avidin

Summary

Summary: A specific protein in egg albumin that interacts with BIOTIN to render it unavailable to mammals, thereby producing biotin deficiency.

Top Publications

  1. ncbi Tamavidins--novel avidin-like biotin-binding proteins from the Tamogitake mushroom
    Yoshimitsu Takakura
    Plant Innovation Center, Japan Tobacco, Inc, Iwata, Shizuoka, Japan
    FEBS J 276:1383-97. 2009
  2. ncbi Design and construction of highly stable, protease-resistant chimeric avidins
    Vesa P Hytönen
    Department of Biological and Environmental Science, P O Box 35 YAB FIN 40014, University of Jyvaskyla, Finland
    J Biol Chem 280:10228-33. 2005
  3. ncbi Chimeric avidin shows stability against harsh chemical conditions--biochemical analysis and 3D structure
    Juha A E Määttä
    Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland
    Biotechnol Bioeng 108:481-90. 2011
  4. ncbi Biodegradable nanoparticles for direct or two-step tumor immunotargeting
    Leila Nobs
    Department of Pharmaceutical Technology and Biopharmaceutics, School of Pharmaceutical Sciences, Ecole de Pharmacie Genève Lausanne, University of Geneva, Quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland
    Bioconjug Chem 17:139-45. 2006
  5. pmc Three-dimensional structures of avidin and the avidin-biotin complex
    O Livnah
    Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
    Proc Natl Acad Sci U S A 90:5076-80. 1993
  6. ncbi Genetically engineered avidins and streptavidins
    O H Laitinen
    A I Virtanen Institute, Department of Molecular Medicine, University of Kuopio, P O Box 1627, 70211, Kuopio, Finland
    Cell Mol Life Sci 63:2992-3017. 2006
  7. ncbi Sodium dodecyl sulfate-polyacrylamide gel electrophoretic method for assessing the quaternary state and comparative thermostability of avidin and streptavidin
    E A Bayer
    Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel
    Electrophoresis 17:1319-24. 1996
  8. ncbi Biotin induces tetramerization of a recombinant monomeric avidin. A model for protein-protein interactions
    O H Laitinen
    Department of Biological and Environmental Science, , FIN-40351, , Finland
    J Biol Chem 276:8219-24. 2001
  9. ncbi Reversibility of the anti-FXa activity of idrabiotaparinux (biotinylated idraparinux) by intravenous avidin infusion
    I Paty
    Sanofi Aventis Recherche and Développement, Clinical and Pharmacology Department, Chilly Mazarin, France
    J Thromb Haemost 8:722-9. 2010
  10. pmc Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein
    Tiina A Riihimäki
    Institute of Biomedical Technology, University of Tampere and Tampere University Hospital, FI 33520 Tampere, Finland
    BMC Biotechnol 11:64. 2011

Detail Information

Publications291 found, 100 shown here

  1. ncbi Tamavidins--novel avidin-like biotin-binding proteins from the Tamogitake mushroom
    Yoshimitsu Takakura
    Plant Innovation Center, Japan Tobacco, Inc, Iwata, Shizuoka, Japan
    FEBS J 276:1383-97. 2009
    ..These are the first avidin-like proteins to be discovered in organisms other than birds and bacteria...
  2. ncbi Design and construction of highly stable, protease-resistant chimeric avidins
    Vesa P Hytönen
    Department of Biological and Environmental Science, P O Box 35 YAB FIN 40014, University of Jyvaskyla, Finland
    J Biol Chem 280:10228-33. 2005
    The chicken avidin gene family consists of avidin and seven separate avidin-related genes (AVRs) 1-7...
  3. ncbi Chimeric avidin shows stability against harsh chemical conditions--biochemical analysis and 3D structure
    Juha A E Määttä
    Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland
    Biotechnol Bioeng 108:481-90. 2011
    b>Avidin and its bacterial analog streptavidin have been widely used in applications in life sciences...
  4. ncbi Biodegradable nanoparticles for direct or two-step tumor immunotargeting
    Leila Nobs
    Department of Pharmaceutical Technology and Biopharmaceutics, School of Pharmaceutical Sciences, Ecole de Pharmacie Genève Lausanne, University of Geneva, Quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland
    Bioconjug Chem 17:139-45. 2006
    ..have been studied, a direct method using antibody-labeled NPs (mAb-NPs) and a pretargeting method using the avidin-biotin technology...
  5. pmc Three-dimensional structures of avidin and the avidin-biotin complex
    O Livnah
    Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
    Proc Natl Acad Sci U S A 90:5076-80. 1993
    The crystal structures of a deglycosylated form of the egg-white glycoprotein avidin and of its complex with biotin have been determined to 2.6 and 3.0 A, respectively...
  6. ncbi Genetically engineered avidins and streptavidins
    O H Laitinen
    A I Virtanen Institute, Department of Molecular Medicine, University of Kuopio, P O Box 1627, 70211, Kuopio, Finland
    Cell Mol Life Sci 63:2992-3017. 2006
    Chicken avidin and bacterial streptavidin, (strept)avidin, are proteins widely utilized in a number of applications in life science, ranging from purification and labeling techniques to diagnostics, and from targeted drug delivery to ..
  7. ncbi Sodium dodecyl sulfate-polyacrylamide gel electrophoretic method for assessing the quaternary state and comparative thermostability of avidin and streptavidin
    E A Bayer
    Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel
    Electrophoresis 17:1319-24. 1996
    b>Avidin, a positively charged egg-white protein, aggregates extensively when mixed at ambient temperatures with anionic detergents, such as sodium dodecyl sulfate (SDS)...
  8. ncbi Biotin induces tetramerization of a recombinant monomeric avidin. A model for protein-protein interactions
    O H Laitinen
    Department of Biological and Environmental Science, , FIN-40351, , Finland
    J Biol Chem 276:8219-24. 2001
    Chicken avidin, a homotetramer that binds four molecules of biotin was converted to a monomeric form by successive mutations of interface residues to alanine...
  9. ncbi Reversibility of the anti-FXa activity of idrabiotaparinux (biotinylated idraparinux) by intravenous avidin infusion
    I Paty
    Sanofi Aventis Recherche and Développement, Clinical and Pharmacology Department, Chilly Mazarin, France
    J Thromb Haemost 8:722-9. 2010
    Idraparinux is an inhibitor of activated factor X (FXa) with a long half-life allowing once-weekly dosing. Idrabiotaparinux is a biotinylated version of idraparinux; its activity can be reversed with avidin.
  10. pmc Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein
    Tiina A Riihimäki
    Institute of Biomedical Technology, University of Tampere and Tampere University Hospital, FI 33520 Tampere, Finland
    BMC Biotechnol 11:64. 2011
    ..When compared to antibodies, tailored proteins may provide advantageous properties such as a smaller size or a more stable structure...
  11. ncbi Avidin/biotin-liposome system injected in the pleural space for drug delivery to mediastinal lymph nodes
    Luis A Medina
    Department of Radiology, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229 3900, USA
    J Pharm Sci 93:2595-608. 2004
    ..Nodal uptake was determined after intrapleural injection of the avidin/biotin-liposome system in normal rats...
  12. ncbi Avidin functionalized maghemite nanoparticles and their application for recombinant human biotinyl-SERCA purification
    Massimiliano Magro
    Department of Comparative Biomedicine and Food Science, University of Padua, Italy
    Langmuir 28:15392-401. 2012
    ..one-step, green procedure and functionalized with ligands of high biotechnological interest, such as biotin and avidin, by simple incubation in aqueous solution...
  13. ncbi Protein supramolecular complex formation by site-specific avidin-biotin interactions
    Yutaro Mori
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819 0395, Japan
    Org Biomol Chem 11:914-22. 2013
    ..The avidin-biotin interaction is widely employed in the design of functional protein self-assemblies...
  14. ncbi (Strept)avidin-displaying lentiviruses as versatile tools for targeting and dual imaging of gene delivery
    M U Kaikkonen
    Department of Biotechnology and Molecular Medicine, AI Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland
    Gene Ther 16:894-904. 2009
    ..We have developed a dual-imaging strategy based on avidin-biotin system allowing easy exchange of the surface ligand on HIV-derived lentivirus envelope...
