laminin

Summary

Summary: Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion.

Top Publications

  1. ncbi Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures
    Jayanta Debnath
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Methods 30:256-68. 2003
  2. pmc Three-dimensional culture models of normal and malignant breast epithelial cells
    Genee Y Lee
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Nat Methods 4:359-65. 2007
  3. pmc La enhances IRES-mediated translation of laminin B1 during malignant epithelial to mesenchymal transition
    Michaela Petz
    Department of Medicine I, Division Institute of Cancer Research, Comprehensive Cancer Center Vienna, Medical University of Vienna, Borschkegasse 8a, 1090 Vienna, Austria
    Nucleic Acids Res 40:290-302. 2012
  4. doi Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511
    Sergey Rodin
    Division of Matrix Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Nat Biotechnol 28:611-5. 2010
  5. ncbi Collagen IV is essential for basement membrane stability but dispensable for initiation of its assembly during early development
    Ernst Poschl
    Department of Experimental Medicine I, University Erlangen Nurnberg, 91054 Erlangen, Germany
    Development 131:1619-28. 2004
  6. ncbi Matrigel: basement membrane matrix with biological activity
    Hynda K Kleinman
    NIH, NIDCR, 30 433, 30 Convent Dr MSC 4370, Bethesda, MD 20892 4370, USA
    Semin Cancer Biol 15:378-86. 2005
  7. pmc The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens
    Daniel Timo Behrens
    Institute for Physiological Chemistry and Pathobiochemistry, University Hospital of Munster, Munster, Germany
    J Biol Chem 287:18700-9. 2012
  8. pmc The glomerular basement membrane
    Jeffrey H Miner
    Renal Division and Dept of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Exp Cell Res 318:973-8. 2012
  9. pmc Absence of basement membranes after targeting the LAMC1 gene results in embryonic lethality due to failure of endoderm differentiation
    N Smyth
    Department of Human Anatomy and Cell Biology, University of Liverpool, Liverpool L69 3GE, United Kingdom
    J Cell Biol 144:151-60. 1999
  10. ncbi Rac1 orientates epithelial apical polarity through effects on basolateral laminin assembly
    T S Jou
    Department of Anatomy, Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    Nat Cell Biol 3:831-8. 2001

Research Grants

  1. Synaptic Laminin and the Calcium Channel
    STEVEN CARLSON; Fiscal Year: 2006
  2. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013
  3. Regulation of mural cells during pulmonary capillary formation
    Stephen E McGowan; Fiscal Year: 2013
  4. New insights in mechanisms of renal injury
    Balakuntalam S Kasinath; Fiscal Year: 2013
  5. Role of Meprin A in Acute Kidney Injury
    GUR PRASAD KAUSHAL; Fiscal Year: 2013
  6. PTH-RELATED PEPTIDE AND VIT D IN PROSTATE CANCER GROWTH
    Miriam Falzon; Fiscal Year: 2009
  7. FEASIBILITY OF REPAIRING GBM DEFECTS IN VIVO
    Jeffrey H Miner; Fiscal Year: 2012
  8. Intravenous Protein Therapy for Treatment of Congenital Muscular Dystrophy
    Bradley L Hodges; Fiscal Year: 2010
  9. Meprin A Metalloproteinase in Acute Kidney Injury
    GUR PRASAD KAUSHAL; Fiscal Year: 2013
  10. Novel Nanopolymers to Inhibit Angiogenesis and Increase the Anti-tumor Immunity
    Manuel L Penichet; Fiscal Year: 2013

Detail Information

Publications447 found, 100 shown here

  1. ncbi Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures
    Jayanta Debnath
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Methods 30:256-68. 2003
    ....
  2. pmc Three-dimensional culture models of normal and malignant breast epithelial cells
    Genee Y Lee
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Nat Methods 4:359-65. 2007
    ..Many of these crucial microenvironmental cues may be restored using three-dimensional (3D) cultures of laminin-rich extracellular matrix (lrECM)...
  3. pmc La enhances IRES-mediated translation of laminin B1 during malignant epithelial to mesenchymal transition
    Michaela Petz
    Department of Medicine I, Division Institute of Cancer Research, Comprehensive Cancer Center Vienna, Medical University of Vienna, Borschkegasse 8a, 1090 Vienna, Austria
    Nucleic Acids Res 40:290-302. 2012
    ..The translational activation of the extracellular matrix component laminin B1 (LamB1) during EMT has been recently reported suggesting an IRES-mediated mechanism...
  4. doi Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511
    Sergey Rodin
    Division of Matrix Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Nat Biotechnol 28:611-5. 2010
    ..human embryonic stem (hES) cells and induced pluripotent stem (iPS) cells on a recombinant form of human laminin-511, a component of the natural hES cell niche...
  5. ncbi Collagen IV is essential for basement membrane stability but dispensable for initiation of its assembly during early development
    Ernst Poschl
    Department of Experimental Medicine I, University Erlangen Nurnberg, 91054 Erlangen, Germany
    Development 131:1619-28. 2004
    ..Taken together with other basement membrane protein knockouts, these data suggest that laminin is sufficient for basement membrane-like matrices during early development, but at later stages the specific ..
  6. ncbi Matrigel: basement membrane matrix with biological activity
    Hynda K Kleinman
    NIH, NIDCR, 30 433, 30 Convent Dr MSC 4370, Bethesda, MD 20892 4370, USA
    Semin Cancer Biol 15:378-86. 2005
    ..In vivo, it is used for measuring angiogenic inhibitors and stimulators, to improve graft survival, repair damaged tissues, and increase tumor growth...
  7. pmc The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens
    Daniel Timo Behrens
    Institute for Physiological Chemistry and Pathobiochemistry, University Hospital of Munster, Munster, Germany
    J Biol Chem 287:18700-9. 2012
    ..A composite of two networks was obtained from fibril-free suspensions by immunobeads recognizing either laminin 332 or collagen IV...
  8. pmc The glomerular basement membrane
    Jeffrey H Miner
    Renal Division and Dept of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Exp Cell Res 318:973-8. 2012
    ..Like all basement membranes, the GBM consists mainly of laminin, type IV collagen, nidogen, and heparan sulfate proteoglycan...
  9. pmc Absence of basement membranes after targeting the LAMC1 gene results in embryonic lethality due to failure of endoderm differentiation
    N Smyth
    Department of Human Anatomy and Cell Biology, University of Liverpool, Liverpool L69 3GE, United Kingdom
    J Cell Biol 144:151-60. 1999
    The LAMC1 gene coding for the laminin gamma1 subunit was targeted by homologous recombination in mouse embryonic stem cells...
  10. ncbi Rac1 orientates epithelial apical polarity through effects on basolateral laminin assembly
    T S Jou
    Department of Anatomy, Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    Nat Cell Biol 3:831-8. 2001
    ..canine kidney (MDCK) epithelial cysts requires the small GTPase Rac1 and the basement membrane component laminin. Dominant-negative Rac1 alters the supramolecular assembly of endogenous MDCK laminin and causes a striking ..
  11. pmc Dystroglycan function requires xylosyl- and glucuronyltransferase activities of LARGE
    Kei Ichiro Inamori
    Howard Hughes Medical Institute, Department of Molecular Physiology and Biophysics, University of Iowa Roy J and Lucille A Carver College of Medicine, 4283 Carver Biomedical Research Building, 285 Newton Road, Iowa City, IA 52242 1101, USA
    Science 335:93-6. 2012
    ..This modification allowed α-DG to bind laminin-G domain-containing ECM ligands.
  12. pmc Developmental and pathogenic mechanisms of basement membrane assembly
    Peter D Yurchenco
    Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Curr Pharm Des 15:1277-94. 2009
    ..An understanding of the mechanisms of basement membrane zone assembly and resulting structure can provide insights into the development of normal tissues and the pathogenic mechanisms that underlie diverse disorders...
  13. doi Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via alphavbeta5 integrin
    Stefan R Braam
    Leiden University Medical Centre, Postal Zone S 1 P, P O Box 9600, 2300 RC Leiden, The Netherlands
    Stem Cells 26:2257-65. 2008
    ..Cells attached efficiently to natural human vitronectin, fibronectin, and Matrigel but poorly to laminin + entactin and collagen IV...
