bordetella virulence factors

Summary

Summary: A set of ADHESINS and TOXINS, BIOLOGICAL produced by BORDETELLA organisms that determine the pathogenesis of BORDETELLA INFECTIONS, such as WHOOPING COUGH. They include filamentous hemagglutinin; FIMBRIAE PROTEINS; pertactin; PERTUSSIS TOXIN; ADENYLATE CYCLASE TOXIN; dermonecrotic toxin; tracheal cytotoxin; Bordetella LIPOPOLYSACCHARIDES; and tracheal colonization factor.

Top Publications

  1. ncbi Phosphorelay control of virulence gene expression in Bordetella
    Peggy A Cotter
    Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106 9610, USA
    Trends Microbiol 11:367-73. 2003
  2. pmc Pathogen-specific T regulatory 1 cells induced in the respiratory tract by a bacterial molecule that stimulates interleukin 10 production by dendritic cells: a novel strategy for evasion of protective T helper type 1 responses by Bordetella pertussis
    Peter McGuirk
    Immune Regulation Research Group, Department of Biochemistry, Trinity College, Dublin 2, Ireland
    J Exp Med 195:221-31. 2002
  3. pmc Subtilisin-like autotransporter serves as maturation protease in a bacterial secretion pathway
    L Coutte
    INSERM U447, IBL, Institut Pasteur de Lille, 1 rue Calmette, 59019 Lille Cedex, France
    EMBO J 20:5040-8. 2001
  4. ncbi Regulation of type III secretion in Bordetella
    Seema Mattoo
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at UCLA, 10833 Le Conte Ave, Los Angeles, CA 90095 1747, USA
    Mol Microbiol 52:1201-14. 2004
  5. pmc Role of ADP-ribosyltransferase activity of pertussis toxin in toxin-adhesin redundancy with filamentous hemagglutinin during Bordetella pertussis infection
    S Alonso
    INSERM U447, IBL, Institut Pasteur de Lille, F-59019 Lille, France
    Infect Immun 69:6038-43. 2001
  6. pmc Common accessory genes for the Bordetella pertussis filamentous hemagglutinin and fimbriae share sequence similarities with the papC and papD gene families
    C Locht
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM CJF91 09, Lille, France
    EMBO J 11:3175-83. 1992
  7. pmc Nature of DNA binding and RNA polymerase interaction of the Bordetella pertussis BvgA transcriptional activator at the fha promoter
    P E Boucher
    Division of Bacterial Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    J Bacteriol 179:1755-63. 1997
  8. ncbi Tracheal colonization factor: a Bordetella pertussis secreted virulence determinant
    T M Finn
    Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892 0029, USA
    Mol Microbiol 16:625-34. 1995
  9. pmc Bordetella species are distinguished by patterns of substantial gene loss and host adaptation
    C A Cummings
    Departments of Microbiology and Immunology Medicine, Stanford University School of Medicine, Stanford, California 94305, USA
    J Bacteriol 186:1484-92. 2004
  10. ncbi Role of the polymorphic region 1 of the Bordetella pertussis protein pertactin in immunity
    A J King
    Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands
    Microbiology 147:2885-95. 2001

Research Grants

  1. Effect of Pertussis Toxin on Cough in Guinea Pig
    NICHOLAS CARBONETTI; Fiscal Year: 2005
  2. Pertussis Toxin Trafficking and Processing in Cells
    NICHOLAS CARBONETTI; Fiscal Year: 2001
  3. Role of Pertussis Toxin in Bordetella pertussis infection
    Nicholas H Carbonetti; Fiscal Year: 2010
  4. Pertussis Toxin Trafficking and Processing in Cells
    NICHOLAS CARBONETTI; Fiscal Year: 2002
  5. Role of Pertussis Toxin in Bordetella pertussis infection
    NICHOLAS CARBONETTI; Fiscal Year: 2009
  6. Role of Pertussis Toxin in Bordetella pertussis infection
    NICHOLAS CARBONETTI; Fiscal Year: 2009
  7. Pertussis Toxin Trafficking and Processing in Cells
    NICHOLAS CARBONETTI; Fiscal Year: 2003
  8. Role of Pertussis Toxin in Bordetella pertussis infection
    NICHOLAS CARBONETTI; Fiscal Year: 2007
  9. Pertussis Toxin Trafficking and Processing in Cells
    NICHOLAS CARBONETTI; Fiscal Year: 2004
  10. Role of Pertussis Toxin in Bordetella pertussis infection
    NICHOLAS CARBONETTI; Fiscal Year: 2008

Detail Information

Publications164 found, 100 shown here

  1. ncbi Phosphorelay control of virulence gene expression in Bordetella
    Peggy A Cotter
    Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106 9610, USA
    Trends Microbiol 11:367-73. 2003
  2. pmc Pathogen-specific T regulatory 1 cells induced in the respiratory tract by a bacterial molecule that stimulates interleukin 10 production by dendritic cells: a novel strategy for evasion of protective T helper type 1 responses by Bordetella pertussis
    Peter McGuirk
    Immune Regulation Research Group, Department of Biochemistry, Trinity College, Dublin 2, Ireland
    J Exp Med 195:221-31. 2002
    ..The induction of Tr1 cells after interaction of a pathogen-derived molecule with cells of the innate immune system represents a novel strategy exploited by an infectious pathogen to subvert protective immune responses in vivo...
  3. pmc Subtilisin-like autotransporter serves as maturation protease in a bacterial secretion pathway
    L Coutte
    INSERM U447, IBL, Institut Pasteur de Lille, 1 rue Calmette, 59019 Lille Cedex, France
    EMBO J 20:5040-8. 2001
    ..SphB1 is the first described subtilisin-like protein that serves as a specialized maturation protease in a secretion pathway of Gram-negative bacteria. This is reminiscent of pro-protein convertases of eukaryotic cells...
  4. ncbi Regulation of type III secretion in Bordetella
    Seema Mattoo
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at UCLA, 10833 Le Conte Ave, Los Angeles, CA 90095 1747, USA
    Mol Microbiol 52:1201-14. 2004
    ..The block in type III secretion is post-transcriptional in human-adapted strains, and signal recognition appears to be a point of divergence between subspecies...
  5. pmc Role of ADP-ribosyltransferase activity of pertussis toxin in toxin-adhesin redundancy with filamentous hemagglutinin during Bordetella pertussis infection
    S Alonso
    INSERM U447, IBL, Institut Pasteur de Lille, F-59019 Lille, France
    Infect Immun 69:6038-43. 2001
    ..This may lead to damage of the pulmonary epithelium, allowing the bacteria to colonize even in the absence of FHA...
  6. pmc Common accessory genes for the Bordetella pertussis filamentous hemagglutinin and fimbriae share sequence similarities with the papC and papD gene families
    C Locht
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM CJF91 09, Lille, France
    EMBO J 11:3175-83. 1992
    ..Deletion analyses suggest that fhaB and the downstream genes can be transcribed as a polycistronic operon, and primer extension analysis revealed the presence of a second promoter between fhaB and fhaD...
  7. pmc Nature of DNA binding and RNA polymerase interaction of the Bordetella pertussis BvgA transcriptional activator at the fha promoter
    P E Boucher
    Division of Bacterial Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    J Bacteriol 179:1755-63. 1997
    ....
  8. ncbi Tracheal colonization factor: a Bordetella pertussis secreted virulence determinant
    T M Finn
    Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892 0029, USA
    Mol Microbiol 16:625-34. 1995
    ..bronchiseptica and B. parapertussis, using a probe derived from tcfA, shows strong hybridization only to B. pertussis DNA. Thus, Tcf appears to be a unique virulence factor of B. pertussis...
  9. pmc Bordetella species are distinguished by patterns of substantial gene loss and host adaptation
    C A Cummings
    Departments of Microbiology and Immunology Medicine, Stanford University School of Medicine, Stanford, California 94305, USA
    J Bacteriol 186:1484-92. 2004
    ....
  10. ncbi Role of the polymorphic region 1 of the Bordetella pertussis protein pertactin in immunity
    A J King
    Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands
    Microbiology 147:2885-95. 2001
    ..An mAb directed against a linear conserved epitope conferred cross-immunity against isolates with distinct pertactin variants. The results indicate an important role of region 1 of pertactin in immunity...