  15. pmc Efficient cytosolic delivery of molecular beacon conjugates and flow cytometric analysis of target RNA
    Antony K Chen
    Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    Nucleic Acids Res 36:e69. 2008
    ..Overall, the present study introduces an improved methodology for the high-throughput detection of RNA at the single cell level...
  16. ncbi Use of avidin/biotin-liposome system for enhanced peritoneal drug delivery in an ovarian cancer model
    Cristina L Zavaleta
    Department of Radiology, MSC 7800, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229 3900, USA
    Int J Pharm 337:316-28. 2007
    The goal of this study was to determine the distribution of the avidin/biotin-liposome system in an ovarian cancer xenograft model...
  17. ncbi Avidin-biotin technology in targeted therapy
    Hanna P Lesch
    University of Eastern Finland, A I Virtanen Institute, Department of Biotechnology and Molecular Medicine, Kuopio, Finland
    Expert Opin Drug Deliv 7:551-64. 2010
    ..Many approaches in cancer treatment are limited because of their broad range of unwanted side effects on healthy cells. Targeting can reduce side effects and increase efficacy of drugs in the patient...
  18. ncbi Hepatocyte targeting of 111In-labeled oligo-DNA with avidin or avidin-dendrimer complex
    Marcelo Mamede
    Department of Nuclear Medicine and Diagnostic Imaging, Graduate School of Medicine, Kyoto University, 54 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    J Control Release 95:133-41. 2004
    ..gene transfer vector to hepatocytes, various oligo-carrier complexes were developed employing dendrimer (G4) and avidin-biotin systems (Av-bt), and their biodistribution were evaluated...
  19. ncbi Pretargeted radioimmunotherapy of cancer: progress step by step
    Otto C Boerman
    Department of Nuclear Medicine, University Medical Center Nijmegen, Nijmegen, The Netherlands
    J Nucl Med 44:400-11. 2003
    ..can be distinguished: pretargeting strategies based on the avid interaction between streptavidin (SA) or avidin and biotin, and pretargeting strategies based on the use of bispecific antibodies...
  20. ncbi Accurate titration of avidin and streptavidin with biotin-fluorophore conjugates in complex, colored biofluids
    H J Gruber
    Institute of Biophysics, J Kepler University, Altenberger Str 69, A 4040 Linz, Austria
    Biochim Biophys Acta 1381:203-12. 1998
    A new fluorimetric assay is presented for the specific and reliable quantitation of >/=2 nM avidin and streptavidin...
  21. pmc Bifunctional avidin with covalently modifiable ligand binding site
    Jenni Leppiniemi
    Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland
    PLoS ONE 6:e16576. 2011
    The extensive use of avidin and streptavidin in life sciences originates from the extraordinary tight biotin-binding affinity of these tetrameric proteins...
  22. ncbi Controlling quaternary structure assembly: subunit interface engineering and crystal structure of dual chain avidin
    Vesa P Hytönen
    Nanoscience Center, Department of Biological and Environmental Science, University of Jyvaskyla, Finland
    J Mol Biol 359:1352-63. 2006
    Dual chain avidin (dcAvd) is an engineered avidin form, in which two circularly permuted chicken avidin monomers are fused into one polypeptide chain...
  23. ncbi Novel avidin-like protein from a root nodule symbiotic bacterium, Bradyrhizobium japonicum
    Henri R Nordlund
    Department of Biological and Environmental Science, Nanoscience Center, P O Box 35 YAB, FIN 40014 University of Jyväskylä, Finland
    J Biol Chem 280:13250-5. 2005
    ..These bacteria have a gene encoding a putative avidin- and streptavidin-like protein, which bears an amino acid sequence identity of only about 30% over the core ..
  24. pmc Avidin related protein 2 shows unique structural and functional features among the avidin protein family
    Vesa P Hytönen
    Nanoscience Center, Department of Biological and Environmental Science, University of Jyvaskyla, P O Box 35 YAB, FI 40014, Finland
    BMC Biotechnol 5:28. 2005
    The chicken avidin gene family consists of avidin and several avidin related genes (AVRs)...
  25. pmc Rhizavidin from Rhizobium etli: the first natural dimer in the avidin protein family
    Satu H Helppolainen
    Institute of Medical Technology, University of Tampere, FI 33014 Tampere, Finland
    Biochem J 405:397-405. 2007
    ..The symbiotic plasmid p42d of R. etli comprises a gene encoding a putative (strept)avidin-like protein, named rhizavidin...
  26. ncbi Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation
    James D Hirsch
    Molecular Probes, Inc, Eugene, OR 97402, USA
    Anal Biochem 308:343-57. 2002
    The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades...
  27. ncbi Avidin: a natural bridge for quantum dot-antibody conjugates
    Ellen R Goldman
    U S Naval Research Laboratory, Center for Bio Molecular Science and Engineering and Division of Optical Sciences, Washington, D C 20375, USA
    J Am Chem Soc 124:6378-82. 2002
    ..semiconductor CdSe-ZnS core-shell nanocrystal quantum dots (QDs) were coupled to antibodies through the use of an avidin bridge adsorbed to the nanocrystal surface via electrostatic self-assembly...
  28. ncbi Rational modification of ligand-binding preference of avidin by circular permutation and mutagenesis
    Juha A E Määttä
    Institute of Medical Technology, University of Tampere, 33014 University of Tampere, Finland
    Chembiochem 9:1124-35. 2008
    Chicken avidin is a key component used in a wide variety of biotechnological applications. Here we present a circularly permuted avidin (cpAvd4-->3) that lacks the loop between beta-strands 3 and 4...
  29. ncbi Bradavidin II from Bradyrhizobium japonicum: a new avidin-like biotin-binding protein
    Satu H Helppolainen
    Institute of Medical Technology, Biokatu 6, FI 33014 University of Tampere and Tampere University Hospital, Finland
    Biochim Biophys Acta 1784:1002-10. 2008
    A gene encoding an avidin-like protein was discovered in the genome of B. japonicum. The gene was cloned to an expression vector and a protein, named bradavidin II, was produced in E. coli...
  30. pmc The lipocalin protein family: structure and function
    D R Flower
    Department of Physical Chemistry and BioAnalysis, Astra Charnwood, Loughborough, Leics, U K
    Biochem J 318:1-14. 1996
    ..The lipocalins have also been implicated in the regulation of cell homoeostasis and the modulation of the immune response, and, as carrier proteins, to act in the general clearance of endogenous and exogenous compounds...
  31. ncbi A quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris
    Carmen Jungo
    Ecole Polytechnique Federale de Lausanne, Laboratoire de Génie Chimique et Biologique, Station 6, Lausanne, Switzerland
    J Biotechnol 131:57-66. 2007
    ..pastoris Mut(+) strain secreting avidin by performing a transient nutrient gradient in continuous cultivation at a dilution rate of 0.03h(-1)...
  32. pmc Structural and functional characteristics of xenavidin, the first frog avidin from Xenopus tropicalis
    Juha A E Määttä
    Institute of Medical Technology, Biokatu 6, FI 33014 University of Tampere and Tampere University Hospital, Tampere, Finland
    BMC Struct Biol 9:63. 2009
    ..Here we structurally and functionally characterise a novel avidin named xenavidin, which is to our knowledge the first reported avidin from a frog.
  33. ncbi Molecular cloning and nucleotide sequence of chicken avidin-related genes 1-5
    R A Keinanen
    Department of Biomedical Sciences, University of Tampere, Finland
    Eur J Biochem 220:615-21. 1994
    Using avidin cDNA as a hybridisation probe, we detected a gene family whose putative products are related to the chicken egg-white avidin...
  34. ncbi Dynamic force measurements of avidin-biotin and streptavdin-biotin interactions using AFM
    Marzena de Odrowaz Piramowicz
    Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland
    Acta Biochim Pol 53:93-100. 2006
    ..atomic force microscopy (AFM) we performed dynamic force measurements of the adhesive forces in two model systems: avidin-biotin and streptavidin-biotin...
