acetylglucosamine

Summary

Summary: The N-acetyl derivative of glucosamine.

Top Publications

  1. ncbi Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteins
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205 2185, USA
    Nature 446:1017-22. 2007
  2. pmc Characterization of O-acetylation of N-acetylglucosamine: a novel structural variation of bacterial peptidoglycan
    Elvis Bernard
    INRA, UMR1319 MICALIS, F 78350 Jouy en Josas, France
    J Biol Chem 286:23950-8. 2011
  3. pmc Hyperglycemia inhibits endothelial nitric oxide synthase activity by posttranslational modification at the Akt site
    X L Du
    Diabetes Research Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Clin Invest 108:1341-8. 2001
  4. pmc O-GlcNAc signaling: a metabolic link between diabetes and cancer?
    C Slawson
    The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725N Wolfe St Baltimore, MD 21205, USA
    Trends Biochem Sci 35:547-55. 2010
  5. pmc Differential roles of poly-N-acetylglucosamine surface polysaccharide and extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis biofilms
    Era A Izano
    Medical Science Building, Room C 636, 185 S Orange Ave, Newark, NJ 07103, USA
    Appl Environ Microbiol 74:470-6. 2008
  6. pmc Cross talk between O-GlcNAcylation and phosphorylation: roles in signaling, transcription, and chronic disease
    Gerald W Hart
    Departments of Biological Chemistry and Pediatrics, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205
    Annu Rev Biochem 80:825-58. 2011
  7. ncbi Essential role of the glycosyltransferase sxc/Ogt in polycomb repression
    Maria Cristina Gambetta
    Gene Expression Programme, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Science 325:93-6. 2009
  8. pmc O-linked beta-N-acetylglucosamine (O-GlcNAc): Extensive crosstalk with phosphorylation to regulate signaling and transcription in response to nutrients and stress
    Chutikarn Butkinaree
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Biochim Biophys Acta 1800:96-106. 2010
  9. pmc Immunochemical properties of the staphylococcal poly-N-acetylglucosamine surface polysaccharide
    Tomas Maira-Litran
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts, USA
    Infect Immun 70:4433-40. 2002
  10. pmc Colistin-resistant, lipopolysaccharide-deficient Acinetobacter baumannii responds to lipopolysaccharide loss through increased expression of genes involved in the synthesis and transport of lipoproteins, phospholipids, and poly-β-1,6-N-acetylglucosamine
    Rebekah Henry
    Department of Microbiology, Monash University, Clayton, Australia
    Antimicrob Agents Chemother 56:59-69. 2012

Research Grants

Detail Information

Publications259 found, 100 shown here

  1. ncbi Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteins
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205 2185, USA
    Nature 446:1017-22. 2007
    All animals and plants dynamically attach and remove O-linked beta-N-acetylglucosamine (O-GlcNAc) at serine and threonine residues on myriad nuclear and cytoplasmic proteins...
  2. pmc Characterization of O-acetylation of N-acetylglucosamine: a novel structural variation of bacterial peptidoglycan
    Elvis Bernard
    INRA, UMR1319 MICALIS, F 78350 Jouy en Josas, France
    J Biol Chem 286:23950-8. 2011
    ..We report here the presence of O-acetylation on N-acetylglucosamine (GlcNAc) in Lactobacillus plantarum PG. This modification of glycan strands was never described in bacteria...
  3. pmc Hyperglycemia inhibits endothelial nitric oxide synthase activity by posttranslational modification at the Akt site
    X L Du
    Diabetes Research Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Clin Invest 108:1341-8. 2001
    ..Since hyperglycemia-induced mitochondrial superoxide overproduction increases O-linked N-acetylglucosamine modification and decreases O-linked phosphorylation of the transcription factor Sp1, the effect of ..
  4. pmc O-GlcNAc signaling: a metabolic link between diabetes and cancer?
    C Slawson
    The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725N Wolfe St Baltimore, MD 21205, USA
    Trends Biochem Sci 35:547-55. 2010
    O-linked β-N-acetylglucosamine (O-GlcNAc) is a sugar attachment to serine or threonine hydroxyl moieties on nuclear and cytoplasmic proteins...
  5. pmc Differential roles of poly-N-acetylglucosamine surface polysaccharide and extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis biofilms
    Era A Izano
    Medical Science Building, Room C 636, 185 S Orange Ave, Newark, NJ 07103, USA
    Appl Environ Microbiol 74:470-6. 2008
    ..In this study, we investigated the functions of two staphylococcal biofilm matrix polymers: poly-N-acetylglucosamine surface polysaccharide (PNAG) and extracellular DNA (ecDNA)...
  6. pmc Cross talk between O-GlcNAcylation and phosphorylation: roles in signaling, transcription, and chronic disease
    Gerald W Hart
    Departments of Biological Chemistry and Pediatrics, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205
    Annu Rev Biochem 80:825-58. 2011
    O-GlcNAcylation is the addition of β-D-N-acetylglucosamine to serine or threonine residues of nuclear and cytoplasmic proteins...
  7. ncbi Essential role of the glycosyltransferase sxc/Ogt in polycomb repression
    Maria Cristina Gambetta
    Gene Expression Programme, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Science 325:93-6. 2009
    ..super sex combs (sxc) encodes Ogt, the highly conserved glycosyltransferase that catalyzes the addition of N-acetylglucosamine (GlcNAc) to proteins in animals and plants...
  8. pmc O-linked beta-N-acetylglucosamine (O-GlcNAc): Extensive crosstalk with phosphorylation to regulate signaling and transcription in response to nutrients and stress
    Chutikarn Butkinaree
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Biochim Biophys Acta 1800:96-106. 2010
    Since its discovery in the early 1980s, O-linked-beta-N-acetylglucosamine (O-GlcNAc), a single sugar modification on the hydroxyl group of serine or threonine residues, has changed our views of protein glycosylation...
  9. pmc Immunochemical properties of the staphylococcal poly-N-acetylglucosamine surface polysaccharide
    Tomas Maira-Litran
    Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts, USA
    Infect Immun 70:4433-40. 2002
    ..intercellular adhesin (PIA), which is composed of a approximately 28 kDa soluble linear beta(1-6)-linked N-acetylglucosamine. We developed a new method to purify PS/A from S. aureus MN8m, a strain hyperproducing PS/A...
  10. pmc Colistin-resistant, lipopolysaccharide-deficient Acinetobacter baumannii responds to lipopolysaccharide loss through increased expression of genes involved in the synthesis and transport of lipoproteins, phospholipids, and poly-β-1,6-N-acetylglucosamine
    Rebekah Henry
    Department of Microbiology, Monash University, Clayton, Australia
    Antimicrob Agents Chemother 56:59-69. 2012
    ..In addition, genes involved in the synthesis and transport of poly-β-1,6-N-acetylglucosamine (PNAG) also were upregulated, and a corresponding increase in PNAG production was observed...
  11. ncbi Impact of Type 2 diabetes and aging on cardiomyocyte function and O-linked N-acetylglucosamine levels in the heart
    Norbert Fülöp
    Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Am J Physiol Cell Physiol 292:C1370-8. 2007
    Increased levels of O-linked attachment of N-acetylglucosamine (O-GlcNAc) on nucleocytoplasmic proteins are implicated in the development of diabetic cardiomyopathy and are regulated by O-GlcNAc transferase (OGT) expression and its ..
  12. ncbi The hexosamine signaling pathway: deciphering the "O-GlcNAc code"
    Dona C Love
    Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892, USA
    Sci STKE 2005:re13. 2005
    A dynamic cycle of addition and removal of O-linked N-acetylglucosamine (O-GlcNAc) at serine and threonine residues is emerging as a key regulator of nuclear and cytoplasmic protein activity...
  13. pmc O-GlcNAcylation regulates phosphorylation of tau: a mechanism involved in Alzheimer's disease
    Fei Liu
    Department of Neurochemistry, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA
    Proc Natl Acad Sci U S A 101:10804-9. 2004
    ..tau was modified by O-GlcNAcylation, a type of protein O-glycosylation by which the monosaccharide beta-N-acetylglucosamine (GlcNAc) attaches to serine/threonine residues via an O-linked glycosidic bond...