  14. ncbi Mutations in the human LARGE gene cause MDC1D, a novel form of congenital muscular dystrophy with severe mental retardation and abnormal glycosylation of alpha-dystroglycan
    Cheryl Longman
    Dubowitz Neuromuscular Centre, Imperial College, Hammersmith Campus, London, UK
    Hum Mol Genet 12:2853-61. 2003
    ..to a glycosylated epitope demonstrated a reduced molecular weight form of alpha-dystroglycan that retained some laminin binding activity...
  15. ncbi Basement membrane assembly, stability and activities observed through a developmental lens
    Peter D Yurchenco
    Department of Pathology and Laboratory Medicine, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Matrix Biol 22:521-38. 2004
    ..These studies also demonstrate that basement membrane components differentially promote cell polarization, organize and compartmentalize developing tissues, and maintain adult tissue function...
  16. ncbi Distinct target-derived signals organize formation, maturation, and maintenance of motor nerve terminals
    Michael A Fox
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 129:179-93. 2007
    ..Thus, multiple target-derived signals permit discrete control of the formation, maturation, and maintenance of presynaptic specializations...
  17. doi Laminins
    Madeleine Durbeej
    Department of Experimental Medical Science, University of Lund, Sweden
    Cell Tissue Res 339:259-68. 2010
    ..In this review, I will instead provide a snapshot of laminin structure, tissue distribution, and interactions with other matrix molecules and receptors and briefly describe ..
  18. pmc O-mannosyl phosphorylation of alpha-dystroglycan is required for laminin binding
    Takako Yoshida-Moriguchi
    Howard Hughes Medical Institute, University of Iowa Roy J and Lucille A Carver College of Medicine, 4283 Carver Biomedical Research Building, 285 Newton Road, Iowa City, IA 52242 1101, USA
    Science 327:88-92. 2010
    ..is a cell-surface glycoprotein that acts as a receptor for both extracellular matrix proteins containing laminin-G domains and certain arenaviruses...
  19. pmc Mutations in Lama1 disrupt retinal vascular development and inner limiting membrane formation
    Malia M Edwards
    The Jackson Laboratory, Bar Harbor, Maine 04609, USA
    J Biol Chem 285:7697-711. 2010
    ..mutation was identified in Lama1 that leads to the substitution of cysteine for a tyrosine at amino acid 265 of laminin alpha1, a basement membrane protein...
  20. pmc Targeted disruption of the LAMA3 gene in mice reveals abnormalities in survival and late stage differentiation of epithelial cells
    M C Ryan
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    J Cell Biol 145:1309-23. 1999
    b>Laminin 5 regulates anchorage and motility of epithelial cells through integrins alpha6beta4 and alpha3beta1, respectively...
  21. pmc The laminin family
    Monique Aumailley
    Center for Biochemistry, Medical Faculty, University of Cologne, Cologne, Germany
    Cell Adh Migr 7:48-55. 2013
    ..The human genome encodes 11 genetically distinct laminin chains...
  22. doi Autophagy is increased in laminin α2 chain-deficient muscle and its inhibition improves muscle morphology in a mouse model of MDC1A
    Virginie Carmignac
    Muscle Biology Unit, Department of Experimental Medical Science, Lund University, Sweden
    Hum Mol Genet 20:4891-902. 2011
    Congenital muscular dystrophy caused by laminin α2 chain deficiency (also known as MDC1A) is a severe and incapacitating disease, characterized by massive muscle wasting...
  23. ncbi Laminin functions in tissue morphogenesis
    Jeffrey H Miner
    Renal Division, Department of Internal Medicine and Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Annu Rev Cell Dev Biol 20:255-84. 2004
    ..These studies have shown that different laminin subunits profoundly affect tissue morphogenesis, starting around the time of embryonic implantation and extending ..
  24. doi Melanoma cells produce multiple laminin isoforms and strongly migrate on α5 laminin(s) via several integrin receptors
    Yuko Oikawa
    Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden
    Exp Cell Res 317:1119-33. 2011
    ..In the present study we have investigated the production and migration-promoting activity of laminin isoforms in melanoma...
  25. pmc The novel leptospiral surface adhesin Lsa20 binds laminin and human plasminogen and is probably expressed during infection
    Renata Siqueira Mendes
    Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503 900, Sao Paulo, SP, Brazil
    Infect Immun 79:4657-67. 2011
    ..The leptospiral protein encoded by LIC11469, named Lsa20 (leptospiral surface adhesin of 20 kDa), binds to laminin and to plasminogen...
  26. pmc Determinants of laminin polymerization revealed by the structure of the α5 chain amino-terminal region
    Sadaf Ahmahni Hussain
    Department of Life Sciences, Biophysics Section, Blackett Laboratory, Prince Consort Road, Imperial College London, London SW7 2AZ, UK
    EMBO Rep 12:276-82. 2011
    The polymerization of laminin into a cell-associated network--a key step in basement membrane assembly--is mediated by the laminin amino-terminal (LN) domains at the tips of the three short arms of the laminin αβγ-heterotrimer...
  27. pmc Proteinuria precedes podocyte abnormalities inLamb2-/- mice, implicating the glomerular basement membrane as an albumin barrier
    George Jarad
    Renal Division, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri, USA
    J Clin Invest 116:2272-9. 2006
    ..b>Laminin beta2 (LAMB2) is a GBM component required for proper functioning of the glomerular filtration barrier...
  28. pmc Laminin-binding integrins and their tetraspanin partners as potential antimetastatic targets
    Christopher S Stipp
    Departments of Biology and Molecular Physiology and Biophysics, 210 Iowa Avenue, BBE 236, University of Iowa, Iowa City, IA 52242, USA
    Expert Rev Mol Med 12:e3. 2010
    ..family of cell adhesion receptors, integrins alpha3beta1, alpha6beta1, alpha6beta4 and alpha7beta1 make up a laminin-binding subfamily...
  29. ncbi Functional structure and composition of the extracellular matrix
    Fred T Bosman
    Institut Universitaire de Pathologie, Rue du Bugnon 25, CH 1011 Lausanne, Switzerland
    J Pathol 200:423-8. 2003
    ..Matrix metalloproteinases are essential actors in this complex interplay between cells and the extracellular matrix...
  30. ncbi A highly specific inhibitor of matrix metalloproteinase-9 rescues laminin from proteolysis and neurons from apoptosis in transient focal cerebral ischemia
    Zezong Gu
    Center for Neuroscience and Aging, The Burnham Institute, La Jolla, California 92037, USA
    J Neurosci 25:6401-8. 2005
    ..2002; Horstmann et al., 2003). We determined that MMP-9 degrades the extracellular matrix protein laminin and that this degradation induces neuronal apoptosis in a transient focal cerebral ischemia model in mice...
  31. ncbi Mutations in the laminin alpha 2-chain gene (LAMA2) cause merosin-deficient congenital muscular dystrophy
    A Helbling-Leclerc
    INSERM U153, Hopital de la Pitie Salpetriere, Paris, France
    Nat Genet 11:216-8. 1995
    ..of the extracellular matrix around muscle fibres, the merosin M chain, now referred to as the alpha 2 chain of laminin-2 (ref.3). We localized this CMD locus to chromosome 6q2 by homozygosity mapping and linkage analysis...
  32. pmc Basement membrane components are key players in specialized extracellular matrices
    Jenny Kruegel
    Tissue Regeneration Work Group, Department of Prosthodontics, Georg August University, Robert Koch Str 40, 37075, Gottingen, Germany
    Cell Mol Life Sci 67:2879-95. 2010
    ..Here, we briefly review the latest research on these unique sECM and their components with a special emphasis on embryonic and adult stem cells and their niches...
  33. ncbi Tumour microenvironment: laminin 332 in squamous-cell carcinoma
    M Peter Marinkovich
    VA Medical Center, Palo Alto, California, USA
    Nat Rev Cancer 7:370-80. 2007
    ..Many studies have shown the importance of laminin 332 (previously known as laminin 5) in this process, especially in squamous cell carcinoma...
  34. ncbi Feeder-free growth of undifferentiated human embryonic stem cells
    C Xu
    Geron Corporation, 230 Constitution Drive, Menlo Park, CA 94025, USA
    Nat Biotechnol 19:971-4. 2001
    ..In this system, hES cells are cultured on Matrigel or laminin in medium conditioned by MEF...
  35. pmc Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation
    Amber N Stratman
    Department of Medical Pharmacology and Physiology, School of Medicine, Dalton Cardiovascular Center, University of Missouri, Columbia, MO 65212, USA
    Blood 114:5091-101. 2009
    ..nidogen-1 (ie, matrix-bridging proteins that link together basement membrane components) as well as perlecan and laminin isoforms...
