factor v

Summary

Summary: Heat- and storage-labile plasma glycoprotein which accelerates the conversion of prothrombin to thrombin in blood coagulation. Factor V accomplishes this by forming a complex with factor Xa, phospholipid, and calcium (prothrombinase complex). Deficiency of factor V leads to Owren's disease.

Top Publications

  1. ncbi A common genetic variation in the 3'-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis
    S R Poort
    Hemostasis and Thrombosis Research Center, Leiden University Hospital, The Netherlands
    Blood 88:3698-703. 1996
  2. ncbi The mechanism of inactivation of human factor V and human factor Va by activated protein C
    M Kalafatis
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington 05405 0068
    J Biol Chem 269:31869-80. 1994
  3. ncbi Blood group AB and factor V Leiden as risk factors for pre-eclampsia: a population-based nested case-control study
    Leena M Hiltunen
    Department of Hemostasis, Finnish Red Cross Blood Service, Helsinki, Finland
    Thromb Res 124:167-73. 2009
  4. ncbi Varied prevalence of factor V G1691A (Leiden) and prothrombin G20210A single nucleotide polymorphisms among Arabs
    Wassim Y Almawi
    Al Jawhara Center for Molecular Medicine, Genetics and Inherited Diseases, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Bahrain
    J Thromb Thrombolysis 20:163-8. 2005
  5. ncbi Ethnic distribution of factor V Leiden in 4047 men and women. Implications for venous thromboembolism screening
    P M Ridker
    Division of Preventive Medicine, Brigham and Women s Hospital, Boston, MA 02215 1204, USA
    JAMA 277:1305-7. 1997
  6. ncbi Factor V and thrombotic disease: description of a janus-faced protein
    Gerry A F Nicolaes
    Department of Laboratory Medicine, Division of Clinical Chemistry, Lund University, The Wallenberg Laboratory, University Hospital Malmo, Malmo, Sweden
    Arterioscler Thromb Vasc Biol 22:530-8. 2002
  7. ncbi Frequency of factor V, prothrombin and methylenetetrahydrofolate reductase gene variants in preeclampsia
    Angela V D'Elia
    Department of Biomedical Science and Technology, University of Udine, Udine, Italy
    Gynecol Obstet Invest 53:84-7. 2002
  8. ncbi Coagulation factor V and thrombophilia: background and mechanisms
    Kenneth Segers
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    Thromb Haemost 98:530-42. 2007
  9. ncbi Methylenetetrahydrofolate reductase C677T polymorphism and Factor V Leiden variant in Mexican women with preeclampsia/eclampsia
    I P Davalos
    Divisiones de Genética y Medicina Molecular del Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Col Independencia, CMNO, CP 44330, Guadalajara, Jal Mexico
    Blood Cells Mol Dis 35:66-9. 2005
  10. pmc Factor V is an anticoagulant cofactor for activated protein C during inactivation of factor Va
    Thomas J Cramer
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, Calif, USA
    Pathophysiol Haemost Thromb 37:17-23. 2010

Detail Information

Publications328 found, 100 shown here

  1. ncbi A common genetic variation in the 3'-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis
    S R Poort
    Hemostasis and Thrombosis Research Center, Leiden University Hospital, The Netherlands
    Blood 88:3698-703. 1996
    ..Most individuals (87%) with the 20210 A allele are in the highest quartile of plasma prothrombin levels (> 1.15 U/mL). Elevated prothrombin itself also was found to be a risk factor for venous thrombosis...
  2. ncbi The mechanism of inactivation of human factor V and human factor Va by activated protein C
    M Kalafatis
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington 05405 0068
    J Biol Chem 269:31869-80. 1994
    The cleavage of human factor V and human factor Va by human activated protein C (APC) was analyzed in the absence and presence of phospholipid vesicles containing 75% phosphatidylcholine (PC) and 25% phosphatidylserine (PS)...
  3. ncbi Blood group AB and factor V Leiden as risk factors for pre-eclampsia: a population-based nested case-control study
    Leena M Hiltunen
    Department of Hemostasis, Finnish Red Cross Blood Service, Helsinki, Finland
    Thromb Res 124:167-73. 2009
    ..ABO blood group has been associated with thrombotic disorders and pre-eclampsia. We assessed ABO blood group, seven thrombophilia associated polymorphisms, and anti-beta2-glycoprotein I antibodies as risk factors for pre-eclampsia...
  4. ncbi Varied prevalence of factor V G1691A (Leiden) and prothrombin G20210A single nucleotide polymorphisms among Arabs
    Wassim Y Almawi
    Al Jawhara Center for Molecular Medicine, Genetics and Inherited Diseases, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Bahrain
    J Thromb Thrombolysis 20:163-8. 2005
    b>Factor V G1691A (FV-Leiden) and prothrombin (PRT) G20210A single nucleotide polymorphisms (SNPs) are major inherited risk factors of venous thromboembolism...
  5. ncbi Ethnic distribution of factor V Leiden in 4047 men and women. Implications for venous thromboembolism screening
    P M Ridker
    Division of Preventive Medicine, Brigham and Women s Hospital, Boston, MA 02215 1204, USA
    JAMA 277:1305-7. 1997
    To estimate ethnic-specific prevalence rates of factor V Leiden, an inherited defect of hemostasis associated with risk of venous thrombosis.
  6. ncbi Factor V and thrombotic disease: description of a janus-faced protein
    Gerry A F Nicolaes
    Department of Laboratory Medicine, Division of Clinical Chemistry, Lund University, The Wallenberg Laboratory, University Hospital Malmo, Malmo, Sweden
    Arterioscler Thromb Vasc Biol 22:530-8. 2002
    ..In the prothrombinase complex, the activated form of coagulation factor V (FVa) is an essential cofactor to the enzyme-activated factor X (FXa), FXa being virtually ineffective in the ..
  7. ncbi Frequency of factor V, prothrombin and methylenetetrahydrofolate reductase gene variants in preeclampsia
    Angela V D'Elia
    Department of Biomedical Science and Technology, University of Udine, Udine, Italy
    Gynecol Obstet Invest 53:84-7. 2002
    The association between thrombophilic variants (Leiden mutation of the factor V gene, G20210A mutation of the prothrombin gene and C677T polymorphism of the methylenetetrahydrofolate reductase (MTHFR) gene) with preeclampsia was ..
  8. ncbi Coagulation factor V and thrombophilia: background and mechanisms
    Kenneth Segers
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    Thromb Haemost 98:530-42. 2007
    Human coagulation factor V (FV) is an essential coagulation protein with functions in both the pro- and anticoagulant pathways. Failure to express and control FV functions can either lead to bleeding, or to thromboembolic disease...
  9. ncbi Methylenetetrahydrofolate reductase C677T polymorphism and Factor V Leiden variant in Mexican women with preeclampsia/eclampsia
    I P Davalos
    Divisiones de Genética y Medicina Molecular del Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Col Independencia, CMNO, CP 44330, Guadalajara, Jal Mexico
    Blood Cells Mol Dis 35:66-9. 2005
    ..In addition, the polymorphism of gene encoding for Factor V Leiden G1691A is associated with a prothrombotic state in heterozygous subjects...
  10. pmc Factor V is an anticoagulant cofactor for activated protein C during inactivation of factor Va
    Thomas J Cramer
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, Calif, USA
    Pathophysiol Haemost Thromb 37:17-23. 2010
    Coagulation factor V (FV) promotes inactivation of activated factor VIII (FVIIIa) by activated protein C (APC) and protein S...
  11. ncbi Maternal factor V Leiden mutation is associated with HELLP syndrome in Caucasian women
    Sabine Muetze
    Department of Obstetrics and Gynecology, RWTH Aachen University Hospital, Aachen, Germany
    Acta Obstet Gynecol Scand 87:635-42. 2008
    ..HELLP) syndrome is one of the most severe forms of pre-eclampsia we aimed to assess the prevalence of the factor V Leiden, the prothrombin 20210G >A mutation and the methylenetetrahydrofolate reductase (MTHFR) 677C >T ..