  11. pmc Polymorphism in the Bordetella pertussis virulence factors P.69/pertactin and pertussis toxin in The Netherlands: temporal trends and evidence for vaccine-driven evolution
    F R Mooi
    Research Laboratory for Infectious Diseases, National Institute for Public Health and the Environment, Bilthoven, The Netherlands
    Infect Immun 66:670-5. 1998
    ..Our findings cast a new light on the reemergence of pertussis in highly vaccinated populations and may have major implications for the long-term efficacy of both WCVs and ACVs...
  12. pmc Polymorphisms of the fimbria fim3 gene of Bordetella pertussis strains isolated in Canada
    Raymond S W Tsang
    National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba, Canada R3E 3R2
    J Clin Microbiol 42:5364-7. 2004
    ..At least one of the novel mutations identified was found to involve a surface epitope that has been mapped by serum antibodies from infected or vaccinated subjects...
  13. pmc The crystal structure of filamentous hemagglutinin secretion domain and its implications for the two-partner secretion pathway
    Bernard Clantin
    Institut Fédératif de Recherche du Centre National de la Recherche Scientifique 3, Lille Cedex, France
    Proc Natl Acad Sci U S A 101:6194-9. 2004
    ..This structure is representative of the TPS domains found so far in >100 secreted proteins from pathogenic bacteria. It also provides a mechanistic insight into how protein folding may be linked to secretion in the TPS pathway...
  14. ncbi Temporal nucleotide changes in pertactin and pertussis toxin genes in Bordetella pertussis strains isolated from clinical cases in Poland
    A Gzyl
    Department of Sera and Vaccines Evaluation, National Institute of Hygiene, Chocimska Street 24, 00 791 Warsaw, Poland
    Vaccine 20:299-303. 2001
    ..pertussis in Poland and resulted in antigenic shift in both genes analyzed. Our findings may have implications for the composition of polish WCV and the currently licensed acellular pertussis vaccines...
  15. pmc Antigenic divergence suggested by correlation between antigenic variation and pulsed-field gel electrophoresis profiles of Bordetella pertussis isolates in Japan
    Atsuko Kodama
    Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama City, Tokyo 208 0011, Japan
    J Clin Microbiol 42:5453-7. 2004
    ..In contrast, the number of reported pertussis-like and pertussis cases decreased gradually from 1991 on, suggesting that the antigenic divergence did not affect the efficacy of pertussis vaccination in Japan...
  16. pmc Strain variation among Bordetella pertussis isolates from Québec and Alberta provinces of Canada from 1985 to 1994
    Mark S Peppler
    Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada
    J Clin Microbiol 41:3344-7. 2003
    ..This study provides a baseline for continued surveillance of prevalent and emerging strains of B. pertussis in Canada...
  17. pmc The Bvg virulence control system regulates biofilm formation in Bordetella bronchiseptica
    Yasuhiko Irie
    Department of Microbiology, University of Pennsylvania School of Medicine, 201C Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104 6084, USA
    J Bacteriol 186:5692-8. 2004
    ..However, expression of ACY inhibits biofilm formation, most likely via interactions with FHA. Therefore, the coordinated regulation of adhesins and ACY expression leads to maximal biofilm formation in the Bvgi phase in B. bronchiseptica...
  18. pmc Genotypic variation in the Bordetella pertussis virulence factors pertactin and pertussis toxin in historical and recent clinical isolates in the United Kingdom
    N K Fry
    Respiratory and Systemic Infection Laboratory, PHLS Central Public Health Laboratory, London, United Kingdom
    Infect Immun 69:5520-8. 2001
    ..The most striking difference in the United Kingdom isolates is that all 105 of the most recent circulating strains (from 1998 to 1999) are of a pertussis toxin type found in the United Kingdom WCV, i.e., ptxA(1)...
  19. pmc Proinflammatory and proapoptotic activities associated with Bordetella pertussis filamentous hemagglutinin
    T Abramson
    Department of Medicine, Stanford University School of Medicine, Stanford, California 94305, USA
    Infect Immun 69:2650-8. 2001
    ..pertussis-host interaction...
  20. ncbi Differences of circulating Bordetella pertussis population in Argentina from the strain used in vaccine production
    M Fingermann
    Instituto de Bioquimica y Biologia Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 1900 La Plata, Argentina
    Vaccine 24:3513-21. 2006
    ..bronchiseptica. The presence of this Bordetella species together with the observed differences between circulating B. pertussis and the strain used in vaccine production should be considered for the development of an improved vaccine...
  21. ncbi Invasion of human respiratory epithelial cells by Bordetella pertussis: possible role for a filamentous hemagglutinin Arg-Gly-Asp sequence and alpha5beta1 integrin
    Y Ishibashi
    Department of Immunobiology, Meiji Pharmaceutical University, Noshio, Kiyose, Tokyo 204 8588, Japan
    Microb Pathog 30:279-88. 2001
    ..pertussis invasion. Taken together, these findings suggest that B. pertussis FHA may promote invasion of human respiratory epithelial cells through the interaction of its RGD sequence with host cell alpha5beta1 integrin...
  22. pmc Bordetella filamentous hemagglutinin plays a critical role in immunomodulation, suggesting a mechanism for host specificity
    Carol S Inatsuka
    Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106 9610, USA
    Proc Natl Acad Sci U S A 102:18578-83. 2005
    ..These results indicate a critical role for FHA in B. bronchiseptica-mediated immunomodulation, and they suggest a role for FHA in host specificity...
  23. ncbi Role of nuclear factor-kappa B in the regulation of intercellular adhesion molecule 1 after infection of human bronchial epithelial cells by Bordetella pertussis
    Yoshio Ishibashi
    Department of Immunobiology, Meiji Pharmaceutical University, Noshio, Kiyose, Tokyo 204 8588, Japan
    Microb Pathog 35:169-77. 2003
    ....
  24. ncbi Bordetella dermonecrotic toxin undergoes proteolytic processing to be translocated from a dynamin-related endosome into the cytoplasm in an acidification-independent manner
    Takeshi Matsuzawa
    Department of Bacterial Toxinology, Research Institute for Microbial Diseases, Osaka University, Yamada oka 3 1, Suita, Osaka 565 0871, Japan
    J Biol Chem 279:2866-72. 2004
    ..DeltaB, liberated from the binding domain after the processing, begins to translocate the enzymatic domain into the cytoplasm...
  25. ncbi The Bordetella pertussis virulence factor P.69 pertactin retains its immunological properties after overproduction in Escherichia coli
    Marcel Hijnen
    Laboratory for Vaccine Preventable Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
    Protein Expr Purif 41:106-12. 2005
    ..Here, we describe the procedure to clone, express, and purify up to 0.1mg P.69 pertactin and its derivatives per 1 ml Escherichia coli culture...
  26. ncbi Sequence variation in pertussis S1 subunit toxin and pertussis genes in Bordetella pertussis strains used for the whole-cell pertussis vaccine produced in Poland since 1960: efficiency of the DTwP vaccine-induced immunity against currently circulating B.
    Anna Gzyl
    Department of Sera and Vaccines Evaluation, National Institute of Hygiene, Chocimska Street 24, 00 791 Warsaw, Poland
    Vaccine 22:2122-8. 2004
    ..pertussis vaccine strains, since isolates with gene variants different from those observed in vaccine strains were eliminated from the lungs of the immunized animals with lower efficiency...
  27. ncbi Bordetella dermonecrotic toxin exerting toxicity through activation of the small GTPase Rho
    Aya Fukui
    The Department of Bacterial Toxinology, Research Institute for Microbial Diseases, Osaka University, Yamada oka 3 1, Suita, Osaka 565 0871, Japan
    J Biochem 136:415-9. 2004
    ..Several lines of evidence show that the toxin undergoes proteolytic cleavage by furin or furin-like protease probably in the early endosome, and then escapes into the cytoplasm to reach the GTPase...
  28. pmc Polymorphism of Bordetella pertussis isolates circulating for the last 10 years in France, where a single effective whole-cell vaccine has been used for more than 30 years
    C Weber
    , , Institut Pasteur, Paris, France
    J Clin Microbiol 39:4396-403. 2001
    ..This observation suggests that there has been continuous evolution of the B. pertussis population...
  29. pmc Epitope structure of the Bordetella pertussis protein P.69 pertactin, a major vaccine component and protective antigen
    Marcel Hijnen
    Laboratory for Vaccine Preventable Diseases, National Institute of Public Health, Antonie van Leeuwenhoek Laan 1, P O Box 1, Bilthoven, The Netherlands
    Infect Immun 72:3716-23. 2004
    ..69 pertactin. This may explain why the variation in P.69 pertactin is so effective, despite the fact that it is limited to only two small segments of the molecule...