  36. ncbi Mutations in the fukutin-related protein gene (FKRP) identify limb girdle muscular dystrophy 2I as a milder allelic variant of congenital muscular dystrophy MDC1C
    M Brockington
    Dubowitz Neuromuscular Centre, Department of Paediatrics, Faculty of Medicine, Imperial College, Hammersmith Hospital Campus, London, UK
    Hum Mol Genet 10:2851-9. 2001
    ..form of CMD (MDC1C), characterized by the inability to walk, leg muscle hypertrophy and a secondary deficiency of laminin alpha2 and alpha-dystroglycan. Both MDC1C and LGMD2I map to an identical region on chromosome 19q13.3...
  37. pmc Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells
    Takamichi Miyazaki
    Department of Embryonic Stem Cell Research, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Nat Commun 3:1236. 2012
    ..Here we show that recombinant E8 fragments of laminin isoforms (LM-E8s), which are the minimum fragments conferring integrin-binding activity, promote greater adhesion ..
  38. pmc Beta1-integrin orients epithelial polarity via Rac1 and laminin
    Wei Yu
    Department of Anatomy, School of Medicine, University of California San Francisco, San Francisco, CA 94143, USA
    Mol Biol Cell 16:433-45. 2005
    ..function-blocking antibody to the collagen matrix gives rise to cysts that have defects in the organization of laminin into the basement membrane and have inverted polarity...
  39. ncbi P0-Cre transgenic mice for inactivation of adhesion molecules in Schwann cells
    M L Feltri
    Department of Neurology, San Raffaele Scientific Institute, Milano, Italy
    Ann N Y Acad Sci 883:116-23. 1999
    ..An important component of Schwann cell basal lamina is laminin--predominantly laminins 2 and 4...
  40. doi Proliferation and plasticity of human beta cells on physiologically occurring laminin isoforms
    Meenal Banerjee
    Research Programs Unit, Molecular Neurology, Biomedicum Stem Cell Centre, University of Helsinki, Helsinki, Finland
    Mol Cell Endocrinol 355:78-86. 2012
    ..characterized the molecular composition of human islet basement membranes and shown that human beta cells bind to laminin 511 (LM511) through integrin α3β1 and Lutheran glycoprotein...
  41. pmc Cell surface proteoglycans syndecan-1 and -4 bind overlapping but distinct sites in laminin α3 LG45 protein domain
    Sonia Carulli
    Structure Fédérative de Recherche BioSciences Gerland Lyon Sud, Institut de Biologie et Chimie des Proteines, FRE 3310, CNRS, Universite Lyon 1, 7 passage du Vercors, 69367 Lyon, France
    J Biol Chem 287:12204-16. 2012
    ..receptors, syndecan-1 and -4, and the C-terminal globular domains (LG45) of the extracellular matrix protein laminin 332...
  42. ncbi Laminin alpha1-chain shows a restricted distribution in epithelial basement membranes of fetal and adult human tissues
    I Virtanen
    Department of Anatomy, University of Helsinki, Helsinki, FIN 00014, Finland
    Exp Cell Res 257:298-309. 2000
    Two novel monoclonal antibodies were raised and used to study the expression of laminin (Ln) alpha1-chain in developing and adult human tissues...
  43. ncbi Developmental regulation of the laminin alpha5 chain suggests a role in epithelial and endothelial cell maturation
    L M Sorokin
    Institute for Experimental Medicine, University of Erlangen Nürnburg, Schwabachanlage 10, 91045, Erlangen, Germany
    Dev Biol 189:285-300. 1997
    We have previously shown that mouse and bovine endothelial cells express a novel 400-kDa laminin alpha chain complexed to beta1 and gamma1 laminin chains...
  44. doi Invasion of tumorigenic HT1080 cells is impeded by blocking or downregulating the 37-kDa/67-kDa laminin receptor
    Chantal Zuber
    Laboratorium für Molekulare Biologie Genzentrum Institut für Biochemie der LMU München, Feodor Lynen Str 25, D 81377 Munchen, Germany
    J Mol Biol 378:530-9. 2008
    The 37-kDa/67-kDa laminin receptor precursor/laminin receptor (LRP/LR) acting as a receptor for prions and viruses is overexpressed in various cancer cell lines, and their metastatic potential correlates with LRP/LR levels...
  45. pmc The oriented emergence of axons from retinal ganglion cells is directed by laminin contact in vivo
    Owen Randlett
    Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UK
    Neuron 70:266-80. 2011
    ..We use time-lapse imaging to demonstrate that Laminin acting directly on RGCs is necessary and sufficient to orient axon emergence in vivo...
  46. ncbi Prognostic implication of laminin-5 gamma 2 chain expression in the invasive front of colorectal cancers, disclosed by area-specific four-point tissue microarrays
    Eiji Shinto
    Department of Pathology II, National Defense Medical College, Tokorozawa, Japan
    Lab Invest 85:257-66. 2005
    The laminin-5 gamma 2 chain (LN-5gamma2) is known to be a marker of invasion in several cancer types...
  47. pmc Endothelial basement membrane limits tip cell formation by inducing Dll4/Notch signalling in vivo
    Denise Stenzel
    Vascular Biology Laboratory, London Research Institute Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    EMBO Rep 12:1135-43. 2011
    ..Here we show that laminin α4 (Lama4) regulates tip cell numbers and vascular density by inducing endothelial Dll4/Notch signalling in vivo...
  48. doi Cell-matrix interactions in muscle disease
    Virginie Carmignac
    Muscle Biology Unit, Department of Experimental Medical Science, Lund University, Lund, Sweden
    J Pathol 226:200-18. 2012
    ..Mutations in corresponding genes cause various forms of muscular dystrophy. The muscle disorders are presented as well as advances toward the development of treatment...
  49. doi Laminin-binding integrins induce Dll4 expression and Notch signaling in endothelial cells
    Soline Estrach
    INSERM, U634, Nice F 06107, France
    Circ Res 109:172-82. 2011
    ..Because angiogenesis requires a tight coordination of these cellular processes, we hypothesized that adhesion, vascular endothelial growth factor, and Notch signaling pathways are interconnected...
  50. doi CD117-positive cells in adult human heart are localized in the subepicardium, and their activation is associated with laminin-1 and alpha6 integrin expression
    Clotilde Castaldo
    aDepartment of Biomorphological and Functional Sciences, University Federico II, Naples, Italy
    Stem Cells 26:1723-31. 2008
    ..adult normal and pathological hearts with ischemic cardiomyopathy, with respect to localization and expression of laminin and integrin isoforms...
  51. pmc Evidence for a role of tumor-derived laminin-511 in the metastatic progression of breast cancer
    Jenny Chia
    Peter MacCallum Cancer Centre, A Beckett Street, Melbourne, Victoria 8006, Australia
    Am J Pathol 170:2135-48. 2007
    ..These results are consistent with earlier reports implicating alpha3 integrins in breast cancer progression and support the role of LM-511 as a functional substrate regulating breast cancer metastasis...
  52. pmc Silencing of the laminin gamma-1 gene blocks Trypanosoma cruzi infection
    Pius N Nde
    Division of Microbial Pathogenesis and Immune Response, Department of Biomedical Sciences, School of Medicine, Meharry Medical College, 1005 Dr D B Todd Jr Blvd, Nashville, TN 37208, USA
    Infect Immun 74:1643-8. 2006
    ..cell surface trans-sialidase-like molecule that the parasite uses to attach to host cells, increases the level of laminin gamma-1 transcript and its expression in mammalian cells, leading to an increase in cellular infection...
  53. doi Integrin-dependent response to laminin-511 regulates breast tumor cell invasion and metastasis
    Nicole Kusuma
    Metastasis Research Laboratory, Peter MacCallum Cancer Centre, St Andrews Place, East Melbourne, Victoria, Australia
    Int J Cancer 130:555-66. 2012
    The basement membrane protein, laminin (LM)-511, is a potent adhesive and migratory substrate for metastatic breast tumor cells in vitro. Its expression correlates with tumor grade and metastatic potential in vivo...
  54. pmc Integrin signaling switches the cytoskeletal and exocytic machinery that drives neuritogenesis
    Stephanie L Gupton
    The Koch Institute for Integrative Cancer Research at MIT, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Dev Cell 18:725-36. 2010
    ..A second mechanism occurs in the presence of laminin through integrin-dependent activation of FAK and src and uses coordinated activity of the Arp2/3 complex and ..
