acetylglucosamine

Summary

Summary: The N-acetyl derivative of glucosamine.

Top Publications

  1. pmc Characterization of O-acetylation of N-acetylglucosamine: a novel structural variation of bacterial peptidoglycan
    Elvis Bernard
    INRA, UMR1319 MICALIS, F 78350 Jouy en Josas, France
    J Biol Chem 286:23950-8. 2011
  2. ncbi The absence of fucose but not the presence of galactose or bisecting N-acetylglucosamine of human IgG1 complex-type oligosaccharides shows the critical role of enhancing antibody-dependent cellular cytotoxicity
    Toyohide Shinkawa
    Tokyo Research Laboratories, Kyowa Hakko Kogyo Co, Ltd, 3 6 6 Asahi machi, Machida shi, Tokyo 194 8533, Japan
    J Biol Chem 278:3466-73. 2003
  3. pmc Hyperglycemia inhibits endothelial nitric oxide synthase activity by posttranslational modification at the Akt site
    X L Du
    Diabetes Research Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Clin Invest 108:1341-8. 2001
  4. pmc Colistin-resistant, lipopolysaccharide-deficient Acinetobacter baumannii responds to lipopolysaccharide loss through increased expression of genes involved in the synthesis and transport of lipoproteins, phospholipids, and poly-β-1,6-N-acetylglucosamine
    Rebekah Henry
    Department of Microbiology, Monash University, Clayton, Australia
    Antimicrob Agents Chemother 56:59-69. 2012
  5. pmc O-GlcNAc signalling: implications for cancer cell biology
    Chad Slawson
    The Department of Biochemistry and Molecular Biology, Kansas University School of Medicine, 3, 901 Rainbow Boulevard, Kansas City, Kansas 66160, USA
    Nat Rev Cancer 11:678-84. 2011
  6. pmc Differential roles of poly-N-acetylglucosamine surface polysaccharide and extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis biofilms
    Era A Izano
    Medical Science Building, Room C 636, 185 S Orange Ave, Newark, NJ 07103, USA
    Appl Environ Microbiol 74:470-6. 2008
  7. pmc Beta-N-acetylglucosamine (O-GlcNAc) is part of the histone code
    Kaoru Sakabe
    Department of Biological Chemistry, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:19915-20. 2010
  8. pmc Modification of histones by sugar β-N-acetylglucosamine (GlcNAc) occurs on multiple residues, including histone H3 serine 10, and is cell cycle-regulated
    Suisheng Zhang
    Genome Stability Laboratory, Center for Chromosome Biology, School of Natural Sciences, National University of Ireland Galway, University Road, Galway, Ireland
    J Biol Chem 286:37483-95. 2011
  9. pmc O-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation
    Takahiro Isono
    Central Research Laboratory, Shiga University of Medical Science, Otsu, Shiga, Japan
    PLoS ONE 6:e18959. 2011
  10. pmc Critical role of O-Linked β-N-acetylglucosamine transferase in prostate cancer invasion, angiogenesis, and metastasis
    Thomas P Lynch
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102, USA
    J Biol Chem 287:11070-81. 2012

Detail Information

Publications289 found, 100 shown here

  1. pmc Characterization of O-acetylation of N-acetylglucosamine: a novel structural variation of bacterial peptidoglycan
    Elvis Bernard
    INRA, UMR1319 MICALIS, F 78350 Jouy en Josas, France
    J Biol Chem 286:23950-8. 2011
    ..We report here the presence of O-acetylation on N-acetylglucosamine (GlcNAc) in Lactobacillus plantarum PG. This modification of glycan strands was never described in bacteria...
  2. ncbi The absence of fucose but not the presence of galactose or bisecting N-acetylglucosamine of human IgG1 complex-type oligosaccharides shows the critical role of enhancing antibody-dependent cellular cytotoxicity
    Toyohide Shinkawa
    Tokyo Research Laboratories, Kyowa Hakko Kogyo Co, Ltd, 3 6 6 Asahi machi, Machida shi, Tokyo 194 8533, Japan
    J Biol Chem 278:3466-73. 2003
    ....
  3. pmc Hyperglycemia inhibits endothelial nitric oxide synthase activity by posttranslational modification at the Akt site
    X L Du
    Diabetes Research Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Clin Invest 108:1341-8. 2001
    ..Since hyperglycemia-induced mitochondrial superoxide overproduction increases O-linked N-acetylglucosamine modification and decreases O-linked phosphorylation of the transcription factor Sp1, the effect of ..
  4. pmc Colistin-resistant, lipopolysaccharide-deficient Acinetobacter baumannii responds to lipopolysaccharide loss through increased expression of genes involved in the synthesis and transport of lipoproteins, phospholipids, and poly-β-1,6-N-acetylglucosamine
    Rebekah Henry
    Department of Microbiology, Monash University, Clayton, Australia
    Antimicrob Agents Chemother 56:59-69. 2012
    ..In addition, genes involved in the synthesis and transport of poly-β-1,6-N-acetylglucosamine (PNAG) also were upregulated, and a corresponding increase in PNAG production was observed...
  5. pmc O-GlcNAc signalling: implications for cancer cell biology
    Chad Slawson
    The Department of Biochemistry and Molecular Biology, Kansas University School of Medicine, 3, 901 Rainbow Boulevard, Kansas City, Kansas 66160, USA
    Nat Rev Cancer 11:678-84. 2011
    O-GlcNAcylation is the covalent attachment of β-D-N-acetylglucosamine (GlcNAc) sugars to serine or threonine residues of nuclear and cytoplasmic proteins, and it is involved in extensive crosstalk with other post-translational ..
  6. pmc Differential roles of poly-N-acetylglucosamine surface polysaccharide and extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis biofilms
    Era A Izano
    Medical Science Building, Room C 636, 185 S Orange Ave, Newark, NJ 07103, USA
    Appl Environ Microbiol 74:470-6. 2008
    ..In this study, we investigated the functions of two staphylococcal biofilm matrix polymers: poly-N-acetylglucosamine surface polysaccharide (PNAG) and extracellular DNA (ecDNA)...
  7. pmc Beta-N-acetylglucosamine (O-GlcNAc) is part of the histone code
    Kaoru Sakabe
    Department of Biological Chemistry, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:19915-20. 2010
    Dynamic posttranslational modification of serine and threonine residues of nucleocytoplasmic proteins by β-N-acetylglucosamine (O-GlcNAc) is a regulator of cellular processes such as transcription, signaling, and protein-protein ..
  8. pmc Modification of histones by sugar β-N-acetylglucosamine (GlcNAc) occurs on multiple residues, including histone H3 serine 10, and is cell cycle-regulated
    Suisheng Zhang
    Genome Stability Laboratory, Center for Chromosome Biology, School of Natural Sciences, National University of Ireland Galway, University Road, Galway, Ireland
    J Biol Chem 286:37483-95. 2011
    The monosaccharide, β-N-acetylglucosamine (GlcNAc), can be added to the hydroxyl group of either serines or threonines to generate an O-linked β-N-acetylglucosamine (O-GlcNAc) residue (Love, D. C., and Hanover, J. A. (2005) Sci...
  9. pmc O-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation
    Takahiro Isono
    Central Research Laboratory, Shiga University of Medical Science, Otsu, Shiga, Japan
    PLoS ONE 6:e18959. 2011
    Modification of serine and threonine residues in proteins by O-linked β-N-acetylglucosamine (O-GlcNAc) glycosylation is a feature of many cellular responses to the nutritional state and to stress...
  10. pmc Critical role of O-Linked β-N-acetylglucosamine transferase in prostate cancer invasion, angiogenesis, and metastasis
    Thomas P Lynch
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102, USA
    J Biol Chem 287:11070-81. 2012
    ..the hexosamine biosynthetic pathway leads to increases in the post-translational addition of O-linked β-N-acetylglucosamine (O-GlcNAc) to various nuclear and cytosolic proteins...
  11. ncbi GlcNAcylation of histone H2B facilitates its monoubiquitination
    Ryoji Fujiki
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Nature 480:557-60. 2011
    ..In metazoans, reversible protein modification by O-linked N-acetylglucosamine (GlcNAc) is catalysed by two enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA)...
  12. pmc O-GlcNAc signaling: a metabolic link between diabetes and cancer?