  30. pmc Temporal trends in circulating Bordetella pertussis strains in Australia
    M Poynten
    National Centre for Immunisation Research and Surveillance of Vaccine Preventable Diseases, Children s Hospital at Westmead, Sydney, NSW, Australia
    Epidemiol Infect 132:185-93. 2004
    ..Antigenic shift in B. pertussis may have contributed to the re-emergence of pertussis in Australia. Monitoring these trends will be important as acellular vaccines are introduced and changes are made to pertussis vaccine schedules...
  31. ncbi Bordetella pertussis, molecular pathogenesis under multiple aspects
    C Locht
    INSERM U447, Institut Pasteur de Lille, 1 rue du Prof Calmette, F 59019, Lille Cedex, France
    Curr Opin Microbiol 4:82-9. 2001
    ..They are regulated by iron, although in one case, the production of a siderophore receptor could be linked to the BvgAS regulon...
  32. pmc Role of Bordetella pertussis virulence factors in adherence to epithelial cell lines derived from the human respiratory tract
    B M van den Berg
    Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Infect Immun 67:1056-62. 1999
    ..pertussis to these cells. These data may imply that fimbriae play a role in infection of only the laryngeal mucosa, while FHA is the major factor in colonization of the entire respiratory tract...
  33. pmc Bordetella bronchiseptica dermonecrotizing toxin induces reorganization of actin stress fibers through deamidation of Gln-63 of the GTP-binding protein Rho
    Y Horiguchi
    Department of Bacterial Toxicology, Research Institute for Microbial Diseases, Osaka University, 3 1, Yamada oka, Suita, Osaka 565, Japan
    Proc Natl Acad Sci U S A 94:11623-6. 1997
    ..These results indicate that the toxin catalyzes deamidation of Gln-63 of Rho and renders it constitutively active, leading to formation of actin stress fibers...
  34. ncbi Structure of Bordetella pertussis virulence factor P.69 pertactin
    P Emsley
    Department of Chemistry, University of Glasgow, UK
    Nature 381:90-2. 1996
    ..The carboxy-terminal region of P.69 pertactin incorporates a (PQP)5 motif loop containing the major immunoprotective epitope...
  35. ncbi Secretion signal of the filamentous haemagglutinin, a model two-partner secretion substrate
    Hélène Hodak
    INSERM U629, 1 rue Calmette, F 59019 Lille cedex, France
    Mol Microbiol 61:368-82. 2006
    ..The N-terminal domain of FhaC is involved in the FHA-FhaC interaction, in agreement with its proposed function and periplasmic localization...
  36. ncbi Antibody responses to defined regions of the Bordetella pertussis virulence factor pertactin
    Marcel Hijnen
    Laboratory for Vaccine Preventable Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
    Scand J Infect Dis 40:94-104. 2008
    ..It also suggests that the amount of pertactin type-specific antibodies will not be very large and that the variation in pertactin probably will not constitute a problem in highly immune individuals...
  37. pmc Identification of a receptor-binding domain of Bordetella dermonecrotic toxin
    Takeshi Matsuzawa
    Department of Bacterial Toxinology, Research Institute for Microbial Diseases, Osaka University, Yamada oka 3 1, Suita, Osaka 565 0871, Japan
    Infect Immun 70:3427-32. 2002
    ..DNT(1-54) bound to none of the DNT-resistant cells, implying the presence of a cell surface receptor specific to DNT-sensitive cells...
  38. ncbi Molecular characterization of Bordetella bronchiseptica filamentous haemagglutinin and its secretion machinery
    F Jacob-Dubuisson
    INSERM U447, Institut de Biologie de Lille, France
    Microbiology 146:1211-21. 2000
    ..Characterization of FHA expression and function may provide clues as to the basis of host species tropism, tissue localization and receptor recognition...
  39. ncbi Cloning, nucleotide sequence and heterologous expression of the protective outer-membrane protein P.68 pertactin from Bordetella bronchiseptica
    J Li
    Wellcome Research Laboratories, Beckenham, Kent, UK
    J Gen Microbiol 138:1697-705. 1992
    ..The major differences between all three proteins occur in the number of repeats of the two families (Gly-Gly-Xaa-Xaa-Pro)n and (Pro-Gln-Pro)n of reiterated sequence motifs...
  40. ncbi Polymorphism in Bordetella pertussis pertactin and pertussis toxin virulence factors in the United States, 1935-1999
    P Cassiday
    Division for Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Infect Dis 182:1402-8. 2000
    ..Our results provide the basis for prospectively monitoring for changes among circulating B. pertussis that might have epidemiologic relevance...
  41. pmc Variation in the Bordetella pertussis virulence factors pertussis toxin and pertactin in vaccine strains and clinical isolates in Finland
    F R Mooi
    Research Laboratory for Infectious Diseases, National Institute for Public Health and the Environment, Bilthoven, The Netherlands
    Infect Immun 67:3133-4. 1999
    ..An interesting difference between the contemporary Finnish and Dutch B. pertussis populations was found in the frequencies of pertactin variants, possibly explaining the distinct epidemiology of pertussis in the two countries...
  42. pmc Purification and immunogenicity of a recombinant Bordetella pertussis S1S3FHA fusion protein expressed by Streptococcus gordonii
    Song F Lee
    Department of Applied Oral Sciences, Faculty of Dentistry, Dalhousie University, Halifax, Nova Scotia, Canada B3H 3J5
    Appl Environ Microbiol 68:4253-8. 2002
    ..gordonii and that the fusion protein is immunogenic. This multivalent fusion protein approach may be used in designing a new generation of acellular pertussis vaccines...
  43. pmc In vivo modifications of small GTPase Rac and Cdc42 by Bordetella dermonecrotic toxin
    Minako Masuda
    Department of Bacterial Toxinology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Infect Immun 70:998-1001. 2002
    ..MC3T3-E1 cells treated with DNT at high concentrations demonstrated extensive formations of lamellipodia and filopodia, which indicate the intracellular activation of Rac and Cdc42, respectively...
  44. pmc Characterization of a recombinant fragment that contains a carbohydrate recognition domain of the filamentous hemagglutinin
    D F Liu
    Wyeth Lederle Vaccines and Pediatrics, West Henrietta, New York 14586, USA Dai
    Infect Immun 65:3465-8. 1997
    ..pertussis to asialo-GM2 and to rabbit ciliated cells. These observations demonstrate that this fragment of FHA contains a cellular binding domain capable of eliciting functional antibodies...
  45. ncbi Functional characterization of the BvgAS two-component system of Bordetella holmesii
    Gabriele Gerlach
    Theodor Boveri Institut für Biowissenschaften, Lehrstuhl fur Mikrobiologie, Universitat Wurzburg, Am Hubland, D 97074 Wurzburg, Germany
    Microbiology 150:3715-29. 2004
    ..Furthermore, it is shown that, despite the high similarity of the BvgA proteins of B. pertussis and B. holmesii, promoter recognition by the response regulator proteins differs substantially in these organisms...
  46. pmc Role of the dermonecrotic toxin of Bordetella bronchiseptica in the pathogenesis of respiratory disease in swine
    Susan L Brockmeier
    Respiratory Diseases of Livestock Research Unit, USDA, Agricultural Research Service, National Animal Disease Center, Ames, Iowa 50010, USA
    Infect Immun 70:481-90. 2002
    ..Therefore, production of DNT by B. bronchiseptica is necessary to produce the lesions of turbinate atrophy and bronchopneumonia in pigs infected with this organism...
  47. ncbi The C-type lectin fold as an evolutionary solution for massive sequence variation
    Stephen A McMahon
    Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California 92093, USA
    Nat Struct Mol Biol 12:886-92. 2005
    ..Mtd variants are biased toward the receptor pertactin, and there is evidence that the CLec fold is used broadly for sequence variation by related retroelements...
  48. ncbi Epidemiological typing of Bordetella pertussis isolates: recommendations for a standard methodology
    F R Mooi
    Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
    Eur J Clin Microbiol Infect Dis 19:174-81. 2000
    ..Comparisons may reveal whether the epidemiological differences observed between countries are due for instance to different Bordetella pertussis populations or different vaccines used...
  49. ncbi Crucial role of antibodies to pertactin in Bordetella pertussis immunity
    Sandra M M Hellwig
    Immunotherapy Laboratory, Department of Immunology, University Medical Center, Utrecht, The Netherlands
    J Infect Dis 188:738-42. 2003
    ..pertussis phagocytosis. These data are consistent with field studies showing that levels of antibodies to pertactin correlate with protection...