  12. ncbi The significance of genetic polymorphisms of factor V Leiden and prothrombin in the preeclamptic Polish women
    Agnieszka Seremak-Mrozikiewicz
    Division of Perinatology and Women s Diseases, Department of Perinatology and Gynecology, Poznan University of Medical Sciences, Poznan, Poland
    J Thromb Thrombolysis 30:97-104. 2010
    ..Inherited thrombophilias, like polymorphism of factor V (FV) Leiden and prothrombin (PTM) are considered to be involved in the PE development...
  13. ncbi Maternal and neonatal outcome of preeclamptic pregnancies: the potential roles of factor V Leiden mutation and 5,10 methylenetetrahydrofolate reductase
    J Rigo
    1st Department of Obstetrics and Gynecology, Semmelweis University Medical School, Budapest, Hungary
    Hypertens Pregnancy 19:163-72. 2000
    To investigate the potential perinatal effects of Factor V Leiden mutation and 5,10 methylenetetrahydrofolate reductase C677T polymorphism in preeclamptic women.
  14. pmc Low plasma levels of tissue factor pathway inhibitor in patients with congenital factor V deficiency
    Connie Duckers
    Department of Biochemistry, Cardiovascular Research Institute Maastricht CARIM, Maastricht University, Maastricht, The Netherlands
    Blood 112:3615-23. 2008
    Severe factor V (FV) deficiency is associated with mild to severe bleeding diathesis, but many patients with FV levels lower than 1% bleed less than anticipated...
  15. ncbi Evidence for an association of the R485K polymorphism in the coagulation factor V gene with severe preeclampsia from screening 35 polymorphisms in 27 candidate genes
    H Watanabe
    Thromb Haemost 86:1594-5. 2001
  16. ncbi Factor V New Brunswick: Ala221Val associated with FV deficiency reproduced in vitro and functionally characterized
    Marten Steen
    Department of Laboratory Medicine, Division of Clinical Chemistry, Lund University, The Wallenberg Laboratory, University Hospital, Malmo, Sweden
    Blood 102:1316-22. 2003
    b>Factor V (FV) deficiency, also known as parahemophilia, is a rare bleeding disorder. Herein we investigate the first reported missense mutation associated with FV deficiency, Ala221Val, assigned as FV New Brunswick...
  17. ncbi Genetic susceptibility to preeclampsia: roles of cytosineto-thymine substitution at nucleotide 677 of the gene for methylenetetrahydrofolate reductase, 68-base pair insertion at nucleotide 844 of the gene for cystathionine beta-synthase, and factor V Leid
    Y J Kim
    Department of Obstetrics and Gynecology, University of Iowa, Iowa City, USA
    Am J Obstet Gynecol 184:1211-7. 2001
    ....
  18. pmc The association of factor V leiden and prothrombin gene mutation and placenta-mediated pregnancy complications: a systematic review and meta-analysis of prospective cohort studies
    Marc A Rodger
    Thrombosis Program, Division of Hematology, Departments of Medicine, Obstetrics and Gynecology and Epidemiology Community Medicine, University of Ottawa, Ottawa, Ontario, Canada
    PLoS Med 7:e1000292. 2010
    b>Factor V Leiden (FVL) and prothrombin gene mutation (PGM) are common inherited thrombophilias...
  19. ncbi The limits of simulation of the clotting system
    R Wagenvoord
    Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, Netherlands
    J Thromb Haemost 4:1331-8. 2006
    ..To investigate in how far successful simulation of a thrombin generation (TG) curve gives information about the underlying biochemical reaction mechanism...
  20. ncbi Severe venous thromboembolism in a young man with Klinefelter's syndrome and heterozygosis for both G20210A prothrombin and factor V Leiden mutations
    Mario Lapecorella
    Centro Emostasi e Trombosi, Dipartimento di Clinica Medica, Immunologia e Malattie Infettive MIDIM, University of Bari School of Medicine, Italy
    Blood Coagul Fibrinolysis 14:95-8. 2003
    ..Further examinations also showed a double heterozygosis for G20210A prothrombin and factor V Leiden mutations...
  21. pmc Combined deficiency of factor V and factor VIII is due to mutations in either LMAN1 or MCFD2
    Bin Zhang
    Life Sciences Institute, Department of Internal Medicine, 210 Washtenaw Ave, Ann Arbor, MI 48109 0650, USA
    Blood 107:1903-7. 2006
    Mutations in LMAN1 (ERGIC-53) or MCFD2 cause combined deficiency of factor V and factor VIII (F5F8D)...
  22. ncbi Combined effect of factor V Leiden and prothrombin 20210A on the risk of venous thromboembolism--pooled analysis of 8 case-control studies including 2310 cases and 3204 controls. Study Group for Pooled-Analysis in Venous Thromboembolism
    J Emmerich
    Hopital Europeen Georges Pompidou, Service de Medecine Vasculaire, Centre Claude Bernard et Laboratoire d Hémostase, Paris, France
    Thromb Haemost 86:809-16. 2001
    b>Factor V Leiden and factor II G20210A mutations are two frequent genetic risk factors involved in venous thromboembolism (VTE)...
  23. pmc Theoretical and experimental study of the D2194G mutation in the C2 domain of coagulation factor V
    M A Miteva
    French National Institute of Health and Medical Research INSERM U428, University Paris V, 75006 Paris, France
    Biophys J 86:488-98. 2004
    Coagulation factor V (FV) is a large plasma glycoprotein with functions in both the pro- and anticoagulant pathways...
  24. ncbi Adjuvant immunization induces high levels of pathogenic antiphospholipid antibodies in genetically prone mice: another facet of the ASIA syndrome
    A Katzav
    Department of Neurology and Sagol Center for Neurosciences, Sheba Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
    Lupus 21:210-6. 2012
    ..Patients with both antiphospholipid antibodies (aPL) and the genetic coagulopathy factor V Leiden (FVL) are frequently found...
  25. ncbi Electrochemical genosensor based on colloidal gold nanoparticles for the detection of Factor V Leiden mutation using disposable pencil graphite electrodes
    Mehmet Ozsoz
    Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, 35100, Bornova, Izmir, Turkey
    Anal Chem 75:2181-7. 2003
    Electrochemical genosensors for the detection of the Factor V Leiden mutation from polymerase chain reaction (PCR) amplicons using the oxidation signal of colloidal gold (Au) is described...
  26. ncbi Combined Factor V and Factor VIII Deficiency
    Marta Spreafico
    A Bianchi Bonomi Hemophilia and Thrombosis Center, Department of Medicine and Medical Specialties, IRCCS Maggiore Hospital, Mangiagalli and Regina Elena Foundation, University of Milan, 20122 Milan, Italy
    Semin Thromb Hemost 35:390-9. 2009
    Combined deficiency of factor V (FV) and factor VIII (FVIII) (F5F8D, or FV+FVIII) is a autosomal recessive bleeding disorder caused by mutations in genes encoding two components of the endoplasmic reticulum (ER)-Golgi intermediate ..
  27. ncbi Prevalence of factor V Leiden mutation and other hereditary thrombophilic factors in Egyptian children with portal vein thrombosis: results of a single-center case-control study
    Hanaa El-Karaksy
    Department of Pediatrics, Faculty of Medicine, Cairo University, Cairo, Egypt
    Ann Hematol 83:712-5. 2004
    ..Our aim was to assess the prevalence of factor V Leiden mutation and other thrombophilic factors as risk factors in the development of PVT in the pediatric age ..
  28. ncbi Absence of factor V Leiden mutation and low prothrombin G 20210 A mutation prevalence in a healthy Moroccan population
    Florence Mathonnet
    Thromb Haemost 88:1073-4. 2002
  29. ncbi Prevalence and association of the factor V Leiden and prothrombin G20210A in healthy subjects and patients with venous thromboembolism
    D Coen
    Clinical Institute of Laboratory Diagnostics, Zagreb University Hospital Center, Zagreb, Croatia
    Croat Med J 42:488-92. 2001
    To determine the prevalences of factor V Leiden and the G20210A mutation in the prothrombin gene (PT20210A) and the frequency of their association in healthy subjects and in patients with venous thromboembolism (VTE).