  14. ncbi Nutrient sensor O-GlcNAc transferase regulates breast cancer tumorigenesis through targeting of the oncogenic transcription factor FoxM1
    S A Caldwell
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA, USA
    Oncogene 29:2831-42. 2010
    ..a cell's glucose enters the hexosamine biosynthetic pathway (HBP), which regulates levels of O-linked beta-N-acetylglucosamine (O-GlcNAc), a carbohydrate posttranslational modification of diverse nuclear and cytosolic proteins...
  15. ncbi Phosphoinositide signalling links O-GlcNAc transferase to insulin resistance
    Xiaoyong Yang
    Howard Hughes Medical Institute and Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nature 451:964-9. 2008
    ..pathway leads to the post-translational modification of cytoplasmic and nuclear proteins by O-linked beta-N-acetylglucosamine (O-GlcNAc)...
  16. pmc O-GlcNAc regulates FoxO activation in response to glucose
    Michael P Housley
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 283:16283-92. 2008
    ..are well established, but FoxOs have not been studied in the context of the glucose-derived O-linked beta-N-acetylglucosamine (O-GlcNAc) modification. Here we show that O-GlcNAc on hepatic FoxO1 is increased in diabetes...
  17. pmc Metabolic cross-talk allows labeling of O-linked beta-N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway
    Michael Boyce
    Departments of Chemistry and Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 108:3141-6. 2011
    Hundreds of mammalian nuclear and cytoplasmic proteins are reversibly glycosylated by O-linked β-N-acetylglucosamine (O-GlcNAc) to regulate their function, localization, and stability...
  18. doi Dysregulation of the nutrient/stress sensor O-GlcNAcylation is involved in the etiology of cardiovascular disorders, type-2 diabetes and Alzheimer's disease
    Tony Lefebvre
    CNRS UMR 8576, Unit of Structural and Functional Glycobiology, IFR 147, University of Lille 1, Villeneuve d Ascq, France
    Biochim Biophys Acta 1800:67-79. 2010
    ..Lastly, the finding that AD may correspond to a type-3 diabetes offers new perspectives into the knowledge of the neuropathology and into the search for new therapeutic avenues...
  19. pmc N-acetylglucosamine induces white to opaque switching, a mating prerequisite in Candida albicans
    Guanghua Huang
    Department of Biology, The University of Iowa, Iowa City, Iowa, United States of America
    PLoS Pathog 6:e1000806. 2010
    ..Here, we demonstrate that a second signal, N-acetylglucosamine (GlcNAc), a monosaccharide produced primarily by gastrointestinal tract bacteria, also serves as a potent ..
  20. pmc Cross-talk between GlcNAcylation and phosphorylation: roles in insulin resistance and glucose toxicity
    Ronald J Copeland
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205 2185, USA
    Am J Physiol Endocrinol Metab 295:E17-28. 2008
    O-linked beta-N-acetylglucosamine (O-GlcNAc) is a dynamic posttranslational modification that, analogous to phosphorylation, cycles on and off serine and/or threonine hydroxyl groups...
  21. pmc Beta-N-acetylglucosamine (O-GlcNAc) is part of the histone code
    Kaoru Sakabe
    Department of Biological Chemistry, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:19915-20. 2010
    Dynamic posttranslational modification of serine and threonine residues of nucleocytoplasmic proteins by β-N-acetylglucosamine (O-GlcNAc) is a regulator of cellular processes such as transcription, signaling, and protein-protein ..
  22. pmc The hexosamine signaling pathway: O-GlcNAc cycling in feast or famine
    John A Hanover
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1800:80-95. 2010
    ..It will emphasize the impact O-GlcNAcylation has upon signaling pathways that may be become deregulated in diseases of the immune system, diabetes mellitus, cancer, cardiovascular disease, and neurodegenerative diseases...
  23. ncbi Site-specific interplay between O-GlcNAcylation and phosphorylation in cellular regulation
    Ping Hu
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, MD 21205 2185, USA
    FEBS Lett 584:2526-38. 2010
    Ser(Thr)-O-linked beta-N-acetylglucosamine (O-GlcNAc) is a ubiquitous modification of nucleocytoplasmic proteins...
  24. ncbi Characterization of a mouse monoclonal antibody specific for O-linked N-acetylglucosamine
    F I Comer
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205-2185, USA
    Anal Biochem 293:169-77. 2001
    beta-O-linked N-acetylglucosamine (O-GlcNAc) is an abundant posttranslational modification of resident nuclear and cytoplasmic proteins in eukaryotes...
  25. doi Analysis of the human cancer glycome identifies a novel group of tumor-associated N-acetylglucosamine glycan antigens
    Tero Satomaa
    Glykos Finland Ltd, Helsinki, Finland
    Cancer Res 69:5811-9. 2009
    ..In conclusion, the identified terminal GlcNAc glycans should be recognized as a novel class of tumor markers...
  26. pmc Identification and regulation of the N-acetylglucosamine utilization pathway of the plant pathogenic bacterium Xanthomonas campestris pv. campestris
    Alice Boulanger
    Laboratoire des Interactions Plantes Micro organismes, UMR CNRS INRA 2594 441, F 31320 Castanet Tolosan, France
    J Bacteriol 192:1487-97. 2010
    ..campestris pv. campestris CUT system involved in the import and catabolism of N-acetylglucosamine (GlcNAc)...
  27. ncbi Molecular cloning and characterization of mouse ficolin-A
    Y Fujimori
    Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan
    Biochem Biophys Res Commun 244:796-800. 1998
    ..Immunoblot analysis using an anti-mouse ficolin-A antiserum showed that mouse ficolin-A is a plasma protein with binding activity to elastin and GlcNAc...
  28. pmc O-GlcNAc signaling in the cardiovascular system
    Gladys A Ngoh
    Institute of Molecular Cardiology, University of Louisville, 580 South Preston St, 404C, Baxter II 404C, Louisville, KY 40202, USA
    Circ Res 107:171-85. 2010
    ..Known as the beta-O-linkage of N-acetylglucosamine (ie, O-GlcNAc) to cellular proteins, this unique monosaccharide is involved in a diverse array of ..
  29. ncbi L-ficolin is a pattern recognition molecule specific for acetyl groups
    Anders Krarup
    Institute of Medical Microbiology and Immunology, University of Aarhus, 8000 Aarhus, Denmark
    J Biol Chem 279:47513-9. 2004
    ..the selectivity of the ficolins through inhibiting the binding to bacteria or to beads coupled with N-acetylglucosamine. The binding of L-ficolin to Streptococcus pneumoniae 11F and the beads was inhibited by N-acetylated sugars ..
  30. pmc Characterization of the opsonic and protective activity against Staphylococcus aureus of fully human monoclonal antibodies specific for the bacterial surface polysaccharide poly-N-acetylglucosamine
    Casie Kelly-Quintos
    Harvard Medical School, 181 Longwood Ave, Boston, MA 02115, USA
    Infect Immun 74:2742-50. 2006
    ....
  31. pmc β-N-Acetylglucosamine (O-GlcNAc) is a novel regulator of mitosis-specific phosphorylations on histone H3
    Jerry J Fong
    Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas 77030 3498, USA
    J Biol Chem 287:12195-203. 2012
    O-Linked β-N-acetylglucosamine, or O-GlcNAc, is a dynamic post-translational modification that cycles on and off serine and threonine residues of nucleocytoplasmic proteins...
  32. ncbi The transcription factor PDX-1 is post-translationally modified by O-linked N-acetylglucosamine and this modification is correlated with its DNA binding activity and insulin secretion in min6 beta-cells
    Yuan Gao
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 2185, USA
    Arch Biochem Biophys 415:155-63. 2003
    ..cells and native Min6 cells and demonstrated that the protein is posttranslationally modified by O-linked N-acetylglucosamine (O-GlcNAc)...
  33. pmc dbOGAP - an integrated bioinformatics resource for protein O-GlcNAcylation
    Jinlian Wang
    Department of Oncology, Georgetown University Medical Center, NW, Washington, DC 20007, USA
    BMC Bioinformatics 12:91. 2011
    Protein O-GlcNAcylation (or O-GlcNAc-ylation) is an O-linked glycosylation involving the transfer of β-N-acetylglucosamine to the hydroxyl group of serine or threonine residues of proteins...
  34. pmc Critical role of O-Linked β-N-acetylglucosamine transferase in prostate cancer invasion, angiogenesis, and metastasis
    Thomas P Lynch
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102, USA
    J Biol Chem 287:11070-81. 2012
    ..the hexosamine biosynthetic pathway leads to increases in the post-translational addition of O-linked β-N-acetylglucosamine (O-GlcNAc) to various nuclear and cytosolic proteins...