  50. ncbi Antigenic variants in Bordetella pertussis strains isolated from vaccinated and unvaccinated children
    P Mastrantonio
    Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy
    Microbiology 145:2069-75. 1999
    ..Prn2 strains showed a greater variety of fingerprint types compared to Prn3, suggesting that Prn3 has emerged more recently. The results are discussed in the light of vaccine-driven evolution...
  51. ncbi Chemoattractant receptors activate distinct pathways for chemotaxis and secretion. Role of G-protein usage
    B Haribabu
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 274:37087-92. 1999
    ..Chemotaxis, however, had an absolute requirement for a G(i)-mediated pathway...
  52. ncbi Novel topological features of FhaC, the outer membrane transporter involved in the secretion of the Bordetella pertussis filamentous hemagglutinin
    S Guédin
    INSERM U447, IBL, Institut Pasteur de Lille, 1 rue Calmette, 59019 Lille Cedex, France
    J Biol Chem 275:30202-10. 2000
    ..The surface exposure of the N terminus and the presence of an odd number of beta-strands are novel features for beta-barrel outer membrane proteins...
  53. pmc Pertussis toxin inhibits neutrophil recruitment to delay antibody-mediated clearance of Bordetella pertussis
    Girish S Kirimanjeswara
    Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Clin Invest 115:3594-601. 2005
    ..Together these results indicate that PTx inhibits neutrophil recruitment, which consequently allows B. pertussis to avoid rapid antibody-mediated clearance and therefore successfully infect immune hosts...
  54. pmc Rapid identification of Bordetella pertussis pertactin gene variants using LightCycler real-time polymerase chain reaction combined with melting curve analysis and gel electrophoresis
    J Makinen
    Finnish Pertussis Reference Laboratory, National Public Health Institute, Department in Turku, Finland
    Emerg Infect Dis 7:952-8. 2001
    ..This method correctly identified the prn type of all tested 41 clinical isolates and two Finnish vaccine strains. The method is simple and reliable and provides an alternative for sequencing in pertussis research...
  55. ncbi Genetic characterization of Bordetella pertussis filamentous haemagglutinin: a protein processed from an unusually large precursor
    M Domenighini
    Sclavo Research Centre, Siena, Italy
    Mol Microbiol 4:787-800. 1990
    ..These data, as well as N-terminal amino acid sequencing of the major polypeptide species, suggest a scheme for the proteolytic processing of an FHA precursor polypeptide...
  56. ncbi Molecular aspects of Bordetella pertussis pathogenesis
    C Locht
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM, Institut Pasteur de Lille, France
    Int Microbiol 2:137-44. 1999
    ..pertussis strains to be used as recombinant live bacterial vaccine vectors for homologous and heterologous protection...
  57. pmc Differential regulation of Bvg-activated virulence factors plays a role in Bordetella pertussis pathogenicity
    S M Kinnear
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Infect Immun 69:1983-93. 2001
    ..These data suggest a role for differential regulation of bvg-activated genes, and therefore for the BvgAS regulatory system, in the pathogenicity of B. pertussis...
  58. ncbi The new strategy for allele identification of the genes coding for pertussis toxin subunit S1 (ptx S1) and pertactin (prn) in Bordetella pertussis
    Janusz Fiett
    National Institute of Public Health, ul Chelmska 30 34, 00 725 Warsaw, Poland
    J Microbiol Methods 55:651-66. 2003
    ..pertussis strains isolated from the 1960s to 2001. Similarly to findings from other countries and to earlier Polish data, the tendency to change the vaccine types of PT S1 and Prn by the antigenically different ones was observed...
  59. pmc Simple Algorithm for Identification of Bordetella pertussis Pertactin Gene Variants
    Gaëtan Muyldermans
    Belgian Reference Laboratory for Pertussis, Department of Microbiology, Academisch Ziekenhuis Vrije Universiteit Brussel, Brussels, Belgium
    J Clin Microbiol 42:1614-9. 2004
    ..The typing of 231 clinical strains of B. pertussis routinely isolated in Belgium showed that this algorithm was adequate to identify less-frequent prn types like prn9 and prn11...
  60. pmc Rapid typing of Bordetella pertussis pertussis toxin gene variants by LightCycler real-time PCR and fluorescence resonance energy transfer hybridization probe melting curve analysis
    Johanna Mäkinen
    National Public Health Institute, Department in Turku, Finland
    J Clin Microbiol 40:2213-6. 2002
    ..The assay correctly identified the ptxS1 alleles of all strains tested, comprising 57 Finnish clinical isolates and 2 vaccine strains. The method is simple, reliable, and suitable for large-scale screening of B. pertussis strains...
  61. ncbi Bordetella pertussis PCR: simultaneous targeting of signature sequences
    Xuan Qin
    Microbiology Laboratory, Department of Laboratories and Pathology, CH 37, Children s Hospital and Regional Medical Center, Seattle, Washington 98105, USA
    Diagn Microbiol Infect Dis 43:269-75. 2002
    ..Simultaneous detection of multiple chromosomal regions may identify false-positive and -negative results due to analytical variations or potential sequence polymorphism, and uncover a wider range of pathogenic strains...
  62. ncbi Changes in the Dutch Bordetella pertussis population in the first 20 years after the introduction of whole-cell vaccines
    Inge H M Van Loo
    Laboratory for Infectious Diseases Research LIO, National Institute of Public Health and Environment, 3720 BA Bilthoven, The Netherlands
    Microbiology 148:2011-8. 2002
    ..This adaptation had very little, if any, effect on mortality, however...
  63. pmc Investigation of role of nitric oxide in protection from Bordetella pertussis respiratory challenge
    C Canthaboo
    Division of Bacteriology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, United Kingdom
    Infect Immun 70:679-84. 2002
    ..iNOS-deficient mice also developed a less effective protective response than wild-type mice after the same immunization with WCV. This suggests that NO plays an important role in effecting protection against B. pertussis challenge...
  64. ncbi Determination of pertactin IgG antibodies for the diagnosis of pertussis
    B Trollfors
    Department of Pediatrics, Sahlgrenska University Hospital East, Goteborg University, Goteborg, Sweden
    Clin Microbiol Infect 9:585-9. 2003
    ....
  65. ncbi Bordetella pertussis infection of human respiratory epithelial cells up-regulates intercellular adhesion molecule-1 expression: role of filamentous hemagglutinin and pertussis toxin
    Yoshio Ishibashi
    Department of Immunobiology, Meiji Pharmaceutical University, Noshio, Kiyose, Tokyo 204 8588, Japan
    Microb Pathog 33:115-25. 2002
    ..These findings demonstrate that B. pertussis FHA up-regulates ICAM-1 expression on respiratory epithelial cells through interaction of its RGD site with host cell VLA-5 integrin, and that PT impairs this response...
  66. ncbi Toxicity and potency evaluation of pertussis vaccines
    Michael J Corbel
    Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, EN6 3QG, UK
    Expert Rev Vaccines 3:89-101. 2004
    ..Genome sequence data and the use of gene microarrays to screen responses triggered by vaccine components may also provide leads to improved methods for assessing both toxicity and immunogenicity...
  67. ncbi Bordetella pertussis infection in 2-month-old infants promotes type 1 T cell responses
    Francoise Mascart
    Laboratory of Immunology, Hopital Erasme, Universite Libre de Bruxelles, Brussels, Belgium
    J Immunol 170:1504-9. 2003
    ..We conclude that the immune system of 2-mo-old infants is developmentally mature enough to develop Th1 responses in vivo upon infection by B. pertussis or vaccination with whole-cell pertussis vaccines...
  68. pmc Role of adhesin release for mucosal colonization by a bacterial pathogen
    Loic Coutte
    INSERM U447, Institut National de Sante et la Recherche Medical Institut de Biologie de Lille, Institut Pasteur de Lille, 1 rue Calmette, 59019 Lille Cedex, France
    J Exp Med 197:735-42. 2003
    ..These results indicate that the release of FHA is important for colonization, as it may facilitate the dispersal of bacteria from microcolonies and the binding to new sites in the respiratory tract...
  69. ncbi Immunogenicity of a three-component acellular pertussis vaccine administered at birth
    Cesare Belloni
    Division of Neonatology and Neonatal Intensive Care, IRCCS Policlinico San Matteo Pavia, Italy
    Pediatrics 111:1042-5. 2003
    ..Furthermore, we investigated the influence of maternal antibodies on aP vaccine response...