  35. pmc Characterization of poultry egg-white avidins and their potential as a tool in pretargeting cancer treatment
    Vesa P Hytönen
    Department of Biological and Environmental Science, University of Jyvaskyla, P O Box 35 YAB, Jyvaskyla, FIN 40014, Finland
    Biochem J 372:219-25. 2003
    Chicken avidin and bacterial streptavidin are proteins used in a wide variety of applications in the life sciences due to their strong affinity for biotin. A new and promising use for them is in medical pretargeting cancer treatments...
  36. ncbi Critical importance of loop conformation to avidin-enhanced hydrolysis of an active biotin ester
    Ruchama Hayouka
    Department of Biological Chemistry, The Institute of Life Sciences, The Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
    Acta Crystallogr D Biol Crystallogr 64:302-8. 2008
    The homotetrameric and biotin-binding properties of avidin and streptavidin have been exploited for a myriad of biotechnological applications and theoretical studies...
  37. ncbi Artificial metalloenzymes based on the biotin-avidin technology: enantioselective catalysis and beyond
    Thomas R Ward
    Department of Chemistry, University of Basel, Spitalstrasse 51, CH 4056 Basel, Switzerland
    Acc Chem Res 44:47-57. 2011
    ..Inspired by a visionary report by Wilson and Whitesides in 1978, we have exploited the potential of biotin-avidin technology in creating artificial metalloenzymes...
  38. ncbi Structure-based rational design of streptavidin mutants with pseudo-catalytic activity
    Yael Pazy
    Department of Biological Chemistry, The Institute of Life Sciences, The Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
    J Biol Chem 278:7131-4. 2003
    ..study, we identify the structural elements that contribute to the observed hydrolytic activity in egg white avidin, which promote the cleavage of active biotin esters (notably biotinyl p-nitrophenyl ester)...
  39. ncbi Therapeutic use of avidin is not hampered by antiavidin antibodies in humans
    Fiorella Petronzelli
    R and D Immunology, Sigma Tau SpA, Pomezia, Rome, Italy
    Cancer Biother Radiopharm 25:563-70. 2010
    Hen egg white avidin is increasingly used in the clinic as part of multifactor treatments such as pretargeted radionuclide therapy of cancer or as an antidote of biotinylated drugs...
  40. ncbi Mediastinal node and diaphragmatic targeting after intracavitary injection of avidin/99mTc-blue-biotin-liposome system
    Luis A Medina
    Instituto de Fisica, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico
    J Pharm Sci 95:207-24. 2006
    ..Mediastinal node and lymphatic distributions were determined after intracavitary injection of the avidin/biotin-liposome system in normal rats...
  41. pmc Zebavidin--an avidin-like protein from zebrafish
    Barbara Taskinen
    Institute of Biomedical Technology, University of Tampere, BioMediTech, Tampere, Finland Fimlab Laboratories, Pirkanmaa Hospital District, Tampere, Finland
    PLoS ONE 8:e77207. 2013
    The avidin protein family members are well known for their high affinity towards D-biotin and high structural stability. These properties make avidins valuable tools for a wide range of biotechnology applications...
  42. ncbi Conformational flexibility of avidin: the influence of biotin binding
    M Soledad Celej
    Centro de Investigaciones en Química Biológica de Córdoba CIQUIBIC, Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina
    Biochem Biophys Res Commun 325:922-7. 2004
    ..In this work, we have explored the effect of biotin binding on conformation and dynamic properties of avidin by using infrared spectroscopy including kinetics of hydrogen/deuterium exchange...
  43. ncbi Biotinylated disulfide containing PEI/avidin bioconjugate shows specific enhanced transfection efficiency in HepG2 cells
    Xuan Zeng
    Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan, 430072, P R China
    Org Biomol Chem 7:4201-10. 2009
    ..The obtained biotinylated PEI-SS was bioconjugated with avidin via the biotin-avidin interaction to form a novel gene vector, biotinylated PEI-SS/avidin bioconjugate (ABP-SS)...
  44. ncbi Protein assemblies by site-specific avidin-biotin interactions
    Yutaro Mori
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan
    Org Biomol Chem 9:5641-4. 2011
    ....
  45. pmc Chicken genome analysis reveals novel genes encoding biotin-binding proteins related to avidin family
    Einari A Niskanen
    Nanoscience Center, Department of Biological and Environmental Science, FIN 40014 University of Jyväskylä, Finland
    BMC Genomics 6:41. 2005
    ....
  46. ncbi Biotin-avidin interaction-based screening assay for Alzheimer's beta-peptide oligomer inhibitors
    Harry LeVine
    Department of Molecular and Cellular Biochemistry, Chandler School of Medicine, and Center on Aging, University of Kentucky, Lexington, KY 40536, USA
    Anal Biochem 356:265-72. 2006
    ..concentrations of Alzheimer's beta-peptide residues 1-42 [Abeta(1-42)] oligomers incorporating the biotin-avidin interaction that has been a workhorse for screening assays is applied here in a single-site NeutrAvidin capture/..
  47. ncbi Avidin-biotin binding-based cell seeding and perfusion culture of liver-derived cells in a porous scaffold with a three-dimensional interconnected flow-channel network
    Hongyun Huang
    Center for Disease Biology and Integrative Medicine CDBIM, Gradate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    Biomaterials 28:3815-23. 2007
    ..To evaluate its biocompatibility, human hepatoma Hep G2 cells were seeded into the scaffold using avidin-biotin (AB) binding and cultured in a perfusion system for 9 days...
  48. ncbi Covalent biofunctionalization of cellulose acetate with thermostable chimeric avidin
    Jarkko J Heikkinen
    Department of Chemistry, University of Oulu, P O Box 3000, FI 90014 Oulu, Finland
    ACS Appl Mater Interfaces 3:2240-5. 2011
    ..bioactive cellulose acetate (CA) surface was developed by functionalizing the surface with highly thermostable avidin form...
  49. pmc Chemical linkage to injected tissues is a distinctive property of oxidized avidin
    Rita De Santis
    Department of Immunology, Sigma Tau SpA, Pomezia, Rome, Italy
    PLoS ONE 6:e21075. 2011
    We recently reported that the oxidized avidin, named AvidinOX®, resides for weeks within injected tissues as a consequence of the formation of Schiff's bases between its aldehyde groups and tissue protein amino groups...
  50. ncbi Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates
    Jarkko J Heikkinen
    Department of Chemistry, University of Oulu, Oulu, Finland
    Colloids Surf B Biointerfaces 87:409-14. 2011
    A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol-gel substrates was developed...
  51. ncbi Antibody fragments in tumor pretargeting. Evaluation of biotinylated Fab' colocalization with recombinant streptavidin and avidin
    D S Wilbur
    Department of Radiation Oncology, University of Washington, Seattle 98195, USA
    Bioconjug Chem 7:689-702. 1996
    ..As a model system, tumor colocalization of avidin or recombinant streptavidin (r-streptavidin) and the biotinylated Fab' fragment (Fab'-S-biotin) of A6H, an ..
  52. pmc Delivery of a peptide radiopharmaceutical to brain with an IgG-avidin fusion protein
    Qing Hui Zhou
    Department of Medicine, University of California at Los Angeles, California 90024, United States
    Bioconjug Chem 22:1611-8. 2011
    ..b>Avidin (AV) is fused to the carboxyl terminus of the heavy chain of the genetically engineered chimeric monoclonal ..
  53. pmc Quantitative label-free characterization of avidin-biotin assemblies on silanized glass
    Li Jung Chen
    Department of Chemistry, Texas A and M University, College Station, Texas, United States
    Anal Chem 83:7173-8. 2011
    ..mass spectrometer TOF-SIMS, operating in the event-by-event bombardment/detection mode was used to characterize avidin-biotin assemblies on silane-modified glass substrates...
  54. ncbi Comparison of the energetics of avidin, streptavidin, neutrAvidin, and anti-biotin antibody binding to biotinylated lipid bilayer examined by second-harmonic generation
    Trang T Nguyen
    Department of Chemistry, University of Utah, 315 South 1400 East, RM 2020, Salt Lake City, Utah 84112, USA
    Anal Chem 84:201-8. 2012
    A comparison of the binding properties of avidin, streptavidin, neutrAvidin, and antibiotin antibody to a biotinylated lipid bilayer was studied using second-harmonic generation...
  55. ncbi Pretargeting of necrotic tumors with biotinylated hypericin using 123I-labeled avidin: evaluation of a two-step strategy
    Thierry Marysael
    Laboratorium voor Farmaceutische Biologie, Faculteit Farmaceutische Wetenschappen, KU Leuven, Herestraat 49 bus 824, B 3000, Leuven, Belgium
    Invest New Drugs 30:2132-40. 2012
    ..With this conjugate, we explored the possibility of a two-step pretargeting strategy using (123)I-labeled avidin as effector molecule directed against necrotic RIF-1 tumors...
  56. ncbi Resonance assignments of the 56 kDa chimeric avidin in the biotin-bound and free forms
    Helena Tossavainen
    Program in Structural Biology and Biophysics, Institute of Biotechnology, Viikinkaari 1, P O Box 65, 00014, Helsinki, Finland
    Biomol NMR Assign 7:35-8. 2013
    b>Avidin is a homotetrameric ~56 kDa protein found in chicken egg white. Avidin's ability to bind biotin with a very high affinity has widely been exploited in biotechnological applications...
  57. ncbi Monolithic columns with immobilized monomeric avidin: preparation and application for affinity chromatography
    Jens Spross
    Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    Anal Bioanal Chem 402:2395-405. 2012
    ..dimethacrylate) monolith were prepared in fused silica capillaries (100 μm ID) and modified with monomeric avidin using the glutaraldehyde technique...
  58. ncbi Systematic evaluation of avidin-biotin interaction by fluorescence spectrophotometry
    Shuguo Sun
    National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China
    Spectrochim Acta A Mol Biomol Spectrosc 89:99-104. 2012
    The avidin-biotin interaction was evaluated systematically by fluorescence spectroscopy under different conditions of temperature, pressure, pH, metal ions, incubation time and initial avidin concentration...
  59. ncbi Enhancing the thermal stability of avidin. Introduction of disulfide bridges between subunit interfaces
    Henri R Nordlund
    Department of Biological and Environmental Science, P O Box 35 YAB, FIN 40014 University of Jyväskylä, Finland
    J Biol Chem 278:2479-83. 2003
    In this study we showed that tetrameric chicken avidin can be stabilized by introducing intermonomeric disulfide bridges between its subunits...
  60. ncbi Preclinical pharmacology and safety of a novel avidin derivative for tissue-targeted delivery of radiolabelled biotin
    Fiorella Petronzelli
    Sigma Tau SpA R and D, Pomezia, Rome, Italy
    Basic Clin Pharmacol Toxicol 109:145-55. 2011
    We recently described an oxidized avidin variant, named AvidinOX(®) , which is a product that chemically links to tissue proteins while maintaining the capacity to uptake intravenously administered biotin...
  61. ncbi Insecticidal activity of recombinant avidin produced in yeast
    Gareth Hinchliffe
    School of Biological and Biomedical Sciences, University of Durham, Science Laboratories, South Road, Durham DH1 3LE, UK
    J Insect Physiol 56:629-39. 2010
    An expression construct encoding chicken (Gallus gallus) avidin was assembled from amplified fragments of genomic DNA. Recombinant, functional avidin was produced in Pichia pastoris, with yields of up to 80 mg/l of culture supernatant...
  62. ncbi Endogenous biotin in rat brain: implications for false-positive results with avidin-biotin and streptavidin-biotin techniques
    Bruce E McKay
    Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada
    Methods Mol Biol 418:111-28. 2008
    The interaction between avidin and biotin or streptavidin and biotin forms the basis of several widely used immunohistochemical techniques...
  63. ncbi Direct immobilization of avidin protein on AFM tip functionalized by acrylic acid vapor at RF plasma
    Lilian T Costa
    Instituto de Biofisica, Universidade Federal do Rio de Janeiro Polo Xerém, Duque de Caxias 25245 390, Brazil
    J Mol Recognit 25:256-61. 2012
    ..The Fourier transform-infrared spectroscopy spectrum shows a high absorbance of avidin protein over the silicon surface functionalized by AA plasma treatment...
  64. ncbi Label free optical sensor for Avidin based on single gold nanoparticles functionalized with aptamers
    Frank Jeyson Hernandez
    Department of Chemical Engineering, Universitat Rovira i Virgili, Tarragona, Spain
    J Biophotonics 2:227-31. 2009
    ..of a single gold nanoparticle, functionalized with an aptamer, is used to sense the specific binding of avidin. Herewith, the field of single noble metal nanoparticle biosensors is extended to the important field of aptamer ..
  65. ncbi Avidin is a heparin-binding protein. Affinity, specificity and structural analysis
    Warren C Kett
    Molecular Immunology Laboratory, School of Biomedical Sciences, Curtin University of Technology, Level 5 MRF Building, Rear 50 Murray Street, Perth, Western Australia 6000, Australia
    Biochim Biophys Acta 1620:225-34. 2003
    The specificity, affinity and stoichiometry of the interaction between avidin and glycosaminoglycans (GAGs) have been investigated using heparin-coated microtiter-plate assays, a filter binding assay and surface plasmon resonance (SPR) ..
  66. ncbi A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate
    Yukihiro Hama
    Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1088, USA
    Cancer Res 67:2791-9. 2007
    ..quot; In this study, an alternate fluorescence activation strategy is proposed whereby self-quenching avidin-rhodamine X, which has affinity for lectin on cancer cells, is activated after endocytosis and degradation within ..
  67. ncbi Nucleic acid intercalators and avidin probes derived from luminescent cyclometalated iridium(III)-dipyridoquinoxaline and -dipyridophenazine complexes
    Kenneth Kam Wing Lo
    Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, People s Republic of China
    Chemistry 12:1500-12. 2006
    ..dppzB (6)) have been designed as luminescent intercalators for DNA and probes for avidin. The structure of complex 4 has been studied by X-ray crystallography...
  68. ncbi Target visualization by MRI using the avidin/biotin amplification route: synthesis and testing of a biotin-Gd-DOTA monoamide trimer
    Lorenzo Tei
    Dipartimento di Scienze dell Ambiente e della Vita, Universita del Piemonte Orientale Amedeo Avogadro, Viale T Michel 11, 15121 Alessandria, Italy
    Chemistry 16:8080-7. 2010
    ..in magnetic resonance (MR) molecular imaging applications, the formation of supramolecular adducts between (strept)avidin ((S)Av) and tribiotinylated Gd-DOTA-monoamide complexes (DOTA=1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-..
  69. pmc A synthetic host-guest system achieves avidin-biotin affinity by overcoming enthalpy-entropy compensation
    Mikhail V Rekharsky
    ICORP Entropy Control Project JST and Department of Applied Chemistry, Osaka University, Yamada oka, Suita 565 0871, Japan
    Proc Natl Acad Sci U S A 104:20737-42. 2007
    ..constant for this host-guest pair is 3 x 10(15) M(-1) (K(d) = 3 x 10(-16) M), equivalent to that exhibited by the avidin-biotin pair...
  70. ncbi Biological and chemical decoration of peptide nanostructures via biotin-avidin interactions
    Meital Reches
    Department of Molecular Microbiology and Biotechnology, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    J Nanosci Nanotechnol 7:2239-45. 2007
    ..chemical modification of ADNT with biotin moieties, in order to enable the selective decoration of the tubes with avidin-labeled species. First, ADNT were prepared in aqueous solution by self-assembly of the dipeptide building blocks...
  71. ncbi Polymerase chain reaction coupling with magnetic nanoparticles-based biotin-avidin system for amplification of chemiluminescent detection signals of nucleic acid
    Zhiyang Li
    State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
    J Nanosci Nanotechnol 11:1074-8. 2011
    ....
  72. ncbi A human biotin acceptor domain allows site-specific conjugation of an enzyme to an antibody-avidin fusion protein for targeted drug delivery
    Tsuneaki Asai
    Department of Microbiology, Molecular Genetics and Molecular Biology Institute, University of California Los Angeles, CA 90095, USA
    Biomol Eng 21:145-55. 2005
    We have previously constructed an antibody-avidin (Av) fusion protein, anti-transferrin receptor (TfR) IgG3-Av, which can deliver biotinylated molecules to cells expressing the TfR...
  73. ncbi Avidin expression during chick chondrocyte and myoblast development in vitro and in vivo: regulation of cell proliferation
    B Zerega
    Istituto Nazionale per la Ricerca sul Cancro, Centro Biotecnologie Avanzate, Genova, Italy
    J Cell Sci 114:1473-82. 2001
    b>Avidin is a major [35S]methionine-labeled protein induced by bacterial lipopolysaccharide (LPS) and interleukin 6 (IL-6) in cultured chick embryo myoblasts and chondrocytes...
  74. ncbi The avidin-biotin complex (ABC) method and other avidin-biotin binding methods
    Gary L Bratthauer
    Department of Gynecologic and Breast Pathology, Armed Forces Institute of Pathology, Washington, DC, USA
    Methods Mol Biol 588:257-70. 2010
    Immunoenzyme methods can be enhanced by the use of the high affinity molecules, avidin and biotin. The binding of avidin to biotin is almost irreversible...
  75. ncbi Probing the interaction of the biotin-avidin complex with the relaxivity of biotinylated Gd-DTPA
    Sander Langereis
    Laboratory of Macromolecular and Organic Chemistry, Eindhoven University of Technology, P O Box 513, 5600 MB, Eindhoven, The Netherlands
    Org Biomol Chem 2:1271-3. 2004
    A biotinylated Gd-DTPA complex is designed to study the biotin-avidin complexation using the longitudinal relaxivity of this new MRI label, which illustrates the use of MRI contrast agents to probe the formation of supramolecular ..
  76. ncbi Three-step tumor targeting of paclitaxel using biotinylated PLA-PEG nanoparticles and avidin-biotin technology: Formulation development and in vitro anticancer activity
    Mika Pulkkinen
    Department of Pharmaceutics, University of Kuopio, Kuopio, Finland
    Eur J Pharm Biopharm 70:66-74. 2008
    ..In this study, three-step tumor targeting of paclitaxel using biotinylated PLA-PEG nanoparticles and avidin-biotin technology was evaluated in vitro as a way of enhancing delivery of paclitaxel...
  77. ncbi Recording intracellular molecular events from the outside: glycosylphosphatidylinositol-anchored avidin as a reporter protein for in vivo imaging
    Steffi Lehmann
    University of Zurich, Zurich, Switzerland
    J Nucl Med 52:445-52. 2011
    ..Here we describe the use of glycosylphosphatidylinositol (GPI)-anchored avidin, an avidin moiety targeted to the extracellular side of cell membranes via a GPI anchor, as a reporter for in vivo ..
  78. pmc Demonstration of gastrin cell autoantibodies in antral gastritis with avidin-biotin complex antibody technique
    R M Uibo
    Clin Exp Immunol 58:341-7. 1984
    In the present study we used the highly sensitive avidin-biotin complex technique (ABC) for detection of gastrin cell (G cell) autoantibodies...
  79. pmc Inhibition of NF-kappaB and Akt pathways by an antibody-avidin fusion protein sensitizes malignant B-cells to cisplatin-induced apoptosis
    Eriko Suzuki
    Department of Urology, School of Medicine, Keio University, Shinjuku ku, Tokyo 165 8582, Japan
    Int J Oncol 36:1299-307. 2010
    ..protein consisting of an IgG3 specific for the human transferrin receptor 1 (TfR1, CD71) genetically fused to avidin at its carboxy-terminus (ch128.1Av). We have previously shown that ch128...
  80. ncbi In situ IR spectroscopic studies of the avidin-biotin bioconjugation reaction on CdS particle films
    Aidan G Young
    Department of Chemistry, University of Otago, Dunedin, New Zealand
    Langmuir 25:7416-23. 2009
    b>Avidin-biotin bioconjugation reactions have been carried out on CdS nanoparticle films in H2O and D2O and investigated using in situ ATR-IR spectroscopic techniques...
  81. ncbi Delivery of NADPH-cytochrome P450 reductase antisense oligos using avidin-biotin approach
    Venkateswaran C Pillai
    Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, 1300 Coulter, Amarillo, Texas 79106, USA
    Bioconjug Chem 21:203-7. 2010
    Although avidin-mediated intracellular delivery of oligonucleotides or proteins has been shown before, the efficacy studies are lacking...
  82. pmc Assembling of G-strands into novel tetra-molecular parallel G4-DNA nanostructures using avidin-biotin recognition
    Natalia Borovok
    Department of Biochemistry, George S Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, 69978
    Nucleic Acids Res 36:5050-60. 2008
    ..To enable a tetra-molecular assembly of the G-strands we developed a novel approach based on avidin-biotin biological recognition...
  83. ncbi Immobilization and surface characterization of NeutrAvidin biotin-binding protein on different hydrogel interlayers
    Patrick Vermette
    CSIRO Molecular Science, Bag 10, Clayton South, Victoria 3169, Australia
    J Colloid Interface Sci 259:13-26. 2003
    For a number of potential applications, it is desirable to immobilize avidin class molecules onto solid supports and exploit their ability to bind biotinylated molecules with high affinity...
  84. ncbi Ultra sensitive affinity chromatography on avidin-functionalized PMMA microchip for low abundant post-translational modified protein enrichment
    Hui Xia
    Department of Biomedical Engineering, Louisiana Tech University, Ruston, LA, USA
    Biomed Microdevices 14:67-81. 2012
    ..In this paper, we report a novel and sensitive affinity chromatography on the avidin-functionalized poly(methyl methacrylate) (PMMA) microchip for enrichment of nanogram (ng) amount of PTMs...
  85. ncbi An SPR immunosensor for human cardiac troponin T using specific binding avidin to biotin at carboxymethyldextran-modified gold chip
    Rosa Fireman Dutra
    Departamento de Patologia, Instituto de Ciencias Biologicas, Universidade de Pernambuco, Recife, Brasil, Rua Arnóbio Marques, 310, Santo Amaro, 50100 130 Recife, PE, Brazil
    Clin Chim Acta 376:114-20. 2007
    ..Surface plasmon resonance (SPR) immunosensor was developed for the quick detection of human cTnT in real time...
  86. ncbi Lectin precipitation using phytohemagglutinin-L(4) coupled to avidin-agarose for serological biomarker discovery in colorectal cancer
    Yong Sam Kim
    Daejeon KRIBB FHCRC Research Cooperation Center, KRIBB, 111 Gwahangno, Yuseong Gu, Deajeon, Korea
    Proteomics 8:3229-35. 2008
    ..serum proteins were subjected to immunodepletion, alkylation, and lectin precipitation using L-PHA coupled to avidin-agarose bead complexes, and tryptic digestion...
  87. ncbi Application of the avidin-biotin interaction to immobilize DNA in the development of electrochemical impedance genosensors
    A Bonanni
    Sensors and Biosensors Group, Department of Chemistry, Autonomous University of Barcelona, Edifici Cn, 08193 Bellaterra, Barcelona, Spain
    Anal Bioanal Chem 389:851-61. 2007
    ..In this work, an avidin bulk-modified graphite-epoxy biocomposite (Av-GEB) was employed to immobilize biotinylated oligonucleotides as ..
  88. pmc Fluorescent avidin-bound silver particle: a strategy for single target molecule detection on a cell membrane
    Jian Zhang
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA
    Anal Chem 81:883-9. 2009
    Cy5-avidin conjugate-bound silver nanoparticles were prepared as a fluorescence molecular reagent for the cell imaging...
  89. ncbi Impedimetric immunosensor using avidin-biotin for antibody immobilization
    O Ouerghi
    Ingénierie et Fonctionnalisation des Surfaces, UMR CNRS 5621, Ecole Centrale de Lyon, BP 163, F 69131 Ecully cedex, France
    Bioelectrochemistry 56:131-3. 2002
    ..IgG), used as a model system, was attached to free biotin groups on the electrogenerated polypyrrole film using avidin as a coupling reagent. This immobilization method allows to obtain a highly reproducible and stable device...
  90. ncbi Tumor targeting and imaging of intraperitoneal tumors by use of antisense oligo-DNA complexed with dendrimers and/or avidin in mice
    N Sato
    Department of Nuclear Medicine and Diagnostic Imaging, Kyoto University, 54 Kawaharacho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan
    Clin Cancer Res 7:3606-12. 2001
    ..111)In-oligo/G4-Bt and (111)In-oligo-Bt were conjugated to avidin ((111)In-oligo/G4-Av and (111)In-oligo-Av, respectively)...
  91. ncbi Active targeting behaviors of biotinylated pluronic/poly(lactic acid) nanoparticles in vitro through three-step biotin-avidin interaction
    Xiang Yuan Xiong
    School of Life Science, Jiangxi Science and Technology Normal University, Nanchang, PR China
    J Biomater Sci Polym Ed 22:1607-19. 2011
    ..properties of B-F87-PLA and B-P85-PLA nanoparticles in vitro were investigated through a three-step biotin-avidin interaction by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) tests and fluorescence ..
  92. ncbi Hidden variety of biotin-streptavidin/avidin local interactions revealed by site-selective dynamic force spectroscopy
    Atsushi Taninaka
    Institute of Applied Physics, CREST JST, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Ibaraki, Japan
    Phys Chem Chem Phys 12:12578-83. 2010
    ..between the local structures of amino acid residues at the middle sites, SER45 and THR35 for streptavidin and avidin, respectively, strongly affects the microscopic reaction processes, i.e...
  93. ncbi Chicken avidin exhibits pseudo-catalytic properties. Biochemical, structural, and electrostatic consequences
    T Huberman
    Department of Biological Chemistry, The Institute of Life Sciences, Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel
    J Biol Chem 276:32031-9. 2001
    b>Avidin and its bacterial analogue streptavidin exhibit similarly high affinities toward the vitamin biotin. The extremely high affinity of these two proteins has been utilized as a powerful tool in many biotechnological applications...
  94. ncbi Hexon-specific PEGylated adenovirus vectors utilizing avidin-biotin interaction
    Emi Suzuki-Kouyama
    Laboratory of Gene Transfer and Regulation, National Institute of Biomedical Innovation, Osaka, Japan
    Biomaterials 32:1724-30. 2011
    ..hexon, which is not involved in the binding to CAR, and PEG was then specifically conjugated to the hexon HVR5 via avidin-biotin interaction...
  95. ncbi Radiosynthesis, biodistribution and micro-SPECT imaging study of dendrimer-avidin conjugate
    Xiaoping Xu
    Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China
    Bioorg Med Chem 19:1643-8. 2011
    ..minepentaacetic acid (1B4M-DTPA), respectively to form the complex Bt-G5-Ac-1B4M which was further conjugated with avidin to give the conjugate Av-G5-Ac-1B4M...
  96. ncbi CD16/32-specific biotinylated 2.4G2 single-chain Fv complexed with avidin-FITC enhances FITC-specific humoral immune response in vivo in a CD16-dependent manner
    Adrienn Angyal
    Department of Immunology, Eotvos Lorand University, 1117 Budapest, Hungary
    Int Immunol 22:71-80. 2010
    ..4G2, linked to avidin-FITC, and tested its effect on the FITC-hapten-specific T-independent type 2 (TI-2) and T-dependent (TD) immune ..
  97. ncbi Radioimmunotherapy: is avidin-biotin pretargeting the preferred choice among pretargeting methods?
    David M Goldenberg
    Center for Molecular Medicine and Immunology, Garden State Cancer Center, 520 Belleville Avenue, NJ 07109 0023, Belleville, USA
    Eur J Nucl Med Mol Imaging 30:777-80. 2003
  98. ncbi DNA family shuffling within the chicken avidin protein family - A shortcut to more powerful protein tools
    Barbara Niederhauser
    Institute of Biomedical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland
    J Biotechnol 157:38-49. 2012
    ..In this study, we show that it is possible to create functional chimeric proteins from the avidin protein family when applying DNA family shuffling to the genes of the avidin protein family: avidin, avidin ..
  99. ncbi Single and multiple bonds in (strept)avidin-biotin interactions
    Jean Marie Teulon
    CEA, IBEB, Service de Biochimie et Toxicologie Nucléaire, F 30207 Bagnols sur Ceze, France
    J Mol Recognit 24:490-502. 2011
    ..We have chosen the well-known system, (strept)avidin-biotin complex, as an experimental model due to the lack of consensus on interpretations of the rupture force ..
  100. ncbi Layer-by-layer construction of protein architectures through avidin-biotin and lectin-sugar interactions for biosensor applications
    Shigehiro Takahashi
    Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Anal Bioanal Chem 402:1749-58. 2012
    ..the preparation and properties of protein architectures constructed by layer-by-layer (LbL) deposition through avidin-biotin and concanavalin A (Con A)-sugar interactions are discussed in relation to their use for optical and ..
  101. ncbi The AviD-tag, a NeutrAvidin/avidin specific peptide affinity tag for the immobilization and purification of recombinant proteins
    Thomas Gaj
    Department of Chemistry, University of Arizona, Tucson, AZ 85721, USA
    Protein Expr Purif 56:54-61. 2007
    ..we discovered a phage-display selected cyclic peptide motif that was shown to bind selectively to NeutrAvidin and avidin but not to the structurally similar streptavidin...

Research Grants62

  1. Antibody-mediated Gene Therapy for the Treatment of Cancer
    TRACY RUTH DANIELS-WELLS; Fiscal Year: 2013
    ..Therefore, the first level of my targeted strategy will occur through the use of a mouse/human chimeric antibody-avidin fusion protein specific for the transferrin receptor (TfR)...
  2. High-throughput multiplex microsphere screening for toxin protease inhibitors
    Steven W Graves; Fiscal Year: 2012
    ..microsphere assays and two FRET protease assays, which use a simple FRET peptide or a fluorescently tagged avidin and our biotinylated fusion proteins containing full-length protease substrate and a terminal GFP tag...
  3. Carcinogenic Metabolites Formed from Antiestrogen
    Judy L Bolton; Fiscal Year: 2013
    ..The targeted proteins will be isolated using avidin affinity chromatography, separated by 2D electrophoresis, digested, and analyzed by MALDI-TOF and LC-MS-MS...
  4. Role of redox state in ovarian cancer response to cisplatin
    Larry W Daniel; Fiscal Year: 2013
    ..This approach involves labeling modified proteins with biotin derivatives of dimedone, followed by avidin pull-down and analysis by western blotting and mass spectrometry...
  5. A Multi-Purpose Nanovehicle for Targeted Boron Neutron Capture Cancer Therapy &I
    Andrew Webb; Fiscal Year: 2012
    ..We have taken advantage of the strong non-covalent interaction between biotin and avidin to design the platform and its applications...
  6. Artificial Metalloproteins: Confining Metal Complexes within Protein Hosts. (Orig
    Andrew S Borovik; Fiscal Year: 2013
    ..Protein hosts streptavidin and avidin serve as new binding sites for synthetic metal complexes: these proteins do not normally bind metal ions but have ..
  7. Engineering Multi-Tissue Interfaces
    Paul H Krebsbach; Fiscal Year: 2012
    ..be developed in this specific aim to gain maximal control of viral release through a dynamic equilibrium of biotin/avidin and biomaterial interactions. 2...
  8. Molecular UBM and MRI of Vascular Development
    Daniel H Turnbull; Fiscal Year: 2013
    ..recently demonstrated that biotinylation of cell surfaces can be achieved, allowing cell-targeted imaging with avidin-conjugated contrast agents, which are now available for both ultrasound and MRI...
  9. Targeted delivery of PCBP2 siRNA for treating alcoholic liver fibrosis
    Kun Cheng; Fiscal Year: 2013
    ..The overall objectives in the application are two-fold: 1) to develop the avidin-based nanocomplex to overcome the two potential obstacles (poor stability and lack of target-ability to HSCs) of ..
  10. Tailored antigen specificity for personalized adoptive T cell therapy of cancer
    Daniel J Powell; Fiscal Year: 2012
    ..The BBIR system capitalizes on the extremely tight and specific affinity between avidin (or anti-biotin antibody) and biotin in the development of a flexible CIR platform comprised of a biotin- binding ..
  11. BIOSPECIFIC POLYMER ENZYME CONJUGATES FOR DRUG DELIVERY
    GLEN KWON; Fiscal Year: 2004
    ..fractionating in terms of number of attached biotinylated PEG on CPA using an immobilized monomeric avidin. A biotin moiety may tether an antibody in conjunction with biotin and streptavidin...
  12. FLUORESCENT GENE MAPPING AT INTERPHASE & METAPHASE
    Jeanne Lawrence; Fiscal Year: 1993
    ..of single-copy genes or transcripts within interphase nuclei utilizing biotinated probes detected by fluorescein-avidin. This method provides significant advantages in resolution, speed and convenience for metaphase chromosome mapping ..
  13. VASOTOCIN RECEPTORS
    PATRICK EGGENA; Fiscal Year: 1990
    ..sucrose- density gradient centrifugation, affinity chromatography with a biotinyl derivative of AVT linked to an avidin column, and photoaffinity labeling of receptors with radio-active AVT analogues followed by SDS-PAGE...
  14. LATENCY AND LEUKOMOGENICITY OF BOVINE LEUKEMIA VIRUS
    Gary Cockerell; Fiscal Year: 1991
    ..An avidin-biotin complex immunocytochemical technique will be used to determine the number of BLV gp51 antigen- positive ..
  15. REPAIR OF HAIR BUNDLES IN SEA ANEMONES
    GLEN WATSON; Fiscal Year: 1999
    ..Provided such proteins retain biological activity, they will be used in combination with avidin-fluorochromes or with avidin-colloidal gold to localize binding sites for repair proteins on the anemone at the ..
  16. COLLAGENS OF HYALINE CARTILAGE
    Richard Mayne; Fiscal Year: 1992
    ..For high resolution EM immunolocalization, avidin-colloidal gold will be used with biotinylated antibodies...
  17. BIOTIN REQUIREMENTS OF INFANTS
    Donald Mock; Fiscal Year: 1992
    ..A more sensitive, chemically specific biotin assay based on 125I-avidin and HPLC has been developed that would allow measurement of plasma biotin and urinary excretion of biotin and ..
  18. MECHANISMS OF MICROCYSTIN-LR HEPATOTOXICITY
    Val Beasley; Fiscal Year: 1993
    ..e., initially the plasma membrane). Staphylococcus aureus protein A avidin biotin complex (or Streptococcus protein G) will be bound to colloidal gold to detect the antibody binding sites ..
  19. SERUM PROTEIN BINDING THE HN AUTOANTIGEN
    JOHN KANALAS; Fiscal Year: 1993
    ..proteins will be analyzed for binding both in vivo and in vitro by iodination of the proteins or by detection with avidin- biotin or colloidal gold antibodies for immunofluorescence and electron microscopy...
  20. ASSAY FOR PNEUMOCYSTOSIS IN IMMUNODEFICIENT HOSTS
    DON GRAVES; Fiscal Year: 1993
    ..Dot blot and in situ hybridization and the avidin-biotin complex immunoperoxidase techniques will be used...
  21. AVIDIN-BIOTIN TECHNOLOGY FOR HORMONE RECEPTOR ISOLATION
    Klaus Hofmann; Fiscal Year: 1990
    ..If successful, we shall attempt to isolate the solubilized receptor by avidin-biotin technology...
  22. BIOLUMINESCENT PROTEINS AS LABELS IN DIAGNOSTIC ASSAYS
    MILTON CORMIER; Fiscal Year: 1992
    ..aequorin and luciferase will be prepared and tested for stability, sensitivity to detection, and affinity to avidin. Aim #2 Apoaequorin, aequorin and luciferase will be covalently coupled to streptavidin and/or avidin to prepared ..
  23. Anti-TfR IgG3-Av: A New Drug Against Multiple Myeloma
    MANUEL PENICHET; Fiscal Year: 2009
    We have developed an antibody fusion protein composed of avidin fused to a human IgG3 specific for the transferrin receptor (TfR)...
  24. IMPROVED SECOND ANTIBODY RADIOIMMUNOTHERAPY OF CANCER
    ROBERT SHARKEY; Fiscal Year: 2000
    ..Preclinical studies will continue to evaluate the avidin (or streptavidin) biotin pretargeting methodology (A-B method)...
  25. Proteomic Study of Megakaryocyte Transcriptional Control
    Alan Cantor; Fiscal Year: 2007
    ..A novel method employing metabolic biotin tagging followed by avidin affinity chromatography will be used to isolate GATA-1 containing complexes...
  26. PHOSPHOINOSITIDES IN NORMAL AND ABNORMAL HUMAN RED CELLS
    George Dale; Fiscal Year: 1992
    ..The reinfusion of the derivatized cells and their eventual recovery, after in vivo aging, by attachment to an avidin support...
  27. CHEMILUMINESCENT MULTIPLEX GENOME MAPPING
    John Fox; Fiscal Year: 1992
    ..1 hour) of biotinylated end-specific DNA probes to their targets on nylon filters is followed by binding of avidin-alkaline phosphatase conjugates. A chemiluminescent substrate for the enzyme is supplied and light is produced...
  28. HORMONE STORAGE AND SECRETION BY GONADOTROPES
    Gwen Childs; Fiscal Year: 1993
    ..GnRH receptors,beta subunit antigens or mRNAs will be detected by labeled avidin protocols...
  29. Single Molecule Studies of Enzyme Catalysis
    Gordon Hammes; Fiscal Year: 2005
    ..be labeled with biotin, usually as a tag on the amino terminus of the enzyme, and attached to a slide to which avidin is tightly bound at a dilute concentration...
  30. CHARACTERIZATION OF RNA POL II TRANSCRIPTION PROTEINS
    Gyanendra Kumar; Fiscal Year: 1991
    ..individual protein binding domains) to cellulose, either by direct chemical linkage or via a cellulose-biotin-avidin-biotinylated DNA complex...
  31. MONOCLONAL ANTIBODIES FOR IN VIVO LABELED BLOOD PRODUCTS
    Mary Rusckowski; Fiscal Year: 1992
    ..antibody as a vehicle by which to radiolabel T-helper cells in vitro and in vivo by using a novel technique of an avidin linked monoclonal antibody and a subsequent 111-In-biotin as the radiotracer...
  32. AB FUSION PROTEINS AS SYSTEMIC BRAIN DELIVERY VEHICLES
    Sherie Morrison; Fiscal Year: 2004
    ..Proteins that they will produce include an anti-TfR-avidin fusion protein which will be used to deliver a biotinylated anti-tumor antibody, an anti-tumor- Tf fusion protein, ..
  33. LONG-TERM DEPRESSION IN HIPPOCAMPAL INTERNEURONS
    JULIE KAUER; Fiscal Year: 1999
    ..After recording, cells will be labelled with biocytin and reacted with avidin-HRP and anti-glutamate decarboxylase antibody to confirm their morphology, and GABAergic identity.
  34. PHASE I STUDY OF ANTICD20/STREPTAVIDIN
    Hazel Breitz; Fiscal Year: 2001
    ..coli. This fusion protein will be first evaluated in patients as a carrier for avidin binding reactivity to sites of known lymphoma...
  35. Innovative molecular cytogenetic techniques for early leukemia/lymphoma detection
    JOE LUCAS; Fiscal Year: 2005
    ..biotin-labeled repetitive-sequence DNA complex with phenol and chloroform and a second subtraction using avidin labeled magnetic beads. The unique sequences are then recovered using our dual unique sequence primers...
  36. Antisense imaging of brain gene expression in vivo
    William Pardridge; Fiscal Year: 2003
    ..The conjugation of the antisense radiopharmaceuticals to the drug targeting vector is facilitated with the use of avidin- biotin technology...
  37. Targeted Delivery of siRNA for Intravenous RNAi
    RUBEN BOADO; Fiscal Year: 2009
    ..In the present work, the siRNA is mono-biotinylated in parallel with the production of a fusion protein of avidin and a receptor specific monoclonal antibody (MAb)...
  38. FUNCTION OF KINETOPLAST IN THE HEMOFLAGELLATES
    Larry Simpson; Fiscal Year: 1999
    ..Fingerprints of specific RNase digests will be used, as well as biotin-avidin selection of labeled fragments and direct cloning and sequencing...
  39. CHARACTERIZATION OF THE E COLI ENTEROTOXIN ST I RECEPTOR
    Jean Gariepy; Fiscal Year: 1991
    ..of ST I to an agarose matrix or by derivatizing analogues of ST I with a biotin group, in an effort to use the avidin-biotin interaction to fish out the St I-receptor complex...
  40. RRNA/TRNA INTERACTIONS IN PEPTIDYL TRANSFERASE CENTER
    Alexander Mankin; Fiscal Year: 1999
    ..attachment of biotin to the anticodon end of peptidyl tRNAs and immobilizing the resulting tRNA derivatives on an avidin column...
  41. A Novel Antibody Therapeutic for Multiple Myeloma
    Koteswara Chintalacharuvu; Fiscal Year: 2004
    ..Based on the observation that anti-TfR fused to avidin that exists in a dimeric form with four antigen binding sites shows anti-proliferative and pro-apoptotic ..
  42. AMPLIFIED FLUORESCENT REPORTERS FOR AVIDIN-BIOTIN TESTS
    ALEXANDER WEIS; Fiscal Year: 2000
    The long-term objectives of the proposed research include preparation of avidin, streptavidin, and biotin derivatives labeled with amplified fluorescent tags for use in a variety of avidin-biotin technology applications, including ..
  43. Synthetic vectors for pH-targeted gene delivery
    David Oupicky; Fiscal Year: 2006
    ..polyplexes will be investigated by analyzing pH-dependent binding of biotin-containing polyplexes to immobilized avidin and by measuring the levels of pH-dependent cellular uptake of non-specifically-binding and integrin- and ..
  44. PEPTIDE FOR GENTLE PURIFICATION OF BIOTINYLATED PROTEIN
    Ronald Gill; Fiscal Year: 2001
    ..The avidin-biotin complex has the highest affinity interaction known in nature, and this characteristic has made the avidin-..
  45. BIFUNCTIONAL CHELATES IN CANCER IMAGING AND THERAPY
    David Goodwin; Fiscal Year: 1992
    ..tumor mouse distribution of new bifunctional antibodies with dual specificity for tumor and chelate, use of an avidin- biotin system in a pretargeted immunoscintigraphy method with 1-2 hr images using Tc-99m and Ga-68 and therapy ..
  46. PHOTOCLEAVABLE REAGENTS FOR ISOLATION OF STEM CELLS
    Jerzy Olejnik; Fiscal Year: 2001
    ..While progress has been made in the isolation of stem cells through biotin-avidin based immunoadsorption, improvements are needed in order to increase yields, purity and rapidity of processing of ..
  47. CHARACTERIZATION OF CEREBRAL AMYLOID ANGIOPATHIC PEPTIDE
    Harry Vinters; Fiscal Year: 1992
    ..isolated proteins described above, done at light microscopic and ultrastructural levels using (respectively) an avidin-biotin peroxidase technique and protein A-gold methods. Immunohistochemistry of other AD hallmarks (e.g...
  48. HORMONE REGULATION & STRUCTURE OF ACETYL-COA CARBOXYLASE
    SALIH WAKIL; Fiscal Year: 1990
    ..The orientation of the subunits in the dimer form will be determined by electron microscopy of negatively stained avidin-carboxylase complexes...
  49. HISTONE DEPOSITION IN VIRALLY-INFECTED AND TRANSFORMED
    Vaughn Jackson; Fiscal Year: 1990
    ..newly developed procedures of nick translation of nuclei with biotinylated nucleotide to isolate active genes on avidin-agarose...
  50. FREEZE-TRANSFER METHOD FOR IN SITU DETECTION OF RNA
    CHARLES MCGRATH; Fiscal Year: 1990
    ..methods give the required sensitivity for these low abundance mRNAs, and these methods are inferior to biotin/avidin-based methods for time, safety and other reasons...
  51. Imaging and Treatment of Gliomas Using Nanoparticles
    G Reddy; Fiscal Year: 2003
    ..We propose to demonstrate that attachment of RGD containing peptides or Avidin to Gd-containing nanoparticles results in enhanced binding and internalization...
  52. MEMBRANE PROTEINS IN TUMORS
    MASARU IMADA; Fiscal Year: 1980
    ..Biotin molecules are then complexed with avidin-horseradish peroxidase conjugate...
  53. Targeting the Blood-Brain Barrier in Neuroinflammation
    Ulrich Bickel; Fiscal Year: 2006
    ..will be bound in a complex by the cationic polymer polyethylenimine, and then coupled to the 8D3 antibody via an avidin-biotin bridge...
  54. AFFINITY PURIFICATION OF LARGE FRAGMENTS OF HUMAN DNA
    David Ward; Fiscal Year: 1992
    ..Affinity resins, such as avidin-agarose, suitable for the selective capture and subsequent recovery of specific biotinylated hybrid molecules, ..
  55. MaxiK channel biology
    LIGIA TORO DE STEFANI; Fiscal Year: 2009
    ..Slo can be myristoylated. We will use a multidisciplinary approach, in particular, avidin-Slo constructs for visualization of single-molecule movements with quantum dots and high resolution confocal ..
  56. Synthesis and Characterization of Leukopolymersomes
    JAMES SILAS; Fiscal Year: 2004
    ..with either a primary amine terminated ligand, or through a hierarchical strategy that takes advantage of biotin-avidin binding and the availability of biotinylated ligands...
  57. LUMINESCENCE DETECTION REAGENTS FOR PROTEINS
    NANCY STULTS; Fiscal Year: 1990
    ..of both RecAequorin and RecLuciferase as direct substitutes for the commonly used enzyme conjugates such as avidin/alkaline phosphatase...
  58. CHROMOSOME DOMAINS IN INTERPHASE CELLS
    David Ward; Fiscal Year: 1993
    ..using subtractive hybridization with appropriate biotinylated driver probes and affinity fractionation over avidin- metal chelate resins...
  59. PROTEASES AND ANTIPROTEASES IN LUNG: EFFECTS OF SMOKING
    Sanford Simon; Fiscal Year: 1991
    ..reagent, followed by established secondary-antibody immunohistochemical methods (peroxidase-antiperoxidase, avidin-biotin)...
  60. BIOCHEMICAL STUDY OF EMBRYONICALLY REGULATED HISTONE
    JOHN LANGMORE; Fiscal Year: 1993
    ..Immobilization of the hybrids is achieved on an Avidin D affinity matrix...
  61. Optimizing Foamy Virus Vectors for Gene Therapy & Vaccine Applications
    WILLAM HARDY; Fiscal Year: 2006
    ..Insertion of the IgG-binding domain of protein G as well as avidin into the Sindbis virus envelope E2 region will also be carried out to extend the utility of this vector...
  62. Labeless Analysis of Microarrays by Force Microscopy
    David Allison; Fiscal Year: 2005
    ..These optimizations will be performed using a standard system consisting of a biotin tethered microcantilever and avidin tethered to a surface...