  35. ncbi O-GlcNAc modification of FoxO1 increases its transcriptional activity: a role in the glucotoxicity phenomenon?
    Meishiue Kuo
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Biochimie 90:679-85. 2008
    ....
  36. pmc Modulation of transcription factor function by O-GlcNAc modification
    Sabire Ozcan
    Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA
    Biochim Biophys Acta 1799:353-64. 2010
    O-linked beta-N-acetylglucosamine (O-GlcNAc) modification of nuclear and cytoplasmic proteins is important for many cellular processes, and the number of proteins that contain this modification is steadily increasing...
  37. ncbi A novel human serum lectin with collagen- and fibrinogen-like domains that functions as an opsonin
    M Matsushita
    Department of Biochemistry, Fukushima Medical College, Japan
    J Biol Chem 271:2448-54. 1996
    ..Therefore, P35 represents a new type of GlcNAc-binding lectin with structural and functional similarities to collectins involved in innate immunity...
  38. ncbi Increased hexosamine biosynthesis and protein O-GlcNAc levels associated with myocardial protection against calcium paradox and ischemia
    Jia Liu
    Department of Cell Biology and Medicine, University of Alabama at Birmingham, 1530 3rd Avenue South, MCLM 690, Birmingham, AL 35294 0005, USA
    J Mol Cell Cardiol 40:303-12. 2006
    Increased hexosamine biosynthesis pathway (HBP) flux and elevated levels of protein O-linked-N-acetylglucosamine (O-GlcNAc) decrease calcium influx into isolated cardiomyocytes...
  39. ncbi In silico modulation of HMGN-1 binding to histones and gene expression by interplay of phosphorylation and O-GlcNAc modification
    Ishtiaq Ahmad
    Institute of Molecular Sciences and Bioinformatics, Lahore, Pakistan
    Protein Pept Lett 15:193-9. 2008
    ....
  40. ncbi Identification of O-linked N-acetylglucosamine proteins in rat skeletal muscle using two-dimensional gel electrophoresis and mass spectrometry
    Caroline Cieniewski-Bernard
    Unite Mixte de Recherche, Centre National de la Recherche Scientifique 8576, Glycobiologie Structurale et Fonctionnelle, IFR118, Villeneuve d Ascc, France
    Mol Cell Proteomics 3:577-85. 2004
    ..as a nutritional sensor, the level of UDP-GlcNAc that serves as a donor for the uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetyl-glucosaminyltransferase being regulated by the cellular fate of glucose...
  41. ncbi LysM, a widely distributed protein motif for binding to (peptido)glycans
    Girbe Buist
    Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    Mol Microbiol 68:838-47. 2008
    ..65 amino acid residues and binds to various types of peptidoglycan and chitin, most likely recognizing the N-acetylglucosamine moiety...
  42. pmc Inactivation of phosphorylated endothelial nitric oxide synthase (Ser-1177) by O-GlcNAc in diabetes-associated erectile dysfunction
    Biljana Musicki
    Department of Urology, The Johns Hopkins Hospital, 600 North Wolfe Street, Baltimore, MD 21287, USA
    Proc Natl Acad Sci U S A 102:11870-5. 2005
    ..In vivo penile erection paradigm supports the physiologic relevance of O-GlcNAc modification in vascular disorders associated with diabetes...
  43. pmc Protein O-GlcNAcylation: a new signaling paradigm for the cardiovascular system
    Boglarka Laczy
    Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294 0007, USA
    Am J Physiol Heart Circ Physiol 296:H13-28. 2009
    ..residues of nuclear and cytoplasmic proteins by the O-linked attachment of the monosaccharide beta-N-acetylglucosamine (O-GlcNAc) is a highly dynamic and ubiquitous protein modification...
  44. ncbi Mapping sites of O-GlcNAc modification using affinity tags for serine and threonine post-translational modifications
    Lance Wells
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21218, USA
    Mol Cell Proteomics 1:791-804. 2002
    ..O-Linked beta-N-acetylglucosamine (O-GlcNAc) is a dynamic nucleocytoplasmic modification more analogous to phosphorylation than to classical ..
  45. pmc Identification of an N-acetylglucosamine transporter that mediates hyphal induction in Candida albicans
    Francisco J Alvarez
    Graduate Program in Genetics, State University of New York, Stony Brook, NY 11794 5222, USA
    Mol Biol Cell 18:965-75. 2007
    The sugar N-acetylglucosamine (GlcNAc) plays an important role in nutrient sensing and cellular regulation in a wide range of organisms from bacteria to humans...
  46. pmc N-acetylglucosamine: production and applications
    Jeen Kuan Chen
    Department of Environment and Biotechnology, Refining and Manufacturing Research Institute, CPC Corporation, 217 Min Sheng S Rd, Chiayi, Taiwan
    Mar Drugs 8:2493-516. 2010
    N-Acetylglucosamine (GlcNAc) is a monosaccharide that usually polymerizes linearly through (1,4)-β-linkages. GlcNAc is the monomeric unit of the polymer chitin, the second most abundant carbohydrate after cellulose...
  47. ncbi Dynamic O-GlcNAc modification of nucleocytoplasmic proteins in response to stress. A survival response of mammalian cells
    Natasha E Zachara
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 279:30133-42. 2004
    ..A wide variety of stress stimuli rapidly increase nucleocytoplasmic protein modification by O-linked beta-N-acetylglucosamine (O-GlcNAc), an essential post-translational modification of Ser and Thr residues of metazoans...
  48. pmc The O-GlcNAc transferase gene resides on the X chromosome and is essential for embryonic stem cell viability and mouse ontogeny
    R Shafi
    The Howard Hughes Medical Institute, Glycobiology Research and Training Center, Department of Cellular and Molecular Medicine, 9500 Gilman Drive 0625, University of California San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 97:5735-9. 2000
    ..Intracellular glycosylation by the addition of N-acetylglucosamine (GlcNAc) to serine and threonine is catalyzed by the O-GlcNAc transferase (OGT)...
  49. pmc O-GlcNAc cycling: implications for neurodegenerative disorders
    Brooke D Lazarus
    Laboratory of Cell Biology and Biochemistry, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Int J Biochem Cell Biol 41:2134-46. 2009
    The dynamic post-translational modification of proteins by O-linked N-acetylglucosamine (O-GlcNAc), termed O-GlcNAcylation, is an important mechanism for modulating cellular signaling pathways...
  50. pmc Hyperglycemia-induced mitochondrial superoxide overproduction activates the hexosamine pathway and induces plasminogen activator inhibitor-1 expression by increasing Sp1 glycosylation
    X L Du
    Albert Einstein College of Medicine, Diabetes Research Center, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 97:12222-6. 2000
    ..Thus, hyperglycemia-induced mitochondrial superoxide overproduction increases hexosamine synthesis and O-glycosylation of Sp1, which activates expression of genes that contribute to the pathogenesis of diabetic complications...
  51. pmc In vivo imaging of Caenorhabditis elegans glycans
    Scott T Laughlin
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    ACS Chem Biol 4:1068-72. 2009
    ..elegans. We treated worms with azidosugar variants of N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc), and N-acetylmannosamine (ManNAc), resulting in the metabolic ..
  52. pmc Increased enzymatic O-GlcNAcylation of mitochondrial proteins impairs mitochondrial function in cardiac myocytes exposed to high glucose
    Yong Hu
    Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 284:547-55. 2009
    Increased nuclear protein O-linked beta-N-acetylglucosamine glycosylation (O-GlcNAcylation) mediated by high glucose treatment or the hyperglycemia of diabetes mellitus contributes to cardiac myocyte dysfunction...
  53. pmc Protein modification by O-linked GlcNAc reduces angiogenesis by inhibiting Akt activity in endothelial cells
    Bai Luo
    Division of Endocrinology, University of Utah, Salt Lake City, UT 84132, USA
    Arterioscler Thromb Vasc Biol 28:651-7. 2008
    ..O-linked N-acetylglucosamine (O-GlcNAc) modification on protein is the major mechanism mediating the actions of the HBP...