  70. ncbi Dynamics of transplacental transmission of pertussis antibodies in premature and full-term infants
    Masahiro Nomura
    Department of Pediatrics, School of Medicine, Tokai University, Isehara City, Kanagawa, Japan
    Tokai J Exp Clin Med 28:21-6. 2003
  71. ncbi Prevalence of pertussis antibodies in maternal delivery, cord, and infant serum
    C Mary Healy
    Section of Infectious Diseases, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA
    J Infect Dis 190:335-40. 2004
    ..Passively acquired maternal antibodies protect infants from many pathogens. With increasing reports of infant pertussis, we evaluated pertussis antibodies in maternal-infant paired sera from 1999-2000...
  72. ncbi Long-term immunogenicity of a single dose of acellular pertussis vaccine in paediatric health-care workers
    Martina Littmann
    Landesamt für Gesundheit und Soziales Mecklenburg Vorpommern, Rostock, Germany
    Vaccine 26:2344-9. 2008
    ..Monitor the long-term immunogenicity of a single dose of acellular pertussis vaccine in health-care workers...
  73. ncbi [Application of DNA microcarriers for evaluation of mice splenocytes genes expression after immunization with pertussis vaccines and challenged with different B. pertussis strains]
    Anna Gzyl
    Zakład Badania Surowic i Szczepionek PZH w Warszawie
    Med Dosw Mikrobiol 59:149-59. 2007
    ..pertussis strains variant's for statistical evaluation of functional differences detected in presented study...
  74. ncbi [Evaluation of whole-cell and acellular pertussis vaccines effectiveness in clearance of experimental B. pertussis infection in mice]
    Anna Gzyl
    Zakład Badania Surowic i Szczepionek PZH w Warszawie
    Med Dosw Mikrobiol 59:123-35. 2007
    ..The study indicated for lowered efficiency of whole-cell and acellular pertussis vaccines in elimination of mutated strains of B. pertussis from animal lung tissue in comparison with strains currently used for vaccine production...
  75. pmc Construction and characterization of single-chain variable fragment antibodies directed against the Bordetella pertussis surface adhesins filamentous hemagglutinin and pertactin
    Ahmad H Hussein
    Department of Applied Oral Sciences, Faculty of Dentistry, Dalhousie University Halifax, Nova Scotia B3H 3J5, Canada
    Infect Immun 75:5476-82. 2007
    ..BALB/c mice who received recombinant phage-treated B. pertussis had reduced bacterial counts in the nasal cavity, trachea, and lungs compared to the control groups...
  76. pmc Pulsed-field gel electrophoresis, pertactin, pertussis toxin S1 subunit polymorphisms, and surfaceome analysis of vaccine and clinical Bordetella pertussis strains
    Daniela Bottero
    Instituto de Bioquimica y Biologia Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 1900, La Plata, Republica Argentina
    Clin Vaccine Immunol 14:1490-8. 2007
    ..Some of them have been proposed as novel vaccine candidate proteins for other pathogens. Overall, the global strategy described here is presented as a good tool for the development of next-generation acellular vaccines...
  77. ncbi [The risk of pertussis and diphtheria infections among pediatric healthcare workers in Japan]
    Kazunari Kamachi
    National Institute of Infectious Diseases Department of Bacterial Pathogenesis and Infection Control
    Kansenshogaku Zasshi 81:155-61. 2007
    ..For pertussis and diphtheria infection control in pediatric hospitals, it is important for healthcare workers to be aware of their own protection levels against these diseases...
  78. ncbi Long-term humoral and cell-mediated immunity after acellular pertussis vaccination compares favourably with whole-cell vaccines 6 years after booster vaccination in the second year of life
    N Guiso
    Unité de Prévention et Thérapies Moléculaires des Maladies Humaines FRE CNRS 2849, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Vaccine 25:1390-7. 2007
    ..At year 6, CMI responses continued to be present and were higher in Pa-vaccinated than Pw-vaccinated subjects. Long-term protection with Pa vaccines can be expected to be at least as good as that provided by efficacious Pw vaccines...
  79. ncbi Adjuvant effects of adenylate cyclase toxin of Bordetella pertussis after intranasal immunisation of mice
    Barbara Orr
    Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow G12 8TA, UK
    Vaccine 25:64-71. 2007
    ..Co-administration of CyaA or CyaA* with pertactin (Prn) significantly increased protection (P<0.05) against challenge compared to that obtained with Prn alone. These effects were particularly apparent with CyaA* as the adjuvant...
  80. pmc Immunogenicity and protective efficacy of a recombinant filamentous haemagglutinin from Bordetella pertussis
    J B Knight
    Department of Microbiology and Immunology, Faculty of Medicine, Dalhousie University and the IWK Health Centre, Halifax, NS, Canada
    Clin Exp Immunol 144:543-51. 2006
    ..pertussis in BALB/c mice. These data indicate that the type I domain of FHA is a protective antigen in mice and may serve as a candidate for inclusion in new acellular pertussis vaccines...
  81. pmc Bordetella pertussis, Finland and France
    Valerie Caro
    Institut Pasteur, Unité PTMMH, FRE CNRS 2849, National Center of Reference of Whooping Cough and Other Bordetelloses, Paris, France
    Emerg Infect Dis 12:987-9. 2006
    ..Isolates from both countries were similar genetically but varied temporally...
  82. pmc Analysis of Bordetella pertussis pertactin and pertussis toxin types from Queensland, Australia, 1999-2003
    Shane Byrne
    Public Health Microbiology, Queensland Health Scientific Services, Brisbane, Australia
    BMC Infect Dis 6:53. 2006
    ..This study was conducted to survey the pertactin and pertussis toxin subunit 1 types from B. pertussis isolates in Queensland, Australia following the introduction of acellular vaccines...
  83. pmc Evaluation of a tetraplex microsphere assay for Bordetella pertussis antibodies
    Harry E Prince
    Focus Diagnostics, 5785 Corporate Avenue, Cypress, CA 90630, USA
    Clin Vaccine Immunol 13:266-70. 2006
    ..These findings show that antibodies to B. pertussis antigens can be measured easily and accurately using a tetraplex microsphere system...
  84. ncbi Genetic and antigenic analysis of Bordetella pertussis isolates recovered from clinical cases in Ontario, Canada, before and after the introduction of the acellular pertussis vaccine
    Raymond S W Tsang
    Vaccine Preventable Bacterial Disease Division, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada
    Can J Microbiol 51:887-92. 2005
    ..Further surveillance with clinical data and isolates collected periodically will be required to ensure that any genetic divergence that could affect vaccine efficacy will not be occurring...
  85. pmc Age-specific long-term course of IgG antibodies to pertussis toxin after symptomatic infection with Bordetella pertussis
    F G A Versteegh
    Department of Pediatrics, Groene Hart Hospital, Gouda, The Netherlands
    Epidemiol Infect 133:737-48. 2005
    ..The IgG-PT cut-off of 100 U/ml for serodiagnosis of pertussis appeared valid in all age groups. A decline of IgG-PT to < 10 U/ml was associated with increased risk of re-infection with B. pertussis...
  86. pmc Shifts of Bordetella pertussis variants in Sweden from 1970 to 2003, during three periods marked by different vaccination programs
    Hans O Hallander
    Department of Immunology and Vaccine Research, Swedish Institute for Infectious Disease Control SMI, S 171 82 Solna, Sweden
    J Clin Microbiol 43:2856-65. 2005
    ..e., the vaccination program so far has been effective against whooping cough, and there seems to be no impact on the effectiveness of the vaccination program from the bacterial polymorphism...
  87. ncbi Adjuvanticity of native and detoxified adenylate cyclase toxin of Bordetella pertussis towards co-administered antigens
    Julia Macdonald-Fyall
    Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK
    Vaccine 22:4270-81. 2004
    ..pertussis, but a statistically significant increase in protection was seen after intranasal challenge with B. parapertussis...
  88. doi Development and validation of a multiplex immunoassay for the simultaneous determination of serum antibodies to Bordetella pertussis, diphtheria and tetanus
    Pieter G M van Gageldonk
    Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
    J Immunol Methods 335:79-89. 2008
    ..The pentaplex MIA shares features of the ELISA with the additional advantages of high sample throughput and small sample volumes and antigen required...
  89. ncbi Stability of antibodies to Bordetella antigens in German adults
    G Saemann-Ischenko
    , Klinikum Krefeld, Germany
    Eur J Clin Microbiol Infect Dis 20:850-3. 2001
    ..2 and 1.0 per 100 person-years in the population studied...
