost way
    Leifang Lu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Bioresour Technol 117:317-24. 2012

Detail Information

Publications190 found, 100 shown here

  1. ncbi Effect of pyruvate and dihydroxyacetone on metabolism and aerobic endurance capacity
    J L Ivy
    Department of Kinesiology and Health Education, University of Texas at Austin, USA
    Med Sci Sports Exerc 30:837-43. 1998
    Pyruvate and dihydroxyacetone are three carbon compounds that when infused directly into the blood or taken orally produce strong metabolic effects...
  2. ncbi Disruption of the membrane-bound alcohol dehydrogenase-encoding gene improved glycerol use and dihydroxyacetone productivity in Gluconobacter oxydans
    Hiroshi Habe
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology AIST
    Biosci Biotechnol Biochem 74:1391-5. 2010
    b>Dihydroxyacetone (DHA) production from glycerol by Gluconobacter oxydans is an industrial form of fermentation, but some problems exist related to microbial DHA production...
  3. doi Expression of Vitreoscilla hemoglobin enhances cell growth and dihydroxyacetone production in Gluconobacter oxydans
    Minghua Li
    State Key Laboratory of Bioreactor Engineering, Institute of Newworld Biotechnology, East China University of Science and Technology, Shanghai, China
    Curr Microbiol 61:370-5. 2010
    b>Dihydroxyacetone (DHA) is an important ketose sugar, which is extensively used in the cosmetic, chemical, and pharmaceutical industries. DHA has been industrially produced by Gluconobacter oxydans with a high demand of oxygen...
  4. ncbi Study of the inhibitory effect of the product dihydroxyacetone on Gluconobacter oxydans in a semi-continuous two-stage repeated-fed-batch process
    R Bauer
    Institute of Chemical Engineering, Munich University of Technology, Boltzmannstrasse 15, 85747 Garching, Germany
    Bioprocess Biosyst Eng 28:37-43. 2005
    The influence of the product inhibition by dihydroxyacetone (DHA) on Gluconobacter oxydans for a novel semi-continuous two-stage repeated-fed-batch process was examined quantitatively...
  5. doi Use of glycerol for producing 1,3-dihydroxyacetone by Gluconobacter oxydans in an airlift bioreactor
    Zhong Ce Hu
    Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310032, People s Republic of China
    Bioresour Technol 102:7177-82. 2011
    1,3-Dihydroxyacetone can be produced by biotransformation of glycerol with glycerol dehydrogenase from Gluconobacter oxydans cells. Firstly, improvement the activity of glycerol dehydrogenase was carried out by medium optimization...
  6. pmc Dihydroxyacetone synthase from a methanol-utilizing carboxydobacterium, Acinetobacter sp. strain JC1 DSM 3803
    Y T Ro
    Department of Biology, Yonsei University, Seoul, Korea
    J Bacteriol 179:6041-7. 1997
    Acinetobacter sp. strain JC1 DSM 3803, a carboxydobacterium, grown on methanol was found to show dihydroxyacetone synthase, dihydroxyacetone kinase, and ribulose 1,5-bisphosphate carboxylase, but no hydroxypyruvate reductase and very low ..
  7. doi Catalytic conversion of dihydroxyacetone to lactic acid using metal salts in water
    Carolus B Rasrendra
    Chemical Engineering Department, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    ChemSusChem 4:768-77. 2011
    ..on the application of homogeneous catalysts in the form of metal salts on the conversion of trioses, such as dihydroxyacetone (DHA), and glyceraldehyde (GLY) to lactic acid (LA) in water...
  8. ncbi Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans
    D Hekmat
    Institute of Chemical Engineering, Munich University of Technology, Boltzmannstrasse 15, 85747 Garching, Germany
    Bioprocess Biosyst Eng 26:109-16. 2003
    An optimized repeated-fed-batch fermentation process for the synthesis of dihydroxyacetone (DHA) from glycerol utilizing Gluconobacter oxydans is presented...
  9. doi Formation of pyrazines and a novel pyrrole in Maillard model systems of 1,3-dihydroxyacetone and 2-oxopropanal
    An Adams
    Department of Organic Chemistry, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B 9000 Ghent, Belgium
    J Agric Food Chem 56:2147-53. 2008
    ..The model reaction of 20 different amino acids with 1,3-dihydroxyacetone, as a precursor of 2-oxopropanal, was studied by means of SPME-GC-MS to investigate the involvement of the ..
  10. ncbi Combining metabolic engineering and adaptive evolution to enhance the production of dihydroxyacetone from glycerol by Gluconobacter oxydans in a low-cost way
    Leifang Lu
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
    Bioresour Technol 117:317-24. 2012
    Gluconobacter oxydans can rapidly and effectively transform glycerol to dihydroxyacetone (DHA) by membrane-bound quinoprotein sorbitol dehydrogenase (mSLDH)...
  11. ncbi Small substrate, big surprise: fold, function and phylogeny of dihydroxyacetone kinases
    B Erni
    Department of Chemistry and Biochemistry, University of Bern, Switzerland
    Cell Mol Life Sci 63:890-900. 2006
    b>Dihydroxyacetone (Dha) kinases are a family of sequence-conserved enzymes which utilize either ATP (in animals, plants and eubacteria) or phosphoenolpyruvate (PEP, in eubacteria) as their source of high-energy phosphate...
  12. ncbi Microbial production of dihydroxyacetone
    Ruchi Mishra
    Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, 208016 Kanpur, India
    Biotechnol Adv 26:293-303. 2008
    b>Dihydroxyacetone is extensively used in cosmetic industry as an artificial suntan besides having clinical and biological applications...
  13. ncbi An inducible phosphoenolpyruvate: dihydroxyacetone phosphotransferase system in Escherichia coli
    R Z Jin
    J Gen Microbiol 130:83-8. 1984
    A phosphoenolpyruvate: dihydroxyacetone phosphotransferase was induced in Escherichia coli grown on dihydroxyacetone as sole carbon source or in its presence...
  14. ncbi Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway
    J Norbeck
    Department of General and Marine Microbiology, Goteborg University, Medicinaregatan 9 C, 413 90 Goteborg, Sweden
    J Biol Chem 272:5544-54. 1997
    ..We furthermore present evidence for salt induction of glycerol dissimilation via dihydroxyacetone and also identify genes putatively encoding the two enzymes involved; dihydroxyacetone kinase (DAK1 and DAK2) ..
  15. doi Water-compatible organocatalysts for direct asymmetric syn-aldol reactions of dihydroxyacetone and aldehydes
    S S V Ramasastry
    The Skaggs Institute for Chemical Biology, and Departments of Chemistry and Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Org Lett 10:1621-4. 2008
    A novel organocatalyst was developed that effectively catalyzed the reactions of unprotected or protected dihydroxyacetone with a variety of aldehydes to provide syn-aldol products with good yields and ee values up to >99%...
  16. ncbi Dihydroxyacetone metabolism in Salinibacter ruber and in Haloquadratum walsbyi
    Rahel Elevi Bardavid
    The Institute of Life Sciences, and The Moshe Shilo Minerva Center for Marine Biogeochemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
    Extremophiles 12:125-31. 2008
    ..We here identify this product of incomplete glycerol oxidation by Salinibacter as dihydroxyacetone. Genomic information suggests that H...
  17. ncbi Engineering of Saccharomyces cerevisiae for the production of dihydroxyacetone (DHA) from sugars: a proof of concept
    H T T Nguyen
    Department of Microbiology and Genetics, Berlin University of Technology, Seestr 13, D 13353 Berlin, Germany
    Metab Eng 11:335-46. 2009
    b>Dihydroxyacetone (DHA) has numerous industrial applications. In this work, we pursue the idea to produce DHA from sugars in the yeast Saccharomyces cerevisiae, via glycerol as an intermediate...