  55. pmc Rotavirus enterotoxin NSP4 binds to the extracellular matrix proteins laminin-beta3 and fibronectin
    J A Boshuizen
    Laboratory of Pediatrics, Pediatric Gastroenterology and Nutrition, Erasmus MC, Rm Ee1571A, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    J Virol 78:10045-53. 2004
    ..After two-hybrid library screening, we repeatedly isolated cDNAs encoding the extracellular matrix (ECM) protein laminin-beta3 (amino acids [aa] 274 to 878) and a cDNA encoding the ECM protein fibronectin (aa 1755 to 1884)...
  56. ncbi Drug-induced readthrough of premature stop codons leads to the stabilization of laminin alpha2 chain mRNA in CMD myotubes
    Valérie Allamand
    INSERM, U582, Paris, France
    J Gene Med 10:217-24. 2008
    The most common form of congenital muscular dystrophy is caused by a deficiency in the alpha2 chain of laminin-211, a protein of the extracellular matrix...
  57. ncbi Laminin gamma2 mediates Wnt5a-induced invasion of gastric cancer cells
    Hideki Yamamoto
    Department of Biochemistry, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
    Gastroenterology 137:242-52, 252.e1-6. 2009
    ..The aim of this study was to show that Wnt5a is involved in metastasis of gastric cancer cells in vivo and to explore the molecular mechanism by which Wnt5a regulates migration and invasion...
  58. ncbi Differing modes of tumour cell invasion have distinct requirements for Rho/ROCK signalling and extracellular proteolysis
    Erik Sahai
    Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK
    Nat Cell Biol 5:711-9. 2003
    ..Combined blockade of extracellular proteases and ROCK negates the ability of tumour cells to switch between modes of motility and synergises to prevent tumour cell invasion...
  59. pmc Roles for laminin in embryogenesis: exencephaly, syndactyly, and placentopathy in mice lacking the laminin alpha5 chain
    J H Miner
    Department of Medicine, Renal Division, St Louis, Missouri, USA
    J Cell Biol 143:1713-23. 1998
    ..Previously described mutations in laminin chain genes result in diverse disorders that are manifested postnatally and therefore provide little insight into ..
  60. ncbi Integrins as receptors for laminins
    A M Belkin
    Department of Biochemistry, The Holland Laboratory, American Red Cross, Rockville, Maryland 20855, USA
    Microsc Res Tech 51:280-301. 2000
    ..1 beta 1, alpha 2 beta 1, alpha 3 beta 1, alpha 6 beta 1, alpha 7 beta 1 and alpha 6 beta 4 heterodimers serve as laminin receptors on a variety of cell types...
  61. pmc Endothelial cell laminin isoforms, laminins 8 and 10, play decisive roles in T cell recruitment across the blood-brain barrier in experimental autoimmune encephalomyelitis
    M Sixt
    Interdisciplinary Center for Clinical Research IZKF, Nikolaus Fiebiger Center, University of Erlangen Nuremberg, Glückstr 6, 91054 Erlangen, Germany
    J Cell Biol 153:933-46. 2001
    ..Endothelial basement membranes contained laminin 8 (alpha4beta1gamma1) and/or 10 (alpha5beta1gamma1) and their expression was influenced by proinflammatory ..
  62. ncbi Profiles of matrix metalloproteinases, their inhibitors, and laminin in stroke patients: influence of different therapies
    Solveig Horstmann
    Department of Neurology, Medical School, University of Heidelberg, Germany
    Stroke 34:2165-70. 2003
    ..to determine the temporal profile of several matrix metalloproteinases (MMPs), tissue inhibitors of MMPs (TIMPs), and laminin (an MMP substrate) in human stroke under different treatment paradigms, including thrombolysis and hypothermia.
  63. ncbi Molecular cloning of a novel laminin chain, alpha 5, and widespread expression in adult mouse tissues
    J H Miner
    Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 270:28523-6. 1995
    We have identified a fifth member of the alpha subfamily of vertebrate laminin chains...
  64. pmc Matrix assembly, regulation, and survival functions of laminin and its receptors in embryonic stem cell differentiation
    Shaohua Li
    Department of Pathology and Laboratory Medicine, University of Medicine and Dentistry of New Jersey UMDNJ, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Cell Biol 157:1279-90. 2002
    b>Laminin-1 is essential for early embryonic basement membrane assembly and differentiation. Several steps can be distinguished, i.e...
  65. ncbi Tumor-infiltrating dendritic cell precursors recruited by a beta-defensin contribute to vasculogenesis under the influence of Vegf-A
    Jose R Conejo-Garcia
    Center for Research in Reproduction and Women s Health, University of Pennsylvania Medical Center, BRBII III, 421 Curie Blvd, Philadelphia, Pennsylvania 19104, USA
    Nat Med 10:950-8. 2004
    ....
  66. pmc Mutations in the fukutin-related protein gene (FKRP) cause a form of congenital muscular dystrophy with secondary laminin alpha2 deficiency and abnormal glycosylation of alpha-dystroglycan
    M Brockington
    The Dubowitz Neuromuscular Centre, Department of Paediatrics, Imperial College School of Medicine, Hammersmith Hospital Campus, London, United Kindom
    Am J Hum Genet 69:1198-209. 2001
    ..Approximately 40% of patients with CMD have a primary deficiency (MDC1A) of the laminin alpha2 chain of merosin (laminin-2) due to mutations in the LAMA2 gene...
  67. ncbi Review: Lutheran/B-CAM: a laminin receptor on red blood cells and in various tissues
    Yamato Kikkawa
    Department of Pathophysiology, Cancer Research Institute, Sapparo Medical University, School of Medicine, Sapparo, Japan
    Connect Tissue Res 46:193-9. 2005
    ..Recently, several groups have reported that Lu/B-CAM is a novel receptor for laminin a5...
  68. pmc Deletion of the laminin alpha4 chain leads to impaired microvessel maturation
    Jill Thyboll
    Division of Matrix Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 17 177 Stockholm, Sweden
    Mol Cell Biol 22:1194-202. 2002
    The laminin alpha4 chain, a component of laminin-8 and -9, is expressed in basement membranes, such as those beneath endothelia, the perineurium of peripheral nerves, and around developing muscle fibers...
  69. ncbi Primary structure of dystrophin-associated glycoproteins linking dystrophin to the extracellular matrix
    O Ibraghimov-Beskrovnaya
    Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242
    Nature 355:696-702. 1992
    ..glycoproteins (DAGs) are encoded by a single messenger RNA and the extracellular 156K DAG binds laminin. Thus, the 156K DAG is a new laminin-binding glycoprotein which may provide a linkage between the sarcolemma and ..
  70. pmc Laminin-10 is crucial for hair morphogenesis
    Jie Li
    Program in Epithelial Biology, Stanford University School of Medicine, 269 Campus Drive, Room 2145, CA 94305, USA
    EMBO J 22:2400-10. 2003
    ..Here, we describe the essential role of laminin-10 (alpha5beta1gamma1) in hair follicle development...
  71. ncbi The LG1-3 tandem of laminin alpha5 harbors the binding sites of Lutheran/basal cell adhesion molecule and alpha3beta1/alpha6beta1 integrins
    Yamato Kikkawa
    Renal Division, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:14853-60. 2007
    The laminin-type globular (LG) domains of laminin alpha chains have been implicated in various cellular interactions that are mediated through receptors such as integrins, alpha-dystroglycan, syndecans, and the Lutheran blood group ..
  72. ncbi Laminin-induced signaling in tumor cells: the role of the M(r) 67,000 laminin receptor
    Vered Givant-Horwitz
    Department of Pharmacology, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel
    Cancer Res 64:3572-9. 2004
    The expression of the M(r) 67,000 laminin receptor, a nonintegrin laminin receptor, was found to be up-regulated in neoplastic cells and to directly correlate with invasion and metastatic potential...
  73. ncbi Compositional and structural requirements for laminin and basement membranes during mouse embryo implantation and gastrulation
    Jeffrey H Miner
    Renal Division, Washington University School of Medicine, St Louis, MO 63110, USA
    Development 131:2247-56. 2004
    ..b>Laminin 1 (alpha1beta1gamma1) is a major laminin found at early stages of embryogenesis in both embryonic and ..