  30. ncbi Prevalence of factor V Leiden, prothrombin and methylene tetrahydrofolate reductase mutations in women with adverse pregnancy outcomes in Lebanon
    Laila F Zahed
    Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Lebanon
    Am J Obstet Gynecol 195:1114-8. 2006
    The purpose of this study was to determine the prevalence of factor V Leiden, prothrombin, and methylene tetrahydrofolate reductase gene mutations in women with adverse pregnancy outcome compared with women who had uneventful pregnancies.
  31. ncbi Prevalence of factor V Leiden in patients with myocardial infarction and normal coronary angiography
    J Mansourati
    Cardiology Department of Brest, North Hospital of Saint Etienne, France
    Thromb Haemost 83:822-5. 2000
    b>Factor V Leiden is associated with an increased risk of venous thrombosis and myocardial infarction in young women, but not in men in this latter case...
  32. ncbi Prevalence of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations in 200 healthy Jordanians
    Suhair S Eid
    Molecular Laboratory, King Hussein Medical Centre, Jordan
    Clin Lab Sci 17:200-2. 2004
    ..In order to estimate the frequency of the factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations in the Jordanian population, we screened 200 healthy ..
  33. ncbi Prevalence of antiphospholipid antibodies, factor V G1691A (Leiden) and prothrombin G20210A mutations in early and late recurrent pregnancy loss
    Nabil Mtiraoui
    Research Unit of Haematological and Autoimmune Diseases, Faculty of Pharmacy, Monastir, Center University, Tunisia
    Eur J Obstet Gynecol Reprod Biol 119:164-70. 2005
    ..We assessed the prevalence of inherited (FV-Leiden and PRT G20210A), and acquired (anti-PL antibodies) risk factors among habitual aborters in Tunisia...
  34. ncbi Factor V Leiden and prothrombin gene G20210A mutations are uncommon in portal vein thrombosis in India
    Sanjay Sharma
    Department of Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow 226 014, India
    Indian J Gastroenterol 25:236-9. 2006
    ..The cause of thrombosis in these patients remains unclear. We studied the frequency of mutations in genes for coagulation factors V and II (prothrombin) in 61 Indian patients with PVT and 49 healthy control subjects...
  35. ncbi Prevalence of factor V leiden, factor V cambridge, factor II G20210A and methylenetetrahydrofolate reductase C677T mutations in healthy and thrombophilic Serbian populations
    V Djordjevic
    Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia and Montenegro
    Acta Haematol 112:227-9. 2004
  36. ncbi Prevalence of factor V Leiden and prothrombin G20210A mutation in a large French population selected for nonthrombotic history: geographical and age distribution
    Elisabeth Mazoyer
    Hématologie biologique, Hopital Lariboisiere, Paris, France
    Blood Coagul Fibrinolysis 20:503-10. 2009
    ..These polymorphisms confer a very mild hypercoagulable state as shown by the limited increased in basal D-dimers in mutated FV-G1691A populations and only a trend that does not reach statistical significance for FII-G20210A population...
  37. ncbi High incidence of factor V Leiden and prothrombin G20210A in healthy southern Italians
    Gianluca Sottilotta
    Centro Emofilia, Servizio Emostasi e Trombosi, Azienda Ospedaliera Bianchi Melacrino Morelli, Reggio Calabria, Italy
    Clin Appl Thromb Hemost 15:356-9. 2009
    b>Factor V Leiden (FVL) and G20210 prothrombin (FII G20210A) mutations are risk factors for thromboembolism...
  38. ncbi Deep venous thrombosis and thrombophilic mutations in western Iran: association with factor V Leiden
    Zohreh Rahimi
    Medical Biology Research Center, Kermanshah University of Medical Sciences, Iran
    Blood Coagul Fibrinolysis 21:385-8. 2010
    The aim of present study was to investigate the prevalence of factor V Leiden (FVL) c.1691G>A, prothrombin g.20210G>A and methylenetetrahydrofolate reductase (MTHFR) c...
  39. ncbi Prevalence of two thrombophilia predisposing mutations: factor V G1691A (R506Q; Leiden) and prothrombin G20210A, among healthy Lebanese
    Hala Tamim
    Thromb Haemost 88:691-2. 2002
  40. ncbi Allelic frequency of the factor V Leiden mutation and of the pro-thrombin gene 20210A mutation in healthy Tunisian population
    Lobna Bouaziz
    Thromb Haemost 91:824-5. 2004
  41. ncbi Prevalence of angiotensin-converting enzyme, methylenetetrahydrofolate reductase, Factor V Leiden, prothrombin and apolipoprotein E gene polymorphisms in Morocco
    Thierry Paluku They-They
    Laboratoire de Génétique Médicale et Pathologie Moléculaire LGPM, Faculté de Médecine et de Pharmacie Casablanca, 19 rue Tarik Ibn Ziad, Casablanca, Morocco
    Ann Hum Biol 37:767-77. 2010
    ..These genetic factors are involved in several pathways affecting blood pressure regulation, blood coagulation, homocysteine and lipid metabolisms...
  42. ncbi Factor V Leiden G1691A and prothrombin G20210A mutations are common in Tunisia
    C Frere
    J Thromb Haemost 1:2451-2. 2003
  43. ncbi Risk assessment for recurrent venous thrombosis
    Paul Alexander Kyrle
    Department of Medicine I, Medical University of Vienna, Vienna, Austria
    Lancet 376:2032-9. 2010
    ..Analysis of preliminary data suggests that risk assessment can also be refined through integration of prothrombotic coagulation changes and clinical risk factors...
  44. ncbi Evaluation of the association between hereditary thrombophilias and recurrent pregnancy loss: a meta-analysis
    George Kovalevsky
    Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, and Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania Medical Center, Philadelphia, USA
    Arch Intern Med 164:558-63. 2004
    ..Recently, thrombophilias have been implicated as a possible cause. Factor V Leiden (FVL) and prothrombin gene (G20210A) mutations are the most common types of hereditary thrombophilias, but ..
  45. ncbi Factor V Leiden is associated with pre-eclampsia but not with fetal growth restriction: a genetic association study and meta-analysis
    T Dudding
    Hunter Genetics, Hunter New England Health Service, NSW, Australia
    J Thromb Haemost 6:1869-75. 2008
    ..have suggested that there is a relationship between poor pregnancy outcomes and two polymorphisms, one in the factor V gene, the 1691G to A change (rs6025) located on chromosome 1q23 (factor V Leiden, FVL), and the other in the ..
  46. ncbi Malignancies, prothrombotic mutations, and the risk of venous thrombosis
    Jeanet W Blom
    Department of Clinical Epidemiology, Leiden University Medical Center, Leiden, The Netherlands
    JAMA 293:715-22. 2005
    ..Venous thrombosis is a common complication in patients with cancer, leading to additional morbidity and compromising quality of life...
  47. ncbi Factor V Leiden thrombophilia
    Jody Lynn Kujovich
    Northwest Cancer Specialists, Portland, OR 97227, USA
    Genet Med 13:1-16. 2011
    b>Factor V Leiden is a genetic disorder characterized by a poor anticoagulant response to activated Protein C and an increased risk for venous thromboembolism...
  48. ncbi Pregnancy, the postpartum period and prothrombotic defects: risk of venous thrombosis in the MEGA study
    E R Pomp
    Department of Clinical Epidemiology, Leiden University Medical Center, Leiden, The Netherlands
    J Thromb Haemost 6:632-7. 2008
    ..Venous thrombosis is one of the leading causes of maternal morbidity and mortality...
  49. ncbi Risk of recurrent venous thromboembolism in patients with common thrombophilia: a systematic review
    Wai Khoon Ho
    Department of Haematology, Royal Perth Hospital and Centre for Clinical Research Excellence, University of Western Australia, Perth
    Arch Intern Med 166:729-36. 2006
    The 2 most common genetic polymorphisms that predispose to a first episode of venous thromboembolism (VTE) are factor V Leiden (FVL) and prothrombin G20210A...