  18. ncbi Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina
    Janis Liepins
    VTT Biotechnology, Espoo, Finland
    FEBS J 273:4229-35. 2006
    ..the conversion of d-glyceraldehyde and l-glyceraldehyde to glycerol, whereas GLD2 catalyses the conversion of dihydroxyacetone to glycerol. Both enzymes are specific for NADPH as a cofactor...
  19. pmc Antiproliferative effect of dihydroxyacetone on Trypanosoma brucei bloodstream forms: cell cycle progression, subcellular alterations, and cell death
    Nestor L Uzcategui
    Interfaculty Institute of Biochemistry, University of Tuebingen, Germany
    Antimicrob Agents Chemother 51:3960-8. 2007
    We evaluated the effects of dihydroxyacetone (DHA) on Trypanosoma brucei bloodstream forms. DHA is considered an energy source for many different cell types. T. brucei takes up DHA readily due to the presence of aquaglyceroporins...
  20. doi Dihydroxyacetone: a safe camouflaging option in vitiligo
    Natta Rajatanavin
    Division of Dermatology, Department of Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, 270 Rama VI Road, Bangkok 10400, Thailand
    Int J Dermatol 47:402-6. 2008
    ..Most treatment protocols for vitiligo usually do not result in complete repigmentation. Therefore, cosmetically acceptable camouflage, low cost and easy to handle alternatives are warranted...
  21. ncbi Dihydroxyacetone induced autophagy in African trypanosomes
    Nestor L Uzcategui
    Interfaculty Institute of Biochemistry, University of Tubinge, Tubinge, Germany
    Autophagy 3:626-9. 2007
    b>Dihydroxyacetone (DHA) was examined to explore its trypanocidal activity. The compound is easily taken up by trypanosomes via its aquaglyceroporins but is not converted to a glycolytic intermediate due to the lack of a respective kinase...
  22. ncbi Enhancement of arm exercise endurance capacity with dihydroxyacetone and pyruvate
    R T Stanko
    Department of Medicine, Montefiore Hospital, Pittsburgh, Pennsylvania 15213
    J Appl Physiol (1985) 68:119-24. 1990
    The effects of dietary supplementation of dihydroxyacetone and pyruvate (DHAP) on endurance capacity and metabolic responses during arm exercise were determined in 10 untrained males (20-26 yr)...
  23. doi Role of GldA in dihydroxyacetone and methylglyoxal metabolism of Escherichia coli K12
    Krishna P Subedi
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yusong Ku, Taejon, Korea
    FEMS Microbiol Lett 279:180-7. 2008
    The metabolic pathway involving dihydroxyacetone is poorly characterized although novel enzymes associated with this metabolite have recently been demonstrated...
  24. ncbi Transaldolase B: trapping of Schiff base intermediate between dihydroxyacetone and epsilon-amino group of active-site lysine residue by borohydride reduction
    Gunter Schneider
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Methods Enzymol 354:197-201. 2002
  25. ncbi Alterations of the glucose metabolism in a triose phosphate isomerase-negative Saccharomyces cerevisiae mutant
    C Compagno
    Dipartimento di Fisiologia e Biochimica Generali, Sezione Biochimica Comparata, Universita degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy
    Yeast 18:663-70. 2001
    The absence of triose phosphate isomerase activity causes an accumulation of only one of the two trioses, dihydroxyacetone phosphate, and this produces a shift in the final product of glucose catabolism from ethanol to glycerol (Compagno ..
  26. ncbi Role of botrytized grape micro-organisms in SO2 binding phenomena
    J C Barbe
    , , Talence Cedex, France
    J Appl Microbiol 90:34-42. 2001
    ..produce gluconic acid (in balance with delta-gluconolactone) from glucose, 5-oxofructose from fructose and dihydroxyacetone from glycerol...
  27. ncbi Contribution of L-3,4-dihydroxyphenylalanine metabolism to the inhibition of gluconeogenesis in rabbit kidney-cortex tubules
    Jakub Drozak
    Department of Metabolic Regulation, Institute of Biochemistry, Warsaw University, ul Miecznikowa 1, 02 096 Warszawa, Poland
    Int J Biochem Cell Biol 37:1269-80. 2005
    ..is accompanied by 25-40% decrease in glucose production from pyruvate, alanine + glycerol + octanoate and dihydroxyacetone due to augmented generation of hydrogen peroxide via monoamine oxidase B, resulting in a decline of ..
  28. ncbi Dihydroxyacetone and methylglyoxal as permeants of the Plasmodium aquaglyceroporin inhibit parasite proliferation
    Slavica Pavlovic-Djuranovic
    Department of Pharmaceutical Biochemistry, University of Tubingen, Morgenstelle 8, D 72076 Tubingen, Germany
    Biochim Biophys Acta 1758:1012-7. 2006
    ..we show PfAQP permeability for the glycolysis-related metabolites methylglyoxal, a cytotoxic byproduct, and dihydroxyacetone, a ketotriose. AQP3, the red cell aquaglyceroporin, also passed dihydroxacetone but excluded methylglyoxal...
  29. ncbi Production of Gluconobacter oxydans cells from low-cost culture medium for conversion of glycerol to dihydroxyacetone
    Shenghua Wei
    State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China
    Prep Biochem Biotechnol 37:113-21. 2007
    Gluconobacter oxydans could be immobilized as a biocatalyst for the conversion of glycerol to dihydroxyacetone. To reduce the production cost, the cells were produced from agricultural byproducts...
  30. ncbi Repeated use of immobilized Gluconobacter oxydans cells for conversion of glycerol to dihydroxyacetone
    Shenghua Wei
    State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, PR China
    Prep Biochem Biotechnol 37:67-76. 2007
    b>Dihydroxyacetone (DHA) is of great interest in the fine chemical and pharmaceutical industry; therefore, the discovery of suitable biocatalysts for the efficient production of it is very necessary...
  31. ncbi The effect of sunless tanning on behavior in the sun: a pilot study
    Daniel J Sheehan
    Section of Dermatology, Department of Medicine, Medical College of Georgia, Augusta 30904, USA
    South Med J 98:1192-5. 2005
    ..quot;Sunless" tanning methods that avoid UVL exposure may represent a safe alternative. However, the effects of sunless tanning methods on ultraviolet light-related behaviors have never been investigated...
  32. ncbi Factors influencing sunless tanning with dihydroxyacetone
    B C Nguyen
    Wellman Laboratories of Photomedicine, Department of Dermatology, Massachusetts General Hospital, WEL 224, Harvard Medical School, 50 Blossom Street, Boston, MA 02114, USA
    Br J Dermatol 149:332-40. 2003
    ..The pigment is the product of reactions between dihydroxyacetone (DHA) and amino acids in the stratum corneum.
  33. ncbi Durability of the sun protection factor provided by dihydroxyacetone
    A Faurschou
    Department of Dermatology, Bispebjerg Hospital, Bispebjerg Bakke, University of Copenhagen, 23 DK 2400 Copenhagen NV, Denmark
    Photodermatol Photoimmunol Photomed 20:239-42. 2004
    The sunless tanning agent dihydroxyacetone (DHA) is known to protect against longwave ultraviolet radiation (UVA) and visible light...
  34. ncbi Sunless skin tanning with dihydroxyacetone delays broad-spectrum ultraviolet photocarcinogenesis in hairless mice
    Anita B Petersen
    Department of Dermatology D92, Bispebjerg Hospital, Bispebjerg Bakke 23, DK 2400, Copenhagen NV, Denmark
    Mutat Res 542:129-38. 2003
    Sunless tanning with dihydroxyacetone (DHA) is not considered to be a sunscreen although it does absorb parts of the ultraviolet (UV) spectrum...
