acetylglucosamine

Summary

Summary: The N-acetyl derivative of glucosamine.

Top Publications

  1. ncbi O-linked beta-N-acetylglucosamine (O-GlcNAc): Extensive crosstalk with phosphorylation to regulate signaling and transcription in response to nutrients and stress
    Chutikarn Butkinaree
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Biochim Biophys Acta 1800:96-106. 2010
  2. ncbi Essential role of the glycosyltransferase sxc/Ogt in polycomb repression
    Maria Cristina Gambetta
    Gene Expression Programme, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Science 325:93-6. 2009
  3. ncbi Identification of O-linked N-acetylglucosamine proteins in rat skeletal muscle using two-dimensional gel electrophoresis and mass spectrometry
    Caroline Cieniewski-Bernard
    , Centre National de la Recherche Scientifique 8576, Glycobiologie Structurale et Fonctionnelle, IFR118, Villeneuve d'Ascc, France
    Mol Cell Proteomics 3:577-85. 2004
  4. ncbi Beta-N-acetylglucosamine (O-GlcNAc) is part of the histone code
    Kaoru Sakabe
    Department of Biological Chemistry, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:19915-20. 2010
  5. ncbi LysM, a widely distributed protein motif for binding to (peptido)glycans
    Girbe Buist
    Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    Mol Microbiol 68:838-47. 2008
  6. ncbi In silico modulation of HMGN-1 binding to histones and gene expression by interplay of phosphorylation and O-GlcNAc modification
    Ishtiaq Ahmad
    Institute of Molecular Sciences and Bioinformatics, Lahore, Pakistan
    Protein Pept Lett 15:193-9. 2008
  7. ncbi Increased hexosamine biosynthesis and protein O-GlcNAc levels associated with myocardial protection against calcium paradox and ischemia
    Jia Liu
    Department of Cell Biology and Medicine, University of Alabama at Birmingham, 1530 3rd Avenue South, MCLM 690, Birmingham, AL 35294-0005, USA
    J Mol Cell Cardiol 40:303-12. 2006
  8. ncbi Nutrient sensor O-GlcNAc transferase regulates breast cancer tumorigenesis through targeting of the oncogenic transcription factor FoxM1
    S A Caldwell
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA, USA
    Oncogene 29:2831-42. 2010
  9. ncbi Characterization of a mouse monoclonal antibody specific for O-linked N-acetylglucosamine
    F I Comer
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205-2185, USA
    Anal Biochem 293:169-77. 2001
  10. ncbi Role of protein O-linked N-acetyl-glucosamine in mediating cell function and survival in the cardiovascular system
    Norbert Fülöp
    Department of Medicine, Division of Cardiovascular Disease, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham, AL 35294, USA
    Cardiovasc Res 73:288-97. 2007

Research Grants

  1. PROTEIN-CARBOHYDRATE INTERACTION
    Irwin Goldstein; Fiscal Year: 1990
  2. HTS assay development for inhibitors of sickle cell
    PAUL FRENETTE; Fiscal Year: 2006
  3. CHITIN BIOSYNTHESIS AND THE MOSQUITO PERITROPHIC MATRIX
    Bruce Christensen; Fiscal Year: 2007
  4. Regulation of structure and function of protein by glycosylation
    Zhiwen Zhang; Fiscal Year: 2009
  5. Regulation of structure and function of protein by glycosylation
    Zhiwen Jonathan Zhang; Fiscal Year: 2010
  6. CELL RECEPTORS IN COAGULATION AND ATHEROGENESIS
    SALVATORE PIZZO; Fiscal Year: 1980
  7. Structural and Biochemical Studies of LpxC Inhibition
    Pei Zhou; Fiscal Year: 2009
  8. Structural and Biochemical Studies of LpxC Inhibition
    Pei Zhou; Fiscal Year: 2009
  9. Structural and Biochemical Studies of LpxC Inhibition
    Pei Zhou; Fiscal Year: 2010
  10. Vibrio Cholerae Colonization of Chitin Surfaces
    Gary Schoolnik; Fiscal Year: 2007

Detail Information

Publications188 found, 100 shown here

  1. ncbi O-linked beta-N-acetylglucosamine (O-GlcNAc): Extensive crosstalk with phosphorylation to regulate signaling and transcription in response to nutrients and stress
    Chutikarn Butkinaree
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Biochim Biophys Acta 1800:96-106. 2010
    Since its discovery in the early 1980s, O-linked-beta-N-acetylglucosamine (O-GlcNAc), a single sugar modification on the hydroxyl group of serine or threonine residues, has changed our views of protein glycosylation...
  2. ncbi Essential role of the glycosyltransferase sxc/Ogt in polycomb repression
    Maria Cristina Gambetta
    Gene Expression Programme, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Science 325:93-6. 2009
    ..super sex combs (sxc) encodes Ogt, the highly conserved glycosyltransferase that catalyzes the addition of N-acetylglucosamine (GlcNAc) to proteins in animals and plants...
  3. ncbi Identification of O-linked N-acetylglucosamine proteins in rat skeletal muscle using two-dimensional gel electrophoresis and mass spectrometry
    Caroline Cieniewski-Bernard
    , Centre National de la Recherche Scientifique 8576, Glycobiologie Structurale et Fonctionnelle, IFR118, Villeneuve d'Ascc, France
    Mol Cell Proteomics 3:577-85. 2004
    ..as a nutritional sensor, the level of UDP-GlcNAc that serves as a donor for the uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetyl-glucosaminyltransferase being regulated by the cellular fate of glucose...
  4. ncbi Beta-N-acetylglucosamine (O-GlcNAc) is part of the histone code
    Kaoru Sakabe
    Department of Biological Chemistry, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:19915-20. 2010
    Dynamic posttranslational modification of serine and threonine residues of nucleocytoplasmic proteins by β-N-acetylglucosamine (O-GlcNAc) is a regulator of cellular processes such as transcription, signaling, and protein-protein ..
  5. ncbi LysM, a widely distributed protein motif for binding to (peptido)glycans
    Girbe Buist
    Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    Mol Microbiol 68:838-47. 2008
    ..65 amino acid residues and binds to various types of peptidoglycan and chitin, most likely recognizing the N-acetylglucosamine moiety...
  6. ncbi In silico modulation of HMGN-1 binding to histones and gene expression by interplay of phosphorylation and O-GlcNAc modification
    Ishtiaq Ahmad
    Institute of Molecular Sciences and Bioinformatics, Lahore, Pakistan
    Protein Pept Lett 15:193-9. 2008
    ....
  7. ncbi Increased hexosamine biosynthesis and protein O-GlcNAc levels associated with myocardial protection against calcium paradox and ischemia
    Jia Liu
    Department of Cell Biology and Medicine, University of Alabama at Birmingham, 1530 3rd Avenue South, MCLM 690, Birmingham, AL 35294-0005, USA
    J Mol Cell Cardiol 40:303-12. 2006
    Increased hexosamine biosynthesis pathway (HBP) flux and elevated levels of protein O-linked-N-acetylglucosamine (O-GlcNAc) decrease calcium influx into isolated cardiomyocytes...
  8. ncbi Nutrient sensor O-GlcNAc transferase regulates breast cancer tumorigenesis through targeting of the oncogenic transcription factor FoxM1
    S A Caldwell
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA, USA
    Oncogene 29:2831-42. 2010
    ..a cell's glucose enters the hexosamine biosynthetic pathway (HBP), which regulates levels of O-linked beta-N-acetylglucosamine (O-GlcNAc), a carbohydrate posttranslational modification of diverse nuclear and cytosolic proteins...
  9. ncbi Characterization of a mouse monoclonal antibody specific for O-linked N-acetylglucosamine
    F I Comer
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205-2185, USA
    Anal Biochem 293:169-77. 2001
    beta-O-linked N-acetylglucosamine (O-GlcNAc) is an abundant posttranslational modification of resident nuclear and cytoplasmic proteins in eukaryotes...
  10. ncbi Role of protein O-linked N-acetyl-glucosamine in mediating cell function and survival in the cardiovascular system
    Norbert Fülöp
    Department of Medicine, Division of Cardiovascular Disease, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham, AL 35294, USA
    Cardiovasc Res 73:288-97. 2007
    ....
  11. ncbi Ficolins and the lectin complement pathway
    M Matsushita
    Department of Biochemistry, Fukushima Medical University School of Medicine, Japan
    Immunol Rev 180:78-85. 2001
    ..Recently, it was shown that ficolins present in serum are lectins with a common binding specificity for N-acetylglucosamine (GlcNAc). The fibrinogen-like domain is responsible for the carbohydrate binding...
  12. ncbi Poly-N-acetylglucosamine mediates biofilm formation and detergent resistance in Aggregatibacter actinomycetemcomitans
    Era A Izano
    Department of Oral Biology, New Jersey Dental School, 185 S Orange Avenue, Newark, NJ 07103, USA
    Microb Pathog 44:52-60. 2008
    ..Our findings suggest that PGA, extracellular DNA and proteinaceous adhesins all contribute to the structural integrity of the A. actinomycetemcomitans biofilm matrix...
  13. ncbi Biosynthesis of N-acetylneuraminic acid in cells lacking UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
    S Hinderlich
    , , Berlin-Dahlem, Germany
    Biol Chem 382:291-7. 2001
    ..carbohydrate moiety in biological recognition systems, are performed by the bifunctional enzyme UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase...
  14. ncbi Glucosamine cardioprotection in perfused rat hearts associated with increased O-linked N-acetylglucosamine protein modification and altered p38 activation
    Norbert Fülöp
    Department of Medicine, University of Alabama at Birmingham, 1530 3rd Avenue S, Birmingham, AL 35294 0005, USA
    Am J Physiol Heart Circ Physiol 292:H2227-36. 2007
    ..functional recovery following ischemia and that this appeared to be mediated via an increase in O-linked N-acetylglucosamine (O-GlcNAc) levels on nucleocytoplasmic proteins...
  15. ncbi Impact of Type 2 diabetes and aging on cardiomyocyte function and O-linked N-acetylglucosamine levels in the heart
    Norbert Fülöp
    Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Am J Physiol Cell Physiol 292:C1370-8. 2007
    Increased levels of O-linked attachment of N-acetylglucosamine (O-GlcNAc) on nucleocytoplasmic proteins are implicated in the development of diabetic cardiomyopathy and are regulated by O-GlcNAc transferase (OGT) expression and its ..
  16. ncbi Glutamine-induced protection of isolated rat heart from ischemia/reperfusion injury is mediated via the hexosamine biosynthesis pathway and increased protein O-GlcNAc levels
    Jia Liu
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Mol Cell Cardiol 42:177-85. 2007
    ..cardioprotection could be attributed to increased flux through the HBP and elevated levels of O-linked N-acetylglucosamine (O-GlcNAc) on proteins...
  17. ncbi alpha-Lactalbumin (LA) stimulates milk beta-1,4-galactosyltransferase I (beta 4Gal-T1) to transfer glucose from UDP-glucose to N-acetylglucosamine. Crystal structure of beta 4Gal-T1 x LA complex with UDP-Glc
    B Ramakrishnan
    Structural Glycobiology Section, Laboratory of Experimental and Computational Biology, Center for Cancer Research, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
    J Biol Chem 276:37665-71. 2001
    beta-1,4-Galactosyltransferase 1 (Gal-T1) transfers galactose (Gal) from UDP-Gal to N-acetylglucosamine (GlcNAc), which constitutes its normal galactosyltransferase (Gal-T) activity...
  18. ncbi Mapping of O-linked beta-N-acetylglucosamine modification sites in key contractile proteins of rat skeletal muscle
    Julie Hedou
    Laboratoire de Plasticite Neuromusculaire, Unité de Neurosciences et Physiologies Adaptatives, UPRES EA 4052, IFR 147, Université des Sciences et Technologies de Lille 1, Villeneuve d Ascq, France
    Proteomics 9:2139-48. 2009
    O-linked beta-N-acetylglucosamine (O-GlcNAc) is a widespread modification of serine/threonine residues of nucleocytoplasmic proteins. Recently, several key contractile proteins in rat skeletal muscle (i.e...
  19. ncbi N-acetylglucosamine induces white to opaque switching, a mating prerequisite in Candida albicans
    Guanghua Huang
    Department of Biology, The University of Iowa, Iowa City, Iowa, United States of America
    PLoS Pathog 6:e1000806. 2010
    ..Here, we demonstrate that a second signal, N-acetylglucosamine (GlcNAc), a monosaccharide produced primarily by gastrointestinal tract bacteria, also serves as a potent ..
  20. ncbi The hexosamine signaling pathway: O-GlcNAc cycling in feast or famine
    John A Hanover
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1800:80-95. 2010
    ..It will emphasize the impact O-GlcNAcylation has upon signaling pathways that may be become deregulated in diseases of the immune system, diabetes mellitus, cancer, cardiovascular disease, and neurodegenerative diseases...
  21. ncbi Modification of p53 with O-linked N-acetylglucosamine regulates p53 activity and stability
    Won Ho Yang
    Department of Biology, Yonsei University, 134 Shinchon dong, Seodaemun gu, Seoul 120 749, Korea
    Nat Cell Biol 8:1074-83. 2006
    Post-translational addition of O-linked N-acetylglucosamine (O-GlcNAc) to p53 is known to occur, but the site of O-GlcNAcylation and its effects on p53 are not understood...
  22. ncbi The O-GlcNAc transferase gene resides on the X chromosome and is essential for embryonic stem cell viability and mouse ontogeny
    R Shafi
    The Howard Hughes Medical Institute, Glycobiology Research and Training Center, Department of Cellular and Molecular Medicine, 9500 Gilman Drive 0625, University of California San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 97:5735-9. 2000
    ..Intracellular glycosylation by the addition of N-acetylglucosamine (GlcNAc) to serine and threonine is catalyzed by the O-GlcNAc transferase (OGT)...
  23. ncbi O-GlcNAc signaling attenuates ER stress-induced cardiomyocyte death
    Gladys A Ngoh
    Institute of Molecular Cardiology, University of Louisville, Louisville, Kentucky, USA
    Am J Physiol Heart Circ Physiol 297:H1711-9. 2009
    We previously demonstrated that the O-linked beta-N-acetylglucosamine (O-GlcNAc) posttranslational modification confers cardioprotection at least partially through mitochondrial-dependent mechanisms, but it remained unclear if O-GlcNAc ..
  24. ncbi Production of N-acetyl-D-glucosamine from alpha-chitin by crude enzymes from Aeromonas hydrophila H-2330
    Hitoshi Sashiwa
    Green Biotechnology Research Group, The Special Division for Human Life Technology, National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan
    Carbohydr Res 337:761-3. 2002
    ..The selective and efficient production of N-acetyl-D-glucosamine (GlcNAc) was achieved from flake type of alpha-chitin by using crude enzymes derived from Aeromonas hydrophila H-2330...
  25. ncbi Glucosamine protects neonatal cardiomyocytes from ischemia-reperfusion injury via increased protein O-GlcNAc and increased mitochondrial Bcl-2
    Voraratt Champattanachai
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294 0005, USA
    Am J Physiol Cell Physiol 294:C1509-20. 2008
    ..against ischemia-reperfusion (I/R) injury, and this was associated with an increase in protein O-linked-N-acetylglucosamine (O-GlcNAc) levels...
  26. ncbi O-GlcNAc cycling: how a single sugar post-translational modification is changing the way we think about signaling networks
    Chad Slawson
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Cell Biochem 97:71-83. 2006
    ..This review will focus on recent work involving O-GlcNAc in sensing the environment and regulating signaling cascades...
  27. ncbi Increased enzymatic O-GlcNAcylation of mitochondrial proteins impairs mitochondrial function in cardiac myocytes exposed to high glucose
    Yong Hu
    Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 284:547-55. 2009
    Increased nuclear protein O-linked beta-N-acetylglucosamine glycosylation (O-GlcNAcylation) mediated by high glucose treatment or the hyperglycemia of diabetes mellitus contributes to cardiac myocyte dysfunction...
  28. ncbi Glucosamine protects neonatal cardiomyocytes from ischemia-reperfusion injury via increased protein-associated O-GlcNAc
    Voraratt Champattanachai
    University of Alabama at Birmingham, 1530 3rd Avenue South, MCLM 684, Birmingham, AL 35294 0005, USA
    Am J Physiol Cell Physiol 292:C178-87. 2007
    Increased levels of protein O-linked N-acetylglucosamine (O-GlcNAc) have been shown to increase cell survival following stress...
  29. ncbi Glucosamine improves cardiac function following trauma-hemorrhage by increased protein O-GlcNAcylation and attenuation of NF-{kappa}B signaling
    LuYun Zou
    University of Alabama at Birmingham, Birmingham, AL, USA
    Am J Physiol Heart Circ Physiol 296:H515-23. 2009
    ..function, reduced circulating levels of inflammatory cytokines, and increased tissue levels of O-linked N-acetylglucosamine (O-GlcNAc) on proteins...
  30. ncbi Cross-talk between GlcNAcylation and phosphorylation: roles in insulin resistance and glucose toxicity
    Ronald J Copeland
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205 2185, USA
    Am J Physiol Endocrinol Metab 295:E17-28. 2008
    O-linked beta-N-acetylglucosamine (O-GlcNAc) is a dynamic posttranslational modification that, analogous to phosphorylation, cycles on and off serine and/or threonine hydroxyl groups...
  31. ncbi Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteins
    Gerald W Hart
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205 2185, USA
    Nature 446:1017-22. 2007
    All animals and plants dynamically attach and remove O-linked beta-N-acetylglucosamine (O-GlcNAc) at serine and threonine residues on myriad nuclear and cytoplasmic proteins...
  32. ncbi Metabolic engineering of Escherichia coli for industrial production of glucosamine and N-acetylglucosamine
    Ming De Deng
    Bio Technical Resources, 1035 South 7th Street, Manitowoc, WI 54220, USA
    Metab Eng 7:201-14. 2005
    Glucosamine and N-acetylglucosamine are currently produced by extraction and acid hydrolysis of chitin from shellfish waste...
  33. ncbi The fimbrial adhesin F17-G of enterotoxigenic Escherichia coli has an immunoglobulin-like lectin domain that binds N-acetylglucosamine
    Lieven Buts
    Department of Ultrastructure, Institute for Molecular Biology, Vrije Universiteit Brussel, Vlaams Interuniversitair Instituut voor Biotechnologie, Brussels, Belgium
    Mol Microbiol 49:705-15. 2003
    ..7 A resolution crystal structure of the lectin domain of F17-G, both free and in complex with N-acetylglucosamine. The monosaccharide is bound on the side of the ellipsoid-shaped protein in a conserved site around which ..
  34. ncbi Hemostatic properties of glucosamine-based materials
    Thomas H Fischer
    Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27516, USA
    J Biomed Mater Res A 80:167-74. 2007
    ..The unique natural microalgal fibers were found to have strongly prohemostatic activity compared to the other materials studied...
  35. ncbi Mechanisms of poly-N-acetyl glucosamine polymer-mediated hemostasis: platelet interactions
    Hemant S Thatte
    Department Surgery, VA Boston Healthcare System, Brigham and Women's Hospital, Harvard Medical Schools, Boston, MA, USA
    J Trauma 57:S13-21. 2004
    ..Antibody inhibitors of the platelet alphaIIbbeta3 integrin inhibited p-GlcNAc to stimulate fibrin polymerization. CONCLUSION: Poly-N-acetyl glucosamine fiber material promotes hemostasis by the activation of platelets...
  36. ncbi Ataxin-10 interacts with O-linked beta-N-acetylglucosamine transferase in the brain
    Pia März
    Institute of Physiology, Pestalozzistr 20, University of Basel, CH 4056 Basel, Switzerland
    J Biol Chem 281:20263-70. 2006
    ..O-Linked beta-N-acetylglucosamine transferase (OGT) levels are highest in the brain, and neurodegenerative disorders such as Alzheimer disease ..
  37. ncbi Prevention of leukocyte migration to inflamed skin with a novel fluorosugar modifier of cutaneous lymphocyte-associated antigen
    Charles J Dimitroff
    Harvard Skin Disease Research Center, Department of Dermatology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 112:1008-18. 2003
    ..We have previously shown that a fluorinated analog of N-acetylglucosamine peracetylated-4-fluorinated-d-glucosamine (4-F-GlcNAc), inhibits selectin ligand expression on human T cell ..
  38. ncbi Identification of a dedicated recycling pathway for anhydro-N-acetylmuramic acid and N-acetylglucosamine derived from Escherichia coli cell wall murein
    J T Park
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    J Bacteriol 183:3842-7. 2001
    ..It is demonstrated here that E. coli recycles the N-acetylglucosamine present in cell wall murein degradation products for de novo murein and lipopolysaccharide synthesis...
  39. ncbi Cell signaling, the essential role of O-GlcNAc!
    Natasha E Zachara
    Department of Biological Chemistry, Johns Hopkins Singapore, 31 Biopolis Way, 02-01 The Nanos, 138669 Singapore
    Biochim Biophys Acta 1761:599-617. 2006
    ..Levels of UDP-GlcNAc, and the subsequent addition of O-linked beta-N-acetylglucosamine (O-GlcNAc) to Ser/Thr residues, is involved in regulating nuclear and cytoplasmic proteins in a manner ..
  40. ncbi Caenorhabditis elegans ortholog of a diabetes susceptibility locus: oga-1 (O-GlcNAcase) knockout impacts O-GlcNAc cycling, metabolism, and dauer
    Michele E Forsythe
    Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0850, USA
    Proc Natl Acad Sci U S A 103:11952-7. 2006
    A dynamic cycle of O-linked N-acetylglucosamine (O-GlcNAc) addition and removal acts on nuclear pore proteins, transcription factors, and kinases to modulate cellular signaling cascades...
  41. ncbi Detection of 2-O-sulfated iduronate and N-acetylglucosamine units in heparan sulfate by an antibody selected against acharan sulfate (IdoA2S-GlcNAc)n
    Gerdy B ten Dam
    Department of Biochemistry and Pathology, Nijmegen Center for Molecular Life Sciences, University Medical Center Nijmegen, Nijmegen 6500 HB, The Netherlands
    J Biol Chem 279:38346-52. 2004
    ..This antibody may be instrumental in identifying structural alterations in HS in health and disease...
  42. ncbi Fluorinated per-acetylated GalNAc metabolically alters glycan structures on leukocyte PSGL-1 and reduces cell binding to selectins
    Dhananjay D Marathe
    Chemical and Biological Engineering, State University of New York at Buffalo, Buffalo, NY, USA
    Blood 115:1303-12. 2010
    ..Thus, the compound has pharmacologic activity. Overall, the data suggest that 4F-GalNAc may be applied as a metabolic inhibitor to reduce O-linked glycosylation, sialyl Lewis-X formation, and leukocyte adhesion via the selectins...
  43. ncbi O-linked N-acetylglucosamine is present on the extracellular domain of notch receptors
    Aiko Matsuura
    Department of Applied Molecular Biosciences, Nagoya University Graduate School of Bioagricultural Sciences, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    J Biol Chem 283:35486-95. 2008
    ..Thus, the finding of O-GlcNAc modification in extracellular environments predicts a distinct glycosylation process that might be associated with a novel regulatory mechanism for Notch receptor activity...
  44. ncbi Characterization of ligand binding to N-acetylglucosamine kinase studied by STD NMR
    Astrid Blume
    Institut fur Chemie, Universitat zu Lubeck, Germany
    Biochemistry 47:13138-46. 2008
    Saturation transfer difference (STD) NMR experiments on human N-acetylglucosamine kinase (GlcNAc kinase) have been used to determine binding epitopes for the GlcNAc and ATP substrates and their analogues...
  45. ncbi Protein O-GlcNAcylation: a new signaling paradigm for the cardiovascular system
    Boglarka Laczy
    Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294 0007, USA
    Am J Physiol Heart Circ Physiol 296:H13-28. 2009
    ..residues of nuclear and cytoplasmic proteins by the O-linked attachment of the monosaccharide beta-N-acetylglucosamine (O-GlcNAc) is a highly dynamic and ubiquitous protein modification...
  46. ncbi Evidence of O-linked N-acetylglucosamine in diabetic nephropathy
    Peter Degrell
    2nd Department of Medicine and Nephrological Center, University of Pecs, Hungary
    Life Sci 84:389-93. 2009
    There is increasing evidence that O-linked N-acetylglucosamine (O-GlcNAc) plays an important role in cell signaling pathways...
  47. ncbi Glycosylation of mammalian neurofilaments. Localization of multiple O-linked N-acetylglucosamine moieties on neurofilament polypeptides L and M
    D L Dong
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    J Biol Chem 268:16679-87. 1993
    ..polypeptides are also modified by an abundant type of intracellular protein glycosylation in which single N-acetylglucosamine monosaccharides are O-glycosidically (O-GlcNAc) linked to serine or threonine residues...
  48. ncbi Regulation of heparan sulfate and chondroitin sulfate glycosaminoglycan biosynthesis by 4-fluoro-glucosamine in murine airway smooth muscle cells
    Julie Nigro
    Department of Biomedical Engineering, Lerner Research Institute of The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 284:16832-9. 2009
    ....
  49. ncbi Cardioprotection of salidroside from ischemia/reperfusion injury by increasing N-acetylglucosamine linkage to cellular proteins
    Tiejun Wu
    Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military Medical University, Xi an, China
    Eur J Pharmacol 613:93-9. 2009
    The modification of proteins with O-linked N-acetylglucosamine (O-GlcNAc) is increasingly recognized as an important posttranslational modification that modulates cellular function...
  50. ncbi The role of protein O-linked beta-N-acetylglucosamine in mediating cardiac stress responses
    John C Chatham
    Department of Medicine, Division of Cardiovascular Disease, Center for Free Radical Biology, Center for Aging and Clinical Nutrition Research Center, University of Alabama at Birmingham, Birmingham, AL, USA
    Biochim Biophys Acta 1800:57-66. 2010
    The modification of serine and threonine residues of nuclear and cytoplasmic proteins by O-linked beta-N-acetylglucosamine (O-GlcNAc) has emerged as a highly dynamic post-translational modification that plays a critical role in ..
  51. ncbi Poly-N-acetyl glucosamine nanofibers: a new bioactive material to enhance diabetic wound healing by cell migration and angiogenesis
    Saja Sandra Scherer
    Division of Plastic Surgery, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Ann Surg 250:322-30. 2009
    ..In this study, we developed a polymer, poly-N-acetyl-glucosamine (sNAG), with bioactive properties that may be useful to overcome these limitations...
  52. ncbi Cloning and characterization of the N-acetylglucosamine operon of Escherichia coli
    K G Peri
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada
    Biochem Cell Biol 68:123-37. 1990
    Three enzymes are required for N-acetylglucosamine (NAG) utilization in Escherichia coli: enzyme IInag (gene nagE), N-acetylglucosamine-6-phosphate deacetylase (gene nagA), and glucosamine-6-phosphate isomerase (gene nagB)...
  53. ncbi Characterization of O-acetylation of N-acetylglucosamine: a novel structural variation of bacterial peptidoglycan
    Elvis Bernard
    INRA, UMR1319 MICALIS, F 78350 Jouy en Josas, France
    J Biol Chem 286:23950-8. 2011
    ..We report here the presence of O-acetylation on N-acetylglucosamine (GlcNAc) in Lactobacillus plantarum PG. This modification of glycan strands was never described in bacteria...
  54. ncbi Histidine-regulated activity of M-ficolin
    Michikazu Tanio
    Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo, Japan
    Biochem J 417:485-91. 2009
    ..molecular recognition molecule in the innate immune system, and it binds to some sugars, such as GlcNAc (N-acetylglucosamine), on pathogen surfaces...
  55. ncbi Modification of topoisomerase I activity by glucose and by O-GlcNAcylation of the enzyme protein
    Noa Noach
    Shraga Segal Department of Microbiology and Immunology, Ben Gurion University Cancer Research Center, Faculty of Health Sciences, Ben Gurion University, Beer Sheva, Israel
    Glycobiology 17:1357-64. 2007
    ..Many proteins and enzymes are modified by O-linked beta-N-acetylglucosamine (O-GlcNAc), which exerts profound effects on their function...
  56. ncbi A structural polypeptide of the baculovirus Autographa californica nuclear polyhedrosis virus contains O-linked N-acetylglucosamine
    M Whitford
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Virol 66:3324-9. 1992
    ..germ agglutinin was eliminated by treatment of gp41 with beta-N-acetylglucosaminidase, indicating that N-acetylglucosamine (GlcNAc) was present as terminal residues...
  57. ncbi Skin-homing receptors on effector leukocytes are differentially sensitive to glyco-metabolic antagonism in allergic contact dermatitis
    Madeliene E Gainers
    Harvard Skin Disease Research Center, Department of Dermatology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 179:8509-18. 2007
    ....
  58. ncbi Poly-N-acetyl glucosamine nanofibers regulate endothelial cell movement and angiogenesis: dependency on integrin activation of Ets1
    John N Vournakis
    Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC 29425, USA
    J Vasc Res 45:222-32. 2008
    ....
  59. ncbi Glucose deprivation stimulates O-GlcNAc modification of proteins through up-regulation of O-linked N-acetylglucosaminyltransferase
    Rodrick P Taylor
    Departments of Biochemistry and Medicine, University of Utah School of Medicine, 30 N 2030 East, Salt Lake City, UT 84132, USA
    J Biol Chem 283:6050-7. 2008
    O-Linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of proteins that functions as a nutrient sensing mechanism. Here we report on regulation of O-GlcNAcylation over a broad range of glucose concentrations...
  60. ncbi Binding properties of the N-acetylglucosamine and high-mannose N-glycan PP2-A1 phloem lectin in Arabidopsis
    Julie Beneteau
    UR1268 Unité de Recherches Biopolymères Interactions Assemblages, INRA, F 44300 Nantes, France
    Plant Physiol 153:1345-61. 2010
    ..Using a recombinant protein produced in Escherichia coli, we demonstrated binding to N-acetylglucosamine oligomers...
  61. ncbi Effects of poly-N-acetyl glucosamine (pGlcNAc) patch on wound healing in db/db mouse
    Giorgio Pietramaggiori
    Division of Plastic Surgery, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    J Trauma 64:803-8. 2008
    ..In this study, we hypothesized that a pGlcNAc fiber patch may enhance wound healing in the db/db mouse...
  62. ncbi Expression, essentiality, and a microtiter plate assay for mycobacterial GlmU, the bifunctional glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase
    Wenli Zhang
    Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China
    Int J Biochem Cell Biol 40:2560-71. 2008
    ..Glucosamine-1-phosphate acetyl transferase catalyzes the formation of N-acetylglucosamine-1-phosphate, and N-acetylglucosamine-1-phosphate uridylyltransferase catalyzes the formation of UDP-GlcNAc...
  63. ncbi Parallel identification of O-GlcNAc-modified proteins from cell lysates
    Hwan-Ching Tai
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    J Am Chem Soc 126:10500-1. 2004
    ..The ability to target specific proteins across various tissue or cell types complements emerging proteomic technologies and should advance our understanding of this important posttranslational modification...
  64. ncbi Exploring the O-GlcNAc proteome: direct identification of O-GlcNAc-modified proteins from the brain
    Nelly Khidekel
    Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 101:13132-7. 2004
    The covalent modification of intracellular proteins by O-linked beta-N-acetylglucosamine (O-GlcNAc) is emerging as a crucial regulatory posttranslational modification akin to phosphorylation...
  65. ncbi The superhelical TPR-repeat domain of O-linked GlcNAc transferase exhibits structural similarities to importin alpha
    Martin Jinek
    Structural and Computational Biology, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Struct Mol Biol 11:1001-7. 2004
    Addition of N-acetylglucosamine (GlcNAc) is a ubiquitous form of intracellular glycosylation catalyzed by the conserved O-linked GlcNAc transferase (OGT)...
  66. ncbi Alterations of O-glycosylation, cell wall, and mitochondrial metabolism in Kluyveromyces lactis cells defective in KlPmr1p, the Golgi Ca(2+)-ATPase
    Francesca Farina
    Department of Developmental and Cell Biology, University of Rome La Sapienza, Piazza Aldo Moro 5, 00185 Rome, Italy
    Biochem Biophys Res Commun 318:1031-8. 2004
    ..Alterations in O-glycosylation, consistent with the repression of KlPMT2, were found and a terminal N-acetylglucosamine in the O-glycans was identified...
  67. ncbi Enzymatic synthesis of complex glycosaminotrioses and study of their molecular recognition by hevein domains
    Nuria Aboitiz
    , CSIC, Ramiro de Maeztu 9, Madrid, Spain
    Org Biomol Chem 2:1987-94. 2004
    ....
  68. ncbi Cellular effects of deoxynojirimycin analogues: inhibition of N-linked oligosaccharide processing and generation of free glucosylated oligosaccharides
    Howard R Mellor
    Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Biochem J 381:867-75. 2004
    ..oligosaccharides, of which the majority were glucosylated and had only one reducing terminal GlcNAc (N-acetylglucosamine) residue (FOS-GlcNAc1), demonstrating a cytosolic location...
  69. ncbi Preparation of synthetic glycoconjugates as potential vaccines against Shigella flexneri serotype 2a disease
    Karen Wright
    , URA CNRS 2128, Institut Pasteur, 28 rue du Dr Roux, 75 724, Paris15, France
    Org Biomol Chem 2:1518-27. 2004
    ..Use of thiol-maleimide coupling chemistry allowed site-selective efficient conjugation...
  70. ncbi The importance of including local correlation times in the calculation of inter-proton distances from NMR measurements: ignoring local correlation times leads to significant errors in the conformational analysis of the Glc alpha1-2Glc alpha linkage by NMR
    Mukram Mackeen
    Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Org Biomol Chem 4:2241-6. 2006
    ..The inter-proton distances calculated including the effects of differences in local correlation times give much more consistent results...
  71. ncbi 70-kDa-heat shock protein presents an adjustable lectinic activity towards O-linked N-acetylglucosamine
    Céline Guinez
    UMR 8576 du CNRS, IFR 118, Unite de Glycobiologie Structurale et Fonctionnelle, Batiment C9, Cite Scientifique, 59655 Villeneuve d Ascq cedex, France
    Biochem Biophys Res Commun 319:21-6. 2004
    ..These results strongly suggest that O-GlcNAc influences protein stability through specific interaction with 70-kDa-heat shock protein members...
  72. ncbi Flagellin from Listeria monocytogenes is glycosylated with beta-O-linked N-acetylglucosamine
    M Schirm
    Department of Chemistry, University of Montreal, Montreal, Quebec, Canada
    J Bacteriol 186:6721-7. 2004
    ..monocytogenes is posttranslationally modified with O-linked N-acetylglucosamine (GlcNAc) at up to six sites/monomer...
  73. ncbi Glycan array screening reveals a candidate ligand for Siglec-8
    Bruce S Bochner
    Division of Allergy and Clinical Immunology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
    J Biol Chem 280:4307-12. 2005
    ..We conclude that Siglec-8 binds preferentially to the sLex structure bearing an additional sulfate ester on the galactose 6-hydroxyl...
  74. ncbi Bare-faced curassow lysozyme carrying amino acid substitutions at subsites E and F shows a change in activity against chitooligosaccharide caused by a local conformational change
    Tomohiro Araki
    Department of Bioscience, School of Agriculture, Kyushu Tokai University, Aso, Kumamoto 869 1404, Japan
    J Biochem 136:485-93. 2004
    ..The activity toward the N-acetylglucosamine pentamer revealed that the substitutions at the E-F sites reduced the binding free energies at the E-F sites ..
  75. ncbi Protein ubiquitination is modulated by O-GlcNAc glycosylation
    Céline Guinez
    UMR USTL CNRS 8576, Laboratoire de Glycobiologie Structurale et Fonctionnelle, IFR 147, 59655 Villeneuve d Ascq, France
    FASEB J 22:2901-11. 2008
    ....
  76. ncbi Glycosylation of dodecyl 2-acetamido-2-deoxy-beta-D-glucopyranoside and dodecyl beta-D-galactopyranosyl-(1-->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside as saccharide primers in cells
    Toshinori Sato
    Department of Biosciences and Informatics, Keio University, Yokohama 223 8522, Japan
    Carbohydr Res 343:831-8. 2008
    ..GlcNAc-C12 was also glycosylated by B16 cells and gave sialyllactosamine. Furthermore, LacNAc-C12 gave similar glycosylated products to GlcNAc-C12...
  77. ncbi Dynamic interplay between O-linked N-acetylglucosaminylation and glycogen synthase kinase-3-dependent phosphorylation
    Zihao Wang
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    Mol Cell Proteomics 6:1365-79. 2007
    ..Taken together, these data indicated the complex interplay between phosphorylation and O-GlcNAcylation that occurs within signaling networks...
  78. ncbi Carbohydrate-binding properties of goat secretory glycoprotein (SPG-40) and its functional implications: structures of the native glycoprotein and its four complexes with chitin-like oligosaccharides
    Janesh Kumar
    Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India
    Acta Crystallogr D Biol Crystallogr 63:437-46. 2007
    ..As this appears to essentially be a binding protein, this loss of binding affinity might be compensated by other intermolecular interactions such as protein-protein interactions and also by the binding of its own glycan chain...
  79. ncbi Purification and characterization of Vibrio parahaemolyticus extracellular chitinase and chitin oligosaccharide deacetylase involved in the production of heterodisaccharide from chitin
    K Kadokura
    Department of Biological Chemistry, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252 8510, Japan
    Appl Microbiol Biotechnol 75:357-65. 2007
    ..These findings indicate that GlcNAc-GlcN is produced from chitin by the cooperative hydrolytic reactions of both Pa-Chi and Pa-COD...
  80. ncbi Global methods for protein glycosylation analysis by mass spectrometry
    Bogdan A Budnik
    Department of Pathology, Children's Hospital Boston and Harvard Medical School, Boston, MA 02115, USA
    Biochim Biophys Acta 1764:1870-80. 2006
    ..Finally this review covers aspects of improvements and advances on the instrument front which are important to future global glycoproteomic studies...
  81. ncbi Detection and analysis of proteins modified by O-linked N-acetylglucosamine
    Natasha E Zachara
    The Johns Hopkins University Medical School, Baltimore, Maryland, USA
    Curr Protoc Mol Biol . 2002
    ..This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group...
  82. ncbi Synthesis of beta-(1-->6)-linked glucosamine oligosaccharides corresponding to fragments of the bacterial surface polysaccharide poly-N-acetylglucosamine
    Marina L Gening
    N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky prosp 47, 119991 Moscow, Russia
    Carbohydr Res 342:567-75. 2007
    ..These compounds represent fragments of a bacterial surface polysaccharide produced by numerous bacterial pathogens, including Staphylococcus aureus, and will be used as models for its biochemical and immunological properties...
  83. ncbi Increase in beta1-6 GlcNAc branching caused by N-acetylglucosaminyltransferase V directs integrin beta1 stability in human hepatocellular carcinoma cell line SMMC-7721
    Liying Wang
    Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China
    J Cell Biochem 100:230-41. 2007
    ..Glycosylation caused by GnT-V directs integrin beta1 stability and more delivery to plasma membrane, subsequently promotes Fn-based cell migration and invasion...
  84. ncbi Altered O-glycosylation and sulfation of airway mucins associated with cystic fibrosis
    Baoyun Xia
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Glycobiology 15:747-75. 2005
    ..All mucins contained galactose (Gal), N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc), fucose (Fuc), and sialic acid (Neu5Ac)...
  85. ncbi Oligosaccharide analyses of glycopeptides of horseradish peroxidase by thermal-assisted partial acid hydrolysis and mass spectrometry
    Bao Shiang Lee
    Protein Research Laboratory, Research Resources Center, 835 S Wolcott Avenue, University of Illinois, Chicago, IL 60612, USA
    Carbohydr Res 340:1859-65. 2005
    ..is the fucose (Fuc) and the majority of the end cleavage products are peptides with one or no N-acetylglucosamine (GlcNAc) residue linked to Asparagine (Asn)...
  86. ncbi Does O-GlcNAc play a role in neurodegenerative diseases?
    Tony Lefebvre
    UMR 8576, Centre National de la Recherche Scientifique, Laboratoire de Chimie Biologique, Batiment C9, 59655 Villeneuve d Ascq, France
    Expert Rev Proteomics 2:265-75. 2005
    ..several lines of evidence that the modification of proteins by cytosolic- and nuclear-specific O-linked N-acetylglucosamine (O-GlcNAc) glycosylation is closely related to neuropathologies, particularly Alzheimer's disease...
  87. ncbi Dentin glycoprotein: the protein in the middle of the dentin sialophosphoprotein chimera
    Yasuo Yamakoshi
    University of Michigan Dental Research Laboratory, Ann Arbor, Michigan 48108, USA
    J Biol Chem 280:17472-9. 2005
    ..pentasaccharide core (mannose3-N-acetylglucosamine2), most with a fucosyl residue on the innermost N-acetylglucosamine. The alpha1-3 and alpha1-6 arms are always galactose beta1-4 N-acetylglucosamine beta1-2 mannose, and either ..
  88. ncbi Increased protein O-GlcNAc modification inhibits inflammatory and neointimal responses to acute endoluminal arterial injury
    Dongqi Xing
    Department of Medicine, Univ of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Am J Physiol Heart Circ Physiol 295:H335-42. 2008
    ..This study tested whether increasing protein O-linked-N-acetylglucosamine (O-GlcNAc) levels with glucosamine (GlcN) and O-(2-acetamido-2-deoxy-d-glucopyranosylidene) amino-N-..
  89. ncbi Prolonged incubation in PUGNAc results in increased protein O-Linked glycosylation and insulin resistance in rat skeletal muscle
    Edward B Arias
    Department of Kinesiology, University of Wisconsin Madison, Madison, Wisconsin, USA
    Diabetes 53:921-30. 2004
    Increased flux through the hexosamine biosynthetic pathway and increased O-linked glycosylation (N-acetylglucosamine [O-GlcNAc]) of proteins have been implicated in insulin resistance...
  90. ncbi Site-specific N-glycosylation of human chorionic gonadotrophin--structural analysis of glycopeptides by one- and two-dimensional 1H NMR spectroscopy
    G Weisshaar
    Department of Biochemistry, University of Auckland, New Zealand
    Glycobiology 1:393-404. 1991
    ..predominantly component N2, partially (approximately 25%) and completely alpha 1-6-fucosylated at the N-acetylglucosamine linked to Asn-13 and Asn-30, respectively...
  91. ncbi The high specificities of Phaseolus vulgaris erythro- and leukoagglutinating lectins for bisecting GlcNAc or beta 1-6-linked branch structures, respectively, are attributable to loop B
    Yuko Kaneda
    Tokyo Research and Development, Amersham Biosciences, 3-25-1, Hyakunincho, Shinjuku-ku, Tokyo, 169-0073 Japan
    J Biol Chem 277:16928-35. 2002
    ..Moreover, some of the chimeric lectins prepared in this study showed even higher specificities/affinities than native E(4)- and L(4)-PHA toward complex sugar chains containing either a bisecting GlcNAc residue or a beta1,6-linked branch...
  92. ncbi Development. Carbohydrate recognition in spermatogenesis
    Takashi Muramatsu
    Department of Biochemistry, Nagoya University School of Medicine, Nagoya 466-8550, Japan
    Science 295:53-4. 2002
  93. ncbi Structural basis for selective recognition of oligosaccharides by DC-SIGN and DC-SIGNR
    H Feinberg
    Department of Structural Biology, University School of Medicine, Stanford, CA 94305, USA
    Science 294:2163-6. 2001
    ....
  94. ncbi Cytosolic O-glycosylation is abundant in nerve terminals
    R N Cole
    Department of Biological Chemistry, School of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205 2185, USA
    J Neurochem 79:1080-9. 2001
    ..Here we show that nerve terminal proteins contain another abundant post-translational modification: beta-N-acetylglucosamine linked to hydroxyls of serines or threonines (O-GlcNAc(1))...
  95. ncbi Study of glycosylation with N-trichloroacetyl-D-glucosamine derivatives in the syntheses of the spacer-armed pentasaccharides sialyl lacto-N-neotetraose and sialyl lacto-N-tetraose, their fragments, and analogues
    A A Sherman
    N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, B-334 119991, Moscow, Russia
    Carbohydr Res 336:13-46. 2001
    ..1 equiv for a peracetylated disaccharide one. In the glycosylation products, the N-trichloroacetyl group was easily converted into N-acetyl by alkaline hydrolysis followed by N-acetylation...
  96. ncbi Nucleocytoplasmic O-glycosylation: O-GlcNAc and functional proteomics
    K Vosseller
    Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 N. Wolfe Street, Baltimore, MD 21205, USA
    Biochimie 83:575-81. 2001
    ..The reversible post-translational modification O-linked beta-N-acetylglucosamine (O-GlcNAc) is found on serines and threonines of nuclear and cytoplasmic proteins...
  97. ncbi Down-regulation of the alpha-Gal epitope expression in N-glycans of swine endothelial cells by transfection with the N-acetylglucosaminyltransferase III gene. Modulation of the biosynthesis of terminal structures by a bisecting GlcNAc
    S Koyota
    Department of Biochemistry, Osaka University Medical School, Suita 565-0871, Osaka, Japan
    J Biol Chem 276:32867-74. 2001
    ..The findings may contribute to the development of a novel strategy to eliminate carbohydrate xenoantigens...
  98. ncbi Human alpha3-fucosyltransferases convert chitin oligosaccharides to products containing a GlcNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-4R determinant at the nonreducing terminus
    J Natunen
    Institute of Biotechnology, P.O. Box 56, 00014 University of Helsinki, Finland
    Glycobiology 11:209-16. 2001
    ..experiments; rotating frame nuclear Overhauser spectroscopy data suggested that the fucose and the distal N-acetylglucosamine are stacked in the same way as the fucose and the distal galactose of the Lewis x determinant...
  99. ncbi Glycosylation of nucleocytoplasmic proteins: signal transduction and O-GlcNAc
    L Wells
    Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 USA
    Science 291:2376-8. 2001
    ..The dynamic glycosylation of serine or threonine residues on nuclear and cytosolic proteins by O-linked beta-N-acetylglucosamine (O-GlcNAc) is abundant in all multicellular eukaryotes...
  100. ncbi Glycosylation of the murine estrogen receptor-alpha
    X Cheng
    Department of Biological Chemistry, School of Medicine, Johns Hopkins University, 725 N Wolfe St, Baltimore, MD 21205 2185, USA
    J Steroid Biochem Mol Biol 75:147-58. 2000
    O-linked N-acetylglucosamine (O-GlcNAc) is a highly dynamic and abundant modification found on nuclear and cytoplasmic proteins of nearly all eukaryotes...
  101. ncbi The addition of bisecting N-acetylglucosamine residues to E-cadherin down-regulates the tyrosine phosphorylation of beta-catenin
    T Kitada
    Department of Biochemistry, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
    J Biol Chem 276:475-80. 2001
    The enzyme GnT-III (beta 1,4-N-acetylglucosaminyltransferase III) catalyzes the addition of a bisecting N-acetylglucosamine (GlcNAc) residue on glycoproteins...

Research Grants75

  1. PROTEIN-CARBOHYDRATE INTERACTION
    Irwin Goldstein; Fiscal Year: 1990
    ..The lima bean lectin gene will be cloned and translated; site-specific mutagenesis should allow identification of amino acid residues involved in carbohydrate binding...
  2. HTS assay development for inhibitors of sickle cell
    PAUL FRENETTE; Fiscal Year: 2006
    ..catalyze the formation of an alpha anomeric glycosidic bond between carbon 1 of the fucose and carbon 3 of N-acetylglucosamine. We have developed an ELISA-based assay to evaluate rapidly the FucT activity in cell lysates...
  3. CHITIN BIOSYNTHESIS AND THE MOSQUITO PERITROPHIC MATRIX
    Bruce Christensen; Fiscal Year: 2007
    ..N-acetyltransferase (GNAT), phosphoacetyl glucosamine mutase (AGM), and uridine diphosphate-N-acetylglucosamine pyrophophrylase (UAP) are also involved in this pathway but have never been cloned or characterized in any ..
  4. Regulation of structure and function of protein by glycosylation
    Zhiwen Zhang; Fiscal Year: 2009
    ..We are specifically interested in O-N-acetylglucosamine (O-GlcNAc) modification, where the GlcNAc moiety is attached by an ether linkage to a serine or threonine ..
  5. Regulation of structure and function of protein by glycosylation
    Zhiwen Jonathan Zhang; Fiscal Year: 2010
    ..We are specifically interested in O-N-acetylglucosamine (O-GlcNAc) modification, where the GlcNAc moiety is attached by an ether linkage to a serine or threonine ..
  6. CELL RECEPTORS IN COAGULATION AND ATHEROGENESIS
    SALVATORE PIZZO; Fiscal Year: 1980
    ..be evaluated: the reticuloendothelial system; hepatocyte and macrophage receptors for exposed galactose, N-acetylglucosamine, mannose and fucose; a receptor for clustered positive charges; a unique protease-inhibitor receptor(s)...
  7. Structural and Biochemical Studies of LpxC Inhibition
    Pei Zhou; Fiscal Year: 2009
    ..The committed step of lipid A biosynthesis is catalyzed by UDP-3-O-(acyl)-N- acetylglucosamine deacetylase (LpxC)...
  8. Structural and Biochemical Studies of LpxC Inhibition
    Pei Zhou; Fiscal Year: 2009
    ..The committed step of lipid A biosynthesis is catalyzed by UDP-3-O-(acyl)-N-acetylglucosamine deacetylase (LpxC)...
  9. Structural and Biochemical Studies of LpxC Inhibition
    Pei Zhou; Fiscal Year: 2010
    ..The committed step of lipid A biosynthesis is catalyzed by UDP-3-O-(acyl)-N-acetylglucosamine deacetylase (LpxC)...
  10. Vibrio Cholerae Colonization of Chitin Surfaces
    Gary Schoolnik; Fiscal Year: 2007
    ..Chitin is an insoluble polymer of N-acetylglucosamine; through the secretion of chitinases, V...
  11. MEMBRANE TOPOLOGY AND BIOSYNTHESIS OF GLYCOSAMINOGLYCANS
    CARLOS HIRSCHBERG; Fiscal Year: 1999
    ..Determine the recognition domains for nucleotides, sugars and sulfate of the MDCK transporters for UDP- N-acetylglucosamine and PAPS by using a combination of photolabelling, cross-linking and mutagenesis...
  12. MANNOPROTEIN BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
    Annette Herscovics; Fiscal Year: 1991
    ..oligosaccharides begins with the transfer of a preformed oligosaccharide containing glucose, mannose and N-acetylglucosamine from dolichyl pyrophosphate to asparagine residues in protein...
  13. 'Immunity of Schistosome Glycoconjugates'
    Richard Cummings; Fiscal Year: 2003
    ..These antigenic glycans occur in di- and trisaccharide sequences containing fucose, galactose, xylose, N-acetylglucosamine and N-acetylgalactosamine...
  14. Regulation of IGF-1 and Insulin Signaling by O-GlcNAc Glycosylation
    Lauren E Ball; Fiscal Year: 2010
    ..IRS-1 and IRS-2 are also glycosylated at Ser/Thr residues by N-acetylglucosamine (O-GlcNAc)...
  15. N-acetylglucosamine signaling in fungi
    James B Konopka; Fiscal Year: 2010
    ..In particular, studies will focus on N-acetylglucosamine (GlcNAc)...
  16. Bordetella Biofilms and Pathogenesis
    RAJENDAR K DEORA; Fiscal Year: 2010
    ..Bps is similar to PGA/PIA/PNAG (poly-2-1,6-N- acetylglucosamine) polysaccharides of other bacteria including a number of pathogens...
  17. MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASES
    JON THORSON; Fiscal Year: 2002
    ....
  18. Assay development for high throughput screening of selectin ligand antagonists
    PAUL FRENETTE; Fiscal Year: 2007
    ..catalyze the formation of alpha anomeric glycosidic bond between carbon 1 of the fucose and carbon 3 of N-acetylglucosamine. We have developed an ELISA-based assay to evaluate rapidly the FucT activity from cell lysates...
  19. Diacylglycerol Kinase: Structure, Folding, and Mechanism
    CHARLES SANDERS; Fiscal Year: 2005
    ..including DG42) are able to synthesize either hyaluronan (a polymer of alternating glucuronic acid and acetylglucosamine residues) or chitin oligosaccharide (containing only acetylglucosamine) depending on conformation of the ..
  20. DEVELOPMENT AND EXPRESSION OF THE SALIVARY ALG7 GENE
    MARIA KUKURUZINSKA; Fiscal Year: 1993
    ..The ALG7 gene is evolutionarily conserved and essential for growth. It encodes the dolichol-P-dependent N-acetylglucosamine-1-phosphate transferase, GPT, which catalyzes the synthesis of the first lipid-linked intermediate, Dol-PP-..