  54. ncbi Peptidoglycan N-acetylglucosamine deacetylation decreases autolysis in Lactococcus lactis
    Mickael Meyrand
    INRA, Unité de Biochimie Bactérienne, UR477, 78350 Jouy en Josas, France
    Microbiology 153:3275-85. 2007
    The gene xynD (renamed pgdA) of Lactococcus lactis IL1403 was shown to encode a peptidoglycan N-acetylglucosamine deacetylase. Inactivation of pgdA in L...
  55. pmc Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins
    Peter M Clark
    Division of Chemistry and Chemical Engineering and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    J Am Chem Soc 130:11576-7. 2008
    ..As such, this strategy enables studies of O-GlcNAc glycosylation that were previously inaccessible and provides a new tool for uncovering the physiological functions of O-GlcNAc...
  56. doi Targeting of macrophage galactose-type C-type lectin (MGL) induces DC signaling and activation
    Chiara Napoletano
    Department of Experimental Medicine, Sapienza University, Rome, Italy
    Eur J Immunol 42:936-45. 2012
    ..These results demonstrate that the targeting of MGL receptor on human DCs has an adjuvant effect and that this strategy can be used to design novel anticancer vaccines...
  57. ncbi N-Acetylglucosamine 6-O-sulfotransferase-1 is required for brain keratan sulfate biosynthesis and glial scar formation after brain injury
    Haoqian Zhang
    Department of Biochemistry, Institute for Advanced Research, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Glycobiology 16:702-10. 2006
    ..composed of repeating disaccharide units with sulfate residues at the C6 positions of galactose and N-acetylglucosamine (GlcNAc)...
  58. ncbi A new strategy for identification of N-glycosylated proteins and unambiguous assignment of their glycosylation sites using HILIC enrichment and partial deglycosylation
    Per Hägglund
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    J Proteome Res 3:556-66. 2004
    ..2.1.96). After hydrolysis with these enzymes, a single N-acetylglucosamine (GlcNAc) residue remains linked to the asparagine residue...
  59. pmc O-linked N-acetylglucosamine modification on CCAAT enhancer-binding protein beta: role during adipocyte differentiation
    Xi Li
    Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University Shanghai Medical College, Shanghai 200032, China
    J Biol Chem 284:19248-54. 2009
    ..Mutation of both Ser(180) and Ser(181) to Ala significantly increase the transcriptional activity of C/EBPbeta. These data suggest that GlcNAcylation regulates both the phosphorylation and DNA binding activity of C/EBPbeta...
  60. ncbi Phosphoenolpyruvate phosphotransferase system and N-acetylglucosamine metabolism in Bacillus sphaericus
    Alejandro F Alice
    Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
    Microbiology 149:1687-98. 2003
    ..has some unusual features, as they are co-transcribed with genes encoding metabolic enzymes related to N-acetylglucosamine (GlcNAc) catabolism (nagA, nagB and an undetermined orf2)...
  61. pmc N-acetylglucosamine (GlcNAc) induction of hyphal morphogenesis and transcriptional responses in Candida albicans are not dependent on its metabolism
    Shamoon Naseem
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794 5222, USA
    J Biol Chem 286:28671-80. 2011
    N-acetylglucosamine (GlcNAc) stimulates important signaling pathways in a wide range of organisms...
  62. ncbi Different regions of Mlc and NagC, homologous transcriptional repressors controlling expression of the glucose and N-acetylglucosamine phosphotransferase systems in Escherichia coli, are required for inducer signal recognition
    Carole Pennetier
    Institut de Biologie Physico Chimique UPR9073 CNRS, 13 rue Pierre et Marie Curie, 75005 Paris, France
    Mol Microbiol 67:364-77. 2008
    ....
  63. pmc Dysregulation of insulin signaling, glucose transporters, O-GlcNAcylation, and phosphorylation of tau and neurofilaments in the brain: Implication for Alzheimer's disease
    Yanqiu Deng
    Department of Neurochemistry, New York State Institute for Basic Research in Developmental Disabilities, Staten Island, New York, NY 10314, USA
    Am J Pathol 175:2089-98. 2009
    ....
  64. pmc O-GLcNAc post-translational modifications regulate the entry of neurons into an axon branching program
    Herb Francisco
    Department of Neurobiology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA
    Dev Neurobiol 69:162-73. 2009
    ..cytosolic and nuclear proteins are post-translationally modified by the reversible addition of O-linked N-acetylglucosamine (O-GlcNAc) on serines and threonines...
  65. pmc O-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation
    Takahiro Isono
    Central Research Laboratory, Shiga University of Medical Science, Otsu, Shiga, Japan
    PLoS ONE 6:e18959. 2011
    Modification of serine and threonine residues in proteins by O-linked β-N-acetylglucosamine (O-GlcNAc) glycosylation is a feature of many cellular responses to the nutritional state and to stress...
  66. ncbi Effect of the Met344His mutation on the conformational dynamics of bovine beta-1,4-galactosyltransferase: crystal structure of the Met344His mutant in complex with chitobiose
    Boopathy Ramakrishnan
    Structural Glycobiology Section, Laboratory of Experimental and Computational Biology, SAIC Frederick, Inc, Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702 1201, USA
    Biochemistry 43:12513-22. 2004
    ..beta4Gal-T1) in the presence of manganese ion transfers galactose from UDP-galactose (UDP-Gal) to N-acetylglucosamine (GlcNAc) that is either free or linked to an oligosaccharide...
  67. doi O-linked-N-acetylglucosamine on extracellular protein domains mediates epithelial cell-matrix interactions
    Yuta Sakaidani
    Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa Ku, Nagoya 466 0065, Japan
    Nat Commun 2:583. 2011
    The O-linked-N-acetylglucosamine (O-GlcNAc) modification of cytoplasmic and nuclear proteins regulates basic cellular functions and is involved in the aetiology of diabetes and neurodegeneration...
  68. pmc Tandem mass spectrometry identifies many mouse brain O-GlcNAcylated proteins including EGF domain-specific O-GlcNAc transferase targets
    Joshua F Alfaro
    Pacific Northwest National Laboratory, Richland, WA 99352, USA
    Proc Natl Acad Sci U S A 109:7280-5. 2012
    O-linked N-acetylglucosamine (O-GlcNAc) is a reversible posttranslational modification of Ser and Thr residues on cytosolic and nuclear proteins of higher eukaryotes catalyzed by O-GlcNAc transferase (OGT)...
  69. pmc Insights into O-linked N-acetylglucosamine ([0-9]O-GlcNAc) processing and dynamics through kinetic analysis of O-GlcNAc transferase and O-GlcNAcase activity on protein substrates
    David L Shen
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada
    J Biol Chem 287:15395-408. 2012
    Cellular O-linked N-acetylglucosamine (O-GlcNAc) levels are modulated by two enzymes: uridine diphosphate-N-acetyl-D-glucosamine:polypeptidyltransferase (OGT) and O-GlcNAcase (OGA)...
  70. ncbi Modification of p53 with O-linked N-acetylglucosamine regulates p53 activity and stability
    Won Ho Yang
    Department of Biology, Yonsei University, 134 Shinchon dong, Seodaemun gu, Seoul 120 749, Korea
    Nat Cell Biol 8:1074-83. 2006
    Post-translational addition of O-linked N-acetylglucosamine (O-GlcNAc) to p53 is known to occur, but the site of O-GlcNAcylation and its effects on p53 are not understood...
  71. pmc Modification of histones by sugar β-N-acetylglucosamine (GlcNAc) occurs on multiple residues, including histone H3 serine 10, and is cell cycle-regulated
    Suisheng Zhang
    Genome Stability Laboratory, Center for Chromosome Biology, School of Natural Sciences, National University of Ireland Galway, University Road, Galway, Ireland
    J Biol Chem 286:37483-95. 2011
    The monosaccharide, β-N-acetylglucosamine (GlcNAc), can be added to the hydroxyl group of either serines or threonines to generate an O-linked β-N-acetylglucosamine (O-GlcNAc) residue (Love, D. C., and Hanover, J. A. (2005) Sci...
  72. pmc Binding properties of the N-acetylglucosamine and high-mannose N-glycan PP2-A1 phloem lectin in Arabidopsis
    Julie Beneteau
    UR1268 Unité de Recherches Biopolymères Interactions Assemblages, INRA, F 44300 Nantes, France
    Plant Physiol 153:1345-61. 2010
    ..Using a recombinant protein produced in Escherichia coli, we demonstrated binding to N-acetylglucosamine oligomers...