  35. ncbi Don't be in the dark about tanning
    Michelle Meadows
    FDA Consum 37:16-7. 2003
  36. ncbi By the way, doctor. I'd like to keep the tanned look I got during summer vacation. Are self-tanning lotions and sprays a good idea? Are they safe to use?
    Celeste Robb-Nicholson
    Harv Womens Health Watch 14:8. 2006
  37. ncbi The maillard reaction for sunlight protection
    Ramon M Fusaro
    Dept of Internal Medicine, 984360 Nebraska Medical Center, Omaha, NE 68198 4360, USA
    Ann N Y Acad Sci 1043:174-83. 2005
    ..spring, summer, and fall, 30 UVA/B/Soret band-photosensitive patients used sequential topical applications of dihydroxyacetone (DHA) followed by naphthoquinone only at bedtime and received excellent photoprotection without a single ..
  38. ncbi Cloning, heterologous expression, and characterization of three aquaglyceroporins from Trypanosoma brucei
    Nestor L Uzcategui
    Biochemical Institute, Department of Pharmaceutical Biochemistry, and Institute of Physiology, University of Tubingen, 72076 Tübingen Germany
    J Biol Chem 279:42669-76. 2004
    ..TbAQP expression in Xenopus oocytes increased permeability for water, glycerol and, interestingly, dihydroxyacetone. Except for urea, TbAQPs were virtually impermeable for other polyols; only TbAQP3 transported erythritol and ..
  39. ncbi Comparative genomic analysis of dha regulon and related genes for anaerobic glycerol metabolism in bacteria
    Jibin Sun
    GBF German Research Center for Biotechnology, Mascheroder Weg 1, 38124 Braunschweig, Germany
    Biotechnol Prog 19:263-72. 2003
    The dihydroxyacetone (dha) regulon of bacteria encodes genes for the anaerobic metabolism of glycerol...
  40. ncbi Cloning, sequencing and characterization of a gene encoding dihydroxyacetone kinase from Zygosaccharomyces rouxii NRRL2547
    Zheng Xiang Wang
    Department of Microbiology, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa
    Yeast 19:1447-58. 2002
    The dihydroxyacetone pathway, an alternative pathway for the dissimilation of glycerol via reduction by glycerol dehydrogenase and subsequent phosphorylation by dihydroxyacetone (DHA) kinase, is activated in the yeasts Saccharomyces ..
  41. ncbi Cloning and overexpression in Escherichia coli of the gene encoding dihydroxyacetone kinase isoenzyme I from Schizosaccharomyces pombe, and its application to dihydroxyacetone phosphate production
    N Itoh
    Biotechnology Research Center, Toyama Prefectural University, Japan
    Appl Microbiol Biotechnol 51:193-200. 1999
    The gene dak1 encoding a dihydroxyacetone kinase (DHAK) isoenzyme I, one of two isoenzymes in the Schizosaccharomyces pombe IFO 0354 strain, was cloned and sequenced. The dak1 gene comprises 1743 bp and encodes a protein of 62,245 Da...
  42. ncbi A metabolic study of the regulation of proteolysis by sugars in maize root tips: effects of glycerol and dihydroxyacetone
    Renaud Brouquisse
    Unité Mixte de Recherche de Physiologie et Biotechnologie Végétales, Institut National de la Recherche Agronomique, Universite de Bordeaux 1, IFR 103, BP 81, 33883 Villenave d Ornon Cedex, France
    Planta 225:693-709. 2007
    ..sugar sensing and signaling we compared the effects of carbon starvation with those of glucose, glycerol and dihydroxyacetone on carbon metabolism, proteolysis, and protease expression in excised maize (Zea mays L.) root tips...
  43. ncbi Glyceraldehyde metabolism in human erythrocytes in comparison with that of glucose and dihydroxyacetone
    Tadao Taguchi
    Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University, Nagoya 468 8503, Japan
    Cell Biochem Funct 20:223-6. 2002
    Metabolism of D-glyceraldehyde in human erythrocytes in comparison with that of glucose and dihydroxyacetone was studied. Both trioses were metabolized to produce L-lactate at rates comparable to that of L-lactate formation from glucose...
  44. ncbi Mitochondrial metabolism sets the maximal limit of fuel-stimulated insulin secretion in a model pancreatic beta cell: a survey of four fuel secretagogues
    Peter A Antinozzi
    Division of Clinical Biochemistry and Experimental Diabetology, Department of Internal Medicine, University Medical Center, CH 1211 Geneva 4, Switzerland
    J Biol Chem 277:11746-55. 2002
    ..each of these metabolic pathways, we have compared the fates of four fuel secretagogues (glucose, pyruvate, dihydroxyacetone, and glycerol) in the INS1-E beta cell line...
  45. doi Interrelation between the inhibition of glycolytic flux by silibinin and the lowering of mitochondrial ROS production in perifused rat hepatocytes
    Dominique Detaille
    Departamento de Fisiologia y Farmacologia, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno, E D S 11, E 37007 Salamanca, Spain
    Life Sci 82:1070-6. 2008
    ....
  46. ncbi Mimicking fructose and rhamnulose aldolases: organocatalytic syn-aldol reactions with unprotected dihydroxyacetone
    S S V Ramasastry
    The Skaggs Institute for Chemical Biology and Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Angew Chem Int Ed Engl 46:5572-5. 2007
  47. ncbi Mimicking aldolases through organocatalysis: syn-selective aldol reactions with protected dihydroxyacetone
    Naoto Utsumi
    The Skaggs Institute for Chemical Biology and Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Org Lett 9:3445-8. 2007
    ..Threonine-based catalysts facilitated the aldol reaction of protected dihydroxyacetone or protected hydroxacetone with a variety of aldehydes to provide syn-aldol products with good yields and ee'..
  48. doi Highly enantioselective direct syn- and anti-aldol reactions of dihydroxyacetones catalyzed by chiral primary amine catalysts
    Sanzhong Luo
    Beijing National Laboratory for Molecular Sciences BNLMS, Center for Chemical Biology, Institute of Chemistry and Graduate School, Chinese Academy of Sciences, Beijing 100080, China
    Org Lett 10:653-6. 2008
    ..This type of organocatalysts functionally mimics all four DHA aldolases, namely L-fuculose-1-phosphate aldolase, D-tagatose-1,6-diphosphate aldolase, D-fructose-1,6-diphosphate aldolase, and L-rhamnulose-1-phosphate aldolase...
  49. ncbi [Effects of H2O2 addition on oxygen supply and metabolism of microorganisms]
    Shuliang Li
    Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, Beijing 100084, China
    Wei Sheng Wu Xue Bao 42:129-32. 2002
  50. ncbi Influence of hydration on dihydroxyacetone-induced pigmentation of stratum corneum
    Bach Cuc Nguyen
    Wellman Laboratories of Photomedicine, Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA
    J Invest Dermatol 120:655-61. 2003
    b>Dihydroxyacetone, the browning ingredient in sunless tanning formulations, reacts with amino acids in the outer stratum corneum to form a mixture of high molecular weight pigments...
  51. ncbi Modulated photoacoustic spectroscopy study of an artificial tanning on human skin induced by dihydroxyacetone
    N Benamar
    Departement de Physique, Faculté des Sciences de Meknès, Université Moulay Ismaïl BP 4010 Beni M hamed, Meknes, Morocco
    Physiol Meas 25:1199-210. 2004
    A modulated photoacoustic spectroscopy study on the effect of dihydroxyacetone, commonly used for artificial tan, is presented...
  52. ncbi Sunless tanning
    Jennifer M Fu
    Memorial Sloan Kettering Cancer Center, Dermatology Service, Department of Medicine, New York, New York 10022, USA
    J Am Acad Dermatol 50:706-13. 2004
    ..Recent data indicate continued high incidence of sunburn and ultraviolet tanning despite public health skin cancer prevention messages. The rising popularity of recently available sunless tanning booths prompted this review...