  90. ncbi International collaborative study: evaluation of proposed International Reference Reagent of pertussis antiserum (mouse) 97/642
    R Gaines Das
    Informatics Laboratory, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, EN6 3QG, UK
    Biologicals 29:137-48. 2001
    ..These recommendations were agreed by the Expert Committee on Biological Standardization of the World Health Organization...
  91. ncbi Protection against Bordetella pertussis infection following parenteral or oral immunization with antigens entrapped in biodegradable particles: effect of formulation and route of immunization on induction of Th1 and Th2 cells
    M A Conway
    Infection and Immunity Group, Institute of Immunology, Department of Biology, National University of Ireland, Co. Kildare, Maynooth, Ireland
    Vaccine 19:1940-50. 2001
    ..p.) or intramuscular (i.m.) route. In contrast, nanoparticle formulations favoured the induction of Th2 cells...
  92. ncbi Expression, activity and cytotoxicity of pertussis toxin S1 subunit in transfected mammalian cells
    M G Castro
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201, USA
    Cell Microbiol 3:45-54. 2001
    ....
  93. ncbi Distribution of pertussis antibodies among different age groups in Japan
    Toshifumi Konda
    Department of Bacterial and Blood Products, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama shi, Tokyo 208 0011, Japan
    Vaccine 20:1711-7. 2002
    ..Since intermittent circulation of B. pertussis remains among the Japanese population, complete vaccination with aP vaccine for all infants should be highly recommended to prevent pertussis...
  94. pmc Pertussis toxin and lipopolysaccharide influence phagocytosis of Bordetella pertussis by human monocytes
    L M Schaeffer
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267, USA
    Infect Immun 69:7635-41. 2001
    ..These results suggest that pertussis toxin plays a role in the evasion of monocyte phagocytosis and that these cells represent a potential mediator of the clearance of B. pertussis infection...
  95. ncbi Intranasal murine model of Bordetella pertussis infection: II. Sequence variation and protection induced by a tricomponent acellular vaccine
    C Boursaux-Eude
    Laboratoire des Bordetella, Institut Pasteur, Paris, France
    Vaccine 17:2651-60. 1999
    ..pertussis variants...
  96. ncbi [Cellular and acellular anti-pertussis vaccines]
    C Locht
    INSERM U447, Institut Pasteur de Lille, 1 rue du Prof. Calmette, F59019 Lille Cedex
    Ann Pharm Fr 59:198-205. 2001
    ..In addition, the construction of recombinant attenuated strains would perhaps simultaneously protect against whooping cough and other infectious or parasitic diseases...
  97. ncbi Efficacy of pertussis components in an acellular vaccine, as assessed in a murine model of respiratory infection and a murine intracerebral challenge model
    Mineo Watanabe
    Research Center for Biologicals, The Kitasato Institute, 6 111 Arai, Kitamoto, 364 0026, Saitama, Japan
    Vaccine 20:1429-34. 2002
    ..Ratios of FHA:PT of 1:11 or 2:10 (w/w) might be suitable for future formulations...
  98. ncbi A proposed mechanism of ADP-ribosylation catalyzed by the pertussis toxin S1 subunit
    C Locht
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM CJF 9109, Institut Pasteur de Lille, France
    Biochimie 77:333-40. 1995
    ..His-35 could increase the nucleophilicity of the cysteine in the G protein or the water molecule to attack the weakened N-glycosidic bond of NAD+ and yield the products of the reaction...
  99. ncbi Characteristics and potency of an acellular pertussis vaccine composed of pertussis toxin, filamentous hemagglutinin, and pertactin
    G C Sheu
    Vaccine Development Center, Taipei, Taiwan, ROC
    J Microbiol Immunol Infect 34:243-51. 2001
    ..More importantly, in intracerebral challenge assay in mouse, this vaccine also provided a better protection than DTaP(J)...
  100. pmc Effect of mutations causing overexpression of RNA polymerase alpha subunit on regulation of virulence factors in Bordetella pertussis
    N H Carbonetti
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201
    J Bacteriol 176:7267-73. 1994
    ..pertussis, which caused the same phenotype as the mutants showed. Conclusions in regard to the identity of the transcription activator of the toxin genes are discussed...
  101. pmc Immunogenicity and protective efficacy of neonatal vaccination against Bordetella pertussis in a murine model: evidence for early control of pertussis
    Caroline Roduit
    World Health Organization Collaborating Center for Vaccinology and Neonatal Immunology, Department of Pathology and Pediatrics, University of Geneva, Geneva, Switzerland
    Infect Immun 70:3521-8. 2002
    ..pertussis challenge. Thus, neonatal priming with antipertussis vaccines should be considered to reduce the window of vulnerability to pertussis at the time of its greatest severity...

Research Grants138 found, 100 shown here

  1. Effect of Pertussis Toxin on Cough in Guinea Pig
    NICHOLAS CARBONETTI; Fiscal Year: 2005
    ..abstract_text> ..
  2. Pertussis Toxin Trafficking and Processing in Cells
    NICHOLAS CARBONETTI; Fiscal Year: 2001
    ..Therefore the specific aims of this proposal are to investigate the trafficking, processing and transcytosis of PT in mammalian cells and the key features of this toxin that mediate these events. ..
  3. Role of Pertussis Toxin in Bordetella pertussis infection
    Nicholas H Carbonetti; Fiscal Year: 2010
    ..pertussis infection will increase our understanding of the pathogenic mechanisms of this organism, and may lead to novel therapeutic approaches to combat pertussis infection and disease. ..
  4. Pertussis Toxin Trafficking and Processing in Cells
    NICHOLAS CARBONETTI; Fiscal Year: 2002
    ..Therefore the specific aims of this proposal are to investigate the trafficking, processing and transcytosis of PT in mammalian cells and the key features of this toxin that mediate these events. ..
  5. Role of Pertussis Toxin in Bordetella pertussis infection
    NICHOLAS CARBONETTI; Fiscal Year: 2009
    ..pertussis infection will increase our understanding of the pathogenic mechanisms of this organism, and may lead to novel therapeutic approaches to combat pertussis infection and disease. ..
  6. Role of Pertussis Toxin in Bordetella pertussis infection
    NICHOLAS CARBONETTI; Fiscal Year: 2009
    ..pertussis infection will increase our understanding of the pathogenic mechanisms of this organism, and may lead to novel therapeutic approaches to combat pertussis infection and disease. ..
  7. Pertussis Toxin Trafficking and Processing in Cells
    NICHOLAS CARBONETTI; Fiscal Year: 2003
    ..Therefore the specific aims of this proposal are to investigate the trafficking, processing and transcytosis of PT in mammalian cells and the key features of this toxin that mediate these events. ..
  8. Role of Pertussis Toxin in Bordetella pertussis infection
    NICHOLAS CARBONETTI; Fiscal Year: 2007
    ..Elucidation of the specific roles of PT during B. pertussis infection will increase our understanding of the pathogenic mechanisms of this organism, and may lead to novel therapeutic approaches to combat pertussis infection and disease. ..
  9. Pertussis Toxin Trafficking and Processing in Cells
    NICHOLAS CARBONETTI; Fiscal Year: 2004
    ..Therefore the specific aims of this proposal are to investigate the trafficking, processing and transcytosis of PT in mammalian cells and the key features of this toxin that mediate these events. ..
  10. Role of Pertussis Toxin in Bordetella pertussis infection
    NICHOLAS CARBONETTI; Fiscal Year: 2008
    ..pertussis infection will increase our understanding of the pathogenic mechanisms of this organism, and may lead to novel therapeutic approaches to combat pertussis infection and disease. ..
  11. Role of Pertussis Toxin in Bordetella pertussis infection
    NICHOLAS CARBONETTI; Fiscal Year: 2006
    ..Elucidation of the specific roles of PT during B. pertussis infection will increase our understanding of the pathogenic mechanisms of this organism, and may lead to novel therapeutic approaches to combat pertussis infection and disease. ..
  12. Pertussis Toxin Trafficking and Processing in Cells
    NICHOLAS CARBONETTI; Fiscal Year: 2005
    ..Therefore the specific aims of this proposal are to investigate the trafficking, processing and transcytosis of PT in mammalian cells and the key features of this toxin that mediate these events. ..
  13. Early Immune Events in Childhood Asthma
    Lisa Miller; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  14. Bordetella cyclase toxin: effects on leukocytes and respiratory epithelial cells
    Erik Hewlett; Fiscal Year: 2009
    ..The objective is to apply the information on AC toxin and on other bordetellae virulence factors, to a more focused study of how AC toxin contributes to infections and diseases caused by this genus. ..
