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| endostatinsSummarySummary: Angiostatic proteins that are formed from proteolytic cleavage of COLLAGEN TYPE XVIII. Top Publications
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Regulation of collagen-derived antiangiogenic factors by p53Sarah Assadian
McGill University, McGill Cancer Center, 3655 Promenade Sir William Osler, Montreal, QC, Canada
Expert Opin Biol Ther 8:941-50. 2008..This effect is partly mediated by the ability of p53 to increase production of endogenous angiogenesis inhibitors, such as the collagen-derived antiangiogenic factors (CDAFs), endostatin and tumstatin...
Endostatin: an endogenous inhibitor of angiogenesis and tumor growthM S O'Reilly
Department of Surgery, Children s Hospital, Boston, Massachusetts 02115, USA
Cell 88:277-85. 1997..Together with angiostatin data, these findings validate a strategy for identifying endogenous angiogenesis inhibitors, suggest a theme of fragments of proteins as angiogenesis inhibitors, and demonstrate dormancy therapy...
RANKL and vascular endothelial growth factor (VEGF) induce osteoclast chemotaxis through an ERK1/2-dependent mechanismKim Henriksen
Nordic Bioscience, Herlev DK 2730, Denmark
J Biol Chem 278:48745-53. 2003..Taken together, these results show that RANKL and VEGF, in addition to their role in osteoclast differentiation and activation of resorption, are important components of the processes regulating osteoclast chemotaxis...
Angiogenic growth factors and endostatin in non-Hodgkin's lymphomaF Bertolini
Haematology Oncology, IRCCS European Institute of Oncology, Milan, Italy
Br J Haematol 106:504-9. 1999..Our data suggest that b-FGF and, particularly, VEGF might be considered prognostic factors in NHL staging and management...
Gene transfer of matrix metalloproteinase-9 induces tumor regression of breast cancer in vivoChristina Bendrik
Linkoping University, Department of Clinical and Experimental Medicine, Division of Oncology, University Hospital, Linkoping, Sweden
Cancer Res 68:3405-12. 2008..This is the first report showing that overexpression of MMP-9 results in increased generation of antiangiogenic fragments, decreased angiogenesis, and therapeutic effects of established breast cancer...
Adeno-associated virus 2-mediated antiangiogenic cancer gene therapy: long-term efficacy of a vector encoding angiostatin and endostatin over vectors encoding a single factorSelvarangan Ponnazhagan
Department of Pathology, The University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Cancer Res 64:1781-7. 2004....
Continuous administration of endostatin by intraperitoneally implanted osmotic pump improves the efficacy and potency of therapy in a mouse xenograft tumor modelO Kisker
Division of Surgical Research, Children's Hospital, Boston, Massachusetts 02115, USA
Cancer Res 61:7669-74. 2001..On the basis of this approach, an additional clinical trial has been designed and initiated and is under way in two countries...
Adeno-associated virus-mediated gene transfer of endostatin inhibits angiogenesis and tumor growth in vivoWenyin Shi
Department of Pharmacology and Experimental Therapeutics, University of Florida, Gainesville, Florida 32610, USA
Cancer Gene Ther 9:513-21. 2002..This endostatin level was sufficient to inhibit tumor cell-induced angiogenesis and to suppress both the initiation and subsequent growth of a human colorectal cancer model...
Endostar, a novel recombinant human endostatin, exerts antiangiogenic effect via blocking VEGF-induced tyrosine phosphorylation of KDR/Flk-1 of endothelial cellsYun Ling
Department of Physiology, China Pharmaceutical University, Nanjing 210009, China
Biochem Biophys Res Commun 361:79-84. 2007..Collectively, these data indicated the relationship between endostar and VEGF signal pathways and provided a molecular basis for the antiangiogenic effects of endostar...
Inhibition of corneal neovascularization with endostatin delivered by adeno-associated viral (AAV) vector in a mouse corneal injury modelLi Ju Lai
Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Kwei San, Tao Yuan, 333, Taiwan
J Biomed Sci 14:313-22. 2007..We concluded that the rAAV was capable of directly delivering genes to the ocular surface epithelium by way of subconjunctival injection and was able to deliver sustained, high levels of gene expression in vivo to inhibit angiogenesis...
Endostatin inhibits tumour lymphangiogenesis and lymphatic metastasis via cell surface nucleolin on lymphangiogenic endothelial cellsWei Zhuo
National Engineering Laboratory for Anti Tumour Protein Therapeutics, Tsinghua University, Beijing, China
J Pathol 222:249-60. 2010....
Endostatin inhibits adhesion of endothelial cells to collagen I via alpha(2)beta(1) integrin, a possible cause of prevention of chondrosarcoma growthTakayuki Furumatsu
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine and Dentistry, 700-8558, Japan
J Biochem (Tokyo) 131:619-26. 2002..We propose here that endostatin might be effective for anti-angiogenic therapy for human chondrosarcomas through the suppression of alpha(2)beta(1) integrin functions in endothelial cells...
A 27-amino-acid synthetic peptide corresponding to the NH2-terminal zinc-binding domain of endostatin is responsible for its antitumor activityRobert M Tjin Tham Sjin
Vascular Biology Program, Department of Surgery, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA
Cancer Res 65:3656-63. 2005..This peptide contains three histidines that are responsible for zinc binding. Mutations of the zinc-binding histidines abolished its antitumor and antimigration activities, but not antipermeability properties...