  53. ncbi Sun protection effect of dihydroxyacetone
    Annesofie Faurschou
    Arch Dermatol 140:886-7. 2004
  54. ncbi UV-generated free radicals (FR) in skin: their prevention by sunscreens and their induction by self-tanning agents
    K Jung
    Gematria Test Lab, Berlin, Germany
    Spectrochim Acta A Mol Biomol Spectrosc 69:1423-8. 2008
    ..effect of UV filters and sunscreens as well as the radical induction capacity of self-tanning agents as dihydroxyacetone (DHA)...
  55. ncbi Xanthotrichia (yellow hair) due to selenium sulfide and dihydroxyacetone
    Noel Prevost
    University of Pittsburgh, Department of Dermatology, Pittsburgh, PA 15213, USA
    J Drugs Dermatol 7:689-91. 2008
    ..Two cases of new chemicals causing yellow hair shaft discoloration are reported. The chemicals include selenium sulfide 2.5% shampoo and dihydroxyacetone.
  56. ncbi Self-tanning lotions: are they a healthy way to achieve a tan?
    Zoe D Draelos
    Department of Dermatology, Wake Forest University, North Carolina and Dermatology Consulting Services, Winston Salem, High Point, North Carolina, USA
    Am J Clin Dermatol 3:317-8. 2002
    Self-tanning creams utilize dihydroxyacetone (DHA) as an active agent, to produce a temporary staining of the skin...
  57. ncbi Dihydroxyacetone, the active browning ingredient in sunless tanning lotions, induces DNA damage, cell-cycle block and apoptosis in cultured HaCaT keratinocytes
    Anita B Petersen
    Department of Dermatology, Bispebjerg Hospital, Bispebjerg Bakke 23, DK 2400 Copenhagen, NV, Denmark
    Mutat Res 560:173-86. 2004
    b>Dihydroxyacetone (DHA), the active substance in sunless tanning lotions reacts with the amino groups of proteins to form a brown-colored complex...
  58. ncbi Detailed investigation of the production of the bread flavor component 6-acetyl-1,2,3,4-tetrahydropyridine in proline/1,3-dihydroxyacetone model systems
    An Adams
    Department of Organic Chemistry, Faculty of Agricultural and Applied Biological Sciences, Ghent University, Coupure Links 653, B 9000 Ghent, Belgium
    J Agric Food Chem 52:5685-93. 2004
    ..ATHP), an important Maillard flavor component, in the reaction of L-(-)-proline and 1,3-dihydroxyacetone was investigated as a function of different reaction conditions...
  59. ncbi Is reduction of the sulfonated tetrazolium 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2-tetrazolium 5-carboxanilide a reliable measure of intracellular superoxide production?
    Ludmil Benov
    The Department of Biochemistry, Faculty of Medicine, Kuwait University, P O Box 24923, 13110, Safat, Kuwait
    Anal Biochem 310:186-90. 2002
    ..We now show that soluble extracts of Escherichia coli contain two NADPH:XTT reductases that act aerobically or anaerobically. That being the case, XTT reduction is not a reliable measure of intracellular O(2)(-)...
  60. ncbi The nicotinamide biosynthetic pathway is a by-product of the RNA world
    H J Cleaves
    Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0506, USA
    J Mol Evol 52:73-7. 2001
    ..The most primitive pathway for nicotinic acid biosynthesis is the reaction of aspartic acid with dihydroxyacetone phosphate...
  61. ncbi Superoxide-dependence of the short chain sugars-induced mutagenesis
    Ludmil Benov
    The Department of Biochemistry, Faculty of Medicine, Kuwait University, Safat, Kuwait
    Free Radic Biol Med 34:429-33. 2003
    ..Glycolaldehyde, glyceraldehyde, and dihydroxyacetone greatly enhanced the mutation rates in SOD-deficient E. coli...
  62. ncbi H2S cytotoxicity mechanism involves reactive oxygen species formation and mitochondrial depolarisation
    Mohammad A Eghbal
    Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Toronto, Ontario, Canada M5S 2S2
    Toxicology 203:69-76. 2004
    ..Treatment of H2S poisoning may benefit from interventions aimed at minimizing ROS-induced damage and reducing mitochondrial damage...
  63. ncbi Overexpression of short heterodimer partner recovers impaired glucose-stimulated insulin secretion of pancreatic beta-cells overexpressing UCP2
    Y H Suh
    Department of Physiology and Chronic Disease Research Center, Keimyung University School of Medicine, 194 Dongsan dong, Jung Gu, Daegu, 700 712, South Korea
    J Endocrinol 183:133-44. 2004
    ..KATP channel inhibition mediated by dihydroxyacetone, which gives reducing equivalents directly to complex II of the electron transport system, was similar in Ad-..
  64. ncbi Glucose 6-phosphate hydrolysis is activated by glucagon in a low temperature-sensitive manner
    C Ichai
    , , Grenoble 38041, France
    J Biol Chem 276:28126-33. 2001
    ..By using a model of liver cells, perifused with dihydroxyacetone, we show here that the acute stimulation of gluconeogenesis by glucagon (10(-7) m) was not related to the ..
  65. ncbi Crystal structures of human glycerol 3-phosphate dehydrogenase 1 (GPD1)
    Xianjin Ou
    National Laboratory of Biomacromolecules, Institute of Biophysics IBP, Chinese Academy of Sciences, Beijing 100101, China
    J Mol Biol 357:858-69. 2006
    ..sapiens L-alpha-glycerol-3-phosphate dehydrogenase 1 (GPD1) catalyzes the reversible biological conversion of dihydroxyacetone (DHAP) to glycerol-3-phosphate...
  66. ncbi The dihydroxyacetone unit--a versatile C(3) building block in organic synthesis
    Dieter Enders
    Institut fur Organische Chemie, Rheinisch Westfalische Technische Hochschule, Professor Pirlet Strasse 1, 52074 Aachen, Germany
    Angew Chem Int Ed Engl 44:1304-25. 2005
    Nature employs dihydroxyacetone phosphate (DHAP) as the donor component in various enzyme-catalyzed aldol reactions...
  67. ncbi Microbial aldolases as C-C bonding enzymes--unknown treasures and new developments
    Anne K Samland
    Institut fur Mikrobiologie, Universitat Stuttgart, Germany
    Appl Microbiol Biotechnol 71:253-64. 2006
    ..These are either other dihydroxyacetone phosphate aldolases or aldolases depending on pyruvate/phosphoenolpyruvate, glycine, or acetaldehyde as donor ..
  68. ncbi Dihydroxyacetone variants in the organocatalytic construction of carbohydrates: mimicking tagatose and fuculose aldolases
    Jeff T Suri
    Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    J Org Chem 71:3822-8. 2006
    b>Dihydroxyacetone variants have been explored as donors in organocatalytic aldol reactions with various aldehyde and ketone acceptors...
  69. ncbi Subcellular localization of liver FBPase is modulated by metabolic conditions
    Alejandro J Yañez
    Instituto de Bioquimica, Facultad de Ciencias, Universidad Austral de Chile, Casilla 567, Valdivia, Chile
    FEBS Lett 577:154-8. 2004
    In primary cultured hepatocytes, fructose-1,6-bisphosphatase (FBPase) localization is modulated by glucose, dihydroxyacetone (DHA) and insulin...
  70. pmc Crystal structure of the reduced Schiff-base intermediate complex of transaldolase B from Escherichia coli: mechanistic implications for class I aldolases
    J Jia
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Protein Sci 6:119-24. 1997
    Transaldolase catalyzes transfer of a dihydroxyacetone moiety from a ketose donor to an aldose acceptor...