  50. ncbi Ethnic differences in the association of factor V Leiden mutation and the C677T methylenetetrahydrofolate reductase gene polymorphism with preeclampsia
    Damar Prasmusinto
    Department of Obstetrics and Gynecology, Section of Endocrinology and Reproductive Medicine, University of Bonn, Sigmund Freudstrasse 25, 53127 Bonn, Germany
    Eur J Obstet Gynecol Reprod Biol 112:162-9. 2004
    This case-control study evaluates the association of the factor V Leiden mutation with preeclampsia and potential synergistic effects of the MTHFR-677T and factor V Leiden mutations with regard to disease risk in two different ethnic ..
  51. ncbi Primary habitual abortions are associated with high frequency of factor V Leiden mutation
    M L Wramsby
    Department of Woman and Child Health, Karolinska Institute, Stockholm, Sweden
    Fertil Steril 74:987-91. 2000
    To analyze the prevalence of the mutation G1691A in factor V gene (Leiden mutation), of mutation C677T in the methylenetetrahydrofolate reductase (MTHFR) gene, and of polymorphism in G20210A in the prothrombin gene in women with recurrent ..
  52. ncbi The thrombophilias: well-defined risk factors with uncertain therapeutic implications
    K A Bauer
    From Veterans Affairs Boston Healthcare System, West Roxbury, Massachusetts, USA
    Ann Intern Med 135:367-73. 2001
    Discovery of the factor V Leiden and prothrombin G20210A mutations has greatly increased the percentage of patients in whom venous thrombosis can be attributed to hereditary thrombophilia...
  53. ncbi Prospective evaluation of hemostatic system activation and thrombin potential in healthy pregnant women with and without factor V Leiden
    S Eichinger
    Department of Internal Medicine I, University Vienna, Austria
    Thromb Haemost 82:1232-6. 1999
    ..The risk of venous thrombosis is higher in pregnant women with factor V Leiden (FVL) than in those with wildtype factor V...
  54. ncbi Risk for subsequent venous thromboembolic complications in carriers of the prothrombin or the factor V gene mutation with a first episode of deep-vein thrombosis
    P Simioni
    Department of Medical and Surgical Sciences, Second Chair of Internal Medicine, University Hospital of Padua, Italy
    Blood 96:3329-33. 2000
    Carriers of a mutation in the prothrombin (clotting factor II) or factor V gene have a 2- to 4-fold greater risk for venous thromboembolism than subjects without the mutations...
  55. ncbi Factor V Leiden and prothrombin gene mutation may predispose to paradoxical embolism in subjects with patent foramen ovale
    Vesa Karttunen
    Department of Neurology, Oulu University Central Hospital, Box 25, FIN 90029 Oulu, Finland
    Blood Coagul Fibrinolysis 14:261-8. 2003
    ..We assessed the occurrence of several prothrombotic states (factor V Leiden, prothrombin G20210A, deficiencies in protein S, protein C and antithrombin, lupus anticoagulant, ..
  56. ncbi Prothrombotic mutations as risk factors for cryptogenic ischemic cerebrovascular events in young subjects with patent foramen ovale
    Nicoletta Botto
    CNR Institute of Clinical Physiology, G Pasquinucci Hospital, Massa, Italy
    Stroke 38:2070-3. 2007
    ..We evaluated the prevalence of the 2 most common genetic risk factors for thromboembolism, factor V Leiden (G1691A) and prothrombin G20210A, in young PFO patients who were referred for percutaneous transcatheter ..
  57. ncbi Distinct association of factor V-Leiden and prothrombin G20210A mutations with deep venous thrombosis in Tunisia and Lebanon
    Lobna Bouaziz-Borgi
    Faculty of Pharmacy, University of Monastir, Tunisia
    Am J Hematol 81:641-3. 2006
    b>Factor V G1691A (FV-Leiden) and prothrombin (PRT) G20210A single nucleotide polymorphisms (SNPs) were associated with venous thrombosis among Caucasians...
  58. ncbi Predictive value of factor V Leiden and prothrombin G20210A in adults with venous thromboembolism and in family members of those with a mutation: a systematic review
    Jodi B Segal
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    JAMA 301:2472-85. 2009
    ..Testing for genetic risks for venous thromboembolism (VTE) is common, but the safety and utility of such testing need review...
  59. ncbi Evaluation of factor V Leiden, prothrombin and methylenetetrahydrofolate reductase gene mutations in patients with severe pregnancy complications in northern Finland
    J Jarvenpaa
    Department of Obstetrics and Gynecology, Oulu University Hospital, Oulu, Finland
    Gynecol Obstet Invest 62:28-32. 2006
    Thrombosis in placenta may lead to severe pregnancy complications. Most important inherited thrombophilias are factor V Leiden mutation, prothrombin mutation, and methylenetetrahydrofolate reductase mutation...
  60. ncbi Novel factor V C2-domain mutation (R2074H) in two families with factor V deficiency and bleeding
    I Schrijver
    Department of Pathology, Stanford University School of Medicine, CA 94305, USA
    Thromb Haemost 87:294-9. 2002
    The molecular basis of Factor V deficiency has been defined in few patients only. We report a homozygous nucleotide change (G6395A) in two Tunisian probands with Factor V deficiency and bleeding episodes...
  61. ncbi Coagulation factor V
    Stefano Duga
    Department of Biology and Genetics for Medical Sciences, University of Milan, via Viotti 3 5, 20133 Milan, Italy
    Int J Biochem Cell Biol 36:1393-9. 2004
    ..b>Factor V (FV) takes part in this process as a component of the prothrombinase complex...
  62. ncbi No association between thrombosis and factor V gene polymorphisms in Chinese Han population
    Hu Yanqing
    Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, China
    Thromb Haemost 89:446-51. 2003
    ..And it is significantly linked to a single nucleotide polymorphisms (SNPs) in the coagulation factor V gene that results in the mutations at R506, R306 and HR2 alleles...
  63. ncbi Activated protein C resistance (FV(Leiden)) and thrombosis: factor V mutations causing hypercoagulable states
    Gerry A F Nicolaes
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Post Office Box 616, 6200 MD, Maastricht, The Netherlands
    Hematol Oncol Clin North Am 17:37-61, vi. 2003
    ..on the protein C anticoagulant pathway and the role of activated protein C resistance in thrombotic disease, and they discuss the involvement in thrombosis of mutations other than the Arg506Gln mutation in the gene encoding for factor V.
  64. ncbi Are factor V and prothrombin mutations associated with increased risk of oral cancer?
    Eleftherios Vairaktaris
    Department of Maxillofacial Surgery, University of Athens Medical School, Vas Sofias 93 and Dim Soutsou 1, Athens, Greece
    Anticancer Res 25:2561-5. 2005
    ..Methylenetetrahydrofolate reductase is associated with pathogenesis of both thrombosis and oral cancer. Therefore, a search for a similar association of other thrombosis-related factors with oral cancer is justified...
  65. ncbi Factor V null mutation affecting the Roche LightCycler factor V Leiden assay
    Mani S Mahadevan
    Department of Pathology, University of Virginia, Charlottesville, VA 22908, USA
    Clin Chem 51:1533-5. 2005
  66. ncbi The murine platelet and plasma factor V pools are biosynthetically distinct and sufficient for minimal hemostasis
    Hongmin Sun
    Department of Internal Medicine, Division of Molecular Medicine and Genetics, University of Michigan, Ann Arbor, 48109, USA
    Blood 102:2856-61. 2003
    Coagulation factor V (FV) is a central regulator of the coagulation cascade. Circulating FV is found in plasma and within platelet alpha granules. The specific functions of these distinct FV pools are uncertain...
  67. ncbi Factor V Leiden in central venous catheter-associated thrombosis
    Rob Fijnheer
    Department of Haematology, University Medical Center Utrecht UMCU, Utrecht, The Netherlands
    Br J Haematol 118:267-70. 2002
    ..The factor V Leiden mutation is an important risk factor for deep venous thrombosis and pulmonary embolism...
  68. ncbi Factor V Leiden and prothrombin gene G20210A mutation in children with venous thromboembolism
    Mariana Bonduel
    Servicio de Hematología Oncología, Hospital de Pediatria Prof Dr Juan P Garrahan, Buenos Aires, Argentina
    Thromb Haemost 87:972-7. 2002
    To determine whether factor V Leiden (FVL) and/or prothrombin gene G20210A mutation (PT20210A) are risk factors for venous thromboembolism (VTE) in Argentinean children...