High level expression of human endostatin in Pichia pastoris using a synthetic gene constructZhijian Su
School of Pharmaceutical Science, Wenzhou Medical College, Wenzhou, 325035, China
Appl Microbiol Biotechnol 73:1355-62. 2007..pastoris using artificial synthetic gene of preference in P. pastoris was able to inhibit the acidic fibroblast growth factor-induced proliferation of endothelial cells in vitro...
Endostar-loaded PEG-PLGA nanoparticles: in vitro and in vivo evaluationSanyuan Hu
Xiangya School of Medicine, Central South University, Changsha, Hunan Province, People s Republic of China
Int J Nanomedicine 5:1039-48. 2010..Endostar-loaded PEG-PLGA nanoparticles seem to have a better anticancer effect than conventional endostar. We believe that PEG-PLGA nanoparticles are an effective carrier for protein medicines...
Generation and degradation of human endostatin proteins by various proteinasesM Ferreras
OSTEOPRO and Center for Clinical and Basic Research Herlev Ballerup, Herlev, Denmark
FEBS Lett 486:247-51. 2000..None correspond to mouse endostatin. However, the efficiencies of these proteinases differed markedly. Some proteinases also proved to degrade endostatin, pointing to another regulatory loop of angiogenesis...
Endostatin exhibits a direct antitumor effect in addition to its antiangiogenic activity in colon cancer cellsFatima Dkhissi
U553 INSERM, , , , 75475 Paris Cedex 10, France
Hum Gene Ther 14:997-1008. 2003..Thus, endostatin could be used in some colon cancer treatments and its clinical efficacy would depend on the response of tumor cells themselves...
Endostatin associates with integrin alpha5beta1 and caveolin-1, and activates Src via a tyrosyl phosphatase-dependent pathway in human endothelial cellsSara A Wickström
Department of Pathology, Haartman Institute and University of Helsinki, Helsinki, Finland
Cancer Res 62:5580-9. 2002..In addition, the antimigratory effect of endostatin involves phosphatase-dependent Src activation and impaired cell-matrix interactions...
Development of biologic markers of response and assessment of antiangiogenic activity in a clinical trial of human recombinant endostatinRoy S Herbst
Department of Thoracic and Head and Neck Medical Oncology, The University of Texas M D Anderson Cancer Center, Houston, TX, USA
J Clin Oncol 20:3804-14. 2002..We present the initial results of a dose-finding clinical trial of recombinant human endostatin (rh-Endo) that examined potential surrogates for response to antiangiogenic therapy...
Retroviral endostatin gene transfer inhibits human colon cancer cell growth in vivoWeichang Chen
Chin Med J (Engl) 116:1582-4. 2003..01). CONCLUSION: Retroviruses can allow functional expression of the endostatin gene in human colon tumors, showing promise for an antitumor strategy using antiangiogenesis...
Corneal neovascularizationJ H Chang
Schepens Eye Research Institute and the Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114, USA
Curr Opin Ophthalmol 12:242-9. 2001..quot;..
Effect of retroviral endostatin gene transfer on subcutaneous and intraperitoneal growth of murine tumorsA L Feldman
Surgical Metabolism Section, Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
J Natl Cancer Inst 93:1014-20. 2001..Further studies of retroviral endostatin gene transfer for the treatment of cancer are warranted...
Addition of integrin binding sequence to a mutant human endostatin improves inhibition of tumor growthYumi Yokoyama
Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455, USA
Int J Cancer 111:839-48. 2004..These studies demonstrate that addition of a vascular targeting sequence can enhance the biologic activity of an antiangiogenic molecule...
Serum endostatin levels are elevated in patients with soft tissue sarcomaA L Feldman
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Cancer 91:1525-9. 2001..Elevated endostatin levels appear to be associated with tumor aggressiveness. The role of these cytokines in sarcoma angiogenesis and as potential targets for therapy warrants further study...
The synergistic effect of recombinant human endostatin (YH-16) combined with oxaliplatin on human colorectal carcinomaD W Zhang
Department of General Surgery, Harbin Medical University, Harbin, China
J Int Med Res 38:111-26. 2010..No obvious change in side-effects occurred. In conclusion, YH-16 combined with L-OHP inhibited growth and induced apoptosis in orthotopically-transplanted human colorectal carcinoma in nude mice without increasing side-effects...
Recombinant human endostatin-associated acute left heart failureQ Jing
Clin Oncol (R Coll Radiol) 20:268. 2008
Inhibition of human nasopharyngeal carcinoma growth and metastasis in mice by adenovirus-associated virus-mediated expression of human endostatinXiang Ping Li
Department of Chemistry, 8 F Kadoorie Biological Science Building, The University of Hong Kong, Pokfulam, Hong Kong, China
Mol Cancer Ther 5:1290-8. 2006..The potent inhibition of NPC metastasis suggests the feasibility of combining rAAV-hEndo gene therapy with other therapies for the prevention and treatment of NPC metastasis...
A multicenter, randomized, double-blind, placebo-controlled study to evaluate the efficacy of paclitaxel-carboplatin alone or with endostar for advanced non-small cell lung cancerBaohui Han
Shanghai Chest Hospital, Shanghai, China
J Thorac Oncol 6:1104-9. 2011..This study is to explore the clinical efficacy of endostar plus paclitaxel-carboplatin (TC) in advanced NSCLC patients...