  71. ncbi A convenient synthesis of immunosuppressive agent FTY720 using the petasis reaction
    Shigeo Sugiyama
    Meiji Pharmaceutical University, Tokyo, Japan
    Chem Pharm Bull (Tokyo) 53:100-2. 2005
    ..Hydroboration of 11 using catecholborane and hydrolysis gave (E)-2-(4-octylphenyl)vinylboronic acid (4). The Petasis reaction of 4, dihydroxyacetone (3), and benzylamine following catalytic hydrogenation afforded FTY720 (1).
  72. ncbi New quinoproteins in oxidative fermentation
    O Adachi
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753 8515, Japan
    Biochim Biophys Acta 1647:10-7. 2003
    ..b>Dihydroxyacetone can be replaced by L-erythrulose for cosmetics for those who are sensitive to dihydroxyacetone...
  73. ncbi New developments in oxidative fermentation
    O Adachi
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753 8515, Japan
    Appl Microbiol Biotechnol 60:643-53. 2003
    ..oxidize meso-erythritol at 37 degrees C leading to the accumulation of L-erythrulose, which may replace dihydroxyacetone in cosmetics. G...
  74. ncbi Pseudo infantile Refsum's disease: catalase-deficient peroxisomal particles with partial deficiency of plasmalogen synthesis and oxidation of fatty acids
    P Aubourg
    INSERM U342, Hopital Saint Vincent de Paul, Paris, France
    Pediatr Res 34:270-6. 1993
    ..of phytanic and pipecolic acids was severely impaired, whereas oxidation of very-long-chain fatty acids and dihydroxyacetone phosphate acyltransferase activity were only partially decreased...
  75. pmc Starch degradation in chloroplasts isolated from C3 or CAM (crassulacean acid metabolism)-induced Mesembryanthemum crystallinum L
    H E Neuhaus
    Fachbereich Biologie chemie, Universitat Osnabruck, Germany
    Biochem J 318:945-53. 1996
    ..exported into the incubation medium by these chloroplasts are glucose 6-phosphate, 3-phosphoglyceric acid, dihydroxyacetone phosphate and glucose...
  76. ncbi Immunological analyses of alkyl-dihydroxyacetone-phosphate synthase in human peroxisomal disorders
    J Biermann
    Centre for Biomembranes and Lipid Enzymology, Institute for Biomembranes, Utrecht University, The Netherlands
    Eur J Cell Biol 78:339-48. 1999
    ..Immunoelectron microscopy showed that the enzyme is predominantly located on the lumenal side of the peroxisomal membrane in rat and guinea pig liver...
  77. pmc Structural insights into the substrate binding and stereoselectivity of giardia fructose-1,6-bisphosphate aldolase
    Andrey Galkin
    W M Keck Laboratory for Structural Biology, Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Biochemistry 48:3186-96. 2009
    ..class II zinc-dependent aldolase family that catalyzes the cleavage of d-fructose 1,6-bisphosphate (FBP) into dihydroxyacetone phosphate (DHAP) and d-glyceraldehyde 3-phosphate (G3P)...
  78. ncbi In vitro and in vivo evaluation of various carbonyl compounds against cyanide toxicity with particular reference to alpha-ketoglutaric acid
    Rahul Bhattacharya
    Defence Research and Development Establishment, Division of Pharmacology and Toxicology, Gwalior, India
    Drug Chem Toxicol 31:149-61. 2008
    ..acid, maleic acid, malic acid, fumaric and oxaloacetic acid, glucose, sucrose, fructose, mannitol, sorbitol, dihydroxyacetone, and glyoxal (5 or 10 mM; -10 min) against toxicity of potassium cyanide (KCN; 10 mM) in rat thymocytes in ..
  79. doi Betaines and dimethylsulfoniopropionate as major osmolytes in cnidaria with endosymbiotic dinoflagellates
    Paul H Yancey
    Biology Department, Whitman College, Walla Walla, Washington 99362, USA
    Physiol Biochem Zool 83:167-73. 2010
    ..An enzymatic assay for glycerol plus glycerol 3-phosphate and dihydroxyacetone phosphate yielded 1-10 mmol/kg...
  80. ncbi Dihydroxyacetone phosphate acyltransferase and alkyldihydroxyacetone phosphate synthase activities in rat liver subcellular fractions and human skin fibroblasts
    H Singh
    Department of Chemical Pathology, Adelaide Children s Hospital, South Australia
    Arch Biochem Biophys 268:676-86. 1989
    b>Dihydroxyacetone phosphate acyltransferase (DHAP-AT) and alkyldihydroxyacetone phosphate synthase (DHAP-synthase) activities were examined in subcellular fractions of rat liver...
  81. doi Proteotoxicity and the contrasting effects of oxaloacetate and glycerol on Caenorhabditis elegans life span: a role for methylglyoxal?
    Alan R Hipkiss
    School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, The University of Birmingham, Edgbaston, Birmingham, United Kingdom
    Rejuvenation Res 13:547-51. 2010
    ..It is proposed that, if not secreted by aquaporin, glycerol is converted to glycerol phosphate and then to dihydroxyacetone phosphate (DHAP) via a reaction requiring nicotinamide adenine dinucleotide (NAD(+))...
  82. pmc Glycerol is metabolized in a complex and strain-dependent manner in Enterococcus faecalis
    Alain Bizzini
    Laboratoire de Microbiologie de l Environnement, Universite de Caen, EA956 USC INRA2017 IFR 146, 14032 Caen Cedex, France
    J Bacteriol 192:779-85. 2010
    ..oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone kinase (the dhaK pathway)...
  83. pmc Transaldolase B of Escherichia coli K-12: cloning of its gene, talB, and characterization of the enzyme from recombinant strains
    G A Sprenger
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Julich, Germany
    J Bacteriol 177:5930-6. 1995
    ..gene onto high-copy-number vectors, transaldolase B (D-sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphate dihydroxyacetone transferase; EC 2.2.1.2) was overexpressed up to 12.7 U mg of protein-1 compared with less than 0...
  84. ncbi Metabolic flux and metabolic network analysis of Penicillium chrysogenum using 2D [13C, 1H] COSY NMR measurements and cumulative bondomer simulation
    Wouter A van Winden
    Bioprocestechnology Group, Faculty of Applied Sciences, Delft University of Technology, The Netherlands
    Biotechnol Bioeng 83:75-92. 2003
    ..transketolase and transaldolase reactions, extending the glycolysis with a fructose 6-phosphate aldolase/dihydroxyacetone kinase reaction sequence or adding a phosphoenolpyruvate carboxykinase reaction to the model considerably ..
  85. pmc Microbial conversion of glycerol to 1,3-propanediol: physiological comparison of a natural producer, Clostridium butyricum VPI 3266, and an engineered strain, Clostridium acetobutylicum DG1(pSPD5)
    Maria Gonzalez-Pajuelo
    Escola Superior B8iotecnologia, Universidade Catolica Portuguesa, Rua Dr Antonio Bernardino de Almeida, 4200 072 Porto, Portugal
    Appl Environ Microbiol 72:96-101. 2006
    ..Firstly, the pathway for glycerol oxidation was different: C. butyricum uses a glycerol dehydrogenase and a dihydroxyacetone kinase, while C. acetobutylicum uses a glycerol kinase and a glycerol-3-phosphate dehydrogenase...
  86. ncbi Dihydroxyacetone phosphate aldolase catalyzed synthesis of structurally diverse polyhydroxylated pyrrolidine derivatives and evaluation of their glycosidase inhibitory properties
    Jordi Calveras
    Biotransformation and Bioactive Molecules group, Catalonia Institute for Advanced Chemistry CSIC, Jordi Girona 18 26, 08034 Barcelona, Spain
    Chemistry 15:7310-28. 2009
    ..The chemoenzymatic synthesis of a collection of pyrrolidine-type iminosugars generated by the aldol addition of dihydroxyacetone phosphate (DHAP) to C-alpha-substituted N-Cbz-2-aminoaldehydes derivatives, catalyzed by DHAP aldolases is ..