  15. BORDETELLA CYCLASE-STRUCTURE AND BIOLOGICAL ACTIVITIES
    Erik Hewlett; Fiscal Year: 2005
    ....
  16. Role of Eotaxin-3/CCL26 in Allergic Asthma
    Lisa Miller; Fiscal Year: 2007
    ..The identification of chemokines that promote allergic asthma is an important step in the future development of therapeutics to target specific molecules within the airways. [unreadable] [unreadable] [unreadable]..
  17. Role of Epithelium in Airway Immunity
    Lisa Miller; Fiscal Year: 2009
    ..End of Abstract) ..
  18. BORDETELLA CYCLASE-STRUCTURE AND BIOLOGICAL ACTIVITIES
    Erik Hewlett; Fiscal Year: 2002
    ....
  19. Bordetella cyclase toxin: effects on leukocytes and respiratory epithelial cells
    Erik Hewlett; Fiscal Year: 2006
    ..The objective is to apply the information on AC toxin and on other bordetellae virulence factors, to a more focused study of how AC toxin contributes to infections and diseases caused by this genus. ..
  20. Seventh International Symposium on Pertussis
    Erik Hewlett; Fiscal Year: 2002
    ..This meeting will lead to a greater scientific understanding of the biology of this organism, and the promise for developing a more effective means to control this disease. ..
  21. Early Immune Events in Childhood Asthma
    Lisa Miller; Fiscal Year: 2009
    ..abstract_text> ..
  22. BORDETELLA CYCLASE-STRUCTURE AND BIOLOGICAL ACTIVITIES
    Erik Hewlett; Fiscal Year: 2003
    ....
  23. Bordetella cyclase toxin: effects on leukocytes and respiratory epithelial cells
    Erik Hewlett; Fiscal Year: 2008
    ..The objective is to apply the information on AC toxin and on other bordetellae virulence factors, to a more focused study of how AC toxin contributes to infections and diseases caused by this genus. ..
  24. 8th International Symposium - Saga of the Genus Bordetella, 1906-2006
    Erik Hewlett; Fiscal Year: 2006
    ..unreadable] The bordetella community is an especially open and collaborative[unreadable] collection of scientists and it is important to assure and support[unreadable] attendance of trainees at meetings such as this. ..
  25. BORDETELLA CYCLASE-STRUCTURE AND BIOLOGICAL ACTIVITIES
    Erik Hewlett; Fiscal Year: 2004
    ....
  26. Bordetella cyclase toxin: effects on leukocytes and respiratory epithelial cells
    Erik Hewlett; Fiscal Year: 2007
    ..The objective is to apply the information on AC toxin and on other bordetellae virulence factors, to a more focused study of how AC toxin contributes to infections and diseases caused by this genus. ..
  27. Early Immune Events in Childhood Asthma
    Lisa Miller; Fiscal Year: 2008
    ..abstract_text> ..
  28. Role of Eotaxin-3/CCL26 in Allergic Asthma
    Lisa Miller; Fiscal Year: 2008
    ..The identification of chemokines that promote allergic asthma is an important step in the future development of therapeutics to target specific molecules within the airways. [unreadable] [unreadable] [unreadable]..
  29. Early Immune Events in Childhood Asthma
    Lisa Miller; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  30. BORDETELLA CYCLASE: STRUCTURE AND BIOLOGICAL ACTIVITY
    Erik Hewlett; Fiscal Year: 1993
    ....
  31. BORDETELLA CYCLASE: STRUCTURE AND BIOLOGICAL ACTIVITY
    Erik Hewlett; Fiscal Year: 1991
    ..The work proposed in this application and ongoing at present will provide information of use in vaccine development and of importance to the understanding of bacterial toxins and their roles in pathogenesis of bacterial disease...
  32. Bordetella cyclase toxin: effects on leukocytes and respiratory epithelial cells
    Erik L Hewlett; Fiscal Year: 2010
    ..The objective is to apply the information on AC toxin and on other bordetellae virulence factors, to a more focused study of how AC toxin contributes to infections and diseases caused by this genus. ..
  33. BORDETELLA CYCLASE: STRUCTURE AND BIOLOGICAL ACTIVITY
    Erik Hewlett; Fiscal Year: 1990
    ..The work proposed in this application and ongoing at present will provide information of use in vaccine development and of importance to the understanding of bacterial toxins and their roles in pathogenesis of bacterial disease...
  34. Bordetella cyclase toxin: effects on leukocytes and respiratory epithelial cells
    Erik Hewlett; Fiscal Year: 2009
    ..The objective is to apply the information on AC toxin and on other bordetellae virulence factors, to a more focused study of how AC toxin contributes to infections and diseases caused by this genus. ..
  35. BORDETELLA CYCLASE--STRUCTURE AND BIOLOGICAL ACTIVITY
    Erik Hewlett; Fiscal Year: 1992
    ....
  36. BORDETELLA CYCLASE-STRUCTURE AND BIOLOGICAL ACTIVITIES
    Erik Hewlett; Fiscal Year: 2001
    ....
  37. BORDETELLA CYCLASE--STRUCTURE AND BIOLOGICAL ACTIVITIES
    Erik Hewlett; Fiscal Year: 1999
    ..Additional structural data will be collected with CD spectroscopy an x-ray diffraction studies to develop a better understanding of the actions of AC toxin. ..
  38. BORDETELLA CYCLASE--STRUCTURE AND BIOLOGICAL ACTIVITIES
    Erik Hewlett; Fiscal Year: 2000
    ..Additional structural data will be collected with CD spectroscopy an x-ray diffraction studies to develop a better understanding of the actions of AC toxin. ..
  39. Role of Epithelium in Airway Immunity
    Reen Wu; Fiscal Year: 2010
    ..End of Abstract) ..
  40. Ethanol and ERK/PKB Signaling in Brain
    L Chandler; Fiscal Year: 2007
    ....
  41. The Role of CXCR2 in Pancreatic Cancer
    OSCAR HINES; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  42. MMPs in neural networking
    Katherine Conant; Fiscal Year: 2006
    ..The results of our studies should improve our understanding of those CNS inflammatory diseases that are characterized by elevated levels of MMPs. [unreadable] [unreadable] [unreadable]..
  43. Brain Endocannabinoids and Chronic Stress
    Cecilia Hillard; Fiscal Year: 2003
    ..The successful completion of these studies will enhance our understanding of the processes involved in the adaptation of the brain to chronic stress. ..
  44. Mechanism of renal protection with volatile anesthetics
    H Lee; Fiscal Year: 2009
    ..Our studies will mechanistically advance our understanding of volatile anesthetic-mediated renal tubule protection, leukocyte modulation and improved outcome after renal IR injury. ..
  45. HIV-1 ESCAPE FROM INHIBITION BY CYTOTOXIC T LYMPHOCYTES
    Otto Yang; Fiscal Year: 1999
    ..Use of polyclonal CD8+ lymphocytes from infected individuals will allow evaluation of the inhibitory activity of uncloned CTL and functional comparison to CTL clones. ..
  46. HIV 1 PROTEINS AND DRUGS OF ABUSE AND METALLOPROTEASES
    Katherine Conant; Fiscal Year: 2001
    ..We will also examine the mechanisms by which these substances may affect MMP production, and identify clinically tolerable compounds which may inhibit such production. ..
  47. MECHANISMS OF SYNERGISTIC REGULATION OF STEM/PROGENITORS
    Hal E Broxmeyer; Fiscal Year: 2010
    ....
  48. MMPS IN HIV DEMENTIA: EFFECTS ON BBB STRUCTURE/FUNCTION
    Katherine Conant; Fiscal Year: 2002
    ..Also, the proposed studies will use both primary brain-derived HIV isolates, which differ from laboratory adapted strains, and normal human cells, which also differ significantly from those of rodent or tumor origin. ..
  49. Cytokine Ehancement of Myeloid Progenitor Cell Survival
    Hal Broxmeyer; Fiscal Year: 2002
    ..Use MPC from mice functionally-deleted in proposed intracellular signaling molecules to determine dominant effects of these intracellular molecules in survival/anti-apoptotic events. ..
  50. Myofilament Calcium Sensitivity in Health and Disease
    PAULUS JANSSEN; Fiscal Year: 2007
    ....
  51. Immune Pressure on HIV-1 Nef by Cytotoxic T Lymphocytes
    Otto Yang; Fiscal Year: 2009
    ..To delineate in detail the kinetics of Nef-mediated HLA downregulation versus CTL clearance of acutely HIV-1-infected cells;and 3. To screen for small molecule inhibitors of Nef-mediated HLA downregulation and other functions. ..