Continuous versus intermittent administration of human endostatin in xenografted human neuroblastomaMinoru Kuroiwa
Department of Surgery, Gunma Children's Medical Center, Gunma, Japan
J Pediatr Surg 38:1499-505. 2003..This indicates that rhEndostatin, if administered in continuous fashion, could become an effective agent for treating patients with neuroblastoma in the future...
Targeting cyclooxygenase-2 in recurrent non-small cell lung cancer: a phase II trial of celecoxib and docetaxelIldiko Csiki
Department of Cancer Biology, Vanderbilt-Ingram Cancer Center, Nashville, TN 37232, USA
Clin Cancer Res 11:6634-40. 2005..However, in light of the apparent survival prolongation in the subset with a marked decline in urinary PGE-M levels, further investigation of strategies designed to decrease PGE(2) synthesis in NSCLC seems warranted...
Celecoxib up-regulates the expression of the zeta chain of T cell receptor complex in tumor-infiltrating lymphocytes in human cervical cancerGabriella Ferrandina
Gynecologic Oncology Unit, Department of Histology, Catholic University of Rome, Rome, Italy
Clin Cancer Res 12:2055-60. 2006..Circulating levels of cytokines [interleukin (IL)-1beta, IL-10, tumor necrosis factor-alpha, IL-6, and IL-12] and angiogenesis-modulating factors (vascular endothelial growth factor and endostatin) have also been analyzed...
Experimental inhibition of corneal neovascularization by endostatin gene transfection in vivoPing Zhang
Zhongshan Ophthalmic Center, Sun Yat Sen University, Guangzhou, Chian
Chin Med J (Engl) 116:1869-74. 2003..To investigate endostatin gene therapy of rat corneal neovascularization induced by acid cauterization...
Synergistic antitumor effect of antiangiogenic factor genes on colon 26 produced by low-voltage electroporationMasaya Uesato
Department of Academic Surgery (M9, Graduate School of Medicine, Chiba University 1-8-1, Inohana, Chuou-ku, Chiba-city, Chiba 260-8670, Japan
Cancer Gene Ther 11:625-32. 2004..These data suggest that in vivo delivery of antiangiogenic genes with low-voltage electroporation could be a possible therapeutic strategy for established solid tumors when both genes were applied in combination...
Antitumor activity of endostatin against carcinogen-induced rat primary mammary tumorsG Perletti
Dipartimento di Biologia Strutturale e Funzionale, Universita degli Studi dell Insubria, Varese, Italy
Cancer Res 60:1793-6. 2000..It not only stops the growth of existing tumors but also decreases the incidence of the development of multiple neoplastic lesions...
Effect of medium-chain triglycerides on the release behavior of Endostar encapsulated PLGA microspheresBoyu Meng
Jiangsu Simcere Pharmaceutical R and D Co, Ltd, No 699 18 Wuan Wu Avenue, Nanjing 210042, China
Int J Pharm 397:136-43. 2010....
Characterization of a monoPEG20000-EndostarYue Tong
Department of Biochemistry, China Pharmaceutical University, Nanjing 210009, China
Int J Biol Macromol 46:331-6. 2010..LC/MS was applied to verify the occurrence of mono-PEGylation at the N-terminus of Endostar...
Sustained delivery of endostatin improves the efficacy of therapy in Lewis lung cancer modelJinhui Wu
State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China
J Control Release 134:91-7. 2009..These results indicated PLGA microspheres further reduced the amount of endostatin needed to achieve significant tumor inhibition in mice when compared with systemic administration...
Combination angiostatin and endostatin gene transfer induces synergistic antiangiogenic activity in vitro and antitumor efficacy in leukemia and solid tumors in miceF A Scappaticci
Department of Pathology, Stanford University Medical Center, Stanford, California 94305, USA
Mol Ther 3:186-96. 2001..The data also suggest that novel combinations of antiangiogenic factors delivered into tumors require further investigation as therapeutic modalities...
Synergistic activity of recombinant human endostatin in combination with adriamycin: analysis of in vitro activity on endothelial cells and in vivo tumor progression in an orthotopic murine mammary carcinoma modelStacy M Plum
EntreMed, Inc, Rockville, Maryland 20850, USA
Clin Cancer Res 9:4619-26. 2003..In this study, we determine the antitumor effect of rhEndostatin administered alone or in combination with Adriamycin against established orthotopic murine mammary carcinoma...
Induced repatterning of type XVIII collagen expression in ureter bud from kidney to lung type: association with sonic hedgehog and ectopic surfactant protein CY Lin
Biocenter Oulu and Department of Biochemistry, University of Oulu, Linnanmaa, FIN 90570 Oulu, Finland
Development 128:1573-85. 2001..Our data suggest that differential organ morphogenesis is regulated by an intra-organ patterning process that involves coordination between inductive signals and matrix molecules, such as type XVIII collagen...
Angiogenic and angiostatic factors in systemic sclerosis: increased levels of vascular endothelial growth factor are a feature of the earliest disease stages and are associated with the absence of fingertip ulcersOliver Distler
Center of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, Switzerland
Arthritis Res 4:R11. 2002..VEGF appears to be protective against ischemic manifestations when concentrations of VEGF exceed a certain threshold level...