  87. pmc In vivo selection for the directed evolution of L-rhamnulose aldolase from L-rhamnulose-1-phosphate aldolase (RhaD)
    Masakazu Sugiyama
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Bioorg Med Chem 15:5905-11. 2007
    b>Dihydroxyacetone phosphate (DHAP)-dependent aldolases have been widely used for organic synthesis. The major drawback of DHAP-dependent aldolases is their strict donor substrate specificity toward DHAP, which is expensive and unstable...
  88. pmc Rickettsia prowazekii uses an sn-glycerol-3-phosphate dehydrogenase and a novel dihydroxyacetone phosphate transport system to supply triose phosphate for phospholipid biosynthesis
    Kyla M Frohlich
    Laboratory of Molecular Biology, Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, Alabama 36688, USA
    J Bacteriol 192:4281-8. 2010
    ..Here we describe a novel biochemical pathway that relies on rickettsial transport of host cytosolic dihydroxyacetone phosphate (DHAP) and its subsequent conversion to sn-glycerol-3-phosphate (G3P) for synthesis of ..
  89. ncbi Kinetic regulation of the mitochondrial glycerol-3-phosphate dehydrogenase by the external NADH dehydrogenase in Saccharomyces cerevisiae
    Inga Lill Pahlman
    Department of Cellular and Molecular Biology Microbiology, Lundberg Laboratory, Gothenburg University, Sweden
    J Biol Chem 277:27991-5. 2002
    ..In the latter system, NADH is oxidized to NAD+ and dihydroxyacetone phosphate is reduced to glycerol 3-phosphate by the cytosolic Gpd1p; glycerol 3-phosphate gives two electrons ..
  90. pmc Characterization of quinolinate synthases from Escherichia coli, Mycobacterium tuberculosis, and Pyrococcus horikoshii indicates that [4Fe-4S] clusters are common cofactors throughout this class of enzymes
    Allison H Saunders
    Department of Chemistry and Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 47:10999-1012. 2008
    Quinolinate synthase (NadA) catalyzes a unique condensation reaction between iminoaspartate and dihydroxyacetone phosphate, affording quinolinic acid, a central intermediate in the biosynthesis of nicotinamide adenine dinucleotide (NAD)...
  91. pmc Glycerol metabolism is important for cytotoxicity of Mycoplasma pneumoniae
    Claudine Hames
    Department of General Microbiology, Georg August University Gottingen, Grisebachstr 8, 37077 Gottingen, Germany
    J Bacteriol 191:747-53. 2009
    ..involves uptake by facilitated diffusion, phosphorylation, and the oxidation of glycerol 3-phosphate to dihydroxyacetone phosphate, a glycolytic intermediate...
  92. pmc Glycerol metabolism and PrfA activity in Listeria monocytogenes
    Biju Joseph
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Gebäude E1, 97080 Wurzburg, Germany
    J Bacteriol 190:5412-30. 2008
    ..were higher in the presence of glycerol than in the presence of glucose and cellobiose included those for two dihydroxyacetone (Dha) kinases and many genes that are under carbon catabolite repression control...
  93. ncbi Mouse lacking NAD+-linked glycerol phosphate dehydrogenase has normal pancreatic beta cell function but abnormal metabolite pattern in skeletal muscle
    M J MacDonald
    University of Wisconsin Childrens Diabetes Center, Madison 53706, USA
    Arch Biochem Biophys 384:143-53. 2000
    ..Levels of glycerol phosphate (low) and dihydroxyacetone phosphate (high) were very abnormal in nonislet tissue, especially in skeletal muscle...
  94. ncbi Mechanism of the Schiff base forming fructose-1,6-bisphosphate aldolase: structural analysis of reaction intermediates
    Esben Lorentzen
    European Molecular Biology Laboratory, Hamburg Outstation, Germany
    Biochemistry 44:4222-9. 2005
    ..FBPA) catalyzes the reversible cleavage of fructose 1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate...
  95. pmc Identification of arginine 331 as an important active site residue in the class II fructose-1,6-bisphosphate aldolase of Escherichia coli
    S Qamar
    Department of Biochemistry, University of Cambridge, United Kingdom
    Protein Sci 5:154-61. 1996
    ..Comparatively small differences in the inhibitor constant Ki for dihydroxyacetone phosphate or its analogue, 2-phosphoglycolate, were found between the wild-type and mutant enzymes...
  96. ncbi Fructose-6-phosphate aldolase is a novel class I aldolase from Escherichia coli and is related to a novel group of bacterial transaldolases
    M Schurmann
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, D 52425 Julich, Germany
    J Biol Chem 276:11055-61. 2001
    ..mg(-)1 of protein was found; K(m) values for the substrates were 9 mm for fructose 6-phosphate, 35 mm for dihydroxyacetone, and 0.8 mm for glyceraldehyde 3-phosphate...
  97. ncbi Crystallization and preliminary X-ray analysis of native and selenomethionine fructose-1,6-bisphosphate aldolase from Thermus aquaticus
    V Sauvé
    Departement de Biochimie, Universite de Montreal, C P 6128, Succursale Centre Ville, Montreal H3C 3J7, Canada
    Acta Crystallogr D Biol Crystallogr 57:310-3. 2001
    ..C. 4.1.2) catalyses the reversible cleavage of fructose-1,6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde-3-phosphate in the glycolytic pathway of prokaryote and eukaryote organisms...
  98. ncbi Skin pigmentation enhancers
    D A Brown
    AGI Dermatics, 205 Buffalo Avenue, Freeport, NY 11520, USA
    J Photochem Photobiol B 63:148-61. 2001
    ..These include topically applied substances that simulate natural pigmentation: dihydroxyacetone and melanins; and substances that stimulate the natural pigmentation process: psoralens with UVA (PUVA), ..
  99. ncbi Topography of ether phospholipid biosynthesis
    D Hardeman
    Centre for Biomembranes and Lipid Enzymology, University of Utrecht, The Netherlands
    Biochim Biophys Acta 1006:1-8. 1989
    The enzyme, dihydroxyacetone-phosphate acyltransferase (DHAP-AT) is localized at the inner surface of the peroxisomal membrane but shows no latency. The product, i.e...
  100. ncbi Kinetic study of sn-glycerol-1-phosphate dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix K1
    Jin Suk Han
    National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan
    Eur J Biochem 269:969-76. 2002
    ..The enzyme showed substrate specificity for NAD(P)H-dependent dihydroxyacetone phosphate reduction and NAD(+)-dependent glycerol-1-phosphate (Gro1P) oxidation...
  101. ncbi Modulation of the interaction between aldolase and glycerol-phosphate dehydrogenase by fructose phosphates
    B G Vertessy
    Institute of Enzymology, Hungarian Academy of Sciences, Budapest
    Biochim Biophys Acta 1078:236-42. 1991
    ..Fructose-1-phosphate or dihydroxyacetone phosphate had no effect on the dissociation constant of the aldolase-dehydrogenase complex...

Research Grants71

  1. SYNTHESIS OF PHOSPHATE ESTERS WITH IODINE--III REAGENTS
    GERALD KOSER; Fiscal Year: 1990
    ..An economical synthesis of dihydroxyacetone phosphate from propargyl alcohol is delineated, and a new approach to the pentofuranose-3- and 5-phosphates ..
  2. GLYCEROLIPID METABOLISM IN MICROBODIES (PEROXISOMES)
    AMIYA HAJRA; Fiscal Year: 1990
    ..In our laboratory, we have shown that the key enzymes of the acyl dihydroxyacetone phosphate pathway for glycerolipid biosynthesis are localized in microbodies...