  69. ncbi Activated protein C resistance and lupus anticoagulant activity induced by plasma and purified monospecific human IgG anti-beta2-glycoprotein-I antibodies
    Martha E Viveros
    Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran
    Rev Invest Clin 57:563-71. 2005
    ..We investigated the activated protein C resistance (APCR) phenotype and the lupus anticoagulant (LA), activity induced by anti-beta2-glycoprotein-I (anti-beta2GP-I) antibodies...
  70. ncbi Postpartum seizures after epidural analgesia: a patient with a mutation of the factor V Leiden and prothrombin gene
    Johannes van Nordennen
    Department of Anesthesiology, Academic Hospital Flemish Free University of Brussels, 1090, Brussels, Belgium
    J Clin Anesth 19:549-50. 2007
    ..a transversal venous sinus, the underlying cause being an activated protein C resistance caused by a heterozygote factor V Leiden mutation and heterozygote prothrombin-gene mutation G20210A...
  71. ncbi Prevalence of factor V Leiden and prothrombin G20210A mutations in Chinese patients with deep venous thrombosis and pulmonary embolism
    Z J Jun
    China Japan Union Hospital, Ji Lin University, Changchun, China
    Clin Lab Haematol 28:111-6. 2006
    ..Several genetic risk factors, especially factor V Leiden and prothrombin G20210A mutations have been reported to be related to VTE in Caucasians, but the ..
  72. pmc Basic mechanisms and pathogenesis of venous thrombosis
    Charles T Esmon
    Oklahoma Medical Research Foundation, Howard Hughes Medical Institute, and Departments of Pathology and Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, United States
    Blood Rev 23:225-9. 2009
    ..Among these, factor V Leiden, which renders factor Va resistant to activated protein C, is the most prevalent with approximately 5% of ..
  73. ncbi Singlet oxygen inactivates fibrinogen, factor V, factor VIII, factor X, and platelet aggregation of human blood
    T W Stief
    Institute of Clinical Chemistry, Philipps University, D 35033, Marburg, Germany
    Thromb Res 97:473-80. 2000
    ..Plasmatic coagulation factors sensible to oxidation are fibrinogen, factor V, factor VIII, and factor X with a 50% effective dose of 2-3 mmol/l NaOCl or taurine-chloramine...
  74. pmc Structural basis of thrombin-mediated factor V activation: the Glu666-Glu672 sequence is critical for processing at the heavy chain-B domain junction
    María Angeles Corral-Rodríguez
    Institute for Biomedical Research, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain
    Blood 117:7164-73. 2011
    Thrombin-catalyzed activation of coagulation factor V (FV) is an essential positive feedback reaction within the blood clotting system...
  75. ncbi Thrombin-catalyzed activation of human coagulation factor V
    K Suzuki
    J Biol Chem 257:6556-64. 1982
    Human coagulation factor V was purified from freshly frozen plasma by a method that gave high yields of single chain factor V...
  76. pmc Contribution of amino acid region 659-663 of Factor Va heavy chain to the activity of factor Xa within prothrombinase
    JAMILA HIRBAWI
    Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA
    Biochemistry 49:8520-34. 2010
    ..We have generated factor V molecules in which all residues were mutated to either lysine (factor V(5K)) or alanine (factor V(5A))...
  77. pmc Factor V Leiden and thrombosis in patients with systemic lupus erythematosus: a meta-analysis
    R Kaiser
    Rosalind Russell Medical Research Center for Arthritis, Division of Rheumatology, University of California, San Francisco UCSF, San Francisco, CA 94143, USA
    Genes Immun 10:495-502. 2009
    The aim of this study was to perform a meta-analysis of the association between the factor V Leiden polymorphism (FVL) and thrombosis among patients with systemic lupus erythematosus (SLE) and/or antiphospholipid antibody (aPL) positivity...
  78. ncbi Factor V Leiden and thermolabile methylenetetrahydrofolate reductase gene variants in an East Anglian preeclampsia cohort
    K M O'Shaughnessy
    Clinical Pharmacology Unit, Department of Medicine, University of Cambridge Clinical School, Cambridge, UK
    Hypertension 33:1338-41. 1999
    ..number of candidate genes, and there have been recent reports of positive association with the Leiden variant of factor V and the thermolabile variant of methylenetetrahydrofolate reductase...
  79. ncbi Crystal structure of the LMAN1-CRD/MCFD2 transport receptor complex provides insight into combined deficiency of factor V and factor VIII
    Edvard Wigren
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden
    FEBS Lett 584:878-82. 2010
    ..The few stable mutation variants are found in the binding surface of the complex leading to impaired LMAN1 binding and F5F8D...
  80. ncbi Livedoid vasculiltis associated with a double heterozygous Factor V Leiden and prothrombin G20210A gene mutations
    S Khenifer
    Department of Dermatology, Claude Bernard University, hôpital de l Hôtel dieu, Lyon, France
    Clin Exp Dermatol 34:e811-3. 2009
    ..b>Factor V Leiden mutations have rarely been reported in association with LV...
  81. pmc Factor V Leiden related Budd-Chiari syndrome
    P Deltenre
    Service d Hepatologie, Hopital Beaujon, Clichy, France
    Gut 48:264-8. 2001
    The role of factor V Leiden as a cause of Budd-Chiari syndrome has only recently been described.
  82. ncbi The role of the factor V Leiden mutation in osteonecrosis of the hip
    Charles J Glueck
    1Cholesterol Center, Jewish Hospital of Cincinnati, Cincinnati, OH, USA
    Clin Appl Thromb Hemost 19:499-503. 2013
    We examined the hypothesis that the factor V Leiden (FVL) and G20101A prothrombin gene mutations are commonly associated with hip osteonecrosis...
  83. ncbi A fatal case of malignant atrophic papulosis (Degos' disease) in a man with factor V Leinden mutation and lupus anticoagulant
    Thomas Hohwy
    Department of Dermatology, Aarhus University Hospital, Denmark
    Acta Derm Venereol 86:245-7. 2006
    ..5 years after onset of the disease. Laboratory investigations revealed a mutation of factor V Leiden and the presence of lupus anticoagulant, but no anti-cardiolipin antibodies...
  84. pmc Protease-activated receptor (PAR) 1 and PAR4 differentially regulate factor V expression from human platelets
    Matthew Duvernay
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    Mol Pharmacol 83:781-92. 2013
    ..PAR4-AP-mediated factor V (FV) association with the platelet surface was 1.6-fold greater than for PAR1-AP...
  85. ncbi Factor V Leiden mutation in postthrombotic and non-postthrombotic venous ulcers
    J Hafner
    Department of Dermatology, University Hospital of Zurich, CH 8091 Zurich, Switzerland
    Arch Dermatol 137:599-603. 2001
    To determine the prevalence of the factor V Leiden mutation in patients with postthrombotic and non-postthrombotic venous ulcers.
  86. ncbi Polymorphisms of clotting factors modify the risk for primary intracranial hemorrhage
    J Corral
    The Neurology Division and the Hematology and Medical Oncology Service, Hospital General Universitario, Murcia, Spain
    Blood 97:2979-82. 2001
    ..Genomic polymerase chain reaction was used to analyze the prevalence of 4 polymorphisms: factor V Leiden, prothrombin 20210A, factor VII-323 Del/Ins of a decanucleotide, and factor XIII V34L...
  87. ncbi An online database of mutations and polymorphisms in and around the coagulation factor V gene
    H L Vos
    J Thromb Haemost 5:185-8. 2007
  88. ncbi Prothrombinase complex assembly. Contributions of protein-protein and protein-membrane interactions toward complex formation
    S Krishnaswamy
    Department of Biochemistry, University of Vermont, Burlington 05405
    J Biol Chem 265:3708-18. 1990
    ..Linkage between the two protein-membrane combination events leads to the further stabilization of the complex on the vesicle surface...