The minimal active domain of endostatin is a heparin-binding motif that mediates inhibition of tumor vascularizationAnna Karin Olsson
Department of Genetics and Pathology, Uppsala University, Rudbeck Laboratory, Uppsala, Sweden
Cancer Res 64:9012-7. 2004..Substitution of arginine residues 193/194 for alanine attenuates the antiangiogenic effects of the peptide. These data show an essential role for heparin binding in the antiangiogenic action of endostatin...
Endostatin gene transfer inhibits joint angiogenesis and pannus formation in inflammatory arthritisGuoyong Yin
Center for Cardiovascular Research, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, New York 14642, USA
Mol Ther 5:547-54. 2002..These data suggest a novel mechanism by which endostatin inhibits angiogenesis, and demonstrates the potential utility of anti-angiogenic gene therapy for treatment of inflammatory arthritis...
Local endostatin treatment of gliomas administered by microencapsulated producer cellsT A Read
Department of Anatomy and Cell Biology, University of Bergen, Norway
Nat Biotechnol 19:29-34. 2001..The present work may thus provide the basis for new therapeutic approaches toward brain tumors...
Phase II study of recombinant human endostatin in patients with advanced neuroendocrine tumorsMatthew H Kulke
Department of Medical Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
J Clin Oncol 24:3555-61. 2006..We therefore performed a multicenter phase II study of rhEndostatin in patients with carcinoid or pancreatic neuroendocrine tumors...
Early endostatin treatment inhibits metastatic seeding of murine colorectal cancer cells in the liver and their adhesion to endothelial cellsE A te Velde
Department of Surgery, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
Br J Cancer 92:729-35. 2005..Such insights may be helpful in the design of trials to improve (surgical) treatment of colorectal carcinoma and liver metastases...
Tumor hepatocytes and basement membrane-Producing cells specifically express two different forms of the endostatin precursor, collagen XVIII, in human liver cancersO Musso
INSERM U-456, Detoxication and Tissue Repair Unit, Rennes I University, Rennes, France
Hepatology 33:868-76. 2001....
Opposite effects of endostatin on different endothelial cellsAnnette Schmidt
Department of Molecular and Cellular Sport Medicine, German Sport University, Carl-Diem-Weg 6, Cologne 50933, Germany
Cancer Biol Ther 3:1162-6; discussion 1167-8. 2004..Furthermore the cell biological response at lower concentration on angiogenic eESC gives evidence for an angiogenic modulatory rather than a predicted anti-angiogenic role of endostatin...
Pharmacokinetics of His-tag recombinant human endostatin in Rhesus monkeysHai feng Song
Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine, Beijing 100850, China
Acta Pharmacol Sin 26:124-8. 2005..To study the pharmacokinetics and accumulation of an Escherichia coli expressed His-tag fused recombinant human endostatin (rh-endostatin) in Rhesus monkeys...
Corneal transduction to inhibit angiogenesis and graft failureRaghu C Murthy
Clayton Gene Therapy Laboratory, Casey Eye Institute, Oregon Health Sciences University, Portland, Oregon 97201, USA
Invest Ophthalmol Vis Sci 44:1837-42. 2003..E::K-5 gene transduction through a lentiviral vector system may be a useful adjunct to prevent graft neovascularization and corneal graft rejection in high-risk corneal transplants with antecedent rejection or neovascularization...
Elevated serum concentrations of the angiogenesis inhibitor endostatin in preeclamptic womenKora Hirtenlehner
Departments of Obstetrics and Gynecology, University of Vienna, Vienna, Austria
J Soc Gynecol Investig 10:412-7. 2003..The aim of our study was to investigate the concentrations of endostatin, a potent endogenous inhibitor of angiogenesis and endothelial or tumor cell growth, in preeclamptic patients...
The antitumoral effect of endostatin and angiostatin is associated with a down-regulation of vascular endothelial growth factor expression in tumor cellsAmin Hajitou
Laboratory of Tumor and Development Biology, and, Laboratory of Connective Tissues Biology, , Sart-Tilman, , Belgium
FASEB J 16:1802-4. 2002..This work provides evidence that endostatin and angiostatin act on tumor cells themselves...
Endostatin inhibits migration and invasion of head and neck squamous cell carcinoma cellsRalph F Wilson
Departments of Oral Maxillofacial Surgery and Pathology and Periodontology, College of Dentistry, Ohio State University, Columbus, Ohio, USA
Anticancer Res 23:1289-95. 2003..Our data imply that endostatin's clinical efficacy extends beyond angiostatic properties to encompass a direct anti-tumorigenic effect against HNSCC cells...
Intra-arterial delivery of endostatin gene to brain tumors prolongs survival and alters tumor vessel ultrastructureF H Barnett
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Gene Ther 11:1283-9. 2004..We conclude that intra-arterial injection of plasmids selectively targets therapeutic genes to CNS neoplasms. This method of gene therapy holds promise for the treatment of these highly malignant brain tumors...
Endostatin's antiangiogenic signaling networkAmir Abdollahi
Department of Radiation Oncology, German Cancer Research Center (DKFZ, Heidelberg, Germany
Mol Cell 13:649-63. 2004..More generally, insights into the nature of genetic networking from the cell biologic and therapeutic perspectives are revealed...