  52. Cardiac Contraction-Relaxation Coupling
    PAULUS JANSSEN; Fiscal Year: 2007
    ....
  53. Structural Studies on Bacterial Two-Partner Secretion Systems
    HYEJEONG YEO; Fiscal Year: 2010
    ..We will (i) characterize the HMW1 secretion domain, (ii) characterize the HMW1 C-terminal domain, and (iii) determine the crystal structure of HMW1C, a putative glycosyltransferase. ..
  54. Structural Studies on Bacterial Two-Partner Secretion Systems
    HYEJEONG YEO; Fiscal Year: 2007
    ..We will (i) characterize the HMW1 secretion domain, (ii) characterize the HMW1 C-terminal domain, and (iii) determine the crystal structure of HMW1C, a putative glycosyltransferase. ..
  55. PHYSIOLOGY OF THE PROLACTIN RELEASING PEPTIDES
    Willis K Samson; Fiscal Year: 2010
    ..Additionally, these studies will provide further insight into the coordinated hormonal and autonomic responses to stress. ..
  56. Immune Pressure on HIV-1 Nef by Cytotoxic T Lymphocytes
    Otto Yang; Fiscal Year: 2009
    ..To delineate in detail the kinetics of Nef-mediated HLA downregulation versus CTL clearance of acutely HIV-1-infected cells;and 3. To screen for small molecule inhibitors of Nef-mediated HLA downregulation and other functions. ..
  57. HIV-1 ESCAPE FROM INHIBITION BY CYTOTOXIC T LYMPHOCYTES
    Otto O Yang; Fiscal Year: 2010
    ..Understanding these mechanisms may be important for designing vaccine strategies that promote more effective responses than occur in nature. ..
  58. Cardiac Contraction-Relaxation Coupling
    PAULUS JANSSEN; Fiscal Year: 2008
    ....
  59. Genetic Analysis of Bordetella Pathogenesis
    PEGGY COTTER; Fiscal Year: 2008
    ....
  60. PHYSIOLOGY OF THE PROLACTIN RELEASING PEPTIDES
    WILLIS SAMSON; Fiscal Year: 2008
    ..Additionally, these studies will provide further insight into the coordinated hormonal and autonomic responses to stress. ..
  61. Indiana University Initiative for Maximizing Graduate Student Diversity
    Hal Broxmeyer; Fiscal Year: 2008
    ..D. degrees and pursue careers as independent academic biomedical research scientists/faculty members. ..
  62. REGULATION OF HEMATOPOIETIC CELL PRODUCTION
    Hal Broxmeyer; Fiscal Year: 2008
    ..cancer center, an NIH-designated National Gene Vector Laboratory, an NIDDK Center of Excellence in Molecular Hematology, and state-of-the-art infrastructure, and frequent contacts with active investigators at IU and throughout the world ..
  63. BASIC SCIENCE STUDIES ON GENE THERAPY OF BLOOD DISEASES
    Hal Broxmeyer; Fiscal Year: 2008
    ..abstract_text> ..
  64. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul Lampe; Fiscal Year: 2008
    ..We hypothesize that Cx43 phosphorylation changes are linked to these processes in a way that affects cellular function. ..
  65. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul Lampe; Fiscal Year: 2009
    ..We hypothesize that Cx43 phosphorylation changes are linked to these processes in a way that affects cellular function. ..
  66. HIV-1 ESCAPE FROM INHIBITION BY CYTOTOXIC T LYMPHOCYTES
    Otto Yang; Fiscal Year: 2009
    ..Understanding these mechanisms may be important for designing vaccine strategies that promote more effective responses than occur in nature. ..
  67. PHYSIOLOGY OF THE PROLACTIN RELEASING PEPTIDES
    WILLIS SAMSON; Fiscal Year: 2009
    ..Additionally, these studies will provide further insight into the coordinated hormonal and autonomic responses to stress. ..
  68. Genetic Analysis of Bordetella Pathogenesis
    PEGGY COTTER; Fiscal Year: 2007
    ....
  69. Immune Pressure on HIV-1 Nef by Cytotoxic T Lymphocytes
    Otto Yang; Fiscal Year: 2006
    ..To evaluate the functions of Nefmutants selected by Nef-specific CTL (in vitro); 3. To evaluate potential in vivo selective pressures for conservation of Nef. ..
  70. Proteomic Biomarkers of Health Behaviors
    Paul Lampe; Fiscal Year: 2005
    ..abstract_text> ..
  71. CHEMOKINES/CYTOKINES AND RECEPTORS IN STEM CELL HOMING
    Hal Broxmeyer; Fiscal Year: 2003
    ..These studies should clarity the relevance of SDF-1 and CXCR4 for homing/engraftment and mobilization of primary stem/progenitor cells. ..
  72. Cardiac Contraction-Relaxation Coupling
    PAULUS JANSSEN; Fiscal Year: 2005
    ....
  73. HIV-1 Excape from Inhibiton by Cytotoxic T Lymphocytes
    Otto Yang; Fiscal Year: 2005
    ....
  74. MECHANISMS OF SYNERGISTIC REGULATION OF STEM/PROGENITORS
    Hal Broxmeyer; Fiscal Year: 2001
    ..abstract_text> ..
  75. MECHANISMS OF SYNERGISTIC REGULATION OF STEM/PROGENITORS
    Hal Broxmeyer; Fiscal Year: 2002
    ..abstract_text> ..
  76. Immune Pressure on HIV-1 Nef by Cytotoxic T Lymphocytes
    Otto Yang; Fiscal Year: 2004
    ..To evaluate the functions of Nefmutants selected by Nef-specific CTL (in vitro); 3. To evaluate potential in vivo selective pressures for conservation of Nef. ..
  77. Orexinergic Pathways in Central Autonomic Control
    WILLIS SAMSON; Fiscal Year: 2002
    ..abstract_text> ..
  78. Orexinergic Pathways in Central Autonomic Control
    WILLIS SAMSON; Fiscal Year: 2003
    ..abstract_text> ..
  79. Immune Pressure on HIV-1 Nef by Cytotoxic T Lymphocytes
    Otto Yang; Fiscal Year: 2003
    ..To evaluate the functions of Nefmutants selected by Nef-specific CTL (in vitro); 3. To evaluate potential in vivo selective pressures for conservation of Nef. ..
  80. MECHANISMS OF SYNERGISTIC REGULATION OF STEM/PROGENITORS
    Hal Broxmeyer; Fiscal Year: 2003
    ..abstract_text> ..
  81. Immune Pressure on HIV-1 Nef by Cytotoxic T Lymphocytes
    Otto Yang; Fiscal Year: 2002
    ..To evaluate the functions of Nefmutants selected by Nef-specific CTL (in vitro); 3. To evaluate potential in vivo selective pressures for conservation of Nef. ..
  82. HIV 1 PROTEINS AND DRUGS OF ABUSE AND METALLOPROTEASES
    Katherine Conant; Fiscal Year: 2003
    ..We will also examine the mechanisms by which these substances may affect MMP production, and identify clinically tolerable compounds which may inhibit such production. ..
  83. HIV-1 ESCAPE FROM INHIBITION BY CYTOTOXIC T LYMPHOCYTES
    Otto Yang; Fiscal Year: 2000
    ..Use of polyclonal CD8+ lymphocytes from infected individuals will allow evaluation of the inhibitory activity of uncloned CTL and functional comparison to CTL clones. ..
  84. MECHANISMS OF SYNERGISTIC REGULATION OF STEM/PROGENITORS
    Hal Broxmeyer; Fiscal Year: 1999
    ..4) Evaluate roles for the protein tyrosine phosphatases Syp and PRP-1C in synergistically-induced stimulation of cell proliferation and its suppression by chemokine family members. ..
  85. MECHANISMS OF SYNERGISTIC REGULATION OF STEM/PROGENITORS
    Hal Broxmeyer; Fiscal Year: 2006
    ..3. Elucidate the role of MSAC in proliferation of HSC and MPC, and in self-renewal of HSC, and link these effects to cytokine actions on these functions. ..
  86. CHEMOKINES/CYTOKINES AND RECEPTORS IN STEM CELL HOMING
    Hal Broxmeyer; Fiscal Year: 2005
    ..These studies should clarity the relevance of SDF-1 and CXCR4 for homing/engraftment and mobilization of primary stem/progenitor cells. ..
  87. Genetic Analysis of Bordetella Pathogenesis
    PEGGY COTTER; Fiscal Year: 2006
    ....