  74. ncbi High glucose, high insulin, and their combination rapidly induce laminin-beta1 synthesis by regulation of mRNA translation in renal epithelial cells
    Meenalakshmi M Mariappan
    Department of Medicine, MC7882, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
    Diabetes 56:476-85. 2007
    b>Laminin is a glycoprotein that contributes to renal extracellular matrix expansion in diabetes...
  75. ncbi Laminin alpha 5 is required for lobar septation and visceral pleural basement membrane formation in the developing mouse lung
    Nguyet M Nguyen
    Pulmonary and Critical Care Medicine Division, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Dev Biol 246:231-44. 2002
    b>Laminin alpha/beta/gamma heterotrimers are the major noncollagenous components of all basement membranes. To date, five alpha, three beta, and three gamma chains have been identified...
  76. pmc Calcium channels link the muscle-derived synapse organizer laminin β2 to Bassoon and CAST/Erc2 to organize presynaptic active zones
    Jie Chen
    Department of Anatomy and Cell Biology and Kansas Intellectual and Developmental Disabilities Research Center, University of Kansas Medical School, Kansas City, Kansas 66160, USA
    J Neurosci 31:512-25. 2011
    ..At the synaptic cleft of double knock-out mice, we also observed a decrease of the synaptic organizer laminin β2 protein, an extracellular ligand of P/Q- and N-type VDCCs...
  77. ncbi Laminin isoforms and their integrin receptors in glioma cell migration and invasiveness: Evidence for a role of alpha5-laminin(s) and alpha3beta1 integrin
    Tomoyuki Kawataki
    Department of Odontology and Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, S 141 04 Stockholm, Sweden
    Exp Cell Res 313:3819-31. 2007
    ..In functional assays, alpha3 (Lm-332/laminin-5)- and alpha5 (Lm-511/laminin-10)-laminins strongly promoted migration of all glioma cell lines tested...
  78. pmc A role for pericytes as microenvironmental regulators of human skin tissue regeneration
    Sophie Paquet-Fifield
    Research Division, Peter MacCallum Cancer Centre, St Andrew s Place, Melbourne, Victoria, Australia
    J Clin Invest 119:2795-806. 2009
    ..Using this approach, we identified as a candidate the gene LAMA5, which encodes laminin alpha5, a subunit of the ECM component laminin-511/521 (LM-511/521)...
  79. pmc Laminin expression in adult and developing retinae: evidence of two novel CNS laminins
    R T Libby
    Department of Biology, Boston College, Chestnut Hill, Massachusetts 02467, USA
    J Neurosci 20:6517-28. 2000
    ..Here, we examine the expression of all known laminin chains within one component of the CNS, the retina...
  80. ncbi The laminin alpha2 expressed by dystrophic dy(2J) mice is defective in its ability to form polymers
    H Colognato
    Department of Pathology, Robert Wood Johnson Medical School, Piscataway, 08854, USA
    Curr Biol 9:1327-30. 1999
    ..Mice homozygous for the dy(2J) allele of LAMA2 express a laminin alpha2 subunit that has a deletion in the amino-terminal domain VI, providing an animal model for study of the ..
  81. doi The 67 kDa laminin receptor: structure, function and role in disease
    John Nelson
    School of Biological Sciences, Queen s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UK
    Biosci Rep 28:33-48. 2008
    The 67LR (67 kDa laminin receptor) is a cell-surface receptor with high affinity for its primary ligand...
  82. ncbi LARGE can functionally bypass alpha-dystroglycan glycosylation defects in distinct congenital muscular dystrophies
    Rita Barresi
    Howard Hughes Medical Institute, Department of Physiology and Biophysics, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    Nat Med 10:696-703. 2004
    ..dystrophies restores alpha-DG receptor function, whereby glycan-enriched alpha-DG coordinates the organization of laminin on the cell surface...
  83. pmc Basal lamina strengthens cell membrane integrity via the laminin G domain-binding motif of alpha-dystroglycan
    Renzhi Han
    Howard Hughes Medical Institute, Department of Molecular Physiology, The University of Iowa, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 106:12573-9. 2009
    ..are recapitulated by Large(myd) muscles, which have an intact dystrophin-glycoprotein complex and lack only the laminin globular domain-binding motif on alpha-dystroglycan...
  84. pmc Laminin-111 protein therapy prevents muscle disease in the mdx mouse model for Duchenne muscular dystrophy
    Jachinta E Rooney
    Department of Pharmacology, University of Nevada School of Medicine, Reno, NV 89557, USA
    Proc Natl Acad Sci U S A 106:7991-6. 2009
    ..The alpha(7)beta(1)-integrin is a laminin-binding protein up-regulated in the skeletal muscle of DMD patients and in the mdx mouse model...
  85. pmc Structure-guided identification of a laminin binding site on the laminin receptor precursor
    Kelly V Jamieson
    Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
    J Mol Biol 405:24-32. 2011
    The 37/ 67-kDa human laminin receptor (LamR) is a cell surface receptor for laminin, prion protein, and a variety of viruses. Because of its wide range of ligands, LamR plays a role in numerous pathologies...
  86. pmc Mutations in the human laminin beta2 (LAMB2) gene and the associated phenotypic spectrum
    Verena Matejas
    Institute of Human Genetics, University Hospital Erlangen, University of Erlangen Nuremberg, Germany
    Hum Mutat 31:992-1002. 2010
    ..LAMB2 encodes the basement membrane protein laminin beta2, which is incorporated in specific heterotrimeric laminin isoforms and has an expression pattern ..
  87. doi Laminin regulates postnatal oligodendrocyte production by promoting oligodendrocyte progenitor survival in the subventricular zone
    Jenne Relucio
    Department of Pharmacology, Stony Brook University, Stony Brook, New York, 11794, USA
    Glia 60:1451-67. 2012
    The laminin family of extracellular matrix proteins are expressed broadly during embryonic brain development, but are enriched at ventricular and pial surfaces where laminins mediate radial glial attachment during corticogenesis...
  88. pmc Mouse acetylcholinesterase enhances neurite outgrowth of rat R28 cells through interaction with laminin-1
    Laura E Sperling
    Entwicklungsbiologie und Neurogenetik, Fachbereich Biologie, Technische Universitat Darmstadt, Darmstadt, Germany
    PLoS ONE 7:e36683. 2012
    ..Here, we investigated the role of AChE binding to laminin-1 on the regulation of neurite outgrowth by using cell culture, immunocytochemistry, and molecular biological ..
  89. pmc PDGF enhances IRES-mediated translation of Laminin B1 by cytoplasmic accumulation of La during epithelial to mesenchymal transition
    Michaela Petz
    Department of Medicine I, Institute of Cancer Research, Comprehensive Cancer Center Vienna, Medical University of Vienna, Borschkegasse 8a, 1090 Vienna, Austria
    Nucleic Acids Res 40:9738-49. 2012
    The extracellular matrix protein Laminin B1 (LamB1) regulates tumor cell migration and invasion...
  90. pmc Nrk2b-mediated NAD+ production regulates cell adhesion and is required for muscle morphogenesis in vivo: Nrk2b and NAD+ in muscle morphogenesis
    Michelle F Goody
    School of Biology and Ecology, University of Maine, Orono, ME 04469, USA
    Dev Biol 344:809-26. 2010
    ..Nrk2b is required for normal Laminin polymerization at the myotendinous junction (MTJ)...
  91. pmc Crystal structures of the network-forming short-arm tips of the laminin β1 and γ1 chains
    Federico Carafoli
    Department of Life Sciences, Imperial College London, London, United Kingdom
    PLoS ONE 7:e42473. 2012
    ..The three short arms of the cross-shaped laminin molecule are composed of one chain each and their tips mediate the formation of a polymeric network...
  92. doi Integrin-laminin interactions controlling neurite outgrowth from adult DRG neurons in vitro
    Stefan Plantman
    Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
    Mol Cell Neurosci 39:50-62. 2008
    ..in the nervous system occur in the adult, we have examined neurite outgrowth from adult mouse DRG neurons on four laminin isoforms (laminin-1/LM-111, laminin-2/LM-211, laminin-8/LM-411 and laminin-10/LM-511) in vitro...
  93. pmc Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel
    Liang Jin
    Department of Diabetes and Metabolic Diseases Research, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 110:3907-12. 2013
    ..When cells grown in Matrigel are then transferred to a Matrigel-free semisolid medium with a unique laminin-based hydrogel, some cells grow and differentiate into another type of colony, which we name "Endocrine/Acinar...