    C Slawson
    The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725N Wolfe St Baltimore, MD 21205, USA
    Trends Biochem Sci 35:547-55. 2010
    O-linked β-N-acetylglucosamine (O-GlcNAc) is a sugar attachment to serine or threonine hydroxyl moieties on nuclear and cytoplasmic proteins...
  13. pmc The hexosamine biosynthetic pathway couples growth factor-induced glutamine uptake to glucose metabolism
    Kathryn E Wellen
    Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Genes Dev 24:2784-99. 2010
    ..Treatment of glucose-starved cells with N-acetylglucosamine (GlcNAc) to maintain hexosamine biosynthesis restored mitochondrial metabolism and cell growth by promoting ..
  14. pmc Cross talk between O-GlcNAcylation and phosphorylation: roles in signaling, transcription, and chronic disease
    Gerald W Hart
    Departments of Biological Chemistry and Pediatrics, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205
    Annu Rev Biochem 80:825-58. 2011
    O-GlcNAcylation is the addition of β-D-N-acetylglucosamine to serine or threonine residues of nuclear and cytoplasmic proteins...
  15. ncbi Essential role of the glycosyltransferase sxc/Ogt in polycomb repression
    Maria Cristina Gambetta
    Gene Expression Programme, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Science 325:93-6. 2009
    ..super sex combs (sxc) encodes Ogt, the highly conserved glycosyltransferase that catalyzes the addition of N-acetylglucosamine (GlcNAc) to proteins in animals and plants...
  16. pmc O-linked beta-N-acetylglucosamine (O-GlcNAc): Extensive crosstalk with phosphorylation to regulate signaling and transcription in response to nutrients and stress
    Chutikarn Butkinaree
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Biochim Biophys Acta 1800:96-106. 2010
    Since its discovery in the early 1980s, O-linked-beta-N-acetylglucosamine (O-GlcNAc), a single sugar modification on the hydroxyl group of serine or threonine residues, has changed our views of protein glycosylation...
  17. pmc N-acetylglucosamine induces white-to-opaque switching and mating in Candida tropicalis, providing new insights into adaptation and fungal sexual evolution
    Jing Xie
    State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    Eukaryot Cell 11:773-82. 2012
    ..Here we show that N-acetylglucosamine (GlcNAc), an inducer of white-to-opaque switching in C...
  18. pmc Immunochemical properties of the staphylococcal poly-N-acetylglucosamine surface polysaccharide
    Tomas Maira-Litran
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts, USA
    Infect Immun 70:4433-40. 2002
    ..intercellular adhesin (PIA), which is composed of a approximately 28 kDa soluble linear beta(1-6)-linked N-acetylglucosamine. We developed a new method to purify PS/A from S. aureus MN8m, a strain hyperproducing PS/A...
  19. ncbi Impact of Type 2 diabetes and aging on cardiomyocyte function and O-linked N-acetylglucosamine levels in the heart
    Norbert Fülöp
    Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Am J Physiol Cell Physiol 292:C1370-8. 2007
    Increased levels of O-linked attachment of N-acetylglucosamine (O-GlcNAc) on nucleocytoplasmic proteins are implicated in the development of diabetic cardiomyopathy and are regulated by O-GlcNAc transferase (OGT) expression and its ..
  20. ncbi The hexosamine signaling pathway: deciphering the "O-GlcNAc code"
    Dona C Love
    Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892, USA
    Sci STKE 2005:re13. 2005
    A dynamic cycle of addition and removal of O-linked N-acetylglucosamine (O-GlcNAc) at serine and threonine residues is emerging as a key regulator of nuclear and cytoplasmic protein activity...
  21. pmc O-GlcNAcylation regulates phosphorylation of tau: a mechanism involved in Alzheimer's disease
    Fei Liu
    Department of Neurochemistry, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA
    Proc Natl Acad Sci U S A 101:10804-9. 2004
    ..tau was modified by O-GlcNAcylation, a type of protein O-glycosylation by which the monosaccharide beta-N-acetylglucosamine (GlcNAc) attaches to serine/threonine residues via an O-linked glycosidic bond...
  22. ncbi Nutrient sensor O-GlcNAc transferase regulates breast cancer tumorigenesis through targeting of the oncogenic transcription factor FoxM1
    S A Caldwell
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA, USA
    Oncogene 29:2831-42. 2010
    ..a cell's glucose enters the hexosamine biosynthetic pathway (HBP), which regulates levels of O-linked beta-N-acetylglucosamine (O-GlcNAc), a carbohydrate posttranslational modification of diverse nuclear and cytosolic proteins...
  23. ncbi Phosphoinositide signalling links O-GlcNAc transferase to insulin resistance
    Xiaoyong Yang
    Howard Hughes Medical Institute and Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nature 451:964-9. 2008
    ..pathway leads to the post-translational modification of cytoplasmic and nuclear proteins by O-linked beta-N-acetylglucosamine (O-GlcNAc)...
  24. pmc Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse
    Jonathan C Trinidad
    Department of Pharmaceutical Chemistry, UCSF, San Francisco, California 94158, USA
    Mol Cell Proteomics 11:215-29. 2012
    O-linked N-acetylglucosamine (O-GlcNAc) is a dynamic, reversible monosaccharide modifier of serine and threonine residues on intracellular protein domains. Crosstalk between O-GlcNAcylation and phosphorylation has been hypothesized...
  25. pmc N-acetylglucosamine-6-phosphate deacetylase (NagA) of Listeria monocytogenes EGD, an essential enzyme for the metabolism and recycling of amino sugars
    Magdalena Popowska
    Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Poland
    Arch Microbiol 194:255-68. 2012
    ..monocytogenes is required for cell wall peptidoglycan and teichoic acid biosynthesis and is also essential for bacterial cell growth, cell division, and sensitivity to cell wall hydrolases and peptide antibiotics...
  26. ncbi O-GlcNAc modification, insulin signaling and diabetic complications
    T Issad
    CNRS UMR 8104, Universite Paris Descartes, Institut Cochin, 22 rue Mechain, Paris, France
    Diabetes Metab 36:423-35. 2010
    O-GlcNAc glycosylation (O-GlcNAcylation) corresponds to the addition of N-acetylglucosamine on serine and threonine residues of cytosolic and nuclear proteins...
  27. pmc Tandem mass spectrometry identifies many mouse brain O-GlcNAcylated proteins including EGF domain-specific O-GlcNAc transferase targets
    Joshua F Alfaro
    Pacific Northwest National Laboratory, Richland, WA 99352, USA
    Proc Natl Acad Sci U S A 109:7280-5. 2012
    O-linked N-acetylglucosamine (O-GlcNAc) is a reversible posttranslational modification of Ser and Thr residues on cytosolic and nuclear proteins of higher eukaryotes catalyzed by O-GlcNAc transferase (OGT)...
  28. pmc Modulation of dynamin-related protein 1 (DRP1) function by increased O-linked-β-N-acetylglucosamine modification (O-GlcNAc) in cardiac myocytes
    Thomas Gawlowski
    Department of Medicine, University of California, San Diego, La Jolla, CA 92039, USA
    J Biol Chem 287:30024-34. 2012
    ..In conclusion, this report shows, for the first time, that O-GlcNAcylation modulates DRP1 functionality in cardiac muscle cells...
  29. pmc β-N-Acetylglucosamine (O-GlcNAc) is a novel regulator of mitosis-specific phosphorylations on histone H3
    Jerry J Fong
    Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas 77030 3498, USA
    J Biol Chem 287:12195-203. 2012
    O-Linked β-N-acetylglucosamine, or O-GlcNAc, is a dynamic post-translational modification that cycles on and off serine and threonine residues of nucleocytoplasmic proteins...
  30. pmc Metabolic cross-talk allows labeling of O-linked beta-N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway
    Michael Boyce
    Departments of Chemistry and Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 108:3141-6. 2011
    Hundreds of mammalian nuclear and cytoplasmic proteins are reversibly glycosylated by O-linked β-N-acetylglucosamine (O-GlcNAc) to regulate their function, localization, and stability...
  31. ncbi Dysregulation of the nutrient/stress sensor O-GlcNAcylation is involved in the etiology of cardiovascular disorders, type-2 diabetes and Alzheimer's disease
    Tony Lefebvre
    CNRS UMR 8576, Unit of Structural and Functional Glycobiology, IFR 147, University of Lille 1, Villeneuve d Ascq, France
    Biochim Biophys Acta 1800:67-79. 2010
    ..Lastly, the finding that AD may correspond to a type-3 diabetes offers new perspectives into the knowledge of the neuropathology and into the search for new therapeutic avenues...