A polymorphism in the angiogenesis inhibitor, endostatin, in multiple myelomaManoela M Ortega
Leuk Res 27:93-4. 2003
Knobloch syndrome: novel mutations in COL18A1, evidence for genetic heterogeneity, and a functionally impaired polymorphism in endostatinOlivier Menzel
Division of Medical Genetics, University of Geneva Medical School and University Hospital of Geneva, Geneva, Switzerland
Hum Mutat 23:77-84. 2004..We named the second unmapped locus for Knobloch syndrome KNO2. Mutation analysis excluded COL15A1, a member of the multiplexin collagen subfamily similar to COL18A1, as being responsible for KNO2...
Combined therapy with direct and indirect angiogenesis inhibition results in enhanced antiangiogenic and antitumor effectsAmir Abdollahi
Departments of Radiation Oncology, German Cancer Research Center (DKFZ, Heidelberg, Germany
Cancer Res 63:8890-8. 2003..For future clinical evaluation of the concept, a variety of agents with similar mechanistic properties could be tested...
Human tumstatin and human endostatin exhibit distinct antiangiogenic activities mediated by alpha v beta 3 and alpha 5 beta 1 integrinsAkulapalli Sudhakar
Program in Matrix Biology, Department of Medicine and Cancer Center, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
Proc Natl Acad Sci U S A 100:4766-71. 2003..Collectively, such distinct properties of human tumstatin and human endostatin provide the first insight into their diverse antiangiogenic actions and argue for combining them for targeting tumor angiogenesis...
Matrilysin cleavage of corneal collagen type XVIII NC1 domain and generation of a 28-kDa fragmentH C Lin
Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and the Schepens Eye Research Institute, Boston, Massachusetts, USA
Invest Ophthalmol Vis Sci 42:2517-24. 2001..CONCLUSIONS: Collagen XVIII is present in human cornea. MMP-7 cleaves the collagen XVIII NC1 domain to generate a 28-kDa fragment in the cornea...
Generation of biologically active endostatin fragments from human collagen XVIII by distinct matrix metalloproteasesRitva Heljasvaara
Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, University of Oulu, PO Box 5000, FIN 90014 Oulu, Finland
Exp Cell Res 307:292-304. 2005....
N-terminal modification increases the stability of the recombinant human endostatin in vitroLi Ping Jiang
Jiangsu Simcere Pharmaceutical R and D Co, Ltd, 699 18 Xuan Wu Avenue, Nanjing 210042, People s Republic of China
Biotechnol Appl Biochem 54:113-20. 2009..In the proliferation assay with human umbilical-vein endothelial cells, the zinc-binding and zinc-free endostar samples with extra zinc-binding sites displayed similar inhibiting activities...
Intravital microscopy reveals novel antivascular and antitumor effects of endostatin delivered locally by alginate-encapsulated cellsT A Read
Department of Anatomy and Cell Biology, University of Bergen, 5009 Bergen, Norway
Cancer Res 61:6830-7. 2001..Finally, this work further confirms the new therapeutic concept using alginate cell-encapsulation technology for the localized delivery of therapeutic compounds to central nervous system malignancies...
Endostatin-induced modulation of plasminogen activation with concomitant loss of focal adhesions and actin stress fibers in cultured human endothelial cellsS A Wickström
Cell Biology and Molecular Cancer Biology Laboratories, Biomedicum Helsinki, University of Helsinki, FIN 00014 Helsinki, Finland
Cancer Res 61:6511-6. 2001..Our results suggest that the antiangiogenic activity of endostatin involves the modulation of focal adhesions and actin stress fibers and the down-regulation of the urokinase plasminogen activator system...
Inhibition of tumor growth in mice by endostatin derived from abdominal transplanted encapsulated cellsHuaining Teng
Institute of Biochemistry and Cell Biology, Model Organism Research Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Acta Biochim Biophys Sin (Shanghai) 39:278-84. 2007..These results widen the clinical application of the microencapsulated cell endostatin delivery system in cancer treatment...
Endostatin regulates endothelial cell adhesion and cytoskeletal organizationJohan Dixelius
Department of Genetics and Pathology, Uppsala University, Rudbeck Laboratory, 751 85 Uppsala, Sweden
Cancer Res 62:1944-7. 2002..These data provide a molecular basis both for the lack of effect of endostatin on the normal, quiescent vasculature, and its antagonistic effects on stimulated tumor vessels...
Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivoYing Zhang
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese, Academy of Sciences, Dalian 116023, People s Republic of China
J Biomed Mater Res B Appl Biomater 84:79-88. 2008..The optimized microencapsulation conditions for in vivo implantation are liquid core and 240 microm in diameter. This study provides useful information for antiangiogenic gene therapy to tumors using microencapsulated recombinant cells...
Anti-tumor effect of endostatin mediated by retroviral gene transfer in mice bearing renal cell carcinomaEnia Lúcia Coutinho
Division of Nephrology, Department of Medicine, Universidade Federal de Sao Paulo, SP, Brazil
FASEB J 21:3153-61. 2007..A second mechanism may also be implied in endostatin-dependent tumor regression, associated with tumor infiltration of leukocytes. Besides its antiangiogenic properties, endostatin may be a promising adjuvant to immunotherapy...