  73. pmc Regulon of the N-acetylglucosamine utilization regulator NagR in Bacillus subtilis
    Ralph Bertram
    Lehrbereich Mikrobielle Genetik, Eberhard Karls Universitat Tubingen, Waldhäuser Strasse 70 8, 72076 Tubingen, Germany
    J Bacteriol 193:3525-36. 2011
    N-Acetylglucosamine (GlcNAc) is the most abundant carbon-nitrogen biocompound on earth and has been shown to be an important source of nutrients for both catabolic and anabolic purposes in Bacillus species...
  74. pmc Chemical structure of Trichomonas vaginalis surface lipoglycan: a role for short galactose (β1-4/3) N-acetylglucosamine repeats in host cell interaction
    Christopher M Ryan
    Department of Microbiology, Immunology, and Molecular Genetics, UCLA, Los Angeles, California 90095 1489, USA
    J Biol Chem 286:40494-508. 2011
    ..analyzed the structure of this glycoconjugate, previously described to contain the sugars rhamnose (Rha), N-acetylglucosamine (GlcNAc), galactose (Gal), xylose (Xyl), N-acetylgalactosamine (GalNAc), and glucose (Glc), using gas ..
  75. ncbi Increased N-acetyl-beta-glucosaminidase activity in primary breast carcinomas corresponds to a decrease in N-acetylglucosamine containing proteins
    C Slawson
    Department of Chemistry and Institute for Biomolecular Science, University of South Florida, 4202 Fowler Avenue, Tampa, FL 33620, USA
    Biochim Biophys Acta 1537:147-57. 2001
    N-acetylglucosamine (O-GlcNAc) modification on serine or threonine residues of cytoplasmic and nuclear proteins has become a more recognized intracellular covalent modification...
  76. pmc Identification of the major site of O-linked beta-N-acetylglucosamine modification in the C terminus of insulin receptor substrate-1
    Lauren E Ball
    Department of Endocrinology, Diabetes, and Medical Genetics, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Mol Cell Proteomics 5:313-23. 2006
    ..Ser/Thr residues can also be reversibly glycosylated by O-linked beta-N-acetylglucosamine (O-GlcNAc) monosaccharide, a dynamic posttranslational modification that offers an alternative means of ..
  77. doi O-linked β-N-acetylglucosamine supports p38 MAPK activation by high glucose in glomerular mesangial cells
    Howard Goldberg
    Department of Medicine, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    Am J Physiol Endocrinol Metab 301:E713-26. 2011
    ..Thus, O-GlcNAcylation is coupled to profibrotic p38 MAPK signaling by high glucose in part through Akt and possibly through ROS...
  78. ncbi Cloning and characterization of the N-acetylglucosamine operon of Escherichia coli
    K G Peri
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada
    Biochem Cell Biol 68:123-37. 1990
    Three enzymes are required for N-acetylglucosamine (NAG) utilization in Escherichia coli: enzyme IInag (gene nagE), N-acetylglucosamine-6-phosphate deacetylase (gene nagA), and glucosamine-6-phosphate isomerase (gene nagB)...
  79. pmc Inhibition of O-GlcNAcase in perfused rat hearts by NAG-thiazolines at the time of reperfusion is cardioprotective in an O-GlcNAc-dependent manner
    Boglarka Laczy
    Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294 0007, USA
    Am J Physiol Heart Circ Physiol 299:H1715-27. 2010
    Acute increases in O-linked β-N-acetylglucosamine (O-GlcNAc) levels of cardiac proteins exert protective effects against ischemia-reperfusion (I/R) injury...
  80. pmc Augmented O-GlcNAc signaling attenuates oxidative stress and calcium overload in cardiomyocytes
    Gladys A Ngoh
    Department of Physiology and Biophysics, Diabetes and Obesity Center, Institute of Molecular Cardiology, University of Louisville, Louisville, KY 40202, USA
    Amino Acids 40:895-911. 2011
    O-linked β-N-acetylglucosamine (O-GlcNAc) is an inducible, dynamically cycling and reversible post-translational modification of Ser/Thr residues of nucleocytoplasmic and mitochondrial proteins...
  81. pmc Identification of a dedicated recycling pathway for anhydro-N-acetylmuramic acid and N-acetylglucosamine derived from Escherichia coli cell wall murein
    J T Park
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    J Bacteriol 183:3842-7. 2001
    ..It is demonstrated here that E. coli recycles the N-acetylglucosamine present in cell wall murein degradation products for de novo murein and lipopolysaccharide synthesis...
  82. doi Evidence of O-linked N-acetylglucosamine in diabetic nephropathy
    Peter Degrell
    2nd Department of Medicine and Nephrological Center, University of Pecs, Hungary
    Life Sci 84:389-93. 2009
    There is increasing evidence that O-linked N-acetylglucosamine (O-GlcNAc) plays an important role in cell signaling pathways...
  83. pmc O-linked GlcNAc modification of cardiac myofilament proteins: a novel regulator of myocardial contractile function
    Genaro A Ramirez-Correa
    Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Circ Res 103:1354-8. 2008
    ..This study provides the first identification of O-GlcNAcylation sites in cardiac myofilament proteins and demonstrates their potential role in regulating myocardial contractile function...
  84. pmc Regulation of insulin receptor substrate 1 (IRS-1)/AKT kinase-mediated insulin signaling by O-Linked beta-N-acetylglucosamine in 3T3-L1 adipocytes
    Stephen A Whelan
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 285:5204-11. 2010
    Increased O-linked beta-N-acetylglucosamine (O-GlcNAc) is associated with insulin resistance in muscle and adipocytes. Upon insulin treatment of insulin-responsive adipocytes, O-GlcNAcylation of several proteins is increased...
  85. doi Cardioprotection of salidroside from ischemia/reperfusion injury by increasing N-acetylglucosamine linkage to cellular proteins
    Tiejun Wu
    Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military Medical University, Xi an, China
    Eur J Pharmacol 613:93-9. 2009
    The modification of proteins with O-linked N-acetylglucosamine (O-GlcNAc) is increasingly recognized as an important posttranslational modification that modulates cellular function...
  86. pmc Up-regulation of O-GlcNAc transferase with glucose deprivation in HepG2 cells is mediated by decreased hexosamine pathway flux
    Rodrick P Taylor
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    J Biol Chem 284:3425-32. 2009
    O-Linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of proteins that functions as a nutrient sensing mechanism...
  87. pmc O-GlcNAc signaling attenuates ER stress-induced cardiomyocyte death
    Gladys A Ngoh
    Institute of Molecular Cardiology, University of Louisville, Louisville, Kentucky, USA
    Am J Physiol Heart Circ Physiol 297:H1711-9. 2009
    We previously demonstrated that the O-linked beta-N-acetylglucosamine (O-GlcNAc) posttranslational modification confers cardioprotection at least partially through mitochondrial-dependent mechanisms, but it remained unclear if O-GlcNAc ..
  88. pmc O-GlcNAc protein modification in plants: Evolution and function
    Neil E Olszewski
    Department of Plant Biology, Microbial and Plant Genomics Institute, 250 Biological Sciences Center, 1445 Gortner Ave, St Paul, MN 55108, USA
    Biochim Biophys Acta 1800:49-56. 2010
    The role in plants of posttranslational modification of proteins with O-linked N-acetylglucosamine and the evolution and function of O-GlcNAc transferases responsible for this modification are reviewed...
  89. ncbi O-glycosylation of FoxO1 increases its transcriptional activity towards the glucose 6-phosphatase gene
    Meishiue Kuo
    Institut Cochin, Universite Paris Descartes, 22 rue Mechain, CNRS UMR 8104, Paris, France
    FEBS Lett 582:829-34. 2008
    ..Since glucose-6-phosphatase plays a key role in hepatic glucose production, our observation may be of importance with regard to glucotoxicity associated with chronic hyperglycaemia in diabetes...
  90. ncbi GlcNAcylation plays an essential role in breast cancer metastasis
    Yuchao Gu
    Department of Molecular Biology, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China
    Cancer Res 70:6344-51. 2010
    ..These results also suggest that GlcNAcylation might be a potential target for the diagnosis and therapy of breast cancer...