  89. pmc Coagulation factor V(A2440G) causes east Texas bleeding disorder via TFPIα
    Lisa M Vincent
    Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA
    J Clin Invest 123:3777-87. 2013
    ..Affected individuals have normal levels of coagulation factor V (FV) activity, but demonstrate inhibition of global coagulation tests...
  90. ncbi Effects of the factor V G1691A mutation and the factor II G20210A variant on the clinical expression of severe hemophilia A in children--results of a multicenter studys
    Karin Kurnik
    Dept of Pediatrics, University Hospital Munich, Germany
    Haematologica 92:982-5. 2007
    ..022). A protective effect of thrombophilic risk factors was shown for ABF (OR [CIs]: 0.7[0.5-0.9]; p=0.02) and the severity of the hemophilic arthropathy (OR [CIs]: 0.06[0.01-0.3]; p=0.0009)...
  91. ncbi A heparin cofactor II mutation (HCII Rimini) combined with factor V Leiden or type I protein C deficiency in two unrelated thrombophilic subjects
    F Bernardi
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Ferrara, Italy
    Thromb Haemost 76:505-9. 1996
    ..The heparin cofactor II gene alteration was associated with, in one patient, the factor V Leiden mutation and, in the other, type I protein C deficiency...
  92. ncbi Can Factor V Leiden and prothrombin G20210A testing in women with recurrent pregnancy loss result in improved pregnancy outcomes?: Results from a targeted evidence-based review
    Linda A Bradley
    Division of Medical Screening and Special Testing, Department of Pathology and Laboratory Medicine, Women and Infants Hospital of Rhode Island, Warren Alpert Medical School of Brown University, Providence, Rhode Island, USA
    Genet Med 14:39-50. 2012
    Women with recurrent pregnancy loss are offered Factor V Leiden (F5) and/or prothrombin G20210A (F2) testing to identify candidates for anticoagulation to improve outcomes...
  93. ncbi Livedoid vasculopathy in a patient with factor V mutation (Leiden)
    T Biedermann
    Department of Dermatology, Ludwig Maximilians University, Munich, Germany
    J Cutan Pathol 27:410-2. 2000
    ..For these forms, an unknown vaso-occlusive or thrombogenic process has been accused to play a role. Thus, a patient with livedoid vasculopathy was examined for different parameters which can be involved in coagulopathies...
  94. ncbi Primer-engineered multiplex PCR-RFLP for detection of MTHFR C677T, prothrombin G20210A and factor V Leiden mutations
    Vedat Koksal
    Burc Molecular Diagnostic Laboratory, Istanbul, Turkey
    Exp Mol Pathol 83:1-3. 2007
    Single-nucleotide polymorphisms in the genes that code for coagulation factors V (factor V Leiden) and II (prothrombin, G20210A), as well as the methylenetetrahydrofolate reductase (MTHFR, C677T) gene, have been implicated in the majority ..
  95. ncbi Endogenous factor V synthesis in megakaryocytes contributes negligibly to the platelet factor V pool
    M Christella L G D Thomassen
    Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    Haematologica 88:1150-6. 2003
    Coagulation factor V (FV) is distributed between two pools: 80% circulates in plasma and 20% is stored in platelets. The aim of the study was to determine the origin of platelet FV.
  96. ncbi Broadening the factor V Leiden paradox: pulmonary embolism and deep-vein thrombosis as 2 sides of the spectrum
    Kirsten van Langevelde
    Department of Clinical Epidemiology, Leiden University Medical Center, Leiden, The Netherlands
    Blood 120:933-46. 2012
    ..A well-known example is the factor V Leiden (FVL) paradox: the FVL mutation poses a clearly higher risk for deep-vein thrombosis (DVT) than for ..
  97. ncbi The R306G and R506Q mutations in coagulation Factor V reveals additional cleavage sites for Activated Protein C in the R313-R321 region and at R505
    Richard J Dirven
    Einthoven Laboratory for Experimental Vascular Medicine, Dept of Thrombosis and Hemostasis, Leiden University Medical Center, Leiden, The Netherlands
    Thromb Res 125:444-50. 2010
    The procoagulant function of activated factor V (FVa) is inhibited by activated Protein C (APC) through proteolytic cleavages at R306, R506 and R679...
  98. ncbi Factor V Leiden and prothrombin G20210A mutations and the risk of atherothrombotic events in systemic lupus erythematosus
    Rudolf Pullmann
    Medical Clinic II, Jessenius Medical Faculty, Martin Faculty Hospital, Martin, Slovakia
    Clin Appl Thromb Hemost 10:233-8. 2004
    ..SLE) remains to be determined, the most common genetic prothrombotic factors, prothrombin G20210A and factor V Leiden mutations, were studied...
  99. ncbi A male adolescent with left iliac thrombophlebitis and heterozygosity for factor V Leiden mutation
    Amy E Lovejoy
    Division of Hematology Oncology, Rady Children s Hospital, San Diego, CA 92123, USA
    J Pediatr Surg 44:1640-2. 2009
    ..J Intern Med. 2002;252:276-280). Secondly, resistance to activated protein C as a result of factor V Leiden is associated with thromboembolic disease at an early age (Price DT, Ridker PM. Ann Intern Med...
  100. ncbi Increased titer of anti-beta2-glycoprotein I IgG antibody among factor V Leiden carriers during oral contraceptive use
    R Poka
    Department of Obstetrics and Gynecology, University of Debrecen, Medical and Health Science Cente, Nagyerdei krt 98, 4012 Debrecen, Hungary
    Contraception 69:27-30. 2004
    The risk of thromboembolism during oral contraceptive (OC) use is increased among factor V Leiden (FVL) carriers compared to women with wild-type genotype of the gene for coagulation factor V (FV)...
  101. ncbi Factor II G20210A and factor V G1691A gene mutations and peripheral arterial occlusive disease
    W Renner
    Department of Medicine, Karl Franzens University, Graz, Austria
    Thromb Haemost 83:20-2. 2000
    G to A mutations at positions 20210 of the prothrombin gene (F2) and 1691 of the factor V gene (F5) are established risk factors for venous thrombosis...

Research Grants64

  1. MOLECULAR BIOLOGY OF HUMAN COAGULATION FACTOR V
    Thomas Ortel; Fiscal Year: 2010
    ..A membrane binding site was localized to the factor V C2 domain and the structures of two crystal forms of this domain have been elucidated...
  2. STRUCTURE AND FUNCTION OF HEPARIN COFACTOR II
    Douglas M Tollefsen; Fiscal Year: 2010
    ..C57BL/6 mice with HCII deficiency in combination with factor V Leiden or protein Z deficiency will be observed for evidence of spontaneous thrombosis or other abnormalities...
  3. Membrane Receptors Regulating Megakaryocyte Endocytosis of Factor V from Plasma
    BETH BOUCHARD; Fiscal Year: 2012
    ..Recently, our laboratory demonstrated that the entire pool of the platelet-derived procofactor, factor V, originates from plasma through endocytosis of factor V by platelet precursors, megakaryocytes, via a two ..
  4. FACTOR VA INTERACTIONS REGULATING PROTHROMBIN ACTIVATION
    Paul E Bock; Fiscal Year: 2010
    b>Factor V plays a pivotal role in hemostasis, and in the pathology of venous thrombosis...
  5. MOLECULAR BASIS OF BLOOD COAGULATION REGULATION
    Steven T Olson; Fiscal Year: 2013
    ..in the case of anti-thrombin and increased thrombosis when mice are given a thrombotic challenge or are bred on a factor V Leiden background in the case of ZPI...
  6. Identifying novel genetic risk factors for venous thromboembolism (VTE)
    Jun Li; Fiscal Year: 2013
    ..However, known common variants, confirmed in recent genome-wide association studies (GWAS), such as Factor V Leiden and the ABO blood group, account for only half of this risk...
  7. MOLECULAR GENETICS OF COAGULATION DISORDERS
    David Ginsburg; Fiscal Year: 2013
    ..this PPG will: (1) continue a whole genome ENU mutagenesis analysis in the mouse to identify genetic modifiers of factor V Leiden, while also taking advantage of natural murine strain variation to identify additional thrombosis ..