  32. pmc O-GlcNAc regulates FoxO activation in response to glucose
    Michael P Housley
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 283:16283-92. 2008
    ..are well established, but FoxOs have not been studied in the context of the glucose-derived O-linked beta-N-acetylglucosamine (O-GlcNAc) modification. Here we show that O-GlcNAc on hepatic FoxO1 is increased in diabetes...
  33. pmc Cross-talk between GlcNAcylation and phosphorylation: roles in insulin resistance and glucose toxicity
    Ronald J Copeland
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205 2185, USA
    Am J Physiol Endocrinol Metab 295:E17-28. 2008
    O-linked beta-N-acetylglucosamine (O-GlcNAc) is a dynamic posttranslational modification that, analogous to phosphorylation, cycles on and off serine and/or threonine hydroxyl groups...
  34. ncbi Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteins
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205 2185, USA
    Nature 446:1017-22. 2007
    All animals and plants dynamically attach and remove O-linked beta-N-acetylglucosamine (O-GlcNAc) at serine and threonine residues on myriad nuclear and cytoplasmic proteins...
  35. pmc The hexosamine signaling pathway: O-GlcNAc cycling in feast or famine
    John A Hanover
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1800:80-95. 2010
    ..It will emphasize the impact O-GlcNAcylation has upon signaling pathways that may be become deregulated in diseases of the immune system, diabetes mellitus, cancer, cardiovascular disease, and neurodegenerative diseases...
  36. ncbi Site-specific interplay between O-GlcNAcylation and phosphorylation in cellular regulation
    Ping Hu
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, MD 21205 2185, USA
    FEBS Lett 584:2526-38. 2010
    Ser(Thr)-O-linked beta-N-acetylglucosamine (O-GlcNAc) is a ubiquitous modification of nucleocytoplasmic proteins...
  37. ncbi Characterization of a mouse monoclonal antibody specific for O-linked N-acetylglucosamine
    F I Comer
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205-2185, USA
    Anal Biochem 293:169-77. 2001
    beta-O-linked N-acetylglucosamine (O-GlcNAc) is an abundant posttranslational modification of resident nuclear and cytoplasmic proteins in eukaryotes...
  38. pmc O-GlcNAcylation of kinases
    Wagner B Dias
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA
    Biochem Biophys Res Commun 422:224-8. 2012
    ..Our results suggest that O-GlcNAcylation may directly regulate a substantial number of kinases and illustrates the increasingly complex relationship between O-GlcNAcylation and phosphorylation in cellular signaling...
  39. pmc N-acetylglucosamine induces white to opaque switching, a mating prerequisite in Candida albicans
    Guanghua Huang
    Department of Biology, The University of Iowa, Iowa City, Iowa, United States of America
    PLoS Pathog 6:e1000806. 2010
    ..Here, we demonstrate that a second signal, N-acetylglucosamine (GlcNAc), a monosaccharide produced primarily by gastrointestinal tract bacteria, also serves as a potent ..
  40. pmc N-acetylglucosamine (GlcNAc) induction of hyphal morphogenesis and transcriptional responses in Candida albicans are not dependent on its metabolism
    Shamoon Naseem
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794 5222, USA
    J Biol Chem 286:28671-80. 2011
    N-acetylglucosamine (GlcNAc) stimulates important signaling pathways in a wide range of organisms...
  41. pmc The bisecting GlcNAc in cell growth control and tumor progression
    Hazuki E Miwa
    Department of Cell Biology, Albert Einstein College of Medicine, New York, NY 10461, USA
    Glycoconj J 29:609-18. 2012
    ..This review will describe evidence that the bisecting GlcNAc of N-glycans regulates cellular signaling and tumor progression, apparently through modulating N-glycan/galectin interactions...
  42. ncbi Distribution of chitinases in the entomopathogen Metarhizium anisopliae and effect of N-acetylglucosamine in protein secretion
    Cristine Chaves Barreto
    Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Campus do Vale, P O Box 15005, 91501 970 Porto Alegre, RS, Brazil
    Curr Microbiol 48:102-7. 2004
    ..Various sugar combinations were investigated for induction and repression of chitinase. N-acetylglucosamine (GlcNAc) shows a special dual regulation on chitinase production. M...
  43. pmc N-acetylglucosamine: production and applications
    Jeen Kuan Chen
    Department of Environment and Biotechnology, Refining and Manufacturing Research Institute, CPC Corporation, 217 Min Sheng S Rd, Chiayi, Taiwan
    Mar Drugs 8:2493-516. 2010
    N-Acetylglucosamine (GlcNAc) is a monosaccharide that usually polymerizes linearly through (1,4)-β-linkages. GlcNAc is the monomeric unit of the polymer chitin, the second most abundant carbohydrate after cellulose...
  44. pmc Regulon of the N-acetylglucosamine utilization regulator NagR in Bacillus subtilis
    Ralph Bertram
    Lehrbereich Mikrobielle Genetik, Eberhard Karls Universitat Tubingen, Waldhäuser Strasse 70 8, 72076 Tubingen, Germany
    J Bacteriol 193:3525-36. 2011
    N-Acetylglucosamine (GlcNAc) is the most abundant carbon-nitrogen biocompound on earth and has been shown to be an important source of nutrients for both catabolic and anabolic purposes in Bacillus species...
  45. pmc Gene expression of O-GlcNAc cycling enzymes in human breast cancers
    Anna Krzeslak
    Department of Cytobiochemistry, University of Lodz, Łódź, Poland
    Clin Exp Med 12:61-5. 2012
    O-GlcNAcylation is an abundant, dynamic, and inducible posttranslational modification in which single β-N-acetylglucosamine residues are attached by O-glycosidic linkage to serine or treonine residues...
  46. pmc Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis
    Zihao Wang
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Sci Signal 3:ra2. 2010
    Like phosphorylation, the addition of O-linked beta-N-acetylglucosamine (O-GlcNAcylation) is a ubiquitous, reversible process that modifies serine and threonine residues on nuclear and cytoplasmic proteins...
  47. pmc The elastic properties of the caulobacter crescentus adhesive holdfast are dependent on oligomers of N-acetylglucosamine
    Guanglai Li
    Physics Department, Brown University, Providence, RI 02912, USA
    J Bacteriol 187:257-65. 2005
    ..In particular, the contribution of oligomers of N-acetylglucosamine (GlcNAc) to the elasticity of holdfast was examined by lysozyme digestion. C...
  48. pmc Modulators of sensitivity and resistance to inhibition of PI3K identified in a pharmacogenomic screen of the NCI-60 human tumor cell line collection
    Kevin A Kwei
    Genomic Health, Inc, Redwood City, California, United States of America
    PLoS ONE 7:e46518. 2012
    ..These candidate biomarkers will offer useful tools to begin a more thorough understanding of determinants of patient response to PI3K inhibitors and merit exploration in human cancer patients treated with PI3K inhibitors...
  49. ncbi Diverse regulation of protein function by O-GlcNAc: a nuclear and cytoplasmic carbohydrate post-translational modification
    Keith Vosseller
    Johns Hopkins University School of Medicine, Department of Biological Chemistry, Baltimore, MD 21218, USA
    Curr Opin Chem Biol 6:851-7. 2002
    N-Acetylglucosamine O-linked to serines and threonines of cytosolic and nuclear proteins (O-GlcNAc) is an abundant reversible post-translational modification found in all higher eukaryotes...
  50. ncbi Analysis of the human cancer glycome identifies a novel group of tumor-associated N-acetylglucosamine glycan antigens
    Tero Satomaa
    Glykos Finland Ltd, Helsinki, Finland
    Cancer Res 69:5811-9. 2009
    ..In conclusion, the identified terminal GlcNAc glycans should be recognized as a novel class of tumor markers...
  51. pmc dbOGAP - an integrated bioinformatics resource for protein O-GlcNAcylation
    Jinlian Wang
    Department of Oncology, Georgetown University Medical Center, NW, Washington, DC 20007, USA
    BMC Bioinformatics 12:91. 2011
    Protein O-GlcNAcylation (or O-GlcNAc-ylation) is an O-linked glycosylation involving the transfer of β-N-acetylglucosamine to the hydroxyl group of serine or threonine residues of proteins...