  91. pmc Activation of the hexosamine biosynthesis pathway and protein O-GlcNAcylation modulate hypertrophic and cell signaling pathways in cardiomyocytes from diabetic mice
    Susan A Marsh
    Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294 0007, USA
    Amino Acids 40:819-28. 2011
    ..glucose metabolism via the hexosamine biosynthesis pathway (HBP) and associated increase in O-linked-β-N-acetylglucosamine (O-GlcNAc) levels on proteins contributed to the adverse effects of diabetes on the heart...
  92. ncbi O-linked N-acetylglucosamine is present on the extracellular domain of notch receptors
    Aiko Matsuura
    Department of Applied Molecular Biosciences, Nagoya University Graduate School of Bioagricultural Sciences, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    J Biol Chem 283:35486-95. 2008
    ..Thus, the finding of O-GlcNAc modification in extracellular environments predicts a distinct glycosylation process that might be associated with a novel regulatory mechanism for Notch receptor activity...
  93. ncbi Probing the dynamics of O-GlcNAc glycosylation in the brain using quantitative proteomics
    Nelly Khidekel
    Division of Chemistry and Chemical Engineering and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    Nat Chem Biol 3:339-48. 2007
    ..Together, our studies suggest that O-GlcNAc glycosylation occurs reversibly in neurons and, akin to phosphorylation, may have important roles in mediating the communication between neurons...
  94. pmc Aging leads to increased levels of protein O-linked N-acetylglucosamine in heart, aorta, brain and skeletal muscle in Brown-Norway rats
    Norbert Fülöp
    Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294 0005, USA
    Biogerontology 9:139-51. 2008
    ....
  95. ncbi Aberrant O-GlcNAcylation characterizes chronic lymphocytic leukemia
    Y Shi
    Division of Molecular and Cellular Biology, Research Institute, Sunnybrook Health Sciences Center, Toronto, Ontario, Canada
    Leukemia 24:1588-98. 2010
    O-linked N-Acetylglucosamine (O-GlcNAc) post-translational modifications originate from the activity of the hexosamine pathway, and are known to affect intracellular signaling processes...
  96. pmc Increased O-GlcNAc levels during reperfusion lead to improved functional recovery and reduced calpain proteolysis
    Jia Liu
    Department of Cell Biology, University of Alabama at Birmingham, AL 35294 0005, USA
    Am J Physiol Heart Circ Physiol 293:H1391-9. 2007
    ..in part, via enhanced flux through the hexosamine biosynthesis pathway and subsequently elevated O-linked N-acetylglucosamine (O-GlcNAc) protein levels...
  97. ncbi Glucosamine administration improves survival rate after severe hemorrhagic shock combined with trauma in rats
    Laszlo G Nöt
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294 0005, USA
    Shock 28:345-52. 2007
    ..with resuscitation in rats, which was associated with the increased levels of protein O-linked-N-acetylglucosamine (O-GlcNAc)...
  98. pmc Poly-N-acetylglucosamine mediates biofilm formation and detergent resistance in Aggregatibacter actinomycetemcomitans
    Era A Izano
    Department of Oral Biology, New Jersey Dental School, 185 S Orange Avenue, Newark, NJ 07103, USA
    Microb Pathog 44:52-60. 2008
    ..Our findings suggest that PGA, extracellular DNA and proteinaceous adhesins all contribute to the structural integrity of the A. actinomycetemcomitans biofilm matrix...
  99. ncbi Biosynthesis of N-acetylneuraminic acid in cells lacking UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
    S Hinderlich
    , , Berlin-Dahlem, Germany
    Biol Chem 382:291-7. 2001
    ..carbohydrate moiety in biological recognition systems, are performed by the bifunctional enzyme UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase...
  100. doi Mapping of O-linked beta-N-acetylglucosamine modification sites in key contractile proteins of rat skeletal muscle
    Julie Hedou
    Laboratoire de Plasticite Neuromusculaire, Unité de Neurosciences et Physiologies Adaptatives, UPRES EA 4052, IFR 147, Université des Sciences et Technologies de Lille 1, Villeneuve d Ascq, France
    Proteomics 9:2139-48. 2009
    O-linked beta-N-acetylglucosamine (O-GlcNAc) is a widespread modification of serine/threonine residues of nucleocytoplasmic proteins. Recently, several key contractile proteins in rat skeletal muscle (i.e...
  101. ncbi alpha-Lactalbumin (LA) stimulates milk beta-1,4-galactosyltransferase I (beta 4Gal-T1) to transfer glucose from UDP-glucose to N-acetylglucosamine. Crystal structure of beta 4Gal-T1 x LA complex with UDP-Glc
    B Ramakrishnan
    Structural Glycobiology Section, Laboratory of Experimental and Computational Biology, Center for Cancer Research, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
    J Biol Chem 276:37665-71. 2001
    beta-1,4-Galactosyltransferase 1 (Gal-T1) transfers galactose (Gal) from UDP-Gal to N-acetylglucosamine (GlcNAc), which constitutes its normal galactosyltransferase (Gal-T) activity...

Research Grants76

  1. Environmental Chitin Exposure in the Pathogenesis of Asthma
    Rene M Roy; Fiscal Year: 2010
    ..Chitin, which is the second most abundant polymer on our planet, is a linear polymer of N- acetylglucosamine. In recent work, exposure of mice to chitin particles induced the accumulation of immune cells associated ..
  2. PROTEIN-CARBOHYDRATE INTERACTION
    Irwin Goldstein; Fiscal Year: 1990
    ..The lima bean lectin gene will be cloned and translated; site-specific mutagenesis should allow identification of amino acid residues involved in carbohydrate binding...
  3. Photocleavable Bead Technology for Glycomics
    Mark Lim; Fiscal Year: 2012
    ..builds on the recently reported use of AmberGen's photocleavable linkers to identify O-linked beta- N-acetylglucosamine (O-GlcNAc) protein modifications in cells, tissues and other biospecimens...
  4. Collagen I, Hyaluronan, and Aging
    May J Reed; Fiscal Year: 2010
    ..Notably, HA - a simple polymer of the disaccharide D-glucuronic acid/D-N-acetylglucosamine - has differential effects on angiogenesis that are related to its chain length...
  5. HTS assay development for inhibitors of sickle cell
    PAUL FRENETTE; Fiscal Year: 2006
    ..catalyze the formation of an alpha anomeric glycosidic bond between carbon 1 of the fucose and carbon 3 of N-acetylglucosamine. We have developed an ELISA-based assay to evaluate rapidly the FucT activity in cell lysates...
  6. HTS assay development for inhibitors of sickle cell
    PAUL FRENETTE; Fiscal Year: 2005
    ..catalyze the formation of an alpha anomeric glycosidic bond between carbon 1 of the fucose and carbon 3 of N-acetylglucosamine. We have developed an ELISA-based assay to evaluate rapidly the FucT activity in cell lysates...
  7. COMPLEMENT ACTIVATION BY MANNOSE-BINDING PROTEIN
    Keith Joiner; Fiscal Year: 1993
    ..with rough mutants and smooth wild type strains, by attempting to block MBP activity with mannose or N-acetylglucosamine or with MAb directed against each LPS chemotype, and by examining LPS lengths optimum for interactions with ..
  8. COMPLEMENT ACTIVATION BY MANNOSE-BINDING PROTEIN
    JO SCHWEINLE; Fiscal Year: 1990
    ..with rough mutants and smooth wild type strains, by attempting to block MBP activity with mannose or N-acetylglucosamine or with MAb directed against each LPS chemotype, and by examining LPS lengths optimum for interactions with ..
  9. CHITIN BIOSYNTHESIS AND THE MOSQUITO PERITROPHIC MATRIX
    Bruce Christensen; Fiscal Year: 2004
    ..N-acetyltransferase (GNAT), phosphoacetyl glucosamine mutase (AGM), and uridine diphosphate-N-acetylglucosamine pyrophophrylase (UAP) are also involved in this pathway but have never been cloned or characterized in any ..
  10. CHITIN BIOSYNTHESIS AND THE MOSQUITO PERITROPHIC MATRIX
    Bruce Christensen; Fiscal Year: 2005
    ..N-acetyltransferase (GNAT), phosphoacetyl glucosamine mutase (AGM), and uridine diphosphate-N-acetylglucosamine pyrophophrylase (UAP) are also involved in this pathway but have never been cloned or characterized in any ..
  11. Regulation of structure and function of protein by glycosylation
    Zhiwen Zhang; Fiscal Year: 2009
    ..We are specifically interested in O-N-acetylglucosamine (O-GlcNAc) modification, where the GlcNAc moiety is attached by an ether linkage to a serine or threonine ..
  12. Regulation of structure and function of protein by glycosylation
    Zhiwen Jonathan Zhang; Fiscal Year: 2010
    ..We are specifically interested in O-N-acetylglucosamine (O-GlcNAc) modification, where the GlcNAc moiety is attached by an ether linkage to a serine or threonine ..
  13. CELL RECEPTORS IN COAGULATION AND ATHEROGENESIS
    SALVATORE PIZZO; Fiscal Year: 1980
    ..be evaluated: the reticuloendothelial system; hepatocyte and macrophage receptors for exposed galactose, N-acetylglucosamine, mannose and fucose; a receptor for clustered positive charges; a unique protease-inhibitor receptor(s)...
  14. TRANSPORT AND PROCESSING OF THE ADENOVIRUS FIBER PROTEIN
    JEFFREY ENGLER; Fiscal Year: 1991
    ..Fiber from adenovirus serotypes 2 and 5 contains an unusual O-linked N-acetylglucosamine (O-GlcNAc), a saccharide modification found on many cytoplasmic and nuclear proteins (such as the proteins ..
  15. Structural and Biochemical Studies of LpxC Inhibition
    Pei Zhou; Fiscal Year: 2010
    ..The committed step of lipid A biosynthesis is catalyzed by UDP-3-O-(acyl)-N-acetylglucosamine deacetylase (LpxC)...
  16. TWO REGULATORY ENZYMES OF DOLICHOL-LINKED GLYCOSYLATION
    SHARON KRAG; Fiscal Year: 1990
    ..Both enzymes, UDP-N-acetylglucosamine:dolichyl phosphate N- acetylglucosamine-1-phosphate transferase and mannosylphosphoryldolichol synthase, are ..
  17. Structural and Biochemical Studies of LpxC Inhibition
    Pei Zhou; Fiscal Year: 2009
    ..The committed step of lipid A biosynthesis is catalyzed by UDP-3-O-(acyl)-N-acetylglucosamine deacetylase (LpxC)...
  18. Structural and Biochemical Studies of LpxC Inhibition
    Pei Zhou; Fiscal Year: 2009
    ..The committed step of lipid A biosynthesis is catalyzed by UDP-3-O-(acyl)-N- acetylglucosamine deacetylase (LpxC)...
  19. CHITIN BIOSYNTHESIS AND THE MOSQUITO PERITROPHIC MATRIX
    Bruce Christensen; Fiscal Year: 2003
    ..N-acetyltransferase (GNAT), phosphoacetyl glucosamine mutase (AGM), and uridine diphosphate-N-acetylglucosamine pyrophophrylase (UAP) are also involved in this pathway but have never been cloned or characterized in any ..
  20. CHITIN BIOSYNTHESIS AND THE MOSQUITO PERITROPHIC MATRIX
    Bruce Christensen; Fiscal Year: 2007
    ..N-acetyltransferase (GNAT), phosphoacetyl glucosamine mutase (AGM), and uridine diphosphate-N-acetylglucosamine pyrophophrylase (UAP) are also involved in this pathway but have never been cloned or characterized in any ..
  21. CHITIN BIOSYNTHESIS AND THE MOSQUITO PERITROPHIC MATRIX
    Bruce Christensen; Fiscal Year: 2006
    ..N-acetyltransferase (GNAT), phosphoacetyl glucosamine mutase (AGM), and uridine diphosphate-N-acetylglucosamine pyrophophrylase (UAP) are also involved in this pathway but have never been cloned or characterized in any ..
  22. 'Immunity of Schistosome Glycoconjugates'
    Richard Cummings; Fiscal Year: 2001
    ..five major antigenic glycans in bi- and trisaccharide sequences containing fucose, galactose, xylose, N-acetylglucosamine and N-acetylgalactosamine...
  23. Vibrio Cholerae Colonization of Chitin Surfaces
    Gary Schoolnik; Fiscal Year: 2005
    ..Chitin is an insoluble polymer of N-acetylglucosamine; through the secretion of chitinases, V...
  24. Vibrio Cholerae Colonization of Chitin Surfaces
    Gary Schoolnik; Fiscal Year: 2004
    ..Chitin is an insoluble polymer of N-acetylglucosamine; through the secretion of chitinases, V...
  25. Vibrio Cholerae Colonization of Chitin Surfaces
    Gary Schoolnik; Fiscal Year: 2006
    ..Chitin is an insoluble polymer of N-acetylglucosamine; through the secretion of chitinases, V...
  26. Vibrio Cholerae Colonization of Chitin Surfaces
    Gary Schoolnik; Fiscal Year: 2007
    ..Chitin is an insoluble polymer of N-acetylglucosamine; through the secretion of chitinases, V...
  27. Vibrio Cholerae Colonization of Chitin Surfaces
    Gary Schoolnik; Fiscal Year: 2003
    ..Chitin is an insoluble polymer of N-acetylglucosamine; through the secretion of chitinases, V...
  28. MEMBRANE TOPOLOGY AND BIOSYNTHESIS OF GLYCOSAMINOGLYCANS
    CARLOS HIRSCHBERG; Fiscal Year: 1999
    ..Determine the recognition domains for nucleotides, sugars and sulfate of the MDCK transporters for UDP- N-acetylglucosamine and PAPS by using a combination of photolabelling, cross-linking and mutagenesis...
  29. STRUCTURAL ANALYSIS OF GLYCOCONJUGATE METABOLISM
    Patrick Van Roey; Fiscal Year: 1999
    ..Endoglycosidases are glycohydrolases that cleave the glycosidic link between the two N-acetylglucosamine residues in the core of the oligosaccharide chain...
  30. STRUCTURAL ANALYSIS OF GLYCOCONJUGATE METABOLISM
    Patrick Van Roey; Fiscal Year: 2000
    ..Endoglycosidases are glycohydrolases that cleave the glycosidic link between the two N-acetylglucosamine residues in the core of the oligosaccharide chain...
  31. MUTANT ALTERED IN GLYCOSYLATION OF MEMBRANE PROTEINS
    SHARON KRAG; Fiscal Year: 1993
    ..Second, an oligosaccharide is synthesized attached to dolichyl phosphate by stepwise additions of N-acetylglucosamine, mannose, and glucose from sugar nucleotides and monoglycosylated, phosphorylated dolichols...
  32. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 1990
    ..oligosaccharides begins with the transfer of a preformed oligosaccharide containing glucose, mannose and N-acetylglucosamine from dolichyl pyrophosphate to asparagine residues in protein...
  33. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 1991
    ..oligosaccharides begins with the transfer of a preformed oligosaccharide containing glucose, mannose and N-acetylglucosamine from dolichyl pyrophosphate to asparagine residues in protein...
  34. GLUCOSAMINE IN CARTILAGE HOMEOSTASIS AND DEGRADATION
    ALEXANDER SHIKHMAN; Fiscal Year: 2000
    ..Recently, we discovered that glucosamine (GlcN) and its derivative, N-acetylglucosamine (GlcNAc), express a broad range of anti-inflammatory activities in cultured human articular chondrocytes ..
  35. GLUCOSAMINE IN CARTILAGE HOMEOSTASIS AND DEGRADATION
    ALEXANDER SHIKHMAN; Fiscal Year: 2003
    ..Recently, we discovered that glucosamine (GlcN) and its derivative, N-acetylglucosamine (GlcNAc), express a broad range of anti-inflammatory activities in cultured human articular chondrocytes ..
  36. GLUCOSAMINE IN CARTILAGE HOMEOSTASIS AND DEGRADATION
    ALEXANDER SHIKHMAN; Fiscal Year: 2001
    ..Recently, we discovered that glucosamine (GlcN) and its derivative, N-acetylglucosamine (GlcNAc), express a broad range of anti-inflammatory activities in cultured human articular chondrocytes ..
  37. REGULATION OF GLCNAC-PI DEACETYLATION
    Victoria Stevens; Fiscal Year: 1999
    ..this proposal, we will study the regulation of the second step in GPI biosynthesis, the deacetylation of N-acetylglucosamine-phosphatidylinositol (GlcNAc-PI) to form glucosamine-phosphatidylinositol (GlcN-PI)...
  38. GLUCOSAMINE IN CARTILAGE HOMEOSTASIS AND DEGRADATION
    ALEXANDER SHIKHMAN; Fiscal Year: 2002
    ..Recently, we discovered that glucosamine (GlcN) and its derivative, N-acetylglucosamine (GlcNAc), express a broad range of anti-inflammatory activities in cultured human articular chondrocytes ..
  39. GLUCOSAMINE IN CARTILAGE HOMEOSTASIS AND DEGRADATION
    ALEXANDER SHIKHMAN; Fiscal Year: 2004
    ..Recently, we discovered that glucosamine (GlcN) and its derivative, N-acetylglucosamine (GlcNAc), express a broad range of anti-inflammatory activities in cultured human articular chondrocytes ..
  40. 'Immunity of Schistosome Glycoconjugates'
    Richard Cummings; Fiscal Year: 2002
    ..These antigenic glycans occur in di- and trisaccharide sequences containing fucose, galactose, xylose, N-acetylglucosamine and N-acetylgalactosamine...
  41. Regulation of IGF-1 and Insulin Signaling by O-GlcNAc Glycosylation
    Lauren E Ball; Fiscal Year: 2010
    ..IRS-1 and IRS-2 are also glycosylated at Ser/Thr residues by N-acetylglucosamine (O-GlcNAc)...
  42. 'Immunity of Schistosome Glycoconjugates'
    Richard Cummings; Fiscal Year: 2001
    ..These antigenic glycans occur in di- and trisaccharide sequences containing fucose, galactose, xylose, N-acetylglucosamine and N-acetylgalactosamine...
  43. 'Immunity of Schistosome Glycoconjugates'
    Richard Cummings; Fiscal Year: 2003
    ..These antigenic glycans occur in di- and trisaccharide sequences containing fucose, galactose, xylose, N-acetylglucosamine and N-acetylgalactosamine...
  44. INNOVATIVE INTRAVESICAL TREATMENT APPROACH FOR IC
    Veer Bhavanandan; Fiscal Year: 1999
    ..Preliminary studies have demonstrated the presence of galactose- and N-acetylglucosamine-binding lectins in both rabbit and human bladders...
  45. INNOVATIVE INTRAVESICAL TREATMENT APPROACH FOR IC
    Veer Bhavanandan; Fiscal Year: 2001
    ..Preliminary studies have demonstrated the presence of galactose- and N-acetylglucosamine-binding lectins in both rabbit and human bladders...
  46. INNOVATIVE INTRAVESICAL TREATMENT APPROACH FOR IC
    Veer Bhavanandan; Fiscal Year: 2000
    ..Preliminary studies have demonstrated the presence of galactose- and N-acetylglucosamine-binding lectins in both rabbit and human bladders...
  47. STRUCTURES OF NEURECTODERM TUMOR GLYCOPROTEINS
    MARY GLICK; Fiscal Year: 1990
    ..of human neuroblastoma cells contain a high proportion of fucosyl residues linked alpha 1 leads to 3 to N- acetylglucosamine (Fuc alpha 1 leads to 3G1cNAc) on the oligosaccharide antennae...
  48. N-acetylglucosamine signaling in fungi
    James Konopka; Fiscal Year: 2009
    ..In particular, studies will focus on N-acetylglucosamine (GlcNAc)...
  49. Bordetella Biofilms and Pathogenesis
    RAJENDAR K DEORA; Fiscal Year: 2010
    ..Bps is similar to PGA/PIA/PNAG (poly-2-1,6-N- acetylglucosamine) polysaccharides of other bacteria including a number of pathogens...
  50. N-acetylglucosamine signaling in fungi
    James B Konopka; Fiscal Year: 2010
    ..In particular, studies will focus on N-acetylglucosamine (GlcNAc)...
  51. Bordetella Biofilms and Pathogenesis
    Rajendar Deora; Fiscal Year: 2009
    ..Bps is similar to PGA/PIA/PNAG (poly-2-1,6-N- acetylglucosamine) polysaccharides of other bacteria including a number of pathogens...
  52. MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASES
    JON THORSON; Fiscal Year: 2001
    ....
  53. MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASES
    JON THORSON; Fiscal Year: 2000
    ....
  54. GLYCOSYLATION OF NUCLEAR AND CYTOPLASMIC PROTEINS
    Robert Haltiwanger; Fiscal Year: 1993
    ..Previously described forms of nuclear and cytoplasmic glycosylation, such as O-linked N- acetylglucosamine, have been demonstrated to have novel carbohydrate structures and linkages distinct from classic N- and O-..
  55. MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASES
    JON THORSON; Fiscal Year: 2002
    ....
  56. MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASES
    JON THORSON; Fiscal Year: 1999
    ....
  57. OLIGOSACCHARIDES OF LYSOSOMAL ENZYMES IN SLIME MOLD
    Hudson Freeze; Fiscal Year: 1993
    ..other type of glycosylation is called serine phosphoglycosylation, and it is initiated by the transfer of N-acetylglucosamine-l-phosphate (GlcNAc-alpha-l-P) to serine residues...
  58. Diacylglycerol Kinase: Structure, Folding, and Mechanism
    CHARLES SANDERS; Fiscal Year: 2004
    ..including DG42) are able to synthesize either hyaluronan (a polymer of alternating glucuronic acid and acetylglucosamine residues) or chitin oligosaccharide (containing only acetylglucosamine) depending on conformation of the ..
  59. Diacylglycerol Kinase: Structure, Folding, and Mechanism
    CHARLES SANDERS; Fiscal Year: 2005
    ..including DG42) are able to synthesize either hyaluronan (a polymer of alternating glucuronic acid and acetylglucosamine residues) or chitin oligosaccharide (containing only acetylglucosamine) depending on conformation of the ..
  60. The role of N-glycosylation on beta-sheet protein folding energetics
    JOSHUA L PRICE; Fiscal Year: 2010
    ..This work could ultimately shed light on the relationship between protein glycosylation and protein secretion efficiency, which is related to human lysosomal storage diseases. ..
  61. Diacylglycerol Kinase: Structure, Folding, and Mechanism
    CHARLES SANDERS; Fiscal Year: 2003
    ..including DG42) are able to synthesize either hyaluronan (a polymer of alternating glucuronic acid and acetylglucosamine residues) or chitin oligosaccharide (containing only acetylglucosamine) depending on conformation of the ..
  62. The role of N-glycosylation on beta-sheet protein folding energetics
    Joshua Price; Fiscal Year: 2009
    ..This work could ultimately shed light on the relationship between protein glycosylation and protein secretion efficiency, which is related to human lysosomal storage diseases. ..
  63. Assay development for high throughput screening of selectin ligand antagonists
    PAUL FRENETTE; Fiscal Year: 2006
    ..catalyze the formation of alpha anomeric glycosidic bond between carbon 1 of the fucose and carbon 3 of N-acetylglucosamine. We have developed an ELISA-based assay to evaluate rapidly the FucT activity from cell lysates...
  64. Diacylglycerol Kinase: Structure, Folding, and Mechanism
    CHARLES SANDERS; Fiscal Year: 2002
    ..including DG42) are able to synthesize either hyaluronan (a polymer of alternating glucuronic acid and acetylglucosamine residues) or chitin oligosaccharide (containing only acetylglucosamine) depending on conformation of the ..
  65. DEVELOPMENT AND EXPRESSION OF THE SALIVARY ALG7 GENE
    MARIA KUKURUZINSKA; Fiscal Year: 1993
    ..The ALG7 gene is evolutionarily conserved and essential for growth. It encodes the dolichol-P-dependent N-acetylglucosamine-1-phosphate transferase, GPT, which catalyzes the synthesis of the first lipid-linked intermediate, Dol-PP-..
  66. Assay development for high throughput screening of selectin ligand antagonists
    PAUL FRENETTE; Fiscal Year: 2007
    ..catalyze the formation of alpha anomeric glycosidic bond between carbon 1 of the fucose and carbon 3 of N-acetylglucosamine. We have developed an ELISA-based assay to evaluate rapidly the FucT activity from cell lysates...
  67. Assembly of the mycobacterial cell wall
    Martin Pavelka; Fiscal Year: 2009
    ..Mycobacterial PGN polysaccharide is composed of N-acylmuramic acid and N-acetylglucosamine with peptides (L-alanyl (or glycyl)-D-iso-glutaminyl-meso-diaminopimelyl-D-alanyl-D- alanine) attached to ..