  94. ncbi Cell type-specific differences in glycosaminoglycans modulate the biological activity of a heparin-binding peptide (RKRLQVQLSIRT) from the G domain of the laminin alpha1 chain
    M P Hoffman
    Craniofacial Developmental Biology and Regeneration Branch, NIDCR, National Institutes of Health, Bethesda, Maryland 20892 4370, USA
    J Biol Chem 276:22077-85. 2001
    AG73 (RKRLQVQLSIRT), a peptide from the G domain of the laminin alpha1 chain, has diverse biological activities with different cell types...
  95. ncbi Expression of plasminogen activator inhibitor-1, urokinase receptor and laminin gamma-2 chain is an early coordinated event in incipient oral squamous cell carcinoma
    Pia Lindberg
    Department of Oral Pathology, Center for Oral Health Science, Malmo University, Sweden
    Int J Cancer 118:2948-56. 2006
    ..of plasminogen activator inhibitor-1 (PAI-1) and urokinase receptor (uPAR) together with the gamma2-chain of laminin-5 (lam-gamma2) by immunohistochemistry in 20 cases with incipient oral squamous cell carcinoma (SCC)...
  96. doi Laminin-511 but not -332, -111, or -411 enables mouse embryonic stem cell self-renewal in vitro
    Anna Domogatskaya
    Department of Medical Biochemistry and Biophysics, Division of Matrix Biology, Karolinska Institute, Stockholm, Sweden
    Stem Cells 26:2800-9. 2008
    We tested specific laminin (LN) isoforms for their ability to serve as substrata for maintaining mouse embryonic stem (ES) cells pluripotent in vitro in the absence of leukemia inhibitory factor or any other differentiation inhibitors or ..
  97. pmc Loss of cell-surface laminin anchoring promotes tumor growth and is associated with poor clinical outcomes
    Armin Akhavan
    California Pacific Medical Center Research Institute, San Francisco, CA, USA
    Cancer Res 72:2578-88. 2012
    ..at the cell surface enables assembly and signaling of many ECMs, but the possible contributions of altered laminin anchoring to cancer progression remain undetermined...
  98. pmc Adhesion and migration, the diverse functions of the laminin alpha3 subunit
    Kevin J Hamill
    Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Tarry 8 746, Chicago, IL 60611, USA
    Dermatol Clin 28:79-87. 2010
    ..This article provides an overview of the gene, transcripts, and protein structures of laminin alpha3. Also discussed are the proposed functions for the alpha3 subunit-containing laminins.
  99. doi New nerve regeneration strategy combining laminin-coated chitosan conduits and stem cell therapy
    Sung Hao Hsu
    Institute of Zoology, College of Life Science, National Taiwan University, Taipei, Taiwan, ROC
    Acta Biomater 9:6606-15. 2013
    ..We designed a laminin (LN)-modified chitosan multi-walled nerve conduit combined with bone marrow stem cell (BMSC) grating to bridge a ..
  100. pmc Laminin is required to orient epithelial polarity in the C. elegans pharynx
    Jeffrey P Rasmussen
    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Development 139:2050-60. 2012
    ..We show that laminin, but not other basement membrane components, orients the polarity of the double plate cells...
  101. ncbi Proinflammatory cytokines TNF-alpha and IFN-gamma alter laminin expression under an apoptosis-independent mechanism in human intestinal epithelial cells
    Caroline Francoeur
    Canadian Institutes of Health Research Group in Functional Development and Physiopathology of the Digestive Tract, Département d Anatomie et de Biologie Cellulaire, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, Quebec, Canada J1H 5N4
    Am J Physiol Gastrointest Liver Physiol 287:G592-8. 2004
    ..In the normal small intestine, laminin-5 and -10 are mainly expressed at the base of villus cells...

Research Grants73

  1. Synaptic Laminin and the Calcium Channel
    STEVEN CARLSON; Fiscal Year: 2006
    ..We have found a transmembrane link between a synaptic laminin, containing alpha4, Beta2, and gamma1chains, and the voltage-gated calcium channel at the electric organ synapse, ..
  2. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013
    ..Further, we found that chymase added to adult dog cardiomyocytes (plated on laminin) resulted in direct cleavage of laminin, focal adhesion kinase (FAK) dephopsphorylation, MMP release and myosin ..
  3. Regulation of mural cells during pulmonary capillary formation
    Stephen E McGowan; Fiscal Year: 2013
    ..Detailed time-lapse microscopic studies will reveal how laminin and integrins promote PDGF-A or PDGF-B-stimulated migration of fibroblasts and pericytes...
  4. New insights in mechanisms of renal injury
    Balakuntalam S Kasinath; Fiscal Year: 2013
    ..This occurs in association with renal hypertrophy, increase in renal matrix laminin beta1 and fibronectin expression, and albuminuria. 2...
  5. Role of Meprin A in Acute Kidney Injury
    GUR PRASAD KAUSHAL; Fiscal Year: 2013
    ..kidney cortex capable of degrading the extracellular matrix (ECM) proteins including collagen IV, fibronectin, laminin, and nidogen in vitro...
  6. PTH-RELATED PEPTIDE AND VIT D IN PROSTATE CANCER GROWTH
    Miriam Falzon; Fiscal Year: 2009
    ..prostate cancer cell (a) proliferation;(b) adhesion to the extracellular matrix proteins collagen types I and IV, laminin, and fibronectin;(c) migration onto laminin and collagen type I;(d) invasion of Matrigel, a reconstituted ..
  7. FEASIBILITY OF REPAIRING GBM DEFECTS IN VIVO
    Jeffrey H Miner; Fiscal Year: 2012
    ..The GBM's major components are laminin-521 (alpha5beta2gamma1), type IV collagen (the alpha3/4/5 network), nidogens-1 and -2, and the heparin sulfate ..
  8. Intravenous Protein Therapy for Treatment of Congenital Muscular Dystrophy
    Bradley L Hodges; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Our objective is to develop a laminin isoform as an intravenously delivered protein therapy for treatment of Congenital Muscular Dystrophy Type IA (MCD1A)...
  9. Meprin A Metalloproteinase in Acute Kidney Injury
    GUR PRASAD KAUSHAL; Fiscal Year: 2013
    ..kidney cortex capable of degrading the extracellular matrix (ECM) proteins including collagen IV, fibronectin, laminin, and nidogen in vitro...
  10. Novel Nanopolymers to Inhibit Angiogenesis and Increase the Anti-tumor Immunity
    Manuel L Penichet; Fiscal Year: 2013
    ..The anti-angiogenic effect would be achieved using AON to inhibit the expression of vascular protein laminin-411 and/or vascular endothelial growth factor (VEGF), critical factors produced by the cancer cells needed to ..
  11. Matricellular Proteins in Trabecular Meshwork Increase Intraocular Pressure
    Douglas J Rhee; Fiscal Year: 2013
    ..Our preliminary data indicate the SPARC overexpression increases collagen IV, laminin, and fibronectin production and shifts the balance of matrix metalloproteinases and tissue inhibitors of ..
  12. Molecular interactions: oral bacteria & matrix proteins
    Keith Mintz; Fiscal Year: 2010
    ..The bacterium also binds to the ECM proteins, collagen, fibronectin and laminin. Using a genetic approach, we have identified the first A...
  13. Congenital Myasthenic Syndromes: Pathogenic Mechanisms
    RICARDO ANIBAL MASELLI; Fiscal Year: 2013
    ..We recently reported an additional target for mutations causing CMS, which is the gene encoding laminin beta 2 (LAMB2), bringing up the number of genes associated with CMS, to a total of twelve...
  14. BIOACTIVITIES OF PNEUMOCOCCAL CELL WALL IN MENINGITIS
    Elaine I Tuomanen; Fiscal Year: 2013
    ..infection: We determined how the pneumococcus localizes to the blood brain barrier (CbpA on the cell wall ligates laminin receptor LR) and then penetrates into the CNS (phosphorylcholine PCho on the cell wall ligates platelet ..
  15. Combinatorial Metal Oxide Substrates to Enhance Osteogenic Stem Cell Functions
    Jaime E Ramírez-Vick; Fiscal Year: 2012
    ..distribution and conformation of select serum and/or ECM adhesive proteins such as collagen type I, fibronectin, laminin, and vitronectin, adsorbed onto the novel material substrates prepared by the research activities pertinent to ..