  8. TFPI ALPHA AND TFPI BETA
    George J Broze; Fiscal Year: 2013
    ..TFPI1 interacts with protein S (PS), which enhances its anti-FXa activity, and with factor V (FV), which may prolong its clearance from plasma...
  9. Risk-Benefit Framework for Genetic Tests
    David L Veenstra; Fiscal Year: 2010
    ..it to two additional case studies: a) Gene expression profiling in women with early stage breast cancer, and b) Factor V Leiden testing for pregnant women with clotting or adverse pregnancy outcomes;(3) Evaluate and optimize the ..
  10. RISK FACTORS FOR VENOUS THROMBOEMBOLISM IN THE COMMUNITY
    John A Heit; Fiscal Year: 2013
    ..in the observed incidence of VTE;in Aim 2 to determine "when genetic testing is appropriate" by 2a) testing Factor V Leiden, Prothrombin G20210A, and novel ABO SNPs as risk factors for VTE after hospitalization for major surgery ..
  11. Mechanisms of Clotting Factor Assembly
    James H Morrissey; Fiscal Year: 2013
    ..that, in addition to being an extremely potent initiator of the contact pathway, polyphosphate also accelerates factor V activation and enhances fibrin clot structure, leading to thicker fibrin fibrils that are more resistant to ..
  12. Surrogate markers for Severe Pulmonary Embolism
    JEFFREY KLINE; Fiscal Year: 2005
    ..if patients with PE complicated by an adverse outcome are more likely to have antiphospholipid antibodies, or factor V Leiden G1691A, prothrombin G20210A, methylenetetrahydrofolate reductase C677T mutations, or low red blood cell ..
  13. Epidemiologic Study of Placental Abruption
    Cande Ananth; Fiscal Year: 2006
    ..from which DNA will be extracted and assayed for genetic mutations and polymorphisms, including those for factor V Leiden (1691G yields A), prothrombin gene (20210G yields A), the 677C yields T and 1298A yields C polymorphisms ..
  14. Molecular Basis of Procofactor Activation
    Rodney M Camire; Fiscal Year: 2012
    ..The inactive procofactor protein factor V (FV) cannot participate to any significant degree in its macromolecular enzyme complex...
  15. Thrombin Formation Inhibitors
    James Phillips; Fiscal Year: 2004
    ..Generation of DVT is strongly correlated with the function of factor V, a protein cofactor for prothrombinase...
  16. MUTATIONS, HORMONE THERAPY, AND VENOUS THROMBOEMBOLISM
    Bruce Psaty; Fiscal Year: 2001
    DESCRIPTION: (Adapted from Investigator's Abstract) Epidemiologic studies have identified Factor V Leiden as the most common cause of heritable thrombophilia a prothrombotic mutation associated with a 5 to 7-fold increase in the risk of ..
  17. ORAL CONTRACEPTIVES AND THROMBOEMBOLIC DISEASE
    Stephen Sidney; Fiscal Year: 2000
    ..users of low-dose (<50 micrograms estrogen) oral contraceptive (OC) preparations; and b) the prevalence of the factor V Leiden mutation in cases and controls and the relative and attributable risk of venous thromboembolic disease ..
  18. VITAMIN K-DEPENDENT PROTEINS IN LIVER & ISOLATED CELLS
    Reidar Wallin; Fiscal Year: 1992
    ..Pulmonary macrophages produce tissue factor and factor V but appear to be devoid of carboxylase activity...
  19. DEFINITION OF FUNCTIONAL SITES ON COAGULATION FACTOR X
    WILLIAM CHURCH; Fiscal Year: 1993
    ..a supramolecular complex, termed prothrombinase, consisting of an enzyme (Factor X alpha), a protein cofactor (Factor V alpha), calcium ion, and a phospholipid surface...
  20. Processed Defining Megakaryocyte Endocytosis of Factor V
    PAULA TRACY; Fiscal Year: 2005
    ..the rate of thrombin generation, the physiologic effect being demonstrated by the severe hemorrhage observed in factor V deficiency...
  21. Discovery of antivirals against vaccinia and smallpox
    Robert Ricciardi; Fiscal Year: 2003
    ..The approach may help deliver the 'just-in-time' need for reagents to combat a smallpox bioterrorism threat. ..
  22. PROTHROMBIN ACTIVATION ON BLOOD MONONUCLEAR CELLS
    PAULA TRACY; Fiscal Year: 1990
    ..of monocytes/macrophages to assemble an endogenous prothrombinase through synthesis, secretion and activation of factor V, factor X and prothrombin...
  23. Lung Endothelium in Vaso-Occlusion
    Songwei Wu; Fiscal Year: 2006
    ....
  24. Factor Va regulation of prothrombinase activity
    JAMILA HIRBAWI; Fiscal Year: 2010
    The LONG-TERM goal of our research is to study and analyze the structure and function of the factor V molecule in order to understand key mechanisms involved in maintaining hemostasis in the human body...
  25. Genetic Modifiers in Children with Sickle Cell Anemia
    Russell Ware; Fiscal Year: 2005
    ..g. methylenetetrahydrofolate reductase, platelet glycoprotein IIIa, plasminogen activator inhibitor, prothrombin, Factor V, and Factor VII genes), brain injury repair (apolipoprotein E), bilirubin metabolism (the UDP-..
  26. NEWBORN SCREENING BY MULTIPLEX MOLECULAR ANALYSIS
    Edwin Naylor; Fiscal Year: 2002
    ..Alpha-1-Antitrypsin Deficiency Z allele (G9989a) and S allele (A7677T); 4). Hereditary Thrombophilia (Factor V Leiden G1691A, Prothrombin G20210A, Methylenetetrahydrofolate reductase C677T); 5)...
  27. COMMON VARIANTS IN CANDIDATE GENES AND PREMATURE MI RISK
    Stephen Schwartz; Fiscal Year: 2001
    ..Those data show that common polymorphisms in genes coding for two clotting factors, coagulation Factor V and coagulation Factor II, are risk factors for MI only among cigarette smokers in this sample...
  28. THROMBOSTATIN--A SELECTIVE ANTITHROMBIN
    Alvin Schmaier; Fiscal Year: 1999
    ..selectively block a-thrombin s ability to activate platelets without interfering with its ability to activate factor V and VIII and clot fibrinogen The specific aims of this proposal are as follows: 1...
  29. PROTHROMBINASE COMPLEX--STOPPED-FLOW KINETICS
    Sriram Krishnaswamy; Fiscal Year: 1991
    ..of prothrombinase assembly in the presence or absence of substrate will be conducted using factor Va and other factor V-derived peptides in order to better define regions of the cofactor that participate in the assembly and function ..
  30. THROMBOTIC, INFLAMMATORY & GENE MARKERS OF CVD IN WOMEN
    Paul Ridker; Fiscal Year: 2002
    ..They will also explore common genetic polymorphisms in the tPA, PAI-1, MTHFR, thrombomodulin, prothrombin, and factor V genes so that both inherited and environmental determinants of coronary thrombosis in women can simultaneously be ..
  31. ANTIPHOSPHOLIPID SYNDROME AND THE PROTEIN C PATHWAY
    Robert Roubey; Fiscal Year: 2000
    ..and purification of autoantibodies directed against protein C protein S, thrombin, thrombomodulin, factor V, and beta2GPI, and correlation of these autoantibodies with clinical manifestations of the a aPL syndrome; 2) the ..
  32. FACTOR V GENE DEFECTS IN THROMBOPHILIA
    WILLIAM KANE; Fiscal Year: 2001
    ..Activated protein C (APC) regulates the prothrombinese complex by inactivating factor V which serves as an essential protein cofactor...
  33. SECONDARY PREVENTION TRIAL OF VENOUS THROMBOSIS
    Paul Ridker; Fiscal Year: 2002
    ..low dose warfarin in the secondary prevention of venous thromboembolism (VTE) among patients with and without factor V Leiden mutation, a common inherited defect of hemostasis associated with increased risk of recurrent thrombus ..
  34. STRUCTURES OF COAGULATION COFACTORS
    STEPHEN EVERSE; Fiscal Year: 2003
    ..Crystallization trials will begin immediately using bovine and human factor V as well as recombinant factor VIIIa...