Adeno-associated virus-mediated antiangiogenic gene therapy with thrombospondin-1 type 1 repeats and endostatinXuefeng Zhang
Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
Clin Cancer Res 13:3968-76. 2007..We aimed to develop rAAV-mediated antiangiogenic gene therapy delivering endostatin and 3TSR, the antiangiogenic domain of thrombospondin-1...
Endostatin gene therapy enhances the efficacy of paclitaxel to suppress breast cancers and metastases in miceJie Li
Department of General Surgery, Shandong Provincial Qianfoshan Hospital, Jinan, China
J Biomed Sci 15:99-109. 2008..The combinational therapy with endostatin and paclitaxel warrants future investigation as a therapeutic strategy to combat breast cancer...
Endostatin-induced tyrosine kinase signaling through the Shb adaptor protein regulates endothelial cell apoptosisJ Dixelius
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala, Sweden
Blood 95:3403-11. 2000..In conclusion, our data show that endostatin induces tyrosine kinase activity and enhanced apoptosis in FGF-treated endothelial cells...
A high risk of occurrence of sporadic breast cancer in individuals with the 104NN polymorphism of the COL18A1 geneGustavo J Lourenco
Breast Cancer Res Treat 100:335-8. 2006..Our results suggest, for the first time, that the homozygous 104NN polymorphism, even at low frequency, constitutes an important inherited determinant of the disease...
Endostatin reduces vascularization, blood flow, and growth in a rat gliosarcomaDag R Sorensen
Department of Comparative Medicine, Rikshospitalet, University of Oslo, Norway
Neuro Oncol 4:1-8. 2002..This work verifies the general anti-angiogenic and antitumor effects of endostatin and indicates that the protein may also be considered as a treatment strategy for malignant brain tumors...
Endostatin competes with bFGF for binding to heparin-like glycosaminoglycansRenata C M Reis
Department of Histology and Embryology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Biochem Biophys Res Commun 333:976-83. 2005..Taken as a whole, our results demonstrate that there is a direct connection between the dependence of endostatin activity on heparin-like glycosaminoglycans and its ability to antagonize bFGF...
Endostatin phenylalanines 31 and 34 define a receptor binding siteSonja Stahl
Department of Human Genetics, , Biozentrum, Am Hubland, , Germany
Genes Cells 10:929-39. 2005..Our data suggest that residues F31 and F34 form a putative receptor binding site acting independently from heparan sulfate binding and that the A48T mutation destabilizes the endostatin molecule...
Clinical significance of plasma endostatin in acute myeloid leukemia/myelodysplastic syndromeRaymond Lai
Department of Hematopathology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA
Cancer 94:14-7. 2002..The mechanism underlying the association between high PE and poor clinical outcome is unclear, although it may be related to the possible PE reflection of tumor burden...
Endostatin causes G1 arrest of endothelial cells through inhibition of cyclin D1Jun Ichi Hanai
Divisions of Nephrology and Hematology Oncology, Department of Medicine and Center for Study of the Tumor Microenvironment, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
J Biol Chem 277:16464-9. 2002..Collectively, our data pointed to a role for cyclin D1, and in particular, transcription through the LEF1 site as critical for endostatin action in vitro and suggest that beta-catenin is a target for endostatin...
Antiangiogenic therapy of experimental cancer does not induce acquired drug resistanceT Boehm
Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 390:404-7. 1997..An unexpected finding is that repeated cycles of antiangiogenic therapy are followed by prolonged tumour dormancy without further therapy...
High serum endostatin levels in Down syndrome: implications for improved treatment and prevention of solid tumoursT S Zorick
Center for the Study of the Human Genome, Department of Biology, University of Sao Paulo, Sao Paulo, Brazil
Eur J Hum Genet 9:811-4. 2001..Based upon these data, we propose that an increase of about one-third of normal endostatin serum levels may represent an effective therapeutic dose to significantly inhibit many solid tumours...
Antiangiogenic activity of restin, NC10 domain of human collagen XV: comparison to endostatinR Ramchandran
Renal Division, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
Biochem Biophys Res Commun 255:735-9. 1999..A polyclonal antibody raised against endostatin cross-reacted with restin. Systemic administration of restin suppressed the growth of tumors in a xenograft renal carcinoma model...
Interaction of endostatin with integrins implicated in angiogenesisM Rehn
Cancer Research Center, The Burnham Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 98:1024-9. 2001..Taken together, these results implicate integrins as potential targets for endostatin function and support the importance of integrins in endothelial cell biology and angiogenesis...
Antiangiogenic activity of a matrix proteinJudah Folkman
Harvard Medical School; Children's Hospital; Hunnelwell 103; 300 Longwood Ave.; Boston, Massachusetts 02115-5737, USA
Cancer Biol Ther 2:53-4. 2003
Angiogenesis and cancer: recent advancesWilliam Caceres
San Juan Veterans Affairs Medical Center, 10 Calle Casia, San Juan, Puerto Rico 00921
P R Health Sci J 22:149-51. 2003..The use of antiangiogenic agents, both in combination with other treatment modalities in the acute setting as well as long-term maintenance or prevention of cancer, is at present one of the better promises in the war against cancer...
Angiogenesis as a target for cancer therapyKerim Kaban
Department of Thoracic Head and Neck Medical Oncology, University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA
Hematol Oncol Clin North Am 16:1125-71. 2002..Still, there is much to learn about the machinery that tumors use to recruit new blood vessels, and the results of the clinical trials will show the best way to apply that knowledge for cancer therapy...