  3. MECHANISMS INVOLVED IN FLAVIN-LINKED OXYGEN METABOLISM
    H CLAIBORNE; Fiscal Year: 2004
    ..from Enterococcus faecalis, and (3) alpha-glycerophosphate oxidase (GlpO; alpha-glycerophosphate + O2 - to dihydroxyacetone phosphate + H2O2) from Streptococcus sp...
  4. MECHANISMS INVOLVED IN FLAVIN-LINKED OXYGEN METABOLISM
    H CLAIBORNE; Fiscal Year: 2003
    ..from Enterococcus faecalis, and (3) alpha-glycerophosphate oxidase (GlpO; alpha-glycerophosphate + O2 - to dihydroxyacetone phosphate + H2O2) from Streptococcus sp...
  5. MECHANISMS INVOLVED IN FLAVIN-LINKED OXYGEN METABOLISM
    H CLAIBORNE; Fiscal Year: 2002
    ..from Enterococcus faecalis, and (3) alpha-glycerophosphate oxidase (GlpO; alpha-glycerophosphate + O2 - to dihydroxyacetone phosphate + H2O2) from Streptococcus sp...
  6. MECHANISMS INVOLVED IN FLAVIN-LINKED OXYGEN METABOLISM
    Al Claiborne; Fiscal Year: 2005
    ..from Enterococcus faecalis, and (3) alpha-glycerophosphate oxidase (GlpO; alpha-glycerophosphate + O2 - to dihydroxyacetone phosphate + H2O2) from Streptococcus sp...
  7. PHOSPHATIDIC ACID & ANALOG METABOLISM IN LIVER & BRAIN
    AMIYA HAJRA; Fiscal Year: 1993
    ..The specific objective of this proposal is to characterize the enzymes of the acyl dihydroxyacetone phosphate (acyl DHAP) pathway which catalyze the biosynthesis of the key membrane lipid precursors, ..
  8. GLYCEROL DISSIMILATION AND ITS REGULATION IN BACTERIA
    EDMUND LIN; Fiscal Year: 1990
    ..In the glp system, sn-glycerol 3-phosphate (C3P) is the central intermediate that is oxidized to dihydroxyacetone phosphate by either of two dehydrogenases of the flavoprotein kind: an aerobic dehydrogenase glpD product) ..
  9. METABOLISM OF PHOSPHATIDIC ACID AND ITS ANALOGS IN LIVER
    AMIYA HAJRA; Fiscal Year: 1990
    ..Detailed studies will be made on the enzymes of the acyl dihydroxyacetone phosphate (acyl DHAP) pathway, namely DHAP acyltransferase, alkyl DHAP synthase and acyl/alkyl DHAP reductase...
  10. Identification of Metabolic Virulence Factors in the Epidemic Typhus Rickettsia
    JONATHON AUDIA; Fiscal Year: 2007
    ..DPC) and 2) rickettsial acquisition of sn-glycerol-3-phosphate (G3P) via transport of G3P and dihydroxyacetone phosphate (DHAP)...
  11. HORMONE AND DIET CONTROL OF GLUCONEOGENESIS
    Paul Ray; Fiscal Year: 1980
    ..We find fasted rabbits to have a distribution of hepatic PEPCK similar to humans. Lactate- and dihydroxyacetone-derived gluconeogenesis in rabbits is enhanced by glucagon, epinephrine and cyclic AMP and regulation of ..
  12. Genetic Dissection of Age-dependent Neuroprotection Mechanisms in Drosophila
    BARRY S GANETZKY; Fiscal Year: 2010
    ..encodes the glycolytic enzyme, triose phosphate isomerase (Tpi) responsible for the interconversion of DHAP (dihydroxyacetone phosphate) and GAP (glyceraldehyde 3-phosphate), only the latter of which is able to continue through ..
  13. Genetic Dissection of Age-dependent Neuroprotection Mechanisms in Drosophila
    Barry Ganetzky; Fiscal Year: 2009
    ..encodes the glycolytic enzyme, triose phosphate isomerase (Tpi) responsible for the interconversion of DHAP (dihydroxyacetone phosphate) and GAP (glyceraldehyde 3-phosphate), only the latter of which is able to continue through ..
  14. CALIXARENES AS ENZYME MODELS
    C Gutsche; Fiscal Year: 1980
    ..attention being given to their ability to catalyze ester and amide hydrolyses (chymotrypsin model), dihydroxyacetone phosphate condensations (aldolase model), farnesol phosphate cyclizations, dihydropyridine-carbondyl hydrogen ..
  15. BIOLOGICALLY IMPORTANT ENOLS
    ALEXANDER KRESGE; Fiscal Year: 1990
    ..For example, further insight would gained into the mechanism of conversion of dihydroxyacetone phosphate into gyceraldehyde-3-phosphate through catalysis by triosephosphate isomerase, which is a critical ..
  16. GLYCEROLIPID METABOLISM IN MICROBODIES
    AMIYA HAJRA; Fiscal Year: 1980
    The major objective of the proposed research is to establish the presence of the enzymes of the acyl dihydroxyacetone phosphate pathway for glycerolipid biosynthesis in the microbodies (peroxisomes) of liver and brain...
  17. PHOSPHOLIPID METABOLISM DURING LUNG DEVELOPMENT
    Paul Weinhold; Fiscal Year: 1980
    ..The pool sizes of choline phosphate, CDP-choline, CTP, CMP, glycerol-3-phosphate, dihydroxyacetone phosphate and lactate are measured in fetal and adult lung...
  18. METABOLISM OF PHOSPHATIDIC ACID & ITS ANALOGS IN BRAIN
    AMIYA HAJRA; Fiscal Year: 1980
    ..of the proposed research is to evaluate the relative contribution of glycerol-3-phosphate pathway and acyl dihydroxyacetone phosphate pathway to the formation of phosphatidic acid in brain and liver...
  19. METABOLISM OF PYRUVATE ESTERS AND THIOESTERS
    Henri Brunengraber; Fiscal Year: 1999
    ..project, the investigators will test the hypothesis that dipruvyl-acetyl glycerol (DPAG), pyruvyl-acetyl- dihydroxyacetone (PADA), and pyruvate N-acetylcysteine ethyl ester (PNACE) can deliver therapeutic levels of pyruvate without ..
  20. METABOLISM OF PYRUVATE ESTERS AND THIOESTERS
    Henri Brunengraber; Fiscal Year: 2000
    ..project, the investigators will test the hypothesis that dipruvyl-acetyl glycerol (DPAG), pyruvyl-acetyl- dihydroxyacetone (PADA), and pyruvate N-acetylcysteine ethyl ester (PNACE) can deliver therapeutic levels of pyruvate without ..
  21. CONTROL PROCESSES IN MAMMALIAN CELLS
    Henry Lardy; Fiscal Year: 1991
    ..task is to establish a pathway by which reducing equivalents generated by malic enzyme can be used to convert dihydroxyacetone phosphate to sn-glycerol 3-phosphate...
  22. OXYGEN DELIVERY AND METABOLISM IN MYOCARDIAL ISCHEMIA
    George Pantely; Fiscal Year: 1980
    The affinity of blood for oxygen can be decreased acutely by the infusion of a solution of dihydroxyacetone, phosphate, and pyruvate or ortho-iodo sodium benzoate...
  23. ETHER LIPIDS IN CANCER-ENZYME MECHANISMS
    FRED SNYDER; Fiscal Year: 1980
    ..the unique reaction mechanism that substitutes a long chain fatty alcohol in toto for the acyl group of acyl dihydroxyacetone-P to form the alkyl ether linkage...