  35. HYPOXIC STRESS MECHANISMS IN RADIATION AND CHEMOTHERAPY
    Keith Laderoute; Fiscal Year: 2009
    ..An understanding of these MTF-1-dependent properties should aid in the development of novel prognostic and new modalities for neoplastic diseases. ..
  36. NEWBORN SCREENING BY MULTIPLEX MOLECULAR ANALYSIS
    Edwin Naylor; Fiscal Year: 1999
    ..sickle cell hemoglobinopathies S, C, and E alleles; alpha-1-Antitrypsin Deficiency S allele and Z allele; Factor V Leiden A1691G; and Hemochromatosis G845A...
  37. Molecular Diagnostic Assays for Genetic Disease
    Hashem Akhavan Tafti; Fiscal Year: 2001
    ..Applicant's Abstract): The objective of Phase 1 is to develop a sensitive test and kit for detection of the Factor V Leiden mutation in human blood using a new nucleic acid ligation-based technology invented at Lumigen...
  38. The roles of host hemostatic system in the pathogenicity of Group A Streptococcal
    Hongmin Sun; Fiscal Year: 2009
    ..In Specific Aim 2 the effects of variations in host hemostatic factors, such as Factor V and fibrinogen, on host susceptibility to GAS infection will be tested...
  39. Decidual-Endothelial Tissue Factor and IUGR
    Charles Lockwood; Fiscal Year: 2006
    ..These pathological obstetrical conditions are associated with acquired and inherited thrombophilias (e.g. Factor V Leiden)...
  40. INFLAMMATION MARKERS OVER TIME IN CARDIOVASCULAR DISEASE
    Russell Tracy; Fiscal Year: 2002
    ..IL-2 receptor; and 3) to measure in the same way markers of hemostatic and fibrinolytic activation, such as factor V/Va. Plasmin-a2 Anti-plasmin Complex, and D-dimer...
  41. Genetic Risk Factors for CVA in Children with Hb SS
    Abdullah Kutlar; Fiscal Year: 2004
    ..converting enzyme), ID (insertion/deletion) polymorphism, prothrombin 20210 G to A mutation, and mutations in the Factor V gene (Factor V Leiden, Rsa I polymorphisms; in exon 13 of the factor V gene known as R2 and R3 haplotypes, and ..
  42. ETIOLOGY OF STROKE IN CHILDREN WITH SICKLE CELL DISEASE
    Sherri Zimmerman; Fiscal Year: 2004
    ..with SCA for the presence of specific inherited thrombotic mutations, including mutations in the genes for Factor V, Factor VII, MTHFR, GPIIa, prothrombin and fibrinogen, using DNA analysis...
  43. DNA PROBES FOR FASTER DETECTION OF GENE DISORDERS
    Robert Henkens; Fiscal Year: 1999
    ..including: mutations in the breast cancer susceptibility gene BRCA1, a point mutation in the gene encoding Factor V, K-ras tumor DNA in blood plasma, and alternatively spliced mRNAs of the CD44 gene...
  44. Diagnosing DNA Mutations Using Mismatch Repair Enzymes
    LEONARD BAZAR; Fiscal Year: 2004
    ..FRET-MIDAS reagents for the detection of high-risk oncogenic human papilloma virus (HPV) and the Coagulation factor V Leiden mutation, which are associated with cervical cancer and thrombophilia, respectively...
  45. CELL MEDIATED HEMOSTASIS
    Harold Roberts; Fiscal Year: 2004
    ..These studies will use factor V molecules that have cleavage site mutations...
  46. BIOCHEMICAL STUDIES ON THE BLOOD COAGULATION PROCESS
    DONALD HANAHAN; Fiscal Year: 1980
    This project represents a continuing investigation of the biochemical nature of Factor V found in the plasma of man and cow. This labile protein plays an important role in the key reaction of conversion of prothrombin to thrombin...
  47. Molecular Diagnostic Assays for Genetic Diseases
    Hashem Akhavan Tafti; Fiscal Year: 2004
    Phase I of this proposal proved the feasibility of detection of the factor V Leiden mutation in human blood and correct determination of sample genotype as compared to a reference PCR method...
  48. SNP Detection with Unlabeled, Unamplified Target DNA
    WANDA WHITE; Fiscal Year: 2002
    ..detection could be used in medical clinics for identifying disease causing mutations, such as human coagulation factor V Leiden, and in laboratories to study allele frequency and construct genetic maps...
  49. AN ASSAY FOR GENETIC MUTATIONS CAUSING HYPERCOAGULATION
    SUDHAKAR MARLA; Fiscal Year: 2003
    ..developing a rapid test to identify simultaneously SNPs in three genes involved in hypercoagulation disorders [factor V (1691 G->A), prothrombrin (20210 G->A) and MTHFR (677 C->T)]...
  50. AN ASSAY FOR GENETIC MUTATIONS CAUSING HYPERCOAGULATION
    SUDHAKAR MARLA; Fiscal Year: 2000
    ..The goal of the proposed research is to develop a rapid, cost efficient, simultaneous assay for determination of Factor V Leiden gene, the G20210 prothrombin gene, and C677T MTHFR gene mutations...
  51. SELECTIVITY OF BACTERIAL TRANSCRIPTION
    MICHAEL CHAMBERLIN; Fiscal Year: 2000
    ....
  52. MOLECULAR BASIS OF COAGULATION PROTEASE SPECIFICITY
    Alireza R Rezaie; Fiscal Year: 2010
    ....
  53. Functional Domains of Coagulation Factor V
    Michael Kalafatis; Fiscal Year: 2007
    ..The procofactor, factor V, does not participate in prothrombinase...
  54. Association of Thrombophilia and Inflammation with Post-Thrombotic Syndrome
    Mary Cushman; Fiscal Year: 2008
    ..Resultant disability is estimated at 2 million lost workdays/year and medical costs as $300 million yearly. Findings here can form the basis for development of new therapies to treat, and moreover prevent,PTS. ..
  55. Role of Coagulation System Variability: Cardiac Surgery
    Brian Donahue; Fiscal Year: 2006
    ..The project will make extensive use of data in the Vanderbilt Cardiac Surgery Registry, a valuable database of genetic and clinical data presently being prospectively collected on elective adult cardiac surgery patients...
  56. Individual Propensity to Venous Thrombosis
    John Heit; Fiscal Year: 2009
    ..We will also investigate the joint effects of these susceptibility genes on VTE stratified on Factor V Leiden;and Aim 3: To determine if functional assays of circulating whole blood procoagulant activity associate ..
  57. Low-Cost Digital Imaging System for Evaluating Thrombosis
    Brian Cooley; Fiscal Year: 2008
    ..This imaging methodology may have a capacity for future adaptation to clinical thrombosis imaging. [unreadable] [unreadable] [unreadable]..
  58. Stabilized factor VIIIa as improved therapy for hemophilia A
    ANDREW GALE; Fiscal Year: 2008
    ..This treatment could improve the quality of life and overall health of the hemophilia A sufferers. ..
  59. Novel targets and agents to treat thrombotic disorders
    John Griffin; Fiscal Year: 2004
    ..abstract_text> ..
  60. Effect of Age on Vein Graft Neointimal Formation
    Brian Cooley; Fiscal Year: 2003
    ..Furthermore, the establishment of an age-associated model of vein grafting will permit age-specific evaluation of new therapeutic avenues for inhibiting neointimal formation. ..
  61. COAT-Platelets
    George Dale; Fiscal Year: 2005
    ..referred to a COAT-platelets (collagen and thrombin stimulated-platelets), express high levels of surface-bound factor V. This sub-population of platelets represents approximately 30 percent of the total and is most prominent among ..
  62. POSTDOCTORAL HEMATOLOGY RESEARCH TRAINING
    George Dale; Fiscal Year: 2003
    ..Francis Fdt. (OUHSC). All members of the faculty work closely together under Specialized Center of Research (SCOR) and Program Project (PP)...
  63. Outcomes of Perinatal Stroke
    Meredith Golomb; Fiscal Year: 2008
    ..During the last year of the study, she plans to apply for an R01 grant to set up a pilot clinical trial. [unreadable] [unreadable]..