Delivery of endostatin in experimental cancer therapyDag R Sorensen
Department of Comparative Medicine, Rikshospitalet, University of Oslo, Norway
Int J Exp Pathol 83:265-74. 2002..In this paper we review some of the basic knowledge of endostatin and look specifically into the different possible ways in which endostatin may be administered...
Studies on the structure-activity relationship of endostatin: synthesis of human endostatin peptides exhibiting potent antiangiogenic activitiesFrancesco Chillemi
Department of Organic and Industrial Chemistry, University of Milan, Via Venezian, 21, 20133 Milano, Italy
J Med Chem 46:4165-72. 2003..We propose to use the peptides 6-49 and 134-178 as angiogenesis inhibitors in substitution of full length endostatin, in therapeutic applications for cancer, rheumatoid arthritis, and retinopathies...
Functional characterization of neostatins, the MMP-derived, enzymatic cleavage products of type XVIII collagenJin Hong Chang
Corneal, External Disease and Refractive Surgery Service, Massachusetts Eye and Ear Infirmary and the Schepens Eye Research Institute, Harvard Medical School, 243 Charles Street, Boston, MA 02114, United States
FEBS Lett 579:3601-6. 2005..These results provide supportive evidence of the possible anti-angiogenic effect of neostatins...
Adenoviral vectors expressing human endostatin-angiostatin and soluble Tie2: enhanced suppression of tumor growth and antiangiogenic effects in a prostate tumor modelSudhanshu P Raikwar
Department of Urology, Indiana University School of Medicine, 535 Barnhill Drive, Indianapolis, IN 46202, USA
Mol Ther 12:1091-100. 2005..These results suggest that a combinatorial antiangiogenic gene therapy approach involving Ad-hEndo-angio and Ad-sTie2 could become a novel form of treatment for localized human prostate cancer...
Angiogenic and antiangiogenic balance regulates concomitant antitumoral resistanceO Graciela Scharovsky
, School of Medical Sciences, University of Rosario, Rosario, Argentina
Clin Exp Metastasis 21:177-83. 2004..The levels of the factors involved in this phenomenon can vary in the different tumor models, but the trend favoring the inhibition of angiogenesis is always conserved...
The clinical potential of antiangiogenic fragments of extracellular matrix proteinsA R Clamp
Cancer Research UK Department of Medical Oncology, Christie Hospital, Wilmslow Road, Manchester M20 4BX, UK
Br J Cancer 93:967-72. 2005..Lessons learned from these studies will aid the future clinical development of other antiangiogenic extracellular matrix protein fragments...
p53-mediated inhibition of angiogenesis through up-regulation of a collagen prolyl hydroxylaseJose G Teodoro
Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
Science 313:968-71. 2006..Our results reveal a genetic and biochemical linkage between the p53 tumor suppressor pathway and the synthesis of antiangiogenic collagen fragments...
A gene transfer comparative study of HSA-conjugated antiangiogenic factors in a transgenic mouse model of metastatic ocular cancerE Frau
CNRS UMR 8121 Univ Paris Sud, Vectorologie et Transfert de Gènes, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94805 Villejuif, France
Cancer Gene Ther 14:251-61. 2007..Our study indicates that systemic antiangiogenic factors production by recombinant adenoviruses, particularly Can, might represent an effective way of delaying metastatic growth via inhibition of angiogenesis...
Evidence of efficacy: researchers investigating markers for angiogenesis inhibitorsVicki Brower
J Natl Cancer Inst 95:1425-7. 2003
[The clinical perspective of angiogenesis inhibitors]S A Radema
Universitair Medisch Centrum Utrecht, afd. Medische Oncologie, Postbus 85.500, 3508 GA Utrecht
Ned Tijdschr Geneeskd 147:1675-80. 2003..Angiogenesis research has contributed to the knowledge of the biology of cancer, the design of modified clinical studies and the development of surrogate markers that can be used as pharmacodynamic end points in future studies...
[Clinical development of anti-angiogenic agents in 2002]Thomas Bachelot
Unité Cytokines et Cancers, INSERM U453, Centre Leon Berard, 28, rue Laennec, 69373 Lyon Cedex 08
Bull Cancer 90:19-23. 2003..Further clinical trials are needed to gain clear insight into the precise role of those promising strategies for the management of solid tumours...
Angiostatin and endostatin inhibit endothelial cell migration in response to FGF and VEGF without interfering with specific intracellular signal transduction pathwaysKerstin Eriksson
Department of Genetics and Pathology, Uppsala University, Rudbeck Laboratory, S-751 85 Uppsala, Sweden
FEBS Lett 536:19-24. 2003..We conclude that angiostatin and endostatin inhibit chemotaxis, without affecting intracellular signalling pathways known to regulate endothelial migration and proliferation/survival...
Continuous intravascular secretion of endostatin in mice from transduced hematopoietic stem cellsRobert Pawliuk
Division of Health Sciences and Technology, Harvard-MIT, Cambridge, Massachusetts 02139, USA
Mol Ther 5:345-51. 2002..Resolution of this paradox may come from human trials of endostatin now underway...
Adipose tissue mass can be regulated through the vasculatureMaria A Rupnick
Division of Cardiovascular Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 99:10730-5. 2002..We conclude that adipose tissue mass is sensitive to angiogenesis inhibitors and can be regulated by its vasculature...
Kinetics and viability of circulating endothelial cells as surrogate angiogenesis marker in an animal model of human lymphomaS Monestiroli
Division of Hematology-Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy
Cancer Res 61:4341-4. 2001..Conversely, in mice given endostatin, all of the increase in apoptotic cells was in the endothelial cell compartment. CEC evaluation is promising as a noninvasive, surrogate angiogenesis marker...
Antiangiogenic and antitumor effects of endostatin on follicular thyroid carcinomaCaisheng Ye
Department of Vascular Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Sun Yat-sen University, Guangzhou, Guangdong 510075, China
Endocrinology 143:3522-8. 2002..By using two different approaches, namely the recombinant endostatin protein and the gene therapy strategy, our study demonstrated that endostatin could be effective in suppressing the growth of human FTC in immunodeficient mice...
Anticancer treatment of endostatin gene therapy by targeting tumor neovasculature in C57/BL miceS Jia
Institute of Microcirculation, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing
Clin Hemorheol Microcirc 23:251-7. 2000..Hence, our results demonstrated the inhibitory effect of ES gene therapy on angiogenesis-dependent tumor growth and metastasis...
Research Grants
- Molecular mechanism of antiangiogenic properties of gold nanoparticlePriyabrata Mukherjee; Fiscal Year: 2010..This multidisciplinary study will integrate expertise in nanoscience and basic science that may lead to novel strategies to treat angiogenesis-dependent disorders. ..
- Tropomyosin in the antiangiogenic activity of HKaKeith McCrae; Fiscal Year: 2007....
- COLLAGEN GENE STRUCTURE AND EXPRESSIONBjorn Reino Olsen; Fiscal Year: 2010....
- COLLAGEN GENE STRUCTURE AND EXPRESSIONBjorn Olsen; Fiscal Year: 2009....
- Gene-Engineered and Targeted Stem Cell Therapy for MyelomaSelvarangan Ponnazhagan; Fiscal Year: 2009..Successful completion of these studies will allow us to initiate phase-1 human clinical trials. ..
- Gene-Engineered and Targeted Stem Cell Therapy for MyelomaSelvarangan Ponnazhagan; Fiscal Year: 2010..Successful completion of these studies will allow us to initiate phase-1 human clinical trials. ..
- Regulation of angiogenic balance by thrombospondin-1Olga Volpert; Fiscal Year: 2004..This approach has the potential to steer clinical trials towards their most effective use alone, combined with conventional chemotherapy, and for prevention, to delay progressive growth of the dormant tumors. ..
- VEGF-dependent and - Independent Anti-angiogenic TherapyCalvin Kuo; Fiscal Year: 2006..Moreover, these humanized receptors will be expressed from regulated adenoviruses to confer an additional degree of safety as a prelude to eventual clinical use. ..
- Mechanism of mutant TIMP-3 induced neovascularizationMichael Tolentino; Fiscal Year: 2004..Specific aim 3: To develop and test gene vectors expressing inhibitors of angiogenesis (angiostatin, endostatin, PEDF) and test their ability to inhibit subretinal neovascularization in the mutant transgenic mouse. ..
- ANTI ANGIOGENIC ACTIVITY OF CLEAVED HMW KININOGENKeith McCrae; Fiscal Year: 2004..These studies should provide new information concerning the physiologic regulation of angiogenesis, and may lead to identification of new antiangiogenic agents. ..
- ANGIOGENESIS INHIBITORS: A MECHANISM OF ACTIONErkki Ruoslahti; Fiscal Year: 2006..This study may provide important information on the mechanism of action of a class of angiogenesis inhibitors, information that may prove helpful in designing clinical trials for these compounds. ..
- QUANTIFYING EFFECTIVENESS OF ANGIOGENIC INHIBITORSPhilip Hahnfeldt; Fiscal Year: 2000....
- TARGETED DELIVERY OF GENES TO ANGIOGENIC VASCULATURERenata Pasqualini; Fiscal Year: 2004..Given that our peptides also target angiogenic vasculature in the retina, these advances are also likely to extend the potential for neovasculature-specific gene therapy targeting to other diseases involving angiogenesis. ..
- Heparin like Glycosaminoglycan OligosaccharidesRam Sasisekharan; Fiscal Year: 2004..Similar technologies for DNA and proteins include things like gene chips that have revolutionized the study of these molecules. We hope advancements, like out technology, will do the same thing for HLGAGs. ..
- TUMOR PHYSIOLOGICAL EFFECTS OF ANGIOGENESIS INHIBITIONWilliam Lee; Fiscal Year: 2002..Favorable and/or antagonistic interactions revealed by these studies can guide their combined clinical use and should elucidate the role of angiogenesis and tumor cell killing in tumor response to radiation therapy. ..
- Inflammation, fibrosis and myocardial function in schlerodermaDorothee Weihrauch; Fiscal Year: 2009..Based on preliminary data we will use an ApoAl mimetic, D-4F, to improve fibrosis, myocardial function and heart failure. ..
- HEPARAN SULFATE PROTEOGLYCANS IN NEURAL DEVELOPMENTGREGORY COLE; Fiscal Year: 2002..Finally, we will analyze the role of collagen XVIII in blood vessel formation during CNS development. ..