  16. Hit to Lead Optimization of Small Molecule Therapeutics for Muscular Dystrophy
    Bradley L Hodges; Fiscal Year: 2010
    ..membrane proteins such as the sarcoglycans and alpha dystroglycan, or extracellular matrix proteins such as laminin-211 and collagen VI...
  17. Bioengineered scaffolds for peripheral nerve repair
    Ravi V Bellamkonda; Fiscal Year: 2010
    ..we proposed to design 3D hydrogel based scaffolds with either isotropically or anisotropically distributed ECM (laminin-1) or nerve growth factor (NGF) to bridge gaps longer than 15mm in rats...
  18. MOLECULAR ASPECTS OF ALPORT RENAL DISEASE PROGRESSION
    DOMINIC E COSGROVE; Fiscal Year: 2013
    ..disease initiation results from altered signaling through 1321 integrin which activates expression of abnormal laminin in podocytes...
  19. DEFINITION OF MICROENVIRONMENT IN BREAST CANCER
    Mina Bissell; Fiscal Year: 2013
    ..and functional cues to luminal epithelial cells (LEPs) partly via production of, and signaling through, laminin-111 (Ln-1)...
  20. Cell-Matrix Interactions in Epithelial Polarization
    Karl S Matlin; Fiscal Year: 2010
    ..Of these, isoforms of the basement membrane protein laminin are likely to play a crucial role...
  21. LAMININS AND GLOMERULAR FILTRATION
    Jeffrey H Miner; Fiscal Year: 2013
    ..The GBM contains laminin, collagen IV, nidogen, and the heparan sulfate proteoglycan agrin...
  22. NOVEL FUNCTIONS OF RED CELL PROTEINS LU AND LW
    Joel A Chasis; Fiscal Year: 2010
    ..adhesion mediates erythroblast-macrophage attachment;and normal but not Lu null erythroblasts bind matrix laminin in a differentiation stage-specific manner...
  23. Nanoconjugate based on polymalic acid for brain tumor treatment
    Julia Y Ljubimova; Fiscal Year: 2013
    ..unit;Morpholino antisense oligonucleotide (AON) unit targeting two chains of angiogenic glioma-increased protein laminin 411;anti-transferrin receptor antibody for specific tissue targeting and endosomal uptake;biopolymer protector ..
  24. Intracellular Invasion by Streptococcus mutans: Significance in Disease
    JOSE A LEMOS; Fiscal Year: 2013
    ..Invasion of HCAEC was associated with an ability to avidly bind to collagen and laminin, and invasive strains were significantly more virulet than non-invasive strains in the Galleria mellonella model ..
  25. Genetic Epidemiology of Pelvic Organ Prolapse
    Jennifer Marie Wu; Fiscal Year: 2010
    ..A study of familial prolapse identified a single nucleotide polymorphism (SNP) in the LAMC1 gene encoding laminin, an ECM component...
  26. High-throughput genetic & small-molecule screening for therapeutic modifiers
    Peter G Schultz; Fiscal Year: 2010
    ..glycoprotein that acts as a high- affinity receptor for both extracellular matrix proteins containing laminin-G domains and certain arenaviruses...
  27. Manipulating b1 integrin to enhance radiation therapy for breast cancer
    Catherine C Park; Fiscal Year: 2012
    ..after IR exposure in a heritable and persistent manner in mammary epithelial cells cultured in a 3- dimensional laminin rich matrix (3D IrECM), (Appendix II)...
  28. Human Prostate Cancer Metastasis and Laminin Binding Integrins
    Anne E Cress; Fiscal Year: 2013
    ..Specifically, we will identify the role of laminin binding integrins A6B1, A6pB1 and A3B1 at the molecular and cellular level using both in vitro tissue culture and ..
  29. BASEMENT MEMBRANE SELF-ASSEMBLY AND STRUCTURE
    PETER DANA YURCHENCO; Fiscal Year: 2013
    ..It seeks to determine if cell polarization depends upon laminin- initiated organization of the basal cell surfaces, if b1-integrin activity depends on a coding or summation of ..
  30. Biomimetic Engineering of Vascular Prostheses
    Roger E Marchant; Fiscal Year: 2012
    ..The polymer gel will incorporate RGD, and SMC-binding 1-dystroglycan and laminin peptides presented on pendant dendrons...
  31. Characterization of alphavirus infection receptors
    William B Klimstra; Fiscal Year: 2009
    ..the utilization of previously characterized cell surface receptor molecules heparan sulfate and the high affinity laminin receptor using well-characterized cell lines...
  32. Mechanisms Modulating Cytoskeletal Dynamics During Schwann Cell Myelination
    CRISTINA MARIA FERNANDEZ VALLE; Fiscal Year: 2010
    ..In Aim 2, we will assess whether neuregulin and laminin regulate LlMK and cofilin phosphorylation...
  33. Time lapse imaging of serotonin axon regeneration in the neocortex of adult mouse
    David J Linden; Fiscal Year: 2013
    ..levels of integrin [unreadable]1, a protein that forms part of the receptor for the permissive growth substrate laminin. We shall cross mouse strains: floxed integrin [unreadable]1 with serotonin transporter-driven Cre, to ..
  34. PAR-1b in Integrin Localization and Laminin-111 Assembly in the Salivary Gland
    ELISE GERVAIS; Fiscal Year: 2013
    ..also shown that providing epithelial cells with an artificial matrix coated with the basement membrane protein laminin-111 also enhances cellular polarity...
  35. Molecular basis of age-related synaptic alterations
    Gregorio Valdez; Fiscal Year: 2010
    ..Concomitant with these morphological changes, three synaptic adhesion molecules, laminin alpha4, laminin beta2 and agrin, are significantly decreased in aged NMJs...
  36. Formation of bile ducts in three dimensional culture
    Keith E Mostov; Fiscal Year: 2012
    ..We use a stable mouse cell line termed Hepatic Progenitor cells Proliferating on Laminin, (HPPL), which is capable of differentiating into hepatocytes and cholangiocytes...
  37. Preclinical Testing of Integrin Enhancing Molecules for the Treatment of Muscular
    Dean J Burkin; Fiscal Year: 2011
    ..in the gene encoding dystrophin resulting in an absence of the dystrophin protein, a critical component of the laminin binding dystrophin glycoprotein complex in muscle...
  38. Role of alpha6 beta1 Integrin in Prostate Cancer
    CYNTHIA K MIRANTI; Fiscal Year: 2013
    ..lines will be used to decipher the transcriptional and post-transcriptional mechanisms by which AR, Erg/Etv, and laminin-10 control integrin [unreadable]6[unreadable]1 expression in prostate cancer...
  39. Significance of Microenvironment for Prostate Cancer Initiation and Progression
    Stephen R Plymate; Fiscal Year: 2010
    ..Aim 3. Juxtacrine signaling models involving tumor and senescent fibroblasts. 3. Laminin Dysregulation in Prostate Cancer: Aim1...
  40. Role of Extracellular Matrix in Retinal Development and Disease
    William J Brunken; Fiscal Year: 2012
    ..We produced mice with null mutations in two laminin genes, Lamb2 and Lamc3, and found that these molecules are necessary for normal retinal development...
  41. Automated Analysis of Skeletal Muscle Fiber Cross-sectional Area and Metabolic Ty
    Patrick M McDonough; Fiscal Year: 2012
    ..1) to develop monoclonal antibodies (MAbs) for identification of myosin subtypes (slow, IIa, IIb, and IIx), laminin, and OXPAT in the mouse, and to label the MAbs with organic fluorophores or nanocrystals (aka quantum dots) for ..
  42. Laminins in the Skin
    MATT PETER MARINKOVICH; Fiscal Year: 2013
    ..Work by our group during the current funding cycle implicates an essential role for laminin-511 in hair and appendage morphogenesis in the skin...
  43. Probing biochemical/biophysical influences on endothelial-mesenchymal transition
    MICHAEL PAUL SCHWARTZ; Fiscal Year: 2013
    ..in EndMT during tissue development, including proteoglycans [9], vitronectin, collagen, fibronectin and laminin [10-11]...
  44. Regulation of SMG Development by Adhesion Receptors
    A Sue Menko; Fiscal Year: 2012
    ..have focused on E-cadherin, a principal salivary cell-cell adhesion receptor, and 1321 integrin, the receptor for laminin, a major component of the basement membrane in the SMG...