  24. Investigation of a Behavioral Substitute for Sunbathing
    Sherry Pagoto; Fiscal Year: 2006
    ..products that come in the form of creams, foams, and sprays to be applied directly to the skin and contain dihydroxyacetone (DMA), a colorless vegetable-derived sugar that temporarily stains the skin a tan color...
  25. Investigation of a Behavioral Substitute for Sunbathing
    Sherry Pagoto; Fiscal Year: 2007
    ..products that come in the form of creams, foams, and sprays to be applied directly to the skin and contain dihydroxyacetone (DMA), a colorless vegetable-derived sugar that temporarily stains the skin a tan color...
  26. AN ENZYME MECHANISTIC PATHWAY AT ATOMIC RESOLUTION
    Gregory Petsko; Fiscal Year: 1980
    ..We plan to add the substrate dihydroxyacetone phosphate to the crystals at minus 10 degrees C and collect a complete set of high resolution diffraction ..
  27. O-ALKYL LIPIDS IN SURFACE MEMBRANES OF TUMOR CELLS
    SAMUEL FRIEDBERG; Fiscal Year: 1980
    ..provided evidence to show that the O-alkyl lipid bond is formed via the intermediacy of an endiol of acyl dihydroxyacetone-P...
  28. TOTAL SYNTHESIS OF KINAMYCINS AND LOMAIVITICINS
    K Nicolaou; Fiscal Year: 2007
    ..feature a unified, highly convergent strategy involving the sequential asymmetric alkylation of a chiral 1,3-dihydroxyacetone equivalent to establish the complete carbon framework of the target molecules, followed by a highly selective ..
  29. SYNTHESIS OF ANTICANCER AGENTS
    K C Nicolaou; Fiscal Year: 2010
    ..feature a unified, highly convergent strategy involving the sequential asymmetric alkylation of a chiral 1,3-dihydroxyacetone equivalent to establish the complete carbon framework of the target molecules, followed by a highly selective ..
  30. SYNTHESIS OF ANTICANCER AGENTS
    K Nicolaou; Fiscal Year: 2006
    ..feature a unified, highly convergent strategy involving the sequential asymmetric alkylation of a chiral 1,3-dihydroxyacetone equivalent to establish the complete carbon framework of the target molecules, followed by a highly selective ..
  31. SYNTHESIS OF ANTICANCER AGENTS
    K Nicolaou; Fiscal Year: 2009
    ..feature a unified, highly convergent strategy involving the sequential asymmetric alkylation of a chiral 1,3-dihydroxyacetone equivalent to establish the complete carbon framework of the target molecules, followed by a highly selective ..
  32. REACTIVE INTERMEDIATES OF ENZYMATIC REACTIONS
    John Richard; Fiscal Year: 2009
    ..The focus of this application is the critical role of flexible phosphate gripper loops in enzymatic catalysis and the results may spur efforts to develop novel enzyme inhibitors that specifically target these loops. ..
  33. REACTIVE INTERMEDIATES OF ENZYMATIC REACTIONS
    John Richard; Fiscal Year: 1999
    ....
  34. REACTIVE INTERMEDIATES OF ENZYMATIC REACTIONS
    John Richard; Fiscal Year: 2006
    ..abstract_text> ..
  35. Ribozymes for new genetic coding systems
    John Richard; Fiscal Year: 2006
    ..abstract_text> ..
  36. REACTIVE INTERMEDIATES OF ENZYMATIC REACTIONS
    John Richard; Fiscal Year: 2005
    ..abstract_text> ..
  37. Ribozymes for new genetic coding systems
    John Richard; Fiscal Year: 2005
    ..abstract_text> ..
  38. REACTIVE INTERMEDIATES OF ENZYMATIC REACTIONS
    John Richard; Fiscal Year: 2003
    ....
  39. REACTIVE INTERMEDIATES OF ENZYMATIC REACTIONS
    John Richard; Fiscal Year: 2004
    ....
  40. Structural Studies of Metabolic Membrane Proteins
    Joanne Yeh; Fiscal Year: 2004
    ..Our specific aims are to obtain atomic resolution structures of both membrane proteins, both of which are likely to be novel targets for antibiotic design and therapy. ..
  41. Glucose/Secreatogogue Metabolism in Pancreatic islets
    Michael MacDonald; Fiscal Year: 2007
    ..Resulting decreases in insulin release will be compared with alterations in metabolic pathways. In Aim 2B limited focused studies of beta ceil cataplerosis in type 2 diabetes will be performed. ..
  42. Childhood Diabetes Clinical & Molecular Research Training Program
    Michael MacDonald; Fiscal Year: 2008
    ..Given these opportunities, the CDCMRT program can provide trainees with a comprehensive mentored research training experience that will prepare them for successful research careers in childhood diabetes, insulin resistance and obesity. ..
  43. Structural Studies of Metabolic Membrane Proteins
    Joanne Yeh; Fiscal Year: 2003
    ..Our specific aims are to obtain atomic resolution structures of both membrane proteins, both of which are likely to be novel targets for antibiotic design and therapy. ..
  44. Structural Studies of Metabolic Membrane Proteins
    Joanne Yeh; Fiscal Year: 2002
    ..Our specific aims are to obtain atomic resolution structures of both membrane proteins, both of which are likely to be novel targets for antibiotic design and therapy. ..
  45. GLUCOSE/SECRETAGOGUE METABOLISM IN PANCREATIC ISLETS
    Michael MacDonald; Fiscal Year: 2002
    ..abstract_text> ..
  46. GLUCOSE/SECRETAGOGUE METABOLISM IN PANCREATIC ISLETS
    Michael MacDonald; Fiscal Year: 1999
    ..abstract_text> ..
  47. GLUCOSE/SECRETAGOGUE METABOLISM IN PANCREATIC ISLETS
    Michael MacDonald; Fiscal Year: 2003
    ..abstract_text> ..
  48. How Substrates Activate Fumarase and Glyoxalase Evolved
    Irwin Rose; Fiscal Year: 2004
    ..Since methylglyoxal is toxic to cells and must be removed by an active glyoxalse system, inhibition of a MSH based system could result in a treatment for many mycobacterial-based diseases such as tuberculosis. ..
  49. Glucose/Secreatogogue Metabolism in Pancreatic islets
    Michael MacDonald; Fiscal Year: 2005
    ..Resulting decreases in insulin release will be compared with alterations in metabolic pathways. In Aim 2B limited focused studies of beta ceil cataplerosis in type 2 diabetes will be performed. ..
  50. How Substrates Activate Fumarase and Glyoxalase Evolved
    Irwin Rose; Fiscal Year: 2003
    ..Since methylglyoxal is toxic to cells and must be removed by an active glyoxalse system, inhibition of a MSH based system could result in a treatment for many mycobacterial-based diseases such as tuberculosis. ..
  51. Glucose/Secreatogogue Metabolism in Pancreatic islets
    Michael MacDonald; Fiscal Year: 2006
    ..Resulting decreases in insulin release will be compared with alterations in metabolic pathways. In Aim 2B limited focused studies of beta ceil cataplerosis in type 2 diabetes will be performed. ..
  52. Structural Studies of Metabolic Membrane Proteins
    Joanne Yeh; Fiscal Year: 2006
    ..Our specific aims are to obtain atomic resolution structures of both membrane proteins, both of which are likely to be novel targets for antibiotic design and therapy. ..
  53. Structural Studies of Metabolic Membrane Proteins
    Joanne Yeh; Fiscal Year: 2005
    ..Our specific aims are to obtain atomic resolution structures of both membrane proteins, both of which are likely to be novel targets for antibiotic design and therapy. ..
  54. GLUCOSE/SECRETAGOGUE METABOLISM IN PANCREATIC ISLETS
    MICHAEL JOHN MACDONALD; Fiscal Year: 2010
    ....