  52. pmc Characterization of the opsonic and protective activity against Staphylococcus aureus of fully human monoclonal antibodies specific for the bacterial surface polysaccharide poly-N-acetylglucosamine
    Casie Kelly-Quintos
    Harvard Medical School, 181 Longwood Ave, Boston, MA 02115, USA
    Infect Immun 74:2742-50. 2006
    ....
  53. pmc O-GlcNAc signaling in the cardiovascular system
    Gladys A Ngoh
    Institute of Molecular Cardiology, University of Louisville, 580 South Preston St, 404C, Baxter II 404C, Louisville, KY 40202, USA
    Circ Res 107:171-85. 2010
    ..Known as the beta-O-linkage of N-acetylglucosamine (ie, O-GlcNAc) to cellular proteins, this unique monosaccharide is involved in a diverse array of ..
  54. ncbi L-ficolin is a pattern recognition molecule specific for acetyl groups
    Anders Krarup
    Institute of Medical Microbiology and Immunology, University of Aarhus, 8000 Aarhus, Denmark
    J Biol Chem 279:47513-9. 2004
    ..the selectivity of the ficolins through inhibiting the binding to bacteria or to beads coupled with N-acetylglucosamine. The binding of L-ficolin to Streptococcus pneumoniae 11F and the beads was inhibited by N-acetylated sugars ..
  55. ncbi O-GlcNAc modification of FoxO1 increases its transcriptional activity: a role in the glucotoxicity phenomenon?
    Meishiue Kuo
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Biochimie 90:679-85. 2008
    ....
  56. ncbi A novel human serum lectin with collagen- and fibrinogen-like domains that functions as an opsonin
    M Matsushita
    Department of Biochemistry, Fukushima Medical College, Japan
    J Biol Chem 271:2448-54. 1996
    ..Therefore, P35 represents a new type of GlcNAc-binding lectin with structural and functional similarities to collectins involved in innate immunity...
  57. pmc Modulation of transcription factor function by O-GlcNAc modification
    Sabire Ozcan
    Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA
    Biochim Biophys Acta 1799:353-64. 2010
    O-linked beta-N-acetylglucosamine (O-GlcNAc) modification of nuclear and cytoplasmic proteins is important for many cellular processes, and the number of proteins that contain this modification is steadily increasing...
  58. ncbi Identification of O-linked N-acetylglucosamine proteins in rat skeletal muscle using two-dimensional gel electrophoresis and mass spectrometry
    Caroline Cieniewski-Bernard
    Unite Mixte de Recherche, Centre National de la Recherche Scientifique 8576, Glycobiologie Structurale et Fonctionnelle, IFR118, Villeneuve d Ascc, France
    Mol Cell Proteomics 3:577-85. 2004
    ..as a nutritional sensor, the level of UDP-GlcNAc that serves as a donor for the uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetyl-glucosaminyltransferase being regulated by the cellular fate of glucose...
  59. ncbi LysM, a widely distributed protein motif for binding to (peptido)glycans
    Girbe Buist
    Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    Mol Microbiol 68:838-47. 2008
    ..65 amino acid residues and binds to various types of peptidoglycan and chitin, most likely recognizing the N-acetylglucosamine moiety...
  60. ncbi Increased hexosamine biosynthesis and protein O-GlcNAc levels associated with myocardial protection against calcium paradox and ischemia
    Jia Liu
    Department of Cell Biology and Medicine, University of Alabama at Birmingham, 1530 3rd Avenue South, MCLM 690, Birmingham, AL 35294 0005, USA
    J Mol Cell Cardiol 40:303-12. 2006
    Increased hexosamine biosynthesis pathway (HBP) flux and elevated levels of protein O-linked-N-acetylglucosamine (O-GlcNAc) decrease calcium influx into isolated cardiomyocytes...
  61. ncbi In silico modulation of HMGN-1 binding to histones and gene expression by interplay of phosphorylation and O-GlcNAc modification
    Ishtiaq Ahmad
    Institute of Molecular Sciences and Bioinformatics, Lahore, Pakistan
    Protein Pept Lett 15:193-9. 2008
    ....
  62. pmc Increased enzymatic O-GlcNAcylation of mitochondrial proteins impairs mitochondrial function in cardiac myocytes exposed to high glucose
    Yong Hu
    Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 284:547-55. 2009
    Increased nuclear protein O-linked beta-N-acetylglucosamine glycosylation (O-GlcNAcylation) mediated by high glucose treatment or the hyperglycemia of diabetes mellitus contributes to cardiac myocyte dysfunction...
  63. pmc Dysregulation of insulin signaling, glucose transporters, O-GlcNAcylation, and phosphorylation of tau and neurofilaments in the brain: Implication for Alzheimer's disease
    Yanqiu Deng
    Department of Neurochemistry, New York State Institute for Basic Research in Developmental Disabilities, Staten Island, New York, NY 10314, USA
    Am J Pathol 175:2089-98. 2009
    ....
  64. pmc Regulation of calcium/calmodulin-dependent kinase IV by O-GlcNAc modification
    Wagner B Dias
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 284:21327-37. 2009
    ..This is the first example of an O-GlcNAc/phosphate cycle involving O-GlcNAc transferase/kinase cross-talk...
  65. pmc Inactivation of phosphorylated endothelial nitric oxide synthase (Ser-1177) by O-GlcNAc in diabetes-associated erectile dysfunction
    Biljana Musicki
    Department of Urology, The Johns Hopkins Hospital, 600 North Wolfe Street, Baltimore, MD 21287, USA
    Proc Natl Acad Sci U S A 102:11870-5. 2005
    ..In vivo penile erection paradigm supports the physiologic relevance of O-GlcNAc modification in vascular disorders associated with diabetes...
  66. pmc Hyperglycemia-induced mitochondrial superoxide overproduction activates the hexosamine pathway and induces plasminogen activator inhibitor-1 expression by increasing Sp1 glycosylation
    X L Du
    Albert Einstein College of Medicine, Diabetes Research Center, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 97:12222-6. 2000
    ..Thus, hyperglycemia-induced mitochondrial superoxide overproduction increases hexosamine synthesis and O-glycosylation of Sp1, which activates expression of genes that contribute to the pathogenesis of diabetic complications...
  67. pmc The O-GlcNAc transferase gene resides on the X chromosome and is essential for embryonic stem cell viability and mouse ontogeny
    R Shafi
    The Howard Hughes Medical Institute, Glycobiology Research and Training Center, Department of Cellular and Molecular Medicine, 9500 Gilman Drive 0625, University of California San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 97:5735-9. 2000
    ..Intracellular glycosylation by the addition of N-acetylglucosamine (GlcNAc) to serine and threonine is catalyzed by the O-GlcNAc transferase (OGT)...
  68. pmc O-GlcNAc cycling: implications for neurodegenerative disorders
    Brooke D Lazarus
    Laboratory of Cell Biology and Biochemistry, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Int J Biochem Cell Biol 41:2134-46. 2009
    The dynamic post-translational modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), termed O-GlcNAcylation, is an important mechanism for modulating cellular signaling pathways...
  69. ncbi Dynamic O-GlcNAc modification of nucleocytoplasmic proteins in response to stress. A survival response of mammalian cells
    Natasha E Zachara
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 279:30133-42. 2004
    ..A wide variety of stress stimuli rapidly increase nucleocytoplasmic protein modification by O-linked beta-N-acetylglucosamine (O-GlcNAc), an essential post-translational modification of Ser and Thr residues of metazoans...
  70. ncbi Mapping sites of O-GlcNAc modification using affinity tags for serine and threonine post-translational modifications
    Lance Wells
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21218, USA
    Mol Cell Proteomics 1:791-804. 2002
    ..O-Linked beta-N-acetylglucosamine (O-GlcNAc) is a dynamic nucleocytoplasmic modification more analogous to phosphorylation than to classical ..
  71. pmc O-GLYCBASE version 4.0: a revised database of O-glycosylated proteins
    R Gupta
    Center for Biological Sequence Analysis, The Technical University of Denmark, DK 2800 Lyngby, Denmark
    Nucleic Acids Res 27:370-2. 1999
    ..Sequence logos representing the acceptor specificity patterns for GalNAc, GlcNAc, mannosyl and xylosyl transferases are shown. The O-GLYCBASE database is available through the WWW at http://www.cbs.dtu.dk/databases/OGLYCBASE/..
  72. ncbi A new strategy for identification of N-glycosylated proteins and unambiguous assignment of their glycosylation sites using HILIC enrichment and partial deglycosylation
    Per Hägglund
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    J Proteome Res 3:556-66. 2004
    ..2.1.96). After hydrolysis with these enzymes, a single N-acetylglucosamine (GlcNAc) residue remains linked to the asparagine residue...
  73. pmc Identification of an N-acetylglucosamine transporter that mediates hyphal induction in Candida albicans
    Francisco J Alvarez
    Graduate Program in Genetics, State University of New York, Stony Brook, NY 11794 5222, USA
    Mol Biol Cell 18:965-75. 2007
    The sugar N-acetylglucosamine (GlcNAc) plays an important role in nutrient sensing and cellular regulation in a wide range of organisms from bacteria to humans...
  74. pmc Binding properties of the N-acetylglucosamine and high-mannose N-glycan PP2-A1 phloem lectin in Arabidopsis
    Julie Beneteau
    UR1268 Unité de Recherches Biopolymères Interactions Assemblages, INRA, F 44300 Nantes, France
    Plant Physiol 153:1345-61. 2010
    ..Using a recombinant protein produced in Escherichia coli, we demonstrated binding to N-acetylglucosamine oligomers...
  75. pmc In vivo imaging of Caenorhabditis elegans glycans
    Scott T Laughlin
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    ACS Chem Biol 4:1068-72. 2009
    ..elegans. We treated worms with azidosugar variants of N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc), and N-acetylmannosamine (ManNAc), resulting in the metabolic ..
  76. pmc Protein modification by O-linked GlcNAc reduces angiogenesis by inhibiting Akt activity in endothelial cells
    Bai Luo
    Division of Endocrinology, University of Utah, Salt Lake City, UT 84132, USA
    Arterioscler Thromb Vasc Biol 28:651-7. 2008
    ..O-linked N-acetylglucosamine (O-GlcNAc) modification on protein is the major mechanism mediating the actions of the HBP...
  77. pmc Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins
    Peter M Clark
    Division of Chemistry and Chemical Engineering and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    J Am Chem Soc 130:11576-7. 2008
    ..As such, this strategy enables studies of O-GlcNAc glycosylation that were previously inaccessible and provides a new tool for uncovering the physiological functions of O-GlcNAc...
  78. ncbi Peptidoglycan N-acetylglucosamine deacetylation decreases autolysis in Lactococcus lactis
    Mickael Meyrand
    INRA, Unité de Biochimie Bactérienne, UR477, 78350 Jouy en Josas, France
    Microbiology 153:3275-85. 2007
    The gene xynD (renamed pgdA) of Lactococcus lactis IL1403 was shown to encode a peptidoglycan N-acetylglucosamine deacetylase. Inactivation of pgdA in L...
  79. pmc Retinal O-linked N-acetylglucosamine protein modifications: implications for postnatal retinal vascularization and the pathogenesis of diabetic retinopathy
    Zafer Gurel
    Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792 4673, USA
    Mol Vis 19:1047-59. 2013
    ..Uridine diphosphate N-acetylglucosamine (GlcNAc) is the product of the hexosamine biosynthetic pathway and the substrate for O-linked GlcNAc (O-..
  80. pmc Protein O-GlcNAcylation: a new signaling paradigm for the cardiovascular system
    Boglarka Laczy
    Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294 0007, USA
    Am J Physiol Heart Circ Physiol 296:H13-28. 2009
    ..residues of nuclear and cytoplasmic proteins by the O-linked attachment of the monosaccharide beta-N-acetylglucosamine (O-GlcNAc) is a highly dynamic and ubiquitous protein modification...
  81. pmc Human YKL-39 is a pseudo-chitinase with retained chitooligosaccharide-binding properties
    Marianne Schimpl
    Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 446:149-57. 2012
    ..A glycan screen identified oligomers of N-acetylglucosamine as preferred binding partners...
  82. ncbi Increasing O-GlcNAc slows neurodegeneration and stabilizes tau against aggregation
    Scott A Yuzwa
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada
    Nat Chem Biol 8:393-9. 2012
    ..Tau is modified by O-linked N-acetylglucosamine (O-GlcNAc), and O-GlcNAc can influence tau phosphorylation in certain cases...
  83. pmc Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase
    Giulia Bandini
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Mol Microbiol 85:513-34. 2012
    The enzymes phosphomannomutase (PMM), phospho-N-acetylglucosamine mutase (PAGM) and phosphoglucomutase (PGM) reversibly catalyse the transfer of phosphate between the C6 and C1 hydroxyl groups of mannose, N-acetylglucosamine and glucose ..
  84. ncbi The superhelical TPR-repeat domain of O-linked GlcNAc transferase exhibits structural similarities to importin alpha
    Martin Jinek
    Structural and Computational Biology, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Struct Mol Biol 11:1001-7. 2004
    Addition of N-acetylglucosamine (GlcNAc) is a ubiquitous form of intracellular glycosylation catalyzed by the conserved O-linked GlcNAc transferase (OGT)...
  85. ncbi The transcription factor PDX-1 is post-translationally modified by O-linked N-acetylglucosamine and this modification is correlated with its DNA binding activity and insulin secretion in min6 beta-cells
    Yuan Gao
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 2185, USA
    Arch Biochem Biophys 415:155-63. 2003
    ..cells and native Min6 cells and demonstrated that the protein is posttranslationally modified by O-linked N-acetylglucosamine (O-GlcNAc)...
  86. pmc Identification and regulation of the N-acetylglucosamine utilization pathway of the plant pathogenic bacterium Xanthomonas campestris pv. campestris
    Alice Boulanger
    Laboratoire des Interactions Plantes Micro organismes, UMR CNRS INRA 2594 441, F 31320 Castanet Tolosan, France
    J Bacteriol 192:1487-97. 2010
    ..campestris pv. campestris CUT system involved in the import and catabolism of N-acetylglucosamine (GlcNAc)...
  87. ncbi N-Acetylglucosamine-dependent biofilm formation in Pectobacterium atrosepticum is cryptic and activated by elevated c-di-GMP levels
    Daniel Pérez-Mendoza
    Department of Biochemistry, University of Cambridge, Cambridge, UK
    Microbiology 157:3340-8. 2011
    ..A modest but consistent increase in pga transcript levels was associated with high intracellular levels of c-di-GMP. Our results indicate tight control of PGA-dependent biofilm formation by c-di-GMP in Pba...
  88. pmc O-GlcNAcylation contributes to the vascular effects of ET-1 via activation of the RhoA/Rho-kinase pathway
    Victor V Lima
    Department of Physiology, Medical College of Georgia, 1120 Fifteenth Street, CA 3141, Augusta, GA 30912 3000, USA
    Cardiovasc Res 89:614-22. 2011
    Glycosylation with β-N-acetylglucosamine (O-GlcNAcylation) is one of the most complex post-translational modifications. The cycling of O-GlcNAc is controlled by two enzymes: UDP-NAc transferase (OGT) and O-GlcNAcase (OGA)...
  89. pmc O-linked beta-N-acetylglucosamine during hyperglycemia exerts both anti-inflammatory and pro-oxidative properties in the endothelial system
    Angana Gupta Rajapakse
    Vascular Biology, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg, Switzerland
    Oxid Med Cell Longev 2:172-5. 2009
    Elevated cellular levels of protein O-linked beta-N-acetylglucosamine (O-GlcNAc) through hexosamine biosynthesis pathway (HBP) are suggested to contribute to cardiovascular adverse effects under chronic hyperglycemic condition associated ..
  90. pmc O-linked beta-N-acetylglucosamine (O-GlcNAc) regulates stress-induced heat shock protein expression in a GSK-3beta-dependent manner
    Zahra Kazemi
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 285:39096-107. 2010
    To investigate the mechanisms by which O-linked β-N-acetylglucosamine modification of nucleocytoplasmic proteins (O-GlcNAc) confers stress tolerance to multiple forms of cellular injury, we explored the role(s) of O-GlcNAc in the ..
  91. ncbi O-GlcNAcylation increases non-amyloidogenic processing of the amyloid-β precursor protein (APP)
    Kristin T Jacobsen
    Department of Neurochemistry, Stockholm University, SE 10691 Stockholm, Sweden
    Biochem Biophys Res Commun 404:882-6. 2011
    ..Our results implicate O-GlcNAcylation as a potential therapeutic target for Alzheimer's disease...
  92. ncbi Molecular cloning and characterization of mouse ficolin-A
    Y Fujimori
    Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan
    Biochem Biophys Res Commun 244:796-800. 1998
    ..Immunoblot analysis using an anti-mouse ficolin-A antiserum showed that mouse ficolin-A is a plasma protein with binding activity to elastin and GlcNAc...
  93. ncbi Structure and function of N-acetylglucosamine kinase. Identification of two active site cysteines
    Markus Berger
    Institut fur Molekularbiologie und Biochemie, Freie Universitat Berlin, Arnimallee 22, D 14195 Berlin Dahlem, Germany
    Eur J Biochem 269:4212-8. 2002
    N-Acetylglucosamine is a major component of complex carbohydrates...
  94. pmc Convergent pathways for utilization of the amino sugars N-acetylglucosamine, N-acetylmannosamine, and N-acetylneuraminic acid by Escherichia coli
    J Plumbridge
    Institut de Biologie Physico Chimique UPR9073, 75005 Paris, France
    J Bacteriol 181:47-54. 1999
    N-Acetylglucosamine (GlcNAc) and N-acetylneuraminic acid (NANA) are good carbon sources for Escherichia coli K-12, whereas N-acetylmannosamine (ManNAc) is metabolized very slowly...
  95. ncbi The tumor suppressor EXT-like gene EXTL2 encodes an alpha1, 4-N-acetylhexosaminyltransferase that transfers N-acetylgalactosamine and N-acetylglucosamine to the common glycosaminoglycan-protein linkage region. The key enzyme for the chain initiation of he
    H Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 274:13933-7. 1999
    ..Interestingly, the enzyme also catalyzed the transfer of N-acetylglucosamine (GlcNAc) from UDP-[3H]GlcNAc to GlcAbeta1-3Galbeta1-O-naphthalenemethanol, which was the acceptor substrate ..
  96. ncbi Targeting of macrophage galactose-type C-type lectin (MGL) induces DC signaling and activation
    Chiara Napoletano
    Department of Experimental Medicine, Sapienza University, Rome, Italy
    Eur J Immunol 42:936-45. 2012
    ..These results demonstrate that the targeting of MGL receptor on human DCs has an adjuvant effect and that this strategy can be used to design novel anticancer vaccines...
  97. pmc O-linked N-acetylglucosamine modification on CCAAT enhancer-binding protein beta: role during adipocyte differentiation
    Xi Li
    Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University Shanghai Medical College, Shanghai 200032, China
    J Biol Chem 284:19248-54. 2009
    ..Mutation of both Ser(180) and Ser(181) to Ala significantly increase the transcriptional activity of C/EBPbeta. These data suggest that GlcNAcylation regulates both the phosphorylation and DNA binding activity of C/EBPbeta...
  98. ncbi N-Acetylglucosamine 6-O-sulfotransferase-1 is required for brain keratan sulfate biosynthesis and glial scar formation after brain injury
    Haoqian Zhang
    Department of Biochemistry, Institute for Advanced Research, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Glycobiology 16:702-10. 2006
    ..composed of repeating disaccharide units with sulfate residues at the C6 positions of galactose and N-acetylglucosamine (GlcNAc)...
  99. ncbi Phosphoenolpyruvate phosphotransferase system and N-acetylglucosamine metabolism in Bacillus sphaericus
    Alejandro F Alice
    Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
    Microbiology 149:1687-98. 2003
    ..has some unusual features, as they are co-transcribed with genes encoding metabolic enzymes related to N-acetylglucosamine (GlcNAc) catabolism (nagA, nagB and an undetermined orf2)...
  100. ncbi Different regions of Mlc and NagC, homologous transcriptional repressors controlling expression of the glucose and N-acetylglucosamine phosphotransferase systems in Escherichia coli, are required for inducer signal recognition
    Carole Pennetier
    Institut de Biologie Physico Chimique UPR9073 CNRS, 13 rue Pierre et Marie Curie, 75005 Paris, France
    Mol Microbiol 67:364-77. 2008
    ....
  101. pmc O-GLcNAc post-translational modifications regulate the entry of neurons into an axon branching program
    Herb Francisco
    Department of Neurobiology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA
    Dev Neurobiol 69:162-73. 2009
    ..cytosolic and nuclear proteins are post-translationally modified by the reversible addition of O-linked N-acetylglucosamine (O-GlcNAc) on serines and threonines...

Research Grants64

  1. Protein Modifications and Unfolded Protein Response in Diabetic Embryopathy
    Zhiyong Zhao; Fiscal Year: 2013
    ..embryo, augmenting the hexosamine biosynthetic pathway (HBP) to produce high levels of uridine diphosphate N-acetylglucosamine (UDP-GlcNAc). Catalyzed by O-linked-GlcNAc transferase (OGT), a large number of proteins are O- GlcNAcylated...
  2. Environmental Chitin Exposure in the Pathogenesis of Asthma
    Rene M Roy; Fiscal Year: 2012
    ..Chitin, which is the second most abundant polymer on our planet, is a linear polymer of N- acetylglucosamine. In recent work, exposure of mice to chitin particles induced the accumulation of immune cells associated ..
  3. Photocleavable Bead Technology for Glycomics
    Mark Lim; Fiscal Year: 2013
    ..builds on the recently reported use of AmberGen's photocleavable linkers to identify O-linked beta- N-acetylglucosamine (O-GlcNAc) protein modifications in cells, tissues and other biospecimens...
  4. Regulation of IGF-1 and Insulin Signaling by O-GlcNAc Glycosylation
    LAUREN ELIZABETH BALL; Fiscal Year: 2013
    ..IRS-1 and IRS-2 are also glycosylated at Ser/Thr residues by N-acetylglucosamine (O-GlcNAc)...
  5. Development and validation of a chitin assay for environmental samples
    CHRISTINE ANNE ROGERS; Fiscal Year: 2012
    ....
  6. Bordetella Biofilms and Pathogenesis
    RAJENDAR K DEORA; Fiscal Year: 2011
    ..Bps is similar to PGA/PIA/PNAG (poly-2-1,6-N- acetylglucosamine) polysaccharides of other bacteria including a number of pathogens...
  7. Modulation of Autoimmune Diabetes by N-acetyl-D-glucosamine Specific B cells
    BRIAN L DIZON; Fiscal Year: 2010
    ..Understanding the mechanisms involved in this link may provide insight on new strategies for earlier diagnosis, treatments, and prevention of T1D. ..
  8. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Warren Hart; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The modification of proteins by [unreadable]-N-Acetylglucosamine (O-GlcNAc) has extensive crosstalk with Ser (Thr)-protein phosphorylation to regulate signaling and gene expression in response to ..
  9. N-acetylglucosamine signaling in fungi
    James B Konopka; Fiscal Year: 2012
    ..In particular, studies will focus on N-acetylglucosamine (GlcNAc)...
  10. Structural and Biochemical Studies of LpxC Inhibition
    Pei Zhou; Fiscal Year: 2012
    ..The committed step of lipid A biosynthesis is catalyzed by UDP-3-O-(acyl)-N- acetylglucosamine deacetylase (LpxC)...
  11. The Western diet and O-GlcNAc modification of chromatin proteins
    SUSAN ANNE MARSH; Fiscal Year: 2011
    ..The post- translational O-linked attachment of a -N-acetylglucosamine (O-GlcNAc) sugar to serine/threonine residues of proteins is an important mediator of signal transduction, ..
  12. O-GlcNAc Modification in Metabolic Homeostasis
    Xiaoyong Yang; Fiscal Year: 2013
    ..pathway leads to the posttranslational modification of cytoplasmic and nuclear proteins by O-linked 2-N-acetylglucosamine (O-GlcNAc)...
  13. GENETIC ANALYSIS OF SYNAPSE FORMATION AND FUNCTION
    Kendal Broadie; Fiscal Year: 2013
    ..of which are regulated by 5) GlcNAc Transferase 1 (Mgat1) and 6) secreted Mind-the-Gap (Mtg) lectin for N-acetylglucosamine (GlcNAc)-proteoglycans;and 7) secreted matrix metalloproteinase (MMP) and tissue inhibitor of MMP (TIMP), ..
  14. Hyperglycemia-induced translational control of gene expression in the retina
    Michael D Dennis; Fiscal Year: 2013
    ..The central hypothesis is that the addition of O- linked N-Acetylglucosamine (O-GlcNAcylation) to serine or threonine residues of translation initiation factors mediates a shift from ..
  15. Assembly of the mycobacterial cell wall
    Martin S Pavelka; Fiscal Year: 2012
    ..Mycobacterial PGN polysaccharide is composed of N-acylmuramic acid and N-acetylglucosamine with peptides (L-alanyl (or glycyl)-D-iso-glutaminyl-meso-diaminopimelyl-D-alanyl-D- alanine) attached to ..
  16. O-linked glycosylation and synaptic function in hippocampus
    LORI LYNN contact MCMAHON; Fiscal Year: 2010
    ..The HBP modifies glucose to produce an O-linked N-acetylglucosamine (O-GlcNAc) moiety that can be added to serine/threonine residues...
  17. Collagen I, Hyaluronan, and Aging
    May J Reed; Fiscal Year: 2011
    ..Notably, HA - a simple polymer of the disaccharide D-glucuronic acid/D-N-acetylglucosamine - has differential effects on angiogenesis that are related to its chain length...
  18. The Role of Uromodulin in the Regulation of the Renal Calcium Channel TRPV5
    MATTHIAS TILMANN FLORIAN WOLF; Fiscal Year: 2013
    ..TRPV5 is regulated by Klotho, an anti-aging protein. Klotho functions as a sialidase, exposing galactose-N-acetylglucosamine at N-glycosylated TRPV5 channels, which then bind to galectin-1, a ubiquitous lectin...
  19. Innate Immune Sensing of Bacterial Sugars
    David M Underhill; Fiscal Year: 2013
    ..We have shown that upon degradation of peptidoglycan in phagosomes a component sugar, N-acetylglucosamine, is freed up and transported into the cytosol where it interacts with hexokinase causing release of the ..
  20. HEXOSAMINES, GLUCOSE TOXICITY AND INSULIN RESISTANCE
    Donald A McClain; Fiscal Year: 2013
    ..Modification of cytosolic, nuclear, and mitochondrial proteins on serine and threonine residues by N-acetylglucosamine (O-GlcNAc) provides a mechanism by which glucose flux rates in the cell can feed back to regulate the ..
  21. Chimeric IgG/A Tumor Immunotherapy
    KEITH WYCOFF; Fiscal Year: 2013
    ..In particular, the absence of core ?1-6)-Fucose and the presence of a bisecting N- acetylglucosamine (GlcNAc) residue each enhance Fc binding to Fc?RIIIa...
  22. Defining the Molecular Mechanisms by Which O-GlcNAc Mediates Cardioprotection
    Natasha Elizabeth Zachara; Fiscal Year: 2012
    ..applicant): Recently, the modification of nuclear, mitochondrial, and cytoplasmic proteins by O-linked 2-N- acetylglucosamine (O-GlcNAc) has emerged as a novel regulator of the stress response and cell survival...
  23. STRUCTURE, BIOSYNTHESIS &FUNCTION OF GLYCOPROTEINS
    Stuart A Kornfeld; Fiscal Year: 2013
    ..of mannose residues on the high mannose glycans of the acid hydrolases by UDP-GlcNAc: lysosomal enzyme N-acetylglucosamine-1- phosphotransferase (Ptase). This transferase is an alpha2 beta2 gamma2 hexameric protein...
  24. Microfluidic devices for determining dynamics of islets of Langerhans
    MICHAEL GABRIEL ROPER; Fiscal Year: 2013
    ..ROS production;3) Determine the effect of oscillatory glucose levels on protein modification by O-linked N- acetylglucosamine (O-GlcNAc)...
  25. O-GlcNAcylation and Hippocampal Synaptic Plasticity
    LORI LYNN MCMAHON; Fiscal Year: 2013
    ..The HBP modifies glucose to produce an O-linked N-acetylglucosamine (O-GlcNAc) moiety that can be added to serine/threonine residues of proteins in a highly dynamic and ..
  26. Immunostimulatory properties of chitin and chitosan
    CHARLES ALLEN SPECHT; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Chitin, a homopolymer consisting of 2-1,4-linked repeating units of N-acetylglucosamine, is the second most abundant polysaccharide in nature...
  27. Site-specific O-GlcNAc MAbs: New Tools for Glycoproteomics
    Ron Orlando; Fiscal Year: 2013
    ..We feel that these will have far reaching implications in disease research. ..
  28. Studies of Protein Glycosylation
    Carolyn R Bertozzi; Fiscal Year: 2013
    ..GalNAc) residue, and O-GlcNAcylation in which Ser/Thr residues are modified with a single 2-N-acetylglucosamine (GlcNAc) residue. Changes in mucin-type O-glycoprotein expression have been associated with cancer...
  29. Mass Spectrometry Tools in Pursuit of Salivary Biomarkers of Sjogren's Syndrome
    Steven C Hall; Fiscal Year: 2013
    ..high levels of the unusual carbohydrate structure, sialyl Lewis x with sulfation on the 6-position of N-acetylglucosamine, which mediates rolling and tethering during leukocyte recruitment from the blood...
  30. Chemical Approaches to Understand the Roles of O-GlcNAc Glycosylation in Biology
    Linda C Hsieh-Wilson; Fiscal Year: 2013
    ..we developed chemical methods for the rapid, sensitive detection and study of O-linked [unreadable]-D-N-acetylglucosamine (O-GlcNAc) glycosylation...
  31. O-GlyNAcylation: Novel Mechanism of Estrogen-Induced Vasoprotection
    Suzanne Oparil; Fiscal Year: 2012
    Project Summary/Abstract We have made the seminal observation that O-linked [unreadable]-N-acetylglucosamine (O-GlcNAc) modification of proteins induced by three distinct and independent stimuli, 17-[unreadable] estradiol (E2), ..
  32. O-GlcNAcylation of tau: a link between glucose metabolism and neurodegeneration
    Cheng Xin Gong; Fiscal Year: 2010
    ..the nature and functional role of tau O-GlcNAcylation [a unique type of O-glycosylation by which beta-N-acetylglucosamine (GlcNAc) is linked to serine or threonine residues of proteins] and to uncover the mechanism by which ..
  33. Biological function of keratan sulfate glycosaminoglycan for corneal extracellula
    Tomoya O Akama; Fiscal Year: 2010
    ..7, ~1 ,4-galactosyltransferase 4, KS galactose 6-0 sulfotransferase and corneal N-acetylglucosamine 6-0 sulfotransferase, are required for KS-GAG production in vitro as well as in vivo...
  34. The role of N-glycosylation on beta-sheet protein folding energetics
    JOSHUA L PRICE; Fiscal Year: 2010
    ..This work could ultimately shed light on the relationship between protein glycosylation and protein secretion efficiency, which is related to human lysosomal storage diseases. ..
  35. Chemical and enzymatic synthesis of multi-domain heparin mimetics
    TIMOTHY OLEARY; Fiscal Year: 2013
    ..Both are based on a repeating unit of [unreadable]-uronic acid linked to [unreadable]-N-acetylglucosamine. In the cell these polysaccharides are modified by a series of enzymes to yield heterogeneous, highly ..
  36. Dissecting the roles of protein O-GlcNAcylation in Toxoplasma gondii
    Peng Wu; Fiscal Year: 2013
    ..gondii. OGT adds a single [unreadable]-N-acetylglucosamine to serine or threonine residues of intracellular proteins...
  37. Role of Mammalian Chitinases in Inflammatory Bowel Disease
    Emiko Mizoguchi; Fiscal Year: 2013
    ..In Aim-III, we will define a prophylactic effect of orally administered chitin, a polymer of N-acetylglucosamine and substrate for CHI3L1, in animal models of acute and chronic colitis...
  38. SBIR TOPIC 294 PHASE I - DEVELOPMENT OF GLYCOSYLATION-SPECIFIC RESEARCH REAGENTS
    Hyesook Kim; Fiscal Year: 2011
    Protein O-GlcNAcylation (N-acetylglucosamine modification), which decreases phosphorylation, plays a critical role in cell-cycle regulation, apoptosis and signal transduction. So far, ~120 O-GlcNAcylated human proteins have been found...
  39. Uncovering the mechanism of OGT substrate recognition and membrane localization
    RODRIGO FERMIN ORTIZ-MEOZ; Fiscal Year: 2013
    ..into a pathway that leads to the stimulation of an enzyme which is responsible for the addition of 2-N-acetylglucosamine (O-GlcNAc) to over 1,000 proteins in the cell...
  40. New chemoenzymatic methods for synthesizing complex carbohydrates
    Xi Chen; Fiscal Year: 2013
    ..Four specific aims are 1) chemical synthesis of N-acetylglucosamine (GlcNAc) and uronic acid derivatives as monosaccharide precursors;2) synthesis of UDP-GlcNAc, UDP-uronic ..
  41. Sialic acid has putative signaling roles in muscle disorders of aging
    Nam Pham; Fiscal Year: 2013
    ..Mutations in UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase gene (GNE), which encodes the enzyme that catalyzes the first ..
  42. Anti-staphylococcal activities of A. actinomycetemcomitans dispersin B
    Jeffrey B Kaplan; Fiscal Year: 2010
    ..the most common device-associated pathogens, contains a sticky polysaccharide named PNAG (poly-N-acetylglucosamine) that mediates surface attachment, intercellular adhesion, biocide resistance and immune evasion...
  43. Roles of Glycoslyation in Notch Signaling
    PAMELA M STANLEY; Fiscal Year: 2013
    ..identify individual and complementary functions of Lfng, Mfng and Rfng glycosyltransferases that transfer N-acetylglucosamine (GlcNAc) to O-fucose on Notch, and thereby regulate Notch signaling...
  44. HTS assay development for inhibitors of sickle cell
    PAUL FRENETTE; Fiscal Year: 2006
    ..catalyze the formation of an alpha anomeric glycosidic bond between carbon 1 of the fucose and carbon 3 of N-acetylglucosamine. We have developed an ELISA-based assay to evaluate rapidly the FucT activity in cell lysates...
  45. O-GlcNAc Signaling in Heart Failure
    Steven P Jones; Fiscal Year: 2012
    ..Pathway, which forms the monosaccharide donor for the post-translational modification known as O-linked-2-N- acetylglucosamine (O-GlcNAc)...
  46. CHITIN BIOSYNTHESIS AND THE MOSQUITO PERITROPHIC MATRIX
    Bruce Christensen; Fiscal Year: 2007
    ..N-acetyltransferase (GNAT), phosphoacetyl glucosamine mutase (AGM), and uridine diphosphate-N-acetylglucosamine pyrophophrylase (UAP) are also involved in this pathway but have never been cloned or characterized in any ..
  47. 'Immunity of Schistosome Glycoconjugates'
    Richard Cummings; Fiscal Year: 2001
    ..five major antigenic glycans in bi- and trisaccharide sequences containing fucose, galactose, xylose, N-acetylglucosamine and N-acetylgalactosamine...
  48. Vibrio Cholerae Colonization of Chitin Surfaces
    Gary Schoolnik; Fiscal Year: 2007
    ..Chitin is an insoluble polymer of N-acetylglucosamine; through the secretion of chitinases, V...
  49. STRUCTURAL ANALYSIS OF GLYCOCONJUGATE METABOLISM
    Patrick Van Roey; Fiscal Year: 2000
    ..Endoglycosidases are glycohydrolases that cleave the glycosidic link between the two N-acetylglucosamine residues in the core of the oligosaccharide chain...
  50. Structure/function studies of biofilm agents from Aa
    Narayanan Ramasubbu; Fiscal Year: 2009
    ..Specific Aim 2. Identify structural components that contribute to the deacetylase activity of PgaB. Specific Aim 3. Demonstrate that the state of acetylation of PGA contributes to the biofilm formation in Aa. ..
  51. REGULATION OF GLCNAC-PI DEACETYLATION
    Victoria Stevens; Fiscal Year: 1999
    ..this proposal, we will study the regulation of the second step in GPI biosynthesis, the deacetylation of N-acetylglucosamine-phosphatidylinositol (GlcNAc-PI) to form glucosamine-phosphatidylinositol (GlcN-PI)...
  52. GLUCOSAMINE IN CARTILAGE HOMEOSTASIS AND DEGRADATION
    ALEXANDER SHIKHMAN; Fiscal Year: 2004
    ..Recently, we discovered that glucosamine (GlcN) and its derivative, N-acetylglucosamine (GlcNAc), express a broad range of anti-inflammatory activities in cultured human articular chondrocytes ..
  53. INNOVATIVE INTRAVESICAL TREATMENT APPROACH FOR IC
    Veer Bhavanandan; Fiscal Year: 2001
    ..Preliminary studies have demonstrated the presence of galactose- and N-acetylglucosamine-binding lectins in both rabbit and human bladders...
  54. Study Of Mycobacterial Galactan Biosynthesis By Chemoenzymatic Substrate Assembly
    Mario A Martinez Farias; Fiscal Year: 2013
    ..assembly takes place on an initiator sequence consisting of the disaccharide rhamnosyl (Rha)-?-(1->3)-N-acetylglucosamine (GlcNAc) pyrophosphate linked to a C50 (decaprenyl) lipid...
  55. MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASES
    JON THORSON; Fiscal Year: 2002
    ....
  56. 'Immunity of Schistosome Glycoconjugates'
    Richard Cummings; Fiscal Year: 2003
    ..These antigenic glycans occur in di- and trisaccharide sequences containing fucose, galactose, xylose, N-acetylglucosamine and N-acetylgalactosamine...
  57. CHARACTERIZATION OF MICROBIAL LECTINS
    Patricia Murray; Fiscal Year: 1992
    ..to systematically screen many oral bacterial strains to identify binding sites for sialic acid, galactose, N-acetylglucosamine, and fucose using glycopeptide analogues containing the appropriate terminal sugar...
  58. GIARDIA ENCYSTMENT: CONTROL OF GA1NAC SYNTHESIS
    Edward Jarroll; Fiscal Year: 2000
    ..GPI, glucosamine 6-phosphate N-acetylase (GlcNPA), phosphoacetylglucosamine mutase (PAG1cNM), UDP-N- acetylglucosamine pyrophosphorylase (UDP-GlcNAcPP), and UDP-N- acetylglucosamine 4' epimerase (UDP-GlcNAcE)...
  59. OGT Overexpression in Women with Lupus
    Bruce Richardson; Fiscal Year: 2009
    ..We surveyed methylation sensitive T cell genes, and identified O-linked N-acetylglucosamine transferase (OGT) as another X-linked gene overexpressed in demethylated female T cells...
  60. MOLECULAR CONTROL OF N-GLYCOSYLATION IN DEVELOPMENT
    MARIA KUKURUZINSKA; Fiscal Year: 1990
    ..is regulated at the very first step in the pathway catalyzed by the tunicamycin sensitive enzyme, N- acetylglucosamine-1-phosphate (ALG7) transferase...
  61. PERIODONTAL DISEASE:ROLE OF ABERRANT Ig GLYCOSYLATION
    Jiri Mestecky; Fiscal Year: 2006
    ..Deficiency of Gal residues renders such molecules pathogenic due to the fact that terminal N-acetylglucosamine residues, normally covered by Gal, became exposed and are recognized by the ubiquitous mannose-binding ..
  62. GLYCOSYLATION MECHANISMS
    CARLOS HIRSCHBERG; Fiscal Year: 2009
    ..elegans'transportersfor UDP-Galactose/UDP-N-Acetylglucosamine discovered in our laboratory...
  63. STRUCTURES OF NEURECTODERM TUMOR GLYCOPROTEINS
    MARY GLICK; Fiscal Year: 1992
    ..of human neuroblastoma cells contain a high proportion of fucosyl residues linked alpha 1 leads to 3 to N- acetylglucosamine (Fuc alpha 1 leads to 3G1cNAc) on the oligosaccharide antennae...
  64. Role of HSulf-1 Loss in Apoptosis and Drug Resistance
    Viji Shridhar; Fiscal Year: 2007
    ..sulfatase activity and diminishes the sulfation of heparin sulfate proteoglycans (HSPGs), specifically the N-acetylglucosamine residue of glycosaminoglycans...