  45. Genetically Designed Materials for Cartilage Repair
    Lori A Setton; Fiscal Year: 2010
    ..unique features of immature NP cells to be their adhesion to specific laminins, and their expression of laminin-binding receptors and laminin-associated proteins...
  46. The Laminin Receptors in Kidney Development
    Roy Zent; Fiscal Year: 2013
    ..Integrins can be classified by these ligand binding properties into collagen, laminin and RGD binding receptors. The principal laminin binding integrins are 1321, 1621 and 1624...
  47. Paracrine regulation of angiogenesis through integrin crosstalk
    WHITNEY MORGAN LONGMATE; Fiscal Year: 2013
    ..Indeed, while the [unreadable]3[unreadable]1 ligand, laminin-332 (LN-332), is deposited by activated keratinocytes immediately upon wounding, [unreadable]9[unreadable]1 ..
  48. Physiologic Study of Lymphatic Metastasis
    VICENTE ANDRES RESTO; Fiscal Year: 2013
    ..Additionally, our preliminary work has shown that extracellular elements ofthe lymph node parenchyma, such as laminin, similarly support binding of tumor cells under these same conditions...
  49. MECHANISMS IN THE REMODELING OF LUNG STRUCTURE
    Michael J Holtzman; Fiscal Year: 2012
    ..Focal points of the proposed studies will be laminin-332 (laminin-5), surfactant protein D (SP-D), matrilysin (MMP-7), serglycin, and a distinct population of non-B ..
  50. Substrate stiffness regulates alveolar epithelial cell behavior
    Jonathan C Jones; Fiscal Year: 2013
    ..that the tissue in fibrosis induces alveolar epithelial cells (AEC) to deposit matrix enriched in the (3 laminin subunit which functions, via the cell surface receptor dystroglycan and the signaling scaffold protein plectin, ..
  51. Mutant Laminin Chains and Nephrotic Syndrome
    YING MAGGIE CHEN; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Laminin (2 (LAMB2) is a component of Laminin-521, the major laminin trimer of the kidney glomerular basement membrane (GBM)...
  52. Integrin Alleviation of Muscular Dystrophy
    Dean J Burkin; Fiscal Year: 2012
    ..The 1721 integrin is a major laminin receptor expressed in satellite cells, myoblasts and vascular smooth muscle...
  53. Microenivironment Dimensionality Modulates Neuronal Signaling
    Jennie B Leach; Fiscal Year: 2012
    ..we will first investigate the roles of 1 integrin, vinculin, FAK and pFAK in DRG neurite outgrowth in 3D laminin culture scaffolds (Aim 1)...
  54. Neuromuscular synapse remodeling by glial cells in aging mice
    Yi Zuo; Fiscal Year: 2012
    ..Aim 2 examines the molecular mechanism underlying aging-related synapse loss, and tests whether changes in laminin-TSC interactions lead to invasion of TSCs between muscle fibers and nerve terminals, and whether this leads to ..
  55. Receptor Decoy Inhibitor of Anthrax Toxin
    John A T Young; Fiscal Year: 2012
    ..its pharmacokinetic properties by disrupting its interaction with its physiological ligands (collagen IV and laminin), and by exploiting PEGylation and Ig fusion protein approaches...
  56. Microenvironment mediated drug resistance in melanoma.
    Keiran Smalley; Fiscal Year: 2013
    ..of PDGF-D, VEGF and the Notch ligand Jagged as well as increasing the deposition of fibronectin, collagens and laminin. The first aim will investigate how BRAF inhibition leads to the acquisition of autocrine ECM-driven signaling ..
  57. Mechanisms of Pancreatic Tumor Nerve Invasion
    Isis C Sroka; Fiscal Year: 2013
    ..The central hypothesis is that laminin dependent nerve invasion during pancreatic cancer metastasis is dependent upon A6B1...
  58. Functional analyses of a member of a novel family of laminin-related proteins
    Kevin J Hamill; Fiscal Year: 2012
    ..We recently reported the identification of a novel family of proteins, termed the LaNts, which are related to the laminin and netrin families of extracellular matrix proteins...
  59. Polymeric Matrices with Defined Cell Adhesion
    David J Mooney; Fiscal Year: 2013
    ..g., fibronectin, collagen, laminin) in order to identify materials with high specificity for MSC adhesion and possibly distinct stages of ..
  60. Alpha6 integrin complexes and tumor progression
    Martin E Hemler; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The laminin binding integrins (LB integrins) play critical roles during carcinoma initiation, progression, metastasis, and survival...
  61. Integrin Beta 4 in Vascular Inflammatory Responses
    Jeffrey R Jacobson; Fiscal Year: 2013
    ..of simvastatin-mediated EC gene expression identified the dramatic upregulation of integrin [unreadable]4, a laminin receptor expressed in numerous cell types including EC, suggesting a potentially novel ALI target...
  62. BASEMENT MEMBRANE SELF-ASSEMBLY AND STRUCTURE
    PETER DANA YURCHENCO; Fiscal Year: 2012
    Basement membranes (BMs) are cell-associated extracellular matrices formed initially as laminin Dolymer scaffoldings...
  63. Intravenous Protein Therapy for the Treatment of Duchenne Muscular Dystrophy
    BRADLEY HODGES; Fiscal Year: 2009
    ..Dean J. Burkin has generated preliminary data demonstrating that a single intramuscular and systemic dose of a laminin isoform to mdx mice, the mouse model of DMD, distributed to all skeletal and cardiac muscles, remained localized ..
  64. Molecular Analysis of Trypanosome Infection
    Fernando Villalta; Fiscal Year: 2010
    ..The mechanism underlying the novel gp83-LOX-1 signal transduction pathway leading to regulation of laminin ?-1 expression and infection will be elucidated using LOX-1(-/-) cells and LOX(+/+) cells...
  65. STRUCTURAL AND MUTATIONAL ANALYSIS OF THE LAMA3 GENE
    Angela Christiano; Fiscal Year: 1999
    ..b>Laminin 5 is expressed in the basement membrane zone of specialized epithelia with secretory or protective functions, ..
  66. MECHANISM OF PC12 CELL ADHESION TO COLLAGEN AND LAMININ
    David Turner; Fiscal Year: 1991
    ..and characterize the cell-surface molecules that mediate neuronal adhesion to two ECM components, collagen and laminin, and to understand the mechanism of this adhesion and its function in development...
  67. ABNORMAL TERMINAL COLON OF THE 1S/1S MUTANT MOUSE
    TAUBE ROTHMAN; Fiscal Year: 2000
    ..In the ls/ls mouse the terminal colon is aganglionic and extracellular matrix molecules including laminin are overexpressed in the colon...
  68. LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
    Karol Bomsztyk; Fiscal Year: 2002
    ..The LAMC1 gene encodes laminin gamma1 (formerly laminin B2) that is a component of heterotrimeric laminin, a molecule that is present in normal ..
  69. Communication Between Laminin 5 and Gap Junctions
    William Carter; Fiscal Year: 2005
    ..Quiescent keratinocytes anchor to laminin 5 in the basement membrane (BM) via integrin a6134 in hemidesmosomes (HD) and integrin alpha-3beta-1 in focal ..
  70. Surface Modified Nanofibers for Nerve Regeneration
    JOSEPH COREY; Fiscal Year: 2009
    ..or coated with extracellular matrix proteins (ECM) or growth factors that promote neurite outgrowth, including laminin, regarded as the most potent substrate-bound promoter of neurite outgrowth...
  71. Laminin-based protein therapeutics for junctional epidermolysis bullosa
    Vitali Alexeev; Fiscal Year: 2009
    ..It is often caused by dysfunction of a connective tissue protein laminin 332, which in turn, results from mutations in LAMA3, LAMB3 or LAMC2 genes encoding 13, 23, and 32 subunits of ..
  72. CONTRACTILE PROTEIN ACCUMULATION IN AIRWAY SMOOTH MUSCLE
    Julian Solway; Fiscal Year: 2002
    ..SA number 3. Identify the role of laminin secretion and binding in regulating contractile apparatus accumulation in airway smooth muscle...
  73. Matrix Control of Glial/Axonal Interactions in Developing Nerves
    Bruce Patton; Fiscal Year: 2009
    ..Contrary to previous assumptions, however, we found they do not act by promoting basal lamina formation. Rather, laminin-2 and -8 each contributes a critical signaling activity, which together promote Schwann cell